LOCUS       DATMNS010000001         2703 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_358289, whole genome shotgun sequence.
ACCESSION   DATMNS010000001 DATMNS010000000
VERSION     DATMNS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358289"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /gene="kynU"
                     /locus_tag="VNW28_00005"
     CDS             complement(<1..1119)
                     /gene="kynU"
                     /locus_tag="VNW28_00005"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HXA08329.1"
                     /translation="MPTSYSADENFALQEDREDPLHALRDRFHIPVGADNKALIYFVG
                     NSLGLMPKATRQIVDQELDDWAKLAVDGHFDAATPWYSYHEALREPAARMVGAQPNEV
                     VCMNSLTVNLHLLMATFYRPTKARNKILMEDPAFPSDTYAIKSQIAHHGFSPNDSLLL
                     ARPRAGEHAIRQEDIETLLEKHGDEIALVLFAGVNFFTGQLFDIVKITAAAQARGCIV
                     GIDLAHAAGNVPLALHDWNVDFAVWCSYKYLNSGPGAVAGAFVHARHATNRDLPRLAG
                     WWGNDPATRFRMQLEPDFVPVPSADSWQLSNPPILAMAPLRTSFAIFDEAGGMAALRA
                     KSTRLTGYLEFLLARAPSNLFTIITPPAPNERGCQLSIL"
     gene            complement(1138..1719)
                     /locus_tag="VNW28_00010"
     CDS             complement(1138..1719)
                     /locus_tag="VNW28_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08330.1"
                     /translation="MNIDAGTLTGITGAILATISLIYARSQAHAQRRQVEEMQRQTEE
                     TRRAQHFETSHALMKDAIAARQDWAVHFRKEWAPPTIGTPLEETLSLLDGNWDLVFRL
                     RAYVEQLQEMFVARKADLVADDHWRAMHWLVRSFFAPEIDRTIFEGFVEMGWVTPEFA
                     KFGREFVVSGIWKDPLGRLEPIGGRSLTTDATR"
     gene            complement(1767..2234)
                     /locus_tag="VNW28_00015"
     CDS             complement(1767..2234)
                     /locus_tag="VNW28_00015"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXA08331.1"
                     /translation="MSSPPFHILAADHTGITVANLERSLAFWRDVLGFEVSHRPHQTG
                     SLASEITGVPGAAISIAVLKGYGHKIELLEYLAPPGRQHVRPRPCDVGSVHVAFVVDN
                     LDAVLSAIAASGWQAAGKAQPLQAGPNAGKRVVYVRDPDGTTIEFMQSLPSKP"
     gene            2413..>2703
                     /locus_tag="VNW28_00020"
     CDS             2413..>2703
                     /locus_tag="VNW28_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXA08332.1"
                     /translation="MTAFADEEVFTRLLEQHAGIIPKVAFGYTSTFADREDLAQEITL
                     QLWRAYPRYAPDRPFSTWMYRIALNVAISFLRRHTHPARQTVSLEEHEFADEK"
BASE COUNT          534 a          800 c          851 g          518 t
ORIGIN      
        1 aagaatcgaa agctggcagc cgcgctcgtt aggcgcgggc ggcgtgataa tggtgaagag
       61 attggaaggc gcacgcgcga gcagaaactc gagatagccg gtaagacggg ttgacttggc
      121 gcggagcgcg gccatcccgc cggcttcgtc gaagatcgcg aaagaggtcc gcaacggcgc
      181 cattgcgagg atgggcgggt tgctcaactg ccaggaatcg gccgagggca caggcacgaa
      241 atcgggctcg agctgcatgc ggaaacgggt cgcgggatcg ttgccccacc aaccggcgag
      301 gcgcggcagg tcgcgattgg tcgcgtgccg cgcatgcacg aaagcgcccg cgaccgcacc
      361 cgggccggag ttgagatatt tgtaggaaca ccagacagcg aaatcgacgt tccaatcgtg
      421 caaggcgagc ggtacgttgc cggccgcatg tgccagatcg atcccgacga tgcagccgcg
      481 ggcctgcgcg gccgcggtga ttttcacgat gtcgaaaagc tgcccggtga agaaattcac
      541 tccggcgaag aggacgaggg cgatttcgtc gccgtgtttc tcgagcaacg tctcgatgtc
      601 ttcctgccgg atggcatgct ccccggcccg cgggcgcgcg agcaggagcg agtcgttagg
      661 cgagaaaccg tggtgcgcga tctgcgactt gatcgcgtag gtgtcggaag ggaaagccgg
      721 gtcttccatc aggattttgt tccgcgcctt ggtcggccgg taaaacgtcg ccatgagcag
      781 gtgaaggttc accgtcaggc tgttcatgca gacgacttcg ttaggttgcg cgcccaccat
      841 ccgcgctgcc ggctcgcgca gcgcctcgtg ataggagtac cagggcgtcg cggcatcgaa
      901 gtggccgtcg accgcgagct ttgcccagtc gtccagttcc tggtcgacga tttgccgtgt
      961 cgccttcggc atcaagccta acgaatttcc cacgaagtag ataagcgctt tgttatccgc
     1021 cccgaccgga atgtgaaagc gatcgcgcaa ggcgtggagc ggatcctcgc gatcctcttg
     1081 cagggcgaaa ttttcgtcgg cggaatagga agttggcatg gtgagttttg aaaacggcta
     1141 acgagtcgca tccgtggtca gcgagcgacc tccgatgggc tcgaggcggc ccagcggatc
     1201 tttccagata ccgctgacga cgaattcgcg cccaaacttt gcgaattccg gggtgaccca
     1261 tcccatttcg acaaagccct cgaagatggt acgatcgatt tccggcgcaa agaaactgcg
     1321 caccagccaa tgcatggccc gccagtgatc gtccgccacg aggtcggctt tgcgggcgac
     1381 gaacatctct tgcagctgtt cgacgtaggc gcgcaagcgg aagaccaggt cccagtttcc
     1441 atcgagcagc gagagcgtct cttccagcgg agttccaatg gtcggcggcg cccattcctt
     1501 tcggaaatgg acggcccaat cctgccgcgc cgcgatcgcg tctttcatca gggcatgact
     1561 cgtctcgaag tgttgcgccc gccgggtctc ttcggtttgc cgttgcattt cctctacctg
     1621 ccgacgttgg gcgtgggcct gggaacgcgc ataaatcagc gagatggtgg cgagaatcgc
     1681 gcccgtgatt ccggttagag ttcctgcgtc aatattcacg cttcggaatc catgatgaat
     1741 gaatcaagcc tgcggaaaag gaggaactaa ggtttgctcg gcagcgactg catgaactcg
     1801 atcgtggtgc cgtcgggatc acgcacataa acaacacgct tgcccgcgtt cgggccggct
     1861 tggagaggct gcgcttttcc cgctgcctgc cagccggagg cggcgatggc gctcaggacg
     1921 gcgtccaaat tatcgaccac aaacgcgacg tggaccgacc cgacatcgca gggacgcgga
     1981 cgaacgtgtt gccgaccggg cggcgccaga tactcgagca attcgatctt gtgcccatag
     2041 cctttgagga cggcgatgga aatcgcggcg ccgggaactc cggtgatttc gctcgccaga
     2101 ctgccggtct ggtgcggtcg atgggagacc tcaaagccga gcacgtcgcg ccaaaaggcg
     2161 agcgagcgct ccaggttcgc gaccgtgatg ccggtgtgat cggcggcgag gatgtgaaag
     2221 ggtggcgagc tcacgtcgtt accgcatcgc ttaagtcgtc ctccgcgcga tttcaaccgc
     2281 ggctcgcgcc ggctgcggag ttctgctcgc ttcgcggctg aattttgagt gtgatttttt
     2341 cgacgtgccc gcgaatcacc tgcacaacaa gaattcagat tgcccgctca gatgcacgcc
     2401 atttctccaa ccatgaccgc ctttgcagat gaggaagttt ttacccggtt gctcgagcaa
     2461 catgccggga tcattcccaa ggtcgcgttt ggctacacga gcacctttgc cgatcgcgaa
     2521 gatcttgcgc aggaaatcac gctccagctc tggcgcgcct acccgcgtta cgcgcccgat
     2581 cgcccattct cgacctggat gtatcgcatc gcgctcaacg tggccatctc gttcctgcgc
     2641 cgccacacgc atcccgccag gcaaaccgtg tcgctcgaag aacatgaatt cgcagatgaa
     2701 aag
//
LOCUS       DATMNS010000002         3524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_716414, whole genome shotgun sequence.
ACCESSION   DATMNS010000002 DATMNS010000000
VERSION     DATMNS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_716414"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..780)
                     /locus_tag="VNW28_00025"
     CDS             complement(115..780)
                     /locus_tag="VNW28_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXA08333.1"
                     /translation="MRFLDRSDAGRFLARELVRFRGGRDLIVLALPRGGVPVGYEIAR
                     ALPAPLDVFVVRKLGVPGREELAMGAVASGGIRVLNAEVVAALGPHAQEAIEWVTARE
                     NEELLRREERYRGARAFPSLVGKTVFLVDDGLATGATMRAAARAVRGRKPARLIIAVP
                     VAAESTCRKMQAEADEVVCGWQPEEFFGIGQFYDNFRQTTDEEVRTLLADAAEERRAW
                     PNE"
     gene            895..2199
                     /locus_tag="VNW28_00030"
     CDS             895..2199
                     /locus_tag="VNW28_00030"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXA08334.1"
                     /translation="MKETGIERTANVPDFTGRPRRVATGGVSWRKTFSAFQYRNYRLY
                     FWGQLVSLIGTWMTNTAQGWLVYQLTGSKALLGVVAAAGTAPMLFFATWGGWAADRFP
                     KRSVLLWTQSAMMILAFVFASLIWSGLIRPWHIIVLGLCGGVAMAFDMPARQSFVVEI
                     ASREDLMNAISLNSAMFNGARIIGPAVAGFLMAKVGIALCFFIDGVSFLAVLGGLLMM
                     RLPAHRGHSAAGGALAQSLEGFRYVWNHTRVRTIFILFAIVGIFGWSYSVLMPAFARD
                     VLHLDEQGYGFLLGASGLGALLGAFTTATFGAGVSARKLAFGGVWLFSAMLLLFSFNR
                     NLIVAMLCLAGSGFGMMLFFSTSNTTVQTIVPDEMRGRVMGIWALIFGGMIPFGSLEA
                     GALAHYIGTPATFGIGACICALAATVTLMIVRRRDAQLAAAA"
     gene            complement(2219..>3524)
                     /gene="gatA"
                     /locus_tag="VNW28_00035"
     CDS             complement(2219..>3524)
                     /gene="gatA"
                     /locus_tag="VNW28_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HXA08335.1"
                     /translation="DAALAAAETADVNLPLGGIPIAVKDAISVAGQPCTCASKILRGY
                     RGIFDATAIARLKAAGAIPFGKTNLDEFAMGSSTENSSVKPTRNPWDPARTPGGSSGG
                     SAAAVAAGEAIASLGSDTGGSIRQPAALSGVVGMKPTYGRVSRYGLVAFASSLDQIGP
                     LTKTVRDAALLLEVMAGVDPHDSTSLDAPVPPYSGALESDLRGVRLGLPKEYLIAGID
                     PEVRAALEAAVAHFRSLGAELVDVSLPHTEYAIDTYYLIATAEASANLARFDGVRYGY
                     RAENPRDLSDHYGRTRAEGFGAEVKRRIILGTYVLSSGYYDAYYLRAQKVRTLIRQDF
                     ARAFEKVEAILSPTTPEPAFLLGERSEDPLRMYLADIFTNAANLAGICGISLPCGFAR
                     EATKYPLPIGLQIMGRALDETRVFQLAHAFEQSTDWHKRRPR"
BASE COUNT          575 a         1177 c         1089 g          683 t
ORIGIN      
        1 gcacctcgtc tacggctggg tcacggagac cgtgcggcgc gcggtgcgca gggcattgta
       61 gaggggcgag gatcgcgccc ggttgggggt ttcgtcctct tagaatcgtt gcctttattc
      121 gttaggccag gctcgcctct cctccgcggc gtccgccaga agggtgcgca cctcctcgtc
      181 ggttgtctgc cgaaaattat cgtaaaactg gccgatgcca aaaaactcct cgggctgcca
      241 cccgcaaacc acctcgtcgg cttccgcctg catcttccgg caggtcgatt cggccgcgac
      301 cggcacggcg atgatcaggc gcgccggttt gcgtcctcgc accgcccggg ccgccgcccg
      361 catggtcgca ccggtggcaa gaccgtcgtc caccaggaac acagttttcc caacgaggga
      421 gggaaaggcc cgcgcgccgc ggtagcgctc ctcgcgccgg agaagttctt cgttctcccg
      481 cgcggtcacc cattcgatcg cttcctgcgc atggggcccg agcgcggcaa ccacctcggc
      541 gttcaagacc cggatcccac ccgatgcgac cgcgcccatc gcgagctcct ctcgcccggg
      601 cacgccgagc ttgcgcacca cgaagacatc gagcggggcc ggcaaggcgc gcgcgatttc
      661 atatcccacc ggcaccccgc cgcgcggcag cgcgagcacg atcaggtcgc ggcccccgcg
      721 aaaacgaacc agctcacgcg cgagaaatcg cccggcatcg ctccggtcca ggaagcgcat
      781 tgcttgcaag gtgaagcgtg aatcgtgcgc caacaattta acaatttagc aatttcacgc
      841 ttccaacact tggtttcccc gcaaatccag acgaggaaat ccgcgccagg cgccatgaaa
      901 gaaacaggaa tcgaacgcac cgccaatgtg ccggatttta ccggccgtcc gcggcgggtc
      961 gcgaccggcg gcgtctcctg gcgaaaaacc ttctcggcct ttcaatatcg caactaccgc
     1021 ctctactttt ggggccagct cgtttctctg atcggcacct ggatgacgaa caccgcgcag
     1081 ggctggctcg tctaccagct gaccggttcc aaggcattgt taggcgtggt cgccgcggcc
     1141 gggaccgcgc ccatgctttt cttcgccacc tggggcggct gggcggccga tcgatttcca
     1201 aaacgctccg tcctcctctg gacacagagc gccatgatga tcctggcgtt tgtcttcgcg
     1261 agcctgatct ggtccgggct cattcggccg tggcacatca tcgtcctcgg actttgcggt
     1321 ggtgtcgcga tggccttcga catgccggcg cgacaatcgt tcgtcgtcga gatcgcgagc
     1381 cgcgaagatt tgatgaatgc gatctcgctg aacagcgcga tgtttaacgg cgctcgcatc
     1441 atcggtcccg ccgtcgccgg tttcctgatg gcgaaagtcg gcatcgcgct ctgctttttt
     1501 atcgatggcg tcagttttct cgcggtcctc ggcgggctcc tcatgatgcg tttgccggcc
     1561 caccgcgggc actcggcggc gggcggcgcg ctcgcccaat cgctcgaggg gtttcgctac
     1621 gtctggaatc acacccgggt ccgcaccatt tttatcctct tcgcgatcgt tggaattttt
     1681 ggctggtcct actccgttct catgccggcc ttcgcgcgcg atgtgctgca cctcgacgag
     1741 caaggctacg gctttttgtt aggcgcgagc ggcctgggtg cgctcctcgg cgccttcacg
     1801 accgcgactt tcggggccgg agtttctgcg cgcaaactgg ctttcggcgg ggtttggctt
     1861 ttctcggcga tgcttttgct tttctcgttc aatcgcaatt tgatcgtcgc gatgctctgt
     1921 ctggccggtt ccgggtttgg catgatgctt ttcttttcca cctccaacac caccgtgcag
     1981 accattgtgc ccgacgaaat gcggggccgg gtgatgggca tctgggcgct gatcttcggc
     2041 ggcatgattc ccttcggcag cctcgaagcg ggcgcgctcg cgcattacat cgggacgccc
     2101 gcgacgttcg gaatcggcgc ctgcatctgc gccctcgccg ccacggtaac gctgatgatt
     2161 gtccgccgac gcgacgcgca gctcgcggcc gccgcctaac gacaccgttt ccctaactct
     2221 accgcggccg ccgcttgtgc cagtcggtgc tttgctcgaa agcgtgcgcg agttggaaga
     2281 cgcgcgtctc atcgagcgcc cggcccatga tttgcagccc gatcgggagc ggatatttcg
     2341 tcgcttcccg ggcgaacccg cagggaagac tgatcccgca aatccccgcc agattggccg
     2401 cgttcgtaaa aatgtcggcg agatacatgc gcaacggatc ctcggagcgt tcgccgagca
     2461 ggaaggccgg ctccggtgtg gtcggcgaaa ggatggcctc caccttttcg aaagcgcgcg
     2521 cgaaatcctg ccggatgagc gtccgcactt tttgcgctcg cagataatag gcgtcgtaat
     2581 aaccggagct gagcacgtag gtcccgagaa tgatccggcg tttcacctcg gcgccgaagc
     2641 cttccgcacg ggtccgaccg taatggtcgg acaagtcccg cggattttcc gcacgatagc
     2701 cgtaacgcac gccgtcgaag cgcgcgagat tcgccgaagc ttccgccgtc gcgatgaggt
     2761 aataggtgtc gatcgcgtac tcggtatggg gcagcgaaac gtcgaccaat tccgcgccta
     2821 acgaacgaaa atgcgcgacc gcggcttcga gcgcggctct cacttccggg tcgatgccgg
     2881 cgatcagata ttccttcggc aatcccagcc ggacgccgcg cagatcgctt tccagcgcgc
     2941 cgctgtacgg tggcaccggc gcgtcgagtg aggtggagtc gtgcgggtcg actccagcca
     3001 tcacctcgag aaggagcgcg gcgtcgcgca ccgttttggt gagcggcccg atctggtcga
     3061 gcgaagaagc aaatgcgacc agcccataac gcgagacgcg gccataggtc ggcttcattc
     3121 cgaccacgcc gctcagcgcc gccggctgcc gaatcgatcc gccggtgtcc gagcctaacg
     3181 acgcgatcgc ctcccccgcc gcgacggccg cggccgagcc gccgctcgac ccgcccgggg
     3241 tgcgcgccgg gtcccacgga ttgcgcgtcg gcttcacgct cgagttctcg gtcgaggatc
     3301 ccatcgcaaa ttcgtccagg ttcgttttgc cgaacggaat cgcgccggcg gctttcagcc
     3361 gggcgatcgc ggtggcgtcg aaaatgccgc ggtaaccacg caggattttc gaggcacagg
     3421 tgcagggctg tcccgcgacg ctgatcgcgt ccttgaccgc gatcgggatt ccacccagcg
     3481 gcaggttgac gtcagccgtc tcggcggcgg cgagcgcggc gtcg
//
LOCUS       DATMNS010000003         3819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3222423, whole genome shotgun sequence.
ACCESSION   DATMNS010000003 DATMNS010000000
VERSION     DATMNS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3819
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3222423"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..1681
                     /locus_tag="VNW28_00040"
     CDS             230..1681
                     /locus_tag="VNW28_00040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXA08336.1"
                     /translation="MSGTRAVLLVLLVWAAIFLPRLGSLEIKSEEGRRILPAVTMLDK
                     GNAAHGAREIFRSYLVPQVGSEPYFRKPPLVNWLVASSFKIFGVRNEWTARLPSVLCV
                     LAVALAFVGVARRSLGPNGSLVAALIWLTNFGLLEKGRMIEIEALYVSLTALAFIYWL
                     SWWKEPGQRWLAWTVPWIFLGLGLLAKGPLNLLFFYAVVIAVLWQARELRRLWSGPHC
                     VGLILMLGIFAAWAIPCLEMVRAGHVATVWSRQFSGRVSGEDFKFGDWVLNIPRAIAY
                     LLPWAVLLPFARFRLLEDTVEQKFCRALSVGLAVPFVLVNLLPGALPRYTMPLLAPAI
                     WLLAIFIRSHALLWPRQLRKAITWTVAATTVAMLLYSLAIIPVLQRREKIRPIAAQLA
                     AAIPPNESLYAVDPDYQPFLFYLPRPIVYVSKVADLPRDARYLLVQPADEKAAEESAL
                     WAPQRAEAILPIQDYRGRRVILLRVQGNSLRPD"
     gene            1709..2182
                     /locus_tag="VNW28_00045"
     CDS             1709..2182
                     /locus_tag="VNW28_00045"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXA08337.1"
                     /translation="MLTMREIDPETIGQLIHESARIWRNKLDQRLRPLGLSQAKWRTL
                     AHLSRGHLTQCDLAQRLSIEEPTLARLLGKLESDRWIKRQCAPHDRRCKTVHLQPKSS
                     ALLQRIEKTARDLRHELLEAIPARDLQICLRVLTDIRNRAASVPTHVMNRNGSPK"
     gene            2179..3366
                     /locus_tag="VNW28_00050"
     CDS             2179..3366
                     /locus_tag="VNW28_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXA08338.1"
                     /translation="MIAASKVGTATRETVGDLEQRFGRKPLYTFAGIAGLVVLFLLIR
                     VLSSGNKNTPPPPPRPVAVATVITKDVPLYLDEIGTCAANESVQVQAQVSGQIVSRDF
                     KDGAYVKKGDLLFMIDQRPYQAALDSAKADNMLAQANLKRQLELKAKDVTATQDLDTA
                     EANAMKTEAAVAAAQVNLDYCSIRSPIDGRAGLRQIDAGNTVTAGTNGSASGAVLVQI
                     DNFDPIYTDFTISEPDIPLVRQYLGGPNLRVLTDAENDNHPALEGHLYFIANTLQPGT
                     GTVQARGITPNPDHLLWPSQFVHVRLILDLIKKARLVPNSAVQIGQNGPYVFVVKKDS
                     TLDLRQVKQGQKQANDLTVIKEGLQPGETVVTRGQLQLAPGTKVVVQETEKTPPADSE
                     GTN"
     gene            3369..>3819
                     /locus_tag="VNW28_00055"
     CDS             3369..>3819
                     /locus_tag="VNW28_00055"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXA08339.1"
                     /translation="MAGSQGNGWDDGERGNGSDNGLPSDRTPAKGEHTAAKKLVQFSS
                     GLSRPFIQRPVMTMLLTASIIVFGTLTYNQLAVNDLPAVDYPVIQVSASYPGANPETM
                     ANSIATPLEKQFTQIPGLALTTSTSSQGNTSITLQFDLSKGIDAAATD"
BASE COUNT          760 a         1205 c         1126 g          728 t
ORIGIN      
        1 accgcctcga tcaggatttc gcgtttgatg ccgcgctccc gctcgaggta atccaacatg
       61 gcgataaact cagcgttcat ggaaattagc gtccaccgac aaaaaagagt gggacaccag
      121 ctcccactcc aatccagcaa cggactaggc cgagaagtta cttttggcga ggcggttcgt
      181 caagttctgc ggtggccgcg gtggacgagc ccgtctagaa tcttcgccga tgtcaggtac
      241 gagggcggtt ctattggtcc tgttggtctg ggcggcgatc tttttgccgc ggctcggttc
      301 gctcgagatc aagagtgagg aaggtcgccg catcctgccg gcggtcacga tgctggacaa
      361 aggcaacgcc gcccacggcg ctcgcgaaat ctttcggagt tacctcgtgc cgcaggtcgg
      421 gagcgagcca tatttccgca aacccccgct ggtcaactgg ctcgtcgcca gctccttcaa
      481 gatcttcggc gtccgcaacg aatggaccgc ccggttgcca tcggtgctct gcgtgctcgc
      541 agtggccctc gccttcgtcg gcgtcgcgcg gcggagcctt gggccaaatg gatcgctcgt
      601 cgctgcgctc atctggctaa ccaacttcgg cctgctcgag aagggccgga tgatcgagat
      661 cgaagcgctt tatgtttcgc tcactgcact ggccttcatc tactggctga gctggtggaa
      721 ggaacctggg cagcgctggc ttgcctggac agtgccgtgg atcttccttg ggctcggcct
      781 cctggccaag ggcccgctta atcttctttt cttctacgcg gtcgtgatcg cagtcttgtg
      841 gcaggcgcgc gagctcaggc ggttgtggag cggaccacac tgcgttggcc tgattctgat
      901 gttgggaatc tttgcggcct gggcgattcc ctgtctggaa atggtgcgcg cgggtcacgt
      961 cgcgactgtc tggtcgaggc agttttccgg ccgggtaagc ggcgaggact tcaaatttgg
     1021 agactgggtg ctcaacatcc cgcgcgccat cgcctacctg ctgccatggg cggtgctgct
     1081 gccctttgcc cgattccggc tcctggagga cacagtggaa caaaaattct gccgcgcctt
     1141 gtcggtcggc ctggcggtgc ctttcgtcct cgtcaacctg ctgcccggcg ctctgccgcg
     1201 ctacacgatg ccgctgctcg cgcccgccat ttggttgctt gcgatcttca ttcgatcgca
     1261 cgccttgctt tggcccaggc aattgcggaa ggcgatcacc tggacggtcg ccgcgaccac
     1321 ggtcgcgatg ttgctttact ccctcgcgat catccccgtt ttgcagcgcc gcgaaaagat
     1381 tcgcccgatc gccgcgcagc tcgccgccgc cattccgcct aacgaatcgc tctacgccgt
     1441 cgatcccgat tatcagccgt tcctctttta cctcccgcgg ccgattgtct acgtgagcaa
     1501 agtcgccgac ctgccgcgag acgcgcgcta ccttttggtc cagccggcgg atgaaaaggc
     1561 ggcggaggaa tccgcgctct gggcaccaca acgggcggag gcgatcctgc cgatccagga
     1621 ttatcgagga cgacgcgtta tccttttgcg ggtgcagggc aattcactcc ggccggattg
     1681 acaaaccggc cgaaacgaat tcgttagcat gctaacgatg agggagatcg accctgaaac
     1741 cattggacag ctcattcacg agtccgcccg catctggcgc aataagctcg accagcgcct
     1801 tcgcccgctt ggcctgagcc aggcgaaatg gcgcaccctc gcgcatctct cccgcggtca
     1861 cctcacccaa tgcgatctgg cgcagcggct cagcatcgag gaaccgactc tcgcccgatt
     1921 gttaggcaaa ttggagtcgg accgctggat caaacgacag tgcgcaccgc atgaccgccg
     1981 ctgcaagacg gtgcacctgc aaccgaaatc gagcgccctc ctgcagagga tcgagaaaac
     2041 cgcgcgtgac ttgcggcacg aacttctgga agcgattccg gcgcgggatc tccaaatctg
     2101 tctgcgagtc ctgaccgaca tccgcaaccg cgccgccagc gtaccgaccc atgtcatgaa
     2161 cagaaacggc agcccgaaat gatcgccgcc agcaaagttg gcaccgcgac gcgcgagacg
     2221 gtcggcgacc tcgagcagcg cttcggccgc aaacctcttt acacttttgc cgggatcgcc
     2281 ggattggtcg ttttgttttt gctcatccgg gttctgagta gcggtaacaa gaacacgccg
     2341 ccgccgccgc cgcggccggt tgcggtggca accgtgatca caaaagatgt cccgctctat
     2401 ctcgatgaga tcggcacctg cgcagcgaat gaaagcgtcc aggtgcaggc gcaggtcagc
     2461 gggcagatcg tctcaagaga ttttaaagac ggcgcctatg tgaagaaagg cgatctgctt
     2521 ttcatgatcg accagcgacc gtatcaggcg gccttggatt cggcgaaagc cgacaacatg
     2581 cttgcccagg cgaatttgaa acggcagttg gaactgaaag ccaaggatgt caccgcgacg
     2641 caggacctcg atacggcgga agcgaacgcc atgaagactg aggcggcggt ggccgcggcg
     2701 caggtgaatc tcgattattg ctccattcga tcgccaatcg acggccgcgc cggcctgcgc
     2761 cagatcgacg ccggcaacac ggtgaccgcg ggaaccaacg ggagtgccag cggggcggtc
     2821 ctcgtgcaaa tcgataattt cgatcccatc tacaccgact ttacgatttc ggagccggac
     2881 attccactcg ttaggcagta cctcggcggg ccgaacctga gagtgctgac ggatgccgag
     2941 aacgacaatc atccggcgct ggaaggacat ctttacttta tcgcgaacac gctccagccg
     3001 ggaaccggaa cggtgcaggc ccgcggtatc acgcccaatc cagaccacct tctatggccg
     3061 tcgcagttcg tccatgtccg tctgattctc gatctcatca agaaggccag gctcgtccct
     3121 aacagcgcgg tgcagatcgg ccagaatgga ccctacgttt tcgtagtgaa aaaggactcg
     3181 acgctcgacc ttcgccaggt gaaacagggg cagaagcagg cgaacgattt gaccgtgatc
     3241 aaagagggcc tgcaaccggg tgaaacggtc gtgacccggg gccagttgca acttgcgccc
     3301 ggaaccaaag tcgtggtgca agagaccgaa aaaactccgc ccgccgactc cgaagggacc
     3361 aactgacggt ggcgggctcc caaggcaacg gttgggacga cggcgagcgg ggaaacggct
     3421 cggacaacgg cctgccctcc gatcgcacgc cggcgaaagg cgaacacacc gcggccaaga
     3481 agctggtaca attctcctcc gggctttcgc ggcccttcat tcagcgcccc gtgatgacga
     3541 tgctgctcac ggcgtcgatc atcgtcttcg gcaccctgac ctacaatcaa ctggcggtga
     3601 atgacctccc ggcggtcgat tacccggtca tccaggtaag cgcctcctat ccgggtgcga
     3661 acccggaaac gatggcgaac agcatcgcga cgccactgga gaaacagttc acccagattc
     3721 ccggtctcgc cctaacgacc agcaccagtt cgcagggcaa cacctcaatt actctgcaat
     3781 tcgatctcag caaaggaatc gacgccgccg ccaccgacg
//
LOCUS       DATMNS010000004         6729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_362950, whole genome shotgun sequence.
ACCESSION   DATMNS010000004 DATMNS010000000
VERSION     DATMNS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6729
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362950"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VNW28_00060"
     CDS             complement(<1..702)
                     /locus_tag="VNW28_00060"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXA08340.1"
                     /translation="MITLLAMHFPISGTDISPVYLVVAGFLIGVMGGFFGVGGSFIAG
                     PALRAVGMHWNFAVGTDLAHIVGKSVVAAKRHRALGNVDLRLGLLMALGTISGAEVGA
                     QLIQMLKRAGNVDFVVSVISIGVYLSISTFMVWESWKTLRLQKRKTPPGQKSPGRGSK
                     DESSFDAVTQAIHRVRLWPMISLPTSGVKSISLWIIVVVSFIGGLFSGFLGGGAGYIR
                     MPMMVYVLGIPTHLAV"
     gene            complement(699..941)
                     /locus_tag="VNW28_00065"
     CDS             complement(699..941)
                     /locus_tag="VNW28_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08341.1"
                     /translation="MNADDRKTLFSFLIELVVYGILVTAYFFLVLHFLGDWLADLDKR
                     SVRLYALLSIGLIIGQSVVLEWVTTSLFRLLRGRSE"
     gene            1081..1314
                     /locus_tag="VNW28_00070"
     CDS             1081..1314
                     /locus_tag="VNW28_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HXA08342.1"
                     /translation="MADVANKYAENVEGKFYVDDQCIDCDLCRETAPANFKRNDDGGH
                     SFVYKQPESPEEEALCKEAMEGCPVEAIGSDGG"
     gene            1384..2193
                     /gene="thiD"
                     /locus_tag="VNW28_00075"
     CDS             1384..2193
                     /gene="thiD"
                     /locus_tag="VNW28_00075"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HXA08343.1"
                     /translation="MAQQSSPVALTVAGSDCSAGAGLQADLKTFSALGVFGVTAVTCV
                     VAEVPGRVLRIEAVDAQNVRDQIELLLAHFPIAAIKTGLLFSPEIVETVATVLEQSAQ
                     GIPVIVDPVMIATSGDALLQPAAIEGYCSRLFPRATLVTPNMAEAAALTGRRVSNLEE
                     MRVAGNELAQKFGTRFLLKGGHLGGAQATDLLFGGTAVAEFSAPFVQGVSTHGTGCTY
                     SAAIAARLALGDSLPEAIARAKEFVSRAIRDHFSWTNSSGEVHALNHERQT"
     gene            complement(2267..2971)
                     /gene="rnc"
                     /locus_tag="VNW28_00080"
     CDS             complement(2267..2971)
                     /gene="rnc"
                     /locus_tag="VNW28_00080"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXA08344.1"
                     /translation="MHPLEERIRYQFRNPHLLSEALTHPSAAHEKTSRRFDNQRLEFL
                     GDAVLQLVITEYLFARYSQAGEGRLTTRRSRLVSRESLKVHAARLDLGRHLLMGRGEE
                     ASGGRKRDSTLGDAFEALLGAIYLDSDLETIRRFILAEMQDDLERLEEEPLEVNPKGQ
                     LQELLQAISPRSPVYEVLAQHGREHEKTFSVRVVWENRMLGEGSGRSKKLAETAAALA
                     ALKEQLWTKHEDPIVP"
     gene            complement(2984..4072)
                     /locus_tag="VNW28_00085"
     CDS             complement(2984..4072)
                     /locus_tag="VNW28_00085"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXA08345.1"
                     /translation="MPRLVLSVSLTIFLAAAAQGEAPVPPSASAALSPAASPALIDTL
                     DAADLARAIPLIKSNYINPAALSETELQRATVAGLISRLGRGVMLLPTRGAAAISTPT
                     PFYREIIDDHIGYLRPGNVNRAQLLELDTTLRGLPGKKVETIILDLRGCSESSDYATA
                     AEFAMRFVPKGKLLFTLRGPDGKQVRAFSSNQDPIYSGFVVLLVDGETVGATEVLAAV
                     VRSYDKAIVIGEPTAGRAVDYSDLPLPSGKILRVAVAEAILPDHQPRFPTGVQPDLPV
                     ALPVAEKEQIFEQSLTKGMAPFVFEADRPHLNEAALLAGTNPEIEAAQQRRARGGDRP
                     TLHDAVLQRAVDLVTSIGVFEKQPGHLP"
     gene            complement(4047..5357)
                     /gene="purD"
                     /locus_tag="VNW28_00090"
     CDS             complement(4047..5357)
                     /gene="purD"
                     /locus_tag="VNW28_00090"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HXA08346.1"
                     /translation="MKLLIVGGGGREHALAWKLQQSPRVEAIFCAPGNAGTAALGENI
                     PLLPNDSRGLVRFAKENQIGLTVVGPDDPLAAGLVDDFQKENLRIFGPTKAAARLEGS
                     KVFAKQLMRCAGVPTAMAGVFDDSRKACRFSGKLHYPVVIKADGLAAGKGVVIAPDAA
                     SAAEAISDMIDRRRFGEAGARVLIEEFLEGWECSLHVLVAGDKYQLLGTACDHKRLQD
                     GNRGPNTGGMGAYSPALGCDADLEKQFHERIMRPLLRALREEKIHFSGLLFPGLMISS
                     GIARVLEFNCRFGDPETQVILPRLKGDLLPLLEATIDGRIDEVKIDLDERRAVTVVMA
                     SAGYPGHVEAGKKISGLDACAQMKNVHIFHAGTRRENGGVFSSGGRVLAVTALGDTMA
                     AARDLAYQAVGKIDFAGCHYRRDIALAAVAANVEATDAASRPQR"
     gene            5605..6000
                     /locus_tag="VNW28_00095"
     CDS             5605..6000
                     /locus_tag="VNW28_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXA08347.1"
                     /translation="MSHRTKGPSVMNAQCLSRVVLSRIADKWTALIIHVLATGTRRYA
                     ELQREIGGISQKMLTQTLRSLERDGLVLRKVHPVVPPKVEYALTPLGRTLIEPLNAIC
                     HWAEKHLPQLEANRARARSMVANGANSRS"
     gene            complement(6075..6302)
                     /locus_tag="VNW28_00100"
     CDS             complement(6075..6302)
                     /locus_tag="VNW28_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08348.1"
                     /translation="MLDEIDRGFVRLEPRPAFFAVDRAIVFHSEFPNNKGKRQALEDE
                     RRENDTKGKKQNVATTVDRRAVGEHERESKQ"
BASE COUNT         1317 a         2054 c         2059 g         1299 t
ORIGIN      
        1 gaccgcgaga tgggtcggaa tgccgaggac ataaaccatc atcggcatcc ggatgtagcc
       61 cgcgcccccg ccgaggaaac cgctgaataa ccctccgata aacgagacaa ccacaatgat
      121 ccagagtgaa atcgacttta cgccggaggt tggcaatgaa atcatcggcc acaaacgaac
      181 gcgatgaatc gcctgtgtca ccgcgtcgaa agacgattca tcttttgacc cgcgcccagg
      241 gctcttttgt ccggggggcg tttttctctt ctggagtcgg agcgtcttcc agctttccca
      301 caccatgaaa gtggaaatgc tcaagtagac gccgatcgag atcacgctaa ccacgaaatc
      361 aacattgccc gcgcgcttca gcatttgaat aagctgcgcg cccacttcgg ccccgctgat
      421 tgtcccgagc gccatgagca atccgagccg caggtcgacg ttgcccaacg cccgatggcg
      481 cttcgcggcg acgaccgatt ttccgacaat gtgcgcgaga tcggtgccga ccgcgaaatt
      541 ccaatgcatt ccgactgcgc gcaacgcggg cccggcaatg aagctcccgc cgactccgaa
      601 aaaaccaccc atgactccga tcagaaaccc ggcgacaacc aggtagaccg gactgatatc
      661 cgttccggag atcgggaagt gcatcgccag cagggtgatc attcggaccg gccgcgcagc
      721 aggcggaaca aggaagtggt aacccactcg aggacgacgg attgcccgat gatcagaccg
      781 atcgaaagca aggcgtagag tcgaacgctc ctcttgtcga ggtcagcgag ccagtcgccg
      841 aggaagtgca agacgagaaa aaaataggcg gtgaccagaa tgccgtaaac gaccaactcg
      901 ataagaaagg agaagagtgt cttgcggtca tcggcattca taaggtcaag gcggaagctg
      961 gtcagacagt tcggccttcg ctcatttggc ggttgcgctg caacgcgtca accgaaaatc
     1021 ctttgcgcgg cgaatgaatc ttcctacctt cgtggcctcg gatcttccgg gggtggattt
     1081 atggctgatg tagcgaacaa gtatgctgaa aacgtggagg ggaaattcta cgtcgatgat
     1141 caatgcatcg attgcgatct ctgccgcgaa acggcgcccg cgaacttcaa gcggaacgac
     1201 gacggaggcc attcgtttgt ctacaagcag cccgaatcgc ccgaggaaga ggccctctgc
     1261 aaggaggcca tggaaggttg cccggtcgaa gcgatcggca gcgacggcgg ataggcccgt
     1321 gaatcgaggt gcgagattcg tgattcggct tcgattcacg atctaacgac tctcgacgcc
     1381 accatggccc agcagtcatc tcctgtcgcc ctcaccgtcg cggggtcgga ttgcagcgcc
     1441 ggggcgggtt tgcaggccga cctgaaaacg ttcagcgcgc tgggcgtttt cggagtaacc
     1501 gcggtgacct gcgtggtcgc tgaagtgccg ggaagagttt tgcgaatcga ggcggtcgac
     1561 gcgcagaacg tgcgtgatca aatcgagctg cttctggccc attttccgat cgcggcgatc
     1621 aaaaccgggc tgctcttttc cccggagatc gttgagaccg tcgcaaccgt cctcgagcag
     1681 tcggcccagg gaattccggt cattgtcgat ccggtcatga tcgcgaccag tggtgacgcg
     1741 ttgctccaac cggcggcgat cgagggttat tgttcgcgtc tttttccgcg cgcgacgctg
     1801 gtcacgccga acatggcgga agcggccgcc ctgaccgggc ggcgcgtgag caatctcgag
     1861 gagatgcggg ttgccgggaa cgaactggcg cagaaattcg ggacgcgatt cctactcaag
     1921 ggtgggcatc tgggtggtgc tcaggcgacc gatttgctat ttggaggaac ggccgtcgcg
     1981 gaattctcgg cgcctttcgt tcaaggcgtt tcgacgcacg gcaccggctg cacctattcc
     2041 gcggcgattg ccgctcgtct ggcgctgggc gattcgctgc cggaagcgat tgcgcgggcc
     2101 aaggagttcg tcagccgggc gatccgcgac catttttcct ggacgaattc atcgggcgaa
     2161 gtccacgcgc tgaatcacga acggcaaacc tgatcggagg gcgggcgctg tcccgtccgc
     2221 gttttagaaa tcgaggacaa cacggaggtc gtccctccaa gtttcgctag ggcacgatcg
     2281 ggtcttcatg tttcgtccag agctgttcct ttaaggcggc gagggccgcg gccgtttccg
     2341 ccaatttctt actccggccg cttccttcgc cgagcattcg attttcccag acgacgcgaa
     2401 cggagaatgt cttttcgtgc tcgcggccat gttgagcgag cacttcgtaa accgggctgc
     2461 gcggcgagat ggcctgcagc agttcctgaa gctgcccctt ggggttcacc tccagcggtt
     2521 cctcctcaag gcgttccaga tcgtcctgca tttctgccag aatgaaacgg cggatggtct
     2581 cgagatcgct atcgagataa atggcgccga gcagcgcttc gaaggcgtcg cccagcgtgg
     2641 aatcgcgctt ccggcccccg ctcgcttctt ccccgcgacc catcagcaga tggcgcccga
     2701 gatcaagccg ggcggcgtgc accttgagcg attcgcgcga gacgaggcgc gaccgccttg
     2761 ttgttaggcg gccttccccg gcctgactgt agcgcgcaaa gagatactcg gtgatgacga
     2821 gctgcaagac cgcatcgccg aggaattcga gacgttgatt gtcgaaacgc ctgctcgttt
     2881 tttcatgggc ggcgctcgga tgggtgaggg cctcgctcag caggtgcgga ttgcgaaact
     2941 gatatcggat acgttcttcg agaggatgca cggaacgtcg tcgttagggc aagtgacccg
     3001 gctgcttctc gaacactccg atcgaggtca ccaggtcgac ggcgcgttgc aggaccgcat
     3061 catgcagggt gggtctgtcc ccgccccggg cgcgtcgttg ctgcgcggct tcgatctccg
     3121 gattggtgcc cgcgagaagg gcggcttcgt tgaggtgcgg ccggtcagct tcaaaaacaa
     3181 agggggccat tcctttcgtt aggctctgct caaaaatctg ttccttctcc gccaccggca
     3241 gcgccaccgg caggtccggt tgcacgccgg tgggaaagcg cggctggtga tccggcagga
     3301 tcgcctcggc caccgcgacg cgcagaattt ttccgctcgg cagaggcaaa tcggagtagt
     3361 cgaccgcgcg tccggcggtg ggttcgccaa tgacaatggc cttgtcgtag gagcggacga
     3421 cggcggccag cacttccgtt gcgccaaccg tttccccgtc cacgagcagg acgacgaaac
     3481 cgctgtaaat cggatcctga ttggaggaaa atgcgcgcac ctgtttgccg tccggtccgc
     3541 gcagggtgaa aagaagtttg cctttcggga caaaacgcat cgcgaactcg gccgcagttg
     3601 cgtagtcgct gctctcgcta catccgcgca gatcgagaat gattgtttcc acttttttgc
     3661 cgggcaggcc gcggagcgtt gtgtccaatt ccaggagctg cgcccggttc acatttcccg
     3721 ggcgcaaata gccaatgtgg tcatcgatga tctcgcgata aaatggcgtt ggcgtcgaga
     3781 tggcggcagc cccgcgggtc ggcaagagca ttaccccacg accaagacgg ctgatcagac
     3841 cggcgacggt ggctcgctgc agctccgtct cgctcagcgc cgcgggattg atgtaattgc
     3901 ttttgatgag cgggatggct cgcgcgagat ccgccgcgtc gagggtatca atgagggcgg
     3961 gggaagcagc gggagaaaga gcggccgagg cggaaggcgg aaccggggct tcgccttgcg
     4021 ccgcggccgc caggaaaatc gttaggctaa cgctgaggac gagacgcggc atcggtcgcc
     4081 tcgacgttag ccgcaactgc ggcaagagca atgtcgcgac gatagtggca gcctgcaaaa
     4141 tcgattttcc cgacggcttg gtaagccagg tcgcgcgctg ccgccatcgt gtcgccgagc
     4201 gccgttacgg ccaggacccg accgccgctg ctgaaaacgc cgccgttctc ccggcgtgtc
     4261 ccggcatgga aaatgtgcac gttcttcatc tgcgcgcagg cgtcgagacc ggagattttc
     4321 ttccccgctt cgacgtggcc cgggtagccg gccgacgcca tcaccaccgt gacagcccga
     4381 cgttcgtcca ggtcgatttt cacctcgtca atccgcccgt cgatggtcgc ttccagcagc
     4441 ggaagcaggt cgcctttgag ccgtggcagg atcacctgtg tctcgggatc accgaaacgg
     4501 cagttgaatt ccagcacgcg cgcgatcccg ctgctgatca taaggccagg gaagagcagc
     4561 ccggaaaaat ggattttttc ctcccttaaa gcacggagga gtgggcgcat gattctctcg
     4621 tgaaattgtt tttccagatc ggcatcgcag ccgagcgccg gactgtaggc gcccatcccg
     4681 ccggtgttcg ggccgcggtt tccatcctgc agccgcttgt gatcgcaggc cgtgccgagc
     4741 agttgatact tgtcgcccgc gaccagaacg tggagcgaac actcccagcc ttcgagaaat
     4801 tcctcgatca ggacgcgcgc gcctgcctcg ccgaagcgcc gccggtcgat catgtcggag
     4861 atggcttccg ctgcgctcgc ggcatcgggc gcgatcacca cccccttgcc ggcggcgaga
     4921 ccgtcagcct tgatcaccac cgggtaatgc agtttcccgg aaaaccggca cgccttcctg
     4981 ctgtcgtcga aaaccccggc catcgccgtc ggcacgccgg cacagcgcat cagctgtttt
     5041 gcaaaaacct tcgaaccctc cagtcgcgcg gcggccttgg tcggcccgaa aatgcgcagg
     5101 ttctcctttt ggaagtcatc caccaggccc gcggcgagcg gatcatccgg gccgacgact
     5161 gtcagcccaa tctggttttc tttcgcaaac cggacgagcc cgcgggagtc gttaggcaag
     5221 agcgggatgt tctccccgag tgcggccgtc ccggcattgc ccggcgcgca gaaaatcgct
     5281 tcgacccggg gcgattgctg cagcttccag gcgagcgcgt gttcgcggcc gccacctccc
     5341 acgattaata acttcatcgc ggccgccgtc ctgtttctgg tctccccata ttcgcgatat
     5401 ccacaggcga ctctccgtca tcggaagcga ggttgctgat ttttcaggcc cctggggaac
     5461 gctggaaggg agacgtatca gaaatgcgtt ccttgccaag caaaatcggc agctgcgctc
     5521 caaaccctca tttacgccca tacgcatctc ttggattgtt actctcgcat ttgcgcgtgc
     5581 cgacggattc cgttaccaaa tcccatgtcc catcgaacca aaggaccgtc ggtcatgaac
     5641 gcacagtgtc tctcccgcgt cgtcctgagc cggatcgccg acaagtggac tgcgctcatc
     5701 attcacgtcc tcgcgaccgg gacgcgccgt tatgccgagc tgcaacgcga gatcggcggg
     5761 atttcgcaga agatgctcac ccagaccttg cgcagtctgg aacgcgacgg cctcgtcctg
     5821 cgcaaagtgc atccggtcgt tccgccgaag gtcgagtacg cgctcacccc gctcgggcgg
     5881 accttgatcg agccgctgaa cgcgatctgc cattgggcgg aaaaacattt gccgcagttg
     5941 gaagccaacc gcgcccgggc ccggtcgatg gtggccaacg gcgcgaactc gcgcagctag
     6001 gccgacggtc ggggccggag ccgtgcatca cgccgaacct atcgtccgcc tgctcgtgtt
     6061 ggtggcgggg ctcgtcattg tttgctctcc cgctcgtgct cgccgacggc ccgcctgtcc
     6121 acggtcgtag ctacattttg tttcttaccc tttgtgtcat tctcacgacg ctcatcttcc
     6181 agggcctgac gcttcccctt gttattcgga aactcggagt ggaagacgat ggcgcgatcg
     6241 acagcgaaga acgccgggcg tggctcgagg cgaacgaagc cgcggtcgat ttcatcgagc
     6301 aaaaagcgct cgaagaaaag ctccccgagg atgtcatcgc ccggtgcgcg ccgagtgttg
     6361 cgaccgcatc gcgcagctca acgcctgtgg tgaggaaagc agaaatcccg gcggagtcgt
     6421 ggccacgccg gtctactagc gattacaaca tggcgccctg cgggtcgaac gccggaccat
     6481 catcgcgctc cgcaattcgc agcagatcaa tgacgaggcg ttccgccgca tccagcgcga
     6541 tcttggcctc gccgaagcgc ggttgacggg agactaaaat ttagcttgcc aatggccgcc
     6601 gggtgggagc aaccattaag gatgaaaaat aaactcctca gaaacacgag agctttgggc
     6661 ttggcagggt tgctcatttt tgtctgcccc ggctgttcca agcagcaagc cttgcaagag
     6721 cacagagga
//
LOCUS       DATMNS010000005        12356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1794091, whole genome shotgun sequence.
ACCESSION   DATMNS010000005 DATMNS010000000
VERSION     DATMNS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 12356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12356
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794091"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VNW28_00105"
     CDS             <1..1410
                     /locus_tag="VNW28_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXA08349.1"
                     /translation="LVVATGDPLDFDSIDSLSFLLKREIELVCTTAEKIHEALIKYYG
                     TAEEGVDVLGQKIGKAGLEEIDVAEGAELAEGKGGDAPIIRMVSMLLLEAHKLGASDI
                     HLEPLEKSFRVRFRIDGVLHEMQSPPKKLQSAIISRLKIMTGSMSIAEKRLPQDGRIQ
                     VRMGRKSIDLRVSTILTNHGESMVMRLLDKTSLLRGLSELGFLSDDQETFEHLLRAPD
                     GILLVTGPTGSGKTSTLYACLNYINKPDRKIITIEEPVEYQMNGINQVQVNSEIGMTF
                     PVALRSILRQAPNIIMIGEIRDLETASIATNASLTGHLVFSTLHTNDAPSAVARLIDI
                     GVQPFLVATAIRAIIAQRLVRRLCVNCQEPGQLLDSERRALHIEARQLGEANVMRPVG
                     CERCRQTGYKGRLGIFEIFEIDDAARHMVNNRASTLQLRQRARELGMRTLREDGVRKV
                     LAGLTSAEEVISITLGDLN"
     gene            1552..3240
                     /locus_tag="VNW28_00110"
     CDS             1552..3240
                     /locus_tag="VNW28_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXA08350.1"
                     /translation="MNNERLADLFVDQGLMDRAQADDVLLETTQNGKAIDQAMVDHGI
                     VDEHQFYLAIAEALGTEVVNLEQIKFTPRIIHLIPAGLARLHRALPIGEQDNGIRVAL
                     VDPLDTQTIEDLRFALGRDIRVVLAPSLQIEERLKKHYGEDTSSMEEILKQLGEAGEL
                     MTLAGEKALGLNVEAEANATPIIRFVDLILYQAIQDRASDIHFEPFEAEFKVRYRVDG
                     ALYEMAPPPRHLALPVISRVKVMANMNIAERRLPQDGRIQKNIAGRVVDLRVSTLPTQ
                     FGESVVLRVLDRSTVNLDLEALGMPDYILQYILEVIHRPNGIFIVTGPTGSGKTTTLY
                     SCLRRINTIDSKLMTAEEPVEYDLEGVMQVPVNDAIGLSFARVLRAFLRQDPDRIMVG
                     ETRDLETAQIAIQASLTGHLVFTTLHTNDAPGAIARLIDMGVEPFLISSTLDAVLGQR
                     LLRSICRDCRTAYQPNESLLAQLNLSPREIGDRHFYYGKGCEKCNRTGYKGRKGIYEL
                     MRITDPLRALINERVPSVVLKQKAIELGMTTLRQDGVRSIFEGDTTIEEVLKYT"
     gene            3421..4707
                     /locus_tag="VNW28_00115"
     CDS             3421..4707
                     /locus_tag="VNW28_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXA08351.1"
                     /translation="MPRFTYIALDSRGQESTGLVDAPSTNDAVGQLRQAGYFPTSVFE
                     NGPDGVAEKKDRRATKTTAAAPRAKGKGIVLFAKKTVKARVLMVFTRQLATLIDAGLP
                     LLRGLNVLAKQERDLLLKSTIHLLADSVQGGSTFSESLAQHPRIFNKLFVNMVKAGEL
                     GGVLELVLTRLAEFQEKAQKVKNKVKSAMIYPAIVLSLAMVIMAFLLVFIVPKFESIF
                     HDMLGNKPLPAITQFVIGISGFMQHHWLIILGAIFVILAGYKFISRTPAGRAVIDRLA
                     LRAPLFGDLTRKSSISRFARTLGTLVTSGVPILEALNITRETAGNAVIANAISQVHHS
                     VKEGESIVQPLESSGAFPPMVISMVDVGEETGQLPEMLLKIAEVYDDEVDNAVAGLTS
                     MLEPIMIVLLAVIVGTIVIALFMPLISIIGGLQAQG"
     gene            4815..5360
                     /locus_tag="VNW28_00120"
     CDS             4815..5360
                     /locus_tag="VNW28_00120"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXA08352.1"
                     /translation="MIRHSSFRACRGFTVIELLVVMAITLVLAGLVLTTSSYVHNKGA
                     RSRAEAEIAAMSAALENYKADNGVYPISGATSALNARTFTDPRDYQSASAALYQSLAG
                     DSDTDGLPGPGVSYMAFKPNQLSGSGSTIHIKDPFGNSYGYSTAGQAGTSGVGYNPTF
                     DLWSTAGSFTDSGKSGWIKNW"
     gene            complement(5478..6512)
                     /locus_tag="VNW28_00125"
     CDS             complement(5478..6512)
                     /locus_tag="VNW28_00125"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXA08353.1"
                     /translation="MNAFTARPVAPLPVWSGRSKQRNWLLVGLLLSLTLHGLLCFFFY
                     RTGFQPTAPLGMEKPPPPTFKVKSVEVAPKPLDQASSSDAAEAAKPNPDQTDVQLPDD
                     QKSFDKLLQEVHATAALPDDTRDVLPDKPQVEQTNLNSVINEIERTTAQSLEKTPDAQ
                     RQQAPSNDVADSGRPQPAMTGTELATSTTIKRPNTFNGLPGDSAGPRTDRAPGFSDLE
                     SLLSQKGPLGAGTKFKMPNNQLFEYDSAELQGSAVEQLQKVATLIKRNPNATFTLEGY
                     TDSYGSDEYNLDLSRRRAESVKAYLVEALGINPEQIETRGYGKANLLTSPDASIPEQE
                     INRRVVVVVH"
     gene            complement(6689..7444)
                     /gene="panB"
                     /locus_tag="VNW28_00130"
     CDS             complement(6689..7444)
                     /gene="panB"
                     /locus_tag="VNW28_00130"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HXA08354.1"
                     /translation="MEDFRERKRRRERITALTAYDYPTARLLDESGIDIILVGDSLGM
                     VVLGYTDTTEVKFEEMLHHTRAAARGVKRALLVADLSIGSYDTPEAAVANARQLVEAG
                     AHAVKLEGGASHVPQIRAIVGAGIPLMAHIGMLPQSVRAEGGYKVKGRGAAEAERLLA
                     DARAVEEAGAFSVVLEIVASEAARQITGAIGIPTIGIGSGEHCDGQILVTHDLIGLYP
                     WFTPKFVSPAAQVAEEIRRAARIFIERTRGSIR"
     gene            complement(7456..7956)
                     /gene="folK"
                     /locus_tag="VNW28_00135"
     CDS             complement(7456..7956)
                     /gene="folK"
                     /locus_tag="VNW28_00135"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HXA08355.1"
                     /translation="MRAGIALGSNLGDRLSCLTNARARIFALPEVSPPLLSSALYETE
                     PVECEPGAKKFLNAVVEIGYTGASSRLLEELNKIEAALGRPAEHARNHSRTIDLDLLY
                     HGARVIDEPLLQLPHPRLQLRGFVIRPLAEIRPNLVLPGHARTVGELSEELTHGPSVV
                     RSARQW"
     gene            complement(8014..8697)
                     /gene="dapB"
                     /locus_tag="VNW28_00140"
     CDS             complement(8014..8697)
                     /gene="dapB"
                     /locus_tag="VNW28_00140"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HXA08356.1"
                     /translation="MSVTRILLVGANGRMGQAVAAAAAKDTSLTISARIERGDPVARV
                     IENCDAVIDFSDADATEAVCQACAQHGKPLVLGTTGHSAAQKEAVAAVAQKIPLVFAA
                     NFSVGVNVLFALTRRAAELLGDDFDLEVVELHHRTKKDAPSGTAKRLLEILQEMRGGH
                     PAVPAHSIRAGDIVGEHTVIFAGAGERLELTHRASSRETFAVGALRAVRWALKQPPGL
                     YNMENVIGL"
     gene            complement(8694..9575)
                     /gene="dapA"
                     /locus_tag="VNW28_00145"
     CDS             complement(8694..9575)
                     /gene="dapA"
                     /locus_tag="VNW28_00145"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXA08357.1"
                     /translation="MFRGTYTALVTPFQNDEVDYEAFARLIEAQIAAGITGIVPVGTT
                     GESPTLSHGERRQVIELAVQAARKRCQVIAGTGSNSTRDAIAHTREAEQIGVDGALLV
                     APYYNKPSQEGLFRHFAAIAESTSLKLVLYNIPGRCGVDIASDTVVRLAENCPNIVAI
                     KEASGSVDRVSELVGRLPDGFTVLSGDDSLTLPFLAVGAAGVVSVASNLFPAEVSQMV
                     RLSLEGKAEEARTLHRKFFALFKDIFIEPNPVPAKTILAWRGVMSAECRLPLVGMTEA
                     NSARLRKTLDALEKTGG"
     gene            complement(9614..10417)
                     /gene="dapF"
                     /locus_tag="VNW28_00150"
     CDS             complement(9614..10417)
                     /gene="dapF"
                     /locus_tag="VNW28_00150"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HXA08358.1"
                     /translation="MLRFTKMNGAGNDFVLLDNRAGDLALTREQIARVCDRHRGVGAD
                     GVLLLERATNGADFRMRYYNADGGEAEMCGNGARCFARFADRTAGPRRRLSFETPAGV
                     IGATLKGELVTLQMSEPQDMRLGLEVPVGGEKIRADYIDSGVPHVVVPVAKIDDVDVR
                     SLGSALRHHPMFEPRGANANFSERRGPRGIAIRTYERGVEDETLACGTGVVASALIFA
                     ANENVAGPIDILVRGGDTLQVDFTRRGDQFSNVTLTGPADFVFEGTIEL"
     gene            10578..11342
                     /locus_tag="VNW28_00155"
     CDS             10578..11342
                     /locus_tag="VNW28_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08359.1"
                     /translation="MRFATALALAALCCAVDSAPAQLPRSPYNALILEQIKEMPAGGK
                     YSASSTATTRLQSAVQFAAGKLFIQPITAMPSYCSGATYLVFLKTIAALRDRGALSLD
                     DRTLNQLLIRGQRDGAGIWGRWNANGPGTARLFHELGLGRNFDDFEAAQPGDFMKIFW
                     SPEVGRAEHGHSVIFLRMEKKEGIDYVRYWSSNIPSGYGERSVPRAKIVRAIFSRLET
                     PSNLSRVGEVPKLDPYLASLERVRSSYEEAKEKCGM"
     gene            11531..12190
                     /locus_tag="VNW28_00160"
     CDS             11531..12190
                     /locus_tag="VNW28_00160"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXA08360.1"
                     /translation="MLDQEKKLTINEIYLSVQGESTWAGLPCVFVRLTFCDLRCTYCD
                     TEYAFYEGKKQSLGEIVAAVTSYDCPLVEITGGEPLLQKNVLSLMTLLADAGRTILLE
                     TSGAHDISGVDPRVHRIMDLKTPGSGEVARNLFSNIAHLTPRDEVKFVIGSREDYEWS
                     RTRVHEHRLAERCRAVLFSPIFGRIDPRQIVEWILADHLPVRFQLQMHKFIWTPTQRG
                     V"
BASE COUNT         2376 a         3865 c         3711 g         2404 t
ORIGIN      
        1 ctcgtcgtcg cgaccggaga cccgctcgat ttcgacagca tcgacagcct cagctttttg
       61 ttgaaacgcg agatcgaact ggtctgcacc acggcggaga aaatccacga ggccctgatc
      121 aagtactacg gtaccgcgga agaaggggtc gacgtcttgg gccagaaaat cggcaaagct
      181 gggcttgagg aaatcgacgt cgcggaaggc gccgaattgg ccgaaggcaa gggaggcgac
      241 gcgccgatca ttcgcatggt ttcgatgctc ctgctcgagg cgcacaagct cggcgcgagc
      301 gacatccacc tcgagccgct tgagaaaagc ttccgcgtcc gcttccggat cgacggcgtg
      361 ctgcacgaaa tgcaatcgcc gccgaagaag ttgcagtccg ccatcatcag ccggctcaaa
      421 atcatgaccg gctcgatgag cattgcggaa aagcgtcttc cgcaggacgg ccgcatccag
      481 gtaaggatgg gccgcaaatc gatcgacctc cgtgtctcga ccatcctgac caaccacggc
      541 gaaagcatgg tgatgcgcct gctcgacaaa acgagcttgc tccgcggtct gtccgagctg
      601 ggcttcctga gcgacgacca ggaaaccttc gaacaccttc tgcgcgcgcc cgatggtatt
      661 ttgctggtca ctggcccaac cggctctggc aagacgagca cactctacgc ctgcctcaac
      721 tacatcaaca aacccgatcg caagatcatc accatcgagg agccggttga ataccagatg
      781 aatgggatca accaggtgca ggtgaattcc gagattggca tgacctttcc ggtggcgctt
      841 cgttccatcc tgcgccaggc gccgaacatc atcatgatcg gcgaaattcg cgatctcgag
      901 acggcgtcga tcgcgaccaa cgccagcctc accggtcacc tcgtcttcag caccctgcac
      961 accaacgacg cgccctcggc cgtcgcgcgc ctgatcgata tcggcgtgca gccgtttctt
     1021 gtcgccaccg cgatccgcgc gattatcgcg caacggctcg tgcggcgact ttgcgtcaac
     1081 tgccaggaac cgggtcagct cttggattcg gagcggcgcg cgttacacat cgaggctcgg
     1141 caactgggtg aggcgaacgt catgcggccg gtcggttgcg aacgctgccg gcagaccggc
     1201 tacaaaggcc ggctgggcat tttcgagatc ttcgagatcg acgacgcagc gcgccatatg
     1261 gtgaacaacc gcgcctcgac tttgcagctg cgccagcgcg cccgcgagct cggcatgcgc
     1321 accctgcgcg aggatggcgt gcgcaaggtg ctggccggcc taacgagcgc ggaggaagtg
     1381 atttcgatca ctctgggtga tttgaattga atgacgaatt ttcggatgcc caatgacgaa
     1441 ggaatgccca atgacaaatg atgaaatgag atacgcgcgc tgcgagttct ttcgtcattc
     1501 gagcttcgtc attcctccgt caatcagcat tcgtcattcc tcattttcca cgtgaacaac
     1561 gaacgactcg ccgatctttt cgtcgaccag ggcctgatgg accgggccca ggccgacgac
     1621 gttctgctcg agacgaccca gaatgggaaa gcgatcgacc aggcgatggt cgatcacggg
     1681 attgtcgatg aacatcaatt ctacctcgcg attgccgagg ccctcgggac cgaggtggtg
     1741 aatctcgaac agatcaaatt cactccgcgg attatccatc tcatcccggc cggcctcgcg
     1801 cgtttgcatc gcgcgctgcc tatcggcgaa caggacaacg ggattcgcgt cgcgctcgtc
     1861 gatccgctcg atacgcagac gatcgaagac ctgcgctttg ccttgggccg cgacatccgc
     1921 gtcgtcctcg cgccttcgtt gcaaatcgaa gaacgcctca agaagcacta cggcgaggac
     1981 acctccagca tggaggaaat cctcaagcag ctcggcgaag ccggcgagct tatgaccctg
     2041 gcgggcgaga aggcgctcgg tctgaatgtg gaggccgaag cgaatgccac cccgatcatt
     2101 cgcttcgtcg atttgattct ctatcaggcg atccaggacc gggcgagcga catccatttc
     2161 gagccgttcg aggccgagtt taaagttcgc taccgagtgg acggcgcgct ctacgaaatg
     2221 gccccgccgc cgcgccatct cgcgctcccc gtcatctcac gcgtcaaagt gatggcaaac
     2281 atgaacatcg ccgagcgccg cctcccgcag gatggacgca tccagaaaaa catcgcgggc
     2341 cgcgtggtcg atctgcgcgt ttcgactttg ccgacgcaat ttggcgaaag cgtcgtcctg
     2401 cgcgtgctcg atcgctcgac cgtcaatctc gatctcgagg ccctcgggat gccggactac
     2461 atcctgcagt acatccttga ggtgattcat cggcctaacg gaatcttcat tgtcaccggc
     2521 ccgaccggtt ccgggaaaac gaccacgctt tattcctgcc tgcgcaggat caacacgatc
     2581 gattccaaac tcatgaccgc ggaagagccg gtcgagtacg acctcgaggg cgtaatgcag
     2641 gtgccggtga atgacgccat cggcctgagt ttcgcccgtg tcctgcgcgc cttcctccgc
     2701 caggatcccg accgcatcat ggtgggcgag acgcgcgatc ttgagacggc gcagatcgcg
     2761 atccaggcct cgcttaccgg gcatcttgtc ttcaccaccc tgcacacgaa cgatgccccc
     2821 ggcgcgatcg cgcgtctcat cgacatgggc gtcgagccgt tccttatttc ctccaccctc
     2881 gacgccgtcc tcgggcaacg tctgctccgg agcatctgtc gcgattgccg cacggcctac
     2941 cagcccaacg aatcgctcct cgcccagctc aacctttcgc cccgcgaaat tggcgaccga
     3001 catttttact acggcaaggg ctgcgagaaa tgcaatcgca ccggctacaa gggccgcaaa
     3061 ggcatttacg agttgatgcg catcaccgat cccctccgcg ctttgatcaa cgagcgcgtt
     3121 ccctcggtcg tcctgaaaca aaaggcgatc gaactcggca tgactaccct gcgccaggac
     3181 ggcgtgcgca gcatcttcga aggcgacacc acgatcgagg aagtgctcaa gtacacttga
     3241 ggaaatgacg aataacgaat gtctaatgcc ggaggaaaga cgaagcccaa atgacgaagg
     3301 aggtcgtcac gcgatcattt tgtcattcca tcgtccttcg tcactcagaa ttcgtcatct
     3361 ccttcccctt ctcacaagat ctcgacacaa ctttccctcc tctctatcct tcccgccgtt
     3421 atgccgcgct tcacttacat agcgctcgac tcgcgcggtc aggaatccac cggtctggtc
     3481 gacgcgccct cgaccaacga cgccgtcggc cagctgcggc aggccggtta ttttccgacg
     3541 agtgttttcg aaaatgggcc ggatggcgtg gcggaaaaga aagatcggcg ggcgacgaag
     3601 accaccgcgg cggcgccgag ggccaagggg aaagggatcg ttctctttgc gaagaagacg
     3661 gtcaaagcaa gagtgctgat ggtttttacc cgacaactcg cgaccctgat cgacgctggt
     3721 ctgccgttgc tgcgcggcct caatgtgctc gcgaaacagg agcgcgacct cctgctcaag
     3781 agtaccattc atctactggc cgactcggtc caggggggca gcaccttctc ggaaagcctc
     3841 gcgcagcacc cgaggatttt taacaagctc tttgttaaca tggtgaaagc gggcgagctc
     3901 ggcggtgtgc tagagctcgt gctcacccgg ctcgctgagt tccaggaaaa agcgcagaag
     3961 gtcaaaaata aggtcaaatc cgcgatgatc tatccggcca tcgtgctctc gctcgcgatg
     4021 gtcatcatgg ccttcctcct cgtcttcatc gtgccgaagt tcgaatcgat tttccacgat
     4081 atgctcggca acaagccgct cccggcgatc acccagtttg ttatcgggat cagcggtttc
     4141 atgcaacacc actggctgat catcctcggg gccatcttcg tcatcctcgc cggttataaa
     4201 ttcatctcgc gcacacccgc tggacgggcc gtcatcgacc gactcgcgtt gcgcgcgccg
     4261 cttttcggcg acttgacccg gaagagctcg atctcgcgct tcgcgcgcac ccttggtacc
     4321 ctcgtgacga gcggggttcc gattttggag gcgctcaaca ttacgcgcga gacggccggg
     4381 aacgccgtca tcgccaatgc catttcgcag gtgcaccact cggtcaaaga aggcgaatcc
     4441 atcgtccaac cattggagtc gagcggcgct tttccgccga tggtcatcag catggtcgat
     4501 gtcggcgagg agaccggcca gttgccggag atgctgctca agatcgcgga agtttacgac
     4561 gatgaagtcg acaacgcggt ggcgggcctg acctccatgc tcgagccaat catgattgtg
     4621 ctcctggcgg taattgtcgg cacgatcgtg attgcgctgt tcatgccctt gatcagcatc
     4681 atcggcggcc tgcaggcgca aggctagcgg agggaacaac gaaggccgac tgacgaatgc
     4741 cgaaggaatg ttgaatgacc aatggtgatc ggcctcgcac tggcattcga gaactcggcg
     4801 ctgactcgtc attcgtgatt cgacattcgt cattccgcgc ctgccgcggt ttcacggtca
     4861 tcgagctatt ggtcgtgatg gcgatcactc tcgtcctggc cggcctcgtc ctgacgacga
     4921 gcagttacgt gcataacaag ggcgcgcgtt cccgcgccga agccgagata gccgcgatgt
     4981 cggccgcgct cgagaattac aaggcggata atggggttta tccgatcagc ggcgcaacca
     5041 gtgcactaaa tgcacgaaca tttaccgacc cacgcgacta tcaatctgcg agcgcggctt
     5101 tgtatcaatc actcgcaggc gattccgata ccgacgggct accgggacca ggcgtgtctt
     5161 atatggcatt caagcctaac caactttcgg gcagcggatc gacgattcat atcaaggatc
     5221 cctttggcaa cagctacggt tattcaacag ccggacaagc cggaaccagc ggagttggct
     5281 acaaccccac tttcgatctc tggagcacag ctggctcatt cactgactcc ggtaagtcgg
     5341 gttggatcaa gaattggtaa cgcgaggccg tcttcctgaa cgagctgcag cggagtcgaa
     5401 tgatcccatg gagtgaccgt gcacctatcg ccgcgggatc cctcgactgc gctcgggatg
     5461 acgattgcgg tttgctctca gtgcaccacc accacgaccc gccgattgat ttcttgttcg
     5521 ggaatcgagg catccggcga ggtcagcaaa ttggctttgc cataaccgcg tgtctcgatc
     5581 tgctcgggat tgatcccgag cgcttcgacc aaatacgcct tcacactttc cgcgcgtcgc
     5641 cggcttaaat cgagattgta ttcgtccgac ccatacgagt cggtgtaacc ctcgagcgta
     5701 aaggtcgcgt tcgggttgcg cttgatcagc gtcgcgacct tctgcaattg ctccaccgcg
     5761 ctaccctgca actcggcgct gtcatactcg aaaagctgat tgttaggcat tttgaatttc
     5821 gtccccgctc cgagcggacc cttttgcgag agaagcgatt ccaaatcgct gaaacccggc
     5881 gcgcgatcgg ttcgcggccc ggcgctgtcg cccgggaggc cgttaaacgt attcggtcgc
     5941 ttgattgtcg tgctcgtcgc cagctccgtg cccgtcatcg cgggctgcgg acggccggag
     6001 tcggcgacat cattggacgg cgcctgctgg cgctgcgcgt cgggtgtctt ctcgagcgac
     6061 tgcgccgtcg tgcgctcgat ttcattgatc acggaattca ggttcgtctg ctcgacctgc
     6121 ggtttgtctg gcaggacatc gcgggtgtcg tccggcaggg ccgcggtcgc gtgcacctcc
     6181 tgcaggagtt tgtcgaatga cttctggtca tccggcagtt gcacgtcggt ctggtccgga
     6241 ttcggtttcg cggcctcggc cgcatcgctg gacgatgcct ggtcgagcgg cttgggcgcg
     6301 acctcgacgc tcttcacctt gaatgtgggc ggcgggggtt tttccatccc gagcggcgcc
     6361 gttggttgaa agccggtgcg atagaaaaaa aagcaaagca gtccatgcag ggtaagcgag
     6421 aggagcaggc cgacgagaag ccagttgcgc tgcttgctcc ggccagacca gaccgggaga
     6481 ggcgcgacgg ggcgtgcagt gaaggcgttc atcccgggtt agacaaacga gcgccgaaaa
     6541 tgtttgtcaa actttcggcc cgccctccgg aagcggccga ctttggtggg gcgagacgct
     6601 gtcgagcctg agggaaacct ttgccgcggc ctgccgcacg cccccggctc gaaggagtct
     6661 cgccccaccc gggattgcac gggttcacct aacgaatgga gccgcgcgtt cgttcgatga
     6721 aaatgcgcgc ggcacggcga atctcctccg ccacctgcgc ggccggcgaa acgaatttcg
     6781 gtgtaaacca cggatacaat ccgatcaaat cgtgcgtcac gaggatctgt ccgtcgcaat
     6841 gctcgcccga gccgatcccg atcgtcggga tgccaatcgc ccccgtgatc tggcgggccg
     6901 cttcgctggc gacgatctcg agcacgacgg aaaaagcgcc tgcttcctcg accgcgcgcg
     6961 cgtcggcgag caagcgctcc gcttcggcgg cgccgcgccc tttcaccttg tagccccctt
     7021 ccgcgcgcac gctctggggc agcatgccga tgtgcgccat gagcggaatt cccgccccga
     7081 caatcgctcg gatctgtggg acgtgactgg cgcccccttc cagcttgacc gcgtgcgcgc
     7141 cggcctccac caactggcgg gcattcgcga ccgccgcctc cggggtgtcg tagctgccga
     7201 tggaaaggtc ggcaacgagc agcgctcgct tcaccccgcg cgcggctgct cgagtgtggt
     7261 ggagcatctc ctcgaacttc acctcggtcg tgtccgtgta accgaggacg accatgccta
     7321 acgaatcgcc gacgagaatg atgtcgatcc cactttcgtc cagcaggcga gcggttggat
     7381 aatcgtaggc cgtcagcgcg gtaatgcgtt cccggcggcg ttttcgctcc cgaaaatcct
     7441 ccatcgctgg cgacactacc attgccgggc ggaacgcacc actgatggcc cgtgggtcaa
     7501 ttcctcgctg agttcgccca ccgtccgggc gtgccccggc agcacgagat tggggcgaat
     7561 ttcagccagc ggacgaatca caaacccgcg cagctgcaaa cgggggtggg gcagttgcag
     7621 cagcggttcg tcgatgacgc gcgcgccgtg ataaagcaga tcaaggtcga tcgtgcgcga
     7681 gtggttgcgg gcgtgctccg ccgggcgccc gagagcggct tcaatcttgt tcagctcttc
     7741 cagcagccgg gaggaagctc ccgtgtagcc aatctcgacc actgcgttga gaaacttctt
     7801 tgccccgggc tcacattcga ccggttccgt ttcgtaaagg gcggaggaga gaagcggcgg
     7861 cgatacctcc gggagggcga agatacgggc ccgcgcgttc gttaggcaag aaagccggtc
     7921 gccgagattg gagccgagcg cgatcccggc tctcatcggt agcgccgggt gtgttgcccg
     7981 cagtctaccg tcagggggcc ggcacagccg cctctacaac ccgatgacat tttccatgtt
     8041 gtagagcccg ggcggttgct tgagcgccca gcgcacggcg cgcaacgcgc ccacggcgaa
     8101 agtctcgcgg ctcgacgcgc gatgcgtcag ctccaaacgt tcccccgctc ccgcgaaaat
     8161 gacggtgtgc tcgcccacga tgtcaccggc acgaatggag tgcgccggga cggcgggatg
     8221 gccgccgcgc atttcctgca aaatttccaa aaggcgtttc gcggtcccgc tcggagcgtc
     8281 ctttttcgtg cggtgatgca gctccaccac ctcgagatcg aaatcgtcgc ctaacaactc
     8341 cgctgcgcgc cgggtgagcg cgaagaggac gttgactccg acactgaagt tcgcggcgaa
     8401 aacgagcggg attttctgcg ctaccgccgc cacggcctcc ttttgcgcgg cactatggcc
     8461 ggttgtcccg agaacgagcg gcttcccgtg ctgcgcgcag gcctggcaga cggcttccgt
     8521 tgcatcggcg tcgctgaaat cgatcaccgc gtcgcagttt tcgatcaccc gcgcgacggg
     8581 atcgccgcgc tcgattctgg ccgaaatcgt taggctggtg tctttcgctg cggctgcggc
     8641 caccgcctgg cccatccgac cgttggcgcc gacgagcaag atgcgcgtca cactcatccg
     8701 ccagtctttt ccagggcgtc gagcgttttg cgcaaacgtg cactgttcgc ttccgtcatt
     8761 cccacgagcg gcagccggca ttcggccgac atcaccccgc gccaggcgag gatggtctta
     8821 gccggcactg gattgggctc gatgaaaatg tccttgaaga gggcgaagaa tttccggtgc
     8881 agcgtgcgcg cttcttccgc cttgccctcg agggagagtc gcaccatttg actgacttcc
     8941 gccgggaaaa gattggaggc gacgctgacc actccggccg cgccgacggc cagaaaaggc
     9001 agggtcaacg aatcgtcgcc gctcaggacg gtaaacccat ccggcagccg gccgaccagc
     9061 tcgctcactc ggtcgacgct cccgctcgct tccttgatcg cgacgatgtt ggggcagttt
     9121 tccgccagcc taacgactgt atccgaggcg atatcgaccc cgcagcgtcc cggaatattg
     9181 tagagcacga gcttgagcga cgtcgattcg gcgatcgccg cgaaatgccg gaaaagccct
     9241 tcctggctcg gcttgttgta atagggcgcg acgaggagcg ctccgtccac cccgatttgc
     9301 tccgcctccc tggtgtgcgc gatcgcgtcc cgggtcgagt tcgaacccgt gccggcgatg
     9361 acctggcagc gcttgcgcgc cgcctggacc gccaactcaa tgacctgccg gcgttcgcca
     9421 tggctcaggg tgggcgattc gcccgtcgtc ccgaccggca cgatcccggt gatgccggcg
     9481 gcgatttgcg cttcgatgag ccgggcaaag gcttcgtagt cgacctcgtc gttctggaac
     9541 ggagtgacga gtgctgtgta ggtgccgcgg aacatggtga ggcgcgcgaa cggctaggac
     9601 ggttctcgat tcttcacagc tcaatcgtcc cctcgaaaac aaaatcggcc gggccggtca
     9661 aagtgacgtt gctgaattga tcgcccctcc gggtgaaatc aacctgcaag gtgtcgccgc
     9721 cgcgcaccag gatatcgatc gggccggcca cgttttcatt cgccgcaaaa atgagcgcgc
     9781 tcgcgactac cccagtgcca caggcgaggg tctcatcctc cacgccacgt tcgtaagtgc
     9841 ggatcgcgat gccgcgcgga ccgcgccgct cgctgaaatt ggcattagcg ccgcgcggct
     9901 cgaacatcgg gtgatgacgc agagccgagc ctaacgaccg aacgtccacg tcgtcaattt
     9961 tcgctactgg gacgacgacg tgcggcacgc cgctgtcgat gtaatcggcg cggatctttt
    10021 cgcctccgac cgggacttcg aggcccagcc gcatatcctg cggctcgctc atttgcagcg
    10081 tgaccaattc gcctttgagc gtcgcgccga tcactccggc gggagtttca aaggaaagcc
    10141 tccgccgcgg accggcggtg cggtcggcga agcgggcgaa acaacgcgcg ccgttgccac
    10201 acatctcggc ctcgccgccg tcggcattgt aatagcgcat ccggaagtcg gccccattcg
    10261 ttgcgcgctc gaggaggagc acgccatcgg cgcccacgcc gcgatggcgg tcgcagacgc
    10321 gtgcgatctg ctcgcgggtc agcgcgagat cgccagcgcg attgtcgagc aggacgaaat
    10381 cgttgcccgc gccattcatc ttggtgaaac ggagcatgcg aattttggtt aacatactct
    10441 cgtacgaaag ccaactgggg agctggcgca cgcctggcgc cggcggccgg cttttgaggt
    10501 gggcgaaaat ctcgcgtcta gcctaacgaa gtctccggaa cggttgccgc ggcgcttatt
    10561 tcctctattg gtctcgcgtg cgtttcgcca ccgctctcgc tctcgccgcg ctctgctgcg
    10621 cggtggactc ggcgcccgcc cagttgccgc gcagtccgta caacgcactc attctcgagc
    10681 agatcaaaga gatgccggcg ggcggaaagt attccgcatc gtcgactgcg acgacgcgct
    10741 tgcaatcggc ggtgcagttc gcggcaggca aacttttcat ccagccgatc acggcgatgc
    10801 cgagctattg ctccggggcg acctatctcg ttttcctgaa aacgatcgca gcgctccgcg
    10861 atcgcggcgc cctcagtttg gatgacagga cgctcaacca gttgctcatc cgcgggcagc
    10921 gcgatggcgc agggatctgg ggacgctgga acgccaacgg accgggcacg gcgcgacttt
    10981 ttcatgaact cggactcggg cgcaacttcg acgatttcga agccgcgcag ccgggcgact
    11041 tcatgaaaat tttctggtcg ccggaagtcg ggcgggcgga gcacgggcat tccgtgattt
    11101 ttctccgcat ggagaagaag gaggggatcg attacgtgcg gtattggtcg agcaatattc
    11161 cgtcaggcta cggggaacgg agtgtgccgc gcgcaaaaat cgtgcgcgcg attttttccc
    11221 gactcgaaac gccgtctaat ctttcccgcg tcggggaggt gccgaagttg gatccgtatc
    11281 tcgccagcct ggaacgtgtc cgctccagct acgaagaggc gaaagagaaa tgcggaatgt
    11341 gattttcccc taacgacgga cgcttctcgg ccgccacaat cgcgaatggt ctcgcgcttg
    11401 gctggtcggt ggcctggccg ccgaggccga tcaacgccga gatgattcgc cgcgctctgg
    11461 gtctggctct ggcactaggc cttcggcggt catagaccgc cgctccaggc cggatacatt
    11521 cgtcaccggc atgctggatc aggaaaagaa actgacgatt aatgaaattt atctttcagt
    11581 ccagggtgag agtacctggg cggggctgcc ctgcgttttc gtgaggctga ctttctgtga
    11641 tctgcgctgc acctattgcg acacggaata cgccttttac gaggggaaga aacagtcgtt
    11701 aggtgaaatc gtcgctgcag tcaccagcta cgattgcccg ctggtcgaga tcaccggggg
    11761 tgagccgctg ttgcagaaaa acgtcctgtc gttgatgacg ctgctcgctg atgccgggcg
    11821 caccattttg ctcgagacga gtggcgcgca tgatatttcg ggggtcgatc cgcgcgttca
    11881 ccggattatg gatttgaaaa ctcccggcag cggcgaagtc gcacgcaacc tgttctcgaa
    11941 catcgcgcac ctcaccccgc gcgacgaagt gaaattcgtc attggctcgc gcgaagacta
    12001 cgaatggtcg cgcacgcggg tgcacgaaca tcggctggcg gagcgttgcc gggcggtgct
    12061 tttctcgccg attttcggac ggatcgatcc gcgccagatt gtcgagtgga tcctggccga
    12121 ccacctgccg gtccggttcc agctccagat gcacaagttc atctggacgc cgacgcagcg
    12181 cggggtctaa cgccgccggg tcctgacgtt cggcagaaac gcggtcagca gcccgatcag
    12241 cggcaggaag gagcagagct gaaagacgta gttaatgctg gtgcgatcgg ccagctcgcc
    12301 aagcacggcg gagccgatgc cagccatacc gaaggccagc ccgaaaaata atcccg
//
LOCUS       DATMNS010000006         5250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5018907, whole genome shotgun sequence.
ACCESSION   DATMNS010000006 DATMNS010000000
VERSION     DATMNS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5250
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5018907"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VNW28_00165"
     CDS             <1..774
                     /locus_tag="VNW28_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08361.1"
                     /translation="MSTVAVALGMFAALLALLVALRAKTGNKIDIRNSDIVLALVPVA
                     LWLFLTGKIQEFAFGEVKIVAAIKLASAAPVGPEVSKLTIDAVREPVRMMSKGAPDVI
                     PRMINTKSQALSFTIGQGGYAGPAIGDYLDQLTQYPFLRYIVLNNSDGTFFGIADARQ
                     IEDVVRASNPRFNLDAFADWINGGQKAELAALPGFIPAEKALHKKDDKRKALERMNTE
                     DAQVLPVLDEAENFDGIVDRSKLTASILTEIAQRVEKAD"
     gene            complement(1043..2098)
                     /locus_tag="VNW28_00170"
     CDS             complement(1043..2098)
                     /locus_tag="VNW28_00170"
                     /inference="COORDINATES: protein motif:HMM:NF042449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase assembly
                     protein OpcA"
                     /protein_id="HXA08362.1"
                     /translation="MPATAENFTAGMPVEIGQIGRELKKLWDAGDGVMTRASLINLAV
                     YSEAPDSLGTNTALISQITENHACRAIVIAADPTAKESRVEAWISAHCHVSQAGRKQV
                     CSEQLSFFLQGEQCGLLPNIVFAQLDSDLPLYLWWQEEFRAPMDPQLWAWVDRVIYDS
                     QVWRNFEEEMRLVEIAQREARQRIVLCDLNWVRLVHLRIALAQFFDHPATYHHLAEIK
                     SLEIEHAPGFRSTALLLVGWLAGQLGWERASGKSLGFRRLEDDGERKIQVSLREKKGE
                     PIGSCKITTDSAEFHVEHKPGADLLDLNFGRTSRQMQQVLPAGKNDLVGLMKEELMRG
                     GPHRVYLRAVEQLRHLL"
     gene            complement(2209..3747)
                     /gene="zwf"
                     /locus_tag="VNW28_00175"
     CDS             complement(2209..3747)
                     /gene="zwf"
                     /locus_tag="VNW28_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXA08363.1"
                     /translation="MSEKHPANPLREGLSTKAVPQPCAVVIFGATGDLTHRKLVPALY
                     NIAADGELPPAVAVVGFARRPKTDDEFRKEMEEATRKFSRQTVRDEIWDGFARSLYYH
                     QSEFGDEAGYQKLAARLDRLDEESGTRGNRLFYFATSPEQFETIVQNLKRAGLNKARE
                     GSWARVILEKPFGTDLKSARQLNRVVQSSFAEDQTYRIDHFLGKETAQNILVLRFANA
                     IFEPLWNTRYIDHVQITAAETLGVENRAGYYEGAGALRDMVQNHLLQLLCLVGMEPPT
                     DLDADSIRDEKVKVVRSLRRFTEEQVKTQVVRGQYAEGAINGQAVAAYRTENGVDPKS
                     QTETFVALRILVDNWRWADVPIYVRVGKRLPKSGTEISVHFKKAPQVLFNRESSALDQ
                     NVLVIRIQPDEGISLRMQAKVPGTSFRIESVKMDFHYGTSFGKASPEAYERLLLDAMA
                     GDATLFARRDEVERAWAFIDTIEEAWVATEDAPKLCTYPAGSWGPEEADELLARDGRS
                     WRRL"
     gene            complement(3767..>5250)
                     /locus_tag="VNW28_00180"
     CDS             complement(3767..>5250)
                     /locus_tag="VNW28_00180"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HXA08364.1"
                     /translation="FEEPGATLWRDLPDPIGMGREVYEECAESIEKALPSVLAFVEET
                     ELAIPRAARESSATRSTSGNMPSPLESEPGDGHSTASSGSALWKVDPEIFDAIAAEER
                     RQRENIELIASENFTSRAVMEAQGSCLTNKYAEGYPGKRWYGGCENVDVVEQLAIDRA
                     KKIFGADHVNVQPHSGAQANMAVYFAAIKPGDRILTMNLAHGGHLTHGHPANFSGQLY
                     EVTHYGVDQQTEQIDYDALAKQAAEVRPAMITAGASAYPRFLDFPRLRQIADSVDALL
                     FIDMAHIAGLVAGGEHPSPVPHAHFVTTTTHKSMRGPRGGIVLCQAEFAKSIDSLVFP
                     GVQGGPLMHVIAAKAVCFHEALQPGFRVYQRQVVLNAKALAAGVAKHGFRIVSGGTDN
                     HLMLVDLRPKEINGKVAQDVLDRAGITVNKNAIPFDTYPIFKPGGIRLGTPAVTTRGM
                     KEEEMLEIADLIAEGLAARDDERALAQVRQQVQQLTRRFPLPS"
BASE COUNT          965 a         1626 c         1572 g         1087 t
ORIGIN      
        1 atgagcacgg tcgcagttgc gctcggcatg tttgccgcac tcctcgcgtt gctcgtcgcc
       61 ctccgggcga agaccgggaa caagatcgac atccgcaact ccgacatcgt gctcgcattg
      121 gttccggtcg cgctctggct ctttctcacc ggcaagattc aggaattcgc cttcggcgag
      181 gtaaaaatcg tcgccgcaat caagctcgcc tcggccgcgc ccgtggggcc ggaagtttcc
      241 aaactaacga tcgacgcggt gcgcgaaccg gtgcgcatga tgtcgaaggg tgcgccggac
      301 gtgattccgc gaatgatcaa tacgaagagc caggcgctta gttttacgat cggccaggga
      361 ggctacgccg gtccggcgat cggtgattat cttgaccaac tgacgcagta tccctttctc
      421 cgctacatcg tgctcaacaa ttcggatggc actttttttg ggatcgcgga tgcacgccaa
      481 atcgaggatg tggtgcgcgc ttccaatcct cgcttcaacc tggacgcctt cgccgattgg
      541 atcaacggcg gccagaaggc tgaactggcg gcgttgcccg gtttcattcc ggcggagaaa
      601 gcgctgcaca aaaaggacga caaacgcaaa gcgctcgaaa ggatgaacac cgaggatgcc
      661 caggtgctgc ccgtcctcga cgaggcggaa aatttcgatg gcatcgtcga ccgctccaaa
      721 ctcaccgcca gcatcctgac cgagatcgcg cagcgggtgg aaaaggccga ttgagcaagc
      781 cttcgttagg tgccgactaa cgaatcgcgc gccctgtaga cgttgccctg cgggcaactc
      841 gggccagcgc atggccggaa atcgtagcga gcgtttcccc cgaagcttcg caccgcgcag
      901 cgtctagtgc accgtatcag atgtttcgtg agtgagtatg atatacgtga gtcacgtata
      961 tcatcaaatg cacggttgga gcgcaatgtc attgcgacga cagagcgtcg cgctccacgc
     1021 acgtttcaag tgatacaggg cactagagaa ggtgtcgcag ctgctcgacc gcgcgcagat
     1081 aaacccgatg cggtccgccg cgcatcaact cttctttcat cagcccgacg agatcgttct
     1141 ttccggcggg caggacctgc tgcatctgcc ggctggtgcg accaaaatta aggtcgagca
     1201 aatccgcgcc gggtttgtgc tcgacgtgaa actccgcgct gtctgtcgtg atcttgcagc
     1261 tcccgatggg ttcgcctttt ttttcgcgga gcgaaacctg gatcttccgt tcgccgtcat
     1321 cctccagccg gcggaagcct aacgattttc cgctggcccg ttcccagccg agctgccccg
     1381 ccagccagcc gacgaggagc agcgccgtcg aacgaaatcc cggcgcgtgt tcgatttcga
     1441 gcgacttgat ctcggcgagg tgatgatagg tcgcgggatg atcaaaaaat tgagcgagcg
     1501 caatccgcag gtggacgaga cgcacccaat tgaggtcgca gagcacgatg cgttgccgcg
     1561 cctcgcgttg cgcgatctca accaggcgca tctcttcctc aaagttgcgc cagacctggc
     1621 tgtcgtaaat cacgcgatcg acccaggccc aaagttgcgg gtccatcgga gcgcgaaact
     1681 cctcctgcca ccaaagataa agcggcaggt ccgaatcgag ctgtgcgaaa acgatgttcg
     1741 ggagcagccc gcactgttcg ccctggagaa aaaatgaaag ttgctccgaa caaacctgtt
     1801 tgcggcccgc ttggctgacg tgacagtgcg cgctgatcca ggcctcgacc cggctttctt
     1861 tggcggtcgg gtccgcggca atcacaattg cccggcaggc gtggttctcg gtgatctgcg
     1921 aaatcagcgc cgtgttcgtg cctaacgagt cgggggcttc gctgtaaacc gcgagattga
     1981 tgagtgacgc ccgggtcatc accccgtcgc cggcgtccca gagttttttc aactcgcgcc
     2041 cgatctggcc gatctcgacc ggcatgccgg cggtgaagtt ttcagccgtg gcgggcatga
     2101 ttcaggtcgt cccgagcgaa gccgagggat tccgtggcgt caccggaagc ttgcgccccg
     2161 agatccttcg actccgctgc gctccgctca gggtaacgat tatctgcatc ataacctccg
     2221 ccagctccgc ccatcgcgcg ccagcagctc gtccgcttcc tccggtcccc aggagccggc
     2281 cggataggtg cacaatttcg gcgcgtcttc cgtcgcaacc catgcttcct cgatcgtgtc
     2341 gatgaacgcc cacgcccgct ccacctcgtc gcggcgggcg aagagcgtcg cgtcacccgc
     2401 catcgcgtcg aggagcaatc gttcgtaggc ttccgggctc gctttgccga aggatgttcc
     2461 gtaatgaaaa tccatcttca cggattcaat ccggaagctc gtgccgggaa ctttcgcctg
     2521 catccggagc gagattcctt cgtccggctg gatgcgaatc acgagcacgt tctggtcgag
     2581 cgcgctcgat tcgcgattga acagcacctg cggcgctttc ttgaaatgca ccgaaatctc
     2641 ggtcccggat ttgggcagcc gtttgccgac ccggacgtag atcggcacgt ccgcccagcg
     2701 ccagttatcg accaggatgc ggagggcaac aaaggtctcg gtctgtgact tcgggtcgac
     2761 cccattttcc gtgcggtagg cggcgaccgc ctgaccgttg atcgcccctt ccgcgtactg
     2821 accgcggacc acctgcgttt tgacttgctc ttccgtgaag cggcggagtg agcggacgac
     2881 ttttactttt tcatcgcgaa tactgtccgc gtccaggtcc gtgggcggct ccatcccgac
     2941 gaggcaaagt aattgcagga ggtggttctg caccatgtcg cgcaaagccc cggcgccttc
     3001 gtagtaaccg gcgcggtttt ccacgcctaa cgtttccgcg gcggtgatct ggacatgatc
     3061 gatgtagcgc gtgttccaga gcggctcgaa aatcgcgttc gcgaaacgca ggaccaaaat
     3121 attctgcgcg gtttccttgc cgaggaagtg atcgatccgg taggtctggt cttcggcgaa
     3181 ggagctctgc acgactcgat tcaattgccg cgcggatttg agatcggtcc caaacggctt
     3241 ctccagaatc acccgcgccc agcttccttc gcgcgccttg ttcagtccgg cccttttcag
     3301 gttttgcacg atcgtttcga actgttcggg cgaagtcgcg aagtagaaga ggcggttgcc
     3361 gcgcgtgcca ctttcttcgt ccagccggtc gaggcgcgcg gccagttttt gatagccggc
     3421 ttcatcgcca aattcgctct gatgataata aagcgacctg gcaaaaccgt cccagatttc
     3481 gtcgcgcacg gtttgccgcg aaaacttgcg agtcgcttct tccatttcct tccgaaactc
     3541 gtcatcggtc ttcggccggc gcgcgaaacc gacaaccgcg acggccggcg ggagctcgcc
     3601 gtccgcagcg atgttgtaga gtgcggggac gagtttgcga tgggtgaggt cgccggttgc
     3661 gccaaaaatc accacggcgc aaggctgcgg cacagccttg gtcgagagcc cttcgcgcag
     3721 gggatttgct ggatgctttt cgctcatgtt tttcgtgctc tcggcgtcag ctcggcaacg
     3781 gaaaccggcg cgtcaattgt tgaacctgct gacgcacctg cgcgagcgcg cgctcgtcgt
     3841 cgcgcgccgc caggccttcg gcgatcaggt cggcgatttc gagcatctct tcctctttca
     3901 ttccgcgggt cgtgaccgcc ggcgtgccga ggcgaatgcc gccgggcttg aagatcggat
     3961 aggtgtcgaa cggaatcgcg ttcttgttca ccgtgattcc cgcgcgatcg agcacgtcct
     4021 gcgcgacttt tccgttaatt tccttcggcc gcagatcgac cagcatgaga tgattatcgg
     4081 ttccgccgga aacgatccgg aaaccatgct tcgcgacgcc ggcggcgagc gccttggcgt
     4141 tcaacaccac ctgccgctgg tagacgcgga agcccggctg cagcgcctcg tgaaaacaga
     4201 cggccttggc cgcgatcacg tgcatgagcg gcccgccctg gacgccgggg aagactaacg
     4261 agtcgatcga tttcgcaaat tctgcctggc aaaggacaat cccgccgcgc ggcccgcgca
     4321 tgctcttgtg cgtcgtggtc gtgacgaaat gcgcatgcgg caccgggctg ggatgttcgc
     4381 cgccggcgac caggcccgcg atgtgcgcca tgtcgataaa caaaagcgca tcgaccgaat
     4441 ccgcgatctg ccgcaggcgc gggaaatcga ggaagcgcgg ataagccgaa gcccccgccg
     4501 tgatcatcgc ggggcgcact tccgcggcct gtttggcgag cgcgtcgtaa tcgatctgct
     4561 ccgtctgttg atcgacgccg tagtgcgtca cttcgtaaag ctggccggaa aaattcgccg
     4621 gatggccgtg agtcaagtgg ccgccatggg cgaggttcat cgtcaggatg cgatcgcccg
     4681 gtttgatcgc ggcaaagtag accgccatgt tggcctgcgc gccgctgtgc ggttgcacgt
     4741 tgacgtggtc cgccccgaag atttttttcg cccgatcgat cgccagttgt tcgacgacgt
     4801 cgacattctc acaaccgccg taccagcgtt tgccgggata accctcggca tatttgttcg
     4861 tcaggcacga accctgggcc tccatcacgg cgcggctcgt aaaattttcc gaggcgatca
     4921 actcgatgtt ttcgcgctgt cgcctttcct cggcggcgat ggcgtcaaaa atttcgggat
     4981 ccactttcca cagcgcgctg cctgacgacg cggtgctgtg gccgtcgccg ggttcggatt
     5041 cgagaggtga aggcatattc ccgctggtgg atcgcgtggc ggaactctcg cgggcggccc
     5101 gcgggatcgc cagttcggtt tcttctacga aggcgagcac gctcggtaaa gccttttcaa
     5161 tgctctcggc gcattcctcg taaacctcgc gccccatccc gatcggatcg ggcagatccc
     5221 gccagagggt ggcgcccggt tcttcaaact
//
LOCUS       DATMNS010000007         6775 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_369476, whole genome shotgun sequence.
ACCESSION   DATMNS010000007 DATMNS010000000
VERSION     DATMNS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6775
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369476"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VNW28_00185"
     CDS             complement(<1..718)
                     /locus_tag="VNW28_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXA08365.1"
                     /translation="MAKSGLGKGLGALISVAPSLPATEPEAGERVQLVGLASIVPSPL
                     QPRREFGREALEELVDSIRQRGIIQPLIVRPVNRQFELIAGERRWRAAKEVGLTEAPV
                     IVRQATDLEVLELSLIENLQRSDLNPIEEAQAYARLAGEFGMRQDDIAQKVGKSRASV
                     ANAMRLLDLDQQVQTWVVQNLLSVGHAKVLLGLKAHDEQRLLAEKILRQSSTVRTTER
                     MVARQLGRLGGARRRKRADTV"
     gene            884..1759
                     /locus_tag="VNW28_00190"
     CDS             884..1759
                     /locus_tag="VNW28_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HXA08366.1"
                     /translation="MLARDARVFTLGHSPDPDDAFMFYAMAEHKIDLRGYQFEHRLED
                     IQTLNERALRGELDISAISIHAYAYVSDRYALLPSGASMGDGYGPMLVAADPGAPTSG
                     APNELYEWLRGRLVAVPGKMTSAYLALQLFLGEFESVVVPFDQIFATVKSGRAATGLI
                     IHEGQLTYAAAGFDKLVDLGAWWKAKTSLPLPLGGNVLRKHLPPQVRRDLSQIIRESI
                     DYGLAHRAAAVRHSLPYAREMDAELASKFIGMYVNDFTRDYGETGRKAIREFLRAGSE
                     RGYVPATSEVEFVEE"
     gene            complement(1814..1999)
                     /locus_tag="VNW28_00195"
     CDS             complement(1814..1999)
                     /locus_tag="VNW28_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08367.1"
                     /translation="MGLIRSLFWFVLFVLFTFSFVVLFEYGTTDFVSGFQKEFQRVTK
                     FVEEAAHPKKKKKDTTP"
     gene            2208..3407
                     /locus_tag="VNW28_00200"
     CDS             2208..3407
                     /locus_tag="VNW28_00200"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXA08368.1"
                     /translation="MRVPLLDLSEQYRVLAEPIRRELDDILRTQTFILGPKVEEFERA
                     LAEYCGVKHAIGVSSGTDALLAILMALGIGLGDAVVTTAYTFFATGGCIARVGATPIF
                     VEIDPATFNISPTAIANFLANHCQRNARNELVDSVGRVVRAIIPVHLFGLCCEMDAIN
                     ELAQHSRLTVIEDAAQAIGAEYPAKQGAARQAGTMSAVGCFSFYPSKNLGAAGDAGMI
                     VCDDDELAGRLRSFRQHGMEPRYFHHVIGGNFRIDAMQAAILKVKLPYLNGWSAARRA
                     VADIYRDEFTRRGPTNRVTLPAEPYRDSGLTNHHIYHQYVIRTPQRDALQSHLAQRGI
                     GSAIYYPLGLHEQKCFAHLGYRAGDLPETERAARETLALPIYPELTGAMQAEVVGAIA
                     EFFADKS"
     gene            3415..4026
                     /locus_tag="VNW28_00205"
     CDS             3415..4026
                     /locus_tag="VNW28_00205"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXA08369.1"
                     /translation="MNGALDFVHQFIPGSSERTLLLLHGTGGNEHQLIPLGRELDPTA
                     ALLSPRGKVLENGYARFFRRLAEGVFDLEDLQRRTDELAEFVLAAAAHYQFAVEKVAA
                     VGYSNGANIAASILLRRPGLLQTAVLLRAMVPFLPEKLPDLSTVRVWIGGGKLDAIIA
                     PTETERLVDLLQAAGAKVSAHFFDAGHGLTKEEFVLVSRWLAL"
     gene            complement(4076..5050)
                     /locus_tag="VNW28_00210"
     CDS             complement(4076..5050)
                     /locus_tag="VNW28_00210"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HXA08370.1"
                     /translation="MNTPPALSTLSEADREQFWPPLPFAAWQATAETLHLWTQIVGKI
                     RLALTPWINHSWHVTLYLTARGLTTSPIPYRFHTFEIRFDFISHELRVLKSDGGLRVL
                     PLRAQSVATFYHAVLAALRELDLPVRIDLTPNEITDPIPFERDETHCAYDPAQANRFW
                     RVLRQSDRVLQKFRSRFCGKCSPVHFFWGSFDLAVTRFSGRAAPPHPGGIPHLPDTVT
                     REAYAQEVSSVGFWPGSAASPTPLYYSYAYPEPPGFAEAQVKPAEASYYAPLREFILP
                     YDAVRQAASPDEALLAFAQSVYDAASILGKWDRAALEEAGLKPPASLP"
     gene            5129..5214
                     /locus_tag="VNW28_00215"
     tRNA            5129..5214
                     /locus_tag="VNW28_00215"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5163..5165,aa:Tyr,seq:gta)
     gene            complement(5459..5878)
                     /locus_tag="VNW28_00220"
     CDS             complement(5459..5878)
                     /locus_tag="VNW28_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08371.1"
                     /translation="MNAALKWKLVAGFVLAFLAGGATGGFLVASQARHRQTDFAQHRH
                     SLAERMRGQLQVDLNLTPEQIQKTAPIFDRAVNELEKIRAETGARVREVMAETRQALA
                     PELSDAQRTKLEALTTHPRLNRGPQNPPRHRESPPPN"
     gene            complement(5875..6183)
                     /locus_tag="VNW28_00225"
     CDS             complement(5875..6183)
                     /locus_tag="VNW28_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08372.1"
                     /translation="MKSSDRQLERLLHAAAEATSEAPNEMPFGFDTRVLAHWRAGLNR
                     DFVDVGHLLRRVVLLSLAVIALAGAGTYHELRQSDEFGELLADEYGIADSAIGNVFEQ
                     "
     gene            complement(6180..>6775)
                     /locus_tag="VNW28_00230"
     CDS             complement(6180..>6775)
                     /locus_tag="VNW28_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXA08373.1"
                     /translation="LDDMADEFDAGALVAAARQHDENAAREMVRRLYPLVVKLVRSHR
                     PRRAAEEDLCQMIFIKIFQNLNQFSGKVPLEHWVSRIAINTCINQIQAEKVRPELRQA
                     DLSEEELAVVQNLAATPEELGPDRSFAARDLVERLLAALKPAERLVIDLLYLRQHSIA
                     EIQALTGWGASLVKVRAFRARQKLKRQLAKFSEEGLR"
BASE COUNT         1254 a         2112 c         2152 g         1257 t
ORIGIN      
        1 tgacggtgtc ggcgcgcttg cgccgccgtg cccccccgag cctgccgagc tggcgcgcga
       61 ccatgcgctc ggtcgtgcgg acggtggagc tttgccgtaa aattttctcg gcgaggagcc
      121 gttgttcatc gtgcgctttc aagccgagca acactttggc gtggcccacc gagagaagat
      181 tctgcaccac ccaggtctgc acctgctggt cgagatcgag caaccgcatg gcgttcgcga
      241 ccgaggcgcg gctcttcccg actttctgtg cgatgtcgtc ctggcgcatg ccaaattcac
      301 cggcgagccg ggcataagcc tgcgcttcct cgatcgggtt caggtcggag cgctggagat
      361 tttcgatcag ggaaagctcg agcacctcaa gatcggtcgc ttgcctaacg atcaccggcg
      421 cctcggtcag gccgacttcc ttggccgcgc gccagcggcg ctcgcccgcg atgagctcaa
      481 actggcgatt gaccggccta acgataaggg gctggataat gccgcgttgc cggatcgaat
      541 cgaccagctc ctcgagcgcc tcgcgcccga attcccggcg cggctggagc gggctcggca
      601 cgatactggc gaggccgacc agttgcactc tttcgccggc ttccggctcg gttgctggca
      661 gggaaggggc gacgctgatc agtgcgccga gacctttacc gagaccggat tttgccatcg
      721 cgggctcagg ctatcggaag caccgtcgca aagcaactgt cgcccggggg cgtcgatccc
      781 ggcagtagct cagccgtgag accacttccg cacctgggtc gacgccccgg gcgacaaccg
      841 cttcgattgc accacgcaac gcccccgttt agcatcaccc gccatgctgg cccgcgacgc
      901 gcgcgttttc actctgggac attcccccga tccggatgac gccttcatgt tttacgccat
      961 ggcggagcac aagatcgacc tgcgcggcta ccaattcgag caccggctcg aggacatcca
     1021 gacgctgaac gagcgcgcgt tgcgcggcga actcgatatc tcggccatct ccattcacgc
     1081 ctacgcctat gtgagcgatc ggtatgcact tctcccgtcc ggcgcgagca tgggggacgg
     1141 ctacggcccg atgctggtgg cggcggaccc gggcgcgcca acgagcggtg cgcctaacga
     1201 actctacgag tggctgcgcg gccgcctcgt cgccgtgcct ggcaagatga cgagtgctta
     1261 cctggccctg cagctttttc tcggcgagtt cgaatccgtg gtcgtgccgt tcgatcagat
     1321 cttcgcgacc gtcaaatcgg ggcgcgcggc gaccggtctg atcattcacg aaggccagct
     1381 cacctatgcg gcggccggtt tcgacaagct ggtcgatctc ggcgcatggt ggaaagcgaa
     1441 gacgagcctg ccgttgcccc tgggcggcaa tgtcctgcgc aagcatctcc cgccgcaagt
     1501 ccgccgcgac ctttcccaaa tcatccggga aagcatcgac tacggattgg ctcatcgcgc
     1561 tgccgcggtg cgccactcgc tgccctatgc ccgcgagatg gacgcggagc tcgcaagcaa
     1621 attcatcggc atgtacgtga acgattttac gcgtgattac ggtgagaccg ggcgcaaggc
     1681 gattcgggaa ttcctccgtg ctgggagcga gcgcggttat gtgccggcga ccagcgaagt
     1741 ggaatttgtc gaggaatgac caactccgag tgacgaagga aaaacgcgcc gccttcgtcc
     1801 ttccagtcga aggctagggc gtggtgtcct tcttcttctt cttggggtgg gcggcttcct
     1861 ccacgaattt cgtcacgcgc tgaaattcct tttgaaagcc ggaaacgaag tcggtcgtcc
     1921 cgtattcaaa aaggacgaca aagctgaaag tgaagagcac aaacagcacg aaccaaaaca
     1981 aactccggat gagacccacg cgcggagagt agcttaccgg ctctctgcgg tcaatgaccc
     2041 gagcacaatc ctccgcgcgc gagtcctttg atgattgttc gtcgcggtcc cgcagcgccc
     2101 tggagccggg tcagggtcgc gatgtcattg agcgaaaccg gccggtccga gaatcgacga
     2161 acccacctcg ccctcgccag agcccgtccc tcatgcttcg gtagcccatg cgcgtgccgt
     2221 tgctcgatct cagcgaacaa taccgggtgc tggccgagcc gatccgaagg gaattggacg
     2281 acatcctccg gacacaaact tttatcctcg ggccgaaggt ggaggaattc gagcgcgcgc
     2341 tcgcggaata ctgcggcgtg aaacatgcca tcggcgtctc ttcggggacg gacgcgctgc
     2401 tcgccatcct gatggcgctc gggattggcc tcggcgacgc ggtagtcacg accgcctaca
     2461 catttttcgc gactggcggc tgcatcgccc gggtcggcgc aacgccgatc tttgtcgaga
     2521 tcgatccggc gactttcaac atctcgccaa cagccatcgc gaatttcctg gccaaccact
     2581 gccagagaaa cgcgcggaac gaactcgtcg attccgtggg acgcgtcgtg agggcgatca
     2641 tcccggtgca cctctttggt ttgtgctgtg agatggatgc gatcaacgaa ctcgcccagc
     2701 actcccgcct cacggtgatc gaagacgcgg cacaggcgat tggcgcggag tatccggcga
     2761 agcagggcgc ggcgcgacag gccggcacga tgagcgcagt tggctgtttt agcttttatc
     2821 cttcaaagaa tctcggcgcc gccggggacg cggggatgat cgtttgcgac gatgacgaac
     2881 ttgcagggcg cttgcgcagt tttcgccaac atgggatgga gccgcgctat ttccatcacg
     2941 tcatcggcgg caattttcgg atcgacgcga tgcaagccgc gatcttgaag gttaagctgc
     3001 catacctcaa cggctggtcc gcggcacgcc gggcagtggc cgacatctac cgtgacgaat
     3061 tcactcggcg cggcccaacg aaccgcgtga cgctgccggc cgagccctac cgggactctg
     3121 gcctgacgaa tcatcacatt tatcatcagt acgtcatccg cactccacag cgcgacgcgc
     3181 tccaaagcca cctcgcgcag cgcggaatcg ggtccgccat ttactatccg ctcggattgc
     3241 acgagcagaa atgcttcgcc cacctcggtt atcgtgccgg cgatttgccg gaaaccgaac
     3301 gggccgcgcg cgaaacgctg gcgctgccca tctatccgga gctgacgggc gccatgcagg
     3361 ccgaagtcgt gggtgcgatt gctgaatttt ttgcggacaa gagttgatcg accaatgaac
     3421 ggcgcactcg attttgtcca ccaattcatc cccgggtctt cggagcggac cctgctccta
     3481 ttgcatggca ccgggggaaa tgagcaccag ctcatcccgc tcggccgcga gctcgatccg
     3541 acggcggcct tgcttagtcc gcggggcaag gttttggaga acggctacgc gcgattcttt
     3601 cgccggctgg cggaaggggt gtttgacctc gaggatttgc agcgacgcac ggacgagctg
     3661 gcggagttcg ttctggccgc ggccgcccat tatcagttcg cggtcgagaa agtggcggcg
     3721 gtcggttatt ccaacggcgc gaacatcgcg gcgagcatcc tgttgcgccg gccgggcctc
     3781 ctgcaaaccg cggtcctgtt gcgcgccatg gtgccattcc tgcccgaaaa actaccggat
     3841 ctttccacgg tgcgggtctg gatcggcggc ggaaaactgg atgcgatcat tgcgccgacc
     3901 gagacggagc ggctggtgga ccttctgcag gcggcgggag cgaaggtgag cgcgcatttc
     3961 ttcgacgcgg gtcacggcct aacgaaggag gaattcgtct tggtgagccg ctggctggcc
     4021 ctgtagcctt ggggcgccga cccccggcca ccccacgccc ggcgacaact gcgcttcacg
     4081 gcaggctcgc cggcggtttg agccccgctt cctccaacgc ggcgcgatcc cacttcccga
     4141 gaatcgaagc ggcgtcgtag acgctttgcg caaaggcgag cagcgcctca tcgggcgaag
     4201 cagcctggcg cacggcgtcg tagggcagaa tgaattcgcg caacggtgcg taatagctcg
     4261 cctccgccgg ttttacctgt gcctcggcaa accccggcgg ctcgggatag gcataggaat
     4321 aatagagcgg cgtggggctg gccgcgctgc cgggccagaa gccaacgctg cttacttcct
     4381 gggcgtaggc ttcgcgcgtc accgtatcag gcaaatgcgg gatgcccccc ggatgcggcg
     4441 gcgcggcgcg gccggaaaag cgcgtgaccg ccagatcgaa actgccccag aaaaaatgga
     4501 ccgggctgca cttgccgcag aagcgggaac gaaatttttg cagaacgcga tccgattgcc
     4561 gaagaacgcg ccagaatcgg ttcgcctgcg ccggatcgta cgcgcaatgc gtttcgtcgc
     4621 gctcaaacgg aatcggatcg gtgatctcgt taggcgtgag gtcgattctg accggcaaat
     4681 cgagctctct cagcgccgcc agcaccgcgt gataaaaagt cgcgaccgac tgagcccgga
     4741 gcgggaggac ccggaggccc ccgtcacttt tcaaaacgcg caattcatga ctgatgaaat
     4801 cgaaccggat ctcgaaagta tggaagcggt acgggatggg cgaagtcgtc aggccgcgcg
     4861 ccgtgagata aagcgtgacg tgccaggaat ggttgatcca cggagtgagc gcgaggcgga
     4921 tcttgcccac gatctgtgtc cataggtgga gcgtctcggc ggtcgcctgc catgcggcga
     4981 aagggagcgg cggccagaat tgttcgcgat cagcctcgga aagagtcgaa agagcaggcg
     5041 gtgtgttcat ggttgtgccg gaatctacct caccggcaaa acagttgcga ccgccagcga
     5101 gcgagcctat atcagacgct cgtcgcgcgg gtaggtaccg aagtggtcaa acggggcgga
     5161 ctgtaaatcc gctggcttac gccttcgctg gttcgaatcc agccctgccc accaccgttc
     5221 cgcttacgcg gtcgaggccg cgatcggcct aactgttagg gcagcgattt ggagtcagag
     5281 accggaagcg gacgcttcgt ggtccgggaa tgcgcgccgt agagcgctag tggcgcgtat
     5341 ccgtcatttg tatcgcaatg aactttgcgt ttgatatacg tgagtcacgt atatgctcgg
     5401 cgacggacac ggcgcgaaaa gaaggaacgg ctgctatatg agcaagtgat gcagggcact
     5461 agttcggcgg cggcgactcg cgatgtcggg gcggattctg cggcccgcgg ttcagtcgcg
     5521 gatgcgttgt caaagcctcg agctttgtcc gctgcgcgtc gctcagctcc ggcgccaacg
     5581 cctgcctggt ctcggccatg acttcgcgca cgcgcgcgcc ggtttccgcg cggatcttct
     5641 ccagttcgtt caccgcccgg tcgaagatgg gcgcggtctt ctggatctgc tcgggcgtga
     5701 gattgaggtc gacttgcagc tggccgcgca tccgctcggc taacgagtga cggtgttgcg
     5761 caaaatctgt ctggcgatgg cgcgcctggc tggccacgag gaagccaccg gtggcgccgc
     5821 cggcgagaaa ggccaggacg aagccggcca ctagtttcca tttgagcgcg gcgttcattg
     5881 ctcgaagacg ttcccgatcg cagaatcggc gatcccatac tcatcggcga gaagctcgcc
     5941 gaactcgtcg ctttggcgca gctcgtggta ggtgccggcg cccgccagcg cgatcacggc
     6001 caacgagagc aggacaaccc ggcgcaggag gtgcccgacg tcgacgaaat cgcggttcag
     6061 gcccgcccgc cagtgcgcca gcacgcgggt gtcgaaacca aacggcattt cgttcggcgc
     6121 ctcgctcgtc gcctccgcgg cggcgtggag taaccgctcc agttgccggt cggaactttt
     6181 catcgcaacc cttcctcgga aaattttgcg agctgccgtt tcaacttctg gcgggcgcgg
     6241 aaggcgcgca ctttgaccag cgatgcgccc cacccggtca acgcctggat ttccgcgatg
     6301 gaatgctggc gcaggtaaag gagatcgatc accaaacgct ccgccggctt aagcgccgcg
     6361 agcaggcgtt cgaccaggtc gcgcgcggcg aaactgcggt ccgggcccaa ttcttccggg
     6421 gtagcggcga ggttctgcac gacggcgagt tcttcttcgc tcaagtccgc ctggcgcaat
     6481 tccggccgca ctttctcggc ctggatctgg ttgatacaag tgttgattgc gatccgcgag
     6541 acccagtgct cgagggggac cttgccggag aactgattca gattttggaa aattttaatg
     6601 aaaatcatct gacaaagatc ctcctcggcg gcgcggcgcg gccggtggga gcgcaccagt
     6661 ttgaccacga gcgggtagag ccggcgcacc atctcgcgcg cggcattttc atcgtgctga
     6721 cgcgccgcgg ccaccagcgc cccggcgtcg aattcatcgg ccatgtcatc caagc
//
LOCUS       DATMNS010000008         8115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4301771, whole genome shotgun sequence.
ACCESSION   DATMNS010000008 DATMNS010000000
VERSION     DATMNS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4301771"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VNW28_00235"
     CDS             complement(<1..1466)
                     /locus_tag="VNW28_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXA08374.1"
                     /translation="MNDFSLLETEIGHGLLLIEASAGTGKTFTIAGLVLRLLLERPGL
                     TIDKILVTTFTELATAELRGRIRDLLRDGLGAFQAGKSDHDLIGAILQKYPDHRAAIQ
                     RLKDALANFDEAPIYTIHGFCQRVLAEQAFETGTLFDAELVTDERDLLREVTWDFWRR
                     NFYSNDSLGVLVAIQGKITPKKLLKDLEELVKNPTLTILPKDLRSSAEAARQVEHALK
                     EVRNVWPECASGIRAFFANPNWAKGDYKKAELLARVLEDLEQVASGKGASARQFESIE
                     LLAAEPIAKGVRKGFSLPKDPIFGTCQIAVDRSADFCTALRAEFHAWAPNELRRRKQA
                     QNVLAFEDLLTRLDDALRGAGGSALAESIRQKYRAALVDEFQDTDPVQYSIFERIYQG
                     SDATVALIGDPKQAIYAFRGADVFTYLNAARAIRRQFTLRTNWRSESGLVRGTNTVFD
                     RPDSFRLDEIQFRPAQAGPRADQTPLLFQGKAETPFHI"
     gene            complement(1463..4678)
                     /gene="recC"
                     /locus_tag="VNW28_00240"
     CDS             complement(1463..4678)
                     /gene="recC"
                     /locus_tag="VNW28_00240"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HXA08375.1"
                     /translation="MSPKAALYLHTSNHLERLADAMAQGLRRPLNDPFAAEAVVIPTL
                     GLERWLTQELALRQGICANVSFLFPQRFVAGLMDAALPGRAAARFYARENLTWGIMKL
                     LPELAARPEFADLRRYLEQPRPELRRFQLAGKIASSFDRYLAFRPRMVLDWERGTEKH
                     WQAILWRELIRSAPGLHPPALADEFSAVLRRGTAPLPERVSVFGMSTLPPFYTQFFQE
                     LAQVIEVHFFVMRPTPEWWSDIRSEREELRARRKAPATAQLDLQFARGNPLLASFGKL
                     GREFLESVTELNPTREHDQWEAPPNDTILGQIQRDIFELHDPIDSAPRSVAADDRSLQ
                     FHSCHSPMREMEVLHDQLLALFAQHSDHKPHDIVVMTPDISVYAPFIEAVFATAPQEL
                     RIPFSIADRGMRAENGVIDTFLRILESAESRYPASSVLNILESVPLQRNFGLAEPDLE
                     IIRVWIEETGIRWGIDAAHRAELGLPEFGENSWRSGLDRLLLGYAGPARGEKLFAGIL
                     AYDKVEGNLAETLGHFAAFAEALFSTARALQHPRPLSEWQDTLRQMADRFFGANDERE
                     PELHRLRRIIDSLGETAALSGFDEPVSLDVLMAHLEQALASNESGSGFLVGCVTFCAL
                     KPMRTVPFRVVCLVGMNDTSYPRHDRPPAFDLVAQDPRPGDRTTRDDDRYLFLEALLS
                     AGDVFYLSYVGRSIRDSSPIPPSVLVSELLDYTGAMVTEHPLQPFSPKYFTGTDGLFS
                     YSAENCLASGVAAEERSAPAPFIAARIGEPEAEWQRLDNAKLRSFFGNPSKFLIRERL
                     GLRLTRQDSLLEEAEPLEPGGLAKYKLEQDFLDRALSGEEFEKWFPVARAGGELPPGR
                     AGESHLRELSENARLFAEAVRANAGQRGGEPEQLQLTIGRFELSARIDNLFDSRLMRR
                     RLTTRKPADLLGAWIDHLLVNCIRSTESILITATKEKQPVIERFAPLGGESRTLLALL
                     LDFYWRGLREPLPFFPRSSLAFAERKLHPTKGSSPLEKAQKAWGDSPLPRDNEFGMGP
                     ERENDYFDLAFRNVPDPLGQEFQEIAMAIFEPALKVMTTEK"
     gene            4795..5838
                     /locus_tag="VNW28_00245"
     CDS             4795..5838
                     /locus_tag="VNW28_00245"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HXA08376.1"
                     /translation="MVRPIAVDPPRGRSRKIKQEAAEQFSRPPLARMMQLHAQLQARK
                     FPNCRKLAHELEVSAKTIQRDIEFMRYQLGLPIEYDQLHFGFVYTEPVTNFPSIQVSE
                     GEIVALFVAQKALQQYRGTPFEAPLRAAFRKIGHGLKDRITFSWSDLDAAISFRSAGQ
                     SLADLEVFEVLSHAVLESAEIEFRYRKLRSSAAETRRVRPYHLGCVDNQWYLFGFDLD
                     REQLRTFALPRIHEPRRTGARFRRPANFSIGKFLDSSFGIFQGNGRTLVRVRFQPFAA
                     QLVRERMWHHSQKIRELRDGGLEFQLQLGSLEEIERWILSWGDQAEVLEPAKLRQRLA
                     EVGRKFVAEYSGK"
     gene            5913..6590
                     /locus_tag="VNW28_00250"
     CDS             5913..6590
                     /locus_tag="VNW28_00250"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08377.1"
                     /translation="MNNNYTEIAFVLDRSGSMESCREAAMDGFNLFLHEQQQTEDLVK
                     LTLVLFDDEYLVPINGLPVAEIIPLNDENYVPRGSTALLDAIGQTVDELGARLAATPE
                     PDRPGQVIVAILTDGLENSSQSYTWQKIARAIKQQTEQYRWTFLFLGANQDAIATAAQ
                     MNIASANAATYVADSAGLRASSKSLTRKVRGIRRMHQGIASAQETQDAAAPFSQLVDE
                     EDQQERR"
     gene            6641..7024
                     /gene="folB"
                     /locus_tag="VNW28_00255"
     CDS             6641..7024
                     /gene="folB"
                     /locus_tag="VNW28_00255"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HXA08378.1"
                     /translation="MRPKNLPAAPVPNDEILIEELELTARIGVPDAERAEPQRLVVSL
                     TLQPRQRFAELDDDLGRTVDYAAVCEELQRFASGRHDKLLETLAHEMAEFLVRRFQLA
                     HVELELRKYILPETRYVAVRVTREL"
     gene            7148..7756
                     /locus_tag="VNW28_00260"
     CDS             7148..7756
                     /locus_tag="VNW28_00260"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXA08379.1"
                     /translation="MQKSNLVRDEPEAGWETVGSETHFASPHLSVAIDEVRTPTQRTP
                     RKWAVAHRKPAVVIAPLTREGKFVFIREERIPIRATIWSLPAGQIDESLEPDEAAVRA
                     VARRELREETGYELEPGAELLPLGHYFTSPGFTDEHCYFFLARPVRRATGGAQHDDTE
                     AIVDCREFSADVVRQMIADGEIRDANTLGIWARLVATGEIFP"
BASE COUNT         1590 a         2431 c         2557 g         1537 t
ORIGIN      
        1 cagatatgga acggagtttc cgctttaccc tgaaagagca gcggcgtctg atctgcgcgc
       61 ggtcccgctt gcgcggggcg aaattggatc tcgtcgagac gaaacgagtc cgggcgatcg
      121 aaaaccgtgt tcgttcccct aacgagtccg ctttccgagc gccaattcgt gcgcagggtg
      181 aactgtcgcc ggatcgcgcg cgcggcgttt aagtaggtga aaacatccgc gccacggaaa
      241 gcgtagatcg cctgcttggg atcgccgatg agggcgacgg tcgcgtcgct cccttggtag
      301 atgcgttcga agatcgaata ctgaaccgga tcggtatcct gaaattcatc cacgagcgcg
      361 gcgcggtatt tttggcggat cgattcggcc agcgccgaac cgcctgcgcc acggagcgcg
      421 tcgtcgagcc gggtgagcag atcttcaaag gcaagaacgt tctgcgcctg tttgcgacgc
      481 cgcagttcgt taggcgccca ggcgtggaac tcggcgcgca aggcggtgca aaagtccgcc
      541 gaacgatcga cggcgatttg gcaggtgccg aaaatggggt ccttcggaag cgagaacccc
      601 tttctcactc cctttgcgat gggctctgca gcgagcagct cgatgctctc gaattggcgc
      661 gcagacgcgc ctttgccgga agcaacctgt tccaaatctt ccagcacacg cgccagcagc
      721 tcggcctttt tgtagtcgcc tttggcccag ttgggattgg caaagaaagc tcggatgccg
      781 ctggcacatt ccggccagac gttgcgcacc tccttcagcg catgctccac ttgcctggcg
      841 gcttccgccg aactgcgcaa atctttcggc aaaatggtga gggtcggatt tttcaccagc
      901 tcctccaggt ctttaagaag cttcttcggc gtgatcttac cctggatcgc gaccaatacg
      961 cctaacgaat cgttggagta gaaatttcgc cgccaaaaat cccacgtcac ctcgcggagc
     1021 agatcgcgct cgtccgtgac caactcggcg tcgaagagcg tgccggtctc aaaagcctgt
     1081 tccgccagca cgcgctggca gaaaccgtga atggtgtaga tcggcgcctc atcgaaattg
     1141 gccagcgcat cctttagccg ttggatggcg gcacgatgat cgggatattt ttgcaagatt
     1201 gcaccgatca gatcgtggtc cgactttcca gcctggaagg cgccaagccc gtcgcgcaac
     1261 agatcgcgga tacggcctcg cagttcagcc gtggccagct cagtgaaggt cgtgaccagg
     1321 atcttatcga tcgttaggcc aggtcgctcc aggagcaggc gcaggacgag gccggcgatg
     1381 gtaaacgtct tcccagtgcc ggcgctggct tcaatcagaa gcaggccgtg cccgatctcg
     1441 gtttcgagga gggagaaatc gttcatttct ccgtcgtcat gaccttgagc gccggctcga
     1501 aaatcgccat cgcaatctct tgaaattcct gcccgagcgg atcgggcaca ttgcggaaag
     1561 ccagatcgaa ataatcgttt tctcgctccg gtcccatccc gaattcgtta tctcgcggga
     1621 gcggtgaatc gccccacgcc ttttgcgcct tctccagcgg ggaagatcct ttggtcggat
     1681 gaagtttccg ttccgcgaag gcaagcgagc tgcgaggaaa aaagggcagc ggctcgcgca
     1741 agccgcgcca gtagaaatcg aggagcaaag cgagcagcgt tctcgactcc ccgcccagcg
     1801 gcgcgaaacg ctcgatcacc ggctgctttt ccttggtcgc ggtgatgaga atcgattcgg
     1861 tcgagcggat gcaattcacg agtaaatgat cgatccacgc gccaagcaga tcggctggtt
     1921 ttcgtgtcgt taggcggcgg cgcatcagcc gactgtcgaa caggttatcg atccgggcgc
     1981 tgagttcgaa ccggccgatg gtgagctgca actgctccgg ctcgccgccg cgctggccgg
     2041 cgttcgccct caccgcctcg gcgaaaaggc gcgcgttctc agagagttcg cgcagatgcg
     2101 attcgcccgc ccggcccggg ggcagctccc cgccggcgcg cgccaccgga aaccacttct
     2161 cgaattcctc accactgagg gcgcgatcca ggaagtcctg ttccagtttg tacttggcga
     2221 gcccacccgg ttccagcggc tccgcttctt ccaggagcga atcctgccgc gtaagtcgca
     2281 ggccgagccg ctcgcgaatg agaaatttgg acgggttgcc gaagaaggaa cggagcttcg
     2341 cgttgtcgag gcgctgccat tccgcttctg gctcgccgat cctggcggcg atgaacggtg
     2401 ccggcgcgct tcgttcctcg gccgcaactc cgctcgcgag acagttctcc gctgaataac
     2461 tgaagagccc gtcggtgccg gtgaaatatt ttggactgaa tggctgcagc ggatgctcgg
     2521 tgaccatggc gccggtgtaa tcgagcagct cgcttacgag cacgctcggc gggatgggac
     2581 tgctgtcgcg gatcgagcga ccgacatagc tgaggtagaa aacgtcaccc gcggagagaa
     2641 gcgcttcgag gaagaggtag cgatcgtcat cgcgggtggt gcgatccccg gggcgcgggt
     2701 cttgtgccac gagatcgaag gccggcggac gatcgtggcg cgggtaggag gtatcgttca
     2761 ttccgaccag acacaccact cggaaaggaa ccgtccgcat cggcttgagc gcacagaagg
     2821 tgacgcaccc gacgagaaat cccgagccgc tctcgttgct ggcgagcgcc tgctcgagat
     2881 gcgccatgag cacgtcgagc gagaccggtt cgtcgaagcc ggaaagcgcc gcggtctcgc
     2941 ctaacgaatc aatgattcgt cgcaatcggt gcagctccgg ttcgcgctca tcattggcgc
     3001 cgaaaaagcg gtcggccatc tggcgcaggg tatcctgcca ctcgctgagc ggacgcggat
     3061 gctggagcgc gcgcgccgtt gaaaaaagcg cttccgcgaa ggcagcaaaa tggccgagag
     3121 tttcggccag attcccctcc accttgtcgt aggccaggat gcccgcgaag agtttctcgc
     3181 cccgcgcggg gccggcgtaa ccgagaagaa gacgatcgag ccccgatcgc caactgttct
     3241 cgccgaactc cggcaggccc agctcggctc gatgcgcggc gtcgattccc cagcggattc
     3301 cggtttcctc aatccagacg cgaatgatct cgaggtcggg ctcggccaga ccgaaattcc
     3361 tctgcagcgg gaccgactcg aggatgttca acacgctgct cgcggggtag cggctctcgg
     3421 cggattcgag gatgcgaagg aaggtgtcga tgacgccgtt ctccgcccgc atcccgcggt
     3481 cggcgatgct gaaaggaata cgcagctcct gcggcgcggt ggcaaagacc gcctcgatga
     3541 atggcgcgta gacgctgatg tccggcgtca tcaccacgat gtcgtgtggc ttgtgatcag
     3601 aatgttgcgc gaagagtgcg agcaactgat cgtgcaatac ctccatctcg cgcatcgggc
     3661 tgtggcagga atggaattgg agcgaacgat catccgcagc caccgagcgc ggcgcgctat
     3721 cgattggatc gtgcagttcg aaaatatcgc gctggatctg tccgagaatg gtgtcgttag
     3781 gcggcgcctc ccactggtcg tgctcccgcg ttggattcag ttcggtgacg ctttcgagaa
     3841 attcgcggcc gagtttgccg aaggaagcga ggaggggatt tccgcgcgcg aattgcagat
     3901 cgagttgcgc ggtcgcgggc gctttgcggc gggcccgcag ctcttcgcgc tcggaccgga
     3961 tatcgctcca ccactccggg gtcggccgca tcacgaagaa atgcacctcg atcacctgcg
     4021 cgagttcttg gaagaattgg gtgtaaaatg gcggcaacgt cgacatgcca aaaaccgaaa
     4081 cgcgttccgg caacggcgcg gtaccgcggc gcaagacggc gctgaattcg tccgcgagtg
     4141 ccggcggatg caaaccgggc gcggagcgga tcagctcccg ccagagaatg gcctgccagt
     4201 gcttttcggt tcctcgttcc caatcgagaa ccatgcgcgg tcgaaaggcg agataacggt
     4261 caaaagagct ggcaattttt cccgcgagtt ggaagcggcg caactccggg cgcggctgtt
     4321 cgaggtagcg gcgcagatcg gcaaactcgg gccgagcggc gagctccggg agcaacttca
     4381 tgatgcccca ggtaagattt tcgcgtgcgt aaaagcgggc ggcggctcga ccgggcaacg
     4441 ccgcgtccat caggccggcg acgaatcttt gcggaaagag gaaagagacg ttggcgcaaa
     4501 ttccctggcg cagcgcgagt tcctgggtca gccagcgttc cagaccgagc gtcgggatca
     4561 ccaccgcctc ggccgcaaac gggtcgttca gcggacgccg caacccctgc gccatcgcat
     4621 cggcgagccg ctcaagatgg ttactagtat gaaggtaaag agcggctttt ggggacactt
     4681 gcaagcacca tatcacaagc gttcggacaa aagttgtccg accaaaccga tttttctcgc
     4741 ggccaggatc gaatccactt gagctatggg tcgcgctgcc gttacaggtg agacatggta
     4801 cgtccgatag ccgttgatcc gccgcgaggt cgctcgcgca aaatcaagca ggaggccgcg
     4861 gagcagtttt cgcggccgcc gctcgcgcgc atgatgcaac tgcacgccca gctgcaggcg
     4921 agaaagtttc ccaactgccg caagctggcg cacgagctcg aggtttcggc gaaaacgatc
     4981 cagcgcgaca tcgagttcat gcgctaccaa ctcgggttgc cgatcgaata cgaccagctg
     5041 catttcggct tcgtttacac ggagccggtg acgaatttcc cgagcatcca ggtttccgag
     5101 ggcgagatcg tggcgctctt tgtcgcgcag aaggcactcc agcagtatcg cggcacgccg
     5161 ttcgaagcgc cgctgcgcgc ggcctttcgc aagatcggcc acgggttgaa ggaccggatc
     5221 accttcagct ggagcgatct cgatgccgcc atttcattcc ggagcgcggg gcaaagcctg
     5281 gcggacctcg aggttttcga agtgctcagc cacgccgtcc tggaatcggc cgagatcgag
     5341 tttcgttacc ggaaattgcg cagttccgcg gcggagaccc gccgggttcg gccgtatcac
     5401 ctcggttgcg tcgataatca gtggtatctt ttcggcttcg atcttgatcg cgagcaactg
     5461 cgcacctttg cgctcccgcg catccacgaa ccgcggcgca ccggcgcgcg ctttcggcgc
     5521 ccggcgaatt tttccattgg gaaattcctc gactcaagct tcgggatttt ccagggaaac
     5581 ggcagaacgc tcgtccgggt tcgcttccag ccttttgccg cgcagctcgt gcgcgagcgg
     5641 atgtggcacc actcgcagaa gatcagggaa ctgcgcgatg gcggactgga atttcagctc
     5701 cagctcggga gcctggagga aatcgagcgc tggatcctga gctggggcga ccaggccgaa
     5761 gtgctcgagc cggcgaagtt gcgccagcgc ctggccgagg tcgggcgcaa gtttgttgcc
     5821 gaatattccg ggaaatagaa gattcgattc ggcgcaagaa tctttgcctc cccggacagc
     5881 ggatgtctga ccccatctgt tatgattggg gcatgaacaa caactatact gaaatcgcct
     5941 tcgtcctcga ccgctccggt tccatggaga gttgccggga ggcggcgatg gatggcttca
     6001 acctcttcct gcatgagcag caacagaccg aggacctggt caagctgact ctcgtgcttt
     6061 ttgacgacga atatttggtg ccgatcaacg ggctgccggt ggcggagatc attccgctca
     6121 atgacgagaa ctacgtgccg cgcgggagca cggcgctgct ggatgcgatc gggcaaacgg
     6181 tggacgaact cggcgcgcgt ttggcggcca cgccggagcc ggatcgtccc ggccaggtga
     6241 tcgtcgccat cctgaccgat ggcctggaga attcctccca aagctacacg tggcaaaaaa
     6301 tcgcccgcgc gatcaaacag cagaccgaac agtatcggtg gacgtttctc ttcctcggcg
     6361 cgaaccagga tgcgatcgcg accgcggcgc agatgaacat cgcgtcggcc aacgccgcga
     6421 cctacgtcgc cgattctgcc ggcttgcgcg cttcctccaa gtcgctcacc cgcaaggtgc
     6481 gcggaatccg ccggatgcac cagggaatcg ccagtgcgca ggaaacgcag gatgccgctg
     6541 cgcccttctc ccagttggtg gatgaggaag accagcagga aaggcgttaa cccgcaccac
     6601 ttgattttca tcgccttggc cgtggggcgc ggggtaggtt gtgcgaccga agaacctgcc
     6661 cgcagcgcct gtgcctaacg acgaaatcct gatcgaagaa ctcgaactga ccgcccggat
     6721 cggcgtgccc gacgcagaac gcgccgagcc gcaacggctg gtcgtgagtc tcactttgca
     6781 accccgccag cgtttcgcgg aactggacga tgatctgggg cgcacggtcg attacgccgc
     6841 agtgtgcgaa gagttgcagc gttttgcctc tggccggcac gacaagttgc tcgagacgct
     6901 ggcgcacgag atggcggaat ttctcgtgcg cagattccag ctcgcgcacg tggagctgga
     6961 gctgcggaaa tacatcctgc cggaaacgcg ttacgtcgcg gtgcgagtca cgcgcgagct
     7021 gtagccgctt cgctgtgcga ggtgcgggag gtggcgccca agtgccacga ggtcatcgcc
     7081 gcgtcgccca cagggcgacg gctacagggc gattcgcttt tcgcgacgcc gtccataact
     7141 tcccggcgtg caaaagagca atctcgtgcg tgacgagcct gaggcgggtt gggaaaccgt
     7201 cggcagtgaa acgcatttcg ccagcccgca cctttcggtg gcgatcgacg aagttcgcac
     7261 accgacccag cgaactccgc gcaagtgggc ggtggcgcac cgcaagcccg ctgtggtcat
     7321 cgcaccgctg acacgcgagg gaaaattcgt cttcatccgg gaagaacgca ttccgattcg
     7381 ggcgacgatc tggtccctgc ctgcgggcca gatcgacgaa tcgctcgagc cggatgaagc
     7441 ggccgtgcgc gccgtggcgc gacgggagct gcgcgaagaa accggttatg agctggagcc
     7501 gggcgcggaa ttgctcccgt tagggcacta ttttacctcg cccggtttta ccgacgagca
     7561 ctgctacttt ttcctcgcgc ggccggtgcg gcgggcaacc ggcggcgcgc agcacgacga
     7621 cacggaagcg atcgtcgatt gccgcgagtt cagcgccgac gtcgtgcgcc agatgatcgc
     7681 cgacggcgaa atccgtgacg ccaacaccct cggtatctgg gcccggctgg tggcgaccgg
     7741 cgagattttt ccctaagcgg agaaaattcg aatgtcgaat atcgaactcc gaaacgaaca
     7801 gggaaaatcg aaagaaaggc tcgaaagctt gaaacttcga gcgctgtcgt tttccgggtt
     7861 ttccgcgttt tttgagcctt cgaaattgtt tcgatcttcg atccgcctca ggcggatcgg
     7921 atttcgaatt ttcccaacgg aatgaagctg cgcgacatct ttgcctttcg cgatccgccc
     7981 gagagcgaga cctcgaagcc gtttctcgag cacctcgagg atctgcgctg gatgatcgtg
     8041 aaaatggcgg ccgtgctgac tgtggcgatg tttttctgtt tcgcctttcg gcaacggctg
     8101 gtcgtgattc tgcaa
//
LOCUS       DATMNS010000009         5090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4668052, whole genome shotgun sequence.
ACCESSION   DATMNS010000009 DATMNS010000000
VERSION     DATMNS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4668052"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..925)
                     /locus_tag="VNW28_00265"
     CDS             complement(110..925)
                     /locus_tag="VNW28_00265"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA08380.1"
                     /translation="MSEAMLERSATTATLAGARAKPAWSDALRHILTIAKREVSGYFA
                     SPVAYVFIVIFLLLSGFLTFMVAGFFEHGQANLLSFFAWHPWLYLFLVPAVGMRMWSE
                     ERRLGTMELLLTMPITPWQAIVGKFLASWLILALALALTFPLVITVNYLGHPDNGVIF
                     TGYLGSLLLAGAYLSIASVTSAMTRNQVVSFILSLVVCLFLILAGWPPVTDLLTQWAS
                     PWFVQTIVAFSVVTHFDGLQVGVIDSRDVLFFLSVILFSLFTTSVILRAHRAG"
     gene            complement(922..1875)
                     /locus_tag="VNW28_00270"
     CDS             complement(922..1875)
                     /locus_tag="VNW28_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA08381.1"
                     /translation="MVTVRNLKKSFGAKRAVDGVSFTVQAGEVLGFLGPNGAGKSTTM
                     RMLTGFIPPSEGEITVGGFNMLEDPIPAKRLIGYLPENAPSYTDMTVLGFLRFAAELR
                     GLRGEARQHAVQRVVEMCFLEPVIHQSIDTLSKGYRHRTCFAQSIIHDPQVLVLDEPT
                     DGLDPNQKHEVRSLIRKMGESKAIIFSTHILEEVEAACSRAIIIDRGQIVANGTPEEL
                     KQRSTYAGAVTLRIADGSVAQVRQKLEQIPAVAKCVVLSTTPVALRAFAKNNGGDLAR
                     AIGELAAAERWRIEELHTEEGRLDEVFRNITLPETKREEKA"
     gene            2028..2990
                     /locus_tag="VNW28_00275"
     CDS             2028..2990
                     /locus_tag="VNW28_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HXA08382.1"
                     /translation="MKAIRVEQFGEPEVMKLIEVPPLQPGRGEVLVRMHAVGVNPVET
                     YIRAGTYARKPALPFTPGNDGAGVVELVGEGVSEFRAGDRVYLAGASSGTYAEYALGN
                     AAQVHPLPEPASFAQGAAMGTPYATAYRGLFQRAEAQPNETVLVHGASGGVGTAAVQL
                     ARARGLRVLGTAGTERGRELVREQGAREVFDHHAPDYLAQILKATSGRGVDVILEMLA
                     NLNLGKDLTILAPRGRVVVIGNRGRVEIDARETMSRDADVRGMVLPNTPPAELASIHA
                     ALVAGLANGSLRPVIGKEFPLRDAPGAHRAVLEPGAFGKIVLVP"
     gene            complement(3018..3647)
                     /locus_tag="VNW28_00280"
     CDS             complement(3018..3647)
                     /locus_tag="VNW28_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08383.1"
                     /translation="MLDSPELRKLRSLKTPVGIQRFLDAMPYHLAPTAWSPRVVLRQR
                     TAHCLEGAIFAAAALRVLGFPPLLLDLEADNDTDHVLAVFKVRGHWGAVAKSNFTGCR
                     YREPVYRTLRELALSYFHIYFNLRGERTLRRYSRPVNLARFDRDQWMTSEKNIWVVPN
                     HLCDIPHTPLLKPAQVKNLTRLDGRTVQGEIVGHRRNSTTPRPRIFRQR"
     gene            complement(3736..4593)
                     /locus_tag="VNW28_00285"
     CDS             complement(3736..4593)
                     /locus_tag="VNW28_00285"
                     /inference="COORDINATES: protein motif:HMM:NF019947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXA08384.1"
                     /translation="MPILSTGFGSDVTVNVIASIIASAILLGAGYLWGKYKERRRYGR
                     KLEEYDFYPYIINRDNFPEFSLKDFRLGMHYFLRNFDPMAARQLIFIGEQNNVRAQLE
                     PTEQKVYARLFTRYDGKKIADDTQEYLGNYARIVRLLGKSFPNLGIEILLHDLSNPSR
                     SLVALENNVTGRNLHDGTTNLVIDLKKRQMTHEEKLNYELNIGARRFKCTTIPITRPE
                     YGVVGAVCINVDANYLCEEALSAADKVATFLQTFCRTEMQLDENILSRDEYAKALKGK
                     RHFKDNRFE"
     gene            complement(4606..4782)
                     /locus_tag="VNW28_00290"
     CDS             complement(4606..4782)
                     /locus_tag="VNW28_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08385.1"
                     /translation="MRKTALIGFVVGMALGCLCGALTSLLPTGVEGNANEILSVNEGT
                     GNAQLHLKGKPEIK"
BASE COUNT         1039 a         1415 c         1659 g          977 t
ORIGIN      
        1 cctgcgcggc gatgagattg atcgcgatca cgaggaaaag catcgcgacg atgcccacgc
       61 cggaataaag tatgttttcg attcctttct ttttcatgcg ctttactcgt tagccggcgc
      121 gatgcgcgcg gagaatgacg ctggtggtaa aaagcgagaa gagaatcacg gagaggaaaa
      181 agagcacgtc gcgcgaatcg atcacgccca cctgcaggcc gtcgaagtgg gtgacgacgc
      241 tgaaggccac gatcgtctgc acgaaccagg gactggccca ctgcgtcagc agatccgtca
      301 ccggcggcca gccggcgagg atgagaaaga ggcaaactac gagcgagaga atgaagctca
      361 ccacttgatt gcgcgtcatc gccgaagtca cggaagcgat ggaaaggtaa gcgccggcca
      421 gcaacaggct gccgaggtaa ccggtgaaga tcacgccatt atccgggtgt ccgagataat
      481 tgacggtaat gacgagcgga aaggtgaggg cgagcgcgag cgccaggatg agccaggaag
      541 cgagaaattt gccgacaatc gcctgccagg gcgtgatcgg catcgtcaac aaaagttcca
      601 tcgtgccgag gcggcgttct tccgaccaca tccgcatccc gactgccggc acgaggaaaa
      661 gataaagcca cggatgccag gcgaagaaag aaaggagatt ggcctgcccg tgctcgaaga
      721 agcctgcgac catgaaagtg agaaatccgg agagcagcaa aaaaatgacg atgaaaacgt
      781 aggccaccgg tgaggcgaaa tagccgctca cttcccgttt ggcgatggtg agaatgtggc
      841 ggagcgcatc gctccacgcg ggctttgcgc gcgcgcccgc gagcgtggcg gtggtggccg
      901 atcgttcgag catggcttcg ctcatgcctt ctcctcccgc ttcgtttctg gcagcgtgat
      961 attgcggaag acttcgtcga ggcggccttc ttcggtgtgc agctcctcga ttctccagcg
     1021 ctcggccgca gccagttcgc cgatggcgcg cgccagatct ccgccgttat tcttcgcgaa
     1081 agcgcgcagc gcgaccggcg ttgtcgaaag caccacgcac ttcgcgaccg ccgggatctg
     1141 ctccagcttt tgccggacct gcgcgacgct gccatcggcg atgcgcaagg tgacggcgcc
     1201 ggcgtaggtc gagcgctgct tcaattcctc cggcgtgccg ttggccacga tctggccgcg
     1261 gtcgatgatg atcgcgcgcg agcaggccgc ctcgacctcc tccagaatgt gagtcgaaaa
     1321 aatgatcgcc ttcgattcgc ccatcttgcg aatcaagctg cggacttcgt gtttttgatt
     1381 gggatcgagt ccgtcggtcg gctcatcgag cacgagcact tgcggatcat gaatgatcga
     1441 ttgcgcgaaa caggtgcgat gccgatagcc cttcgacaac gtgtcgatgc tctggtggat
     1501 gaccggttcg agaaaacaca tctcgaccac ccgctgcacg gcgtgctggc gcgcttcgcc
     1561 acgcaggccg cgcagctccg cggcgaagcg gaggaagccg agcaccgtca tgtccgtgta
     1621 ggagggggcg ttctccggca ggtagccgat caggcgtttc gccgggatcg ggtcttccag
     1681 catgttaaaa ccgccgaccg ttatctcgcc ctccgacggc gggatgaatc ccgtcagcat
     1741 gcgcatggtg gtcgatttgc cggcgccatt cggcccgaga aagccgagca cctcgcccgc
     1801 ctgcaccgtg aacgagactc cgtccaccgc ccgcttggct ccaaacgatt tcttgagatt
     1861 tcgaactgtt accatgccgc cgaggttcgt tttttcggat gtgcatgtgt aggacacaca
     1921 atttttcaaa acgttcaagt gggaactttg attgcccgtt ttcctcggaa caaaagggcg
     1981 cagtttcgat ttggagaaaa cgaatcggca aagcagtatg cagcgcgatg aaagcgatcc
     2041 gagtggaaca gtttggggag cccgaggtga tgaaactgat cgaggtcccg ccgttgcagc
     2101 cgggccgggg tgaagtgctc gtgcgaatgc acgcggtcgg cgtgaacccg gtcgagacct
     2161 acatccgtgc cggcacctac gcgcgcaagc cggccctccc ctttacgcct ggcaacgacg
     2221 gtgcgggcgt ggtcgaactg gtgggcgaag gggtcagcga gttccgggcc ggtgatcggg
     2281 tttacctcgc gggcgcaagc agcgggacct acgcggagta tgccttgggc aacgcggcgc
     2341 aggtacatcc gttgccggaa ccggcttcgt tcgcgcaagg cgcggcgatg gggacgccgt
     2401 acgcaacggc gtatcgcggg ttgttccagc gcgcggaggc gcagcctaac gaaaccgtgc
     2461 tggttcacgg cgcgagtggc ggggtcggca cggccgcggt gcagctggcg cgggcgcgcg
     2521 gcctgcgcgt gctcggaacg gccggcaccg agcgcggacg cgaactggtg cgcgagcagg
     2581 gcgcgcgcga ggtcttcgat catcacgcgc cggactacct ggcgcagatt ttgaaggcaa
     2641 cttccgggcg gggcgtggat gtgattttgg aaatgctcgc gaacctgaat ctgggcaagg
     2701 atctcaccat cctcgcgccg cgcggccgcg tggtggtgat tggcaaccgg ggtcgggtgg
     2761 aaattgatgc gcgcgagacg atgtcgcgcg acgccgatgt tcgcggaatg gtgctgccga
     2821 atacgccgcc ggccgagctg gccagcatcc acgccgcgct cgtggccggg ctggcgaacg
     2881 ggagcctgcg tccggtgatc ggaaaggagt tcccactgag ggacgcgccg ggagcgcatc
     2941 gcgcggtgct ggagccggga gcgtttggga agatcgtgct cgtcccgtga cggggtgggg
     3001 aacagagggc cgattcgcta gcgctggcgg aagattcgcg ggcgcggcgt ggtgctgttt
     3061 cggcggtgac caacgatttc gccttgaaca gtccggccgt ccaggcgcgt gagatttttc
     3121 acctgggctg gcttcaagag tggcgtgtgc gggatatcgc aaagatggtt tggcacgacc
     3181 cagatgtttt tctctgaggt catccactgg tcgcgatcga accgagccag gttaaccggg
     3241 cgagagtagc gacgcagggt gcgctcgccg cgcaggttaa aatagatgtg gaagtaactc
     3301 agggccagct cgcgcaatgt gcgatagacc ggctcgcggt atctgcaccc ggtaaagttc
     3361 gattttgcca ccgcacccca atgaccgcgg actttgaaga cagcgagaac atgatcggta
     3421 tcgttgtccg cttccaggtc gaggagcaat ggcgggaagc caagcacacg cagagcggcc
     3481 gcggcgaaga tcgcgccctc cagacaatgc gcggtccgct ggcgcaggac gacgcgcggc
     3541 gaccaggcgg tcggcgcaag atgatagggc atcgcgtcga ggaaccgctg gatgccgaca
     3601 ggcgtcttga gggagcggag ttttcttaat tccggcgaat caagcatgga ggaaagcgac
     3661 aaatgacaag tgacgagtga gaccgaccca tagctgacga ctaataactg ttaagcaaaa
     3721 accaacggct ttctcttact cgaaccggtt gtctttgaaa tggcgtttgc cttttagcgc
     3781 tttggcgtat tcgtcgcggc tgagaatgtt ttcgtcgagc tgcatctcgg tgcgacagaa
     3841 ggtctgcaaa aaggtcgcca ctttgtccgc ggcgctcagg gcttcctcgc agaggtagtt
     3901 agcatccaca ttgatgcaca cggccccgac cacgccatac tctggccggg taatcgggat
     3961 ggtggtgcac ttgaagcgcc gcgcgccaat gttcagctca taattcagct tctcttcatg
     4021 ggtcatctgc cgcttcttca ggtcgatgac caggttggtg gtgccatcat gcaggttgcg
     4081 gccggtgacg ttgttctcca gcgcgacgag cgagcgcgat ggattggaaa gatcgtgcag
     4141 caggatctca atcccgaggt tggggaatga tttgcccagc aggcgaacga tccgggcgta
     4201 attccccaga tattcctggg tgtcatccgc gatttttttg ccgtcgtaac gcgtgaagag
     4261 gcgggcgtaa accttctgct cggtgggttc cagctgcgcc cggacattgt tctgttcgcc
     4321 aatgaaaatg agctgtcgcg ccgccatcgg atcgaaattc cggaggaagt agtgcattcc
     4381 aagccgaaag tccttgagac taaattccgg gaagttgtcg cgattgatga tgtagggata
     4441 gaagtcgtat tcctcgagct tgcgcccgta gcgccgccgc tccttgtatt ttccccaaag
     4501 atagccagcc ccgagaagaa tggcggaagc aatgatggaa gcgatcacgt tgaccgtgac
     4561 gtcgctccca aacccagtgg ataagattgg catggtgtct ccggatcact tgatctcggg
     4621 cttgcctttc aggtgaagct gggcattgcc cgtgccttcg ttcacgctta atatttcatt
     4681 cgcgttgccc tcaactccgg ttgggaggag gctggtcaat gctccgcaaa ggcagcccag
     4741 cgccatgcca acgacaaatc cgatcagggc cgtttttcgc acctggcaat ctgcgacctt
     4801 caagatcgag ttcaagcagt aaggggagat agaggagtgg gaccggagct ccggcttgtc
     4861 agttgatcga ttcagattgc gagccagcgt cggaggcggg atccctcgct gccactcgct
     4921 cgaatgagcg gtgcctgata ttgtgctggg cgaaaagctg gcgccgggat tccgaaacga
     4981 gcgggagacg cgaggtagcg gggcaccgga tccgtggtct atgccttacg cggtcgggca
     5041 tggaagctgc ttttaaacat ggtgaatata gagaaaccac agccaaccac
//
LOCUS       DATMNS010000010         2810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_374537, whole genome shotgun sequence.
ACCESSION   DATMNS010000010 DATMNS010000000
VERSION     DATMNS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374537"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VNW28_00295"
     CDS             <1..278
                     /locus_tag="VNW28_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08386.1"
                     /translation="LRGMEALAVLISQDYSEEDSRLLRQPVLFDYFEKQLAEMNVGLR
                     TNPTVVRMNAGIQKVRRILGCGDAPISQKKAAEVSGVLGELVKSLSR"
     gene            complement(268..1278)
                     /locus_tag="VNW28_00300"
     CDS             complement(268..1278)
                     /locus_tag="VNW28_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXA08387.1"
                     /translation="MRVAIGMRVMVLESPRQPLVARDVPRPAPRPDQLLVRVAACAVC
                     RTDLHVLDGELPEPKLPLVLGHEIVGRVEEIGAEVRGFHPGQRVGIPWLGWTCGECKF
                     CLTNRENLCLRARFTGYTIEGGYAEYTVADARFCFPMPDAYEDVAGAPLLCAGLIGYR
                     CLRKAGDARRLGIYGFGAAAHIIAQVARHEEREVFAFTSPGDTTAQNFARRLGAKWAG
                     GSDETPPETLDAAIIFAPVGPLVPAALRVLERGGTVVCGGIHMSDIPSFPYNDLWQER
                     TICSVANLTRRDGTEFLALAPQVPVRVETKTFPLAQANEALHALRRGALTGAAVLVPP
                     ND"
     gene            1374..>2810
                     /locus_tag="VNW28_00305"
     CDS             1374..>2810
                     /locus_tag="VNW28_00305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXA08388.1"
                     /translation="MVDVFRYSLGKERARVTKTRSTVMEHSLFSETISTAVARLATDA
                     KTGLSTSEARARLSRDGPNQLAETTPPPWWHKLLEQFNQLVIWILLAATILSGALGDW
                     LEAGAILAIVVLNALLGFFQERKAEEALSSLRKLSSPSACVVRDGSLHDLAAAELVCG
                     DIVDLGAGDIVPADLRLLRNFGLKMQEAALTGESTPVDKDAEAVLPNDSTIAERINMV
                     FLGTNVVAGRARGLVVATGMRSELGRIAGFLQSEQREPTPLQKRLEELGRVLILACLA
                     LVALIFVLQLWRGESLAHTFLLAISLAVAAVPEGLPAVVTIALALGLQRMVKRHALIR
                     RLASVETLGSVTVICSDKTGTLTRNEMTATEIVTASEHYRITGSGYTPEGEFLGNSGP
                     AAQPLPAELARALQIGLWCNISQLEAPDEKDGRWRIVGDPTEGALVVAARKAGLQRDP
                     ADVILHEIPFDSERKLMSMVVQDAHEQTF"
BASE COUNT          517 a          907 c          905 g          481 t
ORIGIN      
        1 ggttgcgcgg aatggaagcg ctcgccgttc tcatctcgca agactattcc gaagaggatt
       61 cccggctcct gcggcaaccg gtgctctttg attatttcga gaaacaactc gcggagatga
      121 acgttgggct gaggacgaac cccaccgtcg tccgaatgaa cgcaggcatc cagaaagtgc
      181 gccgcatcct tgggtgcggc gacgcgccga tctcgcagaa aaaagccgcg gaagtctccg
      241 gcgttttggg cgagctggtc aaaagtctca gtcgttaggc ggaacgagca cggccgcgcc
      301 ggtcagcgcg ccgcggcgca gggcgtggag cgcttcgttc gcctgcgcga gcgggaatgt
      361 tttggtctcg acgcgcaccg ggacctgcgg cgcgagggcg agaaattccg tgccgtcgcg
      421 ccgggtgaga ttcgcgaccg aacagatcgt gcgttcctgc caaagatcgt tatacgggaa
      481 tgacgggatg tcgctcatgt gaatcccgcc gcagacgacc gtgccgccgc gttcgaggac
      541 gcgcagcgcg gccggcacga gcggaccgac tggcgcgaaa atgatcgccg catccagcgt
      601 ctcgggcggc gtttcatccg agccgccggc ccatttcgca ccaagccggc gcgcgaaatt
      661 ctgcgcggtt gtatcgccgg ggctcgtgaa cgcgaacact tcgcgttcct cgtgccgggc
      721 cacctgcgcg atgatgtgcg ccgcggcgcc gaatccgtaa atcccgagcc gccgagcgtc
      781 gccggctttg cggagacagc gatagccgat caatcccgcg cagaggagcg gcgcgcctgc
      841 gacatcttcg taggcgtccg gcatgggaaa acagaaacgc gcatccgcga cggtgtattc
      901 cgcgtagccg ccctcgatcg tgtaaccggt gaaacgcgcg cgcaggcaga gattttcacg
      961 attcgtcagg caaaatttgc attcgccgca ggtccagccg agccacggaa tcccgactcg
     1021 ttggccaggg tggaagccgc gcacctccgc gccgatctcc tcgacccggc cgacgatttc
     1081 gtgaccgaga acgagcggca gtttcggctc gggcagttca ccgtcgagca catggagatc
     1141 ggtgcggcag accgcgcagg ccgcgacgcg cacgagtaat tgatcggggc ggggcgcggg
     1201 gcgcggcacg tcccgggcca cgagcggctg gcggggcgat tcgagaacca tcacgcgcat
     1261 accgatggct acacgcattc gatcgctttt gaaaacgcaa cgcaatttcc ggccactgaa
     1321 aggcaggcaa cggttcgcgc atccgccgaa gcgctgtctc cgatcatcgc caattggtcg
     1381 acgtttttcg ttattcgtta ggcaaggaac gggccagagt aacgaagaca aggtcaaccg
     1441 tgatggagca ctccttattt tccgagacga tctccacggc cgtcgcacgt ctcgcgacgg
     1501 atgcgaagac gggtctcagc acctccgagg cacgggcgcg actctcccgc gacggtccga
     1561 accagctcgc cgaaacgacg cccccgccct ggtggcacaa gctgctcgag cagttcaatc
     1621 agctcgtcat ttggattctg ctcgcggcca ccatcctctc cggcgcgctc ggcgactggc
     1681 tggaggcggg cgcgatcctc gccattgtcg tgctcaacgc gctcctcgga ttttttcagg
     1741 aacgcaaagc ggaggaagcg ctctcctcgc tgcggaaact ttcctcgccg agcgcgtgcg
     1801 tggtgcgcga cggatccctg cacgatctcg ccgcggccga actggtgtgc ggcgatatcg
     1861 tcgaccttgg agctggcgat atcgtcccgg cggatctccg tttgctccgc aactttggcc
     1921 tgaaaatgca ggaagcggcg ctcaccgggg aatcgacccc ggtggacaag gacgccgagg
     1981 ccgtcttgcc taacgactcg acgatcgccg agcggatcaa catggtcttt ctcgggacca
     2041 acgtggtggc gggccgggcg cgcggcctgg tcgtcgccac cggaatgcgg agcgagctgg
     2101 gccgcatcgc gggctttctg caaagcgagc agcgcgagcc gacccccctg caaaagcgtt
     2161 tggaggaatt gggacgcgtc ctgatcctcg cctgcctcgc gctcgtcgcg ctcattttcg
     2221 ttctccaact atggcgcggg gagagtctcg cccacacctt tctcctcgcc atcagcctcg
     2281 cggtcgcggc ggtgccggaa ggtttgcccg ccgtcgtgac gatcgcgctc gccctcggct
     2341 tgcagcggat ggtgaaacgc cacgcgctca tccgtcgcct cgccagcgtc gaaaccctcg
     2401 gctcggttac ggtcatttgt tccgacaaaa cgggcacgct gacgcgtaac gaaatgacgg
     2461 cgacagaaat cgtcaccgcg agcgagcatt accgaatcac gggcagcggt tacacgccag
     2521 aaggcgagtt cctgggaaat tccggacccg ccgcgcagcc gctgccagcg gagctcgcgc
     2581 gcgccctcca gatcgggctt tggtgcaaca tctcgcagct cgaggcgccg gacgagaagg
     2641 acggacgctg gcggattgtc ggcgatccga ccgaaggcgc gctcgtcgtc gccgcacgca
     2701 aggccggcct gcagcgggat ccggctgatg tgatcctcca cgaaattccc ttcgactcgg
     2761 aacgaaaact gatgtcgatg gtggtccagg acgcgcacga acaaaccttt
//
LOCUS       DATMNS010000011        11792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3587398, whole genome shotgun sequence.
ACCESSION   DATMNS010000011 DATMNS010000000
VERSION     DATMNS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 11792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11792
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3587398"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..433)
                     /locus_tag="VNW28_00310"
     CDS             complement(182..433)
                     /locus_tag="VNW28_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08389.1"
                     /translation="MTEPATSIPQEEIAQLQKKFSEIKHSINNALAVMMALSEMSQRR
                     PDYAEKLASTVLAKAPQIVTSLQEFTQVLNEKAGPKPAA"
     gene            503..3421
                     /gene="uvrA"
                     /locus_tag="VNW28_00315"
     CDS             503..3421
                     /gene="uvrA"
                     /locus_tag="VNW28_00315"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXA08390.1"
                     /translation="MPAKSIKISGARQHNLKNLHVEIPREKLVVITGMSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLDQMEKPDVDFIEGLSPAIAIEQRSAGTNPRSIIAT
                     TTEIYDYLRVLFSAVGQPHDPLTGQPIFRQTAQQIVDQILAYPAETKIILLAPLVRGE
                     SGEFRDVIEKTRREGFVRIRVDGEIVELGRPEPIRLKKGERHTIEAVVDRLVMREGIR
                     ARLADSVETALKWGGNKMVVLREGKTSNVQCPTSNVQLEEDWEEVRYSTDYGNPETDF
                     SLGQLTPRHFSFNSHLGACPACHGLGTQLVCDPELMVTDTSKSLAEGAVVPWRRGTKR
                     MQAYYRGLQTALVRHFDVADNIPFEDLPEKFKEALYFGTGDRPIEMSFGGNGRGTKTA
                     RPFEGLLPQLQRLYGETESEFTRSRIRAFMNRAPCTTCHGARLKPEILAVTINAQSGR
                     EANIHQFCELTIAAAAQFVRQLDLSEPQRALVSEVVHEISSRLEFLLEVGLDYLTLNR
                     ESGTLSGGEAQRIRLATQIGSGLAGVLYVLDEPSIGLHQRDNARLIGTLRRLRDLGNS
                     VIVVEHDEETIRAADHILDLGPGAGPRGGEIVAAGTLEEILQAQNSPTAAYLSGRSRI
                     AIPRQRVAPRSNDPSSDGWLTIVGATENNLRNLTAAIPLGCLTCVTGVSGSGKSTLID
                     DILRRALFRHFYNSKDKPGEHRALHGLDQIDKAIIIDQSAIGRTPRSNPVTYTGAFTP
                     IRELFSQLPAARVRGYGAGRFSFNVKGGRCENCQGDGLIKIEMHFLADAYAVCEVCQG
                     KRYNRETLEITYKGKNVADVLALTVDEASRFFRNVPTVADKLRALQDVGLGYLRLGQA
                     GTTLSGGEAQRVKLATELAKRATGRTLYILDEPTTGLHFADIEKLLQVLMKLRNAGNT
                     LVVIEHNLEMIKCADWVIDLGPEGGEGGGRIVGVGTPEAIAGMKGSHTGAYLRPLLGK
                     "
     gene            3418..5877
                     /locus_tag="VNW28_00320"
     CDS             3418..5877
                     /locus_tag="VNW28_00320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA08391.1"
                     /translation="MNRKFPALLSAVLLSLGSASRGQQTPALEAAVAPLSDGVPEVAV
                     ERLKTLLAQNPPAAEQLVAQRKLAEALVEMGRPADALEVVASPGLAKDPEAVFWHAQA
                     LAALGRWAEALPFYQAIARNEKSSRRTEALFGEAEMLRALGQTDEALPVLLKLENAPE
                     WSVRAKLGVAQLMIERGDLAAADRLLRETRPQRATERTEKRFLFGRLSLAQGRAEKAI
                     ETLTVILKKPADVSHPLLIATLFALADAHLKAKTPEIGDDALEEFIDHHPNDPALPTI
                     FAKLDELYRMERKPSTRELERWMRDPAEPRRALAQWYLARSKLRSGNRESAIELLTQV
                     RDSAVRLPSLGEADLDLARLHLAKDQWEPAIAAAEAARAQNSAPAFLGQVEWLTAEAN
                     YRSGHLAQAAPIYEKLALQAPTVSGDALFNAALCWLRLDRAEEFSADYRKISSDPAKQ
                     AVQGELLLEQGAAQAAQGKGEAGLTFQQFIRDFPQSQRVSEAWVALAELAFHAAKPDI
                     VTARKDLAQARQGHPTQAALERADYLQIWLEDATESPDETPVITAANNFLQQYPDSRF
                     IAEVRMKIAEAYFRRQDFANAQTQFELLAQQKPDGPLAEKALFFAARSAMSSMGAESL
                     DHALALLDQVVKLNGDLKWVARNEEAAIERRLGKNREALALYDEVLKNDAKAAENREA
                     LCGKGDILYEMGVTDPQNYRRAIELYDQLATESGVSAHWRNQAEFKKGKALEKLNDQP
                     GALATYYGVIEKGARPDVQHEFFWFYKAGFNAAHLLEEAKDWKAAVAVYRKLAAVGGT
                     RSDEAKARLTQLRLEHFLWDE"
     gene            5948..6259
                     /locus_tag="VNW28_00325"
     CDS             5948..6259
                     /locus_tag="VNW28_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08392.1"
                     /translation="MKTRSGWFHLRGLFLLAVGFTMGIVLSATALGGPNTDRAAMIAR
                     SRVARPHMAQDRPAPKKVVFYVMSSASPIPKPIAYFSAGVPTTTIPMEIIGRGPESGP
                     R"
     gene            complement(6284..7285)
                     /gene="ilvC"
                     /locus_tag="VNW28_00330"
     CDS             complement(6284..7285)
                     /gene="ilvC"
                     /locus_tag="VNW28_00330"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXA08393.1"
                     /translation="MLARILTDKDVNLAPLLDKTCAVVGYGAQGRAHALNLRDSGIPV
                     IVGLLPKSRSRVTARQDGLRLTTTAAAVRQADVVFLALPDTKMAGIYEVDIAPHLRPG
                     QTLLFAHGFAVHYRTIVPPNEVNVVMVAPKGLGAMVRAQFVEGKGVPCVVAIQQDANG
                     HARQLALAYAKAIGGTRAGVLETTFQEETEADLFAEQAVLCGGMSALVQAGFETLVEA
                     GYQPEMAYFECLHELKFIVDLMHRAGISGMRERISETAKWGDISVGPKIIDESVKLRM
                     KEALLKIRRGKFAQEWLRETATGRKRYQRLLDKGQAEPIEHVGARLRRLMPWLDEKS"
     gene            complement(7360..9360)
                     /locus_tag="VNW28_00335"
     CDS             complement(7360..9360)
                     /locus_tag="VNW28_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA08394.1"
                     /translation="MVRTPSLSGAFLWDDEYLARDSPFIKSPLLVLEVFRHYLFPDSF
                     SGHYRPIQNLSFMADYFFWNTNTFGFHLTNLLLHAGSAVMLYFLVKRLLQAARDAAWL
                     RDGSNFLADLAFFVALLWAVHPVHSAAVDYISGRADSLAFLFASSAWYLVFRARAMKP
                     GTGRYLLFLAATLLGLLALCSRETAGLWLVFFLFHTLFFDHKIRLRGKTWSVAVCVAL
                     FALYMGLRQLPPPRSGPGPSEGWGAPVRAVLMLRALGDYGRLMIFPSNLHMERTVVDG
                     DNYRSHTTWEHSVATEYLSIAGLIFAFLLIAGCCWRGNGRSLRIFGASWFIISYLPTS
                     NIVELNATCAEHWLYLPSVGLLLFLAGCVLDLPARYRASLAAFACVAAGGLSIRSALR
                     SSDWVTPEVFYERTMAAGGNSMRVSGNLAELYTRSGKLAKAEALLRKVLAGTPDFPVA
                     RNNLAHVYYEEGKTKEAEALFADSAKHSLEASKTYPRAWIAVLNLGRLLHATHRDDEA
                     LAALEKARTDYPEVWEIVSLEAELLRQKKKSANAFALVSDFARSHWWHYGAALALGRL
                     YAQAGDADRAVEAFRTASWLDLHEVDALNLIALMRLRQNRLADAYRAQKRAVARQPDA
                     PSQYALLSDILEKMGRKAAAKDAIAQVTRLEAIGRPSPALAN"
     gene            complement(9598..9999)
                     /locus_tag="VNW28_00340"
     CDS             complement(9598..9999)
                     /locus_tag="VNW28_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXA08395.1"
                     /translation="MVSRLGKDRWGFTLTELMVVVAIIALLSALAIPGFLRARKRVQA
                     GRILNDLRLIVSAVDQYAIENSKATGAVVPVTAWTSYMKQNSLLYNTGQDMFGQDYGP
                     QTVDSLPKIPLFTWNALSDVAGTAYFSPYTP"
     gene            complement(10259..10660)
                     /locus_tag="VNW28_00345"
     CDS             complement(10259..10660)
                     /locus_tag="VNW28_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXA08396.1"
                     /translation="MLQKLNKNRGGFTLVEIMIVVAIIALLAAIAVPGFLRARKRAQA
                     SRILNDLRLIDSAVDQYAIENSKVTGTIVPTAAWTQYMKANSILYNTGQDMFGDAYGS
                     QSVDSLPKVPANAWATLSDVAGTAFFSPYGP"
     gene            10787..>11792
                     /locus_tag="VNW28_00350"
     CDS             10787..>11792
                     /locus_tag="VNW28_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08397.1"
                     /translation="MSHGGRLMNLLSKSSSLRAVPTGVYLFLGVFLLRLIVLVRLTGS
                     QFLLPTAGDMQFYNEWALRILRGSWTEHTAFYGLPLYPFLLAGIYKICGYSPFVPGLL
                     QAGCEGGTAVLLYQLSALVFPARGVADSAEEKKPSINVIGLGAVIGWAFFQPAQVYSV
                     ILMPTAWLVFVFWFVVWQIVKRREAPALWKLLLMGMLIGFTAMGIATILFLVPLVFAA
                     LFLRWAAPFSYRVAGTVAVIAGIFVGASPAWIHNAFVARDPVFLSAHSGVNFWIGNNP
                     MANGYPRFPPGLHAGQQAMLQDSIATAEKAAGHPLKRSKVSAFWSQKARAWIQGHPLD
                     W"
BASE COUNT         2325 a         3444 c         3779 g         2244 t
ORIGIN      
        1 cagccggccg cgggcctcgc ttgagattgc cgagctgttg ttggcgttgt agggccgggc
       61 gtgtcgcccg cttgagggac ggcgcagtcg gtctttctcc tgcggtcgtc gttctccgcc
      121 tgcggccgat actccgaagg cgttcgcgag aaggcaggcc gccgctacac tttcgaactg
      181 ctcaggccgc cggctttggc ccggcttttt cgttcaggac ctgcgtaaac tcctgcaggc
      241 tcgtcacgat ctgcggcgcc ttggccagga cggtgctggc cagtttctcg gcgtaatcgg
      301 gccggcgctg cgacatctcc gagagcgcca tcatgaccgc gagcgcgttg ttaatcgagt
      361 gcttgatttc gctgaatttc ttctgcaact gcgcgatttc ttcctgtggg atgctggtgg
      421 cgggttcggt catgatcggg tcaaattagc tggagaaagc gggcgggtgt gcttgactga
      481 taggttcgac agattccgtc cgatgcctgc aaagagtatc aagatcagtg gggcgcgcca
      541 gcacaatcta aagaatctcc acgtcgagat tccgcgggaa aagctggtcg tgatcactgg
      601 gatgagtgga tccggcaaat cgtcgcttgc cttcgatacc ctctacgccg aaggccagcg
      661 ccgctacgtc gagagcctct ctgcctacgc ccggcaattc ctcgaccaga tggagaaacc
      721 ggacgtcgat ttcatcgaag gcctctcgcc ggcgatcgcc atcgaacagc gcagcgcggg
      781 gacgaatccg cgctcgatca ttgcgacgac gaccgagatt tacgattacc tgcgggtctt
      841 gttttcggcg gtcgggcagc cccacgatcc gttgaccggc cagccgatct tccggcaaac
      901 ggcgcaacaa attgtggatc agattctcgc gtatccggcg gagaccaaga tcatcctgct
      961 cgcgccactg gtgcgcggtg agagcggcga gtttcgtgac gtgatcgaga aaactaggcg
     1021 ggagggcttt gtccgaatcc gggtcgatgg cgaaatcgtc gagctggggc ggccggaacc
     1081 gatccggctg aagaagggcg agcggcatac gatcgaagcg gtggtcgatc gattggtcat
     1141 gcgcgagggg attcgtgcgc ggttggcgga ctcggtggag acggcgctga agtggggcgg
     1201 gaacaagatg gtggtgctca gagaagggaa aacgtccaac gtccaatgtc caacgtccaa
     1261 cgtccaactg gaagaggatt gggaggaggt tcgttattct acggactacg gcaatccgga
     1321 gaccgacttc tcgttagggc aactgacccc gaggcatttt tcctttaaca gccatctcgg
     1381 cgcctgcccg gcttgtcacg ggctcgggac gcagttggtt tgcgatccgg agctgatggt
     1441 gacggacact tcgaaatcgc tcgccgaagg ggcggtggtc ccctggcggc gggggacgaa
     1501 acggatgcag gcgtattacc gcgggctgca aactgccctc gtccggcatt tcgatgtcgc
     1561 ggacaatatt ccgtttgagg atttgcccga gaagttcaaa gaggcgcttt atttcggcac
     1621 cggcgatcgc ccgatcgaga tgagcttcgg cggcaacggc cggggaacga agacggcgcg
     1681 accgtttgag gggttgctcc cgcaactgca gcggctctac ggggaaacgg aaagcgaatt
     1741 cacgcgcagc cggattcgcg cattcatgaa ccgcgcgccc tgcacgacct gccatggagc
     1801 gcggctgaag ccggaaattc tcgcggtcac gatcaatgcg cagagcggtc gggaagcgaa
     1861 catccatcag ttctgcgagc tgacgattgc ggccgcggca cagttcgtta ggcaactcga
     1921 tctctcggaa ccgcagcggg cgcttgtttc cgaagtcgtg cacgagattt cttcgcggct
     1981 cgaatttctt ctcgaagtgg ggctcgatta tctcaccctc aatcgcgaga gcggcacgct
     2041 ctccggcggc gaggcgcaac gcatccggct ggcgacgcaa atcgggtccg gtctggccgg
     2101 cgtgctctat gtgctcgacg agccgagcat cgggctgcat cagcgcgaca acgcgcggct
     2161 catcggcact ctgcgccggc tgcgcgatct cgggaattcg gtcatcgtgg tcgagcacga
     2221 cgaagagacg attcgcgcgg ccgatcatat tctcgatctc gggccggggg ccggtccgcg
     2281 cgggggcgaa atcgtggcgg caggaacgct ggaggaaatt ctgcaggcgc aaaattcgcc
     2341 gactgcggcc tatctctcgg gccgttccag aattgccatc cctcgccagc gggtcgcgcc
     2401 tcgctccaat gatccgtctt ccgacggctg gctgacgatc gtgggcgcga cggaaaacaa
     2461 tctccgaaac ctgaccgcgg cgattccgtt aggctgcctg acctgcgtga cgggcgtttc
     2521 cggaagcggg aaatcgaccc tgattgacga catcctgcgc cgcgcgctct tccgccattt
     2581 ttacaattcc aaagacaagc cgggcgaaca ccgcgcgctg cacgggctcg atcaaatcga
     2641 caaggcgatc atcattgatc aaagcgcgat cggccgcacg ccgcggtcca atccggtcac
     2701 ttacaccggt gcgtttacgc cgattcgcga gttgttcagc caactgccgg ccgcgcgcgt
     2761 gcgcggctac ggcgcggggc ggtttagctt caacgtcaag ggtggtcgct gcgaaaattg
     2821 tcagggcgac ggcctgatca agatcgagat gcatttcctg gccgatgcct acgctgtctg
     2881 cgaagtgtgc caggggaaac gctacaatcg cgagacgctc gagattactt ataaaggcaa
     2941 aaacgtggcc gacgtcctcg cgctgacggt tgatgaagcg tcccgtttct tccgcaacgt
     3001 gccgacggtg gcggataaac taagggcatt gcaggatgtg gggctgggat atttgcgcct
     3061 tggccaggcc ggcacgacgc tctccggcgg ggaagcgcag cgcgtcaaac tcgcgacgga
     3121 actggcgaag agggcgaccg gccgcaccct ctacatcctc gacgaaccga ccaccggatt
     3181 gcattttgcc gacatcgaaa agctcctgca agtgctgatg aaactgcgca acgcgggcaa
     3241 cacgctggtg gtgatcgagc acaatctcga gatgatcaaa tgcgcggact gggtgatcga
     3301 tcttggaccg gaaggaggcg agggcggcgg acgaatcgtc ggcgtcggca cgccggaagc
     3361 gatcgcagga atgaagggaa gtcacaccgg cgcgtacctg cggccgttgt taggcaaatg
     3421 aaccggaaat ttcctgccct cctgagcgcc gtcctcctct cgttagggtc ggcgagtcgc
     3481 gggcaacaga ccccggcgct ggaggcggcg gttgcgccgc tgagcgatgg cgtgccggaa
     3541 gtggcggtcg agcgactaaa aaccctgctc gcccagaatc cgccggccgc ggaacaactg
     3601 gtggcgcagc gcaagctagc agaggcgctc gtcgagatgg gtcggcccgc ggacgcgctc
     3661 gaggttgttg ccagccccgg cctggcgaaa gacccggagg ccgtcttctg gcacgcgcag
     3721 gccctcgcgg cgctcggccg ttgggcggaa gcgctcccgt tctaccaggc aatcgcgcgg
     3781 aatgaaaaat cgtcgcggcg gacggaggcg ctcttcggag aagcggaaat gttgcgcgcg
     3841 ctcggccaaa cggatgaagc cctgcccgtg ctcttaaaac tggagaatgc gccggaatgg
     3901 agtgtgcggg caaagttagg ggtagcccag ttgatgatcg aacggggcga tctcgcggcg
     3961 gctgatcgtc tcctccgcga gaccagaccg caacgcgcga ccgagcgcac cgaaaagcgc
     4021 tttctgtttg gccggctgag cctcgcgcag ggtcgcgccg aaaaggccat cgagactctg
     4081 accgtgattt tgaagaagcc ggcggatgtt tcacacccgc tgctgatcgc gactctcttc
     4141 gccctggccg acgcgcatct gaaggcgaag accccggaga tcggcgacga cgcgctcgag
     4201 gaatttatcg atcatcaccc taacgacccg gcgctgccca caatctttgc caagctcgac
     4261 gagctttacc ggatggagcg caagccgtcg acgagggagc tcgaacgctg gatgcgcgac
     4321 ccggccgagc cgcggcgcgc gctggcgcaa tggtatctgg cccgcagtaa acttcgctcc
     4381 ggcaaccggg agtcggcgat cgagctgctg acgcaagtgc gcgatagcgc ggtgcgcttg
     4441 ccgtcgttag gcgaggcgga tctggatttg gcgcgcctgc acctggcgaa ggatcagtgg
     4501 gagccggcga tcgccgccgc ggaagccgcc cgcgcgcaga attccgcgcc ggcgttcctc
     4561 gggcaggtcg aatggctgac ggcagaagcg aattatcgta gcgggcacct cgcgcaggcc
     4621 gcgcccattt acgagaaact ggcgctgcag gcgcccaccg tgtcgggcga cgcgctcttt
     4681 aacgcggccc tctgctggct gcggctcgat cgggccgagg agttctcggc ggactatcgc
     4741 aaaatcagca gcgaccccgc taagcaagcc gtccagggag aacttctcct cgagcaaggg
     4801 gccgcgcagg cggcgcaggg aaagggggaa gccggactga cgtttcaaca attcatccgc
     4861 gattttccgc aaagccagcg cgtctccgag gcgtgggtgg cgctggcgga actcgcgttc
     4921 catgctgcaa aaccggacat tgtaaccgcg cgcaaggact tggcacaggc gcgccaagga
     4981 catccgaccc aggccgcgct ggaacgcgct gattatctcc aaatctggct tgaagacgcg
     5041 acggaatcgc cggacgaaac gccggtcatc accgcagcca acaattttct ccagcaatat
     5101 cccgattccc gtttcatcgc cgaagtgcgg atgaaaatcg ccgaagccta ttttcgacga
     5161 caggattttg ccaacgcgca gacgcaattc gaattgctgg cgcagcaaaa gccggacggg
     5221 ccgctggccg agaaggcgct ctttttcgcc gcacgttcgg cgatgtcgag catgggcgcg
     5281 gagtcgctcg atcacgcgct cgcgttgctc gatcaggtgg tgaaactaaa cggtgacttg
     5341 aagtgggtcg cgcgcaatga agaggcggcg atcgagcgac gactgggaaa aaatcgagaa
     5401 gcgctggccc tttacgacga ggtgctgaag aacgatgcga aagccgcgga gaaccgggag
     5461 gcgctctgtg gaaaaggcga cattctctac gagatgggtg tgaccgatcc gcaaaattac
     5521 cggcgcgcga tcgaacttta cgatcaactt gcgaccgaat ccggtgtgtc ggcgcattgg
     5581 cgcaaccagg cggagttcaa aaaaggaaaa gcgctggaga aattgaatga tcaacccggc
     5641 gccctcgcga cttactacgg agtgatcgag aaaggcgccc ggcctgacgt gcagcacgaa
     5701 ttcttttggt tttataaagc gggtttcaac gccgcgcatt tgctggagga agcgaaggac
     5761 tggaaagccg cggtcgcggt ctaccgcaag ctggcggcgg tcggaggcac gcgcagcgac
     5821 gaggccaagg cgcggcttac gcagctccgc ctcgagcatt ttctctggga tgaataggcg
     5881 atttgggcgg ttcgaattcc gaagattcgt cttgccttcg ggcggctaac gggcgcagga
     5941 ttggaggatg aaaacaagaa gcggatggtt ccatctgcgc ggcttgtttc tgctcgcagt
     6001 gggtttcacc atggggatcg tattgagcgc aaccgcactc ggcggcccga atacggaccg
     6061 ggccgcgatg attgctcgct cccgcgtggc ccgaccccac atggcgcagg atcggcccgc
     6121 gcccaaaaaa gtggtgttct atgtgatgtc ctccgcctcg ccgatcccga aaccgatcgc
     6181 ctatttctcc gccggggtcc cgacgacgac gattccgatg gagattatcg gacgtggacc
     6241 ggagtcaggc ccccgttagg cgaaaggcgc ctgtcctccg cgcctaggat ttttcatcga
     6301 gccagggcat caggcggcgg aggcgcgcgc ccacatgctc gatcggttcg gcctgacctt
     6361 tatccaacag gcgctggtaa cgcttgcgtc ccgtcgccgt ttcgcgaagc cactcctgcg
     6421 caaatttccc gcggcggatc ttcaggagcg cttctttcat ccgtaatttg acgctttcgt
     6481 cgatgatctt tggaccgacc gagatgtcgc cccatttggc ggtctccgaa attcgctcac
     6541 gcatcccgct gataccggcg cgatgcatca aatccacgat gaacttcaac tcatgcaggc
     6601 attcgaagta ggccatctcc ggctggtaac ccgcctccac gagggtctca aatccggcct
     6661 ggaccagcgc gctcattccg ccgcataaga cggcctgctc ggcgaacaaa tcggcctcgg
     6721 tttcttcctg aaaagtcgtc tcaaggacac cggcgcgggt gccgccgatg gctttggcat
     6781 aagcgagcgc caactggcgt gcatgtccgt tcgcgtcctg ctgaattgca acgacacaag
     6841 gcaccccttt gccttcgacg aactgcgcgc ggaccatggc cccgagtccc ttgggcgcga
     6901 ccatgaccac gttgacttcg ttaggcggga cgatcgttcg gtaatgaacg gcaaagccat
     6961 gcgcaaagag gagcgtctgt cccgggcgga gatgcggcgc gatgtcgacc tcgtagattc
     7021 cggccatttt cgtgtcgggc agggcgagaa aaacgacatc ggcctggcgc acggccgcgg
     7081 ctgtcgtcgt taggcgcagt ccatcctggc gcgccgtgac ccgggaccgg ctcttgggaa
     7141 gcagaccgac gatgaccggg atcccgctgt cgcgcaggtt cagggcgtga gcgcgaccct
     7201 gcgcgccgta gccaacgacc gcgcaggttt tgtcgaggag cggcgcgaga ttgacgtctt
     7261 tatcggtgag aattctcgct aacataagcc gcggctggga gccgccgatg gtagcggtgc
     7321 gggctcgact gtcaatcccg cgcttcgagc ggcgggcgac taattcgcga gcgccgggga
     7381 ggggcggccg atcgcttcga ggcgcgtgac ctgagcgatg gcgtctttgg ccgcggcctt
     7441 ccgccccatt ttttccagga tatccgagag caaggcatat tggctgggcg cgtccggctg
     7501 ccgcgcgacc gcgcgcttct gcgcgcgata ggcatcagcc agacgatttt ggcggaggcg
     7561 catcagcgcg atcaaattca aagcatcgac ctcgtggagg tcgagccagc tcgccgtgcg
     7621 gaacgcttcg acagcgcgat ccgcatcgcc cgcttgcgca taaagccggc cgagggccag
     7681 ggccgcgccg tagtgccacc aatggctacg tgcaaaatcc gagacgagag caaaggcgtt
     7741 ggccgacttt ttcttttggc ggagcaactc ggcctccaga ctaacgattt cccaaacttc
     7801 cggataatcg gtgcgcgctt tttccaaagc ggcgagtgct tcatcgtcgc ggtgcgtggc
     7861 gtgcaggaga cgacccaaat tcaggactgc aatccaggcg cgcggataag tcttgctcgc
     7921 ctcgagggaa tgttttgccg aatctgcgaa aagagcttcg gcttccttcg ttttgccttc
     7981 ctcgtaatag acgtgagcca ggttgttgcg agccaccgga aaatcgggcg tgcctgcgag
     8041 cactttccgg aggagagctt cggccttggc gagcttaccg ctgcgggtat aaagctcggc
     8101 caggtttccg ctgacccgca tgctgtttcc cccggccgcc atcgtgcgct cgtaaaagac
     8161 ctcgggcgtg acccagtcgc tgctgcgcaa cgcgctgcgg atgctgaggc cgccggccgc
     8221 gacgcaggca aaagcggcaa gcgaggcgcg atagcgcgcc ggaaggtcga gcacacagcc
     8281 ggcgagaaaa agcagcaggc caacgctggg caggtaaagc cagtgctcgg cgcaagtcgc
     8341 attgagttcc acgatgttcg acgtgggtag gtaactgatg atgaaccaac tcgcgccgaa
     8401 gatgcgtagc gagcggccgt tcccgcgcca gcagcaacca gcgatgagga gaaacgcgaa
     8461 gatcaatccg gcgatcgaga gatactcggt cgcgacagaa tgctcccagg tggtgtggct
     8521 gcgatagttg tctccatcca ccaccgtgcg ttccatgtgc agattggacg ggaaaatcat
     8581 cagccgccca taatcgccca gcgcccgaag cattaacacc gcgcgcaccg gcgcacccca
     8641 gccttccgaa ggacccggtc cggatcgcgg cgggggcaac tgccgaagtc ccatgtaaag
     8701 agcaaacaga gcaacgcaaa cggcgaccga ccacgtcttg ccgcggaggc gaattttgtg
     8761 gtcgaagaac aaagtgtgga aaagaaaaaa gaccaaccaa aggccggccg tttcgcgtga
     8821 acagagcgcc aggagaccga ggagcgttgc cgcgagaaaa aggaggtatc ggccggttcc
     8881 cggcttcatc gcgcgcgcgc gaaagacgag ataccacgcg ctcgaagcga aaaggaaagc
     8941 gaggctatcg gcccgcccgg agatataatc gactgccgcg ctgtgcacgg gatgcacggc
     9001 ccaaaggaga gcgacgaaga acgccaggtc cgccaggaag ttcgaaccgt cccggagcca
     9061 ggccgcgtca cgcgccgctt gcagcaagcg cttgaccaaa aagtagagca tgaccgcgct
     9121 cccggcgtgc agcagcagat tggtgaggtg aaaaccgaag gtgttcgtat tccaaaaaaa
     9181 gtagtccgcc atgaacgaca ggttctgaat cggtcgatag tgcccggaga atgagtcggg
     9241 gaagagatag tgccggaaaa cctccagcac gaggagcgga ctcttgatga aggggctgtc
     9301 gcgcgcgaga tattcgtcat cccagaggaa agcgcccgag agggagggtg ttcgaaccac
     9361 aatggcgata agcaaaagaa acagggcacg ccacccatcg gaataaagga taggcgccat
     9421 gtgctgggag agcgtggctg caccggcgcg gaggtgcgtt ttggcggcgg atgaaagtgc
     9481 gttcaaattg aagccggaat cgaaccacat ccttcggagc agcgttgatg ccaatgcccg
     9541 cgttttcatg ggattgccca cgaagccaga aaacggagat ctgtgggcgt gtctttgcta
     9601 cggggtgtaa ggcgagaagt aggcagttcc cgccacgtcg gacaaagcgt tccaggtaaa
     9661 gagcggaatc ttaggcaggc tgtctacggt ctgcgggcca tagtcctgtc caaacatatc
     9721 ctggcccgtg ttgtagagga gagagttctg tttcatgtag gaggtccagg ccgtcaccgg
     9781 aacaaccgcc ccggttgcct tgctgttttc gatcgcgtat tgatccaccg cggaaacgat
     9841 caggcgaaga tcgttcagga tgcggcctgc ctggacgcgc ttgcgagccc ggagaaatcc
     9901 cggaatggcg agggcggata acaaggcgat gatcgcgacc acgaccatga gttcggtcag
     9961 ggtgaagccc catctgtcct taccgagtct gcttaccatt gcattccttg cagcaccctt
    10021 gatgccggtc ggcgtggttt ccgacattgc ctcccaaatg acaggacggg attcgatgaa
    10081 cggatagcac ttgctggagg gacgtgattg cgccgcggcg gacgtggctc cggcggtgga
    10141 ccagggcgct catactggag gcaaagatcg acttctctgg cgaccgaccg tgcgactcat
    10201 tgcctgccac ccccgaatcc tttcggggat ggcaggtgta tgaatcagac gtttcctgct
    10261 acggcccgta gggcgagaag aaggcggttc ccgcgacgtc ggacaaggtg gcccaggcgt
    10321 tggctgggac cttcgggagg ctatccacgc tctgagaacc gtacgcgtcg ccgaacatat
    10381 cctgccccgt attgtagagg atagagttcg ccttcatgta ttgggtccag gccgccgttg
    10441 ggacaatcgt gccggtaacc ttgctgtttt cgatcgcgta ttgatccact gcggagtcga
    10501 tcagacgaag atcgttcagg atgcggctcg cctgggcgcg cttgcgggcc cggagaaatc
    10561 ccgggacggc gatggcggct aacagtgcga tgatcgcgac cacgatcatg atttccacaa
    10621 gggtgaagcc cccgcggttt ttattgagtt tttgtaacat gctatttgct ttctatctgg
    10681 tttgtttgtt ttgattgcgt ccgccgttcc atcgcggatt gcctctttta ggagcagcgt
    10741 tgatgccaac ccgcctgatt ttaatgattt ccagagcttg gggcacatgt cccacggcgg
    10801 gcgcctgatg aaccttctca gtaaatccag ctcgctccgg gctgttccga ccggggttta
    10861 tctcttcctt ggcgtttttc tccttcgctt gatcgtcctc gtccggttga ccggcagcca
    10921 atttctgctg ccgaccgccg gggacatgca gttctacaac gaatgggctt tgcggattct
    10981 gcgcgggagc tggaccgagc acacagcctt ctacggtttg ccgctttatc cttttctgct
    11041 cgctgggatc tataagattt gcggctatag cccgtttgtt cccgggttgc tccaggcggg
    11101 gtgcgagggc ggtacagccg tcctcttgta ccaactgagc gcgttggttt ttcccgcacg
    11161 gggcgtggcg gattctgcgg aagagaagaa gccgtcgata aacgtcatcg gcctcggggc
    11221 ggtgatcggt tgggcattct ttcagccggc tcaggtctat tcagtcattc tgatgccgac
    11281 cgcgtggctg gtcttcgtct tctggtttgt cgtctggcag atcgtcaaac gccgggaagc
    11341 cccagcgctc tggaagcttc tgctcatggg aatgctgatt ggcttcaccg ccatgggtat
    11401 cgcaacgatc ttgttcctgg tcccgctcgt attcgcggcg cttttcctac gttgggccgc
    11461 ccctttttct tatcgggtcg ccggcacggt ggcggtgatc gccggcattt ttgtcggcgc
    11521 ttcccccgcc tggattcata acgccttcgt cgcgcgcgac ccggtgtttc tttcagccca
    11581 cagcggggtc aatttttgga tagggaacaa cccgatggcc aacggttatc cgaggtttcc
    11641 gcccggtctg catgctgggc agcaggcgat gctgcaggac tcgatcgcga ccgctgagaa
    11701 agcggcaggg caccctctca agcgctctaa ggtttccgcc ttctggtcgc aaaaagcccg
    11761 cgcctggatc cagggtcatc cgctggactg ga
//
LOCUS       DATMNS010000012        35368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1453177, whole genome shotgun sequence.
ACCESSION   DATMNS010000012 DATMNS010000000
VERSION     DATMNS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 35368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35368
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453177"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1518)
                     /gene="clpX"
                     /locus_tag="VNW28_00355"
     CDS             complement(238..1518)
                     /gene="clpX"
                     /locus_tag="VNW28_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HXA08398.1"
                     /translation="MARASNLTMCSFCGKSHAEVRKLIAGPGVYICDSCINVCKSILD
                     KELNEDARRQSSTMRVPKPVDIRRSLDQYVVGQDIAKKTLSVAVHNHFKRVLQSAPPT
                     DTSAAFQPDPFADVEIEKSNILLIGPTGSGKTLLAKTLAKILDVPFCIADATTLTEAG
                     YVGEDVENIVLRLLQNADYDVKRAEIGIVYIDEIDKIGRKTDNVSITRDVSGEGVQQA
                     LLKILEGSTCNVPPQGGRKHPHQEYIQVNTEKILFICGGAFVGLDKIVQKRIGNKTMG
                     FSSPSLEVEEAMRRESVRQVEPEDLLGFGMIPEFIGRLPVLSALDALTEDEMVMILTD
                     TKNAMIKQYTKLFAMEGVRLTVTKDALRALAAQAVTKGTGARALRSMLERIMLDIMYD
                     IPSREDIAEVTINRGVVEGKKLPLIRKKQDKDAA"
     gene            complement(1544..2257)
                     /locus_tag="VNW28_00360"
     CDS             complement(1544..2257)
                     /locus_tag="VNW28_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HXA08399.1"
                     /translation="MTTNRDSNYSQSVLVPMVVEQTGRGERSYDIYSRLLKDRIVFIG
                     TPIDDHIANLVIAQLLFLQMEDGKKDINIYINSPGGSVTAGLAIYDTMQFLTCDVTTY
                     CLGMAASMGAVLLSAGTKGKRFALPNSDIMIHQVSGGAQGQASDVERTVEYMFKLKKR
                     LIRILSQHTGKPEEQVKLDSDRDYYMTAPEAKAYGLVDEVIKSRKEAKLLEGAGSEKS
                     LNDRSTAVAEAALPQKVEE"
     gene            complement(2254..3600)
                     /gene="tig"
                     /locus_tag="VNW28_00365"
     CDS             complement(2254..3600)
                     /gene="tig"
                     /locus_tag="VNW28_00365"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXA08400.1"
                     /translation="MKVEVENQPHCVATLKIELPPEQVAKEWDAIANSFAAQARIPGY
                     RPGKAPRRVIEAKFRKDIQDELTKKLVSKSYHEAIAEKQLRVVSLTKLEDVEFGDDRS
                     MRFRATVVTAPEFELPDYKGIPLQLPDTKVTEAEVDAAVEQLREQSADFVGVPERGLE
                     LEDFAVIDFEGTIDGQPIAELVPAASKNLLGGKKFWLRLASDNFLPRFVEQIIGQKPD
                     ETRTVKVEFPADFPVAELVGKTAEYRVTLQEIKQRVLPPLDDAFAAKWMPEKTLPELR
                     HALEHQIEHEKGHELERAREGQVIKFLSERIQFELPPALLKHETRNALAELVQRNRER
                     GVPDEALKEKEEELIRVAGGLAAHRLKTNFILHRIAEQEKIQVTRADIDERIRHQAMH
                     HNVTPEKLRQDIEQHDGMNGLVEQVLLAKTIDFLGSNATIDSTRATPVPEAFAESK"
     gene            complement(3847..5091)
                     /gene="hutI"
                     /locus_tag="VNW28_00370"
     CDS             complement(3847..5091)
                     /gene="hutI"
                     /locus_tag="VNW28_00370"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HXA08401.1"
                     /translation="MNSIALLHVAQLVTLAGPERARIGAELSDLAIIENGGMLVRDGV
                     IAAIGASDEIARQLPQNVEVVDAGGRVVLPGFVDAHAHPVFGGNRVDEFEMRARGATY
                     EEIAASGGGIRSTVRKTRTASEADLLAQAQKRANWFLRCGTTTMEAKSGYGLSLEEEL
                     KILRVIRQLNETTAIEFVPTFLGAHAIPEEFRRAPEQYVALVIHEMLPCVAEEHLAES
                     CDVFCERGYFDLADSEKILIAAQEHGLRVRMHVDQLTNTGGAFLAARLRAATADHLEQ
                     TNDAEIAALAEAGVQPVLLPASVYALGKTRYPKARAMIEAGLAVVLATDFNPGSSPTP
                     SIPMVLSLAATQMKMTPAEGVTATTINAAYSLGRGDRIGSLEVGKAANFSVFDCADYR
                     EIAYWFGSPQTHAVFVKGRPAL"
     gene            complement(5176..6858)
                     /gene="ftcD"
                     /locus_tag="VNW28_00375"
     CDS             complement(5176..6858)
                     /gene="ftcD"
                     /locus_tag="VNW28_00375"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HXA08402.1"
                     /translation="MQKLIECVPNFSEGRDPNIIGQITAAIAAVDAVSLLDVDPGAST
                     NRTVVTFVGSPAAAVEAAFRGIQKAAELIDMRKQKGAHPRMGATDVCPFIPVSDVSWE
                     EAIECARSLGKRVAGELKIPVYLYEKAATDPSRSNLSIIRAGEYEGFFEKIKQTAWKP
                     DFGPAVFNARSGATVIGARDFLVAYNANLNTRSVRRANSVAFDVRENGRVQTEDGTPA
                     GKKVLDANGEPVRIPGMLKHVKAIGWYVEEYGIAQVSMNLTNIAETPLHAAFDACSEA
                     AIRRGLRVTGSEVVGMLPKKCLLEAGRYFLRKQQWSEGASEEDLIDLAIRSMGLSELK
                     PFEPKEKVIEYRIEAAAGKSLVKMNLRQFCNETLSDSPAPGGGSVAALMGALGASLGG
                     MVANLSAGKRGWDDQIAFFSEWAVKGQQLKDELLFLVDEDTAAFNKVMAAFGLPKDSA
                     EEKAARSKAIQKANQYAAEIPLKVMETASRSYELLAAMAERGNPASISDVGVGLLATR
                     ACIEGAAMNVRINLSGLKDEKVKTTLQEKMSRLSAESETRFKNARQTVEGKL"
     gene            complement(6876..8543)
                     /gene="hutU"
                     /locus_tag="VNW28_00380"
     CDS             complement(6876..8543)
                     /gene="hutU"
                     /locus_tag="VNW28_00380"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HXA08403.1"
                     /translation="MTGKRTIRAPRGNQLSCKGWQQEAALRMLMNNLDPDVAENPDQL
                     IVYGGTGRAARSWEAFDAIVRSLRELENDETLLVQSGKPVGIFRTHDEAPRVLIANSN
                     LVGQWSNYPEFNRLEKLGLTMYGQMTAGSWIYIASQGIVQGTFETFSAAGERHFGGSL
                     EGRLLVTGGLGGMGGAQPLAATMAGALCLGIEVDPTRIEKRLQSGYCDKIAHSLDEAM
                     RLLGEARGKMEAISVGLVGNCADILPEMVRRGIVPDVLTDQTSAHDALNGYVPNGMTL
                     AEALQLRAEKPDEYVDKSMAAMAQHVEAMLALQKKGAITFDYGNNLRTQAKKAGCPKA
                     FDIPGFVPEYIRPLFCEGKGPFRWVALSGDPADIARTDRLALELFPDNQILSRWMKLA
                     QKRIQFQGLPARICWLGYGERAEFGVALNELVRRGEVSAPIVIGRDHLDAGSVASPFR
                     ETEGMLDGSDAIADWALLNALVNTAAGASWVSIHNGGGVGIGYSQHAGMVVVADGTDN
                     AKRRLERVLTSDPGMGVIRHADAGYSRAIRFAKEQGVKIPMEPQARD"
     gene            complement(8540..10054)
                     /gene="hutH"
                     /locus_tag="VNW28_00385"
     CDS             complement(8540..10054)
                     /gene="hutH"
                     /locus_tag="VNW28_00385"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HXA08404.1"
                     /translation="MMQLSGRPLSLSEIARVALGQEPVEIDPGAHLAIHASRRVVEEI
                     VSRQAVVYGVSTGFGKLADVQISPGKLEQLQVNLVRSHSCGVGRPLSEAEVRAMMLLR
                     ANVLTLGFSGIRLQVIQLLVTMLNRNVCPVIPEKGSVGASGDLAPLAHLALSLLGEGE
                     AFFQGERMPSGQALQKAGLRPTDLQAKEGLALLNGTQAMHAVGGLALLRAKRLSRVAD
                     VAGAMTLEALKGTPVAFDERIHHARPHPGQQAVARHLLALLRESEIRESHAKDDPRVQ
                     DAYSLRCIPQVHGAVRGALAHCEDILAIESGSATDNPLVFTDNSDVLSGGNFHGAPLA
                     LAFDYAAIAMTDLMGISERRIDRLTNPDLSEGLPAFLAQHPGMQSGFMIAHVAAVALL
                     NEAKVLAHPASVDNLPTSAGKEDHVSMGMTGALKLRTIVEDAENVLAIELLAAAEGLE
                     FRRPLKAGTGVERAFAALRGLAPRVDEDRALSPDIERVASAIRSGKFDSAHEDL"
     gene            complement(10511..10972)
                     /locus_tag="VNW28_00390"
     CDS             complement(10511..10972)
                     /locus_tag="VNW28_00390"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXA08405.1"
                     /translation="MQTSAPIIRISVRDQRIELVAGDSVLSSYPISTSRFGLGSEEGS
                     MKTPLGKFKIGEKIGAGVPSGTVFRGRVPLGPDEAPPPTDDRILSRILWLDGLEEHNA
                     NTRQRYIYVHGTNHEDEIGQPASHGCIRMRNADLIALFDQVPLGAQVVIET"
     gene            11004..12002
                     /locus_tag="VNW28_00395"
     CDS             11004..12002
                     /locus_tag="VNW28_00395"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXA08406.1"
                     /translation="MPKNYHVAIVGATGAVGIELLRVMERRNFPVADLRLLASARSAG
                     KKLEFRGEDFPVAALQHDSFAGIDLAFFSAGAGTAKQFAPIAQRTGAVVIDNSSAFRM
                     DADVPLIIPEINPEDIRRHLGMIANPNCTTAVALMALYPLHGTFGLRRVFASSYQAVS
                     GSGAQAIEELRRQIEASVDGGPALAHVYPHPIAFNVLPHVDSFLESGYTKEEMKMQNE
                     GRKIMHLPDFRASVTCVRVPVYRAHSVALNAEFERPVSVESARAVLAKAPGLDLVDEP
                     LADRYPTALAAAGKDHCQVGRLRSDCAFENGLALWVAGDQLLKGAALNAVQIAELL"
     gene            complement(12056..13567)
                     /locus_tag="VNW28_00400"
     CDS             complement(12056..13567)
                     /locus_tag="VNW28_00400"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HXA08407.1"
                     /translation="MKRFLLALTMVGFLLPLAPRAQAETEVSLNFFYDNLSPYGNWIE
                     VGDYGYCFQPNVAVDSPDWRPYADGYWAYTDVGWTWVSYEDFGWATYHYGRWTDLADY
                     GWVWIPGYEWGPAWVSWRTGGDYIGWAPLPPGGDVYEGSAITGQVDVQFDIGPLYYNF
                     VDVRYIGEPVLRGRIFPPARNVTFINQTVNVTNITYNNSTVYNYGPNYNRLNEYSTRP
                     IQRLSLQRETAVGGNFGQGGKHGNFSRVNGNQLVVVAPAIQKAPQSLAPRQVKAKVPQ
                     PKVEHGWQGIANRQQVQEEMKKENAQHVLPPTFQPQKGRKAEDVTRGQTAAAQPNEAM
                     PANERNQQGEKAARQGTKNGPQQNAGAAVGAANENANPDAQASQFEQPNGQNAAREQK
                     TRLAVQQRAEQQRKAQEENRARQPNAVKPAQSPVRHAERPSQAQRPQRPEQTEPPKPA
                     RPQSPPQERPAQVPNHSQRVQQPQPPPGEQNPNAPHKPKKKNPQDQKPDQPGQ"
     gene            complement(13649..15178)
                     /locus_tag="VNW28_00405"
     CDS             complement(13649..15178)
                     /locus_tag="VNW28_00405"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HXA08408.1"
                     /translation="MKNILKFLLLVLVLSAGVSLLYDYQLRSGRLSLTTRETPEKYTL
                     AQNVVVDPKAIASLASLSDERRKLVAGVIPSVVSVKTTKRIQRRQGLDPFDFFRGNMG
                     QFRGPADEAMVQNSLGSGVIVTNEGHIITNNHVVDQVDEIEVVLSDGRTRKARLIGAD
                     AQLDLAVLKVDEPGVKPLKLGDSDAMQAGDFVLAVGNPFGFEETVTDGIISSRGRPNR
                     ADDFGDFLQTNAAINPGNSGGPLINLRGEVIGINTAIISRSGGSQGIGFAIPSNTVRM
                     ALESLLKRGRIIRGYLGIETTGMQVGPEGARDDSGVVVAAVVPNSPAAAAQIQRGDII
                     QKFDGHDVHNIAELRRLVSQVELDKKVDVQVARSGKPVTVTAKIKEQPENYGLARALP
                     PAQPAPSAPNEPDAGEQDQPPGGGGALDSIEVRELTPQLAQTLGVPPSVRGVVVAQAG
                     SGSGAGVLRAGDVIEAVNQEPVTSVQEFEQAIQSLDPSQPQVLSVCRQRSRSFVVIKP
                     H"
     gene            complement(15178..17880)
                     /locus_tag="VNW28_00410"
     CDS             complement(15178..17880)
                     /locus_tag="VNW28_00410"
                     /inference="COORDINATES: protein motif:HMM:NF028500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXA08409.1"
                     /translation="MSLTSADLLSEVAWIPRPRSTPLRRLGLETVGDLLTHYPRRHED
                     RREFVGFPREESEQPICLCGEVVKTQLRRFGGWKKIFEVTLQESDAHALSQPLVCRWF
                     NLHYIQKMIVTGQRLVVFGKPRLRGQRLCMDHPEFEVIENDEEISIHFRRITPIYPAT
                     EGLSQRVLRGLIYRLLEECDDHSLQVDLPAKLSTALTARSWREIHFPESEETLARARE
                     AIVLREFFAMQMLLASRRARTRARSGAAHSGPGELLERFLRALPFDLTNAQVRVIEEL
                     RADLAAPIPMNRLLQGDVGSGKTVVALAAMLLAVEAGFQTALMAPTQILAEQHYAVLQ
                     GWLEPLGVRVSLRTGARKEDPLPLFAAANAQTPGSARVSRAGDGVSPSRTFLKAEESD
                     EARYHRRCLPHFEKPWAIYAVTIGTKARRCLSPAARGVALDALLHFHNQRYELFTACV
                     MPDHVHLLIQPWPKGDDDKGNVVFWSLSELLRSVKSFSAHRINKLENTAGSMWAREQF
                     DRYIRSDRDLEEKFRYIVGNPWNSEVARPGEDYPWLWTQEGAARKESSSRWHSATSTR
                     DACATQQPPPAAGQIFVGTHALLYEPQGLENPGLVVIDEQHKFGVAQRARLTSSEPAP
                     DVLVMTATPIPRTLTMSLYGDLDVSTIDEMPAARGRIVTAARDEKKLPEVLEFLRREL
                     EAGRQAYIVYPLIDESERLEAKAASAEFEKWHERLQPFTCELLHGRIPAPEKQAIMER
                     FRRGLTSVLVSTTVIEVGVDVPNATVMLIENSERFGLAQLHQLRGRIGRGEHKSYCIL
                     LRSDPSAETTAKLAVLEKTSNGFAVAEADWEMRGPGDLLGTAQSGLPALKLGNLRSDA
                     KLMQRARATAMEILRDDPKLARPENKRFRCLVVEEEGKTFSHVS"
     gene            complement(17877..19586)
                     /gene="recJ"
                     /locus_tag="VNW28_00415"
     CDS             complement(17877..19586)
                     /gene="recJ"
                     /locus_tag="VNW28_00415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HXA08410.1"
                     /translation="MKRERRWVLAPRNSPAGDAKFSDTFCALPCIAILLQRKGLLSDE
                     QATEFLHPRLRSLSDPFLLPDMPAAVDRILRAITAGERIVLYGDYDVDGVTSLALLNE
                     MLCAYGARPTPFLPSRMEEGYGLSREGIERCWQTHRPQLLIAVDCGTSSSAEIANLRA
                     RGVDVIVLDHHEPKGDLPDCVAVVNPKAVGNSAFCYLCSVGIVFKLCHALLKTRPLPD
                     FDLRQRLDLVALGTVADIVPLEAENRVLVHHGARQLALRRLLGVSKLMDVAAVRPPIV
                     AEHIGFRLGPRLNAAGRLTTAEKALQLLLTADLAEATALAGELDGQNRERQSVEHAIV
                     EAAERKIAEQNLSAAPAIALGERGWHPGVLGIVASRLARNYHRPTIVVGFDQTGLGKG
                     SGRSIPGLSLVAALGECAGLLEKFGGHEMAAGLTIREENFATFTGLFQERCRARLSDE
                     QLEPRLHLDGELMLSDLNWELLRWHELLQPFGNGNPQPLFLVRAVEPAAPPKILKEKH
                     LLLRLRQNGHQQRAIFFGGAESPLPEPPWDVAFRLSMDEYEGERRLQMQVEALRAAAP
                     LES"
     gene            complement(19746..20513)
                     /locus_tag="VNW28_00420"
     CDS             complement(19746..20513)
                     /locus_tag="VNW28_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HXA08411.1"
                     /translation="MPVPASLRLGCVRYRNALPLIHGWGGAVRFDHPSALCRQLAAGE
                     LNVALVSSFEYLRNPIYSIVDGLALASDGPVFSVILAHLGAVEALREVIVDPASETSI
                     NLLRCLLGEQGVAVRFVPEGEISEERGQLLIGDQAILFRQQSGRRHQFLDLGAEWKER
                     MKTPFVYALWLIHPDYPEKPEIAGALRSLARRNLEHLETLIAAQPEQDRAFCDFYFRR
                     CLRFSFGAEEKEGFKMFGELCAGQKLLSPGLPALRLA"
     gene            complement(20515..21669)
                     /gene="mqnE"
                     /locus_tag="VNW28_00425"
     CDS             complement(20515..21669)
                     /gene="mqnE"
                     /locus_tag="VNW28_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HXA08412.1"
                     /translation="MKLSLIPPTLRPIADKIERNERITDEDALQLYRSRDLNALGIMA
                     SVVRERKNGNVATYVLNRYINYSNICVLSCQFCAFAAKKRDPHAFENSIDEIVVAVQE
                     ALLTGITEVHMVGGLHPSLDKQWYLDLLTRLRALDPQLHLKAFTAIEIRHLARRIFRT
                     SIAETLRVLREAGLNSLTGGGAEIFDPGVRDELCRGKESAAEWLEVHRIWHQMGGRST
                     CTMLYGHIESHEHRVDHLRQLRELQDETYGFTGFIPFAFESQTTILAHIKPATAFDQL
                     RNLAVSRIYLDNIDHLTAYWVSMGLPLAQVALAYGVDDLHGTIMREKIFHMAGVTTPE
                     EQTVEMLRHVIREAGRDPVQRDSYYSHIPAAKSDLPASAAPRAEGELVCA"
     gene            complement(21821..23317)
                     /locus_tag="VNW28_00430"
     CDS             complement(21821..23317)
                     /locus_tag="VNW28_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXA08413.1"
                     /translation="MPATTAGSQTATGPTVTNSITESGGVGVREFQGDDVGQVLRLLA
                     RQAKINMVVSEAVTGTVTMRLEDVTALQAVAIIVKAKGLFMDQIDNVYYIKTGAERTA
                     EPTESDNFIFSYARAKDVAPLLAAQLSSKEAPQVDDRTNTVFFRETRSNIDNIRKMLV
                     QIDKPTKQVMIEARLVEVTANPKQSYGINWAGVVGSSTTPQTVKYGAIGQTTQTTTID
                     PTGIPTTDLAKTSHSWFGNLIPEQFAILSVPQMSLTLRALNEDSDAEFLANPRVVTAD
                     NQQAKIEIIRNQPVPQLNFNEQTATAVFGGFQDKKFGNTLVVRPSVNKDDFITLMVKP
                     EISNKVADATFVFAGATVASPIIDTRTLDSNVLIKSGDTLAIGGLLQDEVTKAHNKVP
                     IMGDIPVLGYFFQEKLNARTKRNLLVFVTPTILAQRYGTGLEDQVSGLTHSGEEYADP
                     NGWRNNAKGAVRLVPTSNRQIASDYPKPGVVPAPTKMRYMSTADERDN"
     gene            complement(23850..24620)
                     /locus_tag="VNW28_00435"
     CDS             complement(23850..24620)
                     /locus_tag="VNW28_00435"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08414.1"
                     /translation="MTYCLGMLCRKGTVFLSDSRTSAGMDNITVRTKMRVFEKPGDRV
                     ICIMSSGNLSLTQATLALIDDDLHLNESHPARKHLMNRQTMYETVRYVGSKVREVEQR
                     DRAALEADGFDFNINLIVGGQIAGLAPEVHSIYPQGNSIHASRDCPFLQIGESKYGKP
                     ILDRGFSYESTLMDALKFGIISIDATMKSNVAVGPPIDILCYKTDSLEVKMRTRLEEN
                     DPYLLEIGRKWQDGIVRLVRQMPAADFSKPALGFATAA"
     gene            complement(24617..25132)
                     /locus_tag="VNW28_00440"
     CDS             complement(24617..25132)
                     /locus_tag="VNW28_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXA08415.1"
                     /translation="MLEVGQAFTHKSAIWQAETEFLAAVTLRMRHFVFRWAITTVAVM
                     VAASIISGIRYDNVASLLGAALLLGILNAFLRPILLLLAAPLIILTLGLFIFVVNALL
                     LYLVNDLVHGFHVDGFWSAFWGAILIGLVSWMLSAFFRGSDGRVYPLTHHAQMRRVQG
                     RVVGSSKPGES"
     gene            25139..25528
                     /locus_tag="VNW28_00445"
     CDS             25139..25528
                     /locus_tag="VNW28_00445"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HXA08416.1"
                     /translation="MVKNSKKSKETAIPESKTPIPSPAAGNGDRAAHSAEPVGAKRAA
                     AKSVKPARNTKSTSKRQAGAVSTRKPRAGAERKDRAGKVTISEEQIRLRAYFIAERRM
                     QHGIPGDSAHDWLEAQRQLQKEAKKRA"
     gene            complement(25541..25957)
                     /locus_tag="VNW28_00450"
     CDS             complement(25541..25957)
                     /locus_tag="VNW28_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA08417.1"
                     /translation="MAKADAKRVLIVDDDVAILRIIRDTLQNFLHWEVETSPKPEYGF
                     ELALKKDYDLMIFDFSMPLIDGSLLFLLISKVYENASPPRKVPPLLLVSGQGSDERAQ
                     ELLKEARVHGFLPKPFTINRLLEKVKECFPEARAIA"
     gene            26030..26263
                     /locus_tag="VNW28_00455"
     CDS             26030..26263
                     /locus_tag="VNW28_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08418.1"
                     /translation="MNSPENELAGALRERLALIADEASRREPASHLLKLKSVSDKIAT
                     LERALAPPVDPQLAHFLERCSYDKALALLEARL"
     gene            complement(26356..26967)
                     /gene="rpsD"
                     /locus_tag="VNW28_00460"
     CDS             complement(26356..26967)
                     /gene="rpsD"
                     /locus_tag="VNW28_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012010904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXA08419.1"
                     /translation="MARYTGPKTKIARRYGVPLFGPSKSLERKNYPPGMHGPKGSRRK
                     QSDYAIALGEKQKLRFQYGLMEKQFRRIFEAALRRRGVTGETLLQLLETRLDNVVFRL
                     GLANTRSAARQLVSHGHVQVNGRNVNISSFNVKAGDEITVKNRPKSRQLAQRNMELTQ
                     IAPVPDWLTVDRDALTGKVAHIPSREEMQPMVNEQLIVELYSR"
     gene            complement(27006..27377)
                     /gene="rpsK"
                     /locus_tag="VNW28_00465"
     CDS             complement(27006..27377)
                     /gene="rpsK"
                     /locus_tag="VNW28_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXA08420.1"
                     /translation="MIKAKGSKNVYSGFAHVLSTFNNTIVTITDLQGNVIGWSSAGKV
                     GFKGSRKSTAYAAQMVAQDASRQAMGHGLKEVEVLVRGPGAGRESAVRALQAIGLDLT
                     VIRDVTPVPHNGCRPPKQRRV"
     gene            complement(27685..28080)
                     /gene="rpsM"
                     /locus_tag="VNW28_00470"
     CDS             complement(27685..28080)
                     /gene="rpsM"
                     /locus_tag="VNW28_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXA08421.1"
                     /translation="MPRLLGVEIPADKRIEASLTYIYGVGPSTAKRILEQTNIDPNLR
                     AKDLTPQQINEIIQMITSNKLPIEGDLRRELQSNLKRLQAINCYRGIRHRRGLPVRGQ
                     RTSTNARTRKGPRKTVGVIRNKDAKTGKV"
     gene            complement(28090..28206)
                     /gene="rpmJ"
                     /locus_tag="VNW28_00475"
     CDS             complement(28090..28206)
                     /gene="rpmJ"
                     /locus_tag="VNW28_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HXA08422.1"
                     /translation="MKVRPSVKRLCEACKIVKRKNVVRVICNKNPRHKQRQG"
     gene            complement(28244..28462)
                     /gene="infA"
                     /locus_tag="VNW28_00480"
     CDS             complement(28244..28462)
                     /gene="infA"
                     /locus_tag="VNW28_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXA08423.1"
                     /translation="MARKDAIEVEGTVVELLPNTMFRVELPNGHGVLAHISGKMRMHF
                     IRILPGDKVMLEVSPYDLSKGRITFRHR"
     gene            complement(28435..29211)
                     /gene="map"
                     /locus_tag="VNW28_00485"
     CDS             complement(28435..29211)
                     /gene="map"
                     /locus_tag="VNW28_00485"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXA08424.1"
                     /translation="MIPIKSAREIDKMRLACRTASDVLERVGELIRPGISTKEIDQAA
                     ADLMQDAGVRSAFLGYRLGHRVFPGNICISLNDEIVHGIGSQRRIQYGDIVKLDIGVV
                     QDGWVGDTAATIPVGMIDERIEKLLAVTESALQRAIGHAASGERLGDLCAAIEDEAMR
                     HHFSVVREFVGHGVGRKLHEEPQVPNYGKRGSGPRLKPGMTLAIEPMINLGGSGVRLL
                     EDGWTVCTSDGLPSAHFEHTVLITKEEPEILTWRAKTQLR"
     gene            complement(29208..29864)
                     /locus_tag="VNW28_00490"
     CDS             complement(29208..29864)
                     /locus_tag="VNW28_00490"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HXA08425.1"
                     /translation="MKRRIVLLGAPGSGKGTQAEMITRHFAIAVTSPGTILRRERDLE
                     TPLGLEADEISKQGGLVPDDIIVHLIEDWLNLHGRDGFVFDGFPRTVKQAERLNEILQ
                     KRKAALDLALWLEVSEQTVRERIASRLQCRRCGFTTSLTSAHFADRPVCPYCDGPLIR
                     RDDDDAAVLQTRLVEFKAKTEPLLSFYEKGDALHRIDGNRDRESVFADIVALIEERVP
                     "
     gene            complement(29865..31373)
                     /gene="secY"
                     /locus_tag="VNW28_00495"
     CDS             complement(29865..31373)
                     /gene="secY"
                     /locus_tag="VNW28_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXA08426.1"
                     /translation="MVSAFLNSVKIPELRRRILFTLAVIVIVRLGAAVTTPGVNPAVL
                     QDWFRTSSQTAGGGVAALFNLFSGGALENCAVFSLGIMPYISASIMLQLLTAVIPSLS
                     RLSREDGGRQKIMQYTRYATVVLCIFQGYLLAVSFQHPESYHTMLPGITETTARLGIP
                     LVDNLGWSFRIITVLTLTAGTLFLMWLGDQITDRGIGNGISLIITIGIVARLPAALAQ
                     AWKTFVPSAETGTSQVNPAILVLMVAFLFIVIAAVIAVTQGVRKITVQYAKRVVGRKM
                     YGGQTQYMPLKVNYAGVMPIIFAWALLLFPTTIVGLAFKNSRTAQEIANMLSTGWLHY
                     VVLAAMIFFFSYFWVATQFQPAQIADDLKKYGGYIPGVRPGKPTADFLDFTMTRLTFA
                     GAIFLTMIAILPSLLSQGLHVPVITAQFFGGTSLLIIVGVILDTMRQVETHLIQRHYD
                     GFLRKGRIRGRAFDNRSAYGRGEAAQQGTLMWLYVGIAVLVIGGVAIFLYGR"
     gene            complement(31548..31976)
                     /gene="rplO"
                     /locus_tag="VNW28_00500"
                     /pseudo
     CDS             complement(31548..31976)
                     /gene="rplO"
                     /locus_tag="VNW28_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
     gene            complement(31976..32536)
                     /gene="rpsE"
                     /locus_tag="VNW28_00505"
     CDS             complement(31976..32536)
                     /gene="rpsE"
                     /locus_tag="VNW28_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003328273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXA08427.1"
                     /translation="MAYAGNQGRRSDSRTTDRTPPARGDTTEKVVFINRCAKVVKGGR
                     RFSFSALIVAGDHDGKVGCGFGKANEVAEAIRKASEAARKSMEKMSLHENTIPHEAIG
                     EFGGGRVLLRPASPGTGVIAGGGVRAVVEAVGIRDVLAKSLGSSNHANVVKATIEALR
                     SLRRRDEILKTRGLRSNNVDETRQQS"
     gene            complement(32543..32908)
                     /gene="rplR"
                     /locus_tag="VNW28_00510"
     CDS             complement(32543..32908)
                     /gene="rplR"
                     /locus_tag="VNW28_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXA08428.1"
                     /translation="MATINRKVIRGRIHDRIRRKVSGTAERPRLCVYFSGKHVYAQVV
                     DDETGRTLVSAGTTEKELGGNGKHAANRATAEKVGKLIAERLLAKKIDKAVFDRGGFL
                     YHGKVKALADAAREGGLKL"
     gene            complement(32911..33447)
                     /gene="rplF"
                     /locus_tag="VNW28_00515"
     CDS             complement(32911..33447)
                     /gene="rplF"
                     /locus_tag="VNW28_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXA08429.1"
                     /translation="MSRIGNKAVEIPAKVKVNVAGSKVAVEGPKGKLDWTLPKGIQAN
                     VADNQVSLVRDAETRSVKALHGLSRALIHNMVVGVSEGFTKQLEIEGVGFKAAVQGKN
                     LNLSLGFSHPVLFAIPTDIKITVTDNTKIAIHGADKKTVGQVAADIRRFYPPEPYKGK
                     GVRYAGEQIRRKEGKTVQ"
     gene            complement(33460..33858)
                     /gene="rpsH"
                     /locus_tag="VNW28_00520"
     CDS             complement(33460..33858)
                     /gene="rpsH"
                     /locus_tag="VNW28_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXA08430.1"
                     /translation="MSTVTDPIADLLARVRNSNHAQKLEMFVPYSKIKSEVVRILKEE
                     GYISDYALDTTAAHPRIKITNKIANRTSAITGLKRVSRPGLRRYVAADEIPRVLGGMG
                     TSILSTSRGIMSGREAKKQKVGGELLAYVW"
     gene            complement(33864..34436)
                     /gene="rplE"
                     /locus_tag="VNW28_00525"
     CDS             complement(33864..34436)
                     /gene="rplE"
                     /locus_tag="VNW28_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXA08431.1"
                     /translation="MKNEFYREYKERVMPALREVHGYKNEHQIPRLEKVVINTSVGSQ
                     SDVKQALEEAKAELALITGQKPAETRAKKSISNFKLRKEQAIGAKVTLRGEHMYEFME
                     RLIKAALPRIRDFRGVSPKGFDGHGNYTLGVSDQSIFPEVELDKIKRNIGFDVTIVTT
                     ARTNEEAKSLLSEMGMPFSDLAKRPAAQAA"
     gene            complement(34450..34746)
                     /gene="rplX"
                     /locus_tag="VNW28_00530"
     CDS             complement(34450..34746)
                     /gene="rplX"
                     /locus_tag="VNW28_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXA08432.1"
                     /translation="MNRLKHHVKKGDQVEVISGNFRGSTGKILQVISRKDRVLIEGVR
                     MIKKHLRKSQDNPSGSIAEREGPIHISNVKLVEGGEKETKKKSKAKATKEKKTK"
     gene            complement(34743..35111)
                     /gene="rplN"
                     /locus_tag="VNW28_00535"
     CDS             complement(34743..35111)
                     /gene="rplN"
                     /locus_tag="VNW28_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXA08433.1"
                     /translation="MVYLRSRLDVADNSGARMATMIGVIGKGSAQSAGIGDVISANVK
                     EASPTGTVKKGEVVRAVLVRTRQPIRRDDGSLLRFDNNAIVIIDKDLNPRGTRIFGPV
                     ARELRERNFMKIVSLAPEVL"
     gene            complement(35130..35351)
                     /gene="rpsQ"
                     /locus_tag="VNW28_00540"
     CDS             complement(35130..35351)
                     /gene="rpsQ"
                     /locus_tag="VNW28_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016660790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXA08434.1"
                     /translation="MGEVISSGMNKTIIVRTVTRVPHAKFGKIVKQQKKFYAHDEENK
                     AKSGDTVRIMETRPLSKLKRWRLVEVVQK"
BASE COUNT         6613 a        10957 c        10469 g         7329 t
ORIGIN      
        1 gtcgaaattt tcttccagcg cttcgcgaat cgccaaacca tccgggacgg aaatctgacg
       61 ttcgacgcgc aagcgaagcg gaaaaatctc gcgcgcgata aaggtgagat gcgtgttgag
      121 gacgtcaccg aggaggattt cggtgccggt gttgatgacg acgactcgca ttggttgaga
      181 cagaaacgtt caacgttgag cgttggacat ttaacgctag gcgcgaagcg ccctccttta
      241 cgccgcgtct ttgtcctgct tcttgcggat gagcggaagc tttttccctt cgacgactcc
      301 gcgattgatc gtcacctcgg cgatatcttc gcggctcggg atgtcataca tgatgtcgag
      361 catgatccgc tcgagcatcg agcgcagcgc ccgggcgccg gtccccttgg tcaccgcctg
      421 cgcggcgagc gcacggagcg cgtcttttgt gaccgtcagg cggacaccct ccatcgcgaa
      481 gagcttcgtg tactgcttga tcatcgcgtt cttcgtatcg gtcaggatca taaccatttc
      541 gtcctcggtc agcgcatcga gcgcgctgag gaccggaaga cggccaatga attccgggat
      601 catgccgaag cctagcaaat cttcgggctc cacttgccgg acggattcgc gccgcatcgc
      661 ctcctcgact tcgagcgacg gactgctgaa gcccatcgtc ttgttgccga ttcgtttctg
      721 cacaattttg tcgagcccga cgaacgcgcc gccgcagatg aagaggatct tttccgtgtt
      781 cacctggatg tattcctggt gcggatgttt gcgcccgccc tgcggcggga cgttgcaggt
      841 gctgccttcc aatattttca gcagcgcctg ttgcacaccc tcgcctgaga catcgcgggt
      901 gatgctcacg ttgtcggtct tgcggccgat cttgtcgatt tcgtcgatgt agacgatccc
      961 gatctccgcg cgctttacgt cgtaatcggc gttctgcagc aggcgcagaa cgatgttctc
     1021 gacatcttca ccgacgtagc ccgcttcggt cagagtggtc gcgtcggcga tgcaaaatgg
     1081 gacgtccaga attttggcga gcgtcttggc gagcagcgtt ttgcccgagc cggtcggtcc
     1141 aatcaagagg atgttgctct tctcgatctc aacatcggca aaaggatcgg gctggaaagc
     1201 agcggaggta tcggtcggcg gggcgctttg caggacgcgc ttgaagtgat tgtggactgc
     1261 gaccgaaagc gtctttttcg cgatgtcctg accgaccaca tattggtcga gcgagcgccg
     1321 gatgtcgacc ggcttcggca cgcgcatggt cgacgattgc cgccgggcgt cttcgttcag
     1381 ctccttgtcg aggatgcttt tgcagacgtt gatgcagctg tcgcaaatgt agacgccggg
     1441 acccgcaatc agcttccgaa cctccgcgtg gctcttgcca cagaaggagc acatggtgag
     1501 gttcgaggcg cgggccatat tcgttccctc ggtaaagcag ttcctattcc tcgaccttct
     1561 gcgggagggc cgcttccgcg accgccgtgg accggtcatt caggctcttt tcggaccccg
     1621 cgccctcgag gagtttggct tccttgcgcg acttgatgac ttcgtctacg agaccgtagg
     1681 ccttggcctc gggggcggtc atgtagtaat cgcgatcgga atcgagtttc acctgctcct
     1741 ccggtttgcc ggtatgctga gagaggatgc ggatgagccg ctttttgagc ttaaacatat
     1801 actcgacggt gcgctcgacg tcgctcgcct ggccttgggc gccgcccgag acctggtgaa
     1861 tcatgatatc ggaattgggc agggcaaagc gcttcccctt cgtcccggca ctaagcagga
     1921 ctgcgcccat actcgcggcc atgccgaggc agtaggtggt cacgtcgcag gtcaaaaact
     1981 gcatcgtgtc gtagatcgcg agccccgcgg tcactgaacc gccgggcgag ttgatataaa
     2041 tgttgatgtc cttcttcccg tcctccattt ggagaaagag gagctgggcg atgacgagat
     2101 tggcgatgtg atcgtcaatc ggcgtcccga tgaaaacaat ccggtctttc aataaccgcg
     2161 agtaaatgtc gtaactgcgt tcgccgcggc cggtctgctc cacgaccatc ggaacgagca
     2221 cggattgaga gtagttggag tcgcgatttg tggtcatttt gattcggcaa aagcttcagg
     2281 cacgggcgtt gcacgcgtgc tgtcgatggt agcattcgac ccgaggaaat caattgtctt
     2341 ggccagcaag acctgctcga cgaggccgtt catgccgtcg tgttgctcga tgtcctggcg
     2401 cagtttttcc ggggtcacgt tgtgatgcat cgcctggtga cggatgcgct cgtcgatgtc
     2461 cgcgcgggtc acctggattt tctcctgctc cgcgatgcga tgcagaatga agttcgtttt
     2521 cagccgatgg gcggcgaggc cgccggccac gcggatgagt tcctcttcct tttccttgag
     2581 cgcttcgtcc ggcaccccgc gttcgcggtt gcgctgcacc agctcggcca gagcgttgcg
     2641 cgtttcgtgt ttgagcaaag ccggcgggag ctcgaactgg atgcgctcac tgagaaactt
     2701 gatcacctgc ccttcgcggg cccgctccag ctcatgacct ttttcgtgtt cgatctgatg
     2761 ctcgagcgcg tggcgcaatt ccggcagcgt cttctccggc atccacttcg cggcaaaagc
     2821 atcatcgagc ggaggcaaaa cgcgttgctt gatctcctgc agggtgacgc ggtattcagc
     2881 cgtcttcccg accagctccg cgaccggaaa atcggcggga aattccacct tcaccgtccg
     2941 cgtttcgtca ggcttttgtc cgattatctg ctcgacgaag cggggcagaa aattgtccga
     3001 ggccaggcgc agccaaaact ttttgccgcc gaggagattc ttgcttgctg ccggcaccag
     3061 ctccgcgatc ggctggccgt cgatcgtgcc ctcgaagtcg atcaccgcga aatcctccag
     3121 ctccagcccg cgctccggga caccgacaaa atcggcggac tgttcccgca actgctcgac
     3181 ggccgcgtcc acctcggcct cggtgacttt cgtgtccggc aactggagcg ggattccctt
     3241 gtaatcgggc agctcgaatt ccggcgcggt tacgaccgtg gcgcggaaac gcatcgagcg
     3301 atcgtcgccg aattccacat cttcgagctt cgttagggaa accacccgaa gctgcttctc
     3361 ggcaatcgct tcgtgatagc tcttggagac gagttttttg gtcaactcgt cctggatgtc
     3421 cttgcgaaac ttcgcctcga tcacgcgccg cggcgctttc ccggggcgat aacccgggat
     3481 ccgggcctgc gcggcgaagc tgttcgcgat cgcatcccac tccttcgcca cctgctccgg
     3541 cggcagctcg attttaagcg tggcgacgca gtgcggttga ttttcgactt ccaccttcat
     3601 gtagaaggca cgttacgggt ggcaagagtg cggcgcaaac ctggtagccg tcgtcccatg
     3661 ggtccgcgcc gtcgcccccc gtggggggcc tgtggccgtt gccctgcggc cctgtagccc
     3721 gctgccctat cgccccgtag ccgttgccct gtgcccctgt agccgttgcc ctgtgggcaa
     3781 cgagagagtg gagtcgcgat actatcttca gttcgccctt ctctctcctt cgctccggcg
     3841 aagcgtctac aacgccggcc gacccttaac gaagaccgcg tgcgtctgtg gagagccgaa
     3901 ccagtaggcg atttcgcggt aatcggcgca gtcgaagacg ctaaaattgg ccgctttccc
     3961 aacctcgagc gaaccgatcc gatcgccgcg gcccagactg tacgcggcgt tgatcgtcgt
     4021 cgccgtcacg ccttcggccg gcgtcatctt catttgtgtc gcggccagcg agagaaccat
     4081 cggaatcgaa ggcgtgggcg atgaaccggg attgaaatcc gtcgccaaca cgacggccaa
     4141 accggcctcg atcatcgcgc gcgctttcgg ataacgggtc ttcccaaggg catagacgga
     4201 agcaggcagc aggaccggct gaacgcccgc ctcggccaga gccgcgatct cggcgtcgtt
     4261 cgtttgttcc agatgatcgg ccgtcgccgc gcgcaaacgc gccgcgagaa acgctccgcc
     4321 ggtattcgtt agctgatcga catgcatccg gacacgcaac ccgtgctcct gcgccgcgat
     4381 cagaattttt tccgaatccg cgaggtcgaa gtagccgcgc tcgcaaaaga cgtcgcagct
     4441 ttccgccaga tgttcctcgg ccacgcaggg cagcatttcg tgaatgacga gcgcgacata
     4501 ttgctcgggc gcgcggcgaa actcttccgg aatggcatgt gcgccgagaa acgtcggcac
     4561 aaattcaatc gcggtcgtct cgttcagttg acggatgacg cgcagaatct tcagctcttc
     4621 ctccagggac aggccgtagc cggatttcgc ttccatcgtc gtcgtcccgc agcgcaggaa
     4681 ccagttggcc cgtttttgcg cctgcgcgag aaggtccgct tcgctggccg tgcgcgtctt
     4741 gcgcaccgtc gagcgaattc ccccaccgct ggcggcgatc tcttcgtaag tcgcgccccg
     4801 cgcccgcatc tcgaattcat cgacccgatt cccgccgaaa accggatggg cgtgcgcatc
     4861 gacaaaaccc ggcaggacaa cgcggccgcc agcgtcgacg acttcgacat tttgaggcaa
     4921 ttgcctcgcg atctcgtccg atgcgccgat cgcggcgatc acaccatccc gcaccagcat
     4981 cccgccattt tcgatgatcg cgagatcgct gagttcagcc ccaatccgcg cccgttccgg
     5041 gccggcgagc gtgaccagtt gagcaacgtg taataaagcg attgagttca ttgatcatcc
     5101 cgagcggagt cgggggatcc ggctgcgaaa gcctaaaggt gactttacgg gatccctcgg
     5161 cttcgctcgg gatgactaca gcttcccttc caccgtttgg cgagcgttct tgaaacgggt
     5221 ttcggattca gcgcttagcc tgctcatttt ttcctgcagc gtcgtcttca ccttctcatc
     5281 tttcaggccc gacaaattga tccgcacatt catggcggcg ccttcaatgc aggcccgcgt
     5341 cgcgagcagg ccgacgccga catccgagat cgatgcggga tttcccctct ccgccatcgc
     5401 cgccagcagt tcgtaggatc gactcgccgt ctccatgacc ttgagcggaa tttccgcggc
     5461 gtattggttc gccttctgga tcgccttgga ccgcgccgct ttttcttcgg cggaatcctt
     5521 cggcaaaccg aaagccgcca tcactttgtt aaaagccgcc gtgtcttcat cgaccaggaa
     5581 aagcaattcg tccttgagct gctgcccttt cactgcccat tcgctgaaaa atgcgatttg
     5641 atcgtcccag ccgcgtttgc ccgcggaaag gttggctacc attccgccta acgacgcgcc
     5701 aagcgcgccc atcagggccg caaccgagcc gccgccggga gccggcgaat cgctcagggt
     5761 ttcgttgcaa aactggcgca ggttcatctt cacgagcgat tttccggcgg ccgcttcgat
     5821 cctgtattcg atcacttttt cctttggttc gaacggcttc agctcgctca agcccatgga
     5881 gcgaatcgca aggtcgatca agtcctcttc cgacgcgccc tcggaccact gctgcttgcg
     5941 cagaaaatag cgcccggcct cgagcaggca tttcttcggg agcatgccga ccacttccga
     6001 gccggtgaca cgcaaaccgc gcctgatggc cgcttcgctg caggcatcga aggccgcgtg
     6061 caaaggcgtc tccgcgatgt tggtcaggtt catcgagacc tgggcgatgc catactcctc
     6121 gacataccag ccgatggctt tgacatgttt caacattccc gggatgcgca ccggttcacc
     6181 gttcgcgtcg agcacttttt tgcccgccgg cgtgccgtct tccgtctgca cccgtccgtt
     6241 ttcgcgcaca tcgaacgcga ccgagttcgc gcgccgcacc gacctggtgt tgaggttggc
     6301 gttgtaggca acgagaaaat cgcgcgcgcc aatgacggtc gcgccggatc gcgcattaaa
     6361 caccgccggg ccgaaatccg gcttccaggc ggtttgtttg atcttctcga aaaaaccttc
     6421 gtattcgccg gcgcgaatga tggagagatt cgagcgggac ggatcggtcg ccgctttctc
     6481 atagaggtaa accggaatct tcagctcccc ggcgacccgt ttgcctaacg atctggcaca
     6541 ttcgatcgct tcctcccagc tgacgtcgct caccgggatg aacgggcaaa catcggtcgc
     6601 acccatgcgc ggatgcgcac ccttttgttt gcgcatgtcg atcagctcgg cggccttttg
     6661 aatcccgcga aaggcggcct cgacggccgc ggccgggctc ccgacaaaag tgaccacggt
     6721 gcgattggtg ctcgcgccgg ggtcgacatc gaggagcgaa acggcatcga ccgcggcgat
     6781 cgcggcggtg atttgcccaa tgatgttcgg gtcgcgtccc tcgctgaaat tgggaacgca
     6841 ttcgatgagc ttttgcattg ttaggctctt tcctatcaat cccgcgcctg cggctccatc
     6901 ggaatcttca cgccttgttc tttcgcaaac cgaatggcgc gggaataacc agcgtcggca
     6961 tggcggatca cgcccatccc gggatcactc gtcaggactc gctcgagacg acgtttggcg
     7021 ttatcggtgc cgtcggccac caccaccatc ccggcgtgct gcgagtagcc gattccgacg
     7081 ccgccgccgt tgtgaatgga aacccaggaa gcacccgccg cggtgttgac gagtgcgttg
     7141 agcagcgccc agtcggcgat cgcgtcactg ccgtcaagca ttccctccgt ctcgcggaaa
     7201 ggcgacgcga ccgagccggc gtcaagatgg tcgcgcccga tcacgatcgg cgcgctcact
     7261 tcgccgcgcc taacgagttc gttcagggcg acgccaaact ccgcgcgttc cccgtagcca
     7321 agccagcaaa ttctcgccgg caatccctga aactggattc gcttttgcgc cagcttcatc
     7381 cagcgcgaga gaatctggtt gtccggaaac aactccagcg cgagacgatc ggttcgtgcg
     7441 atatcggccg ggtcgccact cagcgcgacc caacggaacg gtcctttccc ttcgcaaaaa
     7501 agcggccgga tgtactctgg aacgaagcca ggaatatcaa aggccttcgg gcagccggct
     7561 ttcttggcct gcgtccggag gttgttgccg taatcgaaag tgatcgcgcc ttttttctgc
     7621 aacgcgagca ttgcctcgac atgctgcgcc atcgcggcca tcgatttatc gacatactcg
     7681 tccggctttt ccgcgcgcaa ctggagcgct tcggccagcg tcattccgtt aggcacataa
     7741 ccgttgagcg cgtcgtgcgc gctcgtctgg tcggtaagaa cgtcgggcac aatcccacgc
     7801 cgaaccattt ccggaaggat gtcggcacag ttgccgacaa ggccgaccga gatcgcctcc
     7861 attttcccgc gcgcttcccc gagcaaacgc atcgcttcgt cgagcgagtg cgcgatcttg
     7921 tcgcagtaac cgctctgcaa tcgcttctcg attcgcgtcg gatcgacctc gatcccgaga
     7981 cagagcgcgc ccgccatcgt cgccgcgagc ggctgggcgc cgcccattcc gccgagccca
     8041 ccggtcacga gcaagcggcc ctcgagcgag ccgccaaaat ggcgttcgcc cgcggcggag
     8101 aaagtttcga atgttccctg cacgatgccc tggctggcga tgtaaatcca cgacccggca
     8161 gtcatttggc catacatcgt taggccaagc ttttcgagcc ggttaaattc cggatagttc
     8221 gaccactgcc cgacgaggtt cgagttggcg atcaacaccc gcggcgcttc gtcgtgggtc
     8281 cgaaaaatgc ccaccggttt gcccgactgg acgagaaggg tttcgtcgtt ttcgagctcg
     8341 cgcagcgaac gcacgatcgc atcgaacgcc tcccagcttc gcgccgcgcg gcccgtcccg
     8401 ccgtaaacga tcagctgatc tggattttcc gcgacgtcgg gatccagatt gttcatcagc
     8461 atccgcaggg ccgcttcctg ctgccatcct ttgcagctca gctggttgcc acgcggggcg
     8521 cggatcgttc tttttccggt cataaatctt cgtgtgcgct gtcgaatttc ccgctgcgga
     8581 tggcgctggc gacccgctcg atatccggcg agagagcccg atcttcatct acccgcggtg
     8641 cgaggccgcg caacgccgcg aaggcgcgct ccacaccggt gccggctttg agcggtcggc
     8701 gaaattccaa gccctcggcc gcggcgagga gctcgatcgc cagcacgttt tccgcgtcct
     8761 ccacaatcgt gcgcaacttg agcgcgcccg tcattcccat cgagacgtga tcttctttcc
     8821 cggccgaggt cggcagatta tcgacgctcg ccggatgcgc gaggaccttg gcctcgttga
     8881 ggagcgcgac ggccgcgacg tgcgcgatca tgaagcccga ctgcatcccc gggtgttgtg
     8941 cgagaaaagc cggcaacccc tcgctcaggt cgggattcgt taggcgatcg atccggcgtt
     9001 cgctgatgcc catcagatcg gtcatcgcga tcgccgcgta atcgaaggcg agggcgagcg
     9061 gcgcaccgtg aaagtttccg ccggaaagga cgtcgctgtt atcggtgaaa acaagcgggt
     9121 tatccgtcgc cgagccggac tcgatcgcca ggatatcctc gcaatgggcg agcgcaccac
     9181 ggaccgcgcc atgcacctgc gggatgcaac gcaggctata cgcgtcctga acccgagggt
     9241 catctttcgc gtgcgactcg cgaatctcgc tctcgcgtaa gagcgcgagc agatgtcgcg
     9301 cgacggcttg ctgaccggga tgcggccggg cgtggtggat gcgctcgtca aaagcgacgg
     9361 gtgtgccttt aagcgcttcg agagtcatcg cgccggcgac atccgcgacg cgcgagagac
     9421 gcttggcccg gaggagcgcg agaccaccga cggcgtgcat cgcctgggtg ccattgagga
     9481 gggcgaggcc ttctttcgcc tgtagatccg ttgggcggag cccggctttt tgcagggcct
     9541 ggccgctcgg catgcgttca ccttgaaaga atgcctctcc ttccccgagc aacgaaagcg
     9601 cgagatgcgc caagggtgcg agatcaccgc tcgcgccgac cgaacccttc tccggaatca
     9661 ccggacagac gtttcggttt agcattgtca cgaggagctg gatcacttgc agacgaatcc
     9721 cgctgaagcc gagggtgagg acgttggcgc gcaggagcat catcgcccgc acttctgcct
     9781 ctgagagcgg acgtccaacg ccgcaggaat gactccgcac gagattgacc tgaagctgct
     9841 ccagtttgcc gggcgagatc tgaacgtcgg ccagctttcc aaagccggtg ctgacgccgt
     9901 aaaccaccgc ctggcgcgag acaatttcct ccaccacgcg gcgcgaagcg tggatcgcca
     9961 gatgggcgcc ggggtcgatc tcgactggct cctggccaag cgcgacgcgg gcaatctcgc
    10021 tcagtgacag tggacggccg cttagctgca tcattggtgc gaaatattac gagaagcgtt
    10081 ccataaatcc acccgctctc ttcgaggacg tttgtcggcg actccctctg ggggcgcgat
    10141 aagctgggga gttggttgaa gggctggcac tgacattccc cggctccgca gcctccggct
    10201 cggagacagc gcagcgcctg ccggacggaa aaaggccagg ccgtagcaac gacctggcct
    10261 ttcggttaaa gttgtccgtc gggggcgagc cgccggtgaa aactatttcg cggatgtcgt
    10321 cgctggcgct ggcgccaccg tctccttttt ctgcgcgcaa gcacccaggc tcaacacgcc
    10381 ggccgcgacg gccacgatca tgatcatttt gactaatttc actataaact cgcctccttc
    10441 cttagcaggt tgctgccgac tagttaggcg gggaaaccag cgcttgcaac gaaattctcg
    10501 gccttttttt ctacgtctcg attacgacct gcgcgcccag tgggacttgg tcgaaaagcg
    10561 cgatcaaatc ggcgttcctc atccgaatgc aaccgtggct ggctggctgt ccgatctcat
    10621 cttcgtgatt ggtgccgtgg acataaatgt aacgctggcg ggtattggcg ttgtgctcct
    10681 cgaggccgtc gagccagaga atgcgcgaga gaatccggtc atcggtcggc ggcggcgctt
    10741 cgtccggccc aagcgggacg cggccgcgga agaccgtccc actgggcaca ccagccccga
    10801 tcttttcgcc gatcttgaat ttcccgagcg gcgtcttcat gctgccttcc tcggaaccga
    10861 ggccgaaacg cgaggtcgaa attggataac tcgacaggac ggaatcgccc gcaacgagct
    10921 cgatgcgctg gtcgcgcacg gaaattcgga tgatcggcgc ggacgtttgc atctccaggc
    10981 gcgggcgata gactgcgctc gcgatgccga aaaactatca cgtcgcaatc gtcggcgcga
    11041 ccggcgcggt tggaatcgaa ctgctcaggg tgatggaacg gcggaacttt ccggtggcgg
    11101 acctgcggct gctggcctcg gcacgttccg ccgggaagaa attggaattt cgcggagaag
    11161 attttcctgt cgccgcactc cagcacgatt cctttgcggg catcgacctc gcctttttca
    11221 gcgccggcgc gggcacggca aaacaattcg cgccgattgc gcagcgcacc ggcgcggtcg
    11281 tgatcgacaa ctcatccgcg ttccggatgg acgccgatgt cccgctgatc atcccggaaa
    11341 tcaatcccga ggacatccgc cggcatcttg gaatgatcgc gaatccgaac tgcacaaccg
    11401 ccgtcgcgct gatggcgctt tatccgctgc atggcacgtt tggtttgcgg cgcgtttttg
    11461 catccagtta ccaggctgtc tccgggagcg gcgcgcaggc gatcgaggaa cttcggcggc
    11521 agattgaagc cagcgtggat ggcgggccgg cgctggcaca cgtttatccc cacccgattg
    11581 ccttcaacgt tctcccgcac gtcgattcct ttctcgaaag cggttacacg aaggaagaaa
    11641 tgaaaatgca aaacgaaggg cgcaagatca tgcatctgcc tgattttcgt gcttcggtca
    11701 cgtgcgtgcg ggtgccggtt tatcgggccc attcggtggc gctgaatgcc gaattcgagc
    11761 gcccagtttc ggtggaatca gcgcgcgccg tcctggcgaa agcacccggg ctcgacctgg
    11821 tcgacgagcc gctagccgat cgctatccga ctgcgctcgc ggccgctggg aaagaccact
    11881 gccaggtggg ccggctgcgg agcgattgcg cgttcgagaa tgggctcgcg ctctgggtgg
    11941 ctggcgacca actcctgaaa ggcgccgcac tcaacgcagt ccaaatcgcc gagctattgt
    12001 gaaaaagcgg ccccgcgatt tctcccgggg ccgctgattt cggacctcga tttcgttact
    12061 gccccggctg gtcgggcttt tggtcctgcg gatttttttt cttcggcttg tggggcgcgt
    12121 ttggattttg ttctcccggc ggcggctgag gctgctgcac tcgctgcgaa tggttcggca
    12181 cctgcgcggg acgctcttgc ggcggcgatt gcggacgagc gggcttcggt ggctcggtct
    12241 gctctggtcg ctggggcctt tgcgcctgcg agggacgctc ggcgtgcctg acaggcgatt
    12301 gcgcaggctt cacagcattc ggttgccgag cccgattctc ctcttgggcc ttgcgttgtt
    12361 gttcggcccg ctgttgcacg gctagccgcg ttttctgctc tcgggcagcg ttttgcccgt
    12421 tcggctgttc gaattggctg gcttgcgcgt ccgggttcgc attttcattc gccgcgccga
    12481 ccgccgcacc ggcgttctgt tgagggccat tcttcgtgcc ctgacgcgcg gctttttcac
    12541 cctgctgatt tctttcgtta gccggcatcg cctcgttggg ctgcgccgca gcagtctggc
    12601 cgcgagtgac gtcttcagcc ttgcggcctt tctgcggttg gaaggttggc ggcagaacgt
    12661 gctgtgcgtt ctccttcttc atttcctcct ggacctgttg ccgattcgcg atgccctgcc
    12721 agccatgctc gactttgggc tgcggcacct ttgccttcac ctgcctgggc gcgagcgact
    12781 gcggtgcttt ctggatcgcc ggcgccacca ccacgagctg gttgccgttc acccgactga
    12841 agttcccatg ctttccacct tgcccgaagt ttcctccgac cgccgtctcg cgttgcagag
    12901 agagacgttg aatcgggcgg gtcgaatact cattcaaccg gttgtagttg ggaccatagt
    12961 tataaaccgt cgagttattg taggtaatgt tggtgacgtt cacggtctga ttgataaacg
    13021 tgacgttgcg ggcgggcggg aaaatccggc cgcgcagcac gggttcaccg atgtagcgca
    13081 cgtcgacgaa gttgtaatag agcggcccga tgtcgaattg gacgtctacc tgaccggtga
    13141 tcgcgcttcc ctcgtaaacg tcacctccgg gcggcagcgg cgcccaaccg atgtaatcgc
    13201 caccggtccg ccaagagacc caggccggcc cccactcgta accgggtatc cagacccagc
    13261 cgtaatcagc caggtccgtc cagcgaccat agtgataagt ggcccaaccg aagtcttcgt
    13321 acgagaccca ggtccagccg acatcggtgt aggcccaata gccatccgcg tagggccgcc
    13381 aatcggggct gtcgaccgcc acgttaggct ggaaacagta gccgtagtcg ccgacttcga
    13441 tccagttccc gtaaggcgag agattgtcgt aaaagaaatt aagcgagacc tcggtctcgg
    13501 cctgcgcccg cggggcaagc gggagaagga aaccgaccat cgttagtgcg agtaagaatc
    13561 gtttcatggt gtttgatgat gtgaaccttg gacctgattg gtccaaccgt tattcactgg
    13621 ttcggaaacc ggcccttggg cgtgctcgtt agtgcggctt aatgaccacg aaggaccggc
    13681 tgcgttgacg gcaaaccgaa agcacctgcg gttggctggg atcgagcgac tggatcgcct
    13741 gttcgaattc ctgcaccgac gtgaccggtt cctggttgac ggcctcgatc acgtcgccgg
    13801 cccgcaacac tccggcgccg ctccccgagc cggcctgggc gacgaccacc ccgcggacgc
    13861 tgggcggcac gcctaacgtc tgcgcgagct gcggggtcaa ttcccgcact tcgatcgaat
    13921 ccagagcgcc gcctccgccc ggcggctgat cctgttcacc cgcgtcgggt tcgttaggcg
    13981 cagatggcgc cggctgcgcc ggcggaagcg cccgcgccag tccatagttc tccggctgtt
    14041 ccttaatctt cgccgtaacc gtgacgggct tgccgctgcg cgcgacttgc acgtcgacct
    14101 tcttatccaa ctccacctgt gagacgaggc gtcgcagctc cgcgatattg tgcacgtcgt
    14161 ggccgtcaaa tttttgaata atgtcgccgc gctggatttg cgcggccgcc gcgggcgaat
    14221 tgggcacgac cgccgccacc accaccccgg aatcatcgcg cgcgccctct ggcccaactt
    14281 gcatcccggt cgtctcgata ccgagataac cgcggataat ccggccgcgc ttcaaaaggc
    14341 tttcgagcgc catgcgcacg gtgttcgacg gaatcgcaaa cccgatcccc tgggacccgc
    14401 cgctccgcga aataatcgcg gtgttaatgc cgatgacttc gccgcgcagg ttgatgagcg
    14461 gaccgccgct gttgccggga ttgatggcgg cgttggtctg caggaaatcg ccgaaatcgt
    14521 cggcgcggtt ggggcggccc cgggagctaa taatgccgtc cgtcaccgtt tcctcaaagc
    14581 cgaacgggtt cccgaccgcg agcacgaaat ccccggcctg catcgcgtcg gagtcgccga
    14641 gcttgagcgg tttgaccccg ggttcatcca ccttcaatac cgcgagatcg agctgcgcat
    14701 cggccccgat gaggcgggcc ttgcgggtcc ggccgtcact caggacgacc tcgatctcgt
    14761 ccacctgatc gaccacgtga ttgttcgtga tgatatggcc ttcgttcgtc acgatcaccc
    14821 cggagccgag cgaattctgc accattgcct cgtccgccgg gccccggaat tgacccatgt
    14881 tgccgcggaa aaaatcaaac ggatcgagac cctggcgtcg ctggatccgc ttcgtcgtct
    14941 tcacgctcac caccgaaggg atgacacccg cgaccagttt ccgtcgctcg tcgctcaacg
    15001 aggcaaggct cgcgatggct tttggatcga ccaccacatt ctgcgcgagg gtgtatttct
    15061 ccggcgtctc tcgcgtggtc aagctgagcc ggccgctacg caactgataa tcgtaaagca
    15121 gcgaaacccc cgcgctcagg acgagaacca acaaaaggaa tttcaggata tttttcatta
    15181 gctgacgtgg gagaaggttt tgccctcttc ttcgacaacc aaacaccgga aacgtttatt
    15241 ttccggccgc gcgagcttcg gatcatcgcg caagatctcc atcgccgtgg cgcgcgcccg
    15301 ctgcatcaat ttcgcatccg aacgaaggtt gcccagtttt aatgcaggca gaccgctctg
    15361 cgccgtcccg agtaaatcac ccggtccgcg catctcccag tcggcttcgg ccaccgcgaa
    15421 accgttgctc gttttctcca gcacggcgag tttcgcggtg gtttccgcag aggggtcgga
    15481 gcggagcaaa atgcaatagg atttgtgctc gccgcgccct attcggcccc gcaactgatg
    15541 gagctgcgcc aggccgaagc gctcggagtt ttcgattagc atgacggtgg cgttcggcac
    15601 atcgacgccc acctcaatca cggtggtgct gaccagcacg ctcgttaggc cacggcggaa
    15661 gcgctccatg atagcctgct tttccggcgc aggaatgcgc ccgtggagca actcgcaggt
    15721 gaaaggctgc aggcgctcat gccatttctc aaactcggcc gaggccgctt tcgcctccag
    15781 tctctcgctc tcgtcaatca gcggatagac gatgtaggcc tgccgcccag cctccaactc
    15841 acggcgcaaa aactccagca cttcgggcag tttcttctcg tcgcgcgcgg cggtcacgat
    15901 tctcccgcgc gcggccggca tctcatcgat cgtcgagaca tcgagatcgc cgtagaggct
    15961 catcgtgagc gtccgcggga tgggggttgc agtcatcacg agcacatcgg gagccggctc
    16021 gctgctcgtt aggcgcgcgc gctgcgcgac gccaaacttg tgctgctcat cgatcacgac
    16081 aagtccggga ttctccaacc cctgcggttc ataaagcagc gcgtgggtcc cgacaaagat
    16141 ctgccccgcc gcgggcgggg gctgctgggt agcgcaagcg tcccgcgtgc tggtcgcgct
    16201 gtgccagcgc gacgaacttt ccttgcgcgc cgcaccttcc tgcgtccaga gccacggata
    16261 atcctcgcct ggccgagcca cctccgagtt ccagggatta cccacgatat agcgaaattt
    16321 ttcctccaga tcgcgatccg agcggatata gcgatcgaac tgttcccgtg cccacatgct
    16381 cccggccgtg ttctcgagtt tattgatccg atgcgccgag aatgatttca ccgaacgcaa
    16441 caattcgctc agggaccaga aaaccacgtt gcctttgtca tcatctcctt tgggccatgg
    16501 ttggatcagc aggtggacat gatcaggcat gacacacgcg gtaaacaatt cgtagcgctg
    16561 gttgtggaaa tgaaggagtg catccagagc cacaccgcga gcagcgggcg acaagcaacg
    16621 gcgggctttg gtgccgatcg tcaccgcgta gatcgcccat ggtttttcga aatgaggaag
    16681 gcatcgacga tgataccgcg cctcgtctga ttcctccgct ttcagaaaag ttcgcgacgg
    16741 tgagacaccg tcgccagcac gcgagacgcg tgcgctaccc ggagtttgtg cattcgcagc
    16801 cgcaaagagc gggagcggat cctcttttcg cgcaccggtt cgcaacgaaa cccgcacgcc
    16861 taacggctcg agccatcctt gcagaacggc gtagtgctgc tcggccagaa tctgcgtcgg
    16921 ggccatgagc gccgtttgga agccggcttc aaccgcgagc agcatggccg cgagcgcgac
    16981 cactgtcttg cccgagccga cgtccccttg cagcaaccgg ttcattggga tcggggcggc
    17041 gagatcggcc cgcaactcct cgatgacgcg cacctgggcg ttcgttaagt caaagggcag
    17101 cgcgcgcagg aaacgttcga gcaattcccc cgggccgctg tgcgccgcac cgctccgggc
    17161 tctcgtgcgg gcccgccgcg aggcgagcag catctgcatg gcaaagaatt cccgcagcac
    17221 gatcgcttcc cgggcccggg cgagcgtttc ctcgctctct ggaaaatgaa tctcgcgcca
    17281 ggatcgcgcg gtgagcgcgg tgctgagttt ggccggcaaa tcaacttgca ggctgtggtc
    17341 atcgcattcc tccaaaagac ggtaaatgag cccgcgcaag acacgctggg aaagaccttc
    17401 ggtcgcgggg tagatcggag tgatgcgccg gaaatggatc gatatttcct cgtcgttctc
    17461 gatcacctcg aactccggat gatccatgca gaggcgctgc ccgcggagac gcggcttgcc
    17521 gaagaccacc agacgctgtc cggtgacgat catcttctgg atgtaatgca ggttaaacca
    17581 acgacaaacc agcggctgac tgagcgcgtg cgcgtcgctc tcttgcagcg ttacctcaaa
    17641 gattttcttc cagccgccaa agcgcctgag ctgcgtcttg accacctcgc cgcagagaca
    17701 aatgggctgc tcgctttcct cgcgcggaaa accgacgaac tcgcgccggt cctcgtgtcg
    17761 ccgcggataa tgggtgagca gatcgccgac tgtctccaga ccgagccgac gcaacggggt
    17821 gctccgcgga cgcggaatcc aggccacttc acttaataaa tccgcgctcg ttaggctcat
    17881 gattccagcg gcgcagccgc gcgcagcgcc tcgacctgca tttgcaggcg ccgctcgccc
    17941 tcgtattcgt ccatgctgag gcggaaggcc acgtcccacg gcggctcggg cagcggactc
    18001 tccgccccac cgaagaagat cgcgcgctgc tgatggccgt tttgtcgcaa acgaaggagc
    18061 agatgtttct ccttcaaaat tttcggaggt gcggccggct cgactgcccg gacaagaaaa
    18121 agcggctggg ggttgccgtt cccgaatggt tgcagtagct cgtgccagcg gagcagctcc
    18181 cagttcagat cgctgagcat gagctcaccg tccaagtgca gtcgcggctc aagctgctcg
    18241 tcggacaacc gtgcacgaca acgttcctga aacagacccg tgaatgtcgc aaagttttct
    18301 tcgcgaatcg ttagtcccgc ggccatctcg tgtccgccga atttctcgag cagaccggca
    18361 cattcgccga gcgcggccac gagcgacaat cccggaatgc tacgcccgct gcctttcccg
    18421 aggccggttt ggtcgaagcc gacgacgatt gttggccgat gataattccg cgcgagccgc
    18481 gacgcgacga ttccaagaac gcccgggtgc catccgcgct cgccgagcgc gatcgccgga
    18541 gccgcagaga gattctgttc ggcaatcttc ctttccgcag cctcgacgat tgcgtgctct
    18601 acactctggc gctcgcggtt ttgtccgtcc aactcaccgg ccaaggcggt cgcttcggcg
    18661 agatccgccg tcaggagaag ttgcagggcc ttttccgcgg tcgtgaggcg accagccgcg
    18721 ttcaaacggg gaccgagccg gaagccgata tgctccgcga cgatcggcgg ccggacggct
    18781 gcgacgtcca tcagtttgct cacccctaac aatcgacgaa gggcgagttg acgcgcgccg
    18841 tgatggacaa ggacgcgatt ctccgcctcc agcggcacaa tatcggcaac cgtcccgagg
    18901 gcgacgaggt cgagccgttg ccgcaggtcg aagtcgggga gcgggcgagt tttcagcagg
    18961 gcgtggcaga gtttgaagac gatgcccaca ctgcaaagat agcagaaggc ggagttgccc
    19021 acggccttgg gattcacgac ggcaacgcaa tccgggaggt cgcctttcgg ctcgtgatga
    19081 tcgagcacga tgacgtccac gccgcgcgcg cgaaggttgg caatctcggc gctcgaagaa
    19141 gtcccgcaat cgaccgcgat gaggagttgc ggccgatgcg tttgccagca gcgctcgatt
    19201 ccttcgcggc tcagtccgta gccttcctcc atccgcgagg gcaggaaggg ggtgggccgg
    19261 gccccgtagg cgcagagcat ttcgttgagc aacgcgagcg aggtgacgcc atccacgtcg
    19321 taatcgccgt agagaacaat tcgctcgcca gcggtgatcg cgcgcaggat ccgatccacg
    19381 gcggcaggca tgtccgggag caggaatggg tcgctcaagc ttcgtaagcg cggatggaga
    19441 aactcggttg cctgttcatc gctgaggagg cctttgcgtt gcaagagaat ggcgatgcag
    19501 ggtagagcgc agaaggtgtc ggagaatttg gcgtccccgg cgggactgtt ccggggcgcg
    19561 aggacccagc ggcgttcgcg tttcatttca acaaatcgtt aggccaggga tccggccggc
    19621 caggggacgt gaagtttcgc atcggtctca agataatcga aagcatggcg tctgcgttgc
    19681 ccgtggccaa ccccgtcgaa cgcgatctga gttccgggac cccaaagcct cgtcgcgccg
    19741 gctggttagg cgagccggag cgcgggcaac ccgggtgaaa gcaacttttg cccggcgcat
    19801 aattcaccga acattttgaa accctctttt tcctctgcgc caaagctaaa ccgcaggcat
    19861 ctccgaaaat aaaaatcgca gaaagcccga tcctgctccg gctgcgccgc gatcagggtc
    19921 tcgagatgct cgaggtttcg cctggccaac gatcgcagtg ccccggcgat ttccggcttc
    19981 tccgggtaat cgggatggat caaccaaagg gcataaacaa agggcgtttt catccgctct
    20041 ttccattccg cacccaggtc gagaaactga tggcgccggc cggactgttg tcggaaaagg
    20101 atcgcctggt cgccgatcaa gagctgaccg cgctcttcgc tgatttctcc ttccgggacg
    20161 aatcgaaccg ccacaccctg ctcgccgagc aaacagcgga gcaggttgat ggatgtctcc
    20221 gaggcggggt cgacaatgac ttcgcggagc gcttcgactg cgccgagatg cgcgagaatg
    20281 acgctgaaaa ccggaccatc cgacgcgagc gcaagcccgt cgacgatcga gtagatcgga
    20341 ttgcgcaggt attcaaagct ggagaccaac gcgacattca gctcgcctgc tgccagttgc
    20401 cggcagagcg ccgacggatg atcgaatcgg acggcaccgc cccaaccgtg aatcagcggc
    20461 agggcgttgc gataccggac gcaaccgaga cggagcgaag ccggaaccgg cacttcaagc
    20521 gcagaccaat tcgccttccg cacgcggtgc agcgctcgct gggaggtcgg atttcgccgc
    20581 cgggatatga ctgtagtaac tgtcgcgctg caccgggtcg cggccggctt cgcgaattac
    20641 gtggcgcaac atctcgacgg tttgctcctc cggtgtcgtc acacccgcca tgtggaagat
    20701 tttttcccgc atgatcgtgc cgtgcaggtc gtccactccg taagccaggg cgacctgggc
    20761 aagcggtaat cccatgctga cccaataggc ggtgagatga tcgatgttat cgagataaat
    20821 ccggctcacg gccaggttgc gcagttggtc gaaggcggtc gccggtttga tatgcgccag
    20881 aatggtagtc tgcgactcaa aggcaaaagg gatgaaaccg gtgaatccgt aagtctcatc
    20941 ctgcagttcg cgcaactgac gcaaatgatc gacccggtgc tcgtgcgact cgatgtggcc
    21001 gtaaagcatc gtgcaagtgc tacggccacc catctggtgc cagatgcggt ggacttcgag
    21061 ccattccgcg gcgctctctt tcccgcggca caattcgtcc cgcacgcccg ggtcgaaaat
    21121 ctcggcgcca ccgccggtca gcgaattgag ccctgcctcg cgcaaaacgc ggagggtttc
    21181 tgcgatcgag gtcctaaaga tgcgccgcgc caagtgccgg atttcgatcg ccgtaaacgc
    21241 tttcagatgc agctggggat caagtgctcg cagacgagtg agcaggtcga gataccattg
    21301 tttgtcgagc gaaggatgga ggccgccgac catgtgcact tccgtgatgc cggtgaggag
    21361 cgcttcctgc accgcgacga cgatctcgtc gatcgagttc tcgaaagcgt gcgggtcgcg
    21421 cttcttggcg gcgaaagcgc aaaactggca ggagagcaca cagatgttcg aatagttgat
    21481 gtaacgattg aggacgtagg tggcgacgtt gccgtttttc cgctcccgca cgacgctggc
    21541 catgatcccg agagcgttga gatctctgga ccggtagagt tgcagggcgt cctcatcggt
    21601 aatccgctcg ttacgctcga tcttgtcggc aatcggccgg agcgtcggtg ggatgagcga
    21661 aagtttcatg aaggatatcg cgttcggtgc ggggaaacgt gggcggctta cgatgcacag
    21721 gccagcggag cgacgcaact aaaagccacg aacagaaaaa caaagagggc gaacgtctcg
    21781 cgacggttcg cccccttcca accccacaac aaattcagtt ttagttatcg cgctcgtcgg
    21841 ccgtgctcat atagcgcatc ttggtcggtg ccggcacgac gccgggtttc gggtaatcgc
    21901 tcgcgatctg ccgattcgaa gtcggaacca accgcaccgc gcccttggca ttattccgcc
    21961 agccgtttgg atcggcatat tcttctccgc tgtgagtcag gccgctcacc tgatcctcca
    22021 gaccggtccc gtagcgctga gcgagaatcg tgggcgtgac aaagacgagc agattgcgct
    22081 tggtgcgcgc attcagtttt tcctggaaga agtagccgag aacggggatg tcgcccatga
    22141 tcgggacctt gttgtgggct ttggtcactt catcctgcaa caagccaccg atcgcgaggg
    22201 tatcgccgct cttgatcaag acgttcgagt ccaaggtgcg ggtatcgatg attgggctcg
    22261 ccaccgtcgc gcccgcgaaa acaaaggtag catcggccac cttgttgctg atctctggct
    22321 tcaccatcag ggtgatgaaa tcgtccttat tgaccgacgg cctaacgacg agggtgttgc
    22381 cgaatttttt gtcctgaaac ccgccgaaca cggccgtcgc ggtttgctcg ttgaagttca
    22441 gttgcgggac tggctggttg cgaatgattt cgattttggc ctgctggtta tcggccgtca
    22501 ccacccgtgg gttagccagg aactcggcat cggaatcctc attcaaagcg cgcagggtaa
    22561 gagacatttg cggcacagag agaatcgcaa attgttccgg aatcagattg ccgaaccagc
    22621 tgtgggacgt cttagcgagg tccgtcgtcg gtatgcccgt tgggtcgatc gtcgttgttt
    22681 gagttgtctg gccaatagcg ccgtatttca ccgtctgcgg agtggtcgag ctcccgacca
    22741 ctcccgccca gttaatcccg tagctctgct tcggattcgc ggtaacctcc acgaggcgcg
    22801 cctcgatcat gacctgcttc gtgggcttgt cgatttgcac gagcatcttg cggatgttgt
    22861 cgatgttgct gcgcgtctcg cgaaaaaaga cggtgttggt tcggtcatcc acttgcggcg
    22921 cttctttgct cgaaagctgt gccgcgagca gcggcgcgac atccttcgcc cgagcgtaac
    22981 tgaatataaa gttgtcgctt tccgtcggtt cggccgtgcg ctcggcgcca gtcttgatgt
    23041 agtaaacgtt gtcgatctgg tccatgaaca gacccttcgc cttgacgatg atcgccaccg
    23101 cctgcaacgc cgtcacgtct tcaagccgca tcgtcaccgt gccggtgacc gcttcactca
    23161 cgaccatgtt aatcttcgcc tgccgtgcga gaagccgcag cacctggccc acgtcgtcgc
    23221 cttgaaattc ccgcacaccg acaccgccac tttccgtaat cgaattggtc accgttggtc
    23281 cggtggccgt ctgcgaaccc gccgtggtcg ccggcattga cccggaggac tgcgccgctg
    23341 tctgggtcgc cgagcgggcc ggggggggcg ccgcctgcac aagcgtaatg ggagcaacgg
    23401 cgggaggcga cgcctgcgcg agcgcaacgg gagcaagttc cgagcttcgt gcgacgctcg
    23461 cggaaggaac aggctcggtc tgcttcgccg gttcggccgt tttcccgggc tcggccgaag
    23521 tcgtgtgcgc ctcttgagcc aaggagacgg acgcggcgat cagaagagcg gcgagcgcgg
    23581 aaatgagtcg tttggttgat gtgggcataa tggatttggc gaaggtaatt tggactgcgg
    23641 actgaggtaa aagcaggaac cagaccagtc ctcgactgac tttccaaagg agtctccgcg
    23701 tcatgcgccg tcgccgcgtt gacgttaggc aacagcaggc agataggcga caggacgcct
    23761 gagcaccgcc cggccccgga gacgatgggc aaactggact cgccgcgtcg cgtccccccg
    23821 gcgggacgcg cctcctccgc catgagttgt caggccgcgg ttgcgaaacc gagcgccggt
    23881 ttgctaaagt ccgccgcagg catttgccta acgagtcgga caattccgtc ctgccacttc
    23941 cggccaatct cgagcaaata cggatcgttc tcttccaggc gggtgcgcat cttcacttcg
    24001 agtgagtcgg tcttgtagca aaggatgtcg atcggcggac cgactgcgac gttgctcttc
    24061 atcgtcgcgt cgatcgagat gatgccgaac ttgagcgcgt ccatcagcgt gctctcgtag
    24121 ctgaagccgc ggtcgagaat cggcttgccg tacttcgatt caccaatctg caagaacgga
    24181 caatcccggc tcgcgtgaat gctgttgccc tgcggataaa tggagtgcac ctcgggcgcg
    24241 agaccggcga tctgtccgcc gacgatcagg ttgatgttga aatcgaaacc gtcggcctcg
    24301 agcgcggcgc gatcgcgctg ctccacttcg cgcaccttcg agccgacata acgaaccgtt
    24361 tcatacatcg tctggcgatt catcaggtgc ttccgggcgg gatgactctc gttgagatgc
    24421 agatcgtcat cgatcaaggc cagggtcgcc tgggtcagcg agagatttcc cgagctcatg
    24481 atgcagatga cccggtcgcc cggcttctcg aaaacgcgca tcttggtgcg caccgtgatg
    24541 ttgtccatcc ccgcgctcgt ccgggaatcg gagagaaaaa ctgtgccctt gcggcagagc
    24601 atgccaaggc aatacgtcat gattcgcccg gcttgctact cccgacgacc cggccttgga
    24661 cccggcgcat ctgcgcgtga tgggtgagcg gatagacccg accgtcgctg ccgcgaaaaa
    24721 atgcgctcaa catccagctc accagaccga tgaggatcgc gccccaaaag gcgctccaga
    24781 agccatcgac atgaaagccg tgcaccagat cgttcaccag gtaaagcagc agagcgttca
    24841 cgacgaaaat gaaaagtccg agggtgagaa taatcagcgg cgccgcaagg aggagcaaaa
    24901 tggggcgaag gaaagcgttg aggatgccaa ggagcagcgc agcgccgaga agagaagcga
    24961 cgttgtcata acgaatcccg ctgatgatcg aagccgcgac catgacggcc acagtcgtga
    25021 ttgcccatct gaaaacaaaa tgcctcatcc gaagtgtgac ggccgcgaga aactcggttt
    25081 ccgcttgcca gatcgcgctc ttgtgggtaa aggcttggcc gacctcaagc aaaagtaaat
    25141 ggtgaaaaat tcaaaaaaat caaaagaaac cgcaattccg gaaagtaaaa cgcccatacc
    25201 cagcccggct gccgggaacg gtgatcgcgc cgctcattcc gcggaaccgg tcggcgcaaa
    25261 acgcgcggcc gcgaaaagcg taaagccagc cagaaacaca aaatcgacca gcaagagaca
    25321 agccggagcc gtttcgacgc gcaagccccg agccggcgcc gaacgcaagg atcgagccgg
    25381 caaagtcacg atctccgagg agcaaattcg gctccgcgcc tacttcatcg ccgagcggcg
    25441 gatgcagcat ggcatcccgg gagactcggc gcacgactgg ctggaagcgc agcggcagtt
    25501 gcaaaaagaa gcgaagaaac gcgcctaagg aggcgctggc ctacgcgatg gcgcgcgctt
    25561 ccggaaagca ctctttcacc ttttccagga gccgattgat cgtaaagggc ttgggtagga
    25621 atccgtggac ccgcgcctct ttcagcagct cctgcgcacg ttcatcggaa ccctgccccg
    25681 aaacgagcaa taacggcggc actttgcgcg gcggtgaggc gttttcgtag accttcgaga
    25741 tgagcagaaa aagcagtgac ccgtcgatca gcggcatcga aaaatcgaag atcatcagat
    25801 cgtagtcctt cttcagcgcg agttcgaagc cgtactccgg cttcggcgac gtctccactt
    25861 cccagtgcag aaaattctgg agcgtgtcgc ggatgatgcg aaggatggca acgtcgtcat
    25921 ctacgatgag cacccgtttt gcgtctgcct tagccatctc gcaggcttgc gcaaaatcgc
    25981 gacccaattc aagctttagt tgcgtccctt gacgaaaagc atgcgcccga tgaactcgcc
    26041 cgaaaacgaa cttgccggcg ccctccgtga acggctcgcg ctgatcgccg acgaggcgag
    26101 ccggcgcgag ccggcgagcc acctcctgaa actgaaatca gtttcagaca agatcgccac
    26161 cctggagcga gcgctcgcgc caccggtcga tccgcaactc gcacacttcc tcgaacggtg
    26221 cagttacgac aaagctctcg cgctcctcga agcgcgactg tagaggcgga cgcggaaggg
    26281 acgcgctggc ggcagagact gagggacggg tttgtgggca acgcacccgc cgctacatct
    26341 ggaatccgcg ctggcttacc gtgaatacaa ctcgacgatc aactgctcgt tcaccatcgg
    26401 ctgcatctct tcccgcgacg gaatgtgcgc gacctttccg gtcaacgcat cacgatcgac
    26461 cgtcagccag tccggcactg gcgcaatttg cgtcagctcc atgttgcgct gcgcgagctg
    26521 ccgcgatttc ggcctgttct tgaccgtgat ttcatcgccc gctttgacgt tgaacgaaga
    26581 aatgttcacg ttccggccat tgacctgcac gtgcccgtgc gagaccaact ggcgcgcagc
    26641 gctgcgcgta ttggccaggc caagccgaaa aacgacgttg tccaaccgcg tctcgagaag
    26701 ctgcaagagc gtctcgccgg tcacgccacg acgccggagc gccgcttcga aaatccggcg
    26761 gaattgcttt tccatcaggc cgtattggaa gcgcagtttc tgtttttcgc ccaacgcgat
    26821 cgcgtagtcg gattgcttgc gccgggagcc tttcgggcca tgcatgcccg gcggataatt
    26881 cttccgctcg agcgacttgg agggtccgaa aaggggcacg ccataacgac gtgcgatttt
    26941 agtcttgggt ccggtatatc tggccattcg aaaagttgag agtgattgtt aagacttggg
    27001 acggatcaga cgcgacgctg tttgggcggt cggcaaccat tgtgcgggac cggcgtcaca
    27061 tccctgatta cggtaaggtc gaggccgatc gcttgcaggg cgcggacggc cgactcacga
    27121 ccggcgccgg ggcccctgac gagcacttcg acttctttca gaccgtgacc catcgcctgg
    27181 cgggaagcgt cttgcgcgac catctgggcg gcgtaggcgg tgctcttacg cgaacctttg
    27241 aaccccacct tgccggcgct cgaccagccg atcacgttgc cttgcaggtc ggtgatcgtg
    27301 acgatcgtgt tgttaaaagt ggaaaggaca tgcgcgaaac cggagtagac gtttttgctc
    27361 cccttcgctt tgatgatttt cggcttctcg acgtcgttcg ggtcgagcga aagattttct
    27421 tttggcgcct ccgctgctgg cgcgtccgct tttttggccg atttcttcgc ggccttgggt
    27481 ttcgcttccg tcttgccggg cgctggagca acctccgccg tcaccgaagc atcggagggt
    27541 tggtcagatg gatttgcact ggtcgcctgt tcctcgggaa cgatcgcttt gggactggtc
    27601 ggttctgcct tcggcgcctc gggcgcaggc acttcctctg gagtttggtt ttcggaatct
    27661 tctgccatgt gtcagttcgt taggttaaac tttgccggtc ttcgcatcct tgttgcggat
    27721 gacgcccacg gtcttgcgcg gacctttacg ggtccgggcg ttggtcgagg tgcgctgccc
    27781 acgcaccggc aatccgcggc ggtgccggat gccgcgataa cagttaatcg cctgcaaacg
    27841 tttcaggtta ctctgcagtt cgcgacggag atcgccctcg atcggcagct tgttgctcgt
    27901 aatcatctgg atgatctcat tgatctgctg cggggtcaga tccttggccc gcaggttcgg
    27961 atcaatgttc gtctgctcga ggatgcgctt cgcggtgctc gggccgacgc cgtagatgta
    28021 ggtcagcgag gcttcgatgc gtttatcagc gggaatttcg actcctaaca atcggggcat
    28081 aacgatctcc tatccctggc gctgcttatg gcgcgggttc ttgttgcaga tcacgcgcac
    28141 cacattcttg cgcttcacga tcttgcacgc ttcacaaagt cttttaactg atggtcgcac
    28201 tttcatagtt cacgagacga aaaacgcgga gcctgcctcc gcgtcaccga tggcgaaaag
    28261 tgattcgccc tttcgacaaa tcgtaaggcg aaacttctag cataactttg tcgcctggca
    28321 agatgcgaat gaagtgcatc cgcatttttc cggagatgtg agcgaggacg ccatggccat
    28381 tgggcagttc cacccggaac atcgtgttcg gcaggagttc gaccacggtg ccttctacct
    28441 caattgcgtc tttgcgcgcc atgtcaggat ttcgggttcc tctttcgtaa tcaaaaccgt
    28501 gtgttcgaaa tgggccgaag gcaaaccatc cgacgtgcaa actgtccagc cgtcctcgag
    28561 cagccgcacg cccgatccgc cgagattaat catcggctcg atcgccaacg tcatgcccgg
    28621 cttgagacgc ggaccgctgc ctcgtttgcc gtaattgggg acctgcggct cttcgtgcaa
    28681 cttgcgcccc accccatggc cgacgaactc acggaccacg ctgaaatggt ggcgcatcgc
    28741 ctcatcctca atcgccgcgc aaaggtctcc gagccgttcg cctgaggcgg cgtgcccgat
    28801 ggcccgttgc aacgcgcttt ccgtaaccgc gagcaatttc tcgatgcgct cgtcgatcat
    28861 ccccaccgga atcgtcgccg cggtgtcgcc gacccagccg tcctgcacca ccccgatatc
    28921 gagcttcacg atgtcgccat actggatgcg gcgctggctg ccgatgccat gcacaatctc
    28981 gtcgttgagc gaaatacaga tattgcccgg aaagacccga tgaccgagtc gataaccaag
    29041 gaacgcgctc cgcacgccgg cgtcctgcat caaatccgcc gcggcctgat cgatttcctt
    29101 ggtggaaatt ccgggacgaa tcaactcgcc gacgcgctcg aggacatcgc tcgccgttcg
    29161 gcacgccagc cgcattttgt cgatctcccg ggcggacttg atcggaatca tggcacgcgt
    29221 tcctcgatca gggccacgat atcggcaaag accgattcgc ggtcgcgatt cccgtcaatc
    29281 cggtggaggg catcaccttt ttcgtaaaag gagagcaacg gttcggtctt cgccttgaac
    29341 tctacgaggc gcgtttgcag gaccgcggca tcgtcgtcgt cgcgccgaat gagcggccca
    29401 tcacagtagg gacagaccgg ccggtccgcg aaatgtgcac ttgttaggct agtggtgaag
    29461 ccacaccggc gacactgcaa ccggctggcg atccgctcgc ggactgtctg ctcggaaacc
    29521 tccagccaga gggcgaggtc gagcgccgcc ttgcgctttt gcagaatctc gttcaatcgc
    29581 tccgcctgct tcacggtgcg cggaaagcca tcgaagacaa acccgtcccg gccgtggaga
    29641 ttaagccaat cttcgatcaa atgaacgatg atgtcatcgg gaaccaggcc accctgtttc
    29701 gaaatctcat ccgcttccaa cccgagcggc gtctccagat cgcgctcgcg ccgcaggatg
    29761 gttcccggcg aggtcactgc gatggcgaag tgtcgtgtga tcatctccgc ctgcgttcct
    29821 tttccggagc cgggcgcacc gaggagcaca attcgccttt tcacctagcg cccgtaaaga
    29881 aaaatggcca cgcctccgat gaccagcacg gcgatcccga cgtagagcca catcagcgtc
    29941 ccctgctggg ccgcctcacc acgaccgtag gctgagcggt tgtcaaaagc acggccacga
    30001 attcggccct tgcgcaggaa tccatcatag tggcgctgga tcaggtgcgt ctccacctgc
    30061 cgcatggtat cgagaatcac gccgacgata atcagcagac tggtgccgcc aaaaaattgc
    30121 gcggtgatga ccggcacgtg caatccctga ctgagcaggc tgggcaggat ggcgatcatg
    30181 gtgaggaaaa tcgcgcccgc aaaagtcagt cgcgtcatcg tgaaatcgag gaaatccgcg
    30241 gtcggttttc ccggtcgcac gcccgggata tagccaccgt attttttcag gtcatccgcg
    30301 atctgggcgg gctggaactg cgtcgccacc cagaagtagc tgaagaagaa aatcattgcc
    30361 gcgagaacga cataatgcag ccagccagtc gaaagcatat tggcgatttc ctgggcagtg
    30421 cggctgttct tgaaggccag accgacgatc gttgtcggga agagaagcag cgcccaggcg
    30481 aagatgattg gcatgacgcc cgcgtagttc accttcagcg gcatgtattg cgtttgccca
    30541 ccgtacatct tccggccgac gacgcgcttg gcgtactgca ccgtgatctt gcgcacgcct
    30601 tgcgtcacgg cgatgaccgc cgcgatgacg ataaagagaa acgccaccat caaaacgagg
    30661 atcgccgggt tgacctgact ggtgcccgtc tccgcggaag ggacgaaggt cttccaggcc
    30721 tgcgccagtg cggccggcag gcgcgccacg ataccgatcg tgatgatcag cgagatgccg
    30781 ttgcctatcc ctcgatcggt gatttgatcg cccagccaca tcaggaaaag cgtgcccgcg
    30841 gtcagggtaa gaacagtaat gatgcggaac gaccagccga ggttatcaac cagcggaatc
    30901 cccagcctcg cggtcgtctc ggtgatgccg ggcagcatcg tgtgatacga ttcgggatgc
    30961 tgaaaggaga cggcgagcag gtaaccctga aagatgcaaa gcacgaccgt cgcataacgg
    31021 gtgtattgca tgatcttttg tcggccgcca tcttcgcgcg acaaacggct gagcgaggga
    31081 atcaccgcgg tcagcaattg cagcatgatc gacgcgctga tgtagggcat gataccgagc
    31141 gagaaaaccg cgcagttctc gagcgcgccg ccgctgaaaa gattgaagag ggcagcgacc
    31201 ccgccgcccg cggtctggga agaagtgcgg aaccaatcct gcaggaccgc cggattgacg
    31261 ccgggcgtgg tcacagccgc acccagccta acgatgacga tgaccgcgag ggtgaacaga
    31321 atccgccggc gcaactcggg gatcttgacg ctgtttaaaa aagccgaaac catcagctaa
    31381 gttcgcttca gacgttctgc ctattcagcc gtcttctttt tcttcgccgc gggtttcttc
    31441 ggcttcgcct tggctttcgc cgtcgcggaa gcgggcgttt ctcccttcgc tgccggcggc
    31501 tcgaaccgat ttccgctggt cagcgacggg cgtttccgga gcgtcacggt tccgccggct
    31561 ttctcgatct tttcgcgcgc tccttcgcta aaccgatccg cctcgatcgt gagcccgtgt
    31621 ttcaattcgc cccgacccag aattttcaag ccgacgacca ggccgcgaat gtgtttcgat
    31681 tcgcgcagca tctgctcgtt gatcaccgtg ccggccttga actggttcag gtcgtcgaga
    31741 ttgatcagag cgtaacgctt tttgaaagcc gcgttgttaa acccgcgctt cggcaaacgg
    31801 cggatgagcg gcatctgacc gccctcgaat ccgagccgca gactgccgcc ggaacgcgct
    31861 ttctggccct tgtggccctt gccgctcgtc ttgccatggc cggagctttc gccgcagccg
    31921 agccgcttga cgcgatgtct cgatcccggg cgcggtttca tgttgtgcaa ccgaatcatg
    31981 actgttgcct cgtctcgtcc acgttgttgc tgcgcaggcc gcgggtcttc aaaatttcat
    32041 cgcgccggcg cagggaacgg agcgcttcga tcgtcgcctt cacgacgttg gcgtgattgc
    32101 tcgagcctaa cgacttggca agcacgtcgc ggatgccgac cgcttccacc accgcgcgga
    32161 caccgccacc ggcgatcaca cccgtgccgg gcgaagccgg tcgcaaaagg acacgcccgc
    32221 cgccaaattc tccgatcgct tcgtgcggaa tcgtgttttc gtgaagtgac attttttcca
    32281 tcgacttgcg ggccgcttcc gaggctttgc ggatcgcttc cgcgacttca ttcgccttgc
    32341 cgaagccaca gccgactttg ccgtcgtgat cgcccgctac aatgagcgcg ctgaaactga
    32401 agcggcggcc gccctttacg accttggcgc agcggttgat gaacacgact ttttcggtcg
    32461 tatcgcctct tgccggaggc gtccggtccg tggtgcgtga gtcgcttcgt ctgccttggt
    32521 tgcctgcgta tgccatgaat aattaaagtt tgagaccacc ctcgcgcgcc gcgtcggcga
    32581 gcgctttcac tttgccgtgg taaagaaagc cgccacgatc aaaaaccgcc ttgtcgatct
    32641 tcttcgcgag caagcgctcg gcgatcaact tcccgacttt ctccgccgtc gcccgattgg
    32701 ccgcgtgttt tccgttgccg cccagttcct tttccgtcgt cccggccgag accagggtcc
    32761 gacccgtttc gtcgtcgacc acctgcgcgt aaacatgttt cccggaaaaa taaacacaga
    32821 gacgcggacg ttccgccgtg ccggaaacct tgcggcgaat gcgatcgtga atgcgcccgc
    32881 gaatgacttt gcgattgatc gtagccataa ctattggacg gttttgcctt ccttccggcg
    32941 aatctgctcg cctgcataac gaacgccctt gcccttgtaa ggttccggcg gatagaaacg
    33001 ccgaatgtcc gccgcgacct ggccgaccgt ttttttatcg gcgccgtgga tcgcgatctt
    33061 cgtgttatcc gtcaccgtga ttttgatatc cgtcggaatc gcgaagagta ccggatgcga
    33121 aaagccgaga ctgaggttga gattttttcc ctgcaccgcg gccttgaagc cgaccccctc
    33181 gatctcgagc tgcttggtga aaccttcgct cacgccgacg accatgttat ggatcagcgc
    33241 ccgcgacaat ccatgcagcg ccttgacgct gcgcgtttcc gcatcgcgga ccagcgagac
    33301 ctgattatcg gccacattgg cctggattcc cttcggcaac gtccagtcca acttcccctt
    33361 cgggccttcg accgcgactt tgctgccggc gacgttgact ttgactttcg cgggaatctc
    33421 gaccgcttta tttccaatcc gtgacatagc tctccttcgt taccagacgt aagccagcaa
    33481 ctcgcctccc accttttgtt tcttggcctc gcgtcccgac atgatgccgc gcgatgtcga
    33541 aaggatcgat gtccccatcc cgccaagcac ccgcgggatc tcatcggcgg cgacgtaacg
    33601 gcgcaagccc ggccggctga cgcgcttcag gccggtgatc gcgctggtgc gattcgcaat
    33661 tttattcgtg atcttgatcc gcggatgcgc ggcggtggta tcgagcgcgt agtcgctgat
    33721 gtagccctcc tctttcaaga tgcgaacgac ttcgctcttg atcttcgagt aagggacaaa
    33781 catctccagc ttctgcgcgt ggttgctgtt gcgcacgcgc gccagaaggt cggcaatcgg
    33841 atcggtgaca gtgctcatgg aagttaggcg gcttgcgcgg cgggcctttt cgccaggtcg
    33901 ctgaagggca ttcccatttc gctcaggagc gatttggcct cttcgttggt gcgcgcagta
    33961 gtcacaatcg taacatcaaa accgatgttg cgcttgattt tgtccagctc gacttcgggg
    34021 aagatcgact gatcggaaac gccgagcgtg tagttgccat ggccgtcgaa acccttcggc
    34081 gaaacaccgc gaaaatcgcg aatccgcggc agcgccgcct tgatcaggcg ctccatgaat
    34141 tcatacatgt gctctcctcg gagcgtgacc ttggcgccga tcgcctgctc tttgcgcagc
    34201 ttgaaattcg agatgctttt cttggcccgg gtctccgccg gtttctggcc ggtaatgagg
    34261 gccagctccg ctttcgcttc ttccagtgcc tgcttcacat ccgattgcga gccgaccgaa
    34321 gtgttgatca ccactttctc caacctcgga atctggtgtt cgttcttgta gccatggacc
    34381 tcgcgcagcg ccggcatgac gcgctctttg tattcgcgat agaattcgtt tttcattgct
    34441 ccgcccaaat tactttgtct tcttctcctt cgtcgccttt gccttgctct ttttcttcgt
    34501 ctccttctcg ccgccctcga ccagctttac gttcgagatg tggatcggac cttcgcgctc
    34561 cgcgatcgag cccgagggat tgtcctgcga tttgcgcagg tgcttcttaa tcatccgcac
    34621 gccctcgatc agcacgcgat ccttgcgcga aatcacctgt aggatcttgc cggtggaacc
    34681 ccggaagttg cccgagatga cctcgacctg atcgcccttt ttcacatggt gcttcagccg
    34741 gttcataaaa cttcgggagc gagcgaaacg atcttcatga aattgcgctc gcgcagctcg
    34801 cgcgcgaccg gtccgaaaat gcgggtgccg cgcgggttca aatctttgtc gatgataacg
    34861 atcgcgttgt tgtcaaaacg gaggagcgaa ccatcatcgc gccggatcgg ctgcctggtc
    34921 cggaccaaaa ccgcgcggac cacctcgccc tttttcacgg tgccagtcgg gctcgcttcc
    34981 ttgacgttcg ccgagatgac gtcgccaatg ccggcggatt gcgcgctgcc tttgccgatc
    35041 acgccgatca tcgtcgccat ccgcgcaccg ctgttgtcgg ccacgtccag tcttgatctc
    35101 aggtaaacca taatattctc ccctactctt tacttctgga cgacttcgac gagacgccac
    35161 cgcttcaact tgctcagcgg ccgcgtttcc atgatgcgca cggtgtcgcc agacttcgct
    35221 ttgttctcct cgtcgtgcgc ataaaatttc ttctgctgct tgacgatctt gccgaacttc
    35281 gcgtgcggca cccgcgtcac ggtgcggacg atgatggtct tgttcatgcc gctcgaaatc
    35341 acttcaccca cccgcgtctt gcgtgtgc
//
LOCUS       DATMNS010000013         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4673132, whole genome shotgun sequence.
ACCESSION   DATMNS010000013 DATMNS010000000
VERSION     DATMNS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4673132"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..312
                     /locus_tag="VNW28_00545"
     CDS             106..312
                     /locus_tag="VNW28_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08435.1"
                     /translation="MTRSHDEEKNVPAPKIAFDFRNPVAIARINQPFAEGREISESGP
                     IAQRAEARAQSIKGPVVRPPQRKA"
     gene            complement(319..1824)
                     /gene="lysS"
                     /locus_tag="VNW28_00550"
     CDS             complement(319..1824)
                     /gene="lysS"
                     /locus_tag="VNW28_00550"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HXA08436.1"
                     /translation="MEEENELIALRRQKMEMLRARGVAPFGRSFETSGSIAEVREKFA
                     EGATLRAAGRITAHRNMGKSHFLDLHDASGRIQIYVQAKELGPEAMATFDLLDLGDFI
                     GVEGSCFLTKTGEQTLKVQNFEVLAKTLRPLPEKWHGLTDVEARYRQRYLDLVTNERS
                     REVFAKRIAILRGTREFMQSRGFLEVETPMLQTVAGGAAAEPFRTHHKALGLDLYLRI
                     APELYLKRLLVGGFNKVFEVNRNFRNEGISRRHNPEFTMLEAYWAYANFEKVAELVEE
                     LICHLAQTVVEGRAPSRPSVDSGTGQSPSLQVEHRDAEGNVTRTLNLARPWRRARYRD
                     LVSAAAGADWFSLTNEQRSAKARALGVEVQPQMADFEVTQHVFEKLIEEKTFDPLFVT
                     HCPKELVPLAKQNAEDNTLVDVFELIINGQEIAPGYTELNDPLVQRQRLLEQAGEETQ
                     KLDEDFLLALEHGMPPAGGFGLGIDRLTMLLTGAESIRDVILFPLLRPKRE"
     gene            1997..>2285
                     /locus_tag="VNW28_00555"
     CDS             1997..>2285
                     /locus_tag="VNW28_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08437.1"
                     /translation="MLSSKTIRATICRALIFLFITLLAPAARADDSKSIRNFAEAIVQ
                     LAPDVDPAEAQLVSVTSHQTARRLAKEWRVVPPADFQNFLIHIGRRKYGYCF"
BASE COUNT          422 a          739 c          679 g          445 t
ORIGIN      
        1 cgtcgggcat ttcagccacc gatttccaaa ggtggcgcgc taaagctgtt cgatctgaca
       61 acctcgcgcc cggagcagcg caagtagtcc gttcggtcct cccatatgac ccgctcccac
      121 gacgaggaaa aaaacgtgcc cgctccgaag atcgccttcg attttcggaa tccagttgcg
      181 attgcgcgca tcaatcaacc gttcgcggaa ggccgggaaa tcagcgaaag cggaccgatc
      241 gcacaacgag ccgaagcgcg cgctcaaagc atcaaaggtc cggtcgtgcg gccgccgcag
      301 agaaaagcct gaacgaatct actcgcgctt cggccggagg agcgggaaga ggatcacgtc
      361 gcggatcgac tccgcgccgg tcaggagcat cgttaggcga tcgatcccga ggccgaaccc
      421 gccggccgga ggcatgccgt gctcgagcgc gagcaggaaa tcctcgtcca gcttttgcgt
      481 ctcctcgccg gcctgctcga ggagacgctg gcgctgcacg agcggatcgt tcagctcggt
      541 gtagccgggc gcgatttcct ggccgttgat gatcagttcg aaaacgtcga cgagcgtgtt
      601 gtcctccgcg ttctgcttgg cgagcgggac caattccttc gggcaatgcg tgacaaagag
      661 cggatcgaag gttttttcct cgatcagttt ttcgaagacg tgctgggtga cttcgaaatc
      721 cgccatctgc ggctgcacct cgacgccgag ggcgcgcgct ttcgcggaac gttgttcatt
      781 cgttaggcta aaccagtcgg ccccggccgc ggcggaaacg agatcgcgat agcgcgcgcg
      841 gcgccagggc cgggcgaggt tcagcgtgcg ggtgacgttg ccctccgcgt cgcggtgttc
      901 tacctggagg gatgggctct gtcccgtccc cgaatcgaca ctcggacggg acggcgcccg
      961 tccctccacg acggtctgcg cgaggtggca gatcagctct tccaccagtt ccgcgacctt
     1021 ctcgaaattg gcgtaggccc agtaggcctc gagcatggtg aactccgggt tgtgccggcg
     1081 cgaaatgcct tcgttgcgga aattgcggtt cacttcgaag accttgttga acccgccgac
     1141 gagcagccgc ttcaggtaaa gctccggcgc gatgcgcaga taaagatcga gcccgagcgc
     1201 cttgtgatgg gtgcgaaacg gttccgccgc cgcgccgccc gcgacggttt gcagcatcgg
     1261 cgtctccact tccagaaagc cacgcgattg catgaactcg cgtgtcccac gcagaatcgc
     1321 aatccgcttc gcgaacactt cgcgcgagcg ctcgttcgtc accagatcga ggtaacgctg
     1381 ccggtaacgc gcttcgacat cggtcaatcc gtgccatttc tcgggcaacg gccgcagggt
     1441 tttcgcgagc acttcgaagt tctgtacttt caaggtctgc tcaccggtct tggtgaggaa
     1501 acaacttccc tccacgccga taaaatcgcc gagatcgagc aggtcgaagg tcgccatcgc
     1561 ctccggccca agttccttcg cctgcacata aatctggatg cgccccgacg cgtcgtgcag
     1621 gtcgagaaag tggctcttcc ccatgttgcg atgggccgtg atccgaccgg cggcgcggag
     1681 cgtcgcgccc tcggcgaact tttctcgcac ctcagcgatc gagccgctcg tctcaaagct
     1741 ccgcccaaac ggcgccaccc cgcgggcacg cagcatctcc attttctggc gccgcagcgc
     1801 gatcagctcg ttctcttcct ccatcaggaa tccgaatgta atcggattct tccgtctgcc
     1861 cagattttcc cgtggacagg ggaccgcacg gcggattgct cgcgtcttcc aagtgaccca
     1921 gccgccgcct acggcacggt cgatttggac ttgaacggaa aacgcctact gtcgtgaccg
     1981 ttcgattatg ccaccgatgc tctcttcgaa gacaatcagg gccacgatct gccgggcgct
     2041 cattttcctg ttcatcactc tgctcgcgcc ggcggcgcgc gcggacgatt cgaagtcgat
     2101 ccgcaatttt gcggaagcga tagtccagct cgcgcccgat gtcgacccgg cggaagcgca
     2161 gctcgtctcc gtcacctcgc atcagactgc gcgtcggctg gccaaggaat ggcgggtcgt
     2221 tccgccggca gacttccaaa atttcctcat ccacatcggc cggcgaaagt acggctactg
     2281 ctttc
//
LOCUS       DATMNS010000014         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2528195, whole genome shotgun sequence.
ACCESSION   DATMNS010000014 DATMNS010000000
VERSION     DATMNS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2528195"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            34..579
                     /gene="recO"
                     /locus_tag="VNW28_00560"
     CDS             34..579
                     /gene="recO"
                     /locus_tag="VNW28_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXA08438.1"
                     /translation="METSKAILLRKRKLSDTSLIVSWYAESLGRIDTAAKGARRPKSV
                     FAGKLDLFFEAEIQIARSRRSSLHTLTEVVLRNPFGGIRANFLRTQTASYFVELIELC
                     TEPDHSAPELFHLLQRAFGFLDAQDASLRAVRHFETELARLAGVHDATVLKSDPAAAL
                     GALFGRLPRGRAALLKALAES"
     gene            627..1220
                     /locus_tag="VNW28_00565"
     CDS             627..1220
                     /locus_tag="VNW28_00565"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXA08439.1"
                     /translation="MKKHLPLLLLAAAICGPALPARADDTYKIDPVHSAISFKVRHFF
                     SYVDGCFTKFEGTINVDPDHPEKSSVTATIDAASVDTKNENRNEDLRGAHFFEVAKYP
                     TITFKSKSVKQAGADSGDVVGDLTMHGVTKEITLHVKFLGKGQGMGGKPISGWQVTTD
                     PIKRSDYGLTWSKAVEGTAVVGGEVTVSIDVEADKTP"
     gene            1342..2577
                     /gene="lysA"
                     /locus_tag="VNW28_00570"
     CDS             1342..2577
                     /gene="lysA"
                     /locus_tag="VNW28_00570"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXA08440.1"
                     /translation="MSFAYQDQELMCEGMAVAAIATRAGTPCYLYSAHQFRENFGAYR
                     NAFSGLDAAICFAVKACSNIAILKLLGAAGAGADTVSGGEIQRALLAGIPPEKIIFSG
                     VGKTKEEIALALESGIGQLNVESPEELEIVDAIAAARKVTARVSLRVNPDVDAGTHEK
                     ISTGRKSDKFGIPWPEVWETYQKARSLSHVEVEGVACHIGSQITDLAPFKAAFSKVTD
                     LARELQADGFPLRRIDLGGGLGIRYRDENALGVADYAAAVMQSVRPLGLRLFLEPGRS
                     IAASAGALISEVQFIKLAEGKRFAVLDAGMNDLARPAIYGAYHEIIPVREGDPAESYD
                     IVGPVCESSDIFGTNRRLPKLAAGDLVAILNAGAYGAAMSSNYNTRSLPPEILVEGDQ
                     CKVIRERQRFADIIALERY"
BASE COUNT          573 a          836 c          850 g          534 t
ORIGIN      
        1 agcagaccgt gtaggctcca tttctccccg cggatggaaa cctccaaggc aattctcctg
       61 cgcaagcgca agctgagcga caccagcctc atcgtcagct ggtatgcgga gtcgctgggg
      121 cggatcgata cggcggcgaa aggggcgcgc cggccgaaaa gcgtttttgc cggcaaactc
      181 gatctttttt tcgaggcgga aattcaaatc gcgcgcagcc gccgatccag cctgcacacg
      241 ctgaccgagg tcgtgctgcg gaatccgttt ggcggaattc gcgcgaattt ccttcgcacc
      301 cagacggctt cgtattttgt cgagttgatc gaactttgca ccgagcccga ccattccgcg
      361 ccggagttgt tccatttgtt gcagcgagct ttcggttttc tcgacgcgca ggacgccagc
      421 ctgcgcgcgg tgcgtcactt cgagacggag ctcgcccggc tcgccggggt gcatgacgcg
      481 acggtcctga aaagcgatcc ggccgcggcg ctcggcgcgc ttttcggccg cctgccccgg
      541 gggcgtgccg cgctgctcaa agcgctggcc gaaagttgag gaaacttctg gcgaccgttg
      601 cgcgaagcac taggttctcc gccgccatga aaaaacatct gcctctcctg ctcctcgccg
      661 ccgcgatttg cggcccggcc ctcccggcgc gtgccgacga cacctacaag atcgatcccg
      721 tccattccgc gatcagtttc aaagtgcggc atttctttag ttacgtggac ggctgtttca
      781 cgaaatttga gggcacgatc aacgtcgatc ccgatcaccc ggagaagtcc tccgtcaccg
      841 ccacgatcga cgccgcgagc gtcgatacga aaaacgaaaa tcggaatgag gatctgcggg
      901 gcgcgcattt ttttgaggtc gcgaaatacc caacgatcac cttcaagagc aaaagcgtga
      961 agcaagccgg cgcggacagc ggcgacgtgg tcggcgacct gacgatgcac ggggtgacaa
     1021 aagagatcac cttgcacgtc aaatttctcg gcaaagggca aggcatgggc ggcaagccga
     1081 tcagcggctg gcaggtcacg accgacccga tcaagcggag cgactacggc ctaacctgga
     1141 gcaaggcggt tgaaggcacc gcggtggtgg gtggagaggt cacggtctcg atcgacgtcg
     1201 aagcagacaa aacgccctaa ccaatttagc cgttcaggcc gctgagggcc cgcgcgattc
     1261 gccctgccgg ggcacgacgc gatcgatcgg gtgcgcggaa tcctcgaagc tggcgctaat
     1321 ggcgcggtga tggttccgcg aatgtcattt gcctatcaag accaggagct gatgtgcgag
     1381 ggaatggcgg tcgccgccat cgcgacgcgg gccggtacgc cctgctatct ctattccgcc
     1441 caccaattcc gggagaattt cggcgcctat cggaacgctt tttccggcct cgacgcggca
     1501 atttgttttg ccgtcaaggc atgctccaac attgccattc tgaaattgct gggcgcagcg
     1561 ggtgcgggcg cagacaccgt ttccggcggt gaaatccagc gcgccttgct ggcgggaatc
     1621 cctcccgaga aaatcatctt ttccggggtc ggaaaaacga aggaagagat cgcgctggcg
     1681 ctcgagtccg ggattggaca actgaatgtc gagtctccgg aggaactgga gatcgtcgat
     1741 gcgatcgccg ccgctcgaaa ggtcacagcg agggtctcgc tccgggtcaa cccggatgtc
     1801 gatgcgggca cgcacgagaa aatctccacg ggacgcaagt cagataagtt cggcattccc
     1861 tggcccgagg tgtgggagac ctatcaaaaa gcgcggagtt tgtcgcacgt cgaggtggag
     1921 ggagtggcct gtcacatcgg gagtcagatc actgacctcg ctccgttcaa agccgccttt
     1981 tccaaagtaa ccgacctggc ccgcgagctg caggccgacg gctttcctct gcgacgcatc
     2041 gatctcggcg gcggcctcgg catcagatat cgggacgaga atgctttggg cgtggccgat
     2101 tacgccgcgg cggtcatgca gtcggtgcgg ccgctcggtc tgcgactgtt cctcgaaccg
     2161 ggccgctcca ttgccgcttc ggccggagcg ctcatttccg aagtccagtt catcaaactc
     2221 gcggaaggca agcgcttcgc cgtgctcgac gcgggaatga acgatctagc gcggcccgcg
     2281 atttacggcg cctaccacga aatcattccg gtgcgcgagg gcgaccctgc cgaatcctac
     2341 gacattgtcg gaccggtctg tgagtcatcc gatatttttg gaaccaatcg aagacttcca
     2401 aaattggcag cgggcgattt ggtagcgatt ttgaatgccg gcgcttacgg cgcggcgatg
     2461 tcctcgaact acaacacgcg cagcttgccg ccggaaatcc tggtcgaggg cgaccaatgc
     2521 aaagtgatcc gcgaacgcca gcgtttcgcc gacatcatcg cgctcgagcg ttactagaaa
     2581 ttccgggcgg cgcctcgagt tgcaggcaag attttggaag gcaaaaagca attcttgaag
     2641 agccaatcgc ctcccgggcg gttagtctcc gctgcgagaa ggcaaaaatg aagaagaacg
     2701 aaattcataa acgatgatga atcgaatcct gaccttcatc cttgcgctcg acgttttgac
     2761 ctttcctctg gccgcgcagc catcaccgca gca
//
LOCUS       DATMNS010000015         2956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3590983, whole genome shotgun sequence.
ACCESSION   DATMNS010000015 DATMNS010000000
VERSION     DATMNS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3590983"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..846)
                     /locus_tag="VNW28_00575"
     CDS             complement(46..846)
                     /locus_tag="VNW28_00575"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXA08441.1"
                     /translation="MSASDAEATMAVFLDLENIALGAHDAQFPAFDVRKVLERLLLKG
                     HIVVKKAYCDFERYKAFKRGLHEAAFEMIEIPHVRQSGKNSADIRMVVDALDLCYTKS
                     HVDTFAIISGDSDFSPLVSKLRENDKTVIGVGVKQSTSDLFITNCDEFIYYDDLVRTE
                     PVRKRQAGSAAGLVKSRPEKNQGPPIDEALDLLTNTIDALRAERGEDYAVHGSRVKQA
                     IKRQHPGFNERAYGFRTFSDLLLEAQKRGLLKLESDKNAGGYFLHSEE"
     gene            913..1359
                     /locus_tag="VNW28_00580"
     CDS             913..1359
                     /locus_tag="VNW28_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08442.1"
                     /translation="MKRISKWLLPILGGALLSMAACHKLKPAAENATPSEEQVRATRA
                     AEVPKSIQQQWTCLNRIRQRDGLNSTIDRTLLDEQNQLGVVLFSTVTPDKVPALMRTV
                     MTEMAQEFPNDNVTLTVYASANPPRKMGTAHRDGQTGEVSYVPEKM"
     gene            complement(1460..2698)
                     /locus_tag="VNW28_00585"
     CDS             complement(1460..2698)
                     /locus_tag="VNW28_00585"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08443.1"
                     /translation="MNEPVYILGAGRTDFRRNLKKEGKALRDVIKDAGRAALNDAKID
                     PAEIKAAAVGNFNAGQFTKQLHLGAFIPEIDPKLHGIPTMHTEAACASGALSVLLGAQ
                     WIMGGFHDAVLVVGAEQQKTMSSRDGGDVLGAAADFDVEQPEYGDFMFPKLFGRIAQI
                     YIDKYGAGPDALAAVAYKNYAHARLNPLAQMRESTLTFSDAQQISEKNPSVAAPLRLT
                     DCSQITDGAAGLVLVSGKYLDRIGRDKSKFPRLLGFGHTTDYLALAKKDAPTFSTARK
                     AAANAFAMANLKPRDVHGAEVHDCFSITEIVAYEILGFAEPGKGAELAQSGATTLPQV
                     RGEHVSGKIDFEIPVNAGGGLIGDGHPVGATGVRQVVEANQQLTGQAGARQIKGAKKF
                     VTFNMGGSLTTSVAMIWGTD"
BASE COUNT          567 a          899 c          895 g          595 t
ORIGIN      
        1 gccaggccgc gaccccccag tcgtagcgcc gaccggctgc gctcgctatt cctccgaatg
       61 gagaaagtag ccgccggcgt ttttgtcgct ttcaagtttc agcaagccgc gcttttgcgc
      121 ttcgagaagc agatcgctga aagtgcgaaa accgtaggcc cgctcgttaa agcccgggtg
      181 ctgacgcttg atcgcctgct ttacgcgcga gccatggacc gcgtagtctt cgccgcgctc
      241 ggcgcgcaag gcgtcgatgg tgttggtcag gagatcgagc gcttcgtcga tcggcggtcc
      301 ctgattcttt tccggcctgc ttttcacgag cccggccgca gaaccggcct gacgtttccg
      361 caccggttcc gtgcgcacga ggtcgtcgta gtaaataaat tcgtcgcagt tggtgatgaa
      421 gaggtcggag gtcgattgct tcaccccgac gccgatcact gttttgtcgt tttcgcgcag
      481 cttgctcacg agcggcgaga aatcggagtc gccgctaatg atggcgaaag tatcgacgtg
      541 gcttttcgtg taacaaagat cgagcgcatc aaccaccatc cggatgtcgg cggaattttt
      601 gcccgactgc cggacgtgcg ggatttcgat catctcgaag gccgcttcgt gcaacccacg
      661 cttgaaagcc ttgtagcgct cgaaatcgca gtaggccttc ttcaccacaa tgtggccctt
      721 gaggaggagt cgctcgagga ctttgcgcac atcgaaggcc ggaaactgcg cgtcgtgcgc
      781 accgagcgcg atgttctcca aatcgagaaa gacggccatg gtggcctcgg cgtcggatgc
      841 gctcatggtt tagaggatgt aagcgcgatc gcagtcgcga aacagaaaaa tattcatagg
      901 gtcaagcttg ccatgaaaag aatctccaaa tggctgctcc cgattctggg cggcgccttg
      961 ctctcgatgg cggcgtgtca caaactgaag ccggcggcgg aaaacgccac tccttcagag
     1021 gagcaagtcc gggcgacgcg ggccgcggaa gtgcccaagt cgatccagca gcagtggacc
     1081 tgcctgaatc ggatccggca aagggacgga ctcaactcca ccatcgatcg cactctgctg
     1141 gacgagcaga atcagctcgg ggtggtgctt ttctcgacgg tgacaccaga caaagtgcct
     1201 gccttgatgc ggacggtgat gacagagatg gcgcaggaat ttcccaatga caatgtgacc
     1261 ctgaccgtct acgcctccgc caacccgccg cgaaaaatgg gaacggcgca ccgcgacggc
     1321 cagacgggtg aggtgagtta cgtccccgag aaaatgtaga cgcttcgctc tgcgaagcat
     1381 gcgatgtgat gtcgctcgca gctcggtcgt cgcggcggga atcaactctc gcgcctccca
     1441 ccgggaggcg gctacagaac taatccgtgc cccagatcat ggccaccgaa gtcgtcaggc
     1501 tgccgcccat gttgaaggtg acgaattttt tcgcgccttt gatctgacgc gcacctgcct
     1561 ggccggtcag ttgttgattg gcttcgacca cctggcgcac gccggtcgca ccgaccggat
     1621 gaccgtcacc gatcaacccg ccgccggcgt tgaccgggat ctcgaaatcg atcttgccgc
     1681 tgacgtgttc gccgcgcacc tggggcaaag ttgtcgcgcc gctctgcgcc agctccgcgc
     1741 ccttgcccgg ctcggcgaac ccgagaattt cgtaagcaac gatttcggta atcgaaaaac
     1801 aatcgtgcac ctccgcgccg tgcacgtcgc gcggcttcag gttcgccatc gcgaaggcgt
     1861 tggcggcggc cttgcgcgcc gtgctgaaag tcggcgcgtc cttcttcgcg agcgcgaggt
     1921 aatcggttgt gtgcccgaaa ccgagcagac gcgggaactt cgacttatcg cgcccgatgc
     1981 ggtcgagata cttgcccgaa accagcacga gccccgctgc tccatcggtg atctgcgagc
     2041 aatcggtcag gcgcaagggc gcggccacgc tcggattttt ttccgagatc tgctgggcgt
     2101 cgctgaacgt gagagttgat tcgcgcatct gggcgagcgg gttaagccgc gcgtgcgcgt
     2161 aatttttgta agcgaccgcg gcgagagcgt ccggaccggc accgtattta tcgatgtaga
     2221 tctgcgcgat gcgtccgaac aacttcggga acatgaagtc gccatactcc ggctgctcga
     2281 cgtcgaaatc cgccgccgcc ccgagaacgt cgccgccgtc gcgcgatgac atcgtcttct
     2341 gctgttccgc gccgacgacg aggaccgcgt cgtgaaatcc acccatgatc cattgcgcgc
     2401 cgagcaggac cgaaagcgcg ccggaagcgc aggccgcttc ggtgtgcatc gtcgggatgc
     2461 cgtgcagttt cgggtcaatc tccggaatga acgcgccgag atggagctgc ttcgtaaact
     2521 gtccggcgtt gaaatttccg accgccgcgg ccttgatctc cgccggatcg atcttcgcgt
     2581 cattaagggc cgcgcggccg gcatccttga tcacgtcacg cagcgcctta ccctcctttt
     2641 tgaggttgcg cctaaagtcg gttcggcccg cgccgaggat gtaaactggt tcgttcatgg
     2701 ctgcctgaaa cctagactac gccagctgcg ctgtcatccc caccagtctc gccggatcgg
     2761 cggcgttgcc tttgcgaaaa agccggaaga ggcgcggtcc cacgccgagc gcattagcaa
     2821 cgttacgttt cttaagtcgt taatctgcga gtgggccctc ggttcgagaa ctaggggttg
     2881 ccgtcgaaac tgctaccaag tcagtcgctc gattcctcga acgtgatcaa agtgcgcgtt
     2941 acggctgata atcggc
//
LOCUS       DATMNS010000016         8089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_381872, whole genome shotgun sequence.
ACCESSION   DATMNS010000016 DATMNS010000000
VERSION     DATMNS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8089
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381872"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VNW28_00590"
     CDS             complement(<1..324)
                     /locus_tag="VNW28_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HXA08444.1"
                     /translation="MKKILVAGIGNIFFGDDAFGCEVANQLARRALPEEVCVTDFGIR
                     SYDLAYAMMDNYDAIILVDASPQGSAPGTIYLIEPDQKKLDELPDEAVNAHSMNPVRV
                     LQLVRS"
     gene            complement(321..1667)
                     /locus_tag="VNW28_00595"
     CDS             complement(321..1667)
                     /locus_tag="VNW28_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08445.1"
                     /translation="MNAALVEKVVNAVLYEGYILYPYRASSQKNRERFTFGRVYPEDY
                     SVAQEGAEPSLMQTECLVRNESKDTVLKISVRFLHPMAREVGLLSVPLAELPAGEPPA
                     FEVVPDLQVGEQLYQTWQEVVEQKVELPPLALRAAAQISHPFDFPRVRTVEPIRAADG
                     KIIALLVRRQEGVCGVIEVGTQPIDDAVFKISVRILNLTPVPPDELEKQSAIIMRTFT
                     STHTILHAEGGECLSLTDPPAACAEAASACKNIGTWPVLVGDDARGERDAMLSSPIIL
                     YDYPKIAPESPGDLFDGLEIDEILTLRVMAMSDAEKIEMRQVDEQARKILERTENMSS
                     DHLLKMHGVMRGVRASNEDFFNPGTRLETATVGSVQVKAGDRVRIRPKKRADVMDMAL
                     DGKIAVIEAVEQDVEGGVQLALVLDDDPGRDLGLLRQPGHRFFYATDEVEPVPESR"
     gene            complement(1664..2365)
                     /locus_tag="VNW28_00600"
     CDS             complement(1664..2365)
                     /locus_tag="VNW28_00600"
                     /inference="COORDINATES: protein motif:HMM:NF039986.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HXA08446.1"
                     /translation="MPDLDFKIVGVEAADYGIAPLLHFKLEITNAPEAETIQSVLLQA
                     QIQIQSTQRAYQPAEKEKLGELFGTPDRWGQTLRARLWTHASANVRQFTGKTEAVLSV
                     PCTYDLNVSATKYFYALEGGEVPLLFLFSGTIFYEGPDERLQIQQVSWNKECAYRMPI
                     SVWQEMMDHHYPNTAWLALERGLFERLYAFRRRESLANWEQTIERLLPPPNESETTET
                     EAASGDQAGAPLLRP"
     gene            complement(2365..2982)
                     /locus_tag="VNW28_00605"
     CDS             complement(2365..2982)
                     /locus_tag="VNW28_00605"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HXA08447.1"
                     /translation="MRSSSFATLRRFASAQRTPIAAPRAELEHCELCSVPLGPTHRHL
                     LEMATRRIVCSCDPCALRFENVVGGKFKLIPRDARALPDFQMSDAEWEGLSLPISLAF
                     FFHDTPNEKMAAYYPSPAGATESLLPLTAWEALVVANPVLRDMQADVETLLVNRVEGA
                     REYFLAPIDLCFELVGLIRIHWRGFSGGEDVWREIDGFFARLKGN"
     gene            complement(3065..3253)
                     /locus_tag="VNW28_00610"
     CDS             complement(3065..3253)
                     /locus_tag="VNW28_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08448.1"
                     /translation="MSKPPLAGHIPGTVRGEELALDNKEAGRGAGGKSYTTARDATGI
                     NPEARAPIHPDMPNLQPS"
     gene            complement(3250..3861)
                     /locus_tag="VNW28_00615"
     CDS             complement(3250..3861)
                     /locus_tag="VNW28_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HXA08449.1"
                     /translation="MQETSKQEFQKQLGRAEELIAALEGTPDPAAREQVRELIQILLD
                     LHGTGLERVLSVVFDSGTEGAAMIDELARDHLVSGLLLLHGLHPLDLETRVRNAIEEV
                     KPRLGLHGGSVELLAVTPEGRVQLKLQGNCHECPSSRLTLRFSIEEALNAAAPDLTGL
                     DVEGLPEEHPKPAKNGTTPIPKFTDCPLPTNGSGPRAGAGGNI"
     gene            4085..4531
                     /locus_tag="VNW28_00620"
     CDS             4085..4531
                     /locus_tag="VNW28_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08450.1"
                     /translation="MPKASLTNDPFFVAVVAAIERKIAEGDRIARANSITLSDAQILA
                     LLTRAAGSACGKAAKTSPPRLPRDQILTALAEQLALLRASIFEKKIDRDGAETETPLS
                     AGHWIAALKVVQKSIRRGTGHLRGSRSFLDSLSIFMARAASAGSKR"
     gene            complement(4688..5827)
                     /locus_tag="VNW28_00625"
     CDS             complement(4688..5827)
                     /locus_tag="VNW28_00625"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXA08451.1"
                     /translation="MNRLEESKRSRYMEWAKTCSTARFNLATSGLTNVLLSEFPLRLE
                     ELEITGGGYGYAPLLERIARHTGAPVECIVTAEGTSMANHLAMATLLAPGDEVVIEQP
                     TYGLLLDVLHYLGARVKRIARTFENNFDLALPAVGQAITPATRLVVLTNLHNPSGALI
                     RSETLRAIGEMAQRVGAHVLVDEVYLEMLFPGTAGTSGSSSLRDSPRRVQDHASFSLG
                     ENFVVTSSLTKAYGLSGLRCGWILAAPELARRMWLLNDLFAATAAHSAERMSVVAFDQ
                     LARFRARTAALLTPNRALLDSFLDSRADLECFRPPAGTVVFPRLPRGEPELFFKLLRE
                     KYETSVVPGAFFESPRHFRIGIGGETENLRAGLERLGAALDEFLA"
     gene            complement(6032..7396)
                     /locus_tag="VNW28_00630"
     CDS             complement(6032..7396)
                     /locus_tag="VNW28_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXA08452.1"
                     /translation="MTSGAGHASTPNEDGKLARQLGLFGTTTAVIGGIVGAGIFINPY
                     LVAQRVHTAPLILGAWIAGGLIALAGGFIYAELAARLPVVGGQYAYLREALHPAVAFL
                     YGWVLLLVIQTGGMAAVAVTFARYFLELTQWPLPDAFVAAAALGLLTAINCLGVRAGS
                     RLQATLTLTAIGAIALLEAVSFWRGATSRIAWRPALDQPASLNLVVAFGAAMTPVIFA
                     YGGWQTTSFLAGEVRDARKTLPRGLILGVLGVIAVYLSVNFVYLRELGASGLAATSTP
                     ASSVMRGLLGARGGQLIAAGIAISAFGFLAQSMLTAPRVYFAMARDGVFFRSVARVHP
                     RTRVPMVAIMLQGGWAIVIALAGTYAQVVNYVIAMDCIFFGLTAVCLFVLRRRAPAPA
                     SYRVPGHPWTTLLFIAAQWMIVVSTFAHDPKRACIGLGIALAGLPIYFLWRRGDKSVD
                     LSYR"
     gene            complement(7530..>8089)
                     /locus_tag="VNW28_00635"
     CDS             complement(7530..>8089)
                     /locus_tag="VNW28_00635"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXA08453.1"
                     /translation="RLRAEKVAAVYASPLGRTVETASIIAQPHGLPVVRRDGLREISH
                     GCWEQMTRREVEQKYPEEAAAWNEDPYTFAPAGGESGLAVTARALPVLVEIVRAHPGE
                     CAIIVSHKATIRLLLSSLLGFDPRRYRDNLDQSPAALNIVDFKDAAHARLTLFNSTSH
                     YSTTEYSMPSVPTARLSKWWDETGK"
BASE COUNT         1572 a         2585 c         2424 g         1508 t
ORIGIN      
        1 cgagcggacc agttgcagaa cgcgaaccgg attcatgcta tgagcattta ccgcctcgtc
       61 gggtaactcg tccagttttt tttgatcggg ttcgatcaaa taaatggtgc ccggcgcgct
      121 gccttgtgga ctggcatcca ccaggatgat tgcatcatag ttatccatca tggcgtaggc
      181 taaatcgtaa ctgcgtatcc caaagtcagt tacacaaact tcctcgggga gcgcccggcg
      241 cgctagctgg tttgcgactt cacaaccgaa ggcgtcgtcg ccgaaaaaaa tatttcctat
      301 tccggccaca agaatctttt tcatctgctc tccggcaccg gttccacttc atctgtggcg
      361 tagaaaaaac ggtgcccggg ctgccggagc aggccgagat cgcgccccgg atcgtcgtcc
      421 agcacgaggg ccagttgcac accgccttcg acgtcttgct cgaccgcttc gatcaccgcg
      481 atttttccat cgagcgccat atccatcacg tccgcgcgtt tctttggacg aatccgcacg
      541 cgatcgccgg ctttgacctg cacgctgccg accgtggcag tttccaggcg cgtgcccggg
      601 ttgaaaaaat cttcgttcga cgcacgcacg ccgcgcatca cgccgtgcat tttgaggaga
      661 tgatcgctcg acatgttctc cgtgcgttcc aggatttttc tcgcttgttc gtccacctgt
      721 cgcatctcga ttttttccgc atcgctcatc gccatcaccc gcaacgtgag gatctcgtcg
      781 atctcgagac cgtcgaaaag gtcgcccgga ctctccggtg cgatctttgg gtaatcgtat
      841 aaaatgatcg gcgacgaaag catcgcgtcg cgctcgcccc gcgcatcgtc gccgacgagc
      901 accggccagg tgccgatgtt tttgcaggcc gaggcggctt ccgcgcaggc ggcgggggga
      961 tcggtcaggg aaaggcattc gccgccctcg gcgtgcagaa tggtgtgggt ggaagtgaag
     1021 gtgcgcatga tgatcgcgct ttgcttttcc agttcgtcag gcgggaccgg ggtgagattc
     1081 agaatgcgga ccgagatttt gaagaccgcg tcgtcgatcg gttgggtccc gacttcaatc
     1141 actccgcaga cgccctcctg gcgccgcacg aggagagcga tgatttttcc atccgcagcg
     1201 cgaatcggct cgaccgtgcg gacccttgga aaatcgaatg gatgcgaaat ttgcgccgcg
     1261 gcccgcagcg cgagcggcgg cagctcgacc ttttgttcca cgacttcctg ccacgtttga
     1321 taaagttgtt ctccgacctg caaatcgggc acgacttcaa acgctggtgg ttcaccggcg
     1381 ggcaactccg cgagcgggac ggaaagcagg ccgacctcgc gcgccatcgg atgaagaaag
     1441 cgcacgctga tcttgagcac cgtgtctttc gactcgttgc ggaccagaca ttcggtctgc
     1501 atcagcgacg gctcggcgcc ttcttgcgcg acgctgtaat cctccggata aacgcgcccg
     1561 aaagtaaagc gctcgcgatt cttctgcgac gaagcgcggt agggataaag aatgtagccc
     1621 tcgtaaagaa cggcgttcac caccttctcg acgagcgcgg cattcatggc cgaaggagcg
     1681 gcgcgccggc ctggtcgccg gacgcggcct cggtctccgt ggtttccgat tcgttaggcg
     1741 gcggcagcag gcgctcgatc gtctgctccc aattcgcgag gctttcgcgc cggcggaagg
     1801 catagaggcg ctcaaagagg ccgcgttcga gcgcgagcca tgcggtattg ggataatggt
     1861 gatccatcat ctcctgccaa accgaaatcg gcatccgata ggcgcactcc ttgttccagg
     1921 aaacctgctg aatctggaga cgctcatccg gtccctcgta aaaaatcgtg ccgctaaaca
     1981 aaaagaggag cgggacctcg ccgccttcga gcgcgtaaaa atacttcgtg gccgagacat
     2041 tgagatcgta ggtgcacggc acggagagga ccgcttccgt cttgccggtg aattgcctga
     2101 cgttggcgct cgcatgtgtc cacaaacgcg cccgcaaggt ttgcccccag cgatcaggtg
     2161 tcccgaaaag ctcgcccaat ttttctttct ccgccggctg gtaggcccgc tgagtcgatt
     2221 ggatctggat ttgcgcctgc aacagtacgc tctggatcgt ctcggcctcc ggcgcgttgg
     2281 tgatttcgag tttgaaatgg aggagcggcg cgatcccgta gtccgccgct tcaacgccaa
     2341 caatcttaaa atcgaggtca ggcatcagtt tcctttcagg cgcgcgaaaa agccgtcgat
     2401 ctcgcgccag acatcttcgc cgccggagaa cccgcgccag tgaatccgga tgagaccgac
     2461 aagctcaaaa cagaggtcga tcggcgcgag gaaatattcg cgcgcgcctt cgacccgatt
     2521 gacgagcagc gtttccacat cggcctgcat atcgcgcagg accggattgg ccacgaccag
     2581 agcttcccag gccgtcaggg ggagaagcga ttcggtcgcg ccggccgggc tcggataata
     2641 ggcggccatc ttctcgttag gcgtatcatg gaaaaagaag gcgaggctga tcgggagcga
     2701 gagtccttcc cattcggcgt cgctcatctg gaaatccggg agcgcgcgcg cgtcccgcgg
     2761 gatgagcttg aattttccgc cgacgacatt ctcgaagcgc agcgcgcatg gatcgcagga
     2821 gcagacaatc cgccgggtcg ccatctcgag cagatgacga tgcgtcggcc cgagcggcac
     2881 actgcacaat tcgcaatgtt ccagctccgc tcggggagcc gcgatgggcg ttcgctgcgc
     2941 gctcgcaaaa cggcgcaaag tggcaaacga ggaggatctc attttcgata aatcttgtcg
     3001 ccgggggcgg tgaccccggt ttgctcgatg cctggttggg ccgggtccac gcccccagcg
     3061 acaactagga cggctgcaga ttcggcatgt cgggatgaat cggcgcccgc gcctccggat
     3121 tgatcccggt tgcgtcccgc gccgtggtat agctcttccc gccagcgccg cgccccgcct
     3181 ctttgttgtc gagagcgagt tcctcgcccc ttacggtccc gggtatatgt cctgctagtg
     3241 gtggtttgct catatgtttc ctcctgctcc tgctcttggt ccgctcccat tcgtcggcaa
     3301 cggacagtcg gtaaacttgg gaattggagt cgtcccgttc ttcgcgggtt tggggtgctc
     3361 ctcggggagc ccttcgacat cgaggccggt taaatccggc gccgcggcgt tgagcgcttc
     3421 ctcgatcgaa aagcgcaggg tcaggcgcga ggaaggacat tcgtgacaat tgccctgcag
     3481 ttttaattgc acccgacctt ccggtgtgac cgcgagtaat tccacactcc cgccatgcag
     3541 gccgagccgc ggtttcactt cctcgatcgc atttcgcacg cgtgtttcga gatcgagcgg
     3601 atgcaatcca tggagcaaca gcaaccccga gacgaggtga tcgcgggcca gttcatcgat
     3661 catcgccgcg ccctccgtcc cggaatcgaa gaccacactc aagacgcgct cgagtccggt
     3721 gccgtggagg tcgagcaaga tctgaatcaa ctcccgcacc tgctctcgcg ccgctggatc
     3781 gggtgtgcct tccagcgccg cgatgagttc ctctgctcgg ccgagctgtt tttggaactc
     3841 ctgtttgctg gtttcctgca cggatcctcc taactcatcg cgttctcgac tctatctttg
     3901 cgcggtttgc gtgcaacgtc accatttttg cggcaatcta actcccgtct cccgcaaacg
     3961 atacggcacc acgatggcga acggatgctt cagcgctcgc gtttgttttt agaaatgggt
     4021 ggaaaggcat tacacagcct ttggctgtag gttcgcggcg ccaaccgtta gaaattccat
     4081 tttcatgccg aaagctagcc taacgaacga cccgtttttc gttgcggtcg tcgccgcgat
     4141 tgaaaggaag attgccgaag gcgaccgcat cgcgcgcgca aacagcatca ccttgagcga
     4201 cgcgcagatt ttggcgctcc tgacaagagc cgcgggatcc gcgtgtggga aagcggcgaa
     4261 gaccagcccg ccccggcttc cgcgagatca aattctgact gctctggcag aacagctcgc
     4321 cctgctcagg gcgtccattt tcgaaaagaa aatcgaccgg gacggcgccg agacggaaac
     4381 gcccctctcc gccggacact ggatcgcggc gttaaaggtc gtgcaaaaat cgatccgccg
     4441 cgggacgggc catttgcgcg ggtctcgcag ttttctcgac tccctgagta tcttcatggc
     4501 ccgagccgca tcggccggca gcaagcgatg agcctggatt gccagggtta cgcgagcagc
     4561 cccaactcgc cgcgcaagcg atgcaattca gctacgtccc gctaaacgtg cgcggcgccg
     4621 gccgggagcg gtcgagcttc tggcattcca acgcttgcct ctccgcagcc cggccgcgct
     4681 cggctcgtta ggccaggaat tcatcgagcg cagcgccgag ccgctcgaga ccggcgcgga
     4741 gattttccgt ctcgccaccg atcccgattc ggaaatgtcg gggcgactcg aagaacgcac
     4801 cgggcacgac cgaggtctcg tatttctcgc gcagcagctt gaaaaacagt tccggttcac
     4861 cgcgcggcag gcgcgggaac accaccgtcc ccgcgggcgg acgaaaacat tccaggtcgg
     4921 cgcgcgaatc gagaaatgaa tcaagcagcg cgcggttcgg cgtgagcagc gcggcggtgc
     4981 gggcgcgaaa ccgcgcgagc tgatcaaacg ccacgaccga catgcgttcg gccgaatggg
     5041 cggcggtggc ggcgaagaga tcgtttagca accacatcct gcgcgccagc tccggtgcgg
     5101 ccaggatcca gccgcagcgc agtccactca ggccatacgc tttcgtcagg ctgctcgtca
     5161 cgacaaagtt ttcgcctaac gaaaaggagg catgatcctg gactcgccgc ggcgagtccc
     5221 ggagggacga gcttccgctc gtccctgccg tgccagggaa cagcatttcg agataaactt
     5281 catcgaccag cacgtgcgcc ccgacgcgtt gcgccatttc tccgatcgcg cgcaaggtct
     5341 ccgatcgaat cagggcgccg ctcggattgt gcaggttggt caggaccacg aggcgcgtcg
     5401 cgggcgtgat cgcctgcccg actgccggga gcgcgaggtc gaagttgttt tcgaaggtcc
     5461 gcgcgatccg tttcacccgc gcgccgaggt agtgcaggac atcgagcaag agcccataag
     5521 ttggttgttc gatcacgacc tcgtcgcccg gcgccaggag cgtggccatc gccaaatgat
     5581 tcgccatcga cgtcccttcg gccgtcacga tgcactccac cggcgcgccg gtgtgccgcg
     5641 caatccgctc gagcagcggc gcgtagccgt aaccgccgcc ggtgatctcc aattcctcca
     5701 gccgcaacgg aaattccgag agcagcacgt tcgttaggcc gctggtcgcg aggttgaagc
     5761 gcgcggtcga gcaggtcttc gcccactcca tgtagcgcga gcgtttggac tcctcgaggc
     5821 gattcattgc cgccaaacgt aaaccgcgga acgcatgttt tgtagggcgg gcgcttcgcc
     5881 tgccccggat ctcaacctgg gcaacccgcc tgaggcgggt cgccctacaa atccatctgg
     5941 cctcatttta gccggtcgcg gcgaatccgt ccggagagag gagcttccac tcgtccaaat
     6001 tgtgcgcggc caacccatgt cttatccgca ttcatctgta actcaaatcg actgacttat
     6061 ctccccgccg ccaaagaaaa taaatcggca atcccgcgag cgcgatcccg agtccaatgc
     6121 aggcgcgctt cggatcgtgc gcgaaggtgc tgaccacgat catccattgc gctgcgatga
     6181 agaggagcgt cgtccacgga tgccccggca cgcgataact ggccggcgcc ggcgctcgcc
     6241 gccgcaatac gaaaagacag accgccgtca gaccaaagaa gatgcaatcc atcgcgatga
     6301 cgtaattcac cacctgcgca taggtcccgg cgagcgcgat cacgatcgcc catccgccct
     6361 gcaacatgat cgccaccatc ggcacccgcg tgcgcggatg cactcgcgcc acgctccgaa
     6421 agaagacacc gtcccgcgcc atcgcgaaat aaacccgcgg cgcggtcagc atcgattgcg
     6481 cgaggaatcc gaaagccgaa atcgcgatgc cggcggcgat caactgtccg ccccgcgcgc
     6541 ctaacaaacc gcgcatgacc gaggaggcgg gcgtgctggt cgcggcgagg ccgctggcgc
     6601 ccagttcccg caggtaaaca aaattcaccg agagatagac cgcgatcacg cccagcaccc
     6661 cgaggatgag gccgcgcgga agcgttttgc gcgcgtcgcg cacttccccg gcgagaaagc
     6721 tcgtcgtttg ccagccgccg taggcgaaaa tgaccggcgt catcgccgcg ccaaatgcga
     6781 ccacgagatt cagcgaagcc ggctgatcca gcgccggacg ccacgcgatc cgcgacgtgg
     6841 cgccacgcca gaaactgacc gcctcgagga gcgcgatcgc cccgatcgcc gtcagggtca
     6901 aggtcgcttg cagtcggctg cccgcgcgca ccccgaggca gttgatcgcc gtcagcaatc
     6961 ccagcgccgc cgcggcgacg aacgcatcgg gcaacggcca ttgcgtcagc tcgaggaaat
     7021 agcgcgcaaa agttaccgcg accgccgcca ttccgccggt ctggataacg agcaacaaga
     7081 cccagccgta aaggaaagcc accgccggat gcagcgcctc acgcagataa gcgtactgcc
     7141 cgccgaccac cggcaaacgc gcagccagtt ccgcgtaaat gaatccgccg gccagcgcga
     7201 tcagcccgcc cgcgatccac gccccgagaa tgagcggcgc ggtgtggacc cgctgcgcga
     7261 cgagataagg attgatgaaa atgcccgcgc cgacgatccc gccgatcacc gccgtcgtcg
     7321 tgccgaaaag gccgagctgg cgggcgagtt tcccgtcttc gttaggcgtc gaagcgtggc
     7381 ctgctcccga agtcatgccc taacgaagac gggaatgccg aattccgaat gacgaatttc
     7441 gaaggaatga ccaaacccga atgacgaaga ggagcgggtc gcgggctccg tcattcgtaa
     7501 ttgtttcgtc atccaacatt cggaattcgt catttccccg tctcatccca ccatttggaa
     7561 agccgcgccg tcggcaccga gggcatcgag tactccgtgg tggaatagtg cgaggtcgaa
     7621 ttaaagagcg tgagccgggc gtgcgccgca tccttgaaat cgacgatgtt cagcgctgcc
     7681 gggctttgat cgagattgtc ccgataccgg cgcggatcga acccgaggag ggagctcagg
     7741 agcagccgga tcgtcgcctt atgcgaaacg atgatcgcgc actccccggg atgtgccctg
     7801 acgatctcca ccagcaccgg cagggcgcgc gccgtcaccg ccaggccgct ctcgccgccc
     7861 gccggcgcga aggtgtaggg atcttcgttc caggccgcgg cttcctcggg atatttctgc
     7921 tccacttcgc gccgcgtcat ttgctcccaa cagccgtgcg agatctcgcg caggccgtcg
     7981 cgcctgacga ccgggaggcc gtggggctgg gcgatgatgc tcgccgtctc caccgtccgg
     8041 cccagcgggg aggcatagac cgccgcgacc ttttcggcgc gcaaccgtt
//
LOCUS       DATMNS010000017        19167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4677211, whole genome shotgun sequence.
ACCESSION   DATMNS010000017 DATMNS010000000
VERSION     DATMNS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 19167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19167
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4677211"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VNW28_00640"
     CDS             <1..533
                     /locus_tag="VNW28_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08454.1"
                     /translation="NVVIGRDDVINAKHSTDDTLLALDRKTATTLPVRNLLPISTLDS
                     PLPLKVAVTRNGSKTIETNQPQVKGGAPSLPGLFYKGPAVDVPASRFVFGDDIGTSAT
                     PSPNDATVSQPMTDTPGSHLVLAGGNGGTVNVTSNGIVAVNSTIKVSASALPGRASNS
                     GGSGATTQPVGLAPGN"
     gene            536..1258
                     /locus_tag="VNW28_00645"
     CDS             536..1258
                     /locus_tag="VNW28_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA08455.1"
                     /translation="MSENSSNEPKTEHAEMIPAAASELNNLLQIIAGTAAMLENIWDG
                     TPGSEKYFEMLRLSVDRAAKVTAQLVKQVGGTSKKVLLSPDLATFAKGMPLSRTACGK
                     TRSIMVVDDEPMALLLAKQVLEQAGFDVVTAQSGFEALDLYRKHLGRFDLILLDLSMP
                     IMDGEETFNRLCAIDSQVVVLLNTGFIEKHRLDRMVAAGLAGFLRRPYQPSEVIEQIQ
                     SVLESVKRSRSNASPDAVAASS"
     gene            1269..2396
                     /locus_tag="VNW28_00650"
     CDS             1269..2396
                     /locus_tag="VNW28_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXA08456.1"
                     /translation="MTSLSPMAFDGLAKNRLFEGIEPDLLGQIAPDVQVMQLDAGAVV
                     FREGDRGDFLYLVGQGSIKISKSGRGGEQETLGFIQPGNFFGEMALLDGQPRSAMATA
                     VESTLLGTVNEPTFQHILELAPSRLHMNFLRSVTERLRSVNSHFISEVMRSERLSLVG
                     SMANSIIHDLKNPICIIRGCCDLIADHSTNPRQLELTKMMDNAVDGMLAMTQELLDYA
                     RGHTDLKCEEVSIGRLLDELNTQALGLLAGKNVLLVRDIRYKKGIRVDLVRFIRVLCN
                     LIKNAWEAMPNGGILTLSTELVEEQVVIRIADTGIGIPAEILSRVFEPFVTHGKSHGT
                     GLGMAIAKSIVSAHEGEISIASVLGKGTTAEIRLPLATDQE"
     gene            2476..3231
                     /locus_tag="VNW28_00655"
     CDS             2476..3231
                     /locus_tag="VNW28_00655"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HXA08457.1"
                     /translation="MDLADYLRASRRALIFTGAGISTGSGIADFRGPQGVWTRRQPVY
                     YQDFMTSEAARLEHWDYKLEAWEAFRQAQPNETHRAIVRLEQAGKLLLVVTQNIDGLH
                     GLAGTAPGQLVELHGTNSLVECQSCKRRSDPQPHFDFFRTRRQPPLCSCGGFLKPATI
                     SFGQSLDPAELERARAGALEADLVVALGSTLSVYPAAAFPLMIARRGAPYVIVNRGAT
                     EHDDESCVALRLEGEVSEIFPAAVEAALSSRAD"
     gene            complement(3343..5364)
                     /gene="tgt"
                     /locus_tag="VNW28_00660"
     CDS             complement(3343..5364)
                     /gene="tgt"
                     /locus_tag="VNW28_00660"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXA08458.1"
                     /translation="MTPSRLGFRLDALAKDSRARAATFRTLHNEVQTPLFMPVGTQAT
                     VKAQLTRTLEEAGTQILLANTYHLLLRPGAEVFRRTGGIHGFMSWDRAVLTDSGGFQI
                     FSLPHSRAMSEAGAVFQSYLDGQTILLSPELSVETQKAIGSDIMMALDQCIASNADEA
                     TARAAVEMTHRWAGRSLAARGDSPQSIFGIVQGALFPELRRESAGALGELPFDGFAIG
                     GLAVGESRSEREDLCEFTAQLLPADKPRYLMGVGTPLDILEAVHRGVDMFDCIMPTQL
                     AQRGAVFTSLGFLQMRRGVYKFSEEKLDPACACPTCARYSRAYLHHLTKTSETLGWQL
                     LGKHNIHFYHQLMRAIRESILAGRFAEFYREKRAVLQESDRANPMRHPRPARGKSLSL
                     GNYEVHIAHEGFASIRQVSSGEIMHLRTPPMEEACSLYVEQSGLAKRVCLTKTNPLVI
                     WDVGLGAAANSMAAIRCYEEQAALGPVRPMHIVSFENDLDSLRLALRHDDKFSYLRHG
                     GPAGILRRGQWQSKQHAGLSWQLVAGDFLETVAGAPAPPDLIFYDMFSSKTHREQWTI
                     EIFRRLFAACAGRATELFTYTHSTAARAALLAAGFYVAQGRPAGTKEETTIAFTPAAL
                     GSPFVRSYQLLAAEWVGRWNRSHAKFPAEVLADERLAFERQILEHQQFR"
     gene            complement(5404..6351)
                     /locus_tag="VNW28_00665"
     CDS             complement(5404..6351)
                     /locus_tag="VNW28_00665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HXA08459.1"
                     /translation="MIVTTKQLYKVAYGKFAVGAYNVNNMEQILGLFRGNMQSQAPFI
                     IQLSKGARKYADKRMLEALIRTAEQMFPDAIFAVHLDHGDEETCYDCIDSGFYSSVMI
                     DASHEPFAENVAITKRVVDRAHPKGISVEAELGQLGGVEEDIKVDEGHASLTNPEEAV
                     EFVRQTGCDSLAAAIGTSHGAYKFKGKQGLHFNVIEEIQKRIPGTPIVMHGSSSVPIE
                     EVERINAAGGQLDPSARGVNENEYLPAARLGVTKVNIDTDGRLVWTRVHREFFRDHPE
                     QFDFRPPGKIFVEEYANFIAHKNGKLGSANQLAAVRASL"
     gene            complement(6461..8461)
                     /locus_tag="VNW28_00670"
     CDS             complement(6461..8461)
                     /locus_tag="VNW28_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXA08460.1"
                     /translation="MILSAACILYTQDLNFVRRTRAFLRGLTPFRHVDQADRLDSVLQ
                     QNSPALLLLDLRARESRDLLAQVQVGWPNVLMIGFGMLRSDPLREAEQAGIYAVQEVD
                     LKRRDFQALVGRALDHLRVLEDNRAFRAEAPRMPAISNSRALEPAPEFSGPPLSILRF
                     PRVFRRFENTEMLVNSLVEGLADAAMVTRIGLFFRRAVGERYRLIGGLRCLPESHELE
                     YSERDPLVRWFELHAHLICRAYLAQTPDHAQRELLRRALDSVGAEVIVPLQARGRVSG
                     WLFFGHRLTGQPFDDQSLEALMILGDHASTVLENALLNEEVTLQKTLAETLLAAIPPG
                     IIASDEDAIIRWFNPSAESILGLAAGDVLNKPIEYVGSHIASLLRETLESTVPLPPKH
                     WIDPRNRRSLSVQTRRLIHARKPLGAVAVIQDRTPEESLRQRQELMDRAAFWTDLAAS
                     MSHEVRNPLVAIKTFAQLLPERFDDADFRRDFNEIVVQEIDRLDLIITQINNFAHPPE
                     LNFKSIDLRAPMKKAVELVRARSARNGFSLETTFPDDLPRILGDETALTEVFAHLVAN
                     AAEATHGQTKAKITLSAQPVREGDHTSGVVVTVRDNGTGITADLKDKIFSPFCTTKAR
                     GMGLGLPIVKRTVFDHNGRVDITSDPHGTSVKVLLPVVENEA"
     gene            complement(8458..10980)
                     /locus_tag="VNW28_00675"
     CDS             complement(8458..10980)
                     /locus_tag="VNW28_00675"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXA08461.1"
                     /translation="MTGASVSTSAAMLLQFSLGLAVFQANRRRLANQCFLLLSLAVVA
                     WLGSLLLAFGATTPQAAEFAIRQASAAGAVYLAILNFLRLSIRQRQLRWKGLLAHSKA
                     WLFLTILVVILCQTAAFLKGAVLSEEAHVPPRPEYGPAVWLYSIYIIAAFSALIISYW
                     HDIRNSKGAERVELSFIFIGAIAAVVSGLLLSFVLDYFLGPSRALWFAPFRMIGLSFV
                     MAYGIATRKILEVGFFMRRAISYGVLAVYLIALYALIFWLCYSVFVPLLGSDGLTLAH
                     VIASVVVAFAMAPARGVSRSLAERLFVGTRRLDFQATMNQATAILRSVTTLGDLLERF
                     GLTIGQAVDTERVFILLPEPDFYAQRYPAVQAGSRTTRVELHRDQAIIAHLERTQEPI
                     VVDELHRVPSTVELERVKRQMNSLQIAVAMGIFSRENLAGVMLLGARRSGLIYGKVEQ
                     DALRVLCGQLAVAIENAQLFTEVQNAKIYNETLLQNLTTGVVAADQNGLITVFNQEVE
                     QITGLPASELIARKISNLPDALRDILTETLSCGERQQDREIVLRRGDKNTILRASSSI
                     FHGQDNEPLGALMVLTDISMLKRLEQQIRRSDRLASLGTLSAGMAHEIKNPLVSIKTF
                     AQLLPERYQDSDFRETFFSLIGHEVDRIDSLVNQLLRFARPAKPLLKPMHVHEVLEKA
                     LLLVGHRLYQKDVKLTRSWQAEVDTIRADADQIEQVFLNFFLNALDAMKSGGSLTVAT
                     EIGSPETWPAALPGDSGDVQEILRITIADDGGGIKPEDVAHVFDPFFTTKDHGTGLGL
                     SVVHGIVQEHGGQIEVESELARGTSFHILLPLVRFQEEVAAA"
     gene            complement(11041..12285)
                     /locus_tag="VNW28_00680"
     CDS             complement(11041..12285)
                     /locus_tag="VNW28_00680"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXA08462.1"
                     /translation="MAKRNRVVVTGMGVIAPNGTGLKEFWRSLVSGKSGIGPITLFDA
                     TGFKSQIAGEVKDFNPLDSMNPQWKPQRMGRHTQFAYVATQMALKDADFDPKVGKLTT
                     VLPVCIGVSSSAFDVIENGFHELQARGAQRINAGIVRNCQPQAAALLIAQQLGVETQA
                     STISSACNSGIEAVASAVTMIGAGEAELAIAGGADAPVTPLAMASLTAAGLSSFANAE
                     PTRASRPYDVSSDSGVISEGCGVVILEDFDHAVARGARMYLEITGCAAQMDSDPDDPF
                     DGLEATMRLALANAGRGVREIDYICSYGPGHPVLDAAEVKVIRRVFLPEGHQVPVSSI
                     KGVTGNPLAAAGPMQLISCALAFQHNTIPPTANHEFPLPGCEWDFVSGKARKALLKCA
                     LINVRGMGGGNSSMVVERVRAT"
     gene            12510..13661
                     /locus_tag="VNW28_00685"
     CDS             12510..13661
                     /locus_tag="VNW28_00685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HXA08463.1"
                     /translation="MIANADLPQVLVIDDEMGPRESLRMLLKPSYQVHTADSVERGLQ
                     LLSEKKPDAIVMDIRMPGVTGIEGLRRIRQIDPHLSVIMLTGFGALETAKEALRLGAN
                     DYISKPFDAREMREVINRNVERTRLHRTSESAASEIKELNNRLLQELAQKERLASLGQ
                     ASAEFVHDIGNPLTIVWGYVQLLAKKLEESENGDSPNGVSSNKELEIIEQNVRLCRDL
                     LTMWQSYGSVEAAPHKLISISNIVREVVASVGAMAKEAAVELKCEVTENPCSLTGDAV
                     QITRAIQNVIINAIQACPASKGSVAMSCIRKDFYVDVCVADNGHGISSEQISKIFDPY
                     FTTKQGKSGTGLGLYITKKVVEDHNGSIKVDSTPQVGTTITIRLPLLNN"
     gene            13757..16174
                     /locus_tag="VNW28_00690"
     CDS             13757..16174
                     /locus_tag="VNW28_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08464.1"
                     /translation="MPDSNYRIAPIFLIRMAGVPFETLEKLATTETAQAARELLARQE
                     DFSRAKSDVEEFLAAYRRDLPKGQFRFWRKAIRSGVMPPAADPPSSVFARCWQSAEAV
                     ATAEAVLNDTLVRELAGARASLFAAARRYLPSYLVFAAQGVRERVVRQLASAAIALPP
                     RNKQARADERHLVLYLQRVAGKNDSLSEFGPEGWGTIASGDALLRLDPRAGIARRETF
                     LERWTAHGAAAAINSDPQTRAELTPRLHPDGRIEQNHFIHTATGEIAPLDPETLALLA
                     RCDGTTPAHSLGTTLATLEELARQNLIRWEMEVPALEPHAFDILVADVTGWRDGPVRA
                     RWLDELQPLAALPEKFARTTEPLSRLALIGEATERLEKLGAHKAASRFLYAASNPIGE
                     ECFRECNFSISEDLINQVAHEAAPWIDLWRDNYAFVARRVAAGLRGLLEKAPLQNGAL
                     PLPVFLQHCANLKMPLTGPGTIAFAHLAFQEVKEAFRAMMRARADAPECELTADDCQF
                     VRRNFPGESFDAFTYPSADLQLAAASRAAVARGDYQWILAELHPPPALLHHGFYWSCP
                     DKAALSDALEKTLFGQPNLHYGYFATDFTAATAVRFDALPRRTKFVASQRSAGNWQSY
                     RPAETEVFIDEKNGDVGVRTRDTREYLGSFARSWVIPLGFHPFNFSLGANTPRLRCGK
                     VVVQRRSWTVTLAELGHGDFTGVSRGLVAACERLRAMRQLPRYVYIRPAEQVLRRSGA
                     EGRDKDTKPVFIDLESYLFLEILHRWLVKAGELEVTEMLPAPDQLLWQEPDGRRVFEL
                     RTQIIPR"
     gene            16171..16947
                     /locus_tag="VNW28_00695"
     CDS             16171..16947
                     /locus_tag="VNW28_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXA08465.1"
                     /translation="MKVVAIANQKGGVGKTTTAVNLSAALAAQQTRVLLIDLDPQGNA
                     TSSFGLQGLEGQSLYGALLGDNPVTEKVLPTRLENLFIIPADLDLAGAEIEIARMDDH
                     LSRLAQALQPLRADETFDYVLLDCPPSLGILMTNALAAADELLTPIQCEYFALEGLVK
                     IVRLIEQIRASGANRELELGGILMTMFDSRTRLSAQVVADVRAHFGERVYETVVPRTV
                     RLSEAPSFGKCILEYDAHGSGAKAYGALAAEFLRRHPGTL"
     gene            complement(17097..17570)
                     /locus_tag="VNW28_00700"
     CDS             complement(17097..17570)
                     /locus_tag="VNW28_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08466.1"
                     /translation="MKTKSSLVIGTCIATLVLCAPASAPAKTKAAASPAPTASPAESA
                     AAMAKKPRPIPYHGKVASVDAGAKTFSLAGKESTRVIKITDQSTITKQGVAAAMADIV
                     ADEEVRGSYWKKEDGSLEARTVKLGPLTAEEMAKRDAHKKKKAAAAAEPSPSPAQ"
     gene            17742..19109
                     /locus_tag="VNW28_00705"
     CDS             17742..19109
                     /locus_tag="VNW28_00705"
                     /inference="COORDINATES: protein motif:HMM:NF022466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HXA08467.1"
                     /translation="MSGAECYAQLLPAAKVRERPLFPLPPSFTELELQARWFAGDFGK
                     SFIGTAGQEIEIVQFGTWNRETGPDFSDAAVRIDRGATSRGTIEFDLADRNWEVHGHA
                     TNPAFDETVLHVFVEAGERTFFSRTTANRNVPQLRVDPTTLPSAFSTNLPLARPGRCH
                     APLRGLPEEKIGRILDAAAQFRLQRKTLRLQRLSELHGDDEALFQELAGTLGYKENKL
                     PFTLLAQRLSRRTLRAQPDEIEAVLFGVGGFLMAPDLAKFEPGTRQYLRVLWDKWWPR
                     RAAFARLILPPRTWRLRGTRPLNHPQRRLAALAGIVAAWPPLVRSLGENDLSAARKFF
                     LDLAHPYWNFHYTLTSEPAPAEMALVGETRITEILANVILPFRQLRGGDVWPDYARLP
                     ARLTNRRLETGAARLFGDDPRRPRFLRTVARQQGLLQIYEDFCLQDDSDCAQCPFPEQ
                     MQKWT"
BASE COUNT         3764 a         5992 c         5754 g         3657 t
ORIGIN      
        1 ccaatgtcgt catcggacgc gatgacgtga tcaatgccaa acatagcact gacgacacgc
       61 tgctcgcgtt agaccgaaaa accgccacga cgttgccggt tcgcaacttg ttaccgatct
      121 caaccctgga ttcgccgctg ccactgaagg tcgcggtcac gcgcaacggg agcaagacga
      181 tcgaaactaa ccaaccccag gtcaaaggag gcgctccaag tctgcccggc ttattttaca
      241 aaggcccggc ggtggatgtc ccggcttccc ggttcgtctt tggcgacgac atcgggacct
      301 ccgcgacgcc cagccctaac gacgccacgg tcagccaacc gatgaccgac acgcctggct
      361 cccacctggt cctggccggc gggaacggcg gcaccgtgaa tgtcacgagc aacggaatcg
      421 tagcggttaa ttcgacaatc aaagtttccg ctagcgcgct gcctgggcgg gccagtaact
      481 ccggcgggag cggcgccact actcagccag tgggcctggc gcctggaaac tagagatgag
      541 cgagaacagc tcgaacgaac cgaaaaccga gcacgccgag atgatcccgg cggcggccag
      601 cgagctgaac aatcttctcc agatcatcgc cggcacggcg gcgatgctgg aaaatatctg
      661 ggacggaacg ccgggatcgg aaaagtattt cgaaatgctc cgcctgagcg tggaccgggc
      721 ggccaaagtg acggcccagc tcgtgaaaca agtcggcgga acgagcaaga aagtcttgct
      781 cagtcctgat ctggccacct tcgccaaagg aatgccgctc tcccgaacgg cgtgcggaaa
      841 aacgcgttcc atcatggtgg tcgatgacga gccgatggcg ctcctcctcg ccaaacaagt
      901 cctcgagcaa gccggcttcg acgtggtcac ggcgcaatcc ggctttgaag ccctcgatct
      961 ttatcgcaag cacctcggcc ggttcgacct cattctgctc gacctctcga tgccgatcat
     1021 ggacggcgaa gagaccttca atcggctttg tgcgatcgac tcgcaggtgg tcgtcctcct
     1081 gaacaccggg tttatcgaaa aacatcgcct cgatcgaatg gtggccgctg gtctcgcggg
     1141 ctttctccgc cgaccgtacc agccgagcga agtcatcgag caaattcagt ctgttctcga
     1201 gagcgtgaag agatcgcgca gcaacgcttc gcccgacgcc gttgcagctt cctcctagag
     1261 atcccatcat gactagtctg agtcccatgg ccttcgacgg gctggcgaag aaccggctct
     1321 tcgaaggcat cgagccagac ctgctcggcc agatcgcgcc cgacgttcag gtcatgcagc
     1381 tcgacgcagg tgcggtcgtt tttcgcgaag gagatcgcgg cgatttcctc tatttggtcg
     1441 gtcagggctc gatcaagatc tccaagtccg gtcggggcgg cgaacaggag accctgggct
     1501 ttattcagcc gggaaatttc tttggcgaaa tggcgctgct cgatggccag ccacgatcgg
     1561 cgatggcgac cgcggtggaa tccaccctcc tcggaacagt caatgagccg accttccagc
     1621 atattctgga gctcgcaccc agccgcctgc acatgaattt cctgcgctcg gtcaccgagc
     1681 gcttgcgcag cgtcaactcg catttcatca gcgaggtcat gcgttcggag cgactcagtc
     1741 ttgtcgggtc gatggccaac agcattatcc acgacctcaa gaatcccatc tgcatcatcc
     1801 ggggctgttg cgatttgatc gcggaccatt ccaccaaccc gcgtcaactt gaattgacca
     1861 agatgatgga caacgcggtc gacgggatgc tcgccatgac gcaggaactg ctcgactatg
     1921 cgcgcggcca caccgacctg aaatgcgagg aagtttcgat cgggcgtttg ctcgatgagt
     1981 tgaacacgca ggccctcgga ctcctcgccg ggaagaacgt tctgctcgtt agggacatcc
     2041 gctacaagaa gggcatccgc gtcgatttag tgcgcttcat tcgcgtcctt tgcaacctga
     2101 tcaagaacgc ctgggaagcg atgccgaacg gcggcatcct cactttgagc acggagctcg
     2161 tggaggagca ggtggtcatc cgcatcgccg acaccggcat tggaattccc gccgagattc
     2221 tgtcccgggt tttcgagcct ttcgtcaccc acggaaaatc gcacggcacc ggcctgggaa
     2281 tggcgatcgc caagtcgatt gtgagcgcgc acgagggaga aatctccatc gcgagcgtgc
     2341 tcggcaaagg caccaccgcc gagatccggc tccctctggc aaccgaccag gaataaggat
     2401 tcatcgccgg ccggcgcgat gagatcgccc gcgcatttgc caaacccccg tccgcacccg
     2461 caggataaac aacggatgga tctggctgac tatctccggg cttcgcgccg tgccctcatc
     2521 tttaccggcg ccggaatatc gaccggtagc gggattgccg attttcgggg accgcaaggt
     2581 gtctggaccc ggcgccagcc ggtctactac caggatttca tgacctcgga agcagcccgc
     2641 ctcgagcatt gggattacaa actggaagcg tgggaggctt ttcgccaggc tcaacctaac
     2701 gagactcatc gagcaatcgt caggctggag caggcgggca agctgctttt ggtggtaacg
     2761 caaaatatcg atggtctgca cgggctcgcg ggcaccgcgc cggggcagct ggtggaactt
     2821 cacggcacca actctctggt cgaatgccag agctgcaagc ggcgcagcga tccgcaaccg
     2881 cactttgatt ttttccgcac gcgtcgccaa ccgccgctct gcagctgtgg cggattcctg
     2941 aaaccagcaa cgatcagttt cggccagagc ctcgatcccg ccgaactcga gcgggcacgc
     3001 gcgggcgcgc tggaagccga tctcgtcgtc gccctcggct cgacgctttc ggtttatccg
     3061 gcggcggcct ttccgctcat gatcgcgcgg cgcggcgcgc cgtatgtgat cgtcaatcgc
     3121 ggcgcgaccg agcacgatga tgaatcctgc gtcgcactgc gcctcgaagg cgaagtgagc
     3181 gagatttttc cggcagcggt ggaagccgcg ttgtcgtccc gcgccgattg aaggcgccga
     3241 acggataagg cgccgatttc ggaagagcat gccgccgcgc taacttggcg ggaggtgctt
     3301 ccgcctgtcc gggacgcgcg gaagcgagtc ccccgaagtc gcctaacgaa attgctggtg
     3361 ctccagaatc tgccgttcaa acgccagccg ctcgtcggcg agaacttccg ccgggaactt
     3421 ggcgtgcgaa cgattccagc ggccgaccca ttccgcggcg agcaattgat aggagcgaac
     3481 aaacggcgaa cccagcgccg ccggcgtgaa tgcgatcgtg gtctcttcct ttgtgccggc
     3541 cgggcggccc tgggcgacat aaaagccggc cgcgaggagc gcggcccgcg ccgccgtcga
     3601 gtgggtgtaa gtgaaaagct ccgtcgcccg tcccgcgcag gcggcaaaca accgccggaa
     3661 gatctcgatc gtccattgtt cgcggtgcgt cttgctcgag aacatgtcgt agaaaatcag
     3721 gtcgggcggg gcgggcgcgc cagccacggt ttcgaggaaa tcacccgcca cgagctgcca
     3781 gctcaagccc gcgtgctgtt tcgactgcca ctggccgcgc cggagaattc ccgccgggcc
     3841 gccgtgccgg agataggaaa atttgtcgtc atgacggagc gccagtcgga gcgagtcgag
     3901 atcgttttca aagctgacga tatgcatcgg gcgcaccggc ccgagcgcgg cctgctcttc
     3961 atagcaacga atcgccgcca tcgagttggc cgccgcgccc agaccgacat cccagatcac
     4021 aagcgggttt gtcttcgtta ggcaaacgcg ctttgcgagc ccggactgct cgacataaag
     4081 actgcacgct tcctccatcg gcggcgtgcg caggtgcatg atttcgcccg aagaaacctg
     4141 ccggatgctg gcgaaaccct cgtgcgcgat gtgcacctcg tagttgccta acgagaggga
     4201 tttgccgcgc gccggccgcg gatggcgcat cggattggcg cggtcggatt cctggagaac
     4261 ggcgcgtttt tcgcggtaaa actcggcgaa ccggccggcc aggatgctct cccggatggc
     4321 gcgcatcaac tggtgataaa aatgaatgtt gtgcttcccg agaagctgcc agccgagcgt
     4381 ctcgctcgtc ttcgtgaggt gatgcaggta ggcgcgcgag tagcgcgcgc aggtcgggca
     4441 cgcgcaggcg ggatcgagct tttcctcgga gaatttataa accccgcgac gcatctggag
     4501 aaagccgagt gaagtgaaga cggcgccgcg ctgggcgagc tgcgtcggca tgatgcaatc
     4561 gaacatatcg accccgcgat ggaccgcttc gaggatgtcg agcggcgtgc cgactcccat
     4621 cagatagcgc ggcttgtcgg ccggcagaag ttgcgcggtg aactcgcaga ggtcttcccg
     4681 ctcgctcctg ctctccccga ccgcgagccc gccgatagcg aatccatcaa aaggcagttc
     4741 gcctaacgcc cccgcactct cccgccgcaa ttccggaaag agtgcgccct gcacaatccc
     4801 aaagatcgat tgcggcgaat cgccgcgcgc cgcgagactg cgccctgccc aacgatgcgt
     4861 catctcgacc gccgcgcgcg cggtcgcttc atccgcattc gaggcgatgc attggtcgag
     4921 ggccatcatg atgtcgctcc cgatcgcctt ctgcgtctcg acgctcagct ccggactgag
     4981 aagaattgtt tgcccgtcga gataactttg aaagaccgca cccgcttcgc tcatcgcgcg
     5041 cgaatgcggc agcgagaaaa tttggaatcc gcccgaatca gtcaggaccg cccgatccca
     5101 gctcatgaaa ccgtgaatcc cgccggtccg gcgaaaaact tccgcccccg gtcggagcaa
     5161 gagatgatag gtgttcgcga gcagaatctg ggtgcctgct tcttcgaggg tgcgagtcag
     5221 ttgggccttc accgtcgcct gggtcccgac cggcatgaag agcggcgtct gcacttcgtt
     5281 atgcaaagtg cgaaaggtcg cggcgcgagc gcgcgagtcc ttcgccagag cgtcgagccg
     5341 aaaacccaaa cgggaaggag tcatggaaag tgcgcggatg cggcctgcgc cctctcgcct
     5401 gctctagaga ctggcgcgca ccgcggcgag ttgattcgcc gacccgagct tcccgttttt
     5461 gtgtgcgatg aagttggcgt attcctcgac gaaaattttg ccgggcggcc ggaaatcgaa
     5521 ttgctcggga tgatcgcgga agaattcacg atggacgcgg gtccagacaa gccgtccgtc
     5581 ggtgtcgatg ttgaccttgg tgacgccgag cctggccgcg ggcaggtatt cgttttcgtt
     5641 caccccgcgc gcggagggat cgagctggcc gccagcggcg ttgatccgct cgacttcttc
     5701 gatcggaacg ctcgagctgc cgtgcatcac gatcggcgtg ccgggaatgc gcttctggat
     5761 ttcctcgatg acgttgaagt gcaggccctg tttgcccttg aatttgtagg cgccatggct
     5821 cgtgccgatg gcggcagcaa gtgaatcgca accggtctgc ctaacgaatt caaccgcttc
     5881 ttccggattc gtgagggagg cgtgtccttc gtcgacctta atgtcttcct ccaccccgcc
     5941 cagttggcca agttccgcct cgacgctgat gcctttcgga tgcgcacgat ccacgacgcg
     6001 tttggtgatc gcgacgttct ccgcaaacgg ctcgtggctg gcgtcgatca tcaccgagct
     6061 gtaaaagcca gagtcgatgc agtcgtagca ggtttcctca tcgccgtgat cgaggtggac
     6121 ggcaaagatc gcgtccggga acatttgttc ggcggtgcgg atcaacgcct cgagcattct
     6181 cttgtcggcg tacttccgcg cgcctttcga gagctggatg ataaacggcg cctggctttg
     6241 catgttgccg cggaacaggc cgaggatctg ctccatgttg ttcacgttat aggcgccgac
     6301 ggcgaatttc ccgtaggcga ctttgtagag ctgcttggtg gttacgatca tggctgaatg
     6361 gttgattgct gattagggcg tcgccaacct tatggcgctc tcggcagttt cccaatcaga
     6421 aatcaatcct tctcaggggg atccgcaatc agcaatgcac tcatgcttcg ttttccacca
     6481 cggggagcag gaccttgacc gaagtcccgt gcggatcgga ggtgatgtcc acgcggccgt
     6541 tgtggtcgaa gacagtgcgc tttacgatcg gtaggccgag gcccatgccg cgcgccttgg
     6601 tcgtgcagaa gggcgagaaa atcttgtctt tcaggtcggc tgtgatgccc gtgccgttgt
     6661 cgcgcacggt caccaccact ccgctggtgt ggtctccctc gcggaccggc tgcgcggaaa
     6721 gagtgatctt cgccttcgtc tggccgtggg tggcttcggc cgcgttcgca accaagtggg
     6781 cgaaaacttc ggtcagcgcg gtttcgtcgc ccaggattcg gggcagatcg tccggaaagg
     6841 tcgtctccag cgagaaaccg ttgcgtgcgg agcgcgcccg caccagctcg accgcttttt
     6901 tcatcggcgc gcgcaggtcg atcgacttga aattcagttc gggcgggtgc gcgaaattgt
     6961 tgatctgcgt gatgatgagg tcgaggcgat cgatttcctg gaccacgatc tcgttgaaat
     7021 cgcgccgaaa atccgcgtcg tcgaagcgct cgggcagcag ttgcgcaaag gtcttgatcg
     7081 cgacgagtgg attgcgcact tcatgcgaca tgctcgccgc gagatcggtc caaaaagcgg
     7141 cgcgatccat gagctcctgt ctttgacgga gcgattcttc cggcgtccgg tcctggatga
     7201 cggccaccgc gcctaacggc tttcgggcgt gaatgagccg gcgggtttgg acggagagcg
     7261 agcgccggtt gcggggatcg atccaatgct tgggtggaag cgggaccgtg ctctcgagcg
     7321 tctcacgcaa aagggaggcg atgtggctgc caacgtattc gatgggcttg ttcagcacgt
     7381 cccctgccgc cagaccgagg atcgattcgg cggacgggtt gaaccagcgg atgatcgcat
     7441 cctcatcgct cgcgatgatt cccggcggaa tcgcggccag gagcgtttca gcgagcgttt
     7501 tttggagggt gacctcttcg ttgaggagcg cattttccag cacggtggag gcatgatcgc
     7561 cgaggatcat gagggcctcg aggctctggt catcgaacgg ctgaccggtc agccggtgac
     7621 cgaaaaagag ccagccgctg acccgtccgc gcgcctgcaa cgggacaatc acttcggcac
     7681 cgacgctgtc gagcgcgcgg cggagcagct cgcgctgcgc gtgatcgggg gtctgcgcga
     7741 gataggcacg gcatatgagg tgggcgtgca gctcaaacca ccgcaccaga ggatcgcgct
     7801 cgctatattc gagctcatgg ctctccggca aacagcgcag cccgccaatc aagcgatatc
     7861 tctcgcccac ggcgcgccgg aaaaacaatc cgatgcgcgt caccatggcc gcatcggcca
     7921 ggccctcgac caggctgttg accagcatct ctgtattttc gaagcgacgg aagacccgcg
     7981 ggaaacgcaa aatcgagagc ggcggcccgc taaattccgg cgcgggctcg agggcgcggc
     8041 tattggaaat cgcaggcatg cgcggagctt cagcgcggaa tgctcggttg tcttccagaa
     8101 cgcgcaggtg atcgagggct cggccgacga gggcttggaa atcccggcgt ttcaaatcca
     8161 cctcttggac ggcataaatg ccggcctgct ccgcctcgcg caaggggtcc gaacgcaaca
     8221 tcccgaagcc gatcatcagc acattcggcc atcccacctg cacctgcgcg agcagatcgc
     8281 ggctctcccg ggcacgcaga tcgagcagga gcagagcagg gctgttttgc tggagcacgg
     8341 agtcgagccg gtcggcctga tccacatggc ggaacggcgt gaggccgcgg agaaaggcgc
     8401 gtgttcgcct aacgaaatta agatcctgcg tgtagaggat gcacgcggcg gataaaatca
     8461 tgccgcggcc acttcctcct gaaagcgaac cagggggagg agaatgtgaa agctcgtccc
     8521 gcgggcgagt tcgctctcga cctctatctg gccgccgtgt tcctgcacga tgccgtgcac
     8581 aaccgagagg ccgagaccgg ttccgtggtc tttcgtcgta aaaaagggat cgaaaacgtg
     8641 cgccacatcc tcgggtttga tccctccgcc atcgtcggca atcgtgatgc gcagaatctc
     8701 ctgcacgtcg ccggagtcgc cgggcagcgc ggccggccag gtctcggggg aacctatctc
     8761 ggtcgccacc gtaagggatc ctcctgattt catcgcgtcg agggcgttga gaaaaaagtt
     8821 aaggaaaacc tgctcaatct ggtccgcatc agcgcggatt gtatccactt ctgcctgcca
     8881 ggaacgagtt aatttaacat ctttttgata aaggcggtga ccaacgagaa ggagcgcttt
     8941 ttccagaact tcgtggacgt gcatcggctt gagcaatggt ttcgccggcc gggcgaaacg
     9001 gagcagttga ttgactaacg agtcgatccg gtccacctcg tgcccgatga gcgagaagaa
     9061 agtctcccgg aaatcggagt cctgataacg ctcgggcaga agctgggcaa aggtcttgat
     9121 tgagacgagc ggattcttga tctcgtgcgc cataccggcg gaaagcgtgc ccaggctggc
     9181 gagacgatcg ctgcgacgga tttgctgttc gagccgcttc agcattgaaa tatcggtgag
     9241 aaccatcagg gcccccagag gctcgttatc ctgcccatgg aaaatcgaac tgctggcccg
     9301 caaaatcgtg ttcttgtcgc ccctgcgcag gacgatttcg cgatcctgct ggcgctcgcc
     9361 gcagctgagc gtctccgtca aaatgtcccg caacgcgtcc ggcaaattgc tgatcttgcg
     9421 cgcgataagc tcggaggccg ggagtccggt aatctgctcc acctcctgat tgaagacagt
     9481 gatcagcccg ttttgatcgg ccgccaccac tccggtcgtg aggttttgca aaagcgtctc
     9541 attgtatatt tttgcattct ggacttcggt aaagagctgc gcgttctcga tcgcgaccgc
     9601 cagctgtccg caaaggacgc ggagcgcgtc ctgctcgact ttgccgtaaa taagacccga
     9661 gcggcgcgcg cccaggagca tgacgccggc gagattttcg cgggagaaaa ttcccatcgc
     9721 gacggcgatt tgcagggaat tcatttgtcg tttcacgcgt tccagctcga cggtggaagg
     9781 cacacggtgg agttcgtcga cgacgatcgg ttcctgtgtc cgttcaagat gggcaatgat
     9841 ggcttgatca cgatggagtt ccacccgcgt cgtccgcgaa cccgcctgca ccgcggggta
     9901 tcgctgagcg taaaaatccg gctccggaag gagaatgaag actcgctcgg tatcgaccgc
     9961 ctgcccgatc gttaggccga agcgttcgag cagatcaccg agcgtcgtga ccgaacggag
    10021 gatcgcggtc gcctgattca tcgtcgcttg aaaatcaagc cgccgcgttc cgacgaagag
    10081 gcgctcggct aacgaacgtg aaactccccg cgccggcgcc atcgcaaagg caaccacgac
    10141 ggatgcgatc acgtgcgcga gggtgagacc atcggagccc agcaacggaa cgaaaacgga
    10201 atagcacagc caaaaaatca aggcatagag cgcgatgaga taaaccgcca ggacgccgta
    10261 ggagatcgcg cgccgcatga aaaagccgac ttccaggatt tttcgcgtgg cgatgccata
    10321 ggccatcacg aaactgaggc caatcatgcg aaagggcgcg aaccagagag cgcgcgaggg
    10381 cccaagaaaa taatccagaa caaacgaaag cagtaacccg gaaacgacgg ccgctattgc
    10441 cccgatgaaa atgaaggaga gctcgacccg ttcagccccc ttcgaatttc gaatgtcgtg
    10501 ccaatagctg atgatgagcg cactgaaagc ggcgatgatg tagatcgaat aaagccaaac
    10561 agccggccca tactcgggac gcggtggaac atgtgcttcc tccgatagga ctgcgccttt
    10621 caggaaagcc gccgtctgac agaggatgac gaccaggatc gtgaggaaaa gccacgcctt
    10681 cgaatgcgcc aggagtcctt tccaacggag ctgccgttgc cggatggaga gacgcaaaaa
    10741 attaagaatc gcgagataga cggccccggc agccgaagcc tgccgaatgg caaactcagc
    10801 ggcctggggg gttgtagccc caaaggcgag gagaaggctg cccagccagg caacaaccgc
    10861 gagggaaaga agcaaaaagc actgattcgc gaggcggcgt cgattagcct gaaagaccgc
    10921 cagccccagc gaaaactgga ggagcatggc cgccgaagtg gaaacggagg cgcccgtcat
    10981 cttgccatac tcccagggaa taacatgaca ctcgctgatt gacgtgcccg agacgggcga
    11041 tcaagtcgca cgcactcttt ccaccaccat gctactatta ccgccgccca taccacgcac
    11101 attgattaaa gcgcatttca gtagcgcttt tcgggccttt cctgagacaa aatcccactc
    11161 gcagcccggc agggggaatt cgtgattagc agttggggga atcgtgttgt gctgaaaggc
    11221 gagggcacag gaaatcagct gcatcggtcc ggcggccgcc aacggattac cggttacccc
    11281 cttgatagaa ctcaccggca cctgatgccc ttccggtaaa aatacgcggc ggatcacctt
    11341 cacctcagcc gcatcgagca ccgggtgtcc gggcccgtag gaacagatgt aatcaatctc
    11401 ccgcacgccg cggccggcgt ttgccaaggc caggcgcatg gtcgcttcga ggccatcgaa
    11461 aggatcgtcc gggtcgctgt ccatctgcgc cgcgcaaccg gttatttcga gatacatcct
    11521 ggcgccccgt gccacggcat gatcgaagtc ctcgagaatc accacgccgc agccttccga
    11581 gatcacgccc gaatcgctgc tcacatcgta gggtcggctc gcgcgggttg gttcggcgtt
    11641 ggcgaacgag ctcaagccgg ccgcggtgag actcgccatc gccagcggcg taaccggcgc
    11701 atccgctccc ccggcaatcg cgagctcggc ctctccggcc ccgatcatcg tcaccgcaga
    11761 agcgaccgct tcgatgccgg agttacaggc ggaggaaata gtcgaagcct gggtttcaac
    11821 tccaagttgt tgcgcgatca gcagcgctgc ggcctgcggc tggcaattgc gcactatccc
    11881 ggcattgatc cgctgcgctc cgcgggcttg gagctcatga aagccatttt cgatcacgtc
    11941 aaaggcgctc gaactgaccc caatgcagac gggtaacacc gtcgttagct tgccgacttt
    12001 cggatcgaaa tccgcgtcct tcagagccat ttgggtggcc acgtaggcaa attgcgtgtg
    12061 ccggcccatg cgctgcggtt tccattgcgg attcatcgag tcgaggggat tgaaatcctt
    12121 tacctcgccc gcaatctggc tcttaaatcc ggtcgcatcg aaaagggtaa tcgggccgat
    12181 tccgctcttg cccgacacta acgaccgcca gaactccttc aggccggtgc cattgggggc
    12241 gatgaccccc atccctgtga caactactcg gtttcgcttt gccatccgtc ccccaatcgc
    12301 ctcccgccag cgttaagcaa ttcgtttcgc atggccatca gattctatcg attggcagcg
    12361 tttgtcgata aagttcaggt tattcacaat ttcgctcttg cctttcgcca cgaattacag
    12421 aagcatcgcc ctcgaagtga atccattttg gatcatttgc agattttccc ggcgaaaggc
    12481 acgtccctag cttctagcga tggggagcaa tgatagccaa cgctgatctc ccgcaagtcc
    12541 tcgtcatcga tgacgaaatg ggtccgcgcg aaagtttgcg gatgctgttg aagcccagct
    12601 atcaggtcca caccgccgat tcagtcgagc gcgggctgca actcttgagc gagaaaaagc
    12661 ccgacgccat cgtgatggac atccggatgc caggcgtgac gggcatcgag ggactgcgcc
    12721 gcatccgtca gatcgatccc catctttccg tcatcatgct gaccggattc ggcgcgctcg
    12781 agactgccaa ggaagcgctc cgcctcggcg cgaacgatta tatcagcaag ccatttgacg
    12841 cgcgcgaaat gcgggaagtg atcaaccgca acgtcgagcg cacccggctg caccgcacga
    12901 gcgaaagtgc ggccagcgaa atcaaggaac tcaacaatcg gcttctccag gaactcgcgc
    12961 agaaggaacg cctggcttct ctcggccagg cttccgctga atttgtccac gacatcggaa
    13021 atcccctaac gattgtctgg ggctacgtgc agcttctcgc caagaaactc gaagagtccg
    13081 aaaacgggga tagcccgaat ggcgtcagct ccaacaagga gctggagatc atcgaacaaa
    13141 acgtccggct ctgccgcgat ctgctcacca tgtggcagag ctacggcagc gtggaggccg
    13201 caccgcacaa gctcatttcc atttcgaata ttgtgcgcga ggtggtcgcc agcgtcggcg
    13261 ccatggccaa ggaagcggcg gtcgagctga agtgcgaggt gaccgaaaac ccttgctccc
    13321 tcacgggcga cgccgtgcag atcacgcgcg cgattcagaa cgtgatcatc aacgccatcc
    13381 aggcctgtcc cgcgagcaag ggctccgtcg ctatgagttg cattcggaag gatttttacg
    13441 tcgacgtctg cgttgccgat aacggtcacg gcattagttc ggagcagatc tcgaaaattt
    13501 tcgatcccta tttcacgacc aagcagggca agagcggcac cggcctcggg ctctacatca
    13561 ccaagaaagt cgttgaagac cacaacggtt cgatcaaggt cgacagcacc ccccaggtcg
    13621 gaacgacgat caccattcgg ttgccgttgc tcaataatta gaagtccctc aagcgctcct
    13681 ctccggcgcg cacgacagtc cgtttccttg gcggcattaa acattgccac cagcctctct
    13741 tctgttttct tcaggcgtgc cggacagcaa ttatcgcatt gcacccattt ttttgatccg
    13801 aatggcgggc gtgccgttcg agacgctcga gaaactcgcc acgacggaga ccgcccaggc
    13861 cgcccgcgaa ctgctcgcgc gccaggagga tttttcgcgg gccaaatcag acgtcgaaga
    13921 gtttctggcc gcctatcgac gtgatctgcc aaagggacaa tttcgcttct ggcgcaaggc
    13981 gatccgctcc ggtgtcatgc cgccggccgc ggatccgccc tcgagtgttt ttgcgcgctg
    14041 ctggcaaagc gcggaggcgg tggcgacggc ggaagcagtc ctcaatgaca ccctcgtccg
    14101 cgagctggct ggcgctcgcg cgtcgctttt tgccgcggca cgccgctacc tcccctccta
    14161 tctcgtcttc gcggctcaag gagtgcggga acgcgtggtc aggcagctcg cttccgccgc
    14221 gatcgcgctt ccgccccgca acaaacaagc ccgggccgac gaacgccacc tcgttctcta
    14281 cctccagcgg gtcgcgggca agaacgattc cttgagcgag ttcggcccgg aaggttgggg
    14341 cacgattgca tctggagatg cactcttaag actcgatcca cgggcgggca tcgcgcgccg
    14401 cgagacgttt ctggaacgtt ggaccgcgca cggcgccgcg gccgcgatca attccgatcc
    14461 gcaaaccaga gccgaactta caccccggct ccacccggat ggtcgcatcg aacaaaacca
    14521 ctttatccac accgcgaccg gcgaaatcgc gccgctcgat cccgagaccc tcgcgctcct
    14581 cgcccgctgc gatgggacga ccccggcgca ctcgttaggc acaacactgg ccacgctcga
    14641 agaactcgcc cggcaaaacc tgatccgctg ggaaatggaa gtgcccgcgc tcgagccgca
    14701 cgcgttcgac atcctggtcg ccgatgtcac cggctggcgt gatggccctg tccgcgcgcg
    14761 ctggctcgat gagcttcaac ccctcgccgc cctgcccgaa aagttcgccc gcaccaccga
    14821 gccgctctcc cgtctcgcgc tgatcggcga ggcgaccgag cgcctcgaaa aactcggcgc
    14881 gcacaaagcc gcctcgcgct tcctatatgc cgcgagcaat cccatcggcg aagaatgttt
    14941 tcgagaatgc aatttttcaa tcagcgaaga tctgatcaat caagtcgcgc acgaggccgc
    15001 accctggatc gatctctggc gcgataatta cgccttcgtc gcgcgccggg tggccgctgg
    15061 tttgcgcggg ctgctggaaa aggcgccgtt gcagaatggc gcgctcccgc tgcctgtctt
    15121 cctccagcat tgcgcgaacc tgaaaatgcc tctgaccggc ccgggaacga tcgcttttgc
    15181 ccacctcgcc tttcaggagg tgaaggaggc cttccgcgcc atgatgcgcg cgcgggccga
    15241 tgcgccggag tgcgaactga cggcggatga ctgccaattc gttaggcgaa attttcccgg
    15301 cgaaagtttt gacgccttta cctacccgtc agccgatctg caattggctg cggcctcgcg
    15361 cgcagccgtt gcgcgcggcg actaccaatg gattctggcg gaactccatc cgcctcccgc
    15421 cctcctccat catgggttct attggtcgtg tccggacaaa gccgccttat ccgacgcctt
    15481 ggaaaagact ctcttcggcc agccgaatct ccattacggc tacttcgcga ccgatttcac
    15541 cgcggcgacc gccgtgcgtt tcgacgcgct cccgcgccgg acgaagtttg tcgcttcgca
    15601 gcgttccgcg ggaaactggc agagctatcg tccggccgag acggaagtgt tcatcgatga
    15661 aaaaaacggc gacgtcgggg tgcggacgag agacacccgg gaatatctcg gttcttttgc
    15721 ccgcagctgg gtcattccac tcggttttca tcccttcaat ttttcgttag gcgcaaacac
    15781 cccgcgcctg cgttgcggga aggtcgtggt gcagcgccgc tcctggacgg tgacgctcgc
    15841 ggaactcggc cacggggatt tcaccggcgt ttcgcgcggc ctggtcgcgg cctgcgaacg
    15901 cctgcgagcg atgcggcaac tgccgcgcta cgtttacatc cggcccgccg agcaggtcct
    15961 ccgccggagc ggcgccgaag gacgcgacaa ggacacaaag ccggttttca ttgatctcga
    16021 gagctacctt ttcctcgaaa ttctccaccg ctggctggtc aaggccggcg aactcgaagt
    16081 caccgaaatg ttgcccgcgc ccgatcaact tctctggcag gaaccggacg gccggcgcgt
    16141 ctttgagctg cgcacgcaga ttattccgcg atgaaagtgg tcgccatcgc caatcaaaaa
    16201 ggcggcgtcg gcaagaccac cacggcggtc aatctctccg ccgcgctcgc cgcgcagcag
    16261 acccgggtgc tcctgatcga cctcgatcca cagggcaacg cgaccagttc tttcggactg
    16321 cagggactcg aaggtcaaag cctttacggc gcgttgttag gcgacaatcc ggtgacggaa
    16381 aaggtcctgc ccacccggct ggagaatctt tttatcattc cggcggacct tgacctggcc
    16441 ggggcggaga tcgagatcgc gcggatggac gatcacctca gccggctcgc ccaggcgctc
    16501 cagccgctgc gcgcggacga gaccttcgat tatgtcctgc tcgattgtcc accgtcgtta
    16561 ggcattctca tgaccaatgc cctggccgcc gccgacgagt tgctcacacc catccagtgc
    16621 gaatattttg cgctcgaagg gctggtcaaa atcgttcggc tgatcgagca gattcgtgct
    16681 tccggcgcga accgggaact cgagctgggc ggcatcctga tgacgatgtt cgacagtcgc
    16741 accaggctca gtgcgcaagt ggtcgcggac gtgcgcgcgc actttggcga acgcgtttac
    16801 gagacggtcg tcccgcggac cgtccggttg agcgaagcgc ccagctttgg aaagtgcatt
    16861 ctggaatacg acgcgcacgg gagcggcgcg aaagcctatg gcgcgctcgc cgcagagttc
    16921 ctgcggcggc accctggcac cctgtagacg cgtccttccg cgaagcgcga gctctgctgg
    16981 gacgtgaaga actgccgttt tttcgccgca acgccttcct ccgcgcttcg cagagcgaag
    17041 cgtcgacagc gcctactcgc cggtcgcagg tgtcgcttgc gttcgttagg gaaaaactac
    17101 tgcgcggggc tgggggacgg ttccgcggcg gcggcggctt tcttcttctt gtgcgcgtcc
    17161 cgcttggcca tttcttccgc cgtcagaggg cccagtttaa cggtccgcgc ttcgagcgag
    17221 ccgtcctctt ttttccagta cgagccacgc acttcttcgt cagccacgat gtccgccatc
    17281 gccgcggcca cgccctgttt cgtgatcgtg gattgatccg tgatcttaat cacccgggtc
    17341 gactccttgc cggcaagcga gaacgtcttc gcgccggcgt caacggaggc cactttgccg
    17401 tgatacggga tcggccgggg ctttttcgcc atcgccgccg cgctctccgc cggagaagcg
    17461 gttggcgcgg gagaggcggc tgctttcgtt ttcgccgggg cgctggccgg tgcacaaagg
    17521 acgagggtcg caatgcaggt gccgataact aacgaacttt ttgttttcat gttttctttt
    17581 tttgttggtg tctgacgagg caacattgcc tgcgctccgt ggacggcgat gcgccatccg
    17641 ttattcagcc cgtttattac catccggttg tttaggcgat gcaaatcttt ggcccgcttt
    17701 cccccttgcg gccgacgccg gccgcgctct agcctccccc catgtccggc gccgagtgtt
    17761 acgcgcagct tctccccgcg gcgaaggttc gcgagcgtcc gctttttcct ctcccgccca
    17821 gtttcaccga gctggagctg caggcccgct ggttcgccgg cgatttcgga aaatcgttca
    17881 tcggcacggc gggccaagag atcgagatcg tgcagttcgg cacctggaac cgtgagaccg
    17941 ggcccgattt ttcggacgcg gccgtccgga tcgatcgcgg tgcaacctcg cgcggcacca
    18001 tcgagttcga tctcgccgac cggaattggg aggtgcacgg ccacgcgacc aatccggcct
    18061 ttgatgagac ggtgttgcac gtctttgtgg aggcgggcga gcgcacgttc ttcagtcgca
    18121 caacagcgaa tcgtaatgtc ccacagctgc gggtcgatcc gacgacgttg ccgtccgcgt
    18181 tttccaccaa cctgccgctg gcccgcccgg gacgctgtca cgcgccgctc cgtggcttgc
    18241 cggaggaaaa aatcggccgc attctcgacg ccgcggcgca gtttcgcctg caacgcaaaa
    18301 cgcttcgctt gcaacgactg agcgagctgc acggcgatga cgaggcgctc tttcaagaac
    18361 tggccgggac actcggctac aaggaaaaca aactgccgtt caccctcctc gcccagcgcc
    18421 tttcccgccg aaccctgcgc gcccagcctg acgaaatcga agcagtcctt ttcggcgtcg
    18481 gcggatttct catggcgccc gacctggcca aattcgaacc cggcacccgc cagtacctgc
    18541 gcgtcctttg ggacaaatgg tggccgcgtc gcgccgcctt cgcgcggctc attctcccgc
    18601 cgcgcacctg gcgcctgcgc gggacgcgac cgcttaatca cccccagcgc cgcctcgccg
    18661 cgctcgccgg catcgtcgcc gcctggccgc cgcttgtccg ttcgttaggc gagaacgatc
    18721 tcagcgcggc gcgaaaattc tttctcgatc ttgcgcaccc ttactggaac ttccattaca
    18781 cgctcacttc cgagccggca cccgccgaga tggccctcgt cggcgaaacg cgcatcaccg
    18841 agattctggc gaacgtcata ctgcctttcc gccagctgcg gggcggcgac gtctggccag
    18901 attacgcccg gctgccggcg cgcctgacga accggcgcct cgagaccggc gcggcgcggc
    18961 tgttcggcga cgacccgcgt cggccgcgtt ttctacgaac ggtcgcgcgg caacagggat
    19021 tgctccagat ctacgaagac ttttgcttgc aagacgactc cgactgcgcg caatgtccct
    19081 tccccgagca gatgcaaaaa tggacgtgag gcaaaatccg aaagttcgga acggcttcac
    19141 tttgatcgag ctgctcgtgg tcatcac
//
LOCUS       DATMNS010000018         7370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_741386, whole genome shotgun sequence.
ACCESSION   DATMNS010000018 DATMNS010000000
VERSION     DATMNS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7370
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_741386"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1801)
                     /gene="rpoB"
                     /locus_tag="VNW28_00710"
     CDS             complement(<1..1801)
                     /gene="rpoB"
                     /locus_tag="VNW28_00710"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982917.1"
                     /note="DNA-dependent RNA polymerase catalyzes the
                     transcription of DNA into RNA using the four
                     ribonucleoside triphosphates as substrates; beta subunit
                     is part of the catalytic core which binds with a sigma
                     factor to produce the holoenzyme; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXA08468.1"
                     /translation="MSDRTATERIHFGTIKEAIEPPNLIEVQLNSYVDFLQKDVPPTK
                     RKNTGLQTVFKEVFPIESYDEKATLDFSQYEIGEPKLSALEAQREGQTFSAPLHVTFQ
                     LKEEKGTKEEKVYMGEIPLMTPSGTFVINGAERVVVSQLHRSPGIAFESSIHLNGKVL
                     YSFRIIPDRGSWIEVQFDTSDLLYIYLDRRKRRRKFLATTFLRALGYGTDEEVIKLFY
                     NIEDLKLSEKLDEESLATKVLTADVLDGEVTVARAFEPLTLAAVRQIMALGHKTVKVI
                     DITHDDTVVKALRKDPAHDQEEALKDIYRKLRPGDPPTVANARALLKRLFFDPKKYDL
                     GRVGRYKLNQKLGIDVDLGERILTDKDFIAAIKYLINLRRGEGTLDDIDHLGSRRVRT
                     VGELLANQCRVGLARTERLVKERMTLFDVNIDGMTPQKLINPKALSAVVRDFFGRSQL
                     SQFMDQTNPLAELTHKRRLSALGPGGLSRDRAGFEVRDVHPSHYGRICPIEMPEGPNI
                     GLISSMSTFARINEFGFIETPYRKVEKGRVTDQVDYLTGDREENFIVAQANAPMDSKG
                     HFTGEKVSVRYRGDFLEVEPAKVHYMDVSPKQLV"
     gene            complement(2103..2495)
                     /gene="rplL"
                     /locus_tag="VNW28_00715"
     CDS             complement(2103..2495)
                     /gene="rplL"
                     /locus_tag="VNW28_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXA08469.1"
                     /translation="MADMNAIVEQLSGLTVLEVAGLVKQLEEKWGVSAAAPVAMAAAP
                     AGGGGGGAPAAAAEEKTTFDVILKEMGANKIGVIKEVRAAVPGLGLAEAKALVEGAPK
                     TMKEGVTKEEAEEIKKKVEAAGAKVEIK"
     gene            complement(2670..3191)
                     /gene="rplJ"
                     /locus_tag="VNW28_00720"
     CDS             complement(2670..3191)
                     /gene="rplJ"
                     /locus_tag="VNW28_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXA08470.1"
                     /translation="MRPEKANIVSDLSEKLNRSPFLLVTDYQHMNVDHFGELRNRLAP
                     TGAEVHVVKNSFLKRAMTASGLPDVAEQLSGQTAVVTGDQDVAPVAKILKAFAAEFKI
                     AALKIGVVDRNVLSTAEVESLADLPSRAVLQAQLLGVLLAPATKLVRILNEPAAALAR
                     LLNAKTEKEGKPA"
     gene            complement(3204..3905)
                     /gene="rplA"
                     /locus_tag="VNW28_00725"
     CDS             complement(3204..3905)
                     /gene="rplA"
                     /locus_tag="VNW28_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HXA08471.1"
                     /translation="MQKNRSKRYRAAAEQVDRNKTYSFSEAVSTLKKLPPTKFDQTVT
                     MSFRLGVDPKQSDQMVRGTCPLPHGSGKQVRVLVFAQGAAAQAAKNAGAEHVGYEDLI
                     KKCQEGFQDFDVAIATPAAMSEVRKLGKVLGPRGLMPNPKTGTVTEDTAKAVGEVKAG
                     RVEFKLDKNGNVAVPLGKFSFAENQLVENGTAIIEAVVRARPATAKGRFVEGMTLAAT
                     MSPGLHVDPSPYLTI"
     gene            4028..4786
                     /locus_tag="VNW28_00730"
     CDS             4028..4786
                     /locus_tag="VNW28_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08472.1"
                     /translation="MKRNHWSILALVMLASTGVFSAAARPIEFSEVSLLVRAHENDAD
                     IMEQVRSRKLLHPLTAQQEDLLKSQGANDALLRSLRDSRFTLSSAQANAFTAKEQLAA
                     RNRPTASDQRGENDSLGENVQIFDVSYGHPVNLSRWGGPDQEIAFNCRRFAGEDLIEP
                     VLTDSGRTYVDTATYLGQGRPDDSTTIFDRRNYVSVMAHESSRPISIDTNNPVSIRGV
                     PYSLYPVYETRGVSLYYIGKSGGSVRLAVVLPKL"
     gene            complement(5069..5839)
                     /locus_tag="VNW28_00735"
     CDS             complement(5069..5839)
                     /locus_tag="VNW28_00735"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXA08473.1"
                     /translation="MKRKRLSRRQFLGLGLCAIPAAAGLDGRFVEPTWLRVTHFDHHP
                     QPTCRFVQFSDWHYKGDAEYAAKVVHTINALAPEFVCFNGDLIEDVRFAPAALDFIRQ
                     IEHPVYGCPGNWDYGSRADFREYEKAFHATGGAWLEDRSIVLPKHDLELIGVGARGAF
                     ALGEAKATRRVLLLHYPFQADRLGGRRYDLILAGHSHGGQVRIPFYGALVLPYGVGPY
                     QQGRYDSLGGPLYVNVGIGTYMFPVRFNCRPEITVVRM"
     gene            complement(5875..6900)
                     /locus_tag="VNW28_00740"
     CDS             complement(5875..6900)
                     /locus_tag="VNW28_00740"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXA08474.1"
                     /translation="MRAAALLLLAMIAAARAEDASLNLVLPTDNTALLRGDNPEFYQA
                     VERNLHGVISYPWQGGQYGFVRDPMELAGGIVYTRFHEGMDIRPLHRDARGEPLDEVR
                     AIADGKVVYVSRAAGASNYGRYVVVEHRWGGCPYYSLYAHLNTIAVEAGHAVHQGEKL
                     AIMGHTGSGINRERAHVHVELNLLLNDHFEQWHDMFFKNDPNHHGIYNGINLAGLDLP
                     RLFLALRKDPALTLPAFLGREEAFYKVIVPNTPNFQLPLRYPWMIASAPNEKPAAWEI
                     SFTRSGLPFRLTPSDQAVGGAELSYLKPGPIDARYLTRGNVNGRGQSARLTDSGKALM
                     RLLTFPD"
     gene            complement(6909..>7370)
                     /locus_tag="VNW28_00745"
     CDS             complement(6909..>7370)
                     /locus_tag="VNW28_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08475.1"
                     /translation="RRPLLAALVLLLATRADGGSPHCVFRVHVEANARDGAVFAQPIR
                     SLTGRNVFIEKTAWLTERDVKAYCPYRAADGSYGALLELDDHGRTVLDTLSVERRGAY
                     LYIFLNGRPLTELQVDRRVSDGKIYLASGLTEADIKSMNRDWKLIGGRKKK"
BASE COUNT         1422 a         2294 c         2139 g         1515 t
ORIGIN      
        1 agaccaactg tttcggcgac acatccatgt aatgaacctt ggccggctcc acttccaaaa
       61 agtcgccacg ataacggacc gaaacctttt cgcccgtgaa atgccccttc gaatccatcg
      121 gcgcattcgc ctgcgcgacg atgaaattct cttcgcgatc gccggtcagg taatcgacct
      181 ggtcggtgac gcggcctttt tcgactttgc ggtagggcgt ttcgatgaaa ccgaattcgt
      241 tgatccgcgc aaaggtgctc atcgagctga tgagaccgat gttcggacct tccggcatct
      301 cgattggaca gatgcgaccg tagtgcgacg gatgcacgtc gcgcacttcg aacccggcgc
      361 gatcgcggct tagaccgccg ggcccgagag cggagagacg acgcttatgc gtcaactcgg
      421 cgagcggatt cgtctgatcc atgaactgcg aaagctgcga acggccgaaa aagtcgcgca
      481 cgaccgcgct gagcgccttc ggattgatca acttctgcgg cgtcatgccg tcgatgttca
      541 cgtcgaacaa cgtcatgcgc tctttcacca gccgctccgt gcgggcgaga ccgacgcggc
      601 actgattggc gagcaattcg ccgaccgtcc ggacgcgacg gctgccaaga tgatcgatat
      661 catccagcgt gccttcgcct cggcgaagat tgatcagata tttgatcgcg gcgatgaaat
      721 ccttgtcggt caggatgcgt tcgccgagat cgacgtcgat gccgagcttt tgattgagct
      781 tgtagcgacc cacgcggccg agatcgtatt ttttcgggtc gaaaaacaac cgcttgagca
      841 gggcgcgcgc gttcgccacc gtgggtggat cgccggggcg caacttgcga taaatgtcct
      901 tgagcgcctc ttcctgatcg tgcgccggat cttttctcag cgccttcacg accgtgtcgt
      961 cgtgcgtgat atcgatcact ttgaccgttt tatgtccgag cgccatgatc tggcgcacag
     1021 cggcgagagt gagcggttcg aaagcgcgcg cgaccgtcac ttcgccatcg agcacgtcgg
     1081 ccgtgagcac cttggtggcg aggctttcct catcgagctt ctcgctcagc ttgagatcct
     1141 cgatgttgta aaaaagtttg atcacctctt cgtccgttcc gtagcccagg gcgcggagga
     1201 aagtggtggc gaggaatttc cggcgacgtt tgcggcgatc gagatagatg taaagcagat
     1261 cgctggtgtc gaattgcact tcgatccagg agccgcgatc cggaatgatc cggaagctgt
     1321 agagcacctt gccgttgagg tggatgctcg actcgaaagc gatgcccggg gagcgatgca
     1381 attgcgagac gaccacgcgt tcagcgccgt tgatcacgaa cgtgcccgac ggcgtcatga
     1441 gcgggatttc gcccatgtaa accttttcct ccttcgtccc cttttcttcc ttgagctgga
     1501 aagtgacgtg taacggtgcg ctaaaggttt gcccttcgcg ctgcgcttcg agcgcgctca
     1561 gtttcggttc gccgatttca tactggctga aatcgagcgt cgctttttcg tcgtagctct
     1621 cgatcgggaa aacttctttg aacaccgttt gcagaccggt gtttttccgc ttggtcggcg
     1681 ggacgtcttt ttgcaaaaaa tcaacgtatg agttgagctg gacctcgatc aggttgggtg
     1741 gctcgatcgc ttctttgatg gtcccgaagt ggatacgctc tgttgcagta cggtctgaca
     1801 tatctggtgc accaaatctc caatgatttt aaaacctgca acgaccctgg atgcaggtcg
     1861 cgaggagtct gagggttacg ggcttggccg gaaatcgcgg gcggaaaaag tggcagcaaa
     1921 acggcggtct gtcaacgcga cctccaccgt tttgttgcca atcttgagat atgccttcga
     1981 gagcgggtta aaaatacgtc gggcgactcc tcgccacaga aaaaagggag gaataagtag
     2041 ccacatcctg ccggggagcg caagcgcttt ttgtcccgcg ccgcgcgccc cagggatgta
     2101 aactatttga tctcgacctt ggcgcccgcc gcttcgacct ttttcttgat ctcttcggct
     2161 tcttccttgg tcacgccctc cttcatcgtc ttgggcgccc cctcgaccag agccttggct
     2221 tccgccagtc cgagtccagg gaccgcggcg cgcacttcct tgatcacacc gatcttgttc
     2281 gcgcccattt ccttcaggat cacgtcgaaa gtagtcttct cttcagccgc tgccgcggga
     2341 gctccgcccc caccgccgcc agccggcgcc gccgccattg ctacgggagc cgccgcgctg
     2401 acgccccatt tttcttcgag ttgcttcacc aatccagcaa cctcgagaac ggtcagtccg
     2461 ctgagttgct ccacaattgc gttcatatct gccatttttg tttcctccaa ttgccgctcc
     2521 cgaaagcccc cgggaaatta cagccgatag ccatcggctc ggacaggttg ttcttgtttg
     2581 ttgtagggca attcgcctga ggcaaattgc cgcggcaggc gacgcgcctg ccctacaaaa
     2641 atttgttcgt taggccgcgg cctcccgcct caggcgggct tgccctcctt ctcggttttg
     2701 gcattcaaca gccgcgccag ggccgcggcc ggttcgttca aaattcggac cagcttcgtc
     2761 gccggggcca gcagcacgcc cagcaactgc gcctgcaaaa cggcgcgcga tggaagatcc
     2821 gccagcgatt ccacctccgc ggtcgaaagc acattcctgt cgaccacgcc gattttgagc
     2881 gcggcgatct taaactcggc cgcgaacgct ttcaaaatct tcgccactgg cgcgacatcc
     2941 tggtcgcccg tgaccacggc ggtctgcccg ctcagttgct ccgcaacgtc cggcagtccg
     3001 ctcgccgtca tcgcgcgctt gagaaagctg ttcttgacca cgtgcacctc agcgccggtc
     3061 ggcgcgagac gattccgcag ctccccgaag tgatcgacgt tcatgtgctg gtagtcggtc
     3121 acaagcagga acggcgaacg attcagtttc tcggacaggt ccgaaacgat gttggctttt
     3181 tcaggtctca tggtgcaaaa aaatcagatt gtaaggtagg gtgaaggatc gacatgcagc
     3241 ccgggtgaca tcgtcgcggc gagcgtcata ccctcgacga aacggccttt ggccgtcgcc
     3301 gggcgtgccc ggaccacggc ctcgatgatc gcggtgccgt tctcaaccaa ctggttttca
     3361 gcgaaggaaa atttgccgag cggcaccgcc acattgccgt tcttgtcgag cttgaactcg
     3421 actcgcccgg ccttcacctc accgaccgct ttggccgtgt cttcggtcac cgtcccggtt
     3481 ttcggattcg gcatcagccc gcgcgggccg agcactttgc cgagcttgcg cacttccgac
     3541 atcgccgccg gagtcgcgat ggcgacatcg aaatcctgaa agccttcctg gcatttcttg
     3601 atcaaatctt catagccgac atgttccgca ccggcgttct tcgccgcctg cgccgccgcg
     3661 ccctgggcga aaacgagcac gcgcacctgt ttgccgctgc cgtgcgggag cggacacgtg
     3721 ccacgcacca tctggtccga ctgcttcggg tcgaccccga gccgaaacga catcgtgacc
     3781 gtctggtcaa acttggtcgg cggcagtttc tttaacgtgc tgaccgcctc ggagaaactg
     3841 taggtcttgt tgcgatccac ctgctcggcg gccgcgcgat aacgtttgct gcgatttttt
     3901 tgcatgatga tttcagtgcc agcgagccgg gtggctctcc tgattactcg tctggagcga
     3961 gggacgagga agttagcggc atttcatcgc ttgtcaaaac cgaaaccgtt ttgctacaat
     4021 cgcacccgtg aaacgaaatc actggagcat ccttgcttta gtcatgctcg cttccacggg
     4081 cgtcttttcc gccgcggcgc ggccgatcga gttcagcgag gtctcgctcc tcgtccgcgc
     4141 gcacgagaac gatgccgaca tcatggagca agtgcgcagc cgaaaacttc ttcatccgct
     4201 caccgcccag caggaagacc tgctgaaatc gcagggggcg aacgatgcac tcctgcgcag
     4261 cctgcgcgat tccagattca ccctttcatc cgcgcaggca aacgccttca ccgccaaaga
     4321 acagctggcc gcccggaacc gcccgactgc ctctgaccag cggggggaga acgattcgtt
     4381 aggggaaaac gtgcaaatct tcgacgtttc atacggtcat ccggtcaatc ttagccgttg
     4441 gggtggaccc gaccaggaga tcgcctttaa ttgtcgccgc ttcgctggcg aagacctgat
     4501 cgagcccgtc ctcaccgaca gcggccgcac ttacgtcgac accgccacct acctcggtca
     4561 gggccgcccc gatgacagca ccacgatctt cgaccggcgc aactacgtct ccgttatggc
     4621 ccacgagtcc tcccgcccca tctccatcga cacgaacaat cccgtctcga tcaggggcgt
     4681 gccctactca ctctaccccg tctacgaaac gcggggcgtc agcctttatt acatcggcaa
     4741 atctggcggc tccgtgaggc tcgccgtcgt tcttccaaag ctctaatccg cccgaaacgc
     4801 gatcggttcg cgtgcacttc acttcttcgg gttaatgcac gtgatcgtgg gcggaataca
     4861 agaaagaaga aaaggcgctg gaggaagcga agtatacccg gcttcgttct cgatagcttc
     4921 gcgctcatcg cctgtttccg cgatgaagcg gcgagaacaa actcgaaaat ctgctgcaca
     4981 aggcagcgac ctcgtcatca gcgatcatga attggcgatc gtttccgagc acgttgaaag
     5041 aatctcaggc cgcgagccag cgcctgtcct acatcctgac gaccgtgatc tccggccggc
     5101 aattgaagcg caccgggaac atgtaggtgc cgatgcccac gttgacatag agcgggccgc
     5161 ctaacgaatc gtaacgtccc tgttgatagg gacccacgcc gtagggcagc acgagggcgc
     5221 cgtagaacgg gatccgcacc tgcccgccat gcgaatgacc agcgaggatc aagtcgtagc
     5281 gccggccgcc gaggcgatcg gcctggaaag gatagtgaag cagcaaaacc cgccgcgtcg
     5341 ctttcgcttc gcccaaggcg aaggcgccac gcgctcccac gccaatcagt tcaagatcgt
     5401 gcttcggcag aacgatactg cggtcttcga gccaggcgcc gccggtggcg tggaacgctt
     5461 tctcgtactc gcgaaaatcg gcccggctgc cgtaatccca attgccggga caaccgtaaa
     5521 ccgggtgctc gatctggcgg atgaagtcga gcgctgcggg cgcgaaacgc acgtcttcga
     5581 tcagatcgcc gttgaagcag acaaactccg gtgcgagcgc gttgatcgtg tgcacgactt
     5641 tggccgcgta ctcggcgtcc cctttgtaat gccagtcgct gaactgaacg aaacggcagg
     5701 tcggttgggg atggtgatcg aaatgcgtca cgcgcaacca ggtgggttcg acaaaacggc
     5761 catcgaggcc ggctgccgcc ggtatggcgc agagtccgag cccaaggaat tgccgccggc
     5821 tgagcctttt ccgcttcaac cctttaaccc ttcaccccat ccggttcgca ttcgttagtc
     5881 cgggaaggtc agcaggcgca tgagcgcctt cccgctgtcg gtcaggcgcg cgctttggcc
     5941 ccgaccgttc acgttgcccc gggtcaggta gcgggcatcg atcggacccg gcttcagata
     6001 ggaaagctcc gccccgccga ccgcctggtc gctcggcgtg aggcggaaag gcaatccgga
     6061 acgggtgaag gaaatttccc aggccgccgg tttttcgtta ggcgcagaag cgatcatcca
     6121 cggataacgc agcgggagct gaaagttggg cgtatttggg acgatcactt tgtagaaagc
     6181 ctcttcccgg cccagaaacg cgggcaaggt gagcgccgga tccttacgca aagccaggaa
     6241 gagccgggga aggtcaagtc cagcgaggtt gattccgtta taaatcccat ggtgattcgg
     6301 atcgttttta aaaaacatgt cgtgccactg ctcgaaatga tcgttcagca ggaggttcag
     6361 ttccacgtgc acatgcgcgc gctcgcggtt gatgccgctc ccggtgtgcc ccatgatggc
     6421 gagcttttcc ccctggtgca ctgcgtgtcc ggcctcgacc gcgatcgtgt tgagatgcgc
     6481 gtagagactg taatatgggc aaccgcccca gcgatgctcg accacaacgt aacgcccgta
     6541 gttggaagcg cccgccgccc ggctcacata gacaaccttg ccgtccgcga tcgcgcgcac
     6601 ttcgtcgagc ggttcgccgc gcgcgtcgcg gtgcagcggc cggatatcca tcccctcgtg
     6661 gaagcgcgta tagacgatcc caccggccag ttccatcgga tcgcgcacga aaccgtactg
     6721 cccgccctgc caggggtagg agatcacacc gtgcaggttg cgttcgacgg cctgataaaa
     6781 ctccgggttg tcgccgcgca gcagcgctgt gttgtcggtc ggcaggacga gattcagcga
     6841 cgcatcttcg gcccgcgcgg cggcaatcat ggcgagcaag agcagcgcag cggcgcgcac
     6901 ccaacccctc atttcttctt ccgcccgccg atcagcttcc aatccctgtt catcgacttg
     6961 atgtcggcct cggtcaggcc ggaagcgagg taaattttcc cgtcggagac ccgccgatcc
     7021 acctgcaact cggtcagcgg ccggccgttc aggaagatat agagataagc gcctcggcgc
     7081 tcgacgctca aagtgtcgag gacggtgcgc ccgtgatcgt ccagctcgag gagcgcgccg
     7141 tagctgccat ccgcggcgcg atacgggcag tatgccttga cgtcgcgctc ggtcagccag
     7201 gcggtttttt cgatgaagac gttgcggccc gtcagcgagc gaatcggctg cgcaaaaacg
     7261 gcaccgtccc gggcattcgc ctcgacgtgg acgcggaaga cgcaatgcgg cgacccgccg
     7321 tcggcgcgcg tcgccaggag cagaacaagc gcggcgagga gcgggcgtcg
//
LOCUS       DATMNS010000019         9946 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4309942, whole genome shotgun sequence.
ACCESSION   DATMNS010000019 DATMNS010000000
VERSION     DATMNS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4309942"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VNW28_00750"
     CDS             complement(<1..363)
                     /locus_tag="VNW28_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08476.1"
                     /translation="MRRAFFCLLVGVATLMAAPQPARAGKPGRRGKQVWSGLVVATNS
                     ARPRPAPVEMRHLDPTLRRTFGYNQFELIGVSRKDLQNGEVNWLASSKHFSLRVDPRG
                     VSSDGYRVNLQLFQDRQVL"
     gene            complement(367..888)
                     /locus_tag="VNW28_00755"
     CDS             complement(367..888)
                     /locus_tag="VNW28_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08477.1"
                     /translation="MKTFEEKWTAWVDDQLSDAERAEFEASLGDRAAAQAEKHQAHKL
                     GALLKEELQPRAMGNEEFFHLQLRNRIAAESERPATRPAPQSRENWWTIRRLLWTGAS
                     SLAVFAICTFFVMRQEQAPDQSQYLTQILNARVDPGVSPYATVSIFQTKQDRVTVLWV
                     DGLKALPSDYASK"
     gene            complement(875..1513)
                     /locus_tag="VNW28_00760"
     CDS             complement(875..1513)
                     /locus_tag="VNW28_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXA08478.1"
                     /translation="MNRASTRLKVDTETTAAPADVPELDLVKQAQAGDTEAFDQLVSR
                     FRNRVFGMIYNMVHNEQDAWDLAQDSFLKAWKSIARFRGQSSFYTWIYRIVMNVTIDW
                     LRKKQVQGAGAEFDDAIQLTEIDPASRTVPHAGALPHERMEQKEIRVRIDAAIAQLSP
                     EHRAVILMKEIEDMQYHEIAESLGCSIGTVMSRLFYARKKLQNLLRDVYENV"
     gene            complement(1521..2180)
                     /locus_tag="VNW28_00765"
     CDS             complement(1521..2180)
                     /locus_tag="VNW28_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08479.1"
                     /translation="MAEHDSDRAWDEYEWERFLQQQDQKTEKYMELLEKYIDDPQRDE
                     IVAREMGWCHLNEDNGEDWNDDADPLLAENDEEGDEDSALNLINECAESFEQHVLYRA
                     AFSLTIWIDRLFEANPALQNDPAAVKLATHTALASAKLAAALSDEEAEELGMTIAYLK
                     RSLKAITVAMDAGAQLIGEKSLTRKQHAALQQRLFQVRDGIILLMGEFRGEWLRRYGS
                     L"
     gene            2293..3999
                     /locus_tag="VNW28_00770"
     CDS             2293..3999
                     /locus_tag="VNW28_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXA08480.1"
                     /translation="MRTIDADPDEQFYRDTEAVAFPKIDDRQLALLEPLGKRRTLRRG
                     EMVFKAGQRDLPLTVILRGEIEVFEKREETEQILGTPGVRDFVGDVAMLQGTSALASA
                     RVKSDEAEILQVPAAELRRALVELPGVGEPVVNAFIMRRQRLMRDREFAGLRVIALRD
                     TREGHQLHDFLAKNHVPHRLIEFEGEPGQALARRFNLTSRDLPALIAATGAPWRRPSL
                     REVAQVAGLLRPLTQGDESEIHCDLAIVGAGPAGLAAAVYAASEGLKTVVLDGYAPGG
                     QAGSSSLIENFFGFPTGISGGDLTYRAQLQAYRFGAKFSTPSQALALSFHDGEEHRAS
                     LQIEGCFATLRSKCVLISTGADYRRLDAEGREEFENSGVYYAATALEGQLCGGATVVV
                     AGAGNSAGQAAMYLSEGAAKVLLVVRGDDLRKSMSDYLSRRVEACQNIEILHRTEIRK
                     MTGGKMLEKVELEQLETGERRTVETPAVFSMIGAKPCTNWLPPEIERDEKGFIKTGTA
                     VAAHPAWQKSKRPPGPLETSMPGIFAAGDVRSGSVKRCAAAVGEGGMAIAGVQAALAS
                     PA"
     gene            complement(4006..5103)
                     /gene="rlmN"
                     /locus_tag="VNW28_00775"
     CDS             complement(4006..5103)
                     /gene="rlmN"
                     /locus_tag="VNW28_00775"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HXA08481.1"
                     /translation="MAAPLDSLVAKPAIKSLTPEELTPHFAARGEPAYRAKQVADWLY
                     RKRVDSFAAMTDLPAATRARLAEEFSFVRPEIVQMLGSKDTTRKYLFRLADGNLIESV
                     LIPASPALYGAASDRRTACVSTQVGCAYGCRFCASGLDGFTRNLEPDEIVDQLLAIEA
                     ASGETIDNIVFMGMGEPLANLTNLLRAIRIINAPWGLEIGARHITISTSGLAPQIRKL
                     ATEPTQFRLAISLHGATDEVRSKIMPVNRKYNLATLLAACADYVAQKKRLMFEYILIA
                     GVNDTDEQAHALARHAACLSAKINLIPYNTVEGLAWTRPSRNRQESFLRILREHGAVA
                     TLRREKGHDIDAACGQLRLQTKRRERQPVTA"
     gene            complement(5109..5879)
                     /locus_tag="VNW28_00780"
     CDS             complement(5109..5879)
                     /locus_tag="VNW28_00780"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXA08482.1"
                     /translation="MARAVHIFDNGVRVYEDHLLDEQRARYEKRNVHEAEEEEIFVDL
                     IRRLPPGGCFVDIGAAIGYYLILARTLAPRLTIHGVEPLERHRRFLRENMTLNGLTND
                     DLTLHAEGLNSSAGSETLMDRGYSSRLAAVAGERTLSPSARWKRFLEGVGLRRPRNRT
                     VTIATITLDDFVRRIGRPIDLLQMDVQGLEVEVLKGGTEAMKAGAVKTFLIGTHGRAR
                     GLTLHDDCRELLRASGYKIEIDLPDTKEQPDGILVAGK"
     gene            complement(5965..6828)
                     /locus_tag="VNW28_00785"
     CDS             complement(5965..6828)
                     /locus_tag="VNW28_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08483.1"
                     /translation="MKPGTLTHTIAVLLGSASAVFAPSAPAQPVTAKFKLSKKQNRLN
                     EDVLLKVDPIGYTPPGVAGIPTRKGAERFPWKKDIVTTVFWIGENPTANNPVPNHASS
                     WDADWAKDYGGYDDPNPARRQNYIPVKFTPRQNPFYCALPYNDKSREGHRPEAARVIP
                     WFNEAYQGPGVSVCKGRWVAIHKGDRTVYAQWEDAGPFRTDHWEYVFGNERPKSNLNR
                     GAGLDVSPAVRDYLGLQDTDVTDWKFVDFNEVPPGPWAKLGDNNTFVINQRKAQQELA
                     KAKGKSATIVR"
     gene            complement(7078..8445)
                     /locus_tag="VNW28_00790"
     CDS             complement(7078..8445)
                     /locus_tag="VNW28_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXA08484.1"
                     /translation="MSLAAKLRALLGGEIVAEDEATRIVHGGDKWFAHHEPEVVVFAR
                     TTEQVSRLLRFASENKIPVTARGAGYGYVGGCVPVQGGIALSLARMNRIKEISFEDAI
                     AIVEPGVITGELAEAVRARKLFYPPDPASLRDCTIGGNVATNAGGPRCLKYGVTRHYV
                     IGLEVVLASGEVLRTGGRVHKNKTGFDLIGLFVGSEGLLGVVTEITVRLLPLPPARAT
                     LSAAFATMPQAAAAVQEIFAAGFLPASLEIADHFTLEAARRDRGVTIVPEGNAHLLVD
                     VDGQPESVRSEAAALRTLLESKKPEVLTVATTEEESEKLWALRREFSNSLRATGLTKL
                     NQDIVVPRSRLVDLVEFAGRLQTRHGFPVATFGHAGDGNIHVNIMAANYEDAAVRDRV
                     EKALDELFNQVITWGGVITGEHGIGLAKKRWWPEATSDVARVLHTRLKQALDPLDILN
                     PGKFL"
     gene            complement(8442..9065)
                     /locus_tag="VNW28_00795"
     CDS             complement(8442..9065)
                     /locus_tag="VNW28_00795"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXA08485.1"
                     /translation="MMPPPLHRSLFLAAAVGFGFVSCTELPNDIGVRPHRSVATQTSL
                     ASHFPVLASWNNDPTARGTPKILINLTTQQAFFYRGKILIGKTNISTGRKEFETPPGK
                     YRVIQKDADHISSEYGEVVARSGAVLVRDASVYTPRPKGARFVGAPMPYFMRFTDGYG
                     MHAGFVPHYRASHGCVRMPEEMAQHFFDAAEVGTRVEVIEPPIMVQR"
     gene            complement(9406..9798)
                     /locus_tag="VNW28_00800"
     CDS             complement(9406..9798)
                     /locus_tag="VNW28_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08486.1"
                     /translation="MSCADVWAVGNAGSLSTLVEHWDGTVWNIVGTPDFNLNATNQVL
                     VGVVALSSNSIWTAGQYIVPLLGSAEQTLTENWDGSSWNFVVSPDKKRSNNRLHGITV
                     TPHGTLWSVGTTGVFAQPERTLILRKTP"
BASE COUNT         1886 a         3070 c         3028 g         1962 t
ORIGIN      
        1 aagcacctgc cgatcctgga acaactgaag attgacccga tagccatcgc tcgagacccc
       61 ccgcggatct acccggagcg agaaatgctt gctcgacgcc agccagttta cctcgccgtt
      121 ttggagatct ttgcgtgaca cgccaatgag ttcgaattga ttgtagccga acgtgcggcg
      181 caacgtcgga tcaaggtggc gcatctccac cggcgcgggt cggggccgcg cggaattggt
      241 ggccacgacc agaccactcc agacttgttt gccccgccgc ccaggtttgc cagctctcgc
      301 aggctgcggc gccgccatga gcgtagcgac cccgacgaga aggcagaaaa acgcgcggcg
      361 catcggctat ttcgacgcgt aatcacttgg cagcgccttc aggccgtcca cccagagcac
      421 ggtgacacga tcctgtttcg tttggaaaat cgaaaccgtc gcatatgggc tgacgcccgg
      481 gtcgacccgg gcgttcagga tttgagtgag atactgcgac tggtcgggag cctgttcctg
      541 gcgcatcacg aaaaacgtgc agatggcaaa aaccgcgagc gacgacgcgc ctgtccaaag
      601 caaacggcga atcgtccacc aattctcgcg cgattgcggc gccgggcgcg tggcgggtcg
      661 ctcgctttcc gccgcaatgc gattgcgcag ttgaaggtgg aaaaattcct cgttccccat
      721 ggcccgcggc tgcaactctt ccttgagcaa cgcgccgagt ttgtgcgcct ggtgcttctc
      781 cgcctgggcc gcggcgcgat cgccaagcga ggcctcaaat tccgcacgct ccgcgtcgct
      841 caactggtca tcgacccagg cggtccactt ttcctcaaac gttttcataa acatcgcgca
      901 ataaattctg cagttttttc cgcgcataaa agaggcgcga cataactgtg ccgatcgagc
      961 agcctaacga ctccgcaatc tcatggtatt gcatatcctc gatttccttc atcaaaatca
     1021 cggcgcgatg ctcaggcgag agttgcgcga tggcggcgtc gatccgaacg cggatttcct
     1081 tctgctccat ccgctcgtgc gggagcgcgc cggcgtgggg gacggtgcgg gaagcggggt
     1141 cgatttccgt taactggatc gcgtcatcga attccgcccc ggcaccctgc acctgcttct
     1201 tgcgcagcca gtcgatcgtc acgttcatga cgatgcggta aatccaggta tagaacgacg
     1261 attgcccccg gaatcgcgcg atcgatttcc acgctttcag gaagctgtcc tgggccagat
     1321 cccaggcatc ctgctcgtta tgcaccatgt tgtaaatcat tccgaaaact cggttgcgaa
     1381 agcggctgac gagctggtcg aaggcctcgg tatcgccggc ctgcgcttgc ttcacgaggt
     1441 cgagctccgg gacatcggcc ggcgccgcag tcgtttccgt gtccactttt agacgggtgc
     1501 tggcccgatt cattcaacgg ctagaggctg ccgtagcgac gcagccattc gccgcggaat
     1561 tcgcccatca gcaggatgat gccgtcgcgg acctggaaga gtcgctgttg cagagcggcg
     1621 tgctgcttgc gggtgaggga tttctcgcca atgagctgcg ccccggcgtc catcgctacg
     1681 gtgatcgctt tgagcgatcg cttcaggtag gcgatcgtca tgccgagctc ctcggcttct
     1741 tcgtcgctca aggccgccgc gagcttcgcg ctcgccagtg cggtgtgagt cgccaacttg
     1801 acggcggccg gatcgttttg cagcgccggg ttggcttcga aaagacgatc gatccaaatc
     1861 gtcagggaaa aggcggcgcg gtagaggaca tgctgttcaa aactttcggc gcattcgttg
     1921 atcagattga gggcggaatc ttcatcgccc tcctcatcgt tctccgcgag gagaggatcc
     1981 gcgtcatcat tccaatcctc gccattgtct tcgttgagat gacaccaacc catctcgcgc
     2041 gcgacaattt catcgcgctg cggatcgtcg atgtatttct caagcagctc catgtatttc
     2101 tccgtcttct gatcctgctg ctggagaaac cgttcccact cgtattcatc ccaagcgcgg
     2161 tctgagtcgt gctcggccat gtcttttatc taggccagct tgacaggccg cgcaaaaatt
     2221 ttccgcgtgg gctcaggagt cgggccgacg cacgaatttg cccgttgaac tggttcgcta
     2281 gtaagttgct ccatgcgcac gatcgatgca gatccggatg agcaatttta ccgtgacacg
     2341 gaagccgtcg cgtttccaaa gatcgacgat cgccagctcg cgctgctgga gccgctcgga
     2401 aaacgacgaa cgctgcgacg cggcgagatg gtgttcaagg ccggccagcg cgaccttcca
     2461 ctcacagtga tcctgcgcgg cgagatcgag gttttcgaga aacgcgagga gacggaacag
     2521 attctcggga cgcccggggt tcgtgatttt gtcggggacg ttgcgatgct ccaggggacc
     2581 tcggcgctgg ccagcgcccg agtgaaatcg gacgaggccg agatcttgca ggtcccggcg
     2641 gcggaattgc ggcgggcgtt ggtggagttg cccggcgtgg gcgaaccggt cgtcaatgcc
     2701 ttcatcatgc gccggcagcg cctgatgcgt gaccgggagt ttgccgggct gcgtgtgatc
     2761 gctctccgcg atacgcggga gggacatcag ctgcacgatt tcctcgccaa gaaccacgtt
     2821 ccgcatcgat tgatcgagtt tgaaggcgaa ccgggtcagg cactcgcccg ccgtttcaac
     2881 ctaacgagtc gcgatttgcc ggcgctgatc gcggcgaccg gtgcgccctg gcgccgacca
     2941 tcgttgcgcg aagtcgcgca agttgcgggt ttgcttcgtc ccctcaccca gggggatgaa
     3001 agcgagatcc actgcgacct cgcgatcgtc ggggcggggc cggccggtct ggcggcagcg
     3061 gtttacgcgg cgtcggaagg tctcaaaacg gtggtattgg acggctatgc gcccggcgga
     3121 caagccggct cttcttccct gatcgaaaac ttcttcggct tcccgaccgg gattagcggc
     3181 ggcgatctca cttatcgtgc gcaactccag gcctatcgat tcggcgccaa gttctcgacc
     3241 ccttcgcagg cgctggcgct cagttttcac gatggcgagg agcaccgcgc cagcctgcaa
     3301 atcgagggct gtttcgccac cttgcgctcg aaatgcgtcc tcatctctac cggcgccgat
     3361 tatcgccgac tcgacgcaga aggccgcgaa gagtttgaaa actccggagt ttactacgcg
     3421 gccaccgcgc tcgagggcca gctctgtggc ggcgccaccg tcgtcgtcgc cggcgccggc
     3481 aactccgccg ggcaagccgc gatgtatctc tccgaaggcg cggccaaagt gctcctcgtc
     3541 gtgaggggcg acgatctccg caaaagcatg tcggattatt tgtcgcgccg cgtcgaggcc
     3601 tgccaaaaca tcgaaatcct tcaccgcacc gagattcgca aaatgaccgg cggcaagatg
     3661 ctcgagaaag tcgagctgga gcaactcgaa actggcgaac gtcgaaccgt cgagaccccg
     3721 gctgtgtttt ccatgatcgg cgcaaagcca tgcaccaatt ggctgccacc ggagatcgaa
     3781 cgtgacgaga aagggttcat caagaccggg acggcggtcg ccgcgcaccc ggcctggcag
     3841 aaaagcaagc gccctcccgg tccgttggaa accagcatgc ccggaatatt cgcggcgggc
     3901 gacgtccgct ccggctcagt gaaacgctgc gccgctgcgg tcggcgaagg cgggatggcc
     3961 atcgccggcg tgcaagcggc cctcgcttct cctgcctaac gaacgctagg cggtcaccgg
     4021 ctgacgctcc ctccgcttcg tttggaggcg caactgcccg caggcggcgt cgatgtcgtg
     4081 gcccttctcg cggcgcaacg tcgcgaccgc gccgtgctcg cgcaggatac ggaggaaact
     4141 ctcctggcga ttgcgcgacg ggcgcgtcca ggcgaggcct tcgactgtgt tgtagggaat
     4201 gaggttgatt ttcgccgaga gacacgcagc gtgtcgggcc agggcgtggg cctgctcatc
     4261 agtgtcgttc accccggcaa tcaggatgta ttcgaacatg agccgcttct tctgcgcgac
     4321 atagtcggcg cacgccgcca ggagggtcgc aagattgtat ttgcgattca ccggcatgat
     4381 cttgctgcgc acttcgtcgg tcgcgccgtg caatgaaatc gcaaggcgaa actgggtcgg
     4441 ttcggtggcc aacttccgaa tctgcggtgc cagaccgctg gtcgagatgg tgatgtgacg
     4501 ggctccgatc tccagacccc acggcgcatt aatgatccgg atcgcccgga gcaggttcgt
     4561 gaggttagcg agcggctcgc ccatgcccat gaagacgata ttgtcgatcg tctcgccgct
     4621 cgccgcttcg atcgcgagga gctgatcgac gatctcgtcc ggctcgagat tgcgggtaaa
     4681 tccatccagg ccgctcgcgc aaaacctgca gccgtaggcg cagccgacct gggtcgaaac
     4741 gcaggcggtg cggcgatccg aagctgcgcc atagagagcg ggcgacgccg ggatgagcac
     4801 cgactcgatc aggttgccgt ccgcgaggcg gaagagatat tttcgtgtcg tgtccttcga
     4861 accaagcatc tgcacgattt ccggcctgac gaaactgaat tcctccgcca gccgcgcccg
     4921 ggtggcagcc ggcaggtcgg tcatcgcggc gaaggaatca acccgcttgc ggtaaagcca
     4981 gtctgcgacc tgcttcgcgc gataggccgg ctcgccccgg gccgcaaaat gcggagtcag
     5041 ctcctcgggg gtgagcgatt tgatcgcggg cttcgccacg agcgaatcta acggcgcggc
     5101 cacggttgtc atttgcccgc caccaggatg ccgtccggct gttctttcgt gtcgggcaag
     5161 tcgatctcga tcttgtaacc gctggcgcgc agcagctcgc gacaatcgtc atgcagcgtc
     5221 aagccgcgcg cgcggccgtg cgtgccgatc aaaaaggtct tcaccgcgcc cgccttcatg
     5281 gcttccgtcc cgcctttcag cacctcaact tccaagcctt gcacgtccat ctgcaggaga
     5341 tcgatcggcc gcccgatccg ccgcacgaag tcgtcgagcg tgatggtcgc gatggtcacc
     5401 gtccggttgc gcggccggcg cagcccgacg ccctccaaga aacgcttcca gcgcgcactt
     5461 ggcgaaaggg tcctttcccc ggccaccgcc gcaagccggc tgctgtaacc gcgatccatg
     5521 agggtctcgc ttccggcgga tgaattgagg ccttccgcgt gcagggtaag gtcatcattc
     5581 gttaggccat tcagggtcat attttcccgg agaaaccggc gatggcgctc caacggttca
     5641 accccgtgaa tcgttaggcg cggggccagt gtccgggcca ggatgaggta gtaaccgatc
     5701 gccgcgccga tatcgacaaa acagcccccg ggaggaagtc gccggatcag gtcaacaaag
     5761 atttcttctt cttccgcttc gtggacgttg cgtttctcgt agcgtgcccg ctgctcatcg
     5821 aggaggtgat cctcataaac gcggacgccg ttgtcgaaga tgtggacggc gcgcgccatg
     5881 gcccgaacag tctatcacaa gcgaggcagc cgttcgcccg gaggtggcgc tggtccaaga
     5941 gggggccgtg gaaggaaggc ttgcctaacg aacgatcgtc gcgctctttc ccttcgcctt
     6001 ggcgagctcc tgctgcgcct tgcgctggtt gataacgaag gtgttgttgt cgccgagctt
     6061 cgcccagggg ccgggcggca cctcgttgaa gtcgacaaat ttccagtcgg tcacatcggt
     6121 gtcctggagg ccgagataat cgcgcaccgc cggagaaacg tcgagtccgg caccgcggtt
     6181 gagattcgac ttcggccgct cgttgccgaa aacatattcc cagtgatcgg tgcgaaacgg
     6241 gccggcatct tcccactgcg cgtagaccgt gcgatcacct ttgtggatcg cgacccagcg
     6301 ccctttgcaa acggaaacgc ccggtccctg atacgcttcg ttgaaccagg gaatgaccct
     6361 cgctgcctcc ggccgatgac cttcgcgtga cttgtcgttg taaggcagcg cacaataaaa
     6421 cggattttgc cgcggagtga acttcaccgg aatgtaattc tgccggcgcg ccggattcgg
     6481 atcgtcatag ccgccgtaat ctttcgccca atccgcgtcc caactactgg cgtgattcgg
     6541 caccgggttg ttcgcggttg gattttcgcc aatccagaag acggtcgtga caatgtcctt
     6601 tttccagggg aaacgctcgg cgccttttct ggtcggaata cctgcgacgc caggcggggt
     6661 gtagccgatc gggtccactt tcagcaggac gtcctcgttc aggcggttct gctttttgct
     6721 tagtttaaat ttcgccgtga cgggctgggc cggcgccgaa ggcgcgaaaa cggccgaggc
     6781 cgaccccaac aaaacggcga tggtatgagt gagtgttcct ggtttcatca aagcaaagct
     6841 ccagcaccgc cggagataaa gcgcaccaat taagactcgg tctgaagacc ggaaatgcgc
     6901 gtgggttgaa ctaacggacg atagcacgac acccgccggg atcaactgtc ttagccaata
     6961 aattttccga ctgtcgactc ttccctctgc gaaacgcgag ggtcgctgcg gccgcgacgt
     7021 gcacgctcgg cctcccacag ggaggcgtct acaggggcga gcgccccaag ggagcctcta
     7081 caggaatttc ccgggattca gaatatcgag cgggtcgagc gcctgcttga ggcgtgtatg
     7141 cagcacacgg gccacgtcgc tggtcgcttc gggccaccag cgcttcttgg cgagaccgat
     7201 gccgtgctca ccggtaatca ccccgcccca ggtgatgact tggttaaaca gctcgtcgag
     7261 agccttttcg acacggtcac gtaccgccgc atcttcgtag ttggcggcca tgatattgac
     7321 gtgaatgttg ccgtcaccgg catggccaaa agtggcgacc ggaaagccgt gccgcgtctg
     7381 caggcggccc gcaaactcta ccagatcgac gagccggctg cgcggcacga cgatgtcctg
     7441 gttgagtttc gtcaagccag tggcgcggag ggaattgctg aattcgcgtc gcagcgccca
     7501 cagtttctcc gattcttcct cggtggtggc gacggtgagc acttctggct ttttgctttc
     7561 aagcaaggtc cgcaacgccg ccgcttcact tcggacgctc tccggctggc catccacatc
     7621 gacgagcaga tgcgcgttgc cctccggcac gatcgtcacg ccgcgatcgc ggcgcgcagc
     7681 ttcgagggtg aagtgatcgg caatttcgag cgacgccggc aaaaatcccg cggcgaagat
     7741 ctcctgcact gcggcggcgg cttggggcat ggtggcaaaa gcggccgaga gcgtggcgcg
     7801 cgccggcggc aacggtagca ggcgaacggt gatctcggtc accacaccta acaaaccttc
     7861 ggagccgacg aagagtccga tcaaatcgaa gccggtcttg tttttgtgca cgcgtccccc
     7921 ggtgcggagc acttcaccac tcgcgaggac cacctcgagg ccgatgacgt aatgccgggt
     7981 gacgccgtac ttcaggcagc gtgggccgcc ggcattggtg gcgacatttc cgccgatggt
     8041 gcaatcccgc aggctcgcgg ggtcgggcgg ataaaagagc ttccgggcac gaacagcctc
     8101 tgccagttcg cccgtgatca cgcccggttc cacgatggcg attgcgtcct cgaaactaat
     8161 ttccttgatc cgattcatcc gcgcgagcga gagcgcgatc ccgccctgga ccggcacgca
     8221 accgcccaca tagccatagc cggcgccgcg ggccgtgacc gggatcttgt tttccgaggc
     8281 gaaacgcaat agccggctta cttgttcggt cgtgcgcgcg aaaacgacaa cctccggctc
     8341 gtgatgggca aaccacttgt cgccgccatg gacaattcgc gttgcttcgt cctccgcaac
     8401 aatttctccg ccgaggagcg cacggagttt ggcggcgagg ctcatcgttg caccataatc
     8461 ggtggctcaa tcacttccac ccgcgtgccc acctcggccg cgtcgaagaa atgttgcgcc
     8521 atctcttccg gcatgcgaac gcagccgtgc gaggcgcggt agtgcgggac gaagccggca
     8581 tgcattccat aaccgtcagt gaagcgcatg aaatacggca tcggcgcgcc gacaaaccgg
     8641 gcgcctttcg ggcgcggagt atacacgctg gcgtcgcgga ccaggaccgc gccggagcgc
     8701 gcgacaactt caccgtactc actcgaaatg tgatccgcgt ccttttggat aacacggtac
     8761 ttgccaggcg gtgtctcaaa ctctttgcgg ccggtggaga tgttggtctt tccgatcaag
     8821 atctttcccc gataaaaaaa agcctgctgc gtagtgagat tgatgagaat tttcggcgtg
     8881 cccctggcag tgggatcgtt attccaggag gcaaggaccg gaaagtgcga agcaagcgag
     8941 gtctgggtcg cgaccgaacg gtgcggacga acgccgatgt cgttaggcaa ttcggtgcaa
     9001 ctgacgaaac cgaaaccgac cgccgccgcg aggaagagag aacggtgcag cgggggcggc
     9061 atcatgatct cgtgattgta acgtcgcgcc gctcctttca caacgagtgc tgcgccaccc
     9121 ggaggaacag ttatcgccaa tctgaatgca caggacaccg actctttcag tcgccgcccg
     9181 ttccctcctc acggttgttg cggtgatgtt ctgtctccgt gttccaagaa cccggccttt
     9241 ggtctctctg gtcgcaaact cgctcctcgt ccgccgtgac tgagtcacag gctcgggcgc
     9301 cttttctatc catgcctcgc accaagctga tcgaggatcg cgagaactac cggtacgccg
     9361 aataccgccg cgacggcggc gtcgaatgcg gccgtcgcaa attcgttagg gggttttccg
     9421 cagaattaac gtccgctcgg gttgggcgaa cactcccgtc gtgccgacgg accacagtgt
     9481 gccatgcggc gtgacggtga tgccgtgcag gcggttatta gagcgctttt tatcggggct
     9541 tacgacgaag ttccaactcg atccgtccca gttctctgtt aaagtctgct cggcgctgcc
     9601 caagaggggc acaatatact gaccggcggt ccaaatactg ttgctcgata gagcgacgac
     9661 gccgacgagg acctgattgg tagcgttgag gttgaaatcc ggcgtgccga caatattcca
     9721 gaccgtgcca tcccagtgtt caacgagtgt gcttaaactg cccgcgttac cgaccgccca
     9781 gacatcggcg caagacattg cctcacaaca agctcttcgt aacgggtttg ttacgttcag
     9841 aacaacctcc aggggacgaa cagcctaaat cagtaatcca cgagacaggc ccgcgaaaag
     9901 agcccggaaa ctctgggtcc actctcctgc actacgcctg ctgctc
//
LOCUS       DATMNS010000020         5864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_31665, whole genome shotgun sequence.
ACCESSION   DATMNS010000020 DATMNS010000000
VERSION     DATMNS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31665"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VNW28_00805"
     CDS             <1..642
                     /locus_tag="VNW28_00805"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXA08487.1"
                     /translation="LAKAKKATFLLASTSECYGDPLVHPQKEDYWGNVNPIGPRGVYD
                     EAKRFAEAITMAYHRYHSMDTKIVRIFNTYGPRMRLRDGRVVPAFIGQALAGQPLTIF
                     GDGSQTRSFCYVSDLIDGIFRLSQSDFHEPVNIGNPREMTIKQFAEEIIRITGTKSGI
                     EYKPLPVDDPKVRQPDIARAKKILGWEPKVEFEEGIVRTIDYFRQWLERARPR"
     gene            882..1322
                     /locus_tag="VNW28_00810"
     CDS             882..1322
                     /locus_tag="VNW28_00810"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HXA08488.1"
                     /translation="MTGKNLTQEPPRSPKTLVGGYAILGRTLDKCRALLAGNIGEYHF
                     DCPLDNMLFGWKRIMGDNFKAKVAKGASDEEMVQWVDTHGRPKTDDEKKAWVQEVKKA
                     SLYNDPAKRDIFIAETKKLGLDPATTTLFDWLDADDKASYPKAG"
     gene            1417..1983
                     /locus_tag="VNW28_00815"
     CDS             1417..1983
                     /locus_tag="VNW28_00815"
                     /inference="COORDINATES: protein motif:HMM:NF042424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1102 family pilus-like protein"
                     /protein_id="HXA08489.1"
                     /translation="MHRIIAVAILVCCLGGSVSAQNRRSAIQLIKISRDLILAPDFNY
                     TGAEKLHESHERWLKVEVQFAAAPEFTDEVTFKYYILIGGKTLTGEVTHVNVLAGREL
                     YSCMYVPPHALAYVLRNRPPNTTLVENVAVQVLQKGEVKDELSMARARPQWFTSLPAL
                     PGFVLNKSETPFAPLLWGYYEQIKPVRR"
     gene            1993..3744
                     /gene="pilM"
                     /locus_tag="VNW28_00820"
     CDS             1993..3744
                     /gene="pilM"
                     /locus_tag="VNW28_00820"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HXA08490.1"
                     /translation="MSASPRILSLDLGSQTLRLAEFQAGPNGGLILTGYRRREILADP
                     AAETDRTGQISEVLSAMMRELGIKGGPVDYAVPGQSVFARFVKLPLVDQDKIERIITF
                     EAQQNVPFPIEEVVWDYQLVGSSDVDKIEVVLVAIKADLLEGMNAAVEAAGLQTMRVD
                     VAPMALYNAFRFNYSELTGSSLVIYIGARTTNLLFIEPGKVFTRSIPLGGSSITSAIA
                     KEFNEPFGAAELRKRHDGFVSLGGPEAAASDPDVARVAKLVRSTMTRLHAEIARSISF
                     YRTQQQGNQPERVFLCGGAASTPYVCEFFQEKLQSPVEFFNPLRNVALGESVAFEEVA
                     GSVHLLGEVVGLALRSTMTCPMELNLRATNVVRRQRLAQRRPFLVLAGLCFVLGLLGW
                     GTYFLRVAQVETRVAATLQTDVDQMRLFERQLDKLRKETAALDAAAKPLLNAINDRGV
                     WLRIIDDLNARLPKENIWITELAPTSEGRIVGETAMGLTASGDRASAALARGRAAGAK
                     SVPMIDGVFVRGLYLSNPKQQEIVVDYFRNLLSSTVFEVDSNKQSEVIKPSTPTNTEW
                     AYPYELRLRLRRPFALP"
     gene            3741..4679
                     /locus_tag="VNW28_00825"
     CDS             3741..4679
                     /locus_tag="VNW28_00825"
                     /inference="COORDINATES: protein motif:HMM:NF038287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1100 family pilus-like protein"
                     /protein_id="HXA08491.1"
                     /translation="MNWFRQNRFLGSFLAALALGTLLCGYFLRHEKGAADEGRDRLEA
                     TINELTRLRRSSPFPNEENLRKTKAQTDSYRASLRALEKELKTRMFPKRPLQPNEFQA
                     QLRQTTLALGDRARASKVQLPENFNLGFDEYATSLPNGLAAPRLGRQLKAIEWLANTI
                     VDAQVDVLNGLARSTLPEEKVAPTPTTAGVKLPDRTPKAVEESAGIVDSTSVDLAFTG
                     SPAAARRVFNQVTSAKEQFYIIRALRVKNQADKGPKRDGLSATAPRAAVVRQSSAGSA
                     ARTPEQGITFIVGTEHLTINAKIEIVQFNFPEKETR"
     gene            4683..5636
                     /locus_tag="VNW28_00830"
     CDS             4683..5636
                     /locus_tag="VNW28_00830"
                     /inference="COORDINATES: protein motif:HMM:NF042425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1099 family pilus-like system protein"
                     /protein_id="HXA08492.1"
                     /translation="MSWIRANYDRAALLVTAFFLVTCASFIFVSASGFGEKFDALRNV
                     PPSNRQIPSGQGPEIVEAMQKLRTPSQWISNGRSGLFVPEKHFVGPDGRPVTLQNTLL
                     HPPVPNEWLEELGLPITDADVLTQDPDHDGFANLDEWEGHANPTDKESHPPYTSKLKL
                     RSFAQEAFPLIFASSVGDTYAINSVDRQMPTQFLQIGELVAGTKYKLADYAEKYDTNK
                     YGTAIDVSELTLEQVDSHDRVTLVKERRTTSPESVANFLYTWGGAEQSFAVRKDQEFS
                     LKPLEQIKYKLLDVQPDKAVIIDTQKPNEKIEIGPVPRPNP"
BASE COUNT         1240 a         1766 c         1742 g         1116 t
ORIGIN      
        1 ctggcgaagg cgaagaaggc gactttcctc ctcgcttcga cttccgagtg ctacggcgac
       61 ccgctcgtcc atccgcagaa ggaagattat tggggaaacg tgaacccgat cggcccgcgc
      121 ggcgtttacg atgaggcgaa acgtttcgcg gaggcgatca cgatggccta ccaccgctat
      181 cacagcatgg acacgaagat cgtccgcatc tttaacacct acggcccgcg gatgcggctg
      241 cgcgacggcc gcgtcgtgcc ggccttcatt ggccaggcgc tcgccggcca gccgctcacc
      301 attttcggcg acggctcgca gacccgatcg ttttgttatg tctccgattt gatcgacggg
      361 atctttcggc tttcgcaatc cgatttccac gagccggtca acatcggcaa cccgcgcgag
      421 atgacgatca aacaattcgc cgaggagatc atccgcatca cggggacgaa atcagggatc
      481 gaatacaagc cgctgccggt ggacgacccg aaggtgcgcc agcccgacat tgcgcgggcg
      541 aaaaagatcc tcggctggga accgaaagtg gaattcgagg aagggatcgt gcgcacgatc
      601 gattacttcc ggcaatggct cgagcgcgcg aggccgcgct gaggaaacga caatcggggc
      661 cgccttggtg cgacagccag gcgagacctg ttcggtcgga aatgacatca cggtggacag
      721 atgattacga aacgaaattt gcaattttcg tcttgactgt ttcctccgcg ctctcgtaga
      781 aacggatcgt tctgggggga acatggtcgg gcgtggtcga tttcatcatc gctgcgcccg
      841 gcctactttc cggataatat tcgcaccgga atgaagcaaa agtgacgggc aagaatctaa
      901 cccaggaacc cccgcgcagc ccgaaaactc tcgtcggcgg ctacgcgatt ctcggccgta
      961 cgctcgacaa atgccgggct ctgctggcgg gcaatatcgg cgagtatcat ttcgattgtc
     1021 cgctcgataa catgctcttc ggttggaaac ggattatggg cgacaacttc aaggcgaagg
     1081 tcgccaaagg cgcgagcgac gaggagatgg tgcaatgggt cgacacccac ggccgtccga
     1141 aaactgacga cgagaaaaag gcgtgggtgc aagaagtgaa aaaggcttcg ctctacaacg
     1201 atcctgcgaa acgcgatatt ttcatcgcgg agaccaaaaa gctggggctc gatccggcga
     1261 cgacaacgct cttcgactgg ctcgacgccg acgacaaggc aagctatccc aaagcggggt
     1321 aggctggtcc ctgcgcagac gattagtcgg ggcacacagc ccctcgcaca ttcctttctc
     1381 gctttctgcg cgcgcttcgt ctgaaatccg aacttcatgc atcggataat tgctgtagcg
     1441 attctggttt gctgcctcgg cggctctgtt tccgcacaga accgcaggag cgctattcaa
     1501 ctcatcaaga tcagtcgcga cctgatcctg gcgccggact tcaactacac tggcgccgaa
     1561 aagctccacg aatcgcatga gcgctggctc aaggtagagg tacagttcgc ggccgcgccg
     1621 gaattcaccg acgaagtgac tttcaagtac tacatcctga tcggcgggaa aaccctcacc
     1681 ggcgaggtga cgcacgtcaa cgtgctcgcg ggtcgggaac tttactcctg catgtatgtg
     1741 ccgccgcatg ccctcgctta tgtcctccgc aatcgcccgc cgaatacgac cttggtggag
     1801 aatgttgccg tgcaggtctt gcaaaaagga gaagtgaaag acgagttgag tatggcccgg
     1861 gcacggccgc aatggttcac gtcgctgccg gccttgcccg gcttcgtcct gaacaagagc
     1921 gagacgccgt tcgcgccgct cctttggggt tactacgagc agattaaacc ggtccgccgt
     1981 tgattgccat ccatgtcagc ttccccccgc attctcagtc tggacctcgg ttcgcaaacc
     2041 ctaagattgg ccgagtttca ggctgggcct aacggcggcc tcatcctcac cggctaccgg
     2101 cggcgcgaga ttttggccga tcccgctgcg gaaacggacc gcaccgggca gatcagcgaa
     2161 gtgttgtccg ccatgatgcg cgaactcggg atcaagggcg gcccggtcga ttacgcggtc
     2221 ccggggcaat cggtcttcgc ccgttttgtg aaattgccct tggtcgatca ggataaaatc
     2281 gaacgcatca tcacctttga ggcgcagcag aacgtgcctt ttccgatcga ggaagtggtc
     2341 tgggattatc agctcgtcgg gagcagcgac gtggacaaaa tcgaggtcgt gttggtcgcg
     2401 atcaaggccg acttgctcga ggggatgaac gccgcggtcg aggcggccgg cttgcagacc
     2461 atgcgggtgg acgtagcgcc gatggcgctc tacaacgcgt ttcgtttcaa ctacagcgag
     2521 ctgaccggct cctcgctcgt catctacatc ggcgcgcgca cgaccaacct gcttttcatc
     2581 gagccgggaa aagtcttcac ccgcagcatc ccgctgggcg gcagttcgat cacctcggcg
     2641 attgcgaagg aattcaacga gcccttcggc gcggccgagc tgcgcaagag gcacgacgga
     2701 ttcgttagtc tcggcggccc ggaagcggcc gcctccgacc cggatgtcgc acgcgttgcg
     2761 aagctggtcc gcagcacgat gacgcggctg cacgccgaga tcgcgcgctc gattagtttt
     2821 tatcgaacgc agcagcaggg gaaccagccc gagcgcgtct tcctctgcgg cggcgccgcg
     2881 agcacgcctt atgtgtgcga gtttttccag gagaaacttc agagcccggt cgagttcttc
     2941 aatccgctgc gcaatgtcgc cctcggggaa tcggtcgcgt tcgaggaggt cgcaggctcc
     3001 gtccatttgt taggtgaagt ggtcgggctt gcgcttcgca gcacgatgac gtgtccgatg
     3061 gagttgaacc tgcgagccac caatgtcgtg cgccggcagc ggctcgcgca gcgccggccg
     3121 tttttggtcc tggccggcct ctgctttgtc cttggcctgc tcggctgggg tacttatttc
     3181 ctgcgcgtgg cgcaggtcga aacgcgtgtg gccgccacgc tgcaaaccga cgtcgatcag
     3241 atgcgcttgt ttgagaggca actggacaaa ttgcgcaaag agaccgccgc gctcgatgcg
     3301 gcggccaagc ccttgctcaa cgcgatcaac gatcgcggcg tctggctccg catcatcgac
     3361 gatctcaatg cgcgcctgcc gaaagaaaac atctggatca ccgaactggc gccgacctcg
     3421 gaaggccgga tcgtgggcga gaccgcgatg gggcttaccg cctccggcga cagagcgagc
     3481 gcagcgctcg cccgcggtcg agccgccggc gcgaagagcg ttccgatgat cgacggcgtg
     3541 tttgtccggg ggctttatct ttcgaacccg aaacagcagg aaatcgtggt cgattatttc
     3601 cgaaatcttc tgagctcgac cgtatttgaa gtggattcga acaagcaatc cgaagtgatc
     3661 aaaccgagca cgccgacaaa cacggagtgg gcttatccct acgagttgcg gctcaggttg
     3721 cgccggccct tcgccctgcc atgaactggt ttcgccagaa caggtttctc ggcagcttcc
     3781 tcgccgcgct cgcactgggg acgctcctct gcggctattt tctcaggcac gaaaagggcg
     3841 ccgccgacga gggacgggac cgactcgagg cgacgatcaa cgagctgact cggctccggc
     3901 gaagcagccc cttcccgaac gaagagaatt tgcggaagac aaaagcgcag accgacagct
     3961 accgcgcttc gctccgcgcc ttggagaagg agctgaaaac ccgcatgttt ccgaagcgac
     4021 cgcttcagcc taacgaattc caggcgcaac tgcggcagac gactctcgcg ctcggcgacc
     4081 gggcgcgcgc gagcaaagtg cagttaccgg agaatttcaa tctcggcttc gatgagtatg
     4141 cgacttcgct ccccaacggc ctcgccgccc cgcgcctcgg gcgtcaacta aaagcgatcg
     4201 aatggcttgc caacacgatc gtcgatgccc aggtcgatgt gctcaacggc ctggcgcgca
     4261 gcacgctgcc cgaagaaaaa gtagcgccga cgccgaccac ggcgggggtg aagcttccag
     4321 acaggacgcc aaaagccgtg gaagaaagcg ccgggatcgt cgactcgact tccgtcgatc
     4381 tcgcgtttac cggcagcccg gcggccgcgc gcagggtgtt caaccaggtt acgagcgcga
     4441 aggaacagtt ctacatcatc cgcgcgctgc gggtgaagaa ccaggccgac aaaggaccaa
     4501 agcgcgacgg cctgtcggca acggcgccgc gtgccgcggt ggttcgccaa agttccgccg
     4561 gcagcgccgc ccggacgccg gagcagggaa tcacctttat cgtcggaact gagcatctta
     4621 ccatcaacgc gaaaatcgag atcgtgcagt tcaactttcc ggaaaaggag actcgttagg
     4681 gcatgagctg gatccgggcc aactatgatc gcgcggccct cctcgtcacg gcctttttcc
     4741 tcgttacctg cgccagcttt atttttgtga gcgcttccgg tttcggcgaa aaattcgacg
     4801 ccctccggaa tgttccgccg tcgaacaggc agatcccgtc gggccagggg ccggagattg
     4861 ttgaggcgat gcaaaaattg cggacgccgt cgcagtggat ttcgaacggg cgctccggtc
     4921 tcttcgtccc ggaaaagcat tttgtcgggc cggatggacg gccggtcacc ctgcaaaaca
     4981 cccttctcca tcctcctgtg cctaacgaat ggttggagga actcgggctc cccatcaccg
     5041 acgctgatgt gctcacccag gatccggatc acgacggctt cgccaatctc gacgagtggg
     5101 agggccacgc gaatccgacc gacaaggaat cgcatccgcc ctatacctcg aagttgaagc
     5161 tgaggtcctt cgcgcaggag gcattcccgc tgattttcgc gtcctcggtc ggcgacacct
     5221 acgcgatcaa cagcgtcgat cggcagatgc cgacacaatt cctgcagatc ggcgagctgg
     5281 tcgcaggcac gaaatacaag ctcgccgact acgccgagaa atacgatacc aacaagtatg
     5341 ggacggcgat cgacgtctcg gagttgactc ttgaacaggt ggattcgcac gatcgcgtga
     5401 cgctggtgaa agagaggcgc accacctcgc ccgagtcggt cgcaaacttt ctttacacct
     5461 ggggcggagc ggaacagtcc ttcgcagtca gaaaagatca ggaattttcc ttaaaaccgt
     5521 tggagcagat caaatacaaa ctgctggacg ttcagcccga caaagccgtc ataatcgaca
     5581 cgcaaaagcc taacgagaag attgagatcg gcccggtgcc gcgcccgaat ccttgaattc
     5641 tgaaatttgc cgtaagcagg tcaagttttc tgtggcgcgg aggaaaatga caaatgacga
     5701 aggaatgacg aagcactaat gacgaatgcg gcggccagca cgctcccacc ttcgggcgtt
     5761 gtcatttgga attccttcga ccttggtcat tcggaattcg tcatttttcc ggcgttattt
     5821 ccttgctgga gtcaccggct ccgaatacaa ccaccacacc tacc
//
LOCUS       DATMNS010000021         6747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3596346, whole genome shotgun sequence.
ACCESSION   DATMNS010000021 DATMNS010000000
VERSION     DATMNS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6747
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3596346"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..450
                     /locus_tag="VNW28_00835"
     CDS             <1..450
                     /locus_tag="VNW28_00835"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXA08493.1"
                     /translation="LIKTMTDDELMQAAMAQARKSRSEGGIPIGSALARHGELLAVGH
                     NKRVQESDPVTHAEIDCLRQAGRIGTFRDTVLYSTLMPCYLCAGAVVQFGIPKVIVGE
                     SRTFPGAPDFMRSHGVEVIDLDLPECVEMMTQFISAHPQLWNEDIGR"
     gene            complement(605..1777)
                     /locus_tag="VNW28_00840"
     CDS             complement(605..1777)
                     /locus_tag="VNW28_00840"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HXA08494.1"
                     /translation="MNTKILACLLLFALGAQAREPQTTPPPTTNMRARALGIPFGGTP
                     GKLNAITDVAGVEVGFTTLISGEGKLEVGKGPVRTGVTAIIPRGHDSLNDPVYAGTFG
                     LNGNGEMTGTAWVEESGFLEGPVIITNTHSVGVARDAVIAWRLAHGAADKTGYWWSLP
                     VVAETWDGWLNDINGFHVKPEDVAHALDTAKGGPVEEGSVGGGTGMICYEFKGGTGTA
                     SRALDPKTGGYTVGALVQANCGRRPQLMIAGVPVGQEIPGSVYPKETGSIIIVVATDA
                     PLLPGQLKRLARRASLGLARTGSVSGNGSGDLFLAFSTANRGAANPEQPTQTVQTVPN
                     DRLDPIFEATVQSVEEAIVNALVDNHTMTGSDHHKVEALPHDRLRELLKKYNRLSD"
     gene            complement(1781..2881)
                     /locus_tag="VNW28_00845"
     CDS             complement(1781..2881)
                     /locus_tag="VNW28_00845"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HXA08495.1"
                     /translation="MESTIQRDESDDQLSVFFLAEGEQSGASVMGRLTHFLGQAQRSL
                     DLAVYDMRLDDELRLQLLQALEERAAAGVQIRICYDGDKPETPNVAGGQDPAPPGTGA
                     FVQGLGFPWRRIGGVKLMHDKFIVRDGAAVWTGSLNLTNDAFTLMENNVIELDSPVLA
                     AFYAEDFEQIWEKGNFENTGHLKTTSATLRFAGEPVTVRVMFSPGCGLEIDAEVARRV
                     RAAQQRVRLCSLLINSGTLISALDHLLGAGRVKVDGIFDRTQMEGVFRQWQTVPSNQW
                     KIPAVRQIIARAHLVGKNSTPYTPTGRHDFMHNKVLIIDDTVITGSYNFSRSAQFNAE
                     NILFLESAALAQRYSVYIDHLLLKYGVTQPSL"
     gene            3091..4785
                     /gene="dnaX"
                     /locus_tag="VNW28_00850"
     CDS             3091..4785
                     /gene="dnaX"
                     /locus_tag="VNW28_00850"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HXA08496.1"
                     /translation="MSYQVFARKYRPQTFDDLVGQAHVTQTLKNAVEQNRLAHAYLFV
                     GPRGIGKTSTARILAKALNCIHGPTITPCGVCDSCKEITAGNSLDVLEIDGASNNKVE
                     EVRTLTENVRYAPVKGKYKIYVIDEVHMLTPSAFNALLKTLEEPPEHVKFIFATTEPQ
                     KVLPTILSRCQRFDLHRIPANLIAQHLQFIAKKEKIKLDSAAAHAIARGADGGLRDAE
                     SMLDQLVAFCGETIAESDVLNVFGFTSEQTIARFTEKILRGETADALGLLDEQSAAGK
                     EMMKLMSDLIAHLRDLLVFKVKPDALAEEAAPELQSSLGEEAALIETDRLLELIEQFA
                     EAEGRMKWAPNKKLHFEVAVIKAIQTLSQVTLTEVIENLTALRGGGASIPSVPKPPVA
                     EKKAAPKAGQPTVLAATPDPTPGGSPHAGASHIPAATPPVEMAAIWPKAVQLASARRP
                     LIKSWIDSASLLGTEGRTVLLGFAPDQKTVMESLSRPSNRSFLEALLKELTGSDWTVK
                     LSLAEGLPPTPPPDETRPELEPVRAGPNDSLESFRNDPLIKEALEIFKGEIKSVTT"
     gene            4789..5103
                     /locus_tag="VNW28_00855"
     CDS             4789..5103
                     /locus_tag="VNW28_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXA08497.1"
                     /translation="MNLNKLMKQAQKMQEQMARTQAELEEKTVEVSAAGGKVTVVANG
                     AGDVLSIKIAKEIVDPEDVEILEDLILSGVQQAIAQGKELASSEMTKITGGMPGLSGL
                     GL"
     gene            complement(5347..>6747)
                     /locus_tag="VNW28_00860"
     CDS             complement(5347..>6747)
                     /locus_tag="VNW28_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08498.1"
                     /translation="YTTEMLLDAGQQIIDATMAAFPNQYVTLAIGGDGYTNTGPNLDP
                     TATYAAATTISLSRGKWPGRLIVQVNSLSTFNPVAPGPDDSVWNVLWNSQPDVGAQML
                     AGVYGDTTYRVNNGIPGDPATILTTSVDAGVSYGVNYIEIYEVDVRNLPSVITYARTK
                     LQATPTPTPTPTSTPTPTPTSTPSPTPTPTLTPTPTPSPTPTPSPTPIPFNLDGQADF
                     AGYLQDADGLTLYAAVRGSTLYVATSSPGGDGEPNDNFIFVTDELLPAASALAPWAKT
                     GLIAIDPSKPFVGGESTSDFCGWFNAPLSSLAVKSATSSGQMEATIDLAAAFGAVPDT
                     VYVAAAAYATADGGSLAGQCPLGNGDGNIDPDEFLALSIAALKDENADGIYDRLDPIL
                     GFGITQITRTGEVTTISWASVPGRTYQTESCDQLGGTWAPLNSPITAGPGQLTTSTTD
                     STDLPSRFYRVQLVNP"
BASE COUNT         1387 a         1928 c         2128 g         1304 t
ORIGIN      
        1 ctaatcaaaa ccatgaccga cgacgagttg atgcaggccg ccatggcgca ggcgcgcaag
       61 agccggagcg aaggcggcat tccaatcggc tctgctctgg cgcgacacgg tgaactactc
      121 gccgtcggtc acaataagcg agtgcaagag agcgacccgg taacgcacgc cgagatcgat
      181 tgcctgcgtc aggctgggcg gatcggaacc ttccgcgaca ccgttctcta ttcgacgctt
      241 atgccctgct atctctgtgc tggcgcggtg gtgcagttcg ggatcccgaa ggtaatcgtg
      301 ggcgaaagcc ggactttccc cggtgcgccg gacttcatgc gcagtcacgg agtcgaagtg
      361 atcgatctcg atctaccgga gtgcgtggaa atgatgacgc aatttatcag cgcgcacccg
      421 caactttgga acgaagatat cggtcgttag gggaatgcgt gtgccccgcc cgatggaacc
      481 gcggattacg cggatttaac ggattggaag aagagagaaa agagtgcgca ggctctttct
      541 tgtcttttta tccgcgttct ccgcgccatc cgcggttcaa acgatgatcc gcttcgcgaa
      601 aggttcaatc gctcagtcga ttgtatttct tcaagagctc gcgcaagcga tcgtgaggca
      661 acgcttcaac cttgtgatgg tcgctgccgg tcatggtatg gttgtcgacc agggcgttaa
      721 cgattgcttc ctcgacggac tggaccgtgg cttcgaagat cggatcgagg cgatcgttag
      781 gcacggtttg caccgtttgg gttggctgct cgggattggc cgcgccgcgg ttggccgtcg
      841 agaaggcgag aaacaaatcg ccggagccat tgccggagac gctgccggtg cgggccaggc
      901 ccagactggc gcggcgggcg agacgtttca actgtcccgg taagagcggc gcatcggtcg
      961 cgaccacgat gatgatcgag ccggtttctt ttggataaac cgagccggga atttcctggc
     1021 cgacgggcac gcctgcgatc atcaactgcg ggcgccgtcc gcaattcgcc tggacgagcg
     1081 cgccgacggt gtagccgcca gttttcgggt cgagcgcccg cgaggccgtg ccggtgccgc
     1141 ctttgaactc ataacaaatc atcccggtcc cgccaccgac gctgccttcc tcgaccggcc
     1201 cgccctttgc cgtgtcgagg gcatgcgcga cgtcctccgg tttcacatga aagccgttga
     1261 tgtcgttcaa ccatccgtcc caggtctcgg cgaccaccgg caggctccac cagtaaccgg
     1321 tcttatccgc cgcgccgtgc gcgaggcgcc acgcgatgac cgcgtcgcgc gccacgccca
     1381 cgctatgggt gttggtaata atgaccggtc cctcgagaaa gccgctctct tccacccagg
     1441 cggtgccggt catttctcca ttgccgttca aaccgaaagt cccggcgtaa accggatcgt
     1501 tgagcgaatc atggccgcgc ggaatgatcg cggtcacgcc ggtgcgaacc ggacctttgc
     1561 cgacctcgag ttttccctcg ccgctgatca gggtcgtgaa gccaacttcg acgccggcga
     1621 cgtccgtgat tgcgttgagt tttcccggcg tgccgccaaa tgggatgccg agcgcgcggg
     1681 cgcgcatgtt ggttgtcggc ggcggcgttg tttgcggttc gcgcgcctgc gcgccaagcg
     1741 cgaagaggag gaggcaggcg agaatcttgg tgttcatagt tcagagcgac ggctgcgtga
     1801 cgccatattt cagcagaaga tgatcgatgt aaacgctgta gcgctgggcg agcgcggcgc
     1861 tttcgaggaa gaggatgttt tccgcattga actgcgcgct ccgcgaaaaa ttgtaggaac
     1921 cggtaatgac cgtgtcgtcg atgatcagga ctttgttgtg catgaaatcg tgccgcccgg
     1981 tcggcgtgta gggcgtggaa ttttttccca ccagatgagc gcgcgcgatg atctgccgca
     2041 ccgcggggat tttccactga ttcgaaggca cggtctgcca ttgtcgaaag actccttcca
     2101 tctgcgtgcg atcgaagatc ccatcgacct tcactcgccc cgcgcccagt aaatgatcga
     2161 gcgccgagat gagcgtgccg gaattgataa gcagactgca aagacgcacc cgttgttgcg
     2221 ccgcgcgcac tcggcgcgcc acttcggcat cgatctcgag accgcatccg ggcgaaaaca
     2281 tgacccgcac tgtcacgggc tcgcccgcaa aacggagcgt cgcgctggtg gttttgagat
     2341 ggccggtgtt ttcgaagttt cccttctccc agatctgctc gaaatcctct gcgtaaaaag
     2401 cggccaggac cggcgagtca agctcgatga cgttgttttc catgagcgtg aacgcgtcat
     2461 tcgttaggtt gagcgaaccg gtccagaccg cagcgccatc gcgcacgatg aatttgtcgt
     2521 gcatcaattt cactccgccg attcggcgcc agggaaagcc gagcccctgc acaaaagcgc
     2581 ccgtgccagg tggcgccggg tcctgtccgc cggccacgtt cggtgtctcc ggcttgtcac
     2641 catcgtagca aatccgaatc tggaccccgg ccgcggcgcg ctcctccagc gcctgtaaaa
     2701 gttggagccg caactcgtcg tcgagtcgca tatcgtaaac cgccaggtcg aggctccgtt
     2761 gcgcctgacc gaggaagtgg gtcagccgtc ccatgacgga ggcgccggat tgctcgcctt
     2821 cggccagaaa gaagacggaa agttgatcgt cggattcgtc tctttggatc gtggattcca
     2881 tttctgcttt cagcgctggc catcctatcg cgaggcgagc ggaacgcacg aaggtttccg
     2941 gagttctcgg atttcggaca ggattaacag gatgttgcag gatgaaaacg aagtcgcgct
     3001 cgtctcccaa aaatcccaat catcgtgtaa attctgtcct atttccgatt ttctgttttc
     3061 gcgtcatcgg ccaggcacta gattgggcgc gtgagctatc aggttttcgc gcgcaagtat
     3121 cggccgcaga cgttcgacga tctggtcggc caggcgcacg tcacccagac gctgaagaac
     3181 gcggtcgaac aaaatcggct cgcgcacgcc tatcttttcg tcggcccgcg cgggattgga
     3241 aaaacctcga ccgcgcgcat tctggccaag gcgctcaact gcattcatgg cccgaccatt
     3301 acgccgtgcg gggtgtgcga cagttgcaag gaaatcaccg ccggtaacag ccttgatgtc
     3361 ctcgagatcg acggcgcgag caacaataag gtcgaagagg ttcgaactct caccgagaac
     3421 gttcgttacg cgccagtcaa agggaaatac aaaatttacg tgatcgacga agtgcacatg
     3481 ctcactccgt cggctttcaa cgcgctcctc aaaacgctcg aggagccgcc cgaacatgtg
     3541 aaattcatct tcgccacgac cgaaccgcag aaggtgttgc cgaccatttt gagccggtgc
     3601 cagcgtttcg atctccaccg catcccggcg aacctgatcg cgcagcatct gcaattcatc
     3661 gcgaaaaagg agaagataaa actcgattca gccgcggcgc acgccatcgc gcgcggcgcg
     3721 gatggcggcc tgcgcgacgc ggaatcgatg ctcgatcaac tggtcgcgtt ctgcggcgag
     3781 acgatcgcgg agagcgacgt gctgaatgtc tttggcttca cgagtgagca aacgatcgcg
     3841 cgattcacgg agaaaattct gcgcggcgag acggccgatg cgctcggctt gctcgacgaa
     3901 caatcggcgg cgggcaagga gatgatgaaa ctgatgtccg acctgatcgc gcacctgcgc
     3961 gacttgctcg tcttcaaggt gaaaccggac gcgctcgccg aggaagccgc gccggagttg
     4021 caatcttcgt taggcgaaga ggcggctctg atcgagaccg accgcctgct cgagctgatt
     4081 gagcagtttg ccgaggccga agggcgaatg aaatgggcgc ctaacaagaa actgcatttc
     4141 gaggttgcgg tcatcaaagc gattcaaacc ctcagccagg tcactctgac cgaggtgatc
     4201 gaaaatctca ccgccctccg cggcggaggc gcatccatcc catcggtccc aaaaccaccc
     4261 gtcgcggaga agaaagctgc gcccaaagcc ggacagccaa ccgtgctggc cgccactcct
     4321 gatcccactc ccgggggatc gccgcacgca ggcgcctccc acattccggc cgcgacgccg
     4381 cctgtcgaaa tggcggcgat ctggccgaag gcggtgcaat tggcctccgc gcgccggccg
     4441 ctgatcaaaa gctggatcga ctcggccagc ctgcttggga cggaaggccg cacggttctg
     4501 ctcggcttcg cgcccgacca gaagacagtg atggaatcgc tttcgcgacc gtcgaatcga
     4561 agtttcctcg aagcactgct caaggaactc accggctccg attggacggt caagttaagt
     4621 ctcgcggagg gcttgccgcc gaccccgccg cccgacgaga ctcggccgga gctcgaaccg
     4681 gtgagggccg ggcctaacga ctcgctcgaa tccttcagga acgatccctt aatcaaagaa
     4741 gccctggaaa ttttcaaggg cgaaatcaaa tccgttacta cttgagccat gaatctaaac
     4801 aagttgatga agcaagcgca gaagatgcag gagcagatgg ccaggaccca ggccgagctc
     4861 gaggaaaaaa ccgtcgaggt cagcgccgcg ggcgggaagg tgacggtggt tgcgaacggc
     4921 gcgggcgacg tgctctcgat caagatcgcg aaagagatcg tcgacccgga ggacgtggag
     4981 attttggaag acctgatcct gagcggcgtg caacaagcga tcgcgcaagg caaggagttg
     5041 gcgagcagcg agatgaccaa gatcaccggc ggaatgcccg gattaagcgg ccttggcctg
     5101 tagggcggcc cgggaaagaa gtgtcaacca attgtggcag gcggcacgac agggaggttg
     5161 gggcgaattc tgctcaccaa tcagctcgct gaatcggttt ggaaccggac gcgcaatcgg
     5221 cgctgaacat aatctatctc atcaccctaa ttttcagggc tacatgcagg ggccggtgca
     5281 ctccgccgaa agacctggcg ttccgaagcg gtatcttttg agctttcgcg gagactgaag
     5341 aaagagtcag ggattaacca actgcacccg gtaaaagcgg ctcggcagat cagttgaatc
     5401 ggtcgtcgaa gtcgttagtt ggcccgggcc ggctgtgatc ggcgagttaa gaggcgccca
     5461 cgtccctcca agttgatcgc agctctcagt ttggtaagtc ctaccaggaa cagatgccca
     5521 gctgatcgtg gtgacttcgc cggtgcgcgt aatttgggtg atgccaaagc cgaggatggg
     5581 atcgaggcga tcgtaaatgc cgtcagcgtt ctcatctttg agcgccgcga tggataacgc
     5641 aaggaattca tccggatcaa tattgccgtc gccattccca agcggacatt gcccggcgag
     5701 gctgccgcca tcggcggtcg cgtaggccgc cgccgcaacg taaacggtgt ccggcacggc
     5761 tccgaaggcg gccgcgagat cgatcgtggc ttccatctgg ccactgctgg tcgcggactt
     5821 gacggcgagc gatgaaagag gagcgttgaa ccagccgcag aaatcgctcg tactttcccc
     5881 accgacgaag ggcttcgagg gatcgatcgc gatcaggccc gtcttcgccc atggcgccag
     5941 ggcggaggcg gcaggaagga gttcgtcggt tacgaagata aagttgtcgt taggctcacc
     6001 atcgcctccc ggggacgatg tcgccacata gagggtgctg ccgcgtaccg cggcgtagag
     6061 ggtgagtccg tcggcgtcct ggagataacc ggcgaagtcg gcctggccat caagattgaa
     6121 agggatcggg gtaggagagg gtgttggggt tgggctgggc gtcggggtgg gagtgagagt
     6181 aggagtgggt gttgggctgg gagtggaagt gggggtaggg gtaggagtag aagtaggagt
     6241 aggagtaggg gtaggagtgg cttggagttt cgtgcgggcg taggtgatga cactcggcaa
     6301 atttctgacg tcaacctcat aaatttcgat gtagttgacg ccgtagctga cgcccgcgtc
     6361 gacgctggtg gtgaggatgg tcgccgggtc gccgggaatg ccgttgttga cccgataggt
     6421 ggtgtcccca taaacgccag ccaacatctg cgcgcccaca tcgggttgac tattccagag
     6481 gacattccag acagaatcat ccgggcccgg cgcgacagga ttaaaggtgg aaaggctatt
     6541 gacctggaca atcagccttc cgggccattt gccgcgcgac aggctaatcg tggtggccgc
     6601 ggcgtaggtc gcggtgggat caagattggg tcccgtgttg gtatagccgt cgccaccgat
     6661 ggccagggtc acgtattggt tcgggaaagc cgccatggtg gcgtcaatga tctgttgccc
     6721 ggcatcgagc agcatttccg tggtgta
//
LOCUS       DATMNS010000022        21356 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3238752, whole genome shotgun sequence.
ACCESSION   DATMNS010000022 DATMNS010000000
VERSION     DATMNS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 21356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21356
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3238752"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            534..1409
                     /locus_tag="VNW28_00865"
     CDS             534..1409
                     /locus_tag="VNW28_00865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXA08499.1"
                     /translation="MRSMTGYGRGESERGGAKISVEVNSVNRKQSDIVINLPRELSAL
                     EPRVREVVSDRLARGRTNVVVAYDPSAHASRRLALDGALARSYHDGMCSLQKELGVTG
                     EITIAMILQAPGVLRFSDDGLEPDKVWPALEEALGAALDDLIKMRAREGKHLAKDLIH
                     RLKVVRQALKEVRKMHPAVAEKYRATLVERINKAGLDLAPGDERLLKEVAFFADRSDV
                     SEELTRLESHLAQFAHHLRKDEPVGRTLEFITQEISRELNTLGAKAGDAEISRHVLAC
                     KSEVEKIREQIQNLE"
     gene            1402..2013
                     /gene="gmk"
                     /locus_tag="VNW28_00870"
     CDS             1402..2013
                     /gene="gmk"
                     /locus_tag="VNW28_00870"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXA08500.1"
                     /translation="MNKEFSRYGILFVVSAPSGAGKTTLCDALRQTPDFVYSVSCTTR
                     APRAGEIEGEDYHFMSEEDFRARIAADEFLEHANVHGKYYGTLRDPILTDLHKGVDVL
                     IDIDTQGAAAIRAFDDAFVRQALADVFIMPPDLEELRRRLTKRGTETPAQIKQRLQTA
                     AREMELWRDYRYTIISRSMEEDLLKFRQIMGAERILSSRLRMG"
     gene            2037..2603
                     /locus_tag="VNW28_00875"
     CDS             2037..2603
                     /locus_tag="VNW28_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HXA08501.1"
                     /translation="MKQKKRSVVLGVTGSIAAYKAAELASLLVKQGHDVFVVMTKDAT
                     EFITPLTLQTLSKNPVTTGFYDEKQSWHPGHIALADRANLLLIAPATAHLIAELAHGL
                     AGHPLAAIALATRAPILLAPAMNGKMWDHPATKENVATLGARGIEFIGPEAGMLACGY
                     EGLGRLWKVDEIAFRAEFLLARQDNLIA"
     gene            2600..3253
                     /locus_tag="VNW28_00880"
     CDS             2600..3253
                     /locus_tag="VNW28_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HXA08502.1"
                     /translation="MKFVVTAGPTREPLDPVRYLSNRSSGKMGYAIAAAALEEHHSVT
                     LISGPVALPAPAGAEVMPVTTGDEMFDAVAARLAPCDVLVMCAAVCDYQPAHYNPEKM
                     KKQRGPFSLALVPTRDILASLTSRPERCFVVGFAAETQELEANAVRKLREKNCDLLVA
                     NDVSRADVGMDSDENELLIFFPNGERRRLPRAKKKELARALLKIILDAREKSLTKKS"
     gene            3560..4306
                     /locus_tag="VNW28_00885"
     CDS             3560..4306
                     /locus_tag="VNW28_00885"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXA08503.1"
                     /translation="MIHFVDVATEAARAAGDLLCSHFQKPLAVHAALAHDIKLEVDVL
                     AQDLITRILLERFPEHALYGEEGIVGDQTSDYQWVVDPLDGTVNFFYGIPHFCVSIAL
                     RFRREIMVGVIFDPIRNELWTTQNKEAPLLNGREFRVSPRAELAQAILSVGLSKTTAT
                     IDAGFPLLEKMVHRARKCRLLGSAALDMAYVACGRLDAYIEQGISLWDIAAGWILVEN
                     AGGRVELQPRSDAPDKFSIIASNGLLDLQL"
     gene            4317..4793
                     /gene="ispF"
                     /locus_tag="VNW28_00890"
     CDS             4317..4793
                     /gene="ispF"
                     /locus_tag="VNW28_00890"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HXA08504.1"
                     /translation="MFRSGIGYDAHRFGDGRTLVLGGVEIPYARGLAGHSDADVLTHA
                     IADALLGAVGERDIGHHFPNTDESIRGISSLEILRRVGQILAEKECRVANVDATLIAE
                     APKIGPHLEAMRSRISAALGIDPARVGIKATTNEGMGALGRGEGMAALAVASVEQE"
     gene            4793..6202
                     /gene="cysS"
                     /locus_tag="VNW28_00895"
     CDS             4793..6202
                     /gene="cysS"
                     /locus_tag="VNW28_00895"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXA08505.1"
                     /translation="MALRFFNTYSRAIEEFRPLDPDGRAVKMYTCGPTVYSEAHIGNF
                     RAYIFEDLLQRHLELRGFTVERVMNLTDVDDKTIRGSRAANVPLAEFTAPFKEAFYED
                     LDTLQIKHADSFPAATEKRFVEAMIAMIAKLMTRELAYQAEDKSVYFRINRFPKYGQL
                     AHFDVAELRSTGRVKSDEYDKEHVGDFALWKAWDEEDGAVWWESPWGRGRPGWHIECS
                     AMATTLLGEQLDIHCGGVDNIFPHHEAEIAQSEGCSGKQFVRYWLHCAHLLVEGQKMS
                     KSLGNFYTLRDVLAKGYRGREVRYALLRVHYRAPLNFTWEGMEEARQALARIDEWLRR
                     LREAARAGNSPANRADALEDDKFAVALDDDLNISAALGHLFEQIRESNRELDGGIDAA
                     RALAWLQWWERINRVLALEAEAEKPSEEIARLAEERMHARLAKDWQKSDELRDALQAR
                     GWDVRDTKDGQLVTRRAGA"
     gene            6189..6905
                     /locus_tag="VNW28_00900"
     CDS             6189..6905
                     /locus_tag="VNW28_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine diphosphorylase"
                     /protein_id="HXA08506.1"
                     /translation="MPAPNEMFSPAEFFELDHTAHSKLFEKDKFVWEALKQIPSYLQF
                     RLKPAFLGELVGKPYISGSVFIGSGTIVEQGAMLKGPAWIGENCHIRHGCYVRENVIV
                     GNGVVMGNSCEFKNCIIFDEAQIPHFSYVGDSILGYRAHLGAGVILSNVKLDHAEISV
                     LATDGLIATGLKKFGAIVGDRTEIGCNAVLNPGSVVGRDCIIYPGTNFRGVLPGNSIV
                     KIRQTSQVLSRRQPKDSEPG"
     gene            complement(6941..7025)
                     /locus_tag="VNW28_00905"
     tRNA            complement(6941..7025)
                     /locus_tag="VNW28_00905"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6989..6991),aa:Ser,seq:gga)
     gene            complement(7087..7908)
                     /gene="hemQ"
                     /locus_tag="VNW28_00910"
     CDS             complement(7087..7908)
                     /gene="hemQ"
                     /locus_tag="VNW28_00910"
                     /EC_number="1.3.98.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006785 - heme B biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide-dependent heme synthase"
                     /protein_id="HXA08507.1"
                     /translation="MDQTPAPLIPQEGWHCLHLFYRIEYGQWQLLSAAEQRAAKIALS
                     TLVQETRALASTQLLTLSQVTPKADLGFMLVTPDLQTANRIEKQLTLSLGADVLVPVY
                     SYLSLTEESEYTTSDEQYAATLEIEQKLRRDTPEFAKGMEEFRERMKKYRHDRVYPTL
                     PDWPVVCFYNMTKRRGEQHNWYSLPFAERRTLMGGHARVGRQYAGKVKQLITGSTGLD
                     DAEWGVTLFAHDTFQIKAIVYQMRFDEVSAAYADFGEFYIGIQLPLDELFRRLQL"
     gene            complement(8072..8875)
                     /locus_tag="VNW28_00915"
     CDS             complement(8072..8875)
                     /locus_tag="VNW28_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08508.1"
                     /translation="MKLLDSLERRFGWLAFPGLIRVVVALTALVYLLTFLNPNFLSVL
                     TLDPARIMQGEVWRLVTYIFIPRSIGQPGALLQPLWLIVALWFLLFIGDRLEHAWGAF
                     RLNLYFLVGMIGTTIAAFLFGAQFSNAMLASSLFFAFAHFYPDEVIYVLFVLPMKVKW
                     LAWVSAALLLFGFVSSPNSYRMALIAAFANYLIFFGPDVVRTVRNRQEVASRRRRFEE
                     NKRPEEDALHHCANCGATELTNPNLEFRVARDGEEYCLAHLPSATKVEA"
     gene            9080..10180
                     /gene="gcvT"
                     /locus_tag="VNW28_00920"
     CDS             9080..10180
                     /gene="gcvT"
                     /locus_tag="VNW28_00920"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF001567.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXA08509.1"
                     /translation="MSDAAEKRTPLYEEHVRLGAKIIPFGGWLMPVQYSSIMEEHQAV
                     RKSVGVFDISHMGELVATGQRAGEWLNTMLTNNIAKLEVGSGQYTFLLNDEAGIIDDL
                     IVYRIDDQRFLLVVNASRIEDDFAWLQSHLASDVQLENQSDEYAGLAIQGPRVGELFQ
                     AVFGDGKPMPARNQIAPFDLGGTNIRVARTGYTGEDGIEVFFAAQAAPRVWKEILEKG
                     KSLGIRPCGLGARDTLRLEMCYPLNGSDLSPAHNPLEAGLGFFVDLAKPHFTGREKLL
                     RDKEAGLSRRLVPFKMKGKSPPPRPHYRVFRDGKEAGELTSGTLSPSLNYGIGMAYLT
                     TPEPKPGEEIEIEIRGQKFPAVIEKKPLYKKS"
     gene            10177..10554
                     /gene="gcvH"
                     /locus_tag="VNW28_00925"
     CDS             10177..10554
                     /gene="gcvH"
                     /locus_tag="VNW28_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HXA08510.1"
                     /translation="MNVPDDLLYTESHEWVRREGDKIRVGITDHAQAELTDVVYVELP
                     KMGAAYEAGGTIAVVESVKAASDIYAPAKGTVLEVNKALESNPALINTDPYGEGWIYV
                     LKADDPEQLKALKDAAAYRSSIG"
     gene            10886..11809
                     /locus_tag="VNW28_00930"
     CDS             10886..11809
                     /locus_tag="VNW28_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08511.1"
                     /translation="MKKLAVVLLLVAASVAPASVINVEFKFTPFVGDPEKDKEVTIVA
                     GKAQVFINNVQVAEQEVHEEKGPVLFDAHEVGPAVWVPMSSVGPVLRKGKNKIRIEFT
                     PNDPAKTYRAQLRWAAVTDQATEEVEPGSVKATNQADEGVDDRKASKGKVVFEREFVA
                     DFALDLPWHHYPAVNSLTEDDKQKIAGLLKMRAEWFQPDFAALYKAIDENEALKVDDV
                     RKARCLEAAYQAGVRVTAPAPGELEFATTGGPEVVVTRKAGTLFGLDEKTFAPVKDEE
                     TQMCAGMVLSVIYPGHIAAVRKPDGSWAIVF"
     gene            11960..12211
                     /locus_tag="VNW28_00935"
     CDS             11960..12211
                     /locus_tag="VNW28_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HXA08512.1"
                     /translation="MPTEIKLFSREWCSWCIDAKDYLLQHGYQFTEIDVGRDREAYEE
                     MRELSDQTYVPTLVAGGEVLANFDTDQLAKFLRVHAIVP"
     gene            12212..13027
                     /locus_tag="VNW28_00940"
     CDS             12212..13027
                     /locus_tag="VNW28_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08513.1"
                     /translation="MLFGLFITLGVAVLSVALRSYQSSVSQKIGAFGILTASFLAIYY
                     ITGNWVLGLLSALSWLFLPWLEILTRIRALRLPREKTLRPKSPPSPEVFPTLDEITAQ
                     IESEGFIHVGDAGWDWEDYKQFFRLFYKEVDRAQATICLNEQHDLSFYYLRISSRGRD
                     GTIWTTWNYPLSYGLKLTPQFRINRQRPDQSFWQLYHSHKAFLRDHAVETNVIDALDE
                     DQIQQEIENDLRDQIAHNIKTGVLMQTPGGEIKYSWRGMIYLWCQFLLDLVRL"
     gene            13195..13629
                     /locus_tag="VNW28_00945"
     CDS             13195..13629
                     /locus_tag="VNW28_00945"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXA08514.1"
                     /translation="MNETAPRIQTEVQVMFFDTDCAAVVHNIAYLRFIETARTLLAEQ
                     LGMKLTEMAENRRYPVVVRTEIDYRRAAKLGDRLRIDGWLDRLERVRFWCVFQIRRIE
                     DDALIVDCRQTLALIQLPEAKLLRLPEEWERFRAPEAADVPR"
     gene            13674..15470
                     /locus_tag="VNW28_00950"
     CDS             13674..15470
                     /locus_tag="VNW28_00950"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXA08515.1"
                     /translation="MSTGNPALTLDEAFLSPRALEPPPIRRALLVFLLCLAALLHVAT
                     SGWGDLYNETDGQYAGAAREMLQSHQYLTPTNDGIPRLQKPPLLYWAIALSLKAFGLT
                     TAAVRLPIALGTVVAIALTFLIGDRLGGPWRGFLAGLIHLCACGTFLLGRIVMPEPVF
                     SALIAGSIYCALRGYERRQGRRGWFIGFWFCVALACMTKSLHGLLYPAAILGLLACFY
                     REARLRFRELLRWDGILVFLLLVAPWHLWLEWKHPGFLSQFTAQEWLVHLAGKADQGH
                     SYDNVPRHVFLALHLAWWFPWTVAILPGVFCAWRRVFRPREIEFADALPLCWMAVVFI
                     PLFFIGQRQDYYSMSMWGGFAIFAATAWERMPRWTRVVGVSGVLVIGLVAGFVAWRLP
                     ELLSGVSGHWGVTAARSTAWRTLLDIPTSTWLGFRSMFVVIGLALVLFSAIALWFVWR
                     ERPRLALMALAAAMVPIGFSMMDGVARMAPYFSLAEAANFIQERIAPNDKIVYEGPMH
                     VGSSLLFYLGRKFYLVNQDPASEPGAALGPTPDIFLDEKTVLEAWKGADRFYLLVERE
                     RLPHWHALLRANGAAPEELTTCGTTVLLSNHR"
     gene            complement(15507..16076)
                     /locus_tag="VNW28_00955"
     CDS             complement(15507..16076)
                     /locus_tag="VNW28_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXA08516.1"
                     /translation="MDCILRPRRLSGAANAWLALGFAAVLLCALGLLSCRGIDWFLLK
                     RSLRGRFGEENWITTQQLADWLADKKRPAPVLLDVRTPAEWKVSHLPGARQVDPQADA
                     SAAAGKVARDAPIVTYCAVGYRSGALAQRLRAAGYTRVQNLEGSIFEWANEHRPLARD
                     GERVTRVHPYSALWGRLLQPEVRAPLTQK"
     gene            16141..16296
                     /locus_tag="VNW28_00960"
     CDS             16141..16296
                     /locus_tag="VNW28_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small basic protein"
                     /protein_id="HXA08517.1"
                     /translation="MSQHRSLKGASTIAAKRNVLKRFERVELLKKRGQWKETSKVIGL
                     PKTKPDL"
     gene            16355..17080
                     /locus_tag="VNW28_00965"
     CDS             16355..17080
                     /locus_tag="VNW28_00965"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXA08518.1"
                     /translation="MRLNRFLAAAGLGSRRHCDELIAEGRVTVNGQPCTNFSTQVEAS
                     DHVKVNHRLVRPAETLTIALHKPAGFVSTRRDPNATDTIYDLLPAKFSRLFNVGRLDA
                     QTEGLLLLTSDGDLAQHLTHPSYKVEKEYEVTLDRRWDPKRGQKLLDGVFLDGRRARL
                     ARLQPIGPTRLRVVLQQGMNRQIRRMFYEIGYEVERLVRTRIGHLRLGELPRGSWRPL
                     TKSELARVRSQPLSGGRSAGPVF"
     gene            complement(17118..19634)
                     /locus_tag="VNW28_00970"
     CDS             complement(17118..19634)
                     /locus_tag="VNW28_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXA08519.1"
                     /translation="MAWRDSRASRSRLLLFSISISLGVAALIAIGLFRASLAQAIDDQ
                     ARSLLGADLVVESSRPFTPEQEQMLHSLGEIQAREVRFNTMALFPKAGGTRLVHVRAL
                     GGAFPFYGRMETAPATAAKTFRTGGQAVPEESLLFQFRAKPGDPIKIGEATFTIAGAL
                     TKMPGEASPAASFAPRVYIPLQNLAQTNLLKPGSLARYLNFVKFAPNVDVPGEVKKLA
                     PRIEHFGLEYDTVAKRKKDLGESLDNLYRFLNLVGFISLLLGAVGVASAIQAHLQQKV
                     RTAAILRCLGAAGRTTVIVYLLQAMAMGFVGVLTGAALGLTIHQTLPGALQKYLPFVL
                     PTAIAWLPVLRGMAVGLGVCLLFALPPLLRFRRVSPLIALRSSVEEGAAPVARDLVIW
                     LVYFAIAASLTAFAISQAEAWTDGLFLAAGLATAVAVLIGVAKLLIFLVRKLLPQRWS
                     FVLRQGLANLYRPNNRTLLLTLSLGLGTFLLLTIYLTRDVLLTQFRSIGARDQPNIFL
                     FDIQPGQTGPVAGLVRQLGFPVLQQAPIVTMRLLEVKRRKAAEMLKDPQNKAPEWELE
                     REYRSTYRAQLSETEKITAGKWVGRVDYHPGDTVPISVEQDIAKDLGLTIGDALAFDV
                     QGVPIKARVASLREVDWKRFQTNFFIVFPTGVLEKAPTFNVLVSRVPTPAASARLQNA
                     VVARFPNVSALDLTSVIQTVDSILTKVALVIRIMSLFTVGTGLIVLGSTIWSGRYQRL
                     KESVLLRTLGASRWQIWKILGTEYLFLGLLASATGIVLALVASWALAVFTFKLAYTPA
                     LLPVVIAGSIVSALTVAIGLLTSYGLGNTPPLAILRDELE"
     gene            complement(19774..20448)
                     /locus_tag="VNW28_00975"
     CDS             complement(19774..20448)
                     /locus_tag="VNW28_00975"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA08520.1"
                     /translation="MNSCPILEVRDLTKSYATGAGRLTVLRDISFTLEQGATCSILGP
                     SGSGKTTLLGLAAGLDLPTSGSVLLNGIALSEISEDERARVRNEHVGFVFQNFQLIPT
                     LTALENVTVPMELRGDRSARALALDLLQRVGLGDRSDHYSTQLSGGEQQRVALARAFI
                     NRPKVLFADEPTGNLDAETSQNVIEIMLELNRSAGTALVLVTHDPEVARLTERTIRLR
                     NGAMVG"
     gene            20499..21131
                     /locus_tag="VNW28_00980"
     CDS             20499..21131
                     /locus_tag="VNW28_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HXA08521.1"
                     /translation="MRKLMRRGFVVLFCALACASPARGGTILFLGDSLTAGLGVEQGQ
                     AFPALIQEKIREKNLPFDVINAGISGDTTAGGLARLDWVLQKKIDVLVLALGANDGLR
                     GLPVAQMKANLQAIIDRVKAKNPAVKIVIAGMRMPPNLGGAYSDAFREVYGDLARENH
                     AALIPFLLEGVGGHDDLNQADHIHPTAAGHKVVAENVWRVLGPLLGNGGS"
     gene            21131..>21356
                     /locus_tag="VNW28_00985"
     CDS             21131..>21356
                     /locus_tag="VNW28_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXA08522.1"
                     /translation="MRLDGCNALITGASAGIGREFARQLGDRAATLVLVARRQARLEE
                     LRAELTERHPQLRVEIRPTDLSQHEAVDALL"
BASE COUNT         4208 a         6318 c         6758 g         4072 t
ORIGIN      
        1 tttcgctggc gccgccgccg gcttctgggg cggactcctc ctcagccgca gccttccaag
       61 gattgatgaa gcgtaaggcc aggcagcagg cgggaaaagc aacgtctgcg ggccggatct
      121 gcgctacgga ttactccgcg aacggaaagt atgcccaggc gcaatacaat ttgggacgtt
      181 tgctcgcgca atttgggcga caagatgagg cgcgcccgca tctgaccgaa gcattgcgac
      241 tctaaccgga ctacccggag gcgaggcaac gtttgctcga actcggcgcg gccgcgccgc
      301 cctgacctgg cggagcgtca atctgcccgc cagaatttga aagagcggga cataaaaccc
      361 gtaacccccc tgtgcagtcg ccttgcctct gctccggttc tgagctcgct cttttctcaa
      421 aagggcgccc ctccgcgcgg gccgaacacc cgtcgcgcca gggccacctc tcggttaaga
      481 agccgcgcgt ttctatttgt tattcgcgcg gtatctttcg aagctgcatc ccgatgagaa
      541 gcatgactgg ttatggccgg ggcgagagcg agcgtggcgg agcgaaaatc agcgtcgagg
      601 tcaactcggt gaaccgcaag caaagcgaca tcgtgatcaa cctgccgcgc gagttgagcg
      661 cgctcgagcc acgcgttcgg gaggtggtga gcgaccggct cgcgcgcggg cgcaccaacg
      721 tggtcgtggc gtatgacccg agcgcgcacg cctcgcgccg gctagcgctc gatggcgcgc
      781 tcgcccgttc ttatcacgac gggatgtgct ccctgcagaa ggaattgggc gtgactggtg
      841 aaattacgat cgcgatgatt ttgcaggcac ccggcgtcct ccgtttctcc gacgacggat
      901 tggagcccga caaggtctgg ccggcgctcg aggaggcgct cggggccgcg ctcgacgact
      961 tgatcaagat gcgcgcgcgg gaaggaaaac atctcgcgaa ggatttgatt catcgtctga
     1021 aggtcgttcg ccaggcactc aaggaagtgc ggaagatgca tcccgccgtc gcggaaaaat
     1081 atcgcgccac gttggtcgag cgaatcaaca aggccggcct cgacctcgcg cctggcgatg
     1141 aacgtctgct caaggaggtc gcctttttcg ccgatcgctc tgacgtttcc gaggaattga
     1201 cccggctcga aagtcatctc gcgcagttcg cgcatcatct gcgcaaggac gagccggtcg
     1261 gccgcaccct cgaatttatt acacaggaaa tttcccgcga actgaatacg ttaggcgcga
     1321 aagcgggcga cgcggaaatc tcgcgccatg tcctggcctg caaatcggaa gtggagaaga
     1381 ttcgcgagca aatccaaaat cttgaataag gaattcagcc gttacggaat ccttttcgtc
     1441 gtctccgcgc cttccggggc gggcaagacg accctgtgcg acgcgctccg ccagacgccg
     1501 gattttgttt actccgtctc ctgcaccacg cgcgcgccgc gcgccggtga gatcgaaggc
     1561 gaagattatc attttatgtc cgaggaagat tttcgcgctc ggatcgcggc ggacgaattt
     1621 ctcgagcacg ccaacgtgca tggaaagtat tacggaacct tgcgcgatcc gattttgacc
     1681 gacctgcaca agggcgtcga cgttctgatc gacatcgaca cccagggcgc ggccgccatc
     1741 cgcgcattcg acgacgcgtt tgtgcgtcag gcgttagccg atgttttcat catgccgcct
     1801 gacctggagg aattgcgccg ccgtcttacg aaacggggca cggagacgcc ggcgcagatc
     1861 aagcagcgcc tgcaaaccgc cgcgcgggag atggagctct ggcgcgatta ccgctacacc
     1921 atcatcagtc gctcgatgga ggaggacctg ctcaagtttc gtcagatcat gggcgcggag
     1981 cggattttga gcagccggct ccggatgggc tgaaggatgg aagcaaacgc ctaacgatga
     2041 agcaaaaaaa gcgatccgtc gtgctcgggg tcaccggctc gatcgccgct tacaaagcgg
     2101 ccgagctcgc cagcctgctc gtgaaacagg gacacgacgt tttcgtcgtg atgacgaagg
     2161 acgcgaccga gttcatcacc ccgctcacct tgcagacact ttcgaagaat cccgtcacca
     2221 ccgggttcta cgacgaaaag caaagctggc accccgggca tattgcgctc gccgatcgcg
     2281 cgaatttgct tctgatcgcg ccggccacgg cccaccttat cgccgagctc gcgcatggcc
     2341 tggccggtca tccgctggcg gcgatcgcgc tcgccacccg ggcgccgatt ctcctcgccc
     2401 cggcgatgaa tggaaaaatg tgggatcatc cggcgacgaa agaaaatgtc gcaacgttag
     2461 gcgcgcgcgg gattgaattc atcggcccgg aggccgggat gctggcctgc ggttacgaag
     2521 gcctcggccg cttgtggaag gtcgacgaga tcgcgttccg cgccgagttt ctgctcgccc
     2581 gccaggataa tctgatcgcg tgaaattcgt tgtcacggct gggccgacgc gggagccgct
     2641 cgatcctgtc cgctatctga gcaaccgctc ctcaggcaag atgggctacg cgatcgccgc
     2701 ggccgcgctc gaggagcatc actccgtgac cctgatctcg ggaccggtcg cgctccccgc
     2761 gccggctggc gcagaagtca tgcctgtcac cacgggtgac gaaatgttcg acgccgttgc
     2821 cgcgcggctg gcgccttgcg acgtgctcgt gatgtgcgcc gcggtctgcg attaccaacc
     2881 ggcgcactac aacccggaga aaatgaaaaa acaacgcggc cccttttccc tcgcgctcgt
     2941 cccgacgcgc gacattctcg ccagcctaac gagccggcca gagcgctgtt ttgtggtcgg
     3001 cttcgcggcc gagacccagg agctggaagc gaacgcggtg cgcaagctgc gggaaaaaaa
     3061 ttgcgacctg ctggtcgcga atgacgtcag tcgcgcggat gtcgggatgg attccgacga
     3121 gaacgaactc ctgattttct ttccgaacgg cgaacgccga agacttccgc gcgccaaaaa
     3181 aaaagaactt gcgcgcgcgc tcttgaaaat aattttagat gcgcgcgaaa aaagtttgac
     3241 aaaaaaatcg tgacgattag tgacacgatg aaagtgaagt tattcacaga ctgttcacag
     3301 accgcttctg cggattgaaa ggggggattc cctaatgcca gcgaaaaaga aagctaagaa
     3361 gccagccaag aaaaagatgg cgactaagaa aaaagcatcc ggcaaaaaga aacattaggt
     3421 ccggatctcc acagaagggc ggtgacagaa ccgttcttcc tcaagttaac ttcaacccgg
     3481 gggaagaatt catttcttct cccggttttt ttgcgcaaaa acttcgcggc agctccgcag
     3541 aatcggttag gttcagcgcg tgattcattt tgtcgatgtc gcgacggaag cggcgcgggc
     3601 cgcgggcgat ctgctttgct cccatttcca gaagccgctc gccgtgcacg ccgcgctggc
     3661 ccacgacatc aagctggagg tcgatgttct cgcccaggat ttgatcaccc ggattttact
     3721 cgagcgcttt ccggagcatg ccctctacgg tgaagaaggc atcgtcggcg accagacaag
     3781 cgattatcaa tgggtcgtcg atccactcga cggcacggtg aatttttttt acggcatccc
     3841 gcatttctgc gtctcgatcg cgctccgttt ccggcgcgag attatggtcg gcgtgatttt
     3901 tgacccgatt cggaatgagc tttggacgac gcaaaacaaa gaagcgccgt tgttgaacgg
     3961 ccgggagttc cgcgtcagtc cgcgcgccga gctcgcgcaa gccatcctgt cggtcggcct
     4021 ctcgaaaacc acggcgacga ttgacgccgg cttcccgctg ctcgagaaaa tggtgcatcg
     4081 cgcgcgcaag tgtcgattgt taggcagtgc cgccctcgac atggcctacg tcgcctgcgg
     4141 gcggctcgac gcctacatcg agcaggggat cagcctgtgg gatatcgcgg cgggatggat
     4201 cctggtggag aacgccggcg gccgtgtcga actgcagccg cggagcgatg cgccggacaa
     4261 gttcagcatc atcgcttcaa acggtctgct cgatctgcag ctttaaccct aacgagatgt
     4321 ttcgctccgg cattggctat gacgcgcacc gcttcggaga cgggcgcacg ctcgtcctcg
     4381 ggggagtcga gatcccgtac gcgcgggggc tggcgggcca ttcggatgcc gacgtgctga
     4441 cccatgcgat cgccgatgcc ttgttaggag cggtcgggga acgcgacatt ggtcatcatt
     4501 ttccgaatac ggatgaatcg atccggggca tcagcagcct cgaaatcctg cgtcgggtcg
     4561 gccaaatatt ggcggagaaa gaatgccggg ttgccaacgt cgatgcgact ctgatcgcgg
     4621 aggcgccgaa aatcgggccg catctcgagg cgatgcgaag caggatttcg gcggcgctcg
     4681 ggatcgatcc ggcgcgggtc ggaatcaaag cgacgaccaa cgaggggatg ggcgcgcttg
     4741 gccggggaga aggcatggcc gcgctggcgg tggcgagcgt ggagcaggag tgatggctct
     4801 gcgttttttc aacacctact cccgcgcgat cgaggagttc cgcccgctcg atccagacgg
     4861 tcgcgcggtg aagatgtaca cctgcggccc gaccgtttat agcgaggcgc atatcggaaa
     4921 tttccgcgcc tatatcttcg aggacttgct tcagcggcat ctcgagctgc ggggattcac
     4981 ggtcgagcgg gtgatgaacc tgacggacgt ggacgacaaa acgattcgcg gcagccgggc
     5041 ggcgaatgtt ccgctggcgg aatttaccgc gcctttcaag gaagcatttt acgaggatct
     5101 cgacacgctc cagatcaagc atgccgactc gtttcccgcc gcgaccgaaa agcgtttcgt
     5161 cgaggcgatg attgcaatga tcgcaaaact gatgacgcgc gaactcgcct accaggcgga
     5221 agacaagtcg gtttacttcc ggatcaatcg cttcccgaaa tacggtcagc tggcgcattt
     5281 cgacgtcgcg gaattgcgct ccaccgggcg ggtgaagagc gacgagtacg acaaggaaca
     5341 cgtgggcgat ttcgcgcttt ggaaagcgtg ggatgaagag gatggcgccg tttggtggga
     5401 aagcccgtgg gggcgcgggc gcccgggctg gcacatcgag tgcagcgcga tggcgaccac
     5461 tctgcttggc gaacagctcg acattcattg cggcggggtg gacaacattt ttccgcatca
     5521 cgaggcggag atcgcgcagt cggaaggctg cagcgggaag cagttcgtcc gctactggct
     5581 gcattgtgcg catcttttgg tcgagggtca gaagatgtcg aagtcgttag gcaattttta
     5641 caccttgcgc gacgtgctcg cgaaaggcta ccgcggccgc gaagtccgtt atgcgctcct
     5701 tcgggtgcat taccgcgccc cgctcaattt cacctgggag ggaatggagg aggcgcgcca
     5761 ggcgctcgcc cggatcgacg aatggcttcg ccggctccgg gaagcggccc gggccggaaa
     5821 ttcgccggcc aaccgcgccg acgcgctcga ggacgacaag tttgcggtcg cgctggatga
     5881 cgatctcaac atctcggccg cgctcggcca tttgtttgaa cagattcgcg agagcaatcg
     5941 cgagctcgac ggcgggatcg atgcggcccg cgcgctcgcc tggttgcagt ggtgggagcg
     6001 aatcaaccgc gtcctcgcgc tcgaggcgga agcggagaag ccgtcggagg agattgcgcg
     6061 cctggcggag gagcggatgc acgcgcggct tgccaaggac tggcaaaaaa gtgatgaatt
     6121 gcgcgacgca ctgcaggccc gcggttggga cgtccgcgac acgaaggacg gccaactcgt
     6181 cacgcggcgt gccggcgcct aacgaaatgt ttagcccggc cgaattcttc gaactcgatc
     6241 ataccgcgca ttcgaagctt ttcgagaagg acaaattcgt ctgggaggcg ctcaaacaga
     6301 tcccgagcta cctgcagttc cggctcaagc ccgctttcct cggcgaactg gtcgggaagc
     6361 cctacatcag cggctcagtt ttcattggga gcggcacgat cgtcgagcag ggcgcgatgt
     6421 tgaaaggccc ggcatggatc ggagaaaatt gccacatccg ccacggctgt tacgtgcgcg
     6481 aaaacgtcat cgtcgggaac ggcgtggtga tgggcaactc gtgcgagttc aaaaactgca
     6541 ttatctttga cgaggcgcag attccgcatt tcagctacgt cggcgattcg attctgggtt
     6601 accgggcgca cctcggcgcg ggcgtgatcc tgtccaacgt gaaactcgac cacgcggaga
     6661 tttcggtgct cgcgaccgat ggcttgatcg caactggcct gaagaaattc ggtgcgattg
     6721 tcggcgaccg gacggagatc ggctgcaacg cagtcttgaa tcccggttcg gtcgttggcc
     6781 gcgactgcat catttatccg ggcacaaatt ttcggggcgt gctgcctggc aacagcatcg
     6841 tgaaaatccg gcaaacgtcc caggtgctga gccggcggca gcctaaagat tcggagccgg
     6901 gttagagggt cgcgggcggc gacaagcgag agcggtccgg cggatgggca gggattcgaa
     6961 ccctggttgc cttgcggcaa acacgctttc caggcgtgcg cattagacca ctctgccacc
     7021 catccgtttt cagctcgtgc tcctgctcgt gctcgctggt caggcaattt cgaaacgagc
     7081 acgaaattac agttgcaggc ggcggaacag ttcgtcgagg gggagttgaa tgccaatgta
     7141 aaattcgccg aagtcggcgt aggcagcgct gacctcgtcg aagcgcatct gatagacgat
     7201 cgccttgatc tgaaaagtgt cgtgcgcgaa tagcgtcacg ccccattccg catcgtcgag
     7261 tccggtcgag ccggtgatga gttgtttgac tttgccggcg tattggcggc caacgcgcgc
     7321 gtgtccgccc atgagcgtgc ggcgttctgc gaagggaagc gagtaccagt tgtgttgttc
     7381 gccgcggcgt ttcgtcatgt tgtaaaagca gacgaccggc cagtcgggca gcgtcggata
     7441 gacccggtcg tgccgatatt ttttcatccg ctcccgaaat tcctccatcc ctttcgcgaa
     7501 ttccggcgtg tcgcgcctca gtttctgttc gatctcgagg gtggcggcat attgctcatc
     7561 gctcgtggtg tattcgctct cttcggtgag tgagagataa ctgtagacgg gaacgaggac
     7621 atccgcgcct aacgagagcg tgagctgctt ctcgattcgg tttgcggttt gcagatccgg
     7681 cgtgacgagc atgaaaccga ggtccgcttt gggcgtgacc tggctgaggg tgagtagttg
     7741 ggtcgatgcg agcgcgcgtg tctcttgcac gagcgtcgaa agcgcaattt ttgccgcgcg
     7801 ctgctcggcg gcactgagca attgccattg gccgtactcg atccggtaga aaagatggag
     7861 gcaatgccag ccctcctgcg ggatgagcgg cgcgggagtc tggtccatgc gcgagtttcc
     7921 gtgggcgcag cgacggggtc aagccgcggg caaggcgtaa gaaaaagagg ggaaaacgtt
     7981 caacgtccaa ctttcaacgt tcaacgtcca agtaagagaa gaccaccgcc acacactttc
     8041 tgcgttgaac gttgaacgtt ttcttcagat ttcaggcttc caccttagtc gcgctcggga
     8101 gatgggcgag gcagtactcc tcgccgtcgc gcgcgacgcg gaattcaaga ttgggattgg
     8161 tcagctccgt cgcgccgcag ttcgcgcagt gatgcagcgc gtcttcttcc ggtcgcttat
     8221 tttcttcaaa ccggcggcgc cgactcgcga cctcctgccg gttgcgcacc gtgcggacca
     8281 cgtccgggcc gaaaaagatg aggtaattcg caaaggccgc gatcaaggcc atgcggtagg
     8341 agtttgggct ggagacgaaa ccgaaaagca aaagcgcggc cgagacccag gcgagccatt
     8401 tcaccttcat cggcaaaacg aagaggacgt agatcacctc gtcgggataa aagtgggcga
     8461 aggcgaaaaa gagggaagag gcgagcatgg cgttggaaaa ttgcgcgccg aagaggaagg
     8521 ccgcgatcgt ggtcccgatc atcccgacaa gaaagtatag gttgaggcga aaggcgcccc
     8581 aggcgtgctc cagtcggtca ccgatgaaga gcaggaacca gagggcgacg atgagccaga
     8641 gcggttggag caatgcgccg ggctggccga tcgaccgcgg aatgaagatg taggtcacca
     8701 gccgccagac ctcgccctgc ataatgcgcg ccggatcgag cgtcagaacg gagagaaaat
     8761 tgggattgag aaaagtgagg agataaacca gcgcggtgag cgcgactaca acgcggatca
     8821 atccgggaaa agcgagccag ccgaaacggc gttcgaggga atcgagcaat ttcatcgtcg
     8881 tgccaccgta gaggcgggct ggtggcagtt caacaatctg ggatggggga ttctcaatct
     8941 cgtgctcctg ctcatgctcg tgctcgtaat cgtcctcctg ctcgccctcg aaaaagattc
     9001 gattgcgagc cgatcacgag cccgaggcgg cggggcggag cgaattgaca gcaggagcgc
     9061 aacatctagg ctggccgcga tgagcgacgc cgcggaaaaa cggaccccgc tttacgagga
     9121 acacgtcagg ctgggcgcga aaatcatccc ctttggcggc tggctgatgc cggtgcagta
     9181 cagcagcatc atggaggaac accaggcggt gaggaagagc gtcggtgtct tcgatatttc
     9241 gcacatgggc gagctcgtcg cgaccggcca gcgggctggc gaatggctca acaccatgct
     9301 gacgaacaac atcgcgaagc tcgaggtcgg ctccgggcag tatacctttt tgctgaacga
     9361 cgaagccggc atcatcgacg acctgatcgt ttaccggatc gacgaccaga ggttcctgct
     9421 cgtcgtgaac gcgtcgcgga ttgaggacga tttcgcctgg ctgcaatcgc atctggcgtc
     9481 ggacgtgcag ctcgaaaacc aaagcgatga gtacgccggc ctggccattc aggggccgcg
     9541 ggttggagaa cttttccaag cggtcttcgg cgacgggaaa ccaatgccgg cgcgtaatca
     9601 gattgcgccg ttcgatctcg gcgggacgaa catccgggtg gcgcgcacgg gctacacagg
     9661 ggaggacggg atcgaagttt ttttcgccgc gcaagccgcg ccgcgggtct ggaaagaaat
     9721 tctggaaaag ggaaaatcgt taggcattcg tccctgcggg ctcggggcac gcgacacttt
     9781 gcgcctcgag atgtgttatc cgctcaacgg ctctgatctt tccccggccc ataatccgct
     9841 cgaagccggg ctcggctttt tcgtcgacct ggcgaaaccg catttcaccg gccgggaaaa
     9901 actgttgcgg gacaaagagg caggcctctc gcgccggctt gtccctttca agatgaaggg
     9961 gaaaagcccg ccgccgcgtc cgcattaccg cgtcttccgc gacggcaaag aagcgggcga
    10021 gctgaccagc gggacgctct cgccgagctt gaattacggg atcggaatgg cttacctgac
    10081 cacgccggag ccgaagccgg gcgaagaaat tgagatcgaa attcgcggcc agaaatttcc
    10141 ggccgtaatc gaaaaaaagc cgctctacaa aaaatcatga atgttccaga cgaccttctc
    10201 tacaccgaat cgcacgaatg ggtccgccgc gaaggcgaca aaatccgggt cgggattacc
    10261 gatcacgcgc aggccgagtt gaccgacgtg gtttacgttg agttgccgaa aatgggggcg
    10321 gcgtatgaag ccggtgggac gattgcggtg gtcgaatcgg tcaaggcggc gagtgacatc
    10381 tacgcgccgg ccaagggcac ggtgctggaa gtgaacaaag cgctcgaatc caatccggcg
    10441 ttgatcaaca ccgatccgta tggcgaaggt tggatttacg tcctgaaggc ggacgaccct
    10501 gaacagttga aggccctgaa ggatgcggcc gcctaccgtt cgtcgatcgg ctagccacta
    10561 cctctcccgc gcagcggagc cgtcgcaggg agccgctaat cattggccac cgaacggcag
    10621 cgcgccgtcc gggcgaaatc tccgcaccgg cctttcgctc tccgggctgt ttgctttcct
    10681 cgttctgacc ggttacgggc tttactagat cggggacgat catcttcgct ccctggacga
    10741 gcgccgccca tccttgggtt gggttggtgc tgccgccggt catggcccag cacgtttggc
    10801 gcgggagaaa gacgcgtccc ggccgcccgc ggccacgcgt ggaataagat tgttttcccg
    10861 gtcgaaatgg ggcaaaaaac gaccgatgaa aaagctcgct gttgtccttc ttttggtcgc
    10921 ggcgtcggtc gcgccggcca gcgtgattaa tgtcgagttc aagttcacgc cgtttgtcgg
    10981 cgaccccgaa aaggacaagg aggtcacgat cgtggcgggt aaggcgcagg ttttcatcaa
    11041 taatgtccag gtagctgagc aggaggtgca cgaggaaaag gggccggtgc tcttcgatgc
    11101 gcatgaggtg gggcctgccg tctgggtgcc gatgagctcg gtcggcccgg tgctgcgcaa
    11161 aggaaaaaac aaaatccgga tcgagttcac gcctaacgac ccggcgaaaa cctatcgcgc
    11221 gcaattgcgc tgggcggcgg tcacggacca ggcgacggaa gaggtcgagc cagggagtgt
    11281 gaaggcgacg aaccaggcgg acgaaggggt cgatgatcgc aaggcgagca aagggaaggt
    11341 ggtttttgag cgcgagttcg tggcggattt tgcgctcgat ctgccgtggc atcactatcc
    11401 cgcggtcaac tcgctgaccg aggacgataa gcagaagatt gccgggcttt tgaagatgcg
    11461 ggcggagtgg tttcagcctg atttcgcggc gctctacaag gcaatcgatg agaatgaagc
    11521 gctcaaggtg gacgacgtgc gcaaggcgag gtgcctcgaa gccgcctatc aggccggcgt
    11581 gcgcgtgacg gccccggcgc ccggcgaatt ggagtttgcg acgaccggcg gcccggaggt
    11641 ggtggtgacg cggaaagcag gcacgctctt cggcctggat gagaaaacgt ttgcgccggt
    11701 caaggatgaa gagacgcaga tgtgcgcggg aatggttctc tcggtgatct atcccggtca
    11761 tatcgcagca gtgcgcaaac cggatggctc ctgggcgatc gtcttctaat tccatcgttc
    11821 ttctgctcgt cctggatccg ttaggcgtgg tttcgaatta caagctgggc tcgaatcgat
    11881 taggattacg agctgaagca ggatgatggg cagattaagg gagagattga cgccccccgc
    11941 tgcgactgtt tattcccccg tgccgaccga gatcaaactc ttcagtcgcg aatggtgctc
    12001 ctggtgcatc gacgccaagg attatcttct gcaacatggc taccagttca ccgagatcga
    12061 tgttggccgc gatcgcgagg cttacgagga gatgagggag ctgtccgacc agacctatgt
    12121 cccgactctc gtggccgggg gtgaagtgct tgcgaatttc gataccgatc agctcgcgaa
    12181 attcctccgc gtgcacgcca tcgtccccta aatgctgttt gggcttttca tcacccttgg
    12241 cgtcgccgtg ctcagcgtcg ccctgcgcag ctaccaatcc tcggtctcgc aaaagatcgg
    12301 cgccttcggc attctcaccg cctcctttct cgccatctat tatattaccg gcaactgggt
    12361 gctcgggctc ctcagcgcgc tcagctggct ctttcttccc tggctggaaa ttctcactcg
    12421 catccgcgcc ctccggctcc cgcgcgagaa aacgctccgc ccgaaaagcc cgccctcgcc
    12481 ggaggttttc ccgacgctcg atgagatcac cgcacaaatt gagagtgaag gcttcatcca
    12541 cgtgggcgac gccggctggg attgggagga ttacaagcaa ttcttccggc tcttttataa
    12601 ggaggtcgac cgtgcacagg cgacgatctg cctcaacgag cagcacgatc tttccttcta
    12661 ctacctgcgc atctcctcgc gggggcggga cggcacgatc tggacgacct ggaattatcc
    12721 cttgtcctac ggtttgaaat taaccccgca atttcggatc aaccggcaac ggccggacca
    12781 atcgttttgg cagctttacc atagtcacaa ggcttttctg cgcgaccacg cggtcgagac
    12841 caacgtgatc gatgcgctcg acgaagatca gattcagcag gagatcgaga acgatctgcg
    12901 cgaccagatc gcgcataaca tcaagaccgg cgtactcatg caaacgccag gcggcgagat
    12961 caagtattcc tggcgcggga tgatttatct ctggtgccag tttctgctcg atctcgtccg
    13021 cctctagcgg cggaaaatcc gaaaatcgaa tgtcgaaatc cgaaataaac gcggaaaatc
    13081 cgaaacccaa atacgactgc gtagcatcgt tttccctgca tccgaatttt ctgcattcgg
    13141 atttgtttcg gatttcgggc ttcggatttc ggatttaagc cggaaggctc ttcgatgaac
    13201 gaaaccgccc cgcgtattca gactgaagtg caggttatgt tttttgatac cgactgcgcg
    13261 gccgtcgtgc acaacatcgc ctacctgcgt ttcatcgaaa ccgcgcgtac cctgctggcc
    13321 gagcaacttg ggatgaaatt gaccgagatg gccgagaatc ggcgttatcc cgtcgtcgtg
    13381 cggacggaaa tcgactaccg tcgcgccgcg aagctgggcg atcgcttgcg catcgacggc
    13441 tggctggacc ggctggagcg cgtccgtttc tggtgcgtct ttcaaatccg gcggatcgag
    13501 gatgacgcgc tcatcgtcga ttgccggcag actctggccc tcatccagct gcccgaggcg
    13561 aagttgctcc gcttgccgga ggaatgggag cgttttcgcg cgccggaggc agccgatgtt
    13621 cctcgctgaa tccagcggca gatttttcgg ccgaaagccg tagcccaacg aagatgtcca
    13681 caggcaatcc cgccctcacg ctcgacgaag ctttcctctc cccgcgtgct ctggagccgc
    13741 cgccgatccg gcgcgcgctt cttgttttcc tcctctgcct ggccgcgctt ctccatgtcg
    13801 cgacgagcgg ctggggcgac ctctacaacg aaaccgacgg ccagtacgcc ggcgccgcgc
    13861 gtgaaatgct gcagtcgcac caatacctca cgccgacgaa tgacggcatc ccgcggctgc
    13921 aaaaaccgcc gttgctctac tgggcgatcg cgctctctct gaaagcgttt ggcctaacga
    13981 ccgcggctgt tcgcctgccg atcgccctcg gcacggtcgt cgccatcgcg ctcacttttc
    14041 tcattggcga ccgcctcggc ggcccgtggc ggggctttct cgccgggctg atccatctgt
    14101 gtgcctgcgg cacgtttctc cttggccgca tcgtcatgcc ggagccggtt ttcagcgcct
    14161 tgatcgccgg ttcgatctac tgcgcgcttc gcggctacga gcgacgccag ggccggcgcg
    14221 gttggttcat cggtttctgg ttttgcgtcg cgctcgcctg catgacgaaa agcctgcacg
    14281 ggctgcttta cccggcggcg atcctcgggt tgcttgcctg cttttatcgc gaagcgcggt
    14341 tgcggtttcg cgaattgctg cgctgggacg ggattctcgt ttttctactc ctcgtcgcgc
    14401 cgtggcatct ctggctggag tggaagcatc ccggttttct cagccaattc accgcgcagg
    14461 aatggctcgt ccatctcgcc ggcaaggcgg accagggtca tagctacgac aacgtgccgc
    14521 gtcatgtctt tctcgcactg cacctggcgt ggtggttccc ctggacggtc gcgatcctgc
    14581 ccggggtgtt ctgcgcgtgg cgccgcgttt tccgtccccg cgaaatcgaa tttgccgacg
    14641 ccctcccgct ctgttggatg gcggtggttt tcatcccgct cttcttcatc ggccaacggc
    14701 aggattacta ctcgatgagc atgtggggcg gcttcgccat ctttgccgcg actgcgtggg
    14761 aacgaatgcc gcgctggacc agagtcgtcg gcgtctcggg tgtgttggtg attggcctcg
    14821 tcgccggctt cgtcgcgtgg cgcttgccgg aactcttgtc cggcgtaagc ggccactggg
    14881 gtgtgaccgc cgcgcgctcc accgcctggc gcaccttgct ggatatcccg acttccacct
    14941 ggctgggttt tcgctcgatg ttcgtcgtga tcgggctcgc gctcgttctt ttctcggcaa
    15001 tcgcactctg gttcgtctgg cgggagcgac cgcgcctcgc cctcatggcg ctcgccgccg
    15061 cgatggtgcc gattggcttc agcatgatgg acggggtggc acggatggcg ccgtatttct
    15121 cactcgccga agcggctaat ttcatccagg agcgcatcgc gcctaacgac aagatcgtct
    15181 acgagggacc aatgcatgtc ggcagcagcc tgctctttta tctgggccga aaattctacc
    15241 tcgtgaacca ggatccggct tccgagcccg gggcggcgct cggaccgacc ccggatattt
    15301 ttctcgacga aaagaccgtc ctggaggcgt ggaagggcgc cgatcgtttc tatctcctgg
    15361 tcgagcggga gcggttaccg cattggcatg cgttgctccg cgcgaacggc gcggcgccgg
    15421 aagagttgac cacctgcggc acgaccgtgc tcctgagcaa tcatcgttag gcaaggcccc
    15481 gctcgcagga gcgcgcgagg agaccgctac ttctgcgtga gcggcgcgcg cacctccggc
    15541 tggagcaggc ggccccagag cgcgctgtag ggatggaccc gcgtcacacg ctctccgtcg
    15601 cgcgccagcg gccgatgctc gttcgcccac tcgaaaatcg agccttcgag attctgcacc
    15661 cgcgtgtaac cggcggcgcg cagccgttgc gccagcgcgc cggagcgata accgaccgcg
    15721 caataagtca cgatcggcgc atctcttgcg accttccccg ccgcggcgct cgcatccgct
    15781 tgcggatcga cctgccgcgc gcccggcaaa tggctcactt tccactcggc cggcgtccgc
    15841 acatcgagca gcacgggcgc gggccgtttc ttgtcggcca accaatcggc cagttgctgc
    15901 gtggtgatcc agttttcctc gccgaatctg ccgcgcagcg agcgcttgag aagaaaccaa
    15961 tcgattcccc gacaggaaag caatccgagc gcgcagagca gcaccgccgc gaacccgagc
    16021 gcaagccagg cattggctgc tccggaaagg cgtcgcggtc ttaaaatgca atccatttga
    16081 aaaagccgcg aggccagcta ccttgcccgc tcattttcgg actataaccc ggagcaattt
    16141 atgtcgcagc atcgcagtct taagggcgcc agcaccatcg cggccaagcg caatgttttg
    16201 aaacgtttcg agcgggtcga attgctcaaa aagcgcggcc agtggaaaga gacgagcaaa
    16261 gtcatcggtc tgccgaagac caagccagat ctctaggaga ggttggtagt taagagttga
    16321 tggttgagag caggaattct ttctctcaac tctcatgcgc cttaatcgct ttctcgccgc
    16381 cgccgggctc ggctcgcgtc gccattgtga cgagctgatt gcggaaggcc gcgtcaccgt
    16441 caacggccag ccgtgcacga atttcagcac gcaagtcgaa gcgagcgacc acgtcaaggt
    16501 caaccaccgg ctcgttaggc cggcggaaac gctgaccatc gcgctgcaca agccggctgg
    16561 tttcgtttcc acccggcgcg atccgaacgc gaccgacacg atctacgatt tgttgcccgc
    16621 gaaattttcc cgcctcttca acgtcggccg gctcgacgcc cagaccgaag ggctcctcct
    16681 gctcacgagt gacggggatc tcgcccagca cctcacccat ccgagctaca aggtggagaa
    16741 ggaatatgag gttacgctcg atcgacgctg ggatccaaaa cgcgggcaga aattgctcga
    16801 cggcgttttt ctcgacggcc ggcgcgcccg acttgcgcgg ctgcaaccga tcggcccgac
    16861 ccggctgcgc gtcgtcctgc aacagggcat gaaccggcaa attcgccgca tgttttacga
    16921 gatcggctac gaagtggagc ggctcgtccg tacccgcatc ggccacctgc ggctcggcga
    16981 gctgccgcgc ggcagctggc ggcccctaac gaaaagcgaa ctggccaggg ttcgttctca
    17041 gcctttgtcg ggcgggcgct ccgccggccc ggttttttga ccctcgatcc tgggcaggcg
    17101 gagcgcccgc ccgacattca ctccagctcg tcgcgcaaaa ttgcgagggg cggcgtgtta
    17161 ccaaggccgt agctggtcag caggccgatc gcgacggtta gggcgctcac gatcgaaccc
    17221 gcgatcacca ccggcaggag cgccggcgta taggccagct tgaaggtgaa aacggccagc
    17281 gcccagctcg cgacgagcgc gagcacgatc ccggtcgcac tcgcgagcag gcccaaaaac
    17341 aaatattccg tgccaagaat tttccagatc tgccagcgcg aggcgcccag cgtgcgcagg
    17401 agcacgctct ctttcagccg ctggtaacgt ccgctccaga tcgtgctccc gagcacgatc
    17461 aacccggtgc cgacggtaaa gagcgacatg atacggatga cgagcgcgac cttggtcagg
    17521 atcgaatcca ccgtttgaat aaccgaggtg agatcgagcg cggaaacgtt cgggaacctg
    17581 gccacgaccg cgttttgcag ccgggccgag gccgccggcg tcggaacgcg actgacgagg
    17641 acgttaaaag tcggggcctt ttccagcacg ccggtgggaa aaacgatgaa gaaattggtt
    17701 tgaaaacgtt tccaatcgac ctcgcgcaaa cttgccaccc gtgccttgat cggcacgccc
    17761 tgcacatcga aggctagggc atcaccgatc gttaggccaa gatccttcgc gatatcctgc
    17821 tcgacggaga tcggcacggt gtcgcccgga tgataatcga cccggccgac ccactttccg
    17881 gcggtgatct tttccgtctc gctcaactgg gcgcggtaag tcgagcggta ttcgcgttcc
    17941 agctcccatt ccggcgcctt gttttgcggg tccttcagca tctcggccgc cttgcggcgt
    18001 ttcacctcga gcagacgcat ggtcacgatc ggggcttgct ggaggacggg gaagcccaat
    18061 tgcctgacga gtccggcgac cgggccggtc tggcctggct ggatgtcgaa aaggaagatg
    18121 ttgggctggt cgcgcgcccc gatcgagcgg aactgggtca ggagcacgtc gcgcgtcagg
    18181 taaatggtaa ggagcaggaa agtgcccagg cctaacgaaa gggtgagaag cagggtgcga
    18241 ttgttggggc gatacagatt agccagcccc tggcggagaa cgaagctcca gcgctgcggc
    18301 aaaagtttgc gcacgagaaa aatcagcagc ttcgccacgc cgatgaggac cgccacggct
    18361 gtggccagac cggcggcgag aaataaccca tcggtccagg cttccgcctg cgagatcgca
    18421 aacgcggtca ggctggcggc gatcgcgaaa taaacgagcc agatcacgag gtcgcgcgcg
    18481 accggggcgg cgccctcttc gaccgacgag cggagagcga tgagcggcga cacccggcgg
    18541 aaacggagga gcggcgggag tgcaaagagg aggcaaacgc ccaatccgac ggccatgccg
    18601 cgcaggacag gtagccacgc gatcgcggtt ggcaggacga acggcagata tttctggagc
    18661 gcgccgggaa gagtttggtg aatcgttagg ccaagcgccg ctcccgtcag gacgccgacg
    18721 aaccccatcg ccatcgcctg aaggagatag acgatgacgg tggtgcggcc ggccgcgccc
    18781 agacaacgca ggatggcggc cgtccgcacc ttttgttgga ggtgtgcctg gatcgcgctc
    18841 gccaccccga ccgcgccgag caggagcgag atgaagccga cgagattgag gaagcggtag
    18901 aggttgtcga gcgattcgcc cagatccttc ttccgtttcg cgaccgtgtc gtattcgagt
    18961 ccgaaatgct cgattcgcgg cgcgagtttt ttgacttcgc ctggcacgtc gacgttcggc
    19021 gcgaacttca cgaagttcag gtagcgcgcg aggctgccgg gcttgagcag gttggtttgc
    19081 gcgagatttt gcagcggaat gtaaacccgc ggcgcgaaac tcgcggcggg cgaggcttcg
    19141 cccggcatct tggtcagcgc gccggcgatc gtgaaggtcg cttcgccgat cttgatcggg
    19201 tcgccgggtt tcgcccggaa ttggaagagc agactttcct cggggacggc ctggcctccg
    19261 gtccggaaag tcttcgccgc cgtggccggt gcggtttcca tcctcccgta gaagggaaaa
    19321 gctccgccca gggcgcggac gtggacgagg cgggtgccac cggctttcgg gaagagcgcc
    19381 atcgtgttga agcgcacttc gcgcgcctgg atttcgccta acgaatggag catctgttcc
    19441 tgctcggggg tgaagggccg cgagctttcc accaccagat ccgccccgag gagcgaccgc
    19501 gcctggtcgt caatcgcctg ggccaggctg gcgcggaaca aaccgatcgc gattaaagcc
    19561 gctacgccta acgagatcga aatggaaaaa aggaggaggc gcgagcgact cgcgcggctg
    19621 tcgcgccacg ccatgcgaaa aacgaatccg atgttctcgt tcgcgtggcg cattattgct
    19681 tgaagggacg tgcttccgcc cgtccggttt ctcccgtccg cgtcgcgatt ccaaacggga
    19741 cgtgcggaag cacgtccctc cgaaagaaac cgctcatccg accatcgctc cgttccgcaa
    19801 gcgaatcgtc cgctctgtca aacgcgcgac ctcgggatcg tgggtcacga ggacgagtgc
    19861 ggtgcccgcg ctgcgattca attcgagcat gatctcgatc acgttctggc tcgtctccgc
    19921 gtcgaggttg ccggtcggtt cgtcggcgaa caataccttc ggtcggttga taaacgcccg
    19981 cgccagggcg acgcgctgct gttcgccgcc ggaaagttgc gtcgaataat gatcgctccg
    20041 gtcgccgagt ccgacgcgtt gcaggaggtc gagggcgagt gcccgtgcgc tgcgatcgcc
    20101 gcgcagctcc atcggaacgg tcacattctc gagcgcggtg agcgtgggaa tgagctggaa
    20161 attttgaaaa acaaaaccga catgctcgtt ccggacgcgg gcgcgttcgt cctcgctgat
    20221 ttcggagagc gcgatgccgt tgagcagcac cgagccggaa gtgggaagat cgagccccgc
    20281 cgccagaccg aggagcgtcg ttttcccgct acccgaaggg ccgaggatcg agcaggtcgc
    20341 gccctgctcg agcgtgaacg agatatcgcg caaaacggtc aggcgacccg cgccggtggc
    20401 gtagcttttc gttaggtcgc ggacctcgag aatcgggcac gagttcattg acgggtcagg
    20461 gtggcgcgtc ttgcttacgc gatggtgata cggcgcgcat gaggaaattg atgcgccgcg
    20521 gttttgttgt tctcttttgc gcactcgctt gcgcctcgcc cgctcgcggt ggcacgattc
    20581 tctttctcgg cgacagcctg accgccgggc ttggagtcga acagggacag gctttccccg
    20641 cgctgatcca ggaaaagatt cgcgaaaaga atctgccctt tgacgtgatc aacgccggga
    20701 tcagcggcga cacgacggcc ggcggactgg cgcggctcga ctgggtgttg cagaaaaaaa
    20761 tcgacgttct cgtcctcgca ctcggggcaa atgacggatt gcgcgggctg cccgtcgcgc
    20821 agatgaaagc gaatctccag gccatcatcg accgggtgaa agcgaagaac ccggcggtga
    20881 aaatcgtcat cgccgggatg cggatgccgc cgaatctggg cggcgcttac agcgacgcgt
    20941 tccgggaagt ttacggcgac cttgcgcgtg agaaccacgc ggcgctcatt ccgtttcttc
    21001 tggagggcgt cggcggccac gatgatttga atcaggcgga tcatattcac ccgactgccg
    21061 ccgggcataa agtggtggcg gaaaacgttt ggcgggtgct gggcccgctg cttgggaacg
    21121 gcggatcgta atgcggctcg acggctgcaa cgcgctgata accggcgctt cggcggggat
    21181 agggcgtgag ttcgcgcggc aactgggcga ccgcgccgcc acgctcgtgc ttgtcgcacg
    21241 gcgccaggcg cgcctcgagg aattgcgcgc ggagctgacg gagcggcatc cgcaattgcg
    21301 cgtcgaaatc cggcccaccg atttgtcgca acacgaagcg gtcgacgcgc tgctcg
//
LOCUS       DATMNS010000023         3973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_750296, whole genome shotgun sequence.
ACCESSION   DATMNS010000023 DATMNS010000000
VERSION     DATMNS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750296"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(201..1235)
                     /locus_tag="VNW28_00990"
     CDS             complement(201..1235)
                     /locus_tag="VNW28_00990"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-enoyl thioester reductase domain-containing
                     protein"
                     /protein_id="HXA08523.1"
                     /translation="MSGSIKAIVYHAHGKPEEVLRLEEQPLPQPAPDEALVRMLAAPI
                     NPADLNAIEGKYPIRPELPATPGMEGAGVVVEIGARVRDLAVGTRVILPHNLGTWREA
                     CVVAAEKLVAVPDEIEPAQAAMLKINPLTAWRMLHDFVALQPGDWVIQNAANSAAGRA
                     VIHLARELGYRTINVVRRTELIEELRAEGGDVVLLDDDNLRAAAAGATAGAPIRLALN
                     AVGGESALRLAKIVAPEATIVTYGAMSLRPLTLPNGLLIFKNLHFTGFWINKWYEQAT
                     PAARNEAFARIFAFTRRGLLQTKIEHTYPLGEFRTAVARAAEGKRGGKIIFCMESQNM
                     GTAVARRRAA"
     gene            1239..2147
                     /locus_tag="VNW28_00995"
     CDS             1239..2147
                     /locus_tag="VNW28_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXA08524.1"
                     /translation="MAQHPTANTPAGNLRSGARLALLGLVANFVLAGVKITAGFLGNS
                     FVLIADGIESGLDVAGSAIIWGGLKVAALPPDATHPYGHGKAEPVAAVIVSLGVIAAA
                     FGLAVQSAQAIFQPHHAPRPFTLAVLIVVVIVKECLYRAVIRIGRNVESTAVQTDAWH
                     HRTDALTSVAAFIGISIALLGGPAWQSADAWAALFACALIGANGYRLLNPALHEIMDT
                     APRGGIVSAIRAAAASVHGVRHVEKCLVRKMGLEFYVDLHVGVDETISVRAGHSIAHE
                     VKDAIRRTDSRIADVLVHVEPAGPAR"
     gene            complement(2144..2788)
                     /locus_tag="VNW28_01000"
     CDS             complement(2144..2788)
                     /locus_tag="VNW28_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08525.1"
                     /translation="MIAVTFALPSESSDFRRLLSHQGHEVAVLHTGVGEKSCRSRLGP
                     LLDGKPFDLLISSGFAGGIDPSLGVGDLLLAENFSDPQLVAQARALLVGQVARLVTAD
                     RVVESAGERQRLAQEHNAAAVDMETQWIARACAERKLPMLSLRVISDTAAAPFPAPPA
                     VLFDLERQRTDGTKVATHFFRHPFHLVRLIRFARQIATARENLGAALYQLIATL"
     gene            complement(2800..3453)
                     /locus_tag="VNW28_01005"
     CDS             complement(2800..3453)
                     /locus_tag="VNW28_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08526.1"
                     /translation="MTPEKFFDYLEGKLPPAEKERLERALIADPELQRQFVSARQIHR
                     SMQRPFDESAATTRAGSRGRQLAAAFAVLVAMNVALGLVYIFRANQPPRELQKARAEA
                     LRQQLQSSVEKSAVAAFPPPNIGMERVALTTSREKRDEVAKTIIDAATKAGGSGTRGL
                     PNDSGGSVLVIVPAAAEPAFWKVLAALGAPPPAPLAASPAPLPNESVRLEVVLSSPP"
     gene            complement(3506..>3973)
                     /locus_tag="VNW28_01010"
     CDS             complement(3506..>3973)
                     /locus_tag="VNW28_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXA08527.1"
                     /translation="PRSNQREDFLPRINRISRMEKMIHEELSRAIIGSAMEVLNELKP
                     GLDEKLYERAMIIELRRCGRTATVQPAFPVSYRGELIGNLVPDLIVDDLVIVDPKVVS
                     IFTDTHIAQMLGYLKITGLKLALLLNFKNASLSWKRVVNQSLRENSARPDLHA"
BASE COUNT          704 a         1234 c         1249 g          786 t
ORIGIN      
        1 ggcgatttat taagccgctc cttcgcccaa tcctgggcgg tgacggttcc gctgcctgac
       61 gagatgaaca ataagccgag ggcaagagcg cgaagtttca tccgttaagg aacgccgctg
      121 tcgcgccgcg gcaactgctc agactgttca taacgtgaga ggggcctgag ggccccgatt
      181 ggttgggtgt tgcctggaat ttacgctgca cgtcgccgcg caacggcggt tcccatattt
      241 tgcgactcca tgcaaaagat gattttcccg ccgcgctttc cctccgccgc gcgggcgacg
      301 gccgttcgga attcccctaa cggataggtg tgttcgattt ttgtctggag caggccgcgc
      361 cgcgtaaatg caaagatccg ggcgaaggct tcattccgcg ctgcgggcgt cgcctgttcg
      421 taccacttgt tgatccaaaa accggtaaaa tgcagattct tgaaaatgag caatccgtta
      481 ggcaaagtga gcggtcgcag actcatcgcg ccgtaggtca cgatggtcgc ttccggtgcg
      541 acgatcttcg cgaggcggag cgcgctctct ccgcccactg cattcagggc cagccgaatc
      601 ggcgcgccgg ccgtcgcgcc ggccgcggcc gcgcgcaagt tgtcgtcgtc gagcagcacc
      661 acatcgccgc cttccgcgcg cagttcctcg atcagctccg ttcgccgcac gacgttaatc
      721 gttcgataac ccagttcgcg cgcgaggtgg atgacggccc ggccggcggc ggagttggcc
      781 gcgttttgaa tcacccaatc gcccggctgc agtgcgacga agtcatgcaa catccgccag
      841 gcggtgagcg gattgatttt caacatcgcc gcctgtgccg gctcgatctc gtcaggcaca
      901 gcgaccagct tttccgcggc cacgacgcag gcttcccgcc aggtgccgag gttgtgtggg
      961 aggatcacgc gagtgccaac cgcaagatcc cggacgcgtg cgccgatctc gaccaccacg
     1021 cccgcacctt ccattccggg cgtggccggc agttccgggc ggatcggata tttgccctcg
     1081 atcgcgttca ggtctgcggg attgatcggg gcggccagca tccggacgag cgcctcatcg
     1141 ggtgcgggtt gcgggagcgg ctgttcctcc aaacgcaaaa cctcttccgg ttttccgtgc
     1201 gcgtggtaaa cgatcgcctt tatactcccg ctcattcgat ggcccagcat cctaccgcga
     1261 acactccggc cggcaatctc cgatcgggcg cccgcctcgc ccttctcggg ctggtcgcaa
     1321 acttcgtcct cgcgggcgtg aaaattaccg ccgggttcct cggcaactcc tttgtcctga
     1381 tcgccgacgg gatcgagtcg gggctcgacg tcgcgggttc agcgatcatc tggggcgggt
     1441 tgaaggtggc cgcgcttccg cctgatgcga cccatcccta cggtcacggc aaggccgaac
     1501 cagtcgcggc cgtcatcgtg tcgttaggcg tgatcgccgc cgctttcggc ctcgctgtgc
     1561 aaagcgcgca ggcgattttt caaccgcatc acgcgccccg accattcacg ctcgccgtcc
     1621 tcatcgtggt cgtgatcgtg aaggaatgcc tctaccgcgc ggtcattcga atcggccgga
     1681 atgtcgagag caccgcggtc caaaccgacg cctggcatca tcggaccgac gcgctcactt
     1741 cggtcgcggc tttcattggc atctccatcg cgctgctcgg cgggccggcc tggcagagtg
     1801 ccgacgcgtg ggcggcgctt ttcgcctgtg ccttgatcgg agccaacggc tatcggctgc
     1861 tcaatcccgc gctgcacgaa atcatggata ccgctccgcg gggcgggatc gtgtccgcga
     1921 ttcgagctgc ggcggcatcg gttcacggcg tgcggcacgt cgagaagtgt ctggtccgga
     1981 agatggggct ggagttttac gtcgatctcc atgtcggagt ggatgaaacc atctccgtcc
     2041 gcgccggcca ttctattgcg cacgaggtaa aggacgcgat tcgccggacc gattcccgaa
     2101 tcgccgacgt cctcgtgcat gtcgagccgg cgggtccggc gcgctaaagg gtggcgatca
     2161 gctggtaaag cgcggcgccc agattctcgc gggcggtggc gatctggcgc gcgaaacgga
     2221 tcagcctaac gagatggaac ggatgacgga aaaaatgcgt ggcgaccttt gttccgtcgg
     2281 tcctttgccg ctcgagatcg aagagcacgg ccggcggagc aggaaatggc gccgccgccg
     2341 tgtcactgat cacgcgcagg gaaagcatgg gcagctttct ttcggcgcaa gcgcgggcga
     2401 tccattgtgt ctccatgtcg accgccgcgg cgttgtgctc ctgcgccagg cgctggcgct
     2461 ccccggcgct ttcgaccacc cgatcggcgg tcacgagccg ggcgacctgg ccgacaagaa
     2521 gagcgcgggc ctgggcgacc aactgcggat ccgagaagtt ttccgcgagg agaagatctc
     2581 ccacgcctaa cgaggggtcg atcccgccgg cgaagccgct actgatcaaa aggtcaaagg
     2641 gtttgccgtc gagcaacggg ccgagacgtg agcggcagct cttttccccg accccagtgt
     2701 gcaagacagc gacctcatgt ccttgatgac tgaggaggcg ccggaagtcg ctgctttcgc
     2761 tcgggagcgc aaaggtaacc gcgatcatgg gcgcctcgtt cagggcgggc tggagaggac
     2821 gacttcgagc cgaacggact cgttaggtaa aggcgccggg cttgcggcga ggggagcggg
     2881 gggaggagcc cccagtgccg cgagcacttt ccagaaggct ggttccgcgg ctgcggggac
     2941 aatgacgaga acgctcccgc cgctgtcgtt aggcaaaccc ctggttccgg aaccgccggc
     3001 tttggtcgcg gcgtcgatga tcgtttttgc cacctcgtcc cgcttctccc gcgacgtggt
     3061 cagcgcgacc cgttccatcc cgatatttgg cggcgggaac gcggcgaccg ccgatttctc
     3121 aaccgagctt tgcaattgct gacgaagcgc ttctgcgcgc gccttctgca gctcccgagg
     3181 cggctggttc gcgcgaaaaa tgtagaccaa gccgagcgcg acgttcatcg ccacgagcac
     3241 ggcgaaagcc gcggcgagtt gtcgaccgcg cgaaccggcg cgcgtggtgg cggccgattc
     3301 gtcgaagggt ctctgcatgc ttcgatgaat ctgccgcgca ctaacgaatt ggcgttgcag
     3361 ctccgggtcg gcaatgagcg cccgttcgag gcgttccttc tccgccggcg gcaactttcc
     3421 ttccaggtaa tcgaagaatt tttcgggagt catggcgatt cggaattact cgaccgcgga
     3481 ttacgcggat ttctcggata aagaatcatg catgcagatc gggccgggcg gaattttcgc
     3541 ggagcgattg attgacgacc cgcttccagc tcagactcgc gtttttgaaa tttagcaaga
     3601 gggccagttt caggccggtg atcttgaggt agccgagcat ctgcgcgata tgcgtgtcgg
     3661 tgaagatcga caccaccttt ggatcgacga tcaccagatc gtcaacaatc aagtcgggga
     3721 ccaagttccc gattaactcg ccgcggtaag aaactggaaa ggcaggttgc acggttgcgg
     3781 tgcgaccaca ccttcgtagc tcgatgatca tcgcgcgctc gtaaagcttc tcatcgagtc
     3841 cgggcttcag ttcgttcaag acctccatcg cgctgccaat aatggcccgg ctcaactctt
     3901 cgtggatcat cttttccatc cgtgaaatcc gattaatccg cggtaaaaaa tcctctcttt
     3961 gattgcttcg agg
//
LOCUS       DATMNS010000024         5327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4687983, whole genome shotgun sequence.
ACCESSION   DATMNS010000024 DATMNS010000000
VERSION     DATMNS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4687983"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VNW28_01015"
     CDS             <1..231
                     /locus_tag="VNW28_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXA08528.1"
                     /translation="AHPWLAPREILEIATVNGARALHQEETLGKIRAGAQADLIALPI
                     DNNRGDIFEKIVAWSDPVSWLLVAGRAPSDNL"
     gene            408..1778
                     /locus_tag="VNW28_01020"
     CDS             408..1778
                     /locus_tag="VNW28_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXA08529.1"
                     /translation="MAKIIIIDDEPAMVEVIVTLCRDKGHQVFPFSSAAKAVEQMATI
                     EPQIVITDVKMETISGFDVLRHVKEHHKQTLVILITAYASVDTAIEAMKMGAHGYVPK
                     PFKIDELQMTVQRALEFQAARQENTYLRRELKNKYNFENIIGSSRKMQNVYTLISKVA
                     DTDSTVLIQGESGTGKELVARGLHFNSNRQQHPFVAINCSALPENLLESELFGHKKGS
                     FTGAVTDKRGLFEEANLGTIFLDEVNSMATSLQTKLLRVLQEREVRRVGDNRSVPVNV
                     RVVGATNEPLQPKIRDGSFREDLYYRLAVIPVEIPALRERLEDVPLLVNHFLQKQANQ
                     TGGEPKKIDPKAVDLLCRYDYPGNVRELENAIERACALCENDLIVPDDLPPQIVEFAS
                     EEKAKKARATLPVGKSLDEFIQEQEREYIEATLARFKGAREKAANVLGISMATLYRKL
                     EVKGKK"
     gene            2026..4287
                     /gene="glgX"
                     /locus_tag="VNW28_01025"
     CDS             2026..4287
                     /gene="glgX"
                     /locus_tag="VNW28_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HXA08530.1"
                     /translation="MTPIKVWLGFPYPLGATWLGNGVNFALFSETAASVDLCLFDSPE
                     ARQENMRIPMTEHTDQVWHIFLPDARPGQLYGYRVTGPYEPERGLRFNSAKLLIDPYA
                     KAIAGQVDWSDEMSGYVVGNPGEDLTPDFRDDAWGIPKSVVIENTCDWGDDRAPRTPL
                     HSSIIYELHTRGFTQLNEKVPEEIRGTYAGVGSAASIEYLKELGVTAVELLPVHAYIN
                     DQALVDRGLTNYWGYNSIGFFAPECKYSSSGVTGGQVTEFKAMVRNLHAAGLEVILDV
                     VYNHTAEGNHLGPTLSFRGIDNPAYYRLTPEDPRFYKDFTGTGNTFNLLHPRTLQLVM
                     DSLRYWVNDMHVDGFRFDLASTLARDANGVNKLHAFFEIIHQDPVISQVKLIAEPWDV
                     GEGGYQVGNFPILWAEWNGKYRDTVRSFWKGDEGKTGELAYRLAGSPDLYQHNGRRPY
                     ASINFITAHDGFTLNDLVSYNDKHNEANGEENRDGDNNNQSWNCGVEGPTNDPKIKQL
                     RLQQRRNFLTTLLLSQGVPMLTAGDEWARTQKGNNNAYCQDNEISWLNWNLDDEQKAL
                     LEFTKGLIRLRREHPVFRRPKFFQGRRIRGSEIRDVMWFNPGGAEMTEEEWNSPSVRC
                     LGMLLSGDTIDVQNFEGEPIRDNTFLLLINAHHEPLQFDLPGQKRVTWQLLLDTASAD
                     GFCQEPRHCLSGDELEIKERASMLLKLTTRSTTRALHESWKKRQFELPHDGEAPKEAA
                     TPEPKEDPARAEE"
     gene            4324..4785
                     /locus_tag="VNW28_01030"
     CDS             4324..4785
                     /locus_tag="VNW28_01030"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HXA08531.1"
                     /translation="MTPTSLIVVADRGGVKAYRVNETPSRGASLQLVQAFDLTDSHGR
                     YDDKLTDQAGRFPVSDGHSRHANSIAERTALETENERRICKQLAEHIAEVVKRETAAG
                     WSFAAPASIHATIAELLPAPIRDRVVEYVKSDLLKIESSKLAQHFRSLQSL"
     gene            4806..5138
                     /locus_tag="VNW28_01035"
     CDS             4806..5138
                     /locus_tag="VNW28_01035"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HXA08532.1"
                     /translation="MQLPVEVSYRGVEKSDQLDELIRNKAARLDRFCDHISRCDVVIE
                     HPNRAQHSGNPFRVRIDVTVPPGHELVADEKQAEHEMHEPLSKVINDAFKTMERQLKA
                     LVEKQRRE"
BASE COUNT         1150 a         1624 c         1573 g          980 t
ORIGIN      
        1 gcgcatccgt ggctcgcacc acgagaaatt ttggaaatcg cgaccgtcaa cggcgcgcgc
       61 gcgctccatc aagaggagac actgggcaaa atccgcgccg gcgctcaggc cgatctgatc
      121 gcgctgccga ttgataacaa ccgcggcgac atcttcgaaa agattgtcgc ctggagcgac
      181 ccggtctcat ggctgctcgt cgccggtcgc gccccgagcg ataatctgta gccggcggcg
      241 ctgattcttt cctccgcggt ttgcaatttt cgcgaaggac aaaactgcga ttcgccagcc
      301 ggccacggcc gaccgggggg gcgccccggt tacggcaagc catcctttgc gattctcatc
      361 tttgactttc cttttctcat ccgtgcgata ttctccgccc tgagaatatg gctaagatca
      421 tcatcataga cgacgaaccg gcgatggtgg aggtgattgt gacgctctgc cgcgacaagg
      481 ggcatcaggt tttcccgttt agttccgccg ccaaggcggt cgagcagatg gccacgatcg
      541 agccgcagat cgtcatcacc gacgtgaaga tggagacgat cagcgggttc gacgtgctgc
      601 gccatgtgaa agagcatcac aagcagacgc tcgtgatcct gatcaccgct tacgcctcgg
      661 tcgataccgc gatcgaggcg atgaagatgg gcgcgcacgg ctacgtgccg aagccgttca
      721 agatcgacga attgcagatg acggtgcaac gcgcgctcga gtttcaagcg gcccgacagg
      781 aaaacacgta tctgcggagg gagctcaaga ataaatacaa ttttgagaac atcatcggct
      841 cgagccgcaa aatgcagaac gtttacacct tgatcagcaa ggtggccgac accgacagca
      901 cggtcttgat tcagggcgag agcggcacgg gcaaggagct ggtcgcgcgc gggttgcatt
      961 tcaacagcaa ccggcagcag catccgttcg tcgcgatcaa ttgcagcgca cttcccgaga
     1021 acctgctcga gtcagaattg ttcggccaca agaaaggctc gttcactggc gcggtgacgg
     1081 acaagcgcgg gcttttcgag gaagcaaacc tcgggacgat ttttctcgat gaggtgaact
     1141 cgatggcgac ttcgcttcag acgaaattgc tccgcgttct acaggagcgc gaggtgcggc
     1201 gcgtaggcga caaccggagc gtgccggtca acgtgcgcgt agtcggcgcg accaacgaac
     1261 cgctccagcc gaaaatccgc gatggttctt tccgggaaga tctttattat cgcctggcgg
     1321 tcattccggt cgagatcccg gcgttgcggg agcggctcga ggacgtgccg ctgctggtca
     1381 atcatttcct gcaaaaacag gcgaaccaga caggcggcga gccgaagaag atcgacccga
     1441 aggcggtcga tttgctctgc cgctatgatt accccggcaa cgtgcgcgag ctggagaatg
     1501 cgatcgagcg ggcgtgcgcc ttgtgcgaaa acgacttgat cgtgcccgac gatctgcccc
     1561 cgcagattgt cgagtttgcg agcgaggaga aggcgaagaa ggcgcgcgcg acgttacccg
     1621 tcggcaaatc gctcgacgaa ttcatccagg aacaagaacg cgagtatatc gaagcgacgc
     1681 tcgccaggtt taaaggcgcg cgcgaaaaag cggccaatgt cctcggcatc agcatggcga
     1741 cgctctaccg caaactcgag gtcaagggga aaaagtagcg gccgacttcg ccgacttttt
     1801 cctccccttc aatcccctgc ttgagcggct cggtcggaat gacgaagccc cgaatgacga
     1861 atgtcgaaaa caatgaccaa atgacgaatg atcaaagggg gccgctccga gtttcatcat
     1921 tcgtcattgg gcattgattc gtcattcgga attcgtcatt ccctcccgcg gtcgccgcga
     1981 cacttgcgtg cctcccatcc gataggataa acacgtccgc agttcatgac tccgattaaa
     2041 gtttggctcg gctttcccta tccacttggc gctacctggc tcgggaacgg cgtcaacttc
     2101 gctctctttt ccgagaccgc ggcctcggtc gatctttgcc tcttcgattc ccccgaggcg
     2161 cgccaggaaa acatgcgcat cccgatgacc gaacacaccg accaggtctg gcacatcttc
     2221 ctgcccgacg cgcggcccgg tcagctttac ggctatcgcg ttaccggacc gtacgaaccg
     2281 gaacgcgggt tgcgtttcaa cagcgccaag ctgcttatcg atccctacgc gaaagcgatc
     2341 gccggccagg tcgattggtc ggacgagatg tcgggctatg tcgtcggcaa tcccggggaa
     2401 gacttgacgc ccgacttccg cgacgacgcc tggggcatcc cgaagtccgt cgtaatcgag
     2461 aacacatgtg actggggcga cgacagggca ccgcgcacgc cgctgcacag ctcgatcatt
     2521 tacgagctgc acactagggg gttcacgcag cttaacgaga aggtgccgga ggaaattcgc
     2581 gggacctatg ctggtgtcgg gagcgcggcc tcgattgaat acctgaagga gcttggcgtg
     2641 accgcggtcg agctcctgcc ggttcacgcc tacatcaacg accaggcgct ggtcgatcgc
     2701 ggcctaacga actattgggg ctacaactcg atcgggttct ttgccccgga atgcaagtat
     2761 tcctcgagcg gggtgaccgg cggccaggtg accgaattca aagccatggt gcgcaacctc
     2821 cacgccgccg gcctcgaggt gatcctcgac gtcgtttaca atcacaccgc ggagggcaat
     2881 cacctcggcc cgacgctcag tttccgcggg atcgacaacc cggcctatta ccgcctcacg
     2941 ccggaagacc cgcgcttcta caaggatttc accggcacgg gcaacacctt caatcttctc
     3001 cacccgcgca ccctccagtt ggtgatggac agtctgcgct actgggtcaa cgacatgcat
     3061 gtcgacggtt tccgtttcga tctcgcctcc accctcgcgc gcgacgcgaa tggcgtgaac
     3121 aagttgcacg ccttttttga aatcattcac caggacccgg tcatctcgca ggtgaagttg
     3181 atcgccgagc cgtgggacgt gggcgaaggc ggctaccaag tcggcaattt cccgatcctt
     3241 tgggcggaat ggaacggcaa ataccgcgac acggtgcgca gcttctggaa aggcgacgag
     3301 ggcaagaccg gtgagctcgc ctaccgcctg gcaggaagtc cggatctcta tcagcacaat
     3361 ggccggcgcc cttacgcgag cattaatttt atcaccgcgc acgacggatt tacgctcaac
     3421 gatctcgtca gctacaacga caagcacaac gaagcgaacg gcgaagaaaa tcgcgacggc
     3481 gacaacaaca atcaatcgtg gaattgcggc gtggaaggcc cgaccaacga tcccaagatc
     3541 aaacagctcc ggttgcaaca gcggcgcaat ttcctcacca ccctgcttct ttcccagggc
     3601 gtgccgatgc tgaccgcggg ggacgaatgg gcgcgcacgc aaaagggcaa caacaacgcc
     3661 tattgccagg ataacgaaat cagctggctg aactggaacc tcgacgacga gcagaaggcg
     3721 ctgctcgagt tcaccaaggg actgattcgg ctgcggcgcg agcatccggt ttttcggcgc
     3781 ccgaaatttt tccagggccg acggattcgc ggctcggaga ttcgcgatgt gatgtggttc
     3841 aaccccggcg gtgccgagat gaccgaagag gaatggaatt caccctctgt ccgctgcctc
     3901 gggatgcttc tgagcggcga cacgatcgac gtccagaatt tcgagggcga gccgatccgc
     3961 gataacactt tcctcctgtt gatcaacgcg catcacgagc cgttgcagtt cgatttgccc
     4021 ggccagaaac gcgtcacctg gcaactcctg ctcgacaccg cctcggccga tggattttgc
     4081 caagagcccc gtcattgcct ttccggtgac gagctggaga tcaaggagcg cgccagcatg
     4141 ttgctcaagc tcacgactcg ctcgaccaca agggcgctgc acgagtcgtg gaaaaagcgg
     4201 cagttcgaat taccgcacga tggcgaagcc ccgaaagaag cggcgacgcc cgagcccaaa
     4261 gaagatccgg cgcgagccga agagtgagcg cgaaaagcgc gcggtgtgcg atcttctttt
     4321 gccatgacac cgacatcact tatcgttgtc gccgatcgcg gcggcgtcaa agcataccgg
     4381 gtcaacgaga cgccgagtcg cggcgccagc ttgcagttgg tgcaggcgtt tgacctcacc
     4441 gattcccacg ggcgttacga cgacaaactc accgaccagg ccggccgttt cccggtgagc
     4501 gacggccaca gccgccacgc caactcgatc gcggaacgca ccgcgctcga aaccgagaac
     4561 gagcggcgca tttgcaaaca actcgccgaa cacatcgccg aggtggtgaa acgcgagacc
     4621 gccgccggct ggtcgtttgc cgcgccggcc tcgattcacg ccacgattgc ggagttgctg
     4681 ccggcgccga tccgcgaccg cgtcgtcgag tacgtgaaat ccgatctctt gaaaatcgag
     4741 tcgtcgaagt tggcgcagca ctttcgctcg cttcaatcac tctgattgtt acagtaggtt
     4801 gggcgatgca attacccgtt gaagtttcat accggggggt cgagaaatcc gatcagctcg
     4861 acgaactcat ccgcaacaag gcggcgcgcc tcgatcgctt ttgcgatcac atcagccggt
     4921 gcgacgtcgt gatcgagcac ccgaatcgcg cccagcattc gggcaatccg ttccgggtgc
     4981 ggatcgacgt gaccgttccg cccggtcacg agctggtcgc ggatgagaaa caggccgagc
     5041 acgagatgca cgagccgctg agcaaggtga tcaacgacgc tttcaaaacg atggagcgcc
     5101 agctcaaggc gctcgtcgag aagcagcgcc gggagtaaga gcgatttggg gactgcgcaa
     5161 cgcccaacgt ccaacattta gcgttcaacg aagaggcggg ccgttgccct tgccgtctcc
     5221 gtagctggct gtagccgccg ccctgtgggc ggcgcggcca tgggccaggt cggcttgctg
     5281 aacattccag cctctctccc gcgcttcgcg cagcgaagcg gctacat
//
LOCUS       DATMNS010000025        10550 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4688429, whole genome shotgun sequence.
ACCESSION   DATMNS010000025 DATMNS010000000
VERSION     DATMNS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 10550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10550
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4688429"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..870
                     /locus_tag="VNW28_01040"
     CDS             136..870
                     /locus_tag="VNW28_01040"
                     /inference="COORDINATES: protein motif:HMM:NF021371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HXA08533.1"
                     /translation="MSTAFLTEEWRHLVMLNYAVEHKLLDPRVPRGLELDRFHSVAYL
                     SVVGFLFLKARVLGISFPFHRDFEEVNLRFYVRHWGPEGWRRGVVFVREIVPRRAIAT
                     LARRLYGEPYSAFPMMHAIERDGLELRCRYAWQRNGRREFVSASASGAAEPIASGSLE
                     EFITEHYWGYTARGEHCTQYQVEHPRWRVWRATAARFEADVASLYGPEFVAPLSAPPA
                     SAFIADGSPVVVRRGSPLASAAVATE"
     gene            complement(847..3720)
                     /locus_tag="VNW28_01045"
     CDS             complement(847..3720)
                     /locus_tag="VNW28_01045"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HXA08534.1"
                     /translation="MPNEFDTLQKFDAGHGRAAFFHSLPALEERGVGPISRLPVSIRI
                     VLESVLRNCDGQKVGRKDVETLANWNPKAPANEEIPFVVARIVLQDFTGVPLVVDLAA
                     MRSAVRRLGGDPKIIEPLVPVDLVVDHSVQVDFAGSAAALRLNMEMEFKRNRERYEFL
                     KWGQQAFKTFGLVPPGIGIVHQVNLEYLAKGVLQSGDVYYPDTLVGTDSHTTMINGLG
                     VVGWGVGGIEAEAGMLGQPVYFLTPEVVGVHIGGSLREGVTATDLALHITQMLRAQKV
                     VGKFVEFYGEGAASLPLPDRATVGNMSPEYGATMGYFPVDAESVNYLRATGRTDEQCR
                     AFEAYFRAQKMFGMPRQGEIDYSVDLELDLGDVQPSVAGPKRPQDRINLPELGSAFRS
                     LLEKPVKDGGYGKAGAELGRRHLVSLNGGPPKTEEMPSTDRPDQGIEPGDERNKIEMV
                     SNRPTPDKGSAIEAEDGEVFAQGQSCIGHGSVLIAAITSCTNTSNPSVMLAAGLLAKK
                     AVERGLTIDPSVKTSLAPGSRVVSDYLHKTGLQEYLDQLGFNIVGYGCTTCIGNSGPL
                     HPKIEQAINEFDLVAASVLSGNRNFEARVHQNIKANFLMSPPLVVAFALAGRVDLDLA
                     KDPLGKDKEGRAVFLKDLWPSLGEIRAVMQSALTPEIFRKLYRDFAGQNPKWNEISSS
                     TGEVYQWDEESDYIHDPPFFADFSMAPGRIEDIRGARPLGIFGDSVTTDHISPAGAIK
                     ASSPAGQYLVSRGIKPEDFNSYGSRRGNDLVMTRGTFANVRIKNLMVPGVEGGVTKFF
                     GHGQAATGEQMSIFDAALKYAEAKTPLIILAGQEYGTGSSRDWAAKGTRLLGVKAVVA
                     ASYERIHRSNLVGMGVLPLQFAEGTNAQSLGLNGSEVYSVTGLSESIQPGQNLTLEIT
                     RADGQEQAVPVKLRIDTPIEIDYYRHGGILPFVLRELLGGHGG"
     gene            complement(3803..4297)
                     /locus_tag="VNW28_01050"
     CDS             complement(3803..4297)
                     /locus_tag="VNW28_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXA08535.1"
                     /translation="MALAVGTKAPDFTLPTKNADGPRPVRLSENFGVKNTLLLFFPMA
                     FTGTCTTEMCGVGNGLREYGDLHCEVYGISGDNPFAQEAWAQKEGINVTLLSDYEHKV
                     AHSYGVAYESFLPQLHLGFAGVAKRSAFLIDRAGVIQYAEVHEDARELPDFDKIKARL
                     AELK"
     gene            complement(4426..5136)
                     /locus_tag="VNW28_01055"
     CDS             complement(4426..5136)
                     /locus_tag="VNW28_01055"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXA08536.1"
                     /translation="MLRRRRFLTCIFLASAVADLFAGTGTTIPPRVIISVRDQKLMVV
                     ENGERVAVYPVSTSKFGLGDRWGSMATPLGWLQVAEKIGDHAPTGAVFHKRRFTGEIL
                     PPNAPGRDPIVTRIIWLRGLEPSNANAFNRCIYIHGTPEEKIIGKPASYGCVRMSSKD
                     VADVYAQVQIGALVRIIPDSLPKLPKSKTPPGKAIFTAAAPPPTSPPVATSGAIRKAD
                     SKRKRVSTAAAPHSDGSA"
     gene            complement(5170..6672)
                     /locus_tag="VNW28_01060"
     CDS             complement(5170..6672)
                     /locus_tag="VNW28_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase family protein"
                     /protein_id="HXA08537.1"
                     /translation="MGGSVVDVIWAFCYLSVLIGLSGYGLHRYFIVYLFLKNRKRAPR
                     PLAHFQQLPVITVQLPLFNEIYVVERLLRSVSELDYPRERLQIQVLDDSTDETRELTA
                     ACVRRLAARGFDVELVHRTNRVGYKAGALEAGLATARGEFVCILDADFVPQPELLKAT
                     VHFFTDPKVGMIQTRWGHINRGYSLLTRVQAMFLDGHLLLEQVARSRSGRFFNFNGTA
                     GLWRKSCIVDSGGWQHDTLTEDLDLSYRAQLEGWKFVFLTDVVTPAELPVDMNGFKSQ
                     QHRWTKGSIQTCKKLLPRIWRSGLPLPIKIEATGHLTSNFAYLLLACLCVLLHPSTGG
                     PQSGWLRMLLIDLPIFLTASVSVAVFYICAQRELHPRTWMKEMLLLPALIALGVGLSI
                     NNARAVLEAVFNHPSEFKRTPKYGIETKSQSWRSCRYMPLKSLLPVVEIGFAIYFSYF
                     VWYAVTHEQYFTLPFLVMFQGGFLYVALSSLAQWWPRFNFPSAREIAFPA"
     gene            6986..9460
                     /locus_tag="VNW28_01065"
     CDS             6986..9460
                     /locus_tag="VNW28_01065"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXA08538.1"
                     /translation="MQEQAKLRSRPRGRFERLNIVIYILFAGWVPSILMLVVSYTILT
                     RTLESKIQRDRQTFVQLIAHLVGDDIGRSGAIVDYYQTFPPVAKIFAARYPDLAAKSW
                     LQETYFSHPRIDGMFITTADGRLIASLPDDPAMTLTNQISQSWLAAAGTAKSFYVSPV
                     HPRTSDNRAATDLVSPIKAPDGSRIGFIGVSVLVERIGRRLSTIDLADQARCQIVDQN
                     GHELFSNDFKANSGPASPATRTLINDIRALKTGHIEREGNLYSFAPVESTGWTTVVEQ
                     PRAVAYQPVRDLLERMTVLATWLIVGTGIIAWLTGKSYRRQREAAARIEREVIFNDKI
                     LANMPSGIALVDPTTRKFLQANEAFGQMARRFGNLPPEKEMNEATYQEVNIAPPEAIE
                     KVLSFGTPFQLVEQPVVDRAGITNFVNVNLLRLQDSKQTVQGVLFLVEDKTRDVRLRQ
                     ELISANAAKDQFLALLSHELRNPLSPVIAMVGELEARTPDEPELRQPLAVIRRNVELE
                     ARLIDDLLDVTRIAKGKLQLSFEVTSIHETLERAYEICREDILQKKLRFEFRLGAEHE
                     YVNGDPARLQQVFWNLIKNGVKFTPAGGQITVETSNLDDDHIAIRTIDNGIGIDHGKI
                     EKIFNAFEQGQSSITRKFGGLGLGLAISKAMVRAHGGTISVESAGLGRGSIFSVVLNT
                     VPAPAEAVVPAGSPNGGEEKPPARKGHLLVVDDHHDTCVGMKMLLERRGYRITLAHTA
                     DQAIEKAQLEKFDLLISDIGLPDRSGYELMRELQAKNSLRGIALSGFGMEHDINKAHA
                     AGFSEHLTKPINFDRLDEVIRSLLVE"
     gene            complement(9478..10056)
                     /locus_tag="VNW28_01070"
     CDS             complement(9478..10056)
                     /locus_tag="VNW28_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXA08539.1"
                     /translation="MADISNHLREAIRAACSTFESLQDLAQPIARAADAMVDCLCGGR
                     KLLVCGNGGSAADGADFSAEFGCRFVTDRRPYPALNLAQGGSLMSAIGNDYGYEEVFA
                     RQVVGLGQRGDILVVITTSGNSRNILRALEEGKTRGLTTVALLGRHGGLARGLAEIEL
                     LVRSESTARIQEAHKFLLHVICGLVDARLPKA"
BASE COUNT         2151 a         3176 c         3187 g         2036 t
ORIGIN      
        1 tgccgatgcc catttcaatc tggcggtcgt ctattccacc tcccaaccac cggcgaagga
       61 attggcgcgg cgccattacg aaaaggcgct cgctctcggc gcggcgcccg acccgacgct
      121 cgacaaactc ctgcgctgag cacggccttc ctcaccgagg aatggcggca tctcgtgatg
      181 ctcaattacg cggtcgagca taaattgctc gacccacgcg ttccgcgcgg cctggaactc
      241 gatcgcttcc actcggtcgc ttacctcagt gttgttggtt ttcttttcct gaaggcgcgc
      301 gtcctcggaa tctccttccc gtttcaccgc gactttgagg aagtaaatct gcgcttttac
      361 gttcgccatt gggggccgga gggctggcgg cgcggcgtcg tcttcgtgcg ggagattgtc
      421 ccccgtcggg cgatcgccac cctcgcgcgg cgcttgtatg gcgaaccgta ttccgccttc
      481 ccgatgatgc acgcgatcga gcgcgacggg ctcgagttgc gttgccgtta tgcatggcag
      541 cgaaatgggc ggcgtgaatt tgtttccgcc tccgcgtccg gcgcggcgga accaatcgcc
      601 tccggatcgc tggaggaatt catcaccgaa cattactggg gctacacggc acgcggcgaa
      661 cactgcacgc agtatcaggt cgaacatccg cgctggcgag tttggcgcgc gaccgcggcg
      721 cgattcgagg ccgatgtcgc cagcctctac ggcccggaat ttgtcgcgcc gctttcggcg
      781 ccgcccgcct cggccttcat cgccgacggt tcgcccgtcg tggtgcgacg cggcagtcca
      841 ctcgcctcag ccgccgtggc caccgagtaa ctcgcgcagg acaaatggaa ggatgccacc
      901 gtggcgataa taatcgatct cgatcggggt atcgatccgt agcttcaccg gcacggcctg
      961 ctcttgacca tcggcccgcg tgatttcgag ggtgagattc tgcccgggct gaatcgactc
     1021 cgaaaggccg gttacactgt agacctccga gccgttcagg cctaacgact gcgcattggt
     1081 tccttccgca aactgcaatg ggagcacgcc catcccgacc aggttggagc ggtgaatgcg
     1141 ttcgtagctc gccgccacga cggctttcac gcctaacaat cgcgtgcctt tcgccgccca
     1201 gtcgcgactc gagccggtgc catactcctg tccagcgaga atgatgagcg gggtttttgc
     1261 ttccgcgtat ttcagcgccg cgtcgaagat cgacatttgc tcgccggtcg ccgcctgccc
     1321 gtggccgaaa aacttcgtca cgccgccttc cacgcccggc accatcaggt tttttatccg
     1381 cacgttcgca aacgtgcccc gggtcatgac gaggtcgttg ccgcggcgac tgccgtagct
     1441 gttaaaatcc tccggtttga tgccgcgtga gaccaggtat tgcccggccg gcgaactcgc
     1501 cttgatcgcg cccgccggcg agatgtgatc ggtcgtgacc gaatcgccga agatgcccag
     1561 cggccgcgcg ccgcggatgt cctcgatccg tccgggcgcc atcgagaaat cggcgaaaaa
     1621 tggcgggtcg tgaatgtagt cgctctcttc gtcccactgg taaacctcgc cggtgctgga
     1681 ggaaatttcg ttccacttcg gattctggcc ggcgaagtcg cggtagagtt tgcggaatat
     1741 ttcgggagtg agcgcggact gcatcacggc gcggatttcg cctaaagacg gccagagatc
     1801 cttcagaaag accgcgcgac cctctttgtc ctttccgagc ggatccttgg cgaggtcgag
     1861 atcaacccgg cccgcgagcg cgaaggccac gaccaggggc ggcgacatga gaaaattggc
     1921 cttgatgttc tggtggacgc gcgcttcgaa attgcgattg ccggaaagga cggaggcggc
     1981 caccagatcg aattcgttga tcgcctgctc gatcttcgga tggagcgggc cggagttgcc
     2041 gatgcaggtg gtgcagccat agccgacgat attgaagccg agctggtcga gatattcttg
     2101 cagcccggtc ttgtgcaggt agtcggagac gacgcgcgac ccgggcgcga gcgaagtctt
     2161 gaccgaggga tcgatcgtta ggccgcgttc caccgctttt ttggcgagca ggccggcggc
     2221 gagcatcacg ctcgggttgc tcgtgttcgt gcagctggtg atcgcggcga tgagcacgct
     2281 gccatggccg atgcagcttt gcccctgggc aaaaacttcg ccatcctcgg cctcgatcgc
     2341 gctccccttg tccggtgtcg gacggttcga aaccatttca atcttgttgc gttcgtcgcc
     2401 cggctcgatg ccttgatccg gccgatcggt ggaaggcatt tcctcggtct tgggaggccc
     2461 gccgttgaga ctgacgagat gccgccggcc aagctcggcg ccggcttttc cgtagccgcc
     2521 gtccttcacc ggtttctcga gcaacgaacg aaaagcgctg ccgagttccg gcaggttaat
     2581 ccgatcctgc ggccgcttcg ggccggcaac gctgggctgc acatcaccca ggtcgagctc
     2641 gagatcgacg ctgtaatcga tctcgccttg gcgcggcatg ccgaacattt tctgcgcgcg
     2701 gaaatacgct tcgaaagcgc ggcattgctc gtctgtccgg cccgtcgcgc gcaggtaatt
     2761 caccgactcg gcatcgaccg ggaaataacc catcgtcgcg ccgtattccg gtgacatgtt
     2821 gccgaccgtc gcccgatcgg gcaacggcaa cgaggcggcg ccctcgccgt aaaattcgac
     2881 aaatttcccc accacctttt gcgcgcggag catctgggtg atgtggagcg cgagatccgt
     2941 cgcggtgacg ccctcgcgaa gcgaaccacc gatgtgcacc ccgacaactt ccggcgtcag
     3001 aaaataaacc ggctgcccca gcatgccggc ttcggcttcg attcccccga cgccccaacc
     3061 gacgacgccg aggccattga tcatggtcgt gtgtgagtca gtcccgacca gcgtgtcggg
     3121 atagtaaacg tcgccgctct gcaacacccc cttcgcgaga tattcgagat tgacctggtg
     3181 cacgatgccg atgcccggcg gcacgagacc gaaagtcttg aaagcttgct ggccccactt
     3241 gagaaattcg taacgctcgc ggttgcgctt gaattccatc tccatgttca accgcaacgc
     3301 ggcggccgag ccggcaaaat caacctgcac agagtgatcg accacgagat cgaccgggac
     3361 gagcggctcg atgattttgg gatcgccgcc gaggcgtcgc accgcgctac gcatcgcggc
     3421 gagatcgacc accagcggca cgccggtgaa gtcctggaga acgatccgcg cgaccacgaa
     3481 cggaatttcc tcgttcgccg gcgccttcgg gttccagttg gccagcgtct cgacatcttt
     3541 gcggccgacc ttctggccgt cgcaattccg gagcaccgat tcgagaacaa ttcgaatgct
     3601 gaccggaagg cgggagatgg gaccgacgcc ccgttcctcg agcgcgggga gcgaatggaa
     3661 aaacgccgcg cgcccgtgac cggcgtcgaa cttttgcaag gtatcgaact cgttaggcat
     3721 gattatttcc tattccgtcg cgggagcttt tgggttgcat cacgggatcc ctcaacttca
     3781 cctgggatga cggtggcggc cgtcacttta gctcggccag tctggccttg atcttgtcga
     3841 agtccggcaa ctcgcgcgca tcctcgtgca cctcggcata ttggatgacg ccagcgcggt
     3901 cgatgaggaa ggcggagcgc ttcgcgacgc ccgcaaagcc gagatgcaat tgggggagga
     3961 acgattcgta cgcgaccccg tagctgtgcg ccaccttgtg ttcgtagtcg ctcaggagcg
     4021 tgacgttgat cccttccttt tgcgcccagg cttcctgggc aaaggggttg tcgccgctga
     4081 tgccatagac ctcgcaatgg agatcgccat actcgcgcaa gccgttgccg acaccgcaca
     4141 tctcagtcgt gcaggtgccg gtaaaggcca tcggaaagaa gagcaaaagc gtgttcttga
     4201 caccgaagtt ttcgctcaac cgaaccgggc gcggcccgtc cgcgttcttg gtggggagag
     4261 tgaaatctgg tgctttggtt ccaactgcga gtgccataat gaatgctccg gtgaggttgc
     4321 gcgggagaca accagtatag aaacaggcaa ggcggggtca aaagatttgc gggccccccg
     4381 ccgaatggaa gccggactcc gatcaggctg gggctgtccc cgtcgttagg cggagccgtc
     4441 ggagtgaggc gcggcggcgg tggaaacgcg cttgcgtttg gaatccgctt tgcgaatcgc
     4501 gcccgacgtg gccaccggag gactcgtcgg cgggggagca gcggcggtga aaattgcttt
     4561 gccgggggga gtcttcgact tcggcagctt cgggagcgaa tcgggaatga tcctgacgag
     4621 cgcgccgatt tggacctgag cgtaaacgtc ggcgacgtcc ttggaggaca ttcggacgca
     4681 gccgtaactg gcgggtttgc cgatgatctt ctcctcgggt gtgccgtgga tgtagatgca
     4741 acggttaaag gcgttggcgt tcgacggttc gaggccgcgc agccagatga tgcgggtgac
     4801 gatcggatca cggccgggag cgttgggcgg gagaatttca cccgtgaaac ggcgtttgtg
     4861 gaaaaccgct cccgtgggag cgtggtcgcc gattttttcc gccacttgca accagcctaa
     4921 cggcgtcgcc atgctccccc aacggtcgcc gaggccgaac tttgaagtgg agaccggata
     4981 aaccgcgact cgctcgccat tttcgaccac catcaatttt tgatcgcgca cgctgataat
     5041 gacgcgcggc ggaatggtgg tgccggtgcc ggcaaacaaa tcagcgactg ccgaggcgag
     5101 aaaaatacag gtgagaaagc ggcggcggcg aagcatcggc gtcctaatta acgagcagag
     5161 ggcaccatgt cacgccggaa atgcaatttc ccgcgcactc gggaaattaa agcgaggcca
     5221 ccactgggca agcgaggaga gagcaacgta gagaaatccg ccctgaaaca taacgagaaa
     5281 aggaagggta aaatactgtt cgtgggtcac cgcataccag acaaagtagc taaagtaaat
     5341 cgcaaatccg atttcgacga ccgggagaag cgatttgagc ggcatgtaac ggcacgatcg
     5401 ccagctctgt gatttggttt cgatcccgta tttcggggtg cgcttgaatt ccgaagggtg
     5461 attgaagacg gcttcgagca cggcgcgggc gttgttgatc gaaaggccga ctccgagagc
     5521 gatcagcgcc gggagcagaa gcatttcctt catccaggtg cgcgggtgga gttcgcgctg
     5581 cgcgcagatg tagaagaccg ccaccgagac cgacgcggtg aggaaaatcg ggaggtcgat
     5641 cagcaacatg cgcagccagc ccgactgcgg tccgccggtg gagggatgca ggagcacaca
     5701 aaggcaggca aggaggaggt aggcaaaatt tgaggtgaga tggccggtcg cctcgatctt
     5761 gatcggcagg ggcaggccgc tgcgccagat gcggggcagt aatttcttgc aggtctggat
     5821 cgagcctttc gtccagcgat gctgttgtga tttgaagccg ttcatgtcga cgggcaattc
     5881 tgccggcgtc acgacatcgg tcaggaaaac gaacttccag ccttcgagct gcgctcgata
     5941 gctgagatcg agatcctcgg tcagcgtgtc atgttgccag ccgcccgaat ccacaatgca
     6001 ggatttgcgc cagagaccgg cggtcccgtt gaaattaaag aaacgcccgc tccggctgcg
     6061 cgcgacctgc tcgagaagca gatggccatc gagaaacatc gcctgcacgc gggtgaggag
     6121 cgaatagccg cggttgatgt gcccccaacg cgtttgaatc attcccactt tcggatcggt
     6181 gaaaaaatgg acagtggctt tgagcaactc gggttgcgga acaaaatcgg cgtcgaggat
     6241 gcagacaaac tcgccgcgcg cggtggcgag cccagcttcg agcgccccgg ctttgtagcc
     6301 gacgcggttg gtgcggtgga ccaactcgac gtcgaagccg cgcgcggcca gtcgccggac
     6361 gcatgcggcc gtgagttccc gggtctcatc ggtggaatca tcgagcacct ggatctgtaa
     6421 gcgctcacgg ggataatcga gttcactgac cgacctgagc agacgctcga cgacgtagat
     6481 ttcgttgaaa agcggcaact ggacagtgat gaccgggagc tgctgaaaat gggcgagcgg
     6541 ccgcggagcc cgcttccggt ttttcagaaa aagatagacg atgaaatacc gatgcaggcc
     6601 gtagcccgag aggccgatca ggacactcag gtaacagaac gcccagatta catccacaac
     6661 gcttcctccc ataacaatgc taacagtgcc gcactggtca aggcgcacag aggcgacatg
     6721 atgccgcttc cgcgggatgc gtcaaatcaa atctcgggtg gcgcggcggt gcaatcaggc
     6781 gcctggcgaa gcgtccgccc aaggcaattc gcctgtagag gcgagcgctt tgcccgttcc
     6841 gtgcgaattg agttcacagg caaacgccca aaaatcgcga gccccggcga gctacctgcc
     6901 tccggttcga cgtcaaaatg acatcacaac cggttgcgtc gcggaggaag aagccgaaaa
     6961 ctgaccaggc accccgaccg aaatcgtgca agagcaagct aaactgcgca gccggcctcg
     7021 cggccgtttc gagcgtctca acatcgtcat ctatatcctg ttcgccggct gggtgccgtc
     7081 gatcctgatg ctggtggtct cctatacgat cctcacccgg acgctcgaat caaaaatcca
     7141 gcgcgaccgc cagactttcg tccaactgat agcgcacctc gtgggggacg acattggccg
     7201 gagcggcgcg atcgtcgatt attatcaaac attccctccc gtcgcgaaga tttttgcggc
     7261 ccgttatcca gacctggcgg ccaagagctg gctgcaggaa acctatttct ctcaccctcg
     7321 catcgacggg atgttcatca ccacggcaga tggccgcctc atcgcctcgt tgcccgatga
     7381 tccggcgatg actctgacca accagatttc gcaatcctgg ctggccgccg ccggcacggc
     7441 caaaagcttt tacgtctcgc cggtccaccc gcggacgtcg gataaccgcg ccgcgaccga
     7501 tctcgttagt ccgatcaagg cgcccgacgg ttcgaggatc ggcttcatcg gcgtctcggt
     7561 cctggtcgag cggatcggac ggcgcttgtc gacgatcgat ctcgccgacc aggcgcgctg
     7621 tcagattgtc gatcagaatg gtcacgagct tttcagcaat gatttcaaag cgaattccgg
     7681 cccggcgtct cccgcgacca ggactttgat caacgatatt cgcgccctca agaccggaca
     7741 catcgagcga gagggcaatc tctattcatt cgcgccggtc gagtcgacgg gttggaccac
     7801 ggtggtcgaa cagccacgcg cggtcgccta ccaaccggtg cgcgatctac tcgagagaat
     7861 gaccgtgctg gcgacctggc tgatcgttgg gacgggcatt attgcctggt tgactggaaa
     7921 atcgtaccga cgccaaaggg aagcggcggc gcggatcgaa cgggaagtca tcttcaacga
     7981 caagatcctg gccaatatgc caagcggaat cgcgctcgtc gatccgacga cgcggaagtt
     8041 tctccaggcg aacgaagcct tcgggcaaat ggcgcgacgg ttcggaaatt tgcccccgga
     8101 aaaggaaatg aacgaagcga cctaccagga ggtaaacatc gcgccgcccg aggcgatcga
     8161 gaaagttctc tcgtttggga cgcccttcca actcgtcgag cagccggtcg tcgatcgcgc
     8221 cggcataacg aacttcgtca atgtaaatct gctccgcctg caggattcca aacagacagt
     8281 gcaaggtgtg ctcttcctgg tcgaagacaa gacgcgcgat gtgcggctgc ggcaggagtt
     8341 gatctcggcg aacgcggcca aggaccagtt ccttgccttg ctctcgcacg agctgcgcaa
     8401 cccgctctcg cccgtgatcg caatggtggg cgagttggag gcgcgcacgc ccgacgagcc
     8461 cgagctgcgt caaccgctcg ccgtcatccg tcgcaacgtg gaactggaag cgcgcttgat
     8521 cgacgacctg ctcgacgtga cccggatcgc gaaaggcaag ctccaactta gtttcgaggt
     8581 cacttccatt cacgaaacgc tggaacgcgc ctacgaaatc tgtcgcgagg acattctgca
     8641 aaagaaatta cgctttgagt tccggctcgg cgccgaacac gaatacgtca atggcgatcc
     8701 ggcccgattg cagcaggttt tttggaacct gatcaagaat ggcgtgaaat tcactcccgc
     8761 cggcgggcaa attaccgtcg aaacctccaa tctcgacgac gaccacatcg cgatccgtac
     8821 aatcgacaat gggattggca tcgaccacgg gaagatcgag aagattttca atgccttcga
     8881 acagggccaa agctccatca cgcggaaatt cggaggactc ggactcggac tcgcgatttc
     8941 caaggcaatg gtgcgagccc atggcgggac gatctccgtc gagagcgccg gcctgggcag
     9001 gggatcgatc ttcagcgtcg tgctcaacac cgttccggct ccggcagaag ccgtcgtgcc
     9061 agccggctcg cctaacggcg gcgaggaaaa acctccggcc cgcaaaggcc acctcctcgt
     9121 ggtcgatgat catcacgaca cctgcgtggg gatgaagatg ctgctggaac gccgcggcta
     9181 ccgaattacc ctcgcccaca cggctgatca ggcgatcgaa aaagcgcagc tcgagaagtt
     9241 cgatttgctc atcagcgaca tcgggttgcc cgaccgcagc ggctacgaac tgatgcgaga
     9301 actgcaggca aaaaattcgc tgcgcggcat cgccttgagc ggttttggaa tggaacatga
     9361 catcaacaaa gcgcacgccg ccggtttctc agagcaccta acgaaaccga ttaatttcga
     9421 ccggctggac gaagtgattc gcagcttgtt ggtcgagtag cggaggcgcc ttgagcgtta
     9481 cgccttcggc aggcgcgcgt ccacgagccc acaaatcacg tggagcagaa atttgtgcgc
     9541 ttcctggatc cgcgccgtcg actcgctgcg cacaagaagc tcgatttctg ccaggcccct
     9601 ggccagcccg ccgtgccggc cgaggagcgc gaccgtcgtt aggccgcgcg tttttccctc
     9661 ttccagcgcg cgcagaatat ttctggaatt accgctggtc gttatcacaa ccaggatatc
     9721 gccccgttgc ccaagtccga ccacctgccg cgcgaagact tcttcgtagc cataatcgtt
     9781 gccaatggcg ctcatcaggc tgccaccctg cgcgagatta agcgccggat agggtcgccg
     9841 gtcggtcaca aaacggcagc caaactccgc cgagaaatcc gcgccatccg ccgcgctccc
     9901 gccattgccg cagaccagta acttccttcc gccgcagaga caatcgacca tcgcatccgc
     9961 cgcccgtgcg atcggttgcg ccaggtcttg caatgactcg aaagtgctgc acgcggcgcg
    10021 aatcgcctcc cgtagatgat tgctgatgtc tgccatgtat ctttgtcctc tccttcgcaa
    10081 ttcgagcagt ttggctgccg atacaacctc ggcgcatgtg ttgcaaccga tatcgtggca
    10141 tcccgtcgag cgcagaggca agacggtgaa aacctgaccc aaatgtcagt ttcgcttgcg
    10201 cttttgttga ttatctgtcc aaatgaaccg gacaatccag tcgaacgccg cgaggcggtc
    10261 gttcggccgc cgaacgtcgt tccgctcagg ggcaagcgga gtagatacgc atttgaatta
    10321 cagagtttgc tttaagactc agccagcggc gggtttcgtg ctcgatgcgc caaatccggg
    10381 tatcggaaga attttgtata cagaatttcc aaaacttcat aagctttagc agaaatcagc
    10441 aacctgcctt cttaaattat gagagacttt tcaattgccg atgaaacccg acagcagcgc
    10501 aacggaactg ggacggtcgt tcccgtgacg ctttcggtgg tcttcccggc
//
LOCUS       DATMNS010000026         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3597757, whole genome shotgun sequence.
ACCESSION   DATMNS010000026 DATMNS010000000
VERSION     DATMNS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3597757"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..406
                     /locus_tag="VNW28_01075"
     CDS             29..406
                     /locus_tag="VNW28_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08540.1"
                     /translation="MKTALQAGMQATLPLGQATTISSKATRPPPAPAAARNYAAVEIH
                     SYLAARDLLFREAEENPTDDILRRAMAANETVENCLRPARAPYQAQSLPEAEAARERQ
                     HCKAVKLRIAELRARAGMRRHAA"
     gene            complement(471..1997)
                     /locus_tag="VNW28_01080"
     CDS             complement(471..1997)
                     /locus_tag="VNW28_01080"
                     /inference="COORDINATES: protein motif:HMM:NF005545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXA08541.1"
                     /translation="MKTSVAVIGAGPAGLTAGYLLAKNEVPVTILEADPVYVGGISRT
                     ANYKGFHFDIGGHRFFSKSKAVDDLWNEILPDDMLERPRSSRIFYDKKFFAYPLEPFE
                     ALRKLGLFRSAACVLSWLKARLFPIANPRNFEEWVSNQFGRRLFKTFFRSYTEKVWGM
                     DCREISADWAAQRIKGLSLGSAIRNALFPPRQPRDKSKVIKTLLQSFRYPRKGPGMMW
                     EAAARKMQAWGGELLPGCTVTGCAYDERTEKWTVHFRARGGGEQTLEADHVISSAPMR
                     ELIRGLTPAVPERVRAAAESLKYRDFLTVMLVVRAEDAFADNWIYIHDPSVKVGRIQN
                     FRSWSPEMVPEPDKACYGLEYFCFEHDGLWDSGDEELIELAKRELGQIGLARPGDVVD
                     GCVVRQKKAYPVYDDDYARHVATIRTELDQHYPNLHLVGRNGMHKYNNQDHAMMTAML
                     CVENILAEEKLYDLWQVNGDAEYHEAGAAETAPEYGTALRLVPMRAAATPELASEQTG
                     "
     gene            complement(2005..>2902)
                     /locus_tag="VNW28_01085"
     CDS             complement(2005..>2902)
                     /locus_tag="VNW28_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08542.1"
                     /translation="FWTYFFLFCQRWELPKSLQVFGALALAAHPAAFYLVAGYSESLF
                     LMTLVGFIYWSTSERRGAKVFAVLHGFTMSATRIVGLVCATFPVVQALFHHDSRPNEK
                     LRDRIARYRAPLLIVLFSMLGGVTFFLYCQVRWGRWDLYLLTQESGWAIDADYGAIFK
                     LENYHFALPSLDDTTAASQFTSAVAGVLFVVIALVEVIAAFRRRSGWRQRVGLYFSAA
                     AIYYISFAGVASVQLESMLRYDLCVHALVILGLLHFLREFRMPPGYVRALAMGVAALV
                     CAAGLGLQGYYVWNFTQGNWVA"
BASE COUNT          598 a          920 c          875 g          509 t
ORIGIN      
        1 gtcagccgga attccctctg gcccaagcat gaaaactgct ttgcaagcag gcatgcaagc
       61 gactctcccg ctcggtcaag ccaccacgat ttcatccaaa gccacccgtc caccgcccgc
      121 accggccgcc gcccgaaatt acgccgccgt tgagatccac tcctatctgg ccgcgcgtga
      181 tttgcttttt cgggaagcgg aagagaatcc gaccgatgac attttacgcc gcgccatggc
      241 ggccaacgag acggtggaga attgcctccg gcccgcccga gcgccctacc aggcgcagtc
      301 gcttcccgaa gccgaagccg cgcgcgaacg ccagcactgc aaggcggtca agctgcggat
      361 tgccgagctc cgggcccgcg ccgggatgcg tcgtcacgcc gcttaatcac gtcgttaggc
      421 gacgagccgg gtgcgcctgg caaccggcgt ttcgttaggc aaccgctcct tcatcccgtc
      481 tgttccgaag ccagttcagg agtcgcggcc gcgcgcatcg gaaccagccg aagggcggtc
      541 ccgtactcgg gcgcggtctc cgcggcgccc gcttcgtgat actcggcgtc gccgttcacc
      601 tgccagagat cgtagagctt ttcctcggcc aggatgttct cgacgcaaag catcgcggtc
      661 atcatcgcgt gatcctggtt gttgtatttg tgcatcccgt tgcgtccgac aagatgcagg
      721 ttcgggtaat gctggtcgag ctcggtccgg atcgtggcca catgccgcgc ataatcgtca
      781 tcgtagaccg gataggcttt tttttgccta acgacgcagc catcgaccac gtcgcccggc
      841 cgggccagcc cgatctgccc gagctcgcgt ttcgccagtt cgatcaactc ttcatcgcct
      901 gaatcccaca gcccgtcgtg ctcgaagcag aaatactcga gcccatagca ggccttatcc
      961 ggctccggca ccatctccgg cgaccacgag cggaagtttt ggatgcgacc gacctttacg
     1021 ctcgggtcgt ggatgtaaat ccagttgtcg gcgaaggcgt cctccgcgcg cacgaccagc
     1081 atgacggtga gaaagtcgcg atatttcaaa ctctcggccg ctgcccggac ccgctccggg
     1141 accgcgggcg tgagcccgcg aatcaactcc cgcatcgggg ccgaggaaat gacgtggtcc
     1201 gcctcaagcg tctgctcgcc tccgccgcgc gcgcgaaaat gcaccgtcca cttttcggtc
     1261 cgttcgtcgt aggcgcagcc ggtcaccgtg caacccggga ggagttcgcc accccacgcc
     1321 tgcattttcc gggcggccgc ttcccacatc atgccggggc ctttccgcgg gtaacggaag
     1381 gattggagaa gcgtcttgat gactttcgat ttgtcgcgcg gctggcgcgg cgggaaaagg
     1441 gcgttgcgga tcgcgctgcc taacgataag cccttgatcc gttgcgccgc ccagtcggcc
     1501 gagatttcgc ggcagtccat cccccagact ttctcggtgt agctccggaa gaaggtcttg
     1561 aagagacggc ggccgaactg gttgctcacc cattcttcaa aattgcgcgg gttcgcaatc
     1621 gggaagaggc gcgccttaag ccacgaaagg acgcaggcgg cggagcggaa aaggccgagc
     1681 ttgcgcaggg cctcgaatgg ctccagcggg taggcgaaaa acttcttgtc gtagaaaatg
     1741 cgcgaggagc gcggccgctc cagcatgtcg tcaggcaaaa tctcgttcca gagatcgtcg
     1801 accgccttcg atttcgagaa gaaacgatga ccgccaatat cgaagtgaaa acccttgtag
     1861 ttggcggtgc gcgaaatgcc gccgacgtaa accgggtccg cctccaggat ggtgaccggg
     1921 acctcgtttt tcgcgaggag ataacccgcg gtcagtcccg ccggacccgc gccgatcacg
     1981 gcgacggagg ttttcatttc gctttcaagc gacccagttt ccctgagtga agttccagac
     2041 ataatatcct tgcaaaccga gtccggcggc acagacgagt gcggccacgc ccatggccag
     2101 ggcccgcaca taccctggcg gcatccggaa ttcccgcagg aagtggagca atcctaggat
     2161 aacaagcgcg tgcacgcaaa gatcgtagcg cagcatgctt tccagctgca ccgaagcgac
     2221 cccggcgaat gaaatgtagt agattgccgc ggccgaaaaa tagagaccga cgcgctggcg
     2281 ccagccgctc cggcggcgga atgcggcgat cacttcgacc agcgcgatca cgacaaaaag
     2341 cacaccggcc acggcgctgg tgaactggct ggccgccgtc gtgtcatcca gggacgggag
     2401 cgcgaagtgg tagttctcca gtttgaagat cgccccataa tcggcgtcga tcgcccaacc
     2461 ggattcctgg gtgagcagat agagatccca gcgtccccag cgcacctggc agtaaaggaa
     2521 aaacgttact ccgccgagca tcgagaacag cacgatgagc agcggcgcgc gataacgcgc
     2581 gatccggtcg cgcagttttt cgttaggccg ggagtcgtgg tggaaaagag cctgcaccac
     2641 cggaaacgtg gcgcaaacca ggccgacgat gcgcgtggct gacattgtga aaccgtgcag
     2701 gaccgcaaac accttcgctc cgcgccgctc gctcgtgctc caataaatga acccgacgag
     2761 ggtcatgaga aagagcgact ccgaatagcc ggcgacgagg taaaaggctg ccggatgcgc
     2821 cgcgagcgcg agcgcgccga aaacctggag cgatttcggg agttcccatc gttggcagaa
     2881 gagaaaaaag taagtccaaa at
//
LOCUS       DATMNS010000027         3762 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_37160, whole genome shotgun sequence.
ACCESSION   DATMNS010000027 DATMNS010000000
VERSION     DATMNS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3762
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37160"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..550
                     /locus_tag="VNW28_01090"
     CDS             107..550
                     /locus_tag="VNW28_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08543.1"
                     /translation="MQKYLNLAVSAALLLTSAGLCWAQSDAPYNEGPVWRISMIKTKP
                     GMTDDYLKGLAKTLKGEMEEFKKQSIILDYKIMIGETATPQDYDILIMVESKDMASLD
                     NLRERTDPIANKIVGSADKQRATAVKRLEIREIMGTKLMREITLK"
     gene            complement(737..1738)
                     /locus_tag="VNW28_01095"
     CDS             complement(737..1738)
                     /locus_tag="VNW28_01095"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HXA08544.1"
                     /translation="MIRPRFIVRWQFVFCGLLLVASLAGTLTRAELTEEEKKELFLKS
                     REKMRTVGSPTPEESPTPRHKPQPAKHPPAKKKKSATPRPTPEPEETPESTPQPTAQE
                     TPARHPTPEESTPAHAVAVTPPPRPPASAAPGASAAPAQVVIEKSGYQKPSGFSLLPF
                     HLFGKHYTYLTSSVRREIDRAPVLRERWRFIIVHNSGTRQGNARIFDYYHRNVRRMTN
                     GLAYHFVIGNGTSSGNGEIEIGDRWRRQINGGHVHSDYLNNISLGICLVGDFNRDQPT
                     RAQLDSCEELIRYLRARCGKVDRHDIPVRPHREMNPPRWPTDCPGDAFPYRWFGQFR"
     gene            complement(1752..2645)
                     /locus_tag="VNW28_01100"
     CDS             complement(1752..2645)
                     /locus_tag="VNW28_01100"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXA08545.1"
                     /translation="MKILRLTGRVVRHLVWETATDETMGLSGQLAYQFLFTLAPGLLF
                     LWHLLGLFGTDPAKLHKMLGVVRAFLPPDPKVQEILDSVVANVIVTGSGGALATAGIF
                     LGIYLGTMFIATISRALSRTHGVTEDRHWWSKYIISLLLLFWFGITIIFAFNLTVFGE
                     TLAGIAEVNFQLRIPLQEWVAALNIPFTALALVVLALALYLLTPQNYLTIRQALPGAI
                     FFSVGWMTVTKLFQFYVARYDRYNPTYLALASLIMLLTWMYLTCLLLLLGGKLNAILR
                     RESEDAAARAEQAAASEPQPA"
     gene            2716..>3762
                     /locus_tag="VNW28_01105"
     CDS             2716..>3762
                     /locus_tag="VNW28_01105"
                     /inference="COORDINATES: protein motif:HMM:NF018613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA08546.1"
                     /translation="MEKRILILTAGYGEGHNAAARGIRDGLATVAGDAAMAEVHDLFA
                     ETYGVTNDWVRKGYLALINRAPRVWKRFYDWTDRQEDFSASMRWIFQAKNRLSQLLVR
                     FQPTAVVSVFPAYPHLLEEILGDSRPAFRRVVCITDSITVNKIWFRCASDLFLAPNEQ
                     TAAVLAASGVPADKVRVSGFPVTPKFAELGELRQPPAPDSRRRLLYVINSGGLAPPEI
                     IRGLALPDVRLTVTVGRDERLRKTVEAVRAEAGRDFEIVGWTDQLPRLMLESHLLISK
                     AGGATVQETIAAKCPMIINQIVPGQEEGNARLIEETRSGVIATTPATISAAVQRAFAD
                     DARLWREWSENIGRI"
BASE COUNT          733 a         1044 c         1311 g          674 t
ORIGIN      
        1 gccttgatgg gcgctaaaat tgtgttgacg gccgttggtc tcaaaccaca ctcgccgcga
       61 ttggctcacg acgagccgct catcaaccat agaaagaaat cccaaaatgc aaaaatatct
      121 taacctggcg gttagcgctg ccttgctcct aacctcagcc ggcctgtgct gggcgcaaag
      181 cgacgccccg tacaacgaag ggcccgtctg gcgcatctcc atgatcaaga ccaaacctgg
      241 catgaccgac gactacctca aaggtctcgc caagacgctc aagggcgaaa tggaggaatt
      301 caaaaagcaa agcatcatcc tcgactacaa gatcatgatc ggcgagaccg ccaccccgca
      361 ggactacgac attctgatca tggtggaatc gaaagacatg gcatcgctcg ataacttgcg
      421 ggagcggacc gacccgatcg cgaacaagat cgtgggcagt gccgataagc aacgcgcgac
      481 cgcggtcaag cgtttggaga ttcgcgagat catgggcacc aagctcatgc gcgagattac
      541 gctgaaatag ttatccgcgc accgggacga cacgccgcga tttaggtcgc ggcgcgtctc
      601 cttcctgccg gggtaggaac aggttgggtt aaccgcagag agcgcagagg tcgctgagtg
      661 gggatgaatt aatccgggtc aatctctgcg cactccgcgt cctccgcggt taaatccgca
      721 tttcggtcgc ggcccactaa cggaattggc cgaaccagcg ataggggaaa gcatcgccgg
      781 gacaatcggt cggccagcgg ggcggattca tctcgcgatg ggggcgcacc gggatgtcgt
      841 ggcgatcgac cttgccgcag cgcgcgcgca ggtagcggat cagttcctcg caggagtcga
      901 gctgggcgcg agtcggctgg tcgcggttga aatcgccgac gaggcagatg cctaacgaaa
      961 tattgttgag gtaatcgcta tgcacgtggc cgccgttgat ctgccgccgc caacgatcgc
     1021 cgatttcgat ttcgccgttg cccgaggagg taccgttgcc gatgacgaag tggtaggcga
     1081 gaccattggt catccgccgc acgttgcggt ggtagtaatc gaagatgcgg gcgttgccct
     1141 gccgggtgcc gctgttatgc acgatgatga agcgccagcg ctcgcgcagg accggggcgc
     1201 gatcgatctc ccggcgcacc gagctggtga ggtaggtata gtgctttccg aaaaggtgga
     1261 acggcaggag ggaaaagccg cttggctttt ggtagccgga tttttcaatc acgacctgcg
     1321 cgggcgccgc tgaagcaccc ggcgccgctg aggcaggggg ccgcggcggc ggtgtaacgg
     1381 cgacggcgtg ggccggagtg gattcttcag gggtggggtg gcgcgcgggc gtttcctgcg
     1441 cggtcggctg cggggtgctc tcgggtgttt cctccggctc aggggtcggc ctgggggtgg
     1501 cggacttttt ctttttcgcg ggcggatgtt tggccggctg cggtttgtgg cgcggggtgg
     1561 gggattcctc cggggtgggc gagccgacgg tgcgcatttt ctcgcgtgac ttgaggaaga
     1621 gctccttctt ttcttcttcg gtgagttcgg cccgggtgag cgtgccggca aggctggcga
     1681 cgaggagcag cccgcaaaaa acgaactgcc agcggacgat gaaacgcggt cggatcaaag
     1741 cgcgagcagc gttaggccgg ctgtggctca cttgctgccg cttgctccgc gcgggctgcg
     1801 gcgtcttcgc tctcgcgccg caggatggcg tttaattttc caccgaggag caggaggagg
     1861 caggtgagat acatccaggt gaggagcatg atgagcgacg cgagcgcgag gtaggtcggg
     1921 ttgtagcgat cgtagcgcgc gacgtaaaac tggaagagtt tcgtcacggt catccaaccg
     1981 acggagaaaa agatcgcgcc gggcagggcc tgccggatgg tgagataatt ctgcggggtg
     2041 agcaggtaaa gggcgagggc gaggacgacg agggcgaggg cggtgaaggg aatgttgagg
     2101 gcggcgaccc attcctggag cgggatgcgc agttggaaat tgacctcggc gatgccggcg
     2161 agcgtctcgc cgaaaacggt caggttgaag gcgaaaataa tcgtgattcc aaaccagaag
     2221 aggagcagga gcgagatgat gtatttcgac caccaatggc ggtcctcggt cacgccatga
     2281 gtgcgactga gcgcgcgcga gatggtggcg atgaacatcg tgccgaggta aattccgagg
     2341 aagatacccg cggtggcgag cgctccgccg ctgccggtga cgatgacgtt ggccacgacg
     2401 ctatcgagga tttcctgcac tttcgggtcg ggcggcagga aggcgcggac gacaccgagc
     2461 attttgtgca gctttgccgg gtcggtcccg aagaggccga gcaggtgcca aaggaaaagg
     2521 agcccggggg cgagggtgaa aaggaattgg taggccagtt ggccggagag gcccatcgtc
     2581 tcgtcggtgg ccgtctccca gacgaggtgt cgtaccacgc ggccggtcaa gcggaggatt
     2641 ttcatcgtca cgcgaagttt actttgccgc tccgggattg ccattaaact ctccgccggc
     2701 ctcctggcct aacgaatgga gaaacgaatc ctgattttga ccgccggcta cggcgagggc
     2761 cacaatgccg cggcacgcgg gatacgcgac ggcctggcca cggtcgccgg agacgccgcc
     2821 atggccgagg tgcacgatct ttttgccgaa acctacgggg tgacaaacga ctgggtgcgg
     2881 aaggggtacc tcgcgctgat caaccgcgca ccgcgcgtct ggaagcggtt ctacgactgg
     2941 accgatcggc aggaggactt ttctgccagc atgcgctgga ttttccaggc gaagaaccgg
     3001 ctgagccaac tgctggtccg cttccagccg acggcggtgg tgtcggtttt tccggcctac
     3061 ccgcatctgc tggaggaaat cctgggcgat tccaggccgg cctttcggcg ggtcgtttgc
     3121 attaccgact ccattacggt gaacaaaatc tggtttcgct gcgcgagcga cctgtttctc
     3181 gcgcctaacg aacagacggc cgccgttctc gccgcgagcg gtgtgcctgc cgacaaggtg
     3241 cgggtgagcg gatttcccgt cacgccgaag ttcgcggagc tgggcgagtt gcggcaaccg
     3301 cccgcgccgg acagccggcg ccggctgctt tacgtgatca actccggcgg cctcgccccg
     3361 cccgaaatca tccgcggcct cgccctgccc gacgttcggc taaccgtgac ggtgggacgt
     3421 gacgagcggt tgcgcaagac ggtcgaagcg gtgcgcgcgg aagcggggcg cgacttcgag
     3481 atcgtcggct ggaccgatca attgccgcgg ctcatgctgg agagtcacct cctgatcagc
     3541 aaggcaggcg gcgcgacggt gcaggaaacg atcgcggcga agtgtccgat gatcatcaat
     3601 caaatcgtgc ccggccagga ggaaggcaac gcgcggctga tcgaggaaac gcgctcgggg
     3661 gttatcgcga cgacgccggc aacgattagc gcggcggtgc agcgggcgtt tgccgatgac
     3721 gcgcggttat ggcgggagtg gtcggagaac atcggccgga tc
//
LOCUS       DATMNS010000028         3310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2181742, whole genome shotgun sequence.
ACCESSION   DATMNS010000028 DATMNS010000000
VERSION     DATMNS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2181742"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VNW28_01110"
     CDS             <1..508
                     /locus_tag="VNW28_01110"
                     /inference="COORDINATES: protein motif:HMM:NF015748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HXA08547.1"
                     /translation="RGAGLRDRVYGPTFMRRCLEASPAPWKHYLLGGSVECVARLRAR
                     FSPPAQIVGARHGYFAPDEEAEILDEIKRLEPDFIWIGLGTPKQQDWIYRHKAAIQRG
                     VLFAVGFAFDVNAGMKPDAPAWMQRLGLTWLFRVGSEPGRLLTRYLRFNSLFLYYLAR
                     DAITSPRP"
     gene            483..1538
                     /locus_tag="VNW28_01115"
     CDS             483..1538
                     /locus_tag="VNW28_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08548.1"
                     /translation="MRSPLLVLEKTRGFVLVVVWALVLSAAAEEGPSSPSFIKGMTVS
                     CQTSGWEWATPEMAKTLDQLKSLGVNSFAIHPYAQIENDGHVRFRSGDDFRHISVPLD
                     WARERGLSVMLIPHIAYWGTKFLWRGEINFQTAEEWDRFFGDYQSWIVQMAKVAEAHG
                     AQTFCIGLEFTYAQKFDARWRQIIAAIREVYHGKLTYGGNWDSFQEVTFWDALDYIGV
                     LAYFPLTKSVDPSEAEIAAAWQARCAELTAYSKAHGDKKFLFVEIGYNVSARAAAEPW
                     AFQMGGDHAEEIQQRCIGVALDLPRHCPTIAGMYWWKWFPELPSHEEENYRLQTPAIK
                     ALIAKHWKEASPALGTR"
     gene            complement(1667..>3310)
                     /gene="mutL"
                     /locus_tag="VNW28_01120"
     CDS             complement(1667..>3310)
                     /gene="mutL"
                     /locus_tag="VNW28_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HXA08549.1"
                     /translation="IDAEARSIEVMIRRGGISLLRVTDDGIGMDRDDALLCLERHATS
                     KIRTADDLAVVTTLGFRGEALPSIASISRFRLTTREKEAVAGTEVIVSGGKIETVRDG
                     GEAPGTQIEVRSLFYNLPARRKFLRSENTETRNIEHQLNLQAIGHPEIAFSLVRDERV
                     VFQLPATKALRDRIRDLLGSELVARLLEVEPTKTRVRVSGFVGQAGMSRASRAQQFVF
                     VNGRAVESPVIGAALKEGYHTALMKGQFPVTFLFVELDPAEVDVNVHPAKREVRFRDP
                     AGVREAVVRAIQRTLESDRRSWQEQFRGPAASDGPVVRPSEALPRPNEDTQPVLPVEL
                     RVPAVTDRRFAPDEGRRPVAEHQFRILGILNKLYVLMENAEGLVLVDQHAAHERILFE
                     ELRRRMEEKGVPSQRLLLPQVIELAPRDAEWIERNLATLQKMGLGVEEFGRHTFKLES
                     LPPFLSGAEPGRLLQDVIDSLKSASNNSSPLRLGEEMIAKTVCRHAVKANDLLRQPEV
                     EKLIRDLLACELPYCCPHGRPTMIQISLGELEKKFGRKV"
BASE COUNT          601 a         1094 c          961 g          654 t
ORIGIN      
        1 gcgcggcgcc ggcctgcgcg accgggttta tggcccgact ttcatgcgcc gttgcctcga
       61 ggccagtcct gctccctgga agcattattt gttaggcggc tccgtcgagt gcgtcgcccg
      121 gctgcgcgcg aggttttcgc cgccggccca aattgtcggc gcccggcatg gctattttgc
      181 gccggacgaa gaagcggaaa ttctcgacga gatcaaacgg ctggaaccgg actttatttg
      241 gatcgggctc ggcacaccga aacagcagga ttggatctac cggcacaagg ccgcaatcca
      301 acgcggtgtt ttgtttgcgg tgggatttgc ttttgacgtc aacgccggga tgaaacccga
      361 tgcgcccgcc tggatgcagc gcctcggact gacctggctt ttccgcgtcg gctccgagcc
      421 gggccgattg ctgacccgtt accttcgctt caattcactt ttcctttatt atctggccag
      481 ggatgcgatc acctctcctc gtccttgaga aaacgcgcgg cttcgtgctc gtcgtcgtct
      541 gggccctcgt tctttcggcc gcggcggagg aaggcccgtc gtctccctcc ttcatcaagg
      601 gcatgaccgt ctcctgccag acttccggct gggaatgggc caccccggaa atggccaaga
      661 cgctcgatca actgaagtcg ttaggcgtaa acagcttcgc gattcatccc tacgcccaga
      721 tcgaaaacga cggtcacgtt cgcttccgca gcggtgatga cttccgccac atctcggtgc
      781 cgctcgattg ggcgcgcgag cgcggcctgt cggtgatgct cattccgcac atcgcctact
      841 gggggacgaa atttctctgg cgcggcgaaa tcaatttcca gaccgccgag gagtgggacc
      901 gcttctttgg cgattaccaa agctggatcg tgcagatggc gaaagtggcc gaggcgcacg
      961 gcgcccagac tttctgcatc gggctcgaat tcacctacgc gcagaaattc gacgcccgct
     1021 ggcgacagat aatcgcggcc atccgcgagg tttatcacgg caagctcacc tatggcggca
     1081 actgggacag cttccaggag gtgacttttt gggacgcact cgattacatc ggcgtgctgg
     1141 cctatttccc cctcacgaaa agcgtcgatc caagtgaggc cgagatcgcg gccgcctggc
     1201 aggcgaggtg cgcggagctg accgcttact cgaaagcgca tggcgacaag aaatttcttt
     1261 ttgtcgagat cggctacaac gtcagcgccc gcgcggcggc tgagccgtgg gccttccaga
     1321 tgggcggcga ccacgccgaa gaaatccagc agcgttgcat cggcgtggcg ctcgatctgc
     1381 cgcggcactg ccccactatc gccggtatgt attggtggaa atggttcccg gaattgccga
     1441 gccacgagga ggaaaattac cgactgcaga ctcccgcgat caaagccctg atcgcaaagc
     1501 actggaagga agcgtccccc gctttgggca cccgttagcc caaacgcaag cgagtgccag
     1561 aggaacgtca tcccgagcgt aaccgagggg cccggagcgt catcttccac ctccgatcac
     1621 gcgatccttc gacttcgctt gcgctccctt ccggatgacg aacccgctac acttttcgtc
     1681 caaacttttt ctccagctcc cctaacgaaa tctggatcat cgtcgggcgg ccgtgcggac
     1741 agcagtaggg cagctcgcag gcgagcaggt cgcgaatcaa cttttccact tccggttgcc
     1801 ggagcaggtc gttggccttg accgcgtgcc ggcaaaccgt cttcgcgatc atctcctcgc
     1861 cgagccgcag cggcgagctg ttattgctcg ccgatttcag actgtcgatc acgtcctgca
     1921 ggagccgacc tggctccgcc ccgctcagaa acggcggcag gctctccagc ttgaaggtat
     1981 gccttccaaa ctcttccacc ccgagtccca tcttttgcag ggtagcgagg ttgcgctcga
     2041 tccattccgc gtcgcgcggc gccagctcga tcacctgcgg gagaagcagc cgctgcgagg
     2101 gcacaccctt ttcctccatt cggcggcgca gctcctcgaa caggatccgc tcgtgtgcgg
     2161 catgctggtc gaccagcacg aggccctcgg cgttctccat caaaacgtag agcttgttca
     2221 ggatcccaag gattcggaat tgatgttccg ccaccgggcg gcgcccttcg tcaggcgcga
     2281 atcgacgatc ggtcacggcc ggtacgcgca gctcgaccgg caggaccggc tgcgtgtctt
     2341 cgttaggccg aggcagagct tcgctcggcc taacgaccgg cccgtcgctc gccgccggcc
     2401 cgcgaaattg ttcctgccag ctgcgccggt cgctctccag cgtccgctga atcgcccgca
     2461 cgacggcttc ccggactccc gccggatcac gaaaacgcac ctcgcgtttc gccggatgca
     2521 cattcacgtc cacctccgca ggatcgagct cgacgaagag aaatgtgacc gggaattggc
     2581 ccttcatcaa tgccgtgtgg taaccctcct tcagcgccgc gccgatgacc ggactctcga
     2641 cggctcgtcc attaacgaag acaaactgct gcgcccggct ggcccggctc atgcccgcct
     2701 ggccgacgaa cccgctcact cgcacccttg ttttcgtcgg ttctacctcg agcaaacgcg
     2761 ccaccaattc gctccccagt aaatcgcgaa tccgatcgcg cagcgccttt gtcgccggga
     2821 gttgaaagac gactcgctca tctcgcacca gagagaaggc gatctccgga tggccgatcg
     2881 cctgcagatt caactggtgc tcgatattgc gcgtctccgt gttttcggag cgaagaaact
     2941 tccggcgcgc gggcagatta taaaaaagcg agcgcacctc gatctgcgtc ccgggcgctt
     3001 ccccgccgtc gcgcaccgtt tcgattttcc cgccggagac aatcacctcg gttcccgcaa
     3061 ccgcctcctt ttcccgagtc gttaggcgaa aacgagagat gctcgcgatg ctcgggagcg
     3121 cttctccgcg gaagccaagg gtcgtcacca ccgcgagatc gtccgccgtg cggattttgc
     3181 tcgtcgcgtg gcgctccagg caaaggagcg catcgtcgcg gtccatgccg atgccgtcat
     3241 cggtcacgcg aaggagcgag atgccgccac ggcggatcat cacctcgatc gaacgcgcct
     3301 cggcatcgat
//
LOCUS       DATMNS010000029         8928 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_753003, whole genome shotgun sequence.
ACCESSION   DATMNS010000029 DATMNS010000000
VERSION     DATMNS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8928
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_753003"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..1191)
                     /locus_tag="VNW28_01125"
     CDS             complement(229..1191)
                     /locus_tag="VNW28_01125"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXA08550.1"
                     /translation="MAVQFRDYYETLGVSKAAGADEIKSAFRKLARQHHPDMAKPKEK
                     AAAEEKFKQINEAYEVLSDPEKREKYDQLGADWNQPGGFQPPPGWGRGGAGGGFRRAA
                     GDNGGVEFEFGGTGFSDFFEAFFGGGRGESAFGGGFGRRPATAERGQDVEADIMVTLE
                     EALHGSTRQVSLRRGGSEKVETYQVKIPRGVHEGQRIRLAGQGEAGARGGTHGDLFLR
                     VRIARHPDFAVEGSDLIHEVTLEPWLAVLGTEIEVSTLEGDRRLKIPPGTQSGQRFRL
                     RGRGLPTVAGARGNLYVVTQIAIPKKLSETERKLWEQLAALPRT"
     gene            complement(1332..1808)
                     /locus_tag="VNW28_01130"
     CDS             complement(1332..1808)
                     /locus_tag="VNW28_01130"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HXA08551.1"
                     /translation="MNSIPALTIENVASLDGILGDFLKQAEAELTVVIDRGGNVISQF
                     GDVSVMDVTIIAALAAGSFAATKELARRIGEVEFNALYHQGNGSHIFMNSVDDDTIMI
                     TVFGRRTTVGLVRFYSATTATAVSELLKSLPATGGHGFTFDAADVTAAPLFVDRPL"
     gene            complement(1805..2485)
                     /locus_tag="VNW28_01135"
     CDS             complement(1805..2485)
                     /locus_tag="VNW28_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HXA08552.1"
                     /translation="MSIINYASREIQFKIVYYGPALCGKTTNLAYIHERVSPANRGDL
                     VSLATAADRTLFFDFLPLNAVVIKGFVTKFQLYTVPGQVIYNATRQLVLRSVDGVVFV
                     ADSQWEKMEDNVESFRNLEENLAQQNISIDEIPYVLQYNKRDLANIAPINYLEFLLNN
                     RRKRAQSFEVTSSTGQNVFASLNAVSQMLLYKFSKQSDVTPTAAPPPVAGQAEPAAAP
                     KIVAPARV"
     gene            complement(2495..4561)
                     /locus_tag="VNW28_01140"
     CDS             complement(2495..4561)
                     /locus_tag="VNW28_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08553.1"
                     /translation="MNLSFLRYFKKDKAPANAVAVASAPVPPIEKPPSERFGKTVMPN
                     SSRVVGVESSRNFPVPAPLSGPTPTGPRTISLGGNGSMGVAPITPGGDRTIALSLGDL
                     VPHIPAGMLRPAAIDPQQRVTLKAADVERGMATGRPAVLLRAIYQQAPEFFASKVEEA
                     DETEVALPLDKVLEQFAAFQVRDDQICDEAIPHVETPFLKVTVEDGARFGTPVAPLRT
                     SAQPVVAVQPATAGAIAAAQPEAVAEIKPPPAPVRLPEPSKTSPVASGSSAKAPIRLD
                     QPPAASLIRAKISPNGTGVPATERVPASSGPPVPTPLAVSAAPVVPPAPAAPTRIPFK
                     ISPPSNDLRPTVLHAAKTKAEAEAIAFSSAGPRIQLPLGIILRGIPAFQLTGSIDEVP
                     AEAVIELPFAIIEPQLSLGKIAISPAQFQAAIPEKYRSLFKAEEAGTPVSLPLPQVLQ
                     NLPNESLQIRGDQEEVEIGETFETPFSQKMAEDAVRIKGSDGPIAKPAIRFAPLEPEL
                     VRAAPEKEPVPVAAPSAKRAVALAKPKPEPAPVKPIAEEVPAAPAGRNALQVALDTDE
                     TLTAKSVVDRVSHLPGVIACAIVFSDGLSLAGNIPAEYEAEALCALAPSIVRRISDQM
                     MGTNLGSLSGMTLYCAKAPVSFFAHGNICLAAIHSATEIAPEIRDRLGSIAQELARMY
                     ANQPND"
     gene            complement(4562..6274)
                     /locus_tag="VNW28_01145"
     CDS             complement(4562..6274)
                     /locus_tag="VNW28_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXA08554.1"
                     /translation="MRSKSFGERIADVLIEDGLLLPNQLEEAATIQKQQGGRLLKILT
                     DKQFVSDQDMAFSMGRCLNTPPINLSKVRVPEGILDLIPRDLAKAHKLVPIARLEKKL
                     FVAMADPTNVLAVDDLKRRVQMEIIPMITTERAVNEALSGVHSGQNMSDVLKQVADEA
                     AGDVEVQKQGREEIDLDRLATDSEDAPVIKIVNLILVQAIKEKASDIHLEPFQRSLKL
                     RYRIDGELIQAESPPKALQLAITSRIKILAGLNIAERRIPQDGRFRIKVMGKEIDLRI
                     SILPTSHGEKVVIRILDKSALAGSIETMGMDEETVRRFRKAIDAPHGMILVTGPTGSG
                     KTTTLYSVLQELNSPNYNLVTVEDPIEYELMGVNQVAARPDIGLDFASALRSILRQDP
                     DIVMVGEIRDNETADIAVKAALTGHQVLSTLHTNDAAGAIARLDDMGIEPFLIASSVI
                     MCCAQRLIRRVCVNCREEFVPEPEMFVRLGMEPTEGAVFYHGVGCDRCKSRGYSGRAA
                     IIEALTVSEAIRRLIIKRASAAVIKNQAITEGMKTLRMVGIDKALEGITTLEEVWRVT
                     AEDH"
     gene            complement(6286..7239)
                     /locus_tag="VNW28_01150"
     CDS             complement(6286..7239)
                     /locus_tag="VNW28_01150"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08555.1"
                     /translation="MVLAFYRLVLEHALSDEERNQILQTIEMFEVITQSQKDDYQSLE
                     ILKVAYQKLGKTAESLRISRRLAEAYHSVGSFSLALQECEAVLAVEPNAPEILAMLGE
                     IENRLQPSDQSNVDPSLEHSLIETPDYRASSAPTTASRLLESGGRNAGQARKRVVPQD
                     PDQGNDPLGKFLIVQQLFPEDEVKTALAAVKEANAAISGQTLATSLLDWLCKADDEKM
                     ERVLSALIDRTKFAYVPLEYYDVDRQVVRMLPDELTINRLYVPFDLISRTIMVAVCNP
                     FDSAGRDAVQQSLDYTVTWYLAKPATVIKTLQEIHRLEARV"
     gene            7238..8368
                     /locus_tag="VNW28_01155"
     CDS             7238..8368
                     /locus_tag="VNW28_01155"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXA08556.1"
                     /translation="MPMPRRRMGLAKNHGICAARKRAATGAEHRGLTRGRGNRPTAAV
                     RLVDKCGDTPIDFPDANLVRFAEASGIDPVLTLDRHGFLTYRFGKSRRFKLVFDQCLR
                     PRSRTSASPNPEPFILDSLVPLADPLASFRAFPTGSHQAFQLFSFQHLRFLCYNRLPI
                     PNPLAVPPTSPNQRPRYAFTLMEMLVVIVIIAIVLAVLIPALSPSSARALEGDSRNFA
                     AQLDNARMMALAKRTKTRVLIAATNDWGTDFSWRAYVLTSFDSTTGNWLQQSKFFRLS
                     QSTTFDSATGIVATRSSMTTSVVKASNATPTPAPSPFTGAYVEFNPTGSTNLDPSATP
                     EIVRIQDGFVPATGPSPTPVRKNQTLHTDVSIDPASGGMKLQ"
     gene            8365..8802
                     /locus_tag="VNW28_01160"
     CDS             8365..8802
                     /locus_tag="VNW28_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXA08557.1"
                     /translation="MTAPKRIIPASAFSLVEVVVAIGIVSFAVLSTFGLLSVATDTNR
                     RARDEQSAAQLAENEFQRIRSLSSANIPATTYVTRYYDTSSNDLGTTLTNAAIYQLQI
                     AIVTPAAPAPADRIFNAEVHYPANAAPGNQNIVRFTTLMNLPP"
BASE COUNT         1647 a         2767 c         2652 g         1862 t
ORIGIN      
        1 cgctcgaagt gccaaagtcc tccacttcgt gccctaacga gatgagcagt tccttgattt
       61 tttctttgta acgaaagccg gcgtgatcgg agccgagagc gattttcatt tcgtcgtttt
      121 ataggaacgc ggcggccctg tcgaggcttc accaaggtgg gaggggcctc tgcgcctcga
      181 taggcagcga cgtgcgatct gatcaggggc agaggcccta cccacattct aggttcgggg
      241 gagcgcggcg agctgttccc aaagtttgcg ttcggtctcg ctcaactttt tcggaatggc
      301 gatctgcgtg accacgtaaa gattaccgcg cgctcccgcc acggtcggca gcccgcggcc
      361 gcgcagacgg aagcgctggc ccgactgcgt gccgggggga attttcaaac gtcgatcgcc
      421 ttccagggtc gacacctcga tctccgtgcc aagtaccgcc agccatggct ccagcgtgac
      481 ctcgtgaatc agatcgcttc cttccaccgc aaagtcggga tggcgcgcga tgcgcacccg
      541 cagaaacagg tctccgtgtg tgccaccccg cgcgcccgct tcgccctggc ccgccagtcg
      601 gatgcgttgg ccttcgtgca cgccacgggg gatcttgacc tggtaggttt ccactttctc
      661 ggaaccaccc cgccgcaacg agacctggcg ggtggaaccg tgcagcgctt cttccagggt
      721 caccatgatg tcggcctcga catcctggcc gcgctccgcg gtcgccggtc gccggccaaa
      781 gccgccaccg aacgctgact ctccccttcc gccgccaaag aacgcttcga aaaagtcgct
      841 gaaaccggtc ccgccaaatt caaactccac gccgccgttg tcgccggccg cccgccgaaa
      901 cccaccgccc gctccgccgc ggccccaacc cggtggcggt tgaaacccgc ccggctgatt
      961 ccaatccgcg cccagctgat cgtatttttc ccgcttctcc ggatcgctca ggacttcgta
     1021 ggcttcgttg atctgcttga atttctcctc ggccgcggcc ttttccttcg gcttggccat
     1081 atcgggatga tgctggcgcg cgagcttgcg aaaggcgctc ttgatttcat cggcgccggc
     1141 tgctttcgaa acgccgagcg tctcgtaata atccctgaat tgaaccgcca tagattgtgc
     1201 ggttgaattt cccgcatcgc ccggattgcg caacccggct acgccggtag acgcttcgct
     1261 ctgcgcagcg cggccgcgcg catagccgaa gagaaccggg acgcctaacg acgctctctt
     1321 ccgcgctgcg tctacaacgg acgatcgacg aagagcggcg ccgcagtcac gtcggcggca
     1381 tcaaaagtga acccatggcc cccggtggcg ggcagcgact tcaacaattc cgagaccgca
     1441 gtggcggtcg tggcggaata gaaacggacg aggcccaccg tggtgcgccg cccgaagacc
     1501 gtgatcatga tcgtgtcgtc gtcgaccgag ttcatgaaaa tatggctgcc gttgccctgg
     1561 tgatagagcg cgttgaactc gacctcgccg atccggcggg ccagttcctt ggtcgcggcg
     1621 aacgaacctg cggcgagcgc cgcgataatc gtgacgtcca tcaccgagac atcgccgaac
     1681 tgcgagatca cattcccgcc gcgatcgatc accacggtta attccgcttc cgcctgcttc
     1741 agaaaatcac cgaggatgcc gtcgaggctg gcgacgttct cgatcgtgag cgccgggatg
     1801 ctgttcatac tctggccgga gccacaattt tgggcgccgc cgccggctcc gcttgtccgg
     1861 ccactggggg aggcgcggcg gtcggcgtga cgtcactttg cttgctgaat ttgtagagca
     1921 gcatttgcga gacggcgttg agcgaggcga agacattttg gccggtgctc gaagtcacct
     1981 cgaaactttg cgcccgcttt cggcgattgt tcaaaaggaa ctcgaggtaa ttgatcggcg
     2041 cgatgttcgc caggtcgcgc ttgttgtact gcaagacgta ggggatctcg tcgatcgaga
     2101 tgttctgctg ggccagattt tcctccaggt tacggaaact ctcgacgttg tcctccatct
     2161 tctcccactg cgagtcggcc acgaaaacca cgccatcgac actccgcagg acgagctggc
     2221 gggtggcgtt gtaaatcacc tgaccaggca cggtgtagag ctggaatttg gtgacaaagc
     2281 ctttgatgac cacggcgttc aacgggagaa aatcaaagaa cagagtgcga tcggcggcgg
     2341 tggcgagtga aacgagatcg ccgcggttgg ccgggctgac ccgctcgtgg atatacgcga
     2401 ggttggttgt cttgccgcag agggcgggac cgtagtacac gatcttgaac tgaatttccc
     2461 ggctggcgta gttgatgatg gacatggcgt tttcttagtc gttaggctga ttggcataca
     2521 tccgcgcaag ctcttgcgcg atgctgccaa gtcggtcacg gatttcgggc gcgatttcgg
     2581 tggccgaatg gattgccgca aggcagatat tgccgtgcgc aaaaaaacta accggcgctt
     2641 tcgcgcagta aagcgtcatg ccggagagcg atccgagatt ggttcccatc atctggtcgc
     2701 tgatccgccg cacgatcgag ggcgccagcg cgcagagtgc ctcggcttca tattcagccg
     2761 gtatgtttcc cgccagactg aggccgtcgg agaaaacgat cgcgcaggcg atgaccccgg
     2821 gcaggtggct gacgcggtcg acgactgatt tcgcggtcag tgtttcatcg gtatcgagtg
     2881 cgacctgcag ggcgttgcgt cctgccgggg ccgcgggcac ctcttctgcg atcggcttga
     2941 ccggagccgg ttcgggcttc ggtttagcca gggcgacagc acgtttcgcg cttggagcgg
     3001 cgacgggcac gggctctttt tccggcgcgg cccggaccag ttcgggctcc agtggagcga
     3061 aacggatggc aggtttcgcg ataggaccgt ccgaaccttt tatccgcacg gcgtcctcgg
     3121 ccattttctg gctgaacggg gtctcgaaag tctccccgat ttcgacttcc tcctgatcgc
     3181 ctcggatctg caaggactcg ttaggcagat tttgcaatac ctgcggaagc gggagtgaga
     3241 ctggcgtccc ggcctcctcg gccttgaaga gcgaacggta tttttccggt attgccgcct
     3301 ggaattgggc gggcgaaatg gcgattttgc ctaacgacag ttgcggctca ataatcgcga
     3361 agggcaactc gatcaccgcc tctgccggca cttcgtcgat cgacccggtc aattggaagg
     3421 caggaatccc gcggaggatg atgcccagcg gcagctgaat gcgcggaccc gcgctcgaaa
     3481 acgcgatcgc ctccgcttcg gctttcgtct tcgcggcgtg caaaacggtc ggacgcaggt
     3541 cattggaagg tgggctgatc ttaaagggaa tccgcgttgg cgccgccggg gcgggaggca
     3601 cgacgggcgc ggcagacaca gctaaaggcg taggaacggg cggcccactt gaggctggaa
     3661 ctctctcagt ggcgggcaca cccgttccgt tcggagaaat cttcgcccta attagcgaag
     3721 ccgcgggcgg ttgatcgagt cggatgggag ccttggccga ggagccgctg gcgactggac
     3781 tggttttgct tggctcggga agccggactg gcgcgggtgg cggtttgatt tcggcgacgg
     3841 cctcgggttg tgccgcggcg atcgcccctg cggtcgccgg ttgcaccgcc acgacgggtt
     3901 gtgcgctcgt ccgcagcgga gccacaggcg tgccgaaacg cgcgccgtct tcgaccgtca
     3961 ctttgagaaa gggcgtctcg acatgcggga tcgcctcatc gcaaatttga tcgtcccgca
     4021 cttgaaaggc ggcaaattgt tcgagcacct tgtccaacgg caaggccact tcggtctcgt
     4081 cggcttcctc cactttgctg gcgaaaaatt cgggcgcttg ttgatagatc gcccgcagga
     4141 gcaccgccgg acgaccagtg gccatcccgc gctcgacgtc ggccgccttc aaagtaaccc
     4201 gctgctgcgg gtcgatcgcc gccggccgca gcattccggc cgggatgtgc gggaccagat
     4261 cgcccagaga cagggcgatg gtccggtccc caccgggcgt gatcggcgcg acgcccatgg
     4321 aaccgtttcc gcctaacgat atcgtccgcg gcccagtcgg cgtcggacct gagagcggag
     4381 ccggaaccgg aaaattccgc gaggattcca ccccgacgac gcgcgaggag ttgggcatga
     4441 ccgttttccc gaatcgttcg gacggcggtt tctcgatcgg cggaacgggc gcgctcgcga
     4501 ccgcgaccgc attggccggg gccttgtcct tcttaaaata gcgcagaaac gataaattca
     4561 tttagtgatc ctcagcggtg acgcgccaga cttcttcgag tgtggtgatt ccctctaaag
     4621 ctttgtcgat gccaaccatg cgcaaagttt tcatcccttc ggtgatagcc tggttcttga
     4681 tcaccgcggc ggacgcacgc ttgatgatca agcggcgaat cgcctcgctc accgtcagcg
     4741 cctcgataat cgccgcgcgt ccggaatatc cgcggctctt gcaacgatcg cagcctaccc
     4801 cgtgatagaa gacggcccct tccgtcggct ccatcccgag gcgcacgaac atttccggct
     4861 cggggacaaa ttcttcgcgg caattgacac acacccgcct gatgagtcgt tgcgcgcagc
     4921 acatgatgac cgaactggcg atcaggaaag gctcaatccc catgtcgtcg agtcgcgcga
     4981 tcgcgccggc cgcgtcgttc gtgtgcagcg tgcttaagac ctggtgaccg gtaagggccg
     5041 ctttcaccgc gatgtccgcc gtctcgttgt cacgaatttc gccgaccatc acgatgtccg
     5101 gatcctgccg gagaatcgag cgcagcgcgg acgcaaaatc gaggccgata tccggccggg
     5161 ccgccacctg gttcactccc atcagctcgt actcgatcgg gtcctcgacc gtcacgaggt
     5221 tgtagttggg gctgtttaat tcctggagca cggaatagag cgtcgtcgtc ttgcccgaac
     5281 cagtcggacc ggtgacgagg atcatcccgt gcggcgcgtc gattgccttg cggaaacgcc
     5341 ggaccgtttc ctcgtccatt cccatcgttt cgatgctgcc ggcgagggcc gacttatcga
     5401 ggatacgaat gacgaccttc tcgccgtgcg aggtgggcag gatcgaaata cgcaggtcga
     5461 tctctttccc catcaccttg atgcggaacc ggccgtcctg cggtatgcgc cgctccgcga
     5521 tgttgagccc ggcgagaatc ttgatccgcg aagtgatcgc gagctggagc gctttgggcg
     5581 gcgactccgc ctggatcaat tcgccatcga tgcggtaacg caatttcagg ctccgctgga
     5641 agggctcgag gtgaatatcc gacgcctttt ccttgatcgc ctggactagg atcaggttca
     5701 cgatcttgat caccggcgcg tcttcgctgt cggtcgcgag acggtcgaga tcgatctcct
     5761 cgcggccctg cttctgcact tcgacgtcgc cggccgcttc atccgcgacc tgcttgagca
     5821 cgtcgctcat gttctggccg ctgtgcacgc cgctgagcgc ttcgttcacg gcgcgctccg
     5881 tcgtgatcat cgggatgatc tccatctgca cgcggcgctt cagatcgtcg accgcgagca
     5941 cattggtcgg gtcggccatc gcgacgaaca gctttttctc cagccgcgcg atcggcacga
     6001 gcttgtgcgc tttcgccagg tcgcgcggga tgagatcgag aatgccctcc ggcacgcgca
     6061 ccttcgacaa attaatcggt ggcgtattca ggcagcgccc catgctgaag gccatgtcct
     6121 gatcgctgac gaattgtttg tcggttaaaa tcttgagcag ccgcccgccc tgctgtttct
     6181 ggatggtggc cgcttcctcg agttggttgg gaaggaggag gccgtcctcg atcaggacat
     6241 cggcgatgcg ttctccgaaa gatttgcttc tcattctcgc gcgggctaaa ccctcgcctc
     6301 cagtcggtgg atttcctgca acgtcttgat caccgtcgcc ggcttggcga gataccacgt
     6361 gactgtgtaa tcgagcgatt gctgcacggc gtcgcgtccc gcggagtcaa agggattgca
     6421 gaccgccacc atgatcgtgc ggctgatcaa atcgaagggc acatagaggc ggttaatcgt
     6481 cagctcatcg ggcaacatcc gcaccacttg gcgatcgacg tcgtaatatt ccagcggcac
     6541 gtaggcaaac ttcgtccggt cgatcaaggc cgagaggacc cgctccattt tttcgtcgtc
     6601 ggccttgcag agccagtcga gcaacgacgt cgcgagcgtc tgaccgctga tggcggcatt
     6661 cgcttctttg accgcggcga gcgcagtctt cacttcgtcc tcgggaaaca actgttggac
     6721 gatgaggaat ttgccgagcg gatcatttcc ctggtcggga tcctgcggca cgacgcgctt
     6781 gcgtgcctgg ccggcgttgc ggccgccgga ttcgagcaac cggctggcgg tcgtcggcgc
     6841 ggaactcgct cgataatcgg gagtctcgat gagagagtgc tccagactgg gatccacgtt
     6901 actctggtcg gacggctgca gccggttttc gatttcgccc agcatcgcga ggatttccgg
     6961 ggcgttcggt tccaccgcca gcaccgcctc acattcctgg agcgcgagag aaaaagagcc
     7021 gacactatga taagcctccg cgaggcgtcg cgaaatcctt agcgactcgg cagttttgcc
     7081 gagcttctga tacgcaacct tcaggatctc caacgactga taatcgtcct tctgcgattg
     7141 agtgattact tcgaacatct caatcgtttg gagaatctgg ttacgttcct cgtctgatag
     7201 cgcgtgttcc agcacgaggc ggtaaaaagc tagaaccatg ccgatgcccc gtcgtcggat
     7261 gggattagca aaaaatcatg gcatctgcgc cgcacgaaag cgggccgcga ccggcgcgga
     7321 gcatcgcggt ctcacacgtg ggaggggaaa ccgtccaacc gccgccgtgc gactggtgga
     7381 caaatgcggc gacaccccga tagatttccc ggacgccaac ctcgtccggt tcgcagaagc
     7441 gagcggcatc gacccggtgc tgaccttgga ccggcacgga ttcctgacct atcgattcgg
     7501 caagagtcgc cgattcaaat tggtcttcga ccagtgcctc cgacctcgat ctaggacctc
     7561 cgcctctccg aaccccgaac ccttcatcct tgattccctc gtcccgctcg ctgacccttt
     7621 ggcttcattt cgcgcgttcc ccacgggctc ccatcaagca ttccagcttt tcagctttca
     7681 gcatcttaga ttcctttgct ataaccgcct gccgattcca aatccactcg ccgtgccacc
     7741 aacgtcaccc aaccagcgcc cgcgctacgc ctttacgctg atggaaatgt tggtggtgat
     7801 tgtgatcatc gcgatcgtat tggcggtttt gatcccagcg ctttcaccga gcagcgcccg
     7861 cgccttggaa ggtgactcaa ggaacttcgc cgcccagctc gataacgcac gcatgatggc
     7921 acttgcgaag cggaccaaaa cacgagtcct gatcgccgcc acgaacgatt ggggcaccga
     7981 ctttagttgg cgagcttacg tgctcacaag tttcgatagt acaactggca actggcttca
     8041 acagagcaaa ttttttcggc tatcgcaatc gaccaccttc gattccgcca caggcattgt
     8101 tgcaactcgg agttcaatga ctacctctgt ggtcaaagct tcaaacgcca cgccgactcc
     8161 ggcgccgagt ccttttaccg gcgcgtacgt tgagtttaac ccgactggca gcacgaacct
     8221 cgacccgagt gcgacgcctg aaattgttcg tattcaggat gggtttgttc ctgcgacagg
     8281 accgtcgcct acaccggtac ggaagaacca gacgcttcac actgacgtct cgatcgaccc
     8341 ggcgagcggc ggcatgaaac tgcaatgacc gcgccgaaac gcatcattcc agcctcggcc
     8401 ttctcgttgg tcgaggttgt cgtcgccatc ggcatagtca gttttgcagt tctctcgacc
     8461 tttggcctgc tctccgtcgc gacggacact aatagaaggg cgcgcgatga acaatcggca
     8521 gctcaactgg cggagaatga atttcagcga atccgctctc tgagttcagc caacatcccc
     8581 gcgactacct acgtgactcg ctattacgac acctcatcga atgatctcgg aacgaccctt
     8641 accaacgcgg cgatttacca gttgcaaatt gccatcgtga cgccggccgc gccggcgccg
     8701 gcggatcgca tctttaacgc ggaagtccat tatccggcca atgccgcgcc ggggaatcag
     8761 aacatcgttc gcttcaccac cctgatgaat cttcctccat gaggcgctgc agggctttca
     8821 cgttggtcga attgctcgtt gccctcgcgc tcaccgtcat tctcgtcttg cttcttacga
     8881 gtgtcgtagc agcgacgctg ggcgcttgga gtcagggcag aaatcgcc
//
LOCUS       DATMNS010000030         6745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3957379, whole genome shotgun sequence.
ACCESSION   DATMNS010000030 DATMNS010000000
VERSION     DATMNS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3957379"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VNW28_01165"
     CDS             <1..233
                     /locus_tag="VNW28_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08558.1"
                     /translation="KALLLAGERTQIVQVNLAAEDIYSELRQIAQDINNSLRATTVDA
                     EFTRLPDAPNPAFGSAQGFALGMDSASHKPPQ"
     gene            complement(975..1196)
                     /locus_tag="VNW28_01170"
     CDS             complement(975..1196)
                     /locus_tag="VNW28_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08559.1"
                     /translation="MAPINSGGHLKTSERALRRLPKKKVQRKGKTSLKKDVEAGEEPT
                     RPAVGTLSRTKSLLLAALRVWELKNGIRD"
     gene            1283..2326
                     /gene="sppA"
                     /locus_tag="VNW28_01175"
     CDS             1283..2326
                     /gene="sppA"
                     /locus_tag="VNW28_01175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HXA08560.1"
                     /translation="MPLRFFGDFIPVMANRKLGCLSIFLFVALCASVLVNFALAAIAF
                     QHFEGVRRDEELRPRFREMIVERGTRGSENKIAVITLRGLISTGVAGNVGDSMVDDLR
                     WQLEQARNDDEVRAVVLEVDSPGGEVTASDMIYNAVVKTRAKKPVVVYMESLAASGGY
                     YVSCGGKYLMANETSITGSIGVIIQTLNYEQLFNKIGLASVVFKSGKFKDMLNGARPI
                     TPEERDYIQSFVMKTYDKFLGIVAKERNLPEDALRNGIADGRILSGRDALENKLIDGL
                     GQIEDAYAKAKELGHAPGAKIVKYGPPFNLGRFLRMLSKSGDTKIELSLPGQFSPQLQ
                     SGRAYFLPSFYAP"
     gene            2342..3130
                     /locus_tag="VNW28_01180"
     CDS             2342..3130
                     /locus_tag="VNW28_01180"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXA08561.1"
                     /translation="MFDSLINSLLVVAGLYVYLSLYRQVVGRQDGAAALGERQLGLPE
                     ALAAALLIGWFALNVVAAAKMEQAAFQTRDLLVTGGFSLAVICILTALLQLRGFSVLS
                     LGGFSRLGFGRIVSTAAVLLFAAYPLLFAADGISRWLLGGGSSKQQIVEMFTGSETLQ
                     QRVLIIVLAITIAPLAEEFIFRFFLYGVLKRYLGRGPGLLLNALLFAAVHVHLPSFGP
                     LLVLGGCLTLAYEWSGSILVSMTMHALFNFLSLSALAFPNLIPQ"
     gene            3127..4035
                     /locus_tag="VNW28_01185"
     CDS             3127..4035
                     /locus_tag="VNW28_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HXA08562.1"
                     /translation="MKLRELGEERLVAQLVARCKTGRDVIAGPGDDCAVVAAPGEGEL
                     LLLKTDCVVEGVHFRPNEKPAAVGGKAMARALSDFAAMSGRPQFALVTLLAPAGREAR
                     WPREIYRGLRRVADRFAVAIVGGETSQTEGPAVVSVSAVGTVEKDRWVSRAGGKVGDV
                     LFVTGKLGGSIRKKHLNFIPRIEEARWLTAHFRIHAMMDLSDGLGADLPRLAAASGVG
                     FEVDEKALPRTRGCSIAEAINDGEDYELLFALSPNDAVSLQKRWRKKFPRLSLRCIGR
                     LTRHSFRHRRITRHSFHGYLHFHERG"
     gene            4028..4423
                     /gene="tsaE"
                     /locus_tag="VNW28_01190"
     CDS             4028..4423
                     /gene="tsaE"
                     /locus_tag="VNW28_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HXA08563.1"
                     /translation="MAETIAFARTWAQALVPNDVIALVGDLGAGKTHFVKGLLQGLGS
                     AEEATSPTYTLVHEYQGGRLPLFHFDFYRLNQLPEIAEIGFDDYLEENGIAVIEWADR
                     FPQVLPQRTRWVRIDVPSASVRVIAEEEK"
     gene            4420..5013
                     /gene="tsaB"
                     /locus_tag="VNW28_01195"
     CDS             4420..5013
                     /gene="tsaB"
                     /locus_tag="VNW28_01195"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXA08564.1"
                     /translation="MKVLALELSSSLGSVAFLDETGAGQAQIFPADRKDSGSFYENLQ
                     AIYRNCGAPEVIVVGLGPGSYAGTRIAIATALGLQAASGARLVGLPSVCAYEPDEYVV
                     VGDARRNSFFFAHVVRGRCVEGPTLATEEELRAKLSSWPHQPIFAAQPLPQFAKISVA
                     HPSARQLAELALQVGFDPGQTLEPIYLREPYITIAKK"
     gene            5010..6107
                     /gene="alr"
                     /locus_tag="VNW28_01200"
     CDS             5010..6107
                     /gene="alr"
                     /locus_tag="VNW28_01200"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXA08565.1"
                     /translation="MNSLGTERCWAEVDCAALRHNAGVVRERVGPQPSLLAVVKADGY
                     GHGVVEVAHALRDDAELFGVANLREALVLREGGILHPVLILGPALPEERAAIKARGFI
                     ASVSSLAEALAFEGNAAGINFVIDTGMGRMGCTPEDALAELEKIGRHPDLILHSVSTH
                     LPAADEDVVFTEKELEQFDAFVRQMRFQIPGSYKVHVLLSAGILGFTHHRYDIVRAGL
                     MLYGSAPLPHEQKVLRPALTLKARIALLRDLPAGSSVSYGRTFITARPTRVATISAGY
                     ADGYPRSISNRDGTVLIGGRRCPVLGRVTMDLTMVDVTALPEVRVGDEVVLIGKQGAE
                     EILAAEVAERAGTIAWEIFTGIGSRVRRVYL"
     gene            6194..6556
                     /locus_tag="VNW28_01205"
     CDS             6194..6556
                     /locus_tag="VNW28_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08566.1"
                     /translation="MFQIIFNQLSAAEMAALPKTLQLELLAEFQILPEDLDHLDARRF
                     GVIEREGKKLHRYRAKDYRIYFERSPEGVTVHRVLHKNTLRDFLFRSKLPVAEDDQLG
                     KTREFWKLIEQGERTRKT"
BASE COUNT         1251 a         1987 c         2126 g         1381 t
ORIGIN      
        1 ataaggcgct cctgcttgct ggcgaacgta cccaaatcgt tcaagtcaat ttggctgccg
       61 aagatattta ctccgaattg cgccagatcg cccaggacat caacaatagc ctccgggcca
      121 ctaccgtcga tgctgagttt accaggctgc cggatgcccc caatcctgcg tttggttctg
      181 ctcagggctt tgctcttggg atggattcag ccagtcacaa accaccccaa taaaaagcag
      241 agaaaagttc tacggtcaaa agattttttg atgcgaaacg ttgcaaagct gtgcaacgat
      301 ccagcgccat tctcccctcg tgaaatgcga ttttgcaacg ctgtgcaaaa cagtgcgaat
      361 tcgttaggct caattagaaa tccgatgctc tatccggctg agctatgggc gctttacccg
      421 cgacctaatc gcgtcgggcg gggctgcaat cggcgctgtt tgcgacgccg ccttccgacc
      481 tgcgaggcag accgggaaac aagagttgcg ttacctgacc gatggtagcg cgccttcccc
      541 aacacgactc ggatggcgga caaagggcga aggcaacgaa ggaggcgccc ggctgcgcga
      601 ggcgcgtcgc gactgcctga gccgaaaccg gtccgcgggg agggggcagg ctgctcccgg
      661 agcgcaatat accgaagatg cccaaagcgg cgatgctaac aatgataagg tagatccgcg
      721 cggcgcgggt catggttaga aaaagagtcg aggcggcgga ctcgaaattt tgggtccgac
      781 tttactgcct cctatggcaa aaaccgttct tgcagtccac gatattcttg gtgcaagggg
      841 atagcgcggt catgaactga ggaacaagcg cgcaaccgct ctcccgagcg aagcgctgcg
      901 ccaaacattg tattcctgag ccgaaacagc cggctctccg tgccggcaaa aaatgttcga
      961 tcgccgttgc tatcctagtc gcgaatcccg ttcttcagct cccaaacgcg caacgcggcc
     1021 aaaagaaggc tcttcgttcg ggataacgtt cccaccgccg gtcgcgtggg ttcctcgccc
     1081 gcctcgacat ccttcttgag agaagtcttt cccttgcgct gcactttttt cttcggcaga
     1141 cggcgaagtg ctctctcgct ggtcttgaga tgcccgcctg aattgatcgg tgccataatg
     1201 ttaccccatg taaaaagcag cttccaagcc atccccagtc ccgccccgtt tgcgaggcgt
     1261 tctgggcggg cggaaaacgc ttgtgcctct gcggttcttc ggcgatttca tcccggtcat
     1321 ggcgaatcga aaactgggct gtctaagtat ctttttattc gtcgctctgt gcgccagcgt
     1381 gctggtaaat tttgccctcg cggcgatcgc ctttcagcac tttgagggcg tcagacgcga
     1441 cgaggaactc cggccgcgtt ttcgcgagat gatcgtcgag cgcgggacgc gggggagcga
     1501 gaacaagatc gccgtcatca cgttgcgcgg cctcatcagc acgggcgtcg ccggcaacgt
     1561 gggcgactcc atggtcgacg acctgcgctg gcaactcgag caggcgcgga acgacgacga
     1621 ggtgcgcgcc gtcgtgctgg aggtcgattc gcccggtggc gaagtgaccg cctccgacat
     1681 gatctacaac gcagtcgtca agacgcgggc gaaaaaaccg gtcgtcgttt acatggaatc
     1741 gctcgccgca tccggcggct attacgtttc ctgtggcgga aaatatttga tggcgaacga
     1801 gacgagcatc acgggcagca tcggcgtgat tatccagacg ctgaattacg agcagctctt
     1861 caacaagatc gggctcgctt cggtggtttt caaaagtggg aagttcaagg acatgctcaa
     1921 cggggcgcgg ccgatcacgc cagaggagcg cgattacatc cagagcttcg tgatgaagac
     1981 ctacgacaag tttctcggga tcgtcgcgaa agagcgcaat ctgccggagg atgcgctgcg
     2041 caacggcata gccgacgggc gcattctttc gggccgcgac gcgctcgaga acaagttgat
     2101 cgacggtctg ggccagatcg aggacgccta tgcgaaagcg aaggaactcg gccacgcccc
     2161 cggtgcgaag attgtaaagt atggcccgcc cttcaatctc gggcgctttc tccggatgct
     2221 gagcaagagc ggcgatacca aaatcgagct ttcgctcccc gggcagttca gtccgcaatt
     2281 gcagagcggg cgcgcctatt ttctgccgag tttctacgct ccatagccgc gccgccgcga
     2341 aatgttcgac agcctgatca actcgctgct cgtcgtcgcg ggcttgtatg tttatctctc
     2401 gctctaccgg caggtcgtcg ggcgccagga cggcgcggcg gctttaggcg aaaggcaact
     2461 tggtctcccg gaggcgctcg ccgccgcgct cctcatcggc tggtttgcgc tcaatgtggt
     2521 ggccgctgcg aaaatggagc aggccgcttt tcagacgcgt gatcttctcg tgaccggcgg
     2581 tttctcgctg gcggtaattt gcatcctgac tgcgctcctg cagttgcgcg gcttcagtgt
     2641 cctgtcgtta ggcggattct cccggcttgg ttttgggcgg atcgtcagca ccgccgccgt
     2701 tctccttttc gccgcttatc cgctcctttt tgcggccgac ggcatctcgc gctggctgct
     2761 cggcggcggt tcgagcaaac agcagatcgt cgaaatgttc accggctcgg aaacgttgca
     2821 gcagcgcgtc ctcattattg tgctcgcgat caccatcgct ccgctcgcgg aggaatttat
     2881 ttttcgtttc ttcctctacg gcgtcctcaa gcgctatctt gggcgcgggc cgggtttgct
     2941 cctgaacgcg cttctcttcg ccgcggtgca cgtgcacctc ccgagctttg gaccactcct
     3001 cgttctcggc ggttgtttga ccctggccta tgaatggagt ggctccatcc ttgtctcgat
     3061 gacgatgcac gcgctcttca atttcctttc cctcagcgcg ctcgccttcc ccaatctgat
     3121 cccgcaatga aattgcgcga gttgggcgag gagcggctgg ttgcgcagct cgtggcgcgc
     3181 tgcaaaacgg gccgggacgt gatcgccggc ccgggagacg attgcgccgt cgtggccgcg
     3241 ccgggcgagg gcgagctgct cctcctcaag acggattgcg tggtcgaagg cgtgcatttt
     3301 cggcctaacg aaaaaccggc ggcagtgggc ggcaaagcga tggcccgcgc cctgagcgat
     3361 tttgccgcga tgtccggccg gccgcagttc gcgctcgtta cgttgctcgc gccagccggg
     3421 cgggaggcgc gctggccgag ggaaatctat cgcggattgc gacgggtcgc cgatcgcttt
     3481 gcggtcgcga ttgtcggtgg ggaaacgagc caaacggagg gcccggccgt cgtttccgtc
     3541 agcgccgtcg gcacggtcga gaaggatcgt tgggtttcgc gtgccggcgg aaaagtcggc
     3601 gacgtgcttt tcgtgactgg aaaattgggc ggatccatcc ggaaaaaaca tctgaatttc
     3661 atcccgcgca tcgaggaagc tcgctggttg accgcccact tccgcatcca cgcgatgatg
     3721 gacctgagcg acggcctcgg cgccgatctt ccgcgcctcg ccgcggcgag cggagtcggc
     3781 ttcgaggtgg atgagaaggc actgccccgc acgcggggct gctcgattgc cgaagcaatc
     3841 aacgacggcg aggactacga attgctcttc gcgctttcgc ctaacgacgc cgtgtcgttg
     3901 caaaaaaggt ggcgcaagaa gttcccccgc ctttcactca ggtgcatcgg ccgccttacc
     3961 cgccactctt tccgccaccg gcggatcact cgtcactcct tccatggcta ccttcacttc
     4021 cacgagcgtg gctgagacga tcgctttcgc gcgcacctgg gcgcaggcac tcgtgccaaa
     4081 tgacgtcatc gcgctcgtcg gcgacctggg tgcgggcaaa acacatttcg tcaaagggct
     4141 gctccagggt ttgggaagcg cggaggaagc gaccagcccg acctataccc tcgtccacga
     4201 atatcagggc ggccggctcc cgcttttcca tttcgatttt taccggctaa atcaactgcc
     4261 ggagatcgcc gagatcggat tcgacgatta cctcgaggaa aacggcatcg cggtgatcga
     4321 atgggcggat cgctttccgc aggtcctgcc gcagcggacg cgctgggttc ggatcgacgt
     4381 tccgagcgcg tcggtccgtg tgattgccga ggaggaaaag tgaaggtgct ggcgttggaa
     4441 ctttcctctt cgctggggag cgtggccttt ctcgacgaaa ccggcgccgg gcaagcgcag
     4501 atctttcccg ccgatcggaa ggattccggg tcgttctatg aaaacctgca agcgatatat
     4561 cggaactgcg gtgcgccgga ggtcatcgtg gtcgggctcg ggcccggatc ctacgccggg
     4621 acgcggattg cgatcgccac cgcgcttggt ctgcaggccg cctcgggcgc acgcttggtc
     4681 gggctgccgt ccgtctgcgc ttacgagccg gatgagtacg tggtcgtggg cgacgcccgg
     4741 cgcaattcct tctttttcgc ccatgtggtt cgcggtcgat gtgtggaggg gccgacgctg
     4801 gcgaccgagg aagaactgcg cgcgaagttg agctcatggc cgcatcagcc gatcttcgcc
     4861 gcccagccgt tgccgcaatt tgccaaaatc tcggtggcgc atccctcggc gcggcagttg
     4921 gctgagctgg cgctgcaagt gggattcgac cccgggcaaa cgctcgagcc aatttatttg
     4981 cgggagccgt atattacgat cgcaaagaaa tgaattcgtt aggtacggaa cgctgttggg
     5041 cggaggtgga ttgcgccgcg ctgcgtcaca acgcgggtgt cgtgcgggag cgagttggtc
     5101 ctcagccctc cctgctcgcg gtcgtcaaag cggacggcta cggccacggc gtggtcgagg
     5161 tggcccacgc cttgcgcgac gatgccgagc tcttcggggt tgcgaatctg cgcgaggcgc
     5221 tggtgttgcg tgagggcggc atcttgcacc cggttctcat cctcggcccg gcgttgccgg
     5281 aagagcgcgc ggcgatcaag gcccgcgggt tcatcgcctc ggtttcgagc ctggccgaag
     5341 cgctggcctt tgaggggaac gctgcgggaa ttaacttcgt catcgacacc gggatgggcc
     5401 ggatgggctg cacgccggag gacgcgctcg cggagctgga aaaaatcggc cgccacccgg
     5461 atctcatttt acacagcgtc tcgacgcatc taccggcggc ggatgaagac gtcgtcttca
     5521 cggaaaagga gttggaacag ttcgacgcgt tcgttaggca aatgcggttc cagattcccg
     5581 ggtcctacaa agtgcacgtt ctgctcagcg ccgggattct cggcttcacg caccatcgct
     5641 acgacatcgt gcgcgcggga ctgatgctct acggcagcgc gccgctgccg cacgaacaga
     5701 aagtcctgcg cccggcgttg accttgaagg cgcgcatcgc gctgctgcgc gacctgccgg
     5761 cgggctcgag cgtgagttac ggacgaactt ttattaccgc gcggccgacg cgggttgcca
     5821 cgatcagcgc cgggtatgcc gatggctacc cgcgctcgat ttcgaatcgc gacgggacgg
     5881 tcctgattgg cggaaggcgt tgtcccgtgc tgggccgggt cacgatggat ctcacaatgg
     5941 tcgatgtgac cgcgctaccg gaagtcaggg tgggcgatga ggtcgttttg atcggaaagc
     6001 agggcgccga agagattttg gccgcggagg tggcggagcg cgcggggacg atcgcctggg
     6061 aaattttcac tggcattggc agtcgagtgc ggcgggtgta tctctgatca gtgaattgag
     6121 attcgagaat cgtgaatcgg tgcacggtgc ggtctggcga ttcacgaatc tcgattcgag
     6181 attctcggac accatgttcc agatcatctt caatcaactg agtgcggccg agatggcggc
     6241 gttgccgaag accctccaac tcgagttgct cgcggaattt caaattctgc cggaagacct
     6301 cgaccacctc gacgcccggc gcttcggcgt gatcgagcgc gagggaaaaa aactgcatcg
     6361 ttaccgggca aaagactacc ggatttattt cgagcgcagt cccgaaggcg tgacggtgca
     6421 ccgcgtgctg cacaaaaaca cgctgcggga ttttctcttc cggagcaaac tgccggtggc
     6481 ggaagacgat cagctcggga agacgcgaga attctggaag ttgatcgagc aaggcgaacg
     6541 aacgcggaag acgtaggggc gcttttcgtt aggccgcagg gttgacagcc cggtcattgg
     6601 cctgagtaga cctgcgaaat cagcaaagcg attccgcttg tcgcgccccg cgctcgcgct
     6661 cccggaaaac taatggcgcg tccgtttctc ggaaggtagg acgctaaccg gatacgtttg
     6721 cggagcgagg cgtctacttg ggcag
//
LOCUS       DATMNS010000031         7905 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2183326, whole genome shotgun sequence.
ACCESSION   DATMNS010000031 DATMNS010000000
VERSION     DATMNS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7905
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183326"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VNW28_01210"
     CDS             <1..232
                     /locus_tag="VNW28_01210"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HXA08567.1"
                     /translation="PGTARQEAIAFSTGPGQAITYQIGKLQITKLLAEARLQQGENFN
                     LRAFHDFVWKNGNVPLSLQRWEYLGKDDDLPK"
     gene            complement(315..1106)
                     /locus_tag="VNW28_01215"
     CDS             complement(315..1106)
                     /locus_tag="VNW28_01215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXA08568.1"
                     /translation="MTITDHEFVDLATPTGPMRTHVLRPAAEGRYPGILLFSEIFQVT
                     APIRRTAALLAGHGFLVAVPEIYHEYEPAGTVLAYDDAGAERGNALKTTKPLDAYDTD
                     ARVVLKYLSEHPASTGKLGVMGICIGGHLAFRAAMNRGVLAAACFYATDIHKGGLGAA
                     MNDNTLDRIPEIAGEMLMVWGRQDPHVPLEGRLKIHAALEAAGTFFQWHEFNAAHAFL
                     RDEGPRYNPVLAQQSYALALEMFQRRLAEGDVIAAKPAGGTETKH"
     gene            complement(1125..1706)
                     /locus_tag="VNW28_01220"
     CDS             complement(1125..1706)
                     /locus_tag="VNW28_01220"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXA08569.1"
                     /translation="MRAARECGSRCFKMSIATKTGDAGETSLMYGRRVPKTDPRVDAY
                     GCVDELNSALGVVRATTGASLVAEQILSIQKELVTVMGELATAPEDLARYKKDGFNLT
                     TAAMVDRLTNAIDELEKDKTLYGKGWATPGNTPLAAALDMARTVCRRAERRVAALEGT
                     EHSVAEVLRYLNRLSDLCWLLARFAEKNGAAAA"
     gene            complement(1728..5849)
                     /gene="rpoC"
                     /locus_tag="VNW28_01225"
     CDS             complement(1728..5849)
                     /gene="rpoC"
                     /locus_tag="VNW28_01225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXA08570.1"
                     /translation="MNEHSWRELVGEERPVGFDQVAIGVASSDTMRSWSKGEVKNPET
                     INYRTFKPEKGGLFCERIFGPTRDWECSCGKYKRIKHKGVICDRCGVEVTLARVRRER
                     MGHIELAVPVSHIWFYKCMPSRLGLMLDMSARQLERVIYYEDYIVIDPGNTPLTKTQL
                     LTEVEYREAQEQYGDEFKAGMGADAVKVLLGEIELAKLNKELEEAMGNTKSKQIRKKL
                     AKRLKLVQGFASSHSRPEWMVLEVLPVIPPDLRPLVPLEGGRFATSDLNDLYRRVINR
                     NNRLKNLLQLKTPDVIIRNEKRMLQEAVDALFDNGRHGRAVTGAGNRPLKSLSDMLKG
                     KGGRFRQNLLGKRVDYSGRSVIVIGPELKLNQCGLPKKMALVLFEPFIIRRLKDLGYV
                     HTVRSAKKMIERQTPEVWDILEEVTKGHPVLLNRAPTLHRLSIQAFEPLLIEGEAIRI
                     HPLVCTAYNADFDGDQMAVHVPLSVEAQMEARMLMLAPNNIFSPSSGKPITTPSQDIT
                     LGCYYLTQNPRRAAGAAAETRLPLFDNATEVEFAMADGAVKTHDRIRLRNPDFGKETI
                     YGDAETRVIETTAGRVIFNEIWPDKLGFFNKAAGKKQLSDIIWRCYQVAGQQGTVETL
                     DRLKELGFAEATKAGVSIGISDMIIPKEKELQLANAYKQIKQVEQQYRKGIITDGERY
                     NKIIDIWTHAGDEISNVMFRTLEHNEGRKELNPVFLMVDSGARGNRQQVKQLAGMRGL
                     MAKPSGEIIERPITSNFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRKLVDAS
                     QDVIINEIDCETVNGITVRAIYEGDEEVVDLATRIIGRVSCETVVDPVTKKKIVKANQ
                     LIDEPIAAAIEKVGVESLKIRSVLTCETGRGVCAHCYGRNLATGQFVKLGEAVGIIAA
                     QSIGEPGTQLTMRTFHIGGTASQTFKQPIIKAKNDGTVQFNDLRTVQSLDGNWIALNK
                     NGSISLHNEEGREIERYNIVIGSVISMADGAKVKKGETFVQWDPYNVPILTDKSGKIE
                     FRDMIAGVTMKRDVDEATGLMGTVIIEHKEDLHPQIVIVGDDGEVAASYSIPAGAHVI
                     VEANHKIRAGALLAKTPRKIAKTKDITGGLPRVAELFEARRPKDAAEIAKIDGIVEIG
                     GTVRGKRKLILKDTATGAEEEHLIPLNKHMIVFKGDFVKKGSQLTEGPVVPHEILEIC
                     GPQDLQEHLVNEVQEVYRLQGVEINDKHIEIIVRQMLRKVKITDPGDTALLWGDQIDK
                     LEFEKQNAEVVKQGGKPAEATPVLLGITKASLETDSFISAASFQDTTRVLTEAATLGK
                     VDKLRGFKENVIMGHLIPAGTGFPAVREIKLVELGEQVGAPLMEEPEPQPALG"
     gene            complement(5883..>7905)
                     /gene="rpoB"
                     /locus_tag="VNW28_01230"
     CDS             complement(5883..>7905)
                     /gene="rpoB"
                     /locus_tag="VNW28_01230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXA08571.1"
                     /translation="KQLVSVAAGLIPFLEHDDANRALMGSNMQRQGVPLIVSESPLVG
                     TGLEGKVARDSRAVVVASEAGRVASVTADQIVVTKDGEMPESRKKLKTDLEAGVHVYE
                     LRKFMRSNAGTCVNQRPIVKKGQHVKRGQVIADGPNTENGELALGRNVLVAFMPWNGY
                     NFEDAIMISEKVVKEDIYTSIHIDEFEIGARDTKLGPEEITRDIPNVSEEALRNLGSD
                     GVIRVGAEVKPGDILVGKITPKSETELAPEERLLRAIFGEKAADVKDTSLTVPSGTYG
                     IVMDVKVSSRHEVAREKLTPAETKRQLKAITEDNRKKKDELTEQLTDALSNVLLGEKI
                     PLDVVNAQTGEIIIPANRKITKTLLRKLAMAYDHIEIDPSPIRNKIREIIAQYEHKFA
                     ELELDRERSMDRVESGDDIDPGIIKQVKVFVASKRKLSVGDKMAGRHGNKGVVARIVP
                     EEDMPFLADGTPVEIVLNPLGVPSRMNVGQVLETHLGVAAKALGFKVATPVFDGISEA
                     KIREYLNDAKKVDGFTWVQENGKARLFDGRTGDAFDQEVVVGYIYMLKLGHLVADKIH
                     ARAVGPYSLVTQQPLGGKAQYGGQRFGEMEVWALEAYGAAYTLQELLTVKSDDVQGRT
                     RIYESIVKGDNSLEAGTPESFNVLIKEMQSLGLDVKVGGQAPSFLENVA"
BASE COUNT         1370 a         2391 c         2387 g         1757 t
ORIGIN      
        1 tccggggaca gcccggcagg aagcgatcgc tttttccacc ggaccaggac aggccatcac
       61 ctaccagatc ggcaagctcc agatcaccaa attgctcgcc gaagcgcggc tccagcaggg
      121 cgaaaacttc aacctgcgcg ccttccacga tttcgtgtgg aagaacggca acgtgccgct
      181 ttcgcttcaa cgctgggagt atctcggcaa ggacgacgac ctgccgaagt agttgcgcgg
      241 cgcgagaaac gttgggtagc gcacgcagaa tgcgtgcgcc acccggaaca gggttgcgaa
      301 accaaacgcc agcctcagtg cttcgtctct gtgccgcccg ccggtttcgc ggcaatcaca
      361 tcgccctccg cgagccggcg ttggaacatt tccagcgcga gcgcataact ctgctgagca
      421 agcacaggat tgtaacgcgg accttcgtca cgcagaaaag cgtgggcggc gttgaactcg
      481 tgccattgaa aaaaggtgcc cgccgcttcg agcgcggcat ggattttcaa gcggccttcc
      541 agcgggacgt gcggatcctg gcgaccccag accatcagca tctcgccggc gatctcggga
      601 atacggtcga gcgtgttgtc gttcatggcc gcgccgaggc cgcccttgtg aatgtcggtg
      661 gcgtaaaagc aggcggcggc gaggacgccg cgattcatcg ccgcgcggaa ggcgagatga
      721 ccgccgatgc agatgcccat gacgccaagc ttccccgtcg aggcgggatg ttcgctcaaa
      781 tatttcaaga ccacccgcgc gtcggtatcg taggcgtcga gcggcttggt tgtcttgaga
      841 gcattgccgc gctccgctcc cgcatcgtcg taggcgagca cggtgccggc aggttcgtac
      901 tcgtggtaaa tctccggtac ggcgacgagg aacccgtgcc cggcgagcaa cgccgccgtc
      961 cgacgaatgg gggcggtgac ctgaaagatt tccgagaaaa gcaggatgcc cggatagcgg
     1021 ccttccgccg ccgggcgcaa gacgtgcgtg cgcatcgggc cggtgggcgt cgcaagatcg
     1081 acgaattcgt gatcggtaat ggtcatggcg ggttcaagaa aacctcacgc cgcagcggcg
     1141 ccgtttttct ccgcgaagcg cgcgaggagc cagcagagat cagagagccg gttcaggtaa
     1201 cgcaggactt ctgcgacgga gtgctctgtg ccctcgaggg ccgcgacacg gcgctcggcg
     1261 cggcgacaaa ccgttcgcgc catgtcgagc gccgcagcga ggggcgtgtt gccgggagtg
     1321 gcccagcctt tgccgtagag cgtcttgtct ttctccagtt cgtcaatcgc attcgttagg
     1381 cgatcgacca ttgcggccgt cgtgaggtta aagccgtcct ttttgtaacg cgccaggtcc
     1441 tccggcgccg tcgccagctc gcccatcacg gtcacgagct ctttttggat ggagagaatt
     1501 tgttccgcga ctaacgacgc gccggtcgtt gcgcggacca cgccgagcgc ggaattcagt
     1561 tcgtcgacac agccgtaggc gtcgacgcgc gggtcggtct tgggcacacg ccggccatac
     1621 atgagcgagg tttcgccggc gtcaccggtt ttggttgcga tcgacatctt gaaacaacga
     1681 gagccgcact cgcgtgcggc tctcatttct acagcatgga acttatttta gccaagcgcg
     1741 ggttgcggtt ccggctcttc catgagcggg gcgccgacct gctcgccgag ctcgacgagc
     1801 ttgatctcac gcaccgccgg gaagccggtg ccggccggga tgaggtgacc catgatcacg
     1861 ttctccttga agccgcgcag cttgtcgact ttgcccagag tggctgcctc ggtcaggacg
     1921 cgagtcgtgt cctggaacga agccgccgaa atgaagctgt cggtttcgag cgacgccttg
     1981 gtgatgccaa gcagcaccgg ggtcgcttcg gccggcttgc cgccctgctt cacgacttcg
     2041 gcgttttgtt tttcgaactc gagtttatcg atctggtcgc cccagaggag cgcggtatcg
     2101 cccggatcgg tgatcttcac cttgcggagc atttgcctaa cgataatctc gatgtgcttg
     2161 tcgttaatct cgacgccttg gagccggtag acttcctgca cttcattcac gaggtgctcc
     2221 tgcaaatcct gcggtccgca aatctccaga atctcgtgcg gcacgacagg cccttcggtt
     2281 agttgcgagc ctttcttcac aaagtcgcct ttgaacacga tcatgtgctt gttcagcgga
     2341 atgaggtgct cttcttctgc gccggtcgcg gtgtccttca gaatcaactt ccgtttcccg
     2401 cggaccgtgc ccccgatttc gacgatgccg tcgatcttcg cgatctcggc cgcatctttc
     2461 ggacggcgcg cctcgaacaa ttccgccacc cgcggcagac cgccggtgat gtccttcgtt
     2521 ttggcaatct tgcgcggggt cttggcgagc aatgcgccgg cgcgaatctt gtggttcgct
     2581 tcgacgatga cgtgcgcgcc cgccggaatg gaataactcg cagccacttc gccgtcgtcg
     2641 cccacaatca cgatctgcgg atgcaggtct tccttgtgtt cgataatgac ggtgcccatc
     2701 agcccggtcg cctcgtccac gtcgcgtttc atggtgacgc ccgcgatcat gtcgcggaat
     2761 tcgatcttgc cgctcttgtc ggtcaagatc ggaacgttat agggatccca ctgcacgaac
     2821 gtttcgccct tcttcacttt ggcgccgtcg gccatcgaga tgacggaacc gatcacgata
     2881 ttgtagcgtt cgatttcccg gccctcttcg ttatgcaggc tgatcgagcc gttcttatta
     2941 agcgcgatcc agttgccgtc gagtgactgc acggtgcgca gatcgttgaa ctgaaccgtg
     3001 ccgtcattct tcgccttgat gatcggctgc ttgaaggtct ggctcgcggt accgccgatg
     3061 tggaacgtcc gcatcgtcaa ctgcgtgccc ggttcgccga tcgattgcgc ggcgataatg
     3121 ccgactgctt cgcccagctt gacgaactgg ccggtcgcca ggttgcgtcc gtagcaatgc
     3181 gcgcaaacgc cgcgtccggt ttcgcaggtc aggacggagc gaatcttgag gctttcgact
     3241 ccgacttttt caatcgcggc ggcgatcggc tcgtcgatta actgattcgc cttgacgatc
     3301 tttttcttcg tcaccggatc gacgaccgtt tcgcagctca cgcggccgat gatgcgggtc
     3361 gcgagatcga ccacttcttc gtcgccttca taaatggcgc gcaccgtgat gccgttgacc
     3421 gtttcgcaat cgatctcgtt gatgatcacg tcctgcgatg cgtcgaccag cttcctcgtc
     3481 aggtaaccgg agtccgcggt cttgagcgcg gtgtccgcca gacctttgcg cgcgccgtgc
     3541 gtggagatga aatactcaag cacagtcaga ccttcgcgga agttcgaggt gatcggccgc
     3601 tcgatgattt cgccggaggg cttcgccatc aaaccgcgca tgccggcaag ctgcttcacc
     3661 tgctgccggt tgccgcgcgc gccggagtcg accatcagga agaccgggtt caattccttg
     3721 cggccctcgt tatgctcgag ggtgcggaac atgacgttgg aaatctcgtc gcccgcgtgc
     3781 gtccagatgt cgatgatctt gttgtagcgc tcgccgtcgg tgatgatgcc tttgcgatac
     3841 tgctgctcga cctgcttgat ctgtttgtag gcattcgcga gctgcaactc tttctccttc
     3901 ggaataatca tgtccgaaat gccgatcgac acgcctgcct tggtcgcttc cgcgaagccc
     3961 agttccttga ggcgatcgag cgtctcgacg gtgccctgct gcccggcgac ctgatagcaa
     4021 cgccagatga tgtcgctgag ttgcttcttg ccggcggcct tgttgaagaa accgagctta
     4081 tcgggccaga tctcgttgaa gatgacacga cccgcggtcg tttcgatgac ccgcgtttcg
     4141 gcgtcgccgt agatcgtttc cttgccgaaa tccggattcc gcaggcgaat ccgatcatgc
     4201 gttttgaccg ccccatcggc catcgcgaac tcgacttcgg tcgcgttgtc gaagagcggc
     4261 aggcgcgttt ccgccgcggc gcccgcggcc cgccgcggat tttgcgtaag gtaatagcag
     4321 cctaacgtaa tgtcctgcga cggagtggta atcggtttcc cactcgaagg cgaaaagatg
     4381 ttgttcggcg caagcatcag catgcgcgct tccatttgcg cctcgaccga aaggggcaca
     4441 tggaccgcca tctggtcgcc gtcgaagtcc gcgttgtagg cggtgcagac gagagggtga
     4501 atgcggatcg cctcaccttc gatcaggagc ggttcgaaag cctggatcga aagacgatgc
     4561 aaagtcggcg cgcgattcag gaggaccggg tggcccttgg tcacttcctc gaggatgtcc
     4621 cagacttccg gcgtttgacg ctcgatcatt ttcttcgcgc tgcgcaccgt gtggacgtag
     4681 ccgagatcct tcaggcgacg gatgatgaac ggctcaaaga ggacgagcgc cattttcttc
     4741 ggcaacccgc attggttcag tttcaactcg ggaccgatga cgatgaccga gcggcccgag
     4801 taatcgactc gtttgcctaa caagttctga cggaaacgtc cgcccttgcc cttgagcatg
     4861 tcgctgagcg atttgagcgg ccgattgccc gcgccggtga ccgcgcggcc gtgacgaccg
     4921 ttgtcgaaga gggcgtcgac tgcttcctgc aacatgcgtt tttcgttgcg gatgatgacg
     4981 tccggcgtct tgagctgaag gaggtttttg agccggttgt tgcggttgat cacgcgacga
     5041 tagaggtcgt tcaaatccga agtcgcaaac cggccgccct cgagcggcac gagcggacgc
     5101 agatcgggcg ggataaccgg gagcacttcg agcaccatcc attcgggacg ggaatgggag
     5161 gaggcgaagc cctgcaccag cttcaaacgt ttcgcgagtt tcttgcgaat ctgtttgctc
     5221 ttggtgttgc ccatcgcttc ctcgagctcc ttgttcagct tggcgagttc gatctcgccg
     5281 agtagcacct tgaccgcgtc ggcgcccatg ccggccttga attcatcgcc gtattgttcc
     5341 tgcgcttcgc ggtattccac ctcggtcagg agctgcgttt tggtgagggg cgtattgccc
     5401 ggatcgatca cgatgtagtc ctcgtaatag ataacgcgct cgagctggcg cgcgctcatg
     5461 tcgagcatca ggccgaggcg cgacggcatg cacttgtaaa accagatgtg cgagacaggg
     5521 accgcgagct cgatgtggcc catgcgttcc cggcgcaccc gcgcgagcgt cacctcgacc
     5581 ccgcaacgat cgcagatcac gcccttgtgc ttgatccgct tgtattttcc acaggagcat
     5641 tcccagtcgc gcgtcggacc gaaaatgcgt tcgcagaaca agccgccttt ttccggtttg
     5701 aaggtgcggt agttaatcgt ctccggattc ttgacttcgc ccttgctcca ggaacgcatc
     5761 gtgtcgctcg aagcgacccc gatggcgact tgatcgaaac ccacggggcg ttcttcgccc
     5821 accaattccc gccaggaatg ttcgttcata atgagagctg tgtttctaag tttggtagat
     5881 tgttaggcaa cgttctcgag aaagctgggc gcctggccac cgactttgac gtccaggcct
     5941 aacgactgca tttccttgat taacacgttg aaagattccg gcgtgccggc ctcgagcgag
     6001 ttgtcgccct tcacgatcga ctcgtagatc cgggtgcgtc cctgcacgtc gtccgacttg
     6061 acggtgagga gttcctgcag ggtgtaggcc gcgccgtaag cctcgagggc ccacacttcc
     6121 atctcgccga agcgctgacc gccgtactgc gctttgccgc cgagcggttg ctgggtgacg
     6181 agcgagtaag ggccaaccgc gcgggcgtgg attttgtccg ccaccaaatg gccgagcttg
     6241 agcatgtaga tgtagccgac cacgacttcc tggtcgaacg cgtcgccggt gcgaccgtcg
     6301 aagaggcgcg ccttgccgtt ctcctggacc caggtaaagc catcgacttt tttggcgtcg
     6361 ttcaggtact cgcggatttt cgcctccgaa atgccgtcga aaaccggggt cgcgaccttg
     6421 aagcccagcg ctttggccgc gacgccgagg tgcgtttcga gcacctgacc gacgttcatg
     6481 cggctcggca cccctaacgg attgaggacg atctcgaccg gagtgccatc agcgaggaac
     6541 ggcatgtcct cttcgggcac gatgcgcgca acgacaccct tgttaccgtg gcgaccggcc
     6601 atcttatcgc cgacgctcag cttgcgcttg ctcgcgacga acactttgac ctgtttgatg
     6661 atgccgggat cgatgtcgtc accgctctcg acgcgatcca tggagcgttc gcgatcgagc
     6721 tcgagctccg cgaacttatg ctcgtactgg gcgatgattt cgcggatctt gttgcggatc
     6781 gggctcgggt cgatctcgat gtgatcgtag gccatcgcca gtttgcgaag cagtgtcttg
     6841 gtgattttgc ggttcgccgg gatgatgatt tcgccggtct gcgcattgac gacgtcgagc
     6901 ggaattttct cgccgaggag cacattggag agcgcgtcgg tcagttgctc cgtcaattcg
     6961 tctttctttt tccggttgtc ttccgtgatc gccttgagct ggcgtttggt ttccgccggt
     7021 gtcagttttt cccgcgcaac ttcgtggcgt gaagaaacct tcacgtccat cacgatgcca
     7081 taggtgcccg acggcacggt cagcgacgtg tctttcacgt cggccgcttt ttcgccgaaa
     7141 atggcgcgga gcaggcgctc ttcgggagcc agttccgtct cgcttttcgg ggtgattttg
     7201 ccgaccaaaa tgtcgcccgg cttgacttcc gcaccgacgc gaatgacccc gtcggaaccg
     7261 aggttgcgga gcgcttcttc gctgacgttc ggaatgtccc gcgtgatttc ttcgggcccg
     7321 agtttggtat cgcgcgcgcc gatttcgaac tcgtcaatgt ggatggaagt gtagatgtct
     7381 tccttcacca ccttctcgga aatcatgatc gcgtcctcga agttgtagcc gttccacggc
     7441 atgaacgcga cgagcacatt gcggccgagc gccagctcgc cgttctcggt gttcgggccg
     7501 tcggcgatga cttgtccgcg tttcacgtgc tgccctttct tgacgatcgg tcgctggttc
     7561 acacaggtgc cggcgttcga acgcatgaat ttgcgcagct cgtaaacgtg cactcccgct
     7621 tccagatcgg tcttcagttt tttgcgactt tccggcattt cgccgtcttt cgtgaccacg
     7681 atctggtcgg ccgtgactga cgcgacgcgg cccgcttcac tcgcgaccac caccgcgcgg
     7741 gaatcgcgcg ccacttttcc ttcgagaccg gtgccgacga gcggcgattc ggagacgatc
     7801 agcggcacgc cctggcgttg catgttcgag cccatgagcg cgcggttggc gtcatcgtgc
     7861 tcgaggaagg gaatcaagcc cgcggcgacc gagaccaact gtttc
//
LOCUS       DATMNS010000032         3222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1475003, whole genome shotgun sequence.
ACCESSION   DATMNS010000032 DATMNS010000000
VERSION     DATMNS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475003"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..2034)
                     /locus_tag="VNW28_01235"
     CDS             complement(148..2034)
                     /locus_tag="VNW28_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HXA08572.1"
                     /translation="MDAELQKLVDAGKLTTAGAAQLERLKPGAFCLHKSWGFGRVADW
                     NLLLNQIVIDFGKKKAHPMQLPYAGENLTPIPAEHFLAQKASDLAGLKQRLKENPTGA
                     MRNILESLGGKATQAQISGWLVGDVFTEAEFKRWWDSTKKLLKKEGHFLIPTKKNDPI
                     ELRSAPVSRADELLTFFNQARQPKEQTAALDQVIKLRDEFADPEKQLQPLIDAIENTA
                     RRNQRLNPAMAFEFVIARDDLRQHEPKLRSSIPDLTLERLIVEEEPRLAAILPKLPSA
                     KERRVLHGLPAALGERWTTRALQLMQGNNARLVASMAKVFAETGKEAELRSALDRSLR
                     EHSATSEMLYWLCKSRAEWPELINPELLSAILSACERDQHNESNSRSTRLRDLLLSDR
                     ALIPDIFAGADLSLARDAMRRLMLSPVFDELTKRSLMARIIKLYPDLQSLVSGEQTEG
                     KSEALVVSWSSLQKRKAELEELVNKKIPENSKEIGVARSYGDLRENFEFKAAKEMQAV
                     LMRRKSELERALHLARGTAFENPDTSQVSIGTVVTLRETTSGREETYTILGAWDGDPE
                     RAIISYQTAIGQSLLGRRLEEVVELNAEEDTGRYAIVAIEPAPVDEVPADPELLEPVA
                     SEMA"
     gene            2140..3069
                     /locus_tag="VNW28_01240"
     CDS             2140..3069
                     /locus_tag="VNW28_01240"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HXA08573.1"
                     /translation="MPVNIWFWVAFHLGVLAALAVDLLSFHGPHTIDLPSAVRRSLVW
                     IALSLGFNVIVWSWQGADQGIDFFTGYLIEYSLSVDNIFVFVLIFAYFRVPPQYEHRV
                     LIWGILGAIVMRGLMIWIGVELVARFHFVLYLFGAFLLITGLRMLRRQTQQLDLDNNF
                     VLKSARRLLPVTNDYHGPKFIALVGGRRMLTPLALVLIVIDVMDLVFAVDSIPAVLAI
                     TQDTFIIYTSNICAILGLRSLYFLLANLVTRFVYLRFGLAMILCLVGSKMIAARWIRM
                     PNWLSLLLIAAVLLITILASLRASKPRPAPPKR"
BASE COUNT          508 a         1027 c          958 g          729 t
ORIGIN      
        1 acatgctgag cgcgctcggc agcacgggcc gcctcggcct gaccgcggtc ggcaattcgc
       61 ccgaggaagc gcgcgtcatt ttcgaccagg ccaaaagcgc gctggatgag gaagccagtt
      121 tcgacgagct ctagccgccg ctcatcgtca ggccatttcg gaagcgaccg gctcgagcag
      181 ctcgggatcg gccgggactt catcgaccgg ggccggttcg atcgcgacga tcgcgtaacg
      241 cccggtatcc tcttccgcgt tgagttccac gacctcttcg aggcgccggc ctaacaatga
      301 ctgcccaatc gctgtctggt aggaaatgat ggcgcgttcc ggatcgccat cccacgcgcc
      361 aaggatggtg taagtctctt cgcggccact cgtggtttca cggagggtca ccaccgtgcc
      421 gatggagacc tggctcgtgt cgggattctc gaaagcggtt ccacgcgcga ggtgcagcgc
      481 gcgctccagc tcggacttgc gtcgcatgag gacggcttgc atttccttcg cggctttgaa
      541 ttcgaaattc tcgcgcaagt cgccgtagga acgggccacc ccgatctcct tgctgttctc
      601 cgggattttc ttgttcacca gctcttcgag ctcggctttg cgtttctgca ggctgctcca
      661 ggaaacgacc agcgcttcgc tcttcccctc cgtttgctcg ccgctgacga ggctttgcag
      721 gtccgggtaa agcttgatga tccgcgccat gagagagcgc ttggtcaact catcgaagac
      781 cgggctgagc atgagacggc gcatggcatc gcgcgcgagg ctgaggtccg cgccggcaaa
      841 gatgtcgggg atcaacgcgc ggtcggaaag gagaagatcg cgcagccgcg tgctgcggct
      901 gttgctctcg ttatgctgat cgcgctcgca ggccgaaagg atggcgctga gcaattcggg
      961 gttgatcagc tccggccatt cggcgcggct tttgcaaagc cagtaaagca tttcgctggt
     1021 cgcggaatgt tcccgcaggc tccggtcaag ggcgctgcgc aattccgctt ccttcccagt
     1081 ttcggcgaag actttggcca tcgaggccac gaggcgggcg ttatttccct gcatgagttg
     1141 cagggcgcgc gtggtccagc gctcgcccag ggcggcgggc aggccgtgca ggacgcggcg
     1201 ttctttggcg gaagggagtt tcggcaggat ggcggcgagg cgcggctctt cttcgacgat
     1261 caatcgctcg agcgtcaggt cgggaatcga gctgcgcaat ttcggctcgt gctggcgcaa
     1321 atcgtcgcgg gcgatgacaa actcgaaagc catcgcggga ttgaggcgct ggttccgtcg
     1381 cgcggtgttc tcgatcgcgt cgatcagggg ctgcagttgt ttttccggat ccgcgaattc
     1441 gtcgcgcagc ttgatcacct gatcgagcgc agctgtctgt tccttcggct ggcgcgcctg
     1501 attgaaaaag gtgagcaact catcggcgcg cgagaccggg gcggagcgca gctcgatcgg
     1561 atcgttcttc ttcgtcggga taagaaagtg gccttccttc ttgagcagct tcttggtcga
     1621 gtcccaccag cgcttgaatt ccgcctcagt aaaaacgtcg cccaccaacc agccactgat
     1681 ctgggcctgg gtcgcttttc cgcccaggct ctcgagaatg ttccgcatcg cgccggtcgg
     1741 attttccttg agccgctgct tgaggccggc caggtcgctc gccttttgcg cgaggaaatg
     1801 ttccgccgga atcggcgtca ggttttcgcc cgcgtacggc agctgcatcg ggtgcgcctt
     1861 cttctttccg aagtcgatca cgatctgatt gaggagcaga ttccaatcag cgacgcggcc
     1921 gaatccccag ctcttatgga gacagaaggc gccgggtttg agtcgttcga gttgggccgc
     1981 acctgcagtt gttagcttcc cggcgtccac caacttttgc agttctgcgt ccataagatg
     2041 ttcacgttta cgatgcacga gccgttgctc ccgcgcaaac gacgccgccc gctgtttcgc
     2101 ggttgctcgc ccacttcacc cgtggaagcc tagcgccgcg tgccggtcaa catctggttt
     2161 tgggtcgcct tccatctcgg tgttctcgcc gcgctcgcgg tcgacctgct gagtttccac
     2221 ggaccgcata cgatcgacct gcccagcgcc gtccgccgga gcctcgtctg gatcgcgctc
     2281 tcgctcggct tcaacgtgat cgtctggtcc tggcagggcg ccgatcaggg gatcgatttc
     2341 ttcaccggtt atctgatcga gtactcgttg agcgtcgata acatctttgt cttcgtcctc
     2401 atcttcgcct atttccgcgt gccgccgcag tatgagcatc gcgtcctcat ttggggaatc
     2461 ctcggcgcga tcgtgatgcg gggcctgatg atctggatcg gcgtggagct cgtcgcgcgc
     2521 tttcatttcg tcctctacct cttcggcgcc tttctcctca tcaccggtct gcgcatgctc
     2581 cgccggcaaa cccagcaact cgatctcgac aacaatttcg tcctgaagag cgcgcgccgg
     2641 ttgctcccgg tgacgaacga ttatcacggc ccgaaattca tcgcgctcgt tggcggccgg
     2701 cgcatgctca cgccgctcgc gctcgtcctc atcgtgatcg acgtgatgga cctcgtcttc
     2761 gccgtcgact cgatccccgc cgtcctcgcg atcacgcagg acaccttcat tatctacacc
     2821 tccaatattt gtgcgatcct cggcctgcgc tcgctctatt tcctgctcgc gaatctggtc
     2881 acgcgctttg tttacctccg cttcggcttg gcgatgatcc tttgtcttgt cggctcgaag
     2941 atgatcgcgg cccgctggat ccgcatgccg aactggcttt cgcttctcct gatcgcggcc
     3001 gtccttctca tcaccattct cgcctcgtta cgagccagca aaccccggcc ggctccgccg
     3061 aagcgataaa acaagggttg ccatcgttta ccggaatcgg tttactcgcg ccgcatcatg
     3121 catcgcctcc tcgtgacgct ttgcctcgcc ggttttgcga ccagtgcatg ggccgatatc
     3181 caggatccgc ccggagcgga tcacggcccg acgcgcaagc tc
//
LOCUS       DATMNS010000033         6885 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1476326, whole genome shotgun sequence.
ACCESSION   DATMNS010000033 DATMNS010000000
VERSION     DATMNS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6885
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1476326"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VNW28_01245"
     CDS             complement(<1..783)
                     /locus_tag="VNW28_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXA08574.1"
                     /translation="MSSFKDRSQDPNSICSRSQKSRLTLRGGDMVSSICQRLFLITAC
                     AACVILLLAGCSRKPAQTAPTAPEVLVTTVTPRDVPRVLERVATLDGFINANINAQVQ
                     GYIVSRAYQEGSVVKKGDVLFQIDPRPFEAGLAQAKGTLAKDNANQVKSDADEKRALD
                     LFNKKVISDQERDTAIAAAGSGRANVEADQAAVDQAELNLGYAKVTAPIDGVAGFANN
                     QVGDLVGPNTGPLTTVSQVDPIKAIVTVGEAPWTDFISRHPDA"
     gene            complement(1039..2085)
                     /locus_tag="VNW28_01250"
     CDS             complement(1039..2085)
                     /locus_tag="VNW28_01250"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXA08575.1"
                     /translation="MKKQIILTTALAALLPLAAFAQSPAPPVSQPAQQPIPPVPPVPP
                     GPPPMNYGRMHDHDMGPKEPVTFLGVETSNVPRVLSEQMGLPRGFGVVVDYVVPESAA
                     AAAGVQQSDIIRMLNDQIILEPSQLGKLVRSFADGATVNLTLLRKGKEVKVTVTLKKH
                     DESKGNGPFGYERDWKWDDLGKMNFDFQMPDMTAVREAVARAKQEALRAGDEARRAAR
                     NLRVVTTDDDTTKTTRVDLGKATITFSDAEGELKMEKVDGRKMLTAKDALGKVLFNGP
                     IDTPEQRAKIPAGVRERLEKLENQDMPEIPPTPEAPRAPDPDESARLQNRRMQRAGLS
                     PNNHTGWVRSTVLL"
     gene            complement(2104..2607)
                     /locus_tag="VNW28_01255"
     CDS             complement(2104..2607)
                     /locus_tag="VNW28_01255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08576.1"
                     /translation="MNDFSELESELKKLRPAPVSDELFARLECALAQPDSAPTAGFLP
                     RPAQRRNIWWSLGLGLAASSALVLIGLAFFAEPPRTPPVVAQQDTPAPSGPVASGFQP
                     SALTQVVYRQRDEGLVFSPNQSERPLRRVRYETRETMQWRNPQTGASLRVSYPAEQIV
                     LTPVPFQ"
     gene            complement(2600..3094)
                     /locus_tag="VNW28_01260"
     CDS             complement(2600..3094)
                     /locus_tag="VNW28_01260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXA08577.1"
                     /translation="MESTLTDEDWKTCFAQLGPALLLFARRWTNSRADAEDIVQDAFV
                     RFWRRQHSPENRALLYATVRSTALDRLRSDQRRARREATVALDGATHWEPQFTTLDEG
                     QQLLAAAVERLPDEQQEVVVLKIWNDLTFAEIAQILAISPNTAASRYRYALGALRKAM
                     QPYE"
     gene            3133..3987
                     /locus_tag="VNW28_01265"
     CDS             3133..3987
                     /locus_tag="VNW28_01265"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXA08578.1"
                     /translation="MQFLSSRVKLPAALLSLCAAATSLAEVPATDGNARSLAAAESAF
                     AQEALARGTRRAFLDALSADGVVFQPGPQNGRKVWEARKDSGGILAWQPVLAVVAASG
                     DFGYTTGPWTFKKTAADEQPAAFGQFVSIWRREAGKWKLLFDLGTENPAPTTPAPALR
                     LRDNRLPNESAAAARATLLEQDRSYAADRLKNFSALAADDVRLYLPAKVPVVGKTGAV
                     AALQEAAGTTTLREPKAEVASSGDLGFAWGEYAAGNAADATGYYLHIWCKNEQGQWRL
                     ALDLLSPR"
     gene            4002..4748
                     /locus_tag="VNW28_01270"
     CDS             4002..4748
                     /locus_tag="VNW28_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HXA08579.1"
                     /translation="MQPASLMPSVSIIVPTLNEAENVEPLTRQIMASGIHPLEIIFVD
                     DGSTDGTGDRVRELAAEFPVRLVVRENPALGLSGAVVAGAQDATGDLLVVMDADLSHP
                     PSRIPDLVRPLQAGACEMVVGSRYVRGGTTPGWPLWRKIMSRAASAFAYPLTRVHDSM
                     CGFFAIPRPLLLERAPAATGFKIAFEIIVHGERRLRVQEIPIAFRDRARGASKMTFRV
                     AAVFFVRWLAAVVRRKPARAAAAAARAPVR"
     gene            complement(4693..5958)
                     /locus_tag="VNW28_01275"
     CDS             complement(4693..5958)
                     /locus_tag="VNW28_01275"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HXA08580.1"
                     /translation="MARFLDWLNSRTGFRLLVVLFILLTIVFSIVPLVNLAAGRGAKD
                     YKLWFSTGQTVLHGGAIYPPQGQRFPFMYPPTAAEMLAPLSLLGQAGLIAALVLVNSL
                     AWWACIVWSLRLANPGGERPHAMLYLVPNLVIMVYVWSCYLLGQPTLVLLALLLAGFL
                     ALRAGRSWSAGTMFALAAAIKAFPVAAIAYLIYRRYWIATLSMLLSLALLLLVLPAGF
                     RGWAGARQDLTRWTDGMLFQYDEKGIAQRPERSVAWKNQSLAGVANRFLRKVDVDATR
                     PPDKPIFVNFIDLPFKVVNAIIVALSLLLGLVYLGAMPPGAGRTWETDAIEWSMLLLM
                     LLIAAPLSFNYLFAFLLLPFAIITRVWLVRPGSPLRWWALAAVLLLALTIPLQHWSQS
                     YGNTLFAALLLFIGLALELRRLRRAPVSA"
     gene            complement(5962..>6885)
                     /locus_tag="VNW28_01280"
     CDS             complement(5962..>6885)
                     /locus_tag="VNW28_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HXA08581.1"
                     /translation="LETIEDFEQMVGKHQAIIASSSYWGEQSFPTANLQMIWRHGSLP
                     LVFWSPWDRPYDQKKGPDRFGLNAILAGTWNRYIDDWADAARAFGHPLLVSFGDEMNG
                     DWFPWSGIYYGGGKKIPGRPNEWEGPDLFKRVFRYVVDRVRARGAHNIQWIFQTNNYS
                     FPMDTWNLAAAYYPGPDYVDWLGLSVYGQQFDDEPWSNFLPLLDWPYREIAALDPKKP
                     IMITEWAAGEFPNSGNKAQWMRDAFEVMRTKCPRVKAEIYWHERWQNDDQSYSNLHIN
                     SSPEALAAYRKAVADPYWLGDLMLRPAARTK"
BASE COUNT         1288 a         2115 c         2210 g         1272 t
ORIGIN      
        1 agcatcagga tggcgcgaaa taaaatcggt ccagggcgct tcgcccaccg tgacgatggc
       61 tttgatcgga tcaacttgtg aaaccgttgt caggggtccg gtgttcgggc cgacgagatc
      121 gccgacctga ttattcgcga agcctgcgac accgtcgatt ggagccgtta cttttgcgta
      181 tccaagattc agctccgcct ggtccaccgc ggcttggtcg gcttcgacgt ttgctctgcc
      241 ggaaccggcg gccgcaatgg ctgtgtctcg ctcctggtcg ctgattacct ttttgttgaa
      301 caagtcgagc gcgcgttttt catccgcatc tgatttcacc tggttggcat tgtctttcgc
      361 gagagtgcct ttcgcctgcg ccaatccggc ctcaaacgga cgcgggtcga tctgaaaaag
      421 gacgtcgccc ttcttcacca cgctgccctc ctggtaggcg cgcgaaacga tgtagccttg
      481 cacctgcgcg ttgatgttgg cgttgatgaa accatccagt gtcgcgacgc gttcgagcac
      541 gcgcggcaca tctcgcggcg tcaccgttgt caccagaact tcgggcgcgg tcggcgccgt
      601 ttgcgccggt ttgcgcgaac aacctgccag caaaaggatc acgcatgcag cgcaggctgt
      661 gatcaggaac aatcgctgac agatcgacga aaccatatct ccgcccctca gcgtaagccg
      721 acttttttgt gaacgcgagc agatcgaatt ggggtcctgg gatcgatcct tgaagctgga
      781 catactcatt cgagtgattg aagcattcgg cctcttcgct gccgacaatt ttctgcgaaa
      841 tcggatcggt cctggcccgc ggacgttcaa gggcgcccga tcgtctgacg cacaaatgaa
      901 gggcagcgtt actcaccggc gcttcgcata gcgaagcggc tacagcgtgg tggctacagg
      961 agcgaggcca cggaatggcg gctacagacc gcgctgcgca cagcgaagcg gctacaactg
     1021 ctacagaagc gaagcgtttt acagcagcac cgtgctgcgg acccagccgg tgtgattgtt
     1081 aggcgagagc ccggctcgtt gcatccgccg gttttgcagg cgagccgatt catccgggtc
     1141 gggcgcgcgg ggcgcttccg gcgtcggcgg aatttcgggc atgtcctggt tttccagctt
     1201 ttccaggcgt tcccgcacgc cggccgggat tttggcgcgc tgctccgggg tatcgatcgg
     1261 tccattgaag agcactttcc ccagcgcgtc tttggcggtc agcattttcc ggccatcgac
     1321 tttctccatc ttcagctcgc cctccgcatc ggagaaagtg atcgtcgctt tcccgagatc
     1381 gacccgggtg gtcttggtcg tgtcgtcgtc ggtcgtcacc acgcgcaaat ttcgcgccgc
     1441 ccggcgcgct tcgtcgcccg cccgcagcgc ttcctgtttc gcccgcgcga ctgcttcccg
     1501 gaccgccgtc atatccggca tttggaaatc aaaattcatc tttcccaagt cgtcccattt
     1561 ccagtctcgc tcgtagccga acggtccgtt acccttggac tcgtcgtgct tcttcagcgt
     1621 cactgtgact ttcacttcct tgcctttgcg caggagtgtg aggttgaccg tggcgccgtc
     1681 ggcgaagctg cgcaccagtt tcccgagctg gctgggttcg aggatgatct ggtcgttcag
     1741 catccggatg atatcgctct gctgcacgcc ggccgcggcc gcggcgctct ccggcacgac
     1801 gtaatcgacc accacaccga agccgcgggg caggcccatc tgttccgaca acacccgcgg
     1861 aacattggag gtctcgacgc cgagaaaggt gaccggttcc ttcggcccca tgtcgtggtc
     1921 atgcatgcgt ccgtaattca tgggaggcgg cccgggcggg acgggaggca ccggcgggat
     1981 gggctgctgc gccggttgcg acacgggcgg agcgggtgat tgcgcgaagg cggcgagcgg
     2041 gagcaacgcg gccagcgcgg tcgtgaggat gatttgtttt ttcatggatt tagatgctgc
     2101 gactcattga aagggaaccg gcgtgaggac gatctgctcc gccggatagg agacacgcag
     2161 ggaggcgccg gtctgcggat tgcgccactg catcgtctcg cgggtttcgt aacggacccg
     2221 ccggagcggg cgctccgatt ggttaggcga gaaaaccaga ccctcgtcgc gctggcggta
     2281 gacgacctgc gtcagcgccg agggttgaaa gccgctggcc accggccccg agggagccgg
     2341 ggtgtcttgt tgtgccacga ccggtggcgt ccgcggcggc tcggcgaaaa aggccagccc
     2401 gatgagaacg agtgcggaag aggccgcgag cccaagacca agcgaccacc agatatttcg
     2461 ccgctgcgcc gggcgcggaa gaaaaccagc ggtgggcgcc gagtccggtt gcgccagggc
     2521 gcattccagc cgcgcgaaca gctcgtccga caccggcgcg ggccgcaact ttttcagctc
     2581 cgattccaac tcggaaaaat cattcatagg gctgcatcgc tttcctgagg gcgccgagcg
     2641 cgtagcggta gcgcgaggcg gcggtgttcg gagagatcgc gagaatctga gcaatttcgg
     2701 cgaaggtgag gtcgttccag attttcaaaa cgacaacctc ctgttgttcg tcaggcaaac
     2761 gctcgacggc cgccgcgagc aattgctggc cttcatccag ggtggtgaat tgcggctccc
     2821 aatgggtggc gccatcgagc gcgaccgtgg cttcgcgccg cgcccggcgc tggtcactgc
     2881 gcaaacgatc gagcgcggtc gagcggacgg tggcgtagag cagcgcgcgg ttttcgggcg
     2941 aatgctggcg ccgccaaaaa cggacgaacg catcctgcac gatgtcctcg gcgtcagcgc
     3001 gcgaatttgt ccagcggcgc gcgaaaagga gaagcgcggg gccgagctgt gcgaaacagg
     3061 ttttccaatc ttcatcggta agcgtactct ccatgacctc ttcacggtct tttaatatgg
     3121 agacgccgcc aaatgcagtt tttgtctagc cgggtgaaac ttccggccgc gctgctctcg
     3181 ctctgcgccg cggccacttc tttggcggaa gttccagcga cggacggcaa cgcccggtcg
     3241 ctcgccgcag cggaatctgc tttcgcccag gaagcactcg ccagggggac gcgccgcgcg
     3301 tttctcgacg cgctctccgc cgatggcgtc gtcttccagc ccggaccgca aaatggccgc
     3361 aaagtttggg aagcgcggaa ggattcaggc ggtattctgg catggcaacc ggtgctggcg
     3421 gtcgtcgcgg cgagcggcga tttcggttac accaccggtc cctggacttt caaaaaaacc
     3481 gcggcggacg agcaacccgc tgcgttcggg caattcgttt ccatctggcg gcgggaagcc
     3541 gggaaatgga aactgctttt cgatctcggc accgaaaatc cagcgcccac aacgccggcg
     3601 ccagccctgc ggttgcgcga taatcgtctg cctaacgaat cggccgcggc ggcacgcgcg
     3661 actttgttgg agcaggatcg gagctatgcc gcggaccgct tgaagaattt ttccgccctg
     3721 gccgcggacg atgttcgtct ctacctgccg gcgaaagttc cggtggtcgg gaagaccgga
     3781 gcggtcgccg ctctccagga agcggccggc acgacgacgt tacgcgagcc gaaagcagaa
     3841 gttgcgagca gcggcgacct cggcttcgca tggggcgagt atgccgccgg aaacgccgcg
     3901 gacgcgaccg gctactacct ccatatctgg tgcaagaatg agcagggcca gtggcggctc
     3961 gcgctcgatt tgctctcccc tcgttaggcg aacgaaccgg catgcaacca gcctctctca
     4021 tgccgtcggt ctcgatcatc gttccgacgc tgaacgaagc ggagaacgtc gaaccgctga
     4081 cccgacagat catggcgagc ggcattcatc cgctggagat tattttcgtc gacgatggct
     4141 cgaccgacgg aactggcgac cgggtgcggg agctggccgc ggaatttccc gttcgcctcg
     4201 tggtgcgcga aaatcccgct ctcggtctct ccggggcggt cgtggccggc gcgcaggacg
     4261 cgacgggcga tctcctcgtg gtgatggatg ccgacctcag ccacccgccc tcccggattc
     4321 ccgacctcgt taggccgttg caagcgggcg cctgcgaaat ggttgtcggc agccgttacg
     4381 tccgcggcgg caccacgccc ggatggccgc tctggcgcaa gatcatgtcc cgcgccgctt
     4441 cggccttcgc ctatccgctg acccgggtcc acgattcgat gtgcggcttc tttgccatcc
     4501 cgcggccgct gctgctcgag cgggcgccgg ccgcgacggg attcaagatc gctttcgaga
     4561 tcatcgtgca cggcgagcgc cgtctccggg tgcaagaaat cccaatcgcc tttcgcgacc
     4621 gggcgcgggg cgcgtcgaag atgaccttcc gggtcgccgc cgtctttttc gttcgctggc
     4681 tggccgcggt cgtcaggcgg aaaccggcgc gcgccgcagc cgccgcagct cgagcgccag
     4741 tccgataaaa agcaggaggg cggcgaaaag agtgttgccg taggactgcg accagtgctg
     4801 aagcgggatg gtcaaggcga gcaggagaac cgccgccagg gcccaccagc ggagcggcga
     4861 gcctggccta acgagccaga cgcgcgtgat gatggcgaac gggagaagaa ggaaagcgaa
     4921 gagataattg aaggagagcg gcgcggcgat gagcaacatg agaaggagca tcgaccactc
     4981 gatggcgtcc gtttcccagg tgcgccccgc gcccggtggc atcgccccga gataaaccag
     5041 gccgaggagc aaagagagcg cgacgatgat ggcgttgacc accttgaagg ggaggtcgat
     5101 aaagttaaca aagatcggtt tgtccggcgg acgcgtggca tccacgtcca ccttgcgcag
     5161 gaaccgattc gccaccccgg cgagcgattg gtttttccaa gcgacgctcc gctcaggccg
     5221 ctgcgcgatt cccttctcgt catattggaa gagcatgccg tccgtccagc gagtaagatc
     5281 ctggcgcgct cccgcccagc cccgaaatcc ggccggcaag acgaggagca ggagcgcgag
     5341 cgagagcagc atcgagagcg ttgcgatcca gtatcgccgg taaatcaggt aagcgatggc
     5401 cgccaccggg aacgccttga tcgcggcggc gagggcgaac attgtccccg cactccagga
     5461 ccggcccgca cgcaacgcca gaaacccggc gaggagcaga gcgagcagaa ccagggtcgg
     5521 ttgcccgagc agatagcaac tccagacgta aaccatgatg acgagattgg gcacgaggta
     5581 gagcatggca tgcgggcgtt cacccccggg gttcgccagc cggagcgacc agacgatgca
     5641 ggcccaccag gcgaggctat tcaccaggac gagcgcggcg atcaaaccgg cctggcctaa
     5701 caagctcagg ggcgcgagca tttccgcggc cgtcggcggg tacatgaacg ggaaacgctg
     5761 accctgtggc ggataaatgg cacctccgtg caggacggtc tgcccggtgc tgaaccacag
     5821 cttgtagtct ttggcgccgc gccccgcggc caggttgacc agcggcacga tcgaaaacac
     5881 aatcgtgagc agaatgaaga ggacgacgag caggcgaaag ccggtgcggc tgttcagcca
     5941 atcgaggaat cgtgccatcg tttatttcgt gcgcgccgcg gggcgcagca tcaaatcgcc
     6001 cagccaatag ggatccgcca ccgcttttcg gtaggcggcg agcgcttcgg gcgaagaatt
     6061 gatgtgcaga ttgctgtagg actggtcgtc gttctgccag cgctcatgcc aatagatctc
     6121 cgctttcacc cgcgggcatt tggtgcgcat gacttcaaag gcgtcgcgca tccactgcgc
     6181 cttgtttccc gaattgggga attcgccggc ggcccattcc gtgatcatga tcggtttctt
     6241 cggatcgagc gcggcgattt cccggtaggg ccaatcgagc aacggcaaaa aattcgacca
     6301 cggctcatcg tcgaattgct ggccgtagac gcttaagccc agccagtcga cgtaatccgg
     6361 gccgggataa taggcggcgg cgagattcca ggtgtccatc gggaacgaat aattattcgt
     6421 ctggaaaatc cactggatgt tgtgcgcgcc gcgcgcccgg acgcggtcca ccacatagcg
     6481 aaagacgcgc ttgaaaaggt cgggtccctc ccattcgtta ggccggccgg gaatcttttt
     6541 cccgccgccg taataaatgc ccgaccaggg aaaccagtcg ccattcatct cgtcgccgaa
     6601 cgagacgagc aacgggtggc caaaggcgcg cgccgcgtct gcccagtcgt cgatgtagcg
     6661 attccaggtg ccggcaagga tcgcgttgag accaaaccgg tcgggtccct tcttctggtc
     6721 gtagggtcga tcccacggcg accagaaaac gagcggaaga gagccgtgcc gccagatcat
     6781 ttgcagattc gcggtgggaa aactctgctc cccccaatag ctcgacgagg cgataatcgc
     6841 ctggtgtttg ccgaccatct gctcgaagtc ttcgatcgtt tcgag
//
LOCUS       DATMNS010000034        18865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5053506, whole genome shotgun sequence.
ACCESSION   DATMNS010000034 DATMNS010000000
VERSION     DATMNS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 18865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18865
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5053506"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="VNW28_01285"
     CDS             complement(<1..348)
                     /locus_tag="VNW28_01285"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXA08582.1"
                     /translation="MDLEEDHALTEKIIGLAMKVHRTLGPGFLESVYLNALAYELRRA
                     GLEVEIGQRIKVRYGNVIVGDFVADLVVAGTLICELKAISSLTKADEVQVVNYLTATN
                     HEVGLLLNFGGTSL"
     gene            complement(373..2943)
                     /gene="gyrB"
                     /locus_tag="VNW28_01290"
     CDS             complement(373..2943)
                     /gene="gyrB"
                     /locus_tag="VNW28_01290"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF011501.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HXA08583.1"
                     /translation="MLQTNDETALSSSLSERPAAISENQKYNAAQIDKLEGLEAVRKR
                     PGMYIGDPDERGLHHCVFEVLDNSIDEHLAGYCSRIDVAIHVDGSVSVRDNGRGIPVD
                     MHPKFKMPAVELVLTNLHAGGKFGQGAYKYSGGLHGVGAKCVNALSDWFKVEVSRDAK
                     VYHMAFERGKTTEKLSVIGELKNKKTTGTLITFLPDPTIFTITTEFKFERLASRLREL
                     AFLNPGLEITLTDERTEPPKKETFYYKHGIEEFVKQLGENKTVIHPKPVIISRQRDEV
                     FVDVVLQYNDSYNDQILPFANSIPNPDGGTHLTGFRTALTKAVNQYAKANSLVKEKDP
                     AISGDDVREGLICVLSLKLPNPRFESQTKVKLVNTEIDGIVNSVVYDGLMTYFDQNPP
                     IAKRIFDKVLTAARAREAARKARETIRKGALTGGGLPGKLADCSERDPELTELYIVEG
                     DSAGGSAKQGRDRRYQAILPIRGKLINVEKARLDQALKNTEIQSMITAIGTGIGKPKE
                     EATTKEEGTFDISKLRYGRIIIMTDADVDGSHIRVLLLTFFYRQMTELVRAGKIYIAQ
                     PPLYQIKRKKREEYVDDDVQLNKILISLGAEDVRLKNLADNKELTTAQLKDILEQLEK
                     LAKLSESVRRHGGDFENFLSHRDKKADKLPTYLVKVRDGNDESVHYFHDERSVRKFHE
                     DNLDLNLFDVEMGQELLPLTSAPAKTNGNRRRGKLVELHESTTIQKLISELARKGLKV
                     DHYAAADKPIFELLEGEGERATSHPLFSIPEILEKIMEIGRRGVQIKRFKGLGEMNAK
                     ELFNTTMDPERRKLLKVDLNDDNAVEADQMFSKLMGDVVEPRRQYIEDNALNVRNLDV
                     "
     gene            complement(3657..6032)
                     /locus_tag="VNW28_01295"
     CDS             complement(3657..6032)
                     /locus_tag="VNW28_01295"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HXA08584.1"
                     /translation="MFNPIQRYTHWLHTRWPAGVVEKLPESGEFGVTNIPGVRIVGDL
                     TGIPLLKFSSKTGAEAVRAILQEPDFVTSPTTTGETLDLAIIGGGVSGVSAAIEAKKA
                     RLNFRIFEATQPFSTVVNFPKAKPIYTYPTDLKLEGGLQFTKDVKEALLEEMEEQRKA
                     AGIEFTDARIERLEARGKEILLHHGDKTRTITRARRVIVAIGRSGNHRKLGCAGEDLS
                     KVYNRLYDPKDFSGKNVLVVGGGDSALECAIALAGSGAKVTVSYRKPEFARPKPENVE
                     KLKMLEDNPKANVQVERPSSERVTTAFTSEMMRNAPPGSVTLALATAVTRIEPDKVIL
                     KKEDGTEAALPNDVVFSMIGREAPLEFFRRSGLHVRGDWRPITWISCLAFVLFCVVLY
                     HWKSNHPQEFPVQRWASQHHAFPYNVPKAIDSAGGKIAAWSSRETNLLYTIKRGLGNP
                     AFYYTLAYCTCVFVFGIRRIRRRRTPYVQWQTIVLICSQCIPLFILPELILPWMGHNG
                     FFEPGHPLRWLADHLFESYDGSLGHERAYWRAYGFILAFPLNVYNVFTDHPMWLWLGI
                     CFVQTFVIIPLIIFRWGKGAYCGWICSCGALAETMGDTQRSKMPHGPFWNRVNMVGQV
                     ALGFAFAILGLRIAGWALGEDSWPTHWYHKLFEGIPFLNYAWSVDIFMGGILGVGLYF
                     WFSGRVWCRFACPLAALMHIYTRFSRYRIFPDKHKCISCNVCTSVCHQGIDIMNFANK
                     GLPMEDPECVRCSACVQQCPTGVLSFGRYDGERNIILDKLAASPVLMREER"
     gene            6209..8101
                     /gene="treZ"
                     /locus_tag="VNW28_01300"
     CDS             6209..8101
                     /gene="treZ"
                     /locus_tag="VNW28_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HXA08585.1"
                     /translation="MNHRPFQRRFPIGAEVSAPGRTHFRVWAPKARRIDLVIETDVAP
                     EAERIFQPLAAEGAGYFSGQADGAAGTRYRFRVNESENFHPDPASRYQPGGPHRSSCV
                     VDHRDFRWSDQKWPGVELKGQIIYELHVGTFTPEGTWAAAARQLPELARTGISVIELM
                     PLADFPGEFGWGYDGVALFAPCRLYGTPDGLRGFVDAAHSLGIGVILDVVYNHFGPEG
                     NYLRVFSDDYFTERYETDWGDAINFDGPNSGPVREFFISNARYWIEEYHFDGFRFDAT
                     QSIFDQSAEHILGAIGKVARAAAGRRSIILVAENETQETRLVRPAAEGGYGLDALWND
                     DLHHSAVVALTGRNPAYYTDYRGTPQEFISAVKHGYLYQGQHYRWQKMRRGTPARGLG
                     PEAFVGFIENHDQVANSLSGERVRRTTSPGRYRAWTALLLLAPWTPMLFQGQEFGATT
                     PFLYFADLTGDLREKIRAGRLTFLKQFPALASARAQTELADPSEPATFERSKLDFSER
                     EKHPEICALHRDLIKLRRSDANFARQERGQVDGAVLGAHCFILRFFDSLGDDRLLIIN
                     LGVALALEVAPEPLLAPPGPGRWKTLWSSEAPRYGGPGQVALETRKGWTIPAEAAVAL
                     RPVRSA"
     gene            8115..10196
                     /locus_tag="VNW28_01305"
     CDS             8115..10196
                     /locus_tag="VNW28_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HXA08586.1"
                     /translation="MNLDRLRELPKPRVMPWGPEQPSPESLLTREWLATNGLGGFASG
                     TISGAVTRRYHGLLVAALPAPLGRTVMWSHVSEVLRFGEHDEVSLGAEERARGQLNLE
                     GADHFKEFRLEKGLPVWIYEVRGLTIEKRVMMPHRQNTVHVSYRVSGPGQRPRLDVRP
                     AFNFRHYEDRVDRLPEALYEIRAKDGRYEVLDGERKFPPLRMRLSDCDCDFLIEPRKI
                     REVVYRTELDRGYDYEGDLWSPGIFRIDLEEKGTATLVGSTEEWNILDVLTPQAAQMA
                     ELSRRVRLLEQARPSAQDSVGAELILAADQFVITPAGRAEEEARAHAAGDEVRTVIAG
                     YHWFTDWGRDTMISLEGLTITTGRALEAGYILRTFAHYVRDGLIPNMFPDGEKEGLYH
                     TADATLWFFHALGRYLAATGDRATLESLLPTLCDIVEHHLRGTKFNIHVDPKDGLLIQ
                     GKEGYQLTWMDAKMGDWVVTPRRGKAVEINALWYNALELLAGWLHETGDEEAANRYRE
                     HAQRAHRSFNERFWHKEGGYLYDVLDGPEGEDNSIRPNQVFALSLDHPVLQKDRWAPV
                     LAVVERELLTPVGLRSLSPRNPEYKKNYHGDLKTRDAAYHQGTVWAWLIGPFIDAWLK
                     LHPDELAKARDFLRAFPAHLDEAGLGTISEVFDAETPHTPRGCIAQAWSVAEVLRCWV
                     KTEGVAPGNAR"
     gene            10281..10616
                     /locus_tag="VNW28_01310"
     CDS             10281..10616
                     /locus_tag="VNW28_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HXA08587.1"
                     /translation="MICVTDKAVNHLRSLLAEQPAAAGKGLRVQVAKGGCSGMQYEMS
                     LDERHAGDAVVQRAEVEFLIDPESADFLREATLDFEDGLTGAGFRIVNPNAARTCGCG
                     TSFEPVRPA"
     gene            10841..12409
                     /locus_tag="VNW28_01315"
     CDS             10841..12409
                     /locus_tag="VNW28_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08588.1"
                     /translation="MAWNPDLFRFDDHHGFERKKTNFFAWTIAILLLSGVALAAWLGS
                     LYVLGQPERPESYRLLQKLHKIEPPKRFALTAAPAGEFLNPKDLFARYQAMGPAELAR
                     KDAELTRNFIRNFQQVHGLVTYVVGRFNIVGARELTKDDVFTSGAVALARAVEQPELL
                     MEHVFTTANEHDARLLEERLVMGMDVNFERSHDLSAVVHVERLADGRVQITAIPLLYG
                     SYAVTRGTGTFSLEPPADLNLAAGWPVFKKEMRRTAETRYDSYRSRLAPASSSLPIAG
                     LPPPKTAPPSEDALVRVEPAVPLEEEPPPPPLRRPGRTKPALVRAWKKEKGLASSSPI
                     VARRARPVPPDAAFSPNEGTSLAAASPAASPMVMRALPAPSSSPAVALTSTTGATWKT
                     FAPGQAPAGRLLDAAGLREVANNGTRGERIYLKGQFVVNFADANRAVLRPTRDQSDQR
                     SGGLGRNVRIIVDYPAGAPLPDPGSTLNRDSGRPYEVTEVRQQSDGQLNVFVREIIRP
                     QARAATADIAEPDQ"
     gene            complement(12403..13095)
                     /locus_tag="VNW28_01320"
     CDS             complement(12403..13095)
                     /locus_tag="VNW28_01320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08589.1"
                     /translation="MATDSTNPSWIEPPPEKRGGLGCFGKSCLALAAIALIFLLVLGI
                     GSYSFFARGSKPASLPIEELPQKALSDVQQRVEKFRAPPPLPLPPPAAVEPGATPAPT
                     PVPQQPEKELVMSAAEINGMISANPKSRGHAYVSLNGHTATVQISVPSDKVPNFPRGY
                     LNGSFAITTDGPTTIGALQVSKIEANGIPVPSSILSMSYRGNSILGYALEAIAPYSVS
                     TAEVRDGNVYLH"
     gene            complement(13100..13609)
                     /locus_tag="VNW28_01325"
     CDS             complement(13100..13609)
                     /locus_tag="VNW28_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HXA08590.1"
                     /translation="MEFPKPEIEALWCPWRVEYFEIEKRNQNFLSEAAQASDDAAHLV
                     ITRRKNVFLMMNRYPYSVGHLMVVPYRKTAELSSLGENEILELWALAVHAQTLLRETM
                     KAQGFNLGLNLGTPAGAGVADHLHLHIVPRWEGDTNFMPVLGRTRLLSEGLQTLYEKL
                     MAAQSRLAG"
     gene            complement(13721..15169)
                     /locus_tag="VNW28_01330"
     CDS             complement(13721..15169)
                     /locus_tag="VNW28_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08591.1"
                     /translation="MSGKGEWLAQPRTRWVVLSLIMVFFASTNVPWTLDDYDQAKQAY
                     TSFEMVGQGHWLYQHTPNESVATKPPLVGWISAALFALTRWWEGAWRIPSFVAALALL
                     WLIAREATAAYGAAGGLIALSAFGLNLLAPRLATLVRTDMPLALATFLIGGQIWQKIH
                     RAEPWATRDRVIAFLLLTSAMLIKGPIIYAFILPGLIVYQIRRRKSGAPSGWSGWWPW
                     LLSLAIFLAWAIGGIVRQTGFYDSVVIREFAGRFTETIHKPQPIYFYLPHLLHKFAPW
                     SVLLIAFAIAFWRRRDERWKMQPATLWLVCWSVGGLILMSVIPSKRVDRIFPLVPPLC
                     LLLGAQVAAALRIEPWRGRLWRWLATALIFAGIFTGGYVAWKIGQGYHSRSAALVQFS
                     QQVREKAEQHHWRYAVVGGREEGMLLYLRRDHFLAPDDAVRQWNAGLLDALVVRDEPA
                     RPWLRLLSGAQLAFASEKSADVPQYSLFVRGM"
     gene            complement(15173..16567)
                     /gene="creD"
                     /locus_tag="VNW28_01335"
     CDS             complement(15173..16567)
                     /gene="creD"
                     /locus_tag="VNW28_01335"
                     /inference="COORDINATES: protein motif:HMM:NF008712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HXA08592.1"
                     /translation="MPEMQPPPPLPRSQPFLRRNRTVIKLFGVGALILILLIPLAMIH
                     GVLSDRLERRNEAVSEITDSWGKEQNILGPVLGVPFQYKFRSMKDTTMPDGHVERREV
                     EETLVANAFFLPESLQITGDAQTQTLHRGIYNAAVFRAQVKLSGKFAPPDFGALKIDL
                     KDVQWKDAFVTIAVSDLRGTREGLVLDWNGTKRSLQPGAQLPGYTTGATALLGLDHPL
                     ASAADFAISLDVNGSGGIYFAPFGVRNEAELKSNWPDPGFRGAFLPAERSVRPDGFAA
                     KWKVSYYGRDYPQSWTSIRGNQRFNEKTVAASFFGAEFLSILDAYRFVERSIKYGVLF
                     LVLVFTTFFLFEVTARQKIHPFQYLMVGAALCLFYLLLLSISEFIGFGLAYLIAAVAA
                     TVLITWYCNFFLGGGVRTLMIGAGLAGVYTFLYITLRQQDYALLMGAIALFILLAVVM
                     YVTRKVDWYARDVA"
     gene            16663..17019
                     /locus_tag="VNW28_01340"
     CDS             16663..17019
                     /locus_tag="VNW28_01340"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXA08593.1"
                     /translation="MLVAVNKQENPWIEIAPGIKRRTLAAGPSMYQMRAELAAGSRLP
                     EHMHPQEQIAHLIKGRIKLIVAGVPHELAAGDVFYLGSNLPHGVETIEDTTVIDTFSP
                     PRDDYLALDEKARRAG"
     gene            17020..18189
                     /locus_tag="VNW28_01345"
     CDS             17020..18189
                     /locus_tag="VNW28_01345"
                     /inference="COORDINATES: protein motif:HMM:NF024106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative molybdenum carrier protein"
                     /protein_id="HXA08594.1"
                     /translation="MKIITGGQTGVDRAALDAAMSLGVAAGGWCPAGRLAEDGVIPPQ
                     YPLQELPGGGYPERTERNVREADGTVIFHSGLLEGGTKATADFCAEMQKPCCSIDARA
                     ATPEETARQLAEFVRANQIRVLNVAGPRASQWPPGYDFTGQTLDRLLRPAPKLSFVVP
                     AHNEEHELPASLNALRAAADASGQSYELIVVDDASTDATAAIAQQFGARVIPVNRRQI
                     AAVRNAGAREARGEILFFVDADTRIAPDHVILGVAALAEGCAGGSARVVPDREMPFWA
                     SVFLQVFSAVYFTAGLGAGAFIFTRHASFEAAGGFDEQYFAGEEVYLSLALKKLGQFR
                     ILREPIVTSARKVRMHSPRFFLTQTFFLLFGGKRALRSRDRLGLWYDGKRERHAN"
     gene            18248..18784
                     /gene="nbaC"
                     /locus_tag="VNW28_01350"
     CDS             18248..18784
                     /gene="nbaC"
                     /locus_tag="VNW28_01350"
                     /EC_number="1.13.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyanthranilate 3,4-dioxygenase"
                     /protein_id="HXA08595.1"
                     /translation="METKVKPSIDLANWIDEHKDKFKPPVSNQYLYDGRDFFVMVIKG
                     PNARNDFHLVDSEEYFYQLKGNIKVRIREGDQIVDHLVREGETFFIPPNVPHAPVRPP
                     DTIGVVVERRRPPGEHEHVIFYCEKCGALVEDIDFDCGDIVQHFSQAMLDFWNDDARR
                     TCRKCGTKVEQPGPTKPF"
BASE COUNT         3647 a         5760 c         5817 g         3641 t
ORIGIN      
        1 aagactcgtt ccaccgaagt ttaggaggag gccgacctcg tggttggtgg cggtaaggta
       61 atttacgact tgcacttcat cggctttcgt taggctggaa atggctttca actcgcaaat
      121 caacgttcct gcaacgacca aatcggcgac gaaatcgccc acgatgacat tgccgtagcg
      181 taccttgatt cgctgtccga tttccacttc caagcctgcg cgtcgcaact cgtaggcgag
      241 cgcgttgagg tagaccgatt ccaagaagcc gggcccaagt gtgcgatgca ctttcatcgc
      301 aaggccaatg atcttctcgg tcagcgcgtg atcttcttcc aaatccaatc ccgtaaatcc
      361 tgttaatcct gtctaaacat ccaggttgcg cacgttaagc gcgttatcct cgatatactg
      421 gcgccttggc tcgacgacat cgcccatcaa cttggaaaac atctggtcgg cttcgaccgc
      481 gttgtcgtcg ttcaggtcga ccttcagcag cttgcgccgc tcgggatcca tcgtggtgtt
      541 gaacagttcc ttggcgttca tttcgccaag acctttgaaa cgcttgatct gcacgccgcg
      601 gcgcccgatc tccatgatct tttccagaat ttccggaatg gaaaaaagcg ggtgcgaggt
      661 cgcgcgctcc ccctcgcctt cgagcaactc gaagatcggt ttatccgccg ccgcgtagtg
      721 atccactttc aaacccttgc gggccagctc gctgatcagt ttctggatgg tggtcgattc
      781 gtgcaactcg accagcttgc cgcgccgccg gttgccgttc gttttcgccg gagcgctcgt
      841 cagaggaagc agttcctgcc ccatttccac gtcgaagaga ttgaggtcga gattgtcctc
      901 gtgaaacttc cgcaccgagc gctcgtcatg aaaataatgg accgactcgt cgttcccatc
      961 gcgcaccttc accaggtagg tcggcaactt gtccgctttc ttgtcgcgat gactgaggaa
     1021 attctcgaaa tcaccgccgt gccgccggac cgattcgctc agcttggcca gtttctccag
     1081 ctgctcgagg atgtccttca gttgcgccgt cgtcagctcc ttgttgtccg ccaggttttt
     1141 cagccggaca tcctccgcgc ctaacgagat cagaatcttg ttcaactgca cgtcgtcgtc
     1201 cacgtattcc tcgcgtttct tgcgcttgat ctggtagagt ggcggctggg caatgtagat
     1261 ttttcccgcg cgcaccagct cggtcatttg ccggtagaaa aaggtcagca aaagaacgcg
     1321 aatgtgcgaa ccgtccacgt cggcgtcggt catgatgatg atccggccgt agcgcagctt
     1381 gctgatgtcg aaggtcccct cctctttggt cgtcgcttcc tctttcggct tgccgatgcc
     1441 ggtgccgatg gcggtgatca tcgactggat ttcggtgttc ttgagcgcct gatcgagccg
     1501 cgctttctcg acgttgatca acttgccgcg gatcggcaga atcgcctgat aacgtcgatc
     1561 gcgtccctgc ttggcggagc cgccggccga gtcgccttcg acgatgtaga gctcggtcag
     1621 ctccggatcg cgctccgaac aatcggccag cttgccgggc aacccgccgc cagtgagcgc
     1681 gcctttgcgg atggtctcgc gcgccttgcg ggcggcttcg cgggcgcgcg ccgctgtcag
     1741 caccttgtcg aaaatccgtt tcgcgatcgg cgggttctga tcgaagtaag tcatcaaccc
     1801 gtcgtagaca accgagttca cgatgccgtc gatctccgtg ttgacgagct tcaccttggt
     1861 ctgcgactcg aaacgcgggt tgggcagctt gaggctgagg acgcagatca gcccctcgcg
     1921 cacgtcatcg ccggagatcg ccgggtcttt ttctttcacc aggctgttcg ccttcgcgta
     1981 ttggttgacc gctttcgtca gggccgtgcg gaagccggtc aaatgggtgc cgccatccgg
     2041 attcggaatc gagttggcaa agggaagaat ctggtcgttg tagctgtcgt tgtactgcag
     2101 gacgacatcg acaaacactt cgtcgcgctg gcgcgaaatg atgaccggct tggggtgaat
     2161 cacggttttg ttttcgccca gctgtttcac gaactcctcg atcccgtgct tatagtaaaa
     2221 cgtctctttc ttcggcggct ccgtccgctc atcggtcagg gtgatttcca gcccgggatt
     2281 gaggaaagcc agttcgcgca gccggctcgc caggcgctca aacttaaact ccgtcgtgat
     2341 cgtgaagatc gttggatccg gcaggaaggt gatcagcgtg ccggtcgttt tcttgttttt
     2401 cagctcgccg atgacgctca atttttcggt cgtcttgccg cgctcgaaag ccatgtggta
     2461 gactttcgcg tcgcgcgaca cttcgacctt gaaccaatcc gacaacgcgt tgacgcactt
     2521 cgcgccgacg ccgtgaagac cgccggaata tttgtaggcg ccctgcccga atttgccgcc
     2581 ggcgtgaaga tttgtcagca ccagctcgac cgccggcatc ttgaatttcg gatgcatgtc
     2641 gaccggaatg ccgcggccgt tgtcgcgcac cgagaccgag ccatcgacgt gaatcgcgac
     2701 atcgatccgg ctgcaatagc cggccagatg ttcgtcgatc gaattatcga gcacctcaaa
     2761 aacgcaatgg tgcaatccgc gctcgtcggg gtcgccgata tacatgcccg ggcgtttccg
     2821 gacagcctcg agaccttcga gtttatcgat ctgggcagcg ttgtattttt gattttcgct
     2881 gatggccgcg gggcgttcgg aaagcgagct ggaaagggcg gtttcgtcgt tggtttgaag
     2941 catagaaccc gcgggtggtc cgcgagccag aaacataggc gaaaagaggg ctccggacaa
     3001 ctaattatct gaagccggcg aaaacctcgc agccgctaga tttagcggga gccggcgggg
     3061 aggcgcccag atgtagtggt cgctccgggg tgctcaattt cctgttgccg gcggatgaca
     3121 gaactcccgc cacgccccat gctggagccc gatctcgccc ttgggagctt atcctaaccg
     3181 gcttcctgcg acttctcagt cggcgcccat cgccggattc aaagccggcc ctttagacag
     3241 cgcagaaggg acgcgccggc cgccggcggt tattcggatg ctccattcca gctcacccct
     3301 aacgaggcga agaggaatta catccttgcg cggtgcggtc catgctaatt catagattcg
     3361 ttagccgacg cgtggagcgc gtcacaggaa tttaggattg ttctgggggg aactgcccgc
     3421 gttgccgatt cggtggcgcg ggtcttcctt ttttggggct gattctctct tcgcagagct
     3481 cgtcggattg tcagtcacgg gctgctgtcg gttgcaggtt gcctgaggca ggtgaccccg
     3541 ttcgcgcaca gctctgccga tgctcgtcca gccgggatcg ccggtcccgg ctacaactcg
     3601 cgcttgagat acgaacgacc cgaccaaccg acaagcaacg aacgcgacgc tctctcttac
     3661 cgctcctccc gcatgagcac gggagaggcg gccagtttat cgaggatgat gtttctttcg
     3721 ccatcgtagc ggccgaagga gagcaccccg gtcgggcatt gttgcacgca ggctgagcag
     3781 cggacgcact ctgggtcttc catcggcaga cctttgttgg cgaagttcat gatgtcgatc
     3841 ccctgatggc agaccgaagt gcagacgttg caggaaatgc atttgtgctt gtcggggaaa
     3901 atccggtagc gcgagaaccg cgtgtaaata tgcatgagcg cggcgagcgg acaggcaaag
     3961 cgacaccaga cccgcccgct gaaccagaaa taaagcccga ctccgagaat accgcccatg
     4021 aagatgtcga cgctccaggc gtagttgaga aagggaatgc cctcgaaaag cttgtgatac
     4081 caatgggtcg gccatgaatc ttccccgagc gcccagccgg cgatgcgcaa gccaaggatc
     4141 gcaaacgcga aaccgagcgc gacctgcccg accatgttga cccgattcca gaagggcccg
     4201 tggggcattt tcgagcgttg cgtgtcgccc attgtctcgg cgagcgcgcc gcaggagcag
     4261 atccagccgc agtaggcgcc cttgccccag cggaaaatga tcagcggaat gatgacaaag
     4321 gtctgcacga aacaaatgcc gagccagagc cacatcgggt ggtcggtgaa gacgttgtag
     4381 acattgagcg gaaaagcgag gatgaacccg taagctcgcc agtaagcgcg ctcgtgccct
     4441 aacgaaccgt cgtaactttc gaacaaatga tcggccagcc agcgcaacgg gtggcccggt
     4501 tcgaaaaatc cattgtgtcc catccagggc aggatcaact ccgggaggat gaagagcggg
     4561 atgcactgcg agcagatgag cacgatcgtc tgccattgca cgtaaggcgt gcgccgccgc
     4621 cggatgcgcc ggatgccgaa gacaaagacg caggtgcagt aggcgagtgt gtaatagaac
     4681 gcggggtttc cgagcccacg cttgatcgta taaagcaggt tcgtttcgcg gctcgaccac
     4741 gcggcgatct tcccgccggc ggagtcgatc gcttttggca cgttgtaggg aaaagcgtgg
     4801 tgctggctcg cccagcgctg gacgggaaac tcctgcggat ggttgctctt ccagtggtag
     4861 agcacgacgc aaaagagcac aaacgcgagg caactgatcc aggtgatcgg ccgccagtca
     4921 ccgcgcacat gcaatccgct gcgccggaaa aactcgagcg gcgcttcccg gccgatcatg
     4981 gaaaagacga cgtcgttagg caaggcggcc tccgttccgt cttccttctt caggattacc
     5041 ttgtccggct cgatccgggt caccgccgtc gccagcgcga gcgtgacgga acccggcggg
     5101 gcgttccgca tcatttcgct cgtgaacgcg gtcgtcaccc gttccgagct tgggcgctcc
     5161 acctgcacgt tcgctttcgg attgtcctcc agcattttga gcttctcgac gttctccggc
     5221 ttcggacgcg caaactccgg tttgcgatag ctcaccgtga ccttcgcgcc ggagccggcg
     5281 agcgcgatcg cgcattccag cgcgctgtcg ccgccgccga ccacgagcac gtttttgccg
     5341 ctaaaatcct tcgggtcgta gagccggtta tagactttcg acaggtcttc gccggcgcag
     5401 cccagcttgc gatggttgcc gctgcggccg atcgccacga tcacccgtcg tgcgcgcgtg
     5461 attgtcctcg tcttgtcgcc atgatggagc agaatttctt tcccgcgcgc ctcgagccgc
     5521 tcgatccgtg cgtcggtgaa ttcgattccc gccgccttgc gctgctcctc catttcctcg
     5581 agcagcgcct ccttcacatc cttcgtgaat tgcagcccgc cttcgagttt caggtcggtc
     5641 ggataagtgt aaatcggctt cgccttgggg aaatttacga ccgtcgaaaa cggctgcgtc
     5701 gcctcgaaga tcctgaaatt caatctcgcc ttcttcgcct cgatcgccgc cgacactccc
     5761 gacacaccgc cgccgatgat cgccaggtcg agcgtttccc cggttgtagt cggggacgtt
     5821 acaaaatccg gctcctgcaa aatcgcgcgc accgcttccg cgccggtctt ggaggaaaac
     5881 ttgagcagcg gaatgccggt cagatcgccg acgatgcgca cgcccggaat attggtgacg
     5941 ccgaattcgc ctgattcagg cagtttctcg accactccgg cgggccatct ggtgtgaagc
     6001 cagtgcgtgt agcgttggat cggattaaac atacgacgcg cgcccggcgc agtcttatcg
     6061 gagaaaagag gaggcgggaa gcgcatttat tcccgcttct gtcgccatca tcgttacgct
     6121 agcttgccct tttgcgaaca ccgaatcatc gaaacccaac gtcaaacccc gcggcgaccg
     6181 tcccatgcct ggtgggcgaa ttgagagcgt gaaccatcgt cctttccagc gccggttccc
     6241 gatcggcgcc gaagtgagcg cgccgggccg gacgcacttc cgtgtctggg cgccaaaggc
     6301 gcgccggatc gatctcgtga tcgaaacgga tgtcgccccg gaagcggaac ggattttcca
     6361 acctttggcc gcggaaggcg ccggctattt ctcgggtcaa gccgacggcg cggcgggcac
     6421 gcgctatcgc tttcgggtca atgagagcga gaattttcat cccgacccgg cgtcgcgcta
     6481 ccaaccgggc ggaccgcatc gttcgtcctg tgtggtggat caccgggatt tccgctggag
     6541 cgatcagaaa tggccgggag tggaattgaa aggccagatc atctacgagc tgcacgtcgg
     6601 gactttcact ccggagggaa cgtgggccgc cgcggccagg cagttgccgg aattggcgcg
     6661 cacgggcatc agcgtgatcg agctgatgcc gcttgccgat tttcccggcg aattcggctg
     6721 gggttacgac ggggtcgcgc tcttcgcgcc gtgccggctc tacggcacgc ccgacggcct
     6781 gcggggattc gtcgacgccg cccactcgtt aggcatcggc gtcatccttg acgtggtcta
     6841 taatcatttc gggccggagg gaaattacct gcgcgttttc tccgacgact atttcaccga
     6901 acgctacgag accgactggg gcgacgcgat taattttgac ggaccgaact ccgggccggt
     6961 gcgcgaattc ttcatcagca atgcccgcta ctggatcgag gaatatcatt tcgacggctt
     7021 tcgtttcgac gcgacgcaga gcattttcga tcagtcggcg gaacacattc tcggcgcgat
     7081 cgggaaggtt gcgcgggccg ccgccggccg acgctcgatc atcctcgtgg ccgagaacga
     7141 gacgcaggaa acgaggctgg tgcgcccggc ggccgaaggc ggctacgggc tcgatgcgct
     7201 ttggaatgac gacctgcatc attccgcagt cgtcgcgttg accggtcgga acccggccta
     7261 ttacaccgat taccgcggca cgccgcagga gttcatttct gcggtcaaac atggctacct
     7321 ctaccaaggg cagcattatc gctggcagaa aatgcgccgc ggcaccccgg cccgcggcct
     7381 ggggccggaa gcgtttgtcg gcttcatcga gaaccacgac caggtcgcaa actccctcag
     7441 tggcgagcga gtgcgccgca cgacttctcc gggccgttac cgcgcctgga ccgcgctcct
     7501 cctcctggcg ccctggacgc cgatgctttt tcagggccag gagtttgggg caacgacgcc
     7561 gtttctctac tttgccgacc tgacgggcga cttacgggag aagatccgcg ccgggcggct
     7621 gacttttctc aaacagttcc ctgctctcgc ctcggcccgg gcccagacgg agctcgcgga
     7681 tccgtccgag cccgcgactt tcgagcgcag caaactcgac ttttccgagc gggaaaagca
     7741 tccggagatt tgcgcgctcc accgggactt gatcaaactg cggcggagtg acgcaaattt
     7801 cgcgcgccag gaacgcggcc aggtcgacgg agcggtcctc ggagcgcact gttttatatt
     7861 gcgttttttc gactcgttag gcgacgatcg cctcctgatc atcaatctgg gcgtggcgct
     7921 cgcgctggaa gtcgcccccg aaccgctcct cgcgccaccc gggccgggcc gctggaaaac
     7981 gctctggtcc agcgaggccc cgcgctatgg gggtccgggt caggtcgcgt tggaaaccag
     8041 aaagggctgg acgattccgg cggaggcggc ggtcgcgctg cggccagtac gttccgccta
     8101 acgattttct atttatgaat ctcgatcgtt tgcgtgaact gcccaagccg cgcgtcatgc
     8161 catggggacc ggaacagcct tcgccggaaa gcctccttac ccgggaatgg ctcgcgacga
     8221 acggactcgg cggtttcgcc tccggcacaa tctccggcgc cgtcacccgc cgctaccatg
     8281 gcctcctcgt cgcagccctg cccgcgccgc tgggccgcac ggttatgtgg agtcatgttt
     8341 ccgaggttct tcgtttcggg gagcacgatg aagtttcgtt aggcgcggag gagcgcgccc
     8401 gcggccaatt gaatctcgag ggggccgacc attttaaaga attccggctc gaaaaaggtc
     8461 tgcccgtctg gatctacgaa gtgcgcggcc taacgatcga gaagcgggtc atgatgccgc
     8521 accggcaaaa taccgtgcac gtcagctacc gggtgagcgg ccctggacaa cggccgcggc
     8581 tggatgtgcg gccggcgttc aatttccggc actacgaaga tcgagtcgac cgtctgcccg
     8641 aggctctcta tgagattcgg gccaaggatg gccgctacga agtgctcgat ggggagagaa
     8701 aatttccgcc cctgcgcatg cggctttccg attgcgattg cgatttcctg atcgagccgc
     8761 gcaagattcg cgaagtcgtt taccggacgg aactggaccg tggttatgat tacgagggcg
     8821 atctctggag cccgggcatt tttcggatcg acctcgagga aaaagggacg gcgacactcg
     8881 tcggttcgac cgaagagtgg aacatcctcg atgttctaac tccgcaagcc gcgcagatgg
     8941 ccgaacttag ccggcgcgtg cgactgcttg aacaggcgcg cccttccgcg caggacagcg
     9001 tcggcgcgga gttgatcctg gcggccgacc aatttgtcat cacaccggcc ggtcgcgcgg
     9061 aggaagaggc gcgagcgcat gctgccggcg acgaagtgcg cacggtcatt gccggctatc
     9121 attggttcac tgattggggg cgcgacacca tgatcagcct cgagggccta acgatcacga
     9181 ctggccgcgc gctcgaggcc ggctatatcc tgcgcacctt cgcgcattac gtccgcgacg
     9241 gcctgatccc aaacatgttt ccggacggag aaaaggaagg tctctatcac accgccgacg
     9301 ccacgctttg gtttttccac gcactcggac ggtacctcgc cgccacgggc gaccgggcga
     9361 ccttggagtc gctcctgccg acgctctgtg acatcgtgga acatcatttg cggggcacga
     9421 agtttaatat ccacgtcgac ccgaaagacg gactgctcat ccagggcaag gaaggttatc
     9481 agctcacgtg gatggacgcg aaaatgggcg actgggtcgt cacgccgcgc cgcgggaaag
     9541 cagtcgagat caacgcgcta tggtataacg cgctcgagtt gctggccggc tggctgcacg
     9601 aaacgggcga cgaagaagca gcgaatcgtt atcgcgaaca cgcccaacgc gcgcaccgtt
     9661 ctttcaacga acgcttctgg cacaaggaag gcggttatct ctacgacgtg cttgacgggc
     9721 cggagggcga ggacaattcc attcgtccca accaggtctt cgccctctcc ctcgatcatc
     9781 cggtgctgca aaaggatcgt tgggcgccgg tgctcgcggt ggtggaaagg gagttgctca
     9841 cgccggtcgg gctgcgttcc ctttcgcctc gcaatcccga atacaagaaa aattatcatg
     9901 gcgatctcaa gacgcgcgat gcggcctatc accagggaac cgtttgggcc tggctgatcg
     9961 gaccttttat cgatgcctgg ttgaaacttc accctgatga actggccaag gcgcgagatt
    10021 ttcttcgcgc ctttcccgct cacctcgatg aagcgggcct cggtacgatc agtgaagtct
    10081 tcgacgccga aacgccacac acgccgcggg gttgcatcgc gcaggcctgg agcgtcgccg
    10141 aggtcctgcg ctgctgggtg aaaaccgagg gagtcgctcc gggcaacgcg aggtgaagcg
    10201 cgagtaaccc cctaacgagt gccccgcgtc ggaccccggc ccggttgact tacggacggc
    10261 tcacgatagc gtacgacagc atgatttgtg ttaccgacaa ggcggtcaac cacctccgtt
    10321 cgctcctcgc cgagcagccc gcggcggcgg gcaaaggcct gcgcgtgcag gtggccaaag
    10381 gtggctgctc gggaatgcaa tacgagatgt cgctcgacga acgtcacgcc ggcgacgcag
    10441 tcgtgcagcg cgccgaagtg gaatttttga tcgaccccga aagtgcggat ttcctgcgcg
    10501 aggcgaccct cgatttcgag gacggcctca ccggcgccgg ctttcgcatt gtgaatccga
    10561 atgccgcccg gacctgtggc tgcgggactt cgttcgaacc ggtccgcccg gcctagcgca
    10621 gttggtgcaa actggcggaa gttttctgga ggctggcgga atgacgaaat tccgaatgac
    10681 gagttccgaa tcaatgacaa agcaccaaga ccgaatgatc tggtgtcaga tggcgctcat
    10741 tcgtcattcg aacctgggaa ttgattcgtc attcgccatt cggaattcgt cctgcctccc
    10801 acctcgttag aagacgaccg cgcacgattt caatccgccc atggcatgga accccgatct
    10861 tttccgcttc gatgatcacc acggttttga gaggaagaag accaacttct tcgcgtggac
    10921 gatcgccatc ctgctcctga gcggagtcgc cctcgccgcc tggctgggca gtctctacgt
    10981 cctcgggcaa ccggagcggc ccgagagcta tcgtctcctg cagaagctgc acaagatcga
    11041 gccaccgaaa cgtttcgcgc tgacggccgc gcctgcgggt gagtttctga atccgaagga
    11101 cctttttgcg cgctatcagg cgatgggccc ggccgagctg gcgcggaagg acgcggagct
    11161 gacgcgcaac ttcatccgca acttccagca ggttcacggt ctcgtcacct atgtggttgg
    11221 ccggtttaac atcgtcggag cgcgggagct gacgaaagac gatgttttta cctcgggggc
    11281 ggtggccctg gcgcgcgcgg tcgagcagcc ggagctgttg atggagcatg tcttcaccac
    11341 cgccaacgaa cacgatgcca ggttgctcga ggagcggctg gtgatgggaa tggatgtgaa
    11401 tttcgagcgg agtcacgatc tgtcggcggt ggtccacgtg gagcgcctgg cggatgggcg
    11461 cgtgcagatt accgcgattc cgttgctcta tggcagttat gcagtgaccc gcgggaccgg
    11521 gacattcagc ctcgagccac cggccgatct gaatctcgcc gcgggctggc cggtcttcaa
    11581 aaaggagatg cgccgcacgg cggagacgcg ctacgacagt tatcgatcgc ggctggcgcc
    11641 ggcgagcagt tcgctgccga tcgccggcct gcctccgccc aagacagcgc cgccgtcgga
    11701 ggatgcgctg gtccgggtgg aaccagccgt gccgctggag gaggaaccgc cgccgccgcc
    11761 gctgcgccgg cctgggcgca ccaagccggc actggttcgg gcctggaaaa aagagaaggg
    11821 attggcctca tcgtcgccga tcgtcgcgcg ccgcgcccgg ccggttccac ccgatgcggc
    11881 cttctcgcct aacgagggaa cgtccctcgc ggccgcctcc cctgctgctt cgccgatggt
    11941 catgcgcgcg cttccggcac cctcttcgtc accggccgtg gcgctcactt ccacgacggg
    12001 cgccacttgg aaaaccttcg caccagggca ggcgcccgcg ggccggcttc tggatgcggc
    12061 gggacttcgc gaggtggcga ataacgggac acgcggagag cgcatttacc tgaaagggca
    12121 attcgtggtc aactttgccg atgccaatcg tgccgtcctc cgtccgacgc gcgaccaatc
    12181 cgatcaacgc tccggcgggc tcggacggaa cgtccgcatc atcgtggatt acccggctgg
    12241 cgcaccgctg cccgatccgg gttcgacctt gaaccgcgac tccggtcgtc cctatgaggt
    12301 gaccgaagtg cgccagcagt ctgatggtca actcaacgtc ttcgtgcgcg aaatcatcag
    12361 gccgcaggca cgcgccgcga cggcggacat cgccgaaccc gatcagtgaa gatagacgtt
    12421 accgtcgcgg acctcggcgg tgctgacgct gtagggagcg atcgcctcga gcgcgtagcc
    12481 caggatggaa ttcccccggt aactcatgga aagaatgctc gacgggaccg gaattccgtt
    12541 cgcctcaatc ttggacacct gcaatgcgcc gatcgtagtc ggcccatcgg tcgtgatcgc
    12601 aaaacttccg ttcagatagc cgcgcggaaa atttggcact ttgtccgacg ggacgctgat
    12661 ttgcaccgtg gccgtgtgac cattaagcga gacgtaagcg tggccccgag atttggggtt
    12721 ggcggagatc atgccgttga tctcggcggc gctcatcacg agttcctttt ccggttgctg
    12781 cgggacgggc gtcggcgcgg gagttgcacc cggctcgacg gctgccggcg gcggcaaagg
    12841 caagggagga ggcgcgcgga atttttcgac ccgttgctgg acgtcggaga gcgctttttg
    12901 gggaagctct tcaatgggca gcgaagccgg cttcgacccg cgcgcgaaga aggaatagct
    12961 accgatgccg agcacgagga ggaagatgag cgcgatggcg gcgagggcca gacagctttt
    13021 gccgaaacag ccaaggccgc cgcgcttttc tggcggcggt tcgatccagc ttggattggt
    13081 tgagtcagtc gccataaagt cagccggcca ggcggctctg cgcggccatt agtttttcgt
    13141 acaaagtctg gagcccttcg gaaaggaggc gagtccggcc caggaccggc atgaaattgg
    13201 tgtcgccctc ccagcggggc acaatgtgca aatgcaaatg atccgccacc ccggcgcccg
    13261 cgggcgtgcc cagattgagg ccgaggttga agccttgcgc tttcatcgtc tcgcggagaa
    13321 gcgtctgagc gtgcaccgcc agcgcccaaa gctcaaggat ctcgttttcc cctaacgacg
    13381 aaagctccgc cgttttgcgg tagggcacga ccatgagatg gccgaccgaa taggggtagc
    13441 ggttcatcat caggaagacg ttttttcgtc gagtgataac gaggtgggcg gcatcatcgc
    13501 tcgcctgcgc ggcttcggag aggaagttct ggttgcgctt ctcgatctca aaatactcga
    13561 cccgccaggg acaccagagg gcttcgattt cgggtttggg aaactccatg ggctcgttag
    13621 gcaaaacgct ccggcagcgt accggctgaa aaggagatgt cgagcgcggt tttgcggttg
    13681 ccgacgcgcc cgccgggcag cgcttcgcac agcgaagcgt ctacatcccg cgcacgaaga
    13741 gcgaatactg cggaacgtcc gccgattttt cggaggcgaa cgcgagctgc gcgccggaga
    13801 gcagcctaag ccaggggcgc gccggctcat cgcggacgac cagcgcatcg agcaggccgg
    13861 cgttccattg ccggacagca tcgtccggcg ccagaaaatg atcgcgccgc aggtaaagca
    13921 acatcccctc ctcgcgtccg ccgaccactg cgtagcgcca atggtgttgt tccgcttttt
    13981 ctcgcacctg ctgcgaaaac tgcaccaggg ccgcgctccg ggaatggtaa ccctggccga
    14041 ttttccaggc gacgtagcca cccgtgaaga tgccggcaaa gatcagtgcg gttgcgagcc
    14101 accgccacag ccttccccgc cacggttcga tccgcagcgc cgccgcaacc tgtgcgccca
    14161 acaacaggca gagcggcggc acgagcggaa aaatccggtc gactcgcttc gacggaatga
    14221 ccgacatcag gatcaatcca ccgacactcc agcaaaccag ccaaagcgtg gccggctgca
    14281 tcttccagcg ctcgtctcgc cgccgccaga aagcgatcgc gaaggcgatc aggagcacgc
    14341 tccatggggc gaatttgtgc agcagatgcg ggagataaaa atagatcggc tgcggcttat
    14401 ggattgtctc ggtaaagcgg ccggcgaatt cccgaatgac cacgctgtcg taaaagcccg
    14461 tctgcctaac gatcccgccg atggcccagg cgagaaagat ggcgagcgag aggagccacg
    14521 gccaccaccc gctccaaccg ctcggagcgc cactcttccg gcgccggatc tggtagacga
    14581 tcagccccgg caggataaac gcatagatga tcggcccctt gatcagcatc gcgctcgtca
    14641 ggaggagaaa cgcgatcacc cgatcgcgcg tcgcccacgg ctcggcgcgg tggatttttt
    14701 gccagatttg cccgccgatg aggaacgtcg cgagtgcgag cggcatatcc gtccgcacca
    14761 gcgtggccag acgcggcgcg agcagattca atccgaaggc gctgagcgca atcaacccgc
    14821 cggccgcgcc gtaggcggcg gttgcttccc gcgcgatcag ccaaaggagc gcgagcgcgg
    14881 cgacgaatga agggatgcgc cacgcgccct cccaccagcg cgtcagggcg aagagcgccg
    14941 ccgaaatcca accgaccagc ggcggcttcg tcgccacgct ctcgttaggc gtatgctggt
    15001 agagccagtg cccctgcccc accatctcga acgaggtgta ggcctgcttt gcctggtcgt
    15061 agtcgtcgag cgtccagggc acgttcgtgc tcgcaaaaaa caccatgatg agcgagagca
    15121 cgacccatcg cgtgcgcggc tgcgcgagcc attcgccttt cccgctcact cgtcaggcca
    15181 cgtcgcgcgc gtaccaatcc actttgcgcg tcacatacat caccaccgcg aggaggatga
    15241 aaagcgcgat cgcgcccatc aggagcgcgt aatcctgctg ccgcagagtg atgtagagaa
    15301 aagtgtaaac gcccgccagt ccggcgccga tcatcagggt gcgcaccccg ccaccgagga
    15361 agaaattgca gtaccaggtg atgagcacgg tcgcggcgac cgcggcgatc aagtaggcca
    15421 gaccgaagcc gataaactcg gagatcgaca aaaggagcag ataaaagagg cacagggccg
    15481 cgcccaccat cagatactgg aagggatgaa tcttctgccg cgccgtcact tcaaagagga
    15541 aaaatgtggt gaagacgagg acgaggaaaa gcacgccata cttgatggag cgttccacaa
    15601 atcgataggc gtccaggatg gacaaaaatt ccgcgccgaa aaacgaggcc gcaacggtct
    15661 tctcattaaa gcgctggttc ccgcggatgc tcgtccacga ttgcggataa tcgcggccgt
    15721 aataggacac tttccatttc gcggcaaagc catccggccg gaccgagcgc tccgcgggca
    15781 gaaacgcgcc gcggaatcca ggatcgggcc agttcgactt tagttcggcc tcgttgcgga
    15841 ctccgaacgg ggcgaagtag attccgccgc tcccattcac atcgagggaa atggcaaaat
    15901 ccgcggcgct cgcgagcggg tgatcgagcc cgagcaaagc ggtcgcgccg gtcgtgtaac
    15961 ccggcaactg cgcgcccggt tggagcgagc gtttcgtgcc gttccaatca aggacgagcc
    16021 cttcgcgcgt gccgcgcaaa tcactcacgg caatggtgac gaaggcgtct ttccattgca
    16081 catctttcag gtcgatcttg agagcgccga aatctggtgg cgcaaatttt cccgagagtt
    16141 tgacctgcgc gcgaaaaacc gccgcgttat aaatgccgcg gtgcaacgtc tgggtctgtg
    16201 catcgccggt gatctggagc gattcgggga gaaaaaatgc gttcgcgacc agcgtttcct
    16261 ccacttcgcg gcgttcgacg tgaccatccg gcatggtcgt atccttcatc gatctgaatt
    16321 tgtattggaa aggaacgccg agtaccgggc cgaggatgtt ttgttccttg ccccaggaat
    16381 ccgtgatctc gctgaccgct tcattccggc gctcgagccg gtcgctcagg acgccgtgaa
    16441 tcatggcgag cggaatgagc aggatgagaa tgagcgcgcc gacgccgaat aatttgatga
    16501 cggtgcggtt gcgtcgcagg aagggctgcg acctcggcaa tggcggcggc ggttgcattt
    16561 ccggcatggc gggagattga acggaggaaa cgcgaaaggc aaccaaagtc caccgcagat
    16621 gcgtggcgcg gcgccgactt ggaaagcgcg ccatcttccg cgatgctcgt cgctgtgaac
    16681 aaacaggaaa atccgtggat cgagatcgcg cccggaatca agcggcggac gcttgcggcc
    16741 ggcccctcga tgtatcagat gcgggccgag ctggcagccg gcagccgtct gcccgaacac
    16801 atgcatccgc aggaacagat tgcgcatctc atcaaaggtc ggatcaaact catcgtggcg
    16861 ggcgtcccgc acgaactggc ggcgggcgac gtcttttacc tcgggagcaa cctgccgcac
    16921 ggcgtggaga cgatcgaaga cacgacggtg atcgatactt tcagtccgcc gcgtgatgat
    16981 tatctggcgc tcgatgaaaa ggcgcggcgc gccggctgaa tgaagattat taccggcgga
    17041 caaacgggag tcgatcgcgc cgcgctcgat gccgcgatgt cgttaggcgt ggccgccggc
    17101 ggttggtgcc cggctggccg gctggccgag gacggcgtta tcccgccgca gtatccgctc
    17161 caggaattgc ccgggggtgg ctacccggaa cgcacggaac ggaacgtgcg cgaggcggat
    17221 ggcacggtca tttttcactc cggtctgctc gagggcggca cgaaagcgac ggccgatttt
    17281 tgtgcggaaa tgcagaagcc gtgctgctcg atcgacgcgc gcgcggcgac gccggaagag
    17341 acggcgcgcc agctcgcaga gttcgttagg gcaaatcaga tcagagtcct caatgtggcc
    17401 ggcccccgcg ccagccagtg gccgccggga tacgacttca ccgggcagac tctcgaccgg
    17461 ttgctccggc ccgcgccaaa actttcgttc gtggtcccgg cgcataacga agagcacgaa
    17521 ttgccggcaa gtctcaacgc cctgcgcgcg gccgccgatg cttcggggca aagctacgag
    17581 ctgattgtcg tcgacgatgc ctcaaccgat gcgaccgctg cgatcgcgca acaattcggc
    17641 gcccgggtca ttcccgtaaa tcgccgccag atcgccgccg tgcggaatgc cggagcgcgg
    17701 gaagcgcgcg gcgaaatcct tttcttcgtc gacgccgaca cgcggattgc accggatcac
    17761 gtcatcctgg gggtggccgc actcgccgag ggctgcgccg gcggcagcgc ccgagtcgtg
    17821 ccggacaggg agatgccgtt ttgggccagc gtttttctcc aggttttttc cgcggtctac
    17881 ttcaccgccg gacttggcgc gggcgcgttc atttttacgc ggcacgcatc gttcgaagcg
    17941 gccggcggtt tcgatgagca atattttgcg ggcgaagaag tctacctcag cctcgctttg
    18001 aaaaaacttg ggcagttccg gatcctgcgc gagccgatcg taacctcggc gcgcaaagtg
    18061 cgcatgcact cgccccggtt ttttctcacg cagacttttt ttctcctctt cggcggaaag
    18121 cgggccctgc gcagtcgcga ccgcctcggg ctttggtacg acggcaaacg cgaacggcac
    18181 gccaactgac gaaatgcagg ttgaacccac gggagctgaa gcttagagga gcgtggaggc
    18241 ctgacctatg gaaacaaaag taaaaccgtc aatcgatttg gcgaactgga tcgacgaaca
    18301 caaagacaag tttaagccgc cggtcagcaa ccagtatctc tacgacgggc gcgatttctt
    18361 cgtcatggtg atcaaggggc ccaatgcgcg gaacgacttt cacctcgtcg attcggagga
    18421 atatttttat cagctcaagg gcaacatcaa ggtccgcatc cgcgagggcg accagattgt
    18481 cgatcacctc gtgcgcgaag gcgaaacctt cttcattccg cctaacgttc cgcatgcgcc
    18541 cgttcgtccg ccggacacga tcggtgtggt ggtggagcgc cgtcgtccgc ctggcgagca
    18601 cgagcacgtc attttttatt gcgaaaaatg cggcgcactg gtcgaagata ttgacttcga
    18661 ttgcggcgac atcgtccagc attttagcca ggcgatgctc gatttctgga acgacgacgc
    18721 ccgccgcacc tgccggaagt gcggcacgaa ggtcgaacag ccgggaccga caaagccgtt
    18781 ttgatggata acggcgcgtt cgcagcagag gcggcgctgt cagccgccgc gtggcggtcg
    18841 ctggcgggat gcccgccgct acccg
//
LOCUS       DATMNS010000035         9285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4318523, whole genome shotgun sequence.
ACCESSION   DATMNS010000035 DATMNS010000000
VERSION     DATMNS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4318523"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1703)
                     /locus_tag="VNW28_01355"
     CDS             complement(<1..1703)
                     /locus_tag="VNW28_01355"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA08596.1"
                     /translation="MPYKMRLIVGLGFGFLAGFITSLMPLVLSSVASVVFHGATPSPS
                     TLATHPELMDKGPPINSLILLCLAIPAVMIARSLCGYVNTYYMTWVSAKVLTDIRTQL
                     FGKMLSHSMDFFNKMHSGFLMSRITNDTQMMQAALSSVSSDLFKQPVAIIGGVSVLLY
                     MDWKFTLVTLVLFPSCLVPLRMYGKRARKAVMGQQEELGQMVVTMQETFAGIRVVKSF
                     AREEYQEKSFRRSNRIQFDNSMRITRSMEAVSPLVESIAAIGVGFALLYVYFAHLPAA
                     KFMALIAGIFLLYDPIKTLSRIHITMQRSIQATVEIFRILDSDSSVQDKPDALTLTSS
                     KGALELEHINFRYVGGIDDALKDFSLKIEPGKSYALVGASGAGKSTILSLLLRLYDPT
                     VGVLRIDGRDLRAVTQKSLRQQIGLVSQETFLFHDTIFQNILFGRLDATKEEIYDAAK
                     TAYAHEFIMAQPKGYETIIGDKGCLLSGGQQQRLAIARALLKNAPVLLLDEATSALDS
                     ESEQQIQLALERLAQGRTVIAIAHRLSTILSADQIVVMEKGRIKEIGTHAELLENSSY
                     YR"
     gene            complement(1793..2809)
                     /gene="rfaQ"
                     /locus_tag="VNW28_01360"
     CDS             complement(1793..2809)
                     /gene="rfaQ"
                     /locus_tag="VNW28_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipopolysaccharide heptosyltransferase
                     III"
                     /protein_id="HXA08597.1"
                     /translation="MNVLLIQLKRIGDLVLTVPAIAALRKSFPAAKISLIVAHGSREL
                     LPAIPGVDHTFVARGRVTDAPQWFALATSTFDYCLDFSRTDRSAFLTALSGARNRITY
                     DTIRREPLRQLSYNEFVPSQVRFAHTVDHHLALLAPLGVHDASPEIRLALPPEAKARA
                     AAIMNAHHLAGDFVILHPGSARAEKFWLARRWAEVANRITAHEQMPVVLTGGKARMEQ
                     EHIARIKKHLDQPVVDLSGHLDLLSLAVLLQQARLLLTIDSAPMHLAAALGTPQVALF
                     GPTNPFHWRPRSTPAIVLNAGNDHPATDFVPIQPPVAMKEISTKQVIDAMQTLLSIPA
                     APVA"
     gene            complement(2979..4154)
                     /locus_tag="VNW28_01365"
     CDS             complement(2979..4154)
                     /locus_tag="VNW28_01365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXA08598.1"
                     /translation="MATPRRILPNHLLYSIFARIGGSGLDTDAFETLRASYHGGFLGK
                     AVAYDNRQTEIPAARIDSLRWHPVRLLSFLDSPYYYGAKKKYLDWITARHLTTGRYDL
                     FHSWSGDCLLSLREARKRGIPSVLEIPTWHRDRGKVKRGAAPPPPEPELPWRKRWKED
                     LLLQRDRFVEEYDLADLILVLSERAAETFRVQGFPEQKLFYLPRGVNVDRFTPGARPA
                     HFRAVFSGALIERKGVHHLLEAWHRLKLKNAELWLVGSVHAEIRPYLEKYADESVKVI
                     GFAREPEKYLRQSTIHVFPSQCEGSAKVTYEAAACGLPQITTREAGDVVEDGLQGIIV
                     PPADVDTLVAAIQQLYDHPEIVEQMSIAARRRVVENFTWDHFRARLLDAYEVAMRME"
     gene            4249..5388
                     /locus_tag="VNW28_01370"
     CDS             4249..5388
                     /locus_tag="VNW28_01370"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXA08599.1"
                     /translation="MLPAMSGPRLTIGLVRRGHSETGGAEAYLKRLARGLSESGHATR
                     LFTDAAWPAREWPWGPMTRLRGESARRFADELEKIGPEDHCDLLLSLERVWRCDVYRA
                     GDGIHQAWMRRRAAVSSFARRLLSRLNAKHRETLELEKALFTGGAGRVIANSQMVKRE
                     AEEFYGYPAGQIEVVPNGVPLVEFQPDPEARRRRRKILRLRDDEIALLFVGSGWERKG
                     LRFALDAVKSLGTNYRLLIAGRGRRRLASGSSVQWLGAVRDLPALYSAADIFLLPTLY
                     DPFSNACLEALAAGLPVVTTRANGFSEIIEHGVHGSIVERAPDTAEIRAALLFWSDPE
                     RRRAARSAILERAGHFDISVNVERTLAVLHLARNADAESGKIRKT"
     gene            complement(5337..5654)
                     /locus_tag="VNW28_01375"
     CDS             complement(5337..5654)
                     /locus_tag="VNW28_01375"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXA08600.1"
                     /translation="MPASQPNVLPLEGEIDLHVSPQVSASLAALIAKKPLQVVVDLSK
                     VNYIDSSGLAVLIEAMQNVGGYGGKFALSGLQEGVRPIFEIARLDQVFRIFPDSASAL
                     RAK"
     gene            complement(5780..6484)
                     /locus_tag="VNW28_01380"
     CDS             complement(5780..6484)
                     /locus_tag="VNW28_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXA08601.1"
                     /translation="MFNLKSSIPKRLLLFDIDGTLIDSGGAGIQSLKDVLQQQFGVTD
                     DLRGVEIAGQTDTGIVHQILRKQNIAVNEEMTAAFLDLYVDFLARELPLRKGKLLPGV
                     KELLPRLQARPQNVLALLTGNLRRGAELKLAHYGIWDFFEFGAFADDHYDRNELGPFA
                     QRRAREKHAIDFTAAEIDVIGDTPHDIACGKAIGARTIAVATGNFSRAQLATHQPDRI
                     VDDFSKLDAVMADLGW"
     gene            6491..7336
                     /gene="gluQRS"
                     /locus_tag="VNW28_01385"
     CDS             6491..7336
                     /gene="gluQRS"
                     /locus_tag="VNW28_01385"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HXA08602.1"
                     /translation="MKSGSGKPKSEIPYRGRLAPSPTGYLHAGHALTFWRAQERCRER
                     DGALVLRVEDLDRARCRPEFREAIFEDLRWFGLQWAEGPFLQSERRLFYLEAWKKLRD
                     HGLVYPCNCSRRDVTSAAGAPHAEEEEPLYPGTCRPSCLTIPVADDPAGVNWRFRVPE
                     NDALQFFDQCAGRQRVIAGRDFGDFIIWRKDDVPAYQLAVVVDDAAMRITEVVRGADL
                     LLSTFRQLLLYRALDLPAPDFCHLPLVTDSSGQRLAKRHNALSLRTLRAEGVTPDEIR
                     RRFAL"
     gene            complement(7399..7902)
                     /locus_tag="VNW28_01390"
     CDS             complement(7399..7902)
                     /locus_tag="VNW28_01390"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXA08603.1"
                     /translation="MPKLSILLEHARALHELTENQITIGHGPDNMIQIDDPSVSSRHA
                     RLLLTDGRYQLEDLGSTNGTRVNSELVTDTFLRIGDRIRFGKVEARYESDATGEAQPL
                     TDADEIEPRPAETSARPADFANASPFPYRQKEKDTVATAILAAAALAILAFLLSMLGL
                     FQIHGPL"
     gene            complement(8006..>9285)
                     /locus_tag="VNW28_01395"
     CDS             complement(8006..>9285)
                     /locus_tag="VNW28_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXA08604.1"
                     /translation="VVMVGGTAQIWHDVVDTPLGPGRLGPKIHLEVMAAALAHEFLWM
                     TPLAVDFLLVIAAGALAWALIAFVQRPLTVLFLLAGTTIAYFAAARIAFDYRGLLLIT
                     VPVLTVFVLSGAFSLGYEYALERIEKLRTRRTLERYVSRNLVEEILDNPGGFYSSLKG
                     VRIPATILFSDIVGFTSLTENADPEALVKQLNEYLSQMTKAVFDHGGTLDKFIGDAVM
                     AVWGNVRSHGKAEDTKMAARAALAMRRELWTLNAGWHARGIAPFAIGIGINQGDVLGG
                     NIGSQEKADPTVIGDAVNLASRLEGLTRIYGLDILLGPTATELVRDEFHVRSVARVQV
                     KGKNEPVEITTLIAARSAGIDPDFLRHLEGYEEAFKKFRLREFGEAKILLTRFLEAYP
                     KDFLAKMYLERALEYEQTPPDEEWNAVEVFRRK"
BASE COUNT         1813 a         2773 c         2902 g         1797 t
ORIGIN      
        1 cgatagtagc tggaattctc gagcaactcc gcgtgcgtgc cgatttcttt gatccggcct
       61 ttctccatga ccacgatctg gtcggcagac aaaatggtgg aaagccggtg cgcgatggcg
      121 atcacggtgc gcccttgcgc aaggcgttcg agggcgagtt ggatctgctg ttcggactcg
      181 gagtcaagcg cagaggtggc ttcgtcgagg agcaggaccg gggcattctt gagcagggcg
      241 cgggcgatgg caagccgctg ttgttgtccg ccggagagca gacatccctt atcgccgatg
      301 atagtctcgt agcctttcgg ctgggccatg atgaactcgt gcgcgtaagc ggttttggcg
      361 gcgtcataaa tctcctcctt cgtggcatcg agtcggccga acaggatatt ttggaatatg
      421 gtgtcgtgga aaaggaacgt ttcctgtgaa acgagcccga tttgttggcg cagcgatttc
      481 tgcgtgaccg cgcgcagatc gcggccatcg atacgcaaga cgccaacggt tgggtcgtaa
      541 aggcgcaaga ggagggaaag gatggtgctt ttccccgcgc cactcgctcc aacgagggcg
      601 taactcttgc cgggttcgat cttgaggctg aagtccttca acgcatcatc aatgccgccg
      661 acgtagcgga agttgatgtg ctccaactcg agcgcaccct tcgaggaggt aagtgtgagc
      721 gcgtcgggtt tgtcctgcac gctcgagtcg gaatcaagga tgcggaaaat ctccaccgtc
      781 gcctggatgg agcgctgcat cgtgatgtgg atgcggctaa gcgtcttgat cggatcgtag
      841 aggaggaaaa tgcccgcgat gagagccatg aatttcgcgg ccgggaggtg cgcaaagtag
      901 acgtagagaa gggcgaagcc gaccccaatt gcggcgatcg actcgacgag gggcgaaacg
      961 gcctccatcg agcgcgtaat gcgcatcgaa ttgtcgaact ggattcggtt actgcgccgg
     1021 aacgattttt cctggtactc ctcgcgcgca aacgacttca ccacccgaat gccggcgaag
     1081 gtttcctgca tcgtcacgac catttggccg agttcttcct gctgacccat gaccgccttg
     1141 cgggcgcgct tcccatacat cctgagcggc acgaggcagc tcgggaagag cacaagggtc
     1201 acgagcgtga acttccaatc catgtagagc agcacgctga cgccgccgat gatcgcgacc
     1261 ggctgcttaa acaagtcgct gctgacgctg gagagcgccg cctgcatcat ctgggtgtcg
     1321 ttggtgatgc gggacatgag aaagcccgag tgcatcttgt taaagaagtc catcgagtgg
     1381 ctcagcatct tgccaaagag ctgggtgcgg atgtcggtca gcactttcgc gctcacccaa
     1441 gtcatgtagt aggtgttaac gtagccgcag agactgcggg cgatcatcac ggcaggaata
     1501 gcgaggcaaa gcaggatgag ggaattaatc ggcggccctt tgtccatcaa ttccggatga
     1561 gtcgcgagag ttgacggcga gggtgtggcc ccgtggaaaa cgacgctggc cacactggac
     1621 aggactagcg gcatgagtga ggtgataaag ccagcaagga agccaaaacc caggccaacg
     1681 atgagccgca ttttatacgg catggcatag gcaaaaagtc gtcggtaagg accccacccg
     1741 atgcggatgg tggtccagaa agactgcttc ggctttcgga tctgcggctc gctcatgcaa
     1801 ctggcgctgc cggaatcgac agcagcgtct gcatagcatc gatcacctgt ttcgtcgaga
     1861 tttccttcat cgcgaccgga ggttggatcg ggacaaaatc ggtcgccgga tggtcgtttc
     1921 cggcgttcaa aacgatcgcc ggcgtcgagc gcggccgcca gtggaacgga ttggtcggcc
     1981 cgaaaagggc aacctggggc gtgccgagcg cggcagcgag gtgcatcggc gcggaatcga
     2041 tcgtgaggag caaccgcgcc tgctgcaaca agaccgcgag ggagaggaga tcaagatgtc
     2101 cggagagatc gaccaccggt tggtcgaggt gcttcttgat tcgcgcgatg tgctcctgct
     2161 ccatccgcgc ctttccgccg gtgagcacga ccggcatctg ctcgtgtgcg gtgatccggt
     2221 tcgccacttc cgcccagcgc cgcgccagcc agaatttttc ggcgcgcgct gatcccgggt
     2281 ggagaatgac aaaatccccg gcaaggtggt gcgcgttcat gatggcagcg gcgcgagcct
     2341 ttgcctctgg cggcagggcg agtcgaatct ccggcgaagc gtcgtgcacc ccaagcggcg
     2401 cgagcaacgc gaggtggtga tcgaccgtgt gcgcgaatcg gacctgcgac ggaacgaatt
     2461 cgttataact cagttgccgg agcggctcgc gtcggatcgt gtcgtaggtg atccggttcc
     2521 gcgccccgga gagcgcggtg agaaaagctg agcggtccgt gcgcgaaaag tcgaggcagt
     2581 agtcgaaggt ggaggtcgcg agcgcgaacc attggggcgc gtcggtcacg cgcccgcggg
     2641 ccacgaaagt atgatctacc ccgggaatgg ccggcagcag ctcgcgcgag ccgtgcgcaa
     2701 cgatgagcga gattttggcc gccggaaaac tcttccgcag cgcggcgatc gcggggacgg
     2761 tcagaaccag atcaccgatc cgtttgagct ggatgaggag aacgttcaaa cactctccta
     2821 acgaggctgt gcctcagagg ggaagtgacg agtgacgaaa gaacggatgg aatgacgaat
     2881 tccgcacggc gaaggacgaa tcaatgacca agcacgaagt ccaaatgatg ggtcgccaga
     2941 cgacgcgaat tgctcattcg accttgggaa ttggttcgtc attccattcg cattgccacc
     3001 tcgtaagcgt ccagcaggcg cgcgcggaaa tggtcccagg tgaaattttc caccacccgc
     3061 cggcgagcgg cgatgctcat ctgctccacg atttccggat gatcgtagag ctgctgaatg
     3121 gcggccacga gcgtatcgac gtcggccggc ggaacgatga ttccctgcaa gccatcctcc
     3181 accacatcgc ccgcttcgcg cgtcgtgatt tgtggcaacc cgcaagccgc cgcctcgtag
     3241 gtgacttttg cgctgccttc gcattgcgaa gggaaaacgt gaatcgtcga ctggcgcaga
     3301 tatttttccg gctcgcgggc gaaaccgatc accttgacgc tctcgtccgc atacttttcg
     3361 agatacggcc tgatctcggc gtgcaccgag ccgacgagcc agagttccgc attcttgagc
     3421 ttgaggcgat gccaggcttc gagcaaatgg tgcacccctt tgcgctcgat cagcgcgccg
     3481 gaaaaaaccg cacggaaatg cgccggccgc gcgccgggcg taaagcggtc gacattcacc
     3541 ccgcgtggca ggtaaaaaag tttctgctcg ggaaagccct ggacgcgaaa agtctcggcc
     3601 gcccgctccg agagcacgag gatcaaatcc gcgagatcgt attcttcgac aaatcgatcg
     3661 cgctggagga gcagatcttc cttccaccgt tttcgccagg gcaattctgg ctccggcggc
     3721 ggaggggcag ctccccgttt taccttgccg cggtcgcgat gccaggtcgg gatctcaagg
     3781 accgatggga ttccgcgttt gcgtgcttcg cggagcgaga gtagacagtc gcccgaccag
     3841 ctgtgaaaca aatcgtagcg gccggtggtg agatgccgcg ctgtgatcca gtcgagatat
     3901 ttctttttcg cgccgtaata atatgggctg tcgaggaacg aaaggagccg caccgggtgc
     3961 cagcgcagcg aatcgatccg ggccgccgga atttccgtct gccggttatc gtaggcgacg
     4021 gctttgccga gaaacccgcc atgataggag gcgcgcaacg tttcgaacgc atccgtatcc
     4081 aggcccgagc cgccgatgcg cgcaaagatc gagtaaagca gatggttcgg caaaatgcgc
     4141 cgtggcgttg ccatcgagat tgcgcgcggc ggggctttta gagtttcacc caccatgacc
     4201 ctaacattat tgttgggaat gcgggcaagt aaagggtaga actcccgcgt gctgccggcc
     4261 atgagcggtc cccgcctaac gatcggtctg gtgcgacgcg gtcattccga gaccggcggc
     4321 gccgaagcct acttgaagcg cctcgctcgc ggcctgagcg aatccggcca cgccacccgg
     4381 ctctttaccg atgcggcctg gccggcgcgc gaatggccct gggggccgat gacgcgactc
     4441 cggggagaga gcgcacggcg cttcgccgat gaactggaaa agatcggacc ggaggatcat
     4501 tgcgacctcc tgctcagtct cgagcgggtc tggcgctgcg atgtttaccg cgcgggcgac
     4561 ggcattcacc aggcttggat gcgccggcgc gccgccgtct cctcattcgc ccgcagattg
     4621 ctcagtcgtc tcaacgccaa gcaccgcgaa acgctcgaac tcgagaaggc gctcttcacg
     4681 ggcggcgccg gccgcgtcat cgccaactcc caaatggtga aacgtgaggc ggaggaattc
     4741 tacgggtatc cggctggcca gatcgaggtc gtgccaaacg gtgtcccgct ggtggagttc
     4801 cagccggatc ccgaggcgcg ccggcggcga cgcaaaatcc tgcgcctccg ggatgacgag
     4861 atcgcgctcc tttttgtcgg ctccggttgg gaacgcaaag gcctgcgctt cgcgctcgac
     4921 gccgtcaaat cgttaggcac aaactaccgc ctcctgatcg ccggccgcgg ccggcggcgg
     4981 ctggcaagtg ggagttccgt ccaatggctc ggggccgtgc gcgatctgcc cgcgctctac
     5041 tcggccgccg acatcttcct cttgccgacc ctctacgatc cgttttcgaa tgcctgcctc
     5101 gaggcgctcg ccgccggtct gcccgttgtc actacgcgcg ccaacggctt cagtgagatc
     5161 atcgagcatg gtgtccatgg gtcgatcgtc gagcgcgccc cggatacggc ggaaattcgc
     5221 gccgccctcc ttttctggtc ggatcccgag cgccggcgcg ccgcgcgctc agcgattctc
     5281 gagcgcgccg gtcactttga tatctcggtc aacgtcgagc gcacgctcgc cgtccttcat
     5341 ttggcccgca acgccgatgc ggaatcgggg aaaatacgga agacctgatc gagccgcgcg
     5401 atttcgaaaa tcggccggac accctcctgc aagccactga gggcgaattt gccgccgtaa
     5461 ccgccgacgt tttgcatcgc ctcgatcagc accgcgagac cggagctgtc gatgtagttg
     5521 actttggaga gatcgacgac cacttgcagc ggcttcttcg cgatcagcgc agcgagcgac
     5581 gcgctaacct gcggcgacac atgaaggtca atttcgcctt cgaggggaag aacgttaggt
     5641 tggctggcag gcatgacgcg tagcaaacca tgtcactctt gcccgttccg tcaatcgcgg
     5701 cactcgttgc ggttcaactc gtgctcgtgt catgaagcaa aaggggatcg atcacgagca
     5761 ggagtaagac atggattcct caccagccga gatcggccat gaccgcatcc aacttggaga
     5821 aatcgtccac gattcggtcc ggttggtgag tggccagttg cgcgcgcgaa aaatttccgg
     5881 tcgccacggc gatcgtccgc gcgccgatcg ccttgccgca cgcgatatcg tgtggtgtat
     5941 ccccaatcac atcgatctcg gccgcggtga aatcgatcgc gtgcttttcg cgcgcgcgcc
     6001 tttgggcgaa aggtccgagc tcgttgcgat cgtaatgatc gtcggcgaag gcgccgaatt
     6061 cgaaaaaatc ccaaataccg tagtgcgcaa gtttcagctc cgcgccgcgc cgcagattac
     6121 cagtgagcag ggcgaggacg ttctgcggcc gcgcttgcaa gcgcgggagc aactccttca
     6181 cgcccgggag aagttttccc ttccgcagtg gcagttcccg cgcgaggaag tcgacataga
     6241 gatcgaggaa cgccgcggtc atttcctcgt tcacggcaat attttgcttc cgcaaaattt
     6301 ggtggacgat gccggtgtcg gtctggcccg cgatctccac tccgcgcagg tcgtcggtca
     6361 cgccaaattg ttgctggagc acgtccttga gcgattggat gccggcgccc ccggaatcaa
     6421 tcagggtgcc gtcgatgtcg aagaggagaa ggcgctttgg aattgatgat ttcagattga
     6481 agatttctga ttgaaaagcg gatcgggcaa accgaaatct gaaattccct atcgcggccg
     6541 tctcgcgccg tcgccgaccg gttacctgca tgccggccac gcgctcacct tctggcgcgc
     6601 gcaggagcgc tgccgcgaac gcgatggcgc gctcgtcctg cgcgtggaag atctcgatcg
     6661 cgcgcgctgc cggccggaat tccgggaggc gattttcgaa gatctgcgct ggtttggttt
     6721 gcagtgggcg gagggcccgt ttctgcaaag cgaacgccgc ctgttctacc ttgaagcttg
     6781 gaaaaaattg cgcgaccacg gtctggtgta tccctgcaac tgctcgcgcc gcgacgtgac
     6841 gagcgcggcc ggcgcgccgc acgccgagga ggaggaaccg ctttacccgg gcacctgccg
     6901 gccctcctgc ctaacgattc ccgtggcgga tgatccggcg ggggtgaact ggcgcttccg
     6961 cgttcccgag aacgatgcgc tccaattttt cgaccaatgc gcgggccgac agcgcgtgat
     7021 cgcgggccgc gacttcggcg atttcattat atggcggaag gacgacgttc ctgcctacca
     7081 gctcgccgtg gtggtcgatg acgccgccat gcggatcacg gaggtggtgc gcggtgccga
     7141 cctgctcctc tccactttcc ggcaactact cctttatcgc gcgctcgatt tgcccgcgcc
     7201 ggatttttgt cacctgccgc tcgtgaccga ttcgtcaggc caacgcctcg cgaaacgcca
     7261 caacgccctc agtctgcgca cgttgcgggc agagggagta acacctgacg aaattcggcg
     7321 gcggttcgca ttgtagggcg cgccatcctg gccgccccgc ggtggatacc caaacggggg
     7381 cggccgggat ggcgcgccct acaacggccc gtgaatctgg aacagaccga gcatgctcaa
     7441 gagaaacgcg aggatggcga gcgccgcagc cgccaggatt gcggtcgcaa cggtgtcctt
     7501 ttccttctga cggtacggaa agggcgaagc gttggcaaag tccgccggcc ttgcgcttgt
     7561 ctcggccggc cgcggttcga tttcgtccgc atcggttaaa ggttgcgctt caccggtcgc
     7621 atccgattcg tagcgcgctt ccacttttcc gaatcggatc cggtcaccga tccggagaaa
     7681 ggtgtcggtc accagttcgc tgttcacgcg ggtgccgttg gtcgagccga gatcctcgag
     7741 ctgataacgg ccatcggtga gaaggagccg agcatgccgg ctggagaccg acgggtcatc
     7801 gatctggatc atgttgtcgg gcccatgccc aatcgtgatc tggttctcgg tcaactcgtg
     7861 cagcgcgcgc gcgtgttcca ggagaatgga aagcttcggc atgccgcagc agtatcagga
     7921 gatccagtct ccagcaacgg caatagcagg cttcttcgag cttcgtcatt ggatcattgt
     7981 ttcgagcttc aacattcgga tttcgtcatt tcctcctaaa cacttcgacc gcattccact
     8041 cttcatccgg cggcgtctgc tcgtactcga gcgctcgctc cagatacatt ttggccagga
     8101 aatccttcgg gtaggcctca aggaaccggg tgaggagaat tttcgcttcg ccaaattcac
     8161 gcagccgaaa tttcttaaac gcctcctcgt agccctcgag atgtcgcaga aaatcggggt
     8221 caatccctgc actccgcgcg gcgatcaggg tcgtgatttc caccggctcg ttttttcctt
     8281 tcacctgcac tcgcgccacc gagcgaacat ggaattcgtc gcgcaccagc tcggtggcgg
     8341 tcggcccgag caggatatcc agcccgtaaa ttctcgtcag gccttcgagg cgtgaagcga
     8401 gattgaccgc gtcgccgatc accgtcgggt cggctttttc ctgcgagccg atgttgccgc
     8461 ccagcacatc gccctggttg atcccgatgc cgatggcaaa cggcgcaatc ccgcgcgcat
     8521 gccagccggc attcagggtc cacaactcgc gccgcatggc cagcgccgcg cgcgccgcca
     8581 tcttcgtatc ctccgctttg ccatgactgc gcacattgcc ccagaccgcc atgaccgcgt
     8641 cgccgatgaa tttatcgagt gtccccccat ggtcgaaaac ggctttcgtc atttggctca
     8701 ggtattcgtt gagttgtttg acgagcgctt ccggatcggc gttttccgtc aggctggtga
     8761 agccgacgat gtcggaaaac aaaatcgtgg ccgggatacg gacacctttg aggctgctat
     8821 aaaagccgcc ggggttatcg agaatttctt cgaccaggtt tcttgagaca taacgctcga
     8881 gagtccggcg agtgcgcagt ttctcgatcc gctccagcgc gtattcgtag cctaacgaaa
     8941 aggcgccgct cagcacgaaa accgtcaaga ccggcaccgt aatcaggagc aggccgcgat
     9001 aatcgaacgc gatccgggcc gccgcgaaat aagcgatcgt ggttccggcg aggaggaaca
     9061 agacggtcag cggacgctgc acgaatgcga tcagcgccca ggcgagcgcg ccggccgcga
     9121 tcaccaaaag aaaatcgact gccagcggtg tcatccagag gaactcgtgc gcgagggccg
     9181 cggccatcac ttcgagatga atcttgggtc ccagccggcc cggaccgagg ggtgtatcga
     9241 cgacgtcgtg ccagatctgg gccgtcccgc ccaccatcac gacct
//
LOCUS       DATMNS010000036         9820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_760500, whole genome shotgun sequence.
ACCESSION   DATMNS010000036 DATMNS010000000
VERSION     DATMNS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760500"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..1439)
                     /locus_tag="VNW28_01400"
     CDS             complement(102..1439)
                     /locus_tag="VNW28_01400"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXA08605.1"
                     /translation="MKRLFFIWLALAFVSLPAARGLTLDAALARSLEKNPRIQQAKTA
                     VEQAAGQRLVFRSIALPDVAVGILGGVQGGHRAGQAPTEPFGFAQGTVRQALFQVAIP
                     ASLRRGDIEVLLAAQQLNVAVVEQLHAGRTAFYTALYERSLEELGRSQRSRLDANLTS
                     QEARYEAGDVDRSALASATLLARELDPQIEDAHRSYGAAILQLATAIGADLAPGAALP
                     SPEGALDFRPVNFPLASETSAALERRADLRLARLLVRAGREDQRISAAAYYPRLDLEL
                     FGRYIPVTIQQASSGSAQRTDDLVSSEIRAGPSYTWRVIDNGKVGWAVLRERSIREMN
                     ELQLKKLEASVLRELAVLQDNLRAIAARHDSLSKAVAVAEKNVAVVENSWEQGLTSQL
                     DFRTAEAGLLATRSGILKAAYEQEMALAEWDRATGRYFQFSDETTANVHSSNQ"
     gene            complement(1436..2836)
                     /locus_tag="VNW28_01405"
     CDS             complement(1436..2836)
                     /locus_tag="VNW28_01405"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXA08606.1"
                     /translation="MRSVFRLALLALVALPSIAPVRGAPRYTIDDVVRLAQDQNPEIA
                     IARKKIQAAHGGQVEARSGYLPSVVSSGLYRRREDAESTRLRPDDYSTSVRVVQNLYT
                     GGATSNQVAIAQLNLAKQQDEFETVTDRVTMDVRLAFYELLLNREKILVREQSVGVLR
                     EELKSQRERLSAGTVGPLDASRAEVALANEEPELVQAQTDLRNSYLHLSDLCGIDARS
                     SGEPQFEAVGTLQYEPRHPDLDATLARALAERPEIRSAQKDVAIEEKQLEIDRSATRP
                     HVEFFTGYEVYSERDPLVGPEFNHGYVVGLNGSWALFDGYATRGRMEATRARRDGALL
                     ALKAVKLGIESEVRSAFLDLQQADSVLQAQSKNVQTADDALTFAKSNFAAGLGTQLDV
                     LQAAADVTRTRTTRLSAIYLHNAALARLDRASSRDSATLAFEVRSTKADQPKGAAAGE
                     QVFQFARPPSSLGQGK"
     gene            3084..4187
                     /locus_tag="VNW28_01410"
     CDS             3084..4187
                     /locus_tag="VNW28_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HXA08607.1"
                     /translation="MSKIPVAIIGVGNCASSLVQGISFYGGTPRNGASVGLMHQQVGP
                     YWPDDIEVVAAFDVDRRKVGIDVSEAIFAPPNCTKIFCEKIAKTGTIVKMGCVLDGVG
                     AHMSGYDPLRTFQPLEKESTRERIIAELEESGAEIMVNYLPVGSEEATRFYVGCALEA
                     GLALVNNIPVFIASDPVWAKRFEEKNLPLVGDDIKAQMGATVLHRTIVDLFRKRGVKI
                     ERTYQLNTGGNTDFLNMLNRGRLESKKTSKTEAVQSVIGARLADENIHIGPSDYVPWQ
                     NDNKICFIRVEGKLFGDVPMEIDLKLSVEDSPNSAGVAIDAIRCCKLALDRGKGGVLH
                     SASAYFSKHPAIQLTDDEAYQHVEEFIRGERDS"
     gene            4285..5007
                     /locus_tag="VNW28_01415"
     CDS             4285..5007
                     /locus_tag="VNW28_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA08608.1"
                     /translation="MSEEIWLSCRAIERYLGEDDSRVHALRGVSLDLEPGSVHAVVGP
                     SGCGKSTLLYILGLLDYPDAGTLTIDGHLMAQLPDDEVARKRNELLGFIFQFHFLLED
                     FTAQENVMIPMRRLGRLTEEAMLERAARLLEEVDLGGKLRRPSRHLSGGEQQRVAIAR
                     ALANDPHVILADEPTGNLDTKNSRRAFRLLQKIVREERKALLLVTHNPEIAEACDWIH
                     EMQDGVIVGSHPRGQRTAFSDA"
     gene            complement(5107..6615)
                     /locus_tag="VNW28_01420"
     CDS             complement(5107..6615)
                     /locus_tag="VNW28_01420"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXA08609.1"
                     /translation="MDKDSSSHGATLARGAAINTLAFLASNLRGIFTFLVARLLGSAV
                     LGIFGLAWATMDLVSKFCTLGFDYSAIAFIAQAKGTGDRESSRRVRRAALIVSCTSSV
                     LLAVGGFFLLWTFGPAAGLRPEFARASAVMLLALPGVTLYRVNTAFSRGMTVMQHDIY
                     SRGLTESFGTAAALLVAFALGARQLAPEFAGIAGTLASGLVAFFLARRLFTAQGAGAV
                     LPNDDLVPSLLRTSAPIALYDLLNIGIMRIDLIMLGWFVGRAPGVTLQTLGIYAAAVE
                     VGGGLRKVSQSFTPIFTPIVARQVGGRQIREAEASYGYLARWMLAILLPAVVVLALAG
                     GAIMAIFGPTFYRGGLWAALIGCGCALNAFVSLGETILMVERPKINLMNSTIAFVAAV
                     GLNLVLIPAYGALGAAFGVLCPYMIKGLLRWAEIRYYYEWRWPWHALFKPWIAALLAL
                     PCALLLRWVSPAWWSDLAAAALYLAGYFIAWRIIGLEPNDRAVLDQLFKRKS"
     gene            complement(6621..7772)
                     /locus_tag="VNW28_01425"
     CDS             complement(6621..7772)
                     /locus_tag="VNW28_01425"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXA08610.1"
                     /translation="MESSSPSRALRIAIDARYVREKPSGIGAYVQALVERLPSLAASD
                     HFFFWAHPLAPRPLSPAPNTSATIVKAGPNSPWPVLWPEHYASFAEVDVFHSPHNLMP
                     RGLPCASVITVHDVMALEEPAMHLQGLERVVKSVYYRQAVWRALREATRLVAQSRATA
                     DRICALLPEAAKRLTVIWSAPDRDLGPPNDPAAAQERAARLTKSEAPYLLLVGANTPS
                     KRHDLALAAFGRAVPRPWLLVVVQRRKASKGLVRLAKQLQVDDRVVWLETVARPDLVT
                     LLQAAGGLIQPSVYEGFGLPVLEAMACGCPVVASDIPPLREVTAGTALLFKAGDVTGL
                     ANALRDFVQSPGLRESLAGQGLARSRDFSWDRCAKETLAVYHDAVATNR"
     gene            7864..8655
                     /locus_tag="VNW28_01430"
     CDS             7864..8655
                     /locus_tag="VNW28_01430"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXA08611.1"
                     /translation="MKKIFLTGASSGIGLAIAQKLCAAGHEVWGTSRDPARIPSLGKL
                     HPVHLDLAELEGIRGAFENSWREAGYFDVLINNAGGGHFDAAEILGPERIEELFRTLV
                     FAHIELCRLALGAMNARQSGLIINVTSLASRLPVPYMSAYNAAKAAMASYTFSLQLEP
                     RNRRVRIVDLQPADIRTSFNDSISPQPPGDRHVAKTWEVAQRNMRNAPPPDLVACRVL
                     RLLASANPPPRLTVGGVFQSRIAPLIFRFLPQRVRVWGLKNYYGI"
     gene            complement(8841..9215)
                     /locus_tag="VNW28_01435"
     CDS             complement(8841..9215)
                     /locus_tag="VNW28_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXA08612.1"
                     /translation="MAIDRHWAASAAGDQVAEHDIYHDDIVCEYPQSGEIIRGRSNLQ
                     ALRSHHPGRPSGFSIRRILGKSNLWITEYVIIYEGKRVYTVSIMEFRDGKVSHETQYF
                     ADPFNAPGWRAQWVETVAADNS"
BASE COUNT         1958 a         3045 c         3042 g         1775 t
ORIGIN      
        1 ctccacttcg gcgaccggca gcgaccaaag aaaaatcgcg aggcagacga gcaaggcgac
       61 cggcaggatc gtccagagcg ttcggtttga aatgggcagg ttcactggtt ggaactatgc
      121 acgttggcgg tggtctcgtc cgagaattga aagtagcggc cggtcgcgcg atcccattcc
      181 gcgagcgcca tctcttgctc gtaggcggct ttcaggatgc cactgcgagt cgcgagcaag
      241 cccgcttccg cggtgcgaaa gtccaactgc gaagtgaggc cctgctccca gctgttctcg
      301 acgaccgcaa catttttctc cgccactgcg actgccttgc tcagcgaatc atggcgcgcg
      361 gcgatcgccc gcagattatc ctgcaggacc gccaattccc gcaggacact cgcttcgagt
      421 ttctttaatt gcagttcgtt catttcgcgg atggatcgct cccgcaggac cgcccagccg
      481 acctttccgt tatcgattac ccgccaggtg taactcgggc cggcccggat ctccgacgaa
      541 acaagatcgt cggtccgttg cgcggaaccg gagctggcct gttggatagt cacgggaatg
      601 tagcggccga aaagctcgag atcgagtcgc ggataatatg cggccgcgct gattcgctga
      661 tcttcgcgcc cggcccgcac cagcaggcgc gccaggcgca gatcagcgcg acgctcgagc
      721 gcagcgctgg tctcgctcgc gaggggaaaa ttcaccgggc ggaaatccaa cgctccctcg
      781 ggagaaggca aggccgcgcc cggcgcgaga tcggcgccta tcgccgtcgc cagttgcaga
      841 atcgccgcgc cgtaggagcg gtgtgcgtcc tcgatttggg gatcgagctc ccgcgcgagg
      901 agtgtcgcgc tggcgagcgc gctgcgatcc acgtcgcccg cctcatagcg cgcctcctgg
      961 ctcgttaggt tggcatcgag gcgcgagcgt tgcgaccggc cgagttcttc gagcgagcgt
     1021 tcataaagcg cggtgtaaaa tgcggtgcgc ccggcgtgca gttgttcgac gaccgcgaca
     1081 ttcaattgct gggcggcgag cagcacctcg atgtcgccgc ggcgcaagga agctggaatt
     1141 gccacttgga aaagcgcctg gcgcacggtc ccctgcgcga aaccgaacgg ctcggtcggt
     1201 gcctgaccgg cacgatggcc gccctgcact cccccgagaa tccccacggc aacgtcgggc
     1261 agcgcgatgg agcgaaagac gaggcgctgg ccggcggctt gttcgactgc ggtctttgcc
     1321 tgctggatgc gcggattttt ttcgagagag cgcgcgagcg cggcatcgag cgtcaagccg
     1381 cgcgccgcgg gcagactaac gaatgccagc gcgagccaga tgaaaaaaag ccgcttcatt
     1441 ttccctggcc aagcgaagat ggcggacgag cgaattgaaa aacctgctca cccgcagccg
     1501 cgccttttgg ttggtccgcc ttggtcgagc gcacttcaaa agcaagcgtc gcggaatcgc
     1561 ggctcgaggc gcgatcgagt cgcgccagcg ccgcattatg cagatagatc gcactcagcc
     1621 gggtcgtgcg ggtccgcgtc acatcggcag ccgcctggag aacatcgagc tgcgtcccca
     1681 gccccgccgc gaaattgctt ttcgcgaaag tgagcgcatc atccgccgtc tggacgttct
     1741 tcgactgcgc ctggagaacg ctgtcggcct gctgcaaatc gagaaatgcg ctgcgcacct
     1801 cggactcgat cccgagcttg acggccttga gagcaaggag ggcgccgtcc cgccgcgcgc
     1861 gcgtcgcctc catccggccg cgcgtggcgt agccatcgaa gagggcccac gagccattga
     1921 gaccaacgac gtaaccatga ttgaattccg ggccgaccag cgggtcgcgt tcgctgtaga
     1981 cttcgtagcc agtgaaaaat tcgacgtgcg gacgcgtggc actgcgatcg atttccagct
     2041 gcttctcttc gatcgcgaca tctttttgcg cggacctgat ctcgggtcgc tcggccagcg
     2101 cgcgcgccag ggtggcatcg agatcggggt gacgcggttc gtactgcaaa gtgccgaccg
     2161 cttcgaattg cggttcgccg gaggagcggg cgtcgatccc gcaaaggtcg ctcaggtgca
     2221 gataagaatt gcgcaaatcg gtttgcgcct gcaccagctc gggttcttcg ttggcgagcg
     2281 cgacctcggc gcgactggca tccagcgggc caaccgtccc ggcgctcaag cgttcgcgct
     2341 ggcttttcaa ttcctcgcgc agcacgccga ccgattgttc gcggacaagg attttctcac
     2401 gattcaaaag caattcgtag aacgcgaggc gcacatccat cgtcacgcga tcggttacgg
     2461 tttcgaactc gtcctgttgc ttggccaggt tcaattgggc gatcgccacc tgattactgg
     2521 tggcgccgcc ggtgtaaaga ttttggacca cccggaccga ggtgctgtaa tcatccgggc
     2581 gcagccgcgt cgattccgcg tcctcgcgcc ggcgataaag cccactggaa acgaccgagg
     2641 gaagataacc cgagcgcgct tcgacctggc ctccgtgcgc cgcctgaatt ttcttccggg
     2701 cgatcgcgat ttccggattc tggtcctgtg ccagcctaac gacgtcgtcg atcgtgtagc
     2761 ggggcgcgcc ccggacgggg gcgatcgagg gcagcgcaac gagcgccaga agcgcgaggc
     2821 gaaagaccga tcgcatgggt ttttctcaac ggcggcccac tctttcgcaa ctaaattccc
     2881 tcaccacagg atgtcgaacg gctttcgagc cacggcggac cgggaaaatt acggccgcca
     2941 cccaacttct tcgcggctat tcctttaata aatcctgcgc cggggttagg ctggggcgaa
     3001 aaggacgccg cccagtcgca gaagagccgg ttcactttgc atcttgctcc cgatgaatga
     3061 ctgcgccaag gttggcgctt ctcatgagta aaatccctgt cgccattatc ggtgtgggca
     3121 actgcgccag ctcgctggtc cagggaatca gcttttatgg cggtacgccg cgcaacggcg
     3181 cgagcgtcgg cctgatgcat cagcaggtcg ggccctattg gccggacgat attgaagtag
     3241 tggccgcgtt cgatgtcgat cgccgcaaag tggggatcga tgtcagtgaa gcgattttcg
     3301 ctccgccgaa ttgcaccaag attttttgcg aaaaaatcgc gaaaacgggg acgatcgtaa
     3361 aaatgggctg tgtcctggat ggagtgggcg cgcacatgag cggatatgat ccgctgcgga
     3421 cgttccagcc gctcgagaaa gaatcgaccc gcgagcgcat catcgcggag ttggaggaat
     3481 ccggcgccga gatcatggtc aattatctgc cggtcggctc ggaggaagcg acgcgcttct
     3541 acgtgggatg cgcgctcgaa gccgggctcg ccctcgtcaa taatatcccg gttttcatcg
     3601 cgagcgatcc cgtctgggcg aaacgtttcg aggagaaaaa tcttccgctc gtcggcgacg
     3661 acatcaaggc gcagatgggc gcgaccgtcc tccatcgcac gatcgtcgat cttttccgca
     3721 agcgcggagt gaaaatcgag cgcacctatc agttgaacac cggcggcaat actgattttc
     3781 ttaacatgct gaatcgcggc cggctcgagt cgaagaaaac ttcgaaaacg gaagccgtcc
     3841 aatcggtcat cggcgcgcgc ctcgccgacg agaacattca tatcggaccg agcgattacg
     3901 ttccctggca gaacgacaac aaaatctgtt tcattcgcgt cgagggcaag ctctttgggg
     3961 acgtgccgat ggagatcgac ctcaagcttt cggtcgagga ttcgccgaat tccgcggggg
     4021 ttgcgattga tgccattcgt tgctgcaaac tcgcgctcga tcgcgggaaa ggcggcgtcc
     4081 tgcattccgc ctcggcctat ttttcgaagc acccggcgat ccagttgacc gacgacgagg
     4141 cttatcagca cgtcgaggaa ttcatccgcg gcgagcgcga ttcgtagtga tctgtgcggt
     4201 tggagcgatt gtcgccgggg gcgttgaccc cggtcggctc ccggtgccgg gttgtgcgcg
     4261 ccgggatcaa cgctcgcggc tccaatgagc gaagagatct ggctgagctg ccgcgcgatc
     4321 gagcgctacc tgggcgagga tgactcgcgg gtccacgcgc tgcgcggggt ctcgctcgat
     4381 ctcgagccgg gcagcgtcca cgcggtcgtt ggtccttcgg gttgcggcaa gagcaccctc
     4441 ctctatatcc tcggcttgct cgattatccc gacgccggca cgctcacgat cgacggccac
     4501 ctcatggcgc aattgcccga tgacgaggtg gcacggaaac ggaatgagct cctcggtttc
     4561 atcttccaat tccatttcct gctcgaagat ttcaccgcgc aggaaaacgt catgatcccg
     4621 atgcggcggc tcggtcgcct cacggaagag gcgatgctgg aacgcgccgc ccggttgctc
     4681 gaggaggttg atctcggggg gaaacttcgc cggccgagcc ggcatctttc cggcggcgaa
     4741 cagcagcggg tggcgatcgc ccgcgctctg gcgaacgatc cgcacgtcat cctggcggat
     4801 gagccgaccg gcaatctcga caccaagaat tcccgccgag cctttcgctt gttgcagaaa
     4861 atcgtgcgcg aggagcgcaa agctttgctc ctcgtcacgc acaacccgga gatcgcggaa
     4921 gcgtgcgatt ggatccacga aatgcaggac ggcgtgatcg tcggcagcca tccgcgcgga
     4981 caaaggacgg ctttctcgga tgcttaggct ccgggcctgg cggtgtcgtt agggaaaggg
     5041 ctcacggcgg gaaactggac tcgccgcagc gagtcctccg cgatcagaag tcaatgttac
     5101 gcggcgctag cttttccgtt tgaaaagttg atcgagcacc gcgcggtcgt taggctcaag
     5161 tccaattatc cgccaggcga tgaagtatcc ggcgagataa agcgcggccg cggcgaggtc
     5221 cgaccaccac gccggactca cccaacgcag gagcagggcg caggggagag cgaggagcgc
     5281 cgcgatccat ggcttgaaaa gcgcgtgcca tggccagcgc cattcgtagt aatagcggat
     5341 ctcggcccag cgcagcagac ccttgatcat ataggggcac aggacgccga aagccgctcc
     5401 gagcgcgccg taggcgggaa tgagaacgag gttcaatccg acggcggcga cgaaggcgat
     5461 cgtcgagttc atcaggttga tcttcgggcg ttccaccatc aggatcgtct cgcccagact
     5521 aacgaacgcg ttcaacgcgc aaccgcaccc gatgagcgcc gcccagaggc cgccccggta
     5581 aaacgttggt ccgaaaatcg ccatgatcgc accgccggcc agcgccagca cgacgacggc
     5641 tgggagcagg atcgccagca tccagcgggc aagatatccg tagctcgctt cggcttcgcg
     5701 gatttggcgg ccgcccacct gtcgcgcgac gatcggggtg aagatcgggg taaaggactg
     5761 gctcactttg cgcaggccgc ctcctacttc gactgccgcc gcgtaaatcc cgagagtctg
     5821 gagcgtcacc cccggcgcgc ggccgacgaa ccagccgagc atgatgaggt cgatgcgcat
     5881 gatgccgatg ttgagcagat cgtagagcgc gatcggggcc gaggtacgca gcagcgaggg
     5941 caccaagtcg tcgttaggca aaaccgcgcc cgctccctgc gcagtgaaca aacgccgcgc
     6001 gagaaagaaa gcgacgagtc ccgaagccag cgttccggcg atgccggcga attccggcgc
     6061 gagctgccgc gcgccgagcg caaacgcgac cagaagcgca gccgcggtgc cgaagctttc
     6121 cgttaggccg cgcgaataaa tgtcgtgctg catgaccgtc atcccgcgcg agaaagccgt
     6181 gttcacccgg tagagcgtga cgcccggcaa agcgagaagc atgaccgcgc tcgcccgcgc
     6241 aaactccggt cgcaggccgg ccgcgggccc gaaagtccag agcagaaaaa aaccgccgac
     6301 ggccagcaac acgctgctgg tgcaggagac gatcagcgcc gcccgtcgca cccggcgact
     6361 gctctcgcgg tcccccgtcc ctttcgcttg ggcgatgaac gcaatcgcgc tgtaatcgaa
     6421 gccgagcgtg cagaatttgc tcaccaaatc catcgtcgcc caggccagac cgaagatgcc
     6481 gaggactgcg ctgccgagga gccgcgcgac aaggaaggtg aaaattccgc gcagattcga
     6541 agcgagaaac gccagcgtgt tgatcgcggc gccgcgcgcg agcgttgccc cgtgagagga
     6601 agagtctttg tccatctcag tcaccggttc gttgcgactg cgtcgtggta cactgccagc
     6661 gtttctttgg cacaccgatc ccacgaaaaa tcccgggaac gcgccaggcc ctggccggcg
     6721 agagactccc tcaatccggg cgactgaacg aaatcgcgca aggcgttcgc cagcccggtg
     6781 acgtctcccg ctttgaagag aagcgcagtt cccgccgtca cctcgcggag cggcggaata
     6841 tcgctcgcca ccaccgggca accgcaggcc atcgcctcca agaccggcaa accgaagcct
     6901 tcgtaaacgg acggctgaat caatccgcca gcggcctgca agagcgtgac caaatccggc
     6961 cgtgccaccg tttccagcca gaccacgcga tcgtcgacct ggagttgttt cgccaatcgc
     7021 acgagccctt tgctggcctt gcgccgttgc accacaacga ggagccaggg gcggggaacc
     7081 gccctgccga aagcggcgag cgccagatca tgccgcttgc tcggagtgtt cgcgcccacc
     7141 agcagcagat agggtgcttc acttttcgtt aggcgggcgg cgcgttcctg cgccgccgct
     7201 gggtcgttag gcgggccgag atcgcggtcc ggcgccgacc agatgacggt cagccgtttg
     7261 gccgcctccg ggagcaacgc gcaaatgcga tccgccgtcg cccggctttg cgcgaccaac
     7321 cgagtcgctt cccggagcgc gcgccagacg gcctgccggt agtaaacgct cttcaccacc
     7381 cgctcgagcc cctgcaagtg catcgccggc tcttcgagcg ccatcacatc gtgcaccgtg
     7441 atcacactcg cgcagggcag accacgcggc atcaggttgt gcggactgtg gaaaacatcg
     7501 acctctgcga acgaagcgta atgctcgggc caaaggaccg gccacggcga attcggtccg
     7561 gccttcacga tcgtggccga agtattcggc gcgggcgaaa gcggccgtgg tgccagcgga
     7621 tgggcccaga aaaaaaaatg atcggacgcc gccagggagg gcagccgttc gaccagcgcc
     7681 tgcacatagg cgccgatccc gctgggtttt tcgcgcacgt agcgcgcgtc gatcgcgatg
     7741 cggagcgcgc gagatggcga agaagattcc atggtgggta cgagaactcg ttcgactcgg
     7801 ttgcgaacga agcctttcaa tcagataaag tccgagctta caactggaag cgaaccgatc
     7861 gggatgaaga aaatttttct cactggcgcc agctccggca tcggattggc catcgcccaa
     7921 aaactttgtg ccgccggtca cgaagtttgg ggaacttcgc gcgacccggc ccgcatcccg
     7981 tcgttaggca agctccaccc ggtgcacctc gatctggccg agctggaggg aattcgcggc
     8041 gccttcgaaa attcctggcg cgaagccggc tacttcgatg tcctgatcaa caacgcgggc
     8101 gggggacatt tcgacgccgc ggaaattctc ggaccggaaa ggatcgaaga gttgttccgg
     8161 accctggtct ttgcgcacat cgagctttgc cggctggccc tcggcgcgat gaacgcgcgg
     8221 caatccgggc tgattatcaa cgtgacatcg ctcgcctcac gtcttccggt gccctacatg
     8281 tccgcctaca acgcggccaa ggcggcgatg gcttcctaca cgttttcgct ccagctcgag
     8341 ccccggaatc gccgggtgcg aattgtcgat ctgcagccgg ccgacatccg cacgagcttc
     8401 aacgactcca tttcgccgca gccaccaggc gacaggcacg tcgccaaaac gtgggaggtt
     8461 gcccagagaa acatgaggaa tgcgccgccg cccgatctcg tggcgtgccg ggtcctgcgt
     8521 ttgcttgcat ccgccaaccc gccgccacgc ctgacggtcg gcggtgtttt tcaatcacgg
     8581 atcgcgccgc tgatctttcg cttcctgccg caacgagtgc gggtttgggg attgaaaaat
     8641 tattacggaa tctagcgtgc gcttcgcagc ccgtgcgacg atcaaccaag gcaaatagga
     8701 gcaagagcct cggaaaagaa aaacctccgc gcgcccgcct caggcgggaa gcgagtccaa
     8761 agctccccgc aaatcttcaa cccaacaaac caaagaacta aatcctacca aacccgccac
     8821 cgcgcttgac tcccttaggg ttagctgttg tcggccgcca ccgtttcgac ccactgcgcg
     8881 cgccaaccag gggcgttgaa tgggtcggca aagtattggg tttcatgcga cacttttcca
     8941 tcccgaaact ccatgatgct cacggtgtag acgcgcttgc cttcgtagat aatcacatac
     9001 tcagtgatcc agagattgct ctttcccagg atgcggcgaa tcgaaaaccc agatggcctg
     9061 ccgggatgat gactacgcag cgcctgcagg ttactccgac cgcgaattat ctcgcctgat
     9121 tgtggatact cgcacacgat gtcatcgtgg tagatatcat gttcggcaac ttgatcgccc
     9181 gcagcagacg ctgcccaatg ccgatcaatg gccatccgaa tgttctgctc ttccattagc
     9241 ggcctccttg agcggttacg cgttcatgaa actgaacttc gtcgacatct agaccttatg
     9301 gctgcgggcg cggggtgggt gcgcgagcgg cgggttgaaa aggcgggtgt gtttgccgtt
     9361 catattttct cgcgggctac tccggttgag ggagcagagc cgcaccaacc ccgaagggtt
     9421 cggggttgca gtttaacaag tcgaacaagc aaacacacca atggaaaagg aactctatct
     9481 gggactggat gtccataaag actgtatcgc cgcggcggtg gccgagagcg ggcgcactgg
     9541 agaggtgcgc gacagcggtc atcagcaatg atctccaggc gctggagaag cgggtcgggc
     9601 ggccgcgcaa agcgcaaggc aaaacagtcc gcctgctcga agtggaaata ttgtagtcat
     9661 ctgaaatgta aacgcgtgac aggtgccggt tagctccact gattggttag gcgttgcacg
     9721 agactgctag tgccctgtat cacttgccca tctagcagcc attcctcctt ttcgcgcctt
     9781 gtccgtcgct gtgcatatac gtgactcacg tatatcaaac
//
LOCUS       DATMNS010000037         6704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_47380, whole genome shotgun sequence.
ACCESSION   DATMNS010000037 DATMNS010000000
VERSION     DATMNS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47380"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1319
                     /locus_tag="VNW28_01440"
     CDS             <1..1319
                     /locus_tag="VNW28_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HXA08613.1"
                     /translation="PWYRPSLTIQIFIGLIAGGFIGWLRPEWGNKVYFLRDIFLNLIK
                     AIIAPLVFSTIVAGIAGAGSLKKVGRMGLKAIIYFEIATTAALFIGLAVVNFTKPGVG
                     VALIAGPNDIVNTVAHTHPRTLLETIVHIFPASVIDAMARGDVLQIVAFSVLFAMAVS
                     AVGEKGKPIVRAMESLMQVMFKFTNYVMMFAPIGVGAAMAHTIANQGLGVLFNLGNLI
                     LSLYAALVILTVLVFGGVILLLRIPLRQFVRAVREPATLAFATTSSESALPKAMESME
                     RFGVPADIVGLVMPTGYSFNLTGSTLYLAMASVFVAQAAETTTGHHIGLGQQLTTMLA
                     LMITSKGVAGVPRSSLVILLATLNSFLPAGLGPLGVAVIFGVDELMDMGRTCVNVIGN
                     CLGTVVVARWEGEFNDERARVFGTPQEIELDLREGEIAFAEAARHD"
     gene            1312..2679
                     /locus_tag="VNW28_01445"
     CDS             1312..2679
                     /locus_tag="VNW28_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXA08614.1"
                     /translation="MTSWRARWKAFRFPIDPEKQALLRARWESLPNELRTPNQLAGRH
                     LTHCGFTTGASYCSFRCTHCYLPKNANEVPIPSLADMKEQIEANRRLQGPGGGLQITG
                     GDVADAYWRGGRQAELVEIVRHAVAVGLVPMLMTHGQTLLEHPEFLEALVLEGGLRQV
                     AVHIDLTQAGRHGFPINRLTSESDLHPVREAFTNLARKIRTRTGFPFELAHNCTVTER
                     NIDSVPEVVRWFLADPARSRIWRILSFQPEANTGRTIFSQHPVTPALVWEKICAGMGL
                     QLDGSAYLGGHPDCNQGASLLVEQRRGRYFPLLPNDERTRRLFADILAAIGAISTMTT
                     DSDGGASLLPYRVAGAFARHPRLAGRSLARAAALILSGQIPVTFLRALVTGRAHTINI
                     GTHNFMDASRVANASNDPVTRARLEACVFKGAVKNRATGEWETVPMCAMNESRWSELY
                     AERLA"
     gene            complement(2780..4222)
                     /locus_tag="VNW28_01450"
     CDS             complement(2780..4222)
                     /locus_tag="VNW28_01450"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXA08615.1"
                     /translation="MKTLILGVLLCLTTLPSAFAIGGVDAPPAPSATHETKFATAKET
                     RLDNGLRVIVTERPGLPLLAAQVLVRSGAEVDADGYAGTASLTGDLLTKGTDTMSAPE
                     IASAIESLGGSIDSGAGWDASAATVVVISNKADEALKILADTVRHPAFKQEEIDRLKN
                     QALDGLRVALQQPGSLARFVTTRVVFGSGAYGHAAGGTLETVQAIGRDQITQLYQKYY
                     QPRNAAFILAGDITLAQGKAFAEKYFGDWKNREAAPNDKVRSGAADWKPSGVVIDMPE
                     AGQAAVTMARPAIARASPDYYSAQVANAALGNGFVSRLNREIRIKRGLSYGAGSSIDP
                     RREEGPFAASAQTKNQSAAEVAGLMESELKRLVSDPVKGEELKSRQAVLTGRYARNLE
                     TNRGFVSEISDLATYDLPLDTLDKYIPSIDAVSSEAVTKFAEKYLAQPTSLVIVGKAG
                     DFLEALKKSFPDVKVIEQKDLDLNRADLTK"
     gene            complement(4324..5706)
                     /locus_tag="VNW28_01455"
     CDS             complement(4324..5706)
                     /locus_tag="VNW28_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXA08616.1"
                     /translation="MKFSPALAALLIASLPIARAADPEPAKSAVAVPPLNFQHETLPN
                     GLEIYSVEDHSSPTVAVQVWYHVGSKDDPAGRSGFAHLFEHMMFKGNEHLTPDTFDNL
                     TENIGGENNAYTAEDVTVYHEVVPSNYLQPILWAEAERMSSLALNETNFNSERDVVKE
                     EYRQRILANPYGEFFLDTVRNSYAVHPYKRPGIGNIAELDASKLPEVRAFHATFYRPD
                     NATLIVVGDFQPNELHDWVDKYFGAIKKPAEKIPRVTAQEPPRKEDKTIVKYSPKVPL
                     PAIAVTYLAPSLRSQDAPALELADEILSGGESSRLYQALVYDQQIAQQVSFNADLNQD
                     LGLLTARMILASGKTPADAEKALNAQLDKILKDGVTPAELEKAKNRYLTGKLVERETN
                     NGKASALGEAVVLYGDPNRVNTDLGKLQAATADQVKEAMNRYLSGKKKVVIEYLPEAM
                     KANGQMTNAK"
     gene            complement(5777..5850)
                     /locus_tag="VNW28_01460"
     tRNA            complement(5777..5850)
                     /locus_tag="VNW28_01460"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5814..5816),aa:Ile,seq:gat)
     gene            complement(5903..6361)
                     /locus_tag="VNW28_01465"
     CDS             complement(5903..6361)
                     /locus_tag="VNW28_01465"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXA08617.1"
                     /translation="MRFSAVFKSWLPVLLWMALMFTASTDLLSAEHTSRFLVPFLRWL
                     KPDISWETMMQIHFLVRKGAHLIEYAILAVLLWRAFRFRHTSGRSLGPSAALALAVAI
                     CFAASDEYHQSFVPSRGASSSDVMIDSCGAVLGLALRWGIGGRKPKSAAP"
     gene            6429..>6704
                     /locus_tag="VNW28_01470"
     CDS             6429..>6704
                     /locus_tag="VNW28_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08618.1"
                     /translation="MKTELPPPLVFEPLFMERMWGGRRLESVFGKKLPPGKCIGESWE
                     VVDRPETQSVVRQGPWRRRTLHELWSDHRSEIFGPAVPDAPRFPIFAK"
BASE COUNT         1229 a         2053 c         2052 g         1370 t
ORIGIN      
        1 ggccctggta ccgaccgtcg cttactatcc agattttcat cgggctcatc gccggcggat
       61 tcatcggctg gctgcgtcca gagtggggta acaaagttta cttcctccgc gacatttttc
      121 ttaacctcat caaggcgatc atcgcgccgc tcgtcttctc gaccatcgtc gccggcatcg
      181 cgggcgcggg gagtctgaag aaagtgggcc ggatgggatt gaaagcgatc atctacttcg
      241 agatcgcgac cacggcggcc ctcttcatcg ggctcgccgt tgtcaatttc acgaaaccgg
      301 gcgtgggcgt tgcgctaatc gccgggccta acgacatcgt caacaccgtc gcccacaccc
      361 atcctaggac gctcctcgag accatcgtcc acattttccc cgcgagcgtg attgacgcca
      421 tggcgcgcgg cgacgtgctc cagatcgtcg ccttctcggt cctctttgcg atggcggttt
      481 cggcggtggg agaaaaaggc aaaccgatcg tgcgcgcgat ggagagcctg atgcaggtga
      541 tgttcaagtt caccaactac gtcatgatgt ttgcgcccat cggggtcggc gcagccatgg
      601 cgcacacgat tgcgaaccag gggctcggcg tgctcttcaa cctgggtaat ctcatccttt
      661 cgctttacgc cgctctcgtg attctcactg tcctggtctt tggcggcgtg atcctgctcc
      721 tccgcattcc gctgcgccag ttcgttaggg cggtgcgcga accggccacg cttgctttcg
      781 ccaccacgag cagcgagtcg gctctcccca aggcgatgga atcgatggag cgcttcggcg
      841 tgcctgcgga catcgtcggg ttggtcatgc ccacgggcta ctcgtttaac ctcaccggct
      901 cgacgctcta cctggcgatg gcttccgttt tcgtggccca ggcggcggaa acgaccaccg
      961 gccatcacat tgggctcggg caacagctta ccaccatgct cgcgctcatg atcacctcga
     1021 agggcgtggc cggcgtgccg cgttcttcgc tcgtaatttt gctcgcgacc ttgaacagtt
     1081 tcctgcctgc cggactcggt ccgctcggtg tggccgtcat cttcggcgtc gatgagttga
     1141 tggatatggg ccggacctgc gtaaacgtga tcggtaactg tcttggcacc gtcgtcgtgg
     1201 cgcgatggga aggggagttc aacgatgagc gggcgcgagt ctttggaacg ccgcaagaaa
     1261 ttgagctcga cttgcgcgaa ggcgagatcg catttgcgga ggcggcgcgc catgactagc
     1321 tggcgcgcgc gctggaaagc gttccgcttc ccgatcgacc ccgagaaaca ggcgttgctg
     1381 cgggcccgct gggaatcgtt gcctaacgaa ctgcggacgc ccaaccagct cgccggccgt
     1441 catctcaccc actgcggatt caccactggc gcgagctatt gctcctttcg ttgcacccat
     1501 tgctatctgc cgaagaacgc gaacgaggtc ccgatcccgt cgctcgccga catgaaggag
     1561 cagatcgaag cgaaccgccg cttgcagggg ccgggcggcg gattgcagat caccggcggc
     1621 gatgtcgccg atgcctactg gcgcggcggc cgccaggcag aattggtcga aatcgtcagg
     1681 cacgccgttg ctgtcgggct cgttcccatg ttgatgacgc acggccagac tttgctcgag
     1741 catccggaat ttctggaggc actcgtgctc gagggcgggc tccgccaggt cgccgtccat
     1801 atcgatctga cccaggccgg tcggcacggt tttccgatca atcgcctgac gagcgaaagc
     1861 gatctgcatc cggtgcggga agcgttcacg aacctggcgc ggaagattcg cacgcgcacc
     1921 ggatttccct tcgagctcgc gcacaattgc accgtcacgg agcggaacat cgattcggtg
     1981 cccgaggtcg tgcgctggtt tctggccgat ccggcgcgct cgcgcatctg gcgcatcctg
     2041 agttttcagc cggaggcgaa caccggacgc acgatttttt cccagcatcc cgtgacgccg
     2101 gcgctcgtct gggaaaaaat ctgtgccggg atgggattgc aactggacgg gagcgcctac
     2161 ctcggcggtc atccggattg caaccaggga gcgagcctgc tcgtcgagca acggcgcggg
     2221 cgttatttcc cgcttttgcc taacgacgag agaacgagac ggctcttcgc cgatatcctg
     2281 gccgcgatcg gcgcgatctc gaccatgacg accgactccg acggcggggc gtcgttgctg
     2341 ccctatcggg tcgcgggcgc cttcgcgcgt catccgcgac tggccggccg cagcctggcg
     2401 cgtgcggccg ccttgatttt atccggccaa atcccggtga catttctccg ggcgctcgtg
     2461 accggacgcg cccacacgat caacatcggg acgcataatt tcatggacgc gagccgggtc
     2521 gcgaatgcgt cgaatgaccc cgtgacccgt gcccgcctcg aggcttgcgt cttcaagggc
     2581 gcggtcaaaa atcgcgcgac cggagagtgg gaaacggtcc cgatgtgcgc aatgaatgaa
     2641 tcgcgctgga gcgagttata cgctgagcga cttgcgtaac acagccgtct cggctgtcac
     2701 gtcatgcggg cgtctcgcct gcataatcga caacctgcgg gcgagacgcc cgcactacga
     2761 gacaggctgg aagcctgtgt tacttcgtca ggtcggcgcg attcagatcg aggtcttttt
     2821 gctcgatcac tttgacgtcg ggaaaacttt tcttgagcgc ctcgagaaaa tctccggctt
     2881 tgccaacgat gacgagactg gtcggctggg cgagatattt ctcggcgaat ttggtcaccg
     2941 cctcgctcga gacggcatcg atcgacggga tgtatttatc gagcgtatcg agtggcagat
     3001 cgtaggtcgc gagatcggag atctcggaaa cgaaacctcg attggtttca agattccgcg
     3061 cataacgacc cgtcagcacg gcctggcggg acttcaattc ctcgcctttc accgggtcgc
     3121 tcacgaggcg tttcagttcg gactccatca gaccggccac ctccgcggct gactgatttt
     3181 tcgtctgagc cgaagcggca aacggaccct cttcgcgtcg tggatcaatg gagctgccgg
     3241 cgccgtaact cagcccgcgc ttgattcgaa tttcacggtt gaggcgcgag acgaagccgt
     3301 tgccgagcgc ggcgttggcc acctgcgcgg aataataatc cggcgaagcg cgggcgatgg
     3361 cgggccgcgc catcgttacg gcggcctggc ccgcctccgg catatcgatc acgaccccgc
     3421 tcggtttcca atccgccgcg ccggagcgaa ccttgtcgtt aggcgcagct tcccggttct
     3481 tccagtcgcc aaaatatttt tcggcgaacg ccttgccctg cgcgagcgtg atatcgcccg
     3541 cgaggatgaa ggccgcgttg cggggctgat aatatttttg gtaaagctgc gtgatctggt
     3601 cacggccgat cgcctgcacg gtttccagcg tgccgccggc cgcatgacca taggcgcccg
     3661 aaccaaacac cacccgggtc gtgacaaaac gcgcgagcga gcccggctgt tgcaacgcga
     3721 cccgcaggcc atcgagcgcc tggtttttca agcgatcgat ttcctcctgt ttgaaggccg
     3781 gatgccgcac cgtgtcggcc aggatcttta aggcctcgtc cgccttgttc gatatcacga
     3841 ccacagtcgc agccgacgcg tcccagcccg cgccggaatc gatcgacccg cctaacgact
     3901 cgatcgcgct cgcgatctcc ggcgccgaca tggtgtcggt tcccttggtc agtaagtcac
     3961 cggtcaggct cgcggtgccc gcgtatccgt cggcgtcgac ctcggcgccg cttcggacca
     4021 acacttgggc ggcgaggagc ggaagtcccg gccgctccgt cacgatcacg cgcaatccat
     4081 tatccagtct cgtctccttc gccgtggcga actttgtctc atgcgtcgcg gaaggcgccg
     4141 gcggcgcgtc gaccccgccg atggcgaagg cagatggaag agtcgtcagg caaagaagca
     4201 caccgagaat aagcgttttc ataccaaaac ccgaatgtcg aaatccgaag gaattccgaa
     4261 gcccgaagtc cgaattgcgc ttccgcctga tcgttttttg attcgtcatt ggttcgccct
     4321 tcgtcatttg gcattcgtca tttggccgtt cgccttcatc gcttccggca aatattcgat
     4381 tacgactttc tttttcccgg agagatagcg gttcatcgct tccttcacct ggtcggccgt
     4441 cgcggcctgg agtttaccca ggtcggtgtt cacgcgattc ggatctccat agagaacgac
     4501 cgcctcaccg agcgcgctcg ccttgccgtt gttggtctcc cgctccacca actttccggt
     4561 gagataacga ttctttgcct tctccagttc cgcgggcgtg acgccgtctt tcagaatttt
     4621 gtcgagctgc gcatttaacg ccttttccgc atccgccggc gtctttccgc tcgccaggat
     4681 catgcgcgcg gtgagcaggc cgaggtcctg gttcaagtcg gcgttgaagg aaacctgctg
     4741 cgcgatctgc tggtcgtaga cgagcgcctg atacaagcga gatgattcgc caccggacaa
     4801 aatttcgtcc gccaattcca gagccggggc gtcctggctc cggagggagg gcgcgagata
     4861 agtcaccgcg atcgccggga gcggcacctt cgggctgtat ttgacaatcg tcttgtcttc
     4921 cttgcgcggc ggctcctgcg ccgtcacgcg cgggattttt tccgcgggtt tcttgatcgc
     4981 gccgaaatat ttgtccaccc agtcgtgcaa ttcgttaggc tgaaaatcac cgactacgat
     5041 gagggtggcg ttgtccggcc ggtaaaaggt ggcatgaaag gcgcgcactt ccggcagctt
     5101 ggacgcgtcg agctcggcga tgttgccgat ccccggccgc ttgtagggat ggacggcgta
     5161 ggaattcctt accgtatcga gaaaaaactc gccgtaggga ttggccagaa tccgctggcg
     5221 atattcctcc ttcaccacat cgcgctccga attgaaattc gtttcgttca gcgcgagcga
     5281 cgacatccgc tcggcttccg cccacaggat cggctggaga taattcgacg gcacaacctc
     5341 gtgataaacc gtcacgtcct cggcggtgta ggcgttgttc tcgccgccga tgttttcggt
     5401 caggttgtcg aacgtgtccg gggtaagatg ttcgttcccc ttgaacatca tgtgctcaaa
     5461 caaatgcgcg aacccgctgc ggcccgccgg gtcatccttt gagccgacat gataccagac
     5521 ttggacggcg accgtcgggc tggaatggtc ttccaccgag tagatctcga gaccgttcgg
     5581 caaggtttcg tgctggaagt tcaggggcgg caccgcgacc gccgacttcg cgggttccgg
     5641 atcagccgcg cgtgcgatgg gaagggatgc gatgaggagc gcggcgagag caggagaaaa
     5701 tttcacgatg ggttcgacac ctgacagaca acttcgttgc gagaatcccc agatcgtgtc
     5761 ggctcgcggg cgccaatggg cctgactggg ctcgaaccag tgaccctgcg cttatcaagc
     5821 gcatgctcta accaactgag ctacaggccc gcgaggcgcg gaacgccaag ggagtgaata
     5881 gtgaagggtg attggtgatt ggtcaaggcg cagccgattt cggttttcgc ccgccgatcc
     5941 cccagcgcaa ggcgagcccc aggaccgcgc cgcaactgtc gatcatcaca tcgctggacg
     6001 acgccccgcg gctcggcacg aaggattgat gatattcatc cgatgccgcg aagcagatcg
     6061 ccacggcgag agcgagcgcc gcggagggcc caaggctgcg tccgctagtg tgacgaaaac
     6121 gaaacgcccg ccaaagcaag accgcgagaa ttgcatactc gatcaggtgc gcgcccttcc
     6181 ggacgaggaa atgaatctgc atcatcgtct cccaggagat atcgggcttc agccaacgga
     6241 gaaacggcac caggaaacgc gatgtgtgtt ccgccgagag aaggtcagtc gacgcggtga
     6301 acatgagcgc catccagagg agcaccggaa gccaggattt gaagacggcg gagaaacgca
     6361 agccgggagt gtaatcggcg gagggtgtct gacaaaccca gtgtcccggg ttaatctcgc
     6421 cgccggaaat gaaaactgag ttgccgccgc cactggtctt cgagccgctt ttcatggagc
     6481 ggatgtgggg cgggcggcgt ttggagagtg tttttggcaa gaagcttccg cccgggaagt
     6541 gcatcggcga atcttgggaa gtcgtcgatc ggccggaaac gcagagtgtc gttaggcaag
     6601 gcccgtggcg ccggcggacg ctgcacgaac tttggtccga tcatcgtagc gaaatctttg
     6661 gtcccgccgt gccggacgcg ccgcgtttcc cgatctttgc caag
//
LOCUS       DATMNS010000038         2725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3961592, whole genome shotgun sequence.
ACCESSION   DATMNS010000038 DATMNS010000000
VERSION     DATMNS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3961592"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="VNW28_01475"
     CDS             complement(<1..840)
                     /locus_tag="VNW28_01475"
                     /inference="COORDINATES: protein motif:HMM:TIGR02429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase"
                     /protein_id="HXA08619.1"
                     /translation="MSKVYSDAKAALAGLLHDNMTIAAGGFGLCGIPENLIAALHDSG
                     VKGLTIVGNNAGVDGFGMGVLLTTRQVKKVMASYVGENKEFERQVLAGELDLELIPQG
                     TLAERLRAGGAGIPGFYTRTGFGTKLTEGKETKVFEGKEYVLEPGIKVEVSLVKAWKG
                     DKSGNLIFRKTSRNFNPMIASCGRVCVAEVEHLVEAGELDPDQIHTPGIFVDRIIQGA
                     KYEKRIEFRTVQGGAAAKKENPIRELMAKRAARELRDGYYVNLGIGIPTLVANYIPPG
                     MNVT"
     gene            1099..2496
                     /locus_tag="VNW28_01480"
     CDS             1099..2496
                     /locus_tag="VNW28_01480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXA08620.1"
                     /translation="MSKSLAHRLAEYSCSLIFEDLSPEVVHEVKRRVIDSFGCALGAW
                     HEDPCVIARKVASDFSARNGATVIGTDHQAPPDWAAFATGCCIRYFDYNDTYLSKEPA
                     HPSDNIAAALAVAESVGANGRELITAIALAYEVQCRFCDAASIRARGWDHPTYGAFST
                     ALAAARLMKLDPEKTRHAVNIAGVNCAAFRQARVGELSHWKGVAFANAARHGVYAALL
                     ARAGMTGPAPIFEGQMGFEKELGVSLGNVGEVFVKRPNEKNVNEGTAAMILKTSIKYW
                     PAEYHSQSAIEAALFLRQEIGDPAKIQSVRIESHDASVDIIGSEPEKWKPETRETADH
                     SLPYITAIALLDGEVTEKQFQPARFMDSKIWKFLESVTVERNAELTSLYATAVANIVH
                     VTLQDGRVLTKRVDYPLGNAFNPVSDRQLEGKFNALVVPELGEEGATKILAAGWKLEE
                     TSNFHDLMKLARMPA"
     gene            2511..>2725
                     /locus_tag="VNW28_01485"
     CDS             2511..>2725
                     /locus_tag="VNW28_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08621.1"
                     /translation="MPRDSSTSLRLTSRLRQLIARGVVALPGVPNASMARQVERAGFD
                     AVYISGAGLANCTAGLPDVGLLTLNEVA"
BASE COUNT          514 a          790 c          850 g          571 t
ORIGIN      
        1 ggtgacgttc atgcctgggg gaatgtaatt ggccaccagc gtcgggatgc cgatgccaag
       61 gttgacgtaa taaccgtcgc gcaattcccg cgccgcgcgt tttgccatca attcgcggat
      121 ggggttttct ttcttggccg ccgcgccgcc ctgcacggtg cggaactcga tccgtttttc
      181 gtatttcgcc ccttggataa tgcggtcgac gaaaatgccc ggcgtatgga tctggtcggg
      241 atcaagttca cctgcctcga cgagatgctc tacttcagcg acgcagaccc tgccgcagga
      301 cgcgatcatg gggttgaagt tccgcgaggt ttttcgaaaa atgaggttgc cggatttgtc
      361 gcctttccac gctttcacga gcgaaacctc gactttgatg ccgggctcga gcacatactc
      421 cttgccctcg aaaacttttg tttccttgcc ttcggtcaat ttggtgccga agccggtgcg
      481 ggtgtagaac cccgggatgc ccgcgccgcc ggcgcgcaac cgctcagcca gtgtgccctg
      541 cgggatgagc tcgagatcga gttcgcccgc cagcacctgg cgttcgaact ccttgttctc
      601 accgacgtag gaggccatga ctttctttac ctgccgggtc gtgaggagaa cgcccatgcc
      661 aaagccatcc acgcccgcgt tgttcccgac gattgtgagg cctttcaccc cgctgtcgtg
      721 cagggcggcg atgagatttt ctgggatacc gcagagcccg aacccacccg cggcgatcgt
      781 catgttgtcg tgaaggagac cggccagcgc ggcctttgcg tcggagtaaa ctttgctcat
      841 ttggagagaa gtttaaccat ggattacacc gatgggcacg gatgaattgc cgcgagagcg
      901 atatgcgagt tgtagacgca tcccctgtgg gaggcgcgag tgtcgggatc gatcttctct
      961 ctgccagcga cgtcttctcc cgcgcttcgc acagcgaagc ggctacagcg cgaagcgtga
     1021 ttgcgacgcg tgattgtaac ggtgattgtg acgccagctt gccgcgcgga gattcatcgc
     1081 cagattgcaa gcgctctcat gtccaaatct ctcgcccacc gtctcgctga atattcctgt
     1141 tcgcttattt tcgaggatct ttcgccggaa gttgtccacg aggtgaagcg gcgggtgatc
     1201 gattccttcg gttgcgcgct cggcgcctgg catgaggatc cgtgcgtcat tgcgcgtaaa
     1261 gttgcctccg atttctcggc caggaatggc gcgaccgtca tcggcaccga tcatcaggcg
     1321 ccgccggatt gggcggcctt cgcgaccggc tgctgcattc gttatttcga ttataacgat
     1381 acctatcttt cgaaagagcc ggcgcatccg agcgacaata tcgccgccgc tctcgcggtc
     1441 gccgaaagcg tgggcgcgaa tggtcgcgag ctgatcaccg cgattgcgct ggcctacgaa
     1501 gtgcagtgcc gtttttgcga tgccgcgagc attcgcgcgc gcggctggga tcacccgacc
     1561 tacggcgctt tctccaccgc tctggcagcc gcgcgtttga tgaaactcga cccggaaaag
     1621 acgcgccacg cggtcaatat cgcgggtgtc aattgcgccg cctttcggca ggcccgggta
     1681 ggggaacttt cgcattggaa aggcgtcgcc tttgccaacg ccgcgcggca cggcgtctac
     1741 gccgcgcttc tcgcgcgggc cggaatgacg gggccggcgc cgattttcga ggggcaaatg
     1801 ggattcgaga aggaactcgg ggtgtcgtta ggcaatgtcg gcgaggtctt tgtcaaacgc
     1861 cctaacgaaa agaacgtgaa cgaaggcacg gctgcgatga ttctcaagac cagcatcaag
     1921 tattggccgg ccgagtatca cagccagagc gcgatcgaag ccgcgctctt tctgcgccag
     1981 gagatcggcg acccggccaa aattcagtcg gttcggattg agagtcacga tgcctcggtc
     2041 gacatcattg ggagcgaacc ggagaagtgg aaaccggaga cgcgcgagac ggcggatcac
     2101 agcctgcctt acatcaccgc gatcgcgctg ctcgatggcg aggtaacgga aaagcagttt
     2161 cagccggcgc gtttcatgga ttcgaagatc tggaaattcc tcgaaagcgt aacggtggag
     2221 cgcaacgccg agctcacgtc gctttacgcc acggcagtgg cgaacatcgt gcatgtcacg
     2281 ttgcaggacg gccgggtgct gaccaagcgg gtcgattatc cgctgggcaa tgccttcaac
     2341 ccggtgagcg accggcaact ggaaggaaag ttcaacgcgc tggtcgtccc ggaactgggc
     2401 gaggagggcg ccacgaaaat tttggcggcg ggttggaaac tggaggagac gtccaacttt
     2461 cacgacttaa tgaagctggc gcggatgcca gcctaacgaa ctgtttgact atgccgcggg
     2521 attcctcgac ttcgctccga ttaacgagcc gcttgcgcca gctgattgcg cggggcgtag
     2581 ttgccctgcc aggcgtgcca aacgcctcca tggcgcgtca ggtggaacgc gcgggattcg
     2641 acgccgttta catcagtggc gcggggctgg cgaattgcac tgccggcttg cccgatgtcg
     2701 ggctgctgac tttgaatgaa gtcgc
//
LOCUS       DATMNS010000039         4260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2555771, whole genome shotgun sequence.
ACCESSION   DATMNS010000039 DATMNS010000000
VERSION     DATMNS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2555771"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VNW28_01490"
     CDS             complement(<1..359)
                     /locus_tag="VNW28_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXA08622.1"
                     /translation="MELRAALKKHFGYSAFRPLQEEIVRDALAGRDVFALMPTGGGKS
                     LCFQLPALLREGLTIVISPLISLMKDQVDALQASGVAATFLNSALDGAQARSRLRGLY
                     NGEFRLLYVAPERLMLES"
     gene            complement(407..1549)
                     /locus_tag="VNW28_01495"
     CDS             complement(407..1549)
                     /locus_tag="VNW28_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA08623.1"
                     /translation="MKRHPKFSFGRLRGIIVKEFTQMRRDRLTFAMMLGVPLIQLTLF
                     GYAINSDPRHLPAAVVLADRGPYGRTLLYAMKNSTYFNFVEQVATEEEAHNLLARGEV
                     QFVINIPQNFTRDLLRGDQPAILVEADASDPVATGNALASLGNVINTALQQDLKGSLA
                     SLNATAGPVDVRIHALYNPEIITQYNIVPGLMGVILTMTMIMITGLAITRERERGTME
                     NLLSMPTRPSEVLIGKIVPYIMIGYIQVGVILVAAHFLFHVPMQGSIPLLLAVALVFI
                     AANLAMGITFSTIAKNQLQAMQMTFFVFLPSILLSGFMFPFRGMPPWAQAIGEFFPLT
                     HFLRIVRGILLKGNAFNDVLLQLWQIALFALIALIIGVKRYRQTLD"
     gene            complement(1546..2472)
                     /locus_tag="VNW28_01500"
     CDS             complement(1546..2472)
                     /locus_tag="VNW28_01500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA08624.1"
                     /translation="MNGDSAIDVRGMTKRFGERAVVDHVDLHVHTGQIYGFLGPNGSG
                     KTTFLRMLCGLLRADDGSGRVLGYDVIAESEAIKREVGYMTQRFSFWEDLSIEENLDF
                     VARMYAVKNRRATVKESIAQLGLAGRQKQLAGQLSGGWKQRLALAACLIHKPKLLLLD
                     EPTAGVDPKARRDFWEQIHQLSAQGLTFLITTHYMDEAERCHRLAYISFGKLLADGSV
                     DDVITQVGLTTWSVSGPDLPHLAEQLRALSGVQQAVAFGNALHVSSDDAAALKQAIAP
                     FRHPPYDWREIPSGLEDAFISLMEGSRDNFTP"
     gene            complement(2469..3245)
                     /locus_tag="VNW28_01505"
     CDS             complement(2469..3245)
                     /locus_tag="VNW28_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXA08625.1"
                     /translation="MKTNRILHRVVCLTSGVLLLAGCRKAPSDRVQGYVEGEYVYVSS
                     PLGGILDHLDVQRGQQVTADQPLFSLDPTMELAARDQARAALALSEATFKRQQQLFHE
                     GPSAAQDLDNARAVRDQNQQQLAQAEWNLSQMKQSAPQAALVYDTLYRQGEFVPAGKP
                     IAALLPPPNIKVRAFVPETRIGSIQPGDTARVTVDGVSAPFIGRVSYISPRAEYTPPV
                     IYSQESREKFVFMVESVFDPVTAAKLHPGQPVDVQFEPKK"
     gene            complement(3242..3895)
                     /locus_tag="VNW28_01510"
     CDS             complement(3242..3895)
                     /locus_tag="VNW28_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXA08626.1"
                     /translation="MKTNKRTARSTSIAAARSAPPSALSPRRRVLEAARGHFLSHGFR
                     TVTMDDLAAELGMSKKTLYAHFPGKEALLEAVMKDKFARVDATLQTIERSRPDDFAAL
                     LRAMLEGMLRELSELKPPFVRDMRLKAPHIFQRLEQRRARLIEKHFRGLFVAGQRSGD
                     VRRDVSVTLMIETILAAIQAIMNPQKLEELSLSPKEAFAGVIGIVLRGVLRPTKGKR"
BASE COUNT          812 a         1246 c         1382 g          820 t
ORIGIN      
        1 gactcgagca tgagccgctc cggcgcgacg tagaggaggc ggaactcgcc gttgtagagt
       61 ccgcgcagcc gcgagcgcgc ctgcgcgcca tcgagcgcgg aattcaaaaa cgtcgctgcg
      121 accccgctcg cctgaagcgc atcgacctgg tccttcatca gcgagatgag cggcgaaatc
      181 acgatcgtca ggccctcgcg caacagcgcc ggcaattgaa aacatagcga cttcccgccg
      241 cccgtcggca tgagggcaaa gacatcgcgt cccgccagtg cgtcgcgcac gatctcctcc
      301 tgcaacggcc gaaaggccga atagccgaag tgtttcttga gggcggcgcg cagttccaaa
      361 gtccgtcagc ttttacttca gcagccgccg gggcaagaac ggaacgctaa tcgagcgtct
      421 gccggtagcg tttgactccg atgatgaggg cgatgagcgc gaaaagcgcg atctgccaga
      481 gctgcaggag gacgtcgttg aaggcgtttc ctttcaacaa gatcccgcgc acgatgcgca
      541 gaaaatgggt gagcgggaaa aattcgccga tcgcctgggc ccacggcggc atgccgcgaa
      601 atgggaacat gaatccggag agcaggatcg agggcagaaa gacgaagaag gtcatctgca
      661 tcgcctgcag ctgatttttc gcgatggtcg aaaaggtaat gcccatggcg agattcgcgg
      721 cgatgaagac gagcgcgact gcgaggagga gaggaatgct gccttgcatc gggacatgaa
      781 aaagaaaatg ggccgcgacc agaatgaccc cgacctggat gtagccgatc atgatgtagg
      841 ggacgatttt tccgatcagc acttcgctcg ggcgggtcgg catggagaga agattttcca
      901 tcgtgccgcg ctcccgctcg cgggtgatcg cgaggccggt gatcatgatc atggtcatgg
      961 tcaggatgac gcccatcagg ccggggacga tgttgtattg ggtgatgatc tccgggttgt
     1021 agagcgcgtg gatcctgacg tcgaccgggc cggcggtggc gttgagcgag gcgagcgagc
     1081 ctttcagatc ctgttgcagc gcggtattga tgacattgcc taacgaggcg agggcgttgc
     1141 cggttgccac cggatcgctc gcgtcggctt ccacgaggat ggcgggttga tcgccgcgca
     1201 gcagatcgcg cgtgaaattc tgcggaatgt tgatcacgaa ctgcacttcg ccgcgcgcca
     1261 ggagattgtg ggcttcctcc tccgtcgcga cctgctcgac gaaattgaag taggtgctgt
     1321 tcttcatcgc ataaagcaac gtgcgcccgt aggggccgcg atcggcgagc acgacagcgg
     1381 cggggagatg gcgcggatcg gagttgatcg cgtaaccgaa aagggtgagc tggatcagcg
     1441 gaacgccgag catcatcgcg aaggtcagcc ggtcgcgccg catctgcgtg aactccttca
     1501 cgatgatccc gcgcaggcgt ccgaaggaga atttagggtg acgcttcatg gcgtgaaatt
     1561 gtccctggaa ccttccatca aactgatgaa ggcgtcctcc agcccggaag gaatctcgcg
     1621 ccaatcgtag ggcggatggc ggaagggggc gatcgcctgc ttgagcgcag ccgcgtcgtc
     1681 gctgcttacg tgcagagcgt tgccaaaggc gaccgcctgc tgcacgcccg acagagcgcg
     1741 gagctgttcg gccaaatgcg ggaggtcagg tccgctgacc gaccacgtcg ttaggccgac
     1801 ttgcgtgatg acatcatcga ccgagccgtc ggccagcagt ttgccgaagg aaatgtaggc
     1861 cagccgatgg cagcgctcgg cttcgtccat gtaatgcgtc gtgatgagga aggtcagccc
     1921 ctgggccgag agctggtgga tttgttccca gaaatcgcgc cgcgctttcg ggtcgacgcc
     1981 cgcggtcggc tcgtcgagca ggagcagctt cggtttgtga atcaggcagg cggcgagcgc
     2041 gaggcgctgt ttccagccgc cggaaagctg gcccgcgagc tgtttttgcc ggccggcgag
     2101 cccgagctgc gcgatgcttt ctttcacggt ggcgcgccga tttttcaccg catacatgcg
     2161 ggcgacgaaa tccagattct cctcgatgct caggtcttcc caaaaactga aacgctgcgt
     2221 catgtaaccg acttcgcgtt tgatcgcttc gctctccgcg atcacatcgt aaccgagcac
     2281 acgtccgctc ccgtcgtcgg cgcggagcaa cccgcaaagc atgcgcagga aagtcgtctt
     2341 gccgctgccg ttcgggccga ggaagccgta gatctgcccc gtgtggacgt gcaaatcgac
     2401 atgatcgacg accgcacgct cgccgaagcg cttcgtcatt ccgcgcacgt cgatggccga
     2461 atcgccgttc attttttcgg ttcgaattgc acatccaccg gctgcccggg gtgcagtttg
     2521 gccgcggtca cgggatcgaa aaccgattcc accatgaaga cgaatttttc ccggctctcc
     2581 tggctgtaga tgaccggcgg ggtgtactcg gcgcgcggcg aaatgtagct gacgcggccg
     2641 ataaacggcg cgctcacccc gtccactgtg acccgcgcgg tgtcgccggg ttgaatcgaa
     2701 ccgatgcgcg tctcgggaac aaaggcgcgc actttgatat tgggcggagg gagcagcgcg
     2761 gcgatcggtt tgccggccgg gacaaattcg ccctgccggt aaagcgtgtc gtaaaccaag
     2821 gcggcctgcg gcgcggattg tttcatctgc gaaaggttcc actccgcttg cgcgagctgc
     2881 tgttggttct ggtcccgaac ggcgcgcgcg ttgtccagat cctgcgccgc cgagggtcct
     2941 tcgtgaaaga gttgctgctg ccgcttgaag gttgcctcgg agagggcgag ggctgctctt
     3001 gcctggtcgc gcgccgccag ttccatcgtc gggtcgagag aaaaaagcgg ctggtcggcg
     3061 gtgacctgct ggccgcgttg cacgtccaga tggtcgagaa taccgccgag cggtgagctg
     3121 acgtagacgt actcaccctc gacgtagccc tggacgcgat cgctcggcgc cttccggcaa
     3181 ccggcgagga gcagaacccc actcgttagg caaacaacgc ggtgcaagat tcggtttgtc
     3241 ttcatcgctt cccttttgtt ggccggagca cgccgcggag cacgatcccg atcacgccgg
     3301 caaaagcctc cttcggtgag aggctcaatt cctcgagctt ctgcggattc atgattgcct
     3361 gaatcgccgc caggatggtc tcgatcatga gggtgacgga gacatcgcgg cgcacatccc
     3421 cagaacgctg gccggcgacg aacaaaccgc gaaaatgctt ttcgatgagc cgcgcgcgtc
     3481 gttgctcgag tcgctggaaa atgtgcggcg ctttcagccg catatcgcgc acgaagggcg
     3541 gcttgagttc actcagctcg cgcagcatcc cctcgagcat cgcgcgcagc agcgcggcga
     3601 aatcatccgg ccgactgcgt tcgatcgtct ggagcgtcgc gtcgacgcgg gcaaatttgt
     3661 ctttcatcac ggcctcgagc agggcctcct tgcccgggaa atgcgcgtag agcgtcttct
     3721 tgctcatgcc cagttcggcg gcgagatcgt ccatcgtcac ggtgcggaaa ccatgactga
     3781 gaaaatgtcc gcgggctgct tccagtaccc ggcggcgcgg ggagagggcg ctgggcgggg
     3841 ccgacctggc ggccgcgatc gacgtcgagc gtgctgttcg cttgtttgtt ttcatttcct
     3901 gcggcgttct gtcgtgtccc tcagagactt cggaaaccaa aagagtgtaa caagtttcct
     3961 agcatcggca aatgaaaatt gttttggcgg tcgcggcgat tccgtcgtgc ccgatcgcgc
     4021 tcctggcagg catctctccc gagggaagag cagtcgggtc tagcccatgc ctacagtccg
     4081 cgcggtctga gcggcgttcg tgcagatttt gaacaacgcc gggtgctcga agattattgt
     4141 gttggccaat gcttctacgg ccgcggcggt gtcctcgcct tcgggcaatt ctgggaagaa
     4201 aaaaaccgcg atgcgttagt cctcgataat tttttccagc tgcgccgccg ggtcccagcc
//
LOCUS       DATMNS010000040        11763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2556962, whole genome shotgun sequence.
ACCESSION   DATMNS010000040 DATMNS010000000
VERSION     DATMNS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 11763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11763
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2556962"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VNW28_01515"
     CDS             <1..616
                     /locus_tag="VNW28_01515"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HXA08627.1"
                     /translation="SGAEFTLLEGAHRCHAVLPVPGLHMVQNALLAVAAGRVFGLSLE
                     ECAAGLAAAPVTQARLQIKEIHGVQFIDDSYNANPDSTKAALRTLAELETAGRRFAVL
                     GEMAELGAEAQRGHEEVGQVAASLGIDCLIALGQGAAEIAAAAESAGLTRSCVVASAA
                     EAAGLLAEAAHPGDLVLVKGSRAARTERVLEEFARRQMETGCAS"
     gene            613..1713
                     /gene="mraY"
                     /locus_tag="VNW28_01520"
     CDS             613..1713
                     /gene="mraY"
                     /locus_tag="VNW28_01520"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXA08628.1"
                     /translation="MMFYLHRLTEQFKGFNVFYYVTFRAVLAAITAFAISLLFGNYII
                     RKLVSLKFGQPIRTASEVRHLAELHGGKKGTPTMGGVLIIGSVVVASVIWARPDNPFV
                     WLVLFCMVYLGVLGFVDDYLKVIKKNPDGISGRLKLLFQILLAGIVTGFFLLNPLIEV
                     QARSLYVPFFKAPVIVNLGMFTFFFFALVIVGASNAVNLTDGLDGLAIGCTITVAFAY
                     ALLAYAAGNFRIAQYLQVPFYSFSGELAVVCAALVGAGLGFLWFNCHPAKVFMGDTGS
                     LAIGGMIGVVAICCKQELLLAVVGGVFVIEAVSVILQVLSFKLTGKRLFAMSPIHHHF
                     ELSGWKENTVIVRFWILSIICALLGLATLKLR"
     gene            1751..3160
                     /gene="murD"
                     /locus_tag="VNW28_01525"
     CDS             1751..3160
                     /gene="murD"
                     /locus_tag="VNW28_01525"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXA08629.1"
                     /translation="MSLRRDALPRVEARLPNERTTFHRPMKPDYKNKKIAVLGAGRSG
                     KAAALLLADEGAKVTLLDSAPEEKLAGLEPLRARGVYLLCGPLAQEDPAVYDLGVFSP
                     GIDPELALAKNFSRKNIPVIGELELGWQFCRVPVIGITGTNGKTTTTELIAQMLNACG
                     QPTVACGNIGKPLVEAARENDDLAVLTVEVSSFQLETIRDFHPEISVWLNFAPDHLDR
                     YRSIREYREAKLRIFENQTAADTAVVNVSENLPPLAPRLTTFSAWTDGGDFSLGNGWI
                     LFRQEPVLQLSDAQLRGPHNAENMMAALAVGRARGLSFAEMVPPLCAYRAQLHRLELI
                     RALGGVDYINDSKATNLDALEKALLSATKPIVLIAGGKDKGFAFDSIAPLVARKVRRA
                     ILIGEIAARVAKDWRDATQCELASSLGAAVERAQAIAHPGETVLFSPGTSSFDMFKSY
                     ADRGDQFRALVRGLPDPPL"
     gene            3157..3711
                     /locus_tag="VNW28_01530"
     CDS             3157..3711
                     /locus_tag="VNW28_01530"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXA08630.1"
                     /translation="MKMPSLLKRKKLRATTARRRTLGLPNEMDYEEMSEPNMKLSRAL
                     LIVLLLHIVAVAGIIAFNTIKTRETTAAGVAESFPSAAPGASAAPSTAPDDATSVAAR
                     SETKTPEKSAEHAKPKTSEVADSGKTYVVVKGDNPVTIARHLHVSYDALLELNQIDDP
                     RKLQIGQKLRIPEHGPATAKHKTD"
     gene            complement(3743..3928)
                     /locus_tag="VNW28_01535"
     CDS             complement(3743..3928)
                     /locus_tag="VNW28_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08631.1"
                     /translation="MHQRLYSLGQSVSEGRASARPVCFGGGDPWLLRPGKNIFPISGP
                     LREKMTDVRKHVPPAAS"
     gene            3923..5065
                     /gene="ftsW"
                     /locus_tag="VNW28_01540"
     CDS             3923..5065
                     /gene="ftsW"
                     /locus_tag="VNW28_01540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HXA08632.1"
                     /translation="MHRKSAYILFLAVLGLLAIGIVILFSTGAFARDSQGDVYFYVRK
                     HALWLGIGLVACLLAALVDYHVWQRTWWIWFALSFVGLALCFLPPFRHKINGSWRWLE
                     FHHFTCQPSEFAKIGAVFFLAFWFSRYEAESKQLWRGFVLPLAVISLLIALIVTEVDL
                     GTTVLIGATTFIVMFIAGSNLALLASLLLGGIGTIFFLATHIAERNSRLMAFLHPEQY
                     KLTAGLQQMQALIAWGSGGIGGLGLGNGRQKMSYLPYAHTDFIFPIIGEELGLRFSLL
                     VIFLFVLIIICGLTIALHARDRFGLLLASGIVSLIAFQAAINIGVVTSLLPNKGLPLP
                     FVSYGGSNLVVALFAVGLLLNIYRQGVLEPPPRKEMAMRVRVTPRI"
     gene            5106..6209
                     /gene="murG"
                     /locus_tag="VNW28_01545"
     CDS             5106..6209
                     /gene="murG"
                     /locus_tag="VNW28_01545"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HXA08633.1"
                     /translation="MNAVIACGGTGGHLFPGLAVAEVLRARGHEVLVFISEKEIDSLA
                     LEGRTDFRFEKLPTIALPSPFSPAILAFVRRFNDSLSLCRGVFRKFNPHVVLGMGGFT
                     STAPVLAGRMRGIPTFIHESNAFPGKANRHTARFVRAVLLGFKECAAFFPKAQTEFTG
                     TPIRSSLRRVDRAGALEKLGLRPGIPTLLVMGGSQGASGINQALIKALPALHGVALQV
                     IHLSGSRDERLLEDNYRRENIPAYVAAFHHRMEEVYSAADFAIARSGAASLSELAAFA
                     LPALLVPYPYAADDHQTRNAEIFVRANAALLVRESELTGEPLAQKIRDLLSNPETLRA
                     MSTHAAALAAKNAASLVVDTMERYSHPNDAVAA"
     gene            6190..8490
                     /gene="murC"
                     /locus_tag="VNW28_01550"
     CDS             6190..8490
                     /gene="murC"
                     /locus_tag="VNW28_01550"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXA08634.1"
                     /translation="MTPSPPDLDLRKFLTEERHAIHLIGVAGSGMSGIAALLLELGHD
                     VRGSDKARTLETERLQQLGLRFYSPHDAADAADAELVVFSSAIKQGNPILSAAREHRT
                     PAIRRAEALAAMMRGKRGIIIAGMHGKTTTAAMAAHVLRGGQLHPSHYVGAEIPILGS
                     NAHWDARGEFFVAEGDESDGTLQLFHPEHTLILNIEEEHLDFYKDLAAIEVVFTKLLQ
                     QTSGVVFYCADDPHAARLCGARSGAVSFGFSERARYRATGLELHDFAATFCVERDAEK
                     LGDATLSVPGRHNVSNALAVIALATELGIPFEKIAKSLGTFRHARRRFEIKYQSDRFL
                     LVDDYAHHPTEIRATLATAKSAGRKRVLTMFQPHRYSRTRALRQEFGAAFADADHVFV
                     TDVYAASEASLPGVTGQTIADAISAHGHRGVSYQPRLDRLHCDMGRMLAAGDLVLSLG
                     AGNVHEQLATLAAELVIAEKLKEIVGAEGDVRLSEPLAKHTTLRVGGPAQFWVEPRSE
                     ETFARLIRFCRRENLPLFVIGRGSNLLVRDGGIRGVVVHPSGGEFDRVEASGLEVTAG
                     VGARLKEVAYAGKAAGIGGFEWMEGIPGAVGGGLKMNAGAMGAQTFDQVVRVRYLDRE
                     GIAHEKTPAELEVHYRHVPALDENFAVSAVFRGEKSSAAEIVRRLEASQEKRRTTQPS
                     AKSAGCIFKNPAACPAGKLVDELGLKGSRVGDARVSEVHGNFIVNEGAATADEVLQLI
                     GQIQATARQKRGIELETEVQIVGEAG"
     gene            8663..9592
                     /locus_tag="VNW28_01555"
     CDS             8663..9592
                     /locus_tag="VNW28_01555"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HXA08635.1"
                     /translation="MIALTGMQIAVLKGGPGSERAVSLATGAGVARALRSLGAEVTEV
                     DVENADFSLPNGTGLAFIALHGTFGEDGQVQQILEDREIPYTGEGVAESRLAFDKIPS
                     KERFTRHGVTTPHWEVIRGGERPTLPLPYVIKAPRQGSTVGVYIVKTESEVAPALREA
                     AHYDDEFLIEQFIPGRELTVGVLGEQALPVLEIIPKSGFYDFNDKYPFLNPQGGGGAQ
                     HVCPAALPGDLTRKIQDLALLACGSLGLKVYSRVDFMLSASDEPFVLEINTIPGMTEA
                     SLLPEAAAAAGISYPQLCLRIIELSLAARPAKQ"
     gene            9589..10716
                     /locus_tag="VNW28_01560"
     CDS             9589..10716
                     /locus_tag="VNW28_01560"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HXA08636.1"
                     /translation="MKAKPKKNQPRAGNRRLSTWRQRKQQHLLDVKVRARRASHHRNR
                     RILVAIAKVALVIAVCAGLYFGARTALSRFFFDNPDYRLSTIAVQTDGTLLRDQILKS
                     AGLHEGLNIFSINLAQVHDRLQQLPQVDDVQVERKMPNEIDIAVTERKPIAWITSEKI
                     VANPFASDAAFLVDARGILMKEKKLLPEYLALPLITGCSSEALVPGKMVDSFEAKAAL
                     ELLRLTTTSFMQTRFQIREVDVSKGYCLLVTDKNHTEVMFGFDHLDTQMQRLEQFLVY
                     SDDAHREIATVNLLVARNIPVTFAKPVSAVISELEPAPKETKPAAPEPEIRKAKAVQP
                     IIRKALPYSPGVQKFHSSPPPLPVRKAIPLQRFNQEGNGNG"
     gene            10709..>11763
                     /gene="ftsA"
                     /locus_tag="VNW28_01565"
     CDS             10709..>11763
                     /gene="ftsA"
                     /locus_tag="VNW28_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXA08637.1"
                     /translation="MASSNLMVGLEIGTSKICVVVGEGKPDGTIKILGVGQAPSRGVR
                     KGEIVDFETAMKCVHEAVVDAEEKSDIMIRSVYIGITGAHIQSFNNRGCVRVSEDRGE
                     IDEQDIEDVKINAREVSIPAQNAFLHSIIQHYSVDGQNGVLNPVGMLGQKLEADFHII
                     HGVRTRIQNTIRCVKELPLDVEDVVFTPFAAAQVVLTQHQKNLGALVIDIGGGTTDYV
                     LYVDGAVKQSGVLAVGGDHITNDISMGLRIPMTRAEKLKLEEGSTVLGNCLPGETVLL
                     KDDSGFAGKEVERETLNTIIHMRLRETFELLKRRLEDEPFLDYLGEGIFITGGCSQLN
                     GIDNLAEDVFGIPARAS"
BASE COUNT         2164 a         3719 c         3623 g         2257 t
ORIGIN      
        1 gagcggcgcg gaattcaccc tcctcgaagg cgcgcatcgc tgccacgcgg tcctgcccgt
       61 gcccggtctg cacatggtgc agaacgcgct cctcgccgtt gccgccgggc gcgtctttgg
      121 cctttccctc gaggaatgcg ccgccggtct ggcggccgcg cccgtcaccc aggctcgttt
      181 gcagatcaag gaaattcacg gggtccagtt catcgacgat agctacaacg ccaatccgga
      241 ctcaaccaag gccgcgctcc gcaccctcgc cgaactcgag accgctggcc ggcgtttcgc
      301 cgtcctcgga gagatggcgg agctcggggc cgaagcgcag cggggccacg aggaggtcgg
      361 tcaggtcgcg gcgtcgttag gcatcgattg cctgatcgct ctcggccagg gcgccgcgga
      421 gatcgccgcg gccgcggaga gcgccggcct cacgagaagc tgcgtggtcg cgtcggcggc
      481 ggaagcggcc gggctgctcg cggaggcggc gcacccgggc gatctcgtgc tcgtcaaggg
      541 cagccgcgcg gcccggaccg aacgcgtgct ggaggaattc gcgaggcgcc agatggagac
      601 cggctgcgcg tcatgatgtt ctacctgcat cgcctgacgg agcagttcaa gggcttcaac
      661 gttttctact atgtcacctt ccgcgccgtc cttgcggcca tcaccgcttt tgcgatttcg
      721 ctcctctttg gcaactacat cattcggaaa ttggtttcgc tgaagtttgg gcagccgatt
      781 cgcaccgcct cggaagtgcg gcatctggcc gagctgcacg gcggcaaaaa aggcacgccc
      841 accatgggcg gtgtgctcat catcggttcc gtcgtcgtcg cctccgtcat ctgggcgcgg
      901 ccggacaacc cattcgtctg gctcgtgctt ttctgcatgg tctatctcgg cgtcctcggg
      961 ttcgtggatg actacctaaa ggtcatcaag aaaaatcccg atggcatcag tggccggctc
     1021 aaattgctct tccaaatttt gctcgccggg atcgtgaccg gattttttct gctcaatccg
     1081 ctgatcgaag tgcaggcgcg ctcgctctac gtgccgtttt tcaaggcgcc ggtgatcgtc
     1141 aacctgggga tgttcacttt tttctttttc gcgctcgtca tcgtcggtgc gtccaacgcg
     1201 gtcaatctca ctgatggtct cgacggcctg gcgattggct gcacgatcac agtcgcgttc
     1261 gcctacgcgc tgctcgccta cgcggccggc aattttcgca tcgcgcaata tctccaggtc
     1321 ccgttttact ccttctcggg cgagctcgcc gttgtctgcg cggcgctcgt cggcgccggc
     1381 ctgggtttcc tctggttcaa ctgccatccg gccaaagttt tcatgggcga caccggctcg
     1441 ctcgcgatcg gtggaatgat cggtgtcgtc gcgatttgtt gtaaacagga gctgctcctg
     1501 gcggtcgtgg gcggtgtttt tgtgatcgag gccgtctccg tcattctgca agtgctgagc
     1561 tttaagttga ccggcaaacg gctcttcgcc atgtcgccga ttcatcatca tttcgaactg
     1621 agcggctgga aggaaaacac cgtcatcgtg cggttctgga ttctctccat catctgcgcg
     1681 ctcctcggcc tcgccacctt gaaactacga tgaaggcatt tttttcatct gagcctggac
     1741 aggcggaagc atgtccctcc ggagggacgc gcttccgcgc gtcgaggccc gtctgcctaa
     1801 cgaacgcacg acgttccacc gcccgatgaa gcccgattac aaaaacaaaa aaatcgccgt
     1861 tctcggcgcg ggccggagcg gaaaggcggc ggcgcttttg ctcgcggacg aaggcgccaa
     1921 agtgacgctg ctcgacagcg cgcccgagga aaaactggcc ggtctcgagc cgctgcgggc
     1981 gcgcggcgtt tacctgctct gcggtccgct cgcgcaggag gatcccgcgg tctacgacct
     2041 cggcgttttt agcccgggga tcgatccgga actcgctctg gccaaaaatt tttcgcgcaa
     2101 gaacatcccg gtgatcgggg agttggaact cggctggcag ttctgtcgag tgccggtcat
     2161 cgggatcacc ggcacaaacg gcaagacgac gacgacggaa ctgatcgcgc aaatgctcaa
     2221 cgcctgcggc cagccgacgg tcgcctgcgg gaatatcggc aagccgctcg ttgaggcggc
     2281 gcgcgagaac gacgatctcg ccgtcctgac cgtcgaagtg agctcgtttc agctcgagac
     2341 gatccgcgat tttcatccgg agatcagcgt ctggctcaac ttcgcgcccg accatctcga
     2401 tcgctaccgc tcgatccgcg aataccgcga ggcgaagctg cgcatcttcg agaaccagac
     2461 cgccgccgat accgcggtgg tcaacgtttc ggaaaatctc ccaccgctcg cgcctcggct
     2521 cacgaccttc agcgcctgga ccgacggcgg cgacttttcg ttaggcaacg gctggattct
     2581 tttccggcag gagccggtgc tgcaactttc cgacgcgcag ttgcgcggac cgcataatgc
     2641 cgaaaacatg atggcggcac tcgccgtcgg ccgggcccgg ggcctttcct tcgcggagat
     2701 ggtcccgccg ttgtgcgcct atcgcgcgca gttgcatcgc ctggaattga tccgcgcgct
     2761 cggcggggtc gattacatca acgattcgaa ggcgaccaat ctcgatgcgc tcgaaaaggc
     2821 gctcctcagc gcgacgaaac cgatcgtgct catcgcgggc gggaaggaca agggtttcgc
     2881 cttcgattcg atcgcgccgc tcgtcgcccg caaggtgcgc cgcgcgattc taatcggcga
     2941 gatcgcggcg cgggtggcga aggactggcg cgacgcgacg caatgcgagc tcgcgtcttc
     3001 gttaggcgcg gccgtcgagc gcgcgcaggc catcgcgcat ccgggcgaga cggtcctctt
     3061 ttctcccggc acctcctctt tcgacatgtt caaaagctac gccgaccgcg gcgaccaatt
     3121 ccgcgccctc gtgcgcgggc tacccgaccc accactatga aaatgccatc gctcctgaaa
     3181 cgaaagaaac tccgcgccac gacggcccgg cgtcgcacgc tcggcctgcc caacgaaatg
     3241 gactacgagg agatgtcgga gccgaacatg aaattgtcgc gcgccctcct catcgtgctt
     3301 cttctccaca tcgtggccgt cgccgggatc atcgctttca acacgatcaa gacccgggag
     3361 acaaccgcgg cgggggtcgc ggaatcgttt ccctccgccg cgccgggggc gagtgccgcg
     3421 ccgtcgaccg cgcctgacga tgccacgtcg gtggcggcgc ggtccgagac caagacgccg
     3481 gagaagagcg cggagcacgc caagccgaag acctccgagg tggcggattc agggaagacc
     3541 tatgtcgtgg tcaaaggcga caacccggtg acgatcgcgc gccatctgca cgtctcctac
     3601 gacgccctgc tcgagctgaa ccagatcgat gacccacgca agctgcagat cgggcagaag
     3661 ctgcgcattc ccgagcacgg cccggcaacc gccaaacaca aaacagacta gaatggcgca
     3721 aacgttcgga tttttcagtg ggctaggaag ctgccggagg gacgtgcttc cgcacgtccg
     3781 tcatcttttc ccggagcggg cccgagattg gaaagatgtt tttccccggc cgcaagagcc
     3841 agggatcgcc gccgccaaag caaaccggac gtgcggaagc acgtccctcc gagacggact
     3901 ggcctaacga gtataaccgc tgatgcatcg caagagcgcc tacatcttat tcctcgctgt
     3961 cctgggattg ctcgccatcg gtatcgtcat ccttttcagc accggcgctt ttgcccggga
     4021 cagccagggc gatgtctatt tctacgtgcg aaagcatgct ctctggctcg ggattggttt
     4081 ggtcgcctgt cttctggcgg ccctggtcga ttaccacgtc tggcagcgca cctggtggat
     4141 ctggttcgcg ctctccttcg ttggcctggc gctttgtttt ctcccgcctt ttcgtcacaa
     4201 gatcaacggc tcctggcgct ggctggagtt tcaccacttc acctgtcagc cctcggagtt
     4261 cgcgaaaatc ggcgccgtct tcttcctcgc cttctggttc tcgcgctacg aggccgagag
     4321 caagcaactc tggcgcggtt tcgtgctccc gctcgccgtc atcagtctgc tcattgcctt
     4381 gatcgtgacc gaggtcgatc tcggcacgac ggtcctgatc ggcgccacca ctttcattgt
     4441 catgttcatc gccggcagca acctcgcgct cctcgccagt ctgttgttag gcgggatcgg
     4501 cacgattttt ttcctcgcca cgcacatcgc cgaacggaac agccggctga tggccttcct
     4561 tcatccggag caatacaaac tgacggccgg attgcagcag atgcaggccc tgattgcctg
     4621 gggcagcggc gggatcggcg ggctcggcct cggcaacggc cggcaaaaaa tgtcctatct
     4681 gccctacgcg cacaccgatt ttatttttcc catcatcggc gaggaactcg ggctgcgttt
     4741 cagcctgctc gttatttttc ttttcgtcct cattatcatc tgtggtctga cgatcgcgct
     4801 ccacgcgcgg gatcgatttg gactgcttct cgcgagcgga atcgtttccc tgatcgcctt
     4861 ccaggccgcg atcaacatcg gagtggtgac ctcgcttttg ccgaacaagg ggttgccgct
     4921 gccctttgtg agctatggcg gctcgaatct tgtcgtggcg ctttttgcgg ttgggcttct
     4981 cttaaatatc taccgacagg gcgtgctcga gccgccgcca cgcaaggaaa tggcgatgcg
     5041 ggtccgggtc accccgagga tctaatgcgg ctctcaactc ccaattctca actctcaacc
     5101 tctgcatgaa cgccgtgatc gcatgcggag gaacaggcgg acatcttttc cccggcctcg
     5161 cggtggccga agtgctgcgg gcgcgcgggc acgaggtgct cgtctttatc tccgagaagg
     5221 agatcgacag cctcgcgctc gaagggcgaa cggattttcg ctttgaaaaa ctcccgacca
     5281 tcgcactgcc ctcgcctttc tcgcccgcca ttctcgcctt cgtgaggcga ttcaacgaca
     5341 gtctttcgct ctgccgcggg gttttccgca agttcaatcc gcatgtcgtc ctcgggatgg
     5401 gcggtttcac ttccacggcg ccggtcctcg ccggacggat gcgcggcatc ccgaccttca
     5461 tccacgagtc gaatgccttt cccgggaaag cgaatcgtca caccgcgcgt tttgttcgcg
     5521 ccgttcttct cggtttcaag gagtgcgcgg cgtttttccc gaaggcgcag acggaattta
     5581 ccggcacgcc gattcgctcc agcctgcggc gggtcgatcg cgcgggggcg ctcgaaaaac
     5641 tcgggctgcg cccgggaatc ccgaccctcc tcgtgatggg cggcagccag ggggcgagcg
     5701 gcatcaacca ggcgttgatc aaggcactgc ccgcgctgca cggcgtcgcg ctccaggtga
     5761 ttcacctctc cggcagtcgc gacgagcgtt tgctggagga taattatcga cgcgaaaata
     5821 tccccgccta tgtcgccgct tttcatcacc ggatggagga agtctacagt gcggcggatt
     5881 ttgccatcgc gcgctccggc gcggccagtc tctccgagct cgcggccttc gcgctgcccg
     5941 ctctcctcgt accctatccc tacgcggcgg acgatcacca gacgcgcaat gccgagatct
     6001 tcgtccgggc aaacgccgcc ctcctggtca gggaaagcga attaaccggc gaaccgctcg
     6061 cgcaaaaaat tcgcgattta ttgagcaacc cggaaaccct ccgcgccatg tcgacccacg
     6121 ccgcggcgct ggcggcaaaa aacgcggcca gtttggtcgt cgacacgatg gagagataca
     6181 gtcacccaaa tgacgccgtc gccgcctgat ctcgaccttc ggaaattcct caccgaggag
     6241 cgacacgcga tccatttgat cggcgtcgcg ggcagcggga tgagcgggat tgccgcgctc
     6301 ctgctcgagc tcgggcacga tgtgcgcggg tccgacaagg cacgcaccct cgagaccgag
     6361 cggctgcagc aactcgggct acgcttttat tctccgcacg acgcggcgga cgcagccgac
     6421 gcggagctgg tcgttttctc gtcggcgatc aagcagggca atcccattct gagcgcggcg
     6481 cgggagcacc ggacgccggc gattcggcgg gctgaagcgc tggccgcgat gatgcgcgga
     6541 aaacgcggga tcatcattgc ggggatgcac ggcaaaacga cgaccgccgc gatggcggca
     6601 cacgttttgc gcgggggcca gttgcacccg tcgcattatg tcggcgcaga gattccgatt
     6661 ctcgggagca acgcgcactg ggatgcgcgc ggcgaattct tcgtcgcgga aggcgatgag
     6721 agcgacggca cgctgcagct ttttcacccg gagcacacgc tgattctcaa catcgaagaa
     6781 gagcatctcg acttctacaa ggacctcgcc gcgatcgagg tcgtgttcac gaaactgctc
     6841 cagcaaacca gcggcgtggt tttttattgt gcggacgatc cgcacgccgc gcgtctttgc
     6901 ggggcgcgga gcggggccgt ttccttcggg ttcagcgagc gggcgcgcta tcgcgcgacc
     6961 ggcctcgagc tgcacgattt cgcggcgacg ttttgtgtgg aacgcgacgc ggaaaagctg
     7021 ggcgatgcca ccctgagcgt gccgggcagg cacaacgtga gcaacgctct cgccgttatt
     7081 gcgctcgcca ccgagctggg gattccgttc gagaaaatcg ccaaatcgtt aggcactttc
     7141 cggcacgccc gccgccgctt cgagatcaag tatcagagcg atcgttttct cctggtcgac
     7201 gattacgccc atcatccgac cgagattcgc gccaccctcg cgactgcgaa atccgccggg
     7261 cgcaaacgcg tcctgacgat gtttcagccg catcgttatt cccgcaccag ggcgttgcgg
     7321 caggagtttg gcgcggcctt tgcagacgcg gatcacgttt tcgtgaccga cgtctacgcc
     7381 gcgagcgagg cctcgttgcc gggcgtcacc gggcaaacga ttgcggacgc gatttcagcg
     7441 cacggtcatc gcggggtctc gtatcaaccg cggctcgatc ggttgcattg cgacatgggc
     7501 cgcatgctcg ccgcaggcga cctcgtgctc agtctcggcg ccggtaatgt gcacgagcag
     7561 ttggccacgc tcgccgcgga actcgtcatc gcggaaaagc tgaaggaaat cgtcggcgcg
     7621 gaaggcgacg tcaggctgtc cgagccgctg gcgaaacata ctaccctgcg ggtcggcggg
     7681 ccggcgcagt tctgggtgga gccgcgcagc gaggaaactt ttgccaggct gattcgattc
     7741 tgccggcgcg agaatctgcc gctcttcgtc atcgggcgcg gctcgaatct gctcgtgcgc
     7801 gatggtggta ttcgcggcgt cgtcgtgcac ccgagcggcg gtgagttcga ccgggtggaa
     7861 gcgtccggcc tcgaagtcac cgcaggcgtg ggcgcgagac tgaaggaagt cgcttacgcg
     7921 ggaaaagccg ccggcatcgg cgggttcgaa tggatggaag gaatcccggg cgcggtcggt
     7981 ggcgggttga agatgaacgc gggcgcgatg ggcgcgcaga ccttcgatca ggtggtgcgg
     8041 gtgcgttacc tcgatcggga agggatcgcg cacgagaaga cgccggcgga actggaagtg
     8101 cattaccggc acgttcccgc gctcgacgaa aactttgccg tctccgcggt cttccgcggg
     8161 gagaaatcgt ctgccgccga aatcgttagg cgactggagg cttcgcagga gaaacggcgc
     8221 accacgcaac cgagcgcgaa gagtgccggc tgcatcttca agaatccggc ggcctgcccg
     8281 gcgggcaaac tggtcgacga gctgggcttg aaaggttcgc gggtggggga tgcgcgggtc
     8341 tccgaggtgc acggcaactt tatcgtgaac gaaggcgcgg cgaccgcgga cgaagtgttg
     8401 cagttaatcg ggcaaatcca ggcaacggcg cgccagaaac gcgggatcga actcgaaacc
     8461 gaagtgcaaa tcgtggggga ggcaggatga accgcgatcg cagcttccgg ggagcgcacg
     8521 cgcctcgcgt gcccaatccg gcgactcgcc ggattgcccg agcggaaacg agacatgaaa
     8581 actatcccgc ggtcccgccc gcagaagttg gcggcgagtc gccgccaacg gcacgcgagt
     8641 cgcgtgcgct ccccggaaat ctatgatcgc gctcactgga atgcagatcg ccgttctgaa
     8701 gggcggcccg ggctccgagc gcgcggtttc gcttgcgacc ggcgccggcg tcgcccgggc
     8761 gctccgttcg ttaggcgcgg aagtcacgga agtcgatgtc gagaacgccg acttctcttt
     8821 gccgaacggg accggactgg ccttcatcgc tctccacggc acatttggcg aggacggtca
     8881 ggtgcagcaa atcctggaag atcgagaaat tccctacacc ggcgaaggcg tggccgagag
     8941 ccgcctcgcc ttcgacaaga ttccctcgaa ggaaagattt acccggcatg gcgtgaccac
     9001 gccgcattgg gaagtcatcc gcggcggcga acggccgacg cttccgctgc cctacgtgat
     9061 caaggcgccg cgccagggat cgacggtcgg tgtttatatt gtgaaaaccg agagcgaggt
     9121 cgcccccgcg ctgcgcgagg ccgcgcatta cgacgacgag tttttgatcg agcaatttat
     9181 tcccggccgc gagctcaccg tcggcgtgct tggcgaacag gcgctgcccg tgctcgagat
     9241 cattcccaag agcggctttt acgatttcaa cgacaagtat ccgtttctca atccgcaggg
     9301 tggcggcggc gcgcagcatg tttgcccggc cgcgcttccg ggcgacctga cgagaaaaat
     9361 ccaggatctc gccttgcttg cctgtggttc gttaggcttg aaggtttatt cgcgggtcga
     9421 tttcatgctc tcggcgagcg atgaaccgtt tgtcctcgag atcaacacca tcccgggcat
     9481 gactgaggca agcctgctcc cggaggcggc ggccgccgcc ggcatcagct atccgcaact
     9541 ctgtctccgg atcatcgagc tttcactcgc cgcgcggccg gccaaacaat gaaagcgaag
     9601 ccgaagaaaa atcagccgcg cgccgggaac cggcggctct cgacgtggcg ccagcgcaag
     9661 cagcaacacc tgctcgacgt caaggtgcgg gcgcgccgcg cgtcgcatca tcgcaaccgc
     9721 cgcatcctcg tcgccatcgc caaggtggcg ctggtgatcg cggtttgcgc cgggctgtat
     9781 tttggcgcgc gcaccgcatt gagtcgcttc tttttcgaca atcccgacta ccggttgagc
     9841 acgatcgcgg ttcaaaccga cgggacgctg ctgcgcgatc agattctgaa atccgctggg
     9901 ttgcacgagg gattgaacat cttttccatc aacctcgcgc aggtccacga ccggctgcag
     9961 cagctcccgc aggtcgatga cgtgcaggtt gagcgcaaga tgcctaacga gatcgatatc
    10021 gcggtcacgg aacgcaaacc gatcgcctgg atcacgagcg agaaaattgt cgccaatccc
    10081 ttcgcttccg acgcggcctt tctcgtcgac gcgcgcggga tcctgatgaa ggaaaagaaa
    10141 ctcctccccg aatatctggc gctgccgctg atcaccggct gctcgagcga ggccctcgtg
    10201 ccgggcaaga tggtcgattc attcgaggcg aaagcggccc tcgaactcct tcgcctaacg
    10261 accaccagtt tcatgcaaac ccgctttcag atccgggaag tggatgtctc gaaaggctat
    10321 tgcctgctcg tgacggacaa gaatcacacc gaggtgatgt tcgggttcga ccatctcgac
    10381 acccagatgc agcggctcga gcagttcctc gtctacagcg acgatgcgca tcgcgaaatc
    10441 gcgaccgtca acctgcttgt cgcgcgcaat atcccggtga cctttgccaa accggtctca
    10501 gccgtcatct cggaactcga accggcgccg aaggaaacca agccggctgc cccggagccg
    10561 gaaatccgca aggcaaaagc ggtgcaacca atcatcagaa aggcgctgcc ctacagcccc
    10621 ggtgtgcaaa aattccactc cagtcctccg cctcttccgg tgcggaaggc gatcccactt
    10681 cagcgtttta accaggaagg caacggtaat ggctagcagc aacttgatgg tcggtctcga
    10741 aatcggaacc tcaaaaattt gcgtcgtcgt gggcgaagga aaacccgacg gcacgatcaa
    10801 aattctcggc gtcgggcagg cgccgtcgcg cggggtgcgc aaaggcgaga tcgtcgattt
    10861 cgagacggcc atgaaatgcg tgcacgaggc ggtcgtcgat gccgaggaga agagcgacat
    10921 catgattcgc agcgtctaca tcggcatcac tggcgcgcac atccagagct ttaacaatcg
    10981 cggctgtgtc cgcgtctcgg aggatcgcgg ggagatcgac gagcaggata tcgaagatgt
    11041 aaaaatcaac gcgcgcgaag tcagcatccc ggcgcagaac gcctttctgc actcgatcat
    11101 ccagcattat tctgtcgacg ggcagaacgg cgtgctcaat ccggtcggca tgctcggcca
    11161 gaaactggag gccgatttcc acatcatcca cggcgtgcgc acgcggatcc aaaacacgat
    11221 tcgttgcgtc aaggaattgc cgctcgatgt cgaggatgtc gtcttcacgc ccttcgcggc
    11281 cgcccaggtc gtcctcacgc agcatcagaa aaatctcggc gcgctcgtca tcgacatcgg
    11341 aggcgggacg accgattacg tgctctacgt cgatggcgcg gtgaaacaaa gcggcgtgct
    11401 cgcggtcggc ggcgatcaca tcacgaacga catttcgatg ggattgcgca tcccgatgac
    11461 gcgcgcggaa aagctcaagc tcgaggaggg gagcacggtg ctcggcaatt gtctcccggg
    11521 cgagacggtt ctgctcaagg acgactccgg tttcgccggg aaggaagtcg agcgcgagac
    11581 cttgaacacg atcatccaca tgcgcctgcg cgagactttc gaactcctca agcgccggct
    11641 cgaggacgag ccgtttctcg attatctcgg cgaaggcatt ttcattaccg gcggctgtag
    11701 ccagctcaac ggcatcgata acctggccga agacgttttt ggaattccgg cccgcgcctc
    11761 gca
//
LOCUS       DATMNS010000041         7211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2890765, whole genome shotgun sequence.
ACCESSION   DATMNS010000041 DATMNS010000000
VERSION     DATMNS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2890765"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VNW28_01570"
     CDS             <1..654
                     /locus_tag="VNW28_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXA08638.1"
                     /translation="AGIVLGFAAQKTLGNLLAGIQIALSQPILIDDVVIVEGEFGRIE
                     EITLTFVTVRTWDLRRLVVPITYFIEKPFQNWSRKSTEILGAIFLYLDYQVPLGELRA
                     EVKRLVDGNEKWDRQVCGLQVTDAKERTLEVRVVVSSTNASKNFDLRCEVREGLIEFL
                     RTKYPESLPRTRLTLQRHEETGQLPAQYGMAVEGKEHERGSTAPGLEPKDSDGAARR"
     gene            754..1575
                     /locus_tag="VNW28_01575"
     CDS             754..1575
                     /locus_tag="VNW28_01575"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HXA08639.1"
                     /translation="MFQKDAIVRLLRDNDPATVTLLKAQLIDSGMEGAADLRDLLALD
                     DAAVTAHVREVIEKIETDEAQDEMLLCAHLFPEHGDIEAAWWLLTLCFEPDAPIAKLR
                     RRLDAWGRRLRMLVAKAGSARERLQALTTFLAEDLGFRGNAEEYYDPKNSLLSDVIET
                     RLGIPISLAMLYVIVGRRANMQIDGINLPGHFIARYDRILFDPFHQGRILSRPDCEAI
                     LARQRLKTESSYFAPASPRLVLMRMLANLLFIFERAGEKQKHQLVMNWLKALDRD"
     gene            complement(1650..3068)
                     /locus_tag="VNW28_01580"
     CDS             complement(1650..3068)
                     /locus_tag="VNW28_01580"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HXA08640.1"
                     /translation="MPRSSKPAVLAIDIGTSSVRTALFDERAQRVPGSAASQVYRVRH
                     SAEHGAELDPTVLWQATRKCLSQTRGLTKSPVRAIGGSGFWHSLLGLDRAGEPLTPIY
                     TWADARCAEDAARLRERLEERAIQQRTGCMLRASFWPAKLLWLRRTQPRLFRRVTRWV
                     SPAEWIFEKAFGARGCSHSMASGTGLYNFARRDWDKDLLEISGLTTSRLGPLRDAVRL
                     DGVTVFPAIGDGAASNLGSGASRPGIVAINIGTSAAVRAIPAANARLPFGLFRFVVDQ
                     KRALLGGAVSNAGNLRAWCRRELRLPDDERALEKALRQPSAATARLTILPFWVSERAP
                     SWPEHLDGAILGVTLASTAVDLLRATTAAVYHRLAQILEQLEGAIGPAKRVIVSGGIL
                     QSPASLQILADSLGHDLETCLDQEASLRGAAVHVLEQLGGRIPALKRGQVFRHDRAKA
                     NNARQARARQIQLEELFQSRGR"
     gene            3178..3603
                     /locus_tag="VNW28_01585"
     CDS             3178..3603
                     /locus_tag="VNW28_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXA08641.1"
                     /translation="MYKKILVALDNSSTDQAMFRHIAELAKVHAAELLLVHVADGWAA
                     RNYNRFQLADSEEMKEDRAYLEKEAADLRAQGLKVQTQLALGDPATEILKASTEEKCD
                     LIAMTTHGHRFFKDLLYGSTITQVRHRARMPVLLVNVAK"
     gene            3640..4845
                     /gene="glgA"
                     /locus_tag="VNW28_01590"
     CDS             3640..4845
                     /gene="glgA"
                     /locus_tag="VNW28_01590"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HXA08642.1"
                     /translation="MKTLFLTNEYPPHIYGGAGVHVEYLSRELAKLMEVEVRCFGDQQ
                     SLGTNPEVRGFELATSSYTCPPRLQSVFGAVQRCLDFNTLNIAADVVHCHTWYSHFGG
                     ILAKVNYGIPLVITVHSLEPLRPWKREQLGGGYDFTVWLERTALEMADAVIAVSNGTK
                     ADVARLFQIKPERLHVIYNGIDLDEYRPTDATDALVRHGVDPAQPYLLFVGRITRQKG
                     IIHLVHAIQFMDPDFQIVLCAGAPDTPEIAAEMKAVVGEAQAKRNGIIWIDEMVDRET
                     VHQLYAQAAVFCCPSIYEPFGIINLEAMACETAVVASAVGGIKEVVVDDETGFLVPVE
                     QMLESPFEAIDPERFERDLAARVNQLMADPVLQRKFGRAGRRRAEEKFGWAAIARETK
                     HLYESLLKK"
     gene            4888..6045
                     /gene="obgE"
                     /locus_tag="VNW28_01595"
     CDS             4888..6045
                     /gene="obgE"
                     /locus_tag="VNW28_01595"
                     /inference="COORDINATES: protein motif:HMM:NF008955.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HXA08643.1"
                     /translation="MRGTVYRVTMFVDRIKVVAKGGDGGRGCVSFRREKFVPRGGPDG
                     GDGGKGGDVILRADEHTDNLASLFYEPLIKAKNGAHGMGKQMHGRGALAKVVKVPVGT
                     VVHRIDSLGEAAAFTDAAGAAEVLAVPGATLEQIADLALPNEEFVLCHGGKGGKGNVH
                     FKSARNRAPRQYSDGEEGEEGTFVFELRTIADAGLVGYPNAGKSTLLRRISAAHPKVA
                     SYPFTTLHPMIGVVEFADYRRATLADIPGLIEGAHRNLGLGHAFLRHITRCRLLLFVL
                     DMAGSEGRNPIEDLQHLRREIDLYDPRLSLRPWRVVANKMDLPEAAENLAAFRERFPE
                     REVIPVSAKENEGIENLKRALDRWLNEPELELPGPRAEGYTIRVRQAEAMR"
     gene            6093..>7211
                     /locus_tag="VNW28_01600"
     CDS             6093..>7211
                     /locus_tag="VNW28_01600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA08644.1"
                     /translation="MSKLVVASYCTTFLKSEMLHIYRQVTALRDVQTFVMTKKVQNND
                     RFPFDDIELIPAPRPNPFRHGWLKFVERQPPIVYRGEYQLLANLLEHRGADLMHIYFG
                     HTAVHLLPFIECWNKPCIVSFHGADVMVKAESPDYAAKLRRVFQSVPLVLARSRSLEQ
                     RLIVLGCPPEKIRINRTGIPLEQFPLVRRQAPEGGRWRFLQACRLIPKKGLTTCLRAF
                     AVFRRENPGAELLIAGKGPLQPVLEALAAQLDISDHVHFTGFLSQPDLLALYHSCHVF
                     LHPSEMPADENQEGIPNSILEAMATGMPVLATRHGGIPEAVEEGRCGALVEERDYLAL
                     AAEMKKITRSPHSFGEIGVLASESVAAHFEQRVHIAQLE"
BASE COUNT         1420 a         2053 c         2361 g         1377 t
ORIGIN      
        1 gcgggcatcg tcctcggttt cgcggcgcaa aagacgctcg gaaatctgct cgccggaatc
       61 cagatcgcgc tcagccagcc gatcctgatc gacgacgtcg tgatcgtgga aggcgagttc
      121 ggccggatcg aggaaatcac ccttaccttt gtcacggtgc ggacgtggga cctgcggcgg
      181 ctggtcgtgc cgattaccta ttttatcgag aagccgttcc agaactggag ccgcaaatcg
      241 accgaaattc tgggcgcgat ttttctttat ctcgattacc aggtgccgtt aggcgagttg
      301 cgcgccgagg tgaaaaggct ggtggatggg aacgaaaaat gggaccgcca ggtgtgtggc
      361 ctgcaggtca ccgacgcgaa agaaaggacg ctcgaggtgc gcgtcgtagt gagcagcaca
      421 aatgcgtcca aaaatttcga cctgcgctgc gaggtgcgcg aagggctgat cgaattcctg
      481 cgcaccaagt atccggaaag tctgccgcgg acgcggctga cattgcaacg ccacgaggag
      541 accggccagc tgccggccca atacggcatg gcggttgaag gaaaagagca cgaacgcggg
      601 tcgaccgccc cggggttgga gccgaaggat tccgatggag ccgctcggcg ttgagtagac
      661 ggttcgctct tggaagcgcc acccagagga aggcgtctgc atgcgtagat cgaacatctt
      721 gcacttccgg cgcaaaaatc gtagaaacgg aggatgttcc aaaaggatgc gatcgtgcgg
      781 ctcctgcgcg acaacgatcc ggccaccgtc actttgctga aggcgcaact catcgattcc
      841 ggaatggaag gcgccgcgga tttgcgcgat ttgctcgcgc tggatgacgc agcggtgacg
      901 gcgcacgtgc gcgaggtgat cgagaaaatc gagaccgatg aagcgcagga tgaaatgctg
      961 ctctgcgcac atttgtttcc ggaacacggc gacatcgagg cggcgtggtg gctcttgacg
     1021 ctttgttttg aaccggacgc gccgattgcg aaattacggc gcaggctcga cgcgtgggga
     1081 cggcggctgc gcatgctggt cgcgaaagcc gggtcggcgc gcgagcggct ccaggcgctc
     1141 acgacttttc tggcggagga tctcgggttc cgcgggaacg cggaagagta ttacgatccg
     1201 aagaattcgc tcttgagcga cgtgatcgag acgcggctcg gaattccgat ctcgcttgcc
     1261 atgctctatg taatcgtcgg ccgtcgcgcc aatatgcaga tcgacgggat caatttgccc
     1321 ggccatttca tcgcgcgtta cgacagaata ctgttcgatc cgtttcacca ggggcgaatc
     1381 ctctcgcggc cggattgtga ggcgattctc gcccggcaac ggctaaaaac ggagtcgagt
     1441 tatttcgccc cggcctcgcc gcgacttgtc ctcatgcgga tgctggccaa tctgctcttc
     1501 attttcgaac gcgcgggcga aaagcagaag caccaacttg tgatgaactg gttgaaggca
     1561 ttggatcgcg actgactgtg tccggggtcg atgaccccgg tggctcactg tagccgcggg
     1621 gcgagaagcg ttttcgcagg cgtccggggt caacgccccc ggctttggaa cagctcttcg
     1681 agttgaattt gccgtgcccg agcctggcgc gcgttgtttg ctttggcccg gtcgtggcgg
     1741 aagacttggc cgcgtttcag ggccggaatt ctcccgccca gttgctccag aacgtgaacc
     1801 gcggcgccgc gcagcgacgc ttcctgatcg aggcaagttt ccagatcgtg cccgagggag
     1861 tcggcgagga tttgcagcga cgcaggagat tgtaatatcc cgccggagac gattactctt
     1921 ttcgcgggac cgatcgcacc ctccagctgc tcaagaatct gggccagccg gtgatacacc
     1981 gcggcggtcg tggcgcggag gagatcgacc gcggtgctcg ccagggtgac gccgaggatg
     2041 gcgccgtcga gatgctcggg ccagctaggc gcgcgttcgc tcacccagaa gggcagaatc
     2101 gtcaggcgcg cggtcgccgc ggacggctga cgcagggcct tctccagcgc gcgctcgtcg
     2161 tctggcagcc gaagttcgcg ccggcaccag gcgcgcaggt tgccggcgtt gctgaccgcg
     2221 ccgccaagaa gggcgcgttt ttgatcgacg acaaaacgga agagaccgaa aggcagacgc
     2281 gcattcgccg caggtattgc gcggacggcg gcgctggtcc cgatgttgat cgccacaatt
     2341 cccgggcgac tggcgccgga gccaaggttg ctggcggcgc cgtcgccgat cgcggggaag
     2401 acggtcacgc catcgaggcg caccgcgtcg cggagcgggc cgagccgcga agtggttaag
     2461 ccagaaattt cgagaagatc cttgtcccag tcgcgtcgcg caaaattgta aaggcccgtg
     2521 ccgctcgcca tcgagtgact gcagccgcgg gctccgaaag ctttttcgaa aatccactcc
     2581 gcgggtgaga cccagcgcgt cacgcgacga aataatcgtg gttgcgtgcg ccgcagccaa
     2641 aggagcttgg ccggccaaaa agaggcgcgc agcatgcaac cggtgcgttg ctggatggcg
     2701 cgctcctcga gtctttcgcg cagacgggcg gcatcctcgg cgcagcgcgc atcggcccag
     2761 gtataaatcg gtgtgagcgg ttcgcccgca cgatcgagcc cgagcaggct gtgccagaaa
     2821 ccggaaccgc cgatagctcg aaccgggctt ttcgtcaggc cgcgcgtctg ggaaaggcac
     2881 ttccttgtgg cttgccacaa gacggtcggg tcgagttccg cgccgtgctc ggcggaatgg
     2941 cggacccgat aaacctggct ggcggcggaa cccggaacgc gctgcgccct ttcgtcgaaa
     3001 agcgctgtcc ggacggagga ggtgccgatg tcgatggcca ggacggccgg tttgctagag
     3061 cgaggcatga gccaattaaa tcgtgttaag cttcaagttt taagctttaa gtggggaaac
     3121 gcggccccga agaccttcac ttaaaactta agccttaaag cctaaagctt ggtttcgatg
     3181 tacaaaaaaa tccttgtcgc gcttgataac agcagcaccg accaggcgat gtttcggcac
     3241 atcgccgagt tggccaaggt gcatgcggcg gagctgctgc tcgtccatgt ggccgacggc
     3301 tgggcggccc gaaactacaa tcgcttccag ctcgccgatt ccgaggagat gaaggaggac
     3361 cgcgcctacc tggagaaaga ggcggccgat ttgcgcgccc aggggctcaa ggtccagacg
     3421 cagcttgcgc tcggcgaccc ggccaccgaa attctcaagg cgtcgaccga ggagaaatgc
     3481 gacctgattg cgatgacgac gcacggtcac cgctttttca aggacttgct ttacggcagt
     3541 acgatcaccc aggtgcgcca tcgcgcgagg atgccggtgt tgctggtgaa cgttgcgaaa
     3601 tagcggcggc gcgacgtggt ttcctccgca tccgagagaa tgaagacgct tttcctgacc
     3661 aatgaatatc cgccgcacat ctacggcggc gccggcgtgc atgtcgagta cctctcgcgg
     3721 gagctggcga agttgatgga ggtggaggtg cgttgtttcg gcgatcagca atcgttaggc
     3781 acaaatccgg aagtgcgcgg tttcgagctc gcgacgagct cctacacctg cccgccgcga
     3841 ttgcagtcgg tcttcggcgc ggtgcaacgc tgtctcgatt tcaacaccct caacatcgcc
     3901 gccgacgtcg tccactgcca tacttggtac agccattttg gcgggatcct ggcgaaggtg
     3961 aattacggca ttccgctcgt cattacggtc cactcgctcg aaccgttgcg gccctggaag
     4021 cgcgagcaac tcggcggcgg ttatgacttc actgtctggc tggaaaggac cgcgctcgag
     4081 atggcggacg cggtcatcgc ggtctcgaac gggacgaagg ccgatgtggc acggctgttc
     4141 cagatcaaac cggaacgctt acacgtgatc tacaacggga tcgatctcga cgaatatcgg
     4201 ccgaccgatg cgaccgatgc gctggtgcgc catggggttg atccggcgca gccttatctg
     4261 ctttttgtgg ggcgaatcac gcgacaaaaa ggaatcattc atctcgtgca cgcgattcaa
     4321 ttcatggatc cggacttcca gatcgtcctc tgcgcgggcg cgccggacac cccggaaatc
     4381 gcggcggaaa tgaaagcggt cgtgggcgag gcgcaggcga aaaggaacgg catcatctgg
     4441 attgacgaaa tggtcgacag ggaaacggtg caccagctct acgcgcaggc cgcggttttc
     4501 tgttgcccgt cgatctacga gccgtttggg atcatcaatc tcgaggcgat ggcctgcgag
     4561 accgcggtcg tcgcgagcgc ggtcggcgga atcaaagagg tggtggtcga cgacgagacg
     4621 ggatttctcg tgcccgtcga gcagatgctg gaaagcccgt tcgaagcgat cgatcccgag
     4681 agatttgagc gcgatctcgc cgcgcgggtc aaccagttga tggccgatcc ggtgttgcag
     4741 agaaagttcg gacgcgccgg ccggcggcgc gcggaggaaa aatttggctg ggccgcgatc
     4801 gcccgggaaa caaagcacct ctacgagtcg ctcctcaaga agtagcggtg agcgacgcgg
     4861 gcgctgtgct cggggcggac tgtcgttatg cgcggcacgg tgtatcgtgt tacgatgttt
     4921 gtcgatcgga tcaaggtggt tgcgaagggg ggcgacggag ggcgcggctg cgtcagtttc
     4981 cggcgcgaaa aattcgtccc gcgcggtggt ccggacgggg gtgacggggg caaggggggc
     5041 gacgtcattc tccgcgccga cgagcacacg gataatctcg ccagtctttt ttacgagccg
     5101 ttgatcaagg cgaagaatgg ggcgcacggg atgggcaaac aaatgcatgg tcgcggcgcg
     5161 ctggcgaagg tcgtgaaggt gccggtcggc accgtcgtgc accggatcga ttcgttaggc
     5221 gaggcggccg cctttacgga tgccgcgggg gcggcggaag tgctcgcagt gcccggagcg
     5281 accctcgagc aaatcgcgga tctggccttg cctaacgaag agttcgttct ctgtcacggg
     5341 ggcaagggtg ggaaagggaa tgtccacttc aagagcgcgc gcaatcgggc gccgcgccaa
     5401 tactccgacg gggaagaggg cgaggagggg acttttgttt tcgagttgcg cacgatcgcc
     5461 gatgctggtc tcgtcggtta tccaaacgcc gggaaatcaa cccttctgcg acgcatctcg
     5521 gcggcgcatc cgaaagtggc gtcgtatcct tttaccacct tgcatccgat gatcggggtg
     5581 gtggagttcg ccgactaccg gcgcgcaacg ctcgcggaca tcccgggatt gatcgaaggg
     5641 gcacaccgca atctcggtct cgggcacgct tttctccggc atatcacgcg gtgccggttg
     5701 ctcctcttcg tgctcgatat ggccgggagt gaagggcgca atccgattga ggatctgcag
     5761 catctgcggc gtgaaatcga tctctacgac ccgcggcttt cgctgcggcc ctggcgggta
     5821 gttgcgaaca agatggattt gccggaggca gcagagaatc tcgccgcttt ccgcgagcgt
     5881 tttccagagc gggaggtcat cccggtctca gcgaaggaaa atgagggcat cgaaaacttg
     5941 aaacgcgctt tggatcgctg gctcaatgag ccggaattgg agttgccagg gccgcgtgcc
     6001 gaaggataca cgattagagt tagacaagcg gaggcaatgc ggtaagagtt cacgactttt
     6061 tggcgctacc atcaggaggt tccccatcta ttatgtctaa actggtcgtc gcaagttact
     6121 gcaccacatt cctgaaatcg gagatgctcc acatctaccg gcaggtcacg gccctgcgcg
     6181 atgtgcagac cttcgtgatg acgaaaaagg tgcaaaacaa cgatcgcttt ccgttcgatg
     6241 acatcgagtt gatcccggcg ccgcgtccga atccgttccg gcatggctgg ttgaagtttg
     6301 tcgagcggca gccgccgatc gtttatcggg gcgaatacca actgctcgcc aacttactcg
     6361 aacaccgggg cgcggacctg atgcatatct atttcggcca cacggcggtg catctgttgc
     6421 cgtttatcga gtgttggaac aaaccctgca tcgtctcgtt tcacggcgcg gatgtgatgg
     6481 tgaaagcgga aagcccggat tacgccgcga agttgcgccg ggttttccag tccgtcccgc
     6541 tcgtcctggc ccgttcgcgc tcgctcgagc aacgcctcat cgtgctcggt tgcccgcccg
     6601 aaaagattcg aatcaatcgg acggggattc cgcttgagca atttccgctg gtccgccgac
     6661 aggctcccga gggaggacgg tggaggtttc tgcaagcgtg ccgcttgatt ccgaaaaaag
     6721 gcctaacgac ttgtttgcgc gcctttgccg ttttccgacg agagaatccg ggggcagagc
     6781 tgctgatcgc cggaaagggg cctttgcaac ccgtcctcga agcgctcgcc gcgcaattgg
     6841 acatcagtga tcacgtgcac ttcaccggtt ttctttcgca gccggatctg ctcgcgcttt
     6901 accacagttg ccacgtcttt ttgcacccga gcgaaatgcc ggcggacgaa aaccaggaag
     6961 gaattcccaa ctctatcctc gaggcgatgg cgaccggcat gccggtcctc gcgacgcggc
     7021 atggcggaat tcccgaagcg gtggaagagg gccgctgtgg agcgttagtc gaggagcgcg
     7081 actatctggc gctggccgcc gagatgaaga agatcacgcg ctcgccgcat tccttcggtg
     7141 agatcggcgt cttggcgagc gaatccgtcg ccgcccattt cgagcagcgc gtacacatcg
     7201 cgcaactgga g
//
LOCUS       DATMNS010000042        11494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3248762, whole genome shotgun sequence.
ACCESSION   DATMNS010000042 DATMNS010000000
VERSION     DATMNS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 11494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11494
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3248762"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1099)
                     /locus_tag="VNW28_01605"
     CDS             complement(<1..1099)
                     /locus_tag="VNW28_01605"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXA08645.1"
                     /translation="MHSLFSSSSGVPHRPKSCDICRLTILLAGGAVASLALSQTASAQ
                     VGTVPRIEVTGQAVPSPTPHDDWKAKLDHIMSEVSGTQITVTKKATVIKLDQQPTVVG
                     NNLQELFTKAPGLLITEQQTPGQFNFSYRGLGNPQESEFVTVLMDGVPLASDWIGFPT
                     LYFLPLSQSVSEIQEIRGGSSLLYGPEPAPAVNFVTKHPAPGSPWNFYTEQIGGSNGL
                     YQTYNVFQEAYGPVEFRLDGGYVRSDGQRDNSQYDEWQSNLYLGYRPNEEQLLALDFH
                     ASRFSGGDPGRLTFPQFRQNEDLSTAPFDHDWVDRYSVVLRDEWAFANGWLLQAKGWY
                     THQDIDSRNESFFGPAPHPTTLQLEEFNNGGA"
     gene            1353..2615
                     /locus_tag="VNW28_01610"
     CDS             1353..2615
                     /locus_tag="VNW28_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXA08646.1"
                     /translation="MNNPRSVNVCGAGIVGLCTAHYLAREGLAVTVIERNAEGTDSCA
                     HGSAGFVSPSHVAPISAPGMVWQGLKWMMSSRSPFYIKPRFDPGLVRWGWLFARNCTV
                     EHTRRAAPVLRDLCLAARELFLELAETTGNAFELRTEGMLNLCKTPEALEHEMHGLGR
                     LANELGVEARLLDARQTAELEPGARLDVAGAIYFPSDAHLTPRKLIPTLVALLQQEGV
                     SFHWKTSVHGWNCEAGRITSVSTSKGEFVADEYVMATGSWAPATVRGLGLRLPMQPGK
                     GYSLTIEQPRFKFTKSLILAERRVAVTPMGDQLRFGGTMEISGHTNNVRPERVAQIIA
                     AAQDYFPDFRAKDFAGIKPWFGYRPVSPDGMPYIGRFARPANLTAACGHAMLGLTLAP
                     ITGLLVAETLTGRRPSIDMRLLSPDRFA"
     gene            3151..3552
                     /locus_tag="VNW28_01615"
     CDS             3151..3552
                     /locus_tag="VNW28_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXA08647.1"
                     /translation="MLRILNNRRKGFTLVEIMIVVAIIALLAAIAVPGFLRARKRAQA
                     SRILNDLRLIDSAVDQYAIENSVPTGTTVPITAWTAYMKANSLLYNTGQDMFGDPYGA
                     QTVDSLPKVPANAWNTLSDVAGTTFFSPYGP"
     gene            complement(3617..4339)
                     /locus_tag="VNW28_01620"
     CDS             complement(3617..4339)
                     /locus_tag="VNW28_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXA08648.1"
                     /translation="MQRARLELSPGLWLDARRAVWMEKQRTLLVADLHLGYAWAHREQ
                     GNLLPVGTAEDTLDRLQAIATEYAPREIIVLGDVIHAAVKEDTVLGELRRLHALGAPV
                     RLIAGNHDSQLAPMLHRAGIDAVLLREITLAENLLLHGDLGNETMAEARCLSIAERGG
                     RVILGHEHPAITLSDGVATRVHCPCFLVGDDVLALPAFSPWSAGTNVRHGVFLSPYLR
                     RAHLTQTIAIVANRLLPMPASR"
     gene            complement(4514..7138)
                     /locus_tag="VNW28_01625"
     CDS             complement(4514..7138)
                     /locus_tag="VNW28_01625"
                     /inference="COORDINATES: protein motif:HMM:NF020083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXA08649.1"
                     /translation="MSSFAGNSRVFRWFRARYGRPTPAQRDAWPRLARGENVLIASPT
                     GTGKTFAAFLSVLDALAQEHACRKLSNTIHCVYVSPLRALGYDLEKNLAEPLRGLFGR
                     KPPIRVELRTGDTTASQRARQFSKPPHILLTTPESLCVLLSQEKWLPQFCHVRWLIID
                     EIHALADNKRGAHLALSVERLDDLQLKARSAALQRIGLSATVAPLVEVAQFLVGTHGR
                     CAIIDVATAKKIRLRVHTPLRKNPYPEAGFTGQRLVRELGNLIRQHRTTLVFSNTRSG
                     AEGMTYWLREKFPELAEFIECHHASLERDVRREVEDRLKRGELRAVICSTSLELGIDI
                     GSIELVVMLSTPKGVSKALQRAGRAGHNIHAVSRGILMATNVSDLVESCATVLLARAR
                     HLDEVRVPRAPLDVLAQHLVSMGCTAHWTRTDALALVRRAFPFRDLDAEELDNVLDYL
                     AGGGKSLRQQYSEVFGKIALDDDGFRTRPGRVQREFLQNIGVIPSVGQVRVRLKRQSL
                     GSVEEFFIRMMKIGDVFVLGGRPVRLEKVNQMEASVTRADGALPTIPRWNASKFPLSN
                     RVAQEIVAFRRELRARIEGSLPNDAVEDLAPWVARRLDCGKANGRIVASIHLAQHQLS
                     EIPTADCLLVEEFHETNAAPVIKKNPHRNPHAIHYFFHSLIGRAANDALARVVTLRLS
                     QLRGGNAVATPHDYGFVLTVAPTQRVAEQDLRTLLRVEGFETELERSLAQSEMLKYHF
                     RNAAQTGLMVYRNFFDQKKSVRKLAWSSEVIFNVLQQYEPDHILLREARRDTLRIFLD
                     AESALAFLHAQTAKPVRLRPVAQVPPLSFAMYATKMKEALLVEDPWEVQERLFHHWWN
                     AIEESSISATETRMGSPD"
     gene            7280..8068
                     /locus_tag="VNW28_01630"
     CDS             7280..8068
                     /locus_tag="VNW28_01630"
                     /inference="COORDINATES: protein motif:HMM:NF015921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08650.1"
                     /translation="MHHHRDAVREGVLSKVPQITFAFWLIKICATTLGETGGDALSMS
                     LELGYAVSTAIFFTILLVAVIAEVSAHTFHRFRYWTVIVATTTVGTTMSDYLTRTAGL
                     GYFWSSVLLFSIVMAVLTVWRLSLGTVSVNKITNRQAEIFYWVTILASNTLGTALGDC
                     LADTTPLGYEGGALVFAGALALVLVAYLWTNISHTTLFWAAFILTRPLGATLGDIVTK
                     PVAHGGLNLSRISSSLAIAAFIFVAILFTSRGAGKHHGAKEEST"
     gene            complement(8246..9289)
                     /locus_tag="VNW28_01635"
     CDS             complement(8246..9289)
                     /locus_tag="VNW28_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08651.1"
                     /translation="MLWKFFLGTLAGALALQNAVASEGQYQFIREIPIGGEGGWDILT
                     VDSTAQRLYLSHATKVVVIDLEKNAVAGEIADTPGVHGFVAVPEFGRGFSTNGKENKS
                     SMVDLKTLKTIAKIDTGENPDALVYEPDRREVYVFNHTGHSATVIDAKTAKVVGTIAL
                     GGSPEFAAVDPGANRVYCNIEDKSEVVAIDTAKHEVVARWPVAPGEEPSGIAFDAAHH
                     RLFSGCHNKMMTMLDTESGKVVATVPIGAGVDGCAFADATQLAFASCGDGTTTIAKEE
                     SPAKLTVVQTLKTEPRARTITIDPGTHRIYLPSARFNPPAPASPGTTPARPTVIPGTF
                     KLLVYGPAATPQP"
     gene            complement(9363..9563)
                     /locus_tag="VNW28_01640"
     CDS             complement(9363..9563)
                     /locus_tag="VNW28_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08652.1"
                     /translation="MKIRSAELENECGALVCSFELAKSGSRDITEVLVNAKTGKIISV
                     STETRVDPAKETAADHAALRQE"
     gene            9863..10993
                     /locus_tag="VNW28_01645"
     CDS             9863..10993
                     /locus_tag="VNW28_01645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA08653.1"
                     /translation="MNSAPSFAVVVPFFNEERNVGAICKELRGVLDGELATSEVILID
                     DGSSDRTGAKLDEIALGWPACRVFHLGENQGQSAALLFGFGKSTAPVIVTMDGDGQND
                     PRDIVRLLPLLDDADMVVGARVSRQDPWMRRRISRIANSIRSRALSDGVSDAGCALKV
                     FRRAVVGAFIPIRTLYSFMPALAVGAGFRVIETPVNHRARRDGTSKYTVHSFFLLPIL
                     DLIGLRWFRSRRCQVPSLQVRDAHAATMNLGDELYSRALRRWTRMAALGVAICLIGSG
                     IMLLPREGRTSQRIGLYRAERIALHDVPKGRVGSAELSMQEDRLVWTIDVQPPAAADF
                     REIEIDARNGHVIAARTESAEEEALEVAVEEHRPLPKNVIPR"
     gene            complement(11205..>11494)
                     /locus_tag="VNW28_01650"
     CDS             complement(11205..>11494)
                     /locus_tag="VNW28_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08654.1"
                     /translation="SVPSAVLSDDPEWVQRHALLDSSRRLVPAAPNELRRAYGATLGC
                     FRSGDENYARRLRQCSAAQLIDRVHETVRRIRRQPAIVRPKDFESVRPYAL"
BASE COUNT         2113 a         3419 c         3669 g         2293 t
ORIGIN      
        1 cggctccgcc gttgttaaac tcctctagct gcaaggtggt agggtgcggc gccggcccga
       61 aaaagctttc gttgcgagag tcaatatcct ggtgcgtgta ccagcccttg gcttgcagga
      121 gccagccatt cgcaaaagcc cattcgtcgc gcagcacgac cgagtaacga tcgacccagt
      181 cgtgatcgaa aggcgcggta gaaaggtctt cattctgccg aaactgcggg aaagtgaggc
      241 ggccggggtc gcctccgctg aagcggctgg cgtgaaagtc cagagcgagc agttgctcct
      301 cgttcgggcg atagccaaga tagagattcg attgccattc gtcgtactgg ctgttgtccc
      361 gttgtccatc ggaccggacg tatcccccat cgaggcggaa ctcgaccggc ccataggctt
      421 cctgaaagac gttgtaggtc tggtaaaggc cattggatcc accgatctgt tcggtgtaaa
      481 aattccaggg tgaaccggga gcgggatgtt tggtcacaaa gttgacggcc ggggcgggct
      541 cgggtccgta aaggaggctg ctgccgccgc gaatctcctg gatttcgctg acactttgag
      601 aaagcggcag gaagtagagc gttgggaatc cgatccaatc gctggccagc gggacaccgt
      661 ccatcaggac tgtgacaaat tctgattcct gcggattgcc cagcccgcga tagctaaaat
      721 tgaactgacc cggcgtctgc tgttcggtga tcagcaagcc tggggctttg gtgaaaagct
      781 cctgcagatt gtttcccacc accgtcggtt gctgatcgag cttaatcacc gtcgccttct
      841 tggtcacggt gatttgcgtc ccagaaacct cgctcatgat gtgatcgagc ttcgctttcc
      901 aatcgtcgtg cggtgtcggg ctgggcacgg cctgcccagt gacttcgatt cgtggcaccg
      961 tgccaacctg cgcggaagca gtctgactga gcgcgaggga agcgacggct ccgcccgcaa
     1021 ggagaatcgt taggcggcaa atgtcgcaag attttggacg gtgaggcacg ccggacgagg
     1081 aagagaaaag tgaatgcatt tgggcgggat tacatttttg tcatcttgga ggcaagtttt
     1141 ttttcgctgc cggaaagttt ttttgtccgg ctgttttcgg atgggcgatt tcgatgcaag
     1201 atccggccgc ttccattttg tgtccgagtg gactttttct cgaatccagg agacgacgca
     1261 caacctccca gagatcgcag ccggttcagg ctctcgttag gcaagctggg tgtgcgcttg
     1321 tgtcgggcgc cgtttcggac ataagaaggg ccgtgaacaa tccgcggagt gtgaacgttt
     1381 gcggtgccgg catcgtcgga ctgtgcaccg cgcattacct ggcgcgggag ggtctcgcgg
     1441 tgacggtcat cgagcgcaac gcggagggca ccgattcgtg cgcgcacggg agcgccggct
     1501 tcgtctcgcc gagtcacgtc gcgccgatct ccgcgccggg gatggtctgg cagggcctga
     1561 aatggatgat gagttcgcgg agcccgtttt acatcaagcc gcgcttcgat cccgggctcg
     1621 tgcggtgggg ctggctcttt gcgcggaact gcaccgtgga gcacacgcgc cgggccgcac
     1681 ccgtgctgcg cgatctctgc ctggccgcgc gagaattgtt cctggaactg gcggagacga
     1741 ccggcaacgc cttcgaactc cgcaccgagg gcatgctcaa tctctgcaaa acacccgagg
     1801 ccctcgagca cgagatgcac gggctggggc gcctcgcgaa tgagttgggc gtggaagcgc
     1861 gcttgctcga cgcccggcaa accgccgagc tggagccggg tgcgcgcctg gacgtcgccg
     1921 gcgccatcta ttttccaagc gacgcgcacc tcacgccgcg aaagctcatc ccgacgttgg
     1981 tcgcgctgtt gcaacaagag ggagtcagtt tccactggaa gacgagcgtc cacggctgga
     2041 actgcgaggc cgggcgcatc acgagtgtct cgacgagcaa gggagaattc gtcgcggacg
     2101 aatatgtgat ggcgaccggt tcgtgggcgc cggcgacggt gcgcggtctt ggcctgcgcc
     2161 tcccgatgca gccgggcaag ggctatagcc taacgatcga gcagccgcgg ttcaagttca
     2221 ccaagtcgct catcctggct gagcgccgcg tggccgtgac tccgatgggc gaccaattgc
     2281 gcttcggtgg cacgatggag attagcggtc acacgaataa cgtgcggccc gagcgggtcg
     2341 cgcaaatcat cgcggcggcg caggactatt ttccagattt tcgcgcgaag gactttgcgg
     2401 ggatcaagcc atggttcggt taccggccgg tctcgcccga tgggatgccc tacattgggc
     2461 gcttcgcgcg gccggcaaat ctgacggcgg catgcggcca cgccatgctg ggtctgacgc
     2521 tggcgccgat caccggcctg ctcgtggctg agacactgac cggccggagg ccgagcatcg
     2581 acatgaggct gctaagtccg gatcgctttg cctaagacgc gaggtcgatc ctcagacgac
     2641 cgattgacga ttcacgtctt ctcgatttct cttgcctttg tcccgcgggc ccaacgggtc
     2701 gcgatcatta actgacattc tatactcggg gcctgaagga atgtttgcta tcactggtga
     2761 agtagaaagt ggtcgcttcg aggaaaagcg cggactcctt gcacgatgta tgagtagttt
     2821 tcgattcgct aaagctaatc tgacccatgc tcaactccaa gcggctgtca tgaacttgcg
     2881 ggtttgcgcg gccaacgaaa tccacgtcct gtagccgtcg ccctgtgagc gacgtgggtg
     2941 cgatgggtcg gttcgccttg ctgcgcacat accccacgct tcgcacagcg aagcggctac
     3001 agcaggcagg ctggcccgtg ctcctgacat ttggattagc aacgaatgac aaagataagc
     3061 gtgttatgaa gatcactcgg gttggtatca gcgatgctcg taaagagggc aatccgcgtt
     3121 ggaacggcgg atgcaatcaa aacaatcaat atgttacgaa tactcaacaa ccgccgcaag
     3181 ggcttcaccc ttgtggaaat catgatcgtc gttgcgatca tcgccttgtt ggccgccatc
     3241 gcggtcccgg gatttctccg ggcccgcaag cgcgcgcagg cgagtcgcat cctgaacgat
     3301 ctgcgcctga tcgactccgc ggtcgaccaa tacgccatcg aaaacagcgt gccaaccggg
     3361 acgacggtcc ccatcactgc ctggaccgcc tacatgaagg cgaactctct gctctacaac
     3421 acgggccagg atatgttcgg cgacccgtac ggtgctcaga ccgtggatag cctgccgaag
     3481 gttccggcca acgcctggaa caccttgtcc gacgtcgcgg gaaccacctt tttctcgcct
     3541 tacgggcctt aacaggaagc ggtcgagtca ttcgcctgcc atccccgact ggccacaatg
     3601 tgagttaagc taacgctcag cgcgaggcgg gcatcggcag gagccggttc gctacgatcg
     3661 cgatcgtctg ggtgagatgc gcgcggcgca ggtagggcga aagaaagacg ccgtggcgca
     3721 cgttcgtgcc ggctgaccag ggcgagaagg ccgggagcgc gagcacgtcg tcgcccacga
     3781 gaaaacaggg gcagtggacg cgagtcgcga cgccgtcgga aagagtgatc gcgggatgtt
     3841 cgtgacccag gatgacgcgt ccgccgcgtt cggcgatcga aaggcaacgg gcctcggcca
     3901 tcgtttcgtt gccgaggtcg ccgtggagaa ggagattttc ggccagggta atctcgcgca
     3961 gcaagacggc gtcgataccg gcgcggtgca acatcggcgc gagctgactg tcgtggttgc
     4021 cggcgatcag gcggactggg gcgccgagcg cgtgcaaccg gcgtaactca ccgagcaccg
     4081 tgtcttcttt gaccgcggcg tgaatcacgt cgccgagcac gatgatttcg cgcggcgcat
     4141 attccgtcgc gatggcctgg agccggtcga gcgtgtcttc ggcggtgcca accggaagga
     4201 gatttccctg ttcgcggtgc gcccaggcgt agccgagatg caaatccgcg acgagcagcg
     4261 tccgctgttt ctccatccag accgcgcggc gcgcatcgag ccagaggccg ggtgaaagtt
     4321 cgagacgggc gcgctgcatc gatgttctca gtttagacct tgtcgtacac atcgcgactg
     4381 gagggacggg cgccgtcccg tccgagtcta tgaagcccct aagagaaaca cgcacgaaac
     4441 ccgccgcggc gggcaggatc tggtcgtccc tccacgctcc aggctttacc cactaacgac
     4501 gtgcaggaca ccctcaatcc gggctcccca ttcgtgtttc cgtcgcagaa atcgaagact
     4561 cctcgatcgc gttccaccaa tggtggaaga gccgttcctg cacttcccac ggatcctcca
     4621 cgaggagtgc ttccttcatc ttggtggcat acattgcgaa cgaaaggggc ggcacttgcg
     4681 ccactggccg cagtcgcacc ggtttcgcag tttgcgcgtg cagaaaggcg agcgcgcttt
     4741 cggcgtcgag gaagatgcgg agtgtgtcgc gccgcgcttc gcgcaggagg atgtgatccg
     4801 gctcgtattg ctgcaacacg ttgaagatga cctcgctgct ccaggccagt ttgcgcacgg
     4861 attttttctg gtcgaaaaaa tttcggtaaa ccatcaggcc ggtctgggcc gcgttgcgga
     4921 agtgatattt cagcatctcg gactgggcga gcgaacgctc gagttcggtt tcaaaaccct
     4981 cgacgcgcaa gagcgtccgc aggtcttgct ccgcgacgcg ctgcgtcggc gcgaccgtaa
     5041 ggacgaagcc gtagtcgtgc ggtgtcgcga ccgcgttgcc gccgcgcagt tggctgagcc
     5101 gcagcgtgac gacgcgggcg agggcgtcgt tcgcggcgcg gccaatgagc gagtggaaaa
     5161 aataatggat cgcgtgcggg ttgcggtgcg gatttttttt tatcacgggc gctgcattgg
     5221 tctcgtgaaa ttcttccacg agcaggcaat ccgcggtcgg aatttccgag agctggtgct
     5281 gcgcgagatg gatgctcgcg acaattcgtc cattcgcctt tccgcaatcc aaccgtcgcg
     5341 cgacccaagg cgcgagatcc tctaccgcgt cgttaggcag cgacccttcg atccgcgccc
     5401 gcaattcgcg gcgaaacgca acgatctcct gcgcgacgcg attcgagagc ggaaacttgc
     5461 tcgcgttcca gcgcgggatg gtgggcagtg cgccatcggc ccgcgtcacc gacgcctcca
     5521 tctggttcac cttctcgagg cgcaccgggc gtccgccgag cacgaacacg tcaccgatct
     5581 tcatcatgcg gatgaagaat tcctcgacgc tgccgagcga ctgtcgcttc aaccgcaccc
     5641 gcacctgccc gacgctcggg atgactccga tgttctgcaa aaattcccgc tgcacccggc
     5701 cgggccgggt gcgaaagcca tcgtcgtcga gtgcgatctt gccgaagact tcggagtatt
     5761 gttggcgcaa cgattttccg ccgcccgcga gatagtcgag aacgttatcg agttcctccg
     5821 cgtcgagatc gcggaaggga aaggcgcgcc gaaccaacgc gagtgcgtcc gtgcgtgtcc
     5881 agtgcgccgt gcagcccatg ctcacgagat gctgcgcgag cacatcaagc ggcgcgcgcg
     5941 ggacgcggac ttcgtcaagg tgccgggcgc gggcgaggag aacggtggcg cacgactcaa
     6001 cgagatcgct cacgttcgtc gccatgagaa tgccgcggct gacggcgtgg atattgtgcc
     6061 cggcgcggcc ggcacgctgc agcgccttgc tcacgccttt cggagtcgag agcataacaa
     6121 cgagttcgat cgagccgatg tcgatgccga gttcgagact ggtcgagcaa atcacggcgc
     6181 ggagctcgcc gcgtttcaaa cgatcctcga cctcgcgcct aacgtcgcgc tcgagcgagg
     6241 cgtggtggca ctcgatgaac tccgccaact cgggaaattt ctcgcgcagc cagtaggtca
     6301 tcccttccgc gccggagcgg gtgttggaaa agacgagtgt ggtgcggtgt tgccggatga
     6361 ggttaccgag ctcgcgcacc agccgctgac cggtgaagcc ggcctccggg tatgggttct
     6421 tgcgcaacgg cgtatgaacg cgcagacgga ttttcttggc ggtggccacg tcgatgatgg
     6481 cgcagcgccc atgcgtgccg acaagaaact gcgcgacttc gacgagcggc gcgacagtcg
     6541 ccgaaaggcc aatgcgctgg agcgcggcgg agcgcgcctt gagctgcaaa tcatcgagcc
     6601 gttcgacgct gagcgcgaga tgcgccccgc gtttgttgtc ggcgagggcg tggatttcgt
     6661 cgatgatcag ccagcgaacg tggcagaact gcgggagcca tttttcctgg ctgagcagga
     6721 cgcaaagact ttccggcgtg gtgaggagaa tgtgcggcgg tttggaaaat tgccgcgcgc
     6781 gctggctggc ggtggtgtcg ccggtgcgca gctcgacgcg gatcggcggc ttccggccga
     6841 agagtccgcg caaaggttcg gcgagatttt tttcgagatc gtaaccgagt gcgcgaagcg
     6901 gggagacgta aacgcagtgg atcgtgttgc tcagttttct gcaggcgtgt tcctgcgcga
     6961 gcgcgtcgag cacggagaga aaagcggcga acgttttgcc cgtgccggta ggcgaggcga
     7021 tgagcacgtt ttctcctcgc gccaggcgtg gccaggcgtc gcgctgcgcc ggcgtcgggc
     7081 gcccgtagcg cgcgcgaaac cagcggaaga cgcgcgagtt gccggcgaaa gaagacatgc
     7141 gttgggaaaa tccgcgcggg atacgcgctg gcaattgcaa tccggcttac gaaccaaccg
     7201 gagacgcgtc acgctgcgat tgtgccagga cgaccgcatc cttgaaatga catcgcacca
     7261 tcattagcta agaaaaaaca tgcaccatca ccgagatgcc gttcgcgagg gcgtgttgag
     7321 caaggtcccg cagatcacct ttgcgttctg gctgatcaag atttgcgcga cgaccctggg
     7381 cgagaccggc ggcgacgcgc tctccatgtc gctcgagctc ggttacgcgg tcagcaccgc
     7441 aatttttttc acgatcctcc tcgtcgcggt catcgccgaa gtcagcgcgc acacttttca
     7501 ccggttccgc tattggaccg tgatcgtggc gaccaccacg gtcggcacga ccatgtcgga
     7561 ttatctcacc cgcaccgccg ggctcggtta cttttggtcg tccgtccttc tcttctcgat
     7621 cgtgatggca gtcctcacgg tctggcgcct ctcgttaggc acggtctcgg tgaacaaaat
     7681 cacgaaccgc caggcggaga tcttttattg ggtgacgatt cttgcctcca atacactcgg
     7741 caccgcgctc ggtgattgcc tcgcggatac caccccgctc ggctacgaag gcggcgcgct
     7801 cgttttcgcg ggcgcgctgg cgctggtcct cgtcgcttat ctctggacca atatctcgca
     7861 cacgacgctt ttctgggcgg ctttcattct cacccggccc ctcggcgcga cgctcgggga
     7921 tatcgtgacc aagccggtcg cgcatggcgg attgaatctc agccggatca gctcgtcgct
     7981 cgccatcgcg gctttcattt tcgtggcgat tctcttcacg tctcgcgggg cggggaaaca
     8041 tcacggtgcg aaggaggagt ctacctaggg actgtcatgc agtttgccac agaggagctg
     8101 tagccgcttc gctgtgcaaa gcgtaggtat gtgggtggcg aagggggttg gcgcgtcgta
     8161 cccacgtcgc ccacagggcg acggctacag gacgcttgca ggccgcgcaa acccgcaagt
     8221 tcatgacagc ctccagacgc gcggcttatg gctgcggcgt ggcggccgga ccgtagacca
     8281 gaagcttgaa cgtacccgga atgaccgtgg gtcgtgccgg agtcgtgccg ggcgacgccg
     8341 gagcgggcgg gttaaatcgc gcggaaggca ggtagattcg atgcgttccc ggatcgatcg
     8401 tgatggtgcg agcgcggggc tccgtcttga gcgtctgcac gacggtgagt tttgccggag
     8461 actcttcttt cgcgattgtc gtcgtgccat caccacagga agcgaaggcg agttgagtcg
     8521 cgtcggcgaa ggcacagccg tcaacacctg cgccgatggg cacggtcgcg acgaccttgc
     8581 ccgattcggt atcgagcatc gtcatcatct tgttgtggca acccgagaaa agccgatgat
     8641 gcgccgcgtc gaaggcgatg ccactcggct cctcgccggg agcgacgggc cagcgtgcga
     8701 ccacttcgtg cttcgcggtg tcgatcgcga cgacctcgct cttgtcctcg atattgcagt
     8761 aaacacgatt cgccccgggg tcgactgcgg cgaattcggg actgccgccg agcgcaatcg
     8821 tgccgacgac tttggcggtt ttcgcgtcga tcaccgtcgc ggaatgaccg gtgtgattga
     8881 aaacgtaaac ttcgcggcgg tccggttcgt agaccagcgc gtcggggttt tcgccggtgt
     8941 ctattttcgc gatcgtctta agcgttttca gatcaaccat actcgatttg ttttctttgc
     9001 cgttggtcga gaaaccgcgc ccgaactccg ggaccgccac gaagccatgc acgccgggcg
     9061 tgtccgcaat ctccccggcg accgcgtttt tttccaaatc aattacgacc accttggtcg
     9121 cgtgcgaaag ataaagccgc tgcgccgtcg aatcgacggt cagaatatcc cacccgcctt
     9181 cgccgccgat cgggatttcc cggataaatt ggtattggcc ctcgctcgcg acggcgttct
     9241 gcaaggcgag cgcgcctgct aacgttccaa gaaagaattt ccaaagcatc aataatccta
     9301 acacattccc gtcgtcgctt ccggcggatt tggtgaggcg ataacgcccc ggcgacaacg
     9361 gctcactcct gtctaagcgc ggcatgatcg gcggccgttt ctttcgccgg atccacgcga
     9421 gtttccgtgc tgaccgagat gatcttgccg gtcttcgcat tcaccaggac ctccgtgata
     9481 tcccggctgc ccgatttcgc gagctcgaaa gagcagacga gtgcgccaca ttcgttctcc
     9541 agttccgcgc ttcgaatttt cacttcggcg ccttcgttag ggcgattttc tccgcctgtc
     9601 gcctggctga tttttgcctg tcaaatgagg tccgttttgc tcagagcggc aaaccccgaa
     9661 gtggtgacga acgttgtcgt taggccgggg gcgcaaacca atcggtattt tttcttcatg
     9721 cagactccgg cgacgcggcg gccagactcg ttcggtttcc ggcgggccgc ggatcggcgg
     9781 agtaaagggg ggcgccggtt ttccattgac ttggagggca gcgtggcgca aatgctctcc
     9841 gagttcacgc gaactccttg gcatgaatag cgcaccctcg tttgcggtgg tggtgccgtt
     9901 cttcaatgaa gagcgcaacg tcggagcaat ctgcaaagaa ctccgtggtg tgctggatgg
     9961 cgaactggcg acgagcgaag tcatcctgat cgatgatgga agcagcgacc ggaccggcgc
    10021 gaagctcgac gagatcgccc tgggttggcc ggcctgccgc gtttttcatc ttggcgaaaa
    10081 tcagggtcag tcggccgcgc tgcttttcgg ctttggcaag tcgaccgctc ccgtcattgt
    10141 caccatggat ggcgacgggc agaatgaccc acgcgatatc gtcaggctgc tgcccttgct
    10201 ggacgacgcc gacatggtgg tgggagcgcg agtcagccgg caggatccgt ggatgcgtcg
    10261 ccgaatttca cggatcgcga attcgattcg ttcccgagca ctgagcgatg gtgtttccga
    10321 tgcgggctgc gcgctgaagg tttttcgtcg ggcggtcgtg ggcgccttca tccccattcg
    10381 caccctttac tccttcatgc cggcgctggc cgtgggcgct ggtttccggg tcatcgagac
    10441 gccggtcaat catcgtgcgc gccgggacgg aacttcgaaa tataccgtcc acagtttttt
    10501 tcttttgccg atccttgatt tgatcggtct gcgctggttt cgttcacgcc gctgtcaggt
    10561 tccttcattg caggtccggg acgcccatgc ggccaccatg aacttgggcg atgaactcta
    10621 ttcgcgcgct ctgcggcgat ggacgagaat ggccgccctc ggagtggcga tttgcctgat
    10681 cggctctggc atcatgctat tgccgcggga gggaagaact agccaacgca tcggcctcta
    10741 ccgcgctgaa cgaatcgccc tgcatgatgt tcccaaaggg cgggtcggat cggccgagct
    10801 gagcatgcag gaggatcggc tcgtctggac aattgatgtc caaccaccgg cggcggccga
    10861 tttccgcgag atcgaaatcg atgcgcgcaa cggtcatgtg atcgccgccc gcaccgaatc
    10921 cgccgaagaa gaagcgctcg aagtagcggt ggaggagcat cgcccccttc cgaaaaacgt
    10981 cattccgcga tgacctctgc gctcgacggt tgcaacttgg ccaggccggc cggtttgagt
    11041 ggttgagccg gtccccggga aaaagtgccg gcgcgctggc ctaacgaatc catggctacg
    11101 gtcgggcgga gccacgcagc cagccccttt cacttccgcg ccgcgctggt tctgatcgtc
    11161 ctgggaacga ccaccggctc gatccgctcg aacccgcccc cgggctacaa cgcgtacggc
    11221 cgcaccgact cgaagtcttt tggcctaaca atcgccggct ggcggcgaat ccggcggacg
    11281 gtttcgtgga cccgatcgat gagttgcgcc gcgctgcatt ggcggagtcg ccgcgcgtag
    11341 ttttcatccc ctgaccggaa acagcccagg gtggcgccgt aggctcggcg cagttcgtta
    11401 ggcgcggcgg ggacaaggcg gcgactggaa tcgagcaagg catggcgttg cacccactcc
    11461 ggatcatcgc tcaggaccgc ggacgggacg gaac
//
LOCUS       DATMNS010000043        13691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_56284, whole genome shotgun sequence.
ACCESSION   DATMNS010000043 DATMNS010000000
VERSION     DATMNS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 13691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13691
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56284"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VNW28_01655"
     CDS             complement(<1..611)
                     /locus_tag="VNW28_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08655.1"
                     /translation="MKITFTSALLWFALLAGRSVSAADWQIVASPNLGTQANSLSSVA
                     AVADNDVWAAGWGYNQVLGAYRTVIEHWTGASWALVKSPNATNGYNLLNGVAVVAAND
                     VWAVGQAANGSTYSTLVEHWNGTAWSIVPSPNVPGLSSTLQAISVISANDIWAVGYAT
                     DSSFNNFTLTLHWNGTVWSIVLSPILNDDLLFAVDAVASNDVWA"
     gene            complement(769..1455)
                     /locus_tag="VNW28_01660"
     CDS             complement(769..1455)
                     /locus_tag="VNW28_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXA08656.1"
                     /translation="MRVLVIEDDPRLARQITRELQRYNHESSARNDGADGLQAALLDP
                     PDLVVLDLNLPGLDGLSVLARLREAHSTARILILTARGGVGDRVKGLKAGADDYLAKP
                     FSMNELIARVEALGRRAATPTAVDLLKVGDLHMDVLHRHVARAGKAIALSPREFDVLQ
                     ILMQEPGRVFSRTELCERIWQREHEYDTRTVEIFITRLRKKVDFGFDPPLIHTLRYVG
                     YAVRSPEQQA"
     gene            1702..2697
                     /gene="hprK"
                     /locus_tag="VNW28_01665"
     CDS             1702..2697
                     /gene="hprK"
                     /locus_tag="VNW28_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HXA08657.1"
                     /translation="MSSEPIIQTFCSDSKQRMTVPAITVEGFFKEHAEALQLKLESGR
                     VGFGRRIREPTINRPGLALAGFYTYFAEKRVQVLGAAEQSYLKNLSARQRNARFRALC
                     AQKIPCIVASRGFHLAPELLAIAESAGIAVFRTPMITMKFINAATIALELDFSPTLTE
                     FGSMVDILGVGVLIRGASGVGKSECVLGLIERGYSLVSDDVTRIKSIEGRELMATAPE
                     LTRYHMEVRGIGIINVVSVFGIGAIRNEKRLDLVVTLKDWPELEEVDRIGLDRELYEI
                     LKIQVPHITIPVRPGRDIARLIEVAAMDQKLKGLGVNGAVEFNEKLLDLMKTQDT"
     gene            2697..3005
                     /locus_tag="VNW28_01670"
     CDS             2697..3005
                     /locus_tag="VNW28_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXA08658.1"
                     /translation="MGLLNRRAAPTTSEKVEKEITIVNRLGLHARPAAMFVRIASRHR
                     CEIWVAKEGEEINGKSIMGLMMLAAGQGSKLRIRAEGPDAAQALIDIEELIQSRFNEE
                     "
     gene            3034..4800
                     /gene="ptsP"
                     /locus_tag="VNW28_01675"
     CDS             3034..4800
                     /gene="ptsP"
                     /locus_tag="VNW28_01675"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HXA08659.1"
                     /translation="MSGDRGHQEKRCQGVPASPGIARGPIHVFSIETDDVVKYHIEPA
                     HVPDEIARFENALIQTRVQILEMQQQIAEAIGAKDAGIFDAHLLVVEDQTLIDEVLRK
                     LEADHCNVEFVFQEVANRYVESLSRIDDPYLRERALDIQDVTRRVIRNLQGKSPRPFL
                     SVDQPHILVAHNLTPSDTATMNRDQVRGLALDLGSRTSHTAIMARSLGIPSVVGLHDA
                     TESLETGAPALMDGYHGLLIIHPSEETLREYDEIERQKGAIAKRLTQLRDTKSTTSDG
                     RHIVLSANIELPDEVDAVLAHGAEGVGLYRTEFLYLNRTTLPSEEEQFEIYRTVAQRV
                     APDPLIIRTLDLGGDKLSENNENGDELNPFLGWRAIRFCLENLEIFKAQLRAILRASV
                     NANVKMMFPMISGLDEVRRGLQIVDECKQELRREGRAFRDELEIGVMIEIPAAAIMAD
                     LLAREVDFFSVGTNDLIQYSVAIDRTNERVAHLYQPSHPSIVRLLKMVADAAHANNIW
                     VGVCGEMAGEILFTPLLLGLGMDELSAGAALVPLVKNAVQRLTMAECAQLVEQALQLD
                     TAAAILQRCTEVAQRNYPELLG"
     gene            complement(4837..5331)
                     /locus_tag="VNW28_01680"
     CDS             complement(4837..5331)
                     /locus_tag="VNW28_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HXA08660.1"
                     /translation="MNKTVIEVQVRAVLPTSGGCAVFLGNMDKVFIIYVDQSVGSAIT
                     MFMRGTVKERPLTHDLMGHLMTALGARVERVIINDLKNATYYARMIIRAENELQQKKI
                     IELDGRPSDCIAMAVQQKAQIYVSQEVWDEVDDMSDVLRKMEEDGLKPEIEPENEAES
                     GEEE"
     gene            5496..7472
                     /gene="speA"
                     /locus_tag="VNW28_01685"
     CDS             5496..7472
                     /gene="speA"
                     /locus_tag="VNW28_01685"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HXA08661.1"
                     /translation="MTKPEWDIDAAIATYNVEGWGDGYFSVNSTGNVCAKPLKDAGGS
                     IDLLEVVNEARERGLGFPLVIRFQDLLRHRVETVNRAFQAAMQEFGYRNAYRGVFPIK
                     VNQLREVIEEIVDAGAPFHFGLEAGSKPELIAALAMHQDLESLIICNGYKDRAFIRTA
                     MLGRRLGKSVIIVVEKLEELEQTLRIAKEMGVEPFIGIRVRLHSKGAGKWTTSGGENA
                     KFGLSTTNLVTASELLKAHGLAHCLKLVHFHVGSQVPDISTIKRAVREAARYYAKLSK
                     MGHELGYLDVGGGLGVDYDGSRTSFDSSTNYSLQEYCNDVVYNIMEVCDSEGVVHPAI
                     VSEGGRAIVAHHSVLVVEAFGSIEKDMHAPKIEATPGDPKPLRDIIEVKRQLKRGNRL
                     ETLHDAQQIKEEMQQMFNLGLLDLEAKAKIEALYWHLARQIVNLHRGLKYVPEEVKEL
                     ETSLADQYICNFSVFQSLLDHWALGQLFPIMPIHRLATPPERSGTLVDITCDSDGKIN
                     KFTDLQDVKDTLPMHKITPGELYYLGVFLVGAYQDIMGDLHNLFGRVTEVHVFLDEDE
                     ESGWYIEEVIEGSTIGQVLALTQWDKIELMRLVKLQVDAAIKSDRLKPNDAMKILADY
                     ERGLQGYTYLSLDGAAAANGAIPAPVQLAATSPG"
     gene            complement(7495..9246)
                     /locus_tag="VNW28_01690"
     CDS             complement(7495..9246)
                     /locus_tag="VNW28_01690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXA08662.1"
                     /translation="MGFRLILFVVALALLPDRLAAQSAPAFSKVILFGDSLSDDGNIK
                     HVVQDKFFISYPSLAFDYSDGRFTNSATTFPGSNTYAGTWHEQLAQTFLGLSAASNSL
                     DGGNDYAFGGATTVSGTSERTVISNPAPFIGGEYTITIDNLGKQVDDYLGSHTPDPAA
                     LYIVWGGGNDLFDDDSSANVTATAQRVAGLVEQLARAGAVNILVPNVPPLGLIPNYEN
                     DVAQATALNAASAAYREEFNRQLDTAISTLAGESITISLYRLDIYGLFYRLAANPADY
                     GFVNISDSAQFQAVNPDQYLFWDDIHPTTAGHHQIAAAAFDLLNGTALPPAQSLNLSA
                     RLPVGTGDNVLIGGLIIAGTEAKQVIVRGLGPSLAVNGIPLGGTLADPTLELYQGSTL
                     IMSNDNWKDSQEGAIEATGIPPTDDLESAIVASLAPGTYTVILQGKNNGTGIGLVEAY
                     DLDPTAQSTLANTSTRGLVQTGDNVLIGGFIVGNGGSDTVVVRAIGPSLAAAGVANPL
                     ADPTLDLYDGNGVLILSDDNWRDSQEALIQSTGLAPKNDAESAIIRSLTPGAYTAVVR
                     GKDGATGVALVEVYNLQ"
     gene            complement(9359..11041)
                     /locus_tag="VNW28_01695"
     CDS             complement(9359..11041)
                     /locus_tag="VNW28_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXA08663.1"
                     /translation="MLQMQDMMSKSMPDFKEGSIVKGRILEIRPREVLVDIGYKSEGV
                     IPTAEFDDIESLEVGDEVEVLLERLENDEGMVVLSKEKAAYRQNWNKIAQVFAGDGLI
                     KGKVKSVVKGGLMVNIGVEAFLPASQIDIIPPRDLQQFVGNTYDFKIVKINDDRKNVV
                     LSRRELIEQERSEKRQKFLEGVNVGDTVSGAVKNLTDFGAFIDLGGMDGLLHITDMTW
                     GRLGHPSELLKVGQQLEVLVLDINKEKERVSLGLKQTQKNPWDQIEERFPAGQKVKGK
                     ITNLVPYGAFVELEEGVEGLIHVSELSWTKRIMRPSDILTVGQEVEAVVLGVNKEEQK
                     ISLGLRQLEPNPWDEIEKKFTIGSRVKGQIRNMTAYGAFVELEDGIDGMIHVSDLSWT
                     RKINHPMEVFKKNDEVEAEVIDIDKTNQRISLGVKQLSDDPWKNIDEKYKIGDLVTGK
                     VTKLASFGAFVQLQDDIDGLVHISQLSEDHVAKVKDVLKVGQEVEARVIKVDKVERRI
                     GLSIKAANYSEEELRRESEAFDNLRPGEDMVGLEKAFEAAEEDYRPGEGKKK"
     gene            11209..11547
                     /locus_tag="VNW28_01700"
     CDS             11209..11547
                     /locus_tag="VNW28_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08664.1"
                     /translation="MSWSTSEAMTDPAIREPMSFRSEFKFFLRDIPVEITVRLYKPIH
                     ASAVVVRRSHLISVPGLDRPEQVSDEDQMEDGEVLQSVISEFVHVYNAAVAKGLKPDV
                     SWLQQDPGFR"
     gene            11568..12053
                     /locus_tag="VNW28_01705"
     CDS             11568..12053
                     /locus_tag="VNW28_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXA08665.1"
                     /translation="MKRLCILFLTAACFSSPLQAASPAETQVADAIKAPGLTVVHLWA
                     PWCSNCQAELKGGGWTKTVKDNPGVKFYFISVWNGGEDGRAVLAKYEIAQQPNVTILA
                     DPGPRGAGHIKQFTGLALSWIPTTWIFKGGDLRYALNYGELRFSVLQQFIADTNSDWE
                     H"
     gene            12145..12876
                     /gene="rpsB"
                     /locus_tag="VNW28_01710"
     CDS             12145..12876
                     /gene="rpsB"
                     /locus_tag="VNW28_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXA08666.1"
                     /translation="MSNTIELVPELLEAGVHFGHQTKRWNPKMKPFIFEKRNAIYIIN
                     LDETVKQLQRATDFLREVAQRGGKILFVGCKKQAQEAVKEAAVACGQFYVNQRWLGGT
                     LTNLATIRKSIGRLKYIEEIEASPDYKKMGKQELAALNREAAKLRRNLEGILEMSQLP
                     DALIIIDTTREQNAVNEARRLGILTVAIVDTNADPEIIDYPIPGNDDAIRAIRVMLQK
                     LVDAIVSASGEARLREEVEMAGVSA"
     gene            12978..13607
                     /gene="tsf"
                     /locus_tag="VNW28_01715"
     CDS             12978..13607
                     /gene="tsf"
                     /locus_tag="VNW28_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXA08667.1"
                     /translation="MEINPQTVKQLREKTNAGFMDCKRALAEAGGDLDKAETLLRTKG
                     IASASKKSSRATKEGIVASYIHLQGKVGVLVEVNCETDFVAKNEGFRAFVKDITLHIA
                     AAQPLYVGREEVPPATIDAERALYAAQVKGKPENVMAKIVEGKLDKFYSTVCLLEQGF
                     IKNPDQTIKELVASKIAEIGENIVIRRFTRYSVGEPLSHEPSIPSAAEE"
BASE COUNT         2851 a         4071 c         4022 g         2747 t
ORIGIN      
        1 gcccagacat catttgaagc gaccgcatcc actgcaaaca ggaggtcatc gttcaggatc
       61 gggctgagca cgatgctcca gacggtgcca ttccagtgca gcgtaagagt gaagttgttg
      121 aaactgctgt cggtggcgta accaaccgcc cagatgtcgt tggccgagat gacagagatc
      181 gcctgcaagg tgctggataa tccgggaaca ttagggctgg gaacaatgct ccacgccgtc
      241 ccattccaat gttccaccaa agtgctataa gtgctgccgt tcgcggcctg gccgacagcc
      301 caaacatcgt tcgcggccac gacggcgaca ccattgagga gattataacc gttggtcgcg
      361 ttcggacttt tcacgagcgc ccagctggcg ccggtccaat gttcgatcac ggtgcggtaa
      421 gcgcccagca cctgattata gccccatccg gccgcccaaa catcattgtc ggccaccgcg
      481 gccaccgacg ataaactgtt ggcctgggtg cccaaatttg gactggcgac aatctgccaa
      541 tcagccgccg agactgaacg gccagcgagc agagcaaacc aaagaagagc ggaggtaaac
      601 gtgattttca cgtgccgaga gtaggcgcaa gacattgcct cacaacaagc tctccgtaac
      661 aggtttgtta cgttcagaac aacctccagg agacgaacag cctaaatcag caatccacga
      721 gacaggcccg cgcaaagagc ccggaaactc tgggtccatt ctcctgcact acgcctgctg
      781 ctccggcgac cggacggcgt aaccaacata gcgcaacgta tggataaggg gcggatcgaa
      841 tccgaaatcc actttcttac gcagacgagt aatgaaaatc tccaccgtgc gcgtgtcgta
      901 ctcatgttcg cgctgccaga tccgttcgca aagctccgtg cgggaaaaga cgcgtcccgg
      961 ctcctgcatg agaatttgca ggacgtcgaa ttcacgcggc gagagcgcga tcgccttccc
     1021 ggcgcgcgcg acgtgccgat gcagcacatc catgtgcaaa tcaccgacct tgaggaggtc
     1081 caccgcggtc ggagtcgccg cgcggcgtcc gagcgcttcg acccgcgcga tcagttcgtt
     1141 catcgagaac ggcttcgcca agtagtcatc ggcacccgcc ttgagacctt tcactcgatc
     1201 accgaccccg ccccgagccg tgagaatgag gattcgcgcc gtggaatgag cttcgcgcag
     1261 gcgagccagc accgataacc cgtcgagccc gggaagattg agatcgagca ccacgagatc
     1321 aggcggatcg agcagggcag cctgtaagcc gtcggcccca tcgttgcgtg cgctcgactc
     1381 atgattatag cgctgcagct ctcgagtgat ttgccgggcc aaccgaggat cgtcttcgat
     1441 aactaggacg cgcattccat ttcagcagga acctgcataa cgatgaacga acgagaaaag
     1501 cgagctaatt ctgcaattcc aaggttacag ccgtcgctgg cggcccaaat tggtttatct
     1561 gccggaagac aacgccgcga ccaaaacgcc ccgcggccgg cccgcaatct ttcgcgcaac
     1621 ggcctcgcgg ttccgagggg tgtcgagcgc tgaaggacgc tctggcaatc gcccccaatt
     1681 cggaatacaa ctggcttgcg catgagttcg gaaccgatca ttcagacttt ttgttcggac
     1741 agcaaacagc gcatgactgt ccccgccatc acggtggagg gtttcttcaa agaacacgcc
     1801 gaggcactgc aactcaagct cgaaagcggc cgcgtcgggt tcgggcggcg catccgcgag
     1861 ccaaccatca atcgtcccgg actcgcgctc gccgggttct acacttattt cgcggaaaag
     1921 agggtgcagg tgctcggcgc cgcggagcaa tcctacctca agaacctctc ggcgcggcag
     1981 cgtaacgcgc gtttccgcgc gctttgcgcc caaaaaatac cctgcatcgt cgcttcgcgc
     2041 ggtttccatc tcgcgccgga gctgctagcc atcgccgagt cggccggaat cgcggttttc
     2101 cgcacgccga tgatcacgat gaagttcatc aacgcggcga ccatcgcgct ggagctcgat
     2161 ttttcgccga ccctgacgga attcggcagc atggtggata ttcttggcgt cggcgtgttg
     2221 atccggggcg cgagcggggt cggaaaaagc gagtgcgtcc tgggcctgat cgaacgcggc
     2281 tacagtctcg tctcggacga cgtcacgcgg atcaagtcga tcgagggccg cgagttgatg
     2341 gcgacggctc cggagttgac ccgctaccac atggaggtgc ggggcatcgg catcatcaac
     2401 gtcgtttccg tcttcggcat cggcgccatc cggaacgaaa aacggctcga cctcgtggtc
     2461 acgttaaaag actggccgga gctggaagaa gtggatagaa tcgggctcga tcgcgagctt
     2521 tatgagattc tgaagatcca ggtcccgcac atcacgattc cggtgcggcc gggacgagat
     2581 atcgcgcgac taatcgaggt ggcggcgatg gatcagaagc tgaaaggcct cggcgtgaat
     2641 ggcgcggtgg aatttaacga gaaattgctg gatctaatga agacgcagga tacctgatgg
     2701 gactattaaa tcgacgcgca gcgccaacca cgagcgaaaa ggtcgagaaa gagatcacga
     2761 tcgtgaatcg gctcggtctc catgcccggc ccgcggcgat gttcgtccgg atcgcgagcc
     2821 gccatcggtg cgagatctgg gtcgcgaagg aaggcgagga aattaacgga aaaagcatca
     2881 tgggcctgat gatgctcgcc gccggtcagg gctcgaaatt gcgcatccgg gccgaagggc
     2941 ccgatgccgc ccaggcgctc atcgacatcg aagaattaat tcagtcgcgc ttcaacgaag
     3001 aatagttcac gttccggtta ttcttctacc ggaatgagcg gcgatcgcgg ccaccaggaa
     3061 aagcgctgtc agggtgtgcc cgcgtccccg gggatcgcgc gcgggccgat ccacgtcttc
     3121 tcgattgaaa ccgatgacgt ggtcaaatac catatcgagc cggcgcacgt gcccgacgag
     3181 atcgcgcgct tcgagaacgc gctcattcag acgcgggtgc aaatcctcga gatgcagcag
     3241 cagattgcgg aggcgatcgg agcgaaggat gcgggcattt tcgatgccca tcttttggtc
     3301 gtcgaagacc agaccctgat cgacgaagtg ctgcgcaaac tcgaggccga tcactgcaac
     3361 gtcgagttcg ttttccagga agtcgccaac cgctatgtcg aatcgctcag tcgcatcgac
     3421 gatccttacc tgcgcgagcg cgcgctcgac atccaggacg tcacccggcg cgtcatccgc
     3481 aatttgcagg gtaaatcgcc gcgtccgttc ctttcggtcg accagcccca cattctggtc
     3541 gcgcataatc tcaccccatc cgataccgcg acgatgaacc gcgaccaggt gcgcggcctt
     3601 gcgctcgatc tggggagccg cacctcgcac accgcgatca tggccaggtc gttgggcatt
     3661 ccgtccgtcg tcggcctgca cgacgcgacg gagagcctcg agacgggcgc gcccgccctg
     3721 atggatggct accatggcct gctcatcatc catccgagcg aagagacgct gcgcgaatac
     3781 gacgagatcg aacggcaaaa aggggcgatc gcaaaacggc tgacgcagct gcgcgacacc
     3841 aaatcaacca cctccgatgg ccgccacatc gtgctctcgg cgaacattga actgcccgac
     3901 gaagtggatg ccgtcctcgc ccacggtgcc gagggcgtgg gcctttaccg gaccgagttt
     3961 ctttatctca accggacgac tttgccctcc gaggaagagc agttcgaaat ttatcggacc
     4021 gtcgcccagc gggtcgcgcc cgacccgttg atcattcgca ccctcgatct gggcggtgac
     4081 aaactttccg agaacaacga gaatggagac gaactcaacc cctttctcgg ctggcgggcc
     4141 atccgtttct gcctcgagaa cctggaaatc ttcaaggcgc agctccgtgc catcctgcga
     4201 gccagcgtga acgcgaacgt gaagatgatg tttccaatga tctcgggcct cgatgaagtg
     4261 cggcgcggcc tgcagatcgt cgacgaatgc aagcaggagc tgcggcggga agggcgtgcc
     4321 tttcgcgatg agctggaaat tggcgtcatg atcgagatcc cggccgcggc gatcatggcc
     4381 gatctgctcg cgcgcgaagt ggatttcttc agcgtcggga cgaacgacct gatccaatac
     4441 tccgtcgcga tcgatcggac gaatgagcgc gtggcccatc tttaccagcc gagccatccg
     4501 tcgatcgtga ggctgcttaa aatggtggcg gatgccgcgc acgcgaacaa catctgggtg
     4561 ggcgtctgcg gcgaaatggc aggcgagatt ttgtttacgc cgctcctcct cggcctcggg
     4621 atggacgaac tgagcgcggg cgccgcgctc gtcccgctag taaaaaacgc ggtccagcgc
     4681 ctaacaatgg cggaatgcgc gcaactggtc gagcaggccc tccaactcga taccgccgcg
     4741 gcgatcctcc agcgctgcac cgaagtggcc caacgaaatt atcccgagct gctcggctag
     4801 gcggcgacag gatcggtcgt gagacggacg agcggtttat tcttcctcgc cggattccgc
     4861 ttcattctcc ggttcgatct ccggcttgag gccgtcctct tccatcttgc gcaggacgtc
     4921 gctcatgtcg tcgacttcgt cccagacttc ctggctgacg tagatctgcg ccttttgctg
     4981 caccgccatc gcgatgcaat cgctcgggcg accgtcgagc tcgatgattt ttttctgctg
     5041 cagctcgttc tcggcccgaa taatcatccg cgcgtagtag gtcgcgttct tcaggtcgtt
     5101 gatgatcacc cgctcgacgc gcgcccccag ggcggtcatc aggtgtccca tcagatcgtg
     5161 cgtcaagggg cgttccttga cggtcccgcg catgaacatc gtgatcgcgc tcccgacgct
     5221 ctggtcgaca tagatgatga agaccttgtc catattgccg aggaagaccg cgcagccgcc
     5281 gcttgtcggc aggacggcac gcacctgcac ctcgatcacc gttttgttca tcaaaaaaac
     5341 cgtatcagag cgatttcgaa aaacaagcgg gccggcggca ctcgcaagtc gcggcgcgtt
     5401 ggctctccgc cttctttctt cctctggcga cagtcttttt ctatcggcta atctgcctgt
     5461 tgagtttgca atctggtgcc ctacaatgcg ccacgatgac gaagcccgag tgggatatag
     5521 acgccgcaat cgcaacttat aacgttgaag gttggggcga cggttatttc tccgtcaaca
     5581 gcaccggcaa cgtctgcgca aagccattaa aggacgccgg cggctcgatc gatttgctcg
     5641 aagtggtgaa tgaagcgcgt gagcgcggcc tcggctttcc gcttgtcatc cggttccagg
     5701 atttgctccg gcacagggtg gagacggtca atcgcgcttt ccaagcggcg atgcaggagt
     5761 ttggttatcg caacgcatat cgcggggtgt ttccgatcaa ggtcaatcag ttgcgcgagg
     5821 tgatcgagga gatcgtcgat gccggggcgc cgtttcattt cgggctcgaa gcgggaagca
     5881 aaccggagtt gatcgcggcc ctcgcgatgc accaggatct cgagagcctg attatttgca
     5941 acggttacaa ggatcgcgct ttcattcgca ccgcgatgct cggccgccgg ctcggcaagt
     6001 cggtcattat cgtcgtcgaa aaactcgaag agctggagca gacgctgcgc atcgcgaagg
     6061 agatgggcgt cgagcctttt atcggcatcc gcgtgcggct gcacagcaaa ggcgccggaa
     6121 aatggacgac gagcggcggc gagaatgcga aatttggcct gagcacgacc aacctcgtca
     6181 cggccagtga attgctgaaa gcgcacgggc tcgcgcattg tctcaagctc gtccacttcc
     6241 acgtcggctc gcaggtgccc gacatttcca cgatcaagcg cgccgtccgc gaagcggcgc
     6301 gctattacgc gaaactgtcg aagatgggtc acgagcttgg ctacctcgat gtcggcggcg
     6361 gcctcggggt cgattacgat ggctcgcgca ccagcttcga cagctcgacg aattactcgc
     6421 tgcaggagta ttgcaacgac gtcgtttaca acatcatgga ggtctgcgac tccgaaggtg
     6481 tcgtccatcc cgcgatcgtg agcgaaggcg gccgcgcgat cgttgctcat cactctgttc
     6541 tcgtggtcga ggcgtttggc tcgatcgaga aagacatgca cgcgccgaaa atcgaggcca
     6601 caccgggcga tccgaaaccg ctgcgtgaca tcatcgaggt gaaacgccag ctgaagcgcg
     6661 gcaaccggct cgaaacattg cacgacgcgc agcagatcaa ggaagagatg caacaaatgt
     6721 tcaatctcgg gctgctcgat ctcgaggcga aagccaagat cgaggcgctc tattggcacc
     6781 tcgcgcgcca aatcgtcaac ctgcaccgcg gcctgaaata tgtgcccgag gaagtgaagg
     6841 aactcgagac gtcgttagcc gatcagtaca tctgcaactt ctcggtcttt caatctctcc
     6901 tcgaccactg ggcgctcggc cagcttttcc cgatcatgcc gatccatcgt ctcgccacac
     6961 cgcccgagcg ctccggaacg ctggtggaca tcacctgcga ttccgacggg aagattaaca
     7021 agtttaccga cttgcaggat gtgaaggaca cgctgccgat gcacaagatc accccgggag
     7081 aactctatta tctcggtgtt ttcctggtcg gcgcctacca ggacatcatg ggcgacctgc
     7141 acaatttgtt tggccgcgtg acagaagtgc acgtcttcct cgacgaagat gaggagtcgg
     7201 gttggtacat cgaagaggtg atcgaaggaa gcacgatcgg gcaggtcctg gcgctcacgc
     7261 aatgggacaa gatcgagttg atgcgtctgg tgaaactgca ggtggatgcc gcgatcaaga
     7321 gcgatcggct caagcctaac gacgcgatga aaatcctggc cgattacgag cgcgggttgc
     7381 agggttacac ctacctctcg ctcgatggcg cggcggcggc gaatggcgcc attcctgccc
     7441 cggtgcagct ggcggcgact tcgccgggct aacctttggg ccggcgcgag ccggttattg
     7501 cagattgtaa acttcgacca gcgccacgcc cgtagcgccg tcctttccgc ggaccacggc
     7561 ggtgtaggcc ccgggcgtga gcgagcggat gatcgccgat tcggcgtcgt tcttcggcgc
     7621 gaggccggtg gactggatga gcgcctcctg cgagtcgcgc cagttgtcgt cgctcagaat
     7681 caggacgccg ttgccatcgt aaagatcgag tgttggatcg gccagcggat tggcgacacc
     7741 cgccgcggcc agtgatggtc cgattgccct aacgaccacc gtgtcactgc cgccgttgcc
     7801 gacaatgaag ccgccgatca aaacattgtc acccgtctgg accagcccgc gcgtgctcgt
     7861 gttggcaaga gtggattgag ccgtgggatc gagatcgtag gcttccacca gcccgatgcc
     7921 ggtgccgtta ttttttccct gaaggatgac cgtgtaggtg cccggcgcga ggctggcgac
     7981 gatcgcggat tcaagatcgt cggtcggcgg aatgccagtc gcttcgatcg cgccctcctg
     8041 cgagtctttc caattgtcgt tgctcatgat gagcgtgctg ccctgataaa gttccagggt
     8101 cggatccgcc agcgtgccgc cgagcggaat gccattgacc gcgagcgatg gaccgagccc
     8161 cctaacgatg acctgttttg cttcagtccc ggcaataatg agaccgccga tcaggacatt
     8221 gtcgcccgtg ccgacgggaa ggcgcgccga gaggttgagc gactgggccg gaggcagcgc
     8281 ggtgccattc aacaaatcga aggccgcggc cgcgatctga tgatgcccgg ccgtcgtcgg
     8341 atgaatgtcg tcccagaaaa ggtattggtc cggattgacc gcttggaatt gcgcgctgtc
     8401 gctgatgttg acgaatccat agtccgccgg gttcgcagcc agccggtaaa acagtccgta
     8461 aatatcgagc cggtaaagcg agatcgtgat ggactcgccc gcaagcgtgc tgatggcagt
     8521 gtcgagctga cggttgaatt cctcgcgata agcggctgaa gccgcattca gcgcggtggc
     8581 ctgcgccacg tcgttttcat aattcggaat gagcccgagc ggcggaacgt tgggcaccag
     8641 gatattgacg gctcccgcgc gcgcgagttg ttctaccaaa ccggccacgc gctgggcggt
     8701 ggcggtgaca ttggcggaac tatcgtcgtc aaaaagatcg ttcccgccgc cccagacgat
     8761 gtagagcgcc gccggatcgg gcgtgtgact gccgagataa tcgtccacct gtttaccgag
     8821 gttatcgatc gtgatcgtgt attcgccgcc gatgaacggg gcgggattcg aaatgaccgt
     8881 ccgctcgctc gtgccgctca cggtcgtcgc gccaccgaag gcgtaatcgt tgccgccatc
     8941 aaggctgtta ctggccgccg acaagccgag gaaggtctgg gcaagttgtt cgtgccaggt
     9001 gccggcatag gtgttggagc ctggaaacgt agtggcgcta ttggtgaagc gaccgtcgct
     9061 gtaatcgaac gccaggctgg ggtagctgat gaagaacttg tcctggacca cgtgcttgat
     9121 gttgccatca tccgacaggc tgtcgccgaa gaggatgact ttactgaacg ccggcgcgct
     9181 ttgagccgcg agccggtcgg gtaaaagcgc cagagcgacg acgaaaagga taaggcgaaa
     9241 gcccatgtag acgcttcgcg gtgcgaaacg cgggtgtcga ggtggaatgt gctgcctcaa
     9301 gccgagcctg gtcctcacgc ttcgcagagc gagacgtcta ccgcacaaaa cgtcttcgct
     9361 actttttctt gccctcgccg ggacgataat cttcctcggc cgcctcgaac gccttctcga
     9421 gcccgaccat atcttcgccg ggacgcaggt tatcgaacgc ttcggattca cgccgcagct
     9481 cttcctcgga ataattagcg gccttgatcg agagaccgat gcggcgctcg accttgtcga
     9541 ccttgatgac gcgcgcttcg acctcctggc cgaccttgag cacatccttc actttcgcga
     9601 cgtgatcttc actcagttgc gagatgtgga cgaggccatc gatgtcgtcc tgcaattgca
     9661 caaaggcgcc gaagctggcc agcttcgtca ccttgccagt aacgagatcg ccgatcttgt
     9721 acttctcgtc gatgttcttc cagggatcgt cgcttagctg tttaacgccg aggctgattc
     9781 gctggttggt cttgtcgatg tcgatcactt ccgcctcgac ttcgtcgttc tttttgaaga
     9841 cctccatcgg gtgattgatc ttgcgcgtcc agctcaaatc cgagacgtga atcatgccgt
     9901 cgatcccgtc ttccagttcc acgaacgcgc cataagcagt catgttgcga atctggcctt
     9961 tcacccggct gccgatggtg aatttcttct cgatctcatc ccatggattg ggctcgagct
    10021 gacgcaggcc taacgaaatt ttctgctctt ccttgttgac gccgagcaca acggcctcca
    10081 cttcctggcc aacggtgagg atgtccgacg gacgcatgat gcgcttcgtc cacgataact
    10141 cggagacgtg aatgaggcct tcgacgcctt cttccaattc cacgaaagcg ccatacggaa
    10201 cgagattggt gattttgcct ttgaccttct gcccggccgg gaaacgctct tcgatctggt
    10261 cccacgggtt tttctgggtc tgtttcaggc ctaacgaaac acgttctttt tccttgttga
    10321 tatcgaggac gagcacttcg agttgctggc caactttgag caattcgctc gggtggccga
    10381 gccggcccca agtcatgtca gtaatatgca aaaggccgtc catcccgccg aggtcgataa
    10441 acgcgccaaa atcggtgagg ttcttgaccg cgcccgacac agtgtcgccc acgttgacgc
    10501 cctcgaggaa tttttgacgc ttctcgctcc gctcctgctc aatcaattcg cgccggctca
    10561 gaacgacgtt ttttcggtcg tcgttgatct taacgatctt aaaatcgtag gtgttcccga
    10621 caaactgctg gagatcgcgc ggcgggatga tgtcaatctg ggacgctgga aggaaagctt
    10681 cgacgccgat attgaccatc aggccgccct tcacgaccga cttcactttg cctttgatca
    10741 atccgtcgcc ggcgaagacc tgcgcaatct tgttccaatt ctggcgataa gcagcctttt
    10801 ccttcgaaag gacgaccatg ccctcgtcgt tctcgaggcg ctcgagcagc acttccacct
    10861 cgtcgcccac ctcgagcgac tcgatgtcgt cgaactccgc cgtcgggatc acgccctccg
    10921 acttgtaacc gatatccacg agcacttcgc gtggccggat ttccagaatg cgccctttca
    10981 caatgctgcc ttctttgaag tcgggcatcg acttcgacat catatcctgc atttgcaaca
    11041 taaacggtgg gctctcctgg tgacgcgggt cagtgttaaa atcagcggcg actatcgccg
    11101 gctgctcccg cacgaaggga tggaaagtag ccacgttccg gcccgcggtc aaccggcgcc
    11161 agaaggggat tgatcccggg tgtgaccccg tttagcttcg ccccgtctat gagttggagc
    11221 accagtgaag ccatgaccga cccggcgatt cgcgagccga tgtcttttcg ttcggagttt
    11281 aagttcttcc tgcgggatat tcccgtcgag atcaccgtgc gcttatacaa gcctattcat
    11341 gcgagcgctg tcgtcgtccg gcgaagccat ctgatatcgg tcccgggttt ggaccggccg
    11401 gagcaggtta gcgacgagga tcagatggaa gacggtgaag tcctccagag cgttatcagc
    11461 gaattcgttc acgtctacaa tgcggctgtc gccaagggct tgaagcccga cgtctcctgg
    11521 ctacagcaag atcctgggtt tcgctgaaat cgcctgatta actcctgatg aaaagacttt
    11581 gcatcctctt tttgactgcg gcctgctttt cgtctcccct gcaggcggcg tcaccagccg
    11641 aaacgcaagt cgccgatgcg atcaaagcac cgggtcttac ggtcgttcac ctctgggcgc
    11701 cgtggtgctc caactgccag gctgaactta agggcggcgg ctggacgaag accgtgaagg
    11761 acaatcccgg ggtaaaattc tatttcatct ccgtctggaa cggcggcgaa gacggccgcg
    11821 ccgtgctcgc gaagtacgag atcgcgcagc agcctaacgt caccattctg gccgatcccg
    11881 gcccgcgggg cgccggccat atcaagcagt ttaccggtct ggccttgagt tggattccca
    11941 ccacctggat cttcaaaggc ggcgaccttc gctacgcctt aaactacggg gaactccgtt
    12001 tttctgtcct gcaacaattc atcgccgata ccaactcgga ttgggaacac tgacgctttc
    12061 ccgggccaaa ttgaagttgc gggagcaagg tgggtgtagc atcttccccg tccgctggac
    12121 cggtgcggcc ggcgcctctt tactatgtca aatacgatcg aactagtgcc cgagctgctc
    12181 gaagcgggcg tccattttgg ccaccagacg aagcgctgga atccgaaaat gaagccgttc
    12241 atttttgaga agcgcaacgc gatttacatc atcaacctcg atgaaacggt caaacagctc
    12301 cagcgggcga ccgatttcct gcgcgaagtg gcgcagcgcg gtgggaagat tcttttcgtc
    12361 ggctgcaaga aacaggccca ggaagcagtc aaggaagcag ccgtagcctg cgggcaattt
    12421 tacgtcaacc agcgttggct cggcggtacc ctgacgaacc tggccacgat ccggaaaagc
    12481 atcggccgcc tgaagtacat cgaggagatc gaagcctcgc ccgattacaa gaaaatgggc
    12541 aaacaggagc ttgcggcctt gaatcgtgag gctgccaaac tgcgtcgcaa cctcgaaggc
    12601 attctcgaaa tgtcgcaact ccccgacgcg ttgatcatca tcgacaccac gcgcgagcag
    12661 aatgccgtca acgaagcacg ccggcttggc atcctgactg tcgcgatcgt cgatacgaac
    12721 gccgacccgg agattatcga ctacccgatt cccgggaacg acgacgccat ccgggccatc
    12781 cgcgtcatgc tgcaaaagct cgtcgacgcc atcgtctcgg ccagcggcga agcgcgcttg
    12841 cgcgaggaag tcgagatggc tggcgtctcc gcctaacggc cgcggttgag agtcggtagt
    12901 tgagagttga gaggctccgc tatcggagtt ctctctcggc ttcaatttca atccctttca
    12961 gttctcaaat ctcagctatg gaaataaatc ctcagaccgt caaacagctc cgcgaaaaaa
    13021 cgaacgcggg cttcatggat tgcaaacgcg cgttggccga agcgggtggc gacctcgaca
    13081 aagcggagac gctcctgcgg accaagggaa tcgccagcgc gagcaagaag tcttcgcggg
    13141 cgacgaagga gggcatcgtc gcttcctaca ttcatctcca gggaaaagtc ggcgtgctgg
    13201 tcgaggtaaa ctgcgagacc gatttcgtgg cgaagaacga aggctttcgc gcctttgtga
    13261 aggacatcac gctgcatatc gccgctgcgc agccgcttta tgtcggccgc gaggaagttc
    13321 cgccagccac gatcgacgcc gagcgcgcgc tctacgccgc ccaggtgaaa gggaaacccg
    13381 agaatgtgat ggcaaaaatt gtcgaaggga aactcgataa attttacagc acggtctgcc
    13441 tgctcgagca gggtttcatc aagaatcccg accagacgat caaggaatta gtcgcctcga
    13501 agatcgccga gatcggcgag aacatcgtga ttcgtcgttt cacccgctac tcggtgggtg
    13561 agccgctgag ccacgagccg tcaattccat ccgcggcgga agaatagcct gcgtggtcgg
    13621 cctaacaagg ccagtcggta tgcatcgtgt tttggggctg cggctcaatt gacgagaaac
    13681 agagcgcccc g
//
LOCUS       DATMNS010000044         4576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5064384, whole genome shotgun sequence.
ACCESSION   DATMNS010000044 DATMNS010000000
VERSION     DATMNS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5064384"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VNW28_01720"
     CDS             complement(<1..1596)
                     /locus_tag="VNW28_01720"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HXA08668.1"
                     /translation="MTSNPNPANRVANPPSKMLMVYDGDCGFCKLWIARWREITAGAV
                     DYESLQEAAARFPEVPRAEFERAVKLIEPDGRVLSGAAAVYRSLGRGGGKKFWSWSYE
                     HVPGVAPVSKAAYAFIAGHRELAHIVTSLFWGKDVRRPTYFHARRWFLRMLGMIYLIA
                     FWSLWVQVDGLIGAHGVMPISQLLGDAHKQLGSRAFFLLPTFCWLNPSNAFLHLLCGA
                     GALLSVLLIVEIAPIVCLALLFALYLSLTIAGQTFLSFQWDILLLETGFLAIFLAPWR
                     WWPRRDSFAPLSRPALFLLHFLLFKLMLMSGVVKLTSGDASWWDLSALDFHYWTQPLP
                     TLLGWWADKSPEWFKHFSTAFVLVVEIAGAFLVWLPRRLRLLGCGAIIFLQIVIGLTG
                     NYAFFNLLTIALCLLLIDDLVWPGGPRLTNRAPAGRGWPVWVPAVIIVLTMPVNALLI
                     FSGFKPGTEWPGPMGAMYGVIAPFYVVNGYGLFRVMTKERSEIILEGSDDGLEWKPYE
                     FKWKPGALNRPPGFVEPHQPRLDW"
     gene            complement(1679..2449)
                     /gene="xth"
                     /locus_tag="VNW28_01725"
     CDS             complement(1679..2449)
                     /gene="xth"
                     /locus_tag="VNW28_01725"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HXA08669.1"
                     /translation="MRIVSWNINSLRKRQDRLLLWLRETNPDVVCLQETKCTDEQFPQ
                     KALRDAGYAAVFHGQKSYNGVAILAQEEPREVRIGFCDEVADEQARVIAASVGGVRVV
                     SIYAPNGQAIGAPAYDYKLAWYERLTKCLREKEGAIERIVVGGDYNVAPNDADVHDPA
                     LWRGAIMCSEKERAAFRALCAVGLADTLRLRHPEAGIFSWWDYRMLAFPKNRGLRIDA
                     VLAGRELAAKCTAAGVDREMRKGKDPSDHAPVWAEFAV"
     gene            complement(2579..3952)
                     /locus_tag="VNW28_01730"
     CDS             complement(2579..3952)
                     /locus_tag="VNW28_01730"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXA08670.1"
                     /translation="MKFINLTRRTEIGANCYYLGVGGQGVVLDCGMHPKEEGEMALPL
                     LGTLAGRTVDALLLSHAHLDHVGSLPVLMRQQPQARIFMTEPTAAIGEALLHNSVNVM
                     TREREEVGVIYPLFTHRETERATDRWQHCPVGQPFTVDGERSPARPNDELSFEFYDAG
                     HVLGAAGILLRAEGRTVFYTGDVNFDDLTLLKGAVFPESGIDVLIIEATRGDSPLAKG
                     FSRDAEERRFAAAIERAFARGGCVLVPVFALGKTQEVLSMFYKFKRNKMIADVPIYIG
                     GLSSKMTEIYDRRANQSRRVLPRLQLFPEVGPFVLNGKTIADAPARPGRIYALSSGMM
                     TPKTLSNIFARRIIDRPEHSIFFVGYADPESPAGVLRRTAPNEFLSLDEEEPAQPLRC
                     RVEEFQFSAHASRESILAYITKVAPKKVVLVHGDGPAVEWVRASAAVALPGSEIIVPP
                     PGVEIEL"
     gene            complement(4038..>4576)
                     /locus_tag="VNW28_01735"
     CDS             complement(4038..>4576)
                     /locus_tag="VNW28_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08671.1"
                     /translation="FGESNGMWTYYRTPNSDALAEEAIDKCRYARFIPAVYHGKPAAA
                     VVSGTMIFVVIDGKPHLRIFLNQEAEHLKKGDDFICPQEVFWWGDNFEGFAYPSDHML
                     SGTVCVKLDTDATGKLLASKITYESPLGSGFGKAVMDRINKVTFLPGYLRGKPVACST
                     TSQILFRGRGTNTYWKTD"
BASE COUNT          963 a         1470 c         1358 g          785 t
ORIGIN      
        1 ccaatcgagc cgcggctggt gcggctcgac gaaacccggc ggccgattga gcgcgcccgg
       61 tttccatttg aattcgtagg gtttccattc gaggccgtcg tcgctcccct cgaggatgat
      121 ctccgaccgc tccttcgtca tgacgcgaaa gagcccgtaa ccgttgacga cgtagaaggg
      181 ggcgatcacg ccatacatcg cccccatcgg gcccggccac tccgtcccgg gcttgaaccc
      241 ggaaaagatg agcagcgcat tgaccggcat cgtcaaaacg ataatgacgg ccggcaccca
      301 gaccggccaa ccgcggccgg ccggcgcgcg attcgttagg cgaggcccgc ccggccagac
      361 gagatcgtcg atcaggagca ggcagagcgc gatcgtgaga agattaaaaa aggcgtaatt
      421 accggtgagg ccgatcacga tctgcaagaa gatgatcgcg ccgcaaccga gcagccgcag
      481 ccggcgcggc agccagacga gaaacgcgcc cgctatttcc accacgagga caaaggcggt
      541 cgagaaatgc ttgaaccact cgggtgattt gtccgcccac cagccgagta gcgtcggcag
      601 cggttgcgtc cagtaatgaa aatcgagcgc gctcaggtcc caccaggaag cgtcgccgct
      661 ggtgagcttc accacgccgg acatcagcat cagtttgaag agcaggaaat ggaggaggaa
      721 gagcgcgggc cgggagagcg gcgcgaacga gtcgcggcgc ggccaccagc gccagggcgc
      781 aagaaagatc gcgagaaaac ccgtctcgag cagcaggatg tcccattgaa aactaagaaa
      841 ggtttgcccc gcgatcgtga gcgagaggta gagcgcgaag agcagcgcga gacagacaat
      901 cggtgcgatc tccacgatca gcagcacgga aagcagcgcg ccggcgccgc acagcagatg
      961 caaaaatgca ttgctcggat tcagccagca gaacgtcggc agaagaaaga aggctcgact
     1021 accgagctgt ttgtgcgcgt cccccaggag ttgcgagatc ggcatgaccc cgtgcgcacc
     1081 aatcagcccg tccacctgca cccagagcga ccaaaacgcg atcaggtaaa tcatcccgag
     1141 catgcgcaga aaccagcggc gcgcgtggaa gtaggtcggg cgccggacat ctttgcccca
     1201 gaacaaggat gtgacgatgt gcgccagctc gcgatgtccc gcgatgaaag cgtaggccgc
     1261 cttggaaacg ggcgcgactc ccgggacatg ctcgtaactc cagctccaga attttttgcc
     1321 gccgccccgg cctaacgaac ggtaaaccgc cgccgcgccg ctcaagacgc ggccatccgg
     1381 ttcgatcaat ttgaccgcgc gttcgaactc ggcgcgcgga acctccggga agcgtgcggc
     1441 ggcctcctgc agcgactcgt aatccaccgc cccggccgtg atctcgcgcc agcgcgcgat
     1501 ccagagtttg cagaatccac aatcgccgtc gtaaaccatc agcattttcg agggcgggtt
     1561 ggcgactcga ttggcgggat tggggttcga tgtcaacaat gaacgttcaa cgttcaacga
     1621 tcaaggttca attgggaaac tggcgctgct gggagatgag cgctggacgt tgtccgcatc
     1681 acaccgcgaa ttccgcccaa accggcgcat gatcggacgg gtccttgcct ttgcgcatct
     1741 cgcgatcgac tcccgcggcg gtgcatttcg ctgccagctc gcggccggcc agcaccgcgt
     1801 cgatccgcag tccgcgattt ttcgggaagg ccagcatccg gtaatcccac caactgaaaa
     1861 tcccggcctc cggatgacgc aggcggagcg tatcggccag ccccaccgcg cagagcgcac
     1921 gaaaggccgc gcgctctttc tccgaacaca tgatagcgcc gcgccagagc gcgggatcgt
     1981 gcacatcggc gtcgttaggc gcgacgttat agtcgccgcc gaccacaatg cgctcaattg
     2041 cgccctcctt ttcccgcagg catttcgtga ggcgctcata ccaggcgagc ttgtagtcgt
     2101 aggccggcgc gccgatcgcc tgcccgttag gcgcataaat cgagacgacc cgcactccgc
     2161 cgacgctggc cgcgatcacg cgcgcctgtt catccgcgac ctcgtcgcag aatccgatcc
     2221 gcacctcgcg cggctcctcc tgcgcgagaa tcgcaacccc gttataggac ttctgcccgt
     2281 gaaagaccgc ggcgtagccc gcgtcgcgca gggccttctg cggaaactgc tcatcggtgc
     2341 acttcgtttc ctgcaggcag accacgtccg ggtttgtctc gcgcagccag agcaacaacc
     2401 ggtcctgtcg tttgcggagt gaattgatgt tccaggagac aatgcgcacg tcatctcttg
     2461 tagcgatggt ctctgaccgt cgcaaacggc gaggcaccag gagggacgac cagagcctgc
     2521 ccggcgcagc gggggtcgtc cgagcaacgc ggacgggaca gcgcccgtcg ctccaaaatc
     2581 acaactcgat ttcgacgccc ggcggcggca cgatgatttc gctccccggc agcgccaccg
     2641 cggcggaagc gcgcacccat tccaccgccg gcccatcgcc gtgcaccagc accactttct
     2701 tcggcgcgac cttcgtaatg taggcgagga tcgattcgcg ggaagcgtgg gcgctgaact
     2761 ggaattcctc gacgcggcag cggagcggtt gggccggctc ctcttcatcg agcgacagaa
     2821 actcgttagg cgcggtcctc cgcagaacgc ccgcgggcga ctctggatcg gcgtaaccga
     2881 caaaaaagat ggagtgctcg ggccgatcga tgatccggcg cgcgaaaata ttcgagagcg
     2941 ttttcggcgt catcatcccg ctggagagcg catagatacg gccgggccgc gccggggcgt
     3001 cggcgatcgt ttttccgttc aggacgaagg gcccgacctc gggaaacaat tgcaggcgcg
     3061 gcaggacacg gcgcgattga ttcgcccggc gatcgtaaat ctcggtcatc ttcgagctca
     3121 acccgccgat ataaatcggg acgtccgcga tcattttgtt gcgcttgaac ttgtaaaaca
     3181 tcgacagcac ctcctgcgtc ttgccgagcg cgaagaccgg cacgaggacg catccgccgc
     3241 gggcaaacgc gcgctcgatg gccgcggcaa aacgccgttc ttcggcgtcg cgcgaaaaac
     3301 ctttcgccaa cggcgaatcg ccacgtgtcg cctcgataat cagcacatcg atccccgact
     3361 cgggaaagac cgcgcccttg agcaacgtca ggtcgtcgaa gttcacatcg ccggtgtaga
     3421 agacggtccg gccttcggcc cgcaacagga taccggcggc accgaggacg tggcccgcgt
     3481 cataaaattc gaaactcagc tcgtcgttag gccgggccgg gctgcgttcc ccatccaccg
     3541 tgaacggctg gcccacggga caatgctgcc agcgatccgt cgcccgttcc gtttcgcgat
     3601 gagtaaagag cggataaatc acgcccacct cttcgcgctc ccgtgtcatc acattgactg
     3661 agttgtggag caaagcctcg ccgatcgcgg ccgtcggttc cgtcatgaaa atgcgcgcct
     3721 gcggctgttg ccgcatcagc accgggagcg acccgacgtg atcgagatgc gcgtgcgaga
     3781 ggagcagcgc atcaaccgtc cgcccggcca gcgtgccgag gagcggcagc gccatttcgc
     3841 cttcttcctt gggatgcatg ccgcaatcga gcacgacccc ctgcccgcct actccgagat
     3901 aataacagtt ggcgccgatc tccgtccggc gcgtcaggtt gatgaacttc aaaattgagc
     3961 gccaacttgc ccgttccgct tttccgagca accggcaaat ttaatccgct ggaaaacccg
     4021 cccgtggcgc aggctcgtca atcggtcttc cagtaggtgt tggtccccct cccacgaaag
     4081 aggatctgcg aggtcgtgga acacgcaaca ggcttcccgc gcaggtagcc cggcaagaaa
     4141 gtgaccttgt tgatccggtc catcaccgcc ttgccaaaac ccgacccgag cggggactca
     4201 taggtaattt ttgacgctaa cagttttccg gtagcatccg tgtcgagctt aacgcagacc
     4261 gtcccggaca gcatgtggtc cgacggatag gcaaatcctt cgaaattgtc gccccaccaa
     4321 aagacttcct gcggacagat gaagtcatca ccttttttga gatgctccgc ctcttgatta
     4381 agaaaaattc gcaaatgcgg ttttccatca atcacgacaa agatcatcgt gccagagacc
     4441 acggccgccg ctggttttcc gtgatagaca gccggaatga atctggcgta tcggcacttg
     4501 tcgatcgctt cttccgccag cgcatccgag ttgggtgtcc gataataggt ccacattcca
     4561 ttgctttctc caaacg
//
LOCUS       DATMNS010000045         9337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_413312, whole genome shotgun sequence.
ACCESSION   DATMNS010000045 DATMNS010000000
VERSION     DATMNS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9337
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413312"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VNW28_01740"
     CDS             <1..680
                     /locus_tag="VNW28_01740"
                     /inference="COORDINATES: protein motif:HMM:NF025099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbon starvation CstA 5TM domain-containing
                     protein"
                     /protein_id="HXA08672.1"
                     /translation="TALALWYHFAIMFEALFILTTLDAGTRVGRFLLQDLLGQFWRPL
                     GDTGSWSANIVTSALLVAAWGWFLYQGVLDPLGGINSLWPLFGLANQLLSVIALCLGT
                     TILIKMGKARYLWTTLGPLAFMIVVTFSAGYLKIWSADPRIGFLSGANMLDAKTAAAS
                     STVDFARQAFIWRIDALVALAFLVLVFLIVTGCARQWWRLLRGTKPIVLHESEYVPLA
                     RVRVAIR"
     gene            687..1682
                     /locus_tag="VNW28_01745"
     CDS             687..1682
                     /locus_tag="VNW28_01745"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08673.1"
                     /translation="MREKEMLAVVAALARQPTAPFREEAVRAEIEMQLRRCPYVDFER
                     DAFGNVIAHYRRGRRKAEWAFAAHMDHPGWVRGQNNEMVFLGSVPERFRRDPKTKSFG
                     DFAMWDLAPFEIHARQIHSRACDDLLGCAEIICLFRQLEELGAPAHCVGLFTRAEEVG
                     FVGAIKLAQSDILPRSLTIVSLETSTPRGTAEIGKGPIVRVGDKVSSFDGPATARLLS
                     AAQEEKIPVQRCLLDGGTCEATAYQLYGYTSAAASIALGNYHNCTPEGTIAPEYVAID
                     DFVGMVRLCMGLVLRGGTRSDPQKMLRKKLEKNAASYRVFYSSSKAPSKDSGARP"
     gene            complement(1753..2157)
                     /locus_tag="VNW28_01750"
     CDS             complement(1753..2157)
                     /locus_tag="VNW28_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08674.1"
                     /translation="MKHLLPLLCLTTLLLGTARAEDAVPAVELKQKSNFDAANARDPF
                     WPIGWKRPNPKNPNTNAGPDLSPDAFALTSVTTGTGGHFAILNGKIIQEGQQFGLQLG
                     AQVYQVTLRSIEDGQVVLVWQENEIVVPLRRK"
     gene            complement(2154..2717)
                     /locus_tag="VNW28_01755"
     CDS             complement(2154..2717)
                     /locus_tag="VNW28_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08675.1"
                     /translation="MKKFNKDQIQKIALSTIGFIALIYCYFNFFLGPLNKSRASMTQA
                     IAEVQEKTASSKTELKKTANLEMQAKQATVRYEALKHTTAEGAPIAWFPPKMRTFFNN
                     QGIDKSAARLESSGDFKQPELSNWIRDTWVIELPQSDYDVLGKAIAELENTEPLLNIQ
                     KIVIHAVPEEPQYQQVSLAVQTVLLKK"
     gene            complement(2720..3376)
                     /locus_tag="VNW28_01760"
     CDS             complement(2720..3376)
                     /locus_tag="VNW28_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08676.1"
                     /translation="MALQLNLLHEEITEQRQRQRDPLKLGVIALIAFGVILFLYYGWN
                     AYQTIQIKSRLNAANSEWQKVEPKVTAAQKRAAELHQIIDSTMVLNGLIDGRFYWAPF
                     LARVSHCVAPNIQVTSLDGGIVEADQSVAVALEGIAAAREPRAAAEDLRQLLIEQLEK
                     SYSAVKVNFKNLEDLDTVVNLSGVPTPSARFVLSVTFNPKPGDDSKAEEPPKDTTKKK
                     "
     gene            complement(3379..4452)
                     /gene="pilM"
                     /locus_tag="VNW28_01765"
     CDS             complement(3379..4452)
                     /gene="pilM"
                     /locus_tag="VNW28_01765"
                     /inference="COORDINATES: protein motif:HMM:NF022554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HXA08677.1"
                     /translation="MAKRANSVIGIDLGKHVFKSVLLQRKGDHRIVLTNYASRAVPDE
                     IKNADELAQQIKLLLKDLGGSAKSYAVAVSDPDALLRIIEQPNTPIDLLRSALRLNGL
                     AVLNQECKNFVLDCAPVSATSAVALAGVTPGGAPVEKVANSGKTRYLVGGMLRPMVKQ
                     ISEAFSKIKTPANVLQLAPICSFNAFEFAYPEIFNNEAFLLLDMGHQQSTVLIGQKTE
                     LMLVRSVDYGGKLFGEALIADGAVDSTTARVLLQQGDAGMADTCRNSLLGLATEVRNS
                     IGFFEGQCEESIHRIFVSGGMARTEMILQTLSDELGLPCEIWDPLETCEVALPSGKRQ
                     ALSSEFVSLNVACGAAISYLKAA"
     gene            complement(4536..5936)
                     /locus_tag="VNW28_01770"
     CDS             complement(4536..5936)
                     /locus_tag="VNW28_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXA08678.1"
                     /translation="MDVGTNKKAFQINLDAKRYGTFAEIGAGQEVARRFFQVGGASGT
                     IAKTMSAYDMQFSDAIYGATDRYVSRARLQTMLDREYRLLIERLDQKMGDERTFFVFA
                     DTVAARSFKQHNESHGWLGMRFQSKTRSEPSQIIIHVRMLDEGNVDQQEALGIIGVNL
                     IYGAFYCQQPEKLIASLHENLPSDRMQIDMIKFSGPAYEGVDNRLMSLQLVSQGLSDA
                     VMFTANGESVQPAEILHKKAIIVERGSFRPVTYATNDMLEGARKIFLNRPGAKEEDLV
                     VLMEMTLENLLSEGQLNHADFLARVDILGSLGRTVLISKFGEYYRLAGYLSRYTNQMV
                     ALVMGIPSLHEIFDEKYYLNLEGGILEALGRMFKNGLKLFVYPIEDEATGELITARQL
                     AVAPNLHHLYQYLIENRFIEGIADFHREYLKIFPPGVLAKMRAGDPTWESMVPPEVSR
                     MIKAREYFGYRRPVAA"
     gene            complement(6035..6796)
                     /locus_tag="VNW28_01775"
     CDS             complement(6035..6796)
                     /locus_tag="VNW28_01775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA08679.1"
                     /translation="MRAPILLLLTATALCLTPQVFGAPVAHAEADATFAKASADYAAG
                     HFPAAIAGYESLVKSHQWNSSLFYDLGNACFRAGDPGRAILNYERALALDPAQPEARA
                     NLQLVRDQARALELTGNWAEQHLSYLTRDQYAWLAATAFWGAVAIFCGLSFSRRRPAV
                     WIFALLLSGIISGGAAFAVYELETGSAGNDLAIVTGRNIQARLATTESAGAVLLLPPG
                     SEIKILSTRGDWLYAALPNELRGWIPTDSAERVRM"
     gene            complement(6798..8663)
                     /locus_tag="VNW28_01780"
     CDS             complement(6798..8663)
                     /locus_tag="VNW28_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HXA08680.1"
                     /translation="MAFHPSRIVRSVLALLLVGGAVQVVRADAPTVTAVLTSSETEVD
                     QPVQLQIKVTGDRSATPPSDINVDGLEIRYTGQSQLVEGRNFRFTYSFVYSYTILPLK
                     AGTFTIPSQVVQASGGRLRTPALTLNVSPNDDGTTTSRRRSGGGSSALDEKKIVFAEL
                     IIPKKTAYVGESVPAEIRLGIYTRIPHRLVEGANLSGQGFTAQRMPNPEQSTESINGR
                     PYDIVTFKTAITAVRSGKLEISAKDAKAIVQIPRRDGSRPRSPFDTFGMNDPFSDPFF
                     NDPFAGVGEQKEVTFSSETTTLEIKPLPPNAPPSFAGAVGLFSLATDVKPKTAQIGDP
                     LTVTATISGRGNFDRVTAPVLESDRGWHTYPPGSSFKPDDDIGLSGNKTFEMVLTPNE
                     AKKAVPPLIFSYFDPLKETYVTLKGDKLPVVVEGGGVPAATPAIASGSTTSPPATARS
                     AAAPQAQDILYQLNDHGSWDQNFTPIFMRPAFWTAQAVPFLGLIGFFGWEARRRRLEN
                     RAGQRLAAWEHETAELQRKLRRADDPPDQYFAEALRLVQLKTALASRGRGIEPNSVDA
                     ETAVAAFDLPDEKRDRVRELFRRSDELRYSGRPNGNATVAEQTRREVLELIDSLG"
     gene            complement(8703..>9337)
                     /locus_tag="VNW28_01785"
     CDS             complement(8703..>9337)
                     /locus_tag="VNW28_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08681.1"
                     /translation="GQGSSKDQKPENGKPSGQSPTPTPTPTPQPAGSPTPSPEPSPSS
                     SPSPSGSPTPTPGEDKEQKGPGESPTPTPSPTPSEGEQAGGSPTPGEEGTPSPTPGEE
                     GIPSPTPGEEGTPSPTPGPSGQNGGENGEQPSPTPAGTPGKAPTGEVRGAGEQKPNET
                     PAAQVAAVEPEKEGEMSPKQALALLESMKDEEQKVQFDEHKRVRPVYKDW"
BASE COUNT         1647 a         2717 c         2947 g         2026 t
ORIGIN      
        1 cgaccgcgct cgcgctctgg tatcacttcg cgatcatgtt cgaggcgctc tttattctca
       61 ccacactcga cgccggaacg cgggtggggc gttttctcct gcaggatttg ctgggccaat
      121 tctggcgccc gctcggcgac accgggtcgt ggagcgcgaa tattgtcacg agcgcgctgc
      181 ttgtcgcagc gtggggctgg ttcctctacc agggtgtgct cgatccgtta ggcggaatca
      241 acagtctctg gccgctcttc ggcctggcca atcaacttct ctcggtcatc gcgctttgcc
      301 tcgggacaac gatcctgatc aagatgggca aagcgcgcta cctctggacc acgctcgggc
      361 cgctcgcctt catgatcgtg gtcacgtttt ccgccggtta tctcaagatc tggtcggcgg
      421 atccgagaat cgggttcctc agcggagcca acatgctcga cgcgaagacg gcggctgcgt
      481 cgagcaccgt cgattttgcg cggcaggctt tcatctggcg gatcgatgcg ctggtggcgc
      541 tcgctttcct ggtgcttgtt ttcctgattg tgaccgggtg cgcccggcaa tggtggcggc
      601 tcctgcgcgg gacgaaaccg atcgtgctcc acgaaagcga gtatgttcct ctggcccggg
      661 tgcgcgtggc gattcgttag tccgggatgc gcgaaaaaga aatgctcgcc gtcgtggccg
      721 cgttggccag gcagccgacc gcgccttttc gcgaggaggc ggtgcgggcg gagattgaga
      781 tgcagctgcg gcgatgtcca tatgtcgact ttgagcgcga tgcttttggg aacgtgatcg
      841 cgcattatcg gcgggggcgc cgcaaggcgg aatgggcgtt cgccgcgcac atggatcacc
      901 cgggctgggt gcgcggccag aacaacgaga tggtattcct cggctccgtg ccggagcgct
      961 tccggcgcga tccgaaaacg aagtcgttcg gcgattttgc gatgtgggat ctcgcgcctt
     1021 tcgagattca cgcgcggcag attcattcgc gcgcttgtga tgatctgctc gggtgcgcgg
     1081 aaatcatctg cctctttcgt caattggaag aactgggtgc gcccgcgcat tgcgttggcc
     1141 tttttacccg ggcggaagaa gttggatttg tcggcgcgat caaacttgcg cagtcggata
     1201 tcctgccgcg cagcctaacg attgtctcgc tcgaaacaag cacaccacgt ggcacggcgg
     1261 aaattgggaa aggtccaatc gtgcgggtgg gggacaaggt ttccagcttt gacggcccgg
     1321 ccacggcgcg actcttgagc gccgcgcagg aggagaaaat cccggtgcag cgttgcctgc
     1381 tcgatggcgg gacatgcgaa gcgacggcgt atcagcttta cggttatacc tccgccgcgg
     1441 cgagcattgc gcttggcaac tatcataact gcacgccgga gggaacgatt gcgccggagt
     1501 atgtcgcaat tgacgatttt gtcgggatgg tgcgactctg tatggggctg gttttgcgcg
     1561 gagggacaag gagcgatccg cagaaaatgc tccggaagaa gctggagaag aacgccgcga
     1621 gttatcgcgt tttctattcg tcatcaaaag cgccgtcgaa ggattccggt gcgcgaccgt
     1681 aaaatttccg ccgcggaatc gctcgactcc gctgcgtttc gctcgggatg acgggcttcg
     1741 cggccggtcg gactacttgc ggcgcagggg caccacaatc tcgttctcct gccagaccag
     1801 aacgacctga ccgtcctcga tcgagcgcag ggtgacctgg tagacctggg cgccgagctg
     1861 aaggccgaac tgctggcctt cctggatgat tttgccattc aggatggcaa agtgcccgcc
     1921 ggtaccggtc gtgaccgagg tgagggcaaa cgcatccggc gagaggtctg ggccggcgtt
     1981 ggtatttgga ttcttcgggt ttggtctttt ccaaccgatc ggccagaaag gatcgcgggc
     2041 gttggccgcg tcgaagttgg atttttgctt tagctctact gccgggacgg cgtcctcggc
     2101 gcgggccgta cccagtaaca aagtcgttag gcaaaggaga ggcaggagat gtttcatttc
     2161 ttcaagagca ctgtttgcac cgcaaggctg acctgttggt actgcggttc ctctggcacg
     2221 gcgtggatga cgattttttg gatgttgagg agcggctcgg tgttttccag ctcggcaatg
     2281 gctttcccga ggacgtcgta gtcgctctgg ggcaactcga tgacccaggt gtccctgatc
     2341 caattagaga gctccggctg cttgaaatct ccgctcgatt ccaggcgcgc ggcggatttg
     2401 tcgattccct ggttgttaaa gaaggtgcgc atcttcggcg ggaaccacgc aatcggcgcg
     2461 ccttcggcgg tggtgtgctt gagcgcttca tagcggaccg tcgcctgctt ggcttgcatt
     2521 tcgaggttgg cggttttttt cagttcggtc ttggaggatg cggttttttc ctgcacctcg
     2581 gcgatcgcct gggtcatcga cgcgcggctc ttgttcagcg gcccgaggaa gaaattgaag
     2641 tagcaataaa tcaacgcgat gaaaccgatc gtgctcagag cgatcttttg aatctgatct
     2701 ttgttgaatt tcttcacctt catttctttt tcgtggtgtc cttcggtggt tcctccgctt
     2761 tgctgtcgtc gccgggcttg gggttgaagg tgacgctcag gacaaagcgg gcgctgggcg
     2821 tgggaacgcc gctgaggttt acgacggtgt cgaggtcctc gaggtttttg aagtttacct
     2881 ttacggcgct gtaacttttc tcgagttgct cgatcaggag ctgcctgaga tcttccgcgg
     2941 ccgcccgcgg ctcacgagcg gcagcgatgc cttcgagcgc gactgcgact gactggtctg
     3001 cttcgacgat cccgccgtcg agactggtta cctgaatgtt gggcgcgacg cagtgcgaga
     3061 cccgggccag aaatggcgcc caatagaagc gcccgtcgat cagaccgttg agtaccatgg
     3121 tgctgtcgat gatttggtga agttccgcag cccgcttctg agcggcggtg actttcggct
     3181 cgaccttttg ccactcgctg ttggcggcat tgagccggct cttgatctgg atcgtctggt
     3241 aggcgttcca gccgtagtag aggaagagga tcacgccaaa tgcgatcagg gcgatgacgc
     3301 cgagtttgag cgggtcgcgc tggcgctggc gctgttccgt gatttcctcg tgaagaagat
     3361 tgagctgcaa ggccatcgtt aagcggcttt cagataggag atggctgcgc cgcaggcgac
     3421 attcaaggag acgaattcgc tggagagtgc ctggcgcttg cccgatggca gcgccacctc
     3481 gcacgtttcc aacggatccc aaatttcgca gggcagtccc agttcatcac tcagggtctg
     3541 caaaatcatt tccgtcctcg ccatgccgcc ggaaacgaaa atccggtgga tgctttcctc
     3601 gcattgacct tcgaaaaatc cgatcgaatt gcgcacttcc gtagcgagac cgaggagcga
     3661 attgcggcag gtgtcggcca tgcccgcgtc gccttgctgc agcagcacgc gggcagtcgt
     3721 gctatccacc gcgccgtccg caatcaacgc ttcgccgaag agtttgccgc cgtagtcgac
     3781 gctccgcacg agcatgagct cggtcttctg gccgatcagc accgtgcttt gctggtggcc
     3841 catgtcgagg agcaggaagg cttcgttgtt gaagatctcc gggtaggcaa actcaaaagc
     3901 gttgaaggag cagatgggcg cgagctgcag cacgttggcg ggcgtcttta tcttgctgaa
     3961 cgcttcggaa atctgtttga ccatcgggcg cagcatcccg ccgacgagat agcgggtctt
     4021 gcccgagttc gcgacctttt cgaccggcgc gccgccgggt gtcacgccgg cgagcgcgac
     4081 cgccgaggtt gccgagacgg gcgcgcaatc cagcacgaaa tttttgcatt cctggttgag
     4141 gaccgcgagg ccgttgagac gaagggcgct gcggagcaga tcgatcggcg tgttcggctg
     4201 ttcgatgatg cgcaggagcg cgtcgggatc ggaaaccgcg acggcatagc tcttcgcgct
     4261 gccacccaga tctttgagga gcagtttgat ctgttgcgcc agttcgtcgg catttttgat
     4321 ctcatccggc accgcgcgag acgcgtaatt cgtcaggaca atgcggtgat ctccttttcg
     4381 ttgcaggagc acacttttga agacgtgttt gccgaggtcg atgccgatga ctgaattcgc
     4441 gcgtttggcc atggtcgttg cgcttgttat agcgaaaacc atgcctcgcc cgcaggcttt
     4501 atgaaaattc ccgcgctcta tggcgccggc tgatctcagg cggccaccgg gcgccgataa
     4561 ccaaaatatt cgcgggcctt gatcatccgc gaaacctccg gaggcaccat gctctcccag
     4621 gtgggatcac ccgcccgcat tttcgcgagc acgccgggcg gaaaaatctt caggtactca
     4681 cgatgaaaat ctgcaattcc ctcaatgaag cgattctcga tcaggtactg gtagagatga
     4741 tgcagattcg gcgcgaccgc gagctgccgc gcggtaatta attcgccggt cgcctcgtct
     4801 tcgatcggat agacgaatag cttcaggccg tttttgaaca tccgcccgag cgcttccaga
     4861 atcccgccct cgaggttgag gtaatatttc tcgtcgaaga tttcatgcag gctgggtatt
     4921 cccatgacaa gtgccaccat ctggttggtg tagcgggaaa gataaccggc gaggcggtag
     4981 tattcgccga acttcgaaat caaaaccgtt cggcctaacg aaccgagaat atcgacccgc
     5041 gcaaggaaat cggcgtggtt gagctgacct tcggagagca gattctccag cgtcatttcc
     5101 atcagcacaa ccaggtcctc ttccttcgct ccgggccggt tgaggaaaat ttttcgcgcg
     5161 ccctccagca tgtcgttggt cgcgtaggtc accggccgaa agctgccgcg ctccacgatg
     5221 atcgccttct tgtgcagaat ctccgccggc tggaccgatt cgccgttggc ggtgaacatg
     5281 accgcgtcgc tcaggccctg gcttacgagt tggaggctca tcagccggtt gtcgactccc
     5341 tcgtaagcgg gtccggaaaa tttgatcata tcgatctgca ttcggtccga cggcagattt
     5401 tcgtggagcg aggcgattaa tttctccggc tgctggcagt aaaacgcgcc atagatcaag
     5461 ttcacgccga tgatcccgag tgcctcctgc tgatcgacgt tcccttcatc gagcatccgc
     5521 acgtggatga tgatctggct cggctcgctg cgcgttttgg attggaaacg catcccgagc
     5581 cagccgtgcg actcgttatg ctgcttgaaa ctgcgggccg cgaccgtgtc cgcaaaaacg
     5641 aaaaaggtgc gctcgtcgcc catcttttga tcgaggcgtt cgatcaacaa tcggtattcg
     5701 cggtcgagca tcgtctggag ccgggcgcgg ctgacatagc gatccgtcgc gccgtagatc
     5761 gcgtcactga attgcatgtc ataggccgac atcgtcttcg cgatggtgcc ggaggcgccg
     5821 ccgacctgga aaaaacggcg cgccacttcc tgcccggcgc cgatttccgc gaacgtgccg
     5881 tagcgtttcg cgtcgagatt gatctggaac gctttcttgt tcgttccgac atccatttcg
     5941 ctcacggatc taagatgagg gcaatcggga gcttctcaac cgatggaaac acggcgcgct
     6001 aacagaccgg ggcggaaagg cccctcccac attactacat ccgcacccgc tcggcgctgt
     6061 cggtcgggat ccagccccgc aattcgttag gcaaggcggc gtagagccag tcgccccggg
     6121 tgctgagaat tttaatttcg ctccccggtg gcaggagcaa gaccgcgccc gcgctttccg
     6181 ttgtcgccag acgggcttgg atattcctgc cggtcacgat cgcgaggtca ttccccgcgc
     6241 ttcccgtctc gagctcataa accgcgaacg ccgcgccccc tgagatgatg cctgacaaca
     6301 agagcgcaaa aatccaaacc gccggccgtc gccgggaaaa agaaaggccg cagaaaattg
     6361 ccactgcgcc ccaaaaagcc gtggccgcga gccaggcgta ctgatcccgc gtcagataag
     6421 agaggtgctg ctccgcccag ttgcctgtca gttcgagggc ccgcgcctga tcgcgcacca
     6481 gttgcagatt ggcgcgtgct tcgggttgcg ccgggtcgag cgcgagggcg cgttcgtaat
     6541 tcagaatggc gcgcccggga tcaccggcgc ggaagcaggc gttgcccaga tcgtaaaaca
     6601 aacttgagtt ccactggtgg ctcttgacca gcgactcgta gcccgcgatc gccgcgggaa
     6661 agtggcccgc ggcgtaatcc gcgctggcct tggcgaaggt cgcatcggcc tcggcatggg
     6721 cgaccggcgc cccgaagacc tgcggggtca ggcaaagcgc cgtggcagtg agcagaagga
     6781 gaatgggcgc gcgcatttca gcccagactg tcgattaatt ccagcacctc gcgccgggtt
     6841 tgctcggcga cggtggcgtt gccgttgggc cggccactgt agcgcagctc gtcgctccgg
     6901 cggaatagct cgcgcacgcg atcccgtttt tcatccggca ggtcgaacgc tgccactgcc
     6961 gtctcggcgt ccaccgagtt cggctcgatt ccgcggccgc gcgacgcgag cgcggtcttg
     7021 agctgcacaa ggcgcagtgc ttcggcgaag tattggtcgg gcggatcatc cgcgcgccgc
     7081 agcttgcgct gcagctccgc ggtttcgtgt tcccaggccg cgagcctttg ccctgcgcga
     7141 ttctcaaggc ggcggcgccg cgcctcccag ccaaaaaaac cgatcaaccc gaggaacggg
     7201 accgcttgcg ctgtccaaaa agccggacgc atgaaaattg gagtgaagtt ttgatcccac
     7261 gaaccgtgat cgttcagctg atagaggatg tcctgcgcct gcggggccgc cgccgagcgc
     7321 gcggtcgccg gaggcgaggt ggtggagcca ctcgcgatcg ctggggtcgc ggcggggacg
     7381 ccaccgccct cgaccactac gggcagcttg tcgcctttga gtgtgacata ggtttccttc
     7441 aacggatcga agtaggaaaa gatcagcggc ggaacggctt ttttcgcctc gttaggcgta
     7501 agcaccattt cgaaagtctt gttgccgctc agcccgatgt cgtcgtctgg tttgaagctg
     7561 gagcccggcg gataagtgtg ccaaccgcga tcactctcca gaacgggcgc cgtgacccgg
     7621 tcgaaattgc cgcgcccgga aatggtcgcg gtcacggtga gcggatcgcc gatctgcgcg
     7681 gttttcggtt tcacgtccgt cgcgaggctg aataatccga ccgcgcccgc gaagctcggg
     7741 ggcgcattgg gcggcagcgg cttaatttca agcgtggtgg tttcactcga aaatgtcact
     7801 tccttctgct ccccgacacc ggcgaaagga tcgttaaaga atgggtcgga gaacggatca
     7861 ttcattccga aagtatcgaa gggcgaccgc gggcgactgc cgtcgcgccg cggaatctgg
     7921 acgatggcct tggcgtcctt ggcggagatt tccagcttac cgctgcgcac ggcggtgatc
     7981 gcggtcttga aggtgacaat gtcgtagggg cgtccattaa tcgactcggt ggactgctcg
     8041 ggattcggca tgcgctgggc ggtgaatccc tgtccgctca ggtttgctcc ttcgaccagg
     8101 cggtgcggga tgcgggtgta gatcccgagc cgaatctcgg ccggaacgct ttccccgacg
     8161 taagccgttt tcttcgggat gatcagctcg gcgaaaacaa ttttcttttc gtcgagcgca
     8221 ctgctgccac ccccgctgcg gcgtcgcgag gtggtcgtac cgtcgtcgtt aggcgaaacg
     8281 ttcagggtca gcgcgggagt gcgcagtcgg ccgccgctgg cctgcaccac ctgggacggg
     8341 atggtgaagg ttccggcttt cagcgggagg atcgtgtagc tgtaaacgaa gctgtaggtg
     8401 aaccgaaaat tccgtccttc gacgagctgt gactggccag tgtagcggat ttcgagacca
     8461 tccacattaa tgtcgctcgg cggagtcgcg ctccgatcac cggtcacttt gatttgcaac
     8521 tgcaccggct ggtcaacttc cgtttccgag ctggtcagga cggcggtcac ggtcggcgca
     8581 tcggcgcgca cgacctggac cgcgccgccc acgagcaaca gggcgagcac cgacctaacg
     8641 atgcgtgatg ggtgaaacgc cattttcaaa tcgaacatcg cacatcaaaa ccaaacatcc
     8701 cctcaccagt ccttatagac aggacggacg cgcttgtgtt catcgaactg cactttctgc
     8761 tcctcatctt tcatcgattc aaggagggcc aaggcctgtt tcggactcat ctcgccctct
     8821 ttctccggct caactgcggc gacctgtgcg gctggagtct cattcggttt ctgttcgccc
     8881 gcacctctaa cctccccggt cggcgctttg cccggtgtgc cggccggcgt cgggctgggt
     8941 tgctcgccgt tttcacctcc gttctggccg ctgggtcccg gggtcggcga aggcgttcct
     9001 tcctccccgg gtgtggggga gggaattcct tcttcgccag gtgtgggcga gggagttcct
     9061 tcttctccgg gagtcggcga accgcccgct tgctcacctt ctgaaggcgt gggactgggc
     9121 gtcggcgtgg gggattctcc cgggcctttc tgttccttat cctcgcccgg tgtcggggtc
     9181 gggcttccgc tcgggctggg gcttgaactc ggactcggct ccggagacgg ggtcggtgaa
     9241 cccgccggtt gaggtgtggg tgtcggcgtc ggcgtcgggg actgaccgga cggcttccca
     9301 ttttccggct tctgatcttt gctggagccc tggccat
//
LOCUS       DATMNS010000046         6391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3965803, whole genome shotgun sequence.
ACCESSION   DATMNS010000046 DATMNS010000000
VERSION     DATMNS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6391
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3965803"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1368)
                     /locus_tag="VNW28_01790"
     CDS             complement(<1..1368)
                     /locus_tag="VNW28_01790"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HXA08682.1"
                     /translation="MKIKLGRVTLLFALAVLITQILLAQEVSTDDLARRTVERRAVEA
                     VIWSMPAVNFDLMYQAMIRDAKAGAGSNKIVYWSKLSDWKNQTLTPNPDAVYFMPFFD
                     TKEVGPVVLEIPPADEGGSITGSVMDCWQAALEDVGPAGVDKGKGGKCLILPPDYKEK
                     IPDGYIAMPSGNFQGYALLRSILQSGGDTSVAKAVAYAKRMKLYPLSKAASPPATTFV
                     DASSVVFEANIPYDSRFFDSINRIVQEEPWLTRDKAMIDSIRTVGIEKGKAFSPDAKT
                     RAILDAAAKEAHAWLEAKYEALFTPPYYEGTGWALPMTPELINGLQTQFSDPNSYPVD
                     ARGVIYTMAFFSPKRSGAGSFYLMTIKDKSGYAFDGGNAYRLHVPPNAPVKQFWSATA
                     YDRATHGLIRNLPRASRSSQNPELQKNADGSVDVYFGPKAQAGRESNWIPTDRTGRFE
                     VLFRFY"
     gene            complement(1411..3009)
                     /locus_tag="VNW28_01795"
     CDS             complement(1411..3009)
                     /locus_tag="VNW28_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HXA08683.1"
                     /translation="MKTKRLLVLFTLLFAEISNSAQAQEKKPNILVIMGDDIGQTNVS
                     AYSMGVMGYRTPNIDRIAKEGMIFTDYYAEQSCTAGRSSFITGQCTLRTGLSKVGIPA
                     SPIGLQDRDVTIAQLLKTKGYATGQFGKNHLGDLNKYLPTVHGFDEFFGNLYHLNAEE
                     EPERRTYPQDPKFAEMFGPRGVLRCKATDVDDPTEQPRWGRIGKQTIEDSGPLTRKRM
                     ETIDDETSDAAVDYMQRQAKANKPFFCWMNTTRMHAFTHVREEHRDKPGLTSRTEYND
                     GMIEHDAVVGKILKALDDAGITDNTIVLYTTDNGPHKNSWPDGGTSPFRNEKATNWEG
                     AFRVPCMIRWPGHIKSGSVANEIVSGHDWFPTFLAAAGDPDVKDKLLKGMNVGGKTYK
                     VHLDGYNQLPYLTGQVDKSPRRGFFYFNDDGDIVALRVENWKIVFLEQRAPGTLAVWG
                     EPFTPLRLPKMYDLHADPYEQADITSNSYYAWELEQGYILLAGSTLTGEFLKTFKDFP
                     PSQRPATFTIDQAMEKMKQNLGSQ"
     gene            complement(3053..3328)
                     /locus_tag="VNW28_01800"
     CDS             complement(3053..3328)
                     /locus_tag="VNW28_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXA08684.1"
                     /translation="MAWIPGGEFSMGAAISGHGSCEMAMQSNDSEPIHWRHLQGPESD
                     IRGRDNYPVVQVAYPDAEAYAKWAGKRLPTEAEFEAIPAPITSAFAV"
     gene            complement(3983..4056)
                     /locus_tag="VNW28_01805"
     tRNA            complement(3983..4056)
                     /locus_tag="VNW28_01805"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4020..4022),aa:Asp,seq:gtc)
     gene            4195..5886
                     /locus_tag="VNW28_01810"
     CDS             4195..5886
                     /locus_tag="VNW28_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXA08685.1"
                     /translation="MSDESKQNPEDNGSPDDEREISLLEAFDRAVALLPANELARDAL
                     LALRAEIEAQEEMVGDAREMIEKLEQVIKKVTSPANRIGTFLGSPARDTAQIVVGGAD
                     YYCNVDPRISLARLQKGTRVLVNEAYVIVGDLGFETAGPVTKIAEVLGPDRLRVGAEH
                     GTQSFVLQRSAQLAKAPLKAGDDVRVDANFRVALELLASPQSRDHYLDEVPELPWEKV
                     GGQQAALQAIRDAIELPLLHEELFQKFQHATPKGFLLYGPPGCGKTLIGKATAYNLTK
                     QLRGKSGADMREYFMHIKGPEILNMWVGESERMVREIFATAREKRREGYMPFLFIDEA
                     ESILGTRRASRYSSILSTLVPMFCTEMDGIDSLNEVVIILASNRADLIDPAILRPGRI
                     DRKIKVSRPNKEGAREIYRIYLTDDLPYDAALLAGTESKAAVIDALIGRLLEAQFARK
                     EENRLLEVTLRSGRKEALFRSDVISGAIIASVVERAKGMAIKRSIASGQDEGISEADL
                     QIAFNAEYLENDLLPATDITEDWLKLIDYDPDNVVKISPVRLVDRAKSKTTSGVI"
     gene            5961..>6391
                     /locus_tag="VNW28_01815"
     CDS             5961..>6391
                     /locus_tag="VNW28_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08686.1"
                     /translation="MKTLPFVLAAFAAATVLAQAQETIQQPDQAKLEAMTARFAPTEI
                     TADVSQLSPNDRKVLAKLVEASQIIDGIFLRQSWAGNPSMLLDLAGRDSAEDRARLHY
                     FILNRGPWSNLEHNAPFVLGAPQKPAGAGFYPPNATKVEIEA"
BASE COUNT         1263 a         1907 c         1851 g         1370 t
ORIGIN      
        1 atagaaacga aacagaacct cgaaccttcc cgtcctgtcc gttggaatcc agttcgattc
       61 cctgccggcc tgagccttgg gcccgaaata gacgtctacc gagccgtcgg cgtttttttg
      121 cagctcgggg ttctgcgaag accggcttgc gcgcggcaga tttcgaatga gcccgtgcgt
      181 cgcgcgatca taagccgtgg ccgaccagaa ttgttttacc ggcgcgtttg gagggacgtg
      241 caaacgatat gcgttgccgc cgtcgaaggc gtagccggat ttgtccttga tcgtcattaa
      301 gtaaaacgat ccggcgcccg accgtttcgg gctgaaaaaa gccatcgtat aaataacgcc
      361 acgagcatcg accggataac tgttcggatc ggagaattgg gtctgaagac cgtttatcaa
      421 ctcgggcgtc atcggaagcg cccaaccggt gccctcgtag tacggtggtg tgaacagcgc
      481 ttcgtatttt gcttccagcc aggcgtgcgc ctctttcgcc gcggcgtcga ggatggcacg
      541 tgttttcgca tccgggctaa acgccttccc tttttcgatg cccaccgttc tgatagaatc
      601 gatcatcgct ttgtcgcgtg tcagccaagg ctcttcttgc acgatgcggt taatcgaatc
      661 gaagaaccgc gagtcgtacg ggatgttcgc ctcgaagacg acactggacg cgtcgacgaa
      721 tgttgttgcc ggggggctcg ccgctttgga gagcggatac aatttcatcc gcttcgcgta
      781 ggcgaccgcc tttgcgacac ttgtatcacc gccgctttgc aaaattgatc gcagcaacgc
      841 gtagccttgg aaatttcccg aaggcatcgc gatgtaaccg tcggggattt tttccttata
      901 gtcaggcggt aggatcagac acttgccacc tttgcccttg tccaccccgg ccgggccaac
      961 gtcctccaac gccgcctgcc aacaatccat tacggatcca gtgatcgatc cgccttcgtc
     1021 ggccggcgga atctcgagca caaccgggcc gacctccttt gtatcgaaga acggcatgaa
     1081 gtagacggcg tccgggttgg gcgtcagggt ctggttcttc cagtccgata gttttgacca
     1141 atagacgatc ttattgctgc ctgcgcccgc cttcgcgtcg cggatcatcg cctgatacat
     1201 cagatcgaag ttcaccgccg gcatgctcca tatgacggct tcgaccgcgc gacgttccac
     1261 tgttcgccgg gccaggtcgt cggttgaaac ctcttgtgct aacaagatct gcgtgataag
     1321 cacggctagc gcaaaaagta acgtaactct tccgagctta attttcatga agccgccctt
     1381 tattgtcatt actcgttagg caaagcggat tcactgactg ccgaggttct gcttcatctt
     1441 ttccatcgct tggtcgatgg tgaatgttgc tgggcgttga cttggaggaa aatctttgaa
     1501 cgtcttgagg aactcgccgg ttagcgtgct gcccgccaat aggatgtagc cctgttccag
     1561 ttcccaggcg tagtaggaat tggacgtgat gtcggcctgc tcatagggat cggcgtgcag
     1621 gtcgtacatc ttcggaaggc gcagcggcgt gaacggctcg ccccagactg cgagcgtgcc
     1681 gggtgcgcgc tgttccagga agacgatctt ccagttctcc acccgaagag cgactatgtc
     1741 gccgtcgtcg ttgaaataga agaacccgcg ccgcgggctt ttgtcgacct ggccggtgag
     1801 atacggaagc tggttgtagc cgtcgaggtg caccttgtag gtcttgccgc cgacgttcat
     1861 gcctttaaga agtttgtcct tcacatcggg atcaccagcc gccgcgagga aggttgggaa
     1921 ccagtcgtgg ccgctaacaa tctcgttggc gaccgagccg gatttgatgt ggcccggcca
     1981 gcggatcatg cacggcacgc ggaaggcccc ttcccaattc gtcgctttct catttcggaa
     2041 aggcgaggtg ccgccatccg gccaactgtt cttgtgcggg ccgttatcgg tggtgtagag
     2101 gacgatcgta ttgtcggtga tgcccgcgtc gtcgagggct ttcaggatct tcccgacgac
     2161 ggcgtcgtgc tcgatcatgc cgtcgttgta ttccgttcgc gaagtgagac cgggcttatc
     2221 gcgatgttcc tcccgcacgt gcgtgaaagc gtgcatgcgc gtcgtgttca tccagcaaaa
     2281 gaaaggcttg ttcgccttgg cctggcgctg catgtaatcc accgcagcat cggaggtctc
     2341 atcatcgatc gtctccatcc gcttcctggt cagcgggccg gagtcttcga tagtctgctt
     2401 gccaattcgg ccccagcgcg gctgctcggt cggatcatcg acgtcggtag ctttgcatcg
     2461 cagcacgccg cggggaccaa acatctccgc gaacttcgga tcctgcggat aggttcgtcg
     2521 ctcgggttct tcttcggcat tgaggtgata aaggttgccg aagaactcgt cgaagccgtg
     2581 gactgtcggg aggtacttat tcaagtctcc taaatgattt tttccgaact ggccggtagc
     2641 gtaacctttg gtcttcagga gctgggcgat cgtcacgtcg cggtcctgaa gcccgatagg
     2701 cgaggcgggt atgccgactt ttgaaagtcc agtccgcagc gtgcattggc cagtgatgaa
     2761 cgacgaccgc ccggccgtac aactttgctc cgcgtagtaa tcggtgaaaa tcattccctc
     2821 cttcgcgatg cgatcgatgt taggcgtgcg gtagcccatc acgcccatcg aataggcgct
     2881 gacattggtc tgcccaatgt cgtcgcccat gatgacgaga atgtttggtt tcttctcctg
     2941 agcttgcgcg gagttcgata tttctgcgaa caagagagtg aatagaacga gcagtcgttt
     3001 tgttttcata ggctggtctt ttatttggtt gcgacttgtt gcggagttga tttcacaccg
     3061 cgaaagccga ggtgattggt gccggtatgg cttcgaactc ggcttcggtt ggtaaacgtt
     3121 tgcccgccca cttcgcgtac gcctcagcat cagggtaggc gacttgaact accgggtagt
     3181 tgtctctacc tcttatatcg ctttctggcc cttgcaggtg acgccagtga atcggctccg
     3241 agtcgttcga ctgcatcgcc atttcgcaac tgccgtgtcc gctaatggca gcgcccattg
     3301 agaattcacc acccggaatc caagccatcc cggcggattt cctttcggcc gggcggctgc
     3361 gttgacgatt gtcgctctag gctcggttcg ctgccccgct gtcgcggcga gatcttctgt
     3421 gattgcggac tgcgcggagg cgaacaggag gagagtcgcg agtacccggt taagggccgc
     3481 gatcagcttc ccctcactga attgagcgtt catgcaaaca ctccgcccgg cactggctcc
     3541 gagccaatgc cctccgcccc tcctgtttgc caccccgcag ggagtctgac aatctccctt
     3601 tttgagaatt tttcgaagcg attcaagtca aatttcgcga tgatgcggcc ggggcggcaa
     3661 gactgtaccc gttcttccaa aaagcgggcc tagattccca aatgtctggc cgcccgataa
     3721 taatcggcgc cgtcgtcgcc catttcgtgg ccattccgct tcccgtttcg gaaaatcggc
     3781 cgcttctctg cgagtgcgca gatcatcatc gcccgagcag cttaatgaaa ttcgttgtct
     3841 tagtcttgag gtaaccagca ggttggcacc ttgtcagacc aacaaatgat ggcagcgcga
     3901 atctggttta atgccagttg ctaattcgcc aggcccctcc cggaccttcg gcacatccgg
     3961 agttaacgca atacggaaac cccggagcct acggggctcg aacccgcaac ctctgccgtg
     4021 acagggcagc gctctaacca attgagctaa ggctcctcga aaagagaggc caccgtaggg
     4081 cgcggccccg cctccttcaa ccgatttttg ccatcttcgc aggcacgaag tttgcggcct
     4141 gaatcgggca cgcgtattat tgaccgtaaa cgtcagcccg agggacgaca gggaatgagc
     4201 gacgaatcca agcaaaaccc ggaagacaac ggttcgccgg acgacgagcg cgagatcagt
     4261 ctgctggaag cgttcgatcg cgcggtcgcg cttctcccgg cgaacgagct ggcccgcgac
     4321 gcgctcctcg cgctccgcgc tgaaatcgaa gcgcaggagg aaatggtcgg cgatgcgcgc
     4381 gagatgatcg agaagctcga gcaggtgatc aagaaggtga cctcgcctgc caatcgcatc
     4441 ggcactttcc tcggcagccc ggcgcgcgac accgcgcaaa tcgtggtcgg gggcgcggat
     4501 tattattgca acgtcgatcc gcggatcagc ctcgcccggc tgcaaaaggg cacgcgcgtc
     4561 ctggtcaacg aagcctacgt catcgtcggc gacctcggtt tcgaaactgc cgggccagta
     4621 accaagatcg ccgaggtcct gggcccggat cggctgcgcg tcggcgcgga gcatggaacg
     4681 cagtcgttcg tcctgcagcg ctccgcccag ctcgcgaaag cgccgctcaa agcgggcgac
     4741 gacgtgcggg tggatgcgaa ttttcgcgtc gcgctcgagc tgctggccag cccgcaatcg
     4801 cgtgaccatt atctggacga agtgccggag ctgccgtggg aaaaagtcgg cggccagcag
     4861 gcggcgctgc aggcgatccg cgatgcgatc gagctgccgc tcctgcacga ggagttgttt
     4921 caaaaattcc aacacgccac gccgaaaggc ttccttcttt acggcccgcc cgggtgcggc
     4981 aagacgctca tcggcaaagc gaccgcctat aacctgacga aacagctccg cggcaaatcg
     5041 ggcgccgata tgcgggaata tttcatgcac atcaaaggcc ccgagatcct caacatgtgg
     5101 gttggcgaat cggagcggat ggtgcgcgaa atcttcgcca ccgcccgcga gaaacggcgc
     5161 gaaggctata tgccgtttct cttcatcgac gaagcggaaa gtattctcgg cacgcgtcgc
     5221 gcgtcgcgtt actcgagcat tctctccact ctcgtcccga tgttttgcac ggaaatggac
     5281 ggaatcgatt cgctgaacga ggtcgtcatc atcctcgcct cgaaccgcgc cgatttgatc
     5341 gatcccgcga tcctgcgccc cggccggatt gatcgcaaaa tcaaggtgag ccggccgaac
     5401 aaggaaggcg cgcgcgaaat ttatcggatc tatctgaccg acgacctccc ttacgacgcc
     5461 gcgcttctcg ccgggacgga atcgaaagcc gccgtgatcg acgcgctcat cggccggctg
     5521 ctcgaggcgc aatttgcgag gaaggaagaa aaccgcctgc tcgaagtgac cctgcgtagc
     5581 ggacgcaagg aggcgctttt ccgcagcgac gtgatcagcg gcgcgatcat cgcctcagtc
     5641 gtcgaacgag ccaaggggat ggcgatcaag cgctcgatcg cgagcggcca agacgaaggc
     5701 atcagcgaag ccgatttgca gatcgcgttc aacgccgagt atctggaaaa cgaccttctg
     5761 cccgcgaccg acatcacgga agattggctc aagttgatcg actacgatcc ggacaacgtg
     5821 gtcaagattt cgccggtccg tctggtcgat cgcgcgaagt cgaagacgac atcgggcgtc
     5881 atctagcgag tcgcctgcgc tccccggagc aatttctctc gcgcgccggg caatttggat
     5941 ggatagaaat ctccacgcca atgaagacac ttcctttcgt gctcgccgct ttcgcggccg
     6001 ccaccgtcct ggctcaagcc caggaaacca tccagcaacc agaccaggca aagctcgaag
     6061 cgatgaccgc ccgtttcgcg ccgaccgaaa tcacggcgga tgtctcccag ctctcgccta
     6121 acgatcgcaa agtgctcgcc aaactggtcg aggcctcgca aatcatcgac ggcattttcc
     6181 tgcgccagag ttgggcgggc aacccctcga tgctcctcga cctcgcgggt cgcgacagcg
     6241 ccgaggaccg cgcgcgcctc cattatttca ttctcaaccg gggcccgtgg tcgaacctcg
     6301 aacacaacgc gccctttgtt ctcggcgcgc cacagaagcc ggcaggcgcc ggtttttacc
     6361 cgccgaacgc gaccaaggtt gagatcgaag c
//
LOCUS       DATMNS010000047         8059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3249607, whole genome shotgun sequence.
ACCESSION   DATMNS010000047 DATMNS010000000
VERSION     DATMNS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3249607"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1075)
                     /locus_tag="VNW28_01820"
     CDS             complement(<1..1075)
                     /locus_tag="VNW28_01820"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HXA08687.1"
                     /translation="MACDQFHVIADYLNIDKNDRDRLMYPKRAMAVTLPVHMDDGTTQ
                     TFQGYRVQHHLTLGPTKGGTRFAPSLSMGETAALAMWMSWKCALANLPYGGAKGGVAC
                     NPTKLSVKELEAVSRRYMQEMIPFVGPHTDIMGPDMGTNEQVMAWFMDTYSVYQGYSV
                     GEIVTGKPVSIGGTEGRREATGRGVVYLIERAMDMLKIDPSKCTAIVQGFGNVGSVAA
                     LSLALKTGVKVVGISDAYVALYNPKGIDVKAAEQHVLKKGSLKAFNEGDRIDPNEMLI
                     MPCDVLVPAAIDRVINEKNAAQLKCRILAEGANGPTSPEADLILEKRWDEVFVIPDIL
                     CNAGGVIVSYFEWVQGLQSFLWSE"
     gene            1252..2391
                     /locus_tag="VNW28_01825"
     CDS             1252..2391
                     /locus_tag="VNW28_01825"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXA08688.1"
                     /translation="MSEPSFELHPETIAVHAGRTIDPATGAVVAPIHLSTTFQRGADG
                     TFPTGFDYSRTDNPNRAALEKGLALVEGGALAAAFPSGLAAAQAVFQALAPNDHVLAP
                     LEAYHGVLRLLRDIFTRWRLEVDFVDMTDLDAIKKGIRPKTKIIWVETPSNPTLKLTD
                     LAAAAALAHTAGARLVVDNTWCPMIQRPFALGADLVMHSTTKYFGGHSDVMGGALIAR
                     QDDEFFQRVREAQKTGGAVPAPFDCWLVHRGMQTLPWRMRAHCENAARVAQFLSAHPK
                     VERVHYPGLPSHRQHELAQRQMSLFGGMLSFELKSNRAAAMALPNRTQIFTRATSLGG
                     VESLIEHRQSIEGPDTLTPATLLRLSIGLEHPDDLIADLAQALAV"
     gene            complement(2444..4048)
                     /gene="murJ"
                     /locus_tag="VNW28_01830"
     CDS             complement(2444..4048)
                     /gene="murJ"
                     /locus_tag="VNW28_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXA08689.1"
                     /translation="MAEDQRVSTKATSVVGLAVLCSRVLGLIREMVIAGLFGASKNMD
                     AFLTAFRAPNMLRDLFAEGALSTAFVTTFSRRIATEGDQSAWRLASKVATLTVVFMSA
                     LTLLGIVFAPFVIGVLAPGFPVEKADLTIELTRIMFPFILLVSLAALVMGMLNAKNVF
                     GVPAMASSFFNIGSIVGGVAFCYWLDPQADWRHPHFGERGLVGLALGTLLGGLLQLVV
                     QFPSASRVGFRFRFDFNWRDPGVRTILALMGPATIAASAVQVNVAVNSGFASTLGNGP
                     ITWLNIAFRLMQLPLGVFGVAVATVTLPLVSRSAALGNAREFRGALAHAMRLVMLLTI
                     PSAIGLIILAHPIIAVIYQHGRFTADSTTQTAAALQFYAIGLAGYAGVKVLAPAFYAI
                     NKRHLPMVVSLISIGVNFALNWFFTFHLRLGHRGLALSTSFVAITNFLLLYGMMRRHT
                     GRLETGQMLATLGKVFLAGCVLAAVCLGAQRFIFPATHGSEWKLILAVAVTITVGAGA
                     FFGTAYLLRIAEVHDVVTIARRKLKL"
     gene            complement(4053..6638)
                     /locus_tag="VNW28_01835"
     CDS             complement(4053..6638)
                     /locus_tag="VNW28_01835"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HXA08690.1"
                     /translation="MFTPLEVFLLLGLGLGLVLFLLPLIRLGELRRTINTLTERVAAL
                     ERKLPRSETPPVPEKVAIPPSPLLAPRRVPDERKLVPPVTGQPPPLPPPLPATLPPAT
                     PFNWEAFMGVKLFAWLGGLALFLGVVFMVKYSFDNNLITPLGRLAIGGVVGAGLIATG
                     WWLARGRYRVTAQSLCATGIVILYANLFAAHSFYGLISLTTAFFAMSAVTLFSFLLAV
                     NLSAQVIVILGLLGGFLTPALLPSGADNAPALFLYIGLLDLGVAAVTLRKRWHYLVLL
                     AAIGTALTQLGWAIQFFNAARGGRVFWIFLGFEALFLAFYFCGRFALRSAGTSIDALT
                     PGNVNTRASHSEAATTAAAVVAGLSALGFGFWLSAVPALARQPLFFFSFVFLADAGLL
                     VLPVIGRAHRGIALGAGSLTFILVAEWIGLFAPHAPLIAALAAIVIFALLHAATVPAS
                     AWRPGVLPFTAAGTVTPFLLLILLLERVALWNPTPVFGVALILTGILLAAAIVKRADW
                     LALSALLGAGLVELTWQAVRFSNEQAIPALVCYGLFLICFVAFPFCSAQKEKVWSWAI
                     SALAQALQFWLFYRALSAAFPNDRMGLLPAVFIVPAAIGVVALLRLYQANPASGDARL
                     AWQGGALLLFVSLIFPLQFHREWITLGWALEGFALLWLFHRVPHRGLRLVGVLLLGGA
                     FARLALNPAVFAYHKRSTTRLWNWYLYADGITTACLLGGARVFGRPQETAVERAAPPL
                     LGSLGVVLAFLLLNIEIADYFSIGPTLTFSFTGNFARDMTYSIAWALFALGLILVGMR
                     IKQRAARYAGVALLGVTLAKLFLHDLSDLNELYRIGAFVSVALILIAASFIYQRFLVP
                     ERAAK"
     gene            complement(6660..7415)
                     /locus_tag="VNW28_01840"
     CDS             complement(6660..7415)
                     /locus_tag="VNW28_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HXA08691.1"
                     /translation="MTALAGLVRYADKFLRLTEIEDYSNALNGLQVENSGAVTKIGAA
                     VDASSRSIEMAIAQGIDLLVVHHGLFWPGLQPVTGPLYRALKLALEHNLALYSAHLPL
                     DLHPTIGNNVLLAAALGFGKTEPFLEMKGEPIGRKVEATLGRAELIAKLEESLGGPVR
                     CVGTGPMESKVIGIVTGGAGGEIHAAARAGVDSYITGEAPHWAAVAAEELGLNLFLGG
                     HYATETFGVKALAAELSRQFDVPWEFLDHPTGL"
     gene            7573..7923
                     /locus_tag="VNW28_01845"
     CDS             7573..7923
                     /locus_tag="VNW28_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08692.1"
                     /translation="MEYTDAPVAEGSEQPSHSPTAASGYLETAEGQASDLVAVMLSIV
                     PGLGHIYKGHRLVGMLLVFGTPIAVGLALLVATFTAGFGFFLLPIFWIAVMVNVWAIP
                     DRVKTDTAEEGEQY"
BASE COUNT         1757 a         2505 c         2504 g         1293 t
ORIGIN      
        1 tttcgctcca gagaaacgac tgcaatccct ggacccattc gaagtaagaa acgatcacgc
       61 cgccggcgtt gcagagaata tcgggaatga caaaaacttc gtcccatcgt ttttcgagaa
      121 tgagatccgc ttccggactg gtcgggccat tggcgccttc ggccaaaatc cggcacttca
      181 attgcgcggc gtttttctcg ttgatcacgc gatcgatcgc ggccggcacg agcacgtcgc
      241 acggcatgat cagcatttcg tttggatcga tgcgatcgcc ttcgttgaaa gctttaaggg
      301 atcccttttt caaaacgtgt tgctcggccg ctttcacgtc gattcctttg ggattataaa
      361 gagcgacata cgcgtcgctg atgccgacaa ctttgacgcc ggtcttgagc gcgagcgaaa
      421 gcgccgcgac cgagccgaca ttgccgaatc cttgcacgat agcggtgcat tttgaaggat
      481 cgatcttgag catgtccatg gcgcgctcga tcagataaac gacgccgcgg ccggtggctt
      541 cgcgtcgtcc ttccgttccg ccaatcgaaa ccggtttgcc ggtgacgatc tcaccgacgg
      601 agtaaccttg ataaaccgaa tacgtgtcca tgaaccaggc catgacctgt tcgtttgtgc
      661 ccatgtccgg gcccatgatg tcggtgtgcg gaccgacaaa gggaatcatc tcctgcatgt
      721 aacgccgcga gaccgcttcc aattctttca cggaaagttt cgtcggattg caggccacgc
      781 cgcctttggc gccgccgtaa gggagattag ccagcgcgca tttccagctc atccacatgg
      841 cgagcgcggc ggtttcgccc atcgagagac taggagcgaa acgcgtcccg ccttttgtcg
      901 ggccgagggt gagatgatgc tgcacccggt atccctgaaa agtctgcgtg gtcccatcgt
      961 ccatgtgcac cggcagggtg acggccatcg cgcgtttcgg atacatcagc cggtcgcgat
     1021 cgtttttgtc gatgttcagg taatcggcga tgacatggaa ctggtcgcag gccatctgaa
     1081 aaacttcgtc gtcgtatatc ataagaggcg gagccggacc gttagggtaa accggcggtg
     1141 gatgcagttg gtttttccgc tgggaccatc gagccgtcaa atgcaaatcg cgccttccgc
     1201 gacgcgcact ggcgatccgc cggtcacggc gttagtctcc gcgaacccgt tatgtccgaa
     1261 ccgtccttcg agctgcatcc cgaaacgatc gcggtgcatg ccggtcggac catcgatccc
     1321 gcaaccggcg cggttgtcgc gccaattcat ctttcgacca cattccagcg cggcgcggac
     1381 ggcacgttcc cgactggctt cgattattcg cgcaccgata atcccaaccg cgccgcgctc
     1441 gaaaaggggc tcgccctggt cgaaggcggc gcgctggccg cggcctttcc ttcgggcctc
     1501 gcggcggccc aggccgtttt ccaggcgctc gcgcctaacg accacgtcct cgccccgctc
     1561 gaagcctacc atggtgtcct gcgcctgctc cgcgacatct tcacccgctg gcggctcgag
     1621 gtcgatttcg tcgacatgac cgatctcgac gcgatcaaaa aaggcattcg cccaaagacc
     1681 aaaatcatct gggtcgagac gccttccaat ccgacgctaa aacttaccga cctcgcagcg
     1741 gcggccgcgc tcgcgcacac cgcaggagcg cgcctcgtgg tcgacaacac gtggtgcccg
     1801 atgatccagc gcccgtttgc gctcggcgcg gatctcgtca tgcattcgac caccaaatat
     1861 ttcggcggac acagcgacgt catgggcggc gcgctcatcg cgcggcagga cgacgagttc
     1921 tttcagcgcg tgcgcgaggc gcagaaaacc ggcggcgcgg tcccggcgcc gttcgattgc
     1981 tggctggtgc atcgtggaat gcaaacgctt ccctggcgga tgcgcgcgca ttgcgaaaac
     2041 gcggcgcgcg tcgcccagtt cctgagcgcg catccgaaag tggagcgcgt ccattatccg
     2101 ggtttgcctt cgcatcggca gcacgaactg gcccagcggc agatgagcct gttcggcgga
     2161 atgctttcct tcgagctgaa aagcaatcgc gcggcggcaa tggcgctgcc caatcgaacg
     2221 cagattttca cccgggccac gagcctcggt ggagtggaaa gcctgatcga acaccgccag
     2281 tcgatcgaag gcccggacac gctcaccccg gcgacgctcc tccggctctc gatcggcctc
     2341 gagcatcccg acgatcttat cgccgatctc gcgcaagcgc tggctgtgta gcggcgccca
     2401 ggccgggcgg aaaagtcagt cgcggcggcc ggcacagtcg ccgctacagc ttgagcttgc
     2461 ggcgcgcgat cgtgaccacg tcgtgcacct ccgcgatgcg caggagatag gccgttccaa
     2521 aaaaagcgcc cgctcccacc gtaatcgtga ctgcgacggc gagaatcaat ttccactccg
     2581 agccatgcgt ggccggaaag atgaaccgtt gcgcgcccag gcaaacggcc gcgagcacgc
     2641 accccgcgag gaacactttg cctaacgtcg ccagcatttg cccggtctcg agccggccgg
     2701 tatgccggcg catcatcccg tagagaagga ggaaattggt gatggcgaca aagctcgtcg
     2761 aaagcgcgag cccgcggtgc ccgaggcgga gatgaaaagt gaagaaccag ttcagggcaa
     2821 agtttacccc gatggaaatc agactaacga ccatcgggag gtgacgcttg ttgatcgcat
     2881 agaaggcggg cgcgagcact ttcactcccg cgtagcccgc caggccgatc gcgtagaact
     2941 gcagggccgc cgccgtctgt gtggtcgagt ccgcggtgaa acgtccatgt tgataaatca
     3001 ccgcaatgat cgggtgcgcg agaatgatca gcccgatggc ggatggaatc gtgagcagca
     3061 tgaccagccg catggcatgc gcgagcgcgc cgcgaaattc ccgggcgttg ccgagcgccg
     3121 cgctccgcga aacgagcggc agggtgacgg tcgccactgc cacgccaaag acgccgagcg
     3181 gcaattgcat gaggcgaaag gcgatgttca gccaggtgat gggaccgttg cctaaggttg
     3241 acgcaaaccc gctgttcacc gccacgttca cctgcaccgc gctggccgcg atcgtcgccg
     3301 gccccatcag cgcgaggatg gtgcgcacgc ccgggtcgcg ccaattgaaa tcgaaccgga
     3361 agcgaaagcc aacgcgggac gcggaaggaa actgcacgac caactgcaag agacctccga
     3421 gcaaagtgcc gagcgcaagc ccaaccagcc cgcgctcgcc gaagtgcgga tgccgccagt
     3481 cggcctgcgg gtcgagccag taacaaaagg ccacgccacc gacgatcgag ccgatgttaa
     3541 agaagctcga agccatcgcc ggaacgccaa aaacgttttt cgcgttcaac attcccatga
     3601 cgagcgcggc gagcgagacg aggaggatga agggaaacat gatccgcgtg agctcgatcg
     3661 tgaggtccgc cttttcgacc ggaaaaccgg gggccagcac gccgatgacg aagggcgcga
     3721 aaacaattcc gaggagcgtc aacgcgctca tgaaaacgac ggtcagggtt gcgaccttgc
     3781 tcgccagtcg ccaggcggat tgatcgcctt ccgtcgcgat gcgccgcgaa aacgtcgtga
     3841 cgaaagcggt cgagagagcg ccctcggcaa agagatcgcg caacatatta ggcgcgcgga
     3901 acgcggtcag gaacgcgtcc atgtttttcg aggcgccgaa gagaccggca atcaccatct
     3961 cgcgaatcaa cccgagcaca cgactgcaca aaaccgccag gccgacgacg ctcgtcgcct
     4021 tggtactgac gcgttggtcc tcggccatcg gcttattttg ccgcgcgctc gggaacaaga
     4081 aagcgctggt agataaaaga agccgcaatc aaaatcagcg ccacgctaac gaaagcgccg
     4141 atccggtaaa gctcgttcaa atcgctcagg tcgtggagga ataacttcgc cagggtcacg
     4201 ccgagcagcg cgacgccggc ataacgagcg gcccgctgct tgattcgcat gccgacgagg
     4261 atgaggccga gcgcgaagag cgcccacgcg atcgagtagg tcatgtcgcg ggcaaagttg
     4321 cccgtgaacg aaaaagtcaa cgtcggtccg atcgaaaaat aatcggctat ctcgatatta
     4381 aggagaagaa aggcgaggac cacgcctaac gaaccgagca acggcggcgc ggcgcgctcg
     4441 acggcggttt cctgcgggcg gccaaaaacc cgcgccccgc cgagcaaaca cgcggtcgtg
     4501 attccatcgg cgtagagata ccaattccag aggcgcgtcg tgctccgctt gtgataggcg
     4561 aagaccgccg gattgagcgc gagccgggcg aaggcgccgc cgagcagaag gacgccgacc
     4621 aggcgcagac cgcgatgcgg aacccggtgg aaaagccaga ggagagcgaa gccttccagc
     4681 gcccagccga gggtaatcca ttcgcgatga aactggagcg ggaaaatcag actaacgaaa
     4741 agcaggagcg ccccaccctg ccaggcgagc cgggcatccc cgctcgcggg attggcttga
     4801 taaagcctga gcaacgcgac cacaccgatc gcagccggga cgatgaagac cgccggcagc
     4861 agccccatcc ggtcgttagg gaaagcggcc gagagagcgc gataaaacag ccagaactgg
     4921 agcgcctggg cgagcgcgct gatcgcccac gaccacacct tctccttttg cgccgagcag
     4981 aacgggaagg cgacaaagca aatcagaaac agtccgtagc acacgagcgc ggggatcgcc
     5041 tgttcgttcg aaaaacgcac agcctgccag gttagttcca ccagaccggc gcccagcagg
     5101 gcggaaagcg cgagccaatc cgcgcgtttc acaatcgcgg cggccagcag aattccggtg
     5161 agaatgagcg cgacgccaaa aaccggcgtc ggattccaga gtgcgacgcg ttcgagcaaa
     5221 aggatcagga gcaggaaggg cgtcaccgtg ccggcggcgg tgaaggggag cacacccggt
     5281 cgccaggccg aagccggaac ggtcgcggcg tgcaggagcg cgaaaataac gatcgcggcg
     5341 agggccgcga tgagcggcgc atggggagca aacaatccga tccattccgc caccagaatg
     5401 aacgtcaggc tgccggcccc gagcgcgatc ccgcgatgcg cccggccgat cactgggaga
     5461 acgagtaaac cggcgtccgc caggaaaacg aagctaaaga aaaagagcgg ctggcgggcg
     5521 agcgcgggaa cggccgagag ccagaaaccg aatccgagag ccgagagccc ggcgacgacc
     5581 gcggcggccg tggtagccgc ttcgctgtgc gaagcacggg tattgacgtt gccgggagtg
     5641 agagcatcga tgctcgtccc cgcgcttcgc agagcgaagc ggccacagaa gtagaacgcg
     5701 agaaagagcg cttcaaaacc gaggaagatc caaaagacgc gaccgcccct ggcggcgttg
     5761 aaaaactgga tggcccagcc cagttgcgtc agagccgtgc cgatggccgc gagcagaacc
     5821 aggtaatgcc agcgtttgcg cagcgtcacc gccgccaccc cgaggtcgag caatccgatg
     5881 tagagaaaga gcgcgggcgc attgtccgcg ccactcggga ggagcgccgg ggtgagaaag
     5941 ccgcctaaca atccgagaat gacgatgacc tgcgcgctga ggttcaccgc gagaaggaac
     6001 gaaaagagcg tgaccgcgga catggcgaaa aacgccgttg ttagggaaat caatccgtag
     6061 aaggaatgcg ccgcaaaaag gttggcgtag agaatgacga tgccggtggc gcaaaggctt
     6121 tgcgctgtca ctcgatagcg accgcgcgcc agccaccacc cggtcgcaat caaaccggcg
     6181 ccgacgactc ccccgatcgc gagccggccg agcggcgtga tgaggttgtt atcgaaggag
     6241 tatttgacca tgaaaacaac gccgagaaaa agcgcgagcc cgcccaacca ggcgaacaac
     6301 ttcactccca taaaagcttc ccaattgaac ggcgtcgccg gtgggagcgt tgctggtaac
     6361 ggcggcggaa gcggcggcgg ttgacccgtt accggaggga cgagcttccg ctcgtccggg
     6421 actcgccgcg gcgcgagcaa tggcgacgga ggaatcgcca ccttctccgg cactggcgga
     6481 gtctcgctgc gcggcaattt tcgctcaaga gctgcaaccc gctcggtcag tgtgttgatc
     6541 gtccgacgca actctccgag gcgaatgagc ggcaggagga acagaacgag acccaggcca
     6601 aggccaagaa gaagaaagac ctcaagcggc gtaaacatca gttatcctta cgaatgcttt
     6661 cacaatccgg tgggatgatc gaggaattcc cacggcacgt cgaactgccg ggacagttcg
     6721 gcggcgagcg ccttgacccc gaaagtctcg gtcgcgtaat ggccgccgag gaaaaggtta
     6781 aggcccaact cttccgccgc gaccgccgcc cagtgcggcg cctccccggt gatgtagctg
     6841 tcgaccccgg cgcgcgccgc cgcgtggatc tcgccgcccg cgccgccggt cacgatgccg
     6901 atcaccttgc tttccatcgg ccccgtcccg acgcagcgga ccggtccgcc taacgactct
     6961 tcgagcttcg cgatcagctc ggcgcgcccc agcgtggctt cgaccttgcg gccgatcggt
     7021 tcgcccttca tttcgaggaa cggctccgtc ttcccaaaac caagcgccgc ggccaggagc
     7081 acgttgttgc cgatcgtcgg atggagatcg agcggcagat gagcgctgta gagggcgagg
     7141 ttgtgctcga gcgccagctt gagcgcgcga tagagcgggc cggtgactgg ctgcaacccg
     7201 ggccagaaca aaccatgatg cacgaccagc aaatcgatcc cctgcgcgat ggccatctcg
     7261 atcgagcgac tcgatgcgtc taccgcggcg ccgatctttg tcaccgcgcc cgagttctcg
     7321 acctgcaatc cgttgagcgc attcgaataa tcctcgatct ccgtcaggcg caaaaattta
     7381 tccgcataac gaacgagccc ggcgagtgca gtcatcccgc tactttgcag cttccgcgcg
     7441 gatttgaagt gcaaaaaagc gttcccaggc ggcgagcccc aaacggcgcc ggctggccgg
     7501 agcgaaaaac cgcaacccga tcttgacgaa gccgtgcccg atcgtgacga taggccagct
     7561 ggaggaaata ctttggaata cacagatgca cccgtcgcgg aaggatccga gcagccatcc
     7621 cattcgccga ccgcggcgag cggctatttg gaaacggcgg aaggccaggc gagcgatctg
     7681 gtcgcagtca tgctcagcat cgtccccggc ctcggccaca tctataaggg ccatcgactg
     7741 gtgggcatgt tgctggtttt cggaacgccg atcgcggtcg ggctggccct cctggtcgcc
     7801 accttcaccg ccggcttcgg tttcttcctt ctccccatct tctggatcgc ggtcatggtg
     7861 aacgtctggg cgatcccgga ccgggttaag acggatactg cggaagaggg cgagcagtac
     7921 taatgtagtc gcttcgctgc gcgaggcgtt gccccagacc gtttttgcga atgacgtcgc
     7981 ccctcgcgtt ttcccacagg gaaacggcta cagcgcggcg tgaatctccg cgaggatttc
     8041 gcgcagaccg taacggaaa
//
LOCUS       DATMNS010000048        13659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5069878, whole genome shotgun sequence.
ACCESSION   DATMNS010000048 DATMNS010000000
VERSION     DATMNS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 13659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13659
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5069878"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VNW28_01850"
     CDS             <1..731
                     /locus_tag="VNW28_01850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA08693.1"
                     /translation="DALKDFSLKLEPGKSYALVGASGAGKSTILSLILRLYDPTSGVL
                     RIDGRDLRSVTQKSLRQQIGLVSQETFLFHDTIFTNIQFGRLDATAEQIYAAAKTAYA
                     HDFIMAQPQGYQTIIGDKGCLLSGGQQQRIAIARALLKNAPILLLDEATSALDSESEQ
                     QIQLALEHLAEGRTVIAIAHRLSTILSADQIVVMEKGRIKEIGTHAELLENSSYYRRL
                     YDLQFNRNPEEPGAEVELLAEATI"
     gene            complement(782..1282)
                     /locus_tag="VNW28_01855"
     CDS             complement(782..1282)
                     /locus_tag="VNW28_01855"
                     /inference="COORDINATES: protein motif:HMM:NF020398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1802 family protein"
                     /protein_id="HXA08694.1"
                     /translation="MQSIGFKEWALVCAALGEGRQSVIIRKGGIAEGRDGFAFKHREF
                     FLFPTFFHEQLSQVRLARPTLPEPRPDEVEIRYFARVEEARLLTRWEDARALAPLHIL
                     AESVVRERFEYDETPGVHVAFVEIFRLAPVWRFPDAKEYGGCRSWVQLPEPPAGLQLQ
                     KVIAAG"
     gene            1312..2040
                     /locus_tag="VNW28_01860"
     CDS             1312..2040
                     /locus_tag="VNW28_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXA08695.1"
                     /translation="MPFKIDLHTHSFFSGDGVSSPEENIAAAKAKGLHGFALTDHNTN
                     DGVTYMLEKGLMRADGIPVDNFLVIPGVEVTTAEGHLLCLGASLPFLKGRPALEVCDL
                     IHERGGLAIPPHPYDLFRAGIRFSTLEKLPIDALEVFNAATTLRRYNRYAFKYAQVRG
                     LPMTAASDAHHHAAIGTAYTILNTEDFSVRGILAQILKSNELNQEYLTPKDSVRKTWN
                     NWMRLRRRKTLPNRSAAEVSKNGG"
     gene            complement(2150..4327)
                     /locus_tag="VNW28_01865"
     CDS             complement(2150..4327)
                     /locus_tag="VNW28_01865"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXA08696.1"
                     /translation="MTPTPPAVAAAPPAHVEHTNRLAREKSPYLLQHAHNPVDWYPWG
                     EEAFAKARREKKPIFLSIGYSTCHWCHVMERESFENEEVAAVMNAHFVNIKVDREERP
                     DVDRVYMTFVQATSGGGGWPLNVWLTPGLKPFVGGTYFPPEDRYGQPSFMKVLHRIAD
                     AWESDRAKISQSGDQIIAALAHSANTPAEKPGKVNAKVIEAAYQQIARGYDAREGGFG
                     GAPKFPRPVTLNFLFHVYHRSAPNSEEGEAALGMGLFTLRKMAAGGIHDQLGGGFHRY
                     SVDAVWHVPHFEKMLYDQAQLASSYVDAYQITGDLRYEGIARDTLDYVRRDMTDPGGG
                     FYSAEDADSLTTAGSGEKAEGAFYVWTKNEIDEALGEYGELFDYQYGVEADGNAPEGS
                     DPQGEFKGKNILYERQSFAETAKHFGRSEMDVDESMRYRRATLLEMRAKRPRPHLDDK
                     IITAWNGLMISAFARAAQVLGDTAYLEAATRAADFVRSKLYDEEAHTLARSYRGGPSG
                     IGGFAEDYAFYIQGLLDLYEASFDVQWLQLAEQLQATQDRLFWDDKNGGYFGSTGDDP
                     SILLRLKDDNDNAEPAPSSIAALNLLRLAQIRDSKELRARGEKTIAAFGASLDRFPSA
                     MPQLLVALDFSLTKPRQIIIAGQPGAPETRELIEEVYRHYLPDTIVLLADGGEGQKFL
                     AGKLEEMKEMKPVGGKAAAYVCEDFTCKAPVTTREELRKLLSR"
     gene            complement(4571..5053)
                     /locus_tag="VNW28_01870"
     CDS             complement(4571..5053)
                     /locus_tag="VNW28_01870"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HXA08697.1"
                     /translation="MNKGQRISRLVGELGGASAERARDEIAQHPCYLGFFRCWNEQRY
                     YEAHDVLEHLWLQTESEDANFFKGLIQAAGGFVHLQKQYARPTHPKDGRRMYPAVRLF
                     RLAEKNLQPFAPIRHRLDVSKLLALLGAQTNAVTKGHYRANPWSPETAPSLHLLPGSD
                     "
     gene            5149..6441
                     /locus_tag="VNW28_01875"
     CDS             5149..6441
                     /locus_tag="VNW28_01875"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXA08698.1"
                     /translation="MTAVGLTLLLAAIVLLISCSALFSGLETALFALKSHQIQRLQEQ
                     HPSLADFLGTFRNNPRRVLNVLLLGDALVNVPLIILCLVLIWEGPLARHLPAWIAALT
                     IFAVVVLLCDLIPKLLALSSAYRLSAVGVFTLKAMMPLLDRVGQLLENASTGIIDHLT
                     PVHLRVRPRLSDEELETLVEMGEEEGTLHEAEGEMIQEIIKLGDKTAKDCMTPRVDTF
                     AVPDDLPNEEAIALFQQRRHRRVPVYAETPDQIAGIVEVKQFLLDPSQHYSETLIPPS
                     FVPETMRAMDLLRSFLSHPQGLAIVVDEFGGTEGIITLEDIIEEILSDAAPLGDADLY
                     IEPLEDGRMLVSGNARLDDLTEHLGFELTAEGIDTIGGYVFNRLGYLPKAGATLEIPH
                     LAITVRRVSRKRIEEVLLEKTGTGAGEDAAAADTDPGV"
     gene            6438..7385
                     /locus_tag="VNW28_01880"
     CDS             6438..7385
                     /locus_tag="VNW28_01880"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HXA08699.1"
                     /translation="MTPWFIIFFCGVVSFVFSGIEAGLLSLDPVRLRHQVKLRNRSAI
                     LLDRLMKNPERLLATVLLVTNFVDIAGLLVLTRRLVLLFGTGGFLVALLVAGPIYLFL
                     LGVLPKSLFRRFPYRALAFFAGLLVFTSRLLWPVLELGSQTGRLLFRREKAPLRLFAA
                     REDLKQLAVESERQGALTSTERAMIHNVVDFRTVKVSAVMVPLSKIISVPPETTIPQL
                     LKLSAQTGIDRVPVIAGNGSALGLVNVLDVLLDPTPSHELRSYMRRIVSAADNESAYR
                     IIRRLRAARLTLAAVFDGREKLIGIVTVEDLVRRLVQSA"
     gene            complement(7401..7913)
                     /locus_tag="VNW28_01885"
     CDS             complement(7401..7913)
                     /locus_tag="VNW28_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08700.1"
                     /translation="MKRQLFSWALLACIATGALVLSGCSTVSSRIESNRSSFDQLSPK
                     DQAMVSQGQINGGMSQAAVYIAWGQPQQKAVGQVHGVSTESWVYTLSTAAYGPYGYGY
                     GGFGYGWGYAGPIGFYGRHGGHRFYGAFFDPYWDPYYYPFAYTVSYPVKTVSFQRGRV
                     VAFQYLAPGY"
     gene            8095..9096
                     /gene="moaA"
                     /locus_tag="VNW28_01890"
     CDS             8095..9096
                     /gene="moaA"
                     /locus_tag="VNW28_01890"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HXA08701.1"
                     /translation="MEDRFGHRISYLRVSITDRCNERCTYCMPHELQEWLPRHDILTF
                     EETIRLIRIAGELGVTKIRLTGGEPLTRRDVMQLFRQLPRLTNLRDIGLSTNGTLLTR
                     EVEPGLTMAAAVRACGFNSVNISLDTLDREEYARVTGRDLLPEALAGIDAAVAAGFLQ
                     VKINCVLMRGRNQEALAPLVDFAAERGLLLRFIELMPVSTTEVLTESNFFPVAEAKRT
                     IEAKFGSLIPQPEFRTNGPASYYQIPGRAQRIGFIGAMTNLHFCESCNKLRLTCDGKL
                     RPCLGSYLEFDIMKPLRAGASDEELRQFFLEVVERKPEQHDFRTTYQPGRKMVAIGG"
     gene            9100..9570
                     /gene="moaC"
                     /locus_tag="VNW28_01895"
     CDS             9100..9570
                     /gene="moaC"
                     /locus_tag="VNW28_01895"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXA08702.1"
                     /translation="MDRLTHIASDGSAKMVDVSAKPLSHREAIASGRIELQPATLELI
                     ASDRIAKGNVFATARIAGIQAAKQTARLIPLCHTLGLSHVELGLTSSATGVEATCTAR
                     TTAQTGVEMEALTGVTVALLAIYDMCKAVDKQMRIGDVHLVRKTKTAARQDLQD"
     gene            9654..10136
                     /locus_tag="VNW28_01900"
     CDS             9654..10136
                     /locus_tag="VNW28_01900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HXA08703.1"
                     /translation="MQITVGIITVSDRASRGEYADLGGPALKEVALKNNWSVLCEAIV
                     PDEIERLQEAIRSFADQGCGLILTTGGTGLAARDITPEAIRGLMRVELPGFGEVMRAE
                     SMKITPNAILSRNLAAIVAQSLVIALPGKPSGAVECLGFVAGAIPHGVALAQRLPTSC
                     "
     gene            10199..10846
                     /locus_tag="VNW28_01905"
     CDS             10199..10846
                     /locus_tag="VNW28_01905"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HXA08704.1"
                     /translation="MKNKNLVAALSLCVLLSTGCDWRGIRGNGQIKTETRPVTAFTRV
                     EAGGFYELEWRPGAPSFSLTTDENLLSQITTSMEGDLLKIKTHDSIAPTHGIKVVITS
                     SSLSGASLSGAVKLDAAQLSGATFALETSGATKVTLAGKVNRLLASLTGASKLQAADL
                     SADDVELSVTGAGKADVTATNLLRAAITGAGKVSYGGNPKSVEKKISGAGKIEPR"
     gene            10965..11657
                     /locus_tag="VNW28_01910"
     CDS             10965..11657
                     /locus_tag="VNW28_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HXA08705.1"
                     /translation="MNSEPAFHLFGQAHLVVIFLTVIIPVGLGVAVRLTGARGLDRAV
                     AYGLSLLLATNYFGYAFYLLSHHLLVWQQALPFQLCDWAMVTIIVALLTARRSWTEVS
                     YFWGIGGTFQAILTPNLQVGFPDIRFISFFIGHCGIVAGVIFLMIARRFRPTLGSVWR
                     TLAWSQLYLVATLAVDYLTGDNYGFLLHKPQAASTLDYLSTTRGLYILELEGLALVFF
                     ALLYAPFAIKTR"
     gene            complement(11670..12395)
                     /locus_tag="VNW28_01915"
     CDS             complement(11670..12395)
                     /locus_tag="VNW28_01915"
                     /inference="COORDINATES: protein motif:HMM:NF018983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016977 - chitosanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 75 protein"
                     /protein_id="HXA08706.1"
                     /translation="MAKLKFLKRVGYSDINTLQDDDSAFFYESGMMIDADGGYHAYHP
                     DNISGLDYLGNAGKPGNWWALVTDNGKPNGNPVIQAKSDPAKGFYISTTSLEDTSMDA
                     KDPRRYVDAESVNFFVLPGRLGLGNKLGDFGVVIRPDKNDYDYAVYADVGPGSKIGEA
                     SIALAASLGIPSSPKTGGTGHGIIYIVFPDSAKGWPLSQAQIDQYAAALFATWGGLEK
                     AKDCFPGVNWLSGAPLIEIATPA"
     gene            complement(12521..12850)
                     /locus_tag="VNW28_01920"
     CDS             complement(12521..12850)
                     /locus_tag="VNW28_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08707.1"
                     /translation="MSIDTSDLIQDMLAAATGAAKGHAADLKSYLEARAKLVADGMVE
                     LEADRVANNITDDDVKFAFQQIKDSEMTSLLAIDVTLKAAAQDAINAALAVASDALNK
                     AVGFALL"
     gene            complement(12854..13297)
                     /locus_tag="VNW28_01925"
     CDS             complement(12854..13297)
                     /locus_tag="VNW28_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08708.1"
                     /translation="MSRLIKAKLPILFLAAVLFAGCTLVAPYDETTDHLLTDLSVKTE
                     TAIAQADAGQLSTSDREKFFDEAIGAVRAMKARSSLFDKNQDEVKAFSELEQRYQDLR
                     QHGGPPRSSLATGLRATLLDLQQIQIAKKRSSVFSAGLKKTNSNQ"
     gene            13366..>13659
                     /locus_tag="VNW28_01930"
     CDS             13366..>13659
                     /locus_tag="VNW28_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08709.1"
                     /translation="MVADVKTDVAGDLEKLLEAVDRAAKEIRPFAALELRIAAAVKVD
                     HLADIGVIESSKRDARNEIFIVYEAKGLAAEEVVVIAAKTKQTDAWLAESVDAV"
BASE COUNT         2638 a         4368 c         3912 g         2741 t
ORIGIN      
        1 atgacgcttt aaaggacttc agcctcaagc tcgaacccgg caagagttac gccctcgtcg
       61 gcgcgagtgg agcgggcaag agcaccatcc tttctctcat tttgcgcctt tacgatccga
      121 cgagcggcgt gttgcggatc gatggccgcg atctgcgctc ggtcacgcag aaatcgctgc
      181 gccaacaaat cgggctcgtt tctcaggaaa ctttcctttt ccacgacacc atcttcacca
      241 acatccagtt cggccggctt gatgcgaccg cagaacagat ctacgccgcg gccaaaaccg
      301 cttacgcgca cgacttcatc atggcgcagc cgcaaggcta tcagactatc atcggcgata
      361 agggctgtct cctctccggc ggccagcagc agcggatcgc gatcgcgcgc gctttgctta
      421 agaatgcgcc catcctgctt ctcgacgaag ccacctccgc gctcgattcc gaatcggagc
      481 aacaaattca gctcgccctc gaacacctcg ccgaaggccg caccgtaatc gcgattgcgc
      541 atcgcctctc caccattttg tccgccgacc agatcgtggt gatggagaaa ggccggatca
      601 aggaaatcgg cacgcacgcg gagttgctcg agaattccag ctactatcgg cgcctctacg
      661 acctgcagtt caaccgcaat cccgaagagc cgggcgcgga agtggagttg ctggcggaag
      721 ctacgatcta gtctggaacg cgtcggagcc ttgctggggt agcgcactcg ggacgagagc
      781 gctatccagc ggcaatcacc ttctgcagtt gcagtccggc cggcggctcg ggcaattgca
      841 cccaactgcg acaaccgcca tactcttttg catccggaaa acgccagacc ggagcgaggc
      901 ggaagatctc cacaaaagcc acgtgcaccc cgggagtctc atcgtactcg aagcgttccc
      961 gcacgacgct ttccgccagg atgtgcaacg gcgccagcgc gcgcgcgtcc tcccaacggg
     1021 tcaggaggcg ggcttcttcc acccgggcga aataacgaat ttccacttcg tcaggtcgcg
     1081 gctccggcag cgtcggacgg gccaaacgaa cctggctcag ttgctcgtgg aaaaacgttg
     1141 ggaaaagaaa gaactcccga tgcttgaaag cgaaaccgtc gcggccctcc gcgatgcccc
     1201 ctttgcgaat gatgaccgac tgtctcccct cgcccagcgc ggcgcagacc agcgcccatt
     1261 ccttgaagcc gatgctttgc atttcgcggc tcgcaaacta cgctcaggtc aatgcccttc
     1321 aagatcgacc tgcacacgca ttcgttcttt tcgggcgacg gcgtttccag ccccgaggag
     1381 aacatcgcgg cggccaaagc gaaagggctc cacggcttcg ccctgaccga tcacaacacc
     1441 aacgatggcg tgacctacat gctcgaaaaa ggcctgatgc gcgcggatgg gataccggtg
     1501 gacaatttcc tcgtcatccc aggtgtcgag gtgaccacgg cggaagggca cctgctctgt
     1561 ctcggcgcct cacttccctt tctcaaaggc cggcccgccc tggaagtatg cgatctcatc
     1621 cacgaacgcg gcgggctcgc catcccgcct catccctacg atttgtttcg cgccggcatc
     1681 cgcttctcga cgctcgagaa gctgccgatc gacgcgctcg aggtctttaa cgccgccacc
     1741 accctgcgcc gctacaatcg ctatgccttc aagtatgcgc aggtccgcgg cctgccgatg
     1801 acggcggcca gcgacgcgca tcatcacgcg gcgatcggaa ccgcctacac gattctgaac
     1861 acggaggatt tttcggtccg cggcatcctc gcccagatct tgaaaagcaa cgaactgaac
     1921 caggagtacc tgacgccgaa ggacagcgtg cgcaaaacct ggaacaactg gatgcggctg
     1981 cgccggcgca agacgctgcc gaaccggtcg gcggccgaag tatccaaaaa cggcggctaa
     2041 agaaaatgtg gggaaggact ctatgccccc gaaagcgaaa gccctctccg tcagccacgc
     2101 cgctgatcgc cgcaccaagg cggctcccac tttaggctcc cacacgtgcc taacgactca
     2161 aaagtttgcg cagctcttcc cgcgtcgtga ccggcgcctt gcaggtgaag tcttcgcaaa
     2221 cgtaagccgc cgccttcccc ccgaccggtt tcatctcctt catttcctcc agtttcccag
     2281 cgaggaattt ctggccttcg ccgccatcgg ccaggagcac gatcgtgtcc ggcaggtagt
     2341 gccggtacac ttcctcgatc agctcgcgcg tctcgggcgc cccgggttgc ccggcgatga
     2401 taatctgtct tggtttcgtt aggctaaaat cgagcgcgac cagcagctgc ggcatggcgc
     2461 ttggaaaacg atcgagactc gcgccgaagg cggcgatcgt tttctccccg cgcgcgcgca
     2521 gttccttgga atcgcggatc tgcgcgagcc ggagcaaatt caaagccgca atcgaactcg
     2581 gggccggttc cgcgttgtcg ttgtcgtcct tcagccggag caggatgctc ggatcgtcgc
     2641 ccgtgctgcc aaagtagcct ccatttttgt cgtcccagaa aagccggtcc tgggtcgcct
     2701 gcaattgctc tgccagctgg agccactgca cgtcgaacga ggcttcgtag aggtcgagca
     2761 gcccctgaat gtaaaaagcg tagtcttccg cgaaaccgcc gatcccggac gggccgccgc
     2821 gatagctgcg ggccagagtg tgcgcctctt cgtcgtacag ttttgacctc acgaaatcgg
     2881 cggcccgggt cgcggcctcg agataggccg tgtcgcccag gacctgtgcc gcgcgggcaa
     2941 aggcggaaat catcaatccg ttccaggcag tgatgatctt gtcgtccaga tgcgggcgcg
     3001 gccgtttcgc gcgcatttcg agcagagtcg cgcggcggta gcgcatcgac tcgtcgacat
     3061 ccatctcact tcttccaaag tgtttggctg tctcggcgaa ggattgccgc tcgtagagga
     3121 tgttcttgcc cttgaattct ccctgcgggt cgctcccttc gggcgcgttg ccgtccgcct
     3181 ccacgccgta ttggtaatcg aagagctcgc catactcgcc cagcgcctca tcgatctcgt
     3241 tcttcgtcca gacgtaaaac gcgccctccg ccttctcgcc gctgcccgcg gtcgttaggc
     3301 tatcggcatc ctcggccgaa taaaatccgc cgcccggatc ggtcatgtcg cgccgcacat
     3361 agtcgagcgt gtcgcgtgcg attccctcgt aacgaagatc gcccgtgatc tggtaggcgt
     3421 cgacgtagga gctggccagt tgcgcctggt cgtagagcat tttctcgaag tgcgggacgt
     3481 gccacaccgc atccaccgag tagcgatgga atccgccgcc gagttggtcg tgaatccccc
     3541 cggcggccat tttgcggagg gtgaacagcc ccatcccgag cgccgcctcg ccttcctcgg
     3601 agtttggggc agagcgatga tagacgtgga aaagaaaatt cagcgtcacc ggccgcggga
     3661 atttcggagc gccgccgaat ccgccctctc gcgcgtcgta gccgcgggca atttgctgat
     3721 aggccgcctc gatcaccttg gcgtttacct tccccggctt ttccgccggc gtgttcgcgg
     3781 agtgagccag cgcggcgatg atttggtcgc cgctctggct gatcttggcg cggtcgcttt
     3841 cccatgcatc cgcgatgcgg tgcagcacct tcataaaact cggctgcccg taacgatctt
     3901 ccggtggaaa ataagtgccg ccaacaaacg gtttcagccc cggggtcaac cagacattga
     3961 gcggccagcc gccgccaccg ctcgttgcct ggacaaaagt catgtaaacg cgatcgacat
     4021 ccgggcgttc ttcgcggtcg accttgatgt tgacgaagtg cgcgttcatc accgcggcga
     4081 cttcttcgtt ctcaaacgat tctcgctcca tcacgtggca ccagtggcag gtggagtaac
     4141 cgatcgagag aaaaatcggc ttcttctcgc gccgcgcctt ggcgaaggcc tcctcgcccc
     4201 atggatacca atcgaccgga ttgtgcgcgt gctggagcag gtagggtgat ttctcgcgcg
     4261 cgagccggtt ggtgtgctcg acgtgcgcgg gcggcgcggc ggcgacggcg ggtggagtcg
     4321 gtgtcacatc ctaccctacc gaaaaagcgc cggcacgcga aagggcaaga tcgcagggtc
     4381 ccgcgcaact cgatcgccgg ccgcgctggc tatttcatcg cgaaccggtc cgtttcttcg
     4441 ctcggtctgc gcactggccg gggccgcgat cattggcggt gcgtccgcct ctcatttcgg
     4501 gagccggcga cttcctgcgc acacgatttc gctcgtcctc gcgatcgtcc cggtaatcgc
     4561 cgaaacgaaa ttaatcgctg cccggtaaca ggtgcaggga cggcgccgtt tcgggcgacc
     4621 agggattcgc gcggtagtgg cctttcgtga ccgcgttcgt ctgcgcgccc aggagcgcga
     4681 gcaatttaga cacgtccagc cgatgtcgta tgggagcgaa cggttgtaaa tttttctccg
     4741 ccagccgaaa caaacgcacc gccgggtaca tccggcgccc atccttcggg tgggtcggcc
     4801 gcgcatattg cttctgcaaa tgcacaaacc cgccggccgc ctggatgagc cccttgaaaa
     4861 aattcgcgtc ttccgattcc gtctgcagcc agagatgctc gagcacgtcg tgcgcttcgt
     4921 aatagcgctg ctcgttccag caacgaaaga agccgaggta gcagggatgc tgtgcgatct
     4981 cgtcccgcgc acgctccgct gacgcgccgc ctaactcgcc caccaaccgg gaaattcttt
     5041 ggcctttgtt cacggaaagc ttagtctcgt ttcggcagag atgaccggca accgagcacg
     5101 cattattttt ccaagggagg cgggtgcgcg caccgccgca ggggacggat gacggcggtc
     5161 ggactcacgc ttctccttgc cgcgatcgtc ctcctgattt cctgctcggc gcttttctcg
     5221 ggcttggaga cggcgctttt tgcgctcaaa agtcaccaga tccagcgact gcaggagcag
     5281 catccctcgc ttgccgattt cctcggaacc ttccgcaaca atccccgtcg cgtcctcaac
     5341 gtcctcctcc tgggcgacgc gctggtcaac gtcccgctga tcattctctg cctcgttctc
     5401 atctgggaag gcccgctcgc ccggcatctg ccggcctgga tcgccgcgct cacgattttc
     5461 gcagtcgtcg tcctgctctg tgatctgatc cccaagttgc tcgccctctc cagcgcctac
     5521 cgtctctcgg cggtcggggt cttcactctc aaggccatga tgccgctgct cgatcgcgtc
     5581 gggcagttgc tcgaaaacgc cagcaccggg atcatcgacc acctcacgcc ggtccacttg
     5641 cgggtgcggc cgcggttgag cgacgaggaa ctcgagaccc tcgtcgagat gggcgaggag
     5701 gaaggcacgc tgcacgaagc ggagggcgaa atgattcagg aaatcatcaa actcggggac
     5761 aaaacggcca aggattgcat gacaccgcgg gtcgatacct tcgcggtgcc ggatgatttg
     5821 cctaacgaag aggcgatcgc gctcttccag cagcggcggc accggcgcgt gcccgtctat
     5881 gccgagacgc ccgaccagat cgccgggatc gtcgaggtga agcaattcct gctcgatccg
     5941 tcgcagcact acagcgagac gctcatccca ccgtcgttcg tccccgagac aatgcgggcg
     6001 atggatttgt tgcgcagttt cctctcgcac ccgcagggac tcgcgatcgt ggtcgacgag
     6061 tttggcggga ccgagggcat catcaccctg gaggacatca tcgaggagat cctgagcgat
     6121 gccgcgccgc tgggcgacgc cgacctttac atcgagccgc tcgaggacgg gcgcatgctc
     6181 gtgagcggca atgcgcgact cgacgatctc accgagcatc tcggtttcga gctgaccgcc
     6241 gaaggcatcg acaccatcgg aggatacgtt ttcaatcggc tgggttattt gccaaaagcg
     6301 ggcgccacgc tggaaatccc gcacctcgcg atcaccgtcc ggcgggtgag tcgcaaacgc
     6361 atcgaggaag tgctcctcga aaaaacagga acaggcgccg gggaggatgc ggcggcggcg
     6421 gacactgatc ctggcgtatg actccctggt tcattatttt tttctgcgga gtggtgtcgt
     6481 tcgttttctc cggcatcgaa gcggggctgc tttcgctcga cccggtgcgc ctgcgccacc
     6541 aggtgaagct gcgcaatcgc tccgccattc tcctcgatcg cttgatgaaa aacccggagc
     6601 gactccttgc caccgttttg ctggtgacga attttgtcga tatcgccggg ttgctcgtac
     6661 ttacccggcg gctggtcttg ttgttcggca cgggaggctt cctcgtcgcg ctcctcgtcg
     6721 ccggaccgat ctatctcttc ctcctcggcg tcctgccgaa atcgctcttc cgccgcttcc
     6781 cttatcgcgc gctcgctttc ttcgccgggc tcctcgtctt cacttcccgc ctcctttggc
     6841 cggtcctgga actcggttct cagaccggtc ggctcctctt ccgccgcgaa aaggcgcccc
     6901 tccggctttt cgccgcgcgc gaggacttga agcagcttgc ggtcgaaagc gaacgccagg
     6961 gcgcgctcac ctcgacggaa cgcgcgatga tccacaacgt ggtcgatttt cggacggtca
     7021 aggtctccgc ggtgatggtg ccgttgtcga aaatcatctc ggtgccaccg gagacgacga
     7081 ttccgcaatt gctcaagctg agcgcgcaaa cgggcatcga tcgggtgccc gtaattgccg
     7141 ggaacgggag cgcgcttggc ctcgtcaatg tgctcgacgt cttgctcgac cccactccgt
     7201 cacacgaact gcgctcctac atgcggcgca tcgtttccgc ggccgacaac gaatctgcct
     7261 accgaatcat ccggcggctg cgcgcggccc gtctcaccct ggccgccgtc ttcgacggcc
     7321 gcgaaaagtt aatcggcatc gtgacggtgg aggatcttgt caggcgcctg gttcagagcg
     7381 cctgatcatg attcggggct tcagtagccc ggcgctagat attggaaggc gacaactcga
     7441 ccacgctgaa aggaaaccgt cttcaccgga tagctcaccg tgtaggcgaa ggggtagtag
     7501 tacggatccc aataaggatc gaagaacgca ccgtaaaaac gatgaccacc atgccgtccg
     7561 taaaatccaa tcggaccggc ataaccccag ccataaccaa atccgccata accataaccg
     7621 tagggcccgt aggcagccgt cgaaagggtg tagacccagg actccgtcga caccccgtgc
     7681 acctggccca ccgctttctg ttgcggctga ccccacgcga tatagaccgc ggcctgcgac
     7741 atcccaccgt tgatttgccc ctggctcacc atcgcctgat ctttcggcga gagttgatca
     7801 aacgacgagc gattcgactc gatcctcgac gagacggtgc tgcaacccga gaggaccaac
     7861 gcgccagttg cgatgcacgc caacaacgcc cacgaaaaca attgacgctt catgcttgaa
     7921 tataaaacgc ggcgccacag cgtcaaactg gggcgggcgg atgagacatt caaaaaagta
     7981 acaacttcga aagaaatggc acctgatcga gttgcccgct tcccgacgcg acgattcaac
     8041 gtctcgccgg ccgcgccatc ttgattcgcg aaaggccccg ccgtagcctc ggtcatggag
     8101 gatcgcttcg ggcatcgcat ctcgtacttg cgcgtttcca tcaccgaccg ctgcaacgaa
     8161 cgctgcacct actgcatgcc ccacgagttg caagaatggc tcccgcgcca tgatatcctc
     8221 accttcgaag agaccatccg tctcatccgc atcgcgggcg agctgggcgt gacgaagatc
     8281 cgtctcaccg gtggtgagcc cctcacccgg cgcgatgtca tgcagctctt ccgccagctc
     8341 ccgcgcctaa cgaacctgcg cgacatcggc ctttcgacca atggcactct cctcacccgt
     8401 gaggtggagc ccggcctgac aatggcggcg gcggtccgcg cctgcggctt taattccgtt
     8461 aatatctcgc tcgacacact cgaccgcgaa gagtacgccc gtgtcacggg gcgcgatctg
     8521 ttgccggagg cacttgcggg gatcgacgcg gccgtcgccg ccggatttct gcaggtgaag
     8581 atcaactgcg tcttgatgcg cggccgcaac caggaggcgc ttgccccgct ggtcgatttc
     8641 gcggcggaac gcggtcttct cctccgcttc atcgaactca tgccggtgag cacgaccgag
     8701 gtcctgaccg agagcaattt cttccccgtc gccgaagcga aacgaacgat cgaagcgaag
     8761 tttggttcct tgattccgca gccggaattc cggaccaacg gcccggccag ctactatcaa
     8821 atccccgggc gcgcccagcg catcgggttc atcggcgcga tgaccaacct gcatttctgc
     8881 gagagctgca acaaactgcg cctcacctgc gacgggaaac tgcgaccctg tctcggcagt
     8941 tatctcgagt tcgatatcat gaagcccctc cgcgcgggag cgagcgacga ggagttgcgg
     9001 caattttttc tcgaagtcgt cgagcgcaaa ccggaacagc acgatttccg caccacttat
     9061 caacccggac ggaagatggt cgcgatcggc ggctgaggga tggatcggct gacccatatc
     9121 gcgagtgacg ggagcgcgaa gatggtcgac gtctcggcca aaccgctcag ccaccgggaa
     9181 gcgatcgcca gcggccggat cgaacttcag ccggcgacgc tcgaactgat cgcctcggat
     9241 cgcatcgcca aaggcaatgt cttcgcgacc gcgcgaatcg ccgggattca ggccgcgaag
     9301 caaaccgcgc ggctcattcc gctctgccac accctcggcc tgagccacgt cgagcttggc
     9361 ctaacgagca gtgccaccgg cgttgaagcg acctgcaccg cgcgcaccac cgcccagacg
     9421 ggcgtcgaga tggaagcgct caccggtgtg acggtcgcgc tcctcgcgat ctacgacatg
     9481 tgcaaagcgg ttgataagca gatgcgaatc ggcgacgtgc acctcgttcg caagacgaaa
     9541 acggcagcaa gacaggattt acaggactaa ctggatggga gaggaaaccg cattcccttc
     9601 ttcttccttc ttctaattcc aatcctcata atcctgtaaa tccggtcaaa aaaatgcaga
     9661 tcaccgttgg catcatcacc gtttccgatc gcgccagccg cggcgagtac gccgatctcg
     9721 gcggccccgc cttgaaggag gtcgctctca agaacaactg gagcgtcctc tgcgaggcga
     9781 tcgtcccgga tgaaatcgaa cgcctccagg aagcgatccg ctccttcgcc gaccagggtt
     9841 gcggcctcat cctgacgacg ggcggcaccg gtctggccgc tcgtgatatc acgccggaag
     9901 cgatccgcgg cctgatgcgc gtcgagcttc ccggtttcgg cgaagtcatg cgcgccgagt
     9961 cgatgaagat tacgccgaac gcgatccttt cgcgcaacct ggccgcgatc gtcgcgcaat
    10021 ctttggtcat tgccttgcct ggcaaaccga gtggcgcggt cgagtgcctc ggcttcgtcg
    10081 cgggggcgat cccgcacggc gtggcgctcg cccaacgcct gccgacatcc tgttaagccc
    10141 ggcccctctt ttttgaatcc aaaaccatcc gtcgtcgcat ctcaatggag atcaaatcat
    10201 gaaaaacaag aatcttgtcg ctgcgttgtc gctttgcgtc cttctctcca cgggttgcga
    10261 ttggcggggt atccgcggta acggccagat caagactgag acccggccgg taaccgcgtt
    10321 cacgcgggtg gaggcgggcg gtttctacga gctggaatgg cgtccgggcg ccccttcctt
    10381 tagcctaacg acggacgaaa acctgctttc gcagatcacg accagcatgg aaggcgacct
    10441 gctcaagatc aagacgcacg actcgatcgc tccgacgcac ggcatcaaag tggtcatcac
    10501 cagttcgtcg ctctcggggg ccagtctcag cggcgcggtg aagttggacg cggctcagct
    10561 aagcggcgcg accttcgctt tggagaccag tggcgcgacc aaggtaaccc tcgccggaaa
    10621 agtaaaccgg cttctcgcca gcctgacggg cgccagcaag ttgcaggcgg ccgatttgtc
    10681 ggcggacgat gtcgaattgt cggtcactgg cgccggcaag gcggacgtaa cggcgaccaa
    10741 tttgcttcgc gccgccatca ctggcgccgg caaagtcagt tacggcggca atccgaaatc
    10801 cgtcgaaaag aagatctcgg gcgcgggcaa gatcgagccg cgttagtagc tcggagggcc
    10861 atgcttccgc atgttctgga cgcgcggaag cgcatctccc gctacactcg cagagcttga
    10921 cgcgttaccc ggcaaggcgg aacaatcgcg ctgcgaccta cgccatgaat tccgaacctg
    10981 cctttcacct cttcggtcag gcccatcttg tcgtcatttt cctaacggtc atcatcccag
    11041 tcggactcgg agtcgcggtg cgcctgaccg gcgcgcgagg tctcgatcgc gcagtcgcct
    11101 acggcctctc attacttctc gccaccaatt actttggcta cgccttctac ctcttgagcc
    11161 accatcttct cgtctggcaa caggcgcttc ccttccagct ttgcgactgg gcgatggtta
    11221 ctatcatcgt cgcactgctg accgcccgcc ggagctggac cgaggtttcc tacttctggg
    11281 gaatcggcgg caccttccaa gccatcctga ccccaaatct gcaggtcggc ttccctgata
    11341 tccgtttcat tagcttcttc atcggccact gcgggatcgt cgccggcgtc atcttcctca
    11401 tgatcgcccg ccgtttccgc ccgacactcg ggtcggtctg gcgcaccctg gcgtggtcgc
    11461 aactctacct cgtcgccacc ctcgccgtcg actatctgac cggcgacaac tacggcttcc
    11521 tcctgcacaa accccaagcc gcctcaaccc tggattatct ctcgaccacc cgtgggctct
    11581 acatcctgga attggaaggg ttggcgctag tcttcttcgc gctcctctac gcgccttttg
    11641 cgatcaagac tcggtaggcc gcggctatct cacgccggcg tggcgatttc gatcagtggc
    11701 gcaccagaga gccagttcac gccgggaaaa cagtccttcg ctttctccag gccgccccag
    11761 gtcgcgaaca acgcggcggc gtactgatcg atctgagcct gactcagcgg ccaacccttc
    11821 gctgaatcag gaaacacaat gtagatgatc ccatggcccg tgccgcccgt cttcgggctg
    11881 gagggaatgc cgagcgacgc agccaaggca attgaagctt caccaatctt gctgcccggc
    11941 ccgacatcgg cgtaaaccgc atagtcatag tcgttcttgt ccggccggat gacgacgcca
    12001 aagtcaccga gcttattgcc aagacctagt ctgccgggaa ggacgaaaaa gtttaccgac
    12061 tccgcatcga cgtagcgacg cggatccttc gcgtccatgg aagtgtcctc gagagaggtc
    12121 gtggaaatgt agaatccctt cgccggatca gacttcgctt gaataaccgg gttaccgttc
    12181 ggtttgccgt tgtccgtcac gagggcccac cagttgcccg gcttgccggc attacccaaa
    12241 taatcaagtc ccgatatatt atccggatgg tatgcgtggt aaccaccatc cgcatcgatc
    12301 atcatcccag actcgtagaa gaaagcggag tcgtcgtcct gcagtgtgtt gatatcggag
    12361 tatccgaccc ttttaaggaa ttttaatttt gccataaatt gatgcgctgt tttgttcgtt
    12421 aggcgcaaga tcaaccggta ttccggtggg agacacgggt ggttcaggcc cacgatcaac
    12481 tcccatagcg acgttaagga tgccagtcac aggaatgcct ttagagaaga gcgaagccga
    12541 ctgccttgtt caaggcgtcg ctcgcgaccg cgagagcggc gttgatagcg tcttgcgcgg
    12601 cggccttgag tgtgacgtcg atcgcgagaa gggacgtcat ttcggagtcc tttatctgct
    12661 gaaacgcaaa ctttacatcg tcgtccgtga tattgttagc cacccgatct gcctcgagct
    12721 caaccatccc gtcggcaacc agtttcgcgc gtgcttccag gtaactctta agatctgcgg
    12781 cgtggccttt tgccgctccc gtcgcggccg cgagcatgtc ttggatcaaa tcggatgtgt
    12841 caatggacat gggttattgg tttgagttgg ttttcttgag ccctgcgctg aaaacggagc
    12901 tgcgtttctt tgcaatttga atttgttgca gatcaagcaa cgtggcacgc aacccagtcg
    12961 caagagagct gcgcggaggg ccgccgtgct gccggagatc ctggtagcgt tgctccagtt
    13021 cactaaacgc tttgacttcg tcctgattct tgtcgaaaag ggaggaccgc gctttcatcg
    13081 cgcgcaccgc accgatcgct tcgtcaaaga atttctccct gtcgctcgtc gataactggc
    13141 cggcatcggc ctgtgcgatg gcggtttcag tctttaccga aagatcagtc agcagatggt
    13201 cggttgtctc atcgtaaggg gcaaccaagg tgcaaccggc gaagaggacg gcggcgagaa
    13261 agagaatcgg cagtttcgct ttaatcagtc tgctcatagt tagaatttta gctggtcgag
    13321 accggcgctc tttgtctcaa caagcctttc gtagccgctc atggcgtggt cgccgacgtc
    13381 aagaccgacg tggccggcga tctcgaaaaa ctgctcgaag ctgtcgatcg tgcggctaag
    13441 gaaattcgac cattcgctgc tctggagttg cggattgccg ctgctgtcaa agtggatcat
    13501 ctggccgata tcggcgtaat cgaatcctcg aaacgggacg cgcggaacga gatctttatt
    13561 gtttacgaag cgaaaggtct tgcggctgag gaggtggtcg taattgccgc aaaaacgaaa
    13621 caaaccgacg cgtggctggc cgaaagtgta gatgccgtt
//
LOCUS       DATMNS010000049         8414 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2566337, whole genome shotgun sequence.
ACCESSION   DATMNS010000049 DATMNS010000000
VERSION     DATMNS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2566337"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1669)
                     /gene="gpmI"
                     /locus_tag="VNW28_01935"
     CDS             complement(134..1669)
                     /gene="gpmI"
                     /locus_tag="VNW28_01935"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HXA08710.1"
                     /translation="MAKPIVLVIRDGWGINPGGKAEREKNGDATLLAQTPFHDRLYRE
                     YPCATLSASGEDVGLPAGQMGNSEVGHLNLGAGRIVYQDLTRINKAIADGELKKNEVL
                     QEAFAKARSGRLHFIGLVSDGGVHSHYQHLLALAAAAKAAGVEQILVHAITDGRDASP
                     TGGVDYLTICGAQLARSGAQIITVVGRYFAMDRDRRWDRTKLAWDAIVLGRGEVCAEA
                     PSAAIRRHYATGKTDEFMPALIFAEPNEQRIRDGDTVIFFNFRADRARQLSQAFLLKD
                     FDGLDREVWPRVHYVTMTEYDATYDCPMVFGPQSLKNILDDVVSAAGKKQLRIAETEK
                     YPHVTYFFNGGVEKPAPGEDRKIIPSPKVATYDLQPEMSAGQVTDEVLSRVNQYDLII
                     LNYANPDMVGHTGVVAAGIKAVETIDTCIARLVPAVLSSGGKLLLTADHGNCEQMRNP
                     DGSPNTAHTTNLVHLIYVADDAKNFRLESGILSDIAPTLLFLLGLPKPAEMTGHCLLV
                     PVQ"
     gene            1763..3037
                     /locus_tag="VNW28_01940"
     CDS             1763..3037
                     /locus_tag="VNW28_01940"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HXA08711.1"
                     /translation="MKWRNQILALLCLTVFVALGVLYFRHWVVQKPFGIVLFIGEGLT
                     PDRVAATRLYAGGANTRLAIESLPAVAMLTNFSQDFAVPDDAAAATTLATGVKVNNRS
                     LGLGPDGEKLNSIIDLAREKGRAIGLVTNARLTDPTVAAFYGRGKGGVQEVARQLADE
                     KKIDIVLGGGGSDFLPEAKGGQRTDGRDLVLELRRNGFEIVRTKAELEGIPQWRRPKL
                     LGLFSDGEMAFATDIASGSDQPALADMTRRAIQLLQWNAGGYLLIVDAGLMRSAAREN
                     HGERTLAETVELDRAVAAARSYAGDNSTVLVAGNFGIGGLSLNGFPFRRDSGVALLGI
                     NSSGEPALTWATGPNGIVTRSAPPGSALEDPAPPGEGPPPASGQEPAAVYAPWARNTV
                     ADVLAAGSGPGTETLHGFLDNTIVFKIIAGQL"
     gene            complement(3123..4340)
                     /locus_tag="VNW28_01945"
     CDS             complement(3123..4340)
                     /locus_tag="VNW28_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA08712.1"
                     /translation="MRVDSTLKIAFRALQRNKLRSVLTALGIIIGVGAVIAMVSIGNG
                     AKAQVEQQIASLGENVILIFSGSVTSSGIRTGWGSAGTLKIEDAEAIQREVPGVIAVS
                     EEVFSTSQVSAGNQNWFTRIYGESPEYFDIRQWPLSDGANFTPQDVRSANKVCVIGST
                     TASQIFGAESPVGQVLRIKGVPFLITGVLTPKGLSPQGTDQDDIVIMPYTSAMKRVIG
                     GTTLRGINVQVASPNDLAPAQQQITELLRQRHNIRAGRDDDFTVRNQQEIAETATATS
                     RIMTLLLGAIASVSLVVGGIGIMNIMLVSVTERTREIGVRLAVGAHGRDILTQFLIEA
                     VTLSVIGGVIGILLGLGASRMLSVFAHWPTLISIPSIIAAFFVSAAVGIFFGFYPARE
                     AARLDPIEALRYE"
     gene            complement(4337..5086)
                     /locus_tag="VNW28_01950"
     CDS             complement(4337..5086)
                     /locus_tag="VNW28_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA08713.1"
                     /translation="MDGNATVVRLEDVHKTYHTGEVEVQAVRGVSLEIAHGEFVAFMG
                     ASGSGKSTLMNLLGCLDRPTKGRYLLDGFDVSGLDRDQLADIRNRKLGFVFQSFNLLA
                     RTSALENVELPLLYGEHRLTNAQLRAQAEKVLAAVGLTGREDHHPSQLSGGQQQRVAI
                     ARALINDPEVVLADEPTGNLDSRTGAEIMDIFQKLNERGITIVMVTHETDIAAYARRN
                     ITMRDGLVQTDRPVALRLRAEDAAKNLEPPA"
     gene            complement(5097..6263)
                     /locus_tag="VNW28_01955"
     CDS             complement(5097..6263)
                     /locus_tag="VNW28_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXA08714.1"
                     /translation="MKRITLIGGAVLLVLLALFAFRRCGGNGVAEYQTATVTRGAITQ
                     AVTATGTLNPVVNVQVGSQVSGNIQKLFVDFNSKVKAGEVVAQIDPVLFQAAVTQAEG
                     DLASAQAALELAKINAVRTQELFEKKNSAQADVDQAMANLHQAEANVKFKQGALDKAQ
                     ADLDHCTITSPVDGEVISRSVDVGQTVAASLSAPVIFTIANDLTKMQIDANVAEADVG
                     TVEGGQTVDFTVDAFPRRTFHGDVVQVRNAPISVQNVVTYDTVIGVNNADLKLKPGMT
                     ANVSIVGAQRDNALKISNAALRFRPPDATPPPSGSPGRRGKPGEHATERTVYVLRGSK
                     PNPVQIKTGISDGIYTEVLDGLQEGDRVVTAMTGRAASSAQPSNNPLAGGQRHF"
     gene            6338..7132
                     /locus_tag="VNW28_01960"
     CDS             6338..7132
                     /locus_tag="VNW28_01960"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HXA08715.1"
                     /translation="MRRYLEIYSIMLRNSLIRELSFKANFILWMIVEVLWFCGQILFF
                     SIIFGQVNQIGDWTKWEVVLLVGTHQIIAQLFQAFFFVNVANVPELVRTGKLDSLLVL
                     PIDSQFAVSTKQFGLDSVINALIGAAVVIFSLRQLHVVPSPLAVALYLVALGFGVAIH
                     YSIMLALAAVSFWIVRAQGLVYGYFNFLNIARYPDVIFPQLFRWVFGWIIPVIIVANI
                     PARLLIKSLGQPAWLMFHLIVAGTIAALLSRAFWRWALRHYSSASS"
     gene            complement(7211..7510)
                     /locus_tag="VNW28_01965"
     CDS             complement(7211..7510)
                     /locus_tag="VNW28_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08716.1"
                     /translation="MSVWLALPPAFDALPLADRTAEAVGLLDLSGFALCTGAGFFFAA
                     GVFFLVAFLGAAFFAGFLEAGFFAGFLGAGFFIERAIKVIVGAAFPLVRTKGRVN"
     gene            7527..8018
                     /locus_tag="VNW28_01970"
     CDS             7527..8018
                     /locus_tag="VNW28_01970"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HXA08717.1"
                     /translation="MKPKKERRLDPFTRGQKEKLLQLRDAMVDSMAGVAQDTLRARAE
                     GSEASAFGMHQADAGSDAYDRDFALSLLSQEQDALYEIDEALKRIDLGTYGKCEMSGK
                     PILRARLEAIPFARFTVECQSQLEKQSKASRVRQSVTSLFGLTDEEAREGEEEEPVAD
                     TKE"
     gene            8024..8197
                     /gene="rpmG"
                     /locus_tag="VNW28_01975"
     CDS             8024..8197
                     /gene="rpmG"
                     /locus_tag="VNW28_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXA08718.1"
                     /translation="MAQEIITLECTEAKAAGAPVSRYMTTRNKKSPRTPNRLEKKKYN
                     PFLRRHTLHRETK"
     gene            8200..>8414
                     /gene="rpsR"
                     /locus_tag="VNW28_01980"
     CDS             8200..>8414
                     /gene="rpsR"
                     /locus_tag="VNW28_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HXA08719.1"
                     /translation="MQPKTAKRRSAFRRANRTMPRRRIDIAAEAIDYKNPDLLRKFVT
                     ESGKILPRRVTGMPAHMHRKITREIKR"
BASE COUNT         1614 a         2681 c         2520 g         1599 t
ORIGIN      
        1 gcgtgattga gcgcgcccac atgctcgggt tcctcgatcc gaatctcccg ccgcgaaaac
       61 agggccgcga gccggcaaca ctggcgtcgt aattgtagcc gccgccctgt gggcggcgcg
      121 gcgcaggacg tcgctactgg accggcacca gcaggcaatg ccccgtcatc tccgccggct
      181 tgggcagccc aagcaggaaa agcaacgtcg gcgcgatgtc ggacaggatt ccgctctcca
      241 ggcgaaaatt tttcgcgtca tccgccacgt aaatgaggtg gaccaggttg gtggtgtggg
      301 cggtgttggg cgaaccgtcg ggattgcgca tctgctcaca atttccgtga tccgcggtca
      361 ggagcaattt tccgcctgac gacaagacag cgggaacgag ccgcgcgatg caggtgtcga
      421 tcgtctcgac cgctttgatt ccggcggcga cgaccccggt atgaccgacc atgtcgggat
      481 tcgcgtagtt caagatgatc aaatcgtatt ggttgacccg gctgagcacc tcgtcggtta
      541 cctgccccgc gctcatctcc ggctgcaaat cgtaggtcgc gaccttgggc gacggaataa
      601 tcttgcgatc ctcgcccggc gccggtttct cgaccccgcc gttaaagaaa taagtcacgt
      661 gcggatattt ctccgtttcg gcaattcgca gttgcttttt ccccgccgcg ctcaccacat
      721 cgtcgagaat attcttgagt gattgcggtc caaagaccat cgggcaatcg taggtcgcgt
      781 catattccgt catggtgacg taatgcacgc gcggccaaac ttcccggtcg agaccgtcga
      841 aatccttgag cagaaaagcc tgcgagagct gccgggcgcg gtcggcgcga aagttgaaga
      901 aaatcacggt gtcgccgtcg cggatacgct gctcgttagg ctcggcaaaa atcagggccg
      961 gcatgaattc gtccgtcttg ccggtcgcat aatggcgcct gatcgcggcc gacggcgcct
     1021 cggcgcagac ctcgccgcgc ccgagcacga tcgcgtccca ggccagcttc gtccggtccc
     1081 agcgccggtc gcgatccatc gcgaaatagc gcccgacaac ggtgatgatc tgcgccccgc
     1141 tgcgggccag ttgcgcgcca cagatcgtta ggtaatccac tccgcccgtc ggcgaagcgt
     1201 cgcgtccgtc ggtgatcgcg tgcaccaaga tctgctccac acccgccgcc ttcgcggccg
     1261 cggccagggc gaggagatgc tgataatgac tgtgaactcc gccgtcggaa accagcccga
     1321 tgaaatgcag ccggccgctc cgcgccttcg caaaagcttc ctgtagaact tcgttcttct
     1381 tcagttcgcc gtcggcgatc gccttgttga tccgggtcaa atcctggtaa acgatccggc
     1441 cggcgccgag attgagatgg ccgacctcgg aattgcccat ctgccccgcg ggcaaaccga
     1501 cgtcttcgcc gctcgcactc agcgtcgcgc agggatattc ccgatagagc cgatcgtgaa
     1561 acggcgtttg cgcgagcaac gtcgcgtcgc cgttcttttc ccgctccgct ttgcccccgg
     1621 gattgatccc ccacccgtcg cggatgacca aaacgatcgg cttcgccata gagcgcactt
     1681 tcccgacgcg cgctccgccc tcaagcgatt tcccgcggct gcgggattga caactacctt
     1741 cgcgcttcgc tctatccagt gaatgaaatg gcgtaaccag atcctggcgc ttctttgcct
     1801 gacggtattt gttgcgctcg gcgtccttta ttttcgtcat tgggtcgtgc aaaagccctt
     1861 cgggatcgtg ctcttcatcg gcgagggact gactcccgac cgcgtcgccg cgacgcggct
     1921 ctatgcgggt ggagcaaaca cgcgattggc gatcgaatcg ctcccagccg tggcgatgct
     1981 gaccaatttt tcgcaggact tcgccgtccc cgatgacgcc gccgccgcca ccacgctcgc
     2041 gaccggcgtg aaggtcaaca accgcagcct cggcctcggc ccggacggcg aaaagctgaa
     2101 cagcatcatc gacctcgccc gcgagaaggg gcgcgcgatt ggattggtca caaatgcgcg
     2161 cctaaccgat ccgacggtcg cggcgtttta cggccgaggc aaaggcggcg tccaggaggt
     2221 cgcgcgccaa ttggcggacg agaaaaaaat cgacatcgtg ctcggcggcg gcggctcgga
     2281 ttttctcccc gaagcgaagg gcgggcaacg cactgatggg cgcgacctcg tcctcgaact
     2341 gcgacgcaac ggtttcgaga tcgtccggac gaaagcggaa ttggaaggca ttccgcaatg
     2401 gcgacgaccg aagttgctcg gacttttcag cgacggcgaa atggctttcg ccaccgacat
     2461 cgcttcgggt agcgatcagc ccgctctcgc ggacatgaca cggcgcgcca tccaactcct
     2521 gcaatggaac gcgggtggtt acctgctgat cgtcgacgcc ggcctgatgc gcagcgccgc
     2581 gcgcgagaat cacggagagc gcaccctggc cgagaccgtc gagctggatc gtgcggtggc
     2641 cgcggcgcgc agctacgcgg gcgacaactc gaccgttctg gtcgcgggca atttcggcat
     2701 cggcggactt tcgcttaatg gtttcccttt ccggcgcgac agcggcgtcg cgctccttgg
     2761 catcaactcg tccggcgaac cggcccttac gtgggcaacg ggcccgaatg gaatcgttac
     2821 ccgaagtgca ccgccgggat ccgccctgga agatcccgcg cctccggggg aaggcccgcc
     2881 tccggccagc ggacaagaac cggccgcggt ctatgcccct tgggcgcgca acacggtcgc
     2941 cgacgttctc gccgccggca gcggacctgg aacggagaca ctgcacgggt tcctcgataa
     3001 cacgatcgtc ttcaaaatca tcgcgggaca gttgtagacg cttcgctctg cgacgcgcgg
     3061 gcaagggagg ttcgagcgaa cgagatcgac acccgcgcct cccacggggt aggcgtctgc
     3121 gactactcgt aacgcagcgc ctcgatcgga tcgagccgcg ctgcttcccg cgccgggtag
     3181 aatccaaaaa atatcccgac cgccgcactg acaaaaaacg ccgcgataat cgacggaatc
     3241 gagatgagcg tcggccagtg cgcgaagacg gagagcatcc gcgaggcgcc aagtccgagc
     3301 aggattccga tcacgccgcc aatcacgctc agggtcaccg cctcgatcaa aaactgggtg
     3361 agaatgtcgc ggccgtgcgc gccgaccgcg aggcgcacgc cgatctcgcg cgtccgctcg
     3421 gtgacgctca cgagcatgat gttcataatg ccgatcccgc cgacgacgag cgagacactg
     3481 gcgatcgcgc ctaacaacaa ggtcatgatc cgcgaagtcg cggtcgcggt ctcggcgatt
     3541 tcctgctggt tgcggacggt gaagtcgtcg tcgcgcccgg cgcgaatgtt gtgccgctgg
     3601 cggagcagct cggtgatctg ttgttgcgcc ggcgcgagat cgttaggcga agcgacctgg
     3661 acgttgatcc cgcgcagagt cgttccaccg atgacgcgtt tcattgcgct cgtgtagggc
     3721 atgatgacga tgtcgtcctg gtccgttccc tgggggctga gccctttcgg ggtgagcacg
     3781 ccggtgatga gaaacggcac ccctttgatc cgcaggacct ggccgacggg actttccgcg
     3841 ccgaaaatct ggctcgcggt cgtgcttccg atgacacaca ccttgttcgc actccgcaca
     3901 tcctgcggag tgaaattcgc cccatcgctc aagggccatt gccggatatc gaaatactcc
     3961 ggcgactcac cgtagatgcg ggtgaaccag ttctggttcc cggccgagac ctggctggtg
     4021 gaaaacactt cctcgctcac cgcgatgact cccggcactt cgcgctggat tgcctccgcg
     4081 tcttcgatct tgagcgtgcc ggcacttccc cagccggtgc gaattccgct cgaagtcacg
     4141 ctcccggaaa aaatcaggat cacattttcg ccgaggctgg cgatctgttg ttcgacctgc
     4201 gccttcgcgc cattaccaat gctgaccatc gcgatgacgg cgccgactcc gatgatgatg
     4261 ccgagcgcgg tcaggacgga gcgcagcttg tttcgctgca aggcgcggaa agcaatcttc
     4321 aaggtggagt cgactctcat gccggcggct ccagattctt cgctgcgtct tcggccctca
     4381 gccggagcgc taccgggcga tcggtttgca ccaggccatc gcgcatcgtg atgttgcgcc
     4441 gggcataggc cgcaatgtcg gtctcgtgcg tcaccatcac aatcgtgatc ccgcgctcgt
     4501 tgagtttttg aaaaatgtcc atgatctcgg caccggtgcg gctgtcgaga ttgccagtgg
     4561 gctcatcggc aaggacgacc tccggatcgt tgatcagggc gcgcgcgatc gcgacccgct
     4621 gctgctgacc gccggaaagc tggctcggat gatgatcttc acggccggtc aggccgaccg
     4681 cggcgagcac cttctcggcc tgcgcgcgca attgcgcgtt cgttaggcga tgctccccgt
     4741 agagcagggg cagctccaca ttctccagag ccgaggtgcg ggcgagcaga ttgaagctct
     4801 ggaaaacgaa accgagcttg cgattgcgaa tgtcggccag ctgatcgcga tcgagtcccg
     4861 aaacgtcgaa gccatcgaga agatagcggc ctttcgtcgg ccgatcgagg cagccgagca
     4921 gattcatgag cgtcgatttg cccgagccac tcgcgcccat gaaggcgaca aattcaccat
     4981 gcgcgatctc cagcgaaacg ccgcgcacgg cctgcacctc gacttcgcct gtgtggtagg
     5041 tcttgtgcac gtcctccagc ctaacgaccg tagcattacc gtccattgcg cttcggctaa
     5101 aagtggcgct gcccgccggc cagcggatta ttcgagggct gcgccgagga ggccgcccgg
     5161 ccggtcatcg ccgtcaccac gcgatcgcct tcctgcaaac catcgagcac ttcggtgtaa
     5221 atcccgtcgc tgattccggt cttgatctgc accgggttcg gcttggagcc gcgcaggacg
     5281 tagacggtcc gctcggtggc atgttcgccc ggcttgcccc ggcgtccggg cgaaccagac
     5341 ggcggcgggg tcgcgtccgg cggacggaag cggagcgccg cgttgctgat cttcaaagcg
     5401 ttatcccgtt gcgcgccgac gatcgagacg ttcgccgtca tgccgggctt gagcttgagg
     5461 tcggcgttgt tcacgccgat gacggtgtcg taagtgacga cattctgcac cgagatcggc
     5521 gcgttgcgca cctgcaccac gtcgccgtgg aaggttcgcc ggggaaatgc atcgactgta
     5581 aaatcgactg tctgccctcc ttccaccgtc ccgacatccg cctcggctac gttggcgtcg
     5641 atctgcattt tagtgagatc gttggcgatc gtaaaaataa ccggcgcgct caggctcgcc
     5701 gcgacggttt gcccgacatc gacactgcgc gaaatgacct cgccgtcgac gggcgacgtg
     5761 atcgtgcaat ggtcgagatc ggcctgcgct ttatcgagcg cgccttgttt gaatttcacg
     5821 ttggcctcag cctgatgcag attcgccatc gcctgatcga catcggcctg ggcggaattt
     5881 ttcttctcga acaattcctg ggtgcgcacg gcgttgattt tcgccagctc gagggcggct
     5941 tgtgcgctcg ccagatcgcc ctcggcctgg gtgacagccg cttggaacag gaccgggtcg
     6001 atctgggcca ccacctcgcc cgctttgacc ttggaattaa aatcgacaaa caacttttgg
     6061 atattgcccg agacctggct cccgacctgc acgttgacga ccggattgag cgtgccggtg
     6121 gccgtgaccg cttgcgtgat ggcgccccgc gtgacggtag cggtttgata ttcggccacg
     6181 ccattcccgc cgcaccggcg gaaggcaaag agcgccaaca ggacgagcaa gaccgcgccg
     6241 ccgataaggg tgattcgttt cattaaaaaa ggccgacgaa caggcgtcgg cagctattgt
     6301 ttagaccgct tggaccgaaa ttagttacac gataatcatg cgccgctatc tcgaaatcta
     6361 ctcgatcatg ctgcgcaact cgctcattcg cgagttgagt ttcaaggcca acttcatcct
     6421 ctggatgatc gtcgaggtgc tctggttttg cggccagatt ctcttcttca gcatcatttt
     6481 tggtcaggtc aatcaaatcg gtgactggac gaaatgggaa gtggtgctcc tcgtcggcac
     6541 acaccagatc atcgcgcagt tgttccaggc gtttttcttc gtcaatgtgg cgaacgttcc
     6601 cgagctggtg cggaccggga aactggattc gcttctcgtc ctgccgatcg acagccagtt
     6661 tgcggtctcg acgaagcaat tcggcctcga cagcgtgatc aacgcgctga tcggcgcggc
     6721 ggtggtgatt ttctccctgc ggcaattgca cgtcgtgccc agcccgctcg ccgtggccct
     6781 ctatctcgtg gcgctcgggt ttggggtcgc gatccactac tcgatcatgc tcgcgctcgc
     6841 cgcggtcagc ttctggatcg tccgcgcgca aggcctcgtt tacggctatt tcaattttct
     6901 caatatcgcc cgttatccgg acgtgatttt tccgcaactt ttccgctggg ttttcgggtg
     6961 gatcatcccg gtgatcattg tggcgaacat cccggcgcgc ttgctcatca agtcgctcgg
     7021 gcagcccgcc tggctgatgt ttcacctcat cgtcgccggg acgatcgcgg ccctgctcag
     7081 ccgcgccttt tggcggtggg cgctccggca ttactcgagc gccagctcct gagcggaagc
     7141 gcgccgctcc agagacaagc gcgctcccca aaatcgctcc aaaaccgctt gcgccaaaat
     7201 cgaaatgccc ttagttaacc ctccccttag tccggaccag aggaaaagct gcgccgacta
     7261 tcacttttat ggcccgttca atgaaaaaac ccgcacccaa aaagcccgca aaaaaaccag
     7321 cttccaagaa gccggcgaag aaggctgcgc ccaagaacgc caccaagaaa aaaaccccgg
     7381 ccgcgaagaa aaagccggcg cccgtgcaca aggcaaagcc gcttaagtcg agcaggccca
     7441 ctgcctctgc cgtccggtcc gccaatggca aggcgtcaaa ggcgggcggc aaagccaacc
     7501 aaacgctcac gattatcggt aatcacgtga agccgaaaaa ggaacgccgg ctcgatccct
     7561 ttacccgcgg gcaaaaggag aagcttcttc agctccgcga cgcgatggtg gattcgatgg
     7621 ccggtgtcgc gcaggacacc ttgcgcgcgc gggccgaggg aagcgaagct tcggctttcg
     7681 gtatgcacca ggcggatgcc gggagcgacg cttacgatcg cgatttcgcg ctcagtcttt
     7741 tgtcccagga acaggacgct ctttacgaga tcgacgaggc gctcaagcgc atcgatctcg
     7801 gcacctacgg gaaatgcgaa atgtcgggca agccgattct gcgcgcccgg ctcgaggcga
     7861 taccgtttgc ccgcttcacg gtggaatgcc agtcgcagct cgagaaacag agcaaggcgt
     7921 cgcgggttcg ccagtccgtc acctcgctct ttggcctgac cgacgaggaa gcgcgcgaag
     7981 gcgaagaaga agagccggtg gcagacacga aggaataacg aacatggctc aggaaatcat
     8041 caccctcgaa tgcaccgagg ccaaggcggc tggagcgcct gtctcgcgct acatgaccac
     8101 gcgcaacaag aaaagcccgc gcaccccgaa ccggctggag aagaaaaaat ataatccctt
     8161 cctcaggcgg cacacgctcc atcgggagac caagtaacca tgcaaccgaa aaccgccaaa
     8221 cgccgttccg cctttcgccg cgccaaccgc accatgcctc gtcgccggat cgacatcgcg
     8281 gccgaggcga tcgattacaa gaacccggat ttgcttcgca agttcgtcac cgagagcgga
     8341 aaaattcttc cgcgccgcgt gaccggaatg ccggcgcaca tgcaccgcaa gatcacgcgc
     8401 gaaatcaagc gcag
//
LOCUS       DATMNS010000050         6978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_419169, whole genome shotgun sequence.
ACCESSION   DATMNS010000050 DATMNS010000000
VERSION     DATMNS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419169"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="VNW28_01985"
     CDS             complement(<1..373)
                     /locus_tag="VNW28_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HXA08720.1"
                     /translation="MTTDHNTISAPDLTQRPPRSPRLRLGGYVLLPRMLDKGRAEIAG
                     KNGEYHYNCPLDQRIINFLGIDPAALRGQLAAGKGDGEILEWIQTNAKYQRTPWEIEQ
                     WSDYMQRRGPESDAETLQAFAE"
     gene            complement(415..756)
                     /gene="trxA"
                     /locus_tag="VNW28_01990"
     CDS             complement(415..756)
                     /gene="trxA"
                     /locus_tag="VNW28_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXA08721.1"
                     /translation="MNEPININEASFEKAVLHSPIPVLVDFWATWCGPCKMIAPLLDE
                     IAKESAGRFRIAKVNIDDDPALMQRFNVRAVPTLLFFSGGELRDQLMGGAPKKTILEK
                     LAALAGSPVSA"
     gene            complement(780..1844)
                     /locus_tag="VNW28_01995"
     CDS             complement(780..1844)
                     /locus_tag="VNW28_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXA08722.1"
                     /translation="MKALSQNQPRRWPALTPVWDSSDGSFGAHQEESLGEAGLLDVYS
                     QTIAAVVNRVAPSVVNIRVLNGERGPGGGSGFILARDGFILTNSHVVYGAHEIEVTLR
                     DARVYPAQLIGTDPETDLAVIRIDAPEVQHARLADSSQIRVGQIAVAVGSPYGFQQTV
                     TAGIVSALGRSMRSESGRLMDEIIQTDAALNPGNSGGPLLNSAGEVIGVNTAVILPAQ
                     GICFAIASNTAKFVAGWLIKDGRIRRSSIGVAGQNVPLHPRVVRFHKLPNERGVLVME
                     VEPGGPAAIAGLKTNDTIVGFKGQPVATVDDLHKQLVAAEIGVASPLMFLRGTEQLFC
                     MVVPRELPPLAARNRRRAPQ"
     gene            complement(1865..2599)
                     /locus_tag="VNW28_02000"
     CDS             complement(1865..2599)
                     /locus_tag="VNW28_02000"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXA08723.1"
                     /translation="MNNKKTALITGANKGIGLETARQLGEKDITVLMGARDAEKGKRA
                     ADKLRGEGIDAHAIVLDVSDPGSIQKAAAQVEKDFGRLDILVNNAGVMADDRGKKVSE
                     QTLAMWRETFETNVFGLIATTNAFLPLLRKSEAGRVVNLSSILGSTGFHAKPGSPIYE
                     SKDTPAYNVSKAAVNAYTVQLAYDLKDTKIKVNAAHPGWVKTDMGGPGAMMELEDGAK
                     TSVALATAGPDGPSGTFEHMGQALPW"
     gene            complement(2961..3572)
                     /locus_tag="VNW28_02005"
     CDS             complement(2961..3572)
                     /locus_tag="VNW28_02005"
                     /inference="COORDINATES: protein motif:HMM:NF028235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXA08724.1"
                     /translation="MPRKSDAKERLMNAAMDLIWENSYGSTSVDAICEKAGAKKGSFY
                     YFFESKSELAVAALEADWRRKKARMDEIFSAAVPPLERLSSYFKHVYEGQTKRHGECG
                     SVLGCPYCTLGCEIGTQDRAIGEQVHKILDRNARYFESAIRDAHAQGLIAAPDAKAKA
                     RMLVAFFQGCLAQARIENDVQPLRGLAAGALELLGASPARARK"
     gene            complement(3686..3817)
                     /locus_tag="VNW28_02010"
     CDS             complement(3686..3817)
                     /locus_tag="VNW28_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08725.1"
                     /translation="MLDPRIVAASTQGPAALEQGATGARDRMSGSSQGNRTETDTPN"
     gene            3786..5330
                     /locus_tag="VNW28_02015"
     CDS             3786..5330
                     /locus_tag="VNW28_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXA08726.1"
                     /translation="MLAATILGSSMAFIDGTVVNVALPALQRALNASVVDVQWVVEAY
                     SLLLAAFLLSGGSLGDQFGRRRIFLLGIMLFSAASAWCGFASSIGQLIAARAVQGFGA
                     ALLVPGSLAIISSAFPFEERGRAIGTWSGFSAITAAVGPVLGGWLIEHVSWRAVFFIN
                     IPLALVVIVISLWRVPESVREDKTILDWRGAILGVLGLGALVYGLIESSRLGFARPSV
                     VIALAGAVVLLTLFLIIEAKISQPMLPLALFRSRTFTGANLLTFLLYAALGGTLFFLP
                     LNLIQVQHYAPTAAGAALLPFILIIFVLSRWSGGLVERCGAKIPLVTGPLIAAAGFAL
                     FLRPEVGGSYWTTFFPAIVVLGTGMAVSVAPLTTTVMNSVPENRVGVASGINNAISRT
                     AGLLAIAVFGIVMLQTFNRRLDERLSGIQLSAPARATLGSQRDKLAGAELPAELNPDT
                     RTILTRAIEESFVGGFRVLMGLGALLAVGSAVTAAVLIRADSRKVCAPNEQRAGQAAA
                     KIRTRR"
     gene            5364..5771
                     /locus_tag="VNW28_02020"
     CDS             5364..5771
                     /locus_tag="VNW28_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXA08727.1"
                     /translation="MNTIRGIKLRWLHETLGDVTHQLARFQFTQLRSYRWHPAINVFQ
                     CDKGLRICVDLAGVDRSQVALTVEPQRVTIRGTREAPEPNDAEGRAVQMIAFEIDYGP
                     FERVIELPLPVDVAQVQAEQRNGLLWIELPFKR"
     gene            5768..>6978
                     /locus_tag="VNW28_02025"
     CDS             5768..>6978
                     /locus_tag="VNW28_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXA08728.1"
                     /translation="MSEQPFSALQGAGQIPPAISSPAPTGGSDAVPAIPGTLAILPVR
                     GFVVFPRTMSPINVQRAASIQLLNETLPQNKIIGLLAQRDESNDDPAVGDLYHVGTAA
                     IVLKLLRQTDDRVVIIVQALRRLAIRKILQTDPFLRAEIEPLALQPAPATKEFEAAFR
                     NLRDSAAKLLELTPDVPEQAHALVLGMEDAEELTDFLAPNLNIDVAQKQALLEENDLE
                     KRVRSVQASISSQLEIAQIQQKLQQDVQSQFSDAQRRAYLREQVKAIQKELGEGDSGA
                     EEQMAQLRKKLETAQPPEEVMKQAERELKRLDFIPQASPDYSVIVTYIETIAELPWSK
                     LSVDNLDLDQAQQILDRDHYDLEKVKRRLIEYLAVRKLNPQGHGAILCFLGPPGVGKT
                     SLGQSIADALGR"
BASE COUNT         1196 a         2184 c         2099 g         1499 t
ORIGIN      
        1 tctccgcgaa tgcctgcagc gtctcagcgt cgctctccgg tccgcgccgt tgcatgtaat
       61 cgctccattg ctcgatctcc cacggggtgc gctggtattt cgcgttggtc tgaatccatt
      121 cgagaatttc gccgtctcct ttcccggccg cgagttgtcc gcgcaacgct gcgggatcga
      181 tgccgagaaa attgatgatc cgctggtcga gcgggcagtt gtaatgatac tcgccgtttt
      241 ttccggcaat ctcggcgcgc cccttgtcga gcatgcgcgg cagaagcacg tagccgccga
      301 ggcgaagccg cggactgcgc ggtggacgtt gtgtgaggtc aggagcggag atggtgttgt
      361 ggtctgtagt catttgtttt tgggtaatga ttcgctttgg tcttaaaaga ttcgttaggc
      421 ggaaaccggg ctgcccgcca gggcggcaag tttttccagg attgttttct tgggcgcgcc
      481 acccatcagc tgatcgcgca attcgccacc gctgaagaag aggagcgtgg gcactgcccg
      541 gacattaaag cgctgcatca gcgccgggtc gtcgtcgatg ttcacctttg cgatccggaa
      601 acgtcctgca ctttccttcg caatttcatc cagcaatggc gcgatcattt tgcacggccc
      661 gcaccacgtg gcccagaaat cgaccaggac cgggatggga gaatgcagca ccgctttctc
      721 aaagctggct tcgttgatgt tgatgggttc gttcattttt tgttctttgg ttggtttgat
      781 tattgaggcg ctcgccgccg gttgcgcgcg gcgagcggcg gcagttcgcg cggcacgacc
      841 atgcagaaaa gctgctccgt tccgcgcaaa aacatgagcg gcgacgcgac accgatttcc
      901 gcggccacga gttgcttgtg caaatcgtcg accgtcgcga ccggctgacc tttgaaaccg
      961 acaatcgtgt cgttcgtctt gagccccgcg atcgcggccg gaccgcccgg ctccacttcc
     1021 atcacgagca cgccgcgctc gttaggcaat ttgtgaaagc ggaccacgcg gggatgcagc
     1081 ggcacgttct gaccggcgac gccgatcgag ctgcgccgga tgcgtccgtc cttgatcagc
     1141 cagccggcga cgaatttcgc ggtgttgctc gcgatcgcga aacaaatgcc ctgcgccggg
     1201 agaatgaccg cggtgttcac gccgattact tcgcccgcgc tgttgaggag cggaccgccg
     1261 gagttccccg gattgagcgc agcgtccgtc tgaatgattt cgtccatcaa gcggcccgat
     1321 tcggaacgca tcgagcgtcc gagcgcgctg acgatgccgg ccgtcaccgt ctgctggaac
     1381 ccgtagggcg agccgacggc caccgcgatc tgcccgactc ggatctgcga cgaatcggcc
     1441 agccgggcgt gttgcacttc cggcgcgtcg attcggatga cggccaggtc cgtttccgga
     1501 tcggtcccga ttagttgcgc cggatagaca cgggcatctc ggagcgtgac ctcgatctcg
     1561 tgggcgccgt agacgacatg gctgttcgtc aggataaagc cgtcgcgcgc gagaatgaag
     1621 ccggatcccc caccgggccc gcgttcgccg ttcaggacgc gaatgttgac cacgctggga
     1681 gcgacgcggt tgaccaccgc ggcgatcgtc tgcgaataaa catccagcag gccggcctcg
     1741 cctaacgact cttcctggtg cgcgccaaac gagccgtcgc tcgaatccca gacgggcgtc
     1801 aaagcgggcc atctcctggg ctgattttgc gagagtgctt tcattttcgg tggggaggcg
     1861 acgcttacca gggaagcgct tggcccatgt gctcgaaggt cccgctcggc ccgtccggcc
     1921 cggcagtcgc cagcgcgacg cttgtcttcg cgccatcctc gagttccatc atcgcgcccg
     1981 gtccgcccat gtcggtcttg acccagcccg gatgggccgc gttgaccttg atcttcgtgt
     2041 ccttcaagtc ataggcgagc tgcacggtgt aggcgttgac cgcggctttc gaaacgttgt
     2101 aggccggcgt atccttgctc tcgtaaatcg gcgagcccgg cttggcgtga aagccggttg
     2161 atccgaggat gctcgagaga ttgacgacgc ggcctgcttc gcttttgcgc agcaggggca
     2221 aaaaggcgtt cgtcgtcgcg atcaatccaa aaacgttggt ctcgaaagtt tctcgccaca
     2281 tcgcgagcgt ttgctcgctt accttcttcc ccctgtcatc cgccatcacc cccgcgttgt
     2341 ttacgaggat atcgagacgg ccgaagtctt tttcaacctg ggccgcggct ttctggatgc
     2401 tcccggggtc gctcacatcg aggacgatcg cgtgcgcgtc gattccctct ccgcgcagtt
     2461 tgtccgcggc gcgctttccc ttctccgcat cgcgcgctcc catgagcacc gtgatgtctt
     2521 tctccccgag ttggcgggcg gtctccagtc cgatgccctt gttcgcgccg gtgatgagtg
     2581 cagttttttt gttattcatg ctggttattc gtttctcggt ggctgctcag actgagggcc
     2641 taccttcctg cacgcgcagg tgcgcgagcg cggggaaaaa aagcgacgga aacggggtgg
     2701 cccaggccgc tttttccgct tcgcggaccg cacgcgcaat ccactcccgt tcggcgggaa
     2761 acgcttcttc gtagtgcgcc tgaatcgcgc tgcggagacg ggttattccg tctctcaact
     2821 tctggcaata atccgccgga acgttttcgg gcgggcggca gtcaaccgct tccgggagaa
     2881 ttcctttcgc attgagggag tttacttgac tggtcatcta tagtgccttt ctagttttag
     2941 ttttatgaaa tttgcatcct ttatttcctc gcgcgtgccg gcgacgcccc gagcaactcc
     3001 agcgctcccg ccgcgaggcc ccggagcggt tgcacgtcgt tttcaattcg cgcctgcgcc
     3061 aggcagcctt gaaaaaatgc caccagcatg cgcgcttttg ctttcgcatc cggcgcggcg
     3121 atcaggcctt gcgcatgcgc gtcgcgaatc gccgactcaa agtagcgcgc gttgcgatcg
     3181 agaattttgt gcacctgttc gccaatcgca cgatcctgcg ttccgatttc gcacccgagc
     3241 gtgcaatagg gacagcccag gaccgaaccg cactcgccgt gtcgtttcgt ctgcccttcg
     3301 taaacgtgtt tgaaataact gctcagacgt tccaaaggcg gcaccgcggc cgaaaaaatc
     3361 tcgtccatcc gcgctttctt tcgtcgccaa tccgcctcca gggccgcgac cgccagctcg
     3421 gacttggact cgaagaaata ataaaagctg ccctttttcg cgcccgcctt ctcgcagatg
     3481 gcgtcgaccg aagtggatcc gtagctgttt tcccagatca gatccatcgc cgcattcatc
     3541 aggcgctctt tggcgtcgct tttcctcggc atgacgcaaa atgcatatag ttgaccaggt
     3601 agtcaaatgc ttttttggcc caatttggac gcgcttaagc gcgccccgcc ggagggacat
     3661 gcttccgcat gtccacgctc gtgctctagt taggcgtgtc cgtttccgtg cgattgcctt
     3721 gcgacgagcc gctcatccga tcgcgtgcac ccgtcgcccc ctgctccaaa gcggccgggc
     3781 cctgggtgct cgccgcgacg atcctgggat cgagcatggc gtttatcgac ggcaccgtcg
     3841 tcaatgtcgc gttgcccgcg ttgcagcgag cactgaacgc cagtgtcgtt gacgtgcagt
     3901 gggtcgtcga ggcctattcg ctcctgctcg ccgcattcct cctctccggc ggctcgttag
     3961 gcgatcagtt cggacggcgg cggatttttt tgctcggcat catgcttttc tccgccgcct
     4021 cggcctggtg cggcttcgct tcgtcgatcg gtcagttgat cgcggctcgc gcggtgcagg
     4081 ggttcggcgc cgcgttgctc gtccccggaa gcctggccat catcagtagc gcgttcccgt
     4141 tcgaggagcg cggtcgcgcc atcgggacgt ggtcgggttt tagcgcgatc accgcagccg
     4201 ttggtccggt gctgggcggc tggctgatcg aacacgtctc ctggcgcgcg gtctttttta
     4261 tcaacatccc gctcgcgctc gtcgtgatcg tcatctcgct ctggcgcgtc ccggagagtg
     4321 tgcgcgagga taaaaccatc ctggactggc ggggcgcaat tcttggagtt ctcggcctgg
     4381 gggctttggt ttacgggctg attgaatcat cgcgactcgg cttcgcgcgc ccgtcagtcg
     4441 tgattgcgct cgccggcgcc gttgttctcc tcacgctttt cctgatcatc gaggcgaaaa
     4501 tttcgcagcc gatgttgccg ctcgcgctct ttcgctcccg cacctttacc ggcgcgaacc
     4561 tgctcacctt tctcctttac gcggcgctcg gcgggaccct cttttttctc ccgttgaatt
     4621 tgattcaggt ccagcattac gctccgactg cggcaggcgc ggccctcctt ccattcatcc
     4681 tgatcatctt cgtcctttca cgttggtcgg gcggcctggt cgaaaggtgc ggcgcaaaaa
     4741 ttccactggt gacgggccca ttgatcgccg ccgcgggctt cgctctcttt ctgcggcctg
     4801 aggtcggcgg cagttactgg accacttttt tcccggccat cgtcgttctc gggacgggaa
     4861 tggcggtgag cgtcgcgccc ctcacgacca cggtgatgaa ctccgtcccc gaaaatcgcg
     4921 tcggtgtcgc ctcgggaatc aataacgcga tttcgcgcac cgcggggttg ctcgcgatcg
     4981 cggtcttcgg catcgtgatg ctgcagacct ttaatcggcg actcgacgag cgcttgtcgg
     5041 gaattcagct atcagcaccg gcgcgcgcga cgctcggcag ccagcgggac aaactcgccg
     5101 gcgcggagct gccggcagaa ttgaatccgg atacgcggac gatcctcacg cgcgcgatcg
     5161 aagaatcctt cgtcggcggt tttcgcgttc tcatggggct gggcgctctc ctcgccgtgg
     5221 gaagcgcggt caccgccgcc gttctcattc gcgcggactc ccgaaaggtc tgcgcgccta
     5281 acgagcagcg cgcgggacag gccgcggcaa aaatccgcac gcgcaggtga cgcctcgcac
     5341 acgtaggtta gattgcagga cgcatgaaca cgatccgcgg aattaaactg cgctggctcc
     5401 acgagacgct cggcgacgtg acccaccaac tcgcccggtt ccagtttacc cagcttaggt
     5461 cgtatcgctg gcacccggcg atcaatgttt tccagtgcga caagggactg cgcatttgcg
     5521 tagatctcgc cggagtcgat cgctcgcagg ttgcactcac cgtcgagccg cagcgggtca
     5581 cgattcgcgg cacgcgcgag gcgccggagc cgaacgacgc cgaggggcgc gccgtgcaaa
     5641 tgatcgcttt cgaaattgat tatgggccgt tcgagcgcgt catcgagttg cctttgccag
     5701 tcgacgtcgc tcaagtgcaa gccgagcagc gcaacggatt actctggatc gagctgccct
     5761 tcaaaagatg agcgaacagc ctttcagcgc cttgcaaggc gcgggtcaaa ttcccccggc
     5821 gatcagcagc cccgcgccga ccggcggatc cgacgccgtg ccggcaattc caggcacgct
     5881 cgcgattctt cccgtgcgcg gctttgtcgt tttcccgcgg accatgtccc cgattaatgt
     5941 ccagcgcgcg gcgtcgatcc agttgctcaa cgagacgttg ccgcagaaca aaatcatcgg
     6001 cctgctcgcg caacgcgacg agagcaatga cgacccggca gtgggagacc tttaccatgt
     6061 cggcaccgcg gcgatcgtgc tgaaattatt gcgccagacg gatgatcggg tcgtcatcat
     6121 cgtgcaggcg ttgcggcgtc ttgcgatccg caaaatcctg caaacggatc cgttcctgcg
     6181 cgcggaaatt gagccgcttg ccctgcagcc tgcgccggcg acgaaggaat tcgaagccgc
     6241 ttttcgcaac ttgcgcgatt cggctgcgaa gctgctcgag ttgaccccgg acgtacccga
     6301 gcaggcgcac gcgctcgtcc tcgggatgga ggacgccgaa gagttgaccg atttcctggc
     6361 gccgaatctg aacatcgatg tcgcgcaaaa acaggcgctg ctcgaagaaa acgacctcga
     6421 gaaacgagtg cgcagcgtgc aggcgagcat ctcttcccag ctcgagatcg cgcagatcca
     6481 acagaagttg cagcaggatg tgcagtcaca attctccgac gcgcagcgcc gggcctactt
     6541 gcgtgagcag gtaaaggcca tccagaagga actgggcgaa ggcgattccg gcgcggaaga
     6601 gcagatggcg caattgcgca aaaaactgga gaccgcgcag ccgccggaag aggtgatgaa
     6661 acaggccgag cgggaattga agcggctcga tttcattccg caggcgagcc cggattattc
     6721 ggtcatcgtc acctatatcg aaacgatcgc ggaattgccc tggtcgaaac tgagtgtcga
     6781 caatctcgat ctcgaccagg cgcaacagat tctcgatcgc gatcattacg acctcgaaaa
     6841 agtgaagcgg cggctgatcg aatatctcgc cgtgcgcaaa ctcaatccgc aagggcacgg
     6901 cgcgattctt tgtttcctcg gcccgcccgg cgtgggcaaa acgagcctcg gacaatcgat
     6961 cgccgacgcg ctcgggcg
//
LOCUS       DATMNS010000051         2381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2569470, whole genome shotgun sequence.
ACCESSION   DATMNS010000051 DATMNS010000000
VERSION     DATMNS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2569470"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="VNW28_02030"
     CDS             <1..306
                     /locus_tag="VNW28_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08729.1"
                     /translation="VEEKILAALGLSSEDARTIAVASVTDAQKGEALVLLSTIEIDPG
                     QLRTQLRDAGVPNLWIPKTIRRVEQIPLLATGKLDLRRCSEIAATAEAATVNEKSPA"
     gene            complement(284..1003)
                     /locus_tag="VNW28_02035"
     CDS             complement(284..1003)
                     /locus_tag="VNW28_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08730.1"
                     /translation="MKKSLIVSRKQTLWAQAALTVLLALALTGAFWFATLPVALSDLI
                     TPTEMIAAGLPPGAVIKTADKPQFLTAVCAAIKQHRKSAAAITGTAVRAHHEYGGDIV
                     ATAVRCSSGERGECELTGAIVAAAISAWPDSAPALDDAAVAVAPDCADAIQSATANDG
                     REVLPSGEPRDNLGPRGLIKHQSSMRSEAEVLTNSQEPTVLICDNDRQRAIRAGQVNH
                     YLNSHPGSFVGNCMITPATSR"
     gene            complement(1147..>2381)
                     /locus_tag="VNW28_02040"
     CDS             complement(1147..>2381)
                     /locus_tag="VNW28_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXA08731.1"
                     /translation="PGIQMFPIMPEALPGSDNFPVSFVIASTASQERILELAKQIFLK
                     AMQAHVFQFGDIDTKIDQPQAQIVFDHDKVSAMGLDMQKVGADLSASIGGNFVNRFNM
                     DGLSYKVIPQIKRIDRLNPEQLQNIYVTGPNNQLVPLSTIASIKHATVARSLNRMQQL
                     NAVTISGVPAGSVDAALKFLEDEAHKVLPAGYVVDYTGGSRQLRIEGNNFGAVFGLAV
                     VLIFLVLAAQFNSFRDPLIILCGSVPLALFGAGIFMFLKMPFGKFPTDGWTTSFNIYS
                     QVGLVTLVGLVSKNGILIVQFANELQRQGLAKLAAVAQAARIRLRPIMMTSVATVAGH
                     FPLTLVTGAGAAARNSIGLVLVGGMTIGTIFTLFIVPSLYMLIAKEHHGGSLGGGERE
                     IEPEAAGEPELMPEFADA"
BASE COUNT          502 a          697 c          725 g          457 t
ORIGIN      
        1 gtcgaggaaa aaattctcgc cgcgctcggc ctcagcagtg aggacgcccg cacgatcgcg
       61 gtcgcgtcgg tcaccgacgc ccagaagggc gaagcgctcg tcctcttgag cacgatcgaa
      121 atcgatccgg ggcagttgcg cacgcaactg cgcgacgccg gagtgcctaa tctctggatc
      181 ccgaagacga ttcgacgagt ggaacaaatt ccgctcctgg cgaccggcaa gctcgacctg
      241 cgaagatgca gtgagatcgc tgcgaccgcg gaggcggcga ccgttaacga gaagtcgccg
      301 gcgtgatcat gcagttgccg acgaaggatc cgggatgact attgaggtaa tggttgacct
      361 gacccgcccg tatcgcgcgc tgccggtcgt tgtcgcaaat caggacagtt ggttcctggg
      421 aattggttaa aacctccgcc tccgatcgca tcgaagactg gtgtttgatc aggccgcgag
      481 ggccgaggtt gtcccggggc tcgccggacg gcaaaacttc tcttccgtcg ttcgcggtgg
      541 cactttgaat cgcgtcagca cagtctggcg cgacggcgac cgccgcatcg tcgagcgccg
      601 gggcggagtc gggccaggca gagatggccg cggcaacgat ggctccggtg agctcgcatt
      661 cgcccctttc gccgctgcta cagcgcaccg ccgtcgccac aatatcaccc ccatattcgt
      721 gatgcgccct aaccgccgtc cccgtaatcg cggctgccga cttgcggtgt tgcttgatcg
      781 cggcgcagac cgccgtcaaa aattgcggct tgtctgccgt cttgatcacc gcgccaggcg
      841 gcaaaccggc cgcgatcatt tccgtcggcg tgatcaagtc agatagcgcc acggggagag
      901 tggcgaacca gaaagcacca gtcagcgcga gagccagcag cacggtcaga gcggcctggg
      961 cccagagcgt ttgtttccgc gaaacgatca gcgatttctt catatgattt agccagcgca
     1021 cgcaaatgta tgaatcattc gccaggaatg ctcaagctca agagtgatgc gttgccgttt
     1081 tgtcaggcac tcggtcgacg ctcgcttgaa gggcgccccg gccttaacca aaccagcctg
     1141 cggtcgtcag gcgtcggcaa actcgggcat caactccggt tcgccggcgg cctccggttc
     1201 gatttcgcgc tcgcctcctc ccagagagcc tccgtgatgc tccttggcga tcagcatgta
     1261 gagcgagggg acaatgaaaa gggtgaagat ggtgccgatt gtcattccac cgacaaggac
     1321 aagaccgatc gagtttcgcg cggcggcgcc cgcgccggta acgagggtaa gcgggaagtg
     1381 accagcaacg gtcgccacgc tcgtcatcat gatgggtcgc aaacggatgc gcgcggcttg
     1441 cgcgacggcg gcgagtttcg ccagaccctg tcgttgcagc tcattcgcga attgcacaat
     1501 cagaattccg tttttcgaaa caagaccgac gagggtaacg agaccgactt gcgagtagat
     1561 gttgaacgaa gtcgtccaac cgtccgtggg aaatttcccg aagggcattt tcagaaacat
     1621 gaagattcca gcgccgaaga gggcgagcgg gacggagcca cacagaataa tcagcggatc
     1681 gcggaaactg ttgaattgcg ccgccagaac aagaaagatc agaacgacgg ccagcccgaa
     1741 gactgcgcca aagttgttgc cttcaattct gagttggcgc gagccgccgg tgtaatcgac
     1801 cacatagccg gccggcagaa ccttgtgggc ttcgtcctcg aggaatttta gcgcggcatc
     1861 gaccgaacca gccggcacgc cgctgatcgt gaccgcgttg agctgctgca tgcgattgag
     1921 cgaacgcgcg acggttgcgt gtttgatcga tgcgatcgtg ctcaagggta cgagctgatt
     1981 atttggcccg gtgacgtaaa tgttctgcaa ctgttccggg ttcaaccggt cgatccgctt
     2041 gatttgcggg atcaccttgt agctcaggcc atccatgttg aaccggttga cgaaatttcc
     2101 gccgatgctg gcggagagat cggcacccac tttctgcata tcgagcccca tggccgagac
     2161 cttgtcgtga tcgaacacga tctgcgcctg cggctgatcg atcttggtat cgatgtcacc
     2221 aaactgaaag acgtgcgctt gcatcgcctt cagaaaaatc tgcttcgcca gctccaaaat
     2281 ccgttcctgg ctggcggtgg aagcgatgac gaagctgacc ggaaaattat cactccccgg
     2341 aagcgcttcg ggcatgatcg ggaacatctg gatcccggga a
//
LOCUS       DATMNS010000052         3722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_69136, whole genome shotgun sequence.
ACCESSION   DATMNS010000052 DATMNS010000000
VERSION     DATMNS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69136"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VNW28_02045"
     CDS             <1..516
                     /locus_tag="VNW28_02045"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXA08732.1"
                     /translation="PEQRMALLEDGAPLATYPVSTSKFGLGDLPGSRGTPLGTLEVAQ
                     KIGGGAPSGAVFKDRCQTGEIVPPDAPGRDPIVTRILWLRGAEPQNARAYSRYIYIHG
                     TPEERNIGLPVSYGCIRMRSCDVIALFNVVGTGAQVRIADAPLAALVPGLPPPTVRVA
                     TWRATSDSEIR"
     gene            complement(538..1551)
                     /gene="gmd"
                     /locus_tag="VNW28_02050"
     CDS             complement(538..1551)
                     /gene="gmd"
                     /locus_tag="VNW28_02050"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXA08733.1"
                     /translation="MKKALITGITGQDGSYLADLLLQKGYEVHGIIRRASTFNTARID
                     HLYADPHINGVRLFLHYGDLSDSVSLVKLIYELKPDEIYHLGAQSHVRVSFDIPEYTA
                     DVSGVGTIRILEAIREAGVRSRFYQASSSEMFGKVQAVPQTETTPFWPRSPYGVAKVF
                     AYWATVNYRESYGLCASNGILFNHESPRRGETFVTRKITRAVAAIKHGLQHELFLGNL
                     EAKRDWGYAPEYVEGMWRILQHDAGDDFVLATNETHTIREFLEIAFGRADLDWKQYVK
                     HDQRYERPAEVDLLIGDPAKAKRVLDWEAKVRFRELVELMVDADLKLLAQPTAREQLG
                     QLP"
     gene            complement(1598..2341)
                     /gene="rph"
                     /locus_tag="VNW28_02055"
     CDS             complement(1598..2341)
                     /gene="rph"
                     /locus_tag="VNW28_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXA08734.1"
                     /translation="MNRLDDRRSDQLRPVNFEVGIAPHASGSVLIAMGNTRVLCGVMI
                     EESVPRWMKDQRVSGGWLTAEYSMLPYSTLTRKPRDISKGRLDGRSSEIQRLIGRAMR
                     AALDLEKLGPRTIWVDCDVLQADGGTRTAAITGGSLAVSLACRRLVEEKKIPESPIKK
                     LVAAVSAGVIGDVALLDLDYEEDKAATVDLNLVATEDGALVEVQGAGEEATFSHAQLL
                     EMLSLGGKGIAELISAQRSVLASSAAAAG"
     gene            2882..>3722
                     /locus_tag="VNW28_02060"
     rRNA            2882..>3722
                     /locus_tag="VNW28_02060"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          782 a         1084 c         1105 g          751 t
ORIGIN      
        1 ccggaacaac gcatggcgct gctcgaagac ggcgctcccc tcgccaccta tccggtttcg
       61 acgtcgaagt ttggactcgg cgaccttccc gggagccgcg gaacgccgct cggcacgctc
      121 gaggtggcgc aaaaaattgg cggcggcgcg cccagcggcg ccgtcttcaa ggatcggtgc
      181 cagaccggcg aaattgtgcc gccggacgcg ccgggccgcg atccgatcgt gacgagaatt
      241 ctttggttgc gcggcgcaga gccgcagaat gcgcgggcct attcgcgcta catctacatt
      301 cacggcacgc cggaggagcg aaacattggc ctgcccgtca gctacggttg catccgcatg
      361 cgctcgtgcg acgtgatcgc gcttttcaac gtcgtcggga ccggcgccca ggtcagaatc
      421 gcggacgcgc cgctcgccgc gctcgtcccg ggattgcccc cgcccacggt ccgcgtcgcg
      481 acttggcggg cgacctcgga ttcggagatt cgttagggcc gaaagcccgc cagccgctca
      541 tgggagttgc ccgagctgct cgcgcgcggt cggttgcgcc agcagcttca ggtcggcgtc
      601 gaccatcagt tcgaccagtt cgcggaaacg gactttcgct tcccaatcga gaaccctttt
      661 cgccttggcg ggatcgccga tcaaaaggtc cacttccgcc gggcgctcgt agcgttgatc
      721 gtgctttaca tattgtttcc aatcgagatc ggcccggccg aaagcgatct cgagaaattc
      781 acggatggtg tgcgtctcgt tggtcgcgag gacgaaatcg tcgccggcgt cgtgctgcag
      841 gatgcgccac atgccctcga catattcggg ggcgtaaccc cagtcgcgtt tcgcctcgag
      901 gttgccgagg aaaagctcgt gctggagccc gtgcttaatc gcggcgacgg cgcgggtgat
      961 cttgcgcgtg acaaacgttt cgccgcggcg cggactctcg tggttgaaca aaatcccgtt
     1021 gctcgcgcag agcccgtaac tttcgcggta gttcaccgtc gcccagtagg cgaagacctt
     1081 ggccacacca tacggactgc gcggccaaaa cggcgtcgtc tccgtctgcg gcaccgcctg
     1141 caccttgccg aacatttcgc tgctcgaggc ctggtaaaaa cgggagcgca ccccggcctc
     1201 gcggatcgcc tcgaggatgc ggatcgtccc gacgccgctg acgtcggccg tatattccgg
     1261 aatgtcaaaa ctgacccgca cgtggctttg cgcgccgagg tggtagatct cgtcgggctt
     1321 caattcgtag atcagtttca cgaggctcac cgagtcgctc aggtcgccgt aatgcagaaa
     1381 gagccggacg ccgttgatgt gcggatcggc gtaaagatga tcgatgcgcg cagtgttgaa
     1441 ggtgctggcc cgccggatga tgccgtggac ttcgtaccct ttctggagga gcagatccgc
     1501 gaggtagctg ccgtcctgcc cggtaatccc ggtgatcaat gctttcttca tcgcgcggtc
     1561 tccgctctaa cgcatcccga ggggcgcggc aaacatttta cccggcggcg gcggccgagg
     1621 atgcgagcac ggaacgctgc gccgagatca gctcggcgat gcctttcccg cctaacgaca
     1681 acatttccag gagctgcgca tgagaaaatg tcgcctcctc gcccgcaccc tgcacttcga
     1741 ccagcgcgcc gtcttccgtg gcgacgagat taaggtccac cgtggctgcc ttgtcttctt
     1801 cgtaatcgag atcgagcagc gccacgtcgc caatcacacc cgcgctgacc gccgcgacca
     1861 gtttcttgat cggcgattcc ggaatctttt tttcctctac cagccgccgg caggcgaggg
     1921 aaacggcgag gctcccgccc gtaatcgcgg ccgtgcgcgt tccgccatcg gcctgcagga
     1981 catcgcaatc gacccagatg gtgcggggcc ccaatttctc caaatcgagc gccgcgcgca
     2041 tcgcgcggcc gatcagccgc tgaatctcgc tgctccgtcc atcgagccgc cctttggaaa
     2101 tatcacgcgg ttttcgcgtc agtgtcgaat agggcagcat cgaatattca gccgtcagcc
     2161 agccgccgct cacccgctgg tctttcatcc agcgtgggac cgattcctcg atcattacgc
     2221 cgcaaagaac ccgcgtattt cccatcgcaa tcaagaccga accactggca tgcggcgcaa
     2281 ttccaacttc gaaatttacc gggcgcaatt gatcgctgcg ccggtcgtcg aggcgattca
     2341 ttccgcccaa attagatgaa ctcccgagcc gcgctatgct taaaatgagc cgtcgtactt
     2401 catttgcgga ttgtcctggc gctcgccgtc ccaaaaatga gccgacatgc ccattccgac
     2461 agttcccggc aaaaattttg cgaaaagttt cttttcccgg ttgacgatga ccgcgcgcgg
     2521 gctattttcg caatcccgcc ggaagcgggc cccgaaactt ttcggggtgg ttctcgcaaa
     2581 ttttgggact gcaaattgga actcaatttt gtcccggcat gatgcacggc agcccgcccc
     2641 ggcgggcggg ccctgaatta tcgtcggcgc caggattggg tttttttgac gcccggaaga
     2701 tgcgcgagtt taccccgaag aaattcggga ctggttcttt gaaatctaca tacagggtag
     2761 taaaaacaac tttttagagc tgagtctgag aaatacgacc ggaaggtaag atgtccggtc
     2821 cgagagtttc agttatcaag tttgagcgtc gaatcggcaa cgatttggcg ttctacagat
     2881 tgatcaagct acaaagagca cataatggat gccttggtgc caataggcga tgaaggacgt
     2941 ggctaactgc gataagcctc ggagagcggt aaacacgctt tgacccgggg atttccgaat
     3001 gggataacct gagtgggata atacctgctc gagctcatgt gaatctatag catgagcatc
     3061 gcgatacccg gtgaagtgaa acatctcagt aaccggagga aaagaaagcg aaagcgattc
     3121 cgtcagtagt ggcgagcgaa agcggaagag accaaaccag cagcttgctg ttggggttgt
     3181 aggagcccga tgtggtagtg caacgattag atgaagcgaa tggaaaatcg ctcccgagag
     3241 ggtgacaggc ccgtaatcga aaatccgcgc acgcctaggg atttcctgag taatacgatg
     3301 cacgtgaaac ttcgtatgaa tccacgccga ccacggcgta agtctaaata ctaattggcg
     3361 accgatagtg aacaagtacc gcgagggaaa ggtgaaaaga accgctgtga gcggagtgaa
     3421 atagtacctg aaattatgtg cttacaaggt gtcaaagctg gcgcaagctg gtgatggcgt
     3481 gccttttgct taatgagtct gcgagttagt gtcagtggcg agcctaaggc gttctgcgcc
     3541 ggaggcgaag cgaaagcgag tccgaaatgg gcgcccatag tcgctggcac tagacccgaa
     3601 gccgaggtga tctacccatg agcaggttga agattgctta accgcaattg aaggaccgaa
     3661 cccgtgaagg ttgaaaactt ctgggatgac ttgtgggtag gagcgaaaga ctaatcaaac
     3721 cc
//
LOCUS       DATMNS010000053         3712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3255891, whole genome shotgun sequence.
ACCESSION   DATMNS010000053 DATMNS010000000
VERSION     DATMNS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3255891"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VNW28_02065"
     CDS             complement(<1..306)
                     /locus_tag="VNW28_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HXA08735.1"
                     /translation="MDEFTQALNATIAALDEMERATDRPLRASRNALDRLCHLPPLAT
                     DKAQRLEPIRQRVSVCLRCPHLASARTQTVFGVGNPNAELMFIGEAPGADEDRQGEPF
                     "
     gene            366..1346
                     /gene="queA"
                     /locus_tag="VNW28_02070"
     CDS             366..1346
                     /gene="queA"
                     /locus_tag="VNW28_02070"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXA08736.1"
                     /translation="MSGRVDEYDFALPEELIAQRPLPRREDARMMVLDRARGTIAHRR
                     FQELGEFVRPGDLLVLNDTRVLPARRFSDDGAIEFLLLEQVDPQIWKCLVKPGRKMRL
                     GATAVLDGVTGRVEKILDEGERLVRFEQAIDPYHGGDVPLPPYLQRAADAEDAARYQT
                     VFAKTPGAVAAPTAGLHFTQEILAKLPHTFVTLHIGTGTFRPVQSATIGGHRMHAERF
                     TITEEAAAAINAAERVLAVGTTSVRVLEAAVRRKTRLIAQEGATDIFIHPPHTFRAVD
                     RLLTNFHLPRSTLLMLVAAFAGREFILRAYAEAVGERYRFYSYGDCMLIL"
     gene            1494..1889
                     /locus_tag="VNW28_02075"
     CDS             1494..1889
                     /locus_tag="VNW28_02075"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HXA08737.1"
                     /translation="MESSETSDKPKGELVTRTVAMPKDTNPNGDIFGGWLMSQMDLGS
                     GILASKTARTRVVTVALDGMSFLEPVRVGDTVACYAWVESIGRTSMKIPVEVWVQRYM
                     RTEQTRVTHAVFTYVAVDQAGKPIPVPRD"
     gene            complement(1923..2186)
                     /locus_tag="VNW28_02080"
     CDS             complement(1923..2186)
                     /locus_tag="VNW28_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08738.1"
                     /translation="MKEKLRAAALLLVGITLLSGCSMLTANGRREHAYARYVQKSSLG
                     RVKQQKKLRPQKVKIPETQPSEPVIATEASGSGPQSVSDGSGH"
     gene            complement(2183..3220)
                     /locus_tag="VNW28_02085"
     CDS             complement(2183..3220)
                     /locus_tag="VNW28_02085"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXA08739.1"
                     /translation="MLQRDFFAFCTSLHLIELKAIGILSKVRHFAENDLVYSSGEVGD
                     ELFIINRGAVEILPHNARPGSPTTVLSRGDIFGETGALMELPRDHTARACAPLSVQSF
                     RRSEFPELVQRVPSFFFFLAEKLTNRLYQAKELGRSPNSFLELTGSLSNFDVVTVYQT
                     IIQSMQTGLLSIADEAGETISTFYFEKGTPRWGRFEHLSGEEAFWQLFLHEHRSGNFF
                     FSNQTQVRADWGEGSEMSRHADEILINAIQMRDQFEDLRKRMTDSSVQLKRQKLNLAW
                     DNPKIEELRPVAEEIWQLAYNTPVTLADLHGQSRFCDWKIYRTVDELVRNGHFTLGAN
                     CEEPQLVEAGS"
     gene            3329..>3712
                     /locus_tag="VNW28_02090"
     CDS             3329..>3712
                     /locus_tag="VNW28_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08740.1"
                     /translation="MNGLAYLFERFPSFTQTFCYREIDELQRQGVSPAIFSVRRPADE
                     PAQDWDASIVRQVQYLPGDEQVVREVDRALCKGALPKPVQREITTWGRQTDFLRLYQA
                     AHLGPRLVTLGVRHVHAHFAGLAART"
BASE COUNT          683 a         1088 c         1207 g          734 t
ORIGIN      
        1 gaacggttcg ccctggcgat cttcatccgc gccgggcgct tcgccgatga acatcagttc
       61 cgcgttggga ttgcccaccc cgaaaacggt ctgcgtgcgc gcggaggcga ggtgcgggca
      121 ccgaaggcaa actgagacgc gctggcggat gggctccagc cgttgggcct tgtcggtcgc
      181 gagcggcggg aggtggcaaa gccgatcgag cgcgttgcga ctggcgcgga gcggtcgatc
      241 cgtcgcgcgc tccatctcgt cgagcgctgc gatcgtggcg ttgagggcct gggtaaactc
      301 gtccatgcgg cggattcatt cttcaaggtt ttgcgcgtgg cggcaattcc tttgatggtg
      361 cggcgatgag cggccgagtg gacgagtacg atttcgcgtt accggaggaa ttgatcgcgc
      421 agcgaccgct gcctcggcgg gaggatgcgc gcatgatggt gctcgatcgc gcccgcggaa
      481 cgatcgcgca ccggcggttt caggagctgg gggagttcgt taggccgggc gatctgctgg
      541 tcctcaacga tacccgtgtc ctgccggcca ggcgtttttc cgacgacggc gcgatcgagt
      601 ttctcttgct cgagcaggtc gatccgcaaa tttggaaatg cctggtaaag ccggggcgca
      661 agatgcgtct cggcgcgacg gcggtgctcg acggggtcac cggccgcgtg gagaaaatcc
      721 tggacgaagg cgagcggctc gtgcgcttcg agcaggcgat cgatccttat cacgggggag
      781 atgttccctt gcccccgtat ctccagcgcg cggcggacgc ggaagatgcg gcgcgctacc
      841 agacggtttt cgcgaagacg cccggggcgg tcgcggcgcc caccgccggt ttgcatttca
      901 cccaggaaat tctggcgaag ctgccgcata ccttcgtcac tttgcacatc ggtacgggaa
      961 cttttcggcc ggtgcagagc gcgacgatcg gcgggcatcg gatgcatgcg gagcggttca
     1021 cgatcacgga ggaagcggcc gccgcgatca atgcggcgga gcgagtgctc gcggtgggga
     1081 caacgtccgt gcgagtgctc gaggcggccg ttaggcggaa gacgcgattg attgcccagg
     1141 aaggtgcgac cgacattttc attcatccgc cgcacacctt ccgggccgtc gaccggttat
     1201 tgaccaattt tcatcttccg cggtccacgc ttttgatgtt ggtggcagcc tttgccggtc
     1261 gggaatttat cctccgggcg tatgccgaag cagtcggcga gcgctaccgc ttttatagct
     1321 acggcgattg catgttgatc ctgtagacgt ttcgcggtgc gaagcgcgga aggaatcgag
     1381 gcgcggagat tgttccccca aatcacccgc aggaccgcgc tgcgcagtct tcggctcgga
     1441 gagagcgaac cggctacaac tcgccgcgct gcatccttat gttactctcg cggatggagt
     1501 catccgaaac ttccgacaaa ccgaagggcg aactcgtgac gcgcaccgtc gccatgccga
     1561 aggataccaa cccgaacggc gacatttttg gcggctggct gatgtcgcaa atggatctgg
     1621 gcagcggcat tcttgcttcc aagaccgcgc gcactcgcgt ggtgacggtc gcgctggacg
     1681 ggatgtcgtt cctcgagccg gtaagggtcg gggacacggt cgcctgttat gcctgggtcg
     1741 aatcgatcgg ccgcacgtcg atgaagatcc cggtagaagt atgggtgcag cgctacatgc
     1801 gcaccgaaca gacccgggtc acgcacgcgg tcttcaccta cgtggcggtc gatcaggccg
     1861 ggaaaccgat cccggtgccg cgcgactagc ccggaaaatc ttcgagcgag gtcaggcgcc
     1921 gttcagtgac cactgccgtc gctgactgac tgcgggccgg agccggaagc ctccgtcgca
     1981 atcaccggct cgctcggctg cgtctcgggg attttcactt tctgcggtcg caacttcttt
     2041 tgctgtttga cgcggcccag gctcgatttt tgaacgtagc gcgcgtaggc gtgctctcgc
     2101 cgcccgttcg ccgtcagcat cgaacacccg gaaagaaggg taattccaac caggagcagt
     2161 gcagccgcgc gaagtttttc tttcatgatc ctgcctcgac cagctgtggc tcctcgcagt
     2221 ttgcgccaag cgtgaaatga ccgttgcgca ccagttcgtc caccgtccga tagattttcc
     2281 aatcgcaaaa acggctttgg ccatgcaagt cggcaagtgt gaccggtgta ttataggcga
     2341 gttgccagat ttcctcggcc acggggcgca gctcctcgat cttgggattg tcccacgcga
     2401 gattcaattt ctggcgcttg agttgcaccg agctgtcggt catccgtttg cgcagatcct
     2461 caaactgatc gcgcatctgg atggcgttga tcaaaatctc gtccgcgtgg cgggacatct
     2521 cgctaccctc gccccagtcg gcgcgtacct gggtctgatt ggagaagaag aaattaccgg
     2581 aacgatgctc gtggagaaag agctgccaaa acgcttcctc accgctcagg tgctcgaagc
     2641 gaccccaacg cggtgtccct ttttcaaaat agaaggtgga gatcgtttcg ccggcttcgt
     2701 ccgcgatcga gagcagaccg gtttgcatcg actgaatgat cgtttggtaa acagtgacaa
     2761 cgtcgaagtt cgacaggcta ccagttaact ccaggaagct gttgggcgag cgccccagtt
     2821 ccttggcctg gtagagtcgg ttcgtcagct tctccgcaag aaagaagaaa aacgagggca
     2881 cccgctggac gagctccgga aattcgctcc ggcggaatga ttgcacgctg agcggggcgc
     2941 aggcgcgggc ggtgtggtcg cgcgggagtt ccatcagggc gcccgtctcg ccaaagatgt
     3001 cgccgcggga aaggacggtc gtcggcgacc cggggcgggc attgtgcggc aggatctcga
     3061 cggctccgcg attgatgatg aaaagctcat cgccgacttc gcccgaggaa taaacgaggt
     3121 cgttttccgc gaaatgcctt actttcgaaa ggatgccgat ggccttcagc tcgatcaggt
     3181 ggagcgaagt gcaaaaggcg aagaaatcgc gctggagcat tcctaaataa tagcaagagt
     3241 cgtgcggggg ccgattgaac cgctgagaaa aatccatggg ccggaactgc ccctttccaa
     3301 gaccgggatt cgccggtaat cctttgccat gaacgggctc gcctatcttt tcgagcggtt
     3361 tccctcgttc acccagacct tttgctaccg cgaaatcgac gagttacagc ggcagggcgt
     3421 ctcgccggcg atcttttccg ttcggcgccc ggcggatgag cccgcgcagg actgggacgc
     3481 gtcgatcgtc cgccaggtgc aatacctgcc gggcgacgag caggtggtac gggaagtgga
     3541 ccgcgcgttg tgcaaaggcg cgctccccaa gccggtgcag cgagaaatca cgacctgggg
     3601 ccggcaaacg gacttcctcc ggctttacca ggcggctcat cttgggccgc gcctggtcac
     3661 gctcggcgtg cgacacgtgc atgcgcactt tgccggattg gcggcccgca cc
//
LOCUS       DATMNS010000054        21436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4331051, whole genome shotgun sequence.
ACCESSION   DATMNS010000054 DATMNS010000000
VERSION     DATMNS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 21436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21436
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4331051"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1282
                     /gene="odhB"
                     /locus_tag="VNW28_02095"
     CDS             59..1282
                     /gene="odhB"
                     /locus_tag="VNW28_02095"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HXA08741.1"
                     /translation="MSIEVKIPPVGESISSGVVSVWHKKSGDYVAAGEALLTLETDKV
                     STEITAEKGGVLETKVDEGQEVKIGEVVALIDDQATSPAKTKATAPNEAVPAAKAEKH
                     SANADVLSPAVKRMTTEEKIDPGQITGTGKGGRLTKGDVISHLQAAPQTLTDDQEKPR
                     GRKQPASEAPAPSGNERFTRKKMSPLRRKIAAQLVMAQQTAAILTTFNECDMSEVIAL
                     RKKAQEKFTADHGVKLGFMSFFIKATVEALKAVPSINSRIEGDDFILNHYYDIGVAVG
                     TERGLVVPVVRDADKKSFADLERDIADYASRARDGKIKIEDLQGGTFTISNGGVYGSL
                     MGTPILNPPQSGILGMHAIQQRPVAREGKVVVRPMMYLALSYDHRAVDGKEAVTFLVK
                     VKEAIENPTVLLLDL"
     gene            1404..2810
                     /gene="lpdA"
                     /locus_tag="VNW28_02100"
     CDS             1404..2810
                     /gene="lpdA"
                     /locus_tag="VNW28_02100"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXA08742.1"
                     /translation="MDKFDVIIIGSGPGGYVAAIRAAQLGLKTAIIEKDHELGGTCLN
                     IGCIPSKALLASSDHFVFAQKEAAKHGIVLGKVGLDLPTMLARKDKVVQTLTGGVRAL
                     MKANKVITFTGLGTITSPGQVRVQPNDGAATEIAADHIVIATGSVPIDLPSMPFDGKT
                     IVCSTEALSFDKPPEKFLVVGAGAIGLELGSVWNRLGSEVTVLEFLPRIALGFDLEVS
                     NLLQRALTAQGIKFHLETKVGSVNVANGGAIATATKGGEELNFEADKVLVSVGRRAFA
                     EGVGADTIGVALDERGRIKVNEKFQTNVAGIYAIGDVIPGPMLAHKAEEEGIACVELI
                     AGKAGHVNYDVIPGIIYTNPELAGVGITEDSAKEQGLKVRIGKFPFRANGRAVANADV
                     EGVVKFVADAETDRILGASILSHAASELIAEIVAVMEFGGSSEDLGRTVHAHPTLSEA
                     VKEAALAVEKHAIHIGNR"
     gene            2824..3099
                     /locus_tag="VNW28_02105"
     CDS             2824..3099
                     /locus_tag="VNW28_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08743.1"
                     /translation="MADDSEKLSGAEEYIAVEIELIEALDPDQEKNLRAALEKLEPHA
                     FDSCEIDPRKISLCYDPTRTSQKDLLQLIKQAGGKLKHIESESSPLL"
     gene            complement(3190..4341)
                     /locus_tag="VNW28_02110"
     CDS             complement(3190..4341)
                     /locus_tag="VNW28_02110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXA08744.1"
                     /translation="MDNGNLELIQIIQDGLRARGPAPFAWFMEQALYHPEHGYYGSGR
                     CAIGRRGDYFTSVSVGPLFGKLVAGQFAEIWEALDRPNDFVIVEQGAHHGEFAADVLD
                     ALRAGYPECFAELRYRIVEPFPILRRRQEETLGSFSEKTEWVASLEEMERFCGVHFSN
                     ELLDAMPVHLLRAAGEDATRRWYERFVDQTAGGFTLVDRPVADARLRERLAKIPPPPA
                     ESYETEINLVALDWIAALAVKLRRGAVLVADYGLVRDEFYAPHRRRGTLQCYAGHRVL
                     PSLLEKVGTSDLSAHVEWTSLAERAEECGLRLAGFTDQHHFFTGLLASHPELASAGAG
                     KSRALQTLIHPEFLGTRFQFLALAKEFPPATSLGGFKFARDARAALGLV"
     gene            4494..7034
                     /locus_tag="VNW28_02115"
     CDS             4494..7034
                     /locus_tag="VNW28_02115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXA08745.1"
                     /translation="MPVDYFTPTEFSRVHRPWYTRPWLFLPLIIGVVVMVAALLAFLP
                     VAASLEQQAAGFDLSHLEQMESASVIVDRNNKIFGQIYVENRETVPYDQLPPNLVNAV
                     IAIEDNKFYQHHGYDLLGIARAALKNAAAGHVRQGASTITQQLARNSFDLKGKTFRRK
                     LLEMFVARRIEDHFSKRKIMELYLNRIYFGGGLYGAEAAARGYFDKPAREMSLAECAT
                     LAGLVKSPNKLSPWADRAASREARNFVLSRMRELGFITAEQHEGAQAEDLVIGNRQNA
                     RGQNYAIDYIRQQVIAAVGWDRAMSDGFRIHTTIDAHLQDVAEKSLRAQLDAAEETPG
                     YHHQTYTQYAALTQEARKNNSPKGKPGAPPEYLQGAVIALDNKTGGILALVGGRDFEH
                     NEYNRALQARRPPGTAMLPFVYATAFENGLFPGSLVEDSALDNRTVMIGGTTGILGEW
                     GPEDANNRYEGPITAREALAKSKNGAAVRVGMTAGLEAVTQLVHAAGVETKLRSYPST
                     FLGSSEMTLAELALAYTIFPNEGWRPAPPHILDRIEDKEGTIVWRAEHSRAEKPVLKP
                     EIAAEVTSCLTDALAEGTGQVAYQKYGLKKFPAAGKTGTAYDFTDALFAGYDSALTCV
                     VWAGFDKPQKIYRGAFGSVLALPVWVDVMNASIAKYPPQEFPMPSGLRKIEICSRSGL
                     LATDRCYEDVRSASGEVVKRRTSYFELATAGQMPTDPCDVHTDSVRTQLAKKFEDSQW
                     PRAALAIDTKEVAPVIMRAPTLVAAEDPYNAVGSTSQPKALAKAGDKSVATFDPDKPI
                     KRALPVTPEPAAPVEVRRAQPVRPIDEAGTEQLLQNEPPPPLDFGDDH"
     gene            7021..8055
                     /locus_tag="VNW28_02120"
     CDS             7021..8055
                     /locus_tag="VNW28_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08746.1"
                     /translation="MTTTERGRVDGFRLTVLNPGGRDPAQDFSRGVPGPNDSGHAPVN
                     FHGYTACTGGAFHCDARRAIAEDQPVLLLLRGDFKATERALGALKKHGLTVAVSLKET
                     GLHQIAQQLAAPKRAALFREIVRTADGCLAATSEALPFYGTGAFIPTPYPIDDPRWDF
                     SRPFGERRGIFVGTREWDVPSRHHLAALQIALELDEPVTLFDENPRRCRKILATLGVP
                     EGKVTIHARRLPYRDYLAEMARHKIVLQADKSAVPGQVAGDALLCRLPCVGGDGAVDR
                     LGFPRTSGHGRSLGELKELATRLLADPEFHAEITAESQERARELLGFKTVARQLAQFF
                     GSLAQRPSAS"
     gene            complement(8034..9191)
                     /locus_tag="VNW28_02125"
     CDS             complement(8034..9191)
                     /locus_tag="VNW28_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA08747.1"
                     /translation="MPERSPPVVACYCATFLKPEMLHIYRQITALERFRPVVIAQKRE
                     ETERFPFAPVFVVGKPATHFLRRFWFRTLQDAPWQMSRGEVTQIERLLATNDAQLLHI
                     YFGHIAVHLLPLIRHWPKPTVVSFHGADVLVDLDKPRYRSATREMLTAARLVLVRSQS
                     LARAVQKLGCDEGKIRLHRAGIPLTEIPFRPRVWPKDGVWKFLQAGRLIEKKGFQTSL
                     RAFAVFAAKNSGAILTIAGEGPLLLELRNLARELGLKERVRFVGFLSQAQLREQFYES
                     HIFLHPSELGGDGNQEGVPNSMLEAMASGLPVFATNHGGIPEAITPEVSGVLVAERAH
                     AALAAALLAWTDRPEALAQLARRGAAAVAEKFEQRAQARALEDCYQEALGR"
     gene            complement(9248..9580)
                     /locus_tag="VNW28_02130"
     CDS             complement(9248..9580)
                     /locus_tag="VNW28_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08748.1"
                     /translation="MKKKLLISMLLALTTGMAFAQAQVQGKLSTTAKARSNGELSTTL
                     PKPRPPAAAPFVPTEGAVQVAIRTGRPIQLINPAAPARYGNAQEHVMHDPNDPGKPKG
                     ISLFAWSF"
     gene            9656..10627
                     /locus_tag="VNW28_02135"
     CDS             9656..10627
                     /locus_tag="VNW28_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HXA08749.1"
                     /translation="MDYIEKAARVLDLEISELQRLRQRLGESFARAVEVIKKTVEARG
                     KIIVLGVGKSGHIGGKIAATLTSTGSPAVVLDSLNALHGDLGVIADGDVVLALSYSGE
                     TEELLRILPAIARFQVGVIAICGAADSTLAQNCDVFLDVNVEQEACPLNLAPTSSTTV
                     MLALGDALAMVLLEARGFQKEDFAKFHPAGMVGRSLLLRVHQIMRPRSALALVRPETT
                     VRTALELMTSLRAGAAVVAGEDEQLLGIFTHGDFVRHYQNDSHVGERFVADLMTLNPV
                     VVRQDKLAVEVLNLLERHRIDDLVVVDERGVPVGIVDSQDLTKLKLL"
     gene            10775..11332
                     /gene="efp"
                     /locus_tag="VNW28_02140"
     CDS             10775..11332
                     /gene="efp"
                     /locus_tag="VNW28_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXA08750.1"
                     /translation="MAAANDLRKGMAIKYNGNTAIVLDVHHRTPGNLRAFVQAIIRYI
                     NTGKSADVRFGSTDKVELVDISRSTLEFSYKDNTGFHFMDPSTYETITLQESLLAEAK
                     DYLVENLPVEVLFTEGRPVQVELPSSVQLKVVEAPEGLRGDTASNVTKPALLETGKTV
                     NVPLFINEGETVKIDTRTGAYMGRA"
     gene            11336..12133
                     /locus_tag="VNW28_02145"
     CDS             11336..12133
                     /locus_tag="VNW28_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08751.1"
                     /translation="MAARRVTRHPARGRVKVAPYVVPTRRGTPPAGSLMVPTKWVKFV
                     VALFLLPLCAVLSQTFFTSFARATVAERLWAGEEFWFFSLGAVLWLIAFFGLPRPIVL
                     YVFGHELTHALWVWLMGGRVSRFRVGREGGHILTDKNNFLIALAPYFFPLYSILVLAI
                     YGALSLCVNLQPYGRLLYALVGVTWAFHFTFTCWMIPKNQSDLRDNGTIFSLVVIYLM
                     NLLLLSAMLIIASPNITFAGFGADMVRNIANFSHAVVNLAERFSRGA"
     gene            complement(12158..15031)
                     /gene="treY"
                     /locus_tag="VNW28_02150"
     CDS             complement(12158..15031)
                     /gene="treY"
                     /locus_tag="VNW28_02150"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HXA08752.1"
                     /translation="MNPDHPRIPSSTYRLQFNREFTFAQAREIVPYLHALGISDCYAS
                     PYFQARAESLHGYDITDHNQLNDAIGSRAEYDAWVAELHARAMGQMLDFVPNHMGIVE
                     PGNLWWADVLENGPSSFYAPYFDIDWHPLKSGLRGKVLLPILGDQYGRVLERGELVVH
                     FESGRFFLRYFEHEFPIAPGTYRHVLQIALEQLSAHQAEEFYAELQSILTALEYLPRR
                     TETDPERIAERTREKEIIKRRLERRCQEAPEVQAAIEKALTIINGQPGDARSFDGLDE
                     MLNEQSYRLAFWRVAAEEINYRRFFDVNDLAAIRMEVPEVFEATHQLLLELVGTGAVT
                     GLRIDHPDGLYLPQEYISKLQGRCAEGLQAEGRASASPGLAVASPSRRAIYLVVEKIL
                     SGTEQLRSDWLVHGTTGYDFMNDAIGVLVDGSAERAITSTFHKFIGHTLQFGHLVYAK
                     KRLVMRLSLANDVNVLGAMFDRLSEKNRWYRDYTLDTLTLAVRETIACFPVYRTYLAP
                     DRPVNDADRAVIERAVAAAKRRNPALEESVFNFLRDILLFRFPENLSEEERADHVHFV
                     LKFQQSTGPIMAKGLEDTVFYIFNRLAALNEVGGEPQRFGLDVEAFHRRNAERQAHWP
                     AALLATSTHDTKRSEDVRARMVAIAERPEEWRRSLGRWRTLNKRWKKSVDENEAPDAN
                     EEYLLYQILLGTWPLEPFRELSAAARGAYLARIQQYMAKALKEAKLKTSWVQPNELWD
                     SAVMDFVAQILDPAPRNRFLESFIPLAEEVARTGAINALAQTLLKLTSPGVPDIYQGN
                     EIWDFSLVDPDNRRPVDYARRQAMLDSLAGATPEDLLGNWTDGRIKLFLTQRLLTFRR
                     DHAALFRDGRYTPLSLTGSRADCCIAFTREHEGHSIIVLAPRLSSRVGFPAIGPAWGD
                     TAVNLPAGFTAGRDLFTGETKEEKNSALALAEAFARLPFAVLI"
     gene            complement(15140..16846)
                     /gene="argS"
                     /locus_tag="VNW28_02155"
     CDS             complement(15140..16846)
                     /gene="argS"
                     /locus_tag="VNW28_02155"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXA08753.1"
                     /translation="METFQTLLTRELLRALAASGLPAKGEVVPATDARFGDYQTSVAL
                     VLAKQRGENPRALAQKIIDRLEVAEWCEPPTVAGAGFINFALQPAAVAAKAAQLLQDE
                     RLGVPLAEKPHRIVIDFGSPNVAKPMHIGHIRSTVLGDALARIAQFLGHDVIRDNHIG
                     DWGTQFGMVIYGWKNLLDRAALARDPIGELVRIYKTAHELTKSDPAVRDACRNELVRL
                     QAGDEENLRIWNECVALSRQEFEATYRILDIHYDLQRGESFYNDRLSAVVDRLLKSGL
                     AEISEGAVCVFFRDIPELAERPCIIRKSDGGFNYATTDIATVDYRINDLKADAVWYVV
                     GAPQTLHFKQIFEIARREGYTADFRHITFGSILGADRKLMKTRSGDNVPLCEVLDEAV
                     ARARKIVEEKNPDLAEAEKNAIAQTIGLGAVKYADLSQYRMTDYIFSWDRMLSLQGNT
                     APYLQNAYVRIRSIFRKAGEAFSAPNELTLVEPAELNLAKRLTQFAESVPQVLHDFRP
                     NILANYLFELANAFHAFYEACPVLKADEPARATRLALSELTARTLQRGLDLLGIRVPE
                     KM"
     gene            complement(17027..17491)
                     /locus_tag="VNW28_02160"
     CDS             complement(17027..17491)
                     /locus_tag="VNW28_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXA08754.1"
                     /translation="MSLASLLTSEQIIPEMKATERWPAIVELIDLLVGQGKIDPANRE
                     SILASLKQREETMSTGIGFGIAIPHASSEAVAEVVAGFGRSSKGIEFDALDNAPVKFV
                     VLFIVPKNQFQTHLRTLASIAKFLNDRSVRESLASAGTKEEILAIFQERSQK"
     gene            complement(17594..18301)
                     /locus_tag="VNW28_02165"
     CDS             complement(17594..18301)
                     /locus_tag="VNW28_02165"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXA08755.1"
                     /translation="MSSGPSHPEVSEVVPLFNEEENVPILQAELAAALAGLEHEIIFV
                     DDGSTDGTLARLTSGSAVRVLRFAQNAGQSAALYAGANAARGATVVLIDGDLQNDPAD
                     IPRLLAEIERGADLVCGYRAQRKDTLVKRLTSWIANFVRSRFTKDGVRDTGCTLKAMR
                     RECVRTLVPFKGMHRFIPALVKGAGYRLVEVPVNHRARKFGQSKYGLGNRAVRATIDM
                     FGVRWLLSRQLTYRLKD"
     gene            complement(18298..19872)
                     /locus_tag="VNW28_02170"
     CDS             complement(18298..19872)
                     /locus_tag="VNW28_02170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXA08756.1"
                     /translation="MSTTRAVWIFLFVLTVVRLALLNATQLSPDEAYYWMWGQRPALS
                     YFSKGPGVALVIRSSTALFGDNEFGVRFWSPLFAAGTSLLLYYFASRLFSALAGFWTV
                     VALSITPIFNLGSLVLTIDPLSIFFWVAAFYTFWLALERSPRFSWWWPFTGLLIGLGF
                     LCKFTNALELISILLVLLLTRDLRREFVRPSLYLLLFVCALCTLPPLVWNSQHAWITL
                     AHLKARGSLNHAPGFHPLELLSFLGEHFVTYSPLLFLGLVWAVVASWRRARQNFKVLY
                     LLWFGLPVFLFYTILSLNKAAAPNWDGLAFLSLGILAISYWRERSAARVAAQNWTRVA
                     VVLGLVMSALLVALLVNNRGLDFRGRDPSDRLRGWRSFAEAVNQVRSDFEKQTGKPVF
                     LIADARDRASELSFYLPDKRVEGPGHPPVYLPESQDMVNQFSFWPRYDEFVPLPPNAA
                     RDANEVYTEEQGENVFVGRTALYLEQGKPERAPRNIRGAFERMERVATIEVRTGGRLL
                     RTIQVYACYDYRTMPL"
     gene            complement(19869..20519)
                     /locus_tag="VNW28_02175"
     CDS             complement(19869..20519)
                     /locus_tag="VNW28_02175"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXA08757.1"
                     /translation="MDQILFHLINEQWTHPALDLFMAAISNVEIWKPLFVLLALSALI
                     FGGFKGRALVFCIGFVLLVNGSLTSGLKKLVDRRRPKQVERVRMVELAKARPEFLTLF
                     KKVEIRYSDARDRDRSGPSFPSGHVVNNVSVALILTFFFRRWGWLYFLLAAAIAWSRI
                     YLGAHWPSDVLATFFLAAGETALLLALLETVYRRAAARWAPQFFVRHPRLVGDSIS"
     gene            20601..21284
                     /locus_tag="VNW28_02180"
     CDS             20601..21284
                     /locus_tag="VNW28_02180"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HXA08758.1"
                     /translation="MRKLIILVAGAVLAGMAAHVPAEPPSAPEILDQVRLQQSQQQLD
                     LQGQLRTGATVIPFRMTQTGPVIRYTFANPPEVLQLRLGEKNSRLDLVTESSTRKFSA
                     SRLDEKIGGTGVTYGDLALKFLYWPNAQVIGADNIRTRDCWKLRLPGPGKDAQYSSVL
                     LWVDKESGAIMQLEGYDQTGRLAKRFEVVSAQKIEGRWFLKQMRIEELQPGTTKVLVR
                     TYLEIKKPG"
BASE COUNT         4378 a         6713 c         6444 g         3901 t
ORIGIN      
        1 acggcgacag gaattttttc gaccgcactg cgctcttcgg cttaaggttg cggcagccat
       61 gagcatcgaa gtcaaaatcc cgccggtcgg cgagtccatt tccagcgggg tcgtctccgt
      121 ctggcacaaa aaatccggcg attatgtcgc agcgggcgaa gcgctcctga ccctcgagac
      181 cgacaaggtc tcgacggaaa tcaccgccga aaaaggcggc gttcttgaaa ccaaagtaga
      241 tgaaggccag gaagtgaaaa tcggcgaggt cgtcgcgctg atcgacgacc aggcgacctc
      301 cccggccaaa accaaggcga ccgcgcctaa cgaagcggtc ccggccgcga aggcagagaa
      361 gcattccgcg aatgcggatg tcctatcgcc cgcggtcaaa aggatgacga ccgaagagaa
      421 gatcgacccc gggcagatca cgggcaccgg caaaggcggt cgcctcacga aaggcgacgt
      481 gatctcgcat ctgcaggcgg cgccccagac tttaaccgac gatcaggaaa aaccgcgagg
      541 acgaaagcag ccggcgagcg aagcaccggc cccgagtggt aacgagcgtt tcacccgcaa
      601 aaagatgtcg cccctccggc gcaagatcgc cgcccagctc gtcatggcgc agcagaccgc
      661 ggccatcctt accaccttca atgagtgcga tatgagcgag gtgatagcgc tgcgcaagaa
      721 agcgcaggag aagttcacgg ccgatcacgg ggtcaaactc ggtttcatgt cgttcttcat
      781 caaagcgacg gtcgaagcgc tcaaggcagt gccgtcgatt aacagccgga tcgaaggcga
      841 cgatttcatt ttgaatcatt actacgacat cggcgtcgca gttggaacag agcgcggcct
      901 ggtcgtgccg gtggtgcgcg atgcggacaa gaagagtttc gccgatcttg agcgggacat
      961 cgccgattac gcgagccggg cgcgcgacgg caagatcaag atcgaggacc tgcagggcgg
     1021 caccttcacc atttccaatg gcggcgttta cggatccctc atgggcacgc cgatactcaa
     1081 tccgccccag agcggcatcc tcgggatgca tgcgattcag cagcggccgg tcgcgcgcga
     1141 aggcaaagtc gtcgttaggc cgatgatgta tctcgccctg agctacgatc accgcgcggt
     1201 cgacggcaag gaagcggtca cctttctcgt caaagtgaag gaagcgatcg aaaacccgac
     1261 cgtgctgttg cttgatttgt agttactgcg cttgtcgggc gttacaggaa cgagttcgcc
     1321 ggggtccgcc cccggctaca actactgcgt cgggatgaca agccagagcg acacggctaa
     1381 ataagagctg acctcattat tccatggata aattcgatgt cattatcatc ggctccggcc
     1441 cgggcggcta cgtcgcggcg atccgggccg cgcagctagg actgaaaacc gccatcatcg
     1501 aaaaggacca cgaactcggc ggcacctgcc tcaacatcgg ctgcattccg agcaaagcgc
     1561 ttctcgcctc gagcgaccat tttgttttcg cgcaaaagga agcggccaaa cacggcatcg
     1621 ttctcggcaa agtcggcctc gatctcccca ccatgctcgc tcgcaaagac aaggtcgtgc
     1681 aaactctgac cggcggcgtg cgggccctga tgaaggccaa caaagtcatc accttcaccg
     1741 ggctcggcac gatcacctcg ccgggccagg tgcgcgttca gcctaacgac ggcgcggcga
     1801 ccgagatcgc cgccgatcac atcgtgatcg cgaccggcag tgtgccgatc gacctgccca
     1861 gcatgccatt cgacggaaag accatcgtct gcagcactga ggcgctctct ttcgataaac
     1921 cgccggaaaa attcctggtc gtcggtgcag gcgcgatcgg actcgagctc ggctcggtct
     1981 ggaatcgtct cggctccgaa gtcacggtcc tcgaatttct cccacgcatc gcccttggtt
     2041 tcgatctcga agtctccaac cttctccagc gcgcgctcac ggcccaggga atcaaattcc
     2101 atctcgaaac caaagtcggc tcggtcaacg tcgcgaacgg cggggccatc gccaccgcca
     2161 ccaaaggcgg cgaagaactc aacttcgagg ccgacaaagt cctcgtctca gtcggtcgcc
     2221 gcgcctttgc cgaaggcgtc ggcgccgaca cgatcggcgt cgcgctcgac gagcgtggcc
     2281 ggatcaaagt gaatgagaag ttccagacca atgtcgctgg catctacgcg atcggcgacg
     2341 tcattcccgg tccgatgctc gcgcacaagg cggaggagga aggcatcgcc tgcgtcgaat
     2401 tgatcgcggg caaagcaggg cacgtgaact acgacgtcat ccccggcatc atttacacca
     2461 acccggaact ggccggcgtc gggatcacgg aagattccgc gaaagagcaa gggctcaagg
     2521 tacggatcgg caaattcccc ttccgcgcca acggccgcgc tgtagcgaac gctgatgtcg
     2581 aaggcgtggt caaatttgtc gccgacgccg aaaccgatcg catcctcggt gcgtcgatcc
     2641 tctcgcacgc cgcctccgaa ttgattgccg aaatcgtcgc ggtgatggag ttcggcggga
     2701 gcagtgaaga cctcggccgc accgtccacg cgcatccgac cttgagcgaa gcggtcaagg
     2761 aagcggcgct cgcggtcgag aaacacgcca tccacattgg caaccgctaa cgatttacca
     2821 ggtgtggcgg acgattctga aaagctttcc ggcgcggaag aatatatcgc cgtggagatc
     2881 gagttgattg aggcgctcga tccggatcag gaaaagaatc tgcgcgcagc tttggaaaaa
     2941 ctcgagccgc acgctttcga ttcctgtgag atcgacccga ggaagatctc gctttgttac
     3001 gacccgaccc ggacctcgca gaaagacttg ctccaactga tcaaacaagc cggcggcaaa
     3061 ttgaagcata tcgagagcga aagttcgccg ctgttatagt cgaggcaaat ttgcaaagtg
     3121 ggcggggcct ttgtgcctga tcagagggcg cagcgcaacc ccatccgggg cacaaaggcc
     3181 cctcccacat taaaccagcc ctagcgccgc gcgcgcgtcc cgcgcgaact tgaagccgcc
     3241 taacgaagtg gccggcggga attccttcgc gagcgcgagg aattgaaacc tcgtgcccag
     3301 aaattccgga tgaatcagcg tttgcagggc gcggcttttt cccgccccgg cggaggcgag
     3361 ctcgggatga ctggcgagca gacccgtgaa aaaatgatgc tgatcggtga aaccggcaag
     3421 gcgcagaccg cattcctcgg cgcgctcggc aaggctcgtc cactcgacat gggcgctcag
     3481 gtcgctcgtc ccaactttct ccaaaagcga gggcagcacc cgatgcccgg cgtagcattg
     3541 cagtgtcccg cggcggcgat gcggcgcata aaattcgtcc cgcaccaacc cgtaatcggc
     3601 tacgagcacc gctccgcggc gcaacttgac cgccagcgca gcaatccaat ccagtgccac
     3661 caggttgatt tccgtttcgt agctttcggc cggcggcggc ggaattttcg caagccgctc
     3721 gcgcaaccgc gcgtccgcca ccgggcgatc gaccaacgtg aagccgccgg cagtttggtc
     3781 gacaaaacgc tcgtaccagc gacgcgtcgc gtcctccccg gccgcccgaa gcaggtggac
     3841 cggcatggcg tccaacagct cgttggaaaa gtggacgcca caaaatcgct ccatctcctc
     3901 gagcgacgcc acccattccg tcttttccga aaatgagcca agcgtttctt cctgccgccg
     3961 ccgcaagatg ggaaacggct ccacaatccg gtagcgcagc tccgcaaaac actctggata
     4021 gcccgcccgc aaagcgtcca gcacatcggc cgcgaattcc ccgtgatgcg cgccctgctc
     4081 gacgatcaca aaatcgttcg gccgatcaag cgcttcccaa atctccgcaa attgcccggc
     4141 caccaatttg ccaaacagcg gccccacact cacgctcgtg aaataatctc cgcgccgccc
     4201 gatcgcgcac cggccggagc cgtaatagcc atgctccgga tgatagagcg cctgctccat
     4261 aaaccacgcg aacggcgccg gccctcgcgc ccgcaggccg tcctggatga tttgaattag
     4321 ttccaaattg ccgttgtcca tggggcaccc ttcggcccgt catgcgcgga ttccagccca
     4381 gcgcgggcgc gcatcggttc ctaccattac cactccaatt tggattttgc agaagtttgc
     4441 cgctaagttg cgcgcgattg ccaaatcctt tgggctcccg cccacctcgc cgcatgcctg
     4501 ttgattattt cacgccaacg gagttttccc gcgtccaccg tccctggtac acgcgtccgt
     4561 ggcttttcct cccactgatc atcggggtcg tcgtcatggt cgccgctctg ctcgccttcc
     4621 tcccggtcgc ggcctcgctc gaacaacagg ccgccggctt cgatctctcg catctcgagc
     4681 agatggaatc ggccagcgtt attgtcgatc ggaacaacaa aatcttcggc cagatctacg
     4741 tcgagaatcg ggagaccgtc ccctacgatc agttgccccc caacctcgtg aacgcggtca
     4801 tcgcgataga ggacaacaag ttttaccagc atcatggcta cgacttgctc ggcattgcgc
     4861 gcgccgcgct caagaacgcc gcggccggtc acgtgcggca gggtgcgagc acgatcaccc
     4921 agcaattggc gcgcaacagt ttcgatctca agggcaagac gtttcgccgc aagctgctcg
     4981 agatgtttgt cgcccgccgg atcgaggacc atttctcgaa acgcaaaatc atggagcttt
     5041 acctcaaccg gatttatttc ggcggcggac tctatggggc ggaggcggcc gcgcgcggct
     5101 atttcgacaa gccggcccgc gaaatgtcac ttgccgaatg cgccaccctc gccgggctgg
     5161 tcaagagccc gaacaagctc tccccgtggg ccgatcgggc ggcctcgcgc gaggcgcgca
     5221 acttcgttct ctcccggatg cgcgaactcg gcttcatcac cgcggagcaa cacgagggcg
     5281 cgcaggcgga ggatttggtc atcggcaacc gccagaacgc gcgcggacaa aattacgcga
     5341 tcgattacat ccgccagcag gtgatcgcgg ccgtcggctg ggatcgcgcg atgagcgacg
     5401 gcttccggat tcacaccacg attgatgctc acctgcagga cgtcgcggaa aaatcgctcc
     5461 gcgcccagct cgatgcggcc gaggagacgc ccggctacca tcatcagacc tacacgcaat
     5521 acgccgcgct cacgcaggag gcgcgcaaga acaattcgcc taaaggaaag ccgggcgcgc
     5581 cgccggaata cctgcagggc gccgtcatcg cgctcgacaa caagaccggc ggcattctcg
     5641 ccctcgtcgg tggccgcgat ttcgagcata acgaatacaa ccgcgcgctc caggcgagac
     5701 gcccgccggg cacggcgatg ctgcccttcg tttatgccac cgccttcgaa aacgggcttt
     5761 tccccggttc gctggtcgag gattccgcgc tcgataaccg cacggtgatg atcggcggca
     5821 ccaccggtat cctcggggag tggggaccgg aggacgctaa taatcgttac gaaggaccga
     5881 tcacggcgcg ggaggcgctc gccaaatcga agaacggcgc ggcggtgcgc gtgggcatga
     5941 ccgccgggct cgaggccgtg acacagctgg tgcacgccgc cggcgtggaa actaaattac
     6001 ggtcctatcc ctcgaccttc ctcggcagca gcgagatgac cctcgccgag ctggcgctcg
     6061 cctacaccat tttccctaac gagggctggc gcccggcgcc gccccatatc ctcgatcgaa
     6121 tcgaggacaa ggaagggact atcgtctggc gggcggagca cagccgcgcc gaaaagccgg
     6181 ttctcaaacc ggagattgcg gccgaggtca cttcctgcct gacggatgcc ctcgcggaag
     6241 gaaccggaca ggtcgcctac cagaaatatg gtctgaaaaa atttcccgcg gccggcaaga
     6301 cgggcacggc ctacgatttt accgacgcgc tctttgccgg ttacgattcg gcattaacct
     6361 gcgtcgtctg ggctggtttt gacaagccgc aaaaaattta tcgcggcgct ttcgggagcg
     6421 tgctggcgct gcccgtctgg gtggacgtga tgaacgcctc gatcgccaaa tatccgccgc
     6481 aggaattccc catgccgtcg ggtttgcgca agatcgagat ttgctcgaga tccggtctgc
     6541 tcgcgaccga tcgatgctac gaggacgtgc ggagtgcgag tggtgaggtc gtgaaacgcc
     6601 gcaccagcta tttcgagctg gccacggctg ggcaaatgcc gaccgatccc tgcgacgtgc
     6661 ataccgattc cgtccggacc cagttggcca agaagtttga agacagccag tggccgcggg
     6721 cggccctcgc gatcgacacc aaagaggtcg cgccggtgat catgcgcgcg ccgaccctgg
     6781 tcgctgcgga agacccctac aatgcggtcg gctcgacctc ccaaccgaag gcgctggcca
     6841 aggcaggtga caaatccgtg gccactttcg atcctgataa accgatcaaa cgcgctcttc
     6901 cggtgacgcc cgagcccgct gcccccgtgg aagtgcgccg cgcgcagcca gtgcgaccaa
     6961 tcgacgaagc gggaaccgag cagttgctgc agaacgaacc gccaccgccg ctggattttg
     7021 gtgacgacca ctgaacgcgg ccgggtggac ggctttcgcc tcacggttct caatccgggc
     7081 ggtcgcgacc cggcgcagga tttctcgcgc ggcgtccctg ggcctaacga cagcggccat
     7141 gcgccggtca attttcacgg ctacaccgcc tgcaccggcg gcgcgtttca ctgcgacgcg
     7201 cggcgcgcga tcgccgaaga ccagcccgtg ctcctcctct tacgcggcga tttcaaggcc
     7261 accgagcgtg cgctcggtgc actgaagaag cacggtctca cggtcgcggt ctcgctcaag
     7321 gagaccggtc tgcaccaaat cgcgcagcaa ctcgccgcgc cgaaacgcgc cgcactcttc
     7381 cgcgagatcg tgcgcacggc ggatggttgt ctcgccgcca cttcggaggc gctgcccttc
     7441 tatggcactg gcgccttcat cccaacgccc tacccaatcg atgatccgcg ctgggatttt
     7501 tcccggccgt tcggcgaacg gcgcgggatt tttgtcggca cgcgcgagtg ggatgtgccc
     7561 tcgcgccatc atctcgcggc actccagatc gcgctcgaac tcgacgaacc cgtcacgctc
     7621 ttcgacgaaa acccgcggcg ctgccgcaaa atcctggcca cgctgggcgt gccggaaggc
     7681 aaggtaacta ttcacgcgcg acgtctcccc taccgggatt acctggcgga aatggcacgg
     7741 cacaaaatcg ttctccaggc cgacaagagc gcggtcccgg ggcaggtcgc cggtgacgcg
     7801 cttctttgtc gcctcccctg cgtcggagga gacggcgcgg tcgatcgcct cggttttccg
     7861 cgcacctccg gacatgggcg ttcgttaggc gaactcaagg aactggcgac gcgcttgctc
     7921 gctgatccgg aattccacgc cgaaatcacc gcggaatcac aggagcgagc gcgcgagctg
     7981 cttggcttca agacggttgc ccggcaattg gcgcaatttt tcggaagtct tgctcagcgg
     8041 ccgagcgctt cctgatagca atcttccagg gcccgggcct gggctcgttg ttcgaatttc
     8101 tccgccaccg cggcggcgcc gcggcgcgcc agttgcgcca gtgcctccgg ccggtcggtc
     8161 caggcgagca acgctgccgc cagcgctgcg tgggcgcgct ctgcaaccag aacgccgctc
     8221 acctcaggcg tgatcgcctc cggaattccg ccgtgattgg tcgcgaagac cggcagcccg
     8281 ctcgccatcg cttcgagcat ggaattcgga actccctcct ggttgccatc gccgccgagc
     8341 tcgctcggat gcaagaaaat atgcgactcg taaaactgct cgcgcaattg agcctgcgag
     8401 agaaaaccaa caaagcggac ccgctccttg agaccgagct cgcgcgcaag atttcgcagc
     8461 tcaagcaaaa gcggcccttc gccggcaatc gttaaaatcg cgccggaatt tttcgccgcg
     8521 aaaacggcaa aggcgcgcag gctggtctgg aatcccttct tctcgatcag gcgcccagcc
     8581 tgcaggaact tccagacgcc atctttaggc caaacccgcg ggcgaaacgg aatttccgtc
     8641 agcggaatgc ccgcgcggtg caggcggatc ttgccttcgt cgcacccgag tttctggacc
     8701 gcgcgggcga gcgattgcga gcgaacgagc acgaggcgcg cggccgtcag catttcgcgt
     8761 gtggcggagc gatagcgggg cttatcgagg tcgaccagca cgtcggcgcc atggaacgag
     8821 acaaccgtcg gtttcggcca gtggcggatc agcggcaaga gatggacggc gatgtggccg
     8881 aaatagatat gcagcaattg cgcgtcgttc gtggcgagaa gccgctcgat ctgcgttacc
     8941 tccccacgcg acatttgcca tggggcatcc tggagcgtgc gaaaccaaaa gcgccgcaga
     9001 aaatgcgtcg ccggttttcc cacgacgaag accggcgcaa acggaaagcg ctccgtttcc
     9061 tcgcgctttt gcgcgataac caccggccga aaacgctcga gcgccgtgat ctgccggtag
     9121 atatgcagca tctccggctt gagaaacgtc gcgcaataac aggcgacgac aggcggagag
     9181 cgttcgggca cgcctaacgt tcaacggcag cgcacgccgg gcaactcaat ctgagcgacc
     9241 agccgactca aaaactccag gcgaaaagcg agatgccctt cggtttgccc ggatcgttgg
     9301 gatcgtgcat gacatgctcc tgcgcgttgc catagcgcgc gggcgccgcg ggattgatca
     9361 actggatcgg ccggcccgtg cggatggcca cttgcaccgc tccttccgtc ggcacaaaag
     9421 gcgccgcggc gggcggcctc ggcttcggca gagtcgtcga caattccccg ttggatctgg
     9481 cctttgccgt ggtcgacaac ttaccctgca cctgagcctg cgcgaaggcc atcccggtgg
     9541 tcaacgcgag caacatgctg atgagcaatt ttttcttcat aatccttctc ccaagactgc
     9601 gagcgccgcg caatttcttg cccacgctct gacctccctc cacaattata gatcgatgga
     9661 ctacattgaa aaggccgcac gtgtgcttga tttggaaatt tccgagctcc agcggcttcg
     9721 tcaacggctc ggggaaagtt tcgcccgggc cgtcgaggta atcaagaaga cggtcgaagc
     9781 acgtggcaag atcattgtcc ttggggtcgg gaaatccggc cacatcggcg ggaaaatcgc
     9841 cgccaccctc accagcaccg gttcgcccgc ggtcgtcctc gattcgctca acgccctgca
     9901 cggggatctc ggcgtgatcg cggatggcga tgtcgtcctc gccctcagct acagcggcga
     9961 aaccgaggaa ctgctccgca tcctgccggc gatcgcgcgt tttcaagtcg gcgtgatcgc
    10021 tatctgcggc gcggccgatt cgaccctcgc gcagaattgc gacgtctttc tcgacgtcaa
    10081 cgtcgagcag gaggcgtgtc cgctcaattt ggcgccgacc tcgagcacga ccgtcatgct
    10141 cgcgctcggc gacgctctcg cgatggtcct gctcgaggcg cgcggttttc agaaggagga
    10201 tttcgcgaag tttcatcccg ccggcatggt cggccgcagc ctgctcctgc gcgtccacca
    10261 aatcatgcga ccgcgcagcg ccctcgcact cgttaggccg gagacgactg tccgcacggc
    10321 tctcgaatta atgacctcgc tccgcgccgg cgcggcggtc gtggcgggcg aagacgagca
    10381 gttgctcggc attttcaccc atggcgattt cgtgcggcat taccagaatg attcgcacgt
    10441 cggtgagcga ttcgtcgccg acctgatgac cctcaatccg gtggtggtgc ggcaggacaa
    10501 actcgcggtc gaggtgctca acctgctcga acgccatcgg atcgatgatc tcgtggtggt
    10561 ggacgagcgc ggcgtgccag tcggaatcgt cgattcgcag gacctgacga aactgaagtt
    10621 gctctgaggt ggaccctgga ctcgtcgcgg cgactccctc cgaagaacgc tcgcctgcgc
    10681 ctccgggttg atggtcaagc cgccggaggt tgctttaaga ccgcgctcct gctaaagctc
    10741 tcaactctca accaacgact ctcaactact tctcatggcc gccgctaacg atcttcgcaa
    10801 aggaatggca attaaataca atggcaacac cgccattgtc ctcgatgtgc accaccgcac
    10861 gccgggaaat ctgcgggcct tcgtccaggc gatcatccgt tacatcaaca ccggcaagag
    10921 cgccgatgtc cgtttcggtt cgaccgacaa ggtcgagctg gtcgatatct cgcggagcac
    10981 gctcgagttc agttacaagg acaacaccgg ctttcatttc atggatccga gcacctacga
    11041 aacgatcacg ttgcaggaaa gtctgctcgc cgaggccaag gattatctcg tcgagaatct
    11101 gccggtggag gtccttttca ccgagggccg gccggtgcag gtggaattgc cttcctcggt
    11161 acagttgaaa gtggtggaag caccggaggg tttgcgtggc gacactgcga gcaacgtcac
    11221 caagcccgcc ctgctcgaga ccggcaagac ggtcaatgtc ccgctcttta tcaacgaagg
    11281 cgaaacggtg aaaatcgata cccggacggg cgcctacatg ggccgcgcct aacgaatggc
    11341 ggcgcgccgc gtcacccggc acccggcacg tggccgcgtc aaggtcgcgc cctatgttgt
    11401 cccgacccgg cgcggcacgc cgcccgcggg atcgctcatg gtcccgacga agtgggtcaa
    11461 attcgtcgtc gccctttttc tcctccctct ttgcgccgtc ctcagccaga cgtttttcac
    11521 ctcgtttgcg cgcgcgaccg tcgcggagcg cctctgggcc ggtgaggagt tctggttctt
    11581 ttcgttaggt gccgttcttt ggctgatcgc ctttttcgga ttgccccggc cgatcgtgct
    11641 ctacgttttc ggccatgaac tgacccacgc gctttgggtc tggctgatgg gcggacgggt
    11701 cagccgtttc cgcgtcggcc gcgaaggcgg gcacatcctg accgacaaga acaactttct
    11761 catcgcgctc gccccttatt tttttccact ctacagcatt ttggtcctgg cgatttacgg
    11821 agcgctcagc ctgtgcgtga acctgcagcc ctacggccgg ttgctctacg cgctggtcgg
    11881 tgtgacctgg gcgtttcact tcaccttcac ctgctggatg atcccaaaaa atcagagcga
    11941 cctgcgcgat aacggcacga ttttttccct cgtcgtcatc tacctgatga atctgctcct
    12001 gctcagcgcg atgcttatta tcgcttcacc caatatcacc ttcgccgggt tcggcgccga
    12061 tatggtgcgg aacatcgcca acttttcgca cgcggtggtg aatctcgccg agcgcttcag
    12121 ccgcggcgcg tgacagcacc gcccgcactt tcccgtgtca gatcagaaca gcaaatggga
    12181 gccgggcgaa cgcctccgcc agcgcgaggg cggaattttt ttcctccttg gtttcaccgg
    12241 tgaagagatc gcggcccgcg gtaaagccgg ccggcaaatt caccgccgta tcgccccaag
    12301 cgggaccgat cgcgggaaat cccacgcgcg aagaaagccg cggcgcaagc acaatgatcg
    12361 agtggccttc gtgttctcgc gtaaacgcaa tgcaacaatc ggcgcgactg cccgttaggc
    12421 tgagaggcgt gtagcggcca tcgcgaaaca gcgccgcatg gtcccggcgg aaagtgagca
    12481 ggcgctgggt gaggaagagc ttgatccgtc cgtccgtcca gttgcctaac aaatcctccg
    12541 gcgtcgcgcc cgccagcgag tcgagcatgg cctggcgccg cgcgtaatcc accggccgcc
    12601 gattatccgg atcgaccaga ctaaaatccc aaatctcatt cccctggtag atgtccggca
    12661 ccccgggcga ggtcagtttg agcaaggttt gcgcgagcgc gttgatcgct cccgttcgcg
    12721 ccacttcctc cgcgagcggg atgaagcttt cgaggaaacg attgcgcggc gcgggatcaa
    12781 gaatttgcgc gacgaaatcc atcaccgcgg aatcccagag ttcgttaggc tgaacccagc
    12841 tggtcttgag cttggcctcc ttcagcgcct tcgccatgta ttgctggatg cgcgcgaggt
    12901 aggcgccgcg cgccgctgcg ctcagctcgc ggaacggctc cagcggccag gtgccgagga
    12961 gaatctggta gagcagatac tcctcattgg catccggcgc ctcgttctca tcgaccgatt
    13021 ttttccagcg cttgttcaga gtgcgccagc ggcctaacga acgccgccac tcctccggcc
    13081 gctccgcaat cgcgaccatc cgggcgcgca cgtcctcact ccgtttcgtg tcgtgcgtgg
    13141 aggtggcgag gagcgcggcc ggccagtgcg cctgccgctc ggcgttccgg cgatgaaagg
    13201 cttccacgtc caacccaaag cgctgcggtt cacccccgac ttcattgaga gccgcgaggc
    13261 gattgaaaat gtaaaacacc gtgtcctcga gaccctttgc catgatcggc ccggtgcttt
    13321 gctggaattt caggacgaaa tgcacgtggt cggcgcgctc ctcctcgctc aggttttcgg
    13381 ggaagcgaaa aagcaaaatg tcgcgcagga aattgaagac cgattcctcc agcgcgggat
    13441 tgcgccgctt ggccgctgcg accgcgcgct cgatcacggc gcgatcggca tcgttcaccg
    13501 gacggtccgg cgcgagatag gtccggtaaa ccgggaaaca ggcgatcgtc tcgcggacgg
    13561 cgagggtgag agtgtcgagg gtgtaatcgc gataccatcg atttttttcg gacaaccgat
    13621 cgaacatcgc gcccaaaaca ttgacgtcgt tggccagcga gaggcgcatg acgaggcgct
    13681 tcttcgcgta aacgaggtga ccgaattgca gggtgtgccc gataaatttg tgaaaagtgc
    13741 tggtgatggc gcgctccgcc gaaccgtcca ccagcacgcc gatcgcgtcg ttcatgaaat
    13801 cgtatccggt cgtcccgtgg accagccaat cgcttcgcaa ttgttccgtg ccgctcagga
    13861 ttttctcgac cacgagatag atcgcacgcc tggagggact cgccacggcg agtcccggag
    13921 acgcggaagc gcgtccctcg gcctgcagcc cttccgcgca acggccttgc aacttgctaa
    13981 tatattcctg cggcaaataa agcccgtcgg ggtgatcgat gcgcaagcca gtaaccgccc
    14041 ccgttccgac cagttcgagg agaagctgat gcgtcgcctc gaaaacttcc ggcacttcca
    14101 tccggatcgc ggccaggtcg ttgacgtcga aaaagcgccg gtagttgatt tcctccgcgg
    14161 ccacccgcca gaacgcgagg cggtaggact gctcgttgag catctcgtca aggccgtcaa
    14221 aactgcgcgc gtcgcccggc tggccgttga tgatcgtgag agctttttcg atcgccgcct
    14281 gcacttccgg cgcttcctgg cagcgtcgct ccagccggcg cttgatgatt tccttttccc
    14341 gcgtccgttc cgcgattcgc tccggatcgg tctcggtccg gcgcgggaga tattcgagcg
    14401 cggtgaggat gctctgcagc tcggcataaa attcctccgc ctgatgcgcg ctcaactgtt
    14461 cgagcgcaat ctggagaacg tggcggtagg tgccgggggc gatcgggaat tcatgttcga
    14521 aataacgcag aaaaaatcgg ccgctctcga agtgcacaac cagctccccg cgctcgagca
    14581 cacgtccgta ttggtcgccg aggatcggga gcagcacctt gccgcgcaga ccggatttga
    14641 gcggatgcca atcgatgtcg aaatagggag cgtaaaaaga gctcggcccg ttctccagca
    14701 catcggccca ccagagattg cccggctcga cgattcccat gtggttcgga acgaaatcga
    14761 gcatctgtcc catcgcgcgc gcgtgcagct ccgcgaccca ggcgtcatac tccgcgcgcg
    14821 acccgatcgc gtcattgagc tggttgtgat cggtaatgtc gtagccgtgc aggctctccg
    14881 cgcgcgcctg gaaatagggg gacgcataac aatcgctgat cccgagcgcg tggagatagg
    14941 ggacgatctc gcgcgcctgc gcgaaggtga attcgcggtt gaactgaagg cggtaggtgg
    15001 aactgggaat gcgtggatgg tcgggattca agacccttga tcatagcgga agcaaacgtt
    15061 caacgtccaa cgttcaacga aagaccctct tgctcgagtc attcgatgtt ggacgctggc
    15121 ttcagctgcg aaggtctccc tacattttct cgggcactct tatcccgaga agatcgagtc
    15181 cccgctgcaa agtgcgtgcg gtcaattcgc ttaaagcgag ccgcgtcgcg cgcgcaggtt
    15241 cgtccgcctt cagcaccggg caggcctcgt agaaagcgtg gaaggcgttc gccagttcga
    15301 agagataatt cgccaggatg ttgggacgaa aatcatgcag cacctgcggc acactctcgg
    15361 caaattgagt gaggcgcttg gccaggttca gctccgccgg ttccaccagc gtgagttcgt
    15421 taggagcgga gaatgcttcg cccgccttgc ggaagatcga gcgaattcgc acataggcat
    15481 tctgtaggta gggcgcagtg ttcccttgca gggaaagcat ccgatcccag gaaaaaatat
    15541 aatccgtcat tcgatactgc gagagatcgg catatttgac cgcgccgagg ccgatcgtct
    15601 gcgcgatcgc gttcttctcc gcctcggcga ggtcgggatt cttttcctcc acgattttgc
    15661 gcgcgcgggc gacggcttcg tcgagcactt cgcataacgg cacgttgtcg cccgagcgcg
    15721 ttttcatcag cttacggtcg gccccgagga tgctgccaaa ggtaatgtgg cgaaaatccg
    15781 cggtgtaacc ttcgcgccgc gcaatctcaa aaatctgttt gaaatgcaac gtctgcggcg
    15841 cacccacgac ataccagacc gcgtccgcct ttaagtcgtt gatgcggtaa tcgacggtgg
    15901 cgatgtcggt ggtggcgtaa ttgaagccgc cgtcgctttt ccgaataatg cacggccgct
    15961 cggcgagctc gggaatgtcg cggaagaaaa cgcagaccgc gccttcgctg atttccgcga
    16021 gaccggattt gagcagccga tccaccacgg ccgacaggcg atcgttgtaa aaactctccc
    16081 cgcgctggag gtcgtaatga atatcgagga tccggtatgt agcttcgaat tcctgtcgcg
    16141 aaagagcgac gcattcgttc cagatacgca gattttcctc gtcaccggcc tgcaacctga
    16201 cgagttcatt gcggcaggca tcacggaccg ctgggtcgct tttcgtcagt tcgtgggccg
    16261 tcttgtaaat ccggaccagc tccccgatcg gatcgcgcgc cagcgccgcc cgatcgagca
    16321 gatttttcca gccgtaaatc accatcccaa actgcgtgcc ccagtcgccg atgtggttgt
    16381 cgcggatcac gtcgtgcccg agaaattgcg cgatccgggc cagcgcgtcg ccgaggacgg
    16441 tgctgcggat atgcccgatg tgcatcggct tggcaacatt cggcgagccg aagtcgatca
    16501 cgatacgatg cggcttttcc gcgagcggaa caccgaggcg ttcgtcctgc aagagctggg
    16561 ccgctttcgc cgcgacggcc gccggctgaa gcgcaaagtt gatgaagccc gcccccgcga
    16621 ccgtcggcgg ctcgcaccat tccgcgacct cgagccgatc gatgattttt tgcgcgaggg
    16681 ctctcgggtt ttcgccccgt tgtttggcga ggacgagcgc gacgctggtc tggtagtcgc
    16741 caaaacgggc gtcggtcgcg ggcaccactt cgcccttggc aggcaatccg gaggcggcca
    16801 gcgcgcgcaa caattctcgc gtcaataaag tctggaaagt ttccatgaac gaatgcgacg
    16861 aacttaacag gcgactgaag cgtgtcatcc cgagcgctgc cgagggatcc cgcggcactg
    16921 cctgaaacgg ctgccagaag agttttgcag ccgtggcgcc atcttcacgt tgcctcccgg
    16981 gatctctcga ctgcgctcga gatgacggct cgcttgccgc cgttcgctac ttctgggagc
    17041 gctcctggaa aatcgccaaa atctcttcct tcgtcccggc gctcgcgagg ctctcgcgca
    17101 cggagcggtc attcaagaac ttggcgatcg acgcgagcgt gcgcagatgg gtctggaatt
    17161 gattcttcgg cacgataaaa agaacgacaa atttcaccgg ggcgttgtcg agggcatcaa
    17221 attcaattcc ctttgacgag cgtccgaaac ccgcgacgac ttcggcgacg gcctcggagg
    17281 aggcatgggg aatggcgatg ccaaagccga ttccggtgct catcgtttct tcgcgctgtt
    17341 tcaaggaagc gagaatgctt tcgcggttag ccggatcgat tttgccctgg cctacaagca
    17401 gatcgatcaa ctcgacgatc gccggccagc gctcggtcgc tttcatctcc ggaatgatct
    17461 gctcggaagt tagaaggctg gccagagaca tgcggagttt tccgagtgga gcgaaagctt
    17521 agcgtccgct cttgggttga caagcgcgaa tggcggcaaa ggaaccatag ttctgggcgt
    17581 cccaaacatc gcgctaatct ttcagccggt aagtgagctg ccgagagagc agccaacgca
    17641 ccccaaacat gtcgatggtg gcgcgcaccg cgcggttgcc gagcccgtat ttgctctggc
    17701 caaatttgcg cgcgcgatgg ttgaccggca cctcgaccaa ccggtagccg gcgcctttga
    17761 cgagcgcggg aatgaagcga tgcatgccct tgaagggcac gagcgtgcgc acgcattcac
    17821 ggcgcattgc cttgagcgtg cagccggtat cgcgcacccc gtctttcgtg aaccggctgc
    17881 ggacgaagtt ggcgatccag ctcgtcaggc gtttgaccag cgtgtctttg cgttgcgcgc
    17941 gatagccgca gaccaagtct gccccgcgct cgatctcggc gagcaggcgc ggaatgtcgg
    18001 ccgggtcatt ttgcaaatcg ccgtcgatca ggacgacggt cgcgccgcgg gccgcgttgg
    18061 ccccggcata aagcgccgcg ctctggcccg cattctgcgc aaagcggagc acgcgaaccg
    18121 ccgagccact cgttaggcga gcgagcgttc catcggtcga accgtcgtcg acgaagatga
    18181 tctcgtgctc gagtccggcg agcgcggccg ccaactccgc ctgcaaaatc ggcacgttct
    18241 cttcctcgtt gaagagcggc acgacctccg aaacctccgg gtgcgatggc ccactgctca
    18301 taggggcatc gtccggtaat cgtaacaggc gtaaacctgg atcgtgcgca gcagccggcc
    18361 gcccgtgcgc acttcgatcg tggcgacccg ctccatgcgc tcgaaggcgc cgcggatatt
    18421 gcggggcgcg cgctctggtt ttccctgctc aagatagagc gccgtgcggc cgacaaagac
    18481 attttcgccc tgctcctcgg tgtaaacttc gttcgcatca cgcgccgcgt tcggcggcag
    18541 gggcacaaac tcatcgtaac gcggccagaa ggaaaattgg ttcaccatgt cctgcgattc
    18601 agggaggtag accggcgggt gacccggccc ctcgacgcgt ttatcgggca ggtagaagga
    18661 aagctcggaa gcacgatcgc gtgcgtcggc gatcagaaaa acaggcttgc cggtctgctt
    18721 ttcgaagtcg cttctcacct ggttgacggc ttcggcgaac gaacgccaac cgcgtaatcg
    18781 atcgctcgga tcgcgtcctc gaaaatcaag cccgcgatta ttcaccagca aagcgacgag
    18841 tagcgcgctc atcaccaggc cgagaacgac cgcgactctg gtccaatttt gggcggcgac
    18901 gcgcgccgcg gaacgctcgc gccaatagga aatcgcaaga atgccgaggc tgaggaaagc
    18961 gagaccgtcc cagttcggcg cggcggcctt gttcagtgaa agaatcgtgt aaaacaggaa
    19021 gaccggcagc ccaaaccaaa ggagataaag caccttgaaa ttttgccggg cgcggcgcca
    19081 gctcgcgacc accgcccaaa cgagcccgag aaaaagtaac ggcgaatagg tgacgaaatg
    19141 ttccccgaga aaggaaagca gctcgagagg atgaaagccg ggcgcgtgat tgaggctgcc
    19201 ccgcgccttg agatgcgcga gcgtgatcca ggcgtgctgg gaattccaaa cgagcggcgg
    19261 aagggtgcag agcgcgcaga caaagaggag cagataaaga ctcggccgca caaattcgcg
    19321 gcgcagatcc cgggtgagaa ggaggaccag caaaatcgag atcaactcga gcgcgttggt
    19381 gaatttgcag agaaagccaa ggccgatgag caacccggtg aacggccacc accaggaaaa
    19441 cctggggctc cgctcaaggg cgagccagaa ggtgtaaaac gccgctaccc agaagaaaat
    19501 cgagagcgga tcgatcgtca ggacgaggct gccgagattg aaaatggggg tgatactaag
    19561 cgcgactacg gtccagaagc cggcgagcgc gctaaacaag cgggacgcga aataatacag
    19621 cagcaggctc gtgccggcgg cgaaaagcgg gctccagaag cggaccccga attcgttgtc
    19681 gccaaagagc gccgtgctcg accggatgac caaggccacg ccgggacctt tgctgaaata
    19741 agagagcgcc ggccgctgcc cccacatcca gtaataagcc tcgtcgggcg agagttgggt
    19801 ggcgttcagg agcgcgaggc gaaccaccgt gagcacgaac agaaaaatcc acacagcgcg
    19861 cgtcgtgctc atgagatgga gtcacccacc aggcggggat gcctgacgaa aaactgcggt
    19921 gcccagcgcg cggcggcgcg acgataaacc gtctcgagga gcgcgaggag gagcgcggtt
    19981 tcgccggccg ccaggaaaaa agtcgcgagc acgtcgctcg gccagtgcgc gccgagataa
    20041 atccgcgacc aggcgatggc cgcagccaac aagaaataaa gccatcccca gcgccggaag
    20101 aagaaggtga ggataagggc aacgctcaca ttgttgacca catgaccgga gggaaacgac
    20161 ggacccgagc ggtcgcgatc gcgggcgtcg gaatagcgaa tctccacttt tttgaagagg
    20221 gtcagaaatt ccgggcgtgc cttggccaac tccaccatcc gcacacgctc gacctgtttc
    20281 ggacggcgac gatcgaccag tttcttcaaa cctgaggtca ggctgccatt tacgaggagg
    20341 acaaagccga tgcagaaaac cagggcgcgc cccttgaatc caccgaagat taacgcagac
    20401 aacgcgagca ggacgaacaa cggtttccag atctcgacgt tgctgatcgc cgccatgaac
    20461 aaatcgagcg ccggatgcgt ccattgctcg ttgatgagat gaaacaaaat ttgatccatg
    20521 ccgaagcttt aggtttttag cagcgaccgg acgcgccagc ctcacttaaa gcttaaaacc
    20581 caaaacttaa agctctcctc atgcgcaaac tgatcattct cgttgccgga gccgtcctcg
    20641 cggggatggc ggctcacgtt cccgccgaac cgccttccgc accggaaatt ctcgaccagg
    20701 tgcggctcca acagtcgcag cagcaactcg atctgcaggg gcaactgcgc accggcgcga
    20761 cggtgatccc gtttcgcatg acccagaccg gtccggtgat tcgctacacc ttcgcgaatc
    20821 cgcccgaagt gctccaactt cgcctcggcg aaaagaattc gcggctcgat ttggtgacag
    20881 agagcagcac gaggaaattt tccgcctctc ggctcgacga aaaaatcggc ggcaccggcg
    20941 tgacctacgg cgatctcgcg ctgaagtttc tttattggcc aaacgcgcag gtcatcggcg
    21001 ccgataacat tcgcacccgt gattgctgga agctgcggtt gccggggccg gggaaggacg
    21061 cacaatattc cagcgtcctg ctctgggtgg acaaggagag cggcgcaatc atgcagctgg
    21121 aaggttacga ccaaacgggc cggctcgcga agcgcttcga agtggtctcc gcacagaaga
    21181 tcgagggtcg ctggttcctg aaacaaatgc ggatcgagga attgcagccg gggacgacca
    21241 aggtgctcgt gcgcacctat ctcgagatca agaaaccggg ttgagcgccc cgttgtaggg
    21301 caggctggag atgcgaagca tgtccctccg gtgtggggcg cgcttccgcg tgtcccagga
    21361 ccggcgatgc ggcggcgggt tgagtgaagc gcaaaaccgc gtagtagtgg gcggatgttt
    21421 tccgcacgtt tttccc
//
LOCUS       DATMNS010000055         3447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3615778, whole genome shotgun sequence.
ACCESSION   DATMNS010000055 DATMNS010000000
VERSION     DATMNS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3447
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3615778"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1224)
                     /locus_tag="VNW28_02185"
     CDS             complement(1..1224)
                     /locus_tag="VNW28_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXA08759.1"
                     /translation="MMTFSYQARDGAGNIVNGIQDAINEDNAVTSLMSRGLMVLSLQQ
                     KAAASRQKKKVWSVKETDLVLFTRQLATMIEAGISLVQALTALYEQCDAKKQKNLRNV
                     ISDVTARVQGGETFHESIAKHPRVFNRLFVSMVKAGEHGGLLSEILDRLAGFLEASAR
                     LRKKIKSAMTYPVIVISIAFLITTFLIVRVVPVFGEIFKDFGAKLPAPTQFLIDLSDF
                     IRANWYFMILGIGGTIFGTRAFLRSTRGRQLSDRWKLKLPVFGPLVHKICMSRFARTF
                     AQLIRSGVPILEVLEIVGGSSGNHVVEESIKGVSADVEKGDNLSVALSKKKIFPPMML
                     RMVAAGEATGRIDTMLEKMADFWDEEIEAMLDAMTSLIEPLLIVFLGVIVGGIVIAMF
                     LPIFKLNEVVSQSKS"
     gene            1299..1916
                     /locus_tag="VNW28_02190"
     CDS             1299..1916
                     /locus_tag="VNW28_02190"
                     /inference="COORDINATES: protein motif:HMM:NF025948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08760.1"
                     /translation="MITAAEVCRLTDSLWLWQAYDPSVKSDLFSSAARIGDRLLLIDP
                     IPLAAPALEELTLDRGVTAVLVTNLNHPRAAAAFARQFSAPIYAAEPVTREFGAANTV
                     TLAAGQQVAPGMTVIAIEGAAPGELAFHFAVDGGTTVIGDALIHLEPYGFALLPPKYC
                     SSQKEMRRSLRQLLDRPFERLLFAHGTPILSAARARLEALLAADH"
     gene            1925..2776
                     /locus_tag="VNW28_02195"
     CDS             1925..2776
                     /locus_tag="VNW28_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HXA08761.1"
                     /translation="MKPTESGLTRFLRLIRFSHTIFALPFALGALLVATRGRPSVRLL
                     TLVLVCMVCARTAAMLFNRIADWTLDQRNPRTETRHQLISRPVALALLVVSSAAFVTA
                     AAGINRTTMFLSPLALGIIFFYSLTKRFTAASHFFLGLALAVAPAGAWIAATGRLALA
                     PLLLGAGVVGWVAGFDLIYATQDLEFDRREGLRSLVVRLGLVRSLRLAQFLHLGMLLA
                     LLAFGFAASLGGVYFWGMPLVAAALVYEHRSAARLDIAGINRAFFESNAFVSAVFLVS
                     VAAALAR"
     gene            complement(2798..3226)
                     /locus_tag="VNW28_02200"
     CDS             complement(2798..3226)
                     /locus_tag="VNW28_02200"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase"
                     /protein_id="HXA08762.1"
                     /translation="MKIALGSDHAGFRYKEKIKELLISLGNEVEDFGTSSEEPVDYPK
                     FIRPAAEAVARGECDRGIVLGGSGNGEAIAANKVRGVRCALCWNEETARLSRQHNDAN
                     VLSLGERMIPEDLALRIVRVWLTTDFEGGRHIQRIAQLEP"
BASE COUNT          607 a         1091 c         1021 g          728 t
ORIGIN      
        1 ctagcttttg ctctgcgaaa ctacttcgtt cagcttgaag atcggcagga acatcgcaat
       61 cacgatgcca cccacgatca ccccgaggaa cacgatcagg agcggctcga tcaacgaggt
      121 catcgcatca agcatcgctt caatttcttc gtcccaaaaa tcggccatct tctcgagcat
      181 ggtgtcgatg cggcccgtcg cttcgccggc cgcgaccatg cgcagcatca tcggcggaaa
      241 aattttcttc ttcgaaagcg ccaccgagag gttgtcgcct ttctcgacgt cagcactgac
      301 gcctttgata ctctcttcga cgacatggtt gccggaagaa ccgcccacga tctcgagcac
      361 ttcgagaatt ggaacgccgc tccggatgag ctgggcaaac gtgcgggcaa agcgcgacat
      421 gcagatcttg tgcacgagcg gcccgaagac cggcagcttg agcttccagc gatcgctcaa
      481 ttggcggccg cgggtcgagc gcaagaaagc gcgggtcccg aagatcgtgc caccgatgcc
      541 gaggatcatg aagtaccagt tcgcccggat gaagtcactc aggtcgatca ggaactgcgt
      601 cggagcgggc agcttcgccc cgaaatcctt gaaaatttcg ccgaagaccg ggacgacccg
      661 gacgataagg aaagtggtaa tcaagaacgc gatcgaaata acgatgaccg gataggtcat
      721 ggcagacttg atcttcttgc gcaggcgagc actcgcttcg aggaagccgg ccagccggtc
      781 gagaatctcc gaaagcaaac cgccgtgttc gccggctttg accatgctga cgaagagacg
      841 attgaagacc cgcggatgct tcgcgatcga ctcgtgaaaa gtctcgccgc cctggacgcg
      901 cgcggttacg tcgctaatga cgttgcgcag gtttttctgc ttcttcgcat cgcactgctc
      961 gtagagggcg gtcaacgcct ggacgaggga gatgcccgct tcgatcatgg tcgcgagctg
     1021 gcgggtgaag agaaccagat cggtctcttt gaccgaccag actttctttt tctggcggct
     1081 ggccgcggcc ttttgttgga gcgaaagaac catgagcccg cggctcatga ggcttgtgac
     1141 agcgttgtcc tcgttgatgg cgtcttgaat accgtttacg atattcccgg cgccgtcgcg
     1201 agcttgatag gagaaggtca tcatatgatt gttctagtaa ttacactgac tatagcaaat
     1261 aggatgccac gctccccggc tcctcgcaca aattgcccat gattactgcc gccgaagtct
     1321 gtcgcctgac cgattccctc tggctttggc aggcttacga cccctctgtg aaatccgatc
     1381 tcttctccag cgccgcccgc atcggagatc gcctgctcct gatcgatccg atcccgcttg
     1441 ccgcgccggc cctggaagag ctgacgttag atcgaggcgt taccgccgtg ctggtaacca
     1501 atttgaacca tccccgggcg gcggccgcgt tcgcccggca gttcagtgcc ccgatctacg
     1561 ctgccgagcc agtgactcgc gaattcgggg ccgccaatac ggttacccta gcggccggcc
     1621 agcaggtcgc cccagggatg actgtgatcg cgatcgaagg cgcggcgccc ggcgaattgg
     1681 cattccattt cgccgtcgac ggtggcacga cggtaatcgg cgacgctcta atccatctgg
     1741 agccgtatgg gttcgccctc ctgccgccaa aatactgttc cagccaaaaa gagatgcggc
     1801 gctcgcttcg gcagttgctc gatcggccat ttgagcgcct gctcttcgct cacggaacgc
     1861 caattctctc ggccgcgcgg gcgcggctcg aagcgcttct cgccgccgac cattgaccat
     1921 cgaaatgaag cctacagaaa gcggcttgac gcgtttcctg cgcctgatcc gattttcgca
     1981 cacgatcttc gcgttgccgt tcgcgctcgg ggcgctcctt gtcgcgaccc ggggccggcc
     2041 ctcggtgcgg ctcctcacgc tcgtcctggt ctgcatggtc tgcgcccgga ccgccgcgat
     2101 gctcttcaac cgcatcgccg attggacgct cgaccagcgg aacccgcgca ccgagacccg
     2161 gcatcagctc atctcgcggc cggtcgcgct cgcccttctc gtagtcagct cggctgcgtt
     2221 cgtcacggcc gcggcgggga tcaatcgcac caccatgttc ctctcgcccc tcgcgctcgg
     2281 tatcattttt ttttactcgc tgacgaagcg gttcacggcg gcgagccact tttttctagg
     2341 gctggctctg gctgtcgcgc cggcgggcgc ctggatcgcg gcgacgggcc ggctggcact
     2401 ggctcccttg ctgcttggcg ccggcgtggt cggttgggtg gcggggttcg atttgatcta
     2461 cgccacccag gatctggagt tcgatcggcg cgagggattg cgctcactcg tcgtgcggct
     2521 cggcctcgtc cgttcactgc gcctcgcgca atttcttcac ctcggcatgt tgctcgcgtt
     2581 gctcgctttc ggcttcgccg cctcgttagg cggcgtctat ttttggggca tgccgttggt
     2641 cgccgcggcg ctggtttacg aacaccgcag tgcggcacgg ctcgatatcg ccggaatcaa
     2701 ccgcgccttt ttcgaaagca acgccttcgt cagcgcggtc ttcctcgtga gtgttgcggc
     2761 cgcgctcgcc cggtagaggc ttcggtctgc ggagcgccta cggctcaagc tgggcaatgc
     2821 gctgaatgtg acggccaccc tcgaaatcgg ttgtcagcca gacgcgcaca atccgcagcg
     2881 ccagatcctc cgggatcatc cgctcaccga gcgagagaac gttggcgtcg ttatgctggc
     2941 gcgaaaggcg cgcagtctct tcattccagc agagcgcgca acggaccccg cgcaccttgt
     3001 tggccgcgat cgcttcgccg ttgccggagc cgccgagcac gatcccgcga tcgcattcac
     3061 cgcgggccac cgcctcggcc gcgggccgga taaatttggg ataatcgacc ggctcttcgc
     3121 tcgaggtgcc gaagtcctcc acttcgttcc ctaacgagat gagcagttcc ttgatttttt
     3181 ccttgtaacg aaagccggcg tgatcggagc cgagagcgat tttcatttcg tcgttttata
     3241 ggaacgccgc ggccctgtcg aggcttcacc aaggtgggag gggcctctgc gccccgattg
     3301 gcggcgacgc gggatctgat cggggcgcag aggccctacc cacattctag gttcggggga
     3361 gcgcggcgag ctgttcccaa agtttgcgtt cggtctcgct caactttttc ggaatggcaa
     3421 tctgcgtgac cacgtaaaga ttaccgc
//
LOCUS       DATMNS010000056         5854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2575499, whole genome shotgun sequence.
ACCESSION   DATMNS010000056 DATMNS010000000
VERSION     DATMNS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2575499"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1043)
                     /locus_tag="VNW28_02205"
     CDS             complement(<1..1043)
                     /locus_tag="VNW28_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXA08763.1"
                     /translation="MDPTVTESERNQAAFYPVAGPANRTVLVILFAISFSHLLNDTIQ
                     SLIPAIYPLLKTSFHLSFVQVGLITLAFQMTASILQPFVGWYTDRRPKPYSLAWGMGL
                     TLGGLVLLSHATHYAMVVGAAALVGLGSSIFHPESSRVAHLASGGRHGFAQSVFQVGG
                     NAGSSLGPLLAALIVVPHGQASVLWFAVVALLGIGILSGVGRWYGEHLAEARSRPTPG
                     EQTAHPLSRRVWFALSVLGVLIFSKYFYLASLTNYYTFYLIHKFHLSVQGAQLHLFLF
                     LFAVAVGTILGGPVGDRYGRKLVIWVSILGVAPFTLALPHANLFWTGVLTVIIGLILA
                     SAFSAILVYAQEL"
     gene            1141..1503
                     /gene="queD"
                     /locus_tag="VNW28_02210"
     CDS             1141..1503
                     /gene="queD"
                     /locus_tag="VNW28_02210"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HXA08764.1"
                     /translation="MRARLTKDFTFEAAQTLPAAPEGHKCRKLHGHSFKVEVTVEGEV
                     DRVSGWVYDHAEIGAAMKPLIAALDHSYLNDIAGLENPTIENMAAWFWEKIAPQCPGL
                     SEIVIHETPTARCVYRGL"
     gene            complement(1745..2371)
                     /locus_tag="VNW28_02215"
     CDS             complement(1745..2371)
                     /locus_tag="VNW28_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HXA08765.1"
                     /translation="MPNLASWMRRRDEKWFRPILATRPGIKVWNAARRAVALEEMDAL
                     LLTGGNDIAPEYLRQPVPDPSVLEKSDLPRDKWEFVAVQNALDRGLPILAICKGLQLL
                     NVALGGTLQLDIEGHNLPAQKSHDVQPLRSDATARHRFAKVNSCHHQAVDRLAEGCVV
                     ESWCAADDVIEQFRLRDRPFGIAVQYHPERGTLYGPLFEDFFDRINGS"
     gene            2509..2676
                     /locus_tag="VNW28_02220"
     CDS             2509..2676
                     /locus_tag="VNW28_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08766.1"
                     /translation="MATKHPKAKRKSPARKVRDLAARKDPKGGAQKKEGPDDLTGMSN
                     RPLTANTRRSA"
     gene            2904..3857
                     /locus_tag="VNW28_02225"
     CDS             2904..3857
                     /locus_tag="VNW28_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA08767.1"
                     /translation="MSVAEIAAVALTASDLTHRYGDRIALDRVSLRIAPREIFGLLGP
                     NGGGKTTLFRILSTLVRPSDGSASILGLDLLRETAALRSRIGVVFQAPSLDKKLRVRE
                     NLAHQGHLYGLSGTPLRERIDHLLLEFNLRDRARDLVETLSGGLQRRVEIAKSLLHRP
                     ELLLLDEPSTGLDPGARIDLWQTLYRLRDQQDVTVLLTTHLMEEAERCDRVAIIDRGR
                     IVALNTPDALRAEIGGDVISARTKDATSLGERIATTLEVEVSVLNDEVRIEHSDGPAF
                     ITRLLETFPGEIDSVTLAKPTLEDVFIAKTGRRLSEEQADA"
     gene            complement(3937..5664)
                     /locus_tag="VNW28_02230"
     CDS             complement(3937..5664)
                     /locus_tag="VNW28_02230"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXA08768.1"
                     /translation="MKFLALLSLAGLALAGAARATPADEHFETTAKAYIEQYLQRNPE
                     EATNLGDHRFDARLTDYSPHAFAEARAAAHDFRTRLGKIDRAELTGANKIDAQILQDA
                     IDYELFQLDELKEWESNPLVYNESLANSLYLLVARDFAPAADRIANLRKRMEALPAVI
                     VQAKANLQRPPEIDTQTAIEQTQGAISLVRQGFAPLLDQAPELKKEIAPLQEKTGAVL
                     EDYKKWLQSDLLPRSVGDFRLGEEKYRKKLHFALASDLPMEEVMKRAEADLASTQAAI
                     YETALPLYKKYFPEADPAALVDKHKVTAAVLDKLAQEHPDNDTIVDYAKKVLAEATAF
                     VRAKNFVTVPNEPLDIIVMPEFKRGAGIAYCDSPGPLEKNGKTFFAIEPTPNGWPEER
                     KKSFFREYNNYMVHDLVVHEAMPGHYLQLMHSNQFKGPTLVRAMFQSGSMIEGWAVCM
                     EQTMAENGFGGPEVKIEQLKMRLRVIVNAIIDQRIHAGKMTEKEAIDLMEKEGFQQEG
                     EAVAKWKRARITSAQLSTYFVGVSEWLDLRARAQAKAGKDFDAKKFNDEALSFGSPPV
                     KYIREMMGL"
BASE COUNT         1133 a         1794 c         1849 g         1078 t
ORIGIN      
        1 atcaattcct gcgcgtaaac caggatcgcg gaaaaggcgg aagcgagaat gagaccgatg
       61 atgacggtga gcacgcccgt ccagaaaagg ttcgcgtgcg ggagggcgag ggtaaagggc
      121 gcgacgccga gaattgaaac ccagatgacc agcttgcgtc cataacggtc accgaccgga
      181 ccgccgagga tcgtgccgac ggcaacggca aagaggaaga gaaagaggtg caattgcgcc
      241 ccctggaccg agagatggaa tttgtgaatc aggtaaaacg tgtaataatt cgttaggctg
      301 gcgagataga aatacttcga aaagatcagc acgccgagga ccgaaagggc gaaccagacc
      361 cggcgcgaaa gaggatgggc cgtttgttcg ccgggtgtcg gccgcgacct ggcctcggcc
      421 aaatgttccc cgtaccaacg acccactccc gacagaatgc cgatgccgag gagcgcgacg
      481 accgcaaacc agaggacgct ggcctggccg tgcggcacca cgatcaaggc ggcgaggagc
      541 gggcctaacg aactgccggc gttcccgccg acctggaaga cggattgcgc aaaaccgtgc
      601 cggccgcccg aggcaagatg ggcaacgcgt gacgattcgg gatgaaagat cgacgaaccc
      661 aggccgacga gcgcggccgc cccgacgacc atcgcgtagt gcgtggcgtg ggagagaagc
      721 accaggccgc ccagcgtcag ccccatcccc caggcgaggg aatacggctt tggccggcgg
      781 tcggtatacc aaccgacgaa cggttgcagg atcgaagcgg tcatctggaa ggcgagcgtg
      841 atcaagccga cctgcacgaa gctgaggtgg aagctcgttt tgaggagcgg ataaatcgcg
      901 gggatgagcg attggatcgt gtcgttgagc aagtgcgaaa aactgatcgc gaagaggatc
      961 acgagcacgg tccgattggc cgggccggcg accgggtaga acgcggcctg gttgcgctcg
     1021 gattcggtca ccgtcgggtc catgacggag ggaagctagt caggcatcgg gcggacgcga
     1081 atcacgcagc ccgtggcacg ggatgacagg cgcactgagg agtgcgattt ttgcgccgcg
     1141 atgcgtgccc gcctcacgaa agactttacc ttcgaagccg cccagactct tcccgccgcg
     1201 cccgaaggcc ataagtgccg caagctgcat gggcacagct tcaaggtgga agtgacggtc
     1261 gagggcgaag tcgatcgagt gagcggctgg gtctacgatc atgccgagat cggcgcggcg
     1321 atgaagccgc tcatcgcggc gctcgatcac tcgtatctga acgacattgc cggcctggag
     1381 aacccgacga tcgagaacat ggcggcgtgg ttttgggaaa agatcgcgcc gcaatgcccg
     1441 gggctgagcg aaattgtgat ccacgagact ccgaccgcgc gctgtgtcta ccggggattg
     1501 tagggcggtt tgtgaccgcc gtagcctaac gaacacgcgg tgatcggcag ttgtagaccg
     1561 ccctacaact tgcgacaacc tattgccgcg ccgcatgcac cgtgccggtc tcgatcgccg
     1621 ctcggcgttg ctcgacgatc cggctcgcaa tcgtgttctg atgtggacat cggaaaccat
     1681 ggacaggaat acaggatgaa aaggatttgg gggaaggcgg gaacgggaat tcccaaaaat
     1741 ccagctaaga cccgttaatc ctgtcgaaga aatcctcgaa gagcggcccg taaagtgtcc
     1801 cgcgttcggg atgatattgc accgcgatcc cgaacggccg gtcgcgcaga cgaaactgtt
     1861 cgatcacgtc atccgccgcg caccacgatt ccaccacgca tccctccgcc agccggtcga
     1921 ccgcctggtg atggcagctg ttcaccttcg caaagcgatg cctggcagtg gcgtcactgc
     1981 gcagcggttg cacgtcgtgc gacttttgcg cgggaagatt gtgtccctca atgtcgagct
     2041 gcaatgtgcc gccgagcgcg acattgagca gttgcagacc tttgcagatg gcgaggatcg
     2101 gcaacccgcg gtcgagtgcg ttttggaccg cgacaaactc ccatttgtcg cgcggcagat
     2161 cacttttttc caggaccgac ggatcgggca ccggctggcg cagatactcc ggcgcgatat
     2221 cgttaccacc cgtgagcagg agcgcatcca tctcctcgag cgcgacggcg cggcgcgcgg
     2281 cattccagac cttgatcccg ggacgggtcg cgaggatggg acggaaccac ttctcgtcgc
     2341 ggcggcgcat ccagctggca aggttaggca tcggcgaatt tgcgaccgct ggtgcgcggt
     2401 gtaaaggggc gcgccggggc gtcgtcttct tcctttccgc cgggcgaagt tgtcaggagg
     2461 ctgcaaaacg tcgaagatcc tcgcaggctc ttaagaaaag aaatccatat ggctaccaaa
     2521 catccgaaag cgaaacgtaa atcgccggct cgcaaggtgc gcgacctggc ggcgcgcaaa
     2581 gatccaaagg gcggggcgca gaagaaagag ggtccggacg atttgacggg catgagcaac
     2641 cgcccactca ccgccaacac tcgacggtcg gcgtaacctc gtcccgcggc cgcgggattt
     2701 tcggcgccgt ggcggtatct gtttaggttg cggaaaacgg tcgcgacaga cgcagactat
     2761 ggcccgcccc gaccagccgg gttcaccgcc ccggcgacaa actcgcaggc cccaccttcc
     2821 tcgccccgag ccgttcggcc aggcgccgac cggagcgcgc gagagcgcgt gcgctgcccg
     2881 ggagttcccg ctatcatctg acgatgtccg ttgccgaaat tgccgcggtt gcgctcacgg
     2941 cgagcgacct gacccatcgt tatggcgatc gcatcgcact cgatcgcgtc tcgctccgga
     3001 tcgcgccgcg cgagatcttc ggcctgctcg ggccgaacgg cggcggcaag acaaccctct
     3061 ttcgcatcct ctccacgctc gttaggccga gcgatggctc ggccagcatt ctcggccttg
     3121 atctcctgcg cgaaaccgcc gcgttgcgca gccggatcgg cgtcgtcttc caggcgccga
     3181 gcctcgacaa gaaactgcgg gtccgcgaaa acctcgcgca tcagggacat ctctacggcc
     3241 tttccgggac cccgttgcgc gagcgcatcg atcatctcct gctcgaattc aacctgcgcg
     3301 atcgcgcgcg cgatctggtc gagacgcttt ccggcggttt gcagcggcgg gtcgagattg
     3361 cgaaaagttt gctacatcgg ccggagcttt tgttgctcga cgagccctct accggtctcg
     3421 atccaggggc gcggatcgat ctttggcaga cgctctaccg cctgcgcgat cagcaggacg
     3481 tgacggtcct tctcaccacg cacctgatgg aggaagcgga gcgctgcgat cgggtcgcga
     3541 ttatcgatcg cgggagaatt gtcgccttga atacgccgga tgcgctccgg gccgagatcg
     3601 gcggcgacgt catcagcgcg cggaccaagg acgcgacgtc gttaggcgaa cggatcgcca
     3661 ccactttgga agtggaagtc agcgtcctga acgatgaagt gcggatcgag cattcggatg
     3721 gtcccgcttt catcacgcgc ctgttggaga cgtttcccgg cgagatcgat tccgtgacgc
     3781 tggccaagcc gacgctggaa gatgttttta tcgcgaaaac cgggcggcgt ctgagcgaag
     3841 aacaagcgga tgcctagcgc gtgcgggagg ggcctttgtg ccccgactgg gattgcagcg
     3901 cgtccgttga tcggggcata aagaggcctc ctaagtttac aaccccatca tttcgcggat
     3961 gtatttcacc ggcggacttc cgaaactcaa agcttcgtcg ttgaatttct tcgcgtcgaa
     4021 atccttcccc gctttggctt gcgcgcgggc tcgcaggtcg agccactcgc tcaccccgac
     4081 gaagtaagtc gataactgcg ccgaggtgat gcgcgcccgt ttccatttcg cgaccgcctc
     4141 gccttcctgc tggaaccctt ccttctccat cagatcgatc gcttccttct cggtcatttt
     4201 cccagcatga atgcgctggt cgatgatcgc gttgacgatc acgcgcagcc gcatcttcag
     4261 ttgctcgatc ttcacttccg gaccgccgaa gccgttctct gccatggtct gctccatgca
     4321 aaccgcccag ccctcgatca tgctcccgct ctggaacatc gcgcgcacca gggtcggccc
     4381 cttgaactgg tttgaatgca tcagctgcaa atagtgcccc ggcatcgctt cgtgcaccac
     4441 gaggtcgtgc accatgtagt tgttgtattc gcgaaagaac gatttcttcc gctcctccgg
     4501 ccagccgtta ggcgtgggtt cgatcgcgaa aaaggtcttg ccgttttttt ccaacggccc
     4561 cggtgaatcg cagtaggcga tgcccgcccc gcgcttgaac tccggcatca cgatgatgtc
     4621 gagcggctca ttcggcacgg tgacgaagtt cttcgcccgg acgaacgctg tcgcctcggc
     4681 cagcaccttc ttcgcgtagt ccacgatcgt gtcattgtcc ggatgctcct gcgccagttt
     4741 gtcgagcacc gcggcggtca ccttgtgctt gtcgaccagg gccgcggggt cggcttccgg
     4801 aaaatatttt ttgtaaagcg gcagcgcggt ctcgtagatc gccgcctggg tcgacgcaag
     4861 atcggcctcc gcgcgcttca tcacttcttc catcggcagg tcggaggcga gggcgaagtg
     4921 cagtttcttg cgatacttct cttcgcccag ccggaagtcg ccgacggagc gcggaaggag
     4981 atcgctctgc agccatttct tgtagtcctc gagcacggcc ccagtcttct cctggagcgg
     5041 cgcgatttcc ttttttagtt ccggcgcctg gtcgaggagg ggcgcgaacc cctggcggac
     5101 taacgagatc gcgccctgcg tctgctcgat cgcggtctgg gtgtcgatct ccggcgggcg
     5161 ctgcagattg gccttggcct gcacgatcac cgccgggagc gcctccatcc gcttgcgcag
     5221 gttggcgatc cgatccgcgg ccggcgcgaa atcgcgcgcc acaagcaggt agaggctgtt
     5281 agcgaggctc tcgttgtaaa ccagcgggtt cgattcccat tccttcagct catcgagctg
     5341 gaagagttcg taatcgatcg cgtcctgcag aatctgggcg tcgatcttgt tcgccccggt
     5401 cagctccgcg cgatcgatct tccccagccg ggtgcggaaa tcgtgcgccg ccgcgcgcgc
     5461 ctcggcgaaa gcgtgcggcg aataatctgt caggcgcgca tcgaatcgat gatcgcccag
     5521 gttcgtcgcc tcctcgggat tgcgttgcag atattgttcg atgtaagcct tggccgtcgt
     5581 ttcgaagtgt tcgtcggcag gcgtcgcgcg cgcggcgccg gcaagggcca acccggccag
     5641 ggaaagaagg gcgagaaatt tcataagcga aaacaggttg gcgcgaatcg cgcccggatg
     5701 cgagccgcaa ctcggacgaa tgctgcgcct agtcggggcg cagaggcccc tcccacgttt
     5761 tcgcctccgc cagtgcggcc tgctttttgc ctaaccaaag actgagcacg caccagagcg
     5821 cggcgagcgg cgcggcagca aacgagatgc cggt
//
LOCUS       DATMNS010000057         9785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_785300, whole genome shotgun sequence.
ACCESSION   DATMNS010000057 DATMNS010000000
VERSION     DATMNS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9785
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785300"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..4050
                     /locus_tag="VNW28_02235"
     CDS             100..4050
                     /locus_tag="VNW28_02235"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXA08769.1"
                     /translation="MVEKGDSAVQPRRSRSWIRRLLIALATIFLLLVVFHGPILRSVV
                     HSLAIHLAAKENLKLDFQIEGGILGEIELRNVHAAATGPAAVQSFDADLIRANYSLTN
                     LIFHGTADFLKDVEVRNVTAVLDPAKAAVPLPTTPSQPNEINLPVFFPDRLQVTSFNL
                     TIRGRPQDTVVRNFNLGLYPDREGVLRIDQLQIPDVHQWTDITATTTYANKNIFLRNL
                     TLDDGHHFQTVNIDASKAGGGRLRLQIAGSLGKGKIEGNVDLNPTKSSFNTSTKVSAS
                     GISLGELSEYFGRPAGALAGEVKDFKMDWRGTLDQPQTWNGTITAQIENVRQSGLELD
                     HVGLEVVAADGTATVREARADTGTNHVQLRGTVQLPETTEGFRRTPGNLQVAVDAPDL
                     KKLTAFFPQPVTGSLQANGTIRTDDSVARLELAVKGDLVGFDKAAAKTLTAKISATKK
                     LPAIGATEEPFYANLTSAISAELDEVRYDQFVVDGINAEVTSKDAAVSFEPVSVRRKA
                     NLLLVRGNYQLPPLGSTESRLTAQPVDLQLSFRAPQLADYWPDEAPNKVTGELQADGS
                     AQIRKGVANGQMNLSGQGIAAQKLLVKEFSAQVAIANNVAFLNDFTAILNKKDDVVAH
                     GSAQLQTPFHYSGSVTANLADLSTFEPLLRSDAPGGPGSATPPRAEGKARPLAGSLVL
                     DWQGEGDAATFRNTGNLKAKLENGRYAELKNLRATIDAKYTPQELNVPLIFLGSDTMD
                     FQASLQATGSTLEISKIQVDQGKAKFATAYAALPFTWSNIGRGRPLFPPDGKVLISFQ
                     SESLDLKKLFEDFGVPPPVSGSLDVKLEAKGPLDRLTASLDLRMQGLRATAGQSLEPA
                     TFDLGLRLQDNELKAAGKLQQPKVQPLEIEARLPLNLSQTIAQKKLDQNTPLSGQLRM
                     PRSSVNFVRQFVPALRVLDGSLALNVDVGGTIAQPALSGAADMNINLARFENATLPAL
                     TNFKALLSFRQNTLNFDRFGGDLAGGPFTLAGKITLPKLTEPMLDLSLKANSVLVARN
                     DNLTARVDADLKVQGPLQSATVSGNVLTTNSRFLKNIDIIPIGLPGRPAPQPPAATPI
                     LSIPTPPLRDWKFDLVVKSKDPFLIQGNLATGEAIIDLKLSGTGLRPGLQGEVRLAKF
                     EATLPFSTLTIEYGFLYFNPDDPLNPRIELHGTSLLRDYTVHVFVYGTALAPEAVFNS
                     EPPLPQEEIISLLATGVTREELTGSGNVLVGRAGLLLMKQLYRRVFKNGDESTTKSDS
                     LFNRLDVELGAIDPRTGQQTATAKFKVNDHVVLVGDIEVGGDFRGLVKYLIRFR"
     gene            4135..6297
                     /locus_tag="VNW28_02240"
     CDS             4135..6297
                     /locus_tag="VNW28_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXA08770.1"
                     /translation="MKWPASFLLFSCLTTDFALAQDAVQLPVQKQEAEEKRAVAQAQE
                     KVAQHANLEIRGNTAFDDKVLRSQLKEQIATIEKYGLTSARGDDAAFFLELFYKKHGY
                     AEVHVRYTIEDASRLQLDIAEGPLVHLGKIVFVGRQQLPEERLLNYALGPTRERYSKA
                     QSLLPFVSADLEEGASLVQRFYVSEGFVECTIEKPQDEYVRPDLVDVRIVVHEGHQYF
                     FGNINFVGPTIYGGEALRGQMLDLLQRPYTEARLEDIPRRLQAYYKTRGYYAVKADAF
                     GDPSLAVDGKVPVRVTIEPGAIYYFDGVTVKGTQKLRPSYLARRFRKFHGRPYSPEAI
                     DKRFRELMRTSLFNILQINPTPVDGNHLRLDINVEEAKTREFGFSIGYGSFSGAIVGA
                     SYANRDLFGYGRPVTTSVEYSERGYKGEVTYENPYLFDTDFAFKARLSALTYDYDGYS
                     KFELGSRFSLSRKITDQYEVGLVYGARHVEVTDATIDTALLGRTSYFISTIGFTQTLD
                     LRKNPFVAPRGLIFDNTFDLASSAIGSQIELIRGTARLSYYIPFASGPPALVGEDLTK
                     SAFQRWFERSLLAFGARSGIVYPLDNAGKSTALAIPIDERFFSGGGTTVRSFTERDLG
                     PHDAGGDPIGGEFFTIFNAEYTFPIYGEILGAVFVDAGNLLPDARSPGLNDMRYGVGL
                     GLRYNLPIGPVRVDYGFNPDPHANEASGAFNLSFGFAF"
     gene            complement(6371..6925)
                     /locus_tag="VNW28_02245"
     CDS             complement(6371..6925)
                     /locus_tag="VNW28_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08771.1"
                     /translation="MRRWLMVLGMLVAAGPAFALEDPELPGQSDSLPADIEPPLLLQN
                     LASDESTLDPRSTSAIENDLTRARQRAASADRLFRAGIIAKVEAEQRVLRVVQLEAML
                     AEARLEEARKVAAETGKPGNESEIAAIAEAARIATERRHRAELEAALRNLERQKKLLA
                     LGSGRKSDVNRAEEKLAELQRPAK"
     gene            7085..8218
                     /gene="hppD"
                     /locus_tag="VNW28_02250"
     CDS             7085..8218
                     /gene="hppD"
                     /locus_tag="VNW28_02250"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HXA08772.1"
                     /translation="METAIAAPQKTAAAQPDFLPLEGTDYVEFYVGNAKQAAHFYKTA
                     FGFQSLAYAGPETGQKDRVSYAIRQHKLTFVLTTPLRPGTPIADHISKHGDGVKYLAL
                     RVPDATSAWNETTKRGGKSYMQPTRSKDADGEVVMSGIHTYGDTVHLFIERKNYRGVF
                     MPGFRRWESGYNPPDTGLQYVDHCVGNVGWNQMNPWVKFYEDVMGFRNILSFDDKDIS
                     TEYSALMSKVMSNGNGFVKFPINEPAEGKKKSQVEEYLDFYDGEGVQHCALATKDIVK
                     TVTDLQRRGVEFLNIPTTYYDTLPERVGHIDEDLEPLKRLGILVDRDNEGYLLQIFTK
                     PVEDRPTLFFEIIQRKGAKSFGKGNFKALFEALEKEQELRGNL"
     gene            8235..9419
                     /locus_tag="VNW28_02255"
     CDS             8235..9419
                     /locus_tag="VNW28_02255"
                     /inference="COORDINATES: protein motif:HMM:NF042789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HXA08773.1"
                     /translation="MPYYQRLGQMPKKHHIWFHRNGAAPTYKNEGIAYEHVFTTEGFD
                     EAYSIVYHLRPPTRVRNVELIKHWAPKKVTDSPLRHHHLKTADIPRRGDLYTSRIPML
                     FNQDIIAYRARPAKTYDKFEYYKNGGADEVIFVFKGGGTLETVFGKLPYRAEDYIVIP
                     RGITYRLVPDNVQEEDYLILESAGPVRIPKRYLNHEGQIKMGAPYSERDLHGPKEIIT
                     IDKEEDVDVLVKDGSRFTRVTLAQHPFDAVGWDGYLYPFTFNAQDFEPITGTVHQPPP
                     IHQTFEVRGYVICTFAPRLLDTHPEAVKIPWVHDNVEADEVLFYVRGNFGSRRGIESG
                     SITLHPRGIPHGPHPGTIMASKDALRTEELAVMFDTAHTLELTEESLALDDPKYPLSW
                     LD"
     gene            9499..>9785
                     /locus_tag="VNW28_02260"
     CDS             9499..>9785
                     /locus_tag="VNW28_02260"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HXA08774.1"
                     /translation="MQETDFEISERLTSAPDRMGTDRLEEMERLFGDLLDAIGEDRTR
                     EGLRKTPDRAARAFEFITAGYRQSVEEAVNGAIFDSDASEIVMVKDIELYS"
BASE COUNT         2053 a         2942 c         2931 g         1859 t
ORIGIN      
        1 gaacgaaaac ggggggcaaa gtaagcccgg ggaacagtta gtccagagag gggcgatgcg
       61 cggttgcgat tgcaaggggc ggcaataggt taacgcctcg tggtcgaaaa aggagacagc
      121 gctgtccaac ctcgccgcag cagaagttgg atccgccggc tcctcatcgc actcgccacc
      181 attttcctgc tgctggtcgt gtttcatggg ccgatcttgc gctcggtggt ccattcgctg
      241 gccattcatc tggcggcgaa ggaaaatctg aaactcgatt tccagatcga gggcggcatc
      301 ctcggcgaga tcgagctgcg caacgtccac gccgcggcca ccggccccgc tgcagtgcaa
      361 tcattcgacg ccgatttgat tcgggccaat tacagcctga cgaatctcat ctttcacggc
      421 acggcggatt ttctgaagga cgtcgaggtg cggaatgtga ccgcggtgct cgatccggcg
      481 aaggctgccg tgcccctccc gacaacgccg tcccagccta acgagattaa tctgccggtg
      541 tttttccccg accgattgca ggtgaccagt ttcaacctga cgattcgcgg tcggccccag
      601 gacacggtgg tgcgcaattt caatctcggg ctttatcccg accgcgaagg cgtgttgcgg
      661 atcgaccagt tgcagattcc agatgtccac cagtggaccg acatcaccgc gacgaccacc
      721 tacgcgaaca agaacatatt tctgcgcaac ttgacgctcg acgacgggca tcatttccag
      781 acggtcaaca tcgacgcgtc caaggctggt ggcggtcggc tgaggttgca gatcgcgggt
      841 tcgttgggga aaggaaagat cgagggaaat gtcgatctca atccgacgaa atcatccttc
      901 aacacgagca cgaaggtgag cgcctccggc atttcgctgg gtgagctgag cgagtatttc
      961 ggccgcccgg cgggcgctct ggccggggag gtgaaggatt tcaaaatgga ttggcgcggc
     1021 actctggacc agccgcagac ctggaacgga acgatcacgg cccagatcga gaacgtccgg
     1081 caaagcggac tcgagctcga tcatgtaggg ctcgaggtgg tggcggcgga tggcacagcg
     1141 accgtgcgcg aggcgcgcgc cgacaccggc acgaatcatg tgcagttgcg cggcacggtg
     1201 caattgccgg agacgacgga gggttttcgc cgcacgccgg gaaatctgca ggtggcggtg
     1261 gacgcgcccg acctgaaaaa gttgacggct ttcttcccgc agccggtgac cggaagcctg
     1321 caagccaacg gcacgattag gaccgatgac tcggtcgcgc gcctggagct ggccgtgaag
     1381 ggcgacctgg tcgggttcga caaggcagcg gcgaagacgc tcacggcgaa gatttcggcg
     1441 acgaagaaac tgcccgccat cggagcgact gaagagccgt tttacgcgaa tctgacttcg
     1501 gcgatttccg ccgagttgga tgaggttcgt tacgatcaat tcgtcgtcga cgggatcaac
     1561 gcggaggtca caagcaagga tgcggcggtg tcgttcgagc ccgtgtccgt gcggcgcaag
     1621 gccaacctgc ttctcgttag gggaaattat cagctgcctc ccctcgggtc gacagaatct
     1681 cgcctcaccg cgcagccagt agatctgcag ctttcatttc gtgcgccgca actggcggat
     1741 tactggccgg atgaggcgcc gaacaaggtc acgggcgaat tgcaggcgga cggcagcgcg
     1801 cagatccgca aaggcgtggc gaacggacag atgaatcttt ccgggcaggg aatcgcagcg
     1861 cagaagcttc tggtgaagga gttcagcgcg caggtcgcga tcgcgaacaa tgtggcgttc
     1921 ctgaatgatt tcacagccat cctgaacaaa aaggatgatg tcgtggccca cggcagcgcg
     1981 cagttgcaga cgccatttca ttacagcggc agcgtgacgg cgaatctggc ggatctgtcg
     2041 accttcgagc cgttactgag gagcgacgcg ccgggagggc cggggtcagc gaccccgcct
     2101 agagcggagg gcaaggcgag accgcttgcg ggttcgttag tcttggactg gcagggggag
     2161 ggcgacgcgg cgacttttcg gaataccggc aatctcaagg cgaaactcga gaacggtcgt
     2221 tacgcggagt tgaaaaatct acgggcgaca attgacgcga aatacactcc gcaggaattg
     2281 aacgttccgc tcatttttct cgggagcgac acgatggatt tccaggcgag cctgcaagcc
     2341 acgggctcga cgctcgagat cagcaaaatt caggtcgacc aggggaaggc gaaattcgcc
     2401 accgcttacg ccgcgctgcc gtttacctgg agcaacatcg gccgcgggcg cccgcttttt
     2461 ccgcctgacg gcaaggtgct gatcagtttc cagtcggaaa gcctcgattt gaaaaaattg
     2521 ttcgaggatt tcggcgtgcc gccgccggtt tccggctccc tcgacgtgaa gctggaggca
     2581 aaggggccac tcgatcggtt gacggcgagc ctcgatctgc ggatgcaagg actgagggcc
     2641 acggcagggc aaagcctgga accggcgacg ttcgaccttg ggctccgctt gcaggataac
     2701 gagctaaaag ccgccggaaa actccagcaa ccgaaggtgc aaccgctgga aattgaggcc
     2761 cgtctgccgc tcaatctctc gcagacgatc gctcagaaaa agctcgatca gaacacgccc
     2821 ctcagcgggc aactgcggat gccgcgcagc tcggtcaatt tcgttaggca atttgtcccg
     2881 gcactccgcg tgctcgacgg cagccttgcc ctgaacgtgg atgtgggggg aacaatcgcc
     2941 cagccggccc tgagcggggc ggccgacatg aatattaatc tggcgcgctt cgagaatgcg
     3001 actcttcctg ccctaacgaa tttcaaggcg cttctcagct tccgccagaa tacgctcaac
     3061 ttcgaccgtt tcggaggcga tttggcgggc ggaccattca ctctcgcggg caaaatcacc
     3121 ctgccgaaac tgactgagcc gatgctggat ctctctctga aagcgaacag cgtgctggtg
     3181 gcccggaacg acaatctgac cgcacgcgtc gatgccgatc tcaaggttca agggccgctg
     3241 caaagcgcga ccgtcagcgg caatgttctc acgaccaaca gccggtttct gaaaaacatc
     3301 gacatcatcc cgatcggcct gccggggcgc cccgcgccgc aaccaccggc ggccaccccg
     3361 attctttcca ttccgacgcc gccgctgcgc gattggaagt tcgacctcgt ggtcaaaagc
     3421 aaggacccgt tcctgatcca gggaaacctg gcgaccggcg aagcgatcat cgatctcaag
     3481 ctgagtggga ctggcctgcg tcccgggctc caaggcgagg tgcggctcgc gaaattcgaa
     3541 gcgacgctgc ccttcagcac cctgacgatc gaatacggct ttctctactt caatcccgac
     3601 gatccactca atccgcggat cgagctgcac gggacctcgc tcctgcgcga ctacacggtt
     3661 cacgttttcg tttacggcac ggcgctcgcg ccggaagcgg tcttcaatag cgagccaccg
     3721 ctcccgcagg aggaaatcat ttcgctgctc gcgaccggag tgacccgcga ggaattgacc
     3781 ggcagcggca acgtcctcgt cggccgggcg ggcctgctcc tgatgaagca gctctaccgc
     3841 agggttttta agaacggcga cgagtcgacg acgaaaagcg actcgctctt taaccgcctc
     3901 gacgtcgagt taggtgcgat tgatccccgc accggccagc aaaccgcgac cgcgaagttc
     3961 aaggtgaacg atcacgtcgt tctcgtggga gacattgagg tgggcgggga ttttcgcggc
     4021 ctggtgaaat atctcatccg tttccgttag aagatgaagg gacgaagcgg atctgacgaa
     4081 acaggacatg cggaagcatg tctctccggg gggacgagct tccgcttgtc cagtttgaaa
     4141 tggcccgcca gtttccttct tttctcttgc ctaacgactg atttcgcgct cgcccaggac
     4201 gcggttcagc tcccggtgca gaagcaagaa gcggaggaaa aacgcgccgt tgcccaggcg
     4261 caggaaaagg tggcgcagca cgccaacctc gagattcgcg gcaacaccgc cttcgacgac
     4321 aaggtcctgc gctcccagtt gaaggagcaa atcgcaacca tcgaaaagta cggcctaacg
     4381 agcgcgcggg gcgacgacgc ggcttttttc ctcgagcttt tttacaaaaa acatggctac
     4441 gccgaggttc atgtgcgtta caccatcgag gacgcgagcc gcctccagct cgacatcgcc
     4501 gaaggcccgc tcgtccatct cgggaaaatc gttttcgtcg gccgccagca gttgccggaa
     4561 gagagacttc ttaattacgc ccttggcccg acgcgggagc gttactccaa ggcgcagtcg
     4621 ctcctccctt tcgtcagcgc ggatctcgag gaaggcgcca gtctggtgca gcgcttctat
     4681 gtctccgaag gcttcgtcga gtgcacgatc gagaaaccgc aagacgaata cgtgcggccc
     4741 gatctggtcg atgtccgcat cgtcgtgcac gagggccacc agtatttctt cggcaacatc
     4801 aattttgtcg gacccaccat ctatggcggg gaagcgttgc gcgggcagat gctcgatctg
     4861 ctgcagcggc cttataccga ggcacgactc gaggatatcc cgcgccgttt gcaggcctac
     4921 tacaaaacgc gcggatacta cgcggtcaaa gcggacgcgt ttggcgaccc gtcgctggcc
     4981 gtcgatggga aggtgcccgt gcgggtgacg attgagcccg gcgcgatcta ttacttcgac
     5041 ggcgtcacgg tgaaaggcac gcagaagctg cggccgagtt atctcgcaag acgattccgc
     5101 aaattccacg gcagaccgta cagccccgaa gcgattgaca agaggttccg tgagctgatg
     5161 cggacgagcc tcttcaacat tctccagatc aatccgacgc cggtcgacgg caaccatctg
     5221 cgactggata tcaatgtgga ggaagcgaag acgagagagt tcggattttc catcggatat
     5281 ggatcatttt ccggcgcgat cgtcggcgcg tcctacgcga atcgtgatct gtttggctac
     5341 gggcggccgg tcacgacctc ggttgagtat tcggagcgcg gatacaaagg cgaggtgacc
     5401 tacgaaaatc cttacctctt cgatacggac ttcgcgttca aggcgcgact ctcggcactg
     5461 acctacgact acgacggcta ttccaagttt gagcttggca gccgtttttc tctgagtcgc
     5521 aagatcaccg accaatatga agtcgggctg gtttacggtg cacggcatgt tgaagtcacg
     5581 gatgcgacga ttgacacggc actgctcggc cggacctcct acttcatcag cacgatcggg
     5641 tttacccaga cgcttgatct gcgcaaaaac ccgttcgtcg cgccgcgcgg cctgatcttt
     5701 gacaacacct tcgatctagc ttcgagcgcg attgggagcc agatcgaact catccgtggc
     5761 accgcgcggc tgagctacta tatcccgttc gcgtccgggc cgccggccct ggtgggagaa
     5821 gacctgacga agtcggcctt tcagcgatgg tttgagcgca gcctgctcgc cttcggcgcg
     5881 cgcagcggca tcgtttaccc gctcgataac gccgggaaaa gcaccgcgct ggccatcccg
     5941 atcgacgaac gatttttcag cggcggcggc acaacggtgc gcagttttac ggagcgcgac
     6001 ctcggaccgc acgatgcggg tggcgacccg attggcggcg aattctttac cattttcaat
     6061 gccgaatata ctttcccgat ctacggcgaa atcctcggtg cggtcttcgt cgatgccggc
     6121 aacctgctgc ccgatgcgcg ctcgccgggg ctgaacgaca tgcgctacgg agtgggcctg
     6181 gggctgcgct acaatctgcc catcggaccg gtccgcgtcg actacggctt caatcccgac
     6241 ccgcatgcta acgaggcctc gggagcgttc aatctcagtt tcggtttcgc attctagggg
     6301 gaagcctggg cgccgcgcgc gccgcacttc ctgcttgcct ggtgcctaac gaaaaacttg
     6361 cagatctgcg ttacttcgcg gggcgctgca gctctgccag cttctcttct gcgcgattga
     6421 cgtcggattt gcggccgctg ccaagggcaa ggagcttttt ctgccgctcc aggttgcgca
     6481 aggccgcctc gagctcggcg cggtgacgcc gctccgtcgc gatccgcgct gcttccgcga
     6541 tggcggcgat ctcgctttcg ttcccgggtt tgccggtttc cgccgcgact tttcgcgctt
     6601 cttccagccg cgcctcggcc agcatcgcct cgagttgcac aactctcagt acacgctgct
     6661 ccgcttccac tttggcgatg atgccggcgc ggaaaaggcg atccgcgctg gccgcccgct
     6721 ggcgcgcccg cgtcaggtca ttctcgatcg ccgacgtact cctcggatca agagttgatt
     6781 catccgacgc gaggttttgc aacaggagag gcggctcgat atccgcagga aggctatcgc
     6841 tttgtccggg gagctccggg tcctcgaggg cgaaggcggg cccggccgcg acgagcatgc
     6901 cgagcaccat gagccaccgc ctcatggcgg acaatatcat agcccgcgtg ggcgtcggcg
     6961 aaaaatctta ccaggcgggt gacccatcgc gaccttgccc atgcggctcg gacgatttat
     7021 gattgtagct gggccacaaa cgtccgtcgt ctgccaccta tcgattcgga aacaaacttc
     7081 ttttatggaa acagccatcg cagcacccca aaaaaccgcc gccgctcagc ccgattttct
     7141 cccgctcgag ggaaccgatt acgtggaatt ttatgtcggc aacgccaagc aggccgcgca
     7201 tttctacaaa accgccttcg gtttccagag tctggcttat gccgggcctg aaaccggcca
     7261 gaaagaccgc gtcagctatg cgatccggca acacaaattg acctttgtcc tgaccacgcc
     7321 gctgcggccc ggcacgccga tcgcggacca catctccaag cacggcgacg gcgtgaagta
     7381 tctggccttg cgcgtgcccg acgccacctc tgcctggaac gagacgacca agcgcggcgg
     7441 caaaagttac atgcagccga cgaggtcaaa ggacgctgat ggcgaagtcg tcatgagcgg
     7501 cattcacacc tatggcgaca ccgtgcacct gttcatcgag cgcaagaact accgtggtgt
     7561 ctttatgccc ggcttccgca ggtgggagtc gggctacaac ccgccggata ctggtctcca
     7621 gtatgtcgac cactgcgtcg gcaacgtcgg ctggaaccag atgaatccgt gggtcaagtt
     7681 ctacgaggat gtgatgggtt tccgcaatat cctgagcttc gatgacaagg atatctcgac
     7741 cgaatactcc gccctcatga gcaaggtcat gagcaatggc aacggcttcg tcaagttccc
     7801 aatcaatgaa ccggcggagg gaaagaagaa atcacaagtc gaggagtatc tcgatttcta
     7861 cgacggcgaa ggggtgcagc attgtgcgct cgcgaccaag gacatcgtga aaacagtgac
     7921 cgatctgcaa cggcgcgggg tcgagttcct gaacattccg accacctatt acgacacgct
     7981 cccggagcgg gtcggacaca tcgatgaaga tctcgagccg ctcaagcgcc tcggaatcct
     8041 ggtcgatcgt gataacgaag gttatctgct gcaaatcttc accaaaccgg tggaagaccg
     8101 gccgacgctc tttttcgaaa tcatccagcg caaaggcgcg aagagcttcg gcaagggtaa
     8161 cttcaaggcg cttttcgaag ccttggaaaa ggaacaggaa ctacgcggta acctttaaaa
     8221 gggagagatc aagcatgccg tattatcaaa gactcggcca gatgccgaag aagcatcaca
     8281 tctggtttca tcgcaacggt gcagcgccca cttacaagaa cgaaggcatc gcctacgaac
     8341 acgtcttcac cacggaaggt tttgatgaag cgtattcgat tgtctatcac ctgcgcccgc
     8401 ccacgcgcgt gcgcaacgtc gagctgatca agcattgggc gccgaagaag gtcacggaca
     8461 gcccgctgcg tcatcatcat ctcaagacgg cggacattcc gcgccggggc gatctttaca
     8521 ccagccgcat tccgatgtta ttcaaccagg acatcatcgc ctaccgggcg cggccggcga
     8581 agacctacga caagttcgag tactacaaaa acggtggcgc ggacgaagtc atcttcgttt
     8641 tcaagggtgg cggcacgctc gagacggttt tcggcaagct gccttatcgc gcggaagatt
     8701 acatcgtcat cccgcgcggg atcacttacc ggctcgtacc cgataacgtg caggaagaag
     8761 actatctcat cctcgaatcg gccggtccgg tgcgaattcc gaaacggtat ctcaatcacg
     8821 aaggccagat caaaatgggc gcgccctatt ccgagcgcga tctgcatgga ccgaaggaga
     8881 tcatcacgat cgacaaggag gaagatgtgg atgttctggt caaagacggc tcgcgtttca
     8941 cgcgcgtgac cctggcccag catcccttcg atgccgtcgg ttgggatggt tatctctatc
     9001 ccttcacctt caacgcgcag gacttcgagc cgatcacggg cacagtccac cagccgccgc
     9061 cgatccatca gacctttgaa gtccgcggct acgtcatttg cacgttcgcg ccgcgccttc
     9121 tcgatacgca tcccgaagcg gtcaagatcc cgtgggtgca tgataatgtg gaagccgatg
     9181 aggtgctgtt ttatgtccgc ggcaatttcg gatcgcgccg cgggatcgag agcggatcga
     9241 tcactctcca tccgcgcgga attccacacg ggccgcatcc gggcacgatc atggcgagca
     9301 aggatgcgct gcggaccgag gagctggccg tgatgttcga caccgcgcac accctcgaac
     9361 tgaccgagga atccctcgcg ctcgacgatc cgaagtatcc gctcagctgg ctcgactaag
     9421 ttggagcgcc cagggccaaa ctgcaggcgg gacgcctgct cgacctgaca gccgggacgg
     9481 ctgtgttacg ttctcgccgt gcaggagacc gacttcgaaa tctcggaacg cctaacgtcg
     9541 gcgcccgacc ggatgggaac cgatcgcctc gaggaaatgg agcgcctctt cggcgatctg
     9601 cttgacgcga tcggcgaaga ccggacgcgc gaaggtctgc gcaagactcc ggaccgcgcc
     9661 gcgcgcgcct tcgaatttat caccgccggc taccgccagt cggtcgagga agcggtcaac
     9721 ggcgcgatct tcgattccga cgcgagcgag atcgtgatgg tgaaagacat cgagctttac
     9781 tcgat
//
LOCUS       DATMNS010000058         4304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4727749, whole genome shotgun sequence.
ACCESSION   DATMNS010000058 DATMNS010000000
VERSION     DATMNS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4727749"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..919
                     /locus_tag="VNW28_02265"
     CDS             <1..919
                     /locus_tag="VNW28_02265"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HXA08775.1"
                     /translation="GDASWWDLSALDFHYWTQPLPTVLGWWADKSPEWFKHFSTAFVL
                     VVEIVGAFLVWLPRRLRQLGCGAIIFLQIVIGLTGNYAFFNLLTIALCLLLIDDVVWP
                     GGRRLTNRASAGRGWPLWVPAVMIVLTMPLNALLIFSGFGPGTRWPGPIGAMYGAIAP
                     FYVVNGYGLFRVMTKERPEIILEGSDDGLEWKPYEFKWKPGALNRPPGFVEPHQPRLD
                     WQMWFAALGDARQNPWFLGLVFRLLENSPDVVHLLGRNPFPEKAPRYLRAEIYRYRFS
                     TLAERRATGAWWERGEPRIYLPPVSLRGE"
     gene            978..1196
                     /gene="infA"
                     /locus_tag="VNW28_02270"
     CDS             978..1196
                     /gene="infA"
                     /locus_tag="VNW28_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXA08776.1"
                     /translation="MPKEEQIVTEGTVTQVLPGTMFRVDLAGQRNVLAHISGKMRKHF
                     IRIVPGDKVEVELSPYDLTKARIIFRAK"
     gene            1338..1628
                     /locus_tag="VNW28_02275"
     CDS             1338..1628
                     /locus_tag="VNW28_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08777.1"
                     /translation="MANEKKTTEDAAVPPQAQEKLQSGTSHARKAAEDFRSAAGAVAG
                     EYRGKAEEAWNEASDRVRSFQEDTEQYVRENPTKAVFSALGIGFVLGLIFRR"
     gene            1639..2064
                     /locus_tag="VNW28_02280"
     CDS             1639..2064
                     /locus_tag="VNW28_02280"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HXA08778.1"
                     /translation="MAGRPMPFRNPAGHAGLRQNLTSLVASLAQFLESRLQLAARESK
                     AALLHLLTLVACLIAAGALALFGYVFLVVFAIVEISHLLGNSWVWTALAVALLHFIAA
                     FVCLLVAWRQVKRPVFRETASVLKEDTEWLKNLDQTKAP"
     gene            2067..2423
                     /locus_tag="VNW28_02285"
     CDS             2067..2423
                     /locus_tag="VNW28_02285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08779.1"
                     /translation="MRQKIARSRELVVRDVGGLRYELDFARKFKQAFQRNTVVWVGAA
                     LAVGLLVALLRARPRKIYVNAAGRKVRSPGKSLLESGALLGILKLGVSLVQPMVLSYL
                     AKKVKSGGQSGAARRR"
     gene            2481..3584
                     /locus_tag="VNW28_02290"
     CDS             2481..3584
                     /locus_tag="VNW28_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXA08780.1"
                     /translation="MSASTSSSPGPAAVAPHVDQYLSIGQQGGASDIHLGVNAQPLWR
                     LSGNLQPIWPDGPRLTPQETAALATGFLNEAHRAQLAERGDVDFAYANDFGRFRTSVV
                     RQRLGIDLVFRIISPNVRSMDELGLPESLKLLTRFQNGLILVTGSVGSGKSTTLAALV
                     EQVNIERREHIITLEDPIEYVFQPKGCHITQREVHTHTKSFGAALRGSLREDPDVIMV
                     GEMRDLETVSLAITASETGHLVMGTLHTSNASRTLDRLLDVFPVDQQDQVRVMVSESL
                     RGVISQQLVPRIDGKGRVLALEILTNTPAVANVIREAKTFMLPGIIQTGRKQGMRLMD
                     DSLAQLFDQGLISAQEACSRAEQKPAMRQHVKG"
     gene            3641..4051
                     /locus_tag="VNW28_02295"
     CDS             3641..4051
                     /locus_tag="VNW28_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXA08781.1"
                     /translation="MGRMIHEELSRSIIGIAMEALNELKPGLDEKLYERAMIIELQRC
                     GRRVTVQPSFPVHYRSELIGNLVPDMIVDDLVIVDPKVVSTFTETHIAMLGYLNVTGL
                     QLALLLNFKNASLSWKRVINQTADKGTDPPNLCS"
     gene            4093..>4304
                     /locus_tag="VNW28_02300"
     CDS             4093..>4304
                     /locus_tag="VNW28_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HXA08782.1"
                     /translation="MSYIDKFFEILVNEGASDLHIGEGQPPKIRKHGDVMPIRDELVT
                     RDEAVAMLSEICGPKSWAIFEERGDF"
BASE COUNT          826 a         1311 c         1303 g          864 t
ORIGIN      
        1 cggcgacgct tcctggtggg acctgagcgc gctcgatttt cattactgga cgcaaccgct
       61 gccgacggtg ctcggctggt gggcggacaa gtcgcccgag tggttcaagc atttctcgac
      121 cgcctttgtc ctcgtagtgg aaatagtagg cgcgtttctc gtctggctgc cgcgccggct
      181 gcggcagctc ggttgcggcg cgatcatctt tttgcagatc gtcatcggcc tgaccggtaa
      241 ttacgccttt ttcaatcttc tcacgatcgc gctctgcctg ctcctgatcg acgacgtcgt
      301 ctggccaggc gggcgtcgcc taacgaatcg cgcgtcggcc ggccgcggtt ggccgctctg
      361 ggtgccggcc gtcatgatcg tcctgacgat gccgctcaat gcgctgctca tcttttccgg
      421 gttcgggccc gggacgagat ggccgggccc gatcggggcg atgtatggcg cgatcgcccc
      481 cttctacgtc gtcaacggtt acgggctctt tcgcgtcatg acgaaggagc ggccggagat
      541 catcctcgag gggagcgacg acggcctcga atggaaaccc tacgaattca aatggaaacc
      601 gggcgcgctc aatcggccgc cgggtttcgt cgagccgcac cagccgcggc tcgattggca
      661 gatgtggttc gcggccctgg gcgacgcgcg gcaaaacccc tggtttctcg ggctcgtctt
      721 tcgcctgctc gaaaattcgc ctgacgtcgt ccacttgtta ggcaggaatc ccttccccga
      781 gaaggcgccc cgttacctgc gggccgaaat ctatcgctac cgcttcagca ccctggcgga
      841 acgtcgggcg accggcgcct ggtgggagcg cggagagccg cgcatttatc tgccgccggt
      901 ctccttgcgc ggcgaataag cgcagccggc agggcgggcg tcacttgagt aagttccgtt
      961 aggcgctata ctcccgcgtg ccgaaagagg agcagatcgt gacggaggga acggtaacgc
     1021 aggtgttgcc ggggaccatg ttccgcgtcg atcttgccgg gcagcgcaac gtcctcgccc
     1081 atatctccgg caagatgaga aaacatttca ttcgcatcgt gcccggcgac aaagtggagg
     1141 tggaattgtc gccttacgat ttgaccaagg cgcggattat tttccgtgcg aagtaaccct
     1201 cggacgggtc gggaggggcc ctcgcgaaaa tcgtaagcgc aatccggcag cgaattccgt
     1261 atcccctcgg agagaagttt tattgtcgca agacagcaac cacccggcga atgaccgggt
     1321 tcaaccagga gaaaaatatg gctaacgaaa agaaaacgac agaagacgcg gcggtcccgc
     1381 cgcaggctca ggagaaattg caaagcggca cttcgcatgc ccggaaggca gccgaagatt
     1441 ttcgctcggc cgccggcgcc gtcgccggcg agtatcgcgg caaagcggaa gaggcttgga
     1501 atgaagcgag cgaccgcgtc cgcagtttcc aggaggatac cgaacaatac gtgcgcgaaa
     1561 atcctaccaa ggcggtcttt tccgcgctgg gcatcgggtt cgtgctcggt ttgatcttcc
     1621 gtcgttaggg agcgctggat ggcgggccgc cccatgccgt tccgcaatcc cgccggtcat
     1681 gccgggttgc gccagaatct gacctcgctg gttgcgtccc tcgcgcaatt cctcgagagc
     1741 cggctgcaac tggcggcgcg ggaatcgaag gcggcgttgc tgcacctcct gacgctcgtg
     1801 gcctgcctga tcgccgcggg tgctctggcc ctcttcggct acgtgtttct ggtcgttttc
     1861 gcgatcgtcg agatctcgca tttgctcggg aactcctggg tctggacagc gttggcggtg
     1921 gcgctactgc attttatcgc ggcctttgtc tgcctcctcg tggcgtggag acaggtcaag
     1981 cgacccgtct ttcgcgagac cgcgagcgtt ttgaaagagg ataccgaatg gctgaaaaac
     2041 ctggatcaaa cgaaagcccc ctagagttgc ggcagaaaat tgcccgctcg cgcgaattgg
     2101 tggtgcgcga cgtgggtggc ctgcgttacg agctcgattt cgcgcgcaaa ttcaagcagg
     2161 cctttcagcg caacaccgtc gtctgggtcg gggcggcgct ggcggtcggc ttgctcgtcg
     2221 cccttctccg ggcgcggccg cgcaagattt acgtcaacgc cgccggccgg aaagttcgat
     2281 cgccaggcaa gagtttgctc gagtccggcg ctctcctcgg aatattgaaa ctgggcgtgt
     2341 cgctcgtgca accgatggtg ctgagttacc tcgcgaaaaa ggtgaaaagc ggcggtcaat
     2401 cgggcgcggc gcgacgcagg taaccggatt tcaatttacg tctcacgagc ggttgcctat
     2461 tttctgaggc gccgctccgc atgtctgcaa gcacctcttc ctctcccggg ccggcggccg
     2521 ttgcgccgca cgtcgatcaa tatctttcca tcggccagca gggcggcgcc tccgatatcc
     2581 atctcggagt caatgcgcag cccctttggc gtctctccgg caatctccag ccgatctggc
     2641 cggatgggcc gaggctcacc ccgcaagaga ccgcggcgct cgccaccgga ttcctcaacg
     2701 aagcgcatcg cgcgcagctg gccgagcgcg gcgatgtgga ttttgcctat gcgaacgatt
     2761 tcgggcgctt ccgcaccagc gtcgttaggc aacgcctcgg gatcgatctc gtcttccgca
     2821 tcatcagccc caacgttcgc tcgatggatg aacttggttt acccgaaagc ctcaagttgc
     2881 tcacccgctt ccagaacgga ctcatcctcg tcaccggctc cgtcgggagc ggcaaatcga
     2941 ctactttggc cgcgctcgtc gagcaggtaa acatcgagcg ccgcgagcac atcatcacgc
     3001 tcgaggaccc gatcgaatac gtcttccaac ccaaggggtg tcacatcacg cagcgcgagg
     3061 tgcataccca cacgaagtcg ttcggcgcgg ccctacgcgg ctccttgcgc gaagatcccg
     3121 atgtcatcat ggtgggcgag atgcgcgacc tcgagaccgt ctcgctcgcg atcaccgcct
     3181 cggagaccgg ccacctcgtg atgggaacgc tccacaccag caacgcctcc cgcacgctcg
     3241 accgattgct cgacgtcttc ccggtcgatc aacaggatca ggtccgcgtc atggtgagcg
     3301 aatcgttgcg cggtgtcatc agccagcagc tcgtgccgcg gatcgacggc aaaggccgcg
     3361 ttctcgcgct tgagatcctc accaacacgc cggcggtggc gaacgtcatc cgcgaggcca
     3421 aaaccttcat gctgcccggc atcatccaga ccgggcgcaa acaaggcatg cgtttgatgg
     3481 acgattcgtt agcccaactc ttcgaccagg gcctgatcag cgcgcaggag gcctgcagcc
     3541 gcgccgaaca gaaaccagcg atgcgccagc atgtgaaagg atgattaccg cggattacgc
     3601 ggtgaattat ttttaaccac ggattacgcg gatttcacgg atggggagaa tgattcatga
     3661 ggagttgagc cgctccatca tcggcatcgc gatggaagcg ctcaacgaat tgaagccggg
     3721 attagacgag aaactgtatg aacgagcgat gatcatcgag ctgcagcggt gtggccgccg
     3781 tgtcacggtg caaccatcgt ttcctgttca ttatcgcagc gaactgattg gcaacttggt
     3841 ccccgatatg attgtcgatg atcttgtgat tgtcgatccg aaggttgtct cgacctttac
     3901 ggaaacgcac atcgcgatgc tcggctactt gaacgtcact ggcttgcaac tcgcactgct
     3961 cttaaacttc aagaacgcca gtctgagttg gaagcgcgta ataaatcaaa cagccgacaa
     4021 aggcaccgac ccgcctaacc tgtgttcctg actcttccca tccgtgaaat ccgcgtaatc
     4081 cgcggttaaa gaatgtccta catcgataaa tttttcgaaa tcctcgtcaa cgaaggcgct
     4141 tccgatttac acatcggcga gggccagccg ccgaaaatcc ggaaacacgg cgacgtcatg
     4201 ccgatccggg atgagctggt cacacgcgac gaggcggttg ccatgctgag cgagatttgc
     4261 gggccgaaaa gctgggccat ttttgaggag cgcggcgatt tcga
//
LOCUS       DATMNS010000059        15148 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_432696, whole genome shotgun sequence.
ACCESSION   DATMNS010000059 DATMNS010000000
VERSION     DATMNS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 15148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15148
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432696"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1681
                     /gene="glgP"
                     /locus_tag="VNW28_02305"
     CDS             <1..1681
                     /gene="glgP"
                     /locus_tag="VNW28_02305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HXA08783.1"
                     /translation="DKKLDFYSAVQIVASANIFTTHTPVPAGNDSFPRDMMRKYFGEF
                     AKDLGLPFDELLSFGQTRTDRSEPFSMTILALRLSRHSNGVSRLHGDVSRSLWRDVWN
                     GVPVHEVPITSITNGIHTKTWMAPEFSALYTKYLGNWEEHLTEPDFWRGVLEIPDAEL
                     WETHQKLKLRLVNFVRDRVRARRERIGESPESIRTVNSILDPEILTIGFARRFATYKR
                     GALLFSDPDRLLRMLNDLARPVQFVFAGKAHPRDEGGKALIQEVYKLTRESGFDGRIV
                     FVEDYDTYIARRLVQGVDLWLNNPLRPLEASGTSGMKLPPNGGLNLSVLDGWWCEGYN
                     DKNGWAIGAEIEDGDSEFQNEVDASSLYQLLENQIIPLYYAKPDGRLPLAWLQLMRES
                     IRSVTPTFNTHRMVKEYTERLYLRAARAHAEFSRDGWQAATELSQWKAQMRKDWPQVR
                     ISDVQIGNKDRQNIQVGDSLQVSAQVHLGALDPRHVRVEAYHGEAENDALRHPSVTVL
                     GEGKRLEGDGDYIYEGTVPAAESGTYGFSVRVVPTYPHLLQAHELRLIAWA"
     gene            complement(2248..3765)
                     /locus_tag="VNW28_02310"
     CDS             complement(2248..3765)
                     /locus_tag="VNW28_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HXA08784.1"
                     /translation="MRRVFGLETEYGITVVGAESIDVVAESIELVRGYTEHGAHMKWD
                     YDLEDPHRDARGFHAAELLQDTDESAYFEIDRKRPLSFEEIKSDLVLSNGARFYNDHA
                     HPEYSTPECTTLRELVAQDKAGERILAECARRRNLRLPNDQAVRLYKNNTDFSGHSYG
                     CHDNYLMRRDVPWDRIVAGVLPFLVTRQIFAGAGKMGIEAESAAGQPGIYQISQRADF
                     FATLVSIDTMNRRPLVNTRDEPHADSSRYRRFHVILGDANMSEWATALKIGTTALVLD
                     LIERGGAPQIEIAQPINATKSISRDQTYDWIIELTDGRKISAIDVQRIYLGAARKSTR
                     NPDAEVEWLLREWETVLNDLERDVRLCRDRVDWVAKKELLEALLREEELSWIDPWLQS
                     IDLEYHNIVPDEGLHYELVRQGAMRQFVSEEEIKHAIFAPPADTRAFFRGRSVARFNS
                     QIKSIQWDEIVFAAGAIPQRVPLPHPSDNPRLAALNKTVGEAADYREFIRTVATL"
     gene            complement(3855..4916)
                     /gene="hypE"
                     /locus_tag="VNW28_02315"
     CDS             complement(3855..4916)
                     /gene="hypE"
                     /locus_tag="VNW28_02315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HXA08785.1"
                     /translation="MDEATDSLLTASCPLPIADYREIVLAHGSGGKLSHQLISKIIVP
                     QFANELLAPMHDGAIFPLGGERVAFSTDSFVVSPIFFPGGDIGKLAVHGTVNDLAMCG
                     ARPLYLSVGFILEEGTPMEDFWRVVQSMRAAADEAGVQLVTGDTKVVDRGKADKIFIN
                     TAGIGLVPKGVTIHPARARAGDKVILSGSVAVHGIAIMSVREGLEFETEIASDTAALN
                     GLVQDLLAACGDVHVLRDPTRGGVTSALTEIAQAAGVGIILQESAIPISEEVKGACEI
                     LGLDPLYVANEGKLIAIVPPDDAEAALAAMRQHPLGKEAALIGEVVSDHAGFVVMKTR
                     VGGTRVVDMLSGEQLPRIC"
     gene            complement(5052..5678)
                     /locus_tag="VNW28_02320"
     CDS             complement(5052..5678)
                     /locus_tag="VNW28_02320"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXA08786.1"
                     /translation="MSASLETATERLTEPFREKVLRKCGESMATKEKFFSHYADSLDQ
                     MSRALAARFDKGGRLFVMGNGGSLCDALHIAVEFNHPIIEKRLAFPAIPLMTDIATMT
                     AISNDLDFTRVYVNQLRLHATPNDIAMVFSTSGKSPNLIYALETAREQQLLTVAFSGK
                     DGGRFPAVADYCYIVPSYSIHRIQEAHATAVHIIWDLVHIALGAEDVT"
     gene            complement(5681..6820)
                     /gene="hypD"
                     /locus_tag="VNW28_02325"
     CDS             complement(5681..6820)
                     /gene="hypD"
                     /locus_tag="VNW28_02325"
                     /inference="COORDINATES: protein motif:HMM:TIGR00075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HXA08787.1"
                     /translation="MKYLDEYRDEALAKTIVDEIKRTVTKPWVLMEVCGGQTHSIVKY
                     GIDRLLPSEVELVHGPGCPVCVTSLEMIDKALEIARRPDVIFCSFGDMLRVPGSRDDL
                     LILKSRGADVRVVYSPIDCLKIARANPEKKVVFFAIGFETTAPGNAMSVWQAREQGIK
                     NFSVLVSHVLVPPAIASILQSPLNRVQGFLGPGHVCTVMGYREYEPIAARYRVPIVIT
                     GFEPIDILEGTLMTLRQLEAGKAEVENQYPRVVSRDGNQVAQDLVNKVFEVGDRKWRG
                     VGSIPKSGYKLRYEFREHDAERIFDVQEIDTREPEICISGLVLRGVKKPHDCPAFGSL
                     CTPEHPLGATMVSAEGACAAYYAYGRHLQKPAATSKEAATLARAN"
     gene            complement(6817..7173)
                     /locus_tag="VNW28_02330"
     CDS             complement(6817..7173)
                     /locus_tag="VNW28_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HXA08788.1"
                     /translation="MCLAIPGKLIEISADPNGVRMGKANFGGIVKQVCLEYTPEVALG
                     DYVLVHVGFALSKVDEAEAARTYQALERMEQLQELDIPDVRDDGSAPTTPISTNGKHP
                     SDPADRAGEPIASRVP"
     gene            complement(7366..9651)
                     /gene="hypF"
                     /locus_tag="VNW28_02335"
     CDS             complement(7366..9651)
                     /gene="hypF"
                     /locus_tag="VNW28_02335"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HXA08789.1"
                     /translation="MKTELAATTHRVRLKMVLRGAVQGVGFRPFVYRLARNLGLDGWV
                     LNSAEGVFLEVEGKPEILAEFARRLRAEQPARALIQSCESSQLDALGYEGFEIRESTD
                     DGGKSALILPDIAPCADCLAEIFDPGDRRFRYPFTNCTNCGPRFSIIEALPYDRTNTA
                     MKKFPMCEDCACEYRDPADRRFHAEPIACPRCGPALQLWDSLGKSVARADAALRPATA
                     AIRSGQIVALKGVGGFQLLVDARSDEAVQRLRERKHREEKPFAVMFPSLEEVRGQCAI
                     SELEERLLTSPEAPIVLLARASSSLAGSLAPGNPNLGVMLPSSPLHHLLLRELNFPVV
                     ATSGNLSEEPICTEEDEARERLRGLADFFLVHNRPIVRHVDDSIVRVMHGREMMLRRA
                     RGYAPLPITLQRARPTVLAVGAHLKNTVALSLGRDVFVSQHIGDLATKQAHDAFTRSA
                     ADLPRLYEAKPEVIAHDLHPEYLSTKYARQQPGPKVAVQHHWAHIASCMAENEIIPPL
                     LGVAWDGTGYGLDRTIWGGEFFLVQENSCRRAAHFRTFRLPGGEAAVKEPRRSALGML
                     HEIFGDELWQRDDLLADFTPAERKTLRGMLRKQVNSPITSSAGRLFDGVAAFVGLRTR
                     STFEGQAAMELEFAVEDGIDESYGFAWHERAPLVIDWEPTLRSLLEDRRRRTPTGVIA
                     ARFHNTLAEMILAVARHLDTSKVALSGGCFQNRYLTERVIEQLLTAGIQPYWQQRVPP
                     NDGGIALGQVWLATSPLREFK"
     gene            complement(9648..10427)
                     /locus_tag="VNW28_02340"
     CDS             complement(9648..10427)
                     /locus_tag="VNW28_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08790.1"
                     /translation="MRTLFPILLFGFFLGMRHATDSDHVIAVTTIVSRQRHIGSAAWT
                     GIFWGIGHSLTLLVIGGAIILFGIVVPERLGMSLEFCVALMLILLGALNLNATFRAVE
                     SASAGRDHDGVHQHPHRHGDYVHSHAHGHEGATHGHREDAVPPARLDRWFGSSRFYQS
                     LRPLIIGTVHGLAGSAAVALLVLPIIHDPIWAMAYLVIFGLGVIAGMMLITAAIAVPV
                     SYSTRFQFLHRQLGTAAGVLSLGFGCFLVYQIGFVDRLFVR"
     gene            complement(10424..10819)
                     /locus_tag="VNW28_02345"
     CDS             complement(10424..10819)
                     /locus_tag="VNW28_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HXA08791.1"
                     /translation="MHEFSIASGLVEKLLDFAEKNPDKKIVEVRVAIGEFTQIEEEQL
                     RFCYESIITEMPIAGSRLTIEPVLGKVACPDCSYSGLPKYWDGALHGVPVATLECPGC
                     GKTARVIEGEECAIRSVRFMPNEIAGALS"
     gene            complement(10941..11633)
                     /gene="hypB"
                     /locus_tag="VNW28_02350"
     CDS             complement(10941..11633)
                     /gene="hypB"
                     /locus_tag="VNW28_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HXA08792.1"
                     /translation="MAQESAAQVMLEASPDEIYDIELEHSLLEANAKLAQENRALLDR
                     SGITAIDFMGAIGSGKTTLITKLVERLKDRMGVAVFNGDATTSNDADAISKQNVPVVQ
                     IATINGCHLDANLVGKGLRKIKLDNLKLIFIENIGNLICPAEFPLGSKARVVVVSVTE
                     GPWMVRKHPHMFLGAQLVVINKIDLAEAMEVSVEQLTNDVHVLKPDIKVIPTSCKNGT
                     GLDEVAATLLAV"
     gene            complement(11636..13378)
                     /locus_tag="VNW28_02355"
     CDS             complement(11636..13378)
                     /locus_tag="VNW28_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HXA08793.1"
                     /translation="MSWDPITRIVGSLGIYTKIDFDSKKVVECYSTSSIFRGYSIFMQ
                     NKDPRDAHFITSRICGICGDNHATCSVYTQNMAYGVKPPALAEWIVNLGEAAEYMFDH
                     NIFQDNLVGVDFCEMMVKETNPSVWAKAQTTLAPHSNDHGYRTIADIMTALNPFSGEF
                     YRETLHVSRYTREMFCLMEGRHVHPSTLYPGGVGTVPSIQIFTDYTVRLMRYIEFMKR
                     CLPLHDDLFDFFYEALPGYEEVGRRRILTACWGSFQDPDVCDYDYKTMEKWGNAMFVT
                     PGMVIDGELVTTNLVDINLGIRILLGSSYYDDWDNSETFVKNDPLGNPVDKKHPWNQT
                     TIPKPQRRDFGGNYTWVMSPRWLDKRTGDHLAMDTGGGPIARLWATALAKLVNIGYVE
                     ATGHSVKMRLPKTALKGEVEFEWKVPKWSNAIERDRARTYFQVYAACCALYFLEKAMA
                     EVHAGRTATFTPFKVPDEAIGCGFHEAVRGVLSHHMVIRKGKIANYHPYPPTPWNANP
                     RDIYGTPGPYEDAVQNTPIFEENGPDNFKGIDIMRAVRSFDPCLPCGVHMYLGGGKVL
                     KQMHTPTALAGLAR"
     gene            complement(13482..14546)
                     /locus_tag="VNW28_02360"
     CDS             complement(13482..14546)
                     /locus_tag="VNW28_02360"
                     /inference="COORDINATES: protein motif:HMM:NF013242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08794.1"
                     /translation="MVAANTPFKHRESAVKEIHVIWMTTGLGCDGDSVSITAATQPSI
                     EDLVMGNIPGLPKVHVHNPVIAYEVGDEFMKHWFDAAAGKLDPFVLVMEGSIPNEKIK
                     KEGYWAALGTDPDTGQPITTNEWIDRLAPKALGIVACGTCATYGGIHAMEGNPTGAMG
                     LADYLGWGWRSKAGLPIVNVPGCPVQPDNMMETLLYLLYQVAGLAPLIPLDEQLRPTW
                     LFGKTVHEGCDRAGYYEQGDFAHEYGSPKCLVKIGCWGPVVNCNVTKRGWMAGIGGCP
                     NVGGICIGCTMPGFPDKFMPFMDAPPGASLSTKVVGLWGGALRKLRAITNTTVNKEPK
                     WRHPRAELTTGYEPRNYARN"
     gene            complement(14801..>15148)
                     /locus_tag="VNW28_02365"
     CDS             complement(14801..>15148)
                     /locus_tag="VNW28_02365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HXA08795.1"
                     /translation="VDASPQGSAPGTIYLIEPDQKKLDELPDEAVNAHSMNPVRVLQL
                     VRSLGGAPGWLRVVGCEPAILEAEEGAMGLSEKVQAAVQPAIELIESLIGNILKGPEA
                     RSPQSIRECETIP"
BASE COUNT         2973 a         4541 c         4541 g         3093 t
ORIGIN      
        1 cgacaagaag ctcgattttt attcggcggt gcaaatcgtc gcgtcggcca acattttcac
       61 aactcacacc cccgtccccg ccggcaacga ttctttcccg cgcgacatga tgcggaaata
      121 tttcggcgag ttcgccaaag atcttggcct tccgttcgat gagctgcttt cttttggtca
      181 gacgcgaacc gatcggtccg aaccgtttag tatgacaatt ctcgcgctgc gattgagccg
      241 tcattcaaac ggtgtgagta gattgcacgg cgacgtcagc cggtccctct ggcgcgatgt
      301 ttggaatggc gtgccggtcc acgaagtgcc gatcaccagc atcaccaacg gcattcacac
      361 caagacctgg atggcgccgg aattttccgc tctctacacg aaatacctgg gcaattggga
      421 agagcatttg accgagccgg atttctggcg cggcgtgctc gagattcccg atgccgagct
      481 gtgggagact caccagaaac taaaactgcg cctcgtgaac ttcgtgcgcg accgcgtgcg
      541 cgcgcggcgg gagcggattg gcgaatcgcc cgagtcgatt cgcacggtga actccattct
      601 cgaccccgag atcctcacca ttggtttcgc gcggcgcttt gccacctaca agcgcggcgc
      661 gctgctcttc agtgacccgg atcggctgct ccgcatgctg aacgaccttg cgcgaccggt
      721 ccagtttgtc tttgcgggca aagcgcatcc ccgggacgag ggcgggaaag cgctcattca
      781 ggaagtttac aaattgaccc gggagtcggg attcgatggc cgcatcgtct ttgtcgagga
      841 ctatgacact tacattgcgc gtcgtttagt ccagggggtg gatctttggc tgaacaaccc
      901 actccgccca ctcgaggcca gcggcaccag cgggatgaag cttccgccca acggcggtct
      961 taatctcagt gtgctcgatg gctggtggtg tgaaggctat aacgacaaaa atggctgggc
     1021 gatcggggcc gagatcgaag atggcgacag tgaatttcag aacgaagtgg acgcgtccag
     1081 tctttaccaa ctcctcgaga accagatcat cccgctctat tacgccaagc cggacggccg
     1141 ccttcccctc gcctggttgc agttgatgcg cgaatcgatt cgcagcgtca ctcccacctt
     1201 taacactcat cggatggtga aggagtacac tgagcggctg tacctccgcg ccgccagggc
     1261 tcacgccgag ttttcgcgcg acggctggca ggcggcgact gagctgagtc agtggaaggc
     1321 gcagatgaga aaagactggc cgcaggtgcg catcagcgac gtccagattg gcaataagga
     1381 tcggcaaaat atccaggtgg gcgattcgct tcaggtctcg gcccaggttc atctcggcgc
     1441 gctcgacccc cggcatgtgc gggtcgaggc ttatcacggc gaagcggaaa atgacgcgtt
     1501 gcgccatcct agcgtgacgg tgctggggga aggaaaacgc ctggaggggg atggagatta
     1561 catttacgaa gggaccgtcc cggcggcaga gagcggcacc tatggcttca gcgtgcgcgt
     1621 tgttccgacc tatccgcatt tgctccaggc ccatgaactc cggctcattg cctgggcgta
     1681 gggcgcacct tcgtcctttg ctccgttagt taacttttgc tccgtcgctt caccggtccg
     1741 tctgatggca cgcggaggcc gcatgaaggc ttacaaattc ttttgcagtt agagcaaaac
     1801 attgcttgct caatcttcag tatggcttat aactgcgcca tggatctatc tctcaaacaa
     1861 ctggaagaag cagtggccgt tagacgacaa atcgaccaac tgcgtagtcg attacatagt
     1921 ctgctaggcg gcgccagcgc accgagcagt agtggcagtt caaccgcgtc gagcgcgccc
     1981 cgcgctccgc gcaagcgccg cggcggcctt tccgccgccg gccgtgaacg gatcgctgcc
     2041 gccgcccgcg cacgctgggc tcgggccaaa gccgctgccg gtcctgcgtc aagtggcaag
     2101 aagtctggcg gcaagaagaa aaaaggtggg atcagcgcgg cgggccgtcg caagctctcg
     2161 caaatgatga aggcccgctg ggcggcccgg cgcagagctc agggctaatt tgttaggcaa
     2221 gtttccgcgc gcgctggctg acgccaccta gagcgtcgcc acagtgcgga taaactctcg
     2281 ataatccgcg gcctcgccga ctgttttgtt cagtgcggcg agccgcggat tgtcactggg
     2341 atgcggcagg ggcacgcgct gtggaatcgc cccggcggca aagacgattt cgtcccattg
     2401 gatcgatttg atctgcgagt taaaacgggc caccgaccga ccgcgaaaga aagcccgcgt
     2461 atcggccggg ggagcgaaga tggcgtgctt gatttcttct tcgctgacga actgccgcat
     2521 cgctccttgc cggaccaact cataatggag gccctcgtct ggaacgatgt tatgatattc
     2581 gagatcgatc gattgcagcc agggatcgat ccaggataac tcttcttccc tgagcaacgc
     2641 ctccagcaac tctttcttcg ctacccagtc gacacggtcc cggcagagcc ggacatccct
     2701 ttcgagatcg tttaacacgg tttcccattc ccgaagcagc cattccacct ccgcatcggg
     2761 attccgcgtc gacttcctgg ctgcgcccag atagatgcgc tgcacatcga ttgctgagat
     2821 cttgcgtcca tccgtcaatt cgatgatcca gtcgtaagtc tggtcgcggc tgatcgactt
     2881 ggtcgcgttg atcggctgcg caatctcgat ctgaggcgcg ccgccgcgtt cgatcaggtc
     2941 gagcaccagt gcggtggtgc cgattttcaa cgccgtcgcc cactcactca tgttcgcgtc
     3001 gccaaggatg acgtggaaac gccggtagcg gctggagtcc gcgtgcggct catcgcgggt
     3061 gttgaccagc ggtcgccggt tcatcgtatc aatgctcacc agggtggcga aaaaatccgc
     3121 gcgctgcgaa atctggtaaa tcccgggctg ccccgcggca ctctccgctt cgattcccat
     3181 cttgcccgcc ccggcgaaaa tttgccgggt cacgaggaag gggagcacgc cggcgacgat
     3241 ccgatcccac ggcacgtccc gccgcatcag gtaattatcg tggcaaccat agctatgtcc
     3301 tgagaaatcc gtgttgttct tgtaaaggcg caccgcctgg tcgttaggca atctcaggtt
     3361 gcggcggcgg gcgcactccg ccaggatgcg ctcgccggcc ttatcctgcg ccaccagttc
     3421 gcgcagggtg gtgcattccg gggtcgagta ttccggatgg gcgtgatcgt tgtagaagcg
     3481 cgcgccattg ctcagcacca ggtcgctttt gatttcctca aaactaagcg gtcgcttgcg
     3541 atcgatctcg aaataggccg actcatccgt gtcctgcaga agttccgcgg catggaaacc
     3601 acgcgcatcg cgatgcgggt cttccagatc gtagtcccat ttcatgtgcg cgccgtgttc
     3661 cgtgtaaccg cgcaccagct cgatcgattc cgccaccaca tcgatcgact ccgctccgac
     3721 caccgtgatc ccatattccg tttccaatcc gaagacgcgt ctcatccttc gccaatatat
     3781 acgcgagtgc accgcggcgc gccgatggga accgactcgc agggcggttg aggaagcctt
     3841 ctggcagcgg ctccctaaca aatgcggggc aactgctcgc ccgagagcat gtccaccacc
     3901 cgggtgccgc ccacgcgcgt cttcatgacc acgaaaccgg cgtgatcgct caccacctcc
     3961 ccgatgaggg cggcttcttt gcctaacgga tgctgacgca tggcggccag cgccgcttcc
     4021 gcgtcgtccg gcgggacgat cgcgatcagt tttccttcgt tcgcgacata caaaggatcg
     4081 agcccgagga tctcgcacgc gcctttgact tcctcgctaa tcggaatggc agattcctgc
     4141 aggatgatac cgacgccggc tgcctgcgcg atctccgtca aagcgctggt cacgccgccg
     4201 cgcgtcggat cgcgcaggac gtgaacatcg ccgcaggccg ccaggaggtc ctgcaccagt
     4261 ccgttgagcg cggccgtgtc actcgcgatc tcggtttcaa attccaatcc ttcacggacc
     4321 gacataatcg cgatcccgtg caccgccacc gacccgctga ggatgacttt gtcgcccgcc
     4381 cgagcccgcg cgggatggat ggtgacgccc ttgggcacga ggccaatccc ggccgtgttg
     4441 atgaagattt tgtcggcctt gccgcggtcc acgaccttcg tatcgccagt caccaactgc
     4501 acgcccgcct catccgcggc cgcgcgcatg gattgcacga cgcgccagaa atcctccatc
     4561 ggcgtccctt cctcgaggat gaacccgacc gacaagtaga gcgggcgcgc gccgcacatc
     4621 gcgagatcgt tgaccgtccc gtgcacggcc agttttccaa tgtcgccgcc cgggaaaaaa
     4681 atcggactaa cgacgaagga atccgtgctg aaggcgaccc gttcgccgcc taacgggaaa
     4741 atggcgccgt catgcatcgg cgcgagcagt tcgttggcaa actgcggcac gatgatcttc
     4801 gaaattagct ggtgactcag cttccctccg ctcccgtggg cgagcacgat ctcccggtaa
     4861 tcggcaatcg gaagcgggca ggaggcggtg agaagtgagt cggtggcttc gtccacgctt
     4921 ttactctaat gacgaattcc gaatgacgaa gctcgaatca atgacgaacg accaagtttg
     4981 caggatcgcc cgcttcgtca ttcggaattc ggctttcctt cggatctcgt cattcggcct
     5041 acgtcattcc gtcaggtgac gtcttccgcg ccgagggcga tgtgaaccaa atcccagata
     5101 atgtgcacgg cggtggcgtg cgcttcctgg atgcggtgga tgctgtagga aggaacgatg
     5161 tagcagtaat cggcgaccgc gggaaaacgg ccgccgtcct tgcccgagaa ggcgacggtc
     5221 aggagctgct gctcgcgcgc tgtttccagc gcgtaaatga ggttgggcga cttgccgctc
     5281 gtgctgaaga ccatcgcgat gtcgttaggc gtggcgtgca gccggagctg attgacgtag
     5341 acgcgggtga aatcgaggtc gttgctgatc gccgtcatgg tcgcgatgtc ggtcatgagc
     5401 gggatggcgg ggaaagcgag tcgtttttcg atgatcggat gattgaactc gaccgcgatg
     5461 tgaagcgcgt cgcagagact gccgccgttg cccatgacga agagccggcc ccctttgtcg
     5521 aatcgtgctg ccagggcgcg cgacatctga tcgagtgagt cggcgtaatg agagaaaaat
     5581 ttctccttcg tcgccatgct ctcgccgcat ttgcgcagca ctttttcgcg aaacggctcc
     5641 gtcagccgct cggtagccgt ctcgagcgaa gcgctcatgc ctagttcgcc cgggcgaggg
     5701 tcgctgcctc tttcgaggtg gccgccggct tttgcagatg acggccgtag gcgtaatagg
     5761 cggcgcaggc gccctccgcc gagaccatcg tcgcgcctaa cggatgctcc ggcgtgcaga
     5821 gcgaaccaaa ggcgggacaa tcgtgtggtt tctttacccc gcgcaggacc aggccgctaa
     5881 tgcagatttc cggttccctg gtgtcgattt cttgcacgtc gaaaatgcgc tcggcgtcgt
     5941 gctcgcgaaa ttcgtagcgc agcttgtagc cgctttttgg aatcgaaccg acgccgcgcc
     6001 atttgcggtc gcccacttcg aaaaccttgt tgaccaggtc ctgtgcgacc tgatttccgt
     6061 cgcggctaac gacgcgcgga tattgatttt cgacctccgc cttgccggct tcgagctggc
     6121 gcagggtcat gagcgtgcct tcgagaatat cgatcggctc gaaaccggtg atcacaatcg
     6181 gtacccggta acgggccgcg attggctcgt actcccgata gcccatcacc gtgcagacat
     6241 gacctggtcc gaggaatccc tgcacgcgat tgagcggcga ttgcaggatg gaagcgatcg
     6301 ccggtgggac gagcacgtgc gagacgagta cggaaaagtt cttgatcccc tgctcgcgcg
     6361 cctgccagac cgacatcgca ttgccaggcg cggtggtctc gaaaccgatc gcgaagaaga
     6421 cgactttttt ctccggattg gcgcgcgcga tcttcagaca atcgatcggc gaatagacga
     6481 cgcggacgtc ggcgccgcgc gacttcaaaa tgaggagatc gtcccttgag ccgggcacgc
     6541 gcagcatgtc gccaaaggaa caaaaaatga cgtccggccg gcgcgcgatc tcgagcgcct
     6601 tgtcgatcat ttccagcgag gttacgcaaa ccggacagcc cgggccgtgg accagctcga
     6661 cctcggaagg gagtaaccgg tcgatgccgt acttcacgat cgagtgagtt tgtccgccgc
     6721 agacttccat caagacccat ggtttggtca cggtgcgctt gatttcatcg acgatcgttt
     6781 tggcgagcgc ttcgtcgcgg tattcgtcga gatacttcat gggacacgag aagcaattgg
     6841 ttcgcccgcg cgatcggctg ggtctgaagg atgtttgccg ttggtggaaa tcggggtcgt
     6901 cggggctgag ccatcgtccc gcacatcggg gatgtcgagc tcctggagtt gctccatgcg
     6961 ctcgagtgcc tggtaagtcc gggccgcttc cgcttcatcg accttgctga gcgcgaagcc
     7021 gacgtggacc aaaacgtaat cgccgagtgc gacctccgga gtgtattcga ggcaaacctg
     7081 tttcacgatc ccgccgaaat tggctttgcc catcctgacg ccgttcggat ccgcgctgat
     7141 ttcaatgagt ttaccgggaa tcgccagaca catgttccgt cttcctttcg ttgggggcgg
     7201 cgaccttgct gatcgccact ccgtcgcaga ctaatactct atcaccgcgc acggcgtccg
     7261 tcaggagtcc aagaggtatt ttttgagtcg ccccgtgcac tcgaatcctc ccgacgggca
     7321 ggccgttttc cgaggagact tcgatgagtt cgccataaag cagattcatt taaactcccg
     7381 gagagggctc gtggcgagcc aaacctgccc gagcgcgatc ccaccatcgt taggcgggac
     7441 gcgttgctgc cagtagggct gaatccctgc ggtcaagagc tgttcaatca cgcgctcggt
     7501 caaataacgg ttctgaaaac atccgccgct cagggcgact ttggacgtat caaggtgtcg
     7561 ggcaaccgcc agaatcatct cggcgagggt gttgtggaag cgggcggcga tcactccggt
     7621 tggcgtcctt cgacgccgat cctccaggag cgaacgcagg gttggttccc aatcgatcac
     7681 gagcggtgct ctctcgtgcc aggcaaagcc gtagctctca tcgatcccgt cctcgacggc
     7741 gaactcaagc tccattgccg cctgaccctc gaaagtcgaa cgagtgcgta atccgacgaa
     7801 cgccgccacc ccatcaaaga gtcggccggc gctcgaagtg atcggagaat taacctgctt
     7861 tcgcagcatg ccgcgcagcg tcttccgctc ggccggcgtg aaatccgcga gcaggtcgtc
     7921 ccgctgccac aattcgtcgc cgaaaatttc atggagcata ccgagcgcgc tgcgacgcgg
     7981 ttctttcacg gcggcttctc ctcccggcag gcgaaaagtg cggaaatggg cggcgcgccg
     8041 gcaggagttt tcctgcacga gaaagaattc gccgccccag atggtgcgat ccaggccgta
     8101 gccggttccg tcccaggcca cgccgaggag cggaggtatg atttcatttt ccgccatgca
     8161 ggaagcgatg tgcgcccaat gatgctgcac cgcgaccttt ggaccaggct gctgccgcgc
     8221 gtatttggtc gagagatatt ccgggtggag atcgtgggcg atcacctccg gtttcgcctc
     8281 gtagaggcgc ggcaggtccg cggccgaacg ggtaaacgcg tcgtgcgctt gtttggtcgc
     8341 gaggtcgcca atgtgctgac taacgaaaac gtcccgcccc aggctcagcg ccacggtgtt
     8401 tttcagatgt gccccgacag ccagcacggt cggtcgggcg cgctgcagcg tgatcgggag
     8461 cggcgcgtaa ccgcgggcgc ggcgcagcat catctcccgc ccgtgcatga cccgaacaat
     8521 ggaatcatcc acgtgcctaa cgatcgggcg gttgtgcacg agaaagaaat cggccaggcc
     8581 gcggaggcgc tctcgcgcct cgtcttcctc cgtgcagatc ggctcctcgc tcagattacc
     8641 gctcgtggcc acgaccggga aattcaattc gcgcaggaga agatgatgga gcggtgaaga
     8701 gggaagcatg acgccgagat tgggatttcc aggagcaaga gacccggcca gcgaagagct
     8761 ggcccgcgcc agcaagacga tcggcgcctc cggcgaagtg agcagacgct cttcgagttc
     8821 ggagattgcg cattgcccgc gcacctcttc gagcgacgga aacatgactg cgaacggttt
     8881 ttcttcgcga tgcttgcgct cgcgcaggcg ctgcacggcc tcgtcgctgc gcgcatcgac
     8941 gagcagttga aaaccgccga cgcccttcag cgcgacgatt tgaccagaac gaatcgctgc
     9001 cgtggccggg cggagggcgg catctgcccg ggccacgctt ttgcctaacg agtcccaaag
     9061 ttgcagcgct ggaccgcaac gcgggcacgc gattggttca gcgtggaagc ggcggtcggc
     9121 cggatcgcgg tattcgcagg cgcaatcctc gcacatcggg aatttcttca tcgcggtgtt
     9181 ggtgcgatcg tagggcagcg cttcgatgat ggaaaagcgc ggcccgcaat tggtgcagtt
     9241 cgtgaaggga tagcgaaagc ggcgatcgcc cgggtcgaaa atctcggcca gacaatccgc
     9301 gcagggcgcg atgtccggca aaatcagcgc gctcttcccg ccgtcatcgg tactctcgcg
     9361 aatttcgaaa ccctcgtagc cgagcgcgtc gagctgggac gactcgcaac tttgaatcag
     9421 ggcgcgcgcc ggttgctccg ctcggagacg gcgcgcgaat tcggccagga tctcgggctt
     9481 gccttcgact tcgagaaaga cgccttccgc ggaattgagc acccagccgt ccagccccag
     9541 attcctggcc agacgataaa cgaaaggccg aaaccccacg ccctggaccg cgccgcggag
     9601 caccattttc aggcggacgc gatgcgtcgt cgcggccagt tccgtcttca cctaacgaaa
     9661 agacggtcga cgaaaccaat ctggtaaacc aggaagcagc cgaaaccgag actgagcacg
     9721 ccggccgccg tgccgagctg gcggtggagg aactggaacc gggtcgaata ggagaccggc
     9781 accgcgatcg cggccgtgat cagcatcatc ccggcgatga ccccgaggcc gaagatcaca
     9841 agataagcca tggcccagat cgggtcgtga atgatcggca gaaccaggag cgcgaccgcg
     9901 gccgagccgg caagtccgtg cacggtgccg atgatcaagg ggcggagcga ctggtaaaac
     9961 ctcgagctgc caaaccagcg gtccagcctg gccggtggaa cggcgtcttc gcgatgccca
    10021 tgggtcgcgc cttcgtgtcc atgggcatgg ctgtgcacgt aatcgccgtg gcgatgcggg
    10081 tgctgatgaa ctccatcgtg gtcgcggccc gcgctcgcgc tctcgaccgc gcgaaaagtc
    10141 gcgttcaggt tcaatgcccc gagcaggatc aacatcaggg ccacgcaaaa ttccaaactc
    10201 atccccagcc gttccggaac gacgatcccg aagaggatga tggcgccgcc gatgacgagc
    10261 agcgtcaggc tgtgtccgat gccccagaaa attcccgtcc aggccgcgct cccaatgtgg
    10321 cgctgccggc tcacgatggt cgtgaccgcg atgacatgat cggaatcggt cgcgtgccgc
    10381 atgccgagaa aaaaaccgaa gagcaggatc gggaagagtg tcctcatgag agggcgccgg
    10441 caatttcgtt aggcataaat cggaccgagc ggatggcgca ttcttcgccc tcgatgacgc
    10501 gcgctgtttt gccgcagccg gggcactcga gggtggccac gggcacccca tgcagggcgc
    10561 cgtcccaata tttcggcagt ccgctgtagg agcaatccgg gcaggcgact ttgcccagga
    10621 ccggctcgat cgtcaggcgg gaaccggcga tcggcatctc ggtgatgatc gactcgtagc
    10681 agaagcgcag ttgctcctcc tcgatctggg tgaactcgcc gatcgcgacc cgaacttcga
    10741 caattttctt gtccggattt ttctccgcaa aatcgagcag cttttcgacc aacccggacg
    10801 cgatcgaaaa ctcatgcatg tggaggaaat gactaatgac caattacgaa tgacgaatta
    10861 atgaccaacg acgaagcccg aatttggtca ttcgtatttc gggtttcctt cgtcattggg
    10921 catttgaaat tcgtcattcc tcaaaccgcg agcaaagttg ccgcgacttc gtccagaccg
    10981 gtgccgttct tgcaactcgt ggggataacc ttgatgtccg gtttgagtac atggacgtcg
    11041 ttggtcaact gctcgacgct cacttccatc gcttccgcga gatcgatctt gttgatcaca
    11101 acgagctgcg cgccgaggaa catgtgcgga tgtttccgca ccatccaggg gccttctgtc
    11161 acgctcacga cgaccacgcg ggccttcgag ccgagcggaa attccgccgg gcaaatgagg
    11221 ttcccgatgt tctcgatgaa gatcagcttc aggttgtcga gcttgatctt gcgcaggcct
    11281 ttgccgacga ggttggcatc gagatgacag ccattgatcg tcgcgatttg gacgaccggc
    11341 acattctgtt tcgagatcgc gtcggcgtca ttgctcgtgg tcgcgtctcc gttgaaaacc
    11401 gcgacgccca tccggtcctt taacctctcg accagcttcg tgataagtgt cgtcttgccg
    11461 gaaccgattg cgcccatgaa gtcgatcgcg gtgattccgc tccggtcgag cagcgcccga
    11521 ttctcttgcg ccagcttggc gttcgcctcg agcagactgt gctccagttc gatgtcgtag
    11581 atctcgtccg gtgacgcttc gagcatcacc tgcgctgcgc tttcctgggc catgatcagc
    11641 gagccaggcc ggcgagagcg gtcggagtgt gcatctgctt cagcaccttc ccgccgccga
    11701 gatacatgtg gacgccgcag ggcagacaag gatcgaagct gcgcaccgcg cgcatgatat
    11761 caatgccctt gaagttgtcc gggccgttct cctcgaagat cggagtgttt tggaccgcgt
    11821 cttcgtaagg cccaggcgtg ccgtaaatgt cgcgcggatt cgcgttccac ggcgtcggcg
    11881 gatacggatg gtagttggcg atcttgcctt tgcggataac catgtgatgc gacaacacac
    11941 cgcgcaccgc ttcgtggaaa ccgcagccga tggcttcatc tggcactttg aatggggtga
    12001 aggtcgcggt ccgtccggca tgcacttccg ccatcgcctt ctcgaggaag taaagggcgc
    12061 agcaggcggc gtaaacctgg aagtaagtgc gcgcgcggtc gcgttcgatc gcgttgctcc
    12121 atttcggcac tttccattcg aactcgactt cgcctttcaa cgcggtcttc gggagacgca
    12181 tcttcacgct gtggccggtc gcctcgacgt agccaatgtt aaccagcttc gcgagcgccg
    12241 tcgcccagag acgtgcaatc gggccgccgc cagtgtccat cgccagatga tcgccggtcc
    12301 gcttgtcgag ccagcgcgga gacatgaccc aggtgtagtt gcccccgaag tcgcgccgtt
    12361 gcggcttcgg aatcgtagtc tgattccagg gatgcttttt gtcgaccgga ttgcctaacg
    12421 gatcgttttt gacgaaggtc tcggagttat cccaatcgtc gtagtaggac gaaccgagca
    12481 ggatgcgaat gccgaggttg atatcgacga ggttggtggt gaccagctcg ccgtcgatca
    12541 ccatgccggg cgtgacaaac atggcgttgc cccacttttc catcgttttg tagtcgtaat
    12601 cgcaaacgtc tggatcctgg aacgatcccc agcaggcggt caggatgcgc cggcggccga
    12661 cttcttcata acccggcagc gcctcgtaga agaaatcgaa gagatcgtca tgcagtggca
    12721 ggcaccgttt catgaactcg atgtaacgca tcaagcggac ggtgtagtcg gtgaagatct
    12781 ggatcgatgg gacggtcccg accccgccgg gatagagcgt cgagggatgg acgtggcgtc
    12841 cttccatcaa gcagaacatc tcgcgcgtgt agcgactgac gtgcagggtc tcgcgataga
    12901 attcaccgga gaacggatta agcgctgtca tgatgtcggc gattgttcga tagccgtgat
    12961 cattggagtg cggtgcgagc gtcgtctgcg ccttggccca gacgctcggg ttggtctcct
    13021 tcaccatcat ttcgcaaaag tcgacgccga cgaggttatc ctggaagata ttatgatcga
    13081 acatgtactc ggccgcttcg ccgaggttga cgatccattc ggccagggcc ggcggtttga
    13141 cgccgtaggc catattctgc gtgtagaccg aacaggtcgc atggttatcg ccgcagatgc
    13201 cgcagatccg gctggtgatg aagtgggcat cacgcggatc tttgttctgc atgaagatgc
    13261 tgtagccgcg gaagatcgat gaggtgctgt agcactcgac gactttcttc gagtcgaaat
    13321 cgatcttggt atagatgccg aggctgccga cgatccgggt aataggatcc cacgacatct
    13381 cgacaatgtt accggatggg gcggaggatt tggtggcggg tgcggttagg atatcaggca
    13441 ttttagtggt ccagttgagt tagcgatttc gaagtcccat tttagttgcg cgcgtaattt
    13501 cttggctcat agccggttgt gagttccgcc cgcggatgac gccatttcgg ttccttgttg
    13561 acggtcgtgt tggtgatggc gcgcagcttg cggagcgcgc cgccccaaag acctacgacc
    13621 ttggtcgaca aggaggcccc cggaggcgca tccataaacg gcatgaactt gtcggggaat
    13681 cccggcatgg tgcagccgat gcagattcca cccacgttag ggcaaccacc gatcccggcc
    13741 atccagccgc gcttggttac gttacagttc accaccggac cccagcaacc gattttgacg
    13801 aggcatttcg gtgaaccata ctcatgggcg aaatcgccct gctcatagta accggcgcgg
    13861 tcgcaacctt cgtggaccgt cttgccgaag agccaggtgg gtctaagttg ttcgtccagc
    13921 gggatgagcg gagccagacc cgcgacctga taaaggagat agagcagtgt ctccatcatg
    13981 ttatccggtt ggaccgggca gcccggcaca ttcacgatcg gcaggccggc tttcgaacgc
    14041 cagccccagc cgagatagtc agccagaccc atcgcgcccg tcggattgcc ttccatggca
    14101 tgaatgccgc cgtaggtggc acaggttccg caagccacaa ttcccagggc tttcggagcg
    14161 agccgatcga tccactcatt ggtcgtaatc ggctgccccg tgtcggggtc ggtgccgaga
    14221 gcggcccaat agccttcctt cttaatcttc tcgttcggga ttgagccttc catgacgaga
    14281 acgaacgggt cgagcttgcc agcggcggca tcgaaccagt gtttcatgaa ttcgtcgccg
    14341 acctcgtagg caatcaccgg attgtgcaca tgcactttgg gcagcccggg aatattgccc
    14401 atgaccagat cctcgatgct gggttgcgtc gcggccgtga tcgacacgga gtcaccgtca
    14461 caacccaatc ccgtcgtcat ccagatgaca tgaatttcct taaccgccga ttcccgatgc
    14521 ttgaagggtg tgttagctgc taccataggt cttgttctcc ccggcggcgc gatccggatt
    14581 cattcccggt cgggtcgcta ctttgttcaa ttgcgactta catactgaaa aatggtgatg
    14641 aaattgcgaa aaaaattaca tgtcgcgcaa ccgaaagtag cgccgcagct ccggaatcgc
    14701 ggagaggaat gagaccacgg cgaaaaggac gattccgcct aacaatacca gccagcaaat
    14761 ttctgctgta ttcatgtctt cttctttcat tcggttttat tcaaggaatc gtttcacact
    14821 cgcgaatcga ttgtggcgac cgtgcttccg gtccctttaa aatattgcca atgagcgact
    14881 cgatcagctc gatcgcgggc tggaccgcgg cctgaacctt ttcgctcaat cccatcgcgc
    14941 cttcctcggc ctcgagaatt gccggctcac aaccaaccac ccgtaaccac cccggcgcac
    15001 cgcctaacga gcggaccagt tgcagaacgc gaaccggatt catgctatga gcatttaccg
    15061 cctcgtcggg taactcgtcc agtttttttt gatcgggttc gatcaaataa atggtgcccg
    15121 gcgcgctgcc ttgtggactg gcatccac
//
LOCUS       DATMNS010000060         3055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_436149, whole genome shotgun sequence.
ACCESSION   DATMNS010000060 DATMNS010000000
VERSION     DATMNS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3055
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436149"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..912)
                     /gene="sucD"
                     /locus_tag="VNW28_02370"
     CDS             complement(31..912)
                     /gene="sucD"
                     /locus_tag="VNW28_02370"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXA08796.1"
                     /translation="MSVLVDKNTRLCVQGITGSAGGFHARQCMEYGTNVVAGVTPGKG
                     GQKFENKVPVFDTVWEARQATSCNVSVLFVPAAGAADGILEAVDAGVELVICITEGIP
                     VIDMMRVKAEMAGKKSWLIGPNCPGIITPGECKIGIMPGYIHKPGNVGVISRSGTLTY
                     EAVWQLTSRGYGQSTCIGIGGDPINGMSHLDAVKLFNDDPKTEAMILIGEIGGAAEEE
                     AAAWIKDNCRKPVAAFIAGATAPAGRRMGHAGAIVSGGKGTAAGKIEALKAAGIAVAD
                     TPATMADTLITTMNRRR"
     gene            complement(1048..1401)
                     /locus_tag="VNW28_02375"
     CDS             complement(1048..1401)
                     /locus_tag="VNW28_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXA08797.1"
                     /translation="MIKEVAFVAIAVSDKERARNFYQETLELKPGMTAMEGAWIEYDL
                     GATTIGVGCWPGWDPSRDGTSIAFEVDDIDSTIATLKERGVVFDMEKTETPVCWMAQF
                     RDPDGNKLMVHKRKK"
     gene            complement(1401..1781)
                     /locus_tag="VNW28_02380"
     CDS             complement(1401..1781)
                     /locus_tag="VNW28_02380"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXA08798.1"
                     /translation="MKAKKIAFTGIPVTDLKRARAFYEGVLGLKPSAEFNQGVWIEYE
                     IGPDTLAIGNVGDQWKPSEDGTSVAIEVEDFEAAIKSLKDAKADIAAEGIESPCCRMA
                     VVRDPDGNKLIIHKLKPENEKGPC"
     gene            complement(1778..2968)
                     /gene="sucC"
                     /locus_tag="VNW28_02385"
     CDS             complement(1778..2968)
                     /gene="sucC"
                     /locus_tag="VNW28_02385"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HXA08799.1"
                     /translation="MNIHEYQAKELLQKFGAATPGGKVATTAEEAEQIARELGTNDLV
                     VKAQVHAGGRGKGTFANGFKGGVHLVKSPAEAREVAGKMLGQTLVTHQTGPAGRVVNK
                     VLVAVSVEIEREIYFAVLLDRATAAPLIVASTEGGVEIETVAEKSPEKIMREPIDPLA
                     GLQPFQTRKLAKQLGFKSDQIKPAAKLFAALYRTFVESDCSMVEVNPLVVTKNGEVLA
                     LDAKFNFDDNALYRHPEIAAMRDTAEEDPREVEASKHGLNYIGLDGNIACLVNGAGLA
                     MATMDIIKFYGGRPANFLDVGGGATEEQVTEAFKLLISDENVKAILVNIFGGIMKCDV
                     IAQGIINAVKAVRLPVPLVVRLEGTNVDAGKKLIAQSGLAVIAADDLADAAQKVVKAA
                     QQQR"
BASE COUNT          530 a          973 c          841 g          711 t
ORIGIN      
        1 tccagacctt tggcggtaac gggcatcggc ttatctcctc ctattcatcg ttgtaatcaa
       61 ggtgtccgcc atcgtcgccg gcgtatccgc gaccgcgatg cccgcggcct tgagcgcttc
      121 gatcttgccc gcggctgttc cctttccacc ggaaacgatg gcgcctgcgt ggcccatgcg
      181 gcgcccggca ggcgcggtcg ctccggcgat gaaggcggcg accggtttcc ggcagttatc
      241 cttgatccag gcagcagctt cctcttccgc cgcgccgccg atctcgccga tcaaaatcat
      301 cgcttcggtc tttggatcgt cgttgaaaag tttcaccgcg tcgagatgcg acatgccgtt
      361 gatcggatcg ccgccaatgc cgatgcaagt cgactggccg tagccacgcg atgtgagttg
      421 ccagaccgct tcgtaggtca gggtgccgct gcgcgagatg actccgacgt tgcccggctt
      481 gtgaatgtag ccaggcatga tgccgatttt gcattcgcct ggcgtgatga tgcccgggca
      541 attcggcccg ataagccagg attttttgcc ggccatttcc gctttcaccc gcatcatgtc
      601 gatcactgga atgccttcgg tgatgcaaat gaccagctcg acgccagcat caaccgcttc
      661 caaaatccca tcagccgcgc ccgcggccgg aacgaagagc accgaaacgt tgcaactcgt
      721 cgcctggcgc gcttcccaga ccgtgtcgaa aaccggcacc ttgttttcaa atttctgtcc
      781 gccttttccc ggcgtgaccc cggccacgac gttggtgccg tattccatgc actgcctggc
      841 gtggaatccg cctgcgctgc cggtaatccc ctgcacgcag agccgggtgt ttttatcgac
      901 gaggactgac atgggtgaaa tgacgaatgt cgaatgtcga atgtcgaagg aatgaccaag
      961 gtcgaatgac gaaatgcgga ccgcgatagc cgttcttttc ggatttcgtc attcggattt
     1021 ccctcgtcat tcggcattcg taattcgtca tttttttcgc ttatgaacca tcagcttgtt
     1081 cccgtccgga tcgcggaatt gcgccatcca gcaaacgggc gtttccgttt tttccatgtc
     1141 gaatacgacg ccacgctcct tcagagttgc aatcgttgaa tcgatgtcgt ccacctcgaa
     1201 agcgatcgat gtcccgtcgc gcgatggatc ccagccgggc cagcagccga cgccgatcgt
     1261 ggtcgcgccg aggtcgtatt cgatccatgc gccttccatc gccgtcatgc cgggcttcaa
     1321 ctccagcgtc tcctggtaaa aattccgcgc gcgttccttg tcactcaccg cgatggctac
     1381 gaatgcaacc tccttgatca tcagcagggt cctttctcgt tttctggttt tagtttgtga
     1441 ataattagct tgttgccatc cggatcccgc acgaccgcca tgcgacagca cggactttct
     1501 atcccctcgg cagcaatatc tgctttcgcg tctttcaggc ttttgatcgc cgcctcgaaa
     1561 tcctcgactt caatggccac actcgtccca tcctcggatg gcttccactg gtcgccgacg
     1621 ttgccgatcg cgagggtgtc ggggccgatc tcgtattcaa tccagacccc ttggttgaac
     1681 tcagccgacg gcttgagccc aagcacgcct tcgtagaaag cgcgcgcgcg tttgaggtcg
     1741 gtcacgggaa tgccggtgaa ggcgatcttc tttgctttca tcgttgttgc tgcgcggctt
     1801 tgacgacttt ctgcgcggcg tcggcgagat cgtccgcggc gatgaccgcg agaccgctct
     1861 gcgcgattaa ttttttcccg gcatcgacgt tggtcccttc cagcctaacg accagcggga
     1921 ccggcaacct gaccgccttg accgcgttga tgatcccctg tgcgataacg tcgcacttca
     1981 tgatcccccc gaagatgttg acaaggatcg ccttcacgtt ctcgtcggaa atgaggagct
     2041 tgaacgcttc cgtcacctgc tcctcggtcg cgccgccgcc gacatcgagg aagttggccg
     2101 gcctgccgcc gtaaaacttg atgatgtcca ttgtcgccat ggcgaggccg gcgccgttca
     2161 caaggcaggc aatgttaccg tcgagcccga tgtagttgag cccgtgcttc gaggcctcga
     2221 cttcgcgcgg atcttcctcc gccgtgtcgc gcatcgcggc gatctccgga tgccggtaga
     2281 gcgcgttgtc gtcgaagttg aatttcgcgt cgagcgccag cacttctccg ttcttcgtca
     2341 cgacgagcgg attcacttcc accatcgagc aatccgactc gacgaaggtg cgataaagcg
     2401 cggcgaaaag tttcgcggcc ggtttgattt gatccgattt gaagccgagt tgcttcgcca
     2461 gtttgcgcgt ctggaagggt tgcaacccgg ccagcggatc gatcggttcg cgcatgattt
     2521 tctccggcga tttctcggcc acggtttcga tttccacgcc gccttcggtg ctcgcgacga
     2581 tcaagggcgc cgcggtcgcg cgatcgagca acacggcaaa ataaatctcc cgctcgatct
     2641 ccacggaaac cgcgaccagc accttgttca ctacgcgccc ggccggaccg gtctggtggg
     2701 tgacgagcgt ttggccgagc attttcccgg cgacttcccg cgcttccgcg ggtgacttca
     2761 caaggtgcac gccacctttg aaaccgttcg cgaaagtccc ctttccgcgc ccgccggcat
     2821 gcacctgcgc tttcacgacg agatcgttcg tgccgagttc gcgcgcgatc tgttccgctt
     2881 cctctgccgt ggtcgcaacc ttcccgcccg gggtcgcagc accgaacttt tgcagcaatt
     2941 ccttcgcctg atattcatga atgttcatgc gcgggacggt tggacagacg agcaattagc
     3001 tcgcgaaacg caggccagca acggaatttt ccccgtcgga ttcgccgctc acggc
//
LOCUS       DATMNS010000061         4010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1832816, whole genome shotgun sequence.
ACCESSION   DATMNS010000061 DATMNS010000000
VERSION     DATMNS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1832816"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..921)
                     /locus_tag="VNW28_02390"
     CDS             complement(208..921)
                     /locus_tag="VNW28_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXA08800.1"
                     /translation="MRQNQRAAFVTGANKGIGFEVARQLVREGFRVFLGARDRKAGKV
                     AAEKLNQEGKNQGKISFLEIDVSQPESVQRATEEFARQSDRLDALVNNAGILLDEDKD
                     VLALSAEMFQTTLRTNTLGALLVSQAFLPFLRKSDNPRIVNVSSGGGQLTDGADGWAP
                     AYCISKTALNGVTVQLAAALPDFAVNSVCPGWVRTDMGGANATRSVVEGASGIVWLAA
                     DAPQGETGKFWRDRKVIPW"
     gene            complement(1202..1528)
                     /locus_tag="VNW28_02395"
     CDS             complement(1202..1528)
                     /locus_tag="VNW28_02395"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HXA08801.1"
                     /translation="MSGIIVIVAYKPKPGKTSEEVLALVRRRMPLLRGEGLVTERVPV
                     IMRCRDGTIIEVSEWKSQAAIDAAHKNPNVLAFWGEMFAVIDCVPLKTLPEAEDMFAG
                     FEPLEE"
     gene            complement(1716..2024)
                     /locus_tag="VNW28_02400"
     CDS             complement(1716..2024)
                     /locus_tag="VNW28_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08802.1"
                     /translation="MKPTSIFTTEHRLARHDRRAESRRVRPATDYFFRSPPDATLGRS
                     RHRPSRRSVRAFRRMMAEMRIKHGRDEPMELFLFGFVAALAAWPLVDLVIVLAQTANG
                     "
     gene            2509..2943
                     /locus_tag="VNW28_02405"
     CDS             2509..2943
                     /locus_tag="VNW28_02405"
                     /inference="COORDINATES: protein motif:HMM:NF039485.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HXA08803.1"
                     /translation="MGLLDSLLGDAAGKATAAGGTEAAVAAVIGTVLTQNGGVQGLMN
                     KFSQGGLGGVFSSWVSTGENQAVSASQLQNVLGSEQVQALAAKLGVDPATASGLLAQF
                     LPKVVDKLTPTGQVDPNADHQQGLAALLPSLLQELGGQSHQP"
     gene            complement(3108..>4010)
                     /locus_tag="VNW28_02410"
     CDS             complement(3108..>4010)
                     /locus_tag="VNW28_02410"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HXA08804.1"
                     /translation="DGYGVVEMKQPVENEFFMEGSLNGHPIRLILDTGFCSEEITMKN
                     AAAVYLHSPLQPIKDVGHSLSGKAIGHQSKGIADSLVLGNVQVSGVTLHFASLPSLEG
                     HAQGGLWFTDGMLDNARAVRNDADGFLGLGFLRICAAIIDPANKRLYLKPPRTGRTPQ
                     LGPALKPLGFSEAPFQVTGYGLLVDVSINGVSGKMIIDTGDDITLVESHFAARAGLKT
                     YRSGGRTMTDAAGTKVEPEWADPASFRIGGLETYRPKVQVQPVPFLLPTGGRVVGLLG
                     MDFIGQSWGIIDFAQGKLYFTVTK"
BASE COUNT          802 a         1179 c         1189 g          840 t
ORIGIN      
        1 gatggtgagg gggatttgcg gcgtggtttc tttgatcatg tttcctcctt tgataatttg
       61 gttgtcggcc aaaaccgaac gtcattcaat tcggccggcg gtttgtgaac ggctgacttc
      121 cttgcgcccg cctctaaagc aggcgggtct attaagttcg tgccgaatga ctcggccgga
      181 cttccgcgtt gcctcagcgt atcgagatca ccacgggatc actttgcggt ctcgccaaaa
      241 ctttcccgtt tcgccttgtg gcgcgtccgc cgcgagccag acaattccgg atgcaccttc
      301 gaccacggaa cgggtcgcgt ttgcgccacc catatctgtt cggacccagc cgggacagac
      361 ggaattgacc gcaaaatctg ggagggcggc ggcgagttgg acggtgacgc cgttcagcgc
      421 tgttttcgaa atgcagtagg cgggcgccca accgtcggcg ccgtcggtca gttgtccgcc
      481 gccgctggaa acattgacga tccgcggatt gtcgctcttt ctcaggaagg gcaggaaagc
      541 ctgcgagacg aggagcgccc cgagcgtatt ggttcgcagc gtcgtctgga acatttccgc
      601 gctgagcgcg agcacatcct tgtcttcatc gaggagaatg ccggcgttgt ttaccagtgc
      661 gtcgaggcga tcgctttgcc tggcaaattc ttcggttgcg cgctggacgc tttccggctg
      721 ggagacgtcg atctcaagaa aggaaatctt tccctgattt tttccctcct ggttgagctt
      781 ttccgccgcc accttgccgg cctttcgatc gcgcgcgccg agaaacaccc gaaaaccttc
      841 gcgcacaagt tggcgcgcca cttcgaaccc gatcccttta tttgcgccgg tcacgaaggc
      901 ggctctttgg ttttgtcgca ttcacgaaat tgcgacgtct gcctttggaa atcaaaaccc
      961 caaatgagca atccgtcgcc gtttgtcggt gaccatgaac cgaccgacat cgcaggggcc
     1021 gcgtattaat gtgtcgtcgt cgatttccat tcgccgcgga gtgaatcaga atccgttttt
     1081 tgaccaggtt tcggtccacg cttccaattc gcacgagcag ggcattcggc gagtcaccga
     1141 atgcgacacg cgagtcgcgt atgctcccca acctgatttc tgagggccgc agcctaacga
     1201 atcattcctc cagcggctcg aagccggcga acatgtcctc cgcttcgggg agagtcttga
     1261 gcgggacgca atcgatcacg gcgaacatct cgccccagaa ggcgagaacg ttgggattct
     1321 tgtgcgcggc gtcgatcgcg gcctgcgatt tccattcgga gacttcgatg atggtgccgt
     1381 cgcgacaacg catgatcacg ggcacgcgct cggtcacgag tccttcgccg cgcaggagcg
     1441 gcatgcgccg ccggacgagc gccagaacct cctcgcttgt tttgccgggc ttcggtttgt
     1501 aagcgacgat cacgattatt ccgctcatga ccgcattcta gcgcgatcgc attttccggc
     1561 aacggcacga tttgcttcgg agacgcgcaa ttcgctggga cccgcctcgt tggtttaaca
     1621 tgtgcacgct gtgacggccg gctaacggca ggcgacgcgc ctgccctaca ggcgatttgt
     1681 tgcgttcctg ctcttgcgag atcatccagc gccgatcacc cgttggcggt ctgggccaga
     1741 acgatgacga gatcgaccag cggccaggcg gcaagcgcgg cgacgaaacc aaaaaggaac
     1801 aactccatgg gttcgtctcg tccgtgtttg atccgcattt ccgccatcat cctgcgaaac
     1861 gcgcggactg accgccggct ggggcgatgc cgggagcggc cgagcgttgc gtccggtggc
     1921 gaccggaaga aatagtcggt cgccgggcgg acgcgacgac tttctgctcg acggtcgtgc
     1981 cgggcgagcc ggtgttcggt agtaaagata gaggttggct tcatactgtt ttggtgagtt
     2041 gtggtttccg agagtttcga gttctcttca ctggaacgaa ggaagaacgc tccgcaggac
     2101 aaaggttggg ccagagaatt cgccgcgggc gatccggtgg tcgaccccga aatgcttcgg
     2161 ggcaggtcag acgccgatcg cagactgccg tagccgggcg ttctgaatcg aagtggtggt
     2221 ttgcatgata aggaaaacta aaccggccgg gcgggctgtc gacttccgcc gcgttacatt
     2281 caggtgactt tctcctgcga gcgccccggg aagttgccca aggggctctc atcaagtgcg
     2341 ccggaaacag ctaactcgag atcgaaacgc ggaaaggaac aaggaagggg gattaggaat
     2401 tgcgcaaagg aggcctgaaa aacgccggct tccaatgctc gataatttcg gcgacaaacc
     2461 gcgcggccgc gactagcgtc gctggaaaac caactggaga agaacattat gggattactc
     2521 gattcacttc tcggcgatgc cgccggaaaa gcgacagcgg ccggcggcac agaagcggcg
     2581 gtcgccgccg tgattggcac ggtgctgacc caaaatggcg gtgtccaagg gctgatgaac
     2641 aaattctcgc aaggcggcct cggcggggtt ttctcgtcct gggtcagtac gggggaaaac
     2701 caggccgtct ccgcgtcgca gcttcaaaac gtgctcggct ccgaacaggt ccaggcgttg
     2761 gctgcgaagc ttggggttga tcccgcgacc gcgtcgggat tgcttgccca attcctgcca
     2821 aaggtcgtgg ataaattgac acccaccggc caggtcgatc cgaacgcgga tcatcaacaa
     2881 ggcctcgcag cgctcttgcc ctcactcctc caggaactcg gcggtcagag tcatcagccc
     2941 tgacggatac ttcctggttc gcgacaaaaa aacctggctt taaaggagcc tgtccgccaa
     3001 cgggggacgc tcgctgattc tgaaaatcaa gacgagcgta gaaacgaacc tcgcggagcc
     3061 agtcgcttcg cttcgcggcc acgaactcgt ttactcgtta ggcaacgcta cttcgtcacc
     3121 gtgaagtaaa gcttgccctg ggcaaagtcg atgatgcccc agctttgccc gatgaagtcc
     3181 atgccgagca aaccgaccac tcggcctccc gtgggcagaa gaaaaggtac gggttgcacc
     3241 tgcactttgg gacgataggt ctccaatccg ccgatcctga aactggcggg gtcggcccat
     3301 tccggttcga ctttcgttcc cgcagcgtcg gtcattgtcc tgccccccga ccggtaggtt
     3361 ttcagccccg cccgcgccgc aaagtgactt tccaccaggg tgatgtcatc cccggtgtca
     3421 ataatcatct tgcctgaaac accgttgatc gagacgtcaa cgagcaatcc ataccccgtc
     3481 acctggaacg gagcctcgga aaaacccagg ggtttgagcg ccggtccgag ttggggagtt
     3541 cgcccggtcc tcggaggctt cagataaaga cgcttattgg cgggatcaat aatcgcggcg
     3601 caaatgcgca agaaccccag gccgagaaac ccgtccgcat cgttcctcac cgctcgcgcg
     3661 ttgtcgagca tgccgtccgt aaaccaaagg cccccctgag cgtgtccctc cagggatgga
     3721 agtgacgcga aatgaagagt gactcctgag acctgaacat ttccgagcac cagcgagtcg
     3781 gcgattccct tgctctgatg cccgatggct ttgccgctca ggctgtgccc gacgtctttg
     3841 attggttgca gtgggctgtg caaatacact gcggccgcgt ttttcatcgt aatctcttcg
     3901 gagcaaaagc cggtgtccaa aatgaggcgg atcgggtggc cgttgagaga tccttccatg
     3961 aaaaattcgt tttccaccgg ctgtttcatc tcgacgacgc cgtagccatc
//
LOCUS       DATMNS010000062         3442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3262566, whole genome shotgun sequence.
ACCESSION   DATMNS010000062 DATMNS010000000
VERSION     DATMNS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3442
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3262566"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="VNW28_02415"
     CDS             complement(<1..120)
                     /locus_tag="VNW28_02415"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HXA08805.1"
                     /translation="MPKVPITMPQLGESMAEATIVAIKVQPGEKVVADQEIIEV"
     gene            complement(147..1115)
                     /locus_tag="VNW28_02420"
     CDS             complement(147..1115)
                     /locus_tag="VNW28_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXA08806.1"
                     /translation="MSVTYLEAIREAQAHLLREDERVFIYGQDVSGKFGGAFKATKGL
                     AAEFPGRVLNTPISEDAMVGTAIGAAMEGMRPIVEMQFADFSSIALNQILNNAGTQYW
                     RTNIPCPITVRLPSGGTKGSGPFHSQSMEAIYSHYPGLVVVAPATVEDAYTMLIDAVA
                     IDDPVIYCEHKYLYYHLKADALPKDRLPIGKARIVRPGRDLSIVTYSAMVHEALAVAK
                     EFSASGVEIEVVDLRSVKPLDTDTVLASVARTGRLLCVGESFPWGGATAEIIARVATE
                     GFHLLDAAPARINARDTPIPYHPNLWSDHRPNAKSIAAKVRQVLTE"
     gene            complement(1248..2282)
                     /locus_tag="VNW28_02425"
     CDS             complement(1248..2282)
                     /locus_tag="VNW28_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXA08807.1"
                     /translation="MLQTAKRAADSASQKAVPHERYLEAFRWMLQTRIFEDKLASLYR
                     GGLITGGVYIGRGQEAVSTACGLFLQKGDVLAPLIRDQAARATFGEPLLNVTRTYLGS
                     RQGSMRGRDGNIHRGYPAAGQLAMISHLGAMIPVVVGKLLAKRMKGEKGFVGLTMIGE
                     GGMQTGATHEGMNIAAVEKVPFVLVASNNHFAYSTPNDREFACAHLVDRARGYGYEGQ
                     TVEGTDLAACLEVIGGAVERARAGGPPQLVVAQLLRLAGHGEHDDASYVPPEMKKERW
                     ALDCIALTEQLTLKNNWLDAKMLKQWRAEISAEVEKAVETAQREAAPVGSEEDWCALS
                     TRELVDVVGE"
     gene            complement(2430..3185)
                     /locus_tag="VNW28_02430"
     CDS             complement(2430..3185)
                     /locus_tag="VNW28_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HXA08808.1"
                     /translation="MNFKLKIWRQTAADSKGKLKPYEISDISPATSFLEMLDILNEQL
                     TTRGEEPIAFDSDCREGICGTCSLTIDGEAHGPSHPAAACQLYLREFKDGATITIEPF
                     RARSFPIVKDLVIDRSALDRIVQAGGFVSARTGSAPEANSIPVPKHKADRAMEAAACI
                     GCGACAAACPNASAMLFTSAKISHLALLPQGQPERRRRVLRMVRQMDAEGFGNCTNTY
                     ECEAVCPAEISVSFIAKMNREYAVASLGERLGE"
     gene            complement(3315..>3442)
                     /locus_tag="VNW28_02435"
     CDS             complement(3315..>3442)
                     /locus_tag="VNW28_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08809.1"
                     /translation="RDDENFADVFAWEYQGDGTEPNLHTEPLVFESVHLATRSYK"
BASE COUNT          604 a         1083 c         1067 g          688 t
ORIGIN      
        1 gacttcgatg atttcctggt cggccacgac tttctcgccc ggctgtacct tgatcgcgac
       61 aatcgtcgcc tcggccatcg attcgccgag ctgcggcatg gtaattggaa ctttcggcat
      121 tggagaaatt gagagttgag agatggctac tcggtgagga cctggcggac tttcgcggca
      181 atcgacttcg cgttcggtcg atggtcgctc cagaggttcg gatggtaggg aatcggcgtg
      241 tccctggcgt tgatgcgcgc gggcgcggcg tcgagcagat ggaacccttc ggtcgcgacg
      301 cgcgcgataa tctcagccgt cgcgccgccc cacgggaacg attctcccac gcaaagcagc
      361 cgccccgtcc gcgcgaccga ggcgagcaca gtatcggtat cgagcggctt cacgcttcgc
      421 aggtcgacca cttcgatctc gacgccgctg gcgctgaatt ccttcgcgac cgcgagcgct
      481 tcgtgcacca tcgcgctgta ggtcacgatg ctcaggtcgc gtcccggcct aacgatccgc
      541 gctttcccga ttggcagccg gtccttcggc agcgcgtccg ctttgagatg gtaataaaga
      601 tacttgtgct cgcagtagat gaccgggtca tcgatcgcga ccgcatcgat cagcatcgta
      661 taggcgtctt ccaccgttgc gggcgcgacc acgacgagcc ccgggtagtg cgagtaaatc
      721 gcttccatgc tctggctgtg aaacggtccg ctgcccttcg tgccgccgga gggcagacgc
      781 accgtgatcg gacacggaat attcgtccgc caatactgcg tgccggcgtt gttcagaatc
      841 tggttgagcg cgatgctcga gaaatcggcg aactgcattt cgacaattgg ccgcatccct
      901 tccatggccg cgccgatcgc cgtcccgacc atcgcgtctt cgctgatcgg cgtgttcagg
      961 acgcggcccg gaaattctgc ggcgagaccc ttcgtggctt tgaacgcgcc accgaacttt
     1021 ccgctcacgt cctgcccgta aatgaaaacg cgctcgtctt cccgcaggag atgcgcctgc
     1081 gcttcgcgaa tggcctcgag gtaggtgacg ctcatgggaa cactgacgaa tgacgaatgt
     1141 cgaatgacga aggaatgatc aaggacgaat gccgaaacgc cagcgcgcgc gatcggtctt
     1201 ggacttcgga cttcgtcatt gattcggatt tcgtcattcg caattcgtca ttcccccacc
     1261 acatccacca attcgcgcgt tgacagcgcg caccaatctt cttcactccc gaccggcgcc
     1321 gcttcgcgct gggccgtctc gaccgccttc tccacctcgg cggaaatttc cgcgcgccat
     1381 tgcttgagca ttttggcatc gagccagttg ttcttgaggg tcaactgctc ggtgagcgcg
     1441 atacaatcca acgcccagcg ttccttcttc atctccggcg gaacataact ggcgtcgtcg
     1501 tgttcgccgt gacccgcgag gcgcaaaagc tgggccacga ccagttgcgg agggccgccg
     1561 gcgcgcgcgc gttccaccgc gccgccgatc acctcgaggc acgccgctaa atcggtgcct
     1621 tcgactgtct gcccttcgta gccgtagccg cgcgcgcggt cgaccaggtg ggcgcaggca
     1681 aattcacgat cgttcggcgt ggagtaggcg aaatgattgt tcgacgcgac gagaacgaac
     1741 ggcacctttt ccaccgcggc gatgttcatt ccttcatgcg ttgcgccggt ctgcatgccg
     1801 ccttcgccga tcatcgttag gccaacgaac cccttctcgc ccttcatccg cttggcgagc
     1861 agcttgccga ccacgaccgg aatcatcgcg ccgagatggc tgatcatggc gagttgcccg
     1921 gccgcgggat aaccgcggtg gatgttgcca tcgcgcccgc gcatcgaccc ctggcgcgag
     1981 ccgagatagg tgcgcgtcac atttaggagt ggttcgccaa acgtggcgcg agccgcctgg
     2041 tcgcggatga gcggcgccaa cacgtcgccc ttctgcaaaa acaaaccaca cgccgtgctc
     2101 actgcttcct gcccgcgccc gatgtaaacg ccgccagtga tcaggccgcc gcggtagaga
     2161 ctggccagtt tgtcctcgaa gatccgcgtc tggagcatcc agcgaaaagc ctccagataa
     2221 cgctcatgcg gcacggcctt ctgcgaagcg gagtcggcgg cacgtttcgc ggtttgcagc
     2281 atcggaggac ttaaaccagc aacgtgcggc acgccaaaat tttttgttta ggcgccgcga
     2341 gggcaccttt cgcgggtctc ctgtctcagc ggccgggaga ttgtccgctg gtggggccgc
     2401 ctcgccccgg acaccgcgcc gaacccgcgc tactcgccta accgctcgcc caggctggcc
     2461 acggcgtatt cgcgattcat tttcgcgatg aagctgacgc tgatctcggc ggggcagacc
     2521 gcttcgcatt cataagtgtt ggtgcagttg ccgaagccct ccgcgtccat ctggcgcacc
     2581 atccggagaa cgcgccgtct ccgttccggt tggccctgag gcaggagcgc gaggtgggaa
     2641 attttggcgg aggtgaagag catcgcggaa gcattcgggc aagccgccgc gcaggccccg
     2701 caaccgatgc aggcggccgc ttccatcgcg cggtcggctt tgtgtttcgg gacgggaatg
     2761 gagttggctt ccggcgcgga accggtgcgc gcgctgacga acccgcccgc ctgcacgatg
     2821 cgatcgagcg cggagcgatc gatgacgaga tctttcacga tcgggaaaga gcgggcgcga
     2881 aacggctcga tcgtgatcgt cgcgccgtcc ttgaactcgc gcaggtagag ttggcaggcg
     2941 gcggccggat gcgacgggcc gtgggcttcg ccgtcgatcg ttaggctaca ggttccgcaa
     3001 atgccttcgc gacaatcgga gtcaaacgcg attggctctt cgccgcgggt cgtgagttgc
     3061 tcgttgagga tgtcgagcat ctcgaggaac gaggtggcgg gcgagatgtc gctgatctcg
     3121 tagggcttga gtttgccctt ggaatcggcc gcggtctggc gccagatctt gagtttgaaa
     3181 ttcatttcct tagggaaaaa ccgagccgcg aatgccgtca tcccgagcgg agccgaggga
     3241 tcccgcggcg ttaccggaga ggcaaatttc acgggatcct tcgactccgc tgcgctccgc
     3301 tcgggatgac gtgctcattt gtagctccgc gtcgcgagat gaaccgattc gaagacgagc
     3361 ggttcggtat gcaagttcgg ctcggtcccg tcgccctgat attcccaggc gaagacgtcc
     3421 gcgaaatttt cgtcgtcgcg ca
//
LOCUS       DATMNS010000063         5091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_83748, whole genome shotgun sequence.
ACCESSION   DATMNS010000063 DATMNS010000000
VERSION     DATMNS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5091
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83748"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="VNW28_02440"
     CDS             <1..285
                     /locus_tag="VNW28_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXA08810.1"
                     /translation="DDRHTVLYIGVTNELERRTNEHSLGRGSAFAKEYNAHKLIYFEA
                     YPDPQSAIAREKQLKNWSRGKKEALVAQANPAWRDLIDEMHAVRPKEGAR"
     gene            285..1217
                     /locus_tag="VNW28_02445"
     CDS             285..1217
                     /locus_tag="VNW28_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXA08811.1"
                     /translation="MPRDPSTPLRAARDDARTPAEILKKIRALEIKTKGLVQSAFAGD
                     YHSVFKGRGMNFEDVREYQPGDEIRAIDWNVTARLGNAFIKKFTEERELTVMLVVDVS
                     ASGNFGSVAQSKRELAAEVACLLAFSAIRNNDKVGLVLFSDQVELFIPPKKGRSHTLR
                     LIREILFFEPQGRGTLPSLALDYLNHVVTRRSVVFFISDFQAEDFSRALTITSRRHDF
                     IAIRIDDQREESLPDIGLITLEDAETGEQIEINTGDRRTRVRFSEAAQERRAEVTHTL
                     RRNNIDAISLRTGDDYLPALRSFFKQRERRMARR"
     gene            1228..1731
                     /locus_tag="VNW28_02450"
     CDS             1228..1731
                     /locus_tag="VNW28_02450"
                     /inference="COORDINATES: protein motif:HMM:NF025674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4381 family protein"
                     /protein_id="HXA08812.1"
                     /translation="MQIFLAQEFHDITGPIDYFLLQPWMIFCAVAGTLLLAGLTIWLI
                     KRWRNRPAAVVTPRQRALDLLARIETEIDLLTPYQFSIRVSDILRRYVTEQYQLPVTR
                     QTSVEFLNALAATSPFTGDEQSLLGDFLNRCDLIKFARYDATREDSRLLLDEAASFVR
                     GGALEPA"
     gene            1718..2782
                     /locus_tag="VNW28_02455"
     CDS             1718..2782
                     /locus_tag="VNW28_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXA08813.1"
                     /translation="MSLPDFWSPNSPWGFSFAHPWLLLLLLLVPLLAWLRGQRGAAAA
                     LVYSSTSTLRALGKKSTSRAGRIMHGLLYLALAIFIFALARPQLGKSLTTVEASGIDI
                     MVVLDVSGSMLTKDFTVGGDEATRVDAIREVTRKFIASRPNDRIGIIAFAGRPYVVSP
                     LTLDHDWLLQNLDRVRIGLVEDGTAIGSAMAAAANRLTDKKSKSRAIVLLTDGENNAG
                     KIPPNTAAEAIKALKIHFFAIGAGSNGIAPVPVFDQTNGRPITDVAGNIVYQNQPVEF
                     NEAGLKEVAKIAGGQFFRASDTDSLENTYRDIDKMEKSTVSVKKYQQYRDLFPVCLMA
                     GAGILLAELLLSQTIWKKLP"
     gene            2907..4754
                     /locus_tag="VNW28_02460"
     CDS             2907..4754
                     /locus_tag="VNW28_02460"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXA08814.1"
                     /translation="MTFGNPFWLWGLLVLPALAFFFARAEQRGADRLRQFVAARLLPQ
                     LAGTVNRGRRVFRFILLLLVLALVLASLARPRWGYAFDEVKRKGLDLILAVDVSRSML
                     SNDVQPNRLERVKLATQDLINELQGDRVGLIAFAGRAFLQAPLTIDYDAVVDSINDLD
                     TKTIPEGGTNISDAIALATRTYGKSATGNRALIIFTDGEDLSGDAVRAAKEAAEAGVR
                     IFTVGVGTASGSLIPIQSDDGGTAFVKDASGEVVKSKLDETRLREIARAADGFYLHLE
                     NGPQTMKQLFTEGLGKMQVADINARLSRRPIERYEWPLAAAILLFAIALLINDRQRAR
                     ARAATGGLGAVASSNQNHRRHRARPSGALAAAIVLLLVRQSHATSPGLQLYDQQKFPE
                     AYDHFEKTLQENPGAGQSDRIQFDAGAAAYKMQDYNKALQSFSQALLSPNPHLQSEAN
                     YNLGNTLYERGAAEKTDERKLTNWESALQHYTATLKAEPQNKNAKDNYDYVKKKIEEL
                     KKKQEEQQQQKQPPQPQKPPPPPQQSQSQQNQENSEGSAAKPTPPIQPSDAAKKAKAE
                     ADKAVLARQYLKALRIMTDLLRVDQTVNFYADYIHRLEEINGIKKTDNP"
     gene            complement(4762..>5091)
                     /locus_tag="VNW28_02465"
     CDS             complement(4762..>5091)
                     /locus_tag="VNW28_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08815.1"
                     /translation="GLTGRLPILRLLIFAGALAILRLRLLLLIFLLLVVLLLLIVLLL
                     LVVWVWRFLLVVEERQHGPKLRIIGEFSQTLLDLGSGKVFVAKDVFLRAPIYVVRGGL
                     RDKRERE"
BASE COUNT         1115 a         1575 c         1479 g          922 t
ORIGIN      
        1 gatgaccgcc acaccgtgct ctacataggc gtgacgaacg aactggagcg gcggacgaat
       61 gaacattcgt taggtcgagg aagcgccttc gccaaagaat acaacgctca taagctgatt
      121 tacttcgaag cttatccaga cccgcaaagc gcaatcgcgc gcgagaagca gctcaagaac
      181 tggagccggg ggaagaaaga agcgctggtc gcgcaggcga atccggcatg gcgggatctc
      241 atcgatgaga tgcatgcggt cagaccgaag gagggggctc ggtgatgccg cgggatccct
      301 cgactccgct ccgcgccgct cgggatgacg cgcgtacgcc agccgaaatc ctgaagaaga
      361 tccgcgcgct cgagattaaa acgaagggtc tcgtgcagtc ggctttcgcg ggcgattacc
      421 acagcgtctt caaaggacgc gggatgaatt tcgaggacgt ccgcgaatac cagccgggag
      481 atgaaattcg cgcgatcgat tggaacgtga ccgcccggct cgggaatgcg ttcatcaaaa
      541 aattcacgga ggaacgtgag ctgacagtca tgctggtggt cgacgtcagc gcctcgggaa
      601 atttcgggag tgtcgcgcaa tcgaagcgcg aactggcggc ggaagtcgcc tgcctgctcg
      661 ccttcagcgc gatccggaac aacgacaaag tcgggctcgt cctcttttcc gatcaggtcg
      721 agcttttcat cccgccaaag aaaggacgct cccacaccct ccgcctgatc cgcgaaattc
      781 tgttcttcga gccgcaaggt cgcggcacgc tgccgagcct cgcgctcgat tacctgaacc
      841 acgtcgtcac gcggcgctcg gtcgttttct tcatctccga ttttcaggcg gaggattttt
      901 cgcgcgcgct cacaatcacc agtcgacgcc acgatttcat tgcaatccgg attgacgacc
      961 agcgcgaaga gtccctcccc gacattggcc tcatcacttt ggaagatgcc gagacgggcg
     1021 aacaaatcga aatcaacacc ggcgatcgcc gcacccgggt gcgcttcagc gaagccgccc
     1081 aggagaggcg cgccgaggtc acccacaccc tgcggcgcaa taacatcgac gccatctcgc
     1141 tccgcaccgg cgacgattat ttgccggccc tgcggtcctt tttcaaacag cgcgaacggc
     1201 ggatggcgcg tcgttaggca gaagtcgatg caaatttttc tcgcgcaaga gtttcacgac
     1261 atcaccgggc cgatcgacta tttcctcctc cagccctgga tgattttctg cgcggtcgcc
     1321 ggcacccttt tgctcgctgg cctaacgatt tggctgatca agcgctggcg caatcgcccg
     1381 gcagccgttg tgactccacg gcagcgcgcg ctcgatctcc tcgcgcgcat cgaaaccgag
     1441 atcgacctgc tgacgcccta tcaattcagt atccgcgtct cggatattct gcgccgctac
     1501 gtcaccgagc aatatcaatt gcccgtcacc cggcagactt cggtcgaatt tctcaacgcg
     1561 ctggccgcga cctccccttt caccggcgat gagcaatcgc ttctcggcga tttcctgaat
     1621 cgttgtgatc tgatcaagtt cgcgcgctac gacgcaaccc gggaagacag ccggttgctg
     1681 ctcgatgaag cggccagttt cgtgagagga ggcgcgcttg agcctgcctg atttctggtc
     1741 gcccaattcg ccctggggat tttccttcgc gcatccctgg ctgctcctgc tcttgctcct
     1801 cgtgccgctg ctcgcctggt tgcgcggtca gcgcggcgcc gccgcggcgc tggtctattc
     1861 ttccacctcc acgctgcgcg cgctcggcaa gaaaagcacc tcgcgcgcgg gccgcatcat
     1921 gcatggcctg ctttatctcg ccctcgccat tttcattttc gcgctcgccc gcccgcaact
     1981 cggcaaaagc ctcaccacgg tcgaggcgag cggaatcgac attatggtcg tgctcgacgt
     2041 ttcgggttca atgttgacca aggatttcac ggtcggcggc gacgaggcaa cccgcgtcga
     2101 cgcgattcgg gaagtgacgc gcaagttcat cgccagccgg cctaacgatc ggatcgggat
     2161 tatcgccttt gccggccggc cctacgtcgt cagtccgctg accctcgatc acgactggct
     2221 tttgcaaaat cttgatcggg tgcggatcgg gctggtcgag gacggaacgg cgatcggttc
     2281 cgcgatggcg gcggcggcga atcggctgac cgacaaaaaa tcgaagagcc gcgcgatcgt
     2341 cctgctgacc gatggcgaga acaacgctgg aaaaattccg ccgaataccg cggcggaagc
     2401 gatcaaagcg ctgaagatcc atttttttgc gattggcgca ggcagcaatg gtatcgcgcc
     2461 ggtcccggtc ttcgatcaga cgaacggccg gcccattacc gacgtcgcgg gtaacatcgt
     2521 ttatcagaat cagccagtgg aattcaacga ggcgggcttg aaggaagtcg cgaaaatcgc
     2581 cggcggtcag tttttccggg cgtccgacac ggactcgctc gaaaacacct atcgcgacat
     2641 cgacaagatg gagaagtcga cggtctcggt aaaaaaatat caacagtacc gcgatctctt
     2701 cccggtctgt ctgatggcgg gcgcgggcat cctgctggcg gagttgctcc tgtcgcaaac
     2761 gatttggaaa aagctgccat gaggagctgg cctaacaata gcgccggaaa ggcgcgttcg
     2821 gagagacgtg ctttcgcacg tccaggctct ggtggcggca aggtaagaaa ctggacgtgc
     2881 cgccgcacgt ccctgcgggg aaaaccatga ccttcgggaa tccattctgg ctttggggct
     2941 tgctggtgct tccggcgctc gccttcttct ttgcgcgcgc cgaacagcgc ggcgcagacc
     3001 gactccggca gttcgtcgcg gcccgtttgc ttccgcaact cgccgggacg gtcaatcgcg
     3061 gccgacgcgt tttccgtttt atcctgctcc ttctcgtcct cgctctggtc ctcgcaagtc
     3121 tggcgcgacc gcgttggggc tacgcgttcg acgaagtgaa acgaaagggc ctcgacctga
     3181 tcctcgcggt cgacgtttcg cgcagcatgc tctccaacga cgtgcagccg aatcgcctcg
     3241 agcgggtcaa gctggccacc caggatttga tcaacgaact gcaaggcgat cgcgtcggcc
     3301 tgatcgcgtt tgccggccgc gcgttcctcc aggcgccgct cacaatcgac tacgacgccg
     3361 tggtggattc gatcaatgat ctcgatacca agaccattcc cgaaggcgga accaatattt
     3421 ccgacgcgat cgcgctggcc acgcggacct acggaaaatc cgcgaccggt aatcgcgcgc
     3481 tgattatttt caccgatggc gaggatctga gcggcgatgc ggtgcgcgcg gcgaaagagg
     3541 cggcggaggc gggcgtgcgc attttcaccg tcggggttgg gacggccagt ggctcgctta
     3601 tcccgatcca gagcgatgac ggtgggaccg cgttcgtgaa ggatgccagc ggtgaagtgg
     3661 taaaatcgaa gctcgatgag acccgcctgc gcgaaatcgc ccgggcggcg gatggttttt
     3721 atctccatct cgagaacggg ccgcaaacga tgaaacaact cttcaccgaa gggctcggca
     3781 agatgcaggt ggccgacatc aacgcgcgcc tgtcgcgccg gccgatcgaa cgctacgagt
     3841 ggccgctggc ggcggccatc ctgctcttcg cgatcgcact cttgatcaac gatcgccagc
     3901 gggcacgggc gagggcagcg actggggggt tgggcgccgt cgcgtcctcg aatcagaacc
     3961 accggcgcca ccgcgcccgt ccctccggcg cgctcgccgc ggcaattgtc cttttactcg
     4021 tcaggcaaag tcacgcgacg tcacccggcc tgcagcttta cgaccagcaa aaattccccg
     4081 aggcctacga ccatttcgaa aagacgctgc aggaaaatcc cggcgcggga cagagcgacc
     4141 ggattcagtt cgacgccggc gccgcggcct acaagatgca ggattacaac aaggcgttgc
     4201 agtccttcag ccaggcactg ctctcgccga atccgcacct gcaaagcgag gcaaactata
     4261 atctcggcaa cacgctttat gagcgcggcg ccgcggagaa aaccgacgaa aggaagctga
     4321 cgaattggga aagcgcgctc cagcattaca ccgcaacgct caaggcggag ccgcagaaca
     4381 agaacgccaa ggacaattac gattacgtca aaaagaagat cgaggagctg aagaaaaagc
     4441 aggaagagca acagcagcaa aaacagccac cccaaccgca aaaaccgccg ccgccgccgc
     4501 aacaatcgca atcgcagcaa aaccaggaaa actcagaagg atctgccgcg aaaccgactc
     4561 cccccatcca accgagtgac gccgcgaaaa aggcgaaggc cgaagccgat aaggcggtgc
     4621 tggcccgcca atatctaaag gctctcagga tcatgaccga tctgctccgc gtcgatcaga
     4681 cggtcaattt ttacgccgac tacattcacc gcctggagga gatcaatggc attaagaaaa
     4741 ctgataatcc ttagcctgtc tctactccct ctctcgcttg tcgcggagac cgcctcggac
     4801 tacataaatc ggggcgcgca gaaatacatc tttggccacg aagaccttgc ccgatccgag
     4861 gtcgaggagg gtttgagaaa attccccaat gatccggagc ttcgggccat gctggcgctc
     4921 ttccacgaca agaagaaacc gccaaaccca aacgacgagc agcaaaagaa caatcagcag
     4981 cagaagaacg acgagcagca aaaagatcag caacaacagt cgcaatcgaa gaatggccag
     5041 ggctccagca aagatcagga gccgcaaaat gggaagccgt ccggtcagtc c
//
LOCUS       DATMNS010000064         2767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4734422, whole genome shotgun sequence.
ACCESSION   DATMNS010000064 DATMNS010000000
VERSION     DATMNS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2767
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4734422"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1269
                     /locus_tag="VNW28_02470"
     CDS             115..1269
                     /locus_tag="VNW28_02470"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXA08816.1"
                     /translation="MNAGPIVAHARNVEAAVSAAQSSSARGLCESAGDTPASPLGTAR
                     LGAAERLLLCLLLLASALAPGARAANLDPDAVRRAAQYSAGHRGTSLLVIQNGETLLE
                     EYPGEGGANTPRRIYSGTKAFWDLAALAAAEDGLFALDDRVADTIAEWRDDPRKAQVT
                     IRQLLDFSSGLAPGFSLQENEYGDRDRDALRLPMVADPGTAFIYGPGALQVFHQLLKE
                     KLRKKSPARYLERRVLRRLGLGPQRYLRDHAGNPLLAAGFTLSARQWATIGHLVLARG
                     KPVVRPESLAQCWQGSAANRAFSLGWWNNEAAPGGREFDFEDMLTPKWSRQNWANACL
                     CRDAPSDLVACIGSGYQRLYVIPSLQLIVVRQGDGGRFSDGQFLRLLLGK"
     gene            complement(1367..>2767)
                     /locus_tag="VNW28_02475"
     CDS             complement(1367..>2767)
                     /locus_tag="VNW28_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08817.1"
                     /translation="LVGNFTVNAGGAAGIRWFELRGVTAGPVTVFQESTYQPDTTSRW
                     MGSAAMDGQGNLAIGFSASDGTINPQLRYAGRLVTDPLSSLAQGEAHLFDGTGSQTGS
                     GNRWGDYSALTVDPADDATFWYTNEYYSATSSFNWRTRIGSFKLPSVAPTPTPTPTAT
                     ATPTPTPTPTPTATPTPAPTSTPTPTPTPTPTPTPTPTPTPTPSTVLANISTRVVVGT
                     GANVGIGGFIVTGTQPKKVIVRAIGPSLSVTGNLANPTLALYGPDGVLIASNDDWRST
                     QEAEIIASTVPPADDLESAIVATLPASPTGMSYTAVMGGVNSTTGTGLVELYDLDRSA
                     DSKLANISTRGQVGTGDDVLIGGVIVVGSDPERVIVRAIGPSLGKVGLAGALQDPTLE
                     LYDGDGTLIASDDDWRETQETEIIATGIPPANNLESAIVATLPASPSGMGYTAIVRGV
                     NGTTGVALVEVYALAP"
BASE COUNT          506 a          861 c          900 g          500 t
ORIGIN      
        1 aaaaggagaa agccgcaaaa aaaagcgagc aggtaagaaa aaccatacca gcgaattccg
       61 aagctcggtc cgaacttgac gaggaacgga ctgaaattat cgagatagta cgccatgaat
      121 gccgggccaa tcgttgctca cgcgcgaaat gtagaggcgg ctgtgtcggc cgcacaatct
      181 tcttctgcgc gcggtctctg cgaatcggcg ggtgacacac ccgcctctcc acttgggacg
      241 gcgcggctcg gtgcggccga gaggctcctc ctctgtctcc tgcttctcgc ttcggcgctg
      301 gcgccgggtg cgcgggccgc aaatctcgac ccggacgcgg tgcgccgggc cgcgcagtat
      361 tcggccggcc atcgcggaac ttcacttctc gtcatccaaa atggggaaac gcttctcgag
      421 gaatatccgg gcgaaggggg cgcgaacacg ccgcgccgca tctatagcgg gaccaaggcc
      481 ttctgggatc tcgccgcgct cgccgccgcg gaggatggtc tcttcgcgct cgacgaccgg
      541 gttgcagata caatcgcgga gtggcgcgac gatccgcgca aggcgcaggt cacaattcgg
      601 cagctgctcg atttttccag cgggctcgcc ccgggattct cgctccagga aaacgaatac
      661 ggcgatcgcg accgcgatgc gcttcgctta ccgatggtcg cggacccggg gacagcgttc
      721 atctacggac caggcgcgct ccaggttttc catcaactcc tgaaggagaa actacgcaaa
      781 aaatcgcccg ccagatatct cgagcgccgc gtcctgcgcc ggctggggct cgggccgcag
      841 cgttacctga gggatcacgc gggcaatccc ctgctcgccg ccggattcac tctcagcgcg
      901 cggcagtggg ccacgatcgg ccacctcgtc ctggcgcgcg gcaaaccggt tgtccggccg
      961 gaatcgctcg cccagtgctg gcagggctcg gcggcgaacc gcgctttttc gttaggctgg
     1021 tggaacaacg aggcggcgcc cggcggccgg gagttcgact tcgaagacat gctgaccccg
     1081 aaatggtcgc ggcaaaattg ggcgaatgcc tgtctttgcc gtgacgcgcc gagcgatctc
     1141 gtcgcctgca tcggatcggg ctaccagcgg ctttacgtca tcccttcact ccaactcatt
     1201 gtcgttaggc aaggcgacgg cggccggttt tcggatggcc aattcctgcg tctcctcctg
     1261 ggaaagtgag gcagtcctgg agtaagggct tacggcgttt cgttaggcaa gaaaccgtca
     1321 tgctcggcgg cgcatcgccc gcagagaagg gacccggctg gctcgctcag ggcgcaagcg
     1381 cgtagacctc gaccagcgca acaccggtgg ttccgttcac gccacgcaca atggcggtgt
     1441 aacccatccc gctcgggctc gccggcagcg tcgcgacaat cgcggattcc aggttgttcg
     1501 ccggcggtat gccggtggca ataatctccg tctcctgggt ctcccgccaa tcgtcatccg
     1561 aagcgatgag ggtgccgtca ccatcgtaca gttccaaggt cggatcctgc agcgcaccgg
     1621 ccagacccac cttgcctaac gaaggtccga tggcgcgcac gatgactctc tccgggtcgc
     1681 tcccgaccac gatgacgccc ccgatcaaga catcgtcgcc cgtccccacc tggccgcggg
     1741 tcgagatatt ggcgagcttc gaatccgccg agcgatcgag atcatacaac tcgaccagcc
     1801 cggtgccggt agtgctattc acgccaccca taacagcggt gtaactcatg ccggtcgggc
     1861 tggccggcaa cgtcgcgaca atcgccgatt ccagatcgtc ggctggcggc accgtgctgg
     1921 caatgatctc tgcttcctgc gtgctccgcc aatcgtcgtt cgacgcgatc agcacaccat
     1981 ccgggccata aagcgcaagg gttggattcg ccaggttgcc cgtcaccgat agcgacggtc
     2041 cgatcgcccg cacgatgacc tttttcggct gtgtgccagt aacgataaaa ccgccaatgc
     2101 ccacattggc gccggtccca acaaccaccc gcgtcgagat gttagccagg acagtagagg
     2161 gagtcggagt cggggttggg gtgggagtcg gagtgggagt tggagttgga gttggggtgg
     2221 gagtcgaagt tggggcggga gtcggagttg cggtgggagt cggagtcggg gtgggagttg
     2281 gcgttgcagt cgcggtagga gtgggagtcg gagtgggagc caccgaaggc agcttgaagc
     2341 tcccaatcct ggtgcgccag ttgaagctgc tggtcgcgga atagtattcg tttgtatacc
     2401 agaaggtcgc gtcgtccgcc gggtcgacgg tcagggcgct gtagtcgccc cagcggttac
     2461 cggagcccgt ttggctgccc gtgccgtcga agagatgggc ttcgccctgc gccagggagc
     2521 tgagcggatc ggtcacgagc cggcccgcgt agcggagttg tgggttgatc gtgccgtcgg
     2581 aggcgctgaa accgatggcg aggttgccct ggccatccat cgccgcgctg cccatccagc
     2641 gcgaagtggt gtccggttga taggtgcttt cctggaagac tgtaaccggc ccggcagtca
     2701 cgccgcgaag ctcaaaccat cggatgcccg cagcgccacc cgcgttcacc gtgaagttgc
     2761 caaccaa
//
LOCUS       DATMNS010000065         2941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5091894, whole genome shotgun sequence.
ACCESSION   DATMNS010000065 DATMNS010000000
VERSION     DATMNS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5091894"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1946
                     /locus_tag="VNW28_02480"
     CDS             <1..1946
                     /locus_tag="VNW28_02480"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXA08818.1"
                     /translation="AEIEITRYTPKTLKAELGHTLPADECLRLGLLLAEALGALHRAG
                     LAHRDIKPSNIIFVQGAPKLADIGLVATSGQRSFVGTEGYVPPEGPGTPQADIYSLGK
                     VLYEISMGKDRLDFPELGSQLDERADKEQLLQLNEVLLKACAHQPARRYQNADALRND
                     LARIAAGQKSFRSSRLTIPLGIVAVLIVAAWAIHHFAAPPKTAPGPGTLFVTTDPAGA
                     MVVLGDRMERSPATFRGLAAGKYPLRLMMPDFDPVETELSIGPAQVLRPPLFKLVRSK
                     GSAQLASEPPGAEFELQRAGEPIRRGRTPATVRDLPTGRYNVVAKRGDWTLQDEIEIK
                     RGEVAFRTFQFANGSVKISSAPAGATVSSGGKDLGTTPLLLDEVKPGAVRYQLQLAGF
                     QPAEVQGSIRPNEQTFLAARLDRKRSLNRGAVWQNSLGMRFVPVSNWHISAWETRVRD
                     WAAFCNATRRGITRPDFAQSETDPVVEVDWNDATEFCQWLTEKERRANLLAENESYRL
                     PTDQEWSEAAGLPNEVGATPEERDGRVRDRFPWGSQWPPPRGAGNYGAIAGSGDRFPT
                     TAPVGSFPPNSLGIYDLGGNVWEWCLDPYKPGSRWGVLRGGSWANNQRSELLTSYRNV
                     VDRGERDVIYGFRCVLVSDTEQP"
     gene            complement(2073..>2941)
                     /locus_tag="VNW28_02485"
     CDS             complement(2073..>2941)
                     /locus_tag="VNW28_02485"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXA08819.1"
                     /translation="VRNWLCFPVTEKDGHYLVSRVDLAKTLEPELRPQMISNLGQFKT
                     VVLDPGHGGYDKGAMSPYGCEKDFALDVARNIKPLLEAKGLNVKMTRDSDVFVPLELR
                     ARIANVTKDSIFVSIHFNATDANPAANGFEIFSLTPRGAPSTADDALALRFMNMEAGS
                     PVDAPSLALSMSVYHSVVGHFSEFDRGIKRARFAVLRLTKIPAILVEGGFLTQRGESR
                     LIAQSTWRAKLAQSIATGIINYKGLVDNKTRPMMVADYRREFDGQLVLKDVSVPARLE
                     ASPAPVVIPASN"
BASE COUNT          541 a          902 c         1007 g          491 t
ORIGIN      
        1 gggccgagat cgagatcacg cgctacacgc caaagacgct gaaagcggaa ttgggccata
       61 ccctgccagc agacgaatgc ctgcgcctcg ggctcctgct ggccgaagcg ctgggcgcgc
      121 tccatcgcgc cggacttgcc catcgcgaca tcaaaccttc gaacatcatt ttcgtgcagg
      181 gcgcgccgaa gctggccgac atcgggctgg tcgcaaccag cgggcagcgc tcgtttgtcg
      241 ggacggaagg ctacgttccg ccggaggggc cgggcacgcc gcaggccgat atctacagcc
      301 tgggcaaggt gctctacgag atcagcatgg gcaaagaccg gctcgatttt cccgagctcg
      361 gcagtcagct cgacgaacgc gcggacaaag aacagttgtt gcagttgaac gaagtgctgc
      421 tgaaagcctg cgcgcatcag cccgcgcgcc gctaccagaa cgcggacgcg ctgcgcaacg
      481 acctggcgcg aatcgctgcc gggcaaaaga gctttcgctc ctcccgccta acgattccgc
      541 tggggatcgt cgcggtactg atcgtcgccg cctgggcgat ccatcatttt gcggctcccc
      601 cgaaaacggc tcccgggccg gggacactgt ttgtgacgac cgacccggcg ggcgcgatgg
      661 tcgtgctggg cgaccggatg gaacggagcc cggcaacctt tcgcgggctc gccgcgggca
      721 agtatccgct gcgtctcatg atgccggatt tcgacccggt cgagacggag ttgtcgatcg
      781 gaccggcgca agtgttgcgg ccacccctct tcaaactggt gcggagcaaa ggctccgcgc
      841 agctcgcctc cgagccgccc ggcgcggagt ttgagttgca gcgggcggga gagccgatcc
      901 ggcgcgggcg gacaccggcc accgtgcgcg acctgccgac cggccgctac aacgtcgtcg
      961 cgaaacgggg cgactggaca ttgcaggacg agatcgagat caagcggggc gaagtggcat
     1021 tcaggacctt tcagttcgcc aacggcagcg tgaagatcag cagcgcgccg gccggggcga
     1081 cggtttcgtc aggcggaaag gacctcggca cgacgccgct cctgctcgat gaggtgaagc
     1141 cgggcgcagt gcgctatcaa ttgcaactgg cgggtttcca accagcggaa gtgcagggca
     1201 gcatccgccc taacgagcag acctttctgg cggctcgcct tgaccggaag cgcagcctga
     1261 accgcggtgc ggtctggcag aattccctcg ggatgcgctt tgtgccggtg agcaactggc
     1321 acatcagcgc ctgggagacg cgggtgcggg attgggcggc gttctgcaac gcgacccggc
     1381 gggggatcac gcggccggat ttcgcgcaga gtgaaacgga tccggtggtg gaggtggatt
     1441 ggaacgacgc gaccgagttt tgccaatggc tgacggaaaa ggagcggcgc gcgaatctgc
     1501 tcgcggagaa tgaaagctat cgcctgccga ccgaccagga gtggagcgag gcggccggtt
     1561 tgcctaacga ggtgggcgcg accccggaag aacgggacgg aagagtgcgc gaccgattcc
     1621 cctggggcag ccagtggccc ccaccgcgcg gcgcgggcaa ctatggcgcg atcgcgggct
     1681 cgggcgaccg attccccacc accgcgcccg tcggcagctt cccgccgaac tcgttaggca
     1741 tctacgatct ggggggcaat gtctgggagt ggtgcctcga tccttacaag ccgggcagtc
     1801 ggtggggcgt gctgcgcggc gggtcgtggg cgaacaacca acgaagcgag ttgctcacgt
     1861 cttaccgcaa cgtggtcgat cgcggcgagc gcgatgtcat ttacgggttt cgttgcgtgc
     1921 ttgtttccga cacggagcaa ccgtgagccg agcgatcccc tcggagcgac gtgcttccgc
     1981 acgtcctgga caggcagaag cgtatccctc cgaatatgaa actggacgtg cggaagcacg
     2041 tccctccggg cggtcggcga aacgaacagc ggttagttgc ttgccgggat gacgaccggc
     2101 gcaggcgacg cttccaggcg ggccggcacg ctcacgtcct tcaggacaag ctgcccgtcg
     2161 aattcgcgcc gataatccgc gaccatcatc gggcgggtct tgttatcgac gaggcccttg
     2221 taattgatga tcccggtggc gatcgattgc gcgagcttcg cgcgccaggt gctctgcgcg
     2281 atcagccggc tttcgccacg ctgggtgagg aagccgccct cgacgaggat cgcggggatt
     2341 ttcgtcaggc gcaggacggc gaaacgcgcg cgcttgatcc cgcggtcaaa ctcggagaaa
     2401 tggccgacga ccgaatgata gacgctcatc gagagcgcga gactcggcgc atcgaccggg
     2461 ctgccggcct ccatgttcat aaaccgtagc gcgagcgcgt cgtccgcagt agacggcgcg
     2521 ccgcgcggtg tgagtgagaa aatctcgaaa ccgttcgccg ccgggttcgc gtcggtggcg
     2581 ttgaaatgga tgctgacgaa gatggaatcc ttggtcacgt tggcgatccg cgcgcgcagt
     2641 tcgaggggca cgaagacgtc gctgtcgcgc gtcattttta cattcagccc cttggcctcg
     2701 aggagcggtt tgatgttacg cgcgacatcg agcgcgaaat ctttctcgca gccgtaaggg
     2761 ctcatcgcgc ccttgtcgta gccgccgtgg ccgggatcga gcacgacggt tttaaattgc
     2821 ccgaggttcg aaatcatctg cggacgcagt tccggttcaa gggtcttggc cagatcgacc
     2881 cgcgagacga ggtaatgccc atccttctcg gtgaccggga aacagagcca gttgcggacc
     2941 c
//
LOCUS       DATMNS010000066         5757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_442501, whole genome shotgun sequence.
ACCESSION   DATMNS010000066 DATMNS010000000
VERSION     DATMNS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5757
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442501"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VNW28_02490"
     CDS             <1..969
                     /locus_tag="VNW28_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08820.1"
                     /translation="PVATSGTFSGKLELDFGTGAAATLAAAPGTLDLSIQQIDFQLNP
                     TAATIEPPSQPLNISTRLNVGTGDNVLIGGFIITGTDAKLVVLRAIGPSLMVSGVPGV
                     LADPFLELHDSTGAIVATNDNWMDNSTADQMVLTENNLAPTNNLESALVETLNPGEYT
                     AIVRGVGDTTGVALVEAYDLDNGTTNSVLGNISTRGSVETANNVMIGGFILGGGGGGF
                     STVILRGIGPSLASTGVPNVLANPFLELHDSNGDVIDSNDNWMDNPNMQQISDAGLAP
                     SDPNESALYEILPAGEYTAILSGVGATTGVGLVETYNIDNSPVSGR"
     gene            1098..1571
                     /locus_tag="VNW28_02495"
     CDS             1098..1571
                     /locus_tag="VNW28_02495"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HXA08821.1"
                     /translation="MPTLALRITTSVLLGWCALSTSAGASDEALARVVGNWLTEPHDG
                     IIQLTVENDGLLEGRIVGGNHPGLKDEKNPDPTRRGLDLRGQAILKDMHYDGDGHWSG
                     GTIYKPDNGRTYKCKVELVGDNALKVRGYLGFALIGISQKWTRYTGTSMILPPAR"
     gene            1922..3268
                     /locus_tag="VNW28_02500"
     CDS             1922..3268
                     /locus_tag="VNW28_02500"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXA08822.1"
                     /translation="MSRILIADDQPDVLEALRILLKGEGYQTDTVTSLGAMLNSLEKR
                     NYALLMMDLNYTRDTTSGQEGLEVIPRIQASDETLPIVVMTAWATIDLAVEAMKLGAR
                     DFVPKPWDNDRLLAIVRTQIALGSALRKSQQLEAKNELLRGKMPDIISNSPQMRPVIE
                     LITRVAPSDANVLITGENGTGKGLVARALHSLSKRASKIMITVNMGGLSEGVFESELF
                     GHVKGAFTDAKSDRAGRFELADESTLFLDEIANLPMSQQAKLLRVIETGEFERVGSSR
                     TLHANVRIISATNSNLKEEVAAGRFRQDLLFRLNTIEVALPPLRERREDIAPLAAHFL
                     RQHAERYRKKLTGFEPAAHEALIRHEYPGNVRELDHVIERAVLMAQGAQIKPNDLGLT
                     SATNGSPRLEEMSLEEVEAFLIKKALARHGGNARKAAESLGLSRSAFYRRLQHYGL"
     gene            3386..4729
                     /locus_tag="VNW28_02505"
     CDS             3386..4729
                     /locus_tag="VNW28_02505"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXA08823.1"
                     /translation="MDKPAHPLRRHHRLSHETRLTWLTLGAGAPAMIVALALLWFGDF
                     SAKVDWTLTLIIAVCAFGFLASARERIVRPLQTMSNLLAALREGDYSIRARGARADDA
                     LGEVLLEINQLGETLRVQRLGAFEATALLRTIMAEIDVAVFTFDPERRLRLVNRAGEN
                     LLRQPIDKLLGRRASDLGLAVCLDADEDAPLTLNFPGASGRWGVRRSNYREHGLPHEL
                     LVLTDLSRTLREEERKAWQRLVRVLGHEMNNSLAPIKSIAGSLETLLRRDPPPADWRD
                     DARSGLNVIAARAESLSRFMQAYARLARLPPPQKEEVDLGELVRRVIDLETRLAVRIA
                     EGPAIVIAADAAQIEQLLINILHNAVDAALETGGQVTAGWRELADYAEVFVRDEGPGI
                     MNPENVFVPFFTTKPGGSGIGLALSRQIAEAHGGTLTLANRRDRSGCEALLRLPR"
     gene            5083..5400
                     /locus_tag="VNW28_02510"
     CDS             5083..5400
                     /locus_tag="VNW28_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08824.1"
                     /translation="MKTTAQIQDLNQRHASDPHASRRGRSFPQTDHSYQSSTLLGGCG
                     KRVEFHGPSFLEISNRYFADEAPRSFAVEAGVFAALILTAVLPIVNSVQAVATLIHSV
                     GVL"
     gene            5419..>5757
                     /locus_tag="VNW28_02515"
     CDS             5419..>5757
                     /locus_tag="VNW28_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08825.1"
                     /translation="MQRSFEVIAGAQPAATPGFTPVTSSMDVPRPDVARRKKKRRLII
                     AAASVLGLIVITVLVSRLKPAAMSVDRASVWIDTVKRGPMLRQVRGLGTLVPEDIRWI
                     AAAKDGRVEKI"
BASE COUNT         1160 a         1831 c         1743 g         1023 t
ORIGIN      
        1 cccgtcgcta cgagcggtac cttctccggc aagctggaac tggacttcgg taccggagcc
       61 gcggcaacat tggccgccgc tccgggcact ttggatctga gcatccagca gatcgatttc
      121 cagctcaatc cgacggccgc aacgatcgag cctccctcgc agcccttgaa tatctccacc
      181 cgtctgaatg tcgggacggg tgacaatgtc ctgatcggcg gcttcatcat taccggcacg
      241 gatgcaaagc tggtcgtcct gcgtgcgatc ggcccgtccc tgatggtgtc gggcgtcccc
      301 ggcgtgctcg ccgacccctt cctcgagttg cacgattcca ccggagcgat cgtggcgaca
      361 aacgacaact ggatggataa cagcacggcc gaccagatgg tccttaccga aaataacctg
      421 gcaccgacta ataacctcga gtccgctctc gtcgaaaccc tcaatccggg cgaatatacg
      481 gccatcgtgc gcggagtggg cgatactacc ggcgtagcct tggttgaagc ctacgatctc
      541 gacaacggca cgaccaattc cgtgctcggc aacatcagca cccgcggctc tgtcgaaacc
      601 gccaacaacg tcatgatcgg cggctttatc ctcggcggcg gtggcggcgg tttctccacg
      661 gtgattctgc gcggcatcgg accttccctg gccagtaccg gagtgcccaa tgtgctggcc
      721 aacccgttcc tcgaactgca cgacagcaat ggcgacgtga tcgattccaa tgacaactgg
      781 atggataacc ccaacatgca gcagatcagc gatgccggtc tcgcgccctc cgacccaaac
      841 gagtcagcgc tctacgagat cctgccggct ggtgaatata ccgcgatctt gagcggggtc
      901 ggagctacca cgggggtcgg cctggtcgag acctacaaca tcgacaacag ccccgttagc
      961 ggccgataag gcagcctgac gcccggtcgg ggttttccag accgggcgtt ttctttttca
     1021 cagattggtt tctgagcgag actaacgttg agtggcaagc ggcaaggact ccgcctaaga
     1081 tcccacggga aaaaatcatg cccacactcg ctctgcgtat taccacttcg gtattactcg
     1141 gttggtgcgc cctcagcaca agcgcgggcg ccagcgatga agcgctcgcg cgcgtggtcg
     1201 gcaactggct gaccgaaccg cacgatggca tcatccagct taccgtggaa aacgacggtc
     1261 ttctcgaggg gcggatcgtc ggcggcaacc atccgggact caaggacgag aaaaatcctg
     1321 atccgacccg gcgcgggttg gacctgcggg gtcaggccat cctcaaggac atgcactatg
     1381 acggcgacgg ccactggtcg ggaggcacga tctacaagcc cgataatggc cgtacctaca
     1441 agtgcaaggt tgagttggtg ggggacaatg cgctcaaggt gcgcggctat cttggcttcg
     1501 cgctcatcgg catcagccag aagtggaccc gttataccgg cacatcgatg attctaccgc
     1561 cggcgcgcta gccgatttta cttcgccacg aaaggccaaa agatggggat gaggtgccaa
     1621 tcgtcccgct cacgagggga atcgcgggcg tggtgagcgc gaccagaccg ggcggaagcc
     1681 attacggagg aacgcgacag aggcgccaat gacgagcgcg aagacgaggg caaatgaatc
     1741 gtgtgcaacg tcatagggct ggcggtagac aggacctttt tcggcgagcc agccgcgcag
     1801 cacaaccgcg aaatcgcgcg tctgagcggg gccgcgaccg gactgtccct tcgcctaacg
     1861 agccgggccg ctccgctcgg agaattgacg gcgcgcgcga aatgcggttc atcgttatgc
     1921 tgtgagccgc atccttatcg ccgacgacca gcccgatgtg ctcgaggccc tgcgcattct
     1981 cctcaagggc gaaggttatc agacggacac cgtgacatcg ttaggcgcga tgctcaattc
     2041 gctcgagaag cgcaactacg cgctcctcat gatggatttg aattacaccc gcgataccac
     2101 ctcgggacag gaagggctgg aggtcattcc gcgcatccag gcgagcgacg agacgctgcc
     2161 catcgtggtc atgacggcct gggcgacgat cgatctcgcg gtcgaagcga tgaagctcgg
     2221 ggcgcgcgat ttcgtcccca agccgtggga caacgatcgc ctcctcgcca tcgtccgcac
     2281 ccagattgcg ctcggcagtg cgctccgcaa aagccagcag ctcgaagcga aaaatgaact
     2341 gctccgcggg aaaatgccgg acatcatttc caactcgccc cagatgcggc cggtgatcga
     2401 attgatcacc cgcgtggcgc cttcggacgc caatgtcctg atcacgggcg aaaacgggac
     2461 cggcaaaggg ctcgtagcgc gggcgctcca ttcgctctcc aagcgggcga gcaagatcat
     2521 gattacggtg aacatgggcg gcctctccga aggagtgttt gagagcgaac ttttcggcca
     2581 cgtgaaggga gcgttcaccg acgcgaagtc ggatcgcgcg ggacgtttcg aactggccga
     2641 cgaaagcacc ctctttctcg atgagatcgc gaacctgccg atgagccagc aggcgaaatt
     2701 gctgcgggtg atcgagacgg gcgagttcga acgcgtcgga tcgtcgcgca ctttgcacgc
     2761 caatgtgcgg atcatttcgg ccacgaattc caacctgaag gaggaggtcg cggccggacg
     2821 cttccggcag gatttgctct ttcgcctgaa cacgatcgaa gttgcgctcc cgccattgcg
     2881 cgagcggcgg gaagatattg ctcccctcgc cgcccacttc ctgcgccagc acgccgaacg
     2941 ctaccggaaa aagctgacgg ggttcgagcc ggccgcccat gaagcgctca tccgacatga
     3001 atatccgggg aacgtgcgcg aactggatca cgtcatcgag cgcgccgtcc tgatggcgca
     3061 aggcgcgcag atcaagccaa acgatcttgg cctaacgagc gcaacgaacg gctccccgcg
     3121 actggaagag atgagcctgg aggaagtgga agcgttcctg atcaagaagg cgctcgcccg
     3181 gcacggaggc aatgcgcgca aggccgccga gtcgttaggc ctgagccgga gcgccttcta
     3241 ccggcgcctc cagcactacg ggctctgaaa tgacgaggcc cgaagcccaa aacccgcgtc
     3301 cgatgctttc gtcatttagt cattggtcat gtcttcgtca tccggaattc gtcattcgtc
     3361 attctccccc acccagataa ggaaagtgga caagccagcc catccgctgc gtcggcacca
     3421 ccgcctttcc catgaaacac gccttacctg gctgacgtta ggcgcgggcg cgcccgccat
     3481 gattgtggcg ctggcgctcc tctggttcgg cgacttcagc gccaaggtcg attggacgct
     3541 gaccctcatc atcgccgtct gtgcattcgg ttttctggcc agcgcgcggg aacgcatcgt
     3601 tcgtcccctg cagacgatga gtaacctgct cgccgcgttg cgcgagggcg actactcgat
     3661 tcgggcgcgc ggcgcgcggg ccgacgatgc actcggcgaa gtgctcctcg agatcaatca
     3721 gctcggcgaa accttgcgcg tgcagcgcct cggagcgttc gaggctacgg cgctgctccg
     3781 cacgatcatg gcggaaatcg atgtggccgt ttttactttc gatccggaac ggcggctccg
     3841 cctggtcaat cgcgccggcg aaaacctgct ccgccaaccg atcgacaagt tgctcggccg
     3901 ccgggcaagc gatctcggcc tggccgtctg cctcgacgcc gatgaagacg cgccgctgac
     3961 cctgaatttc ccgggggctt ccggccgctg gggcgtgcgg agaagcaact atcgggagca
     4021 cggtttgccg cacgagttgc tcgtcctgac cgacctcagc cggactctgc gcgaggagga
     4081 acgcaaggcc tggcagcgcc tcgtcagggt gctcggtcat gaaatgaaca actcgctcgc
     4141 gccgatcaag tcgatcgccg ggagcctcga gaccttgctc cggcgcgatc cgccgccggc
     4201 cgattggcgc gatgatgcac gcagcggttt gaacgtaatc gcggcccgcg cggaatcgct
     4261 cagccgcttc atgcaggcct acgcccgcct tgcccgcctt cctccgccac agaaggaaga
     4321 ggtcgacctc ggtgaactcg tcagacgagt gatcgacctg gagacgcggc tcgcggtgcg
     4381 gatcgccgaa ggcccggcga tcgtgatcgc ggccgatgcg gcgcagattg agcagttgct
     4441 catcaacatc ctgcacaacg cggtcgacgc tgcgctcgaa accggcgggc aagtgacggc
     4501 cggctggcgc gaactggccg attacgcgga ggttttcgtg cgggacgaag gcccgggcat
     4561 catgaatccg gaaaacgttt ttgtgccgtt tttcaccacc aaaccaggtg gctcgggcat
     4621 cggcctcgcg ctcagccggc aaatcgccga ggcgcatggc ggcacgctca cgctcgctaa
     4681 ccgccgcgat cgttccggct gcgaagcgct tctgcgcctt cctcgttagg gcctaacgaa
     4741 cgccggggag gacgctggtt caccttccct cctggcatcc tgagccgcgc agacggcgaa
     4801 ggaccccgcc gatagagtcc acgcgccttc gtcgggccgc gccgactctc agcctgacaa
     4861 gccggaaagc gcgtcccgca ttcgggacag accaatccca tcgctgaggc aaaagcggcg
     4921 cggttaaaaa atcgagctta acacaagtat ctgaggtgca attatttata aaattaaaat
     4981 ttgactggca cgggcattgc aataggagtc ggcgcctccc gaaataattc tgaatccaaa
     5041 cgcgagatcg aacgcatctc accagtaacc agcagaaaga atatgaaaac cacagctcaa
     5101 atccaagatc tcaaccagcg gcatgcctcc gacccgcacg cttcgcgccg cggccggagt
     5161 tttccgcaaa ccgaccatag ttatcagagc tcgacgttgc tcggcggctg cggaaagcgg
     5221 gtggaattcc acggcccttc cttcctcgaa atcagcaacc gctatttcgc cgatgaagca
     5281 ccgcgcagtt tcgcggtgga agccggcgtc ttcgccgcgc tgatcctgac cgcggtcctg
     5341 ccgatcgtga acagcgtgca ggcggtcgcg accctcatcc acagcgtcgg cgtgctttaa
     5401 gcgcgccgcc aggtcatcat gcaaagaagc ttcgaagtga ttgccggcgc tcagcccgcg
     5461 gccactccgg gcttcacacc ggtcacttcg tccatggatg tcccgcgccc cgacgttgcc
     5521 cgcaggaaaa agaagcggcg gctaatcatc gccgccgctt cggtgctcgg ccttattgtc
     5581 atcaccgtgt tggtttcgcg cttgaaaccg gcggcgatga gcgtcgatcg cgcttcggtc
     5641 tggatcgata cggtgaagcg cgggccaatg ctccgccagg tgcgcggtct cggcactctc
     5701 gtgcccgaag acattcgctg gatcgccgcg gccaaggacg gccgggtcga gaaaatc
//
LOCUS       DATMNS010000067         5827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_89695, whole genome shotgun sequence.
ACCESSION   DATMNS010000067 DATMNS010000000
VERSION     DATMNS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5827
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89695"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2550
                     /locus_tag="VNW28_02520"
     CDS             <1..2550
                     /locus_tag="VNW28_02520"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXA08826.1"
                     /translation="TAYSGAGQFDGANKSITLRPNGQISTAEGYRNLIIKQAANASPV
                     YLKDVARVIDGVENERLSRHFFVRGLHPPASVIVFAVSRQAGANAVAVARSVRALLPG
                     LQRELPGSIRLIPVFDRSASIVNSLHDVQATLAIAFALVIFVIFVFLGRASDTLIPMV
                     ALPMSFFITFVAMWWFGFSINNLTLMALTLAIGFLVDDAIVFLENVVRRAEAGESIYR
                     ATLNSAGEISFTILSMTLSLAAVFIPLAFMPGLLGRIFREFAITIIVAIVASGLVSLT
                     LTPLMCARILAERGPGHRKSWTERLFSRVFEPTVRFYSRSLDWFLDRGWLALPIILAC
                     VLGVWFFFKQLPFTLLPTGDSGTIRGFFIVQEGASPDQQRRLQEKLDPVLQANPAVDK
                     YFTVAGRSGLGAGVFTALFLKDASQRADIDTVAAQLRKACSEIPGIFPTLNPQPVLQI
                     NIGATGSSFGRYSYVLSGINPNEVYDAADKLGARLAEYDGFASPPRSDLFRNTPNLDI
                     NIERGRASLYGVSTSNLENLLRAAYSQNYVYLIKQADDQYQVIVEADDKARSGPEDFR
                     QIYVKPDNGTKLIPIPALTNSKETLGLQSVNHTNQFTSVTFGFDTRPGAALGDVTNYI
                     QKTAAEVLPPTVKGELQGEGLVLQQLFGALPFLILAAVFVMYVILGILYESYVHPVTV
                     LSTLFPAVVGGLMTLWLFQSTLSLYSVIGLFLLMGIVKKNGIMIVDFALHRLDEGYDR
                     RAAIHEASVERFRPIIMTTLAALMGALPIAFGFGADGSSRRPLGLVIVGGLIVSQLIT
                     LYITPVIYLALEWFQERVLDRVPFLRSAHTHHEADAATHGSELEPAPAGVR"
     gene            complement(2558..2965)
                     /locus_tag="VNW28_02525"
     CDS             complement(2558..2965)
                     /locus_tag="VNW28_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08827.1"
                     /translation="MRQISSAAFGPLDDAAEVARAGVPTVMMFVQSLRGISHNKIEDT
                     REEHRALAVRAFDRLVSKAMEWMASRQGSARDSRAVSGDSPLTRRQRARKTESARDAD
                     CVCVEGSKVRFGESPKPARGSRALPGVRRVAFR"
     gene            complement(2976..5660)
                     /locus_tag="VNW28_02530"
     CDS             complement(2976..5660)
                     /locus_tag="VNW28_02530"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08828.1"
                     /translation="MNAEQHRLEQNDHREERWHLWGPYLSERQWSTVREDYSANGDAW
                     NYFPHEHARSRAYRWGEDGIGGICDFKQRLCFAFAFWNERDPILKERLFGLTGPQGNH
                     GEDVKEIFFFEDNTPSHSYMRMTYRYPQAAFPYEELVRRNAERSRMEPEFELWDTGVL
                     HEDRYFDITIEYAKAAEDDILIRATVTNRGPEAAALHLLPMLWFRNTWSWSADQPRPN
                     LRVGSGHSEELAVIEASHDALGEYRLAGEGAGPLLFTENETNVQLLYGVPNPSPYVKD
                     AFHEAIVRRNGAAVNPQRMGTKAAAAYRFSLGPSEARTVRLRLQRIKEPAILVFGDFD
                     RLFAQRREEADEFYRALAPPSLSEEHCAIQRQALAGMLWTKQFYYYVVEQWLDGDPAQ
                     PAPPNERKTGRNAEWRHLYNERVMSMPDTWEYPWYAAWDLAFHCIPLALVDPHFAKAQ
                     LDLILREWYQHPNGQIPAYEWNFGDVNPPVIGWAAWRVYKIEQHATGKGDRAFLETIF
                     HKLLINFTWWVNRKDSGGRNIFEGGFLGLDNIGVFDRNASFPDGSRLEQSDGTSWMGV
                     FCLTMMRTALELARENPVYENIATKFFEHFLAIAAAMNNVGGQGIGLWDEADEFFYDV
                     LHTPGNRYLPLRVRSIVGLMPLLAVETIEPALLDAMPGFKERLEWYLTNRPDLAGLIS
                     RWSEPGLGERRLVALTRGHRMKCLLRRMLDPNEFLSDYGVRSVSKFHEKNPYVLEVRG
                     ERKVVNYEPADSQTGIFGGNSNWRGPVWFPINYLLIESLQKFHHYYGDDFQVECPTGS
                     GQFVTLDQIANELSNRLIKLWLRDEKGERPFTRASGDSLGSAADRDRYLFHEYFHGDT
                     GAGLGANHQTGWTALVAKLIQQQGTRGTIVQLDPFTDL"
BASE COUNT         1067 a         1867 c         1670 g         1223 t
ORIGIN      
        1 accgcgtact ccggcgcggg ccagttcgac ggcgcaaaca agtcgatcac ccttcggcct
       61 aacggccaga ttagcaccgc cgagggttat cgcaatctca tcatcaagca ggcggccaac
      121 gcctcgcccg tttatctcaa ggacgtggcc cgggtcatcg atggggtcga gaacgagcgc
      181 ttgtcgcgcc acttctttgt ccggggcctt cacccgccgg cgtcagtcat tgttttcgcg
      241 gtctcccggc aggccggcgc gaacgcggtc gccgtggcgc gttcggtgcg cgcgctcttg
      301 cccgggttgc agcgcgagct gcccggctcg atccgtttga tcccggtttt tgaccgctcg
      361 gcgtcgatcg tcaactccct gcacgacgtc caggcgacgc tcgcgattgc cttcgccctc
      421 gtcatctttg tcatctttgt ctttctcggc cgcgccagtg acacgctcat tccaatggtg
      481 gcgctgccga tgtcgttctt tatcactttc gtcgcgatgt ggtggtttgg tttctcgatc
      541 aacaacctca ccttgatggc gctgactctg gccatcggtt ttttggtcga tgacgcaatc
      601 gtctttctcg agaacgtcgt taggcgagcg gaagccggag aatcgattta ccgcgcgacc
      661 ctcaacagcg ccggcgaaat ttccttcacc atcctttcga tgaccctctc gctggcggcg
      721 gttttcatcc cgctcgcctt catgccggga ttgttaggcc gaatctttcg cgagttcgcc
      781 atcaccatca tcgtcgccat tgtcgcctcc ggtctcgtct ccttaactct cactcccttg
      841 atgtgtgcgc gcattctggc ggagcgcggt cccggccacc gcaagagttg gacggagcga
      901 ctttttagcc gggttttcga acccaccgtc agattctaca gccgctcgct cgactggttt
      961 ctcgaccgcg gttggctcgc gctgcccatc atcctcgcct gcgtcctcgg ggtttggttc
     1021 ttcttcaagc agcttccatt tactcttctg ccaaccggcg atagcgggac gatccgcggc
     1081 ttttttatcg tgcaggaagg cgcctcgccg gaccagcaac gccggctcca ggaaaaactc
     1141 gatccggttc tccaggccaa tcccgctgtc gataagtatt tcacggtcgc ggggcgctcc
     1201 ggcctcggcg cgggcgtctt caccgctctc tttttaaaag acgcaagcca gcgcgcggat
     1261 atcgacaccg tcgcggcgca gctccggaaa gcatgcagcg agattcccgg tattttcccg
     1321 acgctcaatc cacaaccggt actgcagatt aacatcggag cgaccggcag ctccttcggg
     1381 cgctacagct acgtcctgag cggcattaac ccgaacgaag tctatgacgc ggccgacaaa
     1441 ctcggagccc ggctcgccga atacgatggc tttgccagtc caccgcgctc cgatcttttc
     1501 cgcaatacgc ctaacctcga catcaacatc gaacgcggac gcgccagcct ttacggagtc
     1561 tccaccagca acctcgaaaa cctcctgcgt gccgcctatt cccagaatta cgtctacctg
     1621 atcaagcaag ccgacgatca atatcaggtg atcgtcgagg cagacgacaa agcgcgctcc
     1681 ggcccggagg atttccggca gatttacgtc aagcccgaca acggcaccaa gctgattccg
     1741 atcccggcgc tgaccaactc gaaggagacg ctcggcctcc agtcggtcaa tcacaccaat
     1801 caattcacca gcgtcacctt tggcttcgac acccggccgg gcgccgccct gggcgatgtc
     1861 accaattaca tccaaaaaac cgcggccgaa gttttgccac cgaccgtcaa aggcgagctg
     1921 caaggcgaag gtcttgtcct gcaacaactc ttcggcgccc tgccctttct catcctcgcc
     1981 gccgtcttcg tcatgtacgt gattctcggc attttgtatg agagctacgt ccaccctgtc
     2041 accgtgcttt ccacgctttt tcccgcggtc gtcggcggac tgatgacgct ttggcttttc
     2101 cagtcaaccc tttcgcttta ttcggtgatc ggccttttcc tcctcatggg tatcgtgaag
     2161 aagaacggca tcatgatcgt tgacttcgcg ctccatcggc tggacgaagg ctacgatcgc
     2221 cgtgccgcga ttcacgaagc gagcgtggaa cgtttccgcc cgattatcat gaccacgctc
     2281 gcagccttga tgggcgcgct gccgatcgcc ttcggcttcg gagcggacgg ctcctcgcgc
     2341 cgtccgctcg ggttggtaat cgtcggcgga ttgatcgtct cacaattgat cacgctctac
     2401 atcacgccgg tgatttatct ggcgctggaa tggttccagg aacgcgtgct cgatcgcgtg
     2461 cccttcttgc gcagcgcgca tacccaccat gaagctgatg ccgcaacgca cggctcggag
     2521 ttggaacccg cgcccgccgg agtgcgctaa ttttcgccta acgaaaagcg acgcggcgaa
     2581 ctccggggag cgcacgcgac ccgcgtgctg gttttggcga ctcgccaaaa cggactttgc
     2641 ttccctcgac acacacgcaa tccgcatcgc gcgctgactc ggtctttcgc gccctctggc
     2701 gacgagtgag cggcgagtcg ccgctcacgg cacgcgagtc acgtgcgctc ccctgacgag
     2761 acgccatcca ctccatcgcc ttcgacacga gccgatcaaa ggcccgcacc gcgagcgcgc
     2821 gatgttcctc tctggtgtcc tcgatcttgt tgtggctgat cccgcgcagg ctttgcacga
     2881 acatcataac cgtcggcacg ccggcgcgcg ccacttcggc tgcatcgtcc agtgggccga
     2941 aggcagccga tgagatttgc cgcatagctc cgggatcaca gatccgtgaa tggatcgagc
     3001 tgcacaatcg ttccgcgggt cccttgttgt tgaatcagct tcgcgaccag agccgtccaa
     3061 ccggtctgat gattcgcgcc caggcccgcg ccggtgtcgc cgtgaaaata ctcgtggaaa
     3121 agatagcggt ctcgatcggc cgcgctgcct aacgagtcgc cggacgcgcg ggtgaaaggt
     3181 cgttcccctt tctcgtcgcg caaccagagt ttgatcaggc ggttcgaaag ctcgttcgcg
     3241 atctggtcga gggtcacgaa ctgtccggag ccggtcgggc attcgacctg gaaatcatcg
     3301 ccatagtagt ggtgaaattt ctggagcgac tcgatcagga gatagttgat cgggaaccag
     3361 accgggccgc gccagttcga gttgccgccg aagatgccgg tctgcgaatc cgccggctcg
     3421 tagttgacca ccttgcgttc gccgcgcacc tccagaacat agggattctt ctcatgaaat
     3481 ttgctcactg agcgcacccc gtagtcgctc aggaattcgt taggatcgag catccgccga
     3541 aggagacact tcatgcggtg cccgcgggtc agcgcgacga ggcggcgttc tccgagcccg
     3601 ggctcggacc agcgcgaaat tagccccgcg agatcgggcc ggttggtcag gtaccactcg
     3661 agcctttcct tgaagccggg catggcgtcg agcagtgcgg gctcgatcgt ttccaccgcg
     3721 agcaagggca tgaggccgac tatggaacgc acgcgcagag gcaggtagcg gtttcccggg
     3781 gtgtgcagga cgtcgtaaaa aaactcatcc gcctcgtccc agagcccgat cccttgcccg
     3841 ccgacgttgt tcatcgccgc ggcaatcgcg agaaagtgct cgaaaaactt ggtcgcgatg
     3901 ttttcgtaaa ctgggttctc gcgcgccaac tcgagcgcgg tccgcatcat cgtgaggcag
     3961 aacacgccca tccagctcgt gccgtcgctc tgctcgagcc ggctgccatc gggaaaagag
     4021 gcgttccggt cgaagacgcc gatgttgtcg agtccaagga aaccgccttc gaaaatgttg
     4081 cgaccgccgg agtccttccg gttcacccac caggtaaaat tgatgagcag cttatggaag
     4141 atggtctcga gaaaagcgcg gtcgcccttc ccggtggcgt gctgctcgat cttgtagacc
     4201 cgccaggcag cccagccgat gaccggcgga ttgacgtcgc cgaagttcca ttcgtaggcc
     4261 gggatctggc cgttgggatg ctggtaccat tcgcgcagga tgagatcgag ctgcgccttg
     4321 gcaaaatgcg gatcgacgag ggcgagcggg atgcaatgaa aggccaggtc ccaggccgcg
     4381 taccagggat attcccaggt gtcgggcatc gacataaccc gctcattgta gaggtggcgc
     4441 cattccgcgt tgcgtcctgt cttacgctcg ttaggcggag ccggctgggc gggatcgccg
     4501 tccagccatt gctcgaccac gtagtaatag aattgcttcg tccacagcat cccggcgagc
     4561 gcctgccgct ggatggcgca atgctcttca ctcagggagg gaggagcgag cgcgcggtaa
     4621 aattcatccg cttcctcccg gcgctgcgca aagagacgat cgaagtcgcc gaacacgaga
     4681 atcgccggtt ccttgatccg ttgcagacgc agccgaaccg ttcgcgcctc ggagggccca
     4741 agtgagaacc ggtaggccgc cgcggccttc gttcccatgc gctgcggatt caccgccgcg
     4801 ccgttcctcc tcacgatcgc ctcgtgaaag gcatccttca cgtagggcga tgggttcggc
     4861 acgccgtaga gaagctgcac gttcgtttca ttttcggtga aaagcagcgg gccagctcct
     4921 tcgccagcca accggtattc gccgagcgcg tcgtggctgg cttcgatcac cgccagctcc
     4981 tcggaatgac cgctcccgac ccgcagattt ggccggggct ggtccgcgct ccagctccag
     5041 gtattgcgaa accaaagcat cggcaagagg tggagagcag ccgcttccgg cccgcgattg
     5101 gtaaccgtgg cccgaatcaa aatatcgtct tctgccgctt tggcgtactc gatcgtaatg
     5161 tcaaagtagc gatcctcatg cagcacgccg gtgtcccaga gttcgaactc cggctccatc
     5221 ctggaacgct cggcattgcg cctgaccagt tcctcatagg gaaatgccgc ctgcgggtaa
     5281 cgataggtca tccgcatgta ggagtggctc ggcgtattgt cctcgaaaaa gaaaatctcc
     5341 ttcacgtcct cgccatggtt cccctgcgga ccggtgaggc cgaagaggcg ttccttcaga
     5401 atcgggtcgc gctcattcca gaaagcgaaa gcgaaacaga gccgctgctt gaaatcgcag
     5461 ataccgccga tcccatcctc gccccagcga taggcgcggg accgggcatg ctcgtgcggg
     5521 aaataattcc aggcgtcgcc gttcgcgctg taatcctcgc gcaccgtgct ccactgccgc
     5581 tcactcaggt acggacccca gagatgccag cgctcttcac gatggtcgtt ctgttcgaga
     5641 cgatgttgtt cggcgttcat caagggacgg tgattcgtta ggcgggctcg ccttctgatt
     5701 caataatgaa gtcggcgcct ctgtcgatca aaaccgcgtc ccgccgttgc tccgcggtcc
     5761 ggttgcattt gttgttaagc tccgagcggg ttggatgtat ggttgcctcg ctggcacgtc
     5821 gaaaaaa
//
LOCUS       DATMNS010000068        18815 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_90225, whole genome shotgun sequence.
ACCESSION   DATMNS010000068 DATMNS010000000
VERSION     DATMNS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 18815)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18815)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18815
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90225"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..2303)
                     /gene="ftsH"
                     /locus_tag="VNW28_02535"
     CDS             complement(267..2303)
                     /gene="ftsH"
                     /locus_tag="VNW28_02535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXA08829.1"
                     /translation="MAQPERNSQKDNKRGSDPNFNWRGVILFAIAFALIGLALLFRGG
                     TGYGTEEDVPYNRFLELVEHKQIANDPKFPLRLVVEDGRPTQTIRGAYSRPGVGNAPP
                     QQVPFRTTVYLNFTPDLQEKLAAAHVPIAIRTESNVLAQAVIGFVPIALFLLILYFLF
                     RQQIRMAGKGALNFGKSKARMLARDKNKITFKDVAGVEEAKDEVQELVEFLRDPKKFQ
                     KLGGRIPKGVLLVGPPGTGKTLLARAIAGEADVPFFSISGSDFVEMFVGVGASRVRDM
                     FEQGKKSAPCIIFIDEIDAVGRHRGHGVGGGHDEREQTLNALLVEMDGFDTQEGVIII
                     AATNRPDVLDPALLRPGRFDRQITVNLPDVKGREEILRVHSKKVKLAEGVDLAVVARG
                     TPGYSGAELANVINEAALLAARRGLKGITLAELEESRDKVRWGKERRSLALSEKEKTN
                     TAYHEAGHALLLELLEFTEPLHKVTIIPRGPSLGSTMWLPLEDKYTQRRNELLDDLVV
                     SMGGRVAEEIVFGDVTSGARGDIQMATRLARKMVCEWGMSDKMGMVEYGEHEDYVFLG
                     RDISRARDYSEATAEQIDQEVRKLVDEAYARAKEILLANREKLEVIAKALLEYETLDG
                     VQIREVIEHGRLINPPPGPPPPSGSKLAPEKPPKQIVAPDVAPPLGGGLSGAPA"
     gene            2436..6047
                     /locus_tag="VNW28_02540"
     CDS             2436..6047
                     /locus_tag="VNW28_02540"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA08830.1"
                     /translation="MSESVSTFADKIDRAVQRVTPFAYRLRRRGRLWMRRALRKPRSV
                     AEGPNLLPLLIQVLASFSKADGVLLEEEIDSSLGFLRYDYPEAVYSELRMLFRQALYE
                     QQDLSAMAQKLAASLSADRKIMLGVQLYDLISQAGLKQEQVVAFYSFMSQLGMAAQAI
                     DIVYQLNASEDSDQAVYQRGASPLEAISFGGNGQSDVILKSLFGNERLLAFRYHDLIL
                     LKNYSTQAVSVRGRPLERGGFCRIYPGQRILIGDYVLSFQDLATYFNAKKNVSLPQIF
                     VRVNKDSDEVELERSRTRESSLEVTFGLTVRVKALRDVRATLNGVKLKANTQVEAALD
                     DRIVFHNDTEMELLDLRRRARAMGGRFQLKASKSEYLVSNNPSLLEEDDILLSPGTAG
                     DVLLKISCDYEKRIGVLEVLEADRPIMVGDVAVRTTAPLTDGDTIRIDVGQFLRCDFS
                     ERIIEEERNIIRSLEVIDVNHRFSNSDVALEGISFALNRGELVCVMGASGCGKSTLMR
                     VLGGQLQPSRGQILLNGQPLYDSLDTLKRYVSYIPQDDAFDEHLTIGENLQFAAAIRS
                     PHLSRRDRTRRLEAKLIELGLSERRDSIVGSPVKKILSGGERKRLNIGLDMIGSADVY
                     LFDEPTSGLSSKDSEHVIEIIRGMAHNKIIVVTIHQPSSKIFQMFHKAILLDKGGRLV
                     FFGTPSDMLRYFAEAEHQHQFGADLGACAACGTTRPEFIFDVLETPLRDLSGDIIYEE
                     NNRGQLMPARRYSPEFWRDKYEAFRLIQDVKQVPLRRDPVLPLPPDPPQKARELFRWH
                     DEWTQLRTLLHRAFISKLRNRVNLVITIGVAPVLALLIATILRYSDSGRYDFASAYHI
                     PTFLFLTLIVVMFLGLTNSADDIIRDRAVLQRERNLNVRLTYYVFSKTVSLGVFALVQ
                     CVLFVLIGNYILEIRGMFWPDLAVVFMTAMSGVSLGLVVSSLVADPKTAANIVPLVLI
                     PQIIMGGALIKYEDMNRNLGLVYTFARWFHEHPDRDETQKLDSRLQVPFVCEFIAMRW
                     SYEELIVAQAKLNPLTRRQDEAQRQIDAIVARHEGQPGDAKRLDDLKETLAVLSGLEA
                     HTPRELNQYLALIDRILNGKTSFDRAAFQNANGPITAEQLFVNQKVSDLIANAEMEQN
                     DYRRGGKPNVFFGAQKRYYGATVTVFTFNTAVLIGSSLGLLLLLHWILRRQLEVRRK"
     gene            6220..7350
                     /locus_tag="VNW28_02545"
     CDS             6220..7350
                     /locus_tag="VNW28_02545"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXA08831.1"
                     /translation="MKKNPPKPRLIVAASENDPDMLYATRFFAPDAFIFLEQNGRRIL
                     VLSDLEIDRGRKQAAADEILPYSAFEKEVQGTSAKAPAYEKVVAHFLTKRKVRSAVVP
                     ANFPLGLAREIESKKISLQPTNGLFWPERETKAEPELRLLRKALEITEAGMARGFEVL
                     KRAQPGKAQKLQWSGAVLTSEILRAEIDTAVLRAGGLPANTIVAGGDQACDPHERGHG
                     PLKANSLIIIDIFPRSAATGYYGDLTRTVVRGRASDEQRRLWETVNAGQALALKKMKP
                     GVDGLKLHKEVKQFFTDRGYPTEVRDGRQTGFFHGTGHGLGLEIHEFPRFQKTVFKPG
                     QVLTVEPGLYYPGLGGTRTEDVVAITTRGIRMLSRCENRLEL"
     gene            7512..8213
                     /locus_tag="VNW28_02550"
     CDS             7512..8213
                     /locus_tag="VNW28_02550"
                     /inference="COORDINATES: protein motif:HMM:NF016206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HXA08832.1"
                     /translation="MYAPYYGGLFSSNYLILVGIPLVIALWAQFRVSGAFKKWGAVRA
                     SSNITGAEAAREILQAANIGDVNVVEINDYLGDHYDPTQKRLCLSSNVYNTPSVAALG
                     IAAHETGHAIQHAKAYAPLKARMAVVPVTGIASRILPFAIFGGLFFHITGLITIGIYC
                     YLALLVFQLITLPVEFDASRRAKIILQQMGIIQPGNEALGVNKVLNAAAWTYVAAFVA
                     TLGNLLWLLSVRDRR"
     gene            8303..8899
                     /locus_tag="VNW28_02555"
     CDS             8303..8899
                     /locus_tag="VNW28_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXA08833.1"
                     /translation="MDQPRTDEDQALVARAQSGDAAAFDDLVVKYSPRLYGLIYNMTS
                     NHEDTNDMLQDVFAKAYRAIKGFRGKSSFYTWIHTIAVNMTLNFLKKRGRRYHLSLDD
                     VDASIQNDKEFIELTSTSSPVREADLSELQRRLNEAMMKLSDEHRAVVTMFHIQGMPH
                     AEISKILGVSEGTVRSRLFYANRQLQNYLDEFRKNPVG"
     gene            8912..9430
                     /locus_tag="VNW28_02560"
     CDS             8912..9430
                     /locus_tag="VNW28_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08834.1"
                     /translation="MDNFDENEIGKLLRLKKYEQPPPGYFENFLHEFHRRQRDELLRQ
                     PVWSVLWQRLSDSLFRLNIRSLTSYPAAVAAVLVCAAVISLKVYQAPSSAGDAMVDTS
                     TLPSNESVIDQMPSQRVADYFTLSSSLPTRRLGPDLSRQMNESAYTHRATTPPRYVLD
                     RLPVSYEPSFNF"
     gene            9408..10472
                     /locus_tag="VNW28_02565"
     CDS             9408..10472
                     /locus_tag="VNW28_02565"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HXA08835.1"
                     /translation="MSRLSISSQASRSFLSRGCTAGLASLCALVSLSLPALRAQDDTA
                     GAISREVKSLFERCGQAIVKIHAVDEHSELSGTGFFVDPTGTIYTSYSVGGEASNFTV
                     QFNGKKYHATQLMADLRSGIALLKVDAATPFLPIGKSAELGLATPVVAIGYPLDLPES
                     PSFGMIAGFDRKYLGRYFATTHLRINLPTQRGEAGAPLLNFKGEVVGILVSSVDSGSA
                     CYALPIDAAEKIRRDYVRFGDARHGWIGIDVHATENGVGGSNAEMTEIHEGTPAAGSG
                     LKTGDILLQVGRVAVHEPEDVIDASFFISAGDSVPITVMRGKEKLTFNVQAASAKPEA
                     MALGPSKKSAVPLKLEATPR"
     gene            complement(10479..12482)
                     /gene="ligA"
                     /locus_tag="VNW28_02570"
     CDS             complement(10479..12482)
                     /gene="ligA"
                     /locus_tag="VNW28_02570"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXA08836.1"
                     /translation="MAETEASAAQRIAALRAEIEEHNRRYYEEAAPTISDREYDRLYR
                     ELVDLEERFPQLITPDSPTRWVGGEPLKAFAQITHRVPMLSLDNTYSEEEVGAFYRRL
                     EKLLPNEKIPVVVEPKVDGVAISLLYEDGRLKYAATRGDGTTGDDITQNIRTIRSVPK
                     QLKGDAPRLFEVRGEVFMTKKGFVKLNAERSEAGLPVFANPRNSAAGSLKQLDPAMTA
                     QRPLGVIFYGTGAVEGMELTHHSKLFPLLKKLGLPCSERWWPADSVEGILRAIRELEE
                     VRHDFVYETDGAVVKVDAFAQRELLGMTAKAPRWAMAFKYEAERVETKLLDILIQVGR
                     TGVLTPVAALEPVVVSGSRVARATLHNEEEVARKDIRIGDTVVIEKAGEVIPAVVSVR
                     TDLRTGSEKKFRIPKKCPVCGSQVVKDEGQVAVRCVNAQCPAQLKRRVEHFAARGAMD
                     IEGLGEAMVEQLVEHKLVREVSDIYRLTSGQLGELERMGEKSVANLCAAIERSKRQPL
                     WRLLFGLGILHVGVSAARALADHFPNLDALLAATPEDLQRIPDVGEVVGASISQFFRE
                     PGNREMIEKLRAAGLRLTSDRKAAPPSDSKIKGTTWVITGTLSKPRDEIAEEIVQHGG
                     KVSGSVSKKTSFVLAGEEAGSKLEKARQLGLRVVDEAAFRRML"
     gene            12571..14310
                     /locus_tag="VNW28_02575"
     CDS             12571..14310
                     /locus_tag="VNW28_02575"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HXA08837.1"
                     /translation="MERILGIEGGGTKTSWMLFDARGGELQLLDDGSLAPTNFSLTSR
                     ERIVQIWREMPRNVEAVGAFLAGCGTPTDRAELTKLCAEVWPGAAIVTGSDRESGFAS
                     AFGEGDGIAVNAGTGSSITGRREGRFERAGGWGHVLGDAGGGYDLSLEMLRFVLRDYD
                     LHRRGSELAQEVLRALGLNDLNELVRWAQTAGKMEIATLAPLVFAAGAAGHKEVNDIL
                     CAGARVLAEYTCAVADRLEFRAPPVALLGGLFRGHSIYVSAYRRELAELSPVANVLVA
                     EKPSALGAAWLALAQRPPLAPHLEKPAPEIAAAATEGINPRSLDLENLSPRELVDLFV
                     AEEKFVQEALRAAADRLAEGVSLVATALKSKGRLFYVGAGTSGRLGVLDASEIPPTFG
                     AAPELVQGIIAGGAAAFQRSAEGAEDDGHAGGLAIVERGVRANDVVCGITASGRTPFV
                     LGALRRARERGARTLLLTSNPAASSLPNEFDLCIVLPTGPELLAGSTRLKAGTATKVA
                     LNILSTGAMVQLGCVRGNLMVELSATNQKLRARAVRLLVQLTGCDAETARARLASHGW
                     NLRAALAGGDAAG"
     gene            complement(14307..14729)
                     /locus_tag="VNW28_02580"
     CDS             complement(14307..14729)
                     /locus_tag="VNW28_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08838.1"
                     /translation="MMLCKNFFRFAFCILLGGAVAQGSPFGSWSASGVVVNKTFRSTP
                     LTDSLGVDGIYKLELRDRDNKVRRQMVTRAVFLVYEIGDEFNELAWRHKTPKARITSQ
                     HAVAAEADNSSPVTKVAIKDRLANPHFTQDMLPENEGF"
     gene            14903..15052
                     /locus_tag="VNW28_02585"
     CDS             14903..15052
                     /locus_tag="VNW28_02585"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HXA08839.1"
                     /translation="MLWTIFVILLVLWLLGLVSSYTLGGFIHILLVLAIVVLIINLVS
                     GRRSI"
     gene            15056..15274
                     /locus_tag="VNW28_02590"
     CDS             15056..15274
                     /locus_tag="VNW28_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3185 domain-containing protein"
                     /protein_id="HXA08840.1"
                     /translation="MKPAGIIGLILIVVGIIALAYGGFSYTKREKIIDAGPLQVSADR
                     EHTVPLPPVLGALALAGGIVLVLAGNKK"
     gene            complement(15388..16767)
                     /gene="lpdA"
                     /locus_tag="VNW28_02595"
     CDS             complement(15388..16767)
                     /gene="lpdA"
                     /locus_tag="VNW28_02595"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXA08841.1"
                     /translation="MNYDLVVIGGGPAGYVGAIRAAQLGERVACVEKERAGGTCLNWG
                     CIPTKSLLRNAELYHLMKHRAADFGFKIDGLSFDWSKVIKRSRDVSDKNAAGIEFLFK
                     KNKVDYIRGEASMDKPGEVLVKLAEGKTETHAAAKILVATGVLSRALPGLPFDGKKVI
                     GSREAMILDPQPKSMVIIGAGAIGVEFAYFYNAFGTRVILIEMLPDVLPVEDTEVSQA
                     LEKALTKQGIKILTNTKTAKTETTKTGVRIAVEGKSAETIEADVCLVAIGVRPLLPNG
                     KLKLELTGRGYIQTDDRYETDAQGVFAAGDVIGPPWLAHVASWEAIQAVEGMFGEIEP
                     RKIREFPGCTYCQPQVASIGLTERAAQESGKKFRIGKFPFSASGKARAIGETEGFVKL
                     IIGEPHGEILGAHIIGPEATEMIAELGLAITLEATYEEIEATIHAHPTLSESVHEAVG
                     QAFGHAIHI"
     gene            complement(16802..>18815)
                     /locus_tag="VNW28_02600"
     CDS             complement(16802..>18815)
                     /locus_tag="VNW28_02600"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA08842.1"
                     /translation="FFDELKRRKVYRVAVAYVIAAGGIIQLASAVFPAWELPNWSLRL
                     VIVLLLVGFPIALILAWAFDITSTGIQTTPALPAAGDPTNPHRRRNVFLLGAVGLTVA
                     IVAGFFLLPHVAAKKVDKSIAVLPFENFSDDKSNAYFADGIQDDILTTLSRISDLKVI
                     SRTSVMGYRGSTRNVREIGKALGAGAILEGSVRSDGKRVRVNVQLINTENDEHLWANE
                     YDRELTDVFAIQTDLAHEIAGALQARLSPTEEARVSQKPTENGEAYLAFVEAHDLQMR
                     PDHLETDIQKAEELYERAIQLDPKFALAFAELSCLQSWIYHTSDPTSARQEKARVAAE
                     EAQRLQPNLPETHAALGYYYYWTQRDYERALGEFAAAQHGLPNDAENLKAIAAIQRRQ
                     GKWNESTANFEKAVSLNPKDADLLLNLAFNYIALRRYDEADRTVDRGLQAAPDSFSLR
                     GVKAQIALTGRGDFSVAESLLANAPDGFDPDGLISFVRANVWIMQRKFTAALQVLQNC
                     PGEILHSMGTAPVPKSFSEGMIYLYMHENEKARAAFQRARPMVERLIRESPDDASRHL
                     LLAQLLVGIGDKQGGIAEADRAAQLLPESRDAFDGPQVTIGQAQVLAWAGENERALEL
                     IKRSLSTPNGTTVPLLKIDPGWDPIRSDPRFQQLIATTGRSPDEHD"
BASE COUNT         3581 a         5951 c         5558 g         3725 t
ORIGIN      
        1 cgattcgggg actgcgcgac gccaaacgcc taacgttcaa cgttcaacga agacgcgggc
       61 gttgcccttg ccgccatcgt agctggctgt ggccgccgcc ctgtgggcgg cgcgggcatg
      121 ggtcaggtcg gcttgctaga ttccagcctc tctcccgcgc ttcgcacagc gaagcggcta
      181 catctatggc accaccatca gcgcaagtgc gctgccgtca ccgcctccgt agctggcttg
      241 gagattgctg ccgtttatgt tcgcgatcag gctggcgcgc cgcttaggcc gccaccgagt
      301 ggtggtgcca catccggcgc gacgatctgt ttgggcggct tctccggtgc gagcttcgaa
      361 ccggaaggcg gcggcggccc cggcggcgga ttgatgagtc gcccgtgctc gatcacttcg
      421 cgaatttgca caccatcgag ggtctcgtat tcgagcaagg ctttcgcgat gacttcgagc
      481 ttctcgcggt tggccagcaa aatttccttc gcccgggcat aagcctcatc gaccagcttg
      541 cgcacttcct ggtcgatctg ctcggcagtc gcttcgctgt aatcgcgcgc ccgggagatg
      601 tcgcggccga ggaagacata gtcttcgtgc tcgccgtact cgaccatccc catcttgtcg
      661 ctcatgcccc attcgcaaac catcttgcgc gcgagccgcg tcgccatctg aatatcgccc
      721 cgcgcgccac tggtcacgtc gccaaaaaca atctcctccg cgacccggcc acccatgctg
      781 acgacgagat cgtcgagcaa ctcgttgcgc cgctgggtat acttatcctc gagcgggagc
      841 cacatggtcg agcctaacga cggcccgcgc gggataatcg tgaccttgtg cagcggctcg
      901 gtaaattcca aaagctcgag cagaagcgcg tgcccggctt cgtgataggc ggtgttggtc
      961 ttttcctttt cgctcagcgc gaggctgcgt cgttctttgc cccagcgaac tttgtcccgg
     1021 ctttcttcaa gctcggcgag agtaattccc ttcagaccgc gacgcgcggc cagcaaggcc
     1081 gcttcgttga ttacgttggc cagctccgcg cccgagtaac cgggcgtgcc gcgcgcgaca
     1141 acggcgaggt cgacgccttc ggccagtttc actttcttgg aatggacgcg caggatttcc
     1201 tcgcggcctt tcacatcggg aagattgacc gtgatctggc gatcgaaacg ccccggacgc
     1261 aggagcgccg ggtcgagcac gtcgggccgg ttggtagctg caatgatgat gacgccttcc
     1321 tgcgtgtcga agccgtccat ctcgacgagt aacgcattaa gcgtctgctc gcgttcgtcg
     1381 tgaccgccgc cgacaccgtg tccacgatgg cggccgaccg cgtcgatttc gtcgatgaaa
     1441 ataatacaag gcgcggattt cttgccctgc tcgaacatgt cgcgcacccg gctcgcgccc
     1501 acgcccacga acatctcgac aaaatccgag ccgctgatgg agaagaacgg cacgtccgct
     1561 tcgccggcga tcgcccgcgc aaggagcgtc ttaccggtgc ccggaggacc gacgaggagc
     1621 acgcctttcg ggatgcggcc gccgagtttt tgaaacttct tcgggtcacg cagaaactcg
     1681 accaactcct ggacttcgtc cttcgcttcc tcgacgcccg caacgtcctt gaaagtgatc
     1741 ttgttcttgt cccgcgcgag catgcgcgcc ttcgatttgc caaagttgag cgcgcccttg
     1801 cccgccatcc ggatttgctg gcggaaaaga aaatagagaa tgagcaggaa aagggcgatc
     1861 ggaacgaagc cgataaccgc ctgcgcgaga acgttcgact cggtcctgat cgcgatcggc
     1921 acatgggcgg cggccaactt ctcctgcaaa tcgggagtaa aattcaaata aaccgtcgtc
     1981 cgaaatggca cctgctgggg cggggcattg ccgacccccg gccgggaata agcgccgcga
     2041 atggtctggg tcggccggcc atcctcgacc accaggcgga gcggaaattt cgggtcgttc
     2101 gcgatctgct tgtgctcgac caactcgaga aagcgattgt agggcacgtc ttcctcggtg
     2161 ccgtagccgg tcccgccacg gaagagcaag gccagaccga ttaaggcaaa ggcgatggcg
     2221 aagaggataa cgccgcgcca gttaaagttg gggtcgctgc ccctcttgtt gtctttttgg
     2281 gaatttcgtt cgggctgggc cataggagat gcagcttttt ggaaaggcgc gagcgcgaaa
     2341 ctatcatgaa gcctcggcca atgcaaaaga gcaggacccg ggccgccgca ttgcaccgat
     2401 ggccgcgtgg acgtaatctg ggcccggtaa atttcatgtc ggaatcggtt tccaccttcg
     2461 cagataagat cgatcgcgcg gtccagcgtg tgactccgtt cgcctaccgg ctgcggcggc
     2521 gcggccgact ttggatgcgg cgcgccctgc gcaaaccgcg gagcgtggcc gagggaccga
     2581 acctgctccc gctcctcatc caggtgctcg cgagtttctc caaagccgac ggggttctcc
     2641 tcgaagaaga gatcgactcc agcctgggct tcctgcgtta cgattatccg gaggccgttt
     2701 actccgagtt gcgcatgctc ttccggcagg cgctttacga acaacaggat ctctccgcga
     2761 tggcgcaaaa gctcgccgcc tccttgagcg ccgatcgcaa gatcatgctc ggcgtgcagc
     2821 tctacgatct gatttcgcag gccggcctca aacaggaaca ggtcgtcgcg ttttactctt
     2881 tcatgtcgca gctcgggatg gccgcgcaag cgatcgatat cgtctatcag ctcaacgcct
     2941 cggaggattc cgaccaggcg gtttatcagc gtggcgcctc gcccctcgag gccatttcct
     3001 tcggcggcaa cggccagtcc gacgtgatcc tgaagagtct cttcgggaac gagcggctgc
     3061 tcgcttttcg ctaccacgat ctgatcctgc tcaagaacta cagcacccag gcggtttccg
     3121 tccgcggccg gccgctcgag cgcggagggt tctgccggat ttatcccggc cagcgcatcc
     3181 tcatcgggga ctacgtgctt tcgttccagg atctggcgac ctatttcaac gcgaagaaga
     3241 acgtctcgct cccgcagatt ttcgtgcggg taaacaagga ctccgacgag gtggaactgg
     3301 aacgctcacg gacgcgcgaa agttcgcttg aagtgacctt cggtctcacc gtgcgggtca
     3361 aggccctgcg cgacgtccgg gcgacgctca acggagtcaa gctcaaggcc aacacccagg
     3421 tcgaggcggc gctcgacgat cgcattgtat tccacaacga caccgagatg gagctgcttg
     3481 atctgcggcg ccgcgcccgg gcgatgggcg ggcgctttca actcaaggcg tccaaatccg
     3541 aatatctcgt ttcgaacaat ccgagtttgc tcgaagagga cgacatcctg ctctcgcccg
     3601 ggacggccgg cgatgtgctt ctgaaaattt cctgcgatta cgaaaaacgc atcggcgttc
     3661 tcgaagtgct cgaagccgat cgcccgatca tggtcggtga tgtggcggtc cgaacgaccg
     3721 ccccgctgac cgacggggac accatccgca tcgatgtcgg tcaattcctg cggtgcgatt
     3781 tttcggaacg catcatcgag gaagaacgca acatcattcg cagcctcgaa gtgatcgacg
     3841 ttaatcaccg cttcagcaac agcgacgtcg ctctcgaagg catcagcttc gcgctcaatc
     3901 gcggcgagct ggtctgcgtg atgggcgcga gcggttgcgg caagagtacg ctgatgcgcg
     3961 tgctcggcgg gcagctgcaa ccatcgcgcg gccagatcct gctcaacggc cagccgctct
     4021 acgatagcct cgacacgctg aaacgttacg tcagttacat cccgcaggac gacgccttcg
     4081 acgagcacct gacgatcggc gaaaacctgc agttcgcggc cgcgatccgc tccccgcacc
     4141 tttcacggcg cgaccgcacc cggcggctcg aagccaagct catcgagctc ggcctaagcg
     4201 aacggcgcga cagcatcgtg ggcagtcccg tgaaaaaaat cctgagcggc ggcgaacgca
     4261 aacgactcaa catcggcctc gatatgatcg gctcggccga cgtttacctc tttgacgagc
     4321 cgacctccgg gttgtcttcc aaggactccg agcacgtgat cgagatcatc cgcgggatgg
     4381 cgcacaacaa gatcatcgtc gtcacgatcc accaaccgag ctcgaagatt ttccagatgt
     4441 ttcacaaggc catcctactc gacaagggag gccggctcgt ctttttcggc acgccgagcg
     4501 acatgttgcg ctacttcgcg gaggcggaac atcagcatca attcggagcc gacctcggcg
     4561 cctgcgccgc ctgcgggacg acccggccgg agtttatctt cgatgttctc gagacgcccc
     4621 tgcgcgatct gagcggcgac attatttacg aagaaaataa tcgcggacag ctgatgccgg
     4681 cacgccgcta ttccccggag ttttggcgcg acaaatatga ggcgtttcgc ctgatccagg
     4741 acgtcaagca agttcccttg cggcgcgatc cggtgttgcc cctcccgccg gacccgccgc
     4801 aaaaggcgcg cgagctcttt cgctggcatg acgaatggac gcagctgcgc accctcttgc
     4861 accgcgcctt catcagcaag ttgcgcaatc gcgtgaacct cgttatcacg atcggcgtcg
     4921 cgcccgtcct cgcgcttctc atcgcgacga tcctgcgcta ttccgacagc ggccgctacg
     4981 atttcgcctc ggcttatcac attccgactt ttctttttct caccctgatc gtcgtcatgt
     5041 tcctcggcct gacaaacagc gcggacgaca tcattcgcga ccgcgccgtc ctccagcgcg
     5101 agcgaaacct gaatgtccgg ctgacctatt atgttttctc caaaacggtc tcgttaggcg
     5161 tgtttgccct ggtgcagtgc gttctcttcg tcctgatcgg caactacatc ctcgaaatcc
     5221 gcgggatgtt ctggcccgac ctcgcggtcg tcttcatgac cgcgatgagc ggagtgtcgc
     5281 tgggcctggt cgtctcctcg ctggtggccg acccgaagac ggcggccaac atcgtgccgc
     5341 tcgtcctcat tccgcagatc atcatgggtg gcgcgctgat caagtacgag gacatgaacc
     5401 gcaatctcgg actggtttac acttttgccc gctggttcca cgaacatccc gatcgcgacg
     5461 aaacgcagaa attggacagc cgcctgcagg tgcccttcgt ctgtgaattt atcgcgatgc
     5521 gctggtccta tgaagagctc atcgtggcgc aggcgaaatt gaatcccctc acccgccgcc
     5581 aggacgaagc gcagcggcag atcgacgcga ttgtcgcccg acacgaggga caacctggcg
     5641 atgccaaaag actcgacgac ctgaaagaga cgctcgccgt tctctcgggc ctcgaggccc
     5701 acaccccgcg cgaactcaat caatacctcg ctctgatcga ccgcatcctg aacggcaaga
     5761 cgtcctttga tcgcgccgct ttccagaacg cgaacggtcc cattaccgcc gaacaactat
     5821 tcgtgaatca aaaggtctcc gacttgatcg caaacgcgga gatggagcaa aacgattacc
     5881 ggcgcggcgg caagccaaac gttttcttcg gcgcgcaaaa gcgctactac ggtgcgaccg
     5941 tcaccgtttt cactttcaac accgcggtcc tgatcggatc gagcctcggc ttgctcttgt
     6001 tgttgcactg gattttgcgc cgccaactgg aggtgcgaag aaagtagggc ggacgtttga
     6061 gagagagctg ttctaggcgc cgtccctctg ggaggcgtga gcctgcgcat tgccgcagat
     6121 cgagcgttcg accaacggac gcacgggtcg cgcgacaaac ggcgcgcgca aaattttgtg
     6181 gacgcagcag ttcgaggaac cgagtagaat cgacttgata tgaaaaaaaa tccacctaag
     6241 ccgcgcttga tcgtggcggc gagcgagaac gatcccgaca tgctttacgc gacccgtttt
     6301 ttcgcgcctg acgccttcat tttccttgag cagaacggcc ggcgtattct cgtgctgagc
     6361 gatctcgaga ttgaccgcgg gcgcaaacaa gccgcggcgg acgaaatttt accctacagc
     6421 gcgtttgaaa aagaagtgca agggacgagc gccaaagcgc cagcctatga aaaggtcgtg
     6481 gcgcatttcc tcacaaaaag aaaagtgcgg tcggcagtcg tgccggccaa tttcccactc
     6541 ggtctggcgc gtgaaatcga gtcgaaaaaa atcagcctcc agccgacgaa cggcctcttc
     6601 tggccggagc gtgaaaccaa ggccgaaccg gaattgcgct tgctgcgcaa ggcgctcgag
     6661 atcacggaag ccggcatggc gcgcggtttc gaggtgctca aacgcgcgca gccgggcaag
     6721 gcgcaaaaat tgcaatggtc gggcgccgtt ctgacctccg agattttgcg tgccgagatc
     6781 gacaccgccg ttctgcgcgc gggaggtttg cccgccaaca cgatcgtcgc cgggggcgat
     6841 caggcctgcg atccgcatga acgcggacac ggcccgctga aggcgaacag tctcatcatc
     6901 atcgacattt ttccgcgctc ggcagcgacc ggttactatg gtgacctgac gaggacggtg
     6961 gtgcgtggac gcgcgagcga cgagcagcgc cgcctctggg aaacggtcaa tgcgggccag
     7021 gccctcgcct taaaaaaaat gaagcccggc gtcgatgggc tgaagttgca caaggaagtg
     7081 aaacaatttt ttaccgatcg tggctatccg accgaagtcc gcgacggccg gcagacagga
     7141 ttttttcatg gcaccggtca cgggctcggc ctggagattc acgagttccc gcgcttccaa
     7201 aagaccgtct tcaaacccgg ccaggtgctg acggtggaac cgggactata ttatcccggc
     7261 ctcggcggaa cgcggacgga agatgtggtc gccatcacga cacgcgggat tcggatgctt
     7321 tcgcgctgcg aaaatcggct ggagttgtag acgccgctct ctggacggcg cgggtttgcg
     7381 cgtgatcgcc gattcgacac ccgcgcttcg ccgagcgaag cgtctatcat aaccaagtgt
     7441 ccaactgccg caccacaatg aacgaacaac gcgctcaggc gtctaatcca aagacgattc
     7501 ttatttaatt gatgtacgca ccctattacg gcgggctttt ctcgtcgaac tatctcatcc
     7561 tcgtcggcat tccgcttgtg atcgctttat gggcgcaatt tcgcgtcagc ggcgcgttca
     7621 agaaatgggg cgcggtgcgc gcgagttcca acattaccgg cgcggaagcg gcccgcgaga
     7681 tcttgcaggc tgctaatatc ggcgacgtca acgtggtcga gatcaacgat tacctcggtg
     7741 atcattacga tccaacgcag aagcggctct gtctctcgag caacgtctac aacacgccat
     7801 cggtcgccgc gctcggcatc gccgcgcacg agaccggcca cgccatccag cacgccaaag
     7861 cctacgcgcc cctcaaggca cgcatggcgg tcgtgccggt cacggggatc gcctcgcgca
     7921 ttctgccctt cgccattttc ggcggcctct ttttccacat caccgggctg atcacgattg
     7981 gcatctactg ctatctggcg ctgctcgttt tccaattgat cacgctgccg gtcgagttcg
     8041 acgcctcgcg tcgggccaaa atcattctcc agcaaatggg catcattcag cccggcaacg
     8101 aagccctcgg cgtgaacaag gttctcaacg cggcggcctg gacttacgtc gccgccttcg
     8161 tcgccaccct cggcaatttg ctctggctcc tttcggtgcg cgatcggcga tgatacctgc
     8221 cgttgcgctg ccggcccccc gccgcctaac cgatttgcta tcgtcgggtg ctcccgcgcg
     8281 tgctaaacgt agtcgggagg cgatggacca accgcgaacc gatgaagatc aggcgctcgt
     8341 cgcgcgcgcc caatcgggcg acgccgcggc cttcgatgat ctcgtggtga aatattcgcc
     8401 gcgcctctac ggcctgattt acaacatgac ctcgaaccac gaggacacga acgacatgct
     8461 gcaggatgtc ttcgccaagg cctaccgcgc gatcaagggc ttccgcggga agtcgtcgtt
     8521 ctacacctgg atccacacga tcgcggtcaa catgacgctc aattttctca agaagcgcgg
     8581 ccggcgttat cacctcagcc tcgacgatgt cgacgcgagc atccagaacg acaaggaatt
     8641 tatcgagctg acctcgacga gcagcccggt gcgagaagcc gacctttccg agctccagcg
     8701 aagattgaac gaggcgatga tgaaactgtc tgatgaacac agggccgtcg tcaccatgtt
     8761 tcacattcaa ggaatgccac acgcggagat cagtaaaatc cttggcgttt ccgaaggaac
     8821 agtgcgatcg aggctcttct acgcgaatcg ccaactgcaa aattatctgg acgaattccg
     8881 caagaacccc gttggttaga actcgcagcc catggacaac ttcgacgaaa acgaaatcgg
     8941 aaaattgctt cgcttgaaaa agtacgagca accgcctccc ggttacttcg aaaatttcct
     9001 ccatgaattt catcgccgcc agcgggatga gcttctgcgc cagccggtct ggagcgttct
     9061 ctggcagcga ctttccgatt ctcttttccg gttaaacatc cggtcgctca cctcgtatcc
     9121 ggccgccgtc gccgcggtcc tggtctgcgc cgcagtcatt tcgttgaagg tctaccaggc
     9181 gccgtcatcc gccggcgacg cgatggtcga tacgagcacg ctcccatcca atgaaagcgt
     9241 gatcgatcaa atgccgagcc agcgcgtggc cgattatttc acgcttagct cgtcgcttcc
     9301 gacgcgccgg ctcgggcccg atctctcccg tcagatgaat gaaagcgcct acactcaccg
     9361 agccacgacg ccgccgcgct acgtgctcga ccggttgccc gtgagttatg agccgtcttt
     9421 caatttctag ccaggcgtcg cgctcgtttc tttcccgcgg atgcacggcg ggtctcgcca
     9481 gtctctgcgc actcgttagt ctgagcctgc ccgcgctccg cgcgcaggac gatacggcgg
     9541 gcgccatcag ccgcgaagtg aagtcgctct tcgaacgctg cggccaggcg atcgttaaaa
     9601 ttcacgcagt cgacgaacac agcgagcttt ccgggactgg cttttttgtc gacccgaccg
     9661 gcaccattta tacctcctat tccgtcggcg gcgaggcgag taatttcacc gtccagttca
     9721 acggaaaaaa atatcacgcc acccaattga tggccgatct gcgcagtggg atcgcgcttt
     9781 tgaaggtgga cgcggcgacg cccttcctcc cgatcggcaa atcagccgaa ctcggcctgg
     9841 ccacgcctgt cgtggccatc ggctatccgc tcgatttgcc ggagtcgccg agcttcggca
     9901 tgatcgcggg cttcgaccgg aaatatctcg gccgttattt tgcgaccacg cacctgcgga
     9961 tcaatctccc cacccagcgc ggcgaagcgg gcgccccgct cctcaatttc aaaggtgaag
    10021 tcgtcggcat tcttgtcagc agtgtggaca gcggctcggc ttgttacgcc ttgccaatcg
    10081 acgccgcgga aaaaattcgg cgcgattacg tgcgcttcgg cgatgcgcgt cacggctgga
    10141 tcgggatcga cgtgcatgcg acagagaatg gagtcggagg ttccaacgcc gagatgacgg
    10201 aaatccatga aggcacaccg gcggccggct cgggcttgaa gacgggcgac atcctgttgc
    10261 aggtcggccg ggttgcggtg cacgaaccgg aggatgtgat cgacgcctcc ttcttcatca
    10321 gcgccggcga ttcggtgccg atcaccgtca tgcgcggaaa ggaaaaactt actttcaatg
    10381 tccaggccgc atccgcgaaa cctgaggcaa tggcgctggg accgagcaag aagtcggctg
    10441 tgcctctgaa gctggaggcg actccgaggt agcctgcctt agagcatccg gcgaaaagcc
    10501 gcctcatcga ccacccgcag gccgagctgg cgcgctttct ccagcttgct ccccgcttcc
    10561 tcccccgcga ggacgaagct ggtcttcttg ctcacgctcc cgctcacctt gcccccgtgc
    10621 tgcacgatct cctcggcgat ttcgtcgcgc ggcttgctta acgttccggt gattacccag
    10681 gtcgtccctt tgattttcga atcggagggc ggcgcggctt ttcggtcgct cgttagacgc
    10741 aagccggcgg cccgcaactt ctcgatcatc tcgcgattcc ctggctcgcg aaaaaactgg
    10801 gagatacttg cgccgacgac ttcgcccaca tcggggatgc gctggaggtc ttccggcgtg
    10861 gctgccagca acgcgtcgag gttgggaaag tgatcggcca gggcgcgcgc cgcactcacc
    10921 ccgacatgca aaatcccgag gccaaaaagg aggcgccaga gcggctgtcg cttgctgcgc
    10981 tcgatcgcgg cgcagagatt cgcgacactt ttctcgccca tccgctcgag ctcgcccagt
    11041 tgcccgctcg ttaggcgata gatatcgctg acctcgcgca ccagcttgtg ctcgaccagt
    11101 tgctcgacca tcgcctcgcc caggccttcg atgtccatcg cgccgcgcgc cgcaaaatgc
    11161 tcgacccgcc gtttcaactg cgcgggacat tgcgcgttta cgcagcgcac cgcgacctgc
    11221 ccctcgtcct tcaccacctg actgccgcag accgggcact tcttcggtat ccgaaatttt
    11281 ttctcggaac cagtccgcag atccgtgcgc acgctgacca ccgccgggat cacttcgccc
    11341 gctttctcaa tcaccacggt gtcgccgatc cgaatgtctt tgcgcgccac ttcctcttcg
    11401 ttatgcaagg tcgcgcgggc cacgcggctc cccgaaacca ccaccggttc gagcgcggcg
    11461 accggagtga gcacgccggt gcggccgacc tggatgagaa tatcgagcag cttcgtctcg
    11521 acccgctccg cttcgtactt gaaggccatc gcccagcgcg gcgccttggc ggtcatgcct
    11581 aacaactcgc gctgggcgaa ggcgtcgacc ttcaccaccg cgccgtccgt ttcgtaaacg
    11641 aaatcgtgcc gcacctcttc gagctcgcga atcgcgcgca ggattccctc aactgaatcc
    11701 gccggccacc agcgttcgct gcagggcagg ccgagtttct tgagcaacgg gaaaagcttc
    11761 gaatgatgcg tcaactccat cccctcgacc gcacccgtcc cgtagaagat cacgcccagc
    11821 gggcgttgcg ccgtcatcgc cgggtcaagc tgcttcaagg agccagccgc ggaattgcgc
    11881 ggattcgcga agaccggcag cccggcttcg ctccgttcgg cgttgagttt gacgaaaccc
    11941 ttctttgtca tgaagacttc gcctcgcacc tcgaaaagcc gcggggcgtc gcctttcagt
    12001 tgtttcggga ccgagcggat ggtgcggatg ttctgcgtga tatcgtcgcc cgtggtgcca
    12061 tcgccgcgcg tcgccgcata tttcaggcgc ccgtcttcgt agagaaggga aatggccacg
    12121 ccgtcgacct ttggctcgac cacgaccggg atcttctcgt taggcaaaag cttctcgagg
    12181 cgccggtaga acgctccgac ttcttcttcc gaataggtgt tgtccaggct gagcatcgga
    12241 acgcgatgcg tgatctgcgc gaaggccttg agcggctctc cgcccaccca gcgagtgggt
    12301 gaatcggggg tgatgagctg tgggaagcgc tcttcgaggt cgaccagttc gcgatagagc
    12361 cggtcgtact cgcggtccga aatggtgggc gcggcttcct catagtagcg ccggttgtgt
    12421 tcctcgattt ccgcccgcaa ggctgcgatg cgctgggccg ccgacgcttc cgtttccgcc
    12481 atgagcgatc atacaaacgg cgccgcaaac ggcaatctcc ggtagccgct cgccgcaacc
    12541 agttgcaact tccaacgaat ggagcacgcg atggagagaa ttctcggcat cgagggcggc
    12601 ggcactaaaa cctcgtggat gctcttcgac gcgcgcggtg gcgaattgca actcctcgat
    12661 gacggctcgc tggctcccac gaattttagc ctaacgagtc gcgaaagaat cgtgcagatc
    12721 tggcgggaga tgccgcgcaa cgtcgaggcg gtcggcgcct tcctcgccgg ctgcgggacg
    12781 cccactgatc gcgcagaact aacgaaactc tgcgccgaag tctggccggg agccgcgatc
    12841 gtaaccggca gcgatcgcga atccggattc gcttcggcct ttggcgaagg cgacggcatc
    12901 gccgtaaatg ccgggaccgg atcgtccatc accggccgcc gagagggccg tttcgaacgc
    12961 gccggcggct ggggtcacgt cctgggcgac gccggcggcg gctatgatct gtcgctggaa
    13021 atgttgcgct tcgtgctgcg cgactacgat ctgcaccggc gcggcagcga actcgcgcag
    13081 gaagtgttgc gcgccctcgg cctgaacgac ctgaacgaac tcgttcgttg ggcgcagacg
    13141 gccggcaaga tggaaattgc aacgctcgcg ccgctcgttt tcgcagccgg cgccgccggg
    13201 cacaaggaag tcaacgacat cctgtgcgcc ggcgcccgcg tcctcgccga gtacacatgc
    13261 gcggtcgcgg atcggttgga atttcgcgcc ccgccggtcg cgctgctcgg tggattattt
    13321 cgcggacatt ccatctatgt gtcggcctac cgccgggagt tggcggagct atcgccggtg
    13381 gccaatgtgt tggtggcgga aaaaccttcg gcgttaggcg cagcctggct cgcgctcgcc
    13441 caaagaccgc cgctcgcgcc gcatctggaa aaaccagcgc cggaaatcgc ggcggccgcg
    13501 acggaaggaa tcaacccgcg atcgctggat ctcgagaacc tttcgccccg ggagttagtc
    13561 gatctcttcg tggcagaaga gaagtttgtg caggaagcgc tgcgcgcggc ggcggaccga
    13621 ctggcggagg gcgtctccct ggtcgcaacg gcgttgaaaa gcaagggccg ccttttctat
    13681 gtcggagcgg gcacgagcgg tcggttgggc gtgctcgatg ccagcgaaat cccgccgact
    13741 ttcggcgcag cgccggagct tgtgcaaggg atcatcgccg ggggcgccgc ggcttttcag
    13801 cgcagcgcgg aaggcgcgga agacgacggg cacgcgggcg ggctcgcgat cgtcgaacgc
    13861 ggcgtgaggg caaacgacgt cgtctgcggg atcaccgcca gcggccggac accgttcgtg
    13921 ctgggggcgc tgcgccgcgc gagggagcgc ggcgcgcgca ccctcctgct gacctccaac
    13981 ccggcggcca gctccttgcc taacgaattt gatctttgca tcgttctccc gaccgggccg
    14041 gagttgctgg ccggctcgac gagactgaag gctggcacgg cgacgaaggt ggcgctgaat
    14101 attttgtcga ccggggcgat ggtccagctc gggtgtgtgc gaggcaacct gatggtcgag
    14161 ttatccgcga cgaaccaaaa gttgcgcgcg cgcgccgtcc gcctgctggt gcaactcacg
    14221 ggttgcgatg ccgagacggc gagggcgcgc ctggcgtccc acggctggaa tttgcgggcg
    14281 gcgctggccg gcggggacgc ggccggctaa aagccttcgt tctcgggcag catgtcctga
    14341 gtgaagtggg ggttggccag ccgatctttg atcgctacct tggttacggg cgaagaattg
    14401 tcggcttcgg cggccacggc gtgctgcgac gtaatcctgg ctttgggcgt tttgtgcctc
    14461 cacgcaagct cgttgaattc gtcaccgatt tcgtaaacca ggaagacggc gcgcgtcacc
    14521 atctgtcgac gaaccttgtt gtcccggtcg cgcagttcca gtttgtagat gccatccacg
    14581 cccagcgagt cggtgagcgg ggtggaccta aaggtcttgt tcacgaccac tccgctcgcc
    14641 gaccaactgc caaacggtga accctgagcg acggcgccgc cgagcagaat gcaaaacgcg
    14701 aaccgaaaga aattcttgca gagcatcacg gtggagcaac cgatcaaccg agaccggcag
    14761 ccatccgcct ggtatgcaat ttccgcgccc gaagggcgaa aaccccggaa attttcaggt
    14821 cagcgaaagg ttttcttcag aaacaagcac gcggatagac cccgcggcac gaagcaaatt
    14881 gaaacttaac cggaggaaaa atatgttgtg gactatattc gttattctgt tggtgctctg
    14941 gctactcggc ttggtgagct cctacacgct gggtggattc attcacatcc tgctcgtgct
    15001 ggccatcgtg gtgctgatta ttaatctcgt ctccggcaga cgcagcattt agcgcgtgaa
    15061 accggcgggc attatcggtc tcatcctcat cgtcgtcggc atcatcgcgc tggcttatgg
    15121 tggattctcc tataccaagc gcgaaaagat catcgacgcc gggccgctcc aggtgagcgc
    15181 cgaccgggag cacaccgtgc ctttgccgcc ggtcctcgga gccctcgcgt tggccggcgg
    15241 gatcgtgctt gtgctcgcgg gcaacaagaa atagcggcgg cgcagcgtct cgccgctgtt
    15301 tttggagccg gggcgtcgac cccggtcgcc gccagccaac gacacattcc ctgacctggg
    15361 tcagcgctct cggcgacaac ggcgacacta gatatggatc gcgtggccga aagcctgacc
    15421 caccgcttca tggacggatt cgctcaaggt cgggtgtgcg tgaatggtcg cctcgatctc
    15481 ttcataggtg gcctcgagcg tgatcgccaa cccgagttcg gcaatcatct ccgttgcctc
    15541 gggcccgata atgtgcgcgc ccagaatttc accgtgcggt tcgccgatga ttaacttcac
    15601 gaagccctcc gtctcgccga tcgcccgcgc tttgccgctc gcgctgaagg ggaattttcc
    15661 gatccggaat ttcttgcctg actcttgcgc ggcgcgctcc gtcaggccga tgctcgccac
    15721 ttgcggctgg caatacgtgc agccgggaaa ttcacggatc ttgcgcggct cgatttcacc
    15781 aaacatgcct tcgaccgcct ggatggcttc ccagctcgcg acgtgcgcga gccagggcgg
    15841 cccgatgacg tcacccgccg cgaagactcc ttgagcgtcc gtttcgtagc ggtcgtcggt
    15901 ctggatgtaa ccgcgcccgg tcaattcaag tttcagcttt ccgttaggca aaagcggcct
    15961 gaccccgatc gccaccaagc aaacatcggc ctcgatcgtt tcggcagatt ttccttccac
    16021 cgcgatccgc acccccgttt tcgtcgtctc ggtcttcgcc gtcttcgtgt tcgtcaggat
    16081 tttgatcccc tgtttcgtta gggctttttc cagcgcctgc gacacttccg tgtcctcgac
    16141 cggcaaaacg tcaggcagca tctcgatcag gatgaccctg gtcccaaagg cgttgtaaaa
    16201 ataggcgaac tccaccccaa tcgcgcccgc cccgatgatg accatgctct tcggttgcgg
    16261 atcgaggatc atcgcttcgc gactgccgat cacttttttg ccgtcgaacg gcagtcccgg
    16321 aagggcccgt gaaagcacgc cggtcgcgac gagaattttt gccgcggcgt gcgtttcggt
    16381 ttttccctcg gcgagtttca ccagcacttc gcccggtttg tccatcgagg cttcgccgcg
    16441 aatgtaatca accttgttct tcttgaagag gaattcgatc ccggcggcgt tcttgtccga
    16501 gacgtcgcgc gaacgcttga tcaccttgct ccaatcgaac gagagcccgt cgatcttgaa
    16561 accgaagtcg gccgcgcggt gcttcatgag atgatagagc tcggcgttgc gcaggagcga
    16621 cttggtcggg atgcagcccc agttcaggca ggtgccgccc gcccgctctt tctcgacgca
    16681 ggcgacgcgt tccccaagtt gcgcggcgcg gatcgcgccg acatagccgg cggggccgcc
    16741 gccaataaca accaaatcgt agttcatgga aaatctcgaa ggcgcgatcc tgcggcccgg
    16801 cctagtcgtg ctcgtcaggc gaacggcccg ttgtcgcgat caactgttga aagcgtggat
    16861 cactccgaat tgggtcccag cccggatcga tcttcaagag cgggactgtc gtgccgttgg
    16921 gagtggagag cgaacgcttg atcaactcca gggcccgctc gttttccccg gcccaggcca
    16981 gcacctgggc ctggccgatc gtcacctgcg gaccatcgaa ggcgtcgcgc gattcgggga
    17041 gcaattgcgc ggcacgatcg gcttcggcaa tcccgccctg cttgtcccca atccccacga
    17101 gcagttgggc gagcagcagg tggcgcgacg cgtcatctgg gctttcgcgg atcagccgct
    17161 ccaccatcgg ccgggcgcgc tgaaaggcag cccgcgcctt ctcgttctcg tgcatgtaaa
    17221 gatagatcat gccttcggag aaagatttcg ggaccggcgc cgttcccatg ctgtgcagga
    17281 tttcgcccgg acaattttga agcacctgca aggcggcggt gaattttcgc tgcatgatcc
    17341 acacattggc ccgaacgaac gaaatcaatc cgtccgggtc aaaaccgtcg ggcgcgttgg
    17401 cgaggaggct ttccgcgaca ctaaaatccc cgcgcccggt cagcgcgatt tgtgccttca
    17461 ccccccgcaa cgaaaacgaa tccggcgcag cttgtagtcc gcggtcgacg gtcctgtcgg
    17521 cctcatcata gcgccgcagc gcaatgtagt tgaaggccaa attcaggaga aggtcggcgt
    17581 ccttgggatt gagcgaaacc gccttctcaa agttcgccgt cgattcattc cacttgccct
    17641 gccgtcgctg gatggccgca atcgccttca gattttctgc atcgttaggc aaaccatgtt
    17701 gcgccgcagc aaattccccc agggcccgct cgtagtcgcg ttgtgtccaa tagtagtaat
    17761 agccgagggc cgcgtgcgtc tccggtagat tgggctgcaa acgctgcgcc tcttccgccg
    17821 ccacgcgtgc tttttcctgg cgcgcggaag tcggatcgct cgtgtgataa atccagcttt
    17881 gcaaacagga aagttccgcg aaggcgaggg caaacttcgg atccaattgg atcgcgcgtt
    17941 catagagttc ctcggccttt tggatatcgg tctcaaggtg atcgggtcgc atctgcagat
    18001 cgtgcgcctc cacaaaggcc agataagctt cgccgttctc cgtcggtttc tgcgatacac
    18061 gcgcttcctc ggtaggcgaa agccgggcct gaagggcacc cgcgatttcg tgcgcgagat
    18121 cagtctggat cgcaaagaca tcggtcaatt cgcggtcgta ttcgttcgcc cagagatgct
    18181 catcgttctc cgtgttgatc agctgcacgt tgacccgcac acgtttgccg tcgctgcgga
    18241 cgctcccctc gagaatggca cccgcaccca acgcctttcc gatctcgcga acgttgcggg
    18301 tggagccgcg ataccccatc acagaggtac gcgagatgac tttcaaatcg ctaatccgcg
    18361 agagcgtcgt cagaatgtca tcctggatgc cgtccgcgaa ataagcgttg ctcttgtcgt
    18421 cgctgaaatt ttcgaacggc aacaccgcga tcgacttgtc caccttcttc gcggccacgt
    18481 gcggcaaaag aaagaatccg gccacgatcg ccaccgttag gccgaccgcg cctaacaaaa
    18541 agacgttgcg ccggcggtgc ggattggtcg ggtcgccggc cgccggaaga gcgggcgtcg
    18601 tctggatgcc agttgaggta atgtcgaaag cccaagccag gatgagcgcg atgggaaatc
    18661 cgaccagcaa gagcacaatg acaaggcgca gcgaccagtt gggcaattcc cacgccggaa
    18721 aaaccgccga cgcgagttga atgatgccgc cggccgcgat cacataggca acggcgacgc
    18781 ggtaaacctt gcgccgcttc agttcgtcga aaaag
//
LOCUS       DATMNS010000069        10367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_802506, whole genome shotgun sequence.
ACCESSION   DATMNS010000069 DATMNS010000000
VERSION     DATMNS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 10367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10367
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_802506"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /locus_tag="VNW28_02605"
     CDS             <1..889
                     /locus_tag="VNW28_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA08843.1"
                     /translation="DNISFEIEEGELVGFLGPNGAGKTTVLKMLSGLLNPTSGQARVL
                     GFVPWERRDEMKRQFSLLMGQKNALWWDLPAQESLELNRAIYGIDRDRFKKVVGGLSE
                     LLEVQDKMNVMVRELSLGERMKMELISALIHEPRVLFLDEPTIGLDVVSQKRVREFLR
                     LYNQEHRIVTMLTSHYMQDIEELCHRVIIIDHGKIFFDGPLSAIIDRFSGHKVISLTY
                     ADKQARDFSELGEVVEQTPVSVQLKVPRAKVTEVCRQLLSACSVSDINVQELPVEDVI
                     RQLFGERRGENRAAQTPPA"
     gene            complement(896..1168)
                     /locus_tag="VNW28_02610"
     CDS             complement(896..1168)
                     /locus_tag="VNW28_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08844.1"
                     /translation="MRKIKLSGREHAVLKALGFASAVPGAEVQDYTQMEPDDLTDVLN
                     ALLAAGYVESKPYREQVTLAEMPTVEFEANPAYVHELRAAMARSWV"
     gene            1349..2398
                     /locus_tag="VNW28_02615"
     CDS             1349..2398
                     /locus_tag="VNW28_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HXA08845.1"
                     /translation="MPVSEDQIKEALKAVKYPGFSRDIVSFGIVKGIDLRDGDVTVQM
                     ALATNDPNVPQTIKNEAETALRNLVGVRAAKVKIDIHAPPAGSGSAGVGATRISGIKH
                     VVAIASGKGGVGKSTVAANLAVALEQSKAAVGLCDCDIYGPSISLMFGSRERPMATED
                     NRIIPIEQYGLRLMSMGFLLDDAAPAILRGPMVTRYTQQFLRQVEWGELDYLVLDLPP
                     GTGDIQLTIVQTVALAGAIIVTTPQEVALIDARKAAAMFEKVNVPVLGLIENMSYFIS
                     PSDGKRYDIFGSGGGEREAKRIRVPLLGQIPIDIATREAGDRGMPVMAEAPESPVGAE
                     FAQVARRLREILSTT"
     gene            2501..2737
                     /locus_tag="VNW28_02620"
     CDS             2501..2737
                     /locus_tag="VNW28_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08846.1"
                     /translation="MNENAGKASEDEHFLKSSVLYKEFLAEREEVLKHKWIESEKAGS
                     DIGFEKALLDWIVKHRSNWRERRVRETRPTKTST"
     gene            2784..3359
                     /gene="pyrR"
                     /locus_tag="VNW28_02625"
     CDS             2784..3359
                     /gene="pyrR"
                     /locus_tag="VNW28_02625"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HXA08847.1"
                     /translation="MSKTRPTNESDAVRIFDAETMQRALRRIAHEIIERNRNLSALVL
                     VGIPARGVELARRLAAVIEAIEKVRVATGVIDVSMHRDDIGTRRALPVVKVSQLPLPL
                     EQRTVVIVDDVLFTGRTCRAAMDAIGSFGRPACIQLAALVDRGHRELPIRPDFVGKNL
                     PTAFAERVRVRLENIDHQPDGVWLLKPEANE"
     gene            3352..4293
                     /locus_tag="VNW28_02630"
     CDS             3352..4293
                     /locus_tag="VNW28_02630"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HXA08848.1"
                     /translation="MNSDKGRWTRKDLLGLRELSVEEINLVLETADAFKQVGTREVKK
                     VPALQGKTLINLFIEPSTRTRTSFELAAKRLSADIVNISAAASSLTKGETLKDTARVL
                     EANHADIIVLRHSSAGAPQFLASRIASSVINAGDGAHEHPTQGLLDLYTIREKKGAVA
                     GLHVAIIGDILFSRVARSNIFALLKMGARVTLVGPSTLVPREFEQLGVTVSHNLEDVL
                     PTVDVVNLLRIQHERQRKEYFPGLGEYIRLFGLTKARARLLRPDCLIMHPGPINRGVE
                     LDSELADGAQSVILDQVTNGLAVRMAVLYLCGGIATS"
     gene            4290..5570
                     /locus_tag="VNW28_02635"
     CDS             4290..5570
                     /locus_tag="VNW28_02635"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXA08849.1"
                     /translation="MNTLIRNARIIDPANARDEVGDLLIVDGRIASLSQLSTFNSQPD
                     EIDAADLVVSPGLIDMHVHFREPGFGWKETIESGARAAAAGGFTTVVCMPNTSPVADS
                     PSTIAWIKDRAIEVAAVHVLPTGAISKNLAGEELAPIGSLAQAGVVAITDDGRCVQNN
                     ELMRRAVEYARMVGVPVLDHCQDYNLVGHGVVHEGYWSTLLGLPGWPAAGEEAIVARN
                     ILLAELCDHRIHCQHISAAGSVRLLREARSRGVKISGEVCPHHISLTDEAIQNFDTDC
                     KMNPPLRAQRDIDALLEGIADGTLEILASDHAPHAKFEKEVEFDAAPFGIVGLETELG
                     LFLDLLVHRHRTIALPRLIEMLTVNPAKLLGLKCGTLSLGAPADVTLIDPDLEWTVDA
                     GAFQSASRNTPFHGWKLKGRAVRTIVGGKTVWQL"
     gene            complement(5571..6020)
                     /locus_tag="VNW28_02640"
     CDS             complement(5571..6020)
                     /locus_tag="VNW28_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXA08850.1"
                     /translation="MDVSGTIERILSQKNREIWSISPDATVYEAIALMAEKNVGALLV
                     VENGKLVGIVSERDYSRKVMLKGKTSRTSYVREIMTTELTTAHPRETVEECLRFMTEK
                     RIRHLPVVADGSLRGVVSIGDLVKQVISSQSATLEQLRDYISGGYPS"
     gene            complement(6061..7362)
                     /gene="sufD"
                     /locus_tag="VNW28_02645"
     CDS             complement(6061..7362)
                     /gene="sufD"
                     /locus_tag="VNW28_02645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HXA08851.1"
                     /translation="MAGILEGIQRHGDDQVPAWFGDQQQAAWTQFQSLPMPVRTDQAW
                     RFSNVGLLDLSPYSTAGRLSEADSHEILERSGELDETSARLVFADDHFLERDVLSENL
                     RKAGVIFQPLERAMIEHEELFRRHFMSQPAALGSAKFSALHEAFVRSGTFLYVPRGVE
                     VELPLETFHWLHTENAAIFPHTLVVAEDLSKITLVEHFRSTDPQRAGFACGVNDLIVG
                     PGAKVTYICAQDWSDKTLSIQINATTVARDARALNLHLGLGGKYSRLESLSRLAGEGA
                     HSDMLAVSIGNEAQEFDARTLQDHASPHTYSDLLFKNALNGRSRNTFGGLIRVEPHAH
                     FTDAYQKVRNLLLSDDAEANSMPGLEIMADNVKCSHGATSGQLDDDEMFYLLARGIPA
                     PVARELLVGGFLNEVLERLPDPVLVAKLEAMIARKFAHRAC"
     gene            complement(7481..8890)
                     /gene="sufB"
                     /locus_tag="VNW28_02650"
     CDS             complement(7481..8890)
                     /gene="sufB"
                     /locus_tag="VNW28_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXA08852.1"
                     /translation="MVKENKTLDIERNLGNFAYPETHTHDAGIGLSEKTIHYISDVKQ
                     DPDWVREFRLNALRTFLSKPMPTHWASKDLEAIVFDNIRYYLSGGTQPKRSWEDVPDD
                     VKRTFERLGIPEQERKFLAGVEAQFDSEAVYSNIKKAVGEQGVIFVGSADGLKHHPEI
                     FRKWFGKVIPTGDNKFSALNSAVFSGGSFIYVPPGVKVKHPLQAYFRINAENFGQFER
                     TLIIVDEGAELTYMEGCTAPKFNTATLHSAVVELVALKGAKLQYITVQNWSNNVFNLV
                     TKRGLAHEEAEVKWIDCNIGSRLTMKYPGVVMKGRKARGEVISIALAGDGQHQDTGAK
                     MVHAADETTSNIISKSISIGTGRATYRGLVHVPKHLKNCKNNTECDALLINANSRTDT
                     YPAITVRGTGNAVQHEASVSQVSAEQIFYMQQRGLTKAQAMSLSVNGFVNDLVRQFPM
                     EYSVELKRLIELEMEGSVG"
     gene            complement(8904..9668)
                     /gene="sufC"
                     /locus_tag="VNW28_02655"
     CDS             complement(8904..9668)
                     /gene="sufC"
                     /locus_tag="VNW28_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HXA08853.1"
                     /translation="MDQLVIQDLHVSIADQEIIRGLSLAIPKGEVHAVMGPNGSGKST
                     LAKVLAGHPDYVVTSGSVLMDGENVLELEPDERARRGLFMAFQYPSEIPGVTIANFLR
                     AAVQARLPEGEELEATDYYAQLYQKMDLLNMDRAFTSRAVNEGFSGGEKKRNEILQMA
                     MLNPKYAVLDETDSGLDIDALRVVAHGVNSLRGPNLGVLLITHYQRLLNYIVPDHVHV
                     MVRGRIELSGDKDLALQLEEKGYDWVREDITEPALA"
     gene            9797..>10367
                     /locus_tag="VNW28_02660"
     CDS             9797..>10367
                     /locus_tag="VNW28_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXA08854.1"
                     /translation="MDDPHAADQDRQIRAFVLCGGLGTRLRSVLSDRPKSMAPVAGVP
                     FLQLLLEDLRSQGILEIILGTGYMSEQVEAFFPQGENIGLHVRYSRENKPLGTGGALK
                     LAESLLSDPVVVLNGDSYVEWSLAATVELFRKKDAAIVIVLQTVADVSRYGSVTIEAD
                     GCVTEFVEKGTRTGPGLINAGVYLLRREIV"
BASE COUNT         2011 a         3091 c         3145 g         2120 t
ORIGIN      
        1 ggataatatc tctttcgaaa tcgaggaggg cgaactggtc ggcttcctcg gcccgaacgg
       61 cgccggcaaa acgaccgtgc tcaagatgct ctcgggtctg ctcaacccga cctccggaca
      121 ggcgcgcgtc ctcgggttcg tcccgtggga gcggcgcgat gagatgaaac ggcagttcag
      181 cctcctgatg ggccagaaga acgcgctctg gtgggacctt cccgcccagg aatcgctcga
      241 gctcaaccgg gcgatctacg ggatcgaccg cgaccgtttc aagaaagtgg tcggcggcct
      301 gagcgagctg ctcgaggtgc aggacaagat gaatgtgatg gtacgcgagc tctcgttagg
      361 cgagcgaatg aagatggaac tcatctccgc tctgatccac gaaccgcgcg tcctttttct
      421 cgatgaaccg accatcggac tcgatgtcgt tagtcagaaa cgtgttcgcg agtttctccg
      481 tctctacaac caggagcacc gcatcgtcac catgctcacg agccattaca tgcaggacat
      541 cgaagagttg tgccatcggg tcatcatcat tgatcacggc aaaatctttt tcgacggacc
      601 gctgagcgca atcatcgacc ggttctcggg tcacaaggtt atcagcctga cttacgcgga
      661 caaacaggcg cgggattttt ccgagctcgg agaggtggtc gagcaaacgc cggttagcgt
      721 gcaactcaag gtgccgcgcg cgaaagtgac cgaggtctgc cggcaactct tgagcgcttg
      781 ctcggtctcg gatattaacg tccaggaatt gccagtcgag gatgtcattc gccagctttt
      841 cggcgaacgc cgcggggaga atcgagccgc ccaaacgccg ccggcctgat ccgcttcaga
      901 cccacgaacg cgccatcgcg gccctcaatt cgtgcacgta ggccggattc gcctcgaact
      961 cgaccgtcgg catttccgcg agggtcacct gttcgcgata gggtttgctc tcgacgtagc
     1021 cggccgcgag cagcgcattc agcacgtcag tcaggtcgtc cggctccatc tgagtataat
     1081 cctgcacctc cgctccgggg acggcgctgg caaaacccag cgctttcagc accgcgtgtt
     1141 cgcgcccgct gagcttgatc ttgcgcatcg gggtcattgg aaagcaggtc gcataccgga
     1201 atgtggaggc ttgcacgcgc gtgtcgcccg cagaggctta cgccccgctc attcctaccc
     1261 agctgtgcgg ccgacacggc tgccgcaaca ccggcgcgaa atgcgccagt ctttactccg
     1321 cgaacgcccg ccgtagcttc ccagttccat gcctgtttcc gaggaccaga ttaaggaggc
     1381 gctcaaagcg gtgaaatatc cgggctttag ccgcgatatc gtctctttcg ggatcgtcaa
     1441 agggatcgac ctgcgcgatg gcgatgtgac ggtgcaaatg gcgctcgcga cgaacgaccc
     1501 caatgtgccg cagacgatca aaaacgaggc ggaaaccgcg ctgcgcaatc tcgtcggggt
     1561 gcgcgcggcg aaggtcaaaa ttgacattca tgctccaccc gccggcagcg gcagcgccgg
     1621 ggtcggcgcg acgagaatca gcggcatcaa acacgtcgtc gccatcgcca gcgggaaggg
     1681 cggtgtcggc aaatccaccg tcgcggccaa tctcgcggtc gccctcgagc aatccaaggc
     1741 tgccgttggc ttgtgcgact gcgacattta cgggccgagc atttcgctca tgttcggctc
     1801 acgcgagcgt ccgatggcga cggaggacaa tcgcatcatc ccgatcgagc aatatggatt
     1861 gcggctcatg tcgatgggct tcctgctcga cgacgcggcg ccggccattt tgcgcggtcc
     1921 gatggtgacc cgttatacgc agcagttttt gcggcaggtc gaatggggcg aactcgatta
     1981 tctcgtactc gatctcccgc cgggcacggg cgatatccag cttacgatcg tgcagacggt
     2041 cgcgctggcc ggcgcgatca ttgtcaccac gccacaggaa gtggcgctga tcgatgcgcg
     2101 caaagccgcc gcgatgttcg agaaggtcaa cgtgccggtg ctcggcttga tcgagaatat
     2161 gagctatttc atttctcctt cggatggaaa gcgttacgac attttcggca gcggcggcgg
     2221 cgagcgtgaa gcgaaacgaa ttcgggtgcc cctgcttggt caaatcccga tcgatatcgc
     2281 gacccgcgag gccggcgacc gcgggatgcc ggtgatggcg gaagcccctg aatctccggt
     2341 cggggcagag tttgcccagg ttgcccggcg gctgcgggaa attttatcga ccacgtaaaa
     2401 gcttgccatg ccggaccaac ttcgcctaaa tagcggccgc cttggttcgt ctaatgctaa
     2461 agagagatta tcaggaaagg atttggttga agtggtggca atgaacgaga acgctggaaa
     2521 ggcttccgaa gacgagcact ttctgaaaag ttccgtgctc tacaaggagt tccttgccga
     2581 gcgcgaagag gtcttgaaac ataagtggat cgagagcgaa aaggccggat cggacatcgg
     2641 cttcgagaag gcgctgctcg attggatcgt gaagcatcgc tccaattggc gcgagcgtag
     2701 agtgcgcgag accaggccga caaaaacatc gacctgacct cgcgcgcttc ctttaaggcg
     2761 atcctgcgag gtcgcgaagg aaaatgagca aaacccgccc aactaacgaa tccgacgccg
     2821 tccgcatctt cgatgcggaa acgatgcagc gtgccttgcg gcgaatcgcg cacgaaatta
     2881 tcgagcgcaa tcgcaatctc tccgcgctcg tcctggtcgg cattccggcg cgcggcgtcg
     2941 agctggctcg ccgccttgcg gccgtcatcg aggcgatcga aaaggtgcgc gtcgcgaccg
     3001 gggtgatcga tgtctcgatg caccgcgacg acatcggaac acgccgggcg cttcccgtgg
     3061 tcaaggtctc gcaactgccg ctgccgctcg aacagcgaac ggtggtcatc gtggacgacg
     3121 tcctctttac cggccgcacc tgtcgcgcgg cgatggatgc gatcggttcc tttggccgtc
     3181 ccgcgtgcat tcaactcgcc gccctggtcg atcgcggtca ccgcgaactt ccgatccggc
     3241 ccgattttgt cggcaagaat ctacccaccg cgttcgctga gcgggtgcgg gtgcgtctgg
     3301 agaatatcga ccaccagccg gatggcgttt ggctcttgaa accggaggca aatgaatagc
     3361 gacaaggggc gctggacgcg caaggattta ctgggcctgc gggagctctc cgtcgaggag
     3421 attaaccttg tgctcgagac ggccgacgct ttcaaacaag tcggtacccg cgaagtcaag
     3481 aaagtcccgg cactccaggg gaaaaccctg atcaaccttt ttatcgagcc gagcacgcgg
     3541 acccgcactt ccttcgagct cgcggccaag cgtctcagtg ccgatatcgt taacatctcc
     3601 gcggcggctt ccagcctaac gaaaggcgaa acgctcaagg ataccgcccg ggtgctcgaa
     3661 gcgaaccacg ccgacatcat tgtcctccgg catagctcgg ctggcgcgcc gcaattcctc
     3721 gccagccgca tcgcttcgag cgtgatcaac gccggtgacg gcgcgcacga gcatcccacc
     3781 cagggcctgc tcgatctcta caccattcgc gaaaagaagg gcgcggtggc cggactccac
     3841 gtggccatca tcggcgacat ccttttcagt cgcgtggcgc gctcgaatat tttcgcgctc
     3901 ttgaaaatgg gtgcgcgcgt cacgctcgtt gggccgagca ccctcgtgcc gcgcgagttt
     3961 gaacagctcg gcgttaccgt gtcgcacaat ctcgaagacg tgctgccaac ggtggatgtg
     4021 gtgaacctgc ttcgcatcca gcatgagcga cagcggaagg aatatttccc cggcctcggc
     4081 gaatacatcc gccttttcgg cctgacgaag gcgcgcgcca gactcttgcg gccggattgc
     4141 ctgatcatgc atccggggcc gatcaatcgc ggggtcgaac tcgacagcga gctggcggac
     4201 ggcgcgcaaa gcgtcattct cgatcaggtg accaatggtc tcgcggtccg catggctgtg
     4261 ctttatctct gcggaggaat cgccacgtca tgaacaccct cattcgcaac gcaaggatca
     4321 tcgacccggc caacgctcgt gacgaggtcg gcgatctcct cattgtcgac gggcgcatcg
     4381 cttccctctc tcaactctca accttcaact ctcaacccga tgagatcgac gcagccgatc
     4441 ttgtcgttag tcctggactg atcgacatgc acgttcactt ccgtgaacct ggttttggct
     4501 ggaaggaaac gatcgaatcg ggcgcgcggg ccgcggcggc gggcggattt acgacggtcg
     4561 tctgcatgcc gaacacttcg ccggtcgccg acagtccaag cacgatcgcg tggatcaagg
     4621 accgcgccat tgaagttgct gccgtgcacg ttcttcccac cggggcgatt tccaagaatc
     4681 tcgccggcga agagctggcg ccgatcggtt cgctcgcgca ggccggcgtg gttgccatta
     4741 cggacgacgg acgctgcgtt caaaacaacg agcttatgcg ccgcgcggtc gagtatgcgc
     4801 gaatggtcgg cgtgccggtg ctcgaccact gtcaggacta caatctcgtc gggcacggcg
     4861 tcgtccacga aggttactgg agcaccctgc tcgggttgcc cggttggccg gcggcgggcg
     4921 aggaggcgat cgttgcccgc aacattctcc tcgcggaact ttgtgaccac cggattcatt
     4981 gccagcacat cagcgcggct gggagcgtcc ggctcctccg ggaagctcgg tcgcgcgggg
     5041 tcaagatcag cggcgaagtt tgcccgcatc acatctcact gacggatgaa gcgattcaaa
     5101 attttgacac cgattgtaag atgaatccgc cccttcgggc gcagcgcgat atcgacgcgc
     5161 tcctcgaagg gatcgccgat ggcacactcg aaatcctggc gagcgaccat gcaccgcacg
     5221 cgaagttcga gaaggaagtg gagtttgacg ccgcgccctt cgggatcgtc gggctcgaaa
     5281 ccgagcttgg tttgtttctc gaccttctcg ttcatcggca ccgaaccatc gcattgccgc
     5341 gcttgatcga gatgctgacc gtgaaccctg caaaattact cggcttaaaa tgcgggacgc
     5401 tgtcgttggg cgcgccggcc gatgtcacat tgatcgatcc ggatctggaa tggacggtcg
     5461 acgccggtgc cttccaatcg gcctcgcgca acaccccgtt ccacggctgg aaactgaagg
     5521 gccgcgcggt gcggacgatc gtcgggggaa agactgtctg gcagctttag ctacgacggg
     5581 tagccgccgg aaatatagtc ccgcaactgt tcgagcgttg cgctctggga ggagatcacc
     5641 tgcttcacca gatcgccgat cgagacgacg ccgcggagag agccgtcggc gaccaccggc
     5701 agatggcgaa tccgtttttc ggtcatgaaa cggaggcatt cctccaccgt ctcgcgcgga
     5761 tgggccgtcg tcagctcggt ggtcatgatt tcacgcacgt agctggtccg ggaggtcttg
     5821 cctttgagca tcactttgcg cgagtagtcg cgctccgaga cgatcccgac cagtttccca
     5881 ttctccacta cgaggagcgc gccgacattt ttctcggcca tgagggcgat cgcttcgtag
     5941 acggtcgcat cgggtgagat cgaccagatc tcgcgatttt tctggctcag aattctttcg
     6001 atggttccgc tgacgtccac gcgaatgaga atagcggccg cccgaaagcg acgcaatcaa
     6061 ttaacacgcg cggtgcgcga actttcgggc gatcatcgcc tcgagttttg cgacgagcac
     6121 cgggtcgggc aggcgctcga gcacttcgtt gaggaagccg ccgacgagca attcgcgcgc
     6181 gacgggcgcg gggatgccgc gagcgaggag gtaaaacatt tcgtcgtcgt cgagctggcc
     6241 ggaggtggca ccgtgactgc atttgacgtt gtcggccatg atctcgaggc cgggcatgga
     6301 gttcgcctcc gcgtcgtcgc tcaacaggag attgcgcact ttttggtagg cgtcggtgaa
     6361 atgcgcgtgg ggctcgaccc ggatcaggcc gccaaaggta ttgcgcgacc ggccgttgag
     6421 cgcgttcttg aacaagagat cgctgtaagt gtgcggcgaa gcgtgatcct gcagggtgcg
     6481 agcgtcgaat tcctgggctt catttccgat cgaaaccgcc agcatgtcgc tgtgcgcgcc
     6541 ctcgccggcg agacggctca gactttcgag acgtgaatat tttccgccga gaccgagatg
     6601 aagattcaac gccctcgcat cgcgcgcgac ggtcgtggcg ttgatctgaa tgctcaacgt
     6661 cttgtcgctc caatcctgcg cgcagatgta ggtgaccttc gcgccggggc cgacgatcag
     6721 atcattcaca ccgcaggcga agccggcgcg ctgtgggtcg gtcgagcgga agtgctcgac
     6781 cagcgttatc ttcgagaggt cttcggctac gacgagcgtg tgcgggaaaa tcgcggcgtt
     6841 ctcggtgtgc agccagtgaa aggtttcgag cggcagttcc acttccaccc cgcgcggcac
     6901 ataaagaaag gttcccgacc taacgaacgc ctcatggagc gcggaaaatt tggcggagcc
     6961 gagcgcggcc ggctgactca tgaagtgacg gcggaagagt tcttcgtgct cgatcatcgc
     7021 tcgctcgagc ggctggaaga tgacgccggc tttgcgcaga ttttccgaaa ggacgtcgcg
     7081 ttcgaggaaa tgatcgtcgg cgaaaaccaa acgggcggag gtttcatcga gctcgcccga
     7141 gcgttcgagg atttcgtggc tatctgcttc cgacaggcgc ccggcggtag aatagggcga
     7201 aagatcgagc aggccgacgt tggaaaagcg ccaggcttgg tcggtccgca cgggcatcgg
     7261 caggctctgg aattgcgtcc aggccgcctg ttgctgatcg ccaaaccagg cgggcacctg
     7321 gtcgtcaccg tgcctctgga ttccctcaag aattccggcc atcttgggcg cgactgcgga
     7381 ggttttttcg agaacggctg acgggttgct catagcgggc tgagacgcga acggcggtcc
     7441 aagaccgccg tcaaactgcg tccgtttctg cgggtgaaac ctagccgacg gagccttcca
     7501 tctcaagttc gatcaggcgc tttagctcaa ccgagtattc catcggaaat tgccggacga
     7561 gatcgttgac gaatccgttc acactcaagc tcatcgcctg cgccttggtc aggccgcgct
     7621 gttgcatgta gaagatctgc tcggcgctga cctggctgac actcgcctcg tgctgcaccg
     7681 cattgccggt gccgcgaacg gtgatggcgg gataggtgtc ggtgcggctg ttcgcgttga
     7741 tcaggagcgc gtcgcactcg gtgttattct tgcagttctt gagatgcttc ggcacgtgaa
     7801 ccaagccgcg gtaggtggcc cggccggtgc cgatcgagat gctcttggaa atgatgttgc
     7861 tcgtcgtttc gtcggcggcg tgcaccatct tcgcaccggt gtcctggtgc tgaccgtcgc
     7921 ccgcgagcgc gatcgaaatc acttcgccgc gcgccttacg gcccttcatc acgacgccag
     7981 ggtacttcat cgtgaggcgg gaaccgatgt tgcagtcaat ccatttcact tccgcctcct
     8041 cgtgggcgag gccgcgtttg gtgacgaggt tgaaaacgtt gttcgaccag ttctggacgg
     8101 tgatgtactg gagtttcgcg cctttcaggg cgaccagctc gacgaccgcg ctgtgtaacg
     8161 tggcagtatt gaatttcggc gcggtgcagc cctccatgta ggtcagctcg gcgccttcat
     8221 cgacgatgat gagggtgcgc tcgaattgcc caaaattctc ggcgttaatc cggaaatagg
     8281 cctggagcgg atgcttcacc ttcacgcctg gaggcacgta gatgaacgag ccgccactga
     8341 aaacggcgct gttcaacgcg ctgaatttgt tgtccccggt cggaatcact ttgccgaacc
     8401 atttccggaa aatctccggg tgatgtttca aaccatcggc gctcccgacg aaaatcactc
     8461 cctgttcgcc gaccgctttc ttgatgttgg aatagaccgc ttcgctatcg aattgcgctt
     8521 ctaccccggc gaggaatttc cgttcctgct ccggaatgcc gagccgctcg aaggtgcgct
     8581 ttacatcgtc gggcacatcc tcccaactgc gttttggctg ggtaccgccg ctgagatagt
     8641 agcggatatt atcgaacacg atcgcttcca gatccttgct cgcccaatgc gtcggcattg
     8701 gtttgctcag aaaggtgcgg agcgcgttga ggcgaaattc ccgcacccag tcgggatcct
     8761 gtttcacatc cgagatgtag tggatcgtct tttcgctcaa accgatgccg gcatcgtggg
     8821 tgtgcgtctc cgggtaagcg aaattaccca ggttccgttc gatatcgagc gtcttgtttt
     8881 ccttaaccat ttcagtctcc ttcttaggcg agcgcgggct cggtgatgtc ctcccgcacc
     8941 caatcataac ccttttcttc caactgcagg gccaaatctt tgtctccgct gagctcgatc
     9001 cggccgcgca ccatgacgtg cacgtgatcg ggtacgatgt aattcaaaag ccgctggtaa
     9061 tgcgtgatca aaagcacgcc gagattcggc ccgcggagcg agttgacccc atgcgcgaca
     9121 actctgagcg cgtcgatgtc gagcccgctg tcggtctcat cgagtaccgc gtacttcgga
     9181 ttgagcatcg ccatttgcag aatctcgttg cgctttttct cgccgccgga aaagccttcg
     9241 ttcaccgcgc gcgaagtaaa agcgcgatcc atgttgagca gatccatttt ctggtaaagc
     9301 tgcgcgtagt aatcggtcgc ctccagttcc tcgccctccg gcaaacgtgc ctggactgcg
     9361 gcccggagga aattggcgat cgtcacgccc gggatttcgc tcgggtattg gaaggccatg
     9421 aaaagcccac ggcgcgcccg ctcatccggc tcgagctcga gcacgttctc gccgtccatt
     9481 agcaccgaac ccgaagtcac gacgtaatcc gggtgcccgg ccagcacctt cgcgagcgtg
     9541 ctcttgccgg agccatttgg ccccatgacg gcgtgcactt ctcccttggg aatggcaagg
     9601 ctgagaccgc gaatgatctc ctgatcggcg atcgaaacat gtaaatcctg aatgaccaat
     9661 tgatccataa tttgcgaagt ggaagatacg ccggaactcc cgtttttgcg aacgcaattc
     9721 atccctccgt cgttgctctc gacaccggcc ggagttgtgg cagccgtcaa tctctggtta
     9781 aagtccggtt tgaatcatgg acgaccctca cgctgctgat caagaccgac aaatccgggc
     9841 ttttgtgctc tgcggcgggc tcggtactcg actccgttcg gtcttgagcg atcgaccaaa
     9901 gtcgatggcg cccgttgctg gcgttccttt tctccaactg cttctcgagg accttaggtc
     9961 ccagggcatc ctcgaaatca tcctcggaac cggttacatg tcggagcagg tcgaggcttt
    10021 tttcccgcag ggcgagaaca tcggcctgca cgttcgctac tctcgagaga acaagcctct
    10081 cgggaccggc ggagccctta aactggctga atcgctcttg agcgatccgg tcgtggtcct
    10141 caatggagat agctatgtgg agtggagcct cgcggcgacg gtggagctct tccgcaaaaa
    10201 agacgcggcc atcgtcatcg tcctgcaaac ggtcgcggat gtgagtcgtt atggaagcgt
    10261 gacgatcgag gcggatggct gcgtgaccga gtttgtcgag aaaggaactc ggaccggccc
    10321 gggcttgatc aatgccgggg tctacctgtt gcggagggaa atcgtca
//
LOCUS       DATMNS010000070        10291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3626446, whole genome shotgun sequence.
ACCESSION   DATMNS010000070 DATMNS010000000
VERSION     DATMNS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3626446"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1761)
                     /locus_tag="VNW28_02665"
     CDS             complement(163..1761)
                     /locus_tag="VNW28_02665"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXA08855.1"
                     /translation="MKYIFVTGGVVSSLGKGLAAASLGTLLELRGQRVLLQKFDPYLN
                     VDPGTMNPFQHGEVYVLNDGAETDLDLGHYERFTNCVLSRNNNLTSGQVYESVILKER
                     RGDYLGNTVQVIPHVTDEIKARIRQVADPAKVDVVITEIGGTVGDIEGLPFLEAIRQF
                     ILEVGAQNVVNMHVTLVPYIRAAGELKTKPTQQSIAKLREIGLQPQVLICRTERPLDA
                     DVRQKLSLFGNVPRNAVIEARDVPHTIYEMPLMLHEEGLDELVCQLLHVDAPQPDLGD
                     WRKFVSRVVNPKKQVKIAVVGKYIDLQDAYKSIYESLTHAAASHDCGIDLRLIDAESL
                     ENGVDGQLADVAGVLIPGGFGERGVEGKIRAAQFARENNVPYLGLCLGMQVATIEFAR
                     NVCGIAAANSSEFDQESAEPVICLLDEQRKIRKKGGSMRLGTWPTRIVHGTLAERIYK
                     HDEVLERHRHRYEFNMKYRAQMEEKGFLISGTSPDGALAELIELRDHPFFLACQYHPE
                     FQSKPNKPHPLFSGFIRACLAHQG"
     gene            complement(1758..2522)
                     /gene="kdsB"
                     /locus_tag="VNW28_02670"
     CDS             complement(1758..2522)
                     /gene="kdsB"
                     /locus_tag="VNW28_02670"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HXA08856.1"
                     /translation="MTKTVGIIPARWGSTRFPGKPLHRIAGRPLLQHVWERCRRARAL
                     DSVIIATDDMRIAEAAFAWGAEVSLTSPVHASGTDRIAAVAAKLHGVSHIINIQGDEP
                     LVAPSLINQLARKMQHDPQIEMITAVHPFENPADAQSPHQVKAVLDRQGRALYFSRSP
                     IPFHREASATISYYRHQGIYGYRRDLLLRFVRWKPAALEKAEALEQLRALENGVIIHV
                     VVTSSGSPGVDTPDDARAIGRQLLAAARRPRQVRSR"
     gene            complement(2733..3302)
                     /locus_tag="VNW28_02675"
     CDS             complement(2733..3302)
                     /locus_tag="VNW28_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXA08857.1"
                     /translation="MTTAPNEPAPAVFLDRDGTLMREVNYCADPQQVEIFPGVPAALQ
                     RLKAAAYKLIVISNQAGIGRGYFTEEQYRAVEAEVARAVLPATFDGVYFCPDRPDLAT
                     NRRKPAPGMVFEAQRDHDIDLARSFFIGDKAIDIACGRNAGVRTILVKTGYGAHETHS
                     APHWIAEDMSAAVDIILNLNETSGGDSPG"
     gene            complement(3286..4296)
                     /locus_tag="VNW28_02680"
     CDS             complement(3286..4296)
                     /locus_tag="VNW28_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXA08858.1"
                     /translation="MKLERLQEILARAAGKRLAVAGDLMLDEFVWGKVGRISPEAPVP
                     VVEVTGESFYPGGAANVARNLREFTHRVAVIGTVGTDGGGRQLLDLLRAQEIDTGLVV
                     TDPAFRTIVKTRIVARHQQVVRVDREQIREPAAPQVAQVVAGLRKILPELDAFIFEDY
                     GKGFLSADLVRQISRDLSAAGKITTADPNPHRVIDWGGVTAVKPNRSEALHAAGFPAT
                     DPVDPPLEDAALLAAGAKLLTAWDTQMLLITLGEQGMMLLERNKTPHHIPTRARQVFD
                     VSGAGDTAIALFTLALCCDASPVEAAQIANHASAVVVAKLGTATVTPAELVASFEDDN
                     GA"
     gene            4486..5259
                     /locus_tag="VNW28_02685"
     CDS             4486..5259
                     /locus_tag="VNW28_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08859.1"
                     /translation="MAGWVLAGLRSDSMAAEPARRIDVKRLSKNIDDVVVPLPNEIFG
                     ALNKLDSVNWKEFVRTNKGTNFTERPRIALLLGTVIADGFIAVQAEDAPSVKEIGQRV
                     VNLAKGIGVGSSIVPHAKAITEAADHRNWPAVRQELDRTQNSVQQAMNEVHDEKLSQL
                     VSLGGWLRGTEVLTAVVNRHFSADGAELLHQPDLLDYFQRKLNGMPEFDLPLLGKIKS
                     SLVEVKPLIDVDSPISPASVKKINEITTSLDEGIITRSP"
     gene            5266..5718
                     /locus_tag="VNW28_02690"
     CDS             5266..5718
                     /locus_tag="VNW28_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08860.1"
                     /translation="MRPPSPTARRTALFLGALLALQVAASRASVDDAHSFALESAEPY
                     VKQGYQVREDYWGGDLGAGEKKAVRQQLFKGNDYWFWMGTEVERAKVSVHVYDADGRL
                     SEQPDSWQKGHFAAAHLIPKTTGSYFIIVAVESSPEERTHWALVYGFK"
     gene            5846..6829
                     /locus_tag="VNW28_02695"
     CDS             5846..6829
                     /locus_tag="VNW28_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HXA08861.1"
                     /translation="MTETRTLQFENARAVQSLYANDLKLIKNLEESLGVKVTTREGWI
                     KMEGDAAQVAKAERVFTQLESARQRGVDIQRHEFTYALNSVNEAREDKLDDLVAIKIV
                     TSARKPPIVARTLGQRNYLEAIQKQDVVFGIGPAGTGKTYLAVAMAVSALKKEQVDRI
                     VLTRPAVEAGEALGFLPGDLQQKITPYLRPLYDALRDMLEPEEIERYVARETIEIAPL
                     AYMRGRTLSNAFVILDEAQNTTTEQMFMLLTRIGPRSKCVVTGDGTQIDLPLNKRSGL
                     LEALQALKNVPGIGFVYFSDKDVVRHELVRAIINAYQQHRSPANTGGAARK"
     gene            6866..8473
                     /locus_tag="VNW28_02700"
     CDS             6866..8473
                     /locus_tag="VNW28_02700"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXA08862.1"
                     /translation="MKRGLASGKTRRRRPSNEMLRSLEHALWMRVTIFAAFVAGLALL
                     VFSGQQQEPTRNFVFALLLLATAVAQLWVNQPKTFGRNSRVFLVFGIIYLQLGLTKLL
                     FALCNSGVVASLKPEAAWLIAPYAFAPLVLCVLLGRNHGLYGAVFVSLWSSILVGKID
                     APILVIGLISGFTAVLLTQQVRRRSRLIRAGLFVGLAIWLLSLTFGLIGPINLFNPVA
                     TDWSMIGIQSAFAIGNGLVTAMVVGGALPILEQVFRITTDISWLEASDLNHPLLRRMT
                     IESPGTYHHSLVVANLAESAAEAIGANATLCRVCSYFHDVGKLVKPDYFTENMNFERN
                     PHDDLAPTMSALIIIAHVKEGVDLALKHGLNQQVIDVIQQHHGTSLVFYFYNRALQQH
                     EDARAGGKIMKMREEDIPEVSEESFRYSGPKPQTRESAIISLADMVESASRSLEKPTP
                     QKIEQLVTELIDQRLADHQLDECELTLRELRTIAERFRFTLMNMLHTRIAYPKEGKGS
                     TTREDGARPDVMAATNRPASAPPVSAA"
     gene            8477..9244
                     /locus_tag="VNW28_02705"
     CDS             8477..9244
                     /locus_tag="VNW28_02705"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HXA08863.1"
                     /translation="MEDFASERVREFGAQVAIVLGSGLNSLVQDPPRERVIPYREIGG
                     LPGSAVKGHLSRFVLDEISGTRVVFSQGRAHLYEGYSARDVTAGVRFLARAGIKKLVL
                     TNAAGSVNPDFEPGSWMMITDHLNLTGTTPLLGESDFVDLTDAYSPDWRGRLAAAARA
                     MGLKLHEGVYAGMLGPQYETPAEVRMLRLLGTDAIGMSTVCETIQARALGLEVAAFSC
                     LTNWAAGLNPGPLSHREVLAAGEASGTVFMRLLGAAL"
     gene            complement(9380..9697)
                     /locus_tag="VNW28_02710"
     CDS             complement(9380..9697)
                     /locus_tag="VNW28_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08864.1"
                     /translation="MIKTTFALLATVVLATSSAFAGGACCAGMSADGKMDCSKAYAQM
                     NLTADQKTKLDAAQADCQKAGCTKESMDKFMHSAKHILSAKQYAELKSQCARMQPAEK
                     TKS"
     gene            complement(9757..>10291)
                     /locus_tag="VNW28_02715"
     CDS             complement(9757..>10291)
                     /locus_tag="VNW28_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXA08865.1"
                     /translation="IEKAHPDVFNVLLQVLDDGRITDGQGRTVDFKNTVIIMTSNIGS
                     QFITEETSGEERERRVLDALRQHFRPEFLNRVDEIIIFDRLNEEDLKKIVEIQLRRLT
                     KRLENQKIMLELTDAAKARIAREGYDPVYGARPLKRAIQKEILDPLSLQILEGKFHEG
                     QTIKVDERAGKLIFVAA"
BASE COUNT         1930 a         3126 c         3187 g         2048 t
ORIGIN      
        1 cgtttgcggc gactcgccgc aaacattggg gcgggattag cttcacggct tcgccggcgg
       61 ggattggacc aaagctccgt tcggtgagtc gcctcacgga gcacgcgagt cgcgtgcgct
      121 ccccggaact tgggtcgccg cgccgtctat cctcgttagg cactagccct ggtgcgcgag
      181 gcaggcgcgg atgaagccgc tgaaaagcgg gtgcggcttg ttcggtttgc tctggaattc
      241 cgggtgatac tggcaggcga ggaagaacgg gtgatcgcgc aattcgatca attcggcgag
      301 cgctccgtcg ggtgacgtgc cgctgatgag aaaacctttt tcctccatct gggcgcggta
      361 tttcatgttg aactcgtagc gatgccggtg gcgctcgagc acttcgtcat gcttgtagat
      421 tctttccgcg agcgtcccat gcacgatcct ggtcggccag gtcccgagcc gcatgctgcc
      481 gccttttttt cgaattttcc gctgctcatc gagcaggcag atgaccggct cggctgattc
      541 ctggtcgaac tcgctgctgt tggcggcggc gatgccgcag acattgcgcg cgaactcgat
      601 cgtcgcgacc tgcattccga gacatagccc gagatagggc acgttatttt cgcgggcgaa
      661 ctgggcggcg cgaatttttc cctccacccc gcgctcgcca aagcctccgg gaataagcac
      721 gccggccaca tcggcaagct gaccgtcgac gccgttctcg agcgattcgg catcgatcag
      781 gcgcagatcg atcccgcaat cgtggctcgc ggccgcgtgc gtgagcgact cgtaaatgct
      841 tttgtaggcg tcctgcaagt cgatgtattt gccgacgacc gcgatcttga cctgcttctt
      901 cgggttgacc acccgactaa cgaacttgcg ccagtcgccg agatcaggtt gcggcgcgtc
      961 gacgtgcagc aattggcaga cgagctcgtc gagcccttct tcatgcagca tgagcggcat
     1021 ctcgtaaatc gtgtgcggca catcgcgcgc ctcgatcacc gcgttgcgcg ggacattgcc
     1081 gaagagggaa agcttttgcc tgacgtcggc gtcgagcggc cgttccgtgc ggcaaatcaa
     1141 gacctgcggt tgaaggccga tctcgcgcaa tttcgcgatg ctttgctggg tcggtttggt
     1201 tttgagttcg cccgccgccc ggatgtaagg cacgagcgtg acgtgcatgt tgactacgtt
     1261 ctgcgcgccg acctcgagaa tgaactggcg gatggcttcg agaaagggca gcccttcaat
     1321 gtcgccgacc gtcccgccga tctcggtgat aacgacatcg acctttgcgg gatcggcgac
     1381 ctgccgaatg cgcgccttga tctcgtcggt gacgtgcggg atcacctgca cggtgttgcc
     1441 gagataatcg ccgcggcgct ccttcaggat gacactttca tagacctggc cgctggtcag
     1501 attgttgttc cgcgagagaa cgcaattggt gaaacgctcg taatggccga ggtcgagatc
     1561 ggtctcggcg ccgtcgttca ggacgtaaac ctcgccgtgt tggaacgggt tcatcgtgcc
     1621 gggatcgaca ttcaggtaag ggtcgaactt ttgcaggaga acgcgctggc cgcgcagctc
     1681 gaggagcgtg ccgagggaag cggccgcgag gcctttgcct aacgaactaa ccacacctcc
     1741 agtaacaaaa atatatttca tcgggaacga acctggcgcg gtcgtcgcgc ggcggcgagc
     1801 aactgacgcc cgatcgcccg ggcgtcgtcc ggggtgtcca ctccgggcga gccgctgctg
     1861 gtcacgacca cgtgaatgat tacgccattc tccagcgcgc gcaattgttc gagcgcttcc
     1921 gccttttcca gcgccgccgg tttccagcgg acaaagcgga ggagaaggtc gcggcggtaa
     1981 ccgtagatcc cctgatgccg gtaataggag atcgtcgcgc tcgcctcgcg gtgaaacggg
     2041 atcgggctcc gcgaaaaata gagggcgcgc ccttggcgat cgaggacggc cttgacctgg
     2101 tgcggcgact gcgcgtcggc gggattctca aacgggtgca ccgcggtgat catctcgatc
     2161 tgcgggtcgt gctgcatttt acgcgcgagt tggttgatca agcttggagc aacaagcggc
     2221 tcgtcgccct ggatgttgat gatgtgcgag acgccgtgca atttcgccgc caccgccgcg
     2281 atccggtcgg tcccgctggc atggaccgga ctcgttaggc tgacctccgc gccccaggcg
     2341 aaagcggctt ccgcaatgcg catgtcgtcg gtcgcgatga tgacggagtc gagcgcgcgc
     2401 gcccgccggc aacgttccca gacatgttgc agcaagggtc ttcccgcgat gcggtgcagg
     2461 ggcttgcccg ggaagcgggt cgaaccccag cgggcgggga taatgccgac ggtttttgtc
     2521 atgaatgctc gcagcctaac agcggctgga agcgccttgc aacgttgctg gtctctccgg
     2581 gtagcgcacg cgtctcgcgt gctggtcgcg gtgtctcgcc gcgacgaact ttcgcaatga
     2641 gttggcaagg tggcgggatc gatcactttc ttgccgcaga ttgggaagtt cgcgacggcg
     2701 gaacgccgtc gccagcacgc gagacgcgtg cgctacccag gggaatctcc acccgaagtt
     2761 tcattgagat tcaaaatgat gtcgacggcg gccgacatat cttccgcgat ccaatgcggc
     2821 gcgctgtggg tttcgtgcgc accgtaaccg gttttcacca aaatcgtccg gacaccggcg
     2881 ttgcgcccgc acgcgatgtc gatcgccttg tcgccgatga aaaaagagcg ggcgagatcg
     2941 atgtcgtggt cgcgttgtgc ctcaaaaacc atgcccggcg cgggcttgcg ccgattggtc
     3001 gcgaggtccg gccggtcggg gcaaaaataa actccgtcaa aagtggccgg gagaacggcc
     3061 cgcgccactt ccgcttcgac cgcgcgatat tgttcttcgg taaaatagcc gcgtccgatg
     3121 ccggcctgat tggagatgac gatcaatttg taggcggccg ctttcagacg ctggagcgca
     3181 gcgggcaccc ccggaaagat ttctacctgc tgcgggtcgg cgcagtaatt cacctcgcgc
     3241 attaacgttc catcgcggtc gagaaagacg gcgggcgccg gttcgttagg cgccgttgtc
     3301 atcctcgaag ctggcaacca actccgccgg ggtgaccgtg gccgttccca gttttgcgac
     3361 gaccacggcg ctggcgtgat tggcgatctg cgccgcctcg acgggcgagg catcgcaaca
     3421 gagggcgaga gtaaagagcg cgatcgcggt gtcgccggcg cccgaaacgt cgaagacctg
     3481 acgcgctctg gtcgggatgt ggtgcggggt cttgttgcgc tcgagcagca tcatgccctg
     3541 ttcgcccagg gtaatcagga gcatctgcgt gtcccaggcg gtcagcaatt tcgccccggc
     3601 cgcgagtaac gcggcgtcct ccagcggcgg atcgaccgga tccgtcgcgg ggaagcccgc
     3661 ggcgtgcagc gcttcgctcc ggtttggctt caccgccgtg acgccgcccc agtcgattac
     3721 ccggtgcggg ttcggatccg ccgtcgtaat tttcccggcg gcgctgaggt cgcgggaaat
     3781 ctgcctaacg agatcggcgc tgagaaaacc cttgccataa tcctcgaaaa tgaaggcgtc
     3841 tagttccggc aggatttttc gcaggccggc cacgacctgg gcgacctgcg gagccgccgg
     3901 ctcacgaatc tgttcccgat cgacgcgaac gacctgctga tggcgcgcga cgatgcgtgt
     3961 tttcacgatg gtgcgaaaag cgggatcggt cacgacgagg ccggtgtcga tttcttgcgc
     4021 gcgtaacaaa tccagcaatt ggcggccgcc gccatccgtc ccgaccgtcc cgatgaccgc
     4081 aaccctgtgg gtgaattcgc gcaggttgcg ggcgacgttg gccgcgcccc cggggtaaaa
     4141 tgattcgccg gtgacctcga ccacgggcac gggcgcctcc ggcgagatgc ggccgacttt
     4201 tccccagacg aattcgtcga gcatgagatc gccggcgacc gcgagccgct taccggcagc
     4261 ccgggccaga atctcctgca gacgctccag tttcataaga agagaagcgg actactccgc
     4321 gcccgatgcc accgccgaaa tgggttgctc cgatttaata acactggatc gtatcgcgat
     4381 ttgtgccgcc gcgcggttct gcataaagtg ccccactaat cctgaatacc ttctcgaaat
     4441 caatagtcga agctgcggat gagatctttc tttaaaatac ttcttttggc gggctgggtc
     4501 ctggctggtc tgcgctccga ctccatggcg gcggaaccgg cgcggcggat cgatgtcaaa
     4561 cggctctcga aaaacatcga cgacgtggtc gttccgctgc ccaacgaaat cttcggcgcg
     4621 ctcaacaagc ttgatagcgt gaactggaag gaattcgtgc gcacgaataa aggcacgaat
     4681 ttcaccgagc gcccgcggat cgccctcctt ctcgggacgg tcatcgccga cggtttcatc
     4741 gcggtgcagg cggaggacgc tccgagtgtg aaggagatcg ggcagcgcgt cgttaatctg
     4801 gcgaagggca ttggggtggg aagctcgatt gtgccgcacg cgaaggcgat cacggaggcg
     4861 gccgaccacc gcaattggcc ggcggtgcgg caggaactcg accgcaccca gaacagcgtg
     4921 caacaggcga tgaacgaagt gcacgacgag aagctgtcgc aactcgttag tctcggtggc
     4981 tggctgcgcg gcaccgaggt tttgacggcg gtcgtcaacc ggcatttttc ggcggacggc
     5041 gcggagctgt tgcaccagcc cgacctgctc gactatttcc aacgcaagct caatggaatg
     5101 ccggaattcg accttccgct cctgggcaaa atcaaaagct cgcttgtcga agtaaagccc
     5161 ttgatcgatg tcgatagccc gatctcgccg gcgtcggtga aaaagatcaa cgagatcacg
     5221 accagcctcg acgagggaat catcacgcga agtccctgac ccccgatgcg tccgccttct
     5281 ccaaccgccc gtcgcaccgc tcttttcctt ggcgcgttgc tcgccctgca ggtcgccgcg
     5341 tcgcgcgcct cggtcgatga tgcgcattcc ttcgccctcg agtcggccga gccatacgtc
     5401 aagcaaggct atcaggtgcg ggaagattat tggggcggcg acctcggggc aggggagaaa
     5461 aaagccgtcc ggcagcaact tttcaaaggc aacgattact ggttctggat gggaaccgaa
     5521 gtggagcggg cgaaggtttc cgtccatgtt tatgatgccg acgggcggct gagcgagcag
     5581 cccgatagct ggcagaaagg gcacttcgcc gcggcgcacc tcatcccgaa gaccaccggc
     5641 agctatttca ttatcgtggc cgtggagagt tcacccgaag agcgcacgca ctgggcactg
     5701 gtttacggct tcaagtaaac ctaacacgcg aggaagcgac agcgcgcctc gcttcaggtc
     5761 gtcgcgaggg ttggctgacc ctgaaggttt gacactcttc ccggcaaagc cgtatcctcg
     5821 cggctaatga tgttccagcc gcgagatgac tgaaacgcgc acgctgcagt ttgagaatgc
     5881 gcgggccgtc cagtcgctct acgccaatga cctgaagttg atcaaaaatc tcgaggaatc
     5941 gctcggtgtg aaagtcacga ctcgcgaagg ctggatcaag atggaaggcg acgcggcgca
     6001 ggtcgcgaaa gccgagcgcg tcttcaccca actggaaagc gcgcggcaac gcggcgtcga
     6061 tatccagcgg cacgaattta cctacgccct gaactcggtg aacgaagccc gcgaagataa
     6121 gctggacgat ctcgtcgcga ttaagatcgt gacctcggcg cgcaagccgc cgatcgtcgc
     6181 gcgcactctg ggccagcgca attatctcga ggcaatccaa aaacaggacg tcgttttcgg
     6241 catcggaccg gcgggaactg gcaagaccta cctcgcggtg gcgatggccg tttccgccct
     6301 gaaaaaggaa caggtcgacc gcattgtcct gacgcgtccg gcggtcgagg cgggcgaagc
     6361 gctcggattt ttgccaggtg atttgcagca aaaaatcacg ccgtacctgc gtccgcttta
     6421 cgacgcgctg cgcgacatgc tcgagccgga ggagatcgag cgctatgtgg cgcgcgagac
     6481 gatcgagatc gcgccgctcg cctacatgcg ggggcgcacc ctgagcaacg cgttcgtcat
     6541 tctcgatgaa gcgcaaaaca ccacgaccga acaaatgttc atgctcctga cgcggatcgg
     6601 gccgcgctcg aaatgcgtcg tgaccggcga cggcacccag attgatctgc ccttgaataa
     6661 acgctcagga ttgctcgagg ctctccaggc gctgaaaaac gtgcccggga tcggcttcgt
     6721 ttattttagc gacaaagatg tcgttaggca cgagcttgtc cgcgcgatca tcaacgccta
     6781 ccagcagcac cgttcgccgg cgaataccgg cggcgcagcc cgaaagtagc gcctcatgtt
     6841 tgagttcctg cacagaaccc gcctcgtgaa acgagggctc gcttcgggca agacccggcg
     6901 gcgtcgcccc tcgaacgaaa tgctgcgcag tctggagcat gccctctgga tgagggtcac
     6961 gattttcgcc gccttcgtcg ccgggctggc gctcctcgtt ttcagcggcc agcagcagga
     7021 accgacgcgt aattttgtct tcgcgctcct cttgctcgcc accgccgtgg cgcagctttg
     7081 ggtcaatcag ccaaaaacct tcggacgaaa ctcccgcgtc ttcctcgtct tcggaatcat
     7141 ttatttgcag ctcggcctga cgaagctcct tttcgcgctc tgcaacagcg gcgtggtggc
     7201 ttctttgaaa ccggaggccg cctggctgat cgctccctac gcctttgctc cgcttgtcct
     7261 ctgcgtcttg ctcggccgta accatggcct ctacggcgct gtcttcgtta gtctttggag
     7321 cagcattctc gtcggcaaaa tcgacgcgcc gattctggtc attggcctga tcagcggctt
     7381 caccgccgtc ctgctcaccc agcaggtgcg ccgccgcagc cggctcatcc gggccggcct
     7441 tttcgttggt ctcgccatct ggcttctctc gctcactttc ggattgatcg gtccgatcaa
     7501 cctcttcaat ccggtggcga ccgattggtc gatgatcgga atccagagcg cgttcgccat
     7561 cggcaacggc ctcgtgacgg caatggtggt cggcggcgcc ctgccgatcc tcgagcaggt
     7621 cttccgcatc acgaccgata tttcctggct cgaagcctcc gatttgaatc acccgctctt
     7681 gcgccggatg acgatcgaat cgcccggcac ctatcatcac agcctggtcg tcgcgaatct
     7741 cgccgaatca gcggccgaag cgattggcgc gaacgccacc ctttgccgtg tctgctccta
     7801 tttccacgac gtcgggaagc tggtgaagcc ggattatttc accgagaaca tgaacttcga
     7861 acgcaacccg cacgacgacc tggccccgac gatgagcgcg cttattatta tcgcgcacgt
     7921 caaggaaggc gtggatctgg cgctcaagca cgggcttaat cagcaggtga tcgacgttat
     7981 tcagcagcat cacggcacat cgttagtctt ctacttctac aaccgcgcgc tgcagcagca
     8041 cgaggacgcg cgggccggcg ggaagatcat gaagatgcgc gaggaggaca ttcccgaggt
     8101 gtcggaggaa agctttcgct atagcggccc gaaaccgcag acccgcgaga gcgcgatcat
     8161 cagcctcgcc gacatggtgg aaagcgcgtc gcgctcgctg gaaaaaccaa cgccccaaaa
     8221 aatcgagcaa ctggtcaccg agctgatcga tcaacgcctc gccgaccatc aactggacga
     8281 atgcgagctg accctgcggg aattgagaac gatcgcggag cgcttccgct tcaccttgat
     8341 gaacatgctg cacacgcgga tcgcctatcc aaaggaagga aaaggctcga cgacccgcga
     8401 ggacggcgcg cgtcccgatg tgatggcggc aacgaaccga ccagcctccg cccccccggt
     8461 ctcggccgcc tgacgagtgg aggatttcgc gagcgagcgg gtgcgcgaat ttggagcgca
     8521 ggttgcgatt gttctcggct cggggctgaa ctcgttagtc caggacccgc cccgcgagcg
     8581 cgtcatccct tatcgcgaga ttggcggcct gccgggctcg gcggtaaaag gtcatctcag
     8641 tcgcttcgtc ctcgacgaga taagcggcac gcgggtcgtc ttctcccagg gacgagccca
     8701 tctttacgaa ggctacagcg cacgcgacgt cacggccgga gtgcgctttc tcgcgcgcgc
     8761 gggaatcaaa aaactcgttc tgacgaacgc cgcggggtcg gtcaatccgg atttcgagcc
     8821 gggctcgtgg atgatgatta ccgaccatct taacctgact gggaccacgc cattgttagg
     8881 cgagagcgac ttcgtcgact tgacggatgc ttactcgccg gattggcgcg gacgtctcgc
     8941 ggccgcggcc cgggcgatgg gcctcaagct ccacgaaggg gtctatgccg ggatgctcgg
     9001 tccgcaatac gagacgccgg ccgaagtgcg gatgctgcgg ctcctgggga cggacgcgat
     9061 cgggatgtca acggtctgcg aaacgatcca ggcgcgcgct ctcgggctgg aggtcgccgc
     9121 cttttcctgc ctaacgaatt gggccgccgg gttgaacccg ggaccgctct cgcatcgtga
     9181 ggtgctggct gcgggcgaag cgagtggcac cgtgttcatg cgcctgctgg gcgccgcgct
     9241 ctagggcacc gtatcagatg tttcgtgagt gaggatgata tacgtgagtc acgtatatca
     9301 tcaaatgcac ggttggagcg cgatgtcatc gcgacgacag agcgtcgcgc tccacgcacg
     9361 tttcaagtga tacagggcac tagctcttcg tcttttccgc cggctgcatc cgtgcgcact
     9421 gggatttgag ctccgcatac tgcttggcgg agaggatgtg tttcgcggag tgcatgaact
     9481 tgtccatgct ttccttggtg caacccgcct tttggcaatc cgcctgggcc gcatcgagct
     9541 ttgtcttctg atcggcggtc aggttcatct gggcgtaggc tttcgagcaa tccattttcc
     9601 cgtccgctga cataccggcg cagcaggcgc ctcccgcgaa agcggagctg gtggcgagaa
     9661 cgacggtggc gagcagagcg aaagttgttt ttatcatacg caagagatcg cacgccggtg
     9721 cgaaaattca accaaccggg tggtcagtcg gacgctttac gcggccacga aaatcagctt
     9781 ccctgcccgc tcgtcgactt tgatcgtttg cccttcgtga aattttccct ccagaatctg
     9841 gaggctgagc ggatcaagaa tctccttctg gatcgcgcgc ttcagcggcc gcgctccgta
     9901 aaccggatcg tagccttcgc gcgcaatccg cgctttggcg gcgtcggtca gctcgagcat
     9961 gatcttttgg ttctcgagcc gtttcgttag gcgacgcagt tggatctcga cgatcttctt
    10021 caggtcttcc tcattcagcc ggtcgaagat gatgatctca tcaacgcgat ttaaaaactc
    10081 cggccggaaa tgttgccgca gcgcatccag cacccgccgc tcgcgctctt cgcccgaggt
    10141 ttcctcggtg atgaattgcg acccgatgtt cgaggtcatg atgatgaccg tgtttttgaa
    10201 atcgaccgtc cgtccctggc cgtcggtgat ccggccgtcg tccagcactt gcaggaggac
    10261 attgaaaaca tccgggtgcg ccttctcgat t
//
LOCUS       DATMNS010000071         5669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5101159, whole genome shotgun sequence.
ACCESSION   DATMNS010000071 DATMNS010000000
VERSION     DATMNS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5669
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5101159"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VNW28_02720"
     CDS             complement(<1..552)
                     /locus_tag="VNW28_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXA08866.1"
                     /translation="MRIGIAQINPTVGDLRGNFDKILAAYQRLAAGGAELILAPELAT
                     TGYPPQDLVFKSRFVPQNLETVDLLHASIGPVPLLVGYVDRNEGRGKPFFNAAALLER
                     GQPIRKTFKTLLPTYDVFDEDRYFEPGNGGEVFTIAGRRIGITICEDIWTESYLPRPL
                     YDLELARGLVEKGAEVIVNISASP"
     gene            complement(609..764)
                     /locus_tag="VNW28_02725"
     CDS             complement(609..764)
                     /locus_tag="VNW28_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08867.1"
                     /translation="MRATFIQKILICMIAGFFAACLIQWGKVFIEGADDALNGGYKKE
                     PTWSAGH"
     gene            complement(903..1601)
                     /locus_tag="VNW28_02730"
     CDS             complement(903..1601)
                     /locus_tag="VNW28_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAAX prenyl protease-related protein"
                     /protein_id="HXA08868.1"
                     /translation="MQEPWSSAAPARRSLLAHLLPMLFFVLLLGTGNALKTPGAALWR
                     AAPEFWIYPLQTFLGAGLLICFRRQYEFHRLRRPVFAIAIAFFVFVLWVAPQQFFHFP
                     ARVAGFNPDTFAAEPVVYWTTLAVRFLRLVVVVPLIEEIFWRGFLLRYLIADKFEQIP
                     FGAFSWYSFAIVTLAFGFSHSMADWPAAFITGALYNVVAYRTKSLTSCVLAHGLTNLA
                     LGLWIVTTKQWGFW"
     gene            1707..1970
                     /locus_tag="VNW28_02735"
     CDS             1707..1970
                     /locus_tag="VNW28_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08869.1"
                     /translation="MAGANEPEPDPPVADPEAVAKALEMELTLKRAAWQKARAKRGTW
                     RALSFLFLFLVVLGALLAYFYFATEVRRRGPESPRADAADSSR"
     gene            2120..4426
                     /locus_tag="VNW28_02740"
     CDS             2120..4426
                     /locus_tag="VNW28_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA08870.1"
                     /translation="MGLLRIVHLETDDADAELVRAELQTGAVECELIRVRSVRALLEE
                     ITQRPVDIILAERHGPGYDGLRALSMAREKRPGVPFIFVSRTDDSGQVGESLGAGATD
                     FIRKEHLPRLVPSLRRVIHEAHTQRDLQKVRQELLQHAELLDLANDAIVISDAAGKIS
                     YWNRGAERMYGWAREEAAGRDVHALLQSGPPEALVKVIRALQKKQHWEGELRQTRRDG
                     VEIVASTGWTLQGTAPDAPLLQLSVDVTDRIRAQEALRRSEERYRQFVDEDLTGNLIM
                     KPDGSIITCNPAFAQIFGFDSIEEAQAANFLSLLHTKKEGADLLASLRPNESTDRHEI
                     EMRQRDGDSVYVAARFIGSFDAAGRLIELKGYLFNDTKRKRLEQQLIQAQKMEGLGTL
                     AGGIAHDFNNILAIILGNTMRLEDWKKHPEQMPEAIKVVRDAVARGASLVQQLLTSAR
                     QTEARFTALDLNALVQELEKMLAATFPKTINFVLQLESHLPPAKADRSQIHQVLLNLC
                     VNARDAIPRDGTITLVTGVTAGDELREYFSGVNAERYIFVRVIDNGTGISERVKPHIF
                     EPFYTTKERSKGTGLGLSVVYGVVNNHRGFVQVESEPGHGTTFSVYLPLSSATEPASG
                     GDGSVGSPHDPARTIMLVEDEEMLRELGVMMLEGDGYRVLAAKDGIEAVEMFEVHADE
                     IGLVVCDLGLPRLGGRDVFLRMKEIKPSVRAIVASGYFEPNLRTEILRAGVLDTVQKP
                     YDFREMIEKIRSIVGQPQLEEDRQPQLF"
     gene            4434..5192
                     /locus_tag="VNW28_02745"
     CDS             4434..5192
                     /locus_tag="VNW28_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08871.1"
                     /translation="MTSCRPYHRTSLALGWEAARANLAPALLIQAAMLALLIGYYWNP
                     AVARVLQEVAEYKLRHGWAFVVLAAVAAGALVPEIFLVIFFQRGRPTRRNLRNLMFTV
                     PIWALDGSLVDLLYRGEAAWLGNVVTFPVVLAKIGLDQFGYNPFFAAPFGVLTYEWKN
                     SGFSLESLGRGFTWEHYRDKVVPTLLATWAVWIPLMAIIYSLPLALQFPLFGLALTFW
                     VLLLTYMTNSFAAKHIRKSRAAEVVSPAMAGPIR"
     gene            5189..>5669
                     /locus_tag="VNW28_02750"
     CDS             5189..>5669
                     /locus_tag="VNW28_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08872.1"
                     /translation="MKSHEIFQRMPPALGVEILTWLQKERTAVFKSAVQGLANQRHLR
                     AVFIERKPPNERYAWIQAALGRKLSDTLGAHLLQAWLLGAQKPMLCDFLDSLGITHDG
                     DGTVENLPASPPKEKLRASIDRLLARYPVEAVAVYLHAFHDMDTTVSWPPLGEVLAED
                     "
BASE COUNT         1118 a         1677 c         1825 g         1049 t
ORIGIN      
        1 cggagaggcg ctgatgttga cgataacctc tgcgcctttt tcaacgaggc cgcgcgccag
       61 ctcaaggtca tagagcggac gcggcaggta gctttcggtc caaatgtctt cgcaaatcgt
      121 aatgccgatg cggcgtccgg cgatcgtgaa gacttcgcca ccgttgccgg gctcgaaata
      181 gcgatcctca tcgaagacat cgtaggtggg gaggagcgtt ttgaaggtct tgcggatcgg
      241 ctgtccgcgc tcgagcaatg ccgctgcgtt gaagaacggc tttccgcgcc cttcgttgcg
      301 gtcgacgtag ccgaccagga gcgggaccgg gccgatggag gcgtgcagga gatcgaccgt
      361 ttcgagattt tgcgggacga agcgcgactt aaagacgagg tcctgaggcg gataaccagt
      421 cgtggccagt tcgggagcaa gaatcaactc tgcgccgccg gccgcgagtc gctggtaagc
      481 ggcgaggatt ttgtcaaaat tgccccgcag atcgccaacc gtcgggttga tctgggcgat
      541 gccgatcctc atgcgtctcc cgcagattaa ctgcgggtcc gccgagggca acggcttttg
      601 ggattagctc aatgcccggc cgaccaggtc ggttctttct tatagccgcc gttgagtgcg
      661 tcgtcggccc cttcgatgaa gacttttccc cattgaatca gacaagcggc aaaaaacccg
      721 gcgatcatgc agatgagtat tttctggata aaggtggcgc gcatgatcgc gtcttggaga
      781 gcaggagtgg tgccaatttc tcaggtccgg ccggcgggta gtgtcctaat ttgaagcggg
      841 ggcgaggtgc gccgtgaatt tggaaatccg cgccgcgctc cccagagaaa ggcgtcgaca
      901 ggtcaccaaa agccccattg tttcgtcgtc acaatccaga ggccaagtgc cagattcgtc
      961 aacccgtggg cgaggacgca agaggtgaga ctcttcgttc ggtaggcgac cacattgtag
     1021 agcgcgccgg tgatgaacgc ggctggccag tccgccatgg aatgcgagaa gccaaaagca
     1081 agcgtgacaa tggcgaagga ataccaggag aaggcgccga aaggaatttg ctcgaattta
     1141 tccgcgatca ggtaacgaag caggaacccg cgccaaaaga tttcctcgat caggggcaca
     1201 acgacgacga ggcgaaggaa gcgcaccgcc agtgtcgtcc aataaaccac cggctccgcg
     1261 gcgaaggtgt cgggattgaa gccggccacg cgtgctggaa aatgaaagaa ctgctgcggc
     1321 gcgacccaga ggacaaagac gaagaaggcg atcgcgatgg cgaagactgg gcgacgcagg
     1381 cgatggaact cgtactgacg acggaaacag atcaggagcc cggcgccgag gaaagtttgc
     1441 aatgggtaaa tccaaaactc aggcgcggcc cgccagagcg ccgcgcccgg cgtcttgagg
     1501 gcgttgcccg ttccgaggag caagacgaag aagagcatcg ggaggagatg ggccagcaag
     1561 gagcgcctgg ccggcgcggc ggagctccaa ggttcttgca tcggctgaag ctactcaagg
     1621 cggccggcgg tggcagcaaa aaggcgattc gaataccgct ggctccgcgg cattgcgcga
     1681 ggccgctgcc gtgtgaatgt tcgcccgtgg ccggagccaa cgaacccgaa cccgatccgc
     1741 ccgtggccga tcccgaggcg gtggcaaaag cgctggagat ggaattgact ctgaagcggg
     1801 ccgcgtggca aaaagcgcgg gccaagcgcg gcacctggcg cgcgctgagc tttctttttc
     1861 tgttcctcgt ggtgctggga gcgctcctgg cttatttcta tttcgcgacc gaggtgcgcc
     1921 gtcgcgggcc ggaatcgccg cgggcggacg ccgctgattc gagtcgttag gcaaagcgaa
     1981 ccgcggaatg ggactggcaa cgctcgcgct gatgtgcgac gagtgtagac cgccggggcc
     2041 agtcgcttcc gattatgcct aacgaacaat cgatgccact gcgcgctcgc ccggcgtccg
     2101 cccaaaaata attttcacga tgggtttatt gcggatcgtt catcttgaaa ccgacgacgc
     2161 tgacgccgag ctggtccgcg cggagttgca gaccggcgcg gtcgaatgcg agctgattcg
     2221 ggttcgaagc gtgcgggcgc tgctcgagga gatcacccag cgaccggttg atatcatcct
     2281 ggcagagcgt catggcccgg gatacgacgg tcttcgcgcc ctgtcgatgg cgcgggaaaa
     2341 acggccgggg gtgccgttta tctttgtctc gcggacggat gattccgggc aggtgggcga
     2401 gtcgcttggc gctggcgcga cggactttat tcggaaagag catctcccaa ggcttgtccc
     2461 gtcgctccgc cgcgtcatcc acgaggcgca cacgcaacgg gatttgcaga aggtgcgaca
     2521 ggagcttttg cagcatgcgg aactgctcga cctggcgaac gacgcgattg tcatttcgga
     2581 tgctgcggga aaaatctcct actggaatcg cggagcggag cggatgtacg gctgggcgcg
     2641 cgaggaggcg gcggggcggg atgtgcacgc gcttctgcag tcgggtccgc ccgaggctct
     2701 cgtgaaagtg attcgggcgc tgcagaaaaa acagcactgg gaaggggagc tgcggcagac
     2761 gcgccgcgat ggcgtggaga ttgtcgcttc cacgggctgg acgctgcagg ggacagcgcc
     2821 cgacgccccc ttactgcagc tcagcgtcga cgtgactgac cggatacggg cgcaggaggc
     2881 gctccgccgg agcgaggagc gttaccggca atttgtcgat gaagatctca ccggcaacct
     2941 gatcatgaag ccggacggtt cgatcatcac ctgcaaccct gccttcgcgc agatcttcgg
     3001 cttcgactcg atcgaagaag cgcaggccgc gaattttctt tctctgctgc acaccaagaa
     3061 agagggtgcc gatctgcttg ccagtctccg gcccaacgag agcacggacc gccacgaaat
     3121 cgagatgcga caacgggatg gcgatagcgt ttacgtggcc gcgcggttca tcggcagttt
     3181 cgatgcggcc ggccggctga tcgagctgaa gggctacctc ttcaacgaca ccaagcgcaa
     3241 acgcctcgag cagcaactca tccaggcgca gaaaatggaa ggcctcggca ccctggcggg
     3301 tggcatcgcc catgatttta acaacatcct cgccattatc ctcggcaaca cgatgcggct
     3361 cgaggattgg aaaaagcatc ccgagcaaat gcccgaggcc atcaaagtgg tgcgcgatgc
     3421 ggtggcgcgg ggagcctccc tcgtccagca attgctgacc tccgcgcgtc agaccgaggc
     3481 gcggttcacc gcgctcgatt tgaacgcgct cgtgcaggaa ctcgaaaaaa tgctcgccgc
     3541 gacgtttccc aagacaatca acttcgtgct ccaactggaa tcacatttac cgccggccaa
     3601 ggcggatcgg agccagatcc atcaggttct gctcaacctc tgtgtcaacg ctcgcgacgc
     3661 catcccgcgg gacggcacaa tcacgctcgt caccggggtc acggccggcg atgagctgcg
     3721 cgaatatttc agtggcgtaa acgccgagcg ctacattttc gtgcgcgtca tcgataacgg
     3781 caccggcatc agcgagcgcg tcaagccgca catcttcgag ccattttaca ccaccaagga
     3841 gcggagcaag gggaccggcc tggggctttc agtcgtgtat ggcgtggtca acaatcaccg
     3901 cggctttgtc caggtggaga gcgagccggg ccacggcacc acctttagcg tctatctgcc
     3961 gctgagcagc gcgaccgagc cggcctcggg cggcgacgga agcgtcggct cgccgcacga
     4021 tccggcccgc accatcatgc tggtcgagga cgaggaaatg ttgcgcgaac tcggggtcat
     4081 gatgctggaa ggagacggtt atcgcgtgct ggcggcgaag gatggcatcg aggcggtcga
     4141 gatgttcgaa gtgcatgccg acgagatcgg gctcgtcgtc tgcgatctcg ggctgccccg
     4201 gctgggtggg cgcgacgttt tcctgcggat gaaggagatc aagccgagtg tgcgggcgat
     4261 cgtggccagc ggctactttg agccgaacct gcgcacggaa atcttgcgcg ccggagtgct
     4321 cgacaccgtc caaaagccct acgattttcg cgaaatgatc gagaagattc gctctatcgt
     4381 cggccagccg cagcttgagg aagatcgtca gccgcagctt ttctgaggcg gcgatgactt
     4441 cgtgccgtcc gtatcatcgc acctccctcg cgctcggttg ggaagccgcc cgcgccaacc
     4501 tcgcaccggc gctcttgatc caggccgcga tgctggcctt gctcattggt tactattgga
     4561 atccggcggt tgcgcgcgtc ctccaggagg tggccgaata caaactgcgc cacggttggg
     4621 ccttcgtcgt tctcgcggcc gtcgccgcgg gggcgctcgt gccggaaatt tttctcgtca
     4681 tcttctttca gcgcggacgc cccacgcgcc gcaacctgcg caacctgatg ttcaccgttc
     4741 cgatctgggc gctcgatggt tcgctcgtcg acttgcttta ccggggcgaa gcggcctggc
     4801 tgggtaacgt tgtcactttt ccggtagtgc tggcgaagat tggcctcgat caattcggtt
     4861 acaatccctt cttcgccgcg ccttttggtg tcctgaccta cgaatggaaa aacagcggct
     4921 tcagcctgga gtcgttaggc cgcggtttta cctgggagca ttaccgcgac aaagtcgtgc
     4981 cgacgctcct cgccacctgg gcggtttgga tcccgctgat ggcgatcatc tactcgctcc
     5041 cgctcgccct gcagttcccg ctctttggcc tggcgctcac tttttgggtg cttctcctca
     5101 cttacatgac gaacagtttc gcggcgaaac acatccgcaa gtcccgggca gccgaagtgg
     5161 tttcgccggc gatggccggc ccgatccgat gaaatcccac gaaattttcc agcgcatgcc
     5221 gcccgccctc ggggtcgaga tcctgacctg gttgcaaaag gagcggacag cggtcttcaa
     5281 aagcgcggtc cagggtctgg ccaaccagcg ccatctgcgg gcggtgttca tcgagcgaaa
     5341 accgcccaac gagcgttacg cctggattca ggcggccctc ggtcgcaagc tcagtgacac
     5401 gctgggggct catctcctcc aggcctggct cctcggcgcg caaaaaccga tgctttgcga
     5461 ctttctcgat tcgttaggca tcacccacga tggagacgga acggtcgaga acctgcccgc
     5521 gtcgccgccc aaggaaaagc tgcgcgcgag tatcgaccgg ttgctcgccc gttaccccgt
     5581 cgaggcggtg gcggtctatc tgcacgcctt tcacgacatg gacacgaccg ttagctggcc
     5641 cccgttaggc gaggtcctgg cggaggacg
//
LOCUS       DATMNS010000072         6858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_806841, whole genome shotgun sequence.
ACCESSION   DATMNS010000072 DATMNS010000000
VERSION     DATMNS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806841"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..747)
                     /locus_tag="VNW28_02755"
     CDS             complement(349..747)
                     /locus_tag="VNW28_02755"
                     /inference="COORDINATES: protein motif:HMM:NF012260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HXA08873.1"
                     /translation="MSKTTKTELSPDNGAVIDTKWKVGPLGYHASAATPGATTPSGGG
                     FRLPEVIVYDHVGFKGAYARTNLSFHFLGNFWNDRISSLIVVNGVWRFYRDEYYKGDY
                     WDLGPGFYESFFTAAGPDDVVSSFQAISLT"
     gene            complement(844..1908)
                     /gene="glpX"
                     /locus_tag="VNW28_02760"
     CDS             complement(844..1908)
                     /gene="glpX"
                     /locus_tag="VNW28_02760"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HXA08874.1"
                     /translation="MRIPKQNYDDIERIIEFDFVRATEAAALNALPWLGRGEKEKADE
                     AACDAIRGMFDLMNICGEVVIGEGIKDHAPGIFKGEQLGMWLPGAPQFDIALDPIDGT
                     TNIAKGAPNSITCIAAATPEQGVKVALRDIPSFYMMKLAYGPDVIRSMKIAGIETLKL
                     ESPIEETLITVAKALNKRVQDVVVMMLDRPRHQHIVAEIRRVGASLRMISDGDIAAAM
                     APAYPESGIDLYVGIGGAPEAVLAAAGIKCLGGDMQCMMWPRDEKERAELVADGHEED
                     LGRVFFADDLANGKNIIFCATGISDSALLRGVKSQGANAITYSVLMRAKSKTVRFIRA
                     SHDLQSKTIRLRSDNREHRI"
     gene            complement(2037..4295)
                     /locus_tag="VNW28_02765"
     CDS             complement(2037..4295)
                     /locus_tag="VNW28_02765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXA08875.1"
                     /translation="MSNRILCAGLLFFIYLTISPLAPAAPAKRSITEKDLFAFVWLGH
                     PQLSPDGTRVAYVRVTVNEKKDGYDTSIWMVPTVGEEPARQLTSGPHDAGESWSPDGK
                     YLVFSRAAAEKEGKKQPPQLCLLPMAGGDAFVFTDLPKGAGDPIWSPDGKSILFTSTS
                     NPEDLAKQARQKKKEEEVKKAALAENSPGAKKEKPESPAKEEDEQESDVHVITQAVYR
                     DNDEGYLDPKRPQHLWLVTAPRSADEKVEPRQLTSGRFDEGNAFWSKDGARIYFTSLH
                     VDEPYYELPKTELYALPAKGGNAKLLTTIPMGVRALALSPDGKRAAFIAATGEPVNSY
                     TQPDLWTIDLTPSATPVNLTTGFDFDVGDSVFGDNAAPRGFGGGEPIWSEDGKSILEI
                     YSKEGRTQLASFNAENGAVTDLTHGNQAVLRYSASADGTKIVYLVSTPTQINDLFFID
                     QPGAEPRQLTHSNDALFGQLNLTEPEEIWYESFDGKRVQAWVQKPPGFDPTKKYPLIL
                     NIHGGPHAAYGYIFEHEFQWMAAQGYVVLYPNPRGSTSYGQMFGNIIQYKYPGDDFKD
                     LMAGVDEVVRRGGIDEKKMGVTGGSGGGLLTDWVVGQTDRFAAAVAQRDIADWADWWY
                     TADFTLFQANWFKTPPFEDKEGDYKARSPITYINNVKTPLMLVLGEDDSRTPPGAGGE
                     QMFRALKFRKIPTVMVKFPGETHELSRSGQPWHRIERLDHIVGWFDHWLKGIAKPEYE
                     VAPKEEVSAKKE"
     gene            4685..4897
                     /locus_tag="VNW28_02770"
     CDS             4685..4897
                     /locus_tag="VNW28_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08876.1"
                     /translation="MFRPRLSPLAALAGLLIVFTSCERHHVGELPELQREHLHPLAAQ
                     AEEAQPPAPPASSSPTPADFFPKQQP"
     gene            4931..6427
                     /locus_tag="VNW28_02775"
     CDS             4931..6427
                     /locus_tag="VNW28_02775"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POT family MFS transporter"
                     /protein_id="HXA08877.1"
                     /translation="MAKTPYLTTPPKITTMPPGVPYIVGNEAAERFSYYGMNSILTIF
                     MTKYLLDRMGHLSTMPPANAEAWYHTFVSTLYFLPIFGAVLADAVFGKFWVVFWISIV
                     YCAGHAMLALIGTPIAHTIEPRYLLAIGLILIAIGAGGIKPCVSTNVGDQFGESNKHL
                     LTRVFNWFYFSINAGSFISTLLIPWLLEPYQADPNGFIARLGPSVVGFLQSPRLHSAD
                     IAFGLPGVFMAIATLVFWLGRKKFVHIPPVGLKTYAREIFNKQTGKIILNVLMPVPFV
                     MIFWALWQQNFSSWIIQAESMDRHLFGIEWLSSQIQTVNPIFILIMLPVFSYWLYPFL
                     EKFVRLTPLRKIGAGLFVTAASFFIVAMIQTRIDAGARPSIIWQVWAFVVLTAGETLV
                     SPTHLEFSYTQGPVKLKSLIMCTYLLAISLGNQFTAAVNFFIQNPDGSVKLQGASYFM
                     FFVWVMLGTAVLFTIVSPFYKGRTYLQDQTRVTGDAEPGVGFAVQPEA"
     gene            6543..>6858
                     /locus_tag="VNW28_02780"
     CDS             6543..>6858
                     /locus_tag="VNW28_02780"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08878.1"
                     /translation="MPPPNEEYVLDLLRAVGLVTRKQIDEARDNVNGERSVIDVLVRQ
                     GLVSADDVSRSVAAEAEMSWIDLADIVVPQDVIDEIRPQDARRFKMIPIARNEQGLVV
                     ATG"
BASE COUNT         1187 a         2093 c         2008 g         1570 t
ORIGIN      
        1 ttttgaatcg ccgcagcgct ttcttgcctc gaaggagtgc ttcttgcggg cggaagtgcc
       61 ctgagggacg cgaaagggag gtcgacccgc cgcctcttaa aaccactctc gctgccccgt
      121 gccgtgaggg cggatggtca cactttggaa aaaatcagct accgggcagg cagtccggga
      181 aacgaaacgg agtctcacgg acattcgcgt ctcacaaata tccgtccgct gatgtttctg
      241 tcgcttccct tgcggccgtg gcgaagaggt ttagccgcga cgctcaacat ataccttcgc
      301 agagcgaggc gtctggggcg ggcgacactc agcgacgttg ccgactcgtt aggtcagcga
      361 aattgcctgg aacgaactga cgacgtcatc cgggccggcc gcggtgaaga acgactcgta
      421 gaatccgggg ccgaggtccc aataatcgcc tttgtagtat tcgtcgcggt agaaacgcca
      481 gacaccgttg accacgatca acgagctgat gcgatcattc caaaagttgc cgagaaagtg
      541 gaagctcagg ttggtccgcg cgtaggcgcc tttgaatccc acgtgatcgt aaacgatgac
      601 ctccggcaga cgaaaacctc ccccggacgg cgtcgttgcc ccgggggtcg ccgccgaggc
      661 gtgatagccc agcgggccaa ctttccattt cgtgtcaatc acagcgccat tatccggcga
      721 cagctcggtc ttggttgttt tgctcataag cggctttcgt tatctagatt tgaacagacc
      781 attctctgct tgtccagcgc gaggttttcg ttgcacgtga attttcgttc cgcgccctgc
      841 caatcatatg cggtgttcgc gattatcgga acgcagccgg atcgtcttgc tctggagatc
      901 gtgcgaggcg cggatgaaac gcacggtttt actcttcgcg cgcatcagga cggaataggt
      961 gatggcgttg gccccttgcg atttcacgcc gcgcagcagc gcgctgtcac tgattccagt
     1021 cgcgcaaaag ataatatttt ttccgttcgc gagatcgtcg gcgaaaaaga cgcgcccgag
     1081 atcttcttcg tggccatcgg cgacgagttc ggcgcgctct ttttcgtcgc gcggccacat
     1141 catgcattgc atgtcgccgc cgaggcattt aatcccggct gcggctagca ccgcttcggg
     1201 tgcgccgccg attccgacgt aaagatcgat cccgctctcc gggtaagccg gggccatggc
     1261 cgctgcgatg tcaccgtcgc tgatcatgcg gagcgaggcg ccgacgcgcc ggatttccgc
     1321 gacgatgtgt tggtggcgcg ggcggtcgag catcatcacc accacatcct gcacgcgctt
     1381 gttcaacgcc ttcgccaccg tgatgagcgt ctcctcgatc gggctttcca gtttcagggt
     1441 ttcaatccca gcgatcttca tgctccggat gacgtccggg ccgtaggcca gcttcatcat
     1501 gtaaaacgac gggatgtcgc ggagcgcgac cttgactcct tgttcggggg tcgctgccgc
     1561 aatgcaggtg atcgagttgg gcgcgccctt ggcgatgttg gtcgtgccgt cgatcgggtc
     1621 gagcgcgatg tcgaattgcg gcgcgcccgg cagccacatc ccgagttgtt cgcctttgaa
     1681 aatgccgggc gcgtggtcct tgatcccttc gccgattacg acttcgccgc agatgttcat
     1741 caggtcgaac atcccgcgga tcgcgtcgca ggcggcttca tcggcttttt ccttctcgcc
     1801 gcggccgagc cagggaaggg cgttgagcgc ggcggcctcg gttgccctaa cgaaatcgaa
     1861 ttcgatgatg cgctcgatgt cgtcgtaatt ctgtttcggg atgcgcatcg gctgatgttc
     1921 gcaaaggtta ggcagcgtga caagcgcgct tcgggcaggc ttatttttga caggatgaac
     1981 aggattttca ggatggaaga agatcgcggc gcttcttcca atccagttaa tcctgtctat
     2041 tccttctttg cggacacttc ctccttcggc gccacttcgt attccggttt cgcgatgcct
     2101 ttcagccagt gatcgaacca gccaacgata tggtcgagcc gctcgatccg atgccacggt
     2161 tgcccggagc gtgacagctc gtgggtctcg ccagggaatt tcaccatcac cgtcgggatc
     2221 ttgcgaaact tgagcgcgcg aaacatctgt tcgccccccg cgcccggcgg ggtgcgcgag
     2281 tcgtcttcgc cgagcacgag catgagcggc gtcttcacgt tgttgatgta ggtgatcggc
     2341 gagcgcgcct tgtaatcgcc ctctttgtcc tcgaagggcg gggtcttgaa ccagttcgcc
     2401 tggaagagcg tgaagtccgc ggtgtaccac cagtcggccc agtccgcgat gtcgcgttgc
     2461 gccacggcgg cagcgaaacg gtcggtctgt ccgacgaccc agtccgtcag caggccgccg
     2521 ccgctcccgc cggtcacccc catttttttc tcgtcgatcc caccccgcct aacgacttcg
     2581 tccacgcccg ccatcaggtc tttgaaatcg tcgcccggat atttgtattg gatgatattg
     2641 ccgaacatct ggccgtagct cgtgctcccg cgcgggttgg gataaagcac cacgtagcct
     2701 tgtgccgcca tccattggaa ttcgtgctcg aagatgtagc cgtaggctgc gtgcgggcct
     2761 ccgtggatgt tgaggatgag cggatatttt ttcgtcggat cgaagcccgg cggtttctgc
     2821 acccaggcct gaacgcgttt gccgtcgaag ctctcgtacc agatttcttc cggctcggtc
     2881 agattcagtt ggccgaagag cgcgtcattg ctatgcgtca actggcgcgg ctccgcgccc
     2941 ggctgatcga tgaagaacaa gtcgttgatc tgcgtcggcg tggaaacgag gtagacaatt
     3001 ttcgtcccgt cggccgaggc ggaatagcgc agcaccgcct ggttgccgtg cgtcaggtcg
     3061 gtgacggcgc cgttttccgc attgaatgag gccagttggg tgcgcccttc cttcgaataa
     3121 atctcgagga tgcttttgcc gtcctccgac cagattggct cgccgccgcc gaacccgcgc
     3181 ggtgcggcat tatccccaaa gacgctgtcg ccaacatcga aatcgaagcc ggtcgtgaga
     3241 ttgaccggcg tagcgctcgg tgtgaggtcg atcgtccaga gatccggttg cgtgtaggag
     3301 ttgaccggct cacccgtcgc ggcgatgaac gccgcgcgct tgccatccgg gctaagcgcg
     3361 agcgcgcgga cacccatcgg aatggtcgtc aataatttcg cgttgccgcc tttcgcggga
     3421 agcgcataga gctcggtctt gggcagttcg tagtagggct cgtccacatg gagggaggtg
     3481 aaataaatcc gcgcgccatc cttcgaccaa aaggcgttgc cctcgtcaaa gcgaccgctc
     3541 gtcaactggc gcggttcgac cttttcgtcc gcgctgcgcg gcgccgtgac cagccaaaga
     3601 tgctggggac gtttcgggtc gaggtatccc tcgtcgttgt cccgataaac ggcctgggtg
     3661 atgacgtgca cgtcgctttc ttgttcgtct tcctccttgg ccgggctctc cggcttttcc
     3721 ttcttcgcgc cgggcgagtt ttccgcgagg gcggctttct tcacctcttc ttccttcttt
     3781 ttttgtcgtg cctgtttcgc gaggtcttcc ggattcgaag tgctggtgaa gaggatcgac
     3841 ttgccatccg gcgaccagat cgggtcgccc gcgcctttcg gcagatcggt aaaaacaaac
     3901 gcgtcgccgc ccgccatcgg caggaggcaa agttgcggcg gctgtttctt gccctccttt
     3961 tccgcagcgg cgcgactaaa gacgagatac tttccatcgg gcgaccagct ttccccggca
     4021 tcgtgcggac cactcgtcag ttgccgggcc ggttcctcgc ccacggtcgg caccatccag
     4081 atggaagtgt cgtagccgtc ctttttttcg ttcaccgtta cccgtacgta ggcgacgcgc
     4141 gtgccgtcgg gcgagagctg cgggtggccg agccagacga aagcgaagag atctttttct
     4201 gtgatgctgc gtttcgcggg tgcggccggc gcgaggggcg agatcgttag gtaaatgaaa
     4261 aatagaagac cagcgcacag gattcggttc gacatgcgcc taagctggca ctgtgcgcgc
     4321 ttggcgcaat cgacgcgcat ttgttcgggt agcgcacgcg tctcgcgtgc tggcgaaggc
     4381 gtcccgcgtt cgcgaacttt tctgccgcct gcgcctatcg taatgacgcc gccatacgga
     4441 aagttcgtcg ccgcagagac gcggcgacca gcacccgaga cggggcgcta cccgaaagcc
     4501 ctgtcgccgc tcgtcgcttg tagcctaacg ggattcggtt cttcatgcgc ctgccgattt
     4561 gcacgatcgc tctccccaat ctcgacggag tctcgcccta ccggaacagc cggcgtcccc
     4621 gcgctttcat cccgccaatc tttttgttgc gccccgcgct tcccgcccct taatctgctc
     4681 ccgcatgttt cgtcctcggt tgtctccgct cgcggcgctt gccggtttgc tcatcgtctt
     4741 cacttcctgc gagcgtcacc acgtcggcga gttgccggaa ttgcaacggg agcacctcca
     4801 tcctctcgcg gcgcaggcgg aagaggccca gccgcccgcg cctccggctt cttcttcccc
     4861 gaccccggcg gactttttcc cgaagcagca gccctgacga gtcgatcgcg ccctaacgaa
     4921 aagcagcgac atggccaaaa cgccgtacct caccacgcca ccgaagatca cgaccatgcc
     4981 tccgggcgtg ccctacatcg tcggcaacga ggcggccgag cgcttctcct attacgggat
     5041 gaattccatc ctgaccatct tcatgaccaa gtatctcttg gacaggatgg ggcacctgag
     5101 caccatgccg cccgccaatg ccgaggcctg gtatcacacc tttgtctcga cgctttattt
     5161 tctgccgatc ttcggcgcgg tcctggccga cgcggtcttt gggaaattct gggtcgtttt
     5221 ctggatctcg attgtttact gcgccggcca cgcgatgctc gctttgattg gaacgccgat
     5281 cgcgcacacg atcgagccgc gttatttgct cgcgattggt ttgatcttaa tcgccattgg
     5341 cgccggcgga ataaaaccgt gtgtctcgac caatgtcgga gaccaatttg gcgaatcgaa
     5401 caagcatttg ctgacccggg tcttcaattg gttttatttt tcgatcaacg ccggctcatt
     5461 catttcgacg ctcctgattc cgtggttgct cgagccctac caggctgatc cgaacggttt
     5521 catcgcaaga ctggggccgt cggtggtcgg ttttctgcaa agcccgcgcc tccacagtgc
     5581 ggacattgcc ttcggtctcc ccggcgtctt catggcgatt gcgacgctcg tcttttggct
     5641 tggtcgcaaa aaatttgtcc atattccgcc cgtcggtttg aagacctacg cgcgtgagat
     5701 tttcaacaag cagaccggca agatcatcct caacgtcctg atgccggtcc catttgtgat
     5761 gatcttctgg gcgctctggc agcagaactt ctcctcctgg attatccagg cggagtcgat
     5821 ggatcggcac ctcttcggga tcgagtggct ttcttcgcag atccagacgg tgaacccgat
     5881 ctttatcttg atcatgctgc cggtattttc ctactggctc tatccgtttc ttgagaagtt
     5941 cgtccggctg acgccgctcc gaaaaatcgg ggccggatta ttcgtcaccg ccgcttcttt
     6001 ttttatcgtc gccatgattc agacgcgcat cgacgccggc gctcgaccga gcatcatctg
     6061 gcaggtctgg gcgtttgtcg tgctgacggc gggcgaaacc ctcgtctcac cgactcacct
     6121 tgagttttct tacacacaag ggccggtgaa actgaaatcc ctcatcatgt gcacctatct
     6181 gctcgccatt tcgctcggca atcaattcac ggccgccgtc aatttcttca ttcagaatcc
     6241 ggacggctcg gtgaaattgc agggcgcgag ttacttcatg ttcttcgtct gggttatgct
     6301 gggaacggcg gtcttgttca cgatcgtgtc tccgttttac aagggtcgca cctatttgca
     6361 ggatcagacg cgtgtgaccg gcgatgcgga gccgggggtc ggattcgcgg tccagccgga
     6421 agcgtagcga gaccgcgagg aatatcgcgt tgccgggcgt ctgatccacc tcggaaggga
     6481 gcgccgagcc accgacttcc gccttttctt ttcgcgccgc gaccgcgatc atcttcctgc
     6541 gcatgccccc gccgaacgaa gaatatgttc tcgatctcct gcgcgcggtg ggcttggtga
     6601 cccgcaagca gattgacgaa gcgcgcgaca acgttaacgg cgaaagaagc gtgattgacg
     6661 ttctcgtcag gcagggactg gtttcggccg acgacgtttc ccgaagtgtc gctgccgagg
     6721 ccgagatgag ctggatcgat ctcgccgaca tcgtcgtccc gcaggacgtg atcgacgaaa
     6781 tcaggccgca ggacgcgcgc cgtttcaaaa tgatcccgat cgcgcgcaat gagcagggcc
     6841 tcgtcgtcgc gaccggag
//
LOCUS       DATMNS010000073         2496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1526115, whole genome shotgun sequence.
ACCESSION   DATMNS010000073 DATMNS010000000
VERSION     DATMNS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1526115"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2496)
                     /locus_tag="VNW28_02785"
     CDS             complement(<1..>2496)
                     /locus_tag="VNW28_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HXA08879.1"
                     /translation="PGRMLLAEANQWPEDAVKYFGNGDESHMNFHFPLMPRMFMALQM
                     EDRFPIIDILEQTPAIPENCQWAMFLRNHDELTLEMVTDEERDYMWRVYANDPTARIN
                     LGIRRRLAPLLANNRRKIELLNILLFSMPGTPVLYYGDEIGMGDNFYLGDRNGCRTPM
                     QWSSDQNAGFSKANPQQLYLPITIDPEYHYEAINVENQQKNLSSLLWWTRRVIAMRKN
                     FRAFSRGSLEFLFPDNSKVLAFLRRHENETILVVANLSRFSQAAEIDLSRFAGCAPME
                     VFSRNIFPTIRKARYLLTIGPHGHFWFILQSQGGKRPLKKRVVPSLAVEANLQSLLAD
                     GQRTRLERFILPEYLNTCRWFGAKARKIREIRIIEQIPVAENAAQFWLLQVGYTDGAP
                     DIYSLPVQVATGAAADALARHSPQAVIARLGPQQAVLYDAIWDADFRAQLFQLMATAA
                     NLKGKSGELIGVTSSLLAQEPNENTPSSQVLNAEQSNSSMLFENRFFLKLYRKLEDGV
                     NPDVEVTRFLTERRQFANVPAFVGAIEYRQSRAEPTVVALLQTAVPNEGDAWKLTLDA
                     VGRYFERVLARKGDLQAAAIAPGPVLDELLGGVYRAKATLLGQRTAGMHLALGSEPED
                     RVFAPEPFNAMAQRSVYQSMRASLRRAFALLQKKLPELPEAFRVEAAQVLKAEPEILA
                     NEQRLLDQTAPASKIRIHGDYHLGQVLYTGKDFVILDFEGEPARPLSERKLKRSALRD
                     VAGMMRSFQYAAYSALWQPSTRAEDTPFLEHWADLWYREMSATFLASYLRTAAGATFL
                     PQKESDLRVLLEAYLLDKAVYEVGYELNHRPDW"
BASE COUNT          502 a          755 c          758 g          481 t
ORIGIN      
        1 cccaatcggg tcggtgattg agctcatagc cgacctcgta gaccgctttg tcgaggaggt
       61 aggcttccaa gagcaccctc aggtcgcttt ccttttgcgg cagaaaggtc gcacccgccg
      121 cggtccgcag atagctcgcg agaaaagtcg cgctcatctc gcgataccag agatcggccc
      181 agtgttcgag gaagggtgtg tcttcggcac gggtcgatgg ttgccagagc gcgctgtagg
      241 cggcgtattg aaaagagcgc atcatgcccg ccacatcgcg caacgcggaa cgtttcaact
      301 tccgttcact caagggcctt gccggctcgc cttcgaaatc gagaatgacg aaatccttac
      361 cggtgtaaag cacctggccg agatgataat cgccgtggat gcggatcttg ctcgccggcg
      421 ccgtttgatc gagcaaccgc tgttcgttgg ccagaatctc cggctcggcc ttgagcacct
      481 gcgccgcttc cacgcggaag gcctccggca attcgggcag ttttttttgc aggagcgcaa
      541 aggcccggcg caacgacgcg cgcatcgatt gataaacgga gcgctgcgcc atcgcgttga
      601 acggctccgg cgcaaaaacc ctgtcctccg gctcggagcc cagcgcgagg tgcatcccgg
      661 cggtgcgctg gccgagcaac gtcgctttcg cgcgatagac gccgccaagc aattcgtcca
      721 gcaccgggcc gggagcgatg gccgcggcct gcaggtcgcc tttgcgcgcc agcacccgct
      781 caaagtaacg gccgaccgca tccagagtga gcttccaggc gtcgccctcg ttaggcacag
      841 ccgtctgaag cagcgccaca accgtcggtt cggcgcgcga ctggcgatat tcgatcgcgc
      901 cgacgaaggc gggcacattg gcaaactgcc gccgctcggt caggaagcgc gtcacctcca
      961 cgtcgggatt cacaccgtcc tccagtttgc ggtagagctt caggaagaaa cgattctcga
     1021 agagcatcga cgagttgctc tgctcggcgt tgagcacctg cgaggaaggc gtgttctcgt
     1081 taggctcctg cgccagcaga ctgctcgtca cgccgatcag ttcgccgctt ttgcctttca
     1141 ggttggccgc tgtcgccatc aattggaaaa gctgcgcgcg aaaatcggca tcccaaatcg
     1201 catcgtagag gacggcttgc tgcggaccga ggcgggcaat gaccgcctgc ggagaatggc
     1261 gggcgagcgc atccgccgcc gcgccggtcg ccacttgcac gggcagcgaa tagatatccg
     1321 gcgcgccgtc cgtgtagccg acctgcaaaa gccaaaattg cgcggcgttt tccgcgactg
     1381 gaatctgttc gatgatccgt atctcgcgaa tctttctcgc cttcgcgcca aaccagcgac
     1441 aggtattcaa gtactcgggc aaaatgaagc gctccagccg cgtgcgctgg ccgtcggcga
     1501 gcagagattg caaattcgcc tcgactgcga gcgacggcac gacccgcttc ttgagcgggc
     1561 gctttccgcc ctgcgattgc aggatgaacc agaaatggcc atgcgggccg atcgtcagca
     1621 ggtaacgcgc tttccggatc gtcgggaaaa tgttccggct gaaaacttcc atcggcgcgc
     1681 aaccggcgaa gcgtgacaaa tcgatctccg ccgcctggga gaagcgcgac aaattcgcga
     1741 ccacgaggat ggtctcgttc tcatggcgac gcaaaaaagc gagcaccttt gagttatcag
     1801 ggaaaagaaa ttcgagcgac ccgcgggaga aagcgcggaa gtttttccgc atcgcgatca
     1861 cgcggcgcgt ccaccaaaga agcgacgaca aattcttttg ctggttctcg acgttgatcg
     1921 cctcgtagtg atactcgggg tcgatcgtga tcggcaggta gagctgttgc ggattcgctt
     1981 tcgaaaaacc ggcattctga tcgctgctcc actgcatcgg ggtgcggcag ccgttgcggt
     2041 cgccgagata aaagttatcg cccatcccga tctcgtcgcc gtagtagagc accggcgtgc
     2101 ccggcatcga gaaaagcagg atgttgagca gctcgatttt gcgccggtta ttggcgagga
     2161 gcggcgcaag ccggcggcga atcccaagat tgatccgcgc ggtcgggtca ttggcgtaga
     2221 cgcgccacat gtagtcgcgc tcttcgtcgg tcaccatctc gagcgtcagt tcgtcgtggt
     2281 tgcgcaggaa catcgcccac tggcaattct ctggaatcgc cggcgtctgc tcgagaatat
     2341 cgatgatcgg aaatcgatcc tccatttgca gtgccatgaa catccgcggc atgagcggga
     2401 aatggaaatt catgtgcgac tcatcaccgt tgccgaaata tttgaccgca tcctccggcc
     2461 actgattcgc ctcggcgaga agcattctgc cgggga
//
LOCUS       DATMNS010000074         2144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_98215, whole genome shotgun sequence.
ACCESSION   DATMNS010000074 DATMNS010000000
VERSION     DATMNS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98215"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VNW28_02790"
     CDS             complement(<1..259)
                     /locus_tag="VNW28_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08880.1"
                     /translation="MNAEILDTKIVDGIAVVTLGSPKRLYFDAEMGDALTGALETFAG
                     DHCVRVVIVTGGAPGYFNRHFDIPSLIRIAEDLRSSGREWPE"
     gene            complement(336..1157)
                     /locus_tag="VNW28_02795"
     CDS             complement(336..1157)
                     /locus_tag="VNW28_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXA08881.1"
                     /translation="MSTTNPLRVHRETSAYWRVVFDYPPFNVLDGTVFQALQDLLEQI
                     EANSALRAVVFESANADFFLSHFDLTGKLGNVMSAVGPTGLPILLDTFVRISKSPVAM
                     IAKIRGCVRGACSEFVLACDMRFASRENTRLGQPEVGVGLHPGGGGTERLPHLVGRGR
                     ALEIILGASDFDGETAERYGYVNRALPDAELDAFVDTLARRISSFDKRSIAAAKRLVN
                     EISLPPADRFLDAFNSFGTALSWPETQTKVGELLKRGLQTNTEFEKRWPEVLDTL"
     gene            complement(1367..>2144)
                     /locus_tag="VNW28_02800"
     CDS             complement(1367..>2144)
                     /locus_tag="VNW28_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08882.1"
                     /translation="LVTAALAVALAMPGAAAVKKIAYPEVKVTLNEPYKPDPAFEKMH
                     SQFLDAIKCKDLQALTALVAPTFLWTVGDTPADELDLGRDAIHNFKVAFGFRGLGKDA
                     DGGVDNGPYWDVLASFADDATYYAATDSGNLVCGPIAAEVADDRIFEQARKRIETANE
                     AAEWYFTLAETAVAKAPSDTGAPVAKVGIVALPLLGFYPPEKQGAPPPAPTHFEVLLP
                     SGRTLWLPVTAVRPLSADHLCYARTPSGDWKIAAIDQASQ"
BASE COUNT          398 a          650 c          675 g          421 t
ORIGIN      
        1 tttccggcca ttcgcggccg gaggaacgaa ggtcttctgc gatccggatg agagaaggga
       61 tgtcaaagtg gcggttgaaa tagccaggcg caccgccagt cacgattacc acgcgaacgc
      121 aatgatcccc tgcgaacgtc tcaagcgccc ccgtgagggc gtcccccatt tcggcatcga
      181 aatacaagcg cttgggactg cccaaggtca caacagcgat cccgtcaacg atcttagtat
      241 cgagaatctc ggcgttcatc ttgatctccc gaatttgatc agggctgggt tactgcggga
      301 atttatggcg cgcgcgtgcc ggaaaacccg gcatctcaaa gggtgtccag cacctccggc
      361 catcgttttt cgaactctgt gttggtttga agcccgcgct ttagcaactc tccgaccttc
      421 gtctgggtct ctggccagct cagcgctgtc ccgaaggagt tgaaggcgtc caggaaacgg
      481 tcggcgggcg gcaacgaaat ctcattgacc agacgtttcg ctgccgcgat ggatcgctta
      541 tcgaaagaac tgatccgccg cgcaagtgtg tcaacgaatg catcgagctc ggcgtcgggc
      601 agcgcgcggt tgacatatcc gtatcgctcg gctgtttcac cgtcgaagtc gctcgcgccc
      661 agaataatct caagcgcgcg accccttccc actaagtgtg gaagtcgttc agtcccgcca
      721 ccaccaggat ggagtcctac cccgacctcg ggctggccaa gccgagtgtt ttctctcgaa
      781 gcgaagcgca tgtcacaggc gagaacgaac tcgctgcacg ctcctctaac gcaacctcgg
      841 attttcgcga tcatcgcgac cggcgatttg gagatccgga cgaaggtatc gagcaggatg
      901 ggcagtcccg tggggccgac cgcgctcatg acgttgccga gcttgcccgt caaatcaaag
      961 tgcgaaagga agaagtcggc atttgcgctt tcgaaaacaa ccgcgcgcaa cgccgagttg
     1021 gcttcgatct gttcgagcag atcctggagc gcttgaaaca ccgtcccgtc aaggacgttg
     1081 aacggcgggt agtcgaaaac gactcgccaa tacgcggagg tctcccgatg gacccgcaat
     1141 ggattcgtgg tgctcattgt tgctcgtttc ctctactgcg gatcgtcagt gaaattgttt
     1201 tcggagaagc cgccattgcg attcgcggcg cgcccagact cctcccccgc ccccttttag
     1261 ggtaggcggg tccggttcac caggccatgt cattgttgcc accatttcgg ccgcgggatc
     1321 gcattgcggc gaaaatcgag gctgcttccg gatgtcaacc agaccgctat tggctcgcct
     1381 ggtcgatggc ggcgatcttc cagtcgccgc ttggggtgcg ggcgtagcag agatgatcgg
     1441 cgctgagcgg gcgcaccgcg gtcacaggca gccagagggt gcgccccgac ggcagcagca
     1501 cctcgaaatg ggtcggcgcc ggcggcggtg cgccttgctt ttccggcgga tagaagccga
     1561 gcaagggcag cgccacgatc ccgaccttgg cgaccggcgc gccggtgtcg ctgggggcct
     1621 tggcgacggc ggtttcggcc agggtgaaat accactcggc ggcctcgttg gcggtttcga
     1681 tcctcttgcg ggcctgctcg aaaatcctgt cgtcggcgac ttcggcggcg atcggcccgc
     1741 acacgagatt ccccgaatcg gtcgcggcat aataggtcgc gtcgtcggcg aagctcgcga
     1801 gcacgtccca ataggggcca ttgtcgacgc cgccgtcggc gtccttgccg agaccgcgga
     1861 agccgaacgc gaccttgaaa ttgtggatgg cgtcgcggcc gagatcgagt tcgtcggcgg
     1921 gcgtgtcgcc cacggtccac aggaaggtcg gcgccaccag cgccgtcagc gcctgcaggt
     1981 ccttgcactt gatcgcgtcg aggaattggc tgtgcatctt ctcgaaggcg gggtccggct
     2041 tatagggctc gttgagagtg accttgactt cggggtaggc gattttcttc accgcagcgg
     2101 caccgggcat cgcaagcgcg actgcgaggg ccgcggttac gagc
//
LOCUS       DATMNS010000075         8722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_100875, whole genome shotgun sequence.
ACCESSION   DATMNS010000075 DATMNS010000000
VERSION     DATMNS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100875"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1258
                     /locus_tag="VNW28_02805"
     CDS             <1..1258
                     /locus_tag="VNW28_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HXA08883.1"
                     /translation="LGLIGIMALWLGVMRLAERAGLIQKLARGLCPVMRRLFPGVPND
                     HPAIGSMLMNMAANMLGLGNAATPLGLRAMRDLERLNPHPGTATNAMCTFLAINTSSV
                     QLIPTTAIAILVAAGSKNPTAIVGTALLATLCAATVAITSVKLLEKLSWFRVPAERFA
                     AGKDDPGAAERTSDDSNVQPANLPSSGAVGITDPADTGHLRPWGKALLALFALFFLAL
                     FVRQIFPETLGLSPGTTAPAGSYFVRGVSALSLLAIPLLLSFFVLYAALRGLKVYDEF
                     VEGAKEGFGVILKIIPFLVTMLVAIGMFKGAGGIELLARALAPILTPLHFPTDLLPIA
                     LMRPLSGGATLALFTDVVHRLGPDNIVSLMAATIYGSTETTFYVAAVYFGSVGIKQTR
                     HAIPAGLLADFAGVVASVIICRAVLS"
     gene            1389..2570
                     /locus_tag="VNW28_02810"
     CDS             1389..2570
                     /locus_tag="VNW28_02810"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HXA08884.1"
                     /translation="MIQRDYLIIGSGIGGSSVCEGIRQFDKKGSVTLVGGETYYPYKR
                     WMLSKQFLREKTPALKKLPCLEERWFDSHKIETRFGTVVTQFNIDRRLAVLGNGETIE
                     FKKACLAMGSRPVRPMVAGIGLGNVIYLRTVRDALALREMAQSEKMIVIVGGGLLACE
                     VAASLRQAKLKIAIMHRHQSLLNRYLDPETSTWVTEQFAKKGVTLLLGSSLNGFEGKT
                     VLRNIQTKSGERVAAGVAVVACGSEPNLEIVRNTPLSGPHGSPVTDYLETEEKGIYAV
                     GDLAFYPDRLMGGMRRQTHWENAREQGLIVGANITGKKRQRYEQIPYFWTEMFDLKFD
                     FIGDFNVPATHVQLSGSYAKGRFALRYFQGEKLRGIVLCHHSAGEVNSARNELRQGLG
                     K"
     gene            complement(2582..3070)
                     /locus_tag="VNW28_02815"
     CDS             complement(2582..3070)
                     /locus_tag="VNW28_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08885.1"
                     /translation="MQSTFRIGLYAGIVVAAALGLWLAQLWQEENQVRLHSEHLLRKI
                     EARNWSGAGDFMAGDYRDDWDDDRTRVLNRLRLVLRSFASLTITAADPQVRLDPPTGN
                     WSAKIQIGGTGGEFVPEVVARVNSLTEPFELDWRRESWAPWDWKLIRVRNPALKISGQ
                     IY"
     gene            3280..4008
                     /locus_tag="VNW28_02820"
     CDS             3280..4008
                     /locus_tag="VNW28_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA08886.1"
                     /translation="MLDHDDLKWVATNATELNRLMQQVTRLSEQARQHKGEGNYLDLL
                     NEQAQQACKTSQALFDRITSRILYRAGSEMQTAEPSPSLPGTAAPSARTNVSERIPLG
                     EMKVETQSGDPPIRNPKGKRELVLVVDDDLDLLKVAADMLEFEDYRVILAKDGLEALQ
                     IYRRMGKQIGLIVLDYFLPVMDGDAVFDELKAIDPDVRVVLSSGFGEQAKLGSMLARG
                     LCGFLPKPYTHEKLIGHIQSIIAA"
     gene            4111..5193
                     /locus_tag="VNW28_02825"
     CDS             4111..5193
                     /locus_tag="VNW28_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXA08887.1"
                     /translation="MARIDAFFKLMSEEKASDLHLATGNPPMLRINGELVRVDYPPLQ
                     NDELKAMVYEIAPEVKIKVFEETSDVDFGYEVPGVARYRANFFQQKSGISAVFRLIPS
                     KILTVEELGLPPVLKKFPLLKKGLILVTGPTGSGKSTTLAAMMDYANLQRHDHIITVE
                     DPIEFVHRSQNCLVQHREVGVHTRSFASALRGALREDPDVLLVGEMRDRETIELALTA
                     AATGHLVFGTLHTSSAPKAVDRVIDVFPTDQQNQIRTTLAESLKGVIAQNLFKRIDKP
                     GRVAALEILVVDGAIANLIREAKTHQIPGMIQVGKKKGNQPLDDAIIEHLRQMRISPE
                     EAYDKAIDRKKFRPFLKTPPADEFED"
     gene            5205..6371
                     /locus_tag="VNW28_02830"
     CDS             5205..6371
                     /locus_tag="VNW28_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXA08888.1"
                     /translation="MRVPDLDRILSAMLKTYDGISDLNFSVGHPLQVEDYGELKPVFI
                     DPPIERLTTYQTEQLALVLMQGDRRLMRDYLVEGSCDCSYSLGGEARFRVNIFRQQGH
                     FAIVMRKLEAVVPSFRTLGLPLIFEEIAKEKAGLVLVTGATGSGKTTTLAAILNEINE
                     KLSVHVVTLEDPIEFIHPPARATFNQRELGHDFDNYPNGLRAALRQSPKVVFVGEMRD
                     RATVEVALMAAETGHLVLSTLHTVDAGQSINRILGLFSLGEEQQLRIRLADSLRYVVS
                     QRLAPKIGGGRYLLTEIMGHNLRTRETIALGEADNRSFYEIIEASSPFGWTTFDRSVL
                     SAYEAGVISEETARGFSSKKGKVIRGIDLIQKGRGIENETEGGLRLDLPVNAFR"
     gene            6381..6848
                     /locus_tag="VNW28_02835"
     CDS             6381..6848
                     /locus_tag="VNW28_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08889.1"
                     /translation="MGSDPDSNFFDLGDKTALVCIDHPQYQNMIVPQLTELNYKVHLG
                     LFEEDVLLKLATYSYNVVVVYENFKRSKVDDNPILREMVKRTNSLRREHFVILLSHRF
                     ATNDAMSAFVQSVDQIINITDIANFKPIMRRGVAQQRELYTPFQETLKAVRAS"
     gene            6946..7470
                     /locus_tag="VNW28_02840"
     CDS             6946..7470
                     /locus_tag="VNW28_02840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXA08890.1"
                     /translation="MKLVQWVRFTWDLDTLSLLGAVLPEHYNFVVATEADEKELRAVI
                     TRSFAHDTSWGDAIHEVNDMIDGWLLRAFAPGDGGGVCLALRHGLRMIGASILIPAPA
                     AENHLSPGPCVLMEYRNRGLGTALLGESLHRLREVGLARATARTKRNAPVAKFLYPKF
                     NGILTPGDTPLLAA"
     gene            complement(7503..8282)
                     /gene="lpxA"
                     /locus_tag="VNW28_02845"
     CDS             complement(7503..8282)
                     /gene="lpxA"
                     /locus_tag="VNW28_02845"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HXA08891.1"
                     /translation="MSETQIHPTAIVDPSAQLGTGTVIGPYCIVAAGVSLGENCWLQH
                     HVTLGGPLATGRGNKFYAYCSIGQQTQDLKYRGEPTYLEIGNENCFREFVTINRSTLE
                     SGKTRVGSGGNFLAYSHIGHDCTVGDSVIFSNNGTLAGHVQVGDHAVIGGLTAVHQFC
                     RIGRFAITGGCSKIVQDVPPFMIADGNPAEVRGINQVGLERHGYPPEKIKPIKEAFRL
                     IYRAKMNTGQALETVREQLGESEDVRSILQFIAASERGIIR"
     gene            complement(8432..>8722)
                     /locus_tag="VNW28_02850"
     CDS             complement(8432..>8722)
                     /locus_tag="VNW28_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HXA08892.1"
                     /translation="HFPGHPVMPGVLQVEAMAQVASVLMMRISKSASRVGYFMSADGV
                     KFRKPVFPGDTLFIHAELTKSRGNRLAKAMCRCVVNDAVVSEGELMFTFLDK"
BASE COUNT         1754 a         2676 c         2527 g         1765 t
ORIGIN      
        1 gctcggtttg atcggaatta tggcgctctg gttgggcgtg atgcgactgg cggaacgcgc
       61 cgggctcatt caaaaactgg cgcgcggtct gtgcccagtg atgcgccggt tgtttccggg
      121 cgtgcctaac gatcacccgg ctatcggctc gatgttgatg aacatggcgg cgaacatgct
      181 cgggcttggc aacgccgcca cgccgctcgg cctgcgggcg atgcgcgacc tcgagcgcct
      241 caatccgcat cccggcacgg cgacgaacgc gatgtgcacc tttctcgcga tcaacacgag
      301 ttcggtgcag ttgattccga ccaccgcgat cgcgatcctg gtcgccgcgg ggtcgaagaa
      361 tccgaccgcc atcgtgggaa cggccttgct cgcgaccttg tgtgcggcga cggtcgcgat
      421 cacctccgtc aagttgctcg agaagctgtc gtggttccgg gtccccgcgg agcgctttgc
      481 agctgggaag gatgatcccg gtgccgcgga gaggacaagc gatgactcga acgttcaacc
      541 cgcaaacctg ccgtcatccg gcgcggtcgg gatcaccgat cccgccgaca ccgggcacct
      601 acgaccgtgg gggaaagcgc tgctcgcgct cttcgcgctt ttctttcttg cgcttttcgt
      661 taggcagatt tttccggaga cgctcggttt gtcgcccgga acgaccgcac cggcgggcag
      721 ctacttcgtg cgcggtgtaa gcgcgctctc gctcctcgct atcccgttgc tgctttcgtt
      781 tttcgtgctt tatgccgcgc ttcgcggact caaggtctat gacgaattcg tcgagggggc
      841 gaaggaaggg ttcggcgtca ttcttaagat cattccgttt ctcgttacca tgctggtggc
      901 gattgggatg tttaaaggcg ccgggggcat cgagctgctg gcccgggcgc ttgcgcccat
      961 tctcacgccg ctccatttcc cgaccgatct gctgccgatc gcgctaatgc gtccgctcag
     1021 cggtggcgcg acgctcgcgc ttttcaccga cgtggtgcat cgactcgggc cggacaatat
     1081 cgtgagtctg atggcggcga cgatctacgg cagcacggag acgacgtttt acgtggcggc
     1141 ggtttacttc ggctcggtcg gcatcaagca gacgcggcac gccatcccgg caggcttgct
     1201 ggcggatttc gcgggggttg tcgcatcggt catcatctgc cgggcggtgt tatcgtgaat
     1261 cggtagatcc gaaaagcgcg gccccagcat cccgaggcga cgattcacaa tttatcgatc
     1321 catcttcctt ccactaactt ttccctcgcc atgccgttgt cacattctct atcgtccgat
     1381 tttcccaaat gattcaacgc gactatctca tcatcggcag cggcatcggc ggcagcagcg
     1441 tctgcgaagg catccgccag ttcgacaaaa aaggctccgt cacactcgtc ggcggcgaaa
     1501 cgtattatcc ctacaagcgc tggatgctct cgaagcaatt cctccgtgaa aagacgccgg
     1561 ccctgaaaaa actgccctgc ctcgaggagc gctggttcga ttcccacaag atcgagacgc
     1621 gcttcggcac ggtcgtcacc cagttcaaca tcgaccgccg gcttgcagtg ctcggcaacg
     1681 gcgaaacgat tgagttcaag aaggcctgcc tcgcgatggg cagccggccg gtccggccga
     1741 tggtcgcggg catcgggctg ggcaatgtga tttatctgcg cacggtccgc gacgcgctcg
     1801 ccctgcgcga gatggcgcag tcggaaaaaa tgatcgtgat tgtgggcggc ggattgctgg
     1861 cctgcgaagt ggcggccagt ctgcgccagg caaagctgaa gattgcgatc atgcatcggc
     1921 atcaatcgct tctgaatcgt tatctcgacc cggaaacgtc gacctgggta accgagcaat
     1981 ttgcgaaaaa gggcgtcacg ctgcttctcg gctcgagtct gaacgggttc gaaggcaaga
     2041 ccgtgctgcg gaatattcaa accaagagcg gcgagcgggt ggcggcgggc gtcgcggtcg
     2101 tcgcatgtgg gtcggaaccg aacctcgaga tcgtgcggaa cacgccgctc tcgggtccgc
     2161 acggatcgcc cgtgaccgat tacctcgaaa cggaagagaa ggggatttac gcagtgggcg
     2221 atctcgcctt ttatcccgac cgtttgatgg gtgggatgcg acggcaaacc cattgggaaa
     2281 atgcgcgcga gcaaggtctc atcgtgggcg caaatatcac cgggaaaaaa cggcaacgtt
     2341 atgagcagat tccgtatttc tggacggaaa tgttcgacct gaaatttgat ttcatcggtg
     2401 acttcaacgt gccggcgacc cacgtgcaac tgagtggttc ctatgcgaaa ggccgtttcg
     2461 cgctgcgcta tttccagggg gaaaaattgc gcgggatcgt gctctgccac cacagcgcgg
     2521 gcgaggtcaa ttcggcgaga aatgagctgc ggcaggggct cggcaaatag gcgcggacgg
     2581 cttaataaat ttggccggat attttcagcg caggattgcg cacccgaatg agcttccaat
     2641 cccacggcgc ccaactttcg cggcgccaat cgagttcaaa cggttccgtt aggctgttga
     2701 cccgggcgac gacctccggg acaaattctc cgcccgtgcc gccgatctga attttcgcgc
     2761 tccagtttcc cgtgggcggg tcgaggcgca cttgcggatc ggcggctgtg atcgtgagcg
     2821 acgcaaagga acgcaagacg aggcgcagac gattgagcac gcgcgtgcgg tcgtcgtccc
     2881 aatcgtcgcg gtaatcgcct gccataaaat caccggcccc cgaccagttc cgcgcttcga
     2941 ttttccggag gagatgctcg ctatggaggc ggacctgatt ttcctcctgc caaagttgcg
     3001 caagccaaag gccgagcgcc gctgcgacca cgattccggc gtataggcca atcctaaagg
     3061 ttgactgcac ggctcaccat aattgtgtcc gagcgattgc gccgccgcga tattcattga
     3121 cgggttaggc cgactccggc atgaaacgtg cgcacatccg gttgctcacg cttatgctct
     3181 ctcttggctc attcttcatt attttctgcg ctttgatcgt ttccgtctgg tgggcctgcg
     3241 ctgaagacaa gaaccaaact taaaggagaa ccgcccacga tgctcgatca cgatgacttg
     3301 aaatgggtcg cgaccaacgc gaccgaactc aaccgcctca tgcagcaggt gacgcgactc
     3361 tccgagcagg cccgccagca caaaggcgaa gggaattatt tggatctgct caacgagcag
     3421 gcccaacaag catgcaagac ttcgcaagcg ctcttcgacc gcatcacctc gcgcattctt
     3481 tatcgagccg ggtccgagat gcagacggcg gagccatctc ccagcctgcc cgggacggcc
     3541 gctccgtctg cacggacgaa tgtctccgaa agaatcccgc tcggggaaat gaaggtcgaa
     3601 acccagtcgg gtgatcctcc gatccgaaac cccaagggca aacgtgaact cgttcttgtc
     3661 gtggacgatg acctggatct cctcaaagtc gccgccgaca tgctcgagtt cgaggactat
     3721 cgcgtcatcc tggcgaaaga tggcctcgaa gccctgcaaa tttatcgacg gatgggcaaa
     3781 caaatagggc tcattgtgct cgactatttt ctcccggtga tggatggcga cgcggtcttt
     3841 gacgaactca aggcgatcga tcccgatgtg cgggtcgtcc tcagcagcgg ctttggcgaa
     3901 caggccaaac tgggcagcat gctcgcgcgt ggtctgtgcg gtttcctgcc caagccctac
     3961 acccacgaaa agctgatcgg acatattcag tcaatcatcg cggcctaacg agcgcaggcc
     4021 tttttgaggt cgcaggttgc tttccgcgca tcattccccg cgaattcgcg ttcgctggca
     4081 tcaatttagc tttcatggag ggcacgctcc atggcccgca tcgacgcatt cttcaagctc
     4141 atgtccgagg aaaaagcctc ggatcttcac cttgcgaccg gcaacccgcc gatgctgcgc
     4201 atcaacggcg aattggtgcg ggtcgattat cctccgctgc agaacgacga actcaaagcg
     4261 atggtctacg agatcgcgcc ggaagtgaag atcaaagtct tcgaggaaac ctccgatgtc
     4321 gatttcggct atgaagtccc gggcgtggcg cgttaccgcg ccaacttctt ccagcagaag
     4381 tccggcatct ccgccgtctt ccgcctgatc ccgtcgaaga tcttgacggt cgaggaactg
     4441 gggctcccgc cggttctgaa aaagtttccg cttctgaaaa aaggcctcat tctcgtcacc
     4501 ggcccgaccg gttccgggaa gtcgacgacg ctcgccgcga tgatggacta cgccaacctc
     4561 cagcggcacg accacatcat cacggtcgaa gatccgatcg agttcgtcca ccgcagccag
     4621 aactgcctcg tgcagcatcg cgaggtcggc gtccacaccc gctcttttgc ctccgctctg
     4681 cgcggagcgc tgcgcgagga tccggacgtt ctgctggtgg gagaaatgcg cgaccgcgag
     4741 acgatcgagt tggcgctgac agcggccgcg accggtcacc ttgtcttcgg gacgttgcac
     4801 acttcgagtg cgcccaaagc cgttgatcgc gtgatcgatg ttttcccgac cgaccagcaa
     4861 aaccagattc gcaccacgct cgcggaatcg ctcaaaggcg tcattgccca aaacctgttc
     4921 aagcgtatcg acaaacccgg ccgtgtcgcc gcgctcgaga ttctcgtcgt cgatggcgcg
     4981 atcgcaaacc tcatccgcga ggcaaagacc caccagatcc ccggtatgat ccaagtcggc
     5041 aagaagaagg gcaaccaacc gctcgacgac gcgatcattg agcaccttcg gcagatgcgc
     5101 atttcgccgg aggaagctta cgacaaggcg atcgatcgca aaaaatttcg cccctttctc
     5161 aagacaccgc cggcggacga gtttgaagat tagaactttc cgccatgcgc gtgcctgatc
     5221 tcgaccggat tctctcggcg atgctgaaga cctatgacgg catttccgat ctcaattttt
     5281 ccgtcggcca tccgttgcag gtcgaagatt atggcgaact aaagccggtc ttcattgatc
     5341 ctccgatcga gcgcctgacg acctaccaaa ccgaacaatt ggcccttgtc ctcatgcagg
     5401 gagaccgccg cttaatgcgc gactacctgg tagaaggctc gtgcgactgc agttattccc
     5461 tcggcggaga agcgcgcttt cgcgtcaata ttttccggca gcagggacat ttcgcgatcg
     5521 tgatgcggaa actggaggca gtcgtcccgt cgtttcgcac tcttgggctg cccttgatct
     5581 tcgaggaaat cgcgaaggaa aaagcgggtc tcgtgctcgt gaccggcgcc accggcagcg
     5641 gtaagacaac gaccctggcg gcgatcttga acgagattaa tgaaaagcta tcggtccatg
     5701 tcgtgaccct cgaggatccg atcgaattca ttcacccgcc ggcgcgcgcg acctttaacc
     5761 agcgcgagct cggccacgat ttcgacaatt atccgaacgg cttgcgcgcc gctctccgcc
     5821 agtcgccgaa ggtggtgttt gtcggtgaaa tgcgcgatcg cgccacggtc gaagttgcgc
     5881 tcatggcggc cgaaaccggc cacctcgtgc tgagcactct gcacacggtg gacgccggcc
     5941 agtcgatcaa ccgcattctc ggcctttttt cgttaggcga agagcagcaa ctgcgcattc
     6001 gtctggccga cagcctccgc tatgtcgtga gccagcgcct cgcgcccaaa atcggtggtg
     6061 gccggtatct cctgacggag attatggggc acaacctgcg cacccgcgaa acgatcgcgc
     6121 tcggcgaagc cgataatcgc agcttctacg aaatcatcga agcgagctcg cccttcggct
     6181 ggaccacctt cgaccgctcc gtcctgagtg cctacgaagc gggcgtcatc agcgaagaaa
     6241 cggcccgcgg gttttcctcg aaaaagggga aggtgatccg cggaatcgac ctgatccaga
     6301 agggacgcgg gatcgagaac gaaaccgaag gcgggctgcg actcgatctg ccggtcaatg
     6361 cttttcgtta ggaggcaaac atgggaagcg atccagacag taattttttt gacctcggcg
     6421 acaagaccgc cctcgtctgc attgaccatc cgcagtacca gaacatgatc gtgccgcagt
     6481 tgaccgagct aaattataag gtgcatctcg gcctcttcga agaagatgtc ctcctgaaac
     6541 ttgccactta cagctacaat gtggtcgtcg tttacgagaa ctttaagcgc tcgaaagtcg
     6601 atgataatcc aatcctgcgc gagatggtga agcggaccaa ctcgctccgg cgcgagcatt
     6661 ttgtcatcct cctcagccat cgcttcgcga cgaacgacgc gatgagcgct ttcgtccaga
     6721 gcgtcgatca gattatcaac atcactgaca tcgcgaattt caagccgatc atgcggcggg
     6781 gcgtagcgca gcagcgcgaa ctctacaccc cgtttcaaga gacactcaag gccgtgcggg
     6841 cgtcctagcg cgcggaaatt tccagcgccc gcagcgcgtg gcgcgatttt ttggaaacgt
     6901 cttgcgggtg cgcgatcccg cgtggtaatt tccgggcgcg ccgacatgaa gctcgttcaa
     6961 tgggttcgat tcacctggga tttagacacg ctttcactcc tcggcgcggt cctcccggag
     7021 cattacaact tcgtcgttgc gaccgaggct gacgagaagg aactgcgcgc cgtcattacc
     7081 cgctcctttg cgcacgacac ctcctggggc gacgccatcc acgaggtcaa cgacatgatc
     7141 gacggctggc tgctgcgggc ttttgcgccg ggcgatggcg gcggggtctg cctggcgttg
     7201 cgccacggct tgcgcatgat cggcgcatca attttgatcc ccgcgccggc ggccgaaaac
     7261 catctttccc ccggaccctg cgtgctgatg gagtatcgca accgcggcct cggtaccgcc
     7321 ctgctcgggg agtcgctcca ccgcttgcgc gaggtggggc tggcccgggc caccgcccgc
     7381 acgaaacgga acgcgcccgt tgccaaattc ctttacccga agttcaacgg gattctgacc
     7441 ccgggggaca cgccacttct cgccgcctga tcgttcggcg caaggcggcg gggcggcgga
     7501 cctcagcgaa taatgccgcg ctcgctcgcc gcgatgaatt gaagaatgga gcgcacatct
     7561 tcgctttccc ccagctgctc gcgcaccgtt tccagcgcct gaccggtgtt catcttcgcc
     7621 ctataaatca aacgaaacgc ttccttgatc ggcttgatct tttccggcgg ataaccgtgg
     7681 cgctccagac ccacctgatt gatcccgcgc acttccgccg ggttgccatc ggcgatcatg
     7741 aacggcggca catcctgcac aatcttggag cagccgccgg tgatcgcgaa acggccgatc
     7801 cggcaaaatt gatgaaccgc cgtcaaaccg ccgatgaccg cgtggtcgcc cacctgcaca
     7861 tggccggcga gcgtgccgtt gttggagaaa atgacggaat cgccaaccgt gcaatcgtgc
     7921 ccgatgtggg agtaggcgag gaaatttccg ccgctcccga cgcgcgtctt gccgctctcc
     7981 aaagtgctgc gattgatggt gacgaactcg cggaagcaat tttcgttacc gatctcgaga
     8041 taagtgggct cgccgcgata tttcaaatcc tgagtttgct gcccgatcga gcagtaggcg
     8101 tagaatttat tcccccggcc ggtggcgagc ggtcccccga gcgtgacatg atgctgcaac
     8161 cagcaatttt cccctaacga caccccggcc gcgacgatgc agtaaggacc gatgaccgtc
     8221 ccggtgccga gctgcgccga cggatcgaca atcgcggtcg gatgaatctg ggtctcagac
     8281 atcgtcggaa tgacgaatga cgaatgacga agggcgaagg aatgaccaat gacaaatgcg
     8341 gcagtcgacg cccggcgcgg cggctggctg tctcttcatt cggtattcgt cattccttcg
     8401 atcttcggaa tccgtcattc gtcatttccc cctacttatc caaaaacgtg aacatcagtt
     8461 caccctcgga aacgaccgcg tcattcacca cacaccggca catcgctttc gcgagacggt
     8521 tgccgcggga cttcgtcagc tctgcgtgaa tgaagagggt gtcgccagga aaaaccggtt
     8581 tacggaattt cacgccgtcc gcgctcatga agtaaccgac ccggctcgcg ctcttgctga
     8641 tccgcatcat cagcacgctc gccacctgcg ccatcgcctc cacctgcagc acaccgggca
     8701 tcaccggatg acccgggaaa tg
//
LOCUS       DATMNS010000076         2436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3271768, whole genome shotgun sequence.
ACCESSION   DATMNS010000076 DATMNS010000000
VERSION     DATMNS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3271768"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VNW28_02855"
     CDS             complement(<1..352)
                     /locus_tag="VNW28_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08893.1"
                     /translation="MEPGIYSQSKARRGDLAILLAVAAARLVLHCATNWQYGFHRDEL
                     GVLDDARYLAWGYVSYPPFTPFIARVALILFGPSLVGVRLFAALAQSVAMVFAGLMTR
                     ELDGRRWAQVVAALA"
     gene            complement(535..1341)
                     /locus_tag="VNW28_02860"
     CDS             complement(535..1341)
                     /locus_tag="VNW28_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08894.1"
                     /translation="MFPLQTNSLPATADALCAALEQSLRRIVRPAGPMVTVEEKQYPE
                     LAAIRVSLDGADAGDRPPPRPLPPVGAIEPGLRVENFEIVGRPILVGRGKINLVCSAR
                     DVRLGQGRDGEGNVLLLLQNAAEGNVEVDVALDDLEALLLAGAKAEAAKQGVTVESVR
                     VELRSRSDHALDLVVQVRAKKLFLNAAVRISGSVAIDEQLNVRFSALECAGEGTLGAL
                     AGGFIAPHLQRFNGRAFSLMALSLGEVKLRDVRIAADRELRVTAQFGQAD"
     gene            complement(1476..2012)
                     /locus_tag="VNW28_02865"
     CDS             complement(1476..2012)
                     /locus_tag="VNW28_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017504874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HXA08895.1"
                     /translation="MKRNNSKKTQELKVRRSAPLATRTDLTPSATKDIAGAMNAILAD
                     VFALYLKTKNFHWHMSGPHFRDYHLLLDEHADQLFAMTDPVAERVRKIGGPTLKSIGQ
                     ISRTQRVVDNNAEYVEPLDMLAELADDNRTLAARLRETHNVCDEHRDFATASLIEVWI
                     DETERRTWFLFEATRRRN"
BASE COUNT          456 a          790 c          707 g          483 t
ORIGIN      
        1 tcgcgagcgc ggcgaccact tgcgcccagc ggcgtccgtc gagttcccgg gtcatcaagc
       61 cggcgaagac catcgcgacg ctttgcgcga gcgcggcgaa gaggcgcacc ccgacgagcg
      121 agggtccaaa aagaatcaat gcgacgcgtg cgatgaacgg cgtgaaaggc ggatacgaaa
      181 catagcccca ggcgagatag cgcgcgtcat cgagcacgcc gagttcatcg cgatgaaatc
      241 cgtattgcca gttggtcgcg caatgcagaa ccaaacgcgc cgccgccacc gccagcagga
      301 tcgcgagatc gccccgtcgg gctttcgatt gcgaataaat tccgggctcc atctgcggcc
      361 tctgcaggct atcgattcag cgcacatcgc acaacgcctg taaggcatgc gcttgctcgc
      421 gaaacccttc ggagctctgc ctgccgctgt acccgtggag ggacgggctc tgtcccgtcc
      481 gtgtttcttt tgccggggaa cgaatctcgg acgacacgga ggtcgtccct ccagttagtc
      541 cgcctggcca aactgcgccg tcacccgcag ctcgcggtct gccgcgatcc gcacgtcgcg
      601 taacttcact tcgcctaacg acaacgccat gagcgaaaac gcgcgcccgt tgaaccgctg
      661 cagatgcggc gcaatgaatc caccggcgag cgctccgagc gttccttccc cggcgcattc
      721 caaggcagag aaccgcacgt tcagttgctc atcgatcgcc acgctgccgc tgatccgcac
      781 cgccgcgttg agaaaaagct tcttcgcgcg cacctgcacg accagatcga gcgcgtggtc
      841 cgagcgcgat cgcaactcga cccgcacact ttccaccgtc acaccctgct tcgcggcctc
      901 tgccttcgcg cccgcgagca acagcgcctc gagatcgtcg agcgcaacgt cgacctccac
      961 gttcccttcc gccgcattct gcaaaagcag gagcacattg ccttcgccgt cccgtccctg
     1021 gccgaggcgc acgtcgcgcg cgctacagac aagattgatc ttccctcgcc ccaccaggat
     1081 cggtctcccg acgatttcga aattctctac ccgcaagcct ggctcaatcg ctccgaccgg
     1141 cggcagaggc cgcggcggcg gacggtcccc ggcatccgcg ccatcgagcg agacccggat
     1201 cgccgccaac tccggatact gcttttcctc caccgtcacc atcggtcccg ctggcctaac
     1261 gattcggcgc aggctttgct ccagcgccgc gcagagcgcg tcagcggttg ctggcagcga
     1321 attggtttgc agcggaaaca tcccgccaac ctacgcaatt tggctcgcga gcggatgttc
     1381 tttccgggca gctcacagcc tgcccgcccg tgtgccggcg cctcgccgaa gcaggcgcgc
     1441 aacaagatgg tgtttctctt tttcggtcga aagatttaat tgcgtcgccg ggtcgcctca
     1501 aacaaaaacc acgtccggcg ttcggtctcg tcgatccaaa cctcgatcaa gctcgcagtc
     1561 gcgaagtcgc gatgttcgtc gcagacgttg tgcgtctcgc gtaagcgcgc cgccagcgtc
     1621 cggttgtcgt ccgctaattc tgccagcatg tcgaggggct caacgtactc ggcattgtta
     1681 tcgaccacgc gctgggtgcg agagatttgg ccgatcgatt tgagggtcgg tccgcctatc
     1741 ttgcggaccc gttccgcgac tggatcagtc atcgcgaaaa gctgatcggc atgttcgtcc
     1801 agcagcagat ggtagtcgcg gaaatgcggg ccgctcatgt gccagtggaa attcttcgtt
     1861 ttcaggtaaa gcgcgaagac gtcggccagg atcgcattca tcgctcccgc gatatctttc
     1921 gtggcggaag gcgtgagatc ggtgcgggtg gcgaggggcg cgctacgcct caccttgagt
     1981 tcctgggtct ttttcgaatt gtttctcttc atggattttt gctctctggt gttggttgat
     2041 ggttggtccc ggctcaccac tgcaagtgga accttaagac tgattcgccg atgctccgtc
     2101 taccggatgc agccacgggg ccgcttccct cttgcgcagt ctttgggcgt gttcctgcgg
     2161 accgtcggtg atccttcatt gtcccataat tactagcggc ggccggaacg cgtcaacgga
     2221 ggataatctg cgtaacgata tgctgctggc agttccagtc gaaggcgtga atctgaaact
     2281 gaaaggctcg cgcgacatcg gagtcatccg cgcgcggcat agagatagat tgcgaccaga
     2341 acgacgagca caagaatggc cgtcacccaa caaacggttt tgatgacggc tggtttgggt
     2401 ggtgatccga actcctgcgc gacgaactca tcatag
//
LOCUS       DATMNS010000077         4449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_818285, whole genome shotgun sequence.
ACCESSION   DATMNS010000077 DATMNS010000000
VERSION     DATMNS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818285"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1449
                     /locus_tag="VNW28_02870"
     CDS             <1..1449
                     /locus_tag="VNW28_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08896.1"
                     /translation="LMPQTDNHLAVFLGHVQANPQVKALLGAFGGTTGLVATLLALRS
                     KFSRALGATRGAQWILVLVAILFFVLLSLVISWFLLFLGSQSWADKAGRWFLGQGETY
                     QTQLFVVFFLTGGNLLFGIVMGFFINVNKFSLHATYRMRLIRAFLAASRTQDMRRPHL
                     FTGFDPNDNFPLRDLRPGKPFHVINTTLNLVKGTPLAWQERKAESFTMSRLHCGSWHV
                     GYRPSADYGDGVSLGTALTISGAAANPNMGYHSSPLVGFLMTLFNVRLGWWLGNPGTA
                     GERTWRRAGPRYAVGPLFSEALGNTTDCYKYVNLSDGGHFDNLGLYEMVLRRCKLILA
                     VDAGQDFDYVFEDLGNAIRKIRIDLGVPIDIRVVSPKKADGTMHRCAFGTIQYSVIDD
                     SQSDGFLLYIKPMIFGDEPPDVSNYAAAHPEFPHEPTTDQWFSESQLESYRLLGLHAI
                     ESICGGECEPKNLPLFFAQVKDDLARAKTGAA"
     gene            1540..3705
                     /locus_tag="VNW28_02875"
     CDS             1540..3705
                     /locus_tag="VNW28_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08897.1"
                     /translation="MKPHALERGWAGRPLASVATCALVALSLLAAGCGKAPEDEAKLA
                     GLSPNDFPQITADIFQPMDGGLALAPNEIMGRNTWDLWSGGNQHFWNHVAQDSFGLMD
                     LLKTLDNRKYPRSERFKTLGLVNEPGFRAATKPDEYGLWLDEQVDPEPAGIDYKIYGK
                     PSGVLGFRLFPNPEFNAEARQKWDGARFMNDPHYYSTNQLIRPYRVGVACGACHISPN
                     PSNPPADPENPRWENLASAIGNQYIREGKVFAPNVEKGGFFYEELAAQPPGTSDTSRI
                     ATDHINNPNAINPIFLLAERERIAVPEKMAGGTLALPGEKEEMAVPHILKDGADSIGV
                     PGATIRVYVNIGMFSEYWLTRHNRLIGLTPQKPFEISYAREHSVFWRATEERLANVAA
                     FFRRLKPYHLADAPGGAAYLTTDEAVLNRGKLVFANSCAYCHSSKQPPAGVDPQSGDG
                     EAWFRSAVTQPDFLENNFLSNDRRYPLTKIQTNSARAFGTNALAGHVWDNFSSQTYKE
                     LPLPGEVEFYNPFDETQPIKFEPNAKHTGPGYYRVASLVSLWSSAPFLHNNALGKFTG
                     DPSVAGRLEAYNDAAEKLLWPEKRLGKASIWRTQNECYLHLRKEFVPKPLQSLADNDG
                     YLKLGPVPKGTPLNLLANLEPDFDDLVALQLKLSKALLAIHAQNLSPEEARAELIKTV
                     PQLLSANKCPDFIEDEGHYFGTDLPDDDKRALIEYMKTF"
     gene            3780..4196
                     /locus_tag="VNW28_02880"
     CDS             3780..4196
                     /locus_tag="VNW28_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08898.1"
                     /translation="MSQAELDDLFTKSPAGEIPKGEAKGTAIVAPGTTYSENIATFVN
                     HFAWQGKVFDPEKGVLRNKITPFGLNAIIAKVYKGESWLDGQECIVLDYSETSLLAHW
                     IRDEIREIEPRVYLGKVYWSKKRLIDFALEFPAAST"
     gene            4213..>4449
                     /locus_tag="VNW28_02885"
     CDS             4213..>4449
                     /locus_tag="VNW28_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08899.1"
                     /translation="MADLSEAGPSQPPAFNSNASRPLPRADTGSVFLNGLSDFFTLLL
                     HLERRVDPWFRPAFDALLRDPIARFTTDLINRRRP"
BASE COUNT          883 a         1472 c         1218 g          876 t
ORIGIN      
        1 ttgatgcccc agacggataa ccacctcgct gtcttcctcg gccatgtgca ggcgaatccc
       61 caggtcaaag ccctgctcgg agcgttcgga ggaacgacgg gcctcgtcgc caccttgctc
      121 gcgctgcgct cgaaattcag ccgggcactc ggcgcgaccc gcggcgcgca gtggattctg
      181 gtgctcgtgg caatcctctt tttcgtcctc ctctcgctcg tcatctcctg gttcctgctc
      241 ttcctgggct cacaatcctg ggcagataaa gccggccgat ggttcctggg tcagggtgag
      301 acttatcaga cccagttgtt cgttgtcttc ttcctcaccg gcggcaatct cctcttcggc
      361 atcgtgatgg gtttcttcat caacgtaaac aaattctccc tccatgccac ctatcggatg
      421 cgattgattc gcgcctttct cgcagcttcg cggacacagg atatgcgccg gccgcatctc
      481 tttaccggct ttgaccctaa cgacaatttc ccgctgcgcg acctgagacc gggaaaaccc
      541 tttcatgtca tcaatacgac gctcaatctg gtcaagggca cgcccttggc gtggcaggag
      601 cggaaagccg aatcctttac catgagccgg ttgcattgcg gttcgtggca tgtcggttac
      661 cggccttcag cggactacgg cgacggagtc agccttggca ctgcgctcac catttccggc
      721 gccgccgcca atcccaacat gggttatcac tcctcgccgc tcgtcggttt tctgatgact
      781 ctgtttaacg tgcgcctcgg ctggtggctg gggaatcccg gcacggccgg cgaacggacc
      841 tggcgccggg ccgggccgcg ctatgccgtc gggccgctct tttccgaggc gcttgggaat
      901 accacggact gctacaaata tgtcaatctc tccgatggcg ggcacttcga taatctcggc
      961 ctttacgaaa tggtattgcg ccgctgcaaa ctcatcctcg cggtcgatgc cgggcaggat
     1021 ttcgattacg tctttgaaga tctcggcaac gccatccgca aaatccgaat cgacctcggc
     1081 gttccgatcg acatcagggt cgtctcgccg aagaaagccg acggcaccat gcatcgctgc
     1141 gcttttggca cgatccaata ctccgtcatc gacgactcgc agagcgacgg cttcctcctt
     1201 tatatcaagc cgatgatctt cggcgacgag ccgcccgatg tctccaatta cgccgcggcc
     1261 catccggaat ttccgcacga accgacgacc gaccagtggt tcagtgagtc gcaactggag
     1321 agttatcgac tgctcgggct gcacgccatc gagtcgatct gcggagggga gtgcgagccg
     1381 aaaaacctgc cgctcttctt cgcccaggtg aaagacgatc tggcgcgggc aaaaaccgga
     1441 gcggcctgag ccccgatcgc tgccgcgcac cgccggcgtg gaaattttcg cttccaattc
     1501 tgcttgctcc ccgggtcgga agaggattaa aaacctgtta tgaaaccgca cgcgctggaa
     1561 cgcggttggg cggggcggcc cctcgcgtca gttgccacct gcgccctcgt ggcgctctct
     1621 ctcctcgccg ccggctgtgg caaggctccg gaggacgaag ccaagctggc cgggctttcg
     1681 cctaacgact tcccgcagat cacggccgac atttttcaac cgatggatgg cgggctcgcg
     1741 ctcgcgccta acgagatcat gggccgcaac acctgggacc tgtggagtgg cggcaatcag
     1801 catttctgga atcacgtcgc gcaggacagt ttcggtttga tggacctgct caagacgctc
     1861 gacaaccgca agtatccccg cagcgagcgc tttaagacgc tcggcctggt caacgaaccc
     1921 ggcttccgcg ccgcgaccaa gcccgatgaa tacggcctct ggctggacga gcaggtcgac
     1981 cccgagccgg ccgggatcga ctacaaaatc tacggcaaac cctccggcgt cctcggtttc
     2041 cgtctcttcc cgaaccctga gttcaacgcc gaggcgcgcc agaaatggga cggcgctcgt
     2101 ttcatgaacg acccgcacta ttacagcacg aaccaactca tcaggccgta ccgggtcggc
     2161 gtcgcctgtg gcgcttgcca tatctcgcct aatccatcca acccgccagc cgacccggaa
     2221 aatccgcgct gggaaaacct cgcctccgcg atcggcaacc aatacatccg ggagggcaaa
     2281 gtctttgcgc cgaacgtcga gaagggcggc ttcttttacg aggaattggc cgcgcaacca
     2341 cccggcactt ccgacacctc gcgcatcgcg accgatcaca tcaacaatcc caacgcgatc
     2401 aatcccatct tcctcctcgc cgaacgcgaa cgcatcgcgg tgccggaaaa aatggccggc
     2461 ggaacgctcg ctttaccggg tgaaaaagaa gaaatggcgg tcccccacat cctgaaggat
     2521 ggcgcggatt cgatcggcgt ccccggcgcg acgatccgcg tttacgtcaa catcggaatg
     2581 ttttcggaat attggctgac tcgccataat cggctcatcg gcctgactcc gcagaagccc
     2641 tttgagattt cctacgcgcg cgagcactcc gtcttttggc gtgcgaccga ggaacgtctg
     2701 gccaacgtcg ccgccttttt ccgccgcctc aagccctatc atctcgcgga tgcgccgggc
     2761 ggcgcggcct acctcacgac cgacgaagcg gtcttgaacc gcggcaaact ggtctttgcc
     2821 aatagttgcg cctactgcca ctcgagtaag cagccgccgg cgggagtgga tccgcaatcg
     2881 ggcgatggcg aagcctggtt ccgcagcgcc gtcacgcaac cggatttcct cgagaacaac
     2941 tttctctcca acgaccgacg ttatcccctg acgaaaatcc agacgaactc cgcgcgcgcc
     3001 ttcgggacca acgcgctggc ggggcacgtc tgggacaact tctcttccca aacctacaag
     3061 gagttgccgt tgcccggcga ggtcgagttt tacaatccgt tcgacgaaac ccagccgatc
     3121 aagtttgaac cgaatgcgaa gcataccgga cccggttatt accgggtggc ttcactcgtt
     3181 agtctgtgga gttcggcgcc gttcctgcac aacaatgcgc tgggcaagtt caccggcgac
     3241 ccctcggtcg cgggccggct cgaggcctac aacgacgccg ccgagaaact gctctggccg
     3301 gagaaacgcc tcggcaaggc ttccatctgg cgcacccaga acgaatgcta cctgcacctc
     3361 cggaaggaat ttgtcccgaa accgctccag tcattggcgg acaacgacgg ttacctcaaa
     3421 ctcggccccg tccccaaggg cacaccgctc aacttgctgg ccaatctcga gcccgacttc
     3481 gacgacctgg tcgcgctcca gttgaaactg agcaaggcgc tgctcgcgat tcacgcgcag
     3541 aatctctcgc cggaagaagc ccgcgccgag ctcatcaaaa ccgttccgca attgctctcg
     3601 gccaataaat gccccgattt catcgaggac gagggtcact atttcgggac cgatctgccc
     3661 gacgacgata agcgggcgct gatcgaatac atgaagactt tctagcgcaa catcatcccg
     3721 agcgccgctc gggaatgacc cgacgaaagg acaccatgac cgttcctgaa ctactgaaaa
     3781 tgtcccaggc cgagctggac gatttgttca ccaagagtcc tgcgggcgag attccgaagg
     3841 gcgaagccaa gggcacggcg atcgtcgccc ccggcaccac ttacagcgaa aatattgcga
     3901 cctttgtcaa tcacttcgcc tggcagggca aagtcttcga cccggaaaag ggcgtgctcc
     3961 gcaacaagat cacccctttc ggcctgaacg cgatcatcgc caaggtttac aaaggcgaga
     4021 gctggctcga cggccaggag tgcatcgtgc tggattactc cgagacctcg cttttggcac
     4081 actggattcg cgatgaaatt cgtgagatcg aaccgcgcgt ctacctcggc aaagtctact
     4141 ggagcaagaa gcggctgatc gatttcgcgc tcgaattccc ggccgcgtcg acctaacgag
     4201 aagctgacgg ccatggcgga cctttccgaa gccggcccca gtcagccgcc ggcttttaat
     4261 tccaatgcca gtcgccccct gccgcgggcg gataccggct cggtcttcct caatgggttg
     4321 agcgatttct ttaccttgct cctgcacctg gaacgccggg tcgatccctg gtttcgcccg
     4381 gccttcgatg ctctcttgcg cgacccgatc gcccgcttca caaccgacct gatcaatcgc
     4441 cgccgccct
//
LOCUS       DATMNS010000078         6766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5112903, whole genome shotgun sequence.
ACCESSION   DATMNS010000078 DATMNS010000000
VERSION     DATMNS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5112903"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /gene="nuoL"
                     /locus_tag="VNW28_02890"
     CDS             complement(<1..1436)
                     /gene="nuoL"
                     /locus_tag="VNW28_02890"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXA08900.1"
                     /translation="MTSLLPWIALLLPLASVFLILLATRPLRTLSAFISVAAVLASFV
                     CSCLIFFQQTSTAPTISWLDLAPLFSVPLGLTLDPLSRSMLFMVTGVGAVIHVYSLGY
                     MSADEGKSRYFAGLSLFMFAMTGIVLANNFVMMFIFWELVGVSSYLLIGHWFERPAAA
                     EAAKKAFITTRIGDFGFMLGILMVWTATGSLVFTEIAAGMAKFATHPGYLTAAALLVF
                     CGAVGKSAQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVYMLVRVGFLIDASLGALQ
                     VIAWIGTITAVLAALMATQQNDIKRILAYSTLSQLGYMVMAVGLASGQAAMFHLFTHA
                     AFKALLFLGAGSIIYMLHHEQDIWRMGGLAKRLPITFLTFLVGTLALIGFPGFSGFFS
                     KDAILALAYERDLPIFVFAAFTALLTAFYMTRLMVVAFFGNARSPHAARGAESPLVMT
                     LPLILLSVPAAIGGFGFFARNFLSLPNES"
     gene            complement(1677..1997)
                     /gene="nuoK"
                     /locus_tag="VNW28_02895"
     CDS             complement(1677..1997)
                     /gene="nuoK"
                     /locus_tag="VNW28_02895"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXA08901.1"
                     /translation="MVTLGDYLLVSGLLFTLGFAGVLLRRNLIIIFMSLELMLNAANL
                     SLVAFSRFRTNSLGQPNYDGQVFVFFIITVAAAEVAVGLAIIVALYRARRTTHVEDIN
                     TLKF"
     gene            complement(2002..2505)
                     /locus_tag="VNW28_02900"
     CDS             complement(2002..2505)
                     /locus_tag="VNW28_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXA08902.1"
                     /translation="MSPIFFWTAAALMLVFGAAVIINRNPVASALSLVVSFLGLAALF
                     MSLDAFFIGIIQVLVYAGAVMVLFLFIIMLLDLRAGERRRINFVASAGGAAVAVIFFG
                     QLYLVIGHLHQARLPFAPLAPEQVDDVHRVGLLLFGNYNLPFQIIGVLILVATIGVIV
                     LSKRELH"
     gene            complement(2609..3139)
                     /locus_tag="VNW28_02905"
     CDS             complement(2609..3139)
                     /locus_tag="VNW28_02905"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HXA08903.1"
                     /translation="MPITVRRPNLSWSEKVYLPAIFSGMIITFRHLKNMLLGRTRVTM
                     QYPEQKWDSQMPDHYRGAPALVKDEGGRVRCVACQLCEFICPPRAIRIVPGEIPLGER
                     FAKVEKYPEEFDIDMIRCIYCGMCEEVCPEQAIFLRKDYAITGYTRAEMVHDKKKLIE
                     IGGVMHGLVLKWNERK"
     gene            complement(3296..4459)
                     /locus_tag="VNW28_02910"
     CDS             complement(3296..4459)
                     /locus_tag="VNW28_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HXA08904.1"
                     /translation="MNPTLFLILTSLLKILGIVFVVILPLVSYTVYAERRVSALIQDR
                     VGPNRVGFPVSLLGFKKDFSPFLGGLGQPIADAMKFILKEDFTPASVNKFYYWLAPCL
                     AMVPAIMTIAVVPFGSTLFGVPMVIADINVGVLYVFAIASLGVYGIVIAGWSSNSKYP
                     FLGGVRSSSQMISYELSLGLAVIPIFLLVGQLRLTNIVQYQIENGWMIAPFVGHWADG
                     WKWLLAIPMFISFVVFTIAIFAETNRLPFDLPEAETELVGGYHTEYGSMKFALFFLGE
                     YAAMITGSAVIVTLFLGGWHLPVPWWNQAHGFHWLLIDGSLGGWRGVIGGLFNIFVFF
                     GKVSVLLLFFIWVRWTLPRFRYDQLMRLGWVFFFEIALVNIFLAAIIIACLPK"
     gene            complement(4557..6266)
                     /locus_tag="VNW28_02915"
     CDS             complement(4557..6266)
                     /locus_tag="VNW28_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXA08905.1"
                     /translation="MVNVQIDGVWHQFPKGTRLIEACEQAGSYVPRYCYHKKLSSPGN
                     CRMCLIEMGMPKMGPDRKPILGADSHPEINWMPRPQISCATDVSEGLGVRTNSPLVEE
                     CRRGVMEFLLINHPLDCPICDQAGECQLQEFSVEYGRQESRFLENKVKKPKNVVLGPR
                     VTLDDERCILCSRCIRFCKEIAHDDVLGFVDRGSHTTLTAHPGKRLENNYSLNTVDIC
                     PVGALTSSDFRFKMRVWFLKETKSFCTSCATGCNTIIGTREDVIYRQTPRENDAVNSD
                     WMCDYGRLNFAYLENDRRLRAPMVRAEGKLTAATWRHAIASAAAQLKPYVGWQTAILA
                     SGRMTNEELWLAGRLAESLGATLIDIVPRAGESDDILLSADRNPNTNGARLLRLASRS
                     GTVLQQIKEAVWSGQIQALITLGENPLHCGITPEYLARLPVYVVLDLLENDATGHASV
                     VLPSLGFAEKRGSMINRRGRLQRLNRAVRGPAEARDDWEILRDLVQAISGGDSEGQAR
                     ARGLSTIEDVFRQMSEAVPEFSGLTLSRIGDLGVPVLHADGPPVDPARGEMRERELEK
                     QPQ"
     gene            complement(6332..6766)
                     /locus_tag="VNW28_02920"
     CDS             complement(6332..6766)
                     /locus_tag="VNW28_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXA08906.1"
                     /translation="MNQENRKAGKDLRSRGITQRVIGAAIAVHRDLGPGFLESIYEEA
                     LAVEFALEGIEFVRQKPVSLFYKDHQIGEHRLDFVVEDLIVLELKAIRALEDIHFAIG
                     RSYLKATGLHDGLLLNFATTPLTIKRFCRERPASSSNTELIL"
BASE COUNT         1538 a         1907 c         2153 g         1168 t
ORIGIN      
        1 gactcgttag gcaggctgag gaaattccgg gcaaaaaaac caaacccgcc gatcgccgcc
       61 gggacactga ggagaatgag ggggagcgtc atgacaaggg gagattctgc gccgcgggcg
      121 gcgtgcgggc tgcgcgcgtt gccgaaaaat gcgaccacca tcaagcgtgt catgtaaaag
      181 gcggtgagca gagcggtgaa agcggcaaat acgaagatcg gcaggtcgcg ttcgtaggcg
      241 agagcgagga tcgcgtcttt gctgaagaag ccgctgaagc cggggaaacc gatgagcgcg
      301 agcgtgccga cgagaaaagt gaggaacgtg atcggcaacc gctttgcgag tccgcccatg
      361 cgccagatgt cctgttcgtg atgcagcatg taaataatcg agccggcgcc gaggaagagg
      421 agcgccttga aggccgcgtg ggtgaagaga tgaaacatcg ccgcctggcc ggaagcgagg
      481 ccgaccgcca tcaccatgta gccgagctgc gaaagcgtgg agtaggcgag gatccgctta
      541 atgtcgttct gctgggtcgc catgagcgcc gcgaggacgg cggtgatcgt gccgatccag
      601 gcaatgacct ggagcgcgcc taacgacgcg tcgatcagga atccgacgcg caccagcatg
      661 taaacgccgg ccgcgaccat cgtcgcggca tggattaagg ccgagaccgg cgtcgggcct
      721 tccatcgcat ccggcaacca aacgtgcagt gggaattgcg ccgacttgcc gaccgcgccg
      781 caaaaaacga gcagcgccgc ggcggtcaaa tagccgggat gggtcgcgaa tttcgccatc
      841 ccggccgcga tctcggtgaa gaccaacgaa ccggttgcag tccagaccat caggatgccg
      901 agcatgaaac cgaagtcgcc gatgcgcgtc gtgatgaacg cctttttcgc ggcctcggcc
      961 gccgccgggc gctcgaacca atgcccgatg agcaaatacg aactgactcc gaccagttcc
     1021 caaaaaatga acatcatcac gaagttgttc gcgaggacga tcccggtcat ggcaaacatg
     1081 aaaagtgaga gcccggcgaa atagcgcgac ttgccttcgt ccgcgctcat gtagcctaac
     1141 gaatagacat ggatgacggc gcccacgccg gtgaccatga acagcatcga gcggctcaac
     1201 ggatcgaggg tcaggccgag cggcacggag aagaggggcg cgaggtcgag ccaggaaatc
     1261 gtgggcgcgg tggaagtttg ctggaagaag atgaggcagc tgcaaacaaa gctcgcgagg
     1321 acggccgcga cggaaatgaa agcgcttaag gtgcgcagtg ggcgggtcgc aaggaggatg
     1381 aggaagaccg aagcgagcgg caggagcagg gcgatccagg gcaggagcga agtcatcgcg
     1441 caatccttcc gtaaacgatt tcagttgtga cgccggcgat aagttctggg gagcgcacgc
     1501 gacatgcgtg ctggtggcgg cgactcgccg ccacggactt tttcctggag agcgtcgaac
     1561 gaccaacgag atcacgacca gcgcccaaag aaaagttcgc gctggcgagg tcgcaagcgc
     1621 cagcacgcga gtcgcgtgcg ctccccagag ttctgcgatc gcgcgcgttc ctcatttcaa
     1681 aacttcaagg tgttgatgtc ctcgacatgg gtcgtgcgcc gggcccggta aagcgcgacg
     1741 atgatcgcga gcccgaccgc cacctccgcc gccgccaccg tgatgatgaa gaaaacgaaa
     1801 acctgcccgt cgtaattggg ctggcctaac gagttggtgc ggaagcggga aaaggcgacg
     1861 agcgaaagat tggccgcgtt cagcatcagt tcgagcgaca tgaaaatgat gatgaggttg
     1921 cggcgcagga ggacgccggc gaaaccgagg gtgaaaagca acccactgac tagaagataa
     1981 tcgccgaggg tgaccatttc cctagtgcag ctcgcgtttg ctcagcacga tcacgccgat
     2041 ggtcgcgacg aggatgagca cgccgatgat ttggaagggc aggttgtagt tcccgaaaag
     2101 aagcaggccg acgcggtgca cgtcgtccac ttgctccggg gcgagcggag cgaacggcag
     2161 cctggcctgg tgcaagtgcc cgatcacgag gtagagctgc ccgaagaaaa tgacggccac
     2221 ggccgcgccg ccggcagagg ccacgaaatt gatccggcgc cgttcccccg cgcgcagatc
     2281 gagcagcatg atgatgaaga ggaacaggac catgaccgcg ccggcgtaaa cgagcacctg
     2341 gatgatgccg atgaaaaacg cgtcgagcga catgaagagc gcggccagcc cgagaaaaga
     2401 aacgaccagg ctgagggcgc ttgcgaccgg gttgcggtta atgatgaccg ccgcgccgaa
     2461 gacgagcatc agcgcggcgg cagtccagaa gaagatcggg gacataggaa atcctaatgg
     2521 tgtgatcgcg gagcggagtg atcgagtaat aggcgagaga tccccaacac ttcattactc
     2581 caacactcca ttaatccacg gccgcgcctc atttcctctc gttccatttc agcaccaggc
     2641 cgtgcatcac gccgccgatc tcgatcagtt ttttcttgtc gtgcaccatt tcggcgcggg
     2701 tgtagccggt gatcgcgtag tctttgcgca gaaaaatcgc ctgttcgggg cacacttcct
     2761 cgcacattcc gcagtagatg cagcggatca tgtcgatatc gaattcctcc ggatattttt
     2821 ccaccttggc gaaacgctcg cctaacggta tctcgcccgg cacgatccgg atcgcgcgcg
     2881 gcgggcagat gaattcgcaa agctggcagg cgacgcagcg cacgcggccg ccttcatcct
     2941 tcaccagggc gggcgcgccg cgataatgat ccggcatctg gctgtcccat ttctgctcgg
     3001 gatactgcat cgtcaccctg gtgcggccga ggagcatgtt cttcagatgg cggaaagtga
     3061 tgatcatccc gctgaaaatc gccggcagat agaccttctc ggaccagctc agattgggac
     3121 gtcgaacagt gatgggcatg ggctaaatga cgaaatccga atgaagaatg acgaaggaat
     3181 gacgaaggcc caatgacgaa acgaggccgg cgctagacgc ctttcgggct tcgtcattga
     3241 aaagcccggc ctcgccggtg gctgcgttat aggtcattcc ttcggatttc gtcattcatt
     3301 tcggcagaca ggcgatgatg atcgcggcga ggaaaatgtt cacgagcgcg atctcgaaaa
     3361 agaaaaccca gcccagccgc atcagttgat cgtagcggaa acgcggcagc gtccagcgga
     3421 cccagataaa aaagaggagc aggacggaaa ctttgccgaa gaagacaaag atgttgaaga
     3481 ggccgccgat gacgccgcgc catccgccta acgagccatc gatcaggagc cagtgaaagc
     3541 cgtgcgcttg attccaccac gggacgggca gatgccagcc gccgagaaag agggtcacaa
     3601 taacggccga gccggtgatc atggcggcat attcgccgag gaaaaagaga gcaaatttca
     3661 tcgagccgta ttcggtgtga taaccgccga ccagttccgt ctccgcttcg ggcagatcga
     3721 acgggagacg attcgtttcc gcgaaaatcg cgatcgtgaa gacgacgaag gaaataaaca
     3781 tcgggatggc gaggagccac ttccagccgt cggcccaatg gcccacgaag ggtgcgatca
     3841 tccagccatt ctcaatctgg tattggacga tattggtgag gcgcagctgc ccgacgagga
     3901 ggaagatggg aatgaccgcg agcccgaggg aaagctcgta ggagatcatc tgcgagctgg
     3961 agcggacgcc gccgaggaag ggatatttcg aattcgacga ccagccggcg atgacaatcc
     4021 cgtagacgcc taacgacgca atggcgaaaa cgtagagaac gccgacgttg atgtccgcga
     4081 tcaccattgg cacgccgaac aaggtgctgc cgaaaggcac gaccgcgatc gtcatgatcg
     4141 cgggcaccat tgccaggcag ggggcgagcc agtagtagaa cttgttcacg ctggccggcg
     4201 taaagtcttc cttgaggatg aatttcatgg cgtcagcgat cggctggccg aggccgccaa
     4261 ggaacggcga aaagtccttc ttgaatccga gcaggctcac gggaaagccg acgcgattcg
     4321 ggcccacgcg atcctgaatg agcgcgctca cgcgccgttc tgcataaacg gtgtaggaga
     4381 ccaacggaag gatcacgacg aagacgatcc cgaggatctt gagcagcgaa gtaaggatga
     4441 gaaagagcgt ggggttcata ggtcatcccg agcggagtcg agggatcccg cggaggaact
     4501 taagggttgc gccacgggat tcttcgactg cgctgcgctt cgctctgaat gacgaatcat
     4561 tgcggctgct tctccagttc gcgttcgcgc atttctcccc gcgccgggtc gaccggcggt
     4621 ccgtcggcgt ggagcactgg cacgccgaga tcgccgatcc ggctcagggt gagaccggaa
     4681 aactcgggca ctgcctcgct catctggcgg aaaacatctt caatcgtgga gagtccgcgt
     4741 gcgcgcgcct gaccttcgga gtctccgccg gagatcgcct gcaccaggtc gcgcaaaatc
     4801 tcccaatcgt cgcgcgcctc ggcggggccg cggacggcgc gattgagccg ttgcaggcga
     4861 cctctgcggt tgatcatcga gccgcgcttt tcggcgaagc ctaacgacgg cagcacgaca
     4921 ctggcatggc ccgtcgcgtc gttctcgagc agatccagca cgacataaac cggcaggcgg
     4981 gccaggtact cgggcgtgat tccgcaatgc agcggatttt cgcccaacgt gatgagggcc
     5041 tgaatctggc ctgaccaaac cgcttccttg atctgttgca ggacggttcc cgaccgagac
     5101 gcgagccgca ggagacgggc gccgttggtg ttcgggttgc ggtctgcgct cagcaaaatg
     5161 tcgtcggact cgccggcccg cgggacgatg tcgatgagcg tcgcgcctaa cgactcggcg
     5221 agacgcccgg cgagccacaa ctcttcgttc gtcatgcggc cggaggcaag gatcgcggtt
     5281 tgccagccga catagggttt caactgcgcg gccgcgctgg cgatcgcgtg tcgccaggtc
     5341 gccgctgtca gcttaccctc cgcgcgcacc atcggcgccc gcaaccggcg gtcgttttcg
     5401 aggtaggcaa aattgagccg gccgtagtcg cacatccaat cggaattaac ggcgtcgttc
     5461 tcgcgcggtg tctggcgata gatcacgtct tcccgcgtgc cgatgatcgt gttgcaaccg
     5521 gtcgcgcagc tcgtgcagaa acttttcgtc tctttcaaaa accagacccg catcttgaaa
     5581 cggaaatccg atgaagtgag cgcgccgacc gggcagatat cgaccgtgtt aagcgagtaa
     5641 ttattctcca gccgcttgcc cggatgggcg gtgagcgtgg tgtggctgcc gcgatcgaca
     5701 aacccgagga cgtcgtcgtg cgcgatctcc ttgcaaaaac gaatgcagcg cgagcaaagg
     5761 atgcagcgtt cgtcgtcgag cgtgacgcgc ggaccgagga cgacgttctt cggtttcttc
     5821 actttgttct ccagaaagcg cgattcctgc cggccgtact cgacgctgaa ttcctgaagc
     5881 tggcattcgc ccgcctggtc gcagatcggg caatcgagcg gatgattgat gagcaggaat
     5941 tccatcacgc cgcggcgaca ttcctcgacg agcggcgaat tggtccgcac acccagacct
     6001 tcggaaacgt cggtcgcaca ggaaatctgc ggacgcggca tccagttgat ctccgggtgg
     6061 ctgtcggcgc cgagaatggg tttacggtcg ggccccattt tcggcatgcc catttcaatc
     6121 aggcacatgc ggcagttgcc gggcgaggag agcttcttgt ggtagcaata acgcggcacg
     6181 tagctcccgg cctgctcaca cgcttcgatc aggcgcgtgc ctttgggaaa ttgatgccag
     6241 acgccgtcga tctggacgtt aaccatttgg tgagctgcaa ctgttgctgc gtcgggcatg
     6301 gacgaatcag gaaagcagga aagcaggaga attagaggat gagctccgtg ttggacgacg
     6361 aggcaggacg ctcgcggcag aaacgcttga tcgtgagcgg ggtggtggca aaattaagca
     6421 ggaggccatc gtgaagaccg gtcgctttca gatagctgcg gccgatggcg aaatgaatat
     6481 cttccagcgc gcggatagcc tttaactcca ggacgattag atcctcgacc acaaaatcga
     6541 ggcgatgttc gccgatctgg tggtctttgt agaacaaact gacaggcttc tgcctaacaa
     6601 actcgatgcc ctccaaagcg aactcaacgg cgagcgcttc ttcgtaaatg gattcgagaa
     6661 aaccgggacc caaatcccgg tggacggcga tagcggcgcc gatgacgcgc tgcgtgatgc
     6721 cgcggctccg caaatctttt cctgctttcc tgttttcctg attcat
//
LOCUS       DATMNS010000079         3369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3634820, whole genome shotgun sequence.
ACCESSION   DATMNS010000079 DATMNS010000000
VERSION     DATMNS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3634820"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..370
                     /locus_tag="VNW28_02925"
     CDS             152..370
                     /locus_tag="VNW28_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08907.1"
                     /translation="MKTLILFVGWCILFVLAWPVALLALILFPLVWLLALPFRLLGLT
                     FEALLALVGAILMLPARTFGWGRSTSRG"
     gene            441..704
                     /locus_tag="VNW28_02930"
     CDS             441..704
                     /locus_tag="VNW28_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08908.1"
                     /translation="MSYLLIHHKVRDFDTWKKAYDGHRDARDQAGLKELHLLRNHADR
                     NDIVILFTTEDLERARAFARSDNLRSAMQRAGVVGAPVLLELE"
     gene            830..2527
                     /locus_tag="VNW28_02935"
     CDS             830..2527
                     /locus_tag="VNW28_02935"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacylase"
                     /protein_id="HXA08909.1"
                     /translation="MDCSFRCAVLLGQLMFALDAQAASPLPAQEFDVLIKNGTVYDGS
                     SGDGRKADVALRGDRIAGVGDFKQAKARLTIDAQGLAVAPGFINMLSWSTESLIADGH
                     SQSEIRQGVTTEIMGEGESMGPLNERVREKMLREQGDIHYEIKWKTLAEYLRYLEKRG
                     ISCNVASFIGATTIRENVIGFDDKAPTAPQLAEMRALVRQEMEAGALGIGTSLIYPPA
                     FYAKTPELIELCKVAAQYQGKYISHMRSEGNQLLEALDELIRISREAKIPAEVYHIKA
                     AGKSNWPKVDRLLAKIEEAQKEGLKITANMYTYTAAGTGLDACLPPWTEEGGYPALFK
                     RLRDPATREKIAREVATPSDAWENLYLAAGAPDKILLVGFKTEKLKPLTGKSLAAVAK
                     MRGKNPIETIMDLIAEDESRIDAIYFLMSEENVRKEMAKPWISFGSDEASEAPEGVFL
                     KSNPHPRAYGNFARVLGKYVREEKVIPLGEAIRRLSVLPATNLGLDHRGFLKEGMFAD
                     VVVFDPATIADRATFEQPQQYAVGMKHVFVNGVQVLKDGEHTGATPGRALWGPGKIR"
     gene            complement(2625..>3369)
                     /locus_tag="VNW28_02940"
     CDS             complement(2625..>3369)
                     /locus_tag="VNW28_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08910.1"
                     /translation="SRSFVQTGENVMIGGFIVDGIVAKKVAVRAIGPSLAAAGVSGAM
                     SDPVLQIVDSTGAVIATNDGWNIPGQEVSAYGLAPTDPREPALVASLLPGAYSAIVSG
                     QGNSSGIALFELYDLDAATGRIANISTRSRVEAGDNVMIGGFILSGTTATNVIVRAIG
                     PSLVSNGVKDALLDPTLELYDSNGALLASNDNWRSDQEAAIVATTVPPTDDREAAIVG
                     TLAPGAYSAVVRGANGATGVALVEVYALN"
BASE COUNT          699 a         1014 c         1023 g          633 t
ORIGIN      
        1 ccacgccact gagggcgagg cttccggcga tcaccaaacc aagcaatgtt ctcatgcggg
       61 gattgaacgg cccgccggat cacgagcaca agctttttct tcccgccggt cccgaagttg
      121 acgtcgcgcg gcagcatgaa aaagtatggc tgtgaaaacc ctgatccttt ttgtcggctg
      181 gtgcatcctg ttcgtgcttg cctggccggt cgcgcttctc gccctgattc tctttcccct
      241 ggtctggctt ttggcgctgc cgtttcggct tctcggctta acctttgaag ctctgctggc
      301 gctggtcggc gcgatcctca tgctaccggc gcgcaccttc ggttggggac ggtccacgag
      361 ccggggttga tagccggcag tcgctctgct cgcgcgtgcc catccctgat cggtagacat
      421 tagagacaaa ccaaaacgct atgtcatatc tgctcattca tcataaagtt cgggacttcg
      481 acacttggaa aaaggcctac gacggtcatc gcgacgcgcg cgaccaggct ggtcttaaag
      541 agctgcatct cttgcggaac cacgcggatc gcaatgatat cgtcatcctg tttaccaccg
      601 aagatctgga gcgcgcccgc gccttcgcgc gttcggacaa tctccgcagc gcgatgcaac
      661 gggctggagt ggtcggcgcg ccggtgctac tcgaactcga gtaattctca gggacgcgga
      721 ttacagggaa gcccgagatt cgcggacgag cgatacggcg cgcgctcagg ccggcccgcg
      781 gagtcatttt cgcatcgaca cgacggccgt cgacttcttg aatcgcgcga tggattgttc
      841 ctttcgttgc gccgtcctcc ttggccagtt gatgtttgct cttgacgcac aggcagcatc
      901 tcctctgccg gcgcaagagt tcgacgtcct gatcaagaac ggcacggtct acgacggttc
      961 cagcggggac gggcgaaaag cggatgtggc gctgcgcggc gatcgcatcg cgggcgtggg
     1021 cgatttcaag caggcgaagg ctcgcctaac gattgacgca caagggctcg cggtcgcacc
     1081 cggattcatc aacatgcttt catggtcgac cgagtcgctc atcgccgatg ggcattcgca
     1141 gagcgagatc cggcaaggag tgacgaccga gatcatgggc gaaggcgaat cgatgggtcc
     1201 gctgaacgag cgggtgcggg aaaaaatgct gcgcgaacag ggggacatcc attacgagat
     1261 caagtggaag acgctggccg agtatctgcg ttatctggag aagcgcggca tctcctgtaa
     1321 cgtcgcctcg ttcatcggcg cgacgacgat tcgcgagaac gtgatcggtt tcgacgacaa
     1381 ggcgcccacc gcgccacagc tcgcagaaat gcgcgcattg gtccgccagg aaatggaagc
     1441 gggcgcgctc gggatcggca cttcgctaat ttatccgccg gccttttacg cgaaaacgcc
     1501 ggagctgatc gagctctgca aagtcgcggc gcaataccag ggaaaataca tttcgcacat
     1561 gcgcagcgag ggaaaccagc tcctcgaagc gctcgacgaa ctgatccgaa tcagccgcga
     1621 agcgaaaatc ccggcggaag tctatcacat taaggcggcc ggaaaatcca actggccaaa
     1681 ggtcgaccgg ctcctcgcaa agatcgaaga ggcgcagaag gaaggcctca agatcactgc
     1741 caacatgtac acttacaccg cggccggcac gggcctcgat gcctgcctcc cgccctggac
     1801 ggaggagggc ggctatccgg cgctttttaa acggctgcgc gacccggcga cgcgggagaa
     1861 aatcgcgcgt gaagtggcca cgccgagtga tgcctgggaa aatctttacc tcgccgccgg
     1921 ggcgcccgac aaaattttgc tggtcggctt caagacggaa aaattgaagc cgttgaccgg
     1981 caagtcgctc gcggcggtgg caaagatgcg gggcaagaat ccgatcgaaa ccatcatgga
     2041 cctgattgcc gaggacgagt cgcggatcga cgcgatctac ttcctgatgt cggaagagaa
     2101 cgtgcgcaag gaaatggcga aaccgtggat ttccttcgga tcggatgaag cgtcggaggc
     2161 gccggaaggt gttttcctga aatcgaatcc gcatccgcgc gcctacggaa attttgcacg
     2221 agtcctaggc aagtacgtac gggaagaaaa agtgattccg ttaggcgaag caattcgccg
     2281 gttgagcgtc ctgccggcca caaacctggg actggatcac cgcggctttc tcaaggaagg
     2341 aatgtttgct gacgtggtcg tgttcgatcc ggcgacgatc gccgatcgcg ccaccttcga
     2401 gcaaccgcag caatatgcgg tcgggatgaa acacgttttc gtgaacggcg tgcaggtctt
     2461 gaaagatggc gagcataccg gggcgacgcc gggccgcgcg ctctggggtc cgggaaaaat
     2521 tcgttaatca ttttccctgc gacgggtctg gctggcatcg ccgccatgtc gaagtcgact
     2581 cccctcgcct aacgaaatcc caaaccggct ttctcgccgc tttcctagtt gagcgcgtag
     2641 acctcgacca gcgcaacccc ggtggcgcca ttcgcgcctc ggaccaccgc cgagtaagcc
     2701 cccggcgcaa gggtgccgac gatcgcggct tcgcggtcat ccgtcggcgg aaccgtggtg
     2761 gcgacgatgg ccgcctcctg atcactccgc cagttatcat tcgaggccag cagcgcgccg
     2821 ttgctgtcgt agagctccaa cgtaggatcg agcaaagcat cctttacacc gttcgagacc
     2881 agggaaggcc cgatcgcgcg aacgatcaca ttagttgcgg tcgtcccgct cagaatgaaa
     2941 ccgccgatca tcacgttgtc gcccgcttcc acgcggctgc gggttgagat attcgcaatc
     3001 cgacccgtcg cggcatccag atcgtacagc tcaaacaagg cgatgccaga cgaattaccc
     3061 tgaccactga cgatcgcgct gtaggccccc ggcagcagac tcgcaacaag cgccggttcg
     3121 cgcggatcgg tgggcgcaag accgtacgca ctcacctcct gccccggaat attccaaccg
     3181 tcgttcgtgg cgatgacggc tccggtagaa tccacgatct ggagaacggg gtcgctcatc
     3241 gctccgctga cgccggctgc ggcgagggaa ggcccgatgg cgcgcacggc cactttcttg
     3301 gccacgattc cgtcgacgat aaagccgccg atcatgacgt tttcacccgt ctggacaaag
     3361 gagcggctg
//
LOCUS       DATMNS010000080         3327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_822700, whole genome shotgun sequence.
ACCESSION   DATMNS010000080 DATMNS010000000
VERSION     DATMNS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822700"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            271..2670
                     /locus_tag="VNW28_02945"
     CDS             271..2670
                     /locus_tag="VNW28_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXA08911.1"
                     /translation="MKSKSAAEAGRGPSGLAPGRTLTRNGKAKRRGRCGLREKDSPNP
                     PDDRYRTLFDANPTPMWIYDLETLRFLEVNAAAIAQYGYSREEFLARTARDIRPGQQL
                     AQFQQLYLQEGGAGFRGRWRHLRKDGTTIFVSIYTSPLTFGGRPARLVVTTDVTEQQV
                     ADEKIRQSEANLALAQRVARIGSWEVALTNREERANHPPHWSNETFRIFGLDAATTKV
                     THDSFFRLLHPDEREKARTTFLQQLHTGESCKMDHRIIRPDGSERVVHVQSDTLRDAA
                     GRMVKVGGTVQDLTEQRHAEQLLHQQARLINLASDAIFVRNLDGTVRTWNQGAEHLYG
                     WTAEEMCGRQTRNLIYDDPVPYDDATEELLRTGEWRGEMTHLCKDGRKVMVSARWTLV
                     RDERDEPESILIIHTDVTEQKKLEAQFLRSQRLESIGTLAAGVAHDLNNILAPILLVT
                     PLLRGDLEGAEKESFLALVQTSAERGANLVKQMVTFARGADGERVLVQPSHLIEEVVK
                     IAGQTFPKTITLRTKYPRDLWMVEVDPTQLQQVLLNLCVNARDAMPDGGVLRLTSENF
                     TVDEHYANMAAGARPGPHVLIQVTDDGKGIPRHVIDKIFDPFFTTKEVGKGTGLGLST
                     VIGIVKSYGGFVNVYSERGHTCFKVFLPASQVASAAEDCRDAPCVPNGRGEKILIVDD
                     EPCIRAAAEALLERWGYEPLVAEDGVSALALYAQQPKEIALVLTDLVMPLMDGLMLIR
                     ALRKLNPKQKIILSTGREDDLESSEIRSLGVDACLMKPHSREKLLNTLEETLRCEPVI
                     A"
     gene            complement(2698..3195)
                     /locus_tag="VNW28_02950"
     CDS             complement(2698..3195)
                     /locus_tag="VNW28_02950"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXA08912.1"
                     /translation="MNTSPNDITGQHAVWMHEAVSLGRKGMTEDGGGPFGAVVVCNGK
                     IVGRGHNQVTVLLDPTAHAEVMAIREACRSLGRFDLKGCQLYTSCEPCPMCLAAIYWA
                     RLDRVFYACTRHDAAEIGFDDDFIYEQLPLAIAARSLPMEQIERDYSLALFREWMAKP
                     DKVPY"
BASE COUNT          698 a         1010 c         1043 g          576 t
ORIGIN      
        1 tattggggct gccgccggcg gcgttgacct cgatttcgac ctcgtaagcg tccacgccgt
       61 aaacagcagc ggaataaatc ctggagagca cgggtgcgac agtgtcagaa gacgaggacg
      121 aagtccaaac tgcggttgaa aaaaatactg ggcgcgaccc cggtcgcggc caacatcgac
      181 gaaggcttgt ggcacaccgg ttggccgaaa tcctgctttc gacaccctcg acgtaaaaat
      241 cccaggttaa cggcgcccga tcgaatcgag atgaaatcca aatcagcagc ggaggccggt
      301 cgcggaccga gcggcctggc gccaggccga accctcaccc gaaacggaaa ggccaaaagg
      361 cgaggacgct gcgggctccg ggaaaaggac tcgccgaatc ctccggacga caggtatcgc
      421 accctcttcg acgcgaaccc gacgccgatg tggatttacg acctcgagac gttgcgcttt
      481 ctcgaagtca atgccgcggc gattgcgcag tatggctata gccgggagga attcctggcc
      541 cgcacggcgc gcgacatccg gccgggccag cagttggcgc aatttcaaca actgtatctt
      601 caggaaggcg gggcgggatt ccgcggtcgc tggcgacacc tccgcaagga tgggacaacg
      661 atttttgttt cgatctacac cagcccgctg acctttggcg gacgcccggc gcgcttggtg
      721 gtgacgaccg atgtgaccga gcagcaggtc gcggatgaaa aaattcgcca gagcgaagcc
      781 aatctcgcac tcgcccaacg cgtcgcgcgg atcggcagct gggaagtcgc cctaacgaat
      841 cgggaggaac gcgcgaacca tccgccccac tggtcgaacg aaaccttccg catcttcggc
      901 ctcgacgccg cgacaacgaa agtaacccac gacagtttct tccgtctcct ccatcccgat
      961 gagcgtgaga aggcgcgcac cactttcctc cagcagttgc acaccggcga gtcgtgcaag
     1021 atggaccacc gcatcatccg tccggatgga tccgagcggg tggtgcacgt gcaatcggat
     1081 acgttgcgcg atgcggcggg ccggatggta aaagtaggag gcacggtgca ggatctgacg
     1141 gagcagcggc acgcggagca gctactgcat cagcaggcca gactgatcaa ccttgcctcc
     1201 gatgccatct tcgtgcgcaa tctcgacgga accgtccgaa cgtggaatca gggcgcggag
     1261 catctttacg gctggacggc cgaagagatg tgtggccgcc aaacgcggaa tctaatttac
     1321 gacgaccccg tcccctacga cgacgccacg gaagagttat tgcgcacggg cgagtggcgg
     1381 ggcgaaatga cccacctctg caaggacggg cgcaaggtca tggtcagcgc gcgctggacg
     1441 ctggtccgcg acgagcgaga tgagccggag tcgatcctga tcattcatac cgatgtgacc
     1501 gagcagaaaa agctcgaggc gcagttcctc cgctcgcagc ggctcgaaag catcggcacg
     1561 ctggccgccg gagtagcgca cgacctgaac aacatcctcg ccccgatcct actcgtcacc
     1621 ccgctgctgc gcggcgacct ggaaggcgcg gagaaggaaa gtttccttgc tctcgtccag
     1681 acgagcgccg agcgcggtgc caacctggtg aaacaaatgg tgaccttcgc ccgcggcgcg
     1741 gacggtgaac gcgtcctggt gcagccttcg cacttaatcg aagaggtggt gaaaatcgcc
     1801 gggcaaacct ttcccaagac aatcaccctg cggaccaagt atccgcggga tctctggatg
     1861 gtggaagtcg atccgacgca gttgcagcag gtgttgctca atctttgcgt caacgcgcgc
     1921 gatgccatgc ccgacggcgg agtccttcgc ctaacgagtg aaaacttcac ggtcgacgag
     1981 cattacgcca acatggcggc cggcgcgcga cccggcccgc acgtcctgat ccaggtaacg
     2041 gacgacggca agggcattcc ccggcacgtc atcgacaaga ttttcgatcc gtttttcacg
     2101 accaaggaag tggggaaggg gaccggcctc ggactttcga ccgtcatcgg aatcgtcaag
     2161 agttacgggg ggtttgtgaa cgtctacagc gagcgaggcc acacctgttt caaggttttt
     2221 ctccccgcga gccaggtggc gagcgccgcg gaagattgcc gggacgcgcc ttgtgtgccc
     2281 aacgggcgtg gcgaaaaaat tctgattgtc gatgacgaac cctgcatccg ggcggcggcc
     2341 gaagccttgc tcgagcgctg gggctacgaa ccgctcgtcg cggaggatgg cgtctcggcg
     2401 ctcgcgcttt acgcgcaaca gccgaaggaa atcgctctcg tcctgaccga tctggtcatg
     2461 ccgctgatgg acgggctcat gctcatccgg gcgctgcgca agctgaaccc gaaacaaaaa
     2521 atcattctct cgacgggacg cgaagacgat ttggagtcgt ccgaaattcg ctcgttaggc
     2581 gtggacgcct gcctgatgaa accgcattcc cgggaaaagc tgctcaacac cctcgaggaa
     2641 actctgcgtt gcgagccggt gatagcctga gcaggtcggt gccatccgcc ggcggcgcta
     2701 gtacgggacc ttgtccggct tggccatcca ctcgcgaaac agcgcgagcg aataatcgcg
     2761 ctcgatttgt tccatcggga gcgagcgagc tgcgatcgcg agcgggagtt gctcgtagat
     2821 gaagtcgtcg tcgaaaccga tctcggccgc gtcgtgccgg gtgcaggcgt aaaagacgcg
     2881 gtcgagccgc gcccagtaaa tcgcggccag gcacatcggg cagggctcgc agctggtgta
     2941 aagctggcag cctttcaaat cgaatcggcc taacgagcgg caggcttcgc ggatggccat
     3001 gacctcggcg tgcgccgtcg ggtcgagcag cacggtgacc tggttgtggc cgcggccgac
     3061 aatcttccca ttgcagacca cgaccgcgcc gaaagggccg ccgccatcct ccgtcatgcc
     3121 tttccggcct aacgagaccg cttcgtgcat ccaaaccgcg tgctggccgg tgatgtcgtt
     3181 aggcgaggtg ttcatcgggc ctcacttttg catatttccg ggcgcgtttg taaagcctgg
     3241 acatgcgttc aggatcgacg tttatgccgt ggctgaaatc gatctcggga atgggtgttc
     3301 tggctacaga ctggcgagcg cccgatc
//
LOCUS       DATMNS010000081         5684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3635635, whole genome shotgun sequence.
ACCESSION   DATMNS010000081 DATMNS010000000
VERSION     DATMNS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5684
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3635635"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            461..1516
                     /locus_tag="VNW28_02955"
     CDS             461..1516
                     /locus_tag="VNW28_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXA08913.1"
                     /translation="MKIDLHTHILPRAWPDLDARYGYSGFVRMDHYRPCCARMMIGDR
                     FFREVGDNVWDPQRRIEECDRTGVAVQVLSTVPVMFSYWAKPADALDLSRLLNDHLAE
                     VVRAHPARFAGLGTIPLQDPEMAAGELRRCVRELGLRGVEIGSHVDANASCNQEECRN
                     LDDRTLDVVWSAAEEVGAAIFVHPWDMIGKTRMPKYWLPWLVGMPAETSLAICSMIFG
                     GVFERFPYLRVAFAHGGGAFPVTIGRIEHGFNVRPDLVAIDNQINPRHYLARVDEHGE
                     SVPARFYVDALVHDPDALRLLRKLFGVRRIALGSDYPFPLGEAHPGELIESMPDFLPE
                     EKAQLLSGTAREFLALN"
     gene            1529..2212
                     /gene="phoU"
                     /locus_tag="VNW28_02960"
     CDS             1529..2212
                     /gene="phoU"
                     /locus_tag="VNW28_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXA08914.1"
                     /translation="MELPKHILTTFDEALAVLRNNVLLMSSLTARSLENALQGLRERD
                     DELCLQAIADDEEIDQLEKQIDKDGIDLLLRFQPVASDLRRVVAAMKLSPNLERIADE
                     SVSIARKARKLNKHRALPEVELITPMGALATQMFNDGIDAYVREDEAQGRALKERDKP
                     LDLMNSDAARRLLTRAAQDGEQLRGYLNLMFIAGHLERVGDHATNIGEDVVYAAAAED
                     IRHQPFVPE"
     gene            2300..2938
                     /locus_tag="VNW28_02965"
     CDS             2300..2938
                     /locus_tag="VNW28_02965"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HXA08915.1"
                     /translation="MFSIKKLLGHDQKFFDLLEASAQQADSSVHHLVALLARLEKHDS
                     PQSLDEFVHSRRRDKQITQELTEQLCKTFVTPLEREDIQALAAALYKIPKTVERIGER
                     ILICPGDLEGRNFQKHLELLDQAAEAVLAMVKELRKGTDAATAREMNSRLQTIEGDAD
                     KLELDLLRDLYHGNYEAKQIIFLRDLYELLEKVIDRCRDAGNIILQVVLKYS"
     gene            2962..3996
                     /locus_tag="VNW28_02970"
     CDS             2962..3996
                     /locus_tag="VNW28_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXA08916.1"
                     /translation="MVLFIVVVLTALLFEYINGFHDAANAIATVVSTKVLTPRQAIAM
                     AAFFNLTGGLMGTAVASTIGKGMVDLKVVTMPAILCALLGAIVWNLITWLLGLPSSSS
                     HALIGGLCGAALATAGGNFSVLKWRTGLLPKVVVPMISSPIAGFIFGALLMFLLIVLL
                     RPYTPHVVHKLFGKLQLLSAAWMAHSHGTNDAQKTMGIIALALFTGTASGAFQHLPPM
                     FHFLATPEFKVPSWVVVLCAATMAAGTAAGGWRIIRTLGHKMVKLQPVHGFAAETTAA
                     VIIQVASSYGIPLSTTHVISTSIMGVGAVKRFSGVKWTIVERIVWAWILTLPITGLIG
                     YILARAAAAL"
     gene            4176..5291
                     /locus_tag="VNW28_02975"
     CDS             4176..5291
                     /locus_tag="VNW28_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HXA08917.1"
                     /translation="MSAVRLLVGTHKGAFVLTADGRREDWKVSGPHFAGWEIYHLKGS
                     PVNPNRLYASQTSGWFGQQVQRSEDGGETWEPVGKDFVYDGTPGTHQWYDGTQHPWEF
                     KRVWHFEPSLTEADTVYAGVEDAALFRSADGGKTWHELSGLRDAKGQLWQPGAGGMCL
                     HTIVLDPTNPERIFVAISAAGAFRTEDGGKTWQPINRGLKSPFELPDPTAEVGHCVHR
                     IAMHPSRPGVLFMQKHWDVMRSDNAGDDWTEVSGNLPSDFGFPIDVHAHEPETIYVVP
                     IKSDSEHFPPDGKLRVYRSRTGGNEWEALTQGLPQRDCYVNVLRDAMAVDSLDQCGVY
                     FGTTGGQVYASNDAGDTWNAVVRNLPAVLSVEVQTLS"
     gene            complement(5424..>5684)
                     /locus_tag="VNW28_02980"
     CDS             complement(5424..>5684)
                     /locus_tag="VNW28_02980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXA08918.1"
                     /translation="NVAVFQTSGGDVSYGTYLAVNGGTFQFSNLKLTGALINTAGNVQ
                     FVSGSQIGAFVPFEVPEPATFSLLGIGLVAFGIFRKRRLRAA"
BASE COUNT         1091 a         1795 c         1701 g         1097 t
ORIGIN      
        1 gcttggctgc tcttcaaaaa tgcgacctcg gttttccaaa aggcagggtg tgttcctgcg
       61 ttcactgtcc gcgcttttca ggagcaatat cagtcattca aaaagcgtgg tcgctcttcg
      121 gctccgcaga ttgatttgtc ccgctcgcgg cttggattgg ccgaaggcgt gtttgcgggc
      181 gggagaagcg tggttggtcg cggagtccgc aaatcgcgcg gccggaaagc gaggtcgctt
      241 ttccacgaac cgtctctggt tgcgaaaagt ctctggtcca ttcttcacat cgctctttgt
      301 ttttccggac agatgcttct accggtaggc gcctcgatga tccgtcgccg cgtcgaggcg
      361 gcgtcggcag gagcgtacga gaaagccgct tgcccgcgga gacgccgggc gctacaggat
      421 cattctcgtc actggtcacc tgtcactcgt cacttttctt gtgaaaatcg atcttcacac
      481 gcacatcctg ccgcgcgcct ggcccgacct cgacgcccgg tacggctaca gcggattcgt
      541 taggatggat cattatcgcc cctgctgcgc gcggatgatg atcggcgacc gcttttttcg
      601 cgaggtcggc gacaacgtct gggacccgca acgccggatc gaggagtgcg atcgcaccgg
      661 cgtcgccgtc caggtgctct caaccgtccc ggtgatgttc agctattggg cgaaaccggc
      721 cgacgcgctc gatctttccc ggttgctgaa cgatcacctc gcggaagtgg tgcgcgcgca
      781 tccggcgcgc ttcgccggcc tgggcacgat tccgctgcag gatccggaaa tggcggcggg
      841 cgaactgcga cgctgcgtcc gcgagcttgg gctgcgtggc gtggagatcg gctcgcatgt
      901 cgacgccaac gcctcttgta atcaggaaga atgccgcaac ctcgacgatc gcacgctcga
      961 cgtcgtctgg agcgcggcgg aggaagtcgg cgccgcgatc ttcgtgcatc cgtgggacat
     1021 gatcggaaaa acgcgaatgc caaaatactg gctgccctgg ctcgtcggca tgccggccga
     1081 gacttcgctc gcgatttgct cgatgatttt cggcggcgtt ttcgagcgct tcccttatct
     1141 gcgcgtcgcc ttcgcccacg gcggcggcgc cttcccggtc acgatcggcc gcatcgagca
     1201 cggttttaat gtccggcccg acctggtcgc gatcgacaac cagatcaatc cccgccatta
     1261 cctcgcgcgc gtcgatgagc atggcgagtc tgtgcccgcc cgcttctacg tcgacgcgct
     1321 ggtccacgat cccgacgcgc tccgcttgtt gcgcaaactt ttcggcgtgc ggcgcatcgc
     1381 cctcggctcc gattatccct ttccgttagg cgaagcgcac cctggcgaat tgatcgaatc
     1441 gatgccggat tttttgccgg aggaaaaggc gcagcttttg tcgggcacgg cacgggagtt
     1501 tctggctctg aactgacgcg ctcgcgagat ggaactgcca aagcacatcc tcacgacctt
     1561 cgatgaagcg ctcgccgtgc tgcgcaacaa tgtccttctc atgtcgagcc tgaccgcgcg
     1621 tagtctcgaa aacgcgctcc agggattgcg cgaacgcgac gacgaactct gtctgcaagc
     1681 gatcgcggac gacgaggaga tcgatcagtt ggaaaagcag atcgacaagg acggcatcga
     1741 cctgctcctc cggtttcaac cggtcgcctc ggatctgcgc cgggtcgtcg cggcgatgaa
     1801 actgtcgccg aatctcgagc ggatcgccga cgaatccgtc agcattgcac gcaaagcgcg
     1861 caagctaaac aagcatcgcg cacttcccga ggtggaactg atcactccga tgggcgcgct
     1921 cgcgacgcag atgttcaacg atgggattga cgcttacgtg cgcgaggatg aagcgcaggg
     1981 ccgggctttg aaggagcgcg acaaaccgct cgatttgatg aacagcgacg ccgcccgacg
     2041 cctcctcacg cgcgccgcgc aagacggtga gcagttgcgc ggctatctca acctgatgtt
     2101 catcgccgga cacctcgaac gcgtcggtga ccacgccacc aacatcgggg aggatgtcgt
     2161 ttacgcggcg gccgcggagg acattcggca tcagcctttt gtcccggagt aaatctccga
     2221 tcgagcgcgc gtcgcttcag tttcggcttg gcaccggcgg gcggttgcgt tacggcagac
     2281 gcacaacccg agcggccgca tgttctcaat caaaaaacta ctgggccacg atcagaaatt
     2341 cttcgacctt ctcgaagcca gcgcgcagca ggccgacagc agcgtgcatc atctcgtcgc
     2401 gctcctcgcc cggctcgaaa agcatgattc gccccaaagc ctcgacgagt tcgtccacag
     2461 ccggcggagg gacaagcaaa tcacgcagga actcaccgag caactctgca agactttcgt
     2521 cacgccgctg gaacgggagg acattcaggc gctcgcggcc gctctctata agataccgaa
     2581 gacggtggag aggatcggcg aacgcatcct gatctgtccg ggcgatctcg agggccgcaa
     2641 tttccaaaag catctcgagt tgcttgacca ggcggccgag gccgtcctcg cgatggtgaa
     2701 ggaattgcgc aaaggaaccg acgccgcgac cgcgcgggaa atgaattcgc ggctgcaaac
     2761 gatcgaaggc gacgccgaca aactggagct cgatctcctg cgcgacttgt atcacgggaa
     2821 ttacgaagcg aagcagatca tctttctccg cgatctttac gagctgctgg aaaaggtgat
     2881 cgatcgctgc cgcgacgcgg gcaacatcat cctgcaggtc gtgctcaagt actcctgagc
     2941 tcctgctcac agggcccgcg catggttctc ttcatcgtcg tcgttctgac ggccctcctt
     3001 ttcgaataca tcaacggctt tcacgacgcc gcgaacgcca tcgcgaccgt cgtgtcgacc
     3061 aaagtcctga ccccgcgcca ggcgatcgcg atggcggcct tcttcaacct gaccggcggc
     3121 ctgatgggga cggcggtcgc ctccaccatc ggcaagggca tggtcgacct gaaggtcgtg
     3181 accatgccag ccatcctttg cgcgctgctc ggcgcgattg tgtggaatct tatcacctgg
     3241 ctgctcggcc ttccttcgag ctcgagtcac gcgttgatcg gcggtctctg cggagcggcg
     3301 ctggcgaccg ccggcggtaa tttctccgtc ttaaaatggc ggaccggtct ccttcccaag
     3361 gtcgttgtcc cgatgatcag ctcgccgatc gcaggattca tcttcggcgc tttgctcatg
     3421 ttcctcctca tcgtcctgct gcgcccttac accccccatg tcgtgcataa gctctttggc
     3481 aaacttcaac tgctgagcgc ggcctggatg gcccatagcc acgggactaa cgacgcgcaa
     3541 aaaacgatgg gcatcatcgc cctcgccctt tttaccggca cggccagcgg cgcgttccag
     3601 cacttgccgc cgatgttcca ttttcttgcg acgccggaat tcaaggtccc gtcgtgggtt
     3661 gtcgtcttat gtgccgcgac gatggccgcc ggcaccgccg ccggtggctg gcgcattatc
     3721 cgcacccttg gacacaagat ggtcaagctc cagccggtcc atggcttcgc cgcggaaacc
     3781 accgccgccg tcatcatcca ggtcgccagc tcttacggca ttccgctttc cacaacccac
     3841 gtgatctcga cctcgatcat gggcgtgggc gcagtcaaac gcttcagcgg agtcaaatgg
     3901 acgatcgtcg agcggatcgt ctgggcctgg atcctaaccc ttccgatcac gggactgatc
     3961 ggctacattc tggcgcgggc cgcagccgcc ttgtaaagat cgacgacttg tcgagccatc
     4021 gctttatcgc gagcgcgggc cataagaaaa aggacccgtt ccccggacaa aaccgaagca
     4081 gtcgccgtcg ttagggaaga ggagcgcgaa ttcccgcagc cagccgggtt gagcaaatta
     4141 gttgctccgg atcgcagcgg cgctaaaaat ggaggatgag cgcagtccga ttactggttg
     4201 gcacacacaa gggcgccttt gttctcaccg cagatggcag gcgcgaagat tggaaagtga
     4261 gcggcccgca cttcgcgggt tgggaaatct atcatctcaa gggctcgccg gtgaatccca
     4321 accggctcta cgcatcgcaa acgagcggct ggttcgggca acaggtccag cgctccgagg
     4381 acggcggcga aacgtgggaa ccggtcggca aggacttcgt ctatgacggc actccgggca
     4441 cccatcaatg gtacgacggc acgcagcatc cctgggagtt caagcgggtc tggcatttcg
     4501 aaccatcgct caccgaagcg gacacggttt acgcgggagt agaggatgca gctctctttc
     4561 gcagcgccga tggcggcaag acctggcacg aattgtccgg tttgcgcgac gccaaagggc
     4621 aactctggca accgggcgcg ggcgggatgt gtttgcacac catcgtcctc gacccgacga
     4681 atcccgagcg catcttcgtc gccatctccg ccgcgggcgc ctttcgcacc gaggacggcg
     4741 gcaagacctg gcagccgatc aatcgcggat tgaaatctcc cttcgaacta cccgacccga
     4801 cggcggaagt cggccactgc gttcatcgca tcgcgatgca tccgtcgcgc cccggcgtgc
     4861 tcttcatgca aaagcattgg gacgtgatgc ggagcgacaa tgccggcgac gattggacgg
     4921 aagtgagcgg gaacctgccg tcggatttcg gatttccgat cgacgtccac gcgcatgaac
     4981 ccgagacgat ttacgtggtc ccgatcaaga gtgactccga gcatttcccg ccggacggga
     5041 agctgcgcgt ttatcggagc cggacgggcg gcaacgaatg ggaagccctc acgcagggtc
     5101 tgccgcagcg cgattgctac gtgaacgtgc tgcgcgatgc gatggcggtc gattcgctcg
     5161 accaatgcgg cgtctacttc gggaccacgg gcgggcaggt ttacgcttcc aacgatgccg
     5221 gtgacacttg gaacgcagtg gtccgaaacc tgcccgctgt tttatcagtc gaagttcaga
     5281 ctctctcgta accgcacccg gcgtccaagc ttgacccgag ccaaatcgcc gccgccccag
     5341 ccccgcgatc tggcttcacc gaccgcgcct tcggggaagc gccgttggcc cgagggtcct
     5401 gcctgttccc cgacgatcgc ttcttaggcc gcccgaagac ggcgtttgcg aaagatgcca
     5461 aaagcgacca atccgatccc aaggagacta aaggtggcag gttccgggac ctcgaaagga
     5521 acgaaggcgc cgatttgcga ccccgaaacg aactggacat ttccggcggt gttgatcagc
     5581 gcgccggtaa gcttgaggtt cgagaactgg aaggtacccc cgttcaccgc caaatacgtc
     5641 ccgtacgata catcaccccc ggaggtctga aacacggcca cgtt
//
LOCUS       DATMNS010000082         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_823730, whole genome shotgun sequence.
ACCESSION   DATMNS010000082 DATMNS010000000
VERSION     DATMNS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823730"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            917..2071
                     /locus_tag="VNW28_02985"
     CDS             917..2071
                     /locus_tag="VNW28_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXA08919.1"
                     /translation="MQLTRTAFGMWSAGRFMHFGEPLEDDRFIALIRLAYEKGIRTFL
                     TADVYGAGGADTLLGRALDGISRDSYCLVGAVGHDFYSGQRDGAKGFPRFTSPALRKP
                     GEYASYLRAATEKSLARCRSDRFDLLLLHNPDATGYTSDAVWKGMEKLKDAGLTTRLG
                     VAPGPANGFTLDLILCLERFQGLIDWAMIILNPLEPWPGSLGLPAAEKTGTKIVTRVV
                     DYGGLFHDDVKPGHQFAPRDHRTFRPAGWVEAGAEKIETMRAIADAHGLTMLQLACIW
                     NLAHGPVQSVVPTLIQEAGAQAKPIETKVAELASLPEMVLTPDEFARIAEIGDNTNCM
                     SLKGASAEHTGDALPDRWSLNAEFEAVAGRWKIDPVHDLAYAINPAGAVD"
BASE COUNT          452 a          634 c          633 g          412 t
ORIGIN      
        1 cacgcaaaag gacgagatcg aagcgaccgg cattgcgccg agcgacccaa gcgaatccgc
       61 gatcctcgcc acggtcacgg ggggcggcta cacagcgatc gtggccgggg caaacggcac
      121 cgtcggggtg gcgctggtgg aagtttacaa gctttcctcc tcgccgtagg aggcacttcg
      181 acgccgcgct gtcaagctgg ggcgtgggtt cggccgaatc cgccagcgat cgaaggtggc
      241 gtgacagatc acgtttggga gatcggcgaa attgtcgcgt tgctcgattc aggcaacgca
      301 ccaaagcggc aaacaggaag ggagctataa aaatgccacg aaatgttgcc gaataagcgt
      361 atgcagcggg cttttccggc ccgggcaact ctgccaagat gaggggatga aaggcttggc
      421 gtgcttcttc cttttttgtg tcttggcagg gggtgcgcat ctccgtcacc tcatattctg
      481 cgtggcagcg ggatatttga tatttatttt gatgcgagcg gccaagttgc agatgtgcgc
      541 gtcgtcaaga gcaccggcag cgaggttctt gacgctacct cggttcgcac ctttcggaag
      601 tggcatcaca aaccaggaaa atccgcccga gtaagagtgc ccatcacata taccctggac
      661 aaaccgccgc cactgtcgaa aaaaccataa cgaatcagac ccaacaccca aactagtgcc
      721 ctgtatcact tgctcatatg gcagccgttc gttcttttcg cgccgtgtcc gtcgccgtgc
      781 atatacgtga ctcacgtata tcaaacgcaa agttcattgt gatgcaaatg acggatacgt
      841 gccactatta cactaccagg tttatgaggt gcgagcatcg ggtattatgt ttcaatagct
      901 tggcgatttt atggggatgc aactgaccag gactgctttt ggcatgtgga gtgccggacg
      961 tttcatgcac tttggcgaac cgctggagga cgaccgcttc atcgcgctca tccgcctcgc
     1021 ttacgaaaag ggaatccgca ccttcctgac agccgacgtt tacggcgcgg gcggagccga
     1081 taccctgctc ggacgcgccc tcgacggaat ttcccgcgac agctattgcc tggtcggagc
     1141 ggtcgggcat gatttctatt ctggccagcg cgacggcgcg aaaggctttc cccgtttcac
     1201 gagccctgcg ctgcgcaaac cgggcgaata cgcgagctat ctgcgcgcgg cgaccgagaa
     1261 gtctctggcg cgctgccgga gcgaccgatt cgatcttctt ctcctgcaca atcccgacgc
     1321 caccggctac acgagcgacg ccgtctggaa agggatggaa aaactaaaag acgccggcct
     1381 aacgacacga ctcggcgtcg cgcccggccc ggcgaacgga tttactctcg acttgattct
     1441 ctgcctcgag cgcttccagg gtttgatcga ttgggccatg attatcctca atcccctcga
     1501 gccgtggccc ggcagccttg gcctgccagc cgcggaaaaa accggaacga agatcgtgac
     1561 gcgggtggtg gattatggcg ggcttttcca cgacgatgtg aaacccggcc accagttcgc
     1621 gccgcgcgat catcggacgt ttcgtccggc cggctgggtc gaggccggcg ccgaaaaaat
     1681 cgaaacgatg cgcgcgatcg ccgacgcgca cggtctcacg atgttgcaac tggcctgcat
     1741 ctggaatctc gcacatgggc ccgtgcaaag cgtggttccc accttgatcc aggaggccgg
     1801 cgcgcaagcg aaaccgatcg aaacaaaagt cgcggagctg gcctcccttc ccgagatggt
     1861 cctgaccccg gacgaattcg cgcggatcgc cgagattgga gacaacacga actgcatgag
     1921 cctcaaaggc gcgagcgctg aacacacggg cgatgcgctg ccggaccgct ggtcgctcaa
     1981 cgccgagttc gaagccgtcg ccggtcgctg gaaaatcgat cccgtgcacg acctggccta
     2041 cgccataaat ccggcggggg ccgtcgatta aagctgagtt ggcgcgatca atccgtttcc
     2101 ttccgacgcg ggccggacga tggccccaat a
//
LOCUS       DATMNS010000083        26286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2616537, whole genome shotgun sequence.
ACCESSION   DATMNS010000083 DATMNS010000000
VERSION     DATMNS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 26286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26286
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2616537"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1420)
                     /locus_tag="VNW28_02990"
     CDS             complement(<1..1420)
                     /locus_tag="VNW28_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08920.1"
                     /translation="MSARLLAFCGGAVLSVTAVLAHGPPRMRATPSYHNDVSPPLREM
                     AATDSPERHQDREAAENPKIPNSHVDRPDPVIDKGSILRFLAPSIPAPILGFDGIAFP
                     GVSCSCAPPDPNGAVGLTQYVQMVNEGYQVFDKATGNSVLGPNSIVSLWSGFGGACET
                     GGFGDPVVLYDQLADRWVISQFASATGGIPLTDECVAVSTSGDATGTYSRYGFHLGSN
                     FFDYPHLSVWPDAYYMSMNVFNPAGTAYLGPQPFAFDRTAMLTGAPATFISPGGPLGG
                     SVAPFLPANLDGFTPPPAGAPNTFVGFPGGGSYPTYHFHVDFTTPANATFTSFNTPAA
                     AGFTQLCPATRSCVPELGVTSSDKLDGIGDRLMFRLAYRNFGDHESLVGNFTVNAGGA
                     AGIRWFELRGVTAGPVTVFQESTYQPDTTSRWMGSAAMDGQGNLAIGFSASDGTINPQ
                     LRYAGRLVTDPLSSLAQGEAHLF"
     gene            complement(1575..2906)
                     /locus_tag="VNW28_02995"
     CDS             complement(1575..2906)
                     /locus_tag="VNW28_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXA08921.1"
                     /translation="MQPGSPAHGPRTISLLTASCIVVANMVGTGIFTSLGFQVGGLPT
                     GFAIMALWTVGGICALCGALSYAELGAALPRSGGEYHFLREIYHPSIGFVAGWISATV
                     GFAAPVAIAAIPFGTYISGVVPGTNPLFLSLAVVWITTLVLLWDLKLGSGFQNISTLL
                     KVALILVIIAAGFYVRGSQPISFLPVKTDRALLLSPPFAISLYWVMYAYSGWNASTYI
                     VGEVRNPTRTIPLSLGLGTLLVTALYLGINAVFLRTTPAAEMAGKQEVGLIAGLHIFG
                     TDGGKMMAVFIALGLVSTVSAMMWIGPRVMMTMGEDLSALSWLSRRNGRGLPIRATLV
                     QFAIVNLLILVTTFQKVVNYVQFSLTLFSALTVLGVFVLRWRRPALPRPYRTWGYPVT
                     PAIFLAVSGWMLWHLLEDPSTREPSLWGLATAALGLMIYYLSPKKTPNGVF"
     gene            complement(3044..3463)
                     /locus_tag="VNW28_03000"
     CDS             complement(3044..3463)
                     /locus_tag="VNW28_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HXA08922.1"
                     /translation="MKSKKLILFTLSSAAVLAFGQFAGAQTPDCAPAWHHHGPPDMTA
                     MLSHALNLTDAQKAQVQTMVAAVQPQLEAIHQQAHAQAAPIIKQLQPQLEAIHQQARA
                     QAAPIIKQLHTQIRPLLTADQQKKLDALETLHDSGSE"
     gene            3646..4242
                     /locus_tag="VNW28_03005"
     CDS             3646..4242
                     /locus_tag="VNW28_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXA08923.1"
                     /translation="MPDEKFDEQACLRRVAEGDEHAARELLRHFHPFVLKLARSHLPR
                     RISEEDLTQMIFIRIFQNLDSYSGKVPLEHWISRVAVNTCLNELRAEKRRPEWRMADF
                     DEETSAAIERLAAIETDLASPDDARRAKELLAQMLAQLSPEDRLLITLLHLEERSVEE
                     IHALTGWSRPAIKVRAFRARAKMKKMLQQTANREMIFN"
     gene            4242..4547
                     /locus_tag="VNW28_03010"
     CDS             4242..4547
                     /locus_tag="VNW28_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08924.1"
                     /translation="MDAHQLLIRIFSLARRVPDVPPNEMPFGQETAVLAHWREASARR
                     AAGAALLRGLRWAALTACAVAVLAAMLAKDELVAFQNRFDPETRVADSAIVDGYGHE"
     gene            4540..4968
                     /locus_tag="VNW28_03015"
     CDS             4540..4968
                     /locus_tag="VNW28_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08925.1"
                     /translation="MSKARSGRVILYALLIFLAGAVTGVLVAPMVGRSFIRPPQAKDM
                     SHHMLEYLQSGLNLTAQQTAQIKPLIEKTGADMEVIHHETMKRVLARLAQTHAQISVL
                     LTPEQQAKFLQMQAEHRKQMRHDHPFLSPPGPPPPPPSGP"
     gene            complement(5413..7041)
                     /locus_tag="VNW28_03020"
     CDS             complement(5413..7041)
                     /locus_tag="VNW28_03020"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXA08926.1"
                     /translation="MTAESSTLLIVGAGPVGLTMANELARHGVRCRIIERAPQPSQTS
                     RALAIFPRTLEAFATMGIVDRFLERGHRIHGLSLHHRAEQIAQIELTSVASPFPFALG
                     VPQSETERLLTEQLASRGVEVERNVELTGLTQTSDAVRAKLRHADGREEEMETPWLIG
                     CDGAHSTTRHSLGMHFEGAQYDESFILADVHLDSPLTCDRVHLYLGNDGVLGVIFFEQ
                     NRCRIIANIPPETRGQTLPDLSLADVQELIDQRADASFRAHDPVWLSRFHISHRKVRQ
                     FRQMRVFLAGDAAHIHSPAGGQGMNTGIQDAFNLGWKLALVVRGIAGPQLLASYHVER
                     EPVASGVLNLTDRITRLATMRNSVAQSVRDFLLPMVSGIDFVEEKIADRLAELNVSYR
                     TSPIVENYGAGRLKAGDRAPDADLRDENNQARRLFDLFREPRHVLLIFLGATPTSAAP
                     AEQLRSVLVTMPEGTIDSYRIARARTGAEAELHDLSGLAHGAYGLFEGGVALVRPDGY
                     VGYRAADFNAPRLQSYLARNFGNPSAALLQAEAG"
     gene            complement(7084..8001)
                     /gene="ligD"
                     /locus_tag="VNW28_03025"
     CDS             complement(7084..8001)
                     /gene="ligD"
                     /locus_tag="VNW28_03025"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HXA08927.1"
                     /translation="MSKKAQLVVEDRTLEVSNLDKVLYPKDGFTKGELINYYIQIAPV
                     LLPHLKDRPLTMKRYPDGVEGEFFYEKNCPSHRPKWVQTARVWSESNERNIDFCLAQD
                     LPTLVWAANLADIELHTSLSRKKALARPTMMVFDLDPGAPADIMQCCQVGLWLREILQ
                     GMKLESWAKTSGSKGLQVYVPLNTAVSYEQTKELSHAIAEHLEREHPDLVVSRMSKAL
                     RPGKIFVDWSQNDEHKTTICVYSLRARDKPTASTPVSWKEVEECARTKKAKLLVFRCD
                     ETVARAGKMGDLFASVETLKQKLPKKWPW"
     gene            complement(8089..9279)
                     /gene="lpxK"
                     /locus_tag="VNW28_03030"
     CDS             complement(8089..9279)
                     /gene="lpxK"
                     /locus_tag="VNW28_03030"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HXA08928.1"
                     /translation="MHRWLENLEQFAIDVILGRQHGARAALLRSLLYVLSLLYARIVQ
                     LRLFLYRKRIFRERALGCLVISIGNLTVGGTGKTPIVEKFARALQAGGRRVAILSRGY
                     KSVAKPSKRSLLDRLRGVAPDPPRVVSDGQLLLLDSLTAGDEPYMLASNLKDVIVLVD
                     KDRVKSGLHAIREMKADTLLLDDGLQYLHLKHRLDIVLIDRQAPFGNEFLLPRGTLRE
                     PPRNLRRASYIFITKSTGEPNDELIARIRRYNRTAEIIECAHQPLYLQDVYTGEQLPL
                     GRLTDTYIGSFCGIAAPESFEDGLRKLGARLELAKRFTDHHRYSEPELQSFINRCLRR
                     DVDMIVTTEKDAVRFPRLAAPEVPIYFLRVEILILSGHETWEHCVGRICQPQPMLSPE
                     RFFS"
     gene            complement(9286..10137)
                     /gene="thrB"
                     /locus_tag="VNW28_03035"
     CDS             complement(9286..10137)
                     /gene="thrB"
                     /locus_tag="VNW28_03035"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HXA08929.1"
                     /translation="MKKQQITVRVPATTSNLGPGFDCLGVALQLYNFVTIAPASPPAG
                     DGMVRQAARKFFDAVQRAPFHFSCAITGEVPRARGLGSSVTVRLGVLHGLNAMLDRPL
                     PRAQIFGLCAELEGHPDNAAPAEFGGFTVARHNRHQKFPVDPRLHFVLLIPDFEVETK
                     EARAVLPDEINRADAVESCANACVITAAMASGNYEALRGALRDHFHQPFRAKFVPFLE
                     PVVAAAEKAGALGGFLSGSGSTIACLTLRAPDEVAAAMREASGLREAATRVTTADNEG
                     VRLVVSS"
     gene            10230..11138
                     /locus_tag="VNW28_03040"
     CDS             10230..11138
                     /locus_tag="VNW28_03040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXA08930.1"
                     /translation="MNNWIDFGLVRDFAAEKTEAYRLCSKPDAWVERFGPDALVSYRT
                     EAAREQILHELDEWALLANQKFERVFGRYLPRQNSERNAPQLISGDASVPLQRHVLER
                     NVCYALDFGAGHSAGLFLDQRENRQFVRRAAPRRLLNCFAYTCSFSVMAALAGGETLS
                     VDLSRKSLDRGRDNFILNKLATDRHRFLVGDVLTVLPRLARKGECFDTIILDPPTFSR
                     SHTGKAWQVEHDFEKLLLAALEVAERDAKILLSTNCSRLTERALEVMGRFCLKTTRRA
                     GSFLAPVALPDFPPGAGARSLWMILR"
     gene            11256..11540
                     /locus_tag="VNW28_03045"
     CDS             11256..11540
                     /locus_tag="VNW28_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08931.1"
                     /translation="MDSEQMTGATSDLAERGLRYTREQWEEIVAQTEDYVRANPTRSL
                     LYGVAAGFIIDRLPICRIFGAFVRLLLMAFKPAILVYGAIKLYEAVKAED"
     gene            complement(11578..11787)
                     /locus_tag="VNW28_03050"
     CDS             complement(11578..11787)
                     /locus_tag="VNW28_03050"
                     /inference="COORDINATES: protein motif:HMM:NF043052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin"
                     /protein_id="HXA08932.1"
                     /translation="MSDHVYKKLELVGSSPKSIEAAVQNALSRAGKTIRNMRWFEVVE
                     TRGYVEKNKIDHWQVTIKVGFTLES"
     gene            11906..13855
                     /gene="acs"
                     /locus_tag="VNW28_03055"
     CDS             11906..13855
                     /gene="acs"
                     /locus_tag="VNW28_03055"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXA08933.1"
                     /translation="MEKNIESHLKETRVFKPARAFAKNARVGSLEDYRRMYRESIRQP
                     EKFWAREARELRWQKPWKKVLEWKAPFAKWFVGGKLNVAENCLDRHLQGPRRNKAAII
                     WEGEPGDKRILTYQQLHREVCRFANVLKRNKIRKGDRVIIYLPAIPEAAIAMLGCARI
                     GAVHSVVFGGFSADSIRDRIADCGATAVITADGGFRRGAIVPLKHNVDEALAGGTTIK
                     RVIVFRRAGNEIHIEEGRDVWWHRELEYVDAHCPAVPLESEHPLYILYTSGSTGKPKG
                     ILHTTGGYLVGIYSSTKYVFDLREDDIYWCTADIGWVTGHSYVVYGPLAAGATTVMYE
                     GAPNWPEPDRFWRIIEEYAVSILYTAPTAIRAFIRWGDQWLKKHDLSSLRLLGTVGEP
                     INPEAWMWYHEKIGGGRCPIVDTWWQTETGAIMITPLPGAIPTKPGSATLPFFGVDPV
                     VVDQRGREVGPNVGGKLIVRRPWPAMLRTIYGDRARYQKQYWSEFRGNYLTGDGARRD
                     RDGYFWIVGRIDDVLNVAGHRLGTSEIESALVSHAKVAEAAAVGRPDELKGQGVVVFV
                     TLKSGVLPTPSLKEELRGHVGTHIGAIAKPDEVRFAEALPKTRSGKIMRRLLREIAAG
                     RTVTGDITTLEDFSVLAKLGSRDEE"
     gene            13976..15835
                     /locus_tag="VNW28_03060"
     CDS             13976..15835
                     /locus_tag="VNW28_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08934.1"
                     /translation="MSFISTGFRELTLKVRRQRTRMALKHEKRHLQKSEIALGREGTA
                     EAAKFPEVRDEIVALKKFEQEQKEVAVRIARIEEALKQIESQRQENSRAQNAALAKLE
                     EEKRPLVERRDEAKAAVERCEKELATVDRRLQENDAAERELLKKLSALQATEPAPADF
                     ETQMERIGAERARLPREQAGLVQARLGSVEASRSAREKLTTEEAAVAQADKNIARVRG
                     EFEAHERALQESSRAQQEEVKQARQQHQTVEEKKNPAYLNIGRHLASQGIAPPSAPHL
                     LTEVRHRRGAVDRHAALRQELALLSSQIDKQELRKFYFTIFSLLALLAIILPLISQSP
                     PKRDWLPRETRAIVSLDIDQLNNGPLANRWRKEQPDFWQKVATGLLGPAARTPALNLA
                     RDARRVTRALTIEENGAPGAYVLVETRGDLAPIVRAITQDKSFAQSTMAGLVVWQRPD
                     ISVARVGPKTLAVGSLGEVDKLVEVRLGIQPDLKIDDPLLKSFQALDPDSALRLVAAA
                     PTELSTLLGPILPDAFLKSCALLGLELTLAAPAKARLLVRATEAAKAKELAAGLQNEP
                     GRWLTIPGSDFALSAGPPKVEQKNENLDIRFDLPEGAARLLLQRLAQVPSETP"
     gene            15832..16494
                     /locus_tag="VNW28_03065"
     CDS             15832..16494
                     /locus_tag="VNW28_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HXA08935.1"
                     /translation="MSRFVRLFHWTLGVSSFSFFFFFLLVPPAAIARPTYDNLVDIKS
                     VDPTILVELRYATAQNIAGHALYPPDFPALVRPTTAARLTKAQKFLRARHLGLKIWDA
                     YRPVAAQMELWQRTHNGDYVADPYDGNGSLHTWGVAVDATLVDESGRDLAMPTGYDEF
                     TPAAKLRYHGDDPVIKSHLKSLQAAMRTGGFYGTPTEWWHFVAYDWKKYAPMREAKQL
                     TR"
     gene            16502..17011
                     /locus_tag="VNW28_03070"
     CDS             16502..17011
                     /locus_tag="VNW28_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08936.1"
                     /translation="MLSSFLKELSQPEYVHVLINPLPVYGLATGILGLIIALIQRSRH
                     AQVAALALILLSAAIAWPVVHFGEEGYDRVLSMSDDQGQAWLKVHEHRANELVWFFYA
                     LAAVTATAIFAPAKWPKSAMALTLVTLLFSFAVLALGGYIASAGGKIRHREFRNEPAP
                     PVPADDNDH"
     gene            17016..17687
                     /gene="trmD"
                     /locus_tag="VNW28_03075"
     CDS             17016..17687
                     /gene="trmD"
                     /locus_tag="VNW28_03075"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXA08937.1"
                     /translation="MRFDIITLFPEACRVPLSESMMGRAQEAGALELHIHNLRDWTTD
                     RHHVVDDAPFGGGQGMVMKPEPIFAAVESLRTDASTVVLMTPQGRRFAQAMAQDFSAR
                     EHLIIVCGHYEGVDHRVVEHLVDAEISIGDYVLTNGAIAAVVFVDAVVRLLPGVLGDE
                     QSAADDSFSSRLLEAPQYTRPAEFRSWKVPDVLLSGHHAEIAAWRKGEAERRTRENRP
                     DLFSK"
     gene            complement(17748..19742)
                     /locus_tag="VNW28_03080"
     CDS             complement(17748..19742)
                     /locus_tag="VNW28_03080"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HXA08938.1"
                     /translation="MIALDVNSTTREVFGNIAPWMRVVFFIMIAASFAVLGWQLLTHW
                     RHWRKGQTGGFERDWRVWIRRLLAYGLAQKRVHKKTLGALLHLLLFSGFLVLTIGTTL
                     LSIAYDGPYYFHHGWYYLIYELTMDVFGVVFCLGCLLAMYRRAFRRKPSLGHNRSDWW
                     LLSLLLALGLTGFLVEGLRLHYTQVQPWLAHWSTIGWLLDVTLLRGLSVETARSLHLA
                     SWWLHTILVGLLFATLPVNRFLHIITGPLNIALRPQRPMGTLVPLQMQAVEETGLIGV
                     SELDQFTQQQLLSLDACMECGRCEDACPATATGKPLSPKKVVTDLRGLMSLGEGKLNV
                     HETIQAETLWSCTMCQACVQECPVLIGHVDLISDMRRYLVGEGKLSGPPARALQQVGN
                     QANPYGRPNSDRLAWAEGLDVPTVETNPGFEYLFWVGCAASFDPRAQKVARATAQLLE
                     KGGVNFAVLGNEETCTGDPARRIGDEFLFQERAQANVETLGRRKVRKIVTPCPHCHNT
                     LKNEYPQFGGQYEVQHHSTLLAELIRSGKLTNDTSGDEPITLHDPCYLARVNGEVDAT
                     RAVLDAAKDEQFREMPRHGKKTFCCGAGGGRMWFDEPPEQRVSNLRAQEAIETGAKTL
                     ATGCPFCLNMMSDGMAGTPGGENLRVLDIAEVLLSRQTPG"
     gene            complement(19754..20716)
                     /locus_tag="VNW28_03085"
     CDS             complement(19754..20716)
                     /locus_tag="VNW28_03085"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXA08939.1"
                     /translation="MAATLVLIEHDRQQVKGPSLHAITLARQLGGEYALLVLGHGLNE
                     VAASLVSYGASAVIVADDAALAEPLADRYAAVVAQVAQKHGAKTLLGSSSTFSKDILP
                     RAAALLDAPMVTDVLALEEVNGARAYRRPTSAGSMLATVKLEGDRCVLTARATAFEAP
                     TADAAPCSIERFDFAPASLPNGMQFISREQRVSDRPDLTEARVVVSGGRPLKDPETFE
                     RLVGGLADALGGAVGATRAAVDAGMAPNDYQVGQTGKVVAPELYIAMGISGAIQHLAG
                     IKDSRIIVAINKDPDAPIFQVATYGLVGDLHQLVPQLIEAVRRG"
     gene            complement(20719..21444)
                     /locus_tag="VNW28_03090"
     CDS             complement(20719..21444)
                     /locus_tag="VNW28_03090"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HXA08940.1"
                     /translation="MKIVVPFKSVPDPYAGPGAAAKSVVNPFDEIAIEEALRMRERGE
                     ATEIVGVTIGPAIVDEQIRSALAMGVDRALRIDDSRALDPYAVARILRALVEKEAPQL
                     VLMGKQAVDDDSNQAGQMLAGLLGWPQATFISKLEFLENKTRAQCTRETDGGLEVIVV
                     NLPAIVTTDLRLNEPRYVSLPGLMKARKKPIEVLTCEQLGVTVQPLTITLSTARPPKR
                     AAGTRVESVEELMVKLKQEAKVL"
     gene            complement(21542..22687)
                     /locus_tag="VNW28_03095"
     CDS             complement(21542..22687)
                     /locus_tag="VNW28_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXA08941.1"
                     /translation="MDFSWSDQQRELLDAVDRFAKEQLAYDMIENDRHGVFNHDAWMK
                     CAGFGIHGLLVSEEFGGLGLDPITTVAALERLGYGCKDNGLLFSINAHMWTAIIPLVY
                     NGTEAQKKKFLPGLCDGSIIGGNAMSEPNSGSDAFSLATTAVKKGNKYILNGSKIFVT
                     NGPVADVLLVFAATDKTKGASGLSAFLVEKDSPGLDLTHKIEKMGVRTSPMGEVFFTD
                     CEVPAENLLGKEGAGSWLFTRSMTWERGCILAASVGTMQRLLETCIRYANERQQGGQA
                     IGKFQQVAGKIVDMKMRIENSRHMLYQCAWAAGHRKAIYLEAAMAKLQISESWVKCCE
                     DAIQIHGGYGYMVEYEVERELRDAIGSKLYSGTSEIQRNIIASLIGL"
     gene            22895..23404
                     /locus_tag="VNW28_03100"
     CDS             22895..23404
                     /locus_tag="VNW28_03100"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HXA08942.1"
                     /translation="MITVANSSRINRPPRARRTTLRPALWRCPKCRRAFANRNQSHAC
                     GRHKLADHFRDKPREIRALYDHVVAALQAIGPVRILPEKTRIAFQVRMSFAQVTPRQK
                     WLDGHVVLARRLEHPRFRRIETISPRNHVHVFRLSQEGEIDADFRAWLAEAYLVGEQR
                     HLDSSRKRA"
     gene            complement(23432..24331)
                     /locus_tag="VNW28_03105"
     CDS             complement(23432..24331)
                     /locus_tag="VNW28_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08943.1"
                     /translation="MPDFKAWFPDLDGFPRPDWKTIRAWIRQNVAPDRLEDAWQAIAR
                     EWLDRVRERLGKSYVVTESTHFQLLSELDERQQRNTLSFLENARARILRQLGDIPLPK
                     RHGKHVILRFTEEDDYYCYISYFDADGEYADSSGTFLSRGYMHIAYPHRERNNADRAT
                     LVHELTHNLLKSFPLPSWLNEALAMGFESDIAGFGGPPLTRELSDEHRAYWNRQTIQE
                     FWRGFSFSQVDGQKLAYSLSRVLLNLIATELRPPPVQFRDFVLHANRQDAGQAAAREY
                     LDVELSDLVATFLGPGDWAPELN"
     gene            complement(24459..>26286)
                     /locus_tag="VNW28_03110"
     CDS             complement(24459..>26286)
                     /locus_tag="VNW28_03110"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HXA08944.1"
                     /translation="ASLLFAALCASPASAQIPRGIFSLGGSGNKASASALADPDVVGI
                     TIRQDWADLEPTEGQFDFSFLDSEVARATAVGKQILLRINTQAAKPAWVTAAIQQAGG
                     TFFTFDNNGVPATIPVFWDPTFLAKKKAMITALGAHLSGNSALRIVAASFANAISEDW
                     SVPHTPPEVLDWFAAGYTSAKLLDAGKQIIDTTMAAFPNQYVTLAIGGNGHVGGTGNL
                     DPTATYVAQNAITTARASWPGRLIAQINSLSTFNPAAPGPSDSAWNLLWNSGPEVAAQ
                     MVYWCYNEPTYRVNGGVPGDPATVLSTSVDAALSYDANYIEVYQTDVLNLPGVITYAR
                     SSLAPSPALPALLNVSTRLQVGLADRVAISGFIVGGTGTKKLLLRAIGPSLTQSGITD
                     PLANPYLELHDQTGAIIATNDNWATTEVGGAIVADQQAEILASTVAPANPAEAAMIVD
                     LSPGAYTAVVRGATNGTGIGLAEVYDLSQSAPATLVNLSTRGFIQTGADVLIGGFILG
                     GSDPSNLIVRALGPSLLQAGISDVLIDPTLDLRDANGTLLASNDNWADTQQAEITASG
                     LAPSDPREAAIEQTLSPGSYTATVSGKSGGIGVGLVEVYKLP"
BASE COUNT         5168 a         8142 c         8138 g         4838 t
ORIGIN      
        1 cgaagagatg ggcttcgccc tgcgccagag agctgagcgg atcggtcacg agccggcccg
       61 cgtagcggag ttgtgggttg atcgtgccat cggaggcgct gaaaccgatg gcgagattgc
      121 cctggccatc catcgccgcg ctgcccatcc agcgcgaagt agtgtccggc tgataggtgc
      181 tttcctggaa gactgtaacc ggcccggcag tcacgccgcg aagctcaaac catcggatgc
      241 ccgcagcgcc gcccgcgttc accgtgaagt tgccaaccaa cgattcgtga tcgccaaagt
      301 tgcgataggc cagccggaac attaaccggt cgccgatgcc atcgagcttg tccgatgaag
      361 tcacacctaa ctccggcacg caggagcgcg tggccggaca gagctgggta aagccggccg
      421 cggcgggagt gttgaagctg gtgaaggtcg cattcgccgg ggtagtgaag tcgacatgga
      481 aatgataggt ggggtaacta ccgccgccgg gaaagccgac gaaggtgttc ggcgcgccgg
      541 cgggcggcgg agtaaaccca tcgaggttgg ccggcaggaa gggcgcgacg gagccaccaa
      601 gtgggccgcc cgggctgatg aacgtggccg gcgcgccagt cagcatcgct gtccggtcga
      661 acgcgaaggg ttgcggacca agataggccg tccctgcggg attgaacaca ttcatgctca
      721 tgtagtaagc gtcgggccag accgagaggt gcggataatc gaagaagttc gagccaaggt
      781 gaaacccata acgactatag gtgccggtgg catcgcccga ggtggagacc gcgacgcatt
      841 catcggtgag cggaatgccg ccggtcgcgc tcgcaaactg gctgatgacc cagcgatcgg
      901 ccagttgatc gtaaaggacg actgggtcgc cgaaaccgcc cgtctcgcaa gcaccgccga
      961 agccgctcca aagcgagacg atgctattcg gcccgaggac ggagttgccc gtggccttgt
     1021 cgaagacctg gtaaccttcg ttcaccatct ggacatattg cgtcaggccg acggcgccgt
     1081 tcgggtcagg cggcgcgcaa ctgcaactta cacccgggaa agcgatgccg tcgaaaccaa
     1141 ggatgggcgc gggaatgctg ggcgcgagga agcgcaggat tgaaccctta tcgataaccg
     1201 ggtcagggcg atcgacatga gagttcggta tcttcgggtt ttcggcggcc tcgcggtcct
     1261 ggtgccgctc agggctatcg gtggcggcca tctcgcgcaa cggaggcgag acgtcattat
     1321 gataggaagg cgtggcgcgc atccgcggtg ggccatgcgc caggaccgcc gtcacggaca
     1381 acacggcgcc cccgcagaaa gcgaggagac gcgcgctcaa gactgagatt ggcctggact
     1441 tcacatccgt cgtcgattgc cccaacgaca gccggtttga aagcagggcc cgttccgcga
     1501 tcgccgcagc gggccgcgtt tgctcgacga gagcgatcgc ctcgcctggc tcggtctccg
     1561 ggtttctccc gaatctagaa aacgccgttg ggcgtcttct tcggcgagag gtagtaaatc
     1621 atgagcccaa gcgcggccgt cgccagcccc cagagcgacg gctcgcgcgt cgagggatct
     1681 tccagcaggt gccagagcat ccagccgctg accgcgagaa agatcgccgg cgtcaccgga
     1741 taaccccagg tgcgatacgg ccgcggcagc gcgggacgcc gccagcgcag gacgaagacc
     1801 ccgaggacgg tgagggcgga gaaaagcgtg aggctaaatt ggacgtagtt cacgactttt
     1861 tgaaacgttg tgaccagaat cagcagattc acaatcgcga attgcaccag cgtcgcgcgg
     1921 atcggaagac cgcgcccgtt gcgccgggac agccacgata acgcgcttag atcttccccc
     1981 atggtcatca tcacgcgcgg cccgatccac atcatggcgc tgaccgtcga aaccaggccg
     2041 agcgcgataa aaacggccat catcttgccg ccatccgtgc caaaaatatg caggcccgcg
     2101 atcaacccga cctcttgttt ccccgccatt tccgcggccg gagtggtgcg cagaaagacg
     2161 gcgttgattc cgagatagag cgccgtgacc aaaagcgtgc cgagcccgag cgagagcgga
     2221 atggtgcgcg tcggattacg cacttcgcct acgatatagg tcgaggcgtt ccaacccgag
     2281 taggcataca tcacccagta aaggctgatc gcgaacggcg gactcaagag caacgcgcgg
     2341 tctgtcttga ccggcaggaa cgagatcggc tgcgaacccc tgacgtagaa tccggcggcg
     2401 atgatcacca aaatcagcgc cactttcaaa agggtggaga tgttttgaaa tccacttccc
     2461 agcttcaaat cccagagcaa aaccagggtc gtgatccaga ctaccgcgag cgacaagaag
     2521 agcggattcg tgcccggcac gacgcccgag atgtaagtcc cgaacggaat cgccgcgatc
     2581 gccaccgggg cagcaaaacc caccgtggcg gagatccagc ccgccacaaa gccgatcgac
     2641 ggatgataaa tttcgcgcag aaaatgatac tcgccgccgg agcgcgggag ggcggcgccc
     2701 agctccgcat aggagagggc gccgcagagc gcgcaaatgc cgcccactgt ccaaagcgcc
     2761 ataatggcaa agccagtcgg caaacccccg acctggaagc cgaggctggt gaaaataccg
     2821 gtgcccacca tgttggccac gacgatgcag ctcgcggtga gcagcgagat cgttctcggg
     2881 ccgtgagcag gagagccggg ttgcaccggc gatgatctag cggtggcgcc tgctgggttc
     2941 aaacgcgaag tcgcgacgtg ctgaagcggc tactcttccg gccagacaaa agggctgcgg
     3001 aaaagaccgc agcccttcgg caccgctggc tttgcctaac gaattattcc gatccggaat
     3061 cgtgtagggt ctccagcgcg tcgagtttct tttgctggtc ggccgtgagc agcgggcgga
     3121 tctgcgtgtg cagttgcttg atgatcggcg cggcctgggc gcgcgcctgt tgatggatgg
     3181 cttccagctg gggctggagt tgcttgatga tcggcgcggc ctgggcgtgc gcctgttgat
     3241 ggatggcttc cagctggggc tggacggcgg cgaccatcgt ttgcacctgg gccttttgcg
     3301 cgtcggtcag gttgagcgcg tggctcaaca tcgcagtcat atccggcggt ccgtggtgat
     3361 gccaggctgg ggcgcagtct ggcgtttggg cgccggcaaa ttgcccgaag gcaagcacgg
     3421 ccgcgctgct gagggtaaag agaatcagtt tcttcgattt catgtttttt gttggttgtt
     3481 agttttttgt ctgcaacagc gctcgctgtt cgattctaca gacacgacga accccggctg
     3541 ggttacaaca tcttccgtga agagttcgcg aaagcgccgc gcccttgtaa cctcttcgtg
     3601 ccagacgtgt cttcagtttg atgtggaccc gctgcgcgat cgcccatgcc ggatgaaaaa
     3661 ttcgatgagc aggcatgcct tcgtcgggtc gcagaggggg acgagcacgc cgcgcgcgag
     3721 cttttgcgac actttcatcc cttcgtcctc aagctggccc gctcgcatct tccacgacgg
     3781 atcagcgagg aggacctcac ccagatgatc ttcattcgga tttttcaaaa cctcgacagt
     3841 tacagcggga aagtgccact cgagcactgg atctcgcggg tcgcagtcaa cacctgtcta
     3901 aatgaattgc gcgccgaaaa gcgacgaccc gaatggcgga tggccgattt cgacgaggaa
     3961 acgtccgccg cgatcgaacg tctcgcggcc atcgagacgg acctcgcctc gcccgacgat
     4021 gcccggcggg cgaaggaact tctcgcccag atgcttgccc aactttcgcc cgaagatcgt
     4081 ctcctcatca cgctcctgca tctcgaggaa cgatcggtcg aggaaatcca tgccttgacc
     4141 ggatggagcc gacctgcgat caaggtccgc gcttttcgag ctcgcgcgaa gatgaagaaa
     4201 atgctccagc aaacagccaa ccgggaaatg attttcaatt aatggacgcc caccagcttc
     4261 tcattcgcat cttctccctt gcgcggcgcg tcccggacgt gccgcctaac gaaatgccgt
     4321 tcggccagga gacggctgtg ctcgcgcact ggcgggaagc gtccgcgcgg cgggcggccg
     4381 gcgcggcttt actgcgcggt ctgcgctggg ccgcgctcac cgcatgtgcc gtcgcggtcc
     4441 tggccgcgat gctggcaaaa gacgagctcg tcgcgtttca gaatcgcttc gacccagaaa
     4501 ctcgggtcgc cgattcggcg atcgtcgacg gctacggtca tgagtaaggc gcgctcgggc
     4561 agagttatcc tttatgcttt gctcatcttc cttgccggcg cggtcaccgg agtgctcgtc
     4621 gcgccgatgg tcggacgcag cttcatacga ccgccgcaag cgaaagacat gtcccaccac
     4681 atgctcgagt acctgcaatc gggcttgaac ttgaccgctc agcaaacggc ccagatcaag
     4741 cctttaatcg aaaaaacggg cgctgacatg gaagtcattc accacgaaac gatgaaacgc
     4801 gtccttgcgc gcctcgcgca gacccacgcc cagatttcag tgctcctgac gcccgaacag
     4861 caggcaaagt ttttgcagat gcaagcagaa caccggaaac aaatgcgaca cgatcatccg
     4921 tttctttcgc cgccgggacc gccgccaccg ccaccgtcgg gaccataggg cagcaattca
     4981 taccgaccgg caaagacgga ccacgtcaac caaccggcga cgggtcgggc gagccaggat
     5041 cacagattcg gcgccccgac ccatcttggc gccactgttt tctcgatttt tctttgaagg
     5101 atcggcgaga ctgcccgtcg aattatttca catgaaaaaa ctgttcatct tgtcgcttgg
     5161 agtcgccgcg agcatggggg catgcgctcc ctatgcctac cctccgcccc cgcctggccc
     5221 gcccggcccg gtcgtaggtg gagtcacggt cgccgtcggg gatcagccct actacgttcg
     5281 tggaccctac tacattgagc atggccgccg ctgggtctgg gtgtccgggc attgggggca
     5341 tcgccatggt cggcaggtct ggatccacgg ccactatcgg gtgcggggtt aacccgacct
     5401 gactcccggt tctcagcccg cctcagcctg caagagagcg gcggagggat tcccaaagtt
     5461 tcgggcgaga tacgactgca aacgcggcgc gttaaagtcc gccgcgcggt agccgacgta
     5521 gccatccggc ctaacgagcg ccaccccgcc ctcgaacagg ccgtaggcgc catgggcgag
     5581 accgctcagg tcatgtaact ccgcctccgc gccggttcgc gcgcgagcga tccggtagct
     5641 gtcgatcgtc ccttccggca tcgtcacgag cacgctacgc agttgctcgg ccggggccgc
     5701 gctcgtcggc gtcgcgccaa gaaagatgag caggacgtgg cgcggttcgc ggaagaggtc
     5761 gaacagccgg cgcgcctgat tgttttcatc gcgcaggtcg gcatcgggcg cgcggtcgcc
     5821 ggctttcaac cggcccgcac catagttttc cacgatcggg ctggtccgat agctcacgtt
     5881 caactcggcc agccggtcgg cgattttttc ttccacaaag tcgatcccgc tcaccatcgg
     5941 aaggagaaaa tcgcgcacgc tttgcgcgac cgaattgcgc atcgtcgcca ggcgcgtgat
     6001 gcggtctgtc agattcaata cgccgctcgc gacgggttcg cgctcgacat gatagctcgc
     6061 gagcaattgc ggaccggcga ttccccggac gacgagggca agtttccagc cgagattgaa
     6121 cgcgtcctga atcccggtgt tcatcccctg cccgcccgcc gggctgtgaa tgtgggcggc
     6181 gtcacccgcg aggaagacgc gcatctgtcg aaactgccgc acctttcgat gcgagatgtg
     6241 gaaacgcgac agccaaaccg gatcgtgcgc gcgaaagctc gcgtcggcac gctgatcaat
     6301 cagttcctga acgtccgcga gacttaagtc cggcaaggtc tggccccgcg tttccggcgg
     6361 aatgttggcg atgatgcggc agcggttttg ctcgaaaaag atgaccccga gcacgccatc
     6421 gttgccgaga taaaggtgca cccggtcaca ggtcagcggc gagtcgagat gcacatcggc
     6481 aaggataaaa ctttcgtcgt attgcgcgcc ctcgaaatgc atcccgaggc tatgccgcgt
     6541 ggtgctgtgc gcgccgtcac aaccgatcag ccacggcgtt tccatttcct cctcgcgacc
     6601 atcggcatgt cgcaacttcg cccgcacggc gtcgctcgtt tgggtcaagc cggtcagctc
     6661 gacattgcgt tccacctcca ctccgcggct cgcgagctgt tcggtcagca agcgttccgt
     6721 ctccgattgt ggaacgccga gcgcgaaggg aaacggggaa gcgaccgagg tcagttcgat
     6781 ctgtgcgatc tgttcggcgc gatgatggag cgagagcccg tggatgcggt gcccgcgttc
     6841 gagaaaacga tcgacgattc ccatcgtcgc gaaggcctcg agcgtgcgcg ggaaaatcgc
     6901 cagtgccctg gacgtttgcg aaggctgcgg agcgcgctcg atgatccggc agcgcactcc
     6961 gtgacgcgcg agttcgttag ccatcgtcag gccaaccggt cccgcgccga caatgagcag
     7021 cgtggaagat tcagctgtca ttggagaaca tatcacatcg cgccggcggg ccgcgcccgc
     7081 cgctcaccac ggccacttct tcggcaactt ctgcttcagg gtctcgaccg aggcgaagag
     7141 atcacccatc ttcccggcgc gggcgacggt ctcgtcgcag cggaaaacga gcagcttcgc
     7201 tttcttcgtc cgggcgcatt cttccacctc tttccacgat accggggtgg aggcggtcgg
     7261 cttgtcccgg gcgcggagcg aataaacgca gatcgtcgtc ttgtgttcgt cgttctggct
     7321 ccaatcgacg aagatttttc ccgggcggag cgctttcgac attctggaaa cgaccaggtc
     7381 gggatgctcg cgctccagat gctccgcgat cgcatgcgat aactccttgg tttgttcgta
     7441 gctgaccgcg gtgttcagcg gaacgtaaac ctggagcccc tttgagccgg acgttttcgc
     7501 ccagctttcc agtttcatgc cctgcagaat ttcccgtagc cagagcccaa cctggcagca
     7561 ctgcatgatg tccgcgggcg cgccgggatc gagatcgaag accatcatcg tggggcgcgc
     7621 cagagccttt ttgcgggaga gcgaagtgtg cagctcgatg tcggcgagat tcgccgccca
     7681 gacgagcgtg ggcagatcct gcgcgaggca aaaatcgatg ttgcgctcat tggattcact
     7741 ccagacccgg gcggtctgca cccacttcgg ccggtgactc gggcaatttt tctcgtagaa
     7801 aaattcacct tcgacgccat ccggatagcg cttcatggtc agcggccggt ccttgagatg
     7861 cggcagaagc acgggggcga tctggatgta gtagttgatc agctccccct tggtgaagcc
     7921 gtccttcggg taaaggacct tgtcgagatt cgaaacctcc aaggtccggt cttcgacgac
     7981 cagttgcgct ttcttgctca ttgttaacca cggattacac ggatttcacg aatgggaaag
     8041 acggcattac cgcctccact ctgagccgtg tcatcgtgtt atccgcggtt aagaaaaaaa
     8101 cctttccggc gagagcatgg gctgcggctg gcagatgcgg ccgacacaat gttcccaagt
     8161 ctcatgccca cttaagatca ggatttccac ccgcagaaaa tagatcggta cttccggcgc
     8221 cgcgagccgc gggaaacgga cggcgtcttt ttccgtcgtc acgatcatgt ccacgtcgcg
     8281 ccggaggcag cgattgataa aactctgcaa ctccggttcg ctataacggt ggtgatcggt
     8341 gaagcgcttg gccagctcga gccgcgcgcc gagtttgcgc aggccatcct caaagctttc
     8401 cggcgcggcg atgccacaga aggaaccgat gtaggtgtca gtcaggcggc cgagcggaag
     8461 ttgttcgccg gtgtagacgt cctgcaagta aagcggctgg tgagcgcact cgatgatctc
     8521 ggcggtgcga ttgtaacgcc ggatgcgcgc gatcaactcg tcgttaggct cgcccgtact
     8581 cttggtgatg aagatgtaac tggcgcgccg cagattgcgc ggcggctccc gcaaagtgcc
     8641 ccgcggaagg agaaactcgt tcccgaacgg cgcctggcga tcgatcagga cgatgtcgag
     8701 ccgatgcttg aggtggagat actgcaggcc gtcgtcgagc aggagcgtat cggctttcat
     8761 ctcccgaatc gcgtgcagtc cgctcttcac ccggtccttg tccacgagca cgatcacgtc
     8821 cttcaagttg ctcgccagca tgtaaggctc gtcgcctgcg gtcagtgaat cgagcaggag
     8881 caattgtccg tctgagacaa cccgcggcgg atcgggcgca acgccgcgaa gccggtcgag
     8941 caaactgcgc ttggagggtt tcgcgacgct tttgtagccg cggctcagga tggcgacgcg
     9001 gcgtcccccg gcctgcagcg cgcgcgcaaa tttttccacg atcggcgtct tgccggtccc
     9061 gccgacggtc aggttgccga tgctgatgac gagacaaccg agcgcccgct cgcggaagat
     9121 acgcttgcga tagaggaaga ggcgcaactg tacaatgcgc gcataaagca gggagaggac
     9181 gtagagcaga gagcgcagga gcgccgcgcg ggcgccgtgt tgacgcccga gaatgacgtc
     9241 gatcgcgaac tgttcgagat tttcaagcca gcggtgcatc gggagtcagg aggaaacaac
     9301 cagccgtacg ccctcgttgt ccgcagtggt gacgcgggtt gcggcctcgc gcaagccgga
     9361 cgcctcccgc attgccgcgg cgacctcgtc gggcgcgcgc agcgtcaggc aggcgatggt
     9421 cgagccggac ccgctcagaa aaccgccgag cgcgccggcc ttctccgccg ccgcgaccac
     9481 gggctcgagg aagggaacaa actttgctcg gaaaggctgg tggaaatgat cgcgcaacgc
     9541 cccgcgcaaa gcttcgtagt tgccgctcgc catcgcggcg gtgatgacac aggcgttggc
     9601 gcacgattcc accgcatcgg cgcgattgat ttcgtcaggc aagacggcgc gcgcctcttt
     9661 tgtttcaacc tcgaagtccg gaatcaggag cacaaagtga agccgcgggt cgaccggaaa
     9721 tttctggtgc cgattgtggc gcgcgacggt gaagccgccg aattcggccg gcgccgcgtt
     9781 atcggggtgc ccttcgagct cggcgcagag accaaaaatc tgcgcgcgcg gcaacgggcg
     9841 atcgagcatg gcgttcaagc cgtggagcac gccaagccgc accgtgacgc tgctcccgag
     9901 gccgcgtgcc cgcggcacct cgcccgtaat ggcgcacgaa aaatggaagg gcgcgcgctg
     9961 cacggcatcg aagaatttac gcgccgcctg tcgcaccatc ccgtcgccgg cgggcgggct
    10021 ggccggcgcg atggtgacga agttataaag ttgcaaagcg accccgaggc aatcgaagcc
    10081 gggcccgaga ttggaggtgg tggcgggaac gcgcaccgtg atctgttgct ttttcaaatg
    10141 aaaacggccc aggatgcact cggaagaggc atggccgctc agattctaac gcgggtcgtt
    10201 cccgcggcaa cattgccgcc cggacgccga tgaacaactg gatcgatttc gggttggtgc
    10261 gggattttgc cgcagagaaa accgaggcct accggctttg cagcaagccc gatgcctggg
    10321 tggaacgctt cggtcccgat gcgctggttt cctaccgaac cgaagccgcg cgcgagcaaa
    10381 tcctgcacga actggatgag tgggcgctgc tcgcgaatca aaaattcgaa cgcgttttcg
    10441 ggcgctatct cccacggcaa aattcggagc gcaatgcgcc gcaattgatt tccggcgacg
    10501 cgagcgtccc gctgcaacgc cacgtgctcg aacgcaacgt ttgttacgcg ctggatttcg
    10561 gcgccggtca ttctgccggg ttgtttcttg accagcggga gaatcgccag ttcgtgcggc
    10621 gcgccgcgcc gcgccgtttg ctcaattgtt tcgcctacac ctgctcattt tctgtgatgg
    10681 cggcgctggc gggcggcgag accctgagcg tcgatctctc gcggaaatca ctcgaccggg
    10741 gccgggataa tttcatcctc aacaaactgg cgaccgaccg tcatcgcttc ctcgttggcg
    10801 acgtcctcac cgtcctcccg cgcctggcgc gcaaagggga atgtttcgac acgatcattc
    10861 tcgatccgcc caccttttcg cgctcgcaca cgggcaaagc gtggcaggtc gagcacgact
    10921 tcgagaagct actcctcgcc gcgctcgaag tggcggagcg cgatgcgaag attttgcttt
    10981 cgaccaactg ctcccgacta accgagcgcg cgctggaggt gatgggccgc ttttgcctca
    11041 aaaccacccg gcgcgccggg agtttcctgg ccccggttgc tcttccggat ttcccacccg
    11101 gggcgggggc gcgctcgctt tggatgatct tgcgttaagc gaagagaggc gaagaatcgc
    11161 cgcggccggg aaatggtcga gtgctttcaa aaacaacgcg ttcccggaca atttcttgag
    11221 cgcaaaatgg tttcgttatg cgaagataag gtcatatgga tagcgaacaa atgacgggcg
    11281 cgaccagcga cctggcggag cgcgggctgc gctacaccag ggagcagtgg gaggaaatcg
    11341 tcgcgcagac ggaggattac gtccgtgcca acccgacgcg gtcgcttctc tacggggtgg
    11401 ctgccggatt catcatcgat cggttgccta tctgccggat cttcggggct ttcgtgcgtc
    11461 tgctcctgat ggccttcaaa ccggccatcc tggtctacgg cgcgatcaag ctttacgagg
    11521 cggtgaaggc cgaagattag tcgttaggcg aaaaagggcg gcgccgcgga gagtcgccta
    11581 gctctcgaga gtgaaaccga ctttgatcgt cacctgccag tgatcgatct tgttcttctc
    11641 gacatagccg cgcgtttcca ccacctcaaa ccaacgcata ttccggatcg ttttgccggc
    11701 ccgggaaagc gcattctgca ccgcggcctc gatgcttttc ggcgacgaac cgaccagctc
    11761 cagcttcttg taaacatgat cgctcatccc ggaaagtcgg ggcgggaggg agagaactca
    11821 aacacaaaac ggcctgggcg ccgagtgcgc gccaagaact ttgcgttgag cggttccgag
    11881 gcgcttgtta gcgtgccttg ctcccatgga aaaaaacatc gagtcccacc tcaaggaaac
    11941 gcgcgtcttc aaaccagcgc gcgccttcgc caaaaatgcg cgcgtcggca gtctcgagga
    12001 ctatcgccgg atgtatcggg aatcgattcg ccaacccgaa aaattctggg cgcgcgaagc
    12061 gcgcgagctg cgctggcaga agccgtggaa gaaagtcctg gagtggaagg cgcctttcgc
    12121 caaatggttc gtcggcggaa aactgaacgt ggcggaaaac tgcctcgatc gtcatctgca
    12181 aggcccgcgc cggaacaagg ccgccatcat ctgggaagga gagcccggcg acaaacgcat
    12241 cctgacttac cagcagttgc atcgcgaagt ctgccgcttc gccaacgtcc tgaagcgcaa
    12301 caaaattcgc aaaggcgatc gcgtcatcat ctacctgccg gcgattccgg aggcggcgat
    12361 cgcgatgctc ggctgcgcgc ggatcggcgc cgtccattcg gttgtcttcg gcggtttcag
    12421 cgcggatagc attcgcgacc gcatcgccga ttgcggcgcg accgcagtca tcaccgcgga
    12481 tggcggtttt cggcgcggcg cgatcgtccc gctcaaacac aacgtcgatg aagcgctcgc
    12541 aggggggacg acgatcaaac gcgtgattgt tttccggcgc gccggcaacg agatccacat
    12601 cgaggaaggt cgcgacgtct ggtggcatcg cgagttggaa tatgtcgacg cgcactgccc
    12661 ggccgtccct ctcgagagcg agcatccgct ctacattctc tacaccagcg gttcgaccgg
    12721 caaaccgaag ggcatcctgc atacgaccgg cggctatctc gtcggcattt attcctccac
    12781 gaaatacgtc ttcgatctgc gcgaagacga catctactgg tgcaccgcgg atatcggctg
    12841 ggtcaccggt cacagctacg ttgtctacgg tccgctcgcc gcgggcgcga ccaccgtgat
    12901 gtatgaaggc gcacccaact ggccggagcc ggatcgcttc tggcgcatca tcgaggaata
    12961 cgcggtcagc atcctttaca cggcgccgac tgccatccgc gccttcattc gttggggcga
    13021 ccagtggttg aagaaacatg atctctcctc gctgcgattg ttaggcacgg tcggcgagcc
    13081 gatcaatccc gaggcctgga tgtggtacca cgagaaaatc ggcggcggcc gctgtccgat
    13141 cgtcgacacc tggtggcaga ccgagacggg cgcgatcatg atcacgccgc tgccgggcgc
    13201 gatcccgacc aagccgggca gcgccacgct cccatttttc ggggtcgacc ccgtggtcgt
    13261 cgatcagcgt ggccgggaag tcgggccgaa cgtgggcggc aaattgatcg ttaggcgtcc
    13321 ttggcccgcc atgctgcgca ccatttatgg cgatagggca cgataccaaa aacaatactg
    13381 gagcgagttc cgaggcaact acctcaccgg ggatggcgcg cgccgcgacc gggatggtta
    13441 cttctggatc gtcggccgca tcgacgacgt cctgaatgtc gctggccacc ggctcggcac
    13501 ctcggaaatc gaaagcgcgc tggtcagcca cgcgaaagtc gcggaagccg ccgcggtcgg
    13561 gcggccggac gaactcaagg gtcagggcgt ggtcgtcttt gtcacactca agagcggagt
    13621 gctgccgaca ccgagcctga aggaagagct gcgcggccac gtgggcacgc acatcggcgc
    13681 gatcgcgaaa ccggacgagg tgcgcttcgc cgaggcgttg cccaaaacgc gcagcggaaa
    13741 aatcatgcgc cgcttgttac gggaaatcgc cgcgggccgg acagtcaccg gcgacatcac
    13801 caccttggaa gatttcagcg tcctggcaaa gttaggcagc cgcgacgaag agtgagtgcg
    13861 cgaccgaaca aatttgtcac cggggcgttg cacccggccg gccggcatcg cgagacttcc
    13921 ccggtgggag cattttttgc ccgccggggc cgacgccccc ggctatagtc cacccgtgag
    13981 ttttatttcc accggattcc gcgagctgac gctgaaagtg cgccggcaac gaacgcggat
    14041 ggcgctgaag cacgaaaagc gtcacctcca aaagtccgag atcgcgctcg gccgtgaagg
    14101 caccgcggag gccgccaagt ttccggaagt gcgcgacgaa atcgtcgcct tgaagaaatt
    14161 cgagcaggaa caaaaagaag tcgcggtccg gatcgccagg atcgaggaag cactcaaaca
    14221 aatcgagtcg caacggcagg aaaattcgcg cgcgcaaaat gccgcgctcg ccaaactcga
    14281 ggaggaaaag cgccccctgg tcgagcgccg cgacgaagcg aaggccgccg tcgagcgctg
    14341 tgaaaaggaa ctcgcgaccg tcgatcgccg cctccaggaa aacgacgccg ccgagcgcga
    14401 gctgcttaaa aaactctcgg ccttgcaggc caccgaacca gcgccggccg atttcgaaac
    14461 gcaaatggaa cgcatcggcg ccgaacgcgc gcgcctcccg cgcgagcaag ccgggctggt
    14521 gcaggcccgc ctcggcagcg tggaagcttc tcgttccgcg cgcgagaaat taacgaccga
    14581 ggaagccgcc gtcgcgcaag ccgacaagaa catcgcccgg gtccgcggcg aattcgaagc
    14641 gcacgagcgc gcgctccagg aaagcagccg cgctcagcag gaggaagtga agcaggcacg
    14701 gcaacaacac cagaccgtcg aggaaaagaa gaatccggct tacttgaaca ttggccgcca
    14761 cctggcctcg caaggcatcg ccccgccgag cgcgccgcat ttgctcacgg aagtgcgcca
    14821 tcgccggggt gccgttgacc gccacgcggc gctccggcag gagctcgccc tgctttccag
    14881 ccagatcgac aaacaagagc tgcgaaaatt ctatttcacc attttctccc tgctcgccct
    14941 cctcgccatc attctcccgc tcatctccca gtcgccgccc aagcgcgatt ggctcccgcg
    15001 agaaacgcgc gccatcgtct cgctcgatat cgatcagtta aacaacggcc cactggccaa
    15061 tcgctggcgc aaagaacagc ccgacttctg gcaaaaagtc gccaccggac tactcggccc
    15121 agccgcgcgc acccccgctc tgaacctcgc gcgcgacgcc cgccgcgtga cccgcgcgct
    15181 gacgatcgag gagaatggcg cgccaggcgc gtatgtcctg gtggagacac gcggcgatct
    15241 cgccccgatc gtgcgcgcca tcacccagga taagagcttt gcgcagagta ccatggcggg
    15301 cctggtcgtc tggcagcgac cggatatcag cgtggcccgc gtcgggccaa aaacgctcgc
    15361 cgtcggttcg ttaggcgaag tcgataaact ggtggaggtg cgactcggca tccaacccga
    15421 tttgaagatc gatgacccgc tgctgaaaag ctttcaggcg ctcgacccgg atagtgcgct
    15481 ccgtcttgtc gccgcggctc cgaccgagct atcgaccttg ctgggcccga ttctgcccga
    15541 tgcattcctc aaatcctgcg ctttgctcgg cctggagctc acgctcgccg cgccggcgaa
    15601 ggctcgttta ctcgttaggg caaccgaggc cgccaaagcg aaggagctgg ccgctggttt
    15661 gcaaaatgaa ccgggccgct ggctgaccat cccgggctcc gacttcgccc tctcggccgg
    15721 gccgccgaaa gtcgagcaaa aaaacgaaaa ccttgacatc cgcttcgacc ttccggaagg
    15781 agcggcgcgg ctcctcctcc agcgcctcgc ccaagtcccg tccgagaccc catgagccgg
    15841 ttcgttaggc ttttccattg gacgttggga gtatcttcgt tctccttctt ctttttcttc
    15901 ctgctcgtcc cccctgccgc catcgccagg ccgacctatg acaacctggt cgatatcaag
    15961 tccgtcgacc cgaccattct cgtcgagttg cgctatgcca cggcgcagaa catcgcgggg
    16021 cacgcgctct acccacccga ttttccggcg ctcgtgcgac cgacaaccgc cgcgcgtctg
    16081 acgaaggcgc aaaaattttt gcgcgcgcgc catctcggac tcaagatctg ggacgcctat
    16141 cggccggtgg ccgcgcagat ggaactctgg cagcgcacgc acaacgggga ttatgtggcc
    16201 gatccttatg acggcaacgg ttcgctgcac acctggggcg tggcggtgga tgcgacgctc
    16261 gtggatgaaa gcggtcggga ccttgccatg ccaaccgggt atgacgagtt cacgcccgcg
    16321 gccaagctgc ggtaccacgg cgacgatccc gtcatcaagt cgcacctgaa aagtctccaa
    16381 gccgcgatgc gcaccggagg cttttatggg acgcccaccg agtggtggca cttcgtggct
    16441 tacgattgga aaaagtacgc gccgatgcgc gaagcgaaac aacttaccag gtaaagagtt
    16501 catgctttcc tcatttctca aggagctcag ccagcccgaa tacgtgcacg ttctgatcaa
    16561 ccctctcccg gtttacggcc ttgcgacggg gatcctcggg ctcatcattg ctctcatcca
    16621 gcgaagccgc cacgcgcagg tcgccgccct cgccctgatt ttgctcagtg cggcgatcgc
    16681 atggcccgtt gtacactttg gcgaggaagg ctacgatcgg gtgctctcga tgtccgacga
    16741 ccaagggcag gcatggctaa aggtgcacga gcatcgggcc aacgagctcg tctggttctt
    16801 ttacgcgctt gcggctgtca cggcgaccgc gatttttgcg cctgcgaaat ggccaaagtc
    16861 cgccatggca ttaaccctcg tcacgctgct tttctccttc gccgttctcg ccctgggtgg
    16921 ctatatcgcc tctgccggcg ggaaaatccg tcaccgcgag ttccgcaacg aaccggcgcc
    16981 gcccgtgcca gccgacgaca acgaccatta acgccgtgcg cttcgacatc atcactcttt
    17041 ttcccgaagc ctgccgggtt ccgttaagcg agagcatgat ggggcgggcg caggaagccg
    17101 gcgcactcga gctgcacatc cataacctac gcgactggac caccgaccgt catcacgtgg
    17161 tcgacgacgc acctttcggc ggtggccagg ggatggtgat gaaaccggaa ccgatcttcg
    17221 ccgcagtcga atcgctccgc accgacgcaa gcacggttgt gctcatgacc ccgcaaggcc
    17281 gacgtttcgc ccaggcgatg gcgcaggatt tctccgccag ggaacatctt attatcgtct
    17341 gcgggcatta cgaaggagtc gatcaccgcg tcgtcgagca tctggttgac gccgagattt
    17401 cgatcggcga ctacgtcctg accaacgggg cgatcgccgc ggtcgtcttc gttgatgcag
    17461 ttgtcaggct gctaccgggt gtccttggcg atgagcagtc agccgcggac gacagcttca
    17521 gcagccgact gctcgaagct ccgcaataca cgcgcccggc ggaattccgc agctggaaag
    17581 ttcccgatgt tttgctctcc ggtcatcacg ccgagatcgc cgcctggcga aagggcgaag
    17641 ccgagcgccg cacccgcgag aaccggcccg atctattctc aaagtaattt tctcatcctc
    17701 gcaaacctgc gatggacgcg ccggtcgccg ccctggaaaa cgctcgatta acctggcgtt
    17761 tgccggctga gaagcacttc cgcgatatcg agcaccctga ggttttcgcc gcccggcgtg
    17821 ccggccatgc catcgctcat catgttcaag caaaacgggc aaccggtcgc gagcgtcttt
    17881 gcgcccgtct cgatcgcttc ctgcgcgcgg agattcgaaa cccgctgctc cggcggttcg
    17941 tcgaaccaca tccgacctcc gcccgcgccg cagcaaaagg tctttttgcc atggcgtggc
    18001 atttcgcgaa actgctcgtc ttttgcagcg tcgagcaccg cgcgcgtcgc atcgacttcg
    18061 ccattcactc gcgcgaggta acacgggtcg tgcaaggtaa ttggttcatc cccgctcgtg
    18121 tcgttagtca gttttcccga cctaatcaat tctgcgagga gcgtcgagtg gtgctgcact
    18181 tcgtactgcc ccccaaactg cgggtattcg ttcttcagcg tgttatgaca gtgtgggcac
    18241 ggagtcacga tcttcctgac cttgcgccgg cccagcgttt cgacattggc ctgggcacgt
    18301 tcctgaaaaa ggaattcgtc gccgatgcgc cgcgccgggt cgccggtgca ggtttcctcg
    18361 ttccccagca ccgcgaaatt cacgccgcct ttctcaagta actgcgccgt cgcccgcgcg
    18421 accttttgcg cgcgcggatc gaaggacgcc gcacaaccga cccagaagag atactcgaag
    18481 ccgggattcg tttcaaccgt tggcacgtcg agcccttccg cccaggccag gcgatcggag
    18541 ttcggacggc cgtaggggtt agcctggttc ccgacctgct gcaaagcgcg cgccggcggt
    18601 ccggaaagtt tgccttcgcc gacgagatag cggcgcatgt cactgatcaa atcgacgtgc
    18661 ccgatgagca cgggacattc ctggacacag gcctggcaca tcgtacagga ccaaagcgtc
    18721 tcggcctgaa tcgtttcatg aacgttgagt ttgccctcgc ctaacgacat taagccgcgc
    18781 aaatcggtca cgaccttctt gggcgacagc ggtttgccgg tcgcggtcgc gggacaggcg
    18841 tcctcgcaac gtccgcattc catgcaggca tcgaggctga gcagttgttg ctgcgtgaac
    18901 tggtcgagct cgctcacccc gatgagtccg gtttcctcga ccgcttgcat ctgcaacggc
    18961 accagcgtgc ccattggtcg ttgggggcgc agggcgatgt tgagcggacc cgtgatgatg
    19021 tgaaggaacc ggttcaccgg cagggtggcg aaaagcaggc cgaccagaat ggtgtgcagc
    19081 caccaggagg ccaggtgcag cgagcgcgct gtctccacgc tgagaccgcg caaaagagtg
    19141 acatcgagca gccacccaat agtcgaccag tgcgccagcc acggttgcac ctgggtgtaa
    19201 tgcagacgca gcccttccac tagaaaaccg gtcagaccga gagccaggag caaactcaga
    19261 agccaccagt cactccggtt gtgaccaagg ctcggtttgc gccggaaagc gcgccgatac
    19321 atcgcgagga gacagccgag gcagaaaacg accccgaaga cgtccatcgt cagttcatag
    19381 atgagatagt accagccgtg atgaaagtag taaggcccgt cgtaggcaat cgagagcagc
    19441 gtggtgccga tggtcagcac aagaaagccg ctgaagagca gcaggtgaag gagcgcgccc
    19501 agtgtctttt tgtggacgcg cttctgcgcc agcccataag cgagcagccg ccgaatccaa
    19561 acccgccagt cccgctcaaa tccgccggtc tgccctttcc gccagtggcg ccagtgagtc
    19621 aggagctgcc acccaagcac ggcgaagctc gccgcgatca tgatgaaaaa caccacgcgc
    19681 atccagggcg cgatgttgcc gaagacctcc cgcgtggtgc tattcacatc gagcgcgatc
    19741 atcggtaaag cgctcagcct cgacgaaccg cttcgatgag ttgcgggacg agctgatgca
    19801 ggtcgcccac caggccatag gtcgccacct gaaaaatcgg cgcgtcggga tccttgttga
    19861 tcgcgacgat gatgcgcgaa tccttgattc cggcgaggtg ctggatcgcg ccggagatac
    19921 ccatcgcgat gtagagctcg ggcgcgacga ccttgccggt ctgtccaacc tgataatcgt
    19981 taggcgccat tccggcatcg accgccgcgc gggtcgcgcc gaccgcgccg ccaagcgcgt
    20041 ccgccaatcc gccgaccagg cgctcgaacg tttccggatc cttcagcggc cggccgccac
    20101 tgaccacgac gcgcgcttcg gtcaaatcgg gccggtcgga gacgcgctgt tcgcgcgaga
    20161 tgaattgcat tccgttaggc aaagacgccg gggcgaagtc gaaccgttcg atggaacagg
    20221 gcgcggcgtc ggccgtcggc gcctcgaagg cggtggcacg cgccgttagc acacagcggt
    20281 cgccttccaa tttcaccgtg gccagcatgc tgcccgcgct cgttggccgg cggtaggcgc
    20341 gcgcgccgtt cacttcttcg agcgcgagca cgtcggtcac catcggtgca tcgagcagcg
    20401 cggccgcgcg cggcaaaata tccttactga aagtcgagga cgagccgaga agagttttcg
    20461 ccccatgctt ttgggcgacc tgcgcaacca ccgcggcgta acgatcggcc agcggttccg
    20521 ccagggccgc gtcgtcggcg acgatgaccg cgctcgcgcc gtaggaaaca agcgacgcgg
    20581 cgacctcatt caggccatga ccaagcacaa ggagtgcata ctcgccgccg agttggcgcg
    20641 cgagcgtgat cgcgtgcaat gaaggaccct tcacctgctg gcgatcgtgt tcgatgagga
    20701 caagcgtcgc ggccatgttc acagcacctt cgcctcctgc tttaacttga ccattaattc
    20761 ttcgaccgat tcgacgcgcg tgcccgcggc gcgttttggc ggacgcgccg tggagagcgt
    20821 aatggtgagc ggctgcacag tcactcccag ttgctcgcag gtcagaactt cgatcggttt
    20881 cttgcgcgcc ttcatcaaac cgggaagcga aacgtagcgc ggttcgttca ggcgcaggtc
    20941 ggtcgtgacg atggcgggca ggttgacgac gatgacctcc aagccgccgt cggtctcgcg
    21001 ggtgcattgc gcgcgggtct tgttctcgag gaactcgagc ttcgagatga acgtcgcctg
    21061 cggccagcct aacaatcccg cgagcatttg gccggcctgg ttggaatcgt cgtccaccgc
    21121 ctgttttccc atgagcacga gctgcggcgc ttctttctcg accagcgcgc ggagaatacg
    21181 cgcgaccgca tagggatcga gggcgcggga atcgtcgatg cgcaacgcgc gatcgacacc
    21241 catcgccagg gcggaacgga tctgttcgtc gactatcgcc ggtccgatgg taacgccgac
    21301 aatttccgtc gcttcgccgc gctcgcgcat gcgcagcgct tcctcgatcg cgatttcgtc
    21361 gaagggattg acgacggatt tcgccgcggc gccgggtccc gcataagggt cgggcacgct
    21421 cttgaacggg acaacgatct tcatacggag ggacgtgctg cggcacgtcc ggaacctcca
    21481 accaagacct ggaggtgcgg aagcacgtcc ctccgaggaa gcaatttgcg gctcgacctc
    21541 ctcacagccc gatgagcgag gcgatgatgt tgcgttggat ttcgctcgtg ccggagtaga
    21601 gcttgctccc gatcgcgtcg cgcagctcgc gctcgacttc gtattcgacc atgtagccgt
    21661 agccgccgtg gatctggatg gcgtcttcgc aacatttcac ccaactctcg ctgatctgga
    21721 gtttcgccat cgctgcttcg aggtaaatgg ctttgcgatg accggcggcc catgcgcatt
    21781 ggtaaagcat gtggcgcgaa ttctcgatcc gcattttcat gtcgacgatc ttgccggcga
    21841 cctgctgaaa tttcccgatc gcctggccgc cctgctggcg ctcgttggcg taacgaatac
    21901 aggtctcgag caaacgctgc atcgtaccga ccgaggcggc caggatgcag ccgcgttccc
    21961 aggtcatgga gcgcgtgaaa agccagctgc ctgcaccttc tttgccaagc aggttttccg
    22021 ccggcacttc gcagtcggtg aaaaagacct cgcccatcgg cgaagtacgc acgcccattt
    22081 tttcgatctt gtgagtgaga tccagtccgg gcgaatcctt ctcgacgagg aaagcgctca
    22141 ggccggaggc gcctttcgtt ttgtcggtcg cggcaaagac gaggagcaca tcggcgaccg
    22201 gaccgttggt cacgaaaatc ttgctcccgt taagaatgta tttgttcccc ttcttgaccg
    22261 ccgtcgtggc gaggctgaac gcatcggagc cgctgttcgg ctcgctcatc gcgttgccgc
    22321 cgatgatgga accgtcgcac aagccgggca gaaatttttt cttctgcgcc tcggtgccgt
    22381 tgtagacgag cgggatgatc gccgtccaca tgtgggcgtt gatcgaaaaa agcaggccgt
    22441 tgtccttgca gccgtagccg agccgctcaa gcgcggcgac ggtcgtgatc gggtccagac
    22501 cgaggccgcc gaattcctcg gaaacgagca ggccatggat gccgaaaccg gcgcacttca
    22561 tccacgcgtc gtgattgaaa acgccgtggc ggtcgttctc gatcatatcg taggcgagct
    22621 gctccttggc gaagcggtcg accgcgtcga gcagttctcg ttgttgatcc gaccaagaaa
    22681 agtccatgag ttgcgttctc cttgagcctg tatccgcttc ttccgacccg atggattcag
    22741 caaggctggt gccgtgtgcc ttgctgggac gcggtaggcg gccattctac aggcgaggga
    22801 caggcggtca agttgacgtc ccggaggatc tgcgcggtgg aattttgtcg gaagtcggga
    22861 cgatcttgct accgtcaaga aaacgccgcc acccttgatc accgtcgcga attcatcccg
    22921 gattaatcga ccgccacgcg cgagacgaac gaccctgcga cccgcactgt ggcgctgccc
    22981 aaaatgccga cgtgcattcg cgaaccgcaa tcaatcgcac gcctgcggcc gtcataagct
    23041 ggccgatcat tttcgcgaca aaccgcggga gattcgagcg ctctatgacc acgtcgtcgc
    23101 cgcgctgcaa gcgatcggcc cggtgcggat tctgcccgaa aagacccgga tcgcgtttca
    23161 agtccggatg tccttcgcgc aagtgacacc gcgacagaag tggttggatg gacatgttgt
    23221 cctcgcccgg cggttggagc atccgcgctt tcgccggatc gagaccattt cgccccgcaa
    23281 ccacgtccac gtttttcgcc tcagtcagga gggcgagatc gacgccgatt tccgcgcctg
    23341 gttggcggag gcctatctgg tcggagaaca gcgccatctg gactcgtcga gaaaacgtgc
    23401 ctaacgacaa caagtcgaac tgtcgccggc gctaattcag ctccggcgcc cagtcgccgg
    23461 gaccgaggaa ggtggcgacg agatcgctca gttcgacgtc caggtactcc cgggcggctg
    23521 cctggccggc gtcctgccgg ttggcgtgca acacgaaatc gcggaattgc accggcggcg
    23581 gccgcaactc agtggcgata aggttcaaga gaacacggga caagctgtag gcgagctttt
    23641 gaccgtcgac ttgagagaag gaaaatcccc gccaaaattc ctgaatggtt tgccggttcc
    23701 agtaagcgcg atgttcgtcc gacagctccc gcgtaagggg cggcccgcca aagccggcga
    23761 tatccgactc gaagcccatg gcaagcgctt cgttcagcca gctgggcagt ggaaaactct
    23821 tcagcagatt atgggttagc tcatggacga gagtcgcgcg gtccgcgttg tttctctcgc
    23881 ggtgtgggta ggcgatgtgc atgtaaccgc gactaagaaa cgtcccactg gagtcggcat
    23941 attcgccgtc cgcatcgaaa taggagatat agcagtagta atcgtcctcc tcggtaaagc
    24001 gcaggatgac gtgtttgcca tggcgtttcg ggagagggat gtcacctaac tgtcgcagaa
    24061 tgcgcgcccg ggcgttctcg agaaaggaaa gggtgttgcg ctgctgcctt tcatccagct
    24121 cggaaagaag ctggaagtga gtcgactcgg taacaacgta ggacttgccc aggcgctccc
    24181 ggactcgatc gagccattcg cgcgcgatcg cctgccaggc gtcttccagc cgatcgggcg
    24241 cgacgttttg ccgaatccat gcgcgaatcg ttttccagtc ggggcgcgga aatccatcga
    24301 ggtcaggaaa ccacgctttg aagtcaggca cctgggagaa catctccgag agtcatctca
    24361 tgaatcccaa aggttgtcat gcgcaccgcg gccctcgctg ccttcggtat cttccgaata
    24421 cattacggcg tgacggcgcg gagtcgtacg ccaaggcgct agggtagctt gtagacttcg
    24481 accaggccga cgccgatgcc accgcttttg cccgagacgg tcgcggtgta gctcccggga
    24541 gaaagcgtct gctcgatcgc agcttcacgt gggtcgctcg gggccaggcc gctggctgtg
    24601 atttcggcct gctgggtgtc ggcccagtta tcgttcgatg cgagcagtgt tccattcgcg
    24661 tcgcgcagat cgagggtcgg atcaatcaag acgtcgctga ttccagcttg caggagggaa
    24721 gggccgaggg cacgcacgat gagatttgac ggatccgatc cgccgaggat aaagccgccg
    24781 ataaggacat ccgcgcccgt ctggatgaag ccacgggtgc taagattcac gagcgtggcc
    24841 ggcgcggact gactcaggtc atagacttcc gccaatccga tgcccgtacc gttggtcgcg
    24901 ccgcggacaa ccgccgtata cgcgcccggg ctcaggtcga cgatcatggc tgcttccgcc
    24961 ggattggccg gggcgactgt cgacgcgaga atctccgcct gctgatcggc gacgatagcg
    25021 ccgccaacct cagtcgttgc ccagttatcg ttggtcgcga tgatggcgcc ggtttggtcg
    25081 tgcagttcga gatacggatt cgccagggga tcagtaatac cggattgagt gagcgagggt
    25141 ccgatcgcgc ggagcagcag ttttttagtt cccgtcccgc cgacaataaa accgctaatc
    25201 gccacccgat cggcgaggcc gacctgtaat ctggtggaga cattcagcag ggcgggcagc
    25261 gcgggcgagg gcgcgagcga gctgcgcgcg taagtaatga cccccggcaa gttcaacaca
    25321 tccgtctggt aaacttcgat gtaattggcg tcatagctca aggccgcatc cacggatgtg
    25381 ctgagaacgg tcgccggatc tcccggcact ccgccgttca cccggtaggt gggttcgttg
    25441 taacaccagt aaaccatttg cgcggcgacc tcgggcccgc tgttccagag gaggttccaa
    25501 gccgaatcgc tcggcccagg agccgcggga ttgaaggtcg aaagactgtt gatctgggcg
    25561 atcaggcgac cgggccagga ggcgcgcgcg gtggtgatag cgttttgcgc gacataggtc
    25621 gcggtcggat caaggttgcc ggtcccgccg acatgaccgt tgccgccgat ggctagagtc
    25681 acatactgat tcgggaaggc cgccatggtc gtatcgatga tctgtttccc ggcgtcgagc
    25741 agcttcgcgc tcgtataacc ggcggcgaac cagtcaagca cttccggagg cgtgtgcggg
    25801 accgaccagt cttcactgat ggcattggcg aaactggccg caacgatcct aagcgcagag
    25861 ttgccgctaa gatgggctcc caacgcggtg atcatcgctt tcttcttggc gaggaaggtc
    25921 ggatcccaaa agaccggaat ggtcgcagga acgccattgt tatcgaaggt gaagaacgta
    25981 ccgcccgcct gctgaatggc agccgtgacc caggccggtt tggctgcctg ggtgttgatg
    26041 cgcaggagaa tctgcttccc cacggccgtc gcccgggcca cctcggaatc gagaaacgag
    26101 aaatcaaact ggccctctgt cggctctaga tcggcccagt cctgccggat cgtgatcccg
    26161 accacgtcgg gatcggccag cgcactcgcg cttgctttgt ttcccgatcc gccaagggaa
    26221 aagatgccgc gcggaatctg cgcacttgcg ggggacgcgc acagggcagc gaaaagcagc
    26281 gaggca
//
LOCUS       DATMNS010000084        12244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1847962, whole genome shotgun sequence.
ACCESSION   DATMNS010000084 DATMNS010000000
VERSION     DATMNS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 12244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12244
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1847962"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /gene="lnt"
                     /locus_tag="VNW28_03115"
     CDS             <1..639
                     /gene="lnt"
                     /locus_tag="VNW28_03115"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXA08945.1"
                     /translation="KNGDYNAALLVSNGAENIQVYHKLHLVPFGEYVPGRHTVPFIAA
                     IVGDQVPGDFTRGKEPVVFRLTTDQVKVAPLICFEDTLGELTRQFVLRGANLLANVTN
                     DGWFLHTAAGRQQLANAVFRCVETRRPMARAANTGVTCFVNRFGRVTQMLLDAKGSQF
                     TEGVLTGAVEVPTAGTLTFYVRHGELFAKGCGGLTLLFLLAQSPRLVRRNSG"
     gene            839..1768
                     /locus_tag="VNW28_03120"
     CDS             839..1768
                     /locus_tag="VNW28_03120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA08946.1"
                     /translation="MDNDSVIFARGLRKSYGTLEAVKGIDFEVSAGEVFGLLGPNGAG
                     KTTTVEILEGLRPRSGGEVKVLGFDPDRQKQALKDRIGVCLQATNLPDKIKVREALEL
                     FASFYTRRVDGDALLRRLQLWEKREAGYATLSGGQKQRLALALALINDPQMLFLDEPT
                     TGLDPQVRLEIRDLIEELRAEKRTILMTTHYIEEAERLCDRVAIIDDGVIIAMGSPSE
                     LQAKSANQSSIEISLGQPLSPGELPAWPDAIRTIVSDDRRKISVSSSRPAKTLVELIK
                     WIDGRGLELDDVHLNRPTLEDVFIELTGKRLRD"
     gene            1772..3970
                     /locus_tag="VNW28_03125"
     CDS             1772..3970
                     /locus_tag="VNW28_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXA08947.1"
                     /translation="MKAFLALLQIDLKLASRNRTVLFFNYFFPLMFFFVFAQLFDANE
                     GSAILQVVTMVIVLGILGNGLFGAGMRAVQEREENVLRRYKVTPITPVPLLAASMITG
                     VLLYLPSVVLVIFLAHQFYGMALPGQLGSLLVFVCLGAIAFRSLGLIIAAVVNSSQES
                     NILIQPIYMAMLFLSGATIPLTYMPHWLQNITQFIPATYLMTGIGGIMQRGETVLANW
                     QAVAALLLTTAVATFIATKLFRWEKEEKLRASAKLWVLVVLLPFLFLGAYQAWSQHDL
                     VKARILARGLRRGQTWLIQNARIFVGNGQVIESGAVLVKNGKIAEVYDGNFPDAKKLN
                     AEAIDAAGKTVLPGLIDMHVHLGAPGGFYDDWSKFDPQKSYERELEAYLYCGITAVRS
                     AGDKVDEMLKLRAQFNSGEKLGTELFLCGPLFTAEGGHGTEYAKRLPEIARAGFNAQF
                     LRIPKTQEEARQQVDELAGKGVNAIKGILEAGVPGYTFNRMNLDLLRAVVEEAHAKKL
                     PTSIHTGDSRDVIDAVALGADSIEHGSFRDEIPDATIVEMTAKGIAFDPTLSVAEGFT
                     DFSKGKSDLLKRSLVQQVATKELLTGTEHAEASAEMKSFREQIGRYPMSLDIGGRDLV
                     KAWHAGVLLVTGSDAGNFLVFHGPTVQREIELWVAAGIPPSVALQAATGNAAKLLRAD
                     DRIGTIEKGKEASLLVVDGNPLQDVRALSSISVVLLKGERVNRATIFEQK"
     gene            complement(4076..4564)
                     /locus_tag="VNW28_03130"
     CDS             complement(4076..4564)
                     /locus_tag="VNW28_03130"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HXA08948.1"
                     /translation="MKFLLSGLATLLLAASAGAEPRVIFLVRHAERADASGPAQADPA
                     LSATGKARAAALVKELRDAGISAIYTSEFRRAQQTASPLANSLGIKIEVVPAKDSAAL
                     LAKLRSASGNVLVVGHSNTVPEIIKALGVSVSVTIAEQDYDDLFLVVSGQPPRLIHLH
                     YP"
     gene            4621..5574
                     /locus_tag="VNW28_03135"
     CDS             4621..5574
                     /locus_tag="VNW28_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HXA08949.1"
                     /translation="MQPITGLHHVTAIAGPAQENLDFYAGVLGMRLVKKSVNQDDPGT
                     YHLFYADAEGHPGTDLTFFPWAQLAPHREGYGLSTEVSLSVPPGSLVFWSDRLGSAGA
                     RVFEIETRFNQRVLPLADPHGLRLSLTESESSLGRPFTPWERSPIPVENQIRGLESAR
                     LVERELEPTVSFLGAGLGFRKIGEEGDWRRYGLGEGKSGQYVDLRAEPGARRGAWGTG
                     SVHHLAWRMRDESQQMEVRQRVAQEGAEPTPVIDRFWFKSVYFREPGGVLFELATDGP
                     GFGVDEEQATLGETLVLPPWLESERAAIEAALPELRGVSFP"
     gene            5695..7011
                     /locus_tag="VNW28_03140"
     CDS             5695..7011
                     /locus_tag="VNW28_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08950.1"
                     /translation="MLTEIDRLTAIREARPAESLFAEKDWLLSPEAFAIEEKHRADLE
                     RLGHQLFVFQRACNQLYQQSVKGRQPGWVARYLDIGKPAELIEFSRQKEFREVLPRVI
                     RPDLVLTDEGWTIAETDSVPGGIGLTAWLNQTYASLGSEVVGGADGMLDGFATVLPRG
                     GDIVISQESATYRPEMEWLVRQLHARFPERSWKVEAAENYQPQTGRDVYRFFELFDLP
                     NLPGITRLIEAAGRGELTVTPPFKPYLEEKTWFALFWLQPLRDFWRRELGDKYFRTLQ
                     KIIPYTWILDPTPLPQHAVIPRLEIQDWREAGKFSQKERDLLLKVSGFSPLGWGSRGV
                     VLGADLPHAEWEQGIADALTNFEREPTIMQRFHKGRLFDQPYWDTETSELKTMHGRVR
                     LCPYYFVEAEGVSLRGALATICPADKKLLHGMSDAILVPSRFTSLA"
     gene            complement(7028..8482)
                     /locus_tag="VNW28_03145"
     CDS             complement(7028..8482)
                     /locus_tag="VNW28_03145"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXA08951.1"
                     /translation="MKIGLLLCLLATSALASDPIVVGSKKFTESYVLGGIAKTVLERA
                     GFAAQERAGMGGTIILWQALRGGQIDVYPEYTGTIGEEILKTKEQLSADEMRNALKKF
                     GVGMSADLGFNNTYALVMRRTEAARLGIQTISDLRRHPDLKVGLTHEFLDRQDGWRPL
                     AATYGLTMPNVSGIDHALGYAALAGGAIDLKDAYSTDAKIQENDLIVLRDDRHFFPEY
                     KAVFLYRLEIDPRAIAALNRFGRIDEVKMISLNAAAERTKDYAYAASLYFHPPPNEGA
                     PKPDLWPKMARWVTRHLQLAGISLFLSVLIGLPLGIVASRGGALGQAILAIVAMIQTI
                     PSLALLALLVPVPFLGISATTAIVALFLYGLLPIVRNTATGLQDISPALRDSAVALGL
                     EPGARLRKIYLPLASRTILAGIKTSAVINIGTATLAALIGAGGLGEPILSGLNLNDHT
                     TILEGAIPAALLALLVQGLFDLLDRFLIPRGLRL"
     gene            complement(8479..9219)
                     /locus_tag="VNW28_03150"
     CDS             complement(8479..9219)
                     /locus_tag="VNW28_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA08952.1"
                     /translation="MVSLVQLIGVSKKFGGATALQPTNLAFPSGLTTVLIGPSGCGKS
                     TLLRLIIALLQPDSGKVIFDGAEVNRATAQAVRRRIGYVIQEGGLFPHLTSRANVLLM
                     SRHLKRPAREMQARLEELCALSQFPRNALERYPAELSGGQRQRVSLMRALMLSPEVLL
                     LDEPLGALDPLVRSALQRDLKEIFTRLKQTAVLVTHDMGEAAYLANEIVLMNEGRIVQ
                     RGSAADLRDHPANEFVSEFINAQRSAAF"
     gene            complement(9309..10280)
                     /gene="egtD"
                     /locus_tag="VNW28_03155"
     CDS             complement(9309..10280)
                     /gene="egtD"
                     /locus_tag="VNW28_03155"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR03438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXA08953.1"
                     /translation="MNGAAGGVSLLDLEPATSDFLEEAIAGLSSSPRTLPSKFFYDER
                     GSDLFQEICELPEYYVTRTETEILRHYGAEMAESIGENAELVGFGTGAGVKTRMLLDH
                     LQNLIAYVPVDISKQRLTESAEALSREMPALEILPVCADYLQPLELPTPSRKPAHIAV
                     YFPGSTIGNMHPEVARHFLRRIARLCGKSGGLIIGVDLQKSSDVLEAAYNDSADVTAA
                     FNLNLLVRANRELDADFDLTAWRHRAIYNQQAARIEMHLISERDQTVHLADREFHFGA
                     GEKIITEFSYKHTVDGFAALAATAGFQLAHVWTDPKKFFAVFHFVTL"
     gene            complement(10277..11527)
                     /gene="egtB"
                     /locus_tag="VNW28_03160"
     CDS             complement(10277..11527)
                     /gene="egtB"
                     /locus_tag="VNW28_03160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HXA08954.1"
                     /translation="MARFREVRNFSKRLCETLEPEDYVVQSMPDVSPTKWHLAHTSWF
                     FETFILKVWMPSYRTAVPQYAYLFNSYYNAAGDMHRRDLRGLISRPTVAETYRFRESI
                     DAHIEELLAGADDELLAELEPVFIIGLHHEQQHQELLVTDIKNVFSHNPLHPVFRPRE
                     KTIESRQNAPAGYVDFAETTLEIGHDGRDFSYDNEGPRHRALVPAFSLATRPVTNGEY
                     LAFIEAGGYTRPEFWLSLGWTTIKDPVSGGWQAPLYWMKQNGQWWNFTLAGMRPVDEA
                     EPVTHVSYFEADAFANWSGARLPTEFEWERAVAGLPIEGNFVDAERFQPAPAAAAPHG
                     ELQQIYGDVWEWTRSAYLPYPGYRAAPGALGEYNGKFMCNQMVLRGGSCATSQNHIRP
                     TYRNFFQPEKRWQFTGIRLARDPA"
     gene            complement(11599..12000)
                     /locus_tag="VNW28_03165"
     CDS             complement(11599..12000)
                     /locus_tag="VNW28_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08955.1"
                     /translation="MPDRSHSPHGKINLHGSGLGVPSPETVEKRAREIAMIDERNPDE
                     FTDSDWHQAKNELTGVETSHVPEMDEDADEEHSERDEVVGASGHRAPKSGFGEDENVG
                     EELVSGGLEEAAHDQMVEARREELEQEREQQ"
BASE COUNT         2331 a         3593 c         3931 g         2389 t
ORIGIN      
        1 aagaacggcg actacaacgc ggccctgctg gtttcaaacg gtgccgaaaa tattcaggtc
       61 tatcacaagc tgcatctcgt gcccttcggc gaatacgtgc ccggccggca caccgtcccc
      121 ttcattgccg cgatcgttgg cgaccaggtg ccgggtgatt tcacgcgcgg gaaagagccg
      181 gtggtttttc gcctaacgac cgatcaagtg aaggttgcgc cgctcatttg cttcgaggac
      241 acgctcggcg agctgaccag gcagtttgtc ctgcggggag cgaacctgct ggccaatgtg
      301 acaaatgacg gatggttcct gcataccgcg gccggacgac agcaactggc caacgccgtc
      361 tttcgctgcg tcgagacgcg ccggccgatg gcccgagcag cgaacacggg ggtcacttgt
      421 ttcgtcaacc gtttcggacg cgtcacgcaa atgttattgg atgccaaagg cagccagttc
      481 accgagggcg tgctgacggg ggcggtggaa gtgccaaccg ccggaacgct gaccttctac
      541 gtgcgacacg gtgaactttt cgccaaaggg tgcggcgggc tcacgttgct tttcctgctc
      601 gcgcaatcgc cacgcctcgt gaggcggaac tcggggtagc gcacgcgtct cgcgtcaacg
      661 aacttttccc aggacggctc ctctttggcg gacgaaagtt gtcaaaaagt ccgcgctggt
      721 gaaacaccag cgccaacacg cgagacgcgt gcgctacccg gaaatcgctg cggcgtcttg
      781 ttcacgatca aacgccaccg ggtcgcgacc ttcggttttc cgtgcatagt ctcgcgcaat
      841 ggataacgac tccgtgattt tcgcccgcgg tctgcgcaaa tcctacggta cactcgaagc
      901 ggtcaagggg atcgacttcg aggtgagcgc gggcgaagtc ttcggtctgc tcgggccgaa
      961 tggcgcgggg aaaacgacca cggtcgaaat cctcgagggc ctccgtccgc gcagcggcgg
     1021 cgaagtgaag gtgcttgggt tcgatcccga ccggcaaaag caggccttaa aagatcggat
     1081 cggggtttgc ctgcaggcga cgaatttgcc cgacaagatc aaggtgcgcg aagcgctcga
     1141 gcttttcgcc tcgttctaca ccaggcgtgt cgacggcgat gcgctcctgc gccgcctcca
     1201 gctctgggaa aaacgcgagg ccggctacgc gacgctctcc ggcggacaga agcagcgcct
     1261 cgcgctcgcg ctcgccctga tcaacgatcc ccagatgctt tttctcgacg agccgacgac
     1321 cgggctcgat ccgcaggtgc gcctcgagat ccgcgatttg attgaggaat tgcgcgcgga
     1381 aaaacgcacg attctgatga ccacgcatta catcgaagaa gcggagcgac tctgcgatcg
     1441 ggtcgcgatc atcgacgacg gggtgattat cgcgatgggt tcgccgtcgg aattgcaggc
     1501 gaaaagcgcc aaccaatcga gcattgagat ttcgttaggc caaccgctct cgccgggcga
     1561 gctgccggcc tggccggacg cgatccgcac gattgtgagc gacgaccggc gcaaaatttc
     1621 ggttagttcc agccgcccgg cgaagactct ggttgagctg atcaagtgga tcgacggccg
     1681 tggcctggaa ctcgacgatg tgcatctcaa ccgcccgacg ctcgaggatg tcttcatcga
     1741 gctgaccggg aagcgcctgc gcgactaacg aatgaaagcg tttctcgccc ttctccaaat
     1801 cgacctcaag ctggcgagcc ggaatcggac cgtccttttc ttcaactatt ttttccccct
     1861 catgtttttc ttcgttttcg cccagttgtt cgacgccaac gagggctcgg ccatcctgca
     1921 ggtggtgacg atggtcatcg ttctcggcat tctcgggaac ggacttttcg gagcgggcat
     1981 gcgcgccgtg caggagcggg aggaaaatgt gctgcgccgt tacaaggtga cgccgatcac
     2041 tcccgtccca ttgctcgcgg cctcgatgat caccggtgtg ctgctttacc tccccagcgt
     2101 ggtgctggtg atctttttgg cccaccagtt ttacgggatg gccctgcccg gccaactcgg
     2161 gtcgctcctc gtttttgtct gcctcggcgc gatcgccttt cgctcgttag gcctgatcat
     2221 cgccgcggtc gtcaattcct cgcaggaaag caacatcctc atccagccga tctacatggc
     2281 gatgcttttc ctgagcggcg cgaccatccc gctcacctac atgccgcatt ggctgcaaaa
     2341 catcacgcag tttattccgg ctacttattt gatgaccggt atcggcggga tcatgcagcg
     2401 cggcgagacg gtcctggcca attggcaggc ggtcgcggcg ctccttctca ccaccgccgt
     2461 cgcgaccttt atcgcgacaa agcttttccg ttgggagaag gaggaaaaac tccgcgcctc
     2521 cgcgaaactc tgggtgctgg tggtgctgct tccgttcctc ttcctcggcg cctaccaggc
     2581 gtggagtcag catgatttgg tcaaggcgcg tatcctggcg cgcgggctcc ggcgcgggca
     2641 gacctggctc atccagaacg cgcgcatctt cgtcggcaac ggccaggtga ttgaatcggg
     2701 cgccgtcctg gtgaaaaacg gaaaaatcgc cgaggtctac gacggcaatt ttcccgacgc
     2761 gaaaaagctc aatgccgagg cgatcgacgc cgccggcaag acggtcctgc cgggcttgat
     2821 cgacatgcac gtccatctcg gagcgcccgg cggattttac gatgattggt cgaagttcga
     2881 tccacaaaaa tcctacgagc gcgaattgga ggcgtatctt tactgcggca tcacggcggt
     2941 ccgcagcgcg ggcgataagg tggatgagat gttgaaattg cgggcgcaat tcaacagcgg
     3001 cgaaaagttg gggaccgagc ttttcctctg cgggccgctc ttcactgccg aaggcgggca
     3061 cggcactgag tacgcgaagc gattgccgga gatcgcgcgg gccggattca acgcccagtt
     3121 cctacgcatc cccaagacac aggaagaagc acgccagcag gtcgatgagc tggcggggaa
     3181 gggcgtgaac gcgatcaagg gaatcctcga agccggcgtc cccggctaca cctttaaccg
     3241 gatgaatctc gatctgcttc gcgcggtggt ggaggaggcg cacgcgaaaa aactgccgac
     3301 ctccattcac accggcgaca gccgcgatgt gatcgacgcg gtcgcgctcg gggcggactc
     3361 gatcgagcac ggctcgttcc gcgacgagat ccccgacgca acgatcgtgg agatgacggc
     3421 gaagggcatc gcctttgatc cgaccttgag cgtggcggag gggttcaccg atttcagtaa
     3481 aggtaaaagc gatctgctca agcgctcgtt agtccaacaa gtcgcgacca aggagttgct
     3541 caccgggacc gagcacgccg aggcgagcgc ggagatgaaa tcgtttcgcg agcagattgg
     3601 ccgttacccg atgtcgttgg acatcggtgg gcgcgacctg gtcaaggcct ggcatgcggg
     3661 cgtccttctc gtgactggct ccgacgcggg caatttcctc gttttccatg gcccgaccgt
     3721 gcagcgtgaa atcgagctct gggtcgcggc ggggattcca ccgtcggtcg cgttgcaggc
     3781 cgcgaccggc aacgcggcca aactgctccg ggccgacgat cgcatcggca cgatcgagaa
     3841 aggcaaggaa gcgtctctcc tcgtggtgga cggcaatccg ttgcaggatg tgcgggcgct
     3901 ctcttcgatt tcggtcgtcc tcctgaaagg cgaacgggtg aatcgggcga cgatcttcga
     3961 gcaaaagtag ctattgcagc atcggaggga cgtgcttccg cacgtcctct tttctcgcgg
     4021 cgggcattct aacagactgg acgtgcggga gcacgtccct ccgagaagga atgccctagg
     4081 gataatgcaa atggatcagc cgtggcggct ggccagagac gacaagaaag agatcatcgt
     4141 aatcctgctc cgcgattgtg acggagacgg agacgccgag cgctttgata atttccggca
     4201 cggtgttgga atgccccacg accaaaacgt tcccggaggc agaccgcaat tttgcgagca
     4261 aggcggcgct gtccttcgcc ggaacgacct cgatttttat gcctaacgaa ttggcgagcg
     4321 gcgacgccgt ttgctgcgcg cgccggaatt cgctcgtgta aatggcgctg atcccggcgt
     4381 cgcgcagttc cttcacgaga gcagcagcac gggccttacc ggtggcggag agggcgggat
     4441 cagcttgcgc gggaccgctg gcgtcggcgc gctccgcgtg gcggacgagg aaaatgacgc
     4501 gcggctccgc gccggcgctt gcggcaagca gaagggtggc caggccagag agcaagaatt
     4561 tcatcaatct gagccacgat aacgcttttt cttcgggagc aagttgcgcg agattgcttg
     4621 atgcaaccga tcacaggact tcatcacgtc acggccatcg ccggaccggc gcaggagaat
     4681 ctggatttct acgcgggggt gctcgggatg cgactggtga agaagagcgt gaaccaggac
     4741 gatcccggca cctaccattt gttctacgcc gacgccgaag gacatcccgg aaccgacctg
     4801 actttctttc cctgggcgca actcgcaccg caccgggagg gttacggatt gagcacggag
     4861 gtgtcgctct ccgtgccgcc gggcagcctg gtcttttgga gcgaccggct aggaagcgcc
     4921 ggggcacggg tgttcgagat cgaaacgcgg ttcaatcaac gcgttctgcc attggccgat
     4981 ccacatggac tgcgactcag tttgacggaa agtgagagtt cgttagggcg accgttcacc
     5041 ccctgggagc gcagcccgat cccggtggaa aatcagattc gaggattgga gagtgcgcga
     5101 ctggtggagc gcgagctgga gccgaccgtt tcctttcttg gcgcgggact cggattccga
     5161 aagatcggtg aagagggcga ctggaggcgc tacggtttgg gtgaagggaa gtcgggacaa
     5221 tacgtcgatc tgcgcgccga gccgggggcg cgtcgtggcg cgtgggggac cggcagcgtc
     5281 catcatctcg cctggcgaat gcgggatgaa agccagcaga tggaagtgcg gcagcgggtc
     5341 gcacaagagg gtgcggagcc gacgccggtc atcgaccgct tctggtttaa atcggtctac
     5401 ttccgcgaac cgggtggcgt ccttttcgaa ctggcgaccg acggtccggg tttcggcgtc
     5461 gatgaagagc aggcaacgct gggggaaaca ctcgttcttc ctccctggct tgaatccgaa
     5521 cgcgcggcga tcgaagcggc gctcccggaa ctccgcggcg tttcttttcc ctaacgaaag
     5581 agacgaggca ggcgcgctct ccgtcgcgct tcctgcaccc gggccgggat tttctcgaat
     5641 cctggttgga cgtgacacgg ccgcgcgttg aaagttgccg ggccgcacca ttccatgctc
     5701 acggaaatcg atcgtctcac cgccatccgc gaggctcgtc cggccgagag cctctttgcc
     5761 gaaaaagact ggctcctctc gcccgaggcc tttgccattg aggaaaagca tcgcgccgat
     5821 ctcgagcggc tgggccatca gctttttgtt tttcagcggg cctgcaatca actctaccag
     5881 cagagcgtca aggggaggca gccgggctgg gtcgcgcgtt atctggatat cgggaaaccg
     5941 gcggagctga tcgagttttc gcgccagaag gaatttcgcg aggtgctgcc gcgcgttatc
     6001 cggcccgatc ttgttctgac tgacgagggc tggacgatcg cggaaacgga ttccgtgccg
     6061 ggcgggatcg gattgaccgc gtggttgaat caaacctacg ccagcctcgg gtccgaggta
     6121 gtcggcgggg cggacgggat gctcgacggc tttgccaccg tcctgccgcg cggcggcgac
     6181 attgtcatct cgcaggaatc ggccacctac cgaccggaga tggaatggct cgttaggcag
     6241 ctgcacgcgc gcttcccgga gcgttcctgg aaagtcgagg cagccgaaaa ctatcaaccg
     6301 cagaccggtc gcgacgttta ccggttcttc gagctgtttg atctgccgaa tttacccggt
     6361 attaccaggc tgatcgaggc cgccgggcga ggcgagctga ccgtcacgcc gcccttcaaa
     6421 ccgtacctcg aagagaaaac gtggttcgcg cttttctggc tgcagccctt gcgggatttc
     6481 tggcgccgcg agctgggcga caaatatttt cggaccctgc aaaagatcat tccctacact
     6541 tggatcctcg atccgacgcc tttgccgcaa cacgcggtca ttccccggct cgagattcag
     6601 gattggcggg aggccgggaa gttcagccag aaagagcgcg atcttctgct gaaggtgagc
     6661 gggttctcgc cgttaggctg gggcagccgc ggggtcgtcc tcggggccga tctcccacac
     6721 gcggaatggg agcagggcat cgcggacgcg ctgaccaact tcgagcgcga gccgacgatc
     6781 atgcagcgct ttcacaaggg ccgcctgttt gatcaaccgt attgggacac ggagaccagc
     6841 gagctcaaaa cgatgcacgg ccgggtgcgg ctctgtccgt attattttgt ggaagccgag
     6901 ggtgtgagtc tgcgcggcgc gctggccacc atctgtccgg cggacaagaa gcttctccac
     6961 ggcatgagcg atgcgattct tgtcccgagc cggtttacga gtttggccta acgagtgtgc
     7021 ggggggtcta cagccgcaac ccgcgcggga tgaggaaacg atcgagcaga tcgaacaatc
     7081 cctggaccaa aagtgcgagc agcgcggcgg ggattgcccc ttcaagaatc gtcgtgtgat
     7141 cgttaaggtt cagtccgctc aggatcggtt cgcccaagcc tccggcgccg atcaacgccg
     7201 cgagggtggc ggtcccgata ttgatgaccg cgctcgtctt gatgccggcg agaatggtgc
     7261 gcgacgcgag cggcaaataa atcttgcgca gccgcgcgcc cggctcgagc ccgagcgcga
     7321 cggcggaatc gcgcagcgcg ggagagatat cctgcaatcc ggtggccgtg ttccgaacga
     7381 tcggaagcaa tccgtaaaga aaaagcgcga caatcgcggt cgtcgcgctg atgccgagga
     7441 agggcacagg gacaaggagg gcgaggaggg cgagggaagg aatggtctga atcatggcga
     7501 cgatcgccag gatcgcctgg ccgagcgcgc caccgcggct ggcgacaatg cctaacggca
     7561 aaccgatgag cacggagagg aagagcgaga tgccggcgag ttgaagatgc cgggtaaccc
     7621 agcgcgccat ctttggccag agatcgggtt tgggagcgcc ctcgttaggc ggaggatgaa
     7681 agtagagaga ggcggcgtag gcgtagtcct tcgtccgctc cgcggccgca ttaagcgaga
     7741 tcattttgac ctcatcgatc cggccgaagc gattgagcgc ggcgatggcg cgcggatcga
     7801 tctccagccg gtagagaaaa acggctttgt attccggaaa gaagtgccgg tcgtcgcgca
     7861 gcacaatgag atcgttctcc tggatcttgg cgtcggtgga atatgcgtcc ttgagatcga
     7921 tcgcgccgcc ggcgagcgcg gcgtagccga gggcgtggtc gatgccgctg acgttaggca
     7981 tcgtcaggcc gtaagtcgcc gccagcgggc gccagccgtc ctgccgatcg aggaattcgt
     8041 gggtgagacc gactttgagg tctgggtgcc ggcgcaggtc gctgatcgtt tgaatgccga
     8101 gtctcgccgc ttccgttcgc cgcatgacga gcgcgtaggt gttgttgaaa ccaagatcgg
     8161 ccgacatgcc gactccgaat ttcttcaatg cattgcgcat ttcgtcggcc gacagctgct
     8221 ccttcgtctt cagaatttct tcgccgatcg tgccggtgta ttccgggtaa acgtcgatct
     8281 gtccgccgcg gagggcctgc cagagaatga tcgtgccgcc cattccagct cgctcctggg
     8341 cggcgaagcc ggcgcgttcc aggacggtct tcgcgatccc gccgagaacg taggattccg
     8401 tgaatttttt tgacccgacc acgatcggat cagacgccag cgccgaggtg gcaagcaggc
     8461 agaggagcag tccgattttc aaaacgcggc gctcctttgc gcgttgatga actcggagac
     8521 aaactcgtta gccggatgat cgcgcaggtc ggcggcgctg ccgcgctgga cgatgcgccc
     8581 ttcgttcatc aggacgatct cgttggccaa ataggcggct tcccccatgt cgtgcgtgac
     8641 gaggaccgcg gtttgtttca ggcgcgtgaa aatttccttc agatcacgct gaagcgcgga
     8701 cctcacgagc gggtcgagcg cgccgagcgg ttcgtcgagg aggagcacct ccggcgaaag
     8761 catgagcgcg cgcattaggc tgacgcgctg gcgttgccca ccggagagtt cggccggata
     8821 acgctcgagc gcattccgcg gaaactggga gagcgcgcag agttcctcca gccgcgcctg
     8881 catctcgcgc gcgggccgct tcaaatgtcg cgacatgagg aggacgttgg cccgcgacgt
     8941 gaggtgggga aataaaccgc cctcctggat aacgtagccg atccgacggc gaaccgcctg
     9001 cgcagtggcg cggtttacct ctgcgccgtc gaaaatcact ttaccgctgt ccggttgcag
     9061 gagggcgatg atgaggcgga ggagcgtcga ttttccgcag ccgctcgggc cgatgaggac
     9121 ggtcgtgagg ccagatggga aagcaaggtt ggtcggctgg agagcggtgg cgccgccgaa
     9181 cttcttgctc acgccgatca gttgaacaag tgaaaccatg ggcgaacgcg ccaagactgg
     9241 aacttgtagc cgggggcgtt gacctcggca acccggttgt ggtcatccgc cgggatcacc
     9301 gctcccggct acaacgttac aaaatgaaag acggcgaaga attttttcgg atcagtccag
     9361 acgtgcgcga gctgaaagcc ggccgtggcg gcgagcgcgg cgaagccgtc tacggtgtgt
     9421 ttgtaggaaa attccgtgat gattttttcc ccggccccga aatgaaattc ccggtcggcc
     9481 agatgaaccg tctggtcgcg ctcgctgatc agatgcatct cgatccgggc cgcctgctgg
     9541 ttgtagatgg cccgatgccg ccaggccgtg aggtcgaaat cggcgtccaa ttcgcgattt
     9601 gcgcggacca gcaggttgag attaaaagcc gcggtcacgt ccgcgctgtc gttgtaggcc
     9661 gcttccaaaa cgtcggatga tttttgcaga tcgactccga tgatcaaccc gccgctcttg
     9721 ccgcagagcc gcgcgatgcg ccgcagaaaa tggcgcgcca cttcaggatg catgttgccg
     9781 atggtcgaac cagggaagta gaccgcaatg tgggccggct tgcgggaagg cgtcggcaat
     9841 tccagcggtt gcaggtagtc ggcgcagacg ggcaggattt cgagcgccgg catctcgcgg
     9901 ctgagggctt cggccgattc cgtcagacgt tgcttcgaga tgtccaccgg cacatatgcg
     9961 atcagattct gcagatgatc gagcagcatc cgcgtcttca cgccggcccc ggtgccgaag
    10021 cctaccagct ccgcattttc accgatcgac tccgccatct cggccccgta gtggcggagg
    10081 atttcggttt cggtccgcgt gacataatat tcgggcagct cacagatttc ctgaaagaga
    10141 tcggagccgc gctcgtcgta aaagaacttg ctggggagcg tgcggggcga gctggaaagg
    10201 cccgcgattg cttcctcgag gaaatcggaa gtcgctggct cgagatcgag gagtgagaca
    10261 ccgccggccg cgccgttcat gccgggtcgc gggcgagtcg gatgccggtg aattgccagc
    10321 gtttttccgg ttggaaaaaa ttgcggtagg tcggccggat gtgattttgc gaagtcgcgc
    10381 aagagccgcc gcgcaacacc atctggttgc acatgaattt gccgttgtac tcgccgagcg
    10441 ccccgggcgc ggcgcggtag ccggggtagg ggagataggc gctgcgggtc cattcccaga
    10501 cgtcgccgta aatctgttgc agctcgccgt gaggcgcggc ggcggccggg gcgggttgaa
    10561 aacgttcggc gtcgacgaag ttgccttcga tcggcaagcc ggcgacggcg cgctcccatt
    10621 caaactcggt cggcaaacgg gcgccgctcc agttggcaaa agcgtccgct tcgaaataac
    10681 tcacgtgggt aaccggctcg gcttcgtcga ccggacgcat cccggcgagc gtgaaattcc
    10741 accattgacc gttttgtttc atccagtaga gcggcgcctg ccagccgccg gaaacgggat
    10801 ctttgatcgt ggtccagcct aacgagagcc agaactccgg ccgggtgtag ccgcccgctt
    10861 cgatgaaggc gagatactcg ccgttcgtca ccggccgggt cgcgagcgag aaagccggca
    10921 caagcgcgcg gtgccgagga ccctcgttgt cgtagctaaa gtcgcgaccg tcgtgtccga
    10981 tctccagcgt cgtctcggcg aaatccacgt agcccgcggg agcgttttgt cgcgactcga
    11041 tcgttttctc ccgcggccga aaaaccggat ggagcgggtt gtgcgagaaa acgtttttga
    11101 tgtcggtcac gagcagttcc tggtgctgct gttcgtgatg caggccaatg atgaagaccg
    11161 gttccagttc cgcgagcagt tcatcgtctg cgcctgcgag caactcctcg atgtgggcgt
    11221 cgatcgactc ccggaagcga taggtttcgg ccaccgtcgg acgcgagatc agcccgcgca
    11281 ggtcccgccg atgcatgtcg cccgccgcat tgtaatagga gttaaaaaga taggcgtatt
    11341 gcgggaccgc ggtgcgatag cttggcatcc acaccttcag gatgaaggtc tcgaaaaacc
    11401 aactggtatg cgcgaggtgc catttcgtgg ggctgacgtc gggcatcgat tgcacgacgt
    11461 aatcctccgg ttcgagggtc tcgcacaggc gtttggaaaa attgcggacc tcgcgaaaac
    11521 gggccaacaa ccttcccgcg cgtttttctt ctctgggcaa cggctgcgtc tcggccggcg
    11581 caatgagtga cgcgctattc attgctgctc gcgctcctgt tccaattctt cgcgccgcgc
    11641 ttcgaccatt tgatcgtgcg cggcctcctc gaggccgccg ctgaccagtt cttcgccgac
    11701 attctcatcc tctccgaaac cacttttcgg cgcgcggtga ccggaggcgc cgacgacctc
    11761 gtcccgttcg ctgtgttcct cgtctgcgtc ttcgtccatt tcgggcacgt gggacgtttc
    11821 cacgccggtc agttcgtttt tcgcctgatg ccaatccgaa tcggtgaact cgtcgggatt
    11881 tcgttcatcg atcatcgcga tctcgcgcgc tcgcttctcg accgtctcgg gcgagggaac
    11941 gccgagtccg cttccgtgaa ggttgatttt cccgtgcggg gagtgcgatc gatcaggcat
    12001 aactggtaat tcgcttggca gaccccaaac gttcgcatcg aagcgccgtt tctgcatctc
    12061 gggtcttttt ctttggtatg agaattcgaa ttttcatctc aacccaccgg attcggggaa
    12121 ccaacgtcgc cgccaggcac gatcgatctg gccgggcaat cgccggcagt ggcccgccct
    12181 gtgttagtga ggttttgatt gacatcgatc atccgaaaat agtagcctga ttcaacgtcc
    12241 caag
//
LOCUS       DATMNS010000085         8168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3639639, whole genome shotgun sequence.
ACCESSION   DATMNS010000085 DATMNS010000000
VERSION     DATMNS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3639639"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..979)
                     /locus_tag="VNW28_03170"
     CDS             complement(380..979)
                     /locus_tag="VNW28_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08956.1"
                     /translation="MKSASFIALLVIAWFAYERMTAPLTYPPGVLIASEPLQTDATPN
                     DAPFEDGKFHLKPLAHFALDARVLHRKIYRYDRQAALVPVDLAVGWGPMSNQAVLDQL
                     EISQSARFFWYEWQHQPPIAPEEIISHATNLHLIPSSKGLESQCKSLRAGELIHLSGL
                     LVEATGPEIGTWRSSLTRTDTGNGACELVWVEEIHKLAR"
     gene            complement(1118..1861)
                     /gene="fabG"
                     /locus_tag="VNW28_03175"
     CDS             complement(1118..1861)
                     /gene="fabG"
                     /locus_tag="VNW28_03175"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HXA08957.1"
                     /translation="MRFENQVAVVTGAGRGIGHAIAVRLAGEGARVAAVSRSETNARR
                     TADEINASRADAAKAYAVDVADHSAVQATAARILEDFGRIDILINNAGVTRDGLSLRM
                     SIEDWETVIDTNLKGAFSFTQAIMRSMIKQRSGRIINISSISGLTGNAGQANYAASKA
                     GLIGLTKSLARELASRGITVNAVAPGFIMTDMTDVLPEPVKQAILGKIPLGKFGEGED
                     IAAAVAFLAAPEAKYITGQVLTVDGGMVM"
     gene            complement(1982..3313)
                     /locus_tag="VNW28_03180"
     CDS             complement(1982..3313)
                     /locus_tag="VNW28_03180"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXA08958.1"
                     /translation="MKRVFFLLRAICALSGLTSALAADLPPVTIALKAARLFDGKTKT
                     LLPKGVVIVTGNTITAVGSNLPIPGDAQIIDLGDATLSPGFMDAHTHLTADYSGNYNE
                     RRLRSLDENVSQIALETIPHARATLEAGFTSVRDLGSRIPNSHDFPDVSLRNVINEGL
                     IPGPRMLVATHGIGATGGHFDDTNGFRDLLFGAEADWSNGIADGPDAIRKAVRYEVKN
                     GADVIKAAVSGGVLSLGDEVDVPQLDPAEMAALVDESHRLRKKVAVHCHGDQAAREAI
                     NAGVDSIEHGSFLKPDTLVLMKQKGTYLTPTLMASEWVMSKIGQYPPAVQAKGRAATA
                     ARSEMFRNALRIGVKISFGTDAAVFPHGMNAKEFALMVGLGMKPIDALLSATSVDAEL
                     LGVASKLGTLEKGKLADVIAMPGDPTADITATERVFFVMKDGKIVKNEAKP"
     gene            complement(3376..3921)
                     /gene="thpR"
                     /locus_tag="VNW28_03185"
     CDS             complement(3376..3921)
                     /gene="thpR"
                     /locus_tag="VNW28_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXA08959.1"
                     /translation="MRAKRLFVSLELPESVAELLVRLNPHRRGIRWLSAEQIHLTLGF
                     LGDVAADAEEKLRPELSAIRFTAFFLPVCGLGVFPGKGRPKIIWIGVGAGHPHLFQLH
                     KRVTDAVLGAGLEADLRPWHPHLTLARCQDVSAESIRPFLRAQADFDAGLVRVDSFQL
                     KSSLLLPDGSIYETELVVPAL"
     gene            complement(3931..4944)
                     /locus_tag="VNW28_03190"
     CDS             complement(3931..4944)
                     /locus_tag="VNW28_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXA08960.1"
                     /translation="MKRSLLLTVGGIGCATWAVSRLLRNDFSFAGKSILITGGSRGLG
                     RTIARRIAAEGGRVALLARDADELARACAELRALGGEALAVPCDLLDRGQTLGAIETV
                     VDRFGGIDVLINNAGIIEVGPLENMRRQDFEKSMQLHFWAPFNLIRQVIPHMRRAGGG
                     RIVNIASIGGKVAVPHLAPYSASKFALVGLSDAFRAELALEGIHLTTVTPGMMRTGSQ
                     ANATFKGDHGAEFTWFSLSTALPFAAVNAERAAEKIVAACRRGQSSLIIGWPARVAII
                     GNAIFPSLTGEFMKLVNRMLPGPVGVEGDELKSGRQARVAQRVPGWLSRLGDVAIGRR
                     HET"
     gene            complement(5003..6217)
                     /locus_tag="VNW28_03195"
     CDS             complement(5003..6217)
                     /locus_tag="VNW28_03195"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXA08961.1"
                     /translation="MSPLFLIFLTVFIDMVGFGIIIPVLPLYAEHFHATPVQIGWLTG
                     IYSGMQIVFVPILGRLSDRFGRRPVLIVSLAGTALGFLIMGWASALPLLFVARIIDGA
                     SGGNISTAQAYIADISTPENRSRSMGVIGAAFGLGFTFGPMIGGIMSHISYGAPFYFA
                     AALAAVNVVLLYFILPESLSPEYRSQPHKRSGLAEVFRHGHARMFGTIVATYFFHITG
                     FAIMTTLFALFTEKHFGFDARQTGYVFGFIGIISVILQGGLIGRLIRFFGETALARTG
                     LLLLAVAMAFLPFVSTVPLLLLACAALAIANGLVNPTLNGLASQMIDRSWQGRALGLM
                     QSAGSSGRLIGPLIGGWLLMFDLGRPLTRYARTPFFASALILLVAFTLAFSVRKPSAD
                     RTPEATPVEADV"
     gene            complement(6406..6867)
                     /gene="smpB"
                     /locus_tag="VNW28_03200"
     CDS             complement(6406..6867)
                     /gene="smpB"
                     /locus_tag="VNW28_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXA08962.1"
                     /translation="MPAETILNRKALRDFHILERFEAGVELKGSEVKSIRAGKANIGD
                     AFARIEKDEAFLYGADIQPYERASHEVPAPKRVRKLLLHRREIDKLYGLTNIAGRTLV
                     ILKLYWKNGRIKAEIGVAQGKEARDKRLDLKKRATDRETAREVARFNRKHG"
     gene            complement(6968..7183)
                     /locus_tag="VNW28_03205"
     CDS             complement(6968..7183)
                     /locus_tag="VNW28_03205"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXA08963.1"
                     /translation="MTAQLLLEAAKVIPNQQLLINVVSKRVRQLGIGHRPLVEATPKS
                     SLTDIALKEIIAGKLTYEELKDTPAGA"
     gene            7447..7520
                     /locus_tag="VNW28_03210"
     tRNA            7447..7520
                     /locus_tag="VNW28_03210"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7481..7483,aa:Pro,seq:cgg)
     gene            7583..>8168
                     /locus_tag="VNW28_03215"
     CDS             7583..>8168
                     /locus_tag="VNW28_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08964.1"
                     /translation="MTDSVLTTIAVTGFTIAFFHAAIPTHWLPFVLTARAQKWSRPKT
                     LAVTAVAASGHATFTASLGLIVAWLGIALSDRVGIWFPRIAGGALILFGLVYVIRQLT
                     GHTHTHLHLGQAHSHDHHHEGHDHGGKRLSLSKSDLAAITSLLALLTFSPCEAFVPIY
                     VSGVRYGWPGFALLTIILTVATIAGMVAFTALALT"
BASE COUNT         1595 a         2512 c         2538 g         1523 t
ORIGIN      
        1 cttccgcatg gccggtagca tggctgcaga catcttcgta agtcggattc tcggtcgtgc
       61 cgccggcgta gccgctcgtc gcggtgatca ctcccttggt gttacgaaat aattcctcga
      121 cgccccaaaa acaaccggcg ccaaaagtcg ctttttcgat gctcatgggc tcaccatcaa
      181 acgagagggc cagccacgca aacgagaggc ggtcctcctg gagggacggg ctctgtcccg
      241 tccgtgtttc agaatcccgg acgacacccg ccgcggcggg caggctctgg tcgtccctcc
      301 agcgcattct ccggtcggtc cagcgcttga ttacggcgac actccaaccc gcacttcgca
      361 gagcgaagtg gccgcaacct cagcgcgcca gtttgtggat ctcttcaacc cagaccagct
      421 cgcaggcgcc gttcccggtg tcggtgcgag tgagtgagct gcgccaggtg ccgatctccg
      481 gcccggttgc ttcaacgagc aggccggaga gatgaatcag ctcaccggca cggagcgatt
      541 tgcattggga ctccaaccct ttgctcgacg ggatgagatg gaggttggtc gcgtgcgaga
      601 tgatctcttc gggagcgatc ggcggctggt gctgccactc ataccagaaa aaacgtgcgc
      661 tttggctgat ctccagttga tcgagcaccg cctggtttga catcggcccc cagccgaccg
      721 cgagatccac cggcacgagg gcggcctggc gatcgtagcg atagattttt cgatgcaaca
      781 cccgcgcgtc gagggcgaaa tgggccaggg gtttgagatg gaactttcca tcctcgaagg
      841 gcgcgtcgtt aggcgtggcg tccgtctgca gtggttcgct ggcaatgagg acgccgggcg
      901 gataggtgag tggcgcggtc attcgttcgt aggcgaacca ggcgatgacg aggagcgcga
      961 taaaggaggc ggatttcacg gtgggataac atatgagcag cagatttggt gctacctgta
     1021 ggcgcttcgc tgtgcgaagc gcggggaaat gactgctgct cccgggactc ttcgttgcga
     1081 gctaaatccc gcgcttcgca cagcgaagcg gctacaacta catcaccatt ccgccatcga
     1141 ccgtcagcac ctggccggtg atgtatttcg cttccggcgc ggcgaggaac gcgaccgccg
     1201 cggcgatgtc ttcgccttcc ccgaatttcc cgagcgggat tttccccagg atggcttgct
     1261 tcactggctc cggtagaacg tcggtcatgt cggtcatgat gaagccgggg gcgacggcgt
     1321 tcaccgtgat gccgcggctc gccagttcgc gcgcgagcga tttcgtcagg ccaatcagcc
     1381 cggctttgct cgccgcataa ttcgcctgcc ccgcgttgcc cgtcagcccg ctgatcgagc
     1441 tgatattgat gatccgtccg ctgcgctgct tgatcatcga acgcatgatc gcctgggtga
     1501 aactgaacgc gcccttcaag ttcgtatcga tgacggtctc ccagtcttcg attgacatcc
     1561 gcaggctcag tccgtcgcgc gtcacgcccg cgttattgat cagaatatcg atccgcccaa
     1621 agtcttcgag aatgcgcgcc gcggtcgcct gtaccgcgga gtgatcggcg acatcgaccg
     1681 cgtaggcttt ggcggcatcc gcgcggcttg cgttgatttc atcagcggtc cgtcgcgcat
     1741 tcgtctcgct ccgactgacc gcggcgaccc gcgcgccttc gccggccaga cgaacggcga
     1801 tggcatgacc gatcccccgc cccgctcctg tcacgaccgc gacctgattc tcgaatctca
     1861 tatgcagaat atcctaaccg cagagattgc agaggacgca gaggaaaaca aaattattgt
     1921 catcgatcct cctctctgcg cgctctgcct cctcagcggt tagcctaacg acaccaacat
     1981 ctcacggctt ggcctcgttc ttcacgatct tgccgtcttt catgacgaag aagacgcgct
     2041 cggtcgcagt gatgtccgcg gttggatcgc ccggcatcgc gatcacgtcg gcgagcttgc
     2101 ccttttccag cgtgccgagc ttgctcgcga cgccgagaag ttcggcgtcg accgaagtcg
     2161 cgctgagcaa cgcgtcgatt ggcttcatcc cgagaccgac catgagcgcg aattccttcg
     2221 cgttcatccc gtgagggaag acggcggcgt cggttccgaa cgaaatcttc acgccgatcc
     2281 tgagcgcgtt acgaaacatc tccgagcgcg ctgcggtcgc cgctcttccc ttggcctgaa
     2341 ccgcgggcgg gtactggccg atcttgctca tgacccactc cgacgccatc aaagtggggg
     2401 tgagataggt ccccttttgc ttcatcaaaa ccagggtgtc gggcttcaga aaagagccgt
     2461 gctcgatcga gtcgacgccg gcgttgatcg cctcgcgcgc ggcttggtcg ccgtgacaat
     2521 ggaccgcgac ttttttccgc aagcggtgcg attcgtccac cagcgccgcc atttccgccg
     2581 gatccaactg cggcacatcg acctcgtcgc ctaacgaaag gacgccgccc gagacagcgg
     2641 ccttgatcac gtcggcgcca ttcttcactt cgtaacggac ggctttccga atcgcgtccg
     2701 gcccatccgc aataccgttg gaccaatccg cctcggcgcc gaaaagcagg tcgcgaaatc
     2761 cgtttgtatc gtcgaaatgg ccgccggtgg cgccgatccc gtgggtggcg acgagcatcc
     2821 gcggtccggg gataaggcct tcgttaatta cgttgcggag cgagacatcg gggaagtcgt
     2881 gcgagtttgg aatccgactc ccgaggtcgc gcacactggt aaagccggct tcgagggtgg
     2941 cgcgcgcatg cgggatcgtt tcgagtgcga tctgggaaac attctcgtcc agcgagcgga
     3001 ggcgtcgctc gttgtagttg ccggaatagt cggccgtcag atgggtgtgc gcgtccatga
     3061 atcccgggga aagcgtcgcg tcaccgaggt cgatgatctg cgcgtcgccc gggatgggaa
     3121 gattgctgcc tacggcggta atggtgttgc ccgtgacgat gacgacgccc ttagggagca
     3181 gggtcttcgt cttgccatcg aaaagacgcg cggccttaag cgcgatcgtg accggcggca
     3241 agtcggctgc gagagcgctc gttaggccgg agagcgcgca gattgcgcgg agcagaaaga
     3301 aaacacgttt catccggccg gtataggaag ccggcgggca gattgcaact gtagacgctt
     3361 cgcacagcga agcggctaca gcgccggcac cactaactcg gtttcataaa ttgaaccgtc
     3421 cggcaggagc agactggatt tgagttgaaa ggaatcgacc cgcacgaggc cggcgtcaaa
     3481 atcggcctgc gcgcggagaa agggacgaat cgactcggcc gaaacatcct ggcaacgggc
     3541 cagcgtgagg tgcggatgcc agggacgaag atcggcttcg agacccgcgc cgagcaccgc
     3601 gtcggtgact cttttgtgca gttgaaagag atgcggatgc ccggcgccga cgccgatcca
     3661 aatgattttc ggccggcctt tccccggaaa aacgcccagg ccacaaactg gaagaaaaaa
     3721 agcggtaaag cggatggcgc tcaactccgg gcgcagtttt tcctccgcgt cggcggccac
     3781 atcgcccaga aaaccgagcg tcagatggat ttgctccgcg ctcagccagc gaattccgcg
     3841 ccggtgaggg ttcagcctaa cgagcagctc cgcgaccgat tccggcagtt caagactaac
     3901 gaaaagacgt ttcgctcgca ccgccgcggc tcacgtttcg tggcggcgtc cgatcgccac
     3961 gtcgcccaga cgactgagcc agcccggcac ccgctgcgcc acgcgtgcct ggcggccgga
     4021 tttcaattcg tccccttcaa ccccgaccgg accgggaagc atccggttga cgagtttcat
     4081 aaattccccg gtgaggctcg ggaaaatggc attgccgatg atcgcgacac gcgccggcca
     4141 accgatgatg agcgaggatt gcccgcggcg acaggcggcc acaatttttt ccgcggcccg
     4201 ctcggcattg accgcggcga agggcagcgc ggtcgagagc gagaaccagg taaactccgc
     4261 gccgtgatcg cctttgaagg tggcattcgc ctgcgaaccg gtccgcatca ttccgggcgt
     4321 gacggtggtg aggtggatgc cttctagcgc gagctcggcg cgaaaggcgt cggagagccc
     4381 gacgagcgca aacttgcttg cgctgtaggg cgcgagatgc ggcacggcca ctttgccgcc
     4441 gatcgacgcg atgttgacga tccgaccgcc accggcccgg cgcatatgcg ggatgacctg
     4501 ccggatcaaa ttgaaaggcg cccaaaaatg gagctgcatc gatttttcga aatcctgacg
     4561 ccgcatgttc tcgagcgggc ccacttcgat gatgccggcg ttgttaatca aaacgtcaat
     4621 cccgccgaaa cggtcgacca cggtctcaat tgcgcctaac gtctgcccac ggtcgagcag
     4681 atcacagggc acggccagcg cttcgcctcc gagcgcgcgc agctcagcgc aggcgcgcgc
     4741 cagctcgtct gcatcgcgcg cgaggagcgc gacccgtcca ccttccgccg cgatccggcg
     4801 ggcgatcgtc cggcccaacc cgcgggagcc gccggtgatt aagatggatt ttccggcgaa
     4861 agaaaaatcg ttgcgcagca ggcgcgagac ggcccaggtc gcgcaaccga ttccaccgac
     4921 ggtgagcaac aacgagcgct tcatccccgg actgtaacag cgaaaaccag agcaggcgac
     4981 cgacgcgggc tcggctgggg atttaaacgt cggcttcgac tggcgtggct tccggagtgc
     5041 ggtcagcgct cggtttgcgc acgctgaagg caagggtaaa cgccaccagc aggatgagcg
     5101 cgctggcgaa aaacggcgtg cgcgcataac gagtgagcgg ccggccgaga tcaaacatga
     5161 gcagccagcc gccgatcagc gggccaatca gccgtccgct actgccggcc gattgcatga
     5221 gtccgagcgc gcgtccctgc cagctacgat cgatcatctg cgaagccagt ccgttgagcg
     5281 tggggttgac cagtccgttg gcaatcgcga gcgcggcgca ggcgaggagc aacagcggca
     5341 ccgtggacac gaacgggagg aacgccatcg cgacggccag caggagcaaa ccggtgcgcg
     5401 ccagggcggt ttcgccaaag aacctgatca gccgaccgat caaaccgccc tgcagaatga
     5461 cgctgatgat tccgatgaaa ccaaacacgt aaccggtctg gcgcgcatcg aagccgaaat
     5521 gtttctccgt aaaaagggcg aagagcgtcg tcatgatggc gaagccggtg atgtggaaaa
     5581 aataggtggc cacgatcgtg ccaaacatgc gggcgtgccc gtgccgaaaa acctccgcca
     5641 ggccggagcg cttgtgcggt tgcgagcgat attccgggga aagactttcc ggcaggatga
     5701 agtagagcag gaccacgttc acggcggcga gcgcggcggc gaagtaaaag ggcgcgccat
     5761 aggaaatgtg gctcatgatt ccgccgatca tcgggccaaa ggtgaaaccg aggccgaagg
     5821 cggcgccgat cacgcccatc gagcgcgagc ggttttccgg cgtgctgatg tcggcgatat
     5881 aggcctgcgc ggtggagatg ttgccgccgc tcgcgccatc gataatgcgc gcgacaaaga
     5941 ggagcgggag cgccgaggcc caacccatga tcaggaagcc gagcgcggtg ccggccagac
     6001 taacgatcag caccggccgc cgcccgaagc gatcggagag ccggccgagg atcgggacaa
     6061 aaacgatctg catcccggaa tagatgccgg tgagccagcc aatctgcact ggagtggcat
     6121 gaaaatgctc cgcgtagagc ggcagcaccg ggatgatgat gccgaacccc accatgtcga
     6181 tgaagacggt gaggaagatg agaaaaagcg gagacatcgg aaaggatgaa ggatcaatgc
     6241 ggaaggatga aaagtgaaat cctaacgagg cattagaatg acgaattccg aatgccgaag
     6301 gtcgaatcaa ttcccaaact cgaatgacga accaaaatcg gctggcggcc gatcatgcga
     6361 ccttcgtcct tcgccattcg gaattcggaa ttcgccattg acccctcacc cgtgtttccg
     6421 attgaagcgc gccacttccc gcgccgtctc ccgatcggtt gcacgtttct tcagatcgag
     6481 ccgcttgtcc ctcgcttcct tgccctgcgc cacgccgatc tcggctttga tccgcccgtt
     6541 tttccaatag agcttgagga tgacgagcgt ccggcccgcg atattcgtta ggccgtaaag
     6601 tttgtcgatt tcgcgccgat gcaggagcag cttgcggacg cgtttcgggg ccggcacttc
     6661 gtggctcgcg cgctcatacg gctggatgtc ggcgccatag agaaaggcct cgtctttttc
     6721 gatgcgggcg aaggcgtcgc cgatattcgc cttgccggcg cggatggact tgacttcgct
     6781 gcccttgagc tcgacccccg cctcgaagcg ctcgaggatg tggaaatcgc gcagcgcctt
     6841 gcgattgaga atggtctcag cgggcatcgg aaagtgataa gtgacgagtg atcagcggga
     6901 aacgcggcgc atcccagtcc ttactcgtca cccacaattt atccgccgcg gcggactcgt
     6961 cactgcccta cgccccggcg ggcgtgtcct tcagctcctc gtaggtgagc ttgccggcaa
     7021 taatttcctt caaggcgatg tcggtgaggg aggacttggg ggtcgcttca accagcggac
     7081 gatgcccgat gccgagctgt cggacgcgct tggataccac gttgatcagg agttgctggt
     7141 tcgggatcac tttggctgct tctagcagga gttgggcggt catatttctc aaggatcgga
     7201 cggaacggcc gggttccgac ggaaattgca ggatcgtcgg cgacccgttc gttagggctg
     7261 aagaggtgga aatcgaagca agcatcgtcg ccgcaagcgg actcggataa tgcgtgcagc
     7321 cggagggttg tcaacggatt tctcgtcccg attgaatcac ttgccaggct cggcgaattg
     7381 cgcgaagtag gggcgctgcg tcgcccccgg cccaatggtc ggcgggggaa tgcgcggcaa
     7441 gaaattcggg ctgtggctca gcttggtaga gcgcctgcct cgggtgcagg aggtccaggg
     7501 ttcaaatccc tgcagcccga tcttatcccg cttttttgga cggcgacgaa ttgaagcaca
     7561 ccatcccgct gccattgctt cgatgaccga ctccgttctc accacgatcg ccgtcaccgg
     7621 ctttacgatt gcgttttttc acgcggccat tccgacccat tggctccctt tcgtgctcac
     7681 cgcccgcgcc caaaaatgga gccggccgaa gacgctcgcc gtgacggccg tcgcggccag
     7741 cgggcacgcc acttttaccg cctcgctcgg cctcattgtc gcctggctcg gcatcgcgct
     7801 gagcgatcgc gtcggaatct ggttcccgcg gatcgccggc ggggcgctca ttcttttcgg
     7861 gctagtctac gtcatccggc aattaaccgg gcatacgcac acccacctcc atctggggca
     7921 ggcccactcg catgatcatc atcacgaggg gcacgaccat ggcggaaaac ggctgtcgct
     7981 ttcgaagtcc gatcttgcag ccatcaccag cctgctcgcc ttgctcacct tttcgccctg
     8041 tgaagcgttc gtcccgatct acgtttcagg tgtgcgctat ggctggccgg ggttcgcgct
     8101 ccttaccatc atcctcaccg tcgccacgat cgccgggatg gtcgctttca ccgccctcgc
     8161 cctcaccg
//
LOCUS       DATMNS010000086         3240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_473175, whole genome shotgun sequence.
ACCESSION   DATMNS010000086 DATMNS010000000
VERSION     DATMNS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473175"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..>3240)
                     /locus_tag="VNW28_03220"
     CDS             complement(156..>3240)
                     /locus_tag="VNW28_03220"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08965.1"
                     /translation="LAAHREPSGKLTIFVGSASGGVWKSEDSGTRYRPVFDEQPVQSI
                     GAIALDPKNAKNVWVGTGEPWVRNSVSIGDGIYKSTDGGETWNKSGLPNSERISQIIV
                     SPKSSDTVYAAVPGALWSDSPDRGLYQTTDGGKTWNLILKGSNLSTGASTLAMDPTNP
                     NILFAGLWDFRRKGWIYRSGGETPNDPSASGLFRTTDGGATWKEITPEANKGFPKKPY
                     GRLAVAIAPSNPKRVYCFVESTNSALFVSDDGGQTWNQRDKSQWMVWRPFYFANLIVD
                     PKNPNRVFKTDGALILSEDGGKSFATVGGFNGAHGDVHVVWVDPTNPQTVISGDDGGI
                     WYAYDGGNKWWKANNLPISQFYHVNVDDADPYQVYGGLQDNSCWVGQSEYPGGITNAQ
                     WENLFNGDGMFTFVDPADPDYIYAEYQGGHVGRVNRHTLEGRIIQPKANYKEKLRFNW
                     NTPLVASPNEKGTIYIGAQFLFRSRDHGQSWERISPDLTTNDPEKQKQEQSGGVTVDN
                     SEAEMHTTIYSISESPKDKNLIWVGTDDGNVQLTRDGAKTWSNVVGNVPGLPKNSWVN
                     WVEASKFDPATAYAAFDRHTFGDFAPYVYKTTDYGQTWKSLVTPQDAKGVRGYAYVIK
                     EDLVQPNLLFLGTEFGLFVSIDGGEAWAQYKGSRFPAVAVRDLAIQPRTNDLVLATHG
                     RGIWIIGDITPLRSLTPELLTQEASFVAANPVQQQRIESNGGWPNGAAVFTGDNPPDA
                     AVITYYQKSRHLFGKLKIEILDAAGKVIDSLPASKRPGLNRVTWSMHEKPPRVPPAAQ
                     IAFAGVTGPRVLPGTYTVRMTKNDKPYEMKLNVGLDRRDKFSDADRKAQYDAAERVKA
                     LFGDESAFMDRIMALRAGLAKSGAALKQDDALRQAMTEFGTKVDAVRKQIVATTEGGA
                     ITGEERIREHTDQLYGAILSYEGKPADYQIARIDSLRRELDDAMKDFDGLLTNELPKL
                     NETLKAKGQPEISPPPAKVAVYESESQTGGGPTGSVDRDLPASAVSLPTNFRLLR"
BASE COUNT          550 a          961 c         1015 g          714 t
ORIGIN      
        1 cgcgcgtctc gcgtactggt ttctgcgtcc cgcagaaaca ggctttgctc acgttcagca
       61 taggaagttc gcgacggtga gacaccgtcg ccagcacgcg ggacgcgtgc gctaccctgc
      121 ctcgtggctg cgacgcgctg acgaattagg tccgcttagc gtagcagccg gaagtttgtc
      181 ggaagcgaca ccgcactggc tggcaaatcg cgatcaacgg aaccggtggg tccaccgccg
      241 gtctggctct cggactcata caccgcgacc ttcgcgggcg gtggtgaaat ttccggttgg
      301 cccttcgcct tcaacgtctc gttcaatttc ggcagttcgt tggtcagcaa gccgtcgaaa
      361 tccttcatcg cgtcatccaa ctcgcgccgg agcgaatcga tccgcgcgat ctgataatcg
      421 gccggcttgc cctcgtagct cagaatggcg ccgtaaagct gatcagtgtg ttcgcgaatg
      481 cgttcttcgc cggtgatcgc gccgccttcg gtcgtggcca caatttgttt gcggacggca
      541 tcgaccttgg tgccgaattc ggtcatcgcc tggcgcaggg catcatcctg cttgagggcc
      601 gctccgcttt tcgcgagtcc ggcgcgcaag gccatgatcc gatccatgaa cgcgctctcg
      661 tcgccgaaca aggccttgac ccgctcggcc gcgtcgtact gcgctttgcg atccgcgtcg
      721 ctgaacttgt cgcgtcgatc gagccccaca ttcagcttca tttcatacgg cttatcgttc
      781 ttcgtcatgc gcaccgtgta ggtgccgggc agaacgcgtg gaccggtcac gccggcgaag
      841 gcaatctgcg cggcgggcgg cacgcgcggc ggcttctcat gcatgctcca ggtgacccgg
      901 ttgaggccgg gacgtttgct ggccggcagg ctgtcgatca ctttgcccgc cgcatcaagg
      961 atttcgattt tcaacttacc gaaaaggtga cgcgattttt gataataggt gatgactgcg
     1021 gcgtccggtg gattgtcgcc ggtgaatacc gccgcgccgt taggccaacc gccattgctc
     1081 tcgatgcgct gctgctggac cgggttcgcc gcgacgaagc tggcttcctg cgtgaggagc
     1141 tccggggtga gcgagcgcag cggggtgatg tctccgataa tccagatacc acgcccatgg
     1201 gtggcgagca cgagatcgtt tgtccgcggt tgaatggcga gatcgcgcac cgcgacggcc
     1261 gggaaacggc tgcccttata ttgggcccac gcctcgccgc cgtcgatcga gacaaagagt
     1321 ccgaactccg tgccaaggaa aagcaggttt ggctggacca ggtcctcttt gatcacgtac
     1381 gcatagccgc gaacgccttt cgcatcctgc ggagtgacta acgacttcca ggtctggcca
     1441 tagtcagtcg ttttgtaaac gtagggcgcg aagtcaccaa aggtatggcg atcgaacgcg
     1501 gcgtaggcgg tggccggatc gaacttgctc gcctccaccc agttgaccca cgaattcttc
     1561 ggcaggccgg ggacgttgcc gacgacgttg ctccaggtct tggctccgtc gcgtgtgagc
     1621 tggacgttgc cgtcatcggt cccgacccag ataaggtttt tgtccttggg cgattcgctg
     1681 atggaataga tggtggtatg catttccgcc tcggaattgt ccaccgtcac gccaccggat
     1741 tgctcctgtt tctgtttttc gggatcgttc gtcgtcaggt cgggcgaaat gcgttcccag
     1801 ctctggccgt ggtcgcggga gcggaaaagg aattgcgcgc cgatgtagat ggtccctttc
     1861 tcgttaggcg aagcgaccag cggcgtgttc cagttgaagc gcagcttttc cttgtaattc
     1921 gccttcggtt ggataatgcg gccctcgagg gtgtgccgat tgacccgtcc gacgtggccg
     1981 ccctggtatt cggcgtagat gtaatccgga tccgccgggt cgacgaaggt aaacatgcca
     2041 tcgccgttga agaggttttc ccactgcgca ttggtgatgc cgccggggta ttcagactgg
     2101 ccgacccagc aactgttatc ctgcaggccg ccgtagacct gatacgggtc ggcgtcgtcc
     2161 acgtttacgt gataaaactg cgagatggga aggttgtttg ctttccacca tttgttgccg
     2221 ccatcgtagg cgtaccaaat ccccccgtcg tcgcccgaga tgacggtttg cgggttggtc
     2281 gggtcgaccc agacgacgtg cacgtcgccg tgcgcgccgt tgaagccgcc gacggtggcg
     2341 aaactcttgc cgccgtcttc gctcagaatg agcgcgccgt cggtcttgaa gacgcgattc
     2401 gggtttttcg gatcaacgat caggttggca aaataaaacg gccgccagac catccattgg
     2461 ctcttgtcgc gctggttcca cgtttgcccg ccgtcgtcgg agacgaagag ggcgctattg
     2521 gttgactcga cgaagcagta gacgcgcttc gggttcgagg gcgcgattgc gaccgcgagc
     2581 cggccgtagg gtttctttgg aaaacccttg ttcgcttccg gggtgatctc tttccaggtg
     2641 gcgccgccgt ccgtcgtgcg aaagagtccg ctggccgagg gatcgttagg cgtttcgccg
     2701 cccgagcggt agatccagcc tttgcgccgg aaatcccaga gcccggcgaa aagaatgttc
     2761 gggttcgtcg gatccatcgc gagcgttgag gcgccggtgg aaaggttgga acctttgagg
     2821 atcagattcc acgtcttccc gccgtcggtc gtttggtaga ggccgcgatc gggcgaatcg
     2881 ctccagagcg cgcccggcac cgcagcataa accgtgtcgc tgctctttgg actaacgatg
     2941 atctgcgaaa tgcgctccga gttgggcagg cccgatttgt tccaggtctc gccgccgtcg
     3001 gtcgatttgt agatgccgtc gccgattgag acactgttgc ggacccatgg ttcgcccgtg
     3061 ccgacccaga cgttcttcgc gtttttcggg tcgagcgcga tcgcgccgat cgattgcacc
     3121 ggttgctcat cgaagaccgg gcgataacgc gtgccgctat cctccgattt ccaaacgccg
     3181 ccactggccg atccgacgaa aatcgtcaac ttgccggaag gttcgcggtg ggcggcgagg
//
LOCUS       DATMNS010000087         2663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_834222, whole genome shotgun sequence.
ACCESSION   DATMNS010000087 DATMNS010000000
VERSION     DATMNS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834222"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..313)
                     /locus_tag="VNW28_03225"
     CDS             complement(143..313)
                     /locus_tag="VNW28_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08966.1"
                     /translation="MKFTILLAAGAALLLGACASLDKTQETVTEIPAHDSTTHAQVPA
                     QDSSKIPGNQNF"
     gene            complement(363..1595)
                     /locus_tag="VNW28_03230"
     CDS             complement(363..1595)
                     /locus_tag="VNW28_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXA08967.1"
                     /translation="MTPPLYIAFRFLGHRKRAFFLSLGGVVFGVAIFICTQAQTQGFA
                     KNFIDSTLGSNGALVMRSRIQTLEGAAELPPLPVHPPHRRLFEGISNASEIMRVSRQF
                     SNVVACSPVLRGTLTAQAGFQNATVDLFGIDLPLHLQTTDLARQIETGSLDDFRNNPN
                     SLIVGSRLAAALQVEVGESIQLLSPDGEYRRFVVAATARSGVGVVDSGRIYCHAKVAQ
                     SLLKKPYTASLIIYKLRDPGRAPALAAHFEKLFNHETESWQEREEANLQLFLTIRLSA
                     AITVSLIILLAGFGIFNVLTMTVLSKVKEIAILRSIGYRRGDISAIFLWQGAMIAGAG
                     SLIGCGAGALLTLLVGHIPIRVRGLIYANHFLVTWDWRHYLWATLLAIIAVAIASYVP
                     AHRASLLSPVATLRGTSG"
     gene            complement(1592..>2663)
                     /locus_tag="VNW28_03235"
     CDS             complement(1592..>2663)
                     /locus_tag="VNW28_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXA08968.1"
                     /translation="PVAEVERVQRGTAISAVYGTVRIEPSFVNNVRAQNAGFIHLAPP
                     LAAGRGAIGRPVTKGELLAAIADESTARQLKQAQADLQAAVESAQLELPSAEPLKIAE
                     GNVERQEKLAALSNVPAVEYEKAKSEVNRLRAALKTETIERDRNLEALRTGVQKIEAQ
                     MKNSEIRAPMDGILSGLRIIDGELVVEGNELLTVSSRTNYVRGEVDEEDVGEVKAGMK
                     ADLQMYAYRTRQFSATVSSVLPAADPETQRYTVILDLEKPPPNLMAGMTGEMNIITGR
                     HENALLVPTRALLVDQVLVVAFGMVESRTVKVGYRTLELSEALEGLKEGALVVVADQD
                     KLHPGQFVRRRLVGPDQPATRQ"
BASE COUNT          491 a          775 c          893 g          504 t
ORIGIN      
        1 agctccgcgc cagcaaaggc taaaggcgga agtggagttg gagatcaggg aacgggggga
       61 gtgcctgcgg tcacgccgaa ggactggaga ggctgcggtc gcggaagcct ggggtgcggg
      121 ggtcgatgac cgccattcac agctagaaat tctggttgcc cggaattttg gacgagtctt
      181 gcgcggggac ctgggcgtgc gtggtgctgt cgtgcgcggg gatctcggtg acggtctcct
      241 gagttttatc aaggctcgcg caagccccca gcaagagggc cgcgccggcg gctaacagaa
      301 tcgtgaactt catcctccaa tgatgcttct ccgattagcg atcgacaacg ggaatcgaca
      361 ggttatccgc ttgtcccgcg gagggtggcg acgggcgaga gcaagctggc gcggtgggcc
      421 ggcacgtagc tcgcgatcgc gaccgcgatg atggcgagca gcgtcgccca gagataatga
      481 cgccaatccc aggtaaccag gaaatggttc gcatagataa ggccgcgcac ccgaatcggg
      541 atgtggccca cgagcagggt caggagcgcg cccgcgccgc agccgatgag cgaaccggcg
      601 cccgcgatca tcgcgccttg ccagaggaag atcgcgctga tgtcgccgcg gcgatagccg
      661 attgagcgca ggatggcgat ctcttttacc ttcgagagca cggtcatggt gaggacgtta
      721 aagatgccga agcctgcgag caggatgatg agcgagacgg tgatggcggc cgaaagtcgg
      781 atcgttagga acaattgcag attcgcttct tcgcgttcct gccagctctc ggtttcatga
      841 ttgaaaagct tctcaaaatg ggccgcgagc gcgggcgcgc ggcccgggtc gcggagttta
      901 taaatgatga gtgaggccgt gtaaggtttc ttgaggagac tctgcgcgac tttggcgtgg
      961 caatagatcc ggccggaatc gaccacgccc acaccgctgc gcgccgtcgc ggcgacgaca
     1021 aaacgccggt attcgccgtc gggcgaaaga agctgaatgg attcgcctac ttccacctgg
     1081 agcgccgcgg cgaggcgcga accgacgatg agcgagttgg ggttgttgcg aaagtcgtcg
     1141 agcgatccgg tttcgatttg ccgggcgaga tcggttgtct gcagatggag gggaagatcg
     1201 atcccgaaaa gatcgacggt cgcgttttgg aatccggcct gcgcggtgag cgtgccgcgg
     1261 agcaccggcg agcaggcgac gacattcgag aattgccggc tcacccgcat gatttccgat
     1321 gcgttgctga tcccttcgaa gagacggcga tgcggcggat gcacgggcag gggaggcaac
     1381 tccgcggcgc cctcgagggt ctggatgcgc gaccgcatca cgagcgcccc attacttccg
     1441 agggtggagt cgatgaaatt tttcgcgaaa ccctgggtct gggcctgggt gcagatgaag
     1501 atcgcgacgc cgaaaaccac gccgccgagg ctgagaaaaa aggcgcgttt ccggtggccg
     1561 agaaaacgaa acgcgatgta gagcggcggc gtcactgtcg ggtagccggc tgatccgggc
     1621 cgaccaggcg tcgcctaacg aattgtccgg gatgcaattt gtcctggtcg gcgaccacga
     1681 cgagcgcgcc ttcctttagg ccctcgagcg cctcgctcaa ctccagcgtg cggtagccga
     1741 ccttgacggt ccgtgattcc accatgccaa acgcaacgac caggacctgg tcgacgagca
     1801 aggcccgggt cggcacgagg agcgcatttt cgtggcggcc agtgatgatg ttcatttcgc
     1861 cagtcatccc ggccatcagg ttcgggggcg gtttttccag gtcgaggatc acggtgtaac
     1921 gctgggtctc gggatcggcc gcgggcagga cggatgaaac cgtggcgctg aactggcggg
     1981 tgcgatacgc atacatctgc aggtcggctt tcatcccggc cttcacttcg ccgacgtctt
     2041 cctcgtccac ctcgccacgc acgtaattcg tccgcgatga aaccgtgagg agctcattac
     2101 cctccacaac gagctcgcca tcgatgatcc ggagcccgga aaggatgcca tccatcgggg
     2161 cgcggatttc gctgtttttc atctgcgctt cgattttttg cacgccggtc cgcagcgcct
     2221 cgaggttgcg gtcgcgctcg atcgtttctg tcttgagcgc tgcgcgcagg cggttcactt
     2281 cgcttttcgc tttctcgtat tcaaccgccg ggacgttgct cagcgcggcc agtttttcct
     2341 gacgttccac atttccttcc gcgatcttga gcggctccgc ggagggcaat tccagttgcg
     2401 cactttcgac tgccgcctgc aaatcggcct gcgcctgctt gagctgacgc gcggtgcttt
     2461 cgtccgcgat cgccgcgagc aattcaccct tggtcactgg ccggccgatc gcgccgcgcc
     2521 cggcggcgag gggtggggcg agatgaatga agccggcgtt ctgcgcccgg acgttattca
     2581 caaacgacgg ctcgatccgg accgtgccgt aaaccgcgga gatggcggtg ccgcgctgga
     2641 cccgttccac ttcggcgacc ggc
//
LOCUS       DATMNS010000088         9495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_122837, whole genome shotgun sequence.
ACCESSION   DATMNS010000088 DATMNS010000000
VERSION     DATMNS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9495
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122837"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VNW28_03240"
     CDS             <1..1167
                     /locus_tag="VNW28_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXA08969.1"
                     /translation="KAVELIRQDYVDGSKVSYHDLIYAAMKGMLSSLDPHSQFMDPDD
                     FKDMQDDTRSRFNGLGVEVAVRDGVLTVVTPMEDTPAAKAGIQAGDQILKINGASTER
                     LGLQDAISVLRGEPGQKITLTILRPSTREIKDYPLERKEIKVPSVKGARLLEPELTGP
                     FKVGYVRIVQFNEPTAEEFGQALDNLQKQGMQALVLDLRNNPGGLLNSAVDVLGQFLP
                     PNTKVVSTQGRATSQKHDYSTPGGVKERPRFPLVVLVNEGSASGSEIVAGALKDLHRA
                     IVVGETTFGKGSVQNVMQMPDGSALRFTTAKYYTPSKTVIQGNGVAPNILVPLTPEQD
                     RAVAVLRNNDKIQPAEAIGIIRANDLQMMRGIDALKGVMIYAEQTPPKVDAVKK"
     gene            1256..2251
                     /gene="tsaD"
                     /locus_tag="VNW28_03245"
     CDS             1256..2251
                     /gene="tsaD"
                     /locus_tag="VNW28_03245"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HXA08970.1"
                     /translation="MNPLLLAIETSCDETAVAVLRGRVLLASEVASQGAAHQPYGGVV
                     PEVAARHHLRHAPRLLARATRDADISLGQVAAFAATSGPGLASSLMIGASLAKGLALG
                     FDKPYLAINHLEGHLLSPFFGSEAGIEPNVALIVSGGHTMLVEVSGVADYRVIGRTVD
                     DAAGEAFDKVAKMLGFAYPGGPEIERCAQGGDPARFDLPRSMLHSGDANFSFSGLKTA
                     VRYLLPKISPDDQRDLCASFQRAVVDVLVAKTIAAARAARCRLVTVSGGVSCNGELRA
                     RMSLGCEQAGLELRFAAPLLCTDNAAMIAFAALLRLERGASTPLTTEIDPNLALV"
     gene            complement(2399..3280)
                     /locus_tag="VNW28_03250"
     CDS             complement(2399..3280)
                     /locus_tag="VNW28_03250"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HXA08971.1"
                     /translation="MDLKRYLAARQKEVDRTLDRFLPRASTKPATIHKAMRYSLFAGG
                     KRLRPILCLAAAEACGGLTNTALPLACAMECIHTYSLIHDDLPSMDNDDFRRGRATCH
                     KVFGDGIAVLAGDALLTIAFEIAATTEAWPRYDLRAIFREISIAAGSRKLIAGQVADL
                     EGEGQRINHAQLRYIHENKTAALLTTSVRLGAMSANATPAQLKAVSDFGRALGLAFQV
                     IDDILDVTQTSEKLGKSAGKDVAAKKATYPAVMGLEKSRAEAGRLTRQAHSALHPLGK
                     RAETLRALANYLLVREY"
     gene            complement(3356..4162)
                     /locus_tag="VNW28_03255"
     CDS             complement(3356..4162)
                     /locus_tag="VNW28_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HXA08972.1"
                     /translation="MHPRKYLTVFNLGIQNTFVYRWNYLLRAIFGVIPLVGTIFLWRA
                     IFHESGGGMRGYDYGSMIYYYLLTILVSNLVTPTEDEWQIAADIREGQINSFLTKPVN
                     YLGYRFSIFLSGRLVYTAVTILPIAAIFFYFRRFIILPNDVLTWLAAFVSLLLSALIQ
                     FFLTYALSMMAFWILEISTIVFIVYSFEYFLGGQMFPLDIMPPAIQAVMKWLPFYYEL
                     FCPIAIFQGRLRGAEMWSGLAIQSGWMLSMWAAANLMWKRGLGHYQAVGG"
     gene            complement(4249..4872)
                     /locus_tag="VNW28_03260"
     CDS             complement(4249..4872)
                     /locus_tag="VNW28_03260"
                     /inference="COORDINATES: protein motif:HMM:NF021511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HXA08973.1"
                     /translation="MNTRISPLHLVLLGAIGLVFAWSGWHPYDRLTWWLEVAPGILGL
                     VLLIATYSRFRFTTLVYTLIALHMMLLGVGGHYTYARVPLFTWIEPIFGWQRNHFDRL
                     GHFAQGFVPALIARELFIRLRVVSSSGWRFAIVVLICMGISALYELLEWVTALTAGSA
                     STAFLGTQGDVWDTQEDMFMALIGAICALVLMSRWQNKQMRKLPSRL"
     gene            complement(4869..5753)
                     /gene="pdxA"
                     /locus_tag="VNW28_03265"
     CDS             complement(4869..5753)
                     /gene="pdxA"
                     /locus_tag="VNW28_03265"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HXA08974.1"
                     /translation="MHPRIGITLGDPAGIGPEIVRGALASGRLPEADYRLLGATAGHS
                     LGKPNPAGARAALSALEEAATLALAGEIDAVVTGPIHKAAMYEVGFQFSGQTEFFAAR
                     CGVQNFAMLLTGAGLTVALVTTHIPLASVPENLRTHEIVRVGSLLADFLAHRGGGCRV
                     AVAGLNPHAGESGALGREEIEIIAPAIAQLNRSSLVTQPSPPPFSGPFSPDTVFHRAA
                     EGEFDAVLCMYHDQGLIPLKLHAFHEGVNVTLGLPFPRTSPDHGTAFAIAGQGIARAD
                     SMTAAIRLAVELVTARAQ"
     gene            complement(5874..6869)
                     /locus_tag="VNW28_03270"
     CDS             complement(5874..6869)
                     /locus_tag="VNW28_03270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXA08975.1"
                     /translation="MVKATLNFIWRYPMATLLAFSCLALANTAPTLGAESVNGIAAVV
                     NDKVITYSEVRDVCEPRERLLRSQYSGDELVKKITDLRKAALQDLIDRQLIIQAFEKE
                     KFAIPSHFVDERVDAIIKDNFGGDRNAFIKTMEAQKYTMSKFRDLERDKIIIQAMRSK
                     NVKSNLLVPPAKIDEYYRQHRDQFSSKAQVKLRMIMIPGHLDSGGGAAQKSMAEEIRA
                     KLITGADFDKMAQMYSEDSTRDLGGDWGWIDDKTLSAPLSEVAFSLKPGEISKVVDIG
                     GNHYILKVEARQGGEAKPLKDVRADIEGRLRQEQAQQLQEHWLASLRAKAYIKTF"
     gene            complement(6851..>9495)
                     /gene="mfd"
                     /locus_tag="VNW28_03275"
     CDS             complement(6851..>9495)
                     /gene="mfd"
                     /locus_tag="VNW28_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HXA08976.1"
                     /translation="FAVRGGILDVFSWQAQAPLRAEFFGDEVESLREFDLDTQVSLRD
                     LDQAELLLGAADDQSGRVRDYIGTGHLTVAIEEGHEAADIGISEGWIESQGEIEDFSG
                     AFVDCELGEFDTGEFIIAEAKRRQFFARLTTWQENGARIAIYFQTEGEIERFREIMAE
                     TTISLGGIDLIEGTLARGFCFPAAHLVVLAAAELFGRAPTHGRRRLQRTAKFGTHRAQ
                     IDFSELNEGDLVVHLEHGIGRFLGLQRFGGAEAGEVLAIEFAEEAKLYVPLEQAYLVS
                     RYVGVGKKSPALSSLADAKWTRAKKNAASSIFDYAGKMLALQAERETQQGHAFGHDTK
                     WQLEFEHSFPFRETPDQLKAIAETKRDMEEPRSMDRLICGDVGFGKTEVAIRAAFKAV
                     MEGKQVAVLTPTTVLAQQHFETFRQRMLDYPVRIEMLSRFRSPPEQRKVLEQLREGGV
                     DVVVGTHRLISGDVVFKDLGLVVIDEEQRFGVLHKEKFKELFKLVDVLTLSATPIPRT
                     LYLSLVGVKDMSTIETPPLNRLPVETVICGYDERIIRDAIQRELERQGQVYFLHNRVV
                     SIGKVRDRIAELCPGARVEIGHGQMDADELEAVMQRFVAGKIDVLVCTTIIESGLDIP
                     NANTIVIDRADRFGLADLYQLRGRVGRAEHKAYAYLLLPRELMTVGAARKRINAIQQY
                     SSLGAGFRIAMRDLEIRGAGSILGTAQSGHIVAVGFDLYCQLLKQAVAQLKGEKLRPR
                     LEVEVRLDFVGTNEGEYLAVGPARLIPAFIPTAYISDAALRIQAYRNLAEIATVEMLQ
                     RLRRDWRDRFGKWPPAVDNLLLLSEIKLAAARGQVTRVEVREDKLMLSRRGDFILVDG
                     KFPRLTSGRIDLNLPEVWQWLKQL"
BASE COUNT         1888 a         2861 c         2965 g         1781 t
ORIGIN      
        1 aaggccgtcg agctcatccg gcaggactac gtcgacggca gcaaggtcag ctatcacgac
       61 ctgatctacg ccgcgatgaa aggcatgctc tcttcgctcg atccgcacag ccagttcatg
      121 gatccggacg acttcaagga catgcaggac gatacccgca gccgtttcaa cggcctcggg
      181 gtcgaggtcg cggttcgtga cggagtgctc accgtcgtga ctccgatgga agatacgccg
      241 gcggccaagg ccggcattca ggcgggcgat cagattttga aaatcaacgg cgcctcgacc
      301 gagcgccttg gactgcaaga cgcaatctcc gttctacgcg gcgagccggg gcaaaagatc
      361 accctcacga ttttgcggcc ctcgacccgg gagatcaaag attacccgct cgagcgcaaa
      421 gaaatcaaag tgccgagcgt gaagggtgcg cggctgctcg aaccggagct gaccgggccg
      481 ttcaaggtgg gctacgtgcg catcgtgcaa ttcaacgagc cgacggcgga agagttcggc
      541 caagccctcg acaatttgca gaagcagggg atgcaggcgc tcgtgctcga cttgcgcaac
      601 aaccccggcg gtttgctcaa cagcgcggtc gacgtcctcg ggcagtttct gccaccgaat
      661 accaaagtcg tctccaccca gggccgcgcg acgtcgcaaa agcacgatta ctcgacgccg
      721 ggcggggtga aggagcggcc gcgctttccg ctcgtggtgc tggtaaatga aggaagcgcc
      781 agcggctccg agatcgtggc cggcgcgctc aaagatttgc atcgcgcgat cgtggtgggc
      841 gaaacgactt tcggcaaagg ctcggtgcaa aacgtgatgc agatgcccga cggttcggcg
      901 cttcgcttta cgaccgcgaa gtattacacc ccgagcaaga cggtgattca gggcaatggc
      961 gtggcgccca atatcctcgt gccgctgacg ccggagcagg accgcgcggt ggccgtcctg
     1021 cgcaacaacg acaagatcca gcccgccgag gcgattggca tcatcagggc gaatgacctg
     1081 caaatgatgc gcgggatcga cgcgctcaaa ggggtgatga tttacgcgga gcagaccccg
     1141 ccgaaagtcg acgcagtgaa gaagtaacgg cggcggaatt atcaccgcag agcgcgccga
     1201 gcggtgcgaa ttctcgaaga tatttcattg tttcctctgg aaactctgcg gttagatgaa
     1261 ccctttgctc ctcgccattg agacatcgtg cgacgagacg gccgtcgccg tcctgcgcgg
     1321 tcgcgtcttg ctggccagcg aggtcgcttc gcaaggcgcg gcccatcagc cctacggcgg
     1381 tgtggtcccc gaggtcgcgg cgcgccatca ccttcggcac gcaccgcggc tgctcgcgcg
     1441 ggcgacgcgt gacgccgaca tttcgttagg ccaggtcgcg gccttcgccg ccacgagcgg
     1501 accggggctg gccagttcgc tcatgatcgg cgcgtcgctc gccaaggggc tcgcgctcgg
     1561 tttcgataaa ccgtacctcg cgatcaacca tctcgagggc catttgctct cgcccttttt
     1621 cgggagcgag gccggaattg agccgaacgt tgcgctcatc gtgagcggcg ggcataccat
     1681 gctggtggag gtgagtgggg tggccgatta ccgggtcatt ggacgcaccg ttgatgacgc
     1741 cgcgggcgaa gcgttcgata aggtcgccaa gatgctcggg ttcgcctacc cgggcggacc
     1801 ggagatcgag cgctgcgcac aagggggcga cccggcgcgg tttgatctgc cgcgcagcat
     1861 gcttcactcc ggcgacgcga acttcagctt cagcgggttg aaaacggccg tccgctatct
     1921 cttgccgaag atctctccgg acgatcagcg cgatctctgc gcgtcgttcc aacgcgcggt
     1981 agtcgatgtg cttgtcgcga agaccatcgc ggcggcacgc gccgcgcgct gccggctggt
     2041 gacggtgagc gggggcgtga gctgcaacgg cgagctgcgg gcgcggatgt cgttaggctg
     2101 tgagcaggcc gggttggagc tgcgcttcgc ggccccgctt ctctgcaccg acaatgccgc
     2161 catgatcgcc ttcgccgcgc tgctccgcct ggagcgcggc gcttcgactc ccctcacgac
     2221 cgagatcgat cccaacctcg cgctggtgta actccttttc cggggagcgc acgcgacccg
     2281 cgtgctggtt tcggcgactc gccgaaacag gctttcttct tcgggcggca cagagggaaa
     2341 agttcgcgat ggcggggtcg ccatcgccag cacgcgagtc gcgtgcgctc cccggaggtc
     2401 agtactcgcg cacgaggaga taattcgcga gcgcacgcaa cgtctccgcc cgtttgccta
     2461 acgggtggag cgcgctgtgc gcttggcgtg tcagccgccc agcctccgct ctcgatttct
     2521 ccagacccat gaccgccgga taagtggcct tctttgccgc gacatccttt ccggcgctct
     2581 tgccgagctt ctcgctcgtc tgggttacgt cgagaatgtc gtcgatcacc tggaaagcga
     2641 gcccgagcgc gcgaccaaaa tcactcaccg ccttgagttg ggccggcgtc gcgttcgcgc
     2701 tcatcgcgcc gaggcggacg gaggtggtca ataaggcggc cgtcttgttt tcgtggatgt
     2761 aacgcagttg cgcgtggttg attctctggc cctcgccctc gagatccgcg acctggccgg
     2821 cgatcagttt gcggctgccg gcggcaatcg agatctcgcg aaaaatcgcg cgcagatcat
     2881 aacgcggcca cgcttccgtg gtggcggcaa tttcgaacgc gatggtgagc aaggcatcgc
     2941 ccgccaggac ggcgatgcca tcaccaaaga ctttatggca ggtggcccgg ccccgccgga
     3001 agtcgtcgtt atccatgctc gggagatcgt cgtgaatcag cgagtaggtg tggatgcact
     3061 ccatcgcaca ggcgagcggg agcgcagtat tcgttaggcc accacaggct tcggccgcgg
     3121 cgagacagag gatcgggcgc aggcgtttgc ccccggcgaa aagcgagtag cgcatcgcct
     3181 tgtgaatcgt cgccggcttg gtcgatgccc tcgggagaaa gcgatcaagc gtgcgatcga
     3241 cctctttttg gcgtgccgcg agatagcgtt tcaaatccac gatcccgcga atgtggaaaa
     3301 tgccgggagg ttgcgcaacc ggaagttagg cgggacgcct aacttccggt tgcgctcagc
     3361 ctccgaccgc ctggtaatga ccgaggccgc gtttccacat cagattggcc gcggcccaca
     3421 tcgacagcat ccagccgctc tggatcgcga gcccgctcca catctctgcg ccgcgcaatc
     3481 gtccctggaa gatcgcgatc gggcaaaaca gctcgtaata gaagggcagc catttcatta
     3541 ccgcctggat cgcgggtggc atgatatcga gcgggaacat ctggccgccg aggaaatatt
     3601 cgaagctgta gacgatgaaa acgatggtcg aaatctcaag aatccagaag gccatcatgg
     3661 agagcgcata ggtcagaaaa aactgaatca gggccgacag gagcagggaa acgaaggccg
     3721 cgagccaggt taacacgtcg ttaggcaaaa taatgaaacg gcggaaatag aagaagatcg
     3781 ccgcgattgg gagaatcgtg accgcggtgt aaacgagccg gccgctgagg aaaatgctga
     3841 agcggtaacc cagataattc accggcttgg tcaggaagga attgatctgg ccctcgcgaa
     3901 tatcagccgc gatctgccat tcgtcctcgg tcggcgtgac gaggttgctc accaggatgg
     3961 tgagcaggta atagtaaatc atcgagccgt agtcgtagcc gcgcatgccg ccgccgctct
     4021 cgtggaaaat cgctcgccaa aggaaaatcg tgccgaccag ggggatgacg ccgaagatcg
     4081 cgcgtagcaa atagttccag cgatacacaa acgtgttctg gatcccgaga ttgaagacgg
     4141 tcaggtattt ccgcgggtgc acgaaagaga tttttaccac ggatgacacg gatggaagag
     4201 agattctgcc ggttctcctt ggattcgtgt catccgcggt ccgatatttc agagccggct
     4261 cggcagtttg cgcatctgct tattttgcca gcggctcatg agaacaagcg cacagatcgc
     4321 gccgatcaag gccatgaaca tgtcctcctg cgtgtcccag acatcgccct gcgtgccgag
     4381 aaaagcggtc gaggcggagc cggccgtgag cgcggtgacc cattccagaa gttcgtagag
     4441 cgcgctgatt cccatgcaga ttaggaccac gatcgcgaat cgccagccgc tcgatgacac
     4501 gacgcgcagc cggataaaca actcgcgcgc gatcagcgcc ggaacaaagc cctgcgcgaa
     4561 atgtccgagc cgatcgaaat gattccgctg ccagccgaag atcggttcga tccaggtaaa
     4621 tagcggcacg cgcgcgtaag tgtaatgacc gccgacgccg agcagcatca tgtgcagcgc
     4681 gatcagggtg taaacgagcg tcgtaaaacg gaagcgactg taggtcgcga tcaggagcac
     4741 aaggccgaga attcccggag cgacctcgag ccaccacgtc aggcgatcgt aaggatgcca
     4801 gccggaccag gcgaaaacga gcccgatcgc gccaagcagc acgagatgga gcggcgaaat
     4861 gcgagtattc actgggcgcg tgcggttacg agttccaccg cgagtcggat cgcggcggtc
     4921 atgctgtcgg cgcgcgcgat cccctgaccg gcgatggcaa aggcggtgcc gtgatcggga
     4981 ctggtgcgcg ggaaggggag accgagagtg acgttcactc cttcgtgaaa ggcgtgaagt
     5041 ttgagcggga tcaatccctg atcgtgatac atgcaaagga ccgcgtcgaa ttctccctcc
     5101 gccgcgcgat ggaagacggt gtcgggtgaa aacgggccgg agaaaggcgg cggcgagggt
     5161 tgagtgacga gtgacgagcg attgagttgc gcgatcgccg gagcaatgat ctcgatttct
     5221 tcgcgtccga gcgctccgga ttctccagcg tgcgggttga ggccggcaac ggcgactcga
     5281 cacccaccgc cccggtgtgc cagaaaatcg gcgagcaacg aaccgactcg gacgatctcg
     5341 tgagtccgca gattttccgg cacggaggcg agcgggatgt gcgtggtcac gagcgcgacg
     5401 gtgagccctg ctccggtgag cagcatggcg aagttttgga ctccgcagcg cgcggcgaag
     5461 aattcggtct ggccggaaaa ttggaatccg acttcataca ttgccgcctt gtgaatcggc
     5521 ccggtaacga cggcatcaat ctctcccgca agggcgagcg tggccgcttc ctcaagcgcg
     5581 gaaagggcag cgcgcgcgcc cgccgggtta ggtttgccta acgaatgccc ggccgtggca
     5641 ccgagcagac gataatccgc ctcgggtaac cggcccgaag cgagcgcgcc gcggacaatc
     5701 tccggcccga tgcctgcggg atcgcccagg gtaatgccga tgcgcgggtg catgcggcgc
     5761 aatgtcagca gcgacgcctt cgctggcaac catggagacg ttttgctttg tgaaacgcgg
     5821 taccgccgat gcccgcgcct gacagagcgc ggcgccgaca ggaaccggca ggcttagaag
     5881 gttttaatgt aggctttcgc gcgcaggctg gcgagccagt gttcctgcaa ctgctgcgcc
     5941 tgctcctgcc gcaatctccc ctcgatgtcg gcgcgcacgt ccttgagcgg ttttgcttcg
     6001 cccccctgtc gcgcctccac tttcagaatg taatgattgc cgccgatatc gacgaccttg
     6061 ctgatctctc cgggcttgag gctaaaggcg acttcggaga gcggcgcgga gagggtcttg
     6121 tcgtcgatcc agccccaatc cccacccaga tcccgcgtgc tgtcttccga atacatctgc
     6181 gccatcttgt cgaaatccgc accggtgatg agcttggcgc gaatctcctc cgccatcgat
     6241 ttttgcgccg cgccgccgcc ggaatcgaga tgtcccggga tcatgatcat ccggagtttg
     6301 acctgcgcct tgctcgagaa ttggtcgcgg tgctggcggt agtattcgtc gatcttcgcg
     6361 ggcgggacga ggagattcga cttcacattc ttggagcgca tcgcctggat gatgattttg
     6421 tcccgttcca gatcgcggaa cttcgacatg gtgtacttct gcgcttccat cgtcttgatg
     6481 aacgcattgc ggtcgccgcc gaagttatct ttaattatcg cgtcgacgcg ttcatcgacg
     6541 aagtgcgagg ggatggcgaa tttctccttt tcgaaagcct ggataatgag ctggcgatcg
     6601 atcaaatcct gcaaagccgc cttgcgcaga tccgtgattt ttttgaccaa ttcatcgccg
     6661 gaatactgtg agcggagcaa acgctcgcgc ggctcgcaga catcgcgcac ttcggaataa
     6721 gtgatgacct tgtcgtttac caccgccgcg atgccattga ccgactccgc gccgagagtc
     6781 ggagcggtgt tcgctaacgc gaggcaggag aaggcgagga gcgtcgccat cggataacgc
     6841 caaatgaagt ttaaagttgc tttaaccatt gccacacctc agggagattg agatcgatcc
     6901 taccggaagt taaacgagga aatttgccgt cgacaaggat aaagtcgccc cgccggctga
     6961 gcatcaattt gtcctcacgc acctcaaccc gcgtgacctg accgcgggcg gcagcaagct
     7021 tgatttcgct gaggaggagc aaattgtcaa ccgctggcgg ccactttccg aagcgatcgc
     7081 gccagtcgcg ccgcagccgc tggagcattt cgacggtcgc gatctcggcc agattccggt
     7141 aggcttggat gcgcagcgcg gcgtcgctga tatatgcggt cgggataaac gctgggatga
     7201 gccgggcggg gccgacggcg agatattcgc cttcgttcgt gccgacgaaa tcaagccgca
     7261 cctcgacttc gaggcggggc cgaagctttt cgcctttcag ctgggcaacc gcttgtttca
     7321 agagctggca gtacagatcg aaacccaccg cgacgatatg accgctttgg gcggtgccga
     7381 gaatgctgcc cgcgccacga atctccaggt cgcgcatcgc gatgcggaag cccgcgccta
     7441 acgagctgta ttgctgaatg gcattgatcc gtttgcgcgc cgcgccgacg gtcatcaact
     7501 cgcgcgggag aagcagatag gcataggctt tgtgctccgc gcgcccgacc cgtccccgca
     7561 attggtaaag atcggccagc ccgaaacgat ccgctcgatc gatgacaatg gtgttcgcat
     7621 ttggaatatc gagcccgctc tcgataatcg tggtgcaaac cagcacgtcg atttttcccg
     7681 cgacgaagcg ctgcatgacg gcttcgagtt catccgcgtc catctgaccg tggccaatct
     7741 cgacccgggc gcccgggcaa agttccgcga tccggtcgcg cactttgccg atcgacacca
     7801 cgcggttgtg gaggaagtaa acctgcccct gacgctcgag ttcgcgctgg atcgcgtcgc
     7861 ggatgatgcg ttcgtcgtag ccgcaaatca ccgtctcgac cgggaggcga ttgagtggcg
     7921 gcgtctcgat cgtcgacatg tctttgaccc cgactaacga taagtaaagc gtgcgcggaa
     7981 tcggcgtcgc cgagagcgtg agaacgtcga cgagcttgaa gagttctttg aacttttcct
     8041 tatgcagcac gccgaagcgc tgttcttcgt cgatcaccac caggccgaga tccttgaaaa
     8101 ccacatcgcc ggagatgagc cggtgcgttc cgacgacgac atcgacgccg ccctcgcgta
     8161 attgctcgag caccttgcgc tgctccgggg gggagcggaa acggctcagc atttcgattc
     8221 gtaccgggta atcgagcatc cgctgccgaa aggtctcgaa atgctgttgt gcgagcaccg
     8281 tggtcggcgt gaggacggcg acctgtttgc cttccatcac cgctttgaag gcggcgcgaa
     8341 tcgccacctc ggtcttgccg aaaccgacat cgccgcagat caagcgatcc atcgagcgcg
     8401 gctcctccat atcgcgcttc gtctcggcga tcgccttcag ttggtcgggc gtctcgcgga
     8461 acgggaacga atgttcgaac tcgagctgcc acttggtgtc gtggccgaaa gcgtgaccct
     8521 gctgggtttc gcgttcggcc tggagcgcca gcattttgcc cgcgtaatcg aaaatcgagg
     8581 aagcggcgtt cttcttggcg cgcgtccact tcgcgtcggc cagcgaactg agggccggcg
     8641 atttttttcc gacgccgacg tagcgcgaga cgaggtaagc ctgctcgagc gggacatata
     8701 gtttggcttc ttccgcaaac tcgatcgcga gcacttcgcc ggcctccgcg ccgccaaatc
     8761 tttgcaggcc aaggaagcgg ccgataccgt gctccagatg aacgacaaga tcgccttcgt
     8821 tcagttcgct gaaatcaatt tgcgcgcggt gcgttccaaa tttcgcggtt cgctgtaaac
     8881 gccgccgacc gtgcgtcggc gcgcggccaa aaagctccgc ggcggccagg acgacgaggt
     8941 gagctgctgg aaagcagaag ccgcgggcga gggtgccctc gatgaggtcg attccgccta
     9001 acgaaatcgt cgtctccgcc atgatctcgc ggaagcgctc aatctcacct tcggtctgga
     9061 agtaaatcgc gatgcgcgcc ccgttttcct gccaggtagt gagccgggcg aagaactgcc
     9121 gtcgtttcgc ctcggcgatg atgaattccc cggtgtcaaa ttcgcccagc tcgcaatcga
     9181 caaacgcgcc gctgaagtcc tcgatctcgc cctggctttc gatccacccc tcgctaatgc
     9241 cgatatccgc cgcttcgtgc ccctcttcga tcgcaaccgt gagatgtccc gttccaatgt
     9301 aatcgcgcac ccttccactc tgatcatcgg ccgcgcctaa caggagctcg gcctggtcca
     9361 gatcgcgcag cgagacctga gtatcgagat cgaattcgcg cagcgactca acctcgtcgc
     9421 cgaaaaactc ggcgcgcagc ggtgcctgcg cctgccacga aaaaacatcg agaatcccgc
     9481 cgcggacggc gaact
//
LOCUS       DATMNS010000089         6790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1556332, whole genome shotgun sequence.
ACCESSION   DATMNS010000089 DATMNS010000000
VERSION     DATMNS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556332"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..395
                     /locus_tag="VNW28_03280"
     CDS             201..395
                     /locus_tag="VNW28_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HXA08977.1"
                     /translation="MPAKSKKQQMAAGAALAAKRGERKKSSLKGASKSMAESMSQKQL
                     KEFASTKRKNLPTRKKSKKG"
     gene            complement(477..2009)
                     /locus_tag="VNW28_03285"
     CDS             complement(477..2009)
                     /locus_tag="VNW28_03285"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HXA08978.1"
                     /translation="MKLEHWERALPDRGTYWLTRFVILRLLGIVYAVAFLVAATQILP
                     LIGSDGLLPLGLFLERVSQALGGSLGGFLRLPSIFWLNHSDTALGATAWIGVVLSCVV
                     AAGYANALLLAVLWVLYMSFVHVGQDWYGYGWEMQLLETGFLAIFLCPLLDGRPFPRR
                     PPPLVIIWLFRALIFRIMFGAGLIKIRGDESWRNLTALYYHFETQPIPNGLSRWFHFL
                     PRGVLRGGTLFNHLAELVAPGFVFWPRILRTVAGSVIIAFQIVISLGGNLSFLNWLMV
                     VPALACFDDRAWAKILPRRLTIRAEAAAGQAVVSPTMSGVTWAIAALVAALSIQPICN
                     IFSPRQIMNTSFDRLDLVNTYGAFGTVGKERYNVVFEGTDAAVPDDAADWKPYPYKGL
                     PVALDERPPQVAPYQLRLDWQMWFAAMGSPNEYPWTLNLIWKLLHNDPGGVGLFRSNP
                     FPQKPPRYVRAVWYRYSFVRPNPQGLWWKREKLGLWLPPLAADDPDYKQLLRQAGWLH
                     GY"
     gene            complement(2252..2764)
                     /locus_tag="VNW28_03290"
     CDS             complement(2252..2764)
                     /locus_tag="VNW28_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HXA08979.1"
                     /translation="MSTEAEPNANAKELLDTMLGYLGFVVEIEETRSEGGNPTLQIYT
                     EEADRLIGREGETLEAVQFLLNRVLQAKNPDAPKWVVDCEMYRSMRQDKIVHHVRQLA
                     ERVRLSGRPLQLEPMNSYERRIVHEAFKDDPEIGTWSPSDSARIKQITLVRRPAKKES
                     APSEKSPDPA"
     gene            complement(2764..4500)
                     /gene="yidC"
                     /locus_tag="VNW28_03295"
     CDS             complement(2764..4500)
                     /gene="yidC"
                     /locus_tag="VNW28_03295"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HXA08980.1"
                     /translation="MDRQAWIAITLCIIGLIAWQVYMVQHPAPVPAKIVASPSRSATP
                     APAPAAAAPGPTGSVPPNESSPTPAPAAFVEQTTTLRNADLELLLTNRGGGIAEAVLP
                     KHRGENGQPVKLNALRRMPIGAIVEKPAAPMFEEFSVVPGQGSVQFERSLPHGVTLRK
                     KFSLPAPPNESDNYIAQMEIDFQNNGPAAYSNPGYFIALGSAAPIHRNDLSNYTRLTW
                     CIEGKTKSTDVTWFAAQTYPFVGVEKRAAQEFFDERITGGEWLGVSNQFFVTLITPLG
                     TKATEAWARRFEARKKDDGQPEYGIEGTMGMPGFKVQPGQTATVQFQLYVGPKLYHRL
                     AQLTHDEAEIMDFGLWKLVSEALLNMMNLLHRLLGNYALAILVLTAIIKLILWPLQTK
                     ANKSMRRMAALSPKMQELKEKYKDDPTKMNAEVMKMYKEYGVNPVSGCLPMMIQIPIF
                     FGLFTMLRQAVELRNASFLWVHDLSQPDTIAHIPGLGWPINILPLIMAGTSFWMTHIT
                     PKSGDATQQRVMMFMPLIFVVFCYNFAAALALYYTTQNLFTILQLYQNRNQPVPKLEK
                     VNQPAKRKGKRR"
     gene            complement(4565..4837)
                     /gene="yidD"
                     /locus_tag="VNW28_03300"
     CDS             complement(4565..4837)
                     /gene="yidD"
                     /locus_tag="VNW28_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HXA08981.1"
                     /translation="MVPLVRFLIRAYQWTLSPLLSWLGGPSTGCRFHPTCSAYLLEAV
                     ERHGPLRGSWLGLKRLARCHPWGGQGEDPVPDGKTFNVQRPTFNVQ"
     gene            complement(4840..5181)
                     /gene="rnpA"
                     /locus_tag="VNW28_03305"
     CDS             complement(4840..5181)
                     /gene="rnpA"
                     /locus_tag="VNW28_03305"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HXA08982.1"
                     /translation="MPASRRLRLSREFDRVRTEGRTVRGGLLMLGLLEAEGGDALRVG
                     FVTSRRVGGAVVRNRVRRRLREIVRRHQFALMQGLWLVVIARPAAACASSAALEDEWL
                     RLTKRASILRP"
     gene            5298..>6790
                     /locus_tag="VNW28_03310"
     CDS             5298..>6790
                     /locus_tag="VNW28_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXA08983.1"
                     /translation="MNLRSFVAAALFLVMAAPFSTAEERGTGRHVVVVVWDGMRPDFV
                     NDQHTPTLAALARSGVFFKNNHPAFPSSTNVNGAVLATGAEPAHNGIVANQEFRPEVD
                     PQKPFDTSDFAALDDPDARISRKFIAVPTVAEIVQKAGYRTAVAGSKPVAQLSDRTRK
                     RETEVAKKSVVVYGGKVIPASLEAVITAAIGPIPPRKAFPNQGEDDWTTRALTDVLWK
                     AGVPKFSLLWLSEPDLSQHETAPGSPTSLAAIKCSDDNLARVLDALKTKHALANTDVF
                     VVSDHGFSTVDLAVDVAARLRAAGFDAVRAFVDKPKPGQILVVTLGGSVEFYVADHDA
                     AIIGKLADFLQRSDFAAVILTRSAQAGTFTFEQAQVNAPNAPDVLVACRWNNQPNQFG
                     IAGQIASDVGKNVGQGNHGTLSPHDMNNTLIASGPDFRRGWTDEIPSGNIDVAPTVLS
                     MLGLKTLEAMDGRVLVEALADSKPVPAVRTQELETQSDLGDFTWRQTL"
BASE COUNT         1382 a         2058 c         2136 g         1214 t
ORIGIN      
        1 ccgccgattt tcacgacctg ccaatccgag gcggcgtgac cagcggtggt tagaaggcag
       61 aggagcgcga gagtcgcgat gaatttggac agggcgaaca tggaccggca ttatgaggca
      121 ttcgcgcgct ggcggcaaca aaatcctttt gtgccataaa aaatgaaggc cgcgcttagg
      181 cccccttacg taatattggc atgccggcaa aatccaagaa acagcagatg gcggccgggg
      241 ccgccctcgc cgccaaacgg ggcgagagaa agaagtcatc actcaagggc gcctcgaaaa
      301 gcatggcgga atcgatgagt cagaagcagc tcaaagagtt cgccagcacg aagcgcaaaa
      361 acctgccgac gaggaaaaag agcaagaaag gctgacgaat ctcctcaagc gcagccggtc
      421 gatatggcgg ccttagaagc ggtagccgct tcgctgtgcg aagcgcgggg gtgaattcag
      481 tagccgtgga gccagcccgc ctggcgcaaa agttgtttgt agtcaggatc atcggccgcg
      541 agcggcggga gccagagacc aagcttttcc cgcttccacc acaaaccctg tggattgggc
      601 cggacgaacg agtagcgata ccagacggcg cggacgtagc gcggcggttt ctgcggaaac
      661 ggattggaac gaaaaagccc gactcctccg ggatcgttgt gaagcagttt ccagatcagg
      721 ttcagcgtcc agggatattc gttaggcgaa cccatcgccg caaaccacat ctgccagtcg
      781 agccgcaatt ggtagggcgc gacttgcggc gggcgctcat cgagtgccac cggcaggcct
      841 ttgtagggat aaggtttcca atcggccgcg tcgtctggga cggccgcgtc ggttccttcg
      901 aagacgacgt tgtaacgctc ctttccgacc gtgccaaagg cgccgtaggt gttgaccaga
      961 tccaaccggt cgaacgaagt attcatgatc tggcggggcg agaaaatgtt gcaaatcggc
     1021 tgaatgctaa gggccgcgac gagcgcggcg atcgcccagg tcacccccga catggtcggc
     1081 gaaacgacgg cctggcctgc ggccgcttcc gctcttatcg tcaggcgacg cggcaggatt
     1141 ttcgcccagg cgcggtcgtc gaagcaggcg agcgcgggca cgaccatgag ccagttcaag
     1201 aacgaaaggt tgccgccaag ggagatcaca atctggaatg cgatgatcac gctccccgcc
     1261 accgtgcgca agatgcgcgg ccagaagaca aacccgggcg cgaccagctc ggcgagatgg
     1321 ttgaaaagag tcccgccacg cagaacgccg cgcggcagaa aatggaacca gcggctgagc
     1381 ccgttcggga ttggctgcgt ctcgaagtgg taataaagcg cagtcaaatt ccgccagctc
     1441 tcgtcgcccc ggatcttgat cagcccggcg ccgaacatga tgcggaaaat gagggcgcga
     1501 aaaagccaga tgatgacgag cggcggtggg cgtcgcggaa aaggccggcc gtcgagcagc
     1561 ggacagagaa agatggcaag aaagccggtc tcgaggagct gcatttccca gccataaccg
     1621 taccaatcct gcccgacgtg gacgaacgac atgtagagaa cccagagcac tgcgagcagg
     1681 agtgcgttcg cgtagccggc cgccacgacg caggaaagga cgacaccgat ccacgcggtg
     1741 gcgccgagag cggtatcgga gtgattgagc cagaagatcg acggcaagcg gagaaatccg
     1801 cctaacgagc cgccaagcgc ttgactgacc cgctcgagga aaaggccgag cgggaggaga
     1861 ccatcggagc cgatgagcgg caggatctgg gtggcggcga caagaaaagc gaccgcgtag
     1921 acgattccga gcagtcgcag gatgacaaaa cgcgtcagcc agtaggtgcc gcgatcgggc
     1981 agagcgcgct cccagtgctc caatttcaca cgagtacgat tgtcgccgtg cgccagggcg
     2041 gattcaagat gcggcggcga gcgccgcggc tggtgtcggc cgaccgatgt cgacacggtc
     2101 ctcatgccgc cggacccgaa gtaattttgt ccgagggtta aatgcgaagg cgcgcggctt
     2161 cggcgacgcg ccttttgcat ttctggcgaa gggatacgcg gcggcgcgtc ccggacatgc
     2221 ggaagcatgt ccctccggca ttcgcgattc gttaggcggg atcgggcgac ttctcgctcg
     2281 gcgcggattc ctttttcgcc ggacgccgga cgagcgtgat ctgcttgatc ctggccgaat
     2341 cggaggggct ccacgtccca atctccggat cgtccttgaa ggcttcgtgc acgatgcgcc
     2401 gctcgtagga attcatcggc tcaagttgga ggggccggcc ggaaaggcgg acccgttccg
     2461 cgagctggcg cacgtggtgc acgattttat cctggcgcat cgagcggtac atttcgcaat
     2521 cgacgaccca cttcggcgcg tcgggatttt ttgcctggag cacgcggttg agaaggaatt
     2581 gaaccgcctc gagcgtttct ccctcgcggc caatcaatcg gtccgcttcc tcggtgtaaa
     2641 tctgcagcgt cggattgccg ccttcgcttc gggtctcctc gatttccacc acgaaaccga
     2701 gataaccgag catcgtgtcg aggagctcct tcgcgttggc gttgggttcg gcttcggtgc
     2761 tcatcagcgt cgttttcctt ttcgtttcgc cggctgattc actttctcca gtttcggcac
     2821 cggctggttc cggttctggt acaactgcag aatcgtgaac aggttctgag ttgtataata
     2881 caaagcgagg gcggctgcaa aattgtagca gaagacgacg aagatcagcg gcataaacat
     2941 catcacacgc tgctgcgtgg cgtcgccgct tttcggagtg atgtgcgtca tccagaagct
     3001 ggtcccggcc ataatcaaag gcagaatgtt gatcggccag ccgagacccg gaatatgcgc
     3061 gatggtgtcc ggctgcgaga ggtcgtgcac ccagaggaag ctcgcgttgc gcagctcgac
     3121 cgcctgccgc agcatggtga agagcccgaa gaagatcgga atctggatca tcatcggcag
     3181 gcaaccgctg accgggttca ccccgtactc tttgtacatc ttcatcacct cggcgttcat
     3241 cttcgtcggg tcgtccttgt acttttcctt cagctcctgc atcttgggcg agagcgcggc
     3301 catgcggcgc atcgacttgt tggccttcgt ctgaaggggc cagaggatga gcttgatgat
     3361 tgcggtcagc acgaggatgg cgagggcgta gttgcccagc agccggtgca acagattcat
     3421 catgttgagg agcgcttcgc tgaccaattt ccagagaccg aagtccatga tctcggcctc
     3481 gtcgtgcgtc agctgcgcga gacggtggta gagcttcgga ccgacgtaga gctggaattg
     3541 cacggtcgcg gtctggcccg gctggacttt gaatccgggc atgcccatcg tcccttcgat
     3601 cccgtactcg ggctggccgt cgtctttttt ccgcgcctcg aaccgccgcg cccaggcttc
     3661 cgtcgccttc gtcccgagcg gagtaatgag cgtcacgaaa aactggttgc ttacgccgag
     3721 ccattccccg ccggtgattc tctcatcgaa gaattcctgt gccgcgcgtt tctccacccc
     3781 gacgaaggga taggtctgcg cggcgaacca ggtgacatcg gtcgacttcg tcttgccctc
     3841 gatgcaccac gtcaggcggg tgtaattaga cagatcgttg cggtggatcg gggccgcgga
     3901 cccgagggcg atgaaataac cgggattgga gtaggccgct gggccgttgt tttggaaatc
     3961 gatttccatc tgcgcgatgt agttgtcgct ctcgttaggc ggcgcgggca ggctgaactt
     4021 cttgcgcagc gtcacgccat gcgggagcga ccgctcgaat tgcacactcc cctgccccgg
     4081 cacgaccgag aattcctcga acatcggcgc cgcgggtttc tcgacgatcg cgccgatcgg
     4141 cattcgccgg agcgcgttta acttcaccgg ttgcccgttt tcgccgcggt gcttcggcaa
     4201 gacggcctcg gcgatgccgc cgccgcgatt cgtcaggagc agctcgagat cggcattgcg
     4261 cagcgtcgtc gtctgttcga cgaaagccgc cggagccggc gtgggggacg attcgttagg
     4321 cggaacggac ccggtcgggc ccggggccgc ggcggccgga gcaggagctg gcgtggccga
     4381 acgactgggg gaagcgacga ttttcgccgg cacgggcgcg ggatgctgca ccatgtaaac
     4441 ctgccacgcg atcagcccga tgatgcaaag cgtaatggca atccaggctt gtctgtccat
     4501 aaactgattg gcgtcgatta ttcacggaca tgcgattgcc aatggccgat tgccagttgc
     4561 caattcattg aacgttgaac gttggacgct ggacgttgaa cgttttccca tccgggaccg
     4621 ggtcctcccc ttgcccgccc catgggtggc aacgcgcgag tcgtttcagc ccgagccagc
     4681 tcccgcgcag cgggccgtgg cgttccaccg cctcgagaag ataggccgag caggtcggat
     4741 gaaaacggca acccgtggac ggcccgccga gccaggagag gagcggcgag agggtccatt
     4801 ggtaggcgcg gatgaggaaa cgaacgagcg gcaccatcgt caggggcgta ggatagaagc
     4861 gcgctttgtg agacgcaacc actcatcttc cagcgccgcc gagctggcac aggccgccgc
     4921 cggccgcgca atcacgacga gccagagccc ctgcatgaga gcgaattgat gacgccgcac
     4981 aatctcccgc agccgccgcc gcacccgatt ccggacgacc gctccaccga cccttcgtga
     5041 agtaacaaac cccacccgga gcgcatctcc gccttccgcc tcgagcaggc cgagcatgag
     5101 gagcccgccc cgcaccgtgc ggccctcggt ccggacccgg tcgaactcgc gactcagccg
     5161 caaacggcgc gacgcgggca atcggcagcg cggaggtttt ggggtcgcat cgtccatccc
     5221 gtggccagct taacacaccg caaaagtcac acgaatgcca ctcgaattcc ggcgggcgcc
     5281 atgccaactt ccccacgatg aatctccggt cattcgtcgc cgcagcgctc tttctcgtca
     5341 tggcggcgcc tttctccacc gccgaagaac gcggcacagg caggcatgtg gtggtggtgg
     5401 tgtgggacgg aatgcgaccg gatttcgtca acgaccagca caccccgacg cttgccgcgc
     5461 tggctcgctc cggagtcttt ttcaaaaaca atcatcccgc ctttcccagc tcgacgaacg
     5521 tgaatggcgc ggtcctagcg accggcgcgg agccagccca taacggcatc gtcgcgaatc
     5581 aggagttccg gccggaagtc gatccgcaaa agccattcga cacctccgat tttgccgcgc
     5641 tcgacgatcc ggatgcgcgg atcagcagga aattcatcgc tgtgccgaca gtcgccgaga
     5701 tcgtccagaa agcgggctat cgcactgccg tggccggatc gaagccggtg gcgcaactca
     5761 gcgatcggac ccgcaaacgc gagacggagg tcgcaaaaaa gtcggtcgtc gtttacggcg
     5821 gcaaagtgat accggccagc ctcgaagccg taatcacggc agcgatcgga ccgatcccgc
     5881 cccgaaaagc tttcccaaac cagggcgaag acgactggac aacccgggcg ctgaccgacg
     5941 tgctttggaa agccggagtg ccgaagtttt cgctcctttg gttgagcgag cccgatcttt
     6001 cgcagcacga aaccgccccg ggctcgccta cttcgctcgc cgcgatcaaa tgcagcgacg
     6061 ataacctcgc aagagtcctc gatgccctga aaacgaagca cgccctggcg aacaccgacg
     6121 tcttcgtcgt ctccgatcac ggattctcga cggtcgatct cgcggtcgac gtggcggcgc
     6181 gtttgcgcgc ggcgggtttc gacgcggtcc gcgcctttgt cgataaaccg aagccgggcc
     6241 agatcctcgt cgtcaccctc ggcggcagtg tcgaattcta cgtcgctgat catgacgcgg
     6301 cgatcatcgg caagctggct gatttcctgc agcgctccga cttcgccgcc gtcatcctga
     6361 cccgcagtgc gcaggccggc actttcacgt tcgaacaggc gcaggtgaac gcaccaaatg
     6421 ctccggacgt cctcgtcgcc tgtcggtgga ataatcagcc taaccaattt gggattgcgg
     6481 gacagatcgc gtccgacgtc ggcaagaatg ttggccaggg caaccacggc actttgagtc
     6541 cgcacgacat gaataatact ttgatcgcga gcgggcccga tttccggcgc ggctggacgg
     6601 acgaaattcc gagcggcaac atcgacgtcg ccccgacggt cctttcgatg ctcggcttga
     6661 aaacgttgga ggcgatggac ggccgcgtct tggtggaggc actcgccgac tcgaaacccg
     6721 ttccagcggt caggacgcag gaactcgaaa cgcaaagtga tctgggcgat ttcacctggc
     6781 gccagacgct
//
LOCUS       DATMNS010000090        24497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_126962, whole genome shotgun sequence.
ACCESSION   DATMNS010000090 DATMNS010000000
VERSION     DATMNS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 24497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24497
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126962"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2353)
                     /gene="ppdK"
                     /locus_tag="VNW28_03315"
     CDS             complement(<1..2353)
                     /gene="ppdK"
                     /locus_tag="VNW28_03315"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HXA08984.1"
                     /translation="MAKKAKSPRSKSAPKRQIKPAKARRSVYYFGDGKADGGGSMKPL
                     LGGKGANLAEMTRIGLPVPPGFTITTEVCTYFSANKRTYPRGLETEVEAALAKVEKSV
                     GKKLGDKERPLLVSVRSGARDSMPGMMDTILNLGMNDTVVEIVAQKTNNPRFAWDSYR
                     RFLQMYGDVVMGVQKRAGEDHEPFESVIEHLKDQRYGNHHFPDVQLTVDDLMELVRRF
                     KALIKERTGKSFPQDPRKQLWGSIGAVFGSWNNDRAVVYRRKYGIPHDWGTAVNVQTM
                     VFGNMGDTSATGVAFTRDPASGEKVFYGEYLINAQGEDVVAGVRTPHPIAKLAEEMPE
                     AHKALMKVRALLEKHFKDMQDLEFTVEDNRLYILQTRNGKRTGLAAVRIAVEMVEERL
                     ITKEAAIKRIPADSLGHLLAPIFDRKSIAGAKKIGGGLAAGPGAASGHVVLSAEEAVR
                     RAHRGEKVVLARVETSPEDLRGMIAAEGILTSRGGVSSHAALVARQMGKVCVCGASGI
                     EIDYQKRTLSANGVTLKEGDYLSIDGTAGEVFAGEVTTAPSEIVQVLVDRSLDGKKSA
                     TYQQYAKLMSWVDRARKLGVRTNADTPEQVENAIAFGAQGVGLCRTEHMFFEGDRIDA
                     MREMILAEDKEARVKALAKLLPYQRQDFAGIFKALDGLPATIRFLDPPLHEFLPHEKE
                     QQLDLAKKMNVPAETISRRVNELHEFNPMLGHRGCRLGITFPEISEMQARAVFEAAAE
                     VQKKGKKVRPEIMIPLVGFPKELKLQIDIVHRVAAKVAKERKTK"
     gene            complement(2465..2665)
                     /locus_tag="VNW28_03320"
     CDS             complement(2465..2665)
                     /locus_tag="VNW28_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HXA08985.1"
                     /translation="MDSIIETRELQIERKLFSIDLRENGRGRFLRITEDSQGHRNVII
                     VPMSGVDDFADAIDDVLASEPA"
     gene            complement(2726..4897)
                     /gene="rnr"
                     /locus_tag="VNW28_03325"
     CDS             complement(2726..4897)
                     /gene="rnr"
                     /locus_tag="VNW28_03325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HXA08986.1"
                     /translation="MKRVADTKQRILALLGTPKYRPLDEAELARALGFKSEGRNGVKE
                     ALRELERAGEIARIRKHRYILPAEADLATGRLQIHQAGFGFLAREGSDEGDIFIAAEN
                     TGTAMNGDRVVARITRDADYARARSGSNRPEGRVIRILERAHDTIVGTLQQTRNFFYV
                     VPDDPRLVHNVYVQPRPLPGAPRSPAPEDKVVVRLEAWESRHVNPEGEIIELLGRATD
                     PGVDMLSIIRKNDLALEFPAAVLAEANKISGTVDPARFGEREDLRGKFIVTIDPDDAR
                     DFDDAINVEQLAGGEWELGVHIADVSAYVTPGSALDREALHRGNSVYLVDRVIPMLPE
                     RLSNGVCSLNPNVVRLTHSVFIRFARNGAPERARFARTIIQSACRLTYKEAYALLQKS
                     PNDDLSHRLHTAWNLASILRRRRFAHGSLDLDFPEVKVIVDKQTGRPLRLERIENDES
                     HQLVEEFMLAANEAVAAELKNRLLPTIYRVHENPDPEKLAEYRQFVISYGFNAGDLTH
                     RRELQRLLASFDGKPEEQALKIGLLKSLKRARYATQPLGHFGLAKNNYAHFTSPIRRY
                     ADLVVHRTLAERDGKRGARGSAGELAGIAEHISTTERVAAEAENESVKMKKLEFLQRQ
                     LTEKNPQVFRAVVIEVRNYGLLIELPDVLLTGLVHVSSLNDDFYIFDGARRRLLGRQS
                     KRILAVGDVLKVVVARIDVFKRQADFAIVDDRPAKPRRRGR"
     gene            5048..5893
                     /gene="folP"
                     /locus_tag="VNW28_03330"
     CDS             5048..5893
                     /gene="folP"
                     /locus_tag="VNW28_03330"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXA08987.1"
                     /translation="MEATRWQIGDKVLDLTARARLMGVLNVTPDSFSDGGEFFTTEAA
                     VKHGREMADHGAEMIDVGGESTRPGARPVAPDEEMERVLPVIRQLASSVAAAISIDTS
                     KAVVARAALAAGATIINDVTGGQRDPEMFALAAERRAALIIMHMQGTPETMQLDPTYG
                     DVVDEVADFFRQQFAQAVRCGVNPMCIAFDPGIGFGKTVAHNLELLANLPRLQVENRP
                     LVVGVSRKSFLGKISGLPSDRASATVAMTSLLRARGAHVLRVHDVLTNASALRTTEAL
                     LAATR"
     gene            5890..6765
                     /gene="cdaA"
                     /locus_tag="VNW28_03335"
     CDS             5890..6765
                     /gene="cdaA"
                     /locus_tag="VNW28_03335"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HXA08988.1"
                     /translation="MIHEFTLLWFDKWRSAVEILLLSVVIYYGYLYFRGTRGAKVLTG
                     LAIVFLTLILISQLLNLVVISFIIRSFSVFLAVALVVIFQPELRRALAELGGHPIFSL
                     TSEKRETVHDLAEVVTQLSNKQFGALIAIERDTSIKIYEETGVIVDAAFSTELLLTLF
                     HPKSALHDGGVILRNGRIAAAACIFPVSQRETLDRSIGLRHRAALGITEESDAVAVVV
                     SEETGAISICHRRRLERNFSPETFRQRLGEILLHRTHEETDPEQLAREADVPPARDRA
                     LVPHQEERNNDTLAV"
     gene            complement(6809..7261)
                     /locus_tag="VNW28_03340"
     CDS             complement(6809..7261)
                     /locus_tag="VNW28_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08989.1"
                     /translation="MAKKTKRKLARPTLPLQRQLNLRHEGRHFDLRHIFDRVNHRHFR
                     GRLRGYKVLWGRRRKERPKEYFIFGSIQEEDRVIRIHPLLDQPFVPRWFLEYVLYHEM
                     LHSVVPEETDATGRRRVHTEEFYRRERAFPRYRRARRWEDQNLARFLR"
     gene            7479..8591
                     /locus_tag="VNW28_03345"
     CDS             7479..8591
                     /locus_tag="VNW28_03345"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HXA08990.1"
                     /translation="MTPFLRKLLGLNWLLIVNMLALAIFGVVAIYSATYMRQDPAAAE
                     FWRKQANWVAVGFFAFIVTSLIDYRWIKWGALPMYLAGLFFLILTRFIGTKVYGSRSW
                     LHLGPINFQPAQLAVVAGIMVLALFLSQFRQMHPMLRLTLCGAIVGAPCLLILMQPDL
                     GECMIWGPVLLALLFVGGLPLRYLICVVLIAVAFIPIAYVELKPYQQERITAFINPDI
                     DRQGSAWAINQSLIAIGSGGWSGKGFKAPNTQIELGFLPATAVHNDYIFSAIGEQWGF
                     VGGIALLGAFGLMLLTCLFVAYKASDLFGLLLVIGISTLIFTHIFQNVGMTISILPIT
                     GVPLPLISYSGSFALMIMFGLGIVNSVWVHRKVPVR"
     gene            8652..9089
                     /gene="secG"
                     /locus_tag="VNW28_03350"
     CDS             8652..9089
                     /gene="secG"
                     /locus_tag="VNW28_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HXA08991.1"
                     /translation="MSILIDSLLVFEALVALLMMLFILMQRPKSEGLGAAFGGGVTEN
                     IFGAQTTNVLTTITTWLASIFFIVVLALSVLYAHRGAPDSAVRRELMKTSAAAKAAAP
                     LPAKAAAKTAAADAKQKVAPEAKASVPPAKTAPSPAASAQPKP"
     gene            9159..11987
                     /locus_tag="VNW28_03355"
     CDS             9159..11987
                     /locus_tag="VNW28_03355"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXA08992.1"
                     /translation="MTSTQQRTLASANCEIAFDDRTRRLYATDASLYQIEPAAVAFPR
                     SAEQARDVILASGSAGISVSPRGAGSGLSGGALGDGLIVDFSRHNRQISALNLEQRTV
                     RVGAGVVLDQLNAFLEPHGFCFGPDVASSSRATLGGMIANNSSGAHVPVYGTTADHVR
                     ALEIVLADGRTETIGADRPALAAEQEMIARLVRAQSAEIAERMPPGMLKRWCGYALER
                     FARAPANLIEILSGSEGTLAAILSGELRIVPLPRAKGLGLIFFASVGEAMHATVELLD
                     LRPAAIEHMDRPLFEQTKGQRNFAAARSLLELDTKPCEAILVVEFFDDVADRLAAVAA
                     RKLGLRTLICADEAEMNLVWSMRKAGLSLLTGRPGAAKPVAFMEDAAVRPAQLPAYVA
                     GLEAIMQPLGLDVCYYGHAAAGLLHVRPVLDLHTAADLKKYRQVADEVAALVRQFKGS
                     LSAEHGVGMARTEYLRGQVGPELFEVMRAIKRVFDPANLFNPEKLIGDGRFRIDQDLR
                     ENFRRPIKLPFTPVLAFAAKDHSFIGNLEQCNGCGGCRKETPTMCPTFLATGEEYMST
                     RGRANAIRAALDLRVTGGDPLRAQILEDALSNCLSCKACTTECPSNVNLALLKAELIH
                     ARHERDGLPWQTRILSAVDLLGRLGCTLPALANAALDSPPLRAEMEKTLGLAAQRSLP
                     HYAEERFDRWFVRHARPTDGKPRGRVIIWDDTFMRYHEPHIGIAAVKVLEALGFEVTL
                     PTGRRCCGRPAFSLGNLQEAVRLGRHNLHLLQRSGETTPILFLEPSCYSMFVEDYREL
                     NLPETEAVARRCFLFEKFVDDLLEREPDAIRFAQRAVPVAIHAHCHAKSLLDPGFMVR
                     LAERLPGRKATLLETGCCGMAGAFGMLKAKYDLSLKVAAPLVELVIDQPVDAVVVASG
                     TSCRHQIDDLTPARPRHMAELLADAIAR"
     gene            complement(12000..12731)
                     /locus_tag="VNW28_03360"
     CDS             complement(12000..12731)
                     /locus_tag="VNW28_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXA08993.1"
                     /translation="MDYGETQRIHDTHAAIVREKSEPRVGHEPLSFWLITIYGLAVFF
                     GGAYLGRYAGNFSGDGLDYLGGAPRVATKAGAAGGAEQVAELTPVQKGQKIFSANCAT
                     CHQANGLGVPGQYPPLAGSEIVNGGGRRPVMIVLKGLQGPLTIKGQHFGTAVMQPWEK
                     TLNDQKIAEVLTYVRQEWGNTGGPIAPEGVGALRKELAGRTESWTEPDILAVPVDADL
                     PGGAPPAPGTAAKPGASPPAAPPKP"
     gene            complement(12767..13678)
                     /locus_tag="VNW28_03365"
     CDS             complement(12767..13678)
                     /locus_tag="VNW28_03365"
                     /inference="COORDINATES: protein motif:HMM:NF014486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit II"
                     /protein_id="HXA08994.1"
                     /translation="MKGLQPLFLGIFGIFAFSWLGMTVVPNLQIGSLSPQTDEDGGDV
                     YPAPPSGMTLRGAQVYAANGCVYCHSQQVRPDYGGADLERKWGDRRSAPRDYIFQPIV
                     FLGKMRTGPDLANVGHRSMPEAKGATPAPAAPAAPATGAAVSAAGAPAATASPAAAAA
                     AAASPAGTSAPAPAAVPVANAPTPAPSLVANASELTADGKPLPYTAAWHHRHLYNARS
                     LSEDSNMPPFRFLYEKRRIAGSASPDAINFAGDPDEEPAAGWEIVPSYDAKCLVAYLM
                     SRDQSHPLKEAKAPATIAPAPAKEPQK"
     gene            complement(13678..15165)
                     /locus_tag="VNW28_03370"
     CDS             complement(13678..15165)
                     /locus_tag="VNW28_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXA08995.1"
                     /translation="MSQPAALTQEPPLGDVEVTTSDVERVERALIDASTRLPVLVFYA
                     SAIAWLLLATFFGVLTSFKMHVPGFLGDVSFLTLGRVGPAHFNAIIYGWGSMAGMGTS
                     IWLMARLCRTTLRHPLLLISGAAFWNLGVLLGIVGILLGDSTGYEWLEFPGSSASTLF
                     VAYLLIMTWALIMFRYRRRGHVYITQWYLLGAFLWFPWLYAAAQVMLFLLPVQGVMQA
                     AVNWWFANNLLFLWFGSIGLGTAYYMIPKVIGRPVHSYYLAAIGFWTFALFSSWTGMQ
                     RLVDGPFPAWMITASIAATILTIIPVATVGLNHHMTMRGYFPLMRYSPTLRFTVFGAI
                     AYTAFSVVGVIISLRSMARIVQFTEASVAYTYMGLYAFFTMVIFGSMYYIVPRLVGRE
                     WRYASLIKLHFWGSAYGIGLMVLLLLVGGFAQGLSLNDPTLPFSEITDTVLPYLRGRT
                     LSWILLTVSHLVFAFHFGLMLLGFGRTASVPTFLNPLEREPGEGH"
     gene            complement(15356..15808)
                     /locus_tag="VNW28_03375"
     CDS             complement(15356..15808)
                     /locus_tag="VNW28_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08996.1"
                     /translation="MPNDETLIHPEQPRFLLSSWLWAVVLFVFFGVIVAITFGAMHRG
                     SSYEEDRAKTRTEKLKTAREDWNKTLGSYGWVDKGKGVAHIPIQRAMQLELADLQAKK
                     PAPAGPIATPEPAAAPNAPAAAPGTPPAAAPPAAKAGETPPLPRPGQP"
     gene            complement(15811..17013)
                     /locus_tag="VNW28_03380"
     CDS             complement(15811..17013)
                     /locus_tag="VNW28_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA08997.1"
                     /translation="MSDRSHVLPAPEGEYFENGRFAGLSALCGVIGLIGLVLSFIGAF
                     VSPVQFSFSWLFGFIFFFTLCIGCLFWTIVHHVVDAEWSVVVRRQLENIALLLPLLGV
                     FFLPIWFLRKYLYRWMDVPRGVDPVLDAKRGYLNWEFFLGRAIFFFVALTIVAYLLRS
                     YSVRQDRDGNPRFTILMRKAAFVGLPLFAFCLTFGAFDWLMSLNWHWFSTMWGPYIFA
                     GTAGSSMALLVLVITALRQAGYLEGVVTLEHYHIMGKWMLSFSVFWAYIGFSQYMLYW
                     YANIPEETQYYLIRNTESWNVLSWILVIGRFFVPFAILLLRSVKKQPFVLCCVAGWIV
                     CMQALDMYVIVLPALHGTGVHLSILDFLPLVGIGGTLAFFYLRIIGKTSLFPVRDPRL
                     IESLRIVN"
     gene            complement(17010..17720)
                     /locus_tag="VNW28_03385"
     CDS             complement(17010..17720)
                     /locus_tag="VNW28_03385"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXA08998.1"
                     /translation="MLRAFFLIFILVTIAWIAVFGFRGQKSGKPEIEIFPDMVRQPKV
                     RAQSELDFFADQRGARQPVEGTVPIGYDMPLPDDGGASVDAAGEMAMHPHLGFSEGTD
                     YYNTGKMGENWGTGIPLEVTPALMQRGQQRFNINCAVCHGPLAAGDGIVKQYGLATVV
                     SLQDDRIRKMADGEIFNTITHGKNTMMAYGPRVTVQDRWAIISYLRALQRSQHASAAD
                     VPPEDQAQLEKKAEPEKK"
     gene            complement(17723..18277)
                     /locus_tag="VNW28_03390"
     CDS             complement(17723..18277)
                     /locus_tag="VNW28_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HXA08999.1"
                     /translation="MITPSGNRVYGMAAEYPSAASLYEAAKQVRDAGYKRWDVHSPFP
                     IHGMDDAMGLGKSWLSAVVLCGGLTGLLTACVLTFGPSSYLYPLIVHGKPFNFWTVPA
                     FFPIMFELTVLFSAFAAFFAWQAMNFLPRWNHPMFNWERFKRVTNDGFFLVLEARDPR
                     FSENEARELLERTGGQHITVVHEE"
     gene            complement(18274..19719)
                     /gene="nrfD"
                     /locus_tag="VNW28_03395"
     CDS             complement(18274..19719)
                     /gene="nrfD"
                     /locus_tag="VNW28_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HXA09000.1"
                     /translation="MEGPITAPQASASVASPLARVPLVLNKRNFDWVTNRISGAVENP
                     TPRWWWVAFAITSAIATFGLTCIGYQISTGVGTWGLNSPVGWAWDITNFVFWIGIGHA
                     GTLISAILFLLRQKWRTSINRSAEAMTLFAVICAAIFPGIHVGRVWMAWFLAPVPTQY
                     GIWPNFRSPLLWDVFAVSTYFTVSVLFWYTGLVPDLGTLRDRATSKIKKFFYGIFALG
                     WRGSNRHWRHYEMAYLLLAGLSTPLVLSVHSVVSFDFATSVIPTWHTTIFPPYFVAGA
                     IFGGFAMVLTLMIPARAIFKLQDIITPQHIDKMAKIILLTGSIVGYAYAMEFFIAWYG
                     GNQYEKFAFYNRIFGPYWWYWWVGMLFCNTLSPQLFWFRWCRRNIFVVYFVCMCVNAG
                     MWFERFIIIVGGLHRDFLPSAWGFFTPTWVDIWTFIGTFGIFLSLFLLFIRFAPMIAM
                     SEVKIVLPEADPHYVPPDGQHAVAAGGKEHA"
     gene            complement(19747..23058)
                     /locus_tag="VNW28_03400"
     CDS             complement(19747..23058)
                     /locus_tag="VNW28_03400"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HXA09001.1"
                     /translation="MKRVLQHPAPSKTGKQYWRSLGELSDTPEFRGWLEREFPAGAAE
                     IEGDEVSRRSFLKLMGASMALAGFGLNSCRKPEMHLVPFTKSAEWVIPGKALYYATAM
                     PRRHGAIPLVVSTVDGRPIKIEGNPLHLDSAGATDAFAQASVLDLYDPARSKRFVHKN
                     ELSDRAAFEKYIAELRPKMAANRGAGLAFLVEETYSPTRERLRNELAKQFPKMRWCVY
                     DPLLSEAQHFSTQISFGDNTRLIPQFDKADVVFALDSDFLNCGEGDLASIRGFTARRR
                     VREAKDSMNRLYVVENRFTLTGAMADHRLRYGASQIPAITHALASKIAVATRDPGLSS
                     VLITLKAPTKAEKYNGRWLEEAAQDLLSKPSASLVVAGPHQPVVVQLLVYGINAALKN
                     LGTTLLLRELPRTSRESSILQLASEIDAGRIQQLFIVGGDPVYNALRSITLDRKTKQP
                     LDWGELQKRVPDVVRLGQYEDATSALSQWHLPAAHYLETWGDALTPSGSYVAIQPMIL
                     PLFGGMSELDLLWTVLGQPRVDGPQLVKETFRATSPPGDFETAWSKFLHDGFAAHLPP
                     KDRTPKFNGNSAGGVAHTLWSNDAPAPTKDSPEIVLVGSYAIDDGRYINNGWLQEMPD
                     PITKLTWDNAAMMSPAFAHRTGVKDGDLVQIVVTDTAKDRQGKPIRRELVIAALIVPG
                     HAENSVTIPLGYGRKHTGPVGEEAGFNAYLLRTSSNPHFIIADGKAIASVQVKKVAGT
                     YPLATTQQHFSIEGRGLVREATLEHYRGDEDFVKKIAGDEELPPQLPSLYTHPKLDAP
                     QQWGMTVDLNTCTGCTACVIACQAENNIPIVGKLQVSHGRAMHWIRIDRYYASAKPFD
                     QDKGEWPENPEMVHEPIMCQHCENAPCETVCPVNATVHSEDGLNVMAYNRCIGTRYCM
                     NNCPFKVRRFNFFDYTQRPIGKKKIGGFSVYQEYLGPLTDKGAPDTIKLQKNPNVTVR
                     MRGVMEKCTYCVQRIEEAKIAAHVRAGASDKTLIPRDSFTTACAQACPAEAIVFGDIA
                     DPETRVSKIKAQNRNYRLLEYLNVKTRTSYLARIRNPNPKMPDAGVIAVASLGNEAKP
                     PVENTAPGLPPEPQP"
     gene            complement(23055..23780)
                     /locus_tag="VNW28_03405"
     CDS             complement(23055..23780)
                     /locus_tag="VNW28_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXA09002.1"
                     /translation="MANFFPRWTNWLPLKIMICGTVIVTALVGATWYYDTPKYTRVGY
                     MPTQPVPFPHDLHVTQLGMDCRYCHNAVELTAHSNVPDTQVCMNCHTQVQKENPKLEP
                     VRASWTTGQPVPWVQIHRTPDYVYYNHSAHVNRGISCFSCHGEINHMPVVYDAKPLSM
                     KWCLECHRAPENFLRPEDQITNLDWKPSDVKPAEFVAKYGQPEGMKEDWSKKTNLTQE
                     EIGLTLKERWDIKPPLNCQGCHR"
     gene            complement(24045..>24497)
                     /locus_tag="VNW28_03410"
     CDS             complement(24045..>24497)
                     /locus_tag="VNW28_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09003.1"
                     /translation="NTAFARLTNQLTYCGGRAANHARAEHAMRYLAVPMVAERRGFLV
                     GGILLADRELRAPPFHLTVVGSKHDPKARDLFATAVRQPIPYKRVEWWDPAEGPLPNS
                     DVEYPTFEQAAAFICTDRRCSAPIFDGAKISSFLEANRSRIPVAGHPN"
BASE COUNT         4800 a         7362 c         7564 g         4771 t
ORIGIN      
        1 attttgtttt tctctccttc gccactttgg cggcgacgcg atggacgatg tcgatctgca
       61 gtttcaattc tttcggaaat ccgacgagtg ggatcatgat ttccggtcgg acttttttgc
      121 ccttcttctg cacttcggcc gcggcttcaa agaccgcgcg cgcctgcatc tcggagatct
      181 cggggaaagt gatgccgagg cggcagccgc gatggccgag catcggattg aactcgtgca
      241 attcattgac gcgccgcgaa attgtctcgg ccggcacatt cattttcttc gcgagatcga
      301 gctgctgttc tttctcgtgc gggagaaatt cgtgcagcgg cggatcgaga aaacggatcg
      361 tcgccggcag accatcgagc gccttgaaaa tgccggcgaa atcctggcgc tgatacggga
      421 gcaacttggc cagcgccttg acgcgcgcct ctttgtcctc ggcaaggatc atttcgcgca
      481 tcgcgtcgat gcgatcgccc tcgaaaaaca tgtgctcggt gcggcaaagt ccgacgccct
      541 gcgcgccgaa agcgatggcg ttctcgacct gctccggcgt gtcggcgttg gtccggaccc
      601 caagcttgcg cgcgcgatcg acccagctca tcagcttcgc gtattgttga taggtcgcgc
      661 tcttcttccc atcgagtgaa cgatcgacca gcacctgcac gatctcggaa ggcgcggtcg
      721 tcacttcacc ggcgaaaacc tcaccagcgg tgccgtcgat cgaaaggtaa tcgccttcct
      781 tcaaggtcac gccgttcgcc gagagcgtgc gtttttgata atcgatctcg attcccgaag
      841 cgccgcagac gcagactttg cccatctgcc gcgcaacgag cgcggcgtgc gaggaaacgc
      901 cgccgcggga agtgagaatg ccttcggcgg caatcatccc gcgcaagtcc tcgggggaag
      961 tttcgacgcg ggcgagaacc actttctcgc cgcgatgggc gcggcggacg gcttcttccg
     1021 cgctcagaac aacgtgaccg gaagctgcgc ctggtccggc cgccaaaccg ccgccaattt
     1081 tcttcgcgcc agcgatcgat ttgcgatcga agatcggcgc gaggaggtgg cctaacgagt
     1141 cggctggaat acgtttaatg gcggcttctt tcgtaatcaa acgctcctcc accatctcga
     1201 ccgcgatgcg cacggcagcg aggccggtgc gtttgccgtt gcgcgtttgc aggatgtaaa
     1261 gccggttgtc ctcaaccgtg aactcaaggt cctgcatatc cttgaaatgt ttctcgagga
     1321 gggcgcgtac tttcatcagc gccttgtgcg cttccggcat ttcttccgcc agtttcgcga
     1381 tcgggtgcgg cgtgcgcacg cccgcgacga cgtcttcgcc ctgcgcgttg atcagatatt
     1441 cgccgtagaa aactttttcg ccgctcgccg ggtcgcgcgt gaacgcgacc ccggtcgcgc
     1501 tcgtgtcacc catgttgccg aaaaccattg tctgcacgtt gaccgccgtg ccccagtcgt
     1561 ggggaatgcc gtacttgcgg cgataaacga ccgcgcggtc gttgttccac gaaccgaaga
     1621 cggcgccgat ggaaccccag agctgttttc tcggatcctg cggaaacgat tttccagtgc
     1681 gttccttgat tagcgccttg aagcgcctaa cgagttccat caggtcatcg acggtcagct
     1741 ggacgtcggg gaaatggtgg ttgccatagc gttggtcctt gagatgctcg atcacgctct
     1801 cgaacggctc gtgatcttcg cccgcgcgct tctgcacgcc catcacgacg tcaccgtaca
     1861 tttgcagaaa acggcgatat gaatcccagg cgaagcgcgg gttgttcgtt ttctgcgcga
     1921 cgatttcgac cacggtgtcg ttcatcccaa ggttgagaat ggtgtccatc attcccggca
     1981 tcgagtcgcg cgcgcccgag cgcacggaaa cgaggagcgg gcgctctttg tcgccaagtt
     2041 tcttgccgac cgatttctcc actttggcaa gcgcggcctc gacctcagtc tcgagtccgc
     2101 gcgggtaagt gcgcttgttc gcggagaaat aagtgcagac ctcggtcgtg atcgtgaacc
     2161 cgggcgggac tggcaaaccg atgcgcgtca tctcggcgag gttggcgcct ttgccgccga
     2221 gaaggggctt catgctccct cccccgtcgg ctttgccgtc gccaaaatag tagacgctgc
     2281 ggcgagcctt ggccggtttg atctggcgtt tcggagcgga tttcgaacgg ggcgacttcg
     2341 ctttcttggc catgagaaaa aagggtggcg ctcaaaggcg cgcggaagcg caagctaaag
     2401 cgcgcgcgcg gggtgtcaac gcatccccaa atgcgcagcc tgattttgct ttagcgcgcc
     2461 gcgcctacgc gggttcggag gcaagcacgt catcgatggc gtcagcaaaa tcgtccacgc
     2521 ccgacattgg cacgatgatg acatttcgat gaccctgact gtcttcagtg atgcgcagga
     2581 atcggccacg gccattttcg cgcagatcga ttgaaaagag ctttcgctcg atctgcaact
     2641 ctcttgtttc aatgatgcta tccattttcg acggcgcgga tgttaggctg ggccgccaag
     2701 aaaatggaag aaaattcttt ggaatttaac ggcctcggcg tcggggcttg gccggacggt
     2761 cgtccacgat cgcaaaatcg gcctggcgct tgaaaacatc gatgcgggcg acgaccactt
     2821 tcaacacgtc gccgaccgcg aggattcgtt tcgattgtcg gccgagcagc cgccggcgcg
     2881 cgccatcgaa gatgtagaaa tcgtcgttga gcgacgagac atggactagc cccgtcagga
     2941 ggacgtccgg caactcgatg agcaggccgt agttgcggac ttcgatcacg accgcccgga
     3001 aaacctgcgg atttttctcg gttagctgcc gctggagaaa ctcaagtttc ttcattttca
     3061 ccgactcgtt ttccgcctcg gcggcgacgc gttcggtcgt cgagatgtgt tcggcgatcc
     3121 cggccagttc gccggcgctc ccgcgcgctc cgcgcttgcc atcgcgttcc gccagggtgc
     3181 gatgcacgac cagatcggcg taacggcgga tcggactcgt aaaatgcgcg tagttgtttt
     3241 tcgcgagacc gaaatgaccg agtggctgcg tcgcgtagcg ggcgcgcttt aaacttttca
     3301 gcaacccaat tttcaacgcc tgctcttccg gtttgccgtc aaaagatgcg aggagccgct
     3361 gcagttcgcg gcggtgcgtc aggtcgccgg cgttaaatcc gtagctgatc acgaactggc
     3421 gatactcggc caatttctca gggtcgggat tttcgtggac ccgataaatc gtcggcagaa
     3481 gccggttttt cagctcggcc gcgaccgctt cgttggccgc cagcatgaat tcctcgacga
     3541 gttgatgcga ttcgtcgttc tcgatccgct cgagacgcaa cggacggccg gtctgtttgt
     3601 cgacgatcac tttcacctcc ggaaaatcga gatcgagcga gccgtgagcg aaacggcggc
     3661 ggcgcaggat cgaggcgagg ttccaggccg tgtgcaaacg atgcgagagg tcgtcgttag
     3721 gcgatttttg caggagggcg taggcctcct tgtaagtcag ccggcaggcg ctctggatga
     3781 tggtgcgggc gaaacgcgca cgctctgggg caccgttccg ggcgaagcgg atgaagacgg
     3841 aatgagtcag cctaacgaca ttcggattca ggctgcaaac gccgttgctc aggcgctcgg
     3901 gcagcatcgg gatgacgcga tcgacgaggt aaacgctgtt cccgcgatgc agcgcctcgc
     3961 gatcgagcgc gctgcccggg gtgacataag ccgagacgtc cgcgatgtgg acaccgagtt
     4021 cccattcgcc gcccgcgagt tgctcaacat tgatcgcgtc gtcgaaatcg cgcgcatcgt
     4081 cgggatcgat cgtgacgatg aattttccgc gcaggtcctc gcgctccccg aagcgagccg
     4141 gatcgaccgt tcccgaaatc ttattcgcct ccgccaggac agcggccgga aactcgagcg
     4201 cgaggtcatt cttgcgaatg atcgagagca tgtcgacccc ggggtcggtg gcgcgaccga
     4261 gcagctcgat gatttcgccc tcgggattga cgtggcgcga ctcccaggct tccagcctaa
     4321 cgacgacttt gtcctccggc gcgggcgacc ggggcgcgcc ggggagcggg cggggctgca
     4381 catagacgtt gtgcacgagg cgcggatcgt ccggcacgac gtagaaaaag tttcgcgttt
     4441 gctgcaaggt gccgacgatc gtgtcgtggg cacgctccag gatgcggatg acacgacctt
     4501 cggggcggtt gctcccgctg cgcgcgcgcg cataatcggc atcgcgcgta atccgcgcga
     4561 cgacccggtc tccgttcatc gcggtgccgg tgttctccgc ggcaataaag atgtcgccct
     4621 cgtcggaacc ttcgcgggcg agaaatccaa agccggcctg gtggatttgc aggcggccgg
     4681 tcgcgagatc ggcttccgcg ggcaggatat agcgatgctt gcgaatgcgg gcgatctctc
     4741 cggcgcgctc caactcgcgc aacgcttcct tcacgccgtt tcgaccctcg ctcttgaagc
     4801 cgagcgcgcg ggctagctcg gcctcgtcga gcggccggta tttcggcgtg ccgaggaggg
     4861 cgaggattcg ttgcttggtg tcggcgacgc gtttcatgct gccctgcgat gatcgaagaa
     4921 accgcgctga ttgaaagcag acatttgcga cggctcgcga cgcgccaacc tggaccgcgc
     4981 ccccaggttt ctatctaatc cgcggccggt cgaataaaac gacggctgtg ttagtctgaa
     5041 acaatcgatg gaggcgacgc gctggcagat aggcgacaaa gtcctcgacc tgacggctcg
     5101 agcgaggttg atgggcgtgc tcaacgtgac tcccgattca ttctccgacg gcggggaatt
     5161 tttcacgacg gaagcggcgg tcaaacacgg tcgcgagatg gcggaccacg gcgcggagat
     5221 gatcgatgtg ggcggcgagt cgacgcggcc cggcgcgcgg ccagttgcgc ctgacgagga
     5281 gatggagcgg gtgctcccgg tcatccggca gctcgcatca tccgttgcgg ccgccatctc
     5341 aatcgatacc tcgaaggcgg tggtggcgcg ggccgcgctt gccgccggcg ccacgatcat
     5401 caatgacgtg accggtgggc agcgcgaccc ggaaatgttc gcgctcgccg cggaacggcg
     5461 cgcggccctc atcatcatgc acatgcaggg caccccggag acgatgcagc tcgacccgac
     5521 ctatggggat gtcgtcgacg aggtggccga tttttttcga cagcaatttg cgcaggccgt
     5581 acgctgcgga gtaaatccca tgtgcatcgc gttcgatccc ggcatcggct tcggcaagac
     5641 agtcgcgcac aacctggaat tgctcgctaa tctcccgcgc ttgcaggtgg aaaatcggcc
     5701 gctcgtggtc ggcgtttcgc gcaaatcctt cctcggaaaa atttccggcc tgccgagcga
     5761 ccgggcgagc gcgacggtgg ccatgacttc gctgctgcgc gcgcgcggcg cacacgtttt
     5821 gcgggtccac gacgtgctta caaatgccag cgcgctacga acaacggaag ccttgttggc
     5881 cgccactcga tgatccacga attcaccctg ctttggttcg ataaatggcg gagcgccgtc
     5941 gaaatcctgc tcctgagtgt ggtgatctac tacggttatc tctactttcg cggcacgcgc
     6001 ggcgccaaag tattgaccgg tctggcgatc gtttttctca ccctgatcct gatctcgcag
     6061 ctcttgaacc tggtcgtgat cagcttcatc atccgcagct tttcggtctt cctcgcggtc
     6121 gcgctggtgg tgattttcca acccgaattg cgccgcgcac tggcagaact cggcggtcat
     6181 cccatctttt ccctgacgag cgagaagcgc gagacggtgc acgatctggc ggaggtggtg
     6241 acgcagcttt cgaacaagca gttcggagcg ctgatcgcga tcgagcgcga tacttcgatc
     6301 aagatctatg aagagaccgg cgtgatcgtc gatgcggcgt tctcgacgga attgctgctc
     6361 acgctttttc acccaaaatc cgcgttgcac gacggaggcg tgatcctgcg caacggtcgg
     6421 atcgctgccg cagcctgtat ttttccggtg agccaacgcg aaacgctcga tcgcagcatt
     6481 gggctacggc atcgggccgc gctcggcatt accgaagagt cggacgccgt cgcggtcgtc
     6541 gtctccgagg agacgggggc gatctcgatt tgtcaccgac gccggctcga gcgcaatttt
     6601 tcccccgaaa ccttccgaca gcggctcggc gaaatcttac tccatcgcac tcatgaagaa
     6661 actgatcctg aacaactggc gcgcgaagct gacgtccctc ctgctcgcga ccgcgctctg
     6721 gtacctcatc aagaagaacg taacaacgac accctcgccg tctgaggtcg ggagccggcc
     6781 ggaaacggcg gaggttcgtt aggcggtcct agcgcaaaaa gcgggcgagg ttctgatctt
     6841 cccagcggcg ggcgcgccgg tagcgcggga aagctctttc gcgccgataa aattcttcgg
     6901 tgtggacccg gcggcggccg gtcgcatccg tttcctccgg cacgactgaa tgcagcatct
     6961 cgtgataaag cacgtactcg agaaaccagc gcggaacgaa cggctgatcg agcaagggat
     7021 gaatccgaat gacgcgatcc tcctcctgga tcgagccaaa gatgaaatat tctttcggcc
     7081 gctctttccg tcgccgaccc cagagcactt tgtaaccacg cagtcgcccg cggaaatgac
     7141 ggtgattcac gcgatcgaag atgtggcgca gatcaaaatg gcgaccttcg tgccgaagat
     7201 tcaactgccg ctgcagcggc agcgtcggcc gggccagttt tcgtttcgtt ttctttgcca
     7261 tcggaaaccc tcgatccagc gggagaactg acagcaagtt tagtttttct ttagcttact
     7321 gacaaggttc agcgtttgtc agaatgaaga agcatatcgt aaatcgttgg cgtggaggtg
     7381 aataaaattt attttcgcca gcatttcgtc ggctactgga cttccgccgc ccctttgcga
     7441 tctttaacct ctgcgaacgg aaaatagccg gccaccagat gacaccattt cttcgtaaac
     7501 tactgggttt aaattggttg ctgattgtaa atatgctggc gttggccatt ttcggcgtgg
     7561 tcgcgatcta tagcgcgacc tacatgcgcc aggatccggc cgcggcggag ttttggcgca
     7621 aacaagcgaa ctgggtcgcc gtcggattct tcgctttcat cgtcacgagc ctgattgact
     7681 atcgctggat caaatggggc gcgctgccga tgtatctggc tggtcttttt tttctgatcc
     7741 tgacgcgttt catcgggacg aaggtttacg ggtcacgcag ttggctgcat ctcgggccga
     7801 ttaattttca gccagcccag ctcgccgtcg tcgccggcat catggtgctc gcgctttttc
     7861 tgagccagtt ccggcaaatg catccgatgc tgcgcctgac cctttgcgga gcgattgtgg
     7921 gcgcgccttg tctgctcatt ttgatgcaac ccgatctcgg cgaatgcatg atctgggggc
     7981 ccgtcctgct tgcgctgctt ttcgtcggcg gacttccgtt gcgctacttg atttgcgttg
     8041 tgctcattgc ggtggcgttt atcccgatcg cctatgtcga actaaaaccc taccagcagg
     8101 agcgcatcac cgcgttcatc aaccccgata tcgatcgaca gggctcggcg tgggcgatca
     8161 atcaatcgct catcgccatc ggctccggcg gctggtcggg gaagggattc aaagcgccga
     8221 atacgcagat cgagctcggt tttctccccg cgactgcggt gcataacgac tacatctttt
     8281 ccgccatcgg cgagcagtgg ggttttgtcg gcggcatcgc cctgctcggc gcctttggcc
     8341 tgatgttgct gacctgtctg ttcgtggcct acaaagcgag cgaccttttt gggctcttgt
     8401 tggtcattgg catcagtacc ctgattttca cccacatctt tcagaacgtc ggaatgacga
     8461 tctccatcct gccgatcacc ggcgtgccat tgccgctcat cagctacagt ggttccttcg
     8521 cgctcatgat catgttcggt cttgggatcg tgaacagcgt ctgggtgcat cgcaaagtgc
     8581 cggtgcgctg atttgctgaa catgccttcg ccgtggacaa atccgcggct ttggccatat
     8641 tgcccgtccc gatgtccatt ctgatcgact ccctgcttgt gttcgaggcg ctcgtcgccc
     8701 tgctcatgat gctcttcatc ctgatgcagc gaccgaaaag cgagggactc ggcgcggcct
     8761 tcggcggtgg cgtgacggaa aacatcttcg gagcgcagac cacgaatgtg ctcaccacca
     8821 tcaccacgtg gctggcgagc atctttttca tcgtcgtgct cgcgctgtcg gtgctctacg
     8881 cgcaccgcgg ggcacccgat tcggccgtgc ggcgggaatt gatgaaaaca tcggccgcag
     8941 cgaaggcggc cgcgccgctt ccggccaagg ctgcggccaa gaccgccgcc gccgatgcca
     9001 aacagaaagt ggctccggaa gcgaaagcga gcgtcccgcc agctaagacc gcgccttctc
     9061 cggccgcgtc cgcgcagccg aaaccctagt cgccgccagc tcgctccgcc gcgcgattct
     9121 tgtaatcgca gcggcccgac gaatgttgag acgcggagat gacatcgact cagcaacgga
     9181 cactggcctc tgcgaattgc gaaatcgcct tcgatgatcg cactcgccgg ctctacgcga
     9241 ccgacgcctc actttatcag atcgagcccg ccgccgtcgc cttcccgcgc agcgcagagc
     9301 aggcccgcga cgtcattctc gcctctggca gcgccggcat ttccgtctcg ccacgcggcg
     9361 cggggagcgg cctttccggc ggcgcgctcg gcgatggcct gatcgtcgat ttttcgcgcc
     9421 acaaccggca gatcagcgcg ctgaatctcg agcaacggac tgtccgcgtc ggcgcaggcg
     9481 tcgttctgga tcaactgaac gccttcctcg agccccacgg attttgtttc ggcccggacg
     9541 ttgcgagcag ctcgcgcgcg accttgggcg gaatgatcgc gaataactcc tccggggcac
     9601 atgtcccggt atacggcacg acggcggatc acgtgcgcgc ccttgaaatt gtgctcgccg
     9661 acggtcgcac cgagacgatc ggggccgatc gtccggcgct cgccgcggag caggaaatga
     9721 ttgcgcgcct tgtccgcgcg caaagcgccg agattgcgga gcgcatgcca ccgggaatgt
     9781 tgaagcggtg gtgcggctac gccctcgagc gttttgcgcg cgcccccgcc aacctgatcg
     9841 agattttatc cggcagcgag ggaacattgg ccgcgatcct gtccggcgaa ctgcggatcg
     9901 ttccgctgcc gcgcgccaaa gggttgggcc tgattttctt cgcttcggtg ggggaagcga
     9961 tgcacgcgac ggtcgagctg ctggatctca gaccggcggc gatcgaacac atggaccggc
    10021 cgctcttcga gcaaacgaaa ggacagcgga acttcgcagc ggcgagaagc ttgctcgagc
    10081 tcgacaccaa gccgtgcgag gcgatcctgg tcgtcgaatt tttcgacgac gtggcggatc
    10141 ggctcgccgc ggtcgcggcg cggaagcttg gattgcgcac gctgatttgt gcggatgaag
    10201 cggagatgaa tctcgtctgg tcgatgcgca aggcgggtct ctccctcctc acgggaagac
    10261 ccggagcggc caaaccggtc gcgttcatgg aggacgcggc cgtgcgcccg gcgcagttgc
    10321 cggcctatgt cgccgggctg gaagcgatca tgcagccact tggtttggat gtctgctatt
    10381 acggccatgc cgcggcggga ttgctccacg tgcggccggt gctcgatctg cacaccgcgg
    10441 ccgacttgaa aaaatatcgg caggtggcgg acgaagtagc ggccctcgtt aggcaattca
    10501 aaggctcgct ctccgccgag catggcgtcg gaatggcgcg caccgaatat ctacgcgggc
    10561 aggtcgggcc cgagttgttc gaggtcatgc gcgccatcaa acgtgttttc gatccggcga
    10621 atctttttaa tccggaaaag ttgatcggcg acggtcgctt ccggatcgac caggacctgc
    10681 gggagaattt tcgccgcccg atcaagctgc ccttcactcc ggtgctggcg tttgcggcca
    10741 aggaccattc cttcattggc aacctcgagc aatgcaacgg ttgtggcggc tgccgcaagg
    10801 aaacgcccac catgtgtccc actttcctcg ccaccggcga ggaatacatg tcgacgcgcg
    10861 gccgagcgaa tgcgattcgg gcggcgctcg atctgcgagt cacgggcgga gacccattgc
    10921 gcgcccaaat cttggaagac gcgctgagca attgtctctc ctgtaaagca tgcaccaccg
    10981 aatgcccctc gaatgtaaac ctcgccttgc tcaaggcgga actgatccac gcgcgccacg
    11041 aacgcgatgg cctgccctgg cagacgcgca ttttaagcgc cgtcgatttg ttaggccggc
    11101 tcggctgcac cctgccggcg ctggccaacg cggctctcga ttctccgccc ctccgggcag
    11161 agatggagaa aacgctcggg ctcgcggcgc aacgatcact gccacactac gccgaggagc
    11221 gcttcgatcg ttggttcgtc aggcacgcgc gcccgacaga cggaaagccc cgcggccgag
    11281 tgatcatttg ggacgacacc ttcatgcgct atcacgagcc gcacattggg attgcggcgg
    11341 tcaaagtgct cgaagcgctg ggttttgagg tcacgctccc gaccggtcgc cggtgctgtg
    11401 ggcgacccgc gtttagcctg gggaacttgc aggaagccgt ccggctcggc cgacataatc
    11461 tccatctgct ccagcgctcg ggcgagacga cgccaatact ttttctcgag ccctcgtgtt
    11521 actcgatgtt cgttgaagac tatcgcgaat tgaacttgcc ggaaacggag gcggtcgcgc
    11581 gccgctgctt tcttttcgag aaatttgtcg acgacctgtt ggagcgcgag ccggacgcaa
    11641 tccggttcgc gcagcgcgcg gtcccggttg cgattcacgc gcattgtcac gcgaagtcac
    11701 tgcttgatcc cggattcatg gtgcgcctcg cggaacggct cccgggccgg aaggcgacct
    11761 tgctcgagac cggctgctgc ggaatggcgg gcgctttcgg aatgttgaaa gcgaagtacg
    11821 atctttcgct caaggtggcc gcgccactgg tcgagctggt catcgatcaa ccagtcgatg
    11881 cggtcgtcgt cgcctccggc acgagttgcc gccaccagat cgacgatctc acgccggcgc
    11941 gtccgcgaca tatggcggag ctgctggcag acgcgatcgc tcgttaggcg aaatctgcct
    12001 tacggtttcg gcggagccgc cggtgggctg gcaccgggct tggccgctgt gcctggcgcc
    12061 ggtggcgcgc caccgggcag atccgcatcg acagggaccg ccagaatgtc gggttcggtc
    12121 cacgattcgg tgcgaccggc gagttctttg cgcaaggcac caactccctc cggcgcgatc
    12181 ggtccgccgg tgttgcccca ttcctgccga acgtaggtca gcacctcagc gattttctgg
    12241 tcgttcagag ttttctccca aggctgcatc acggccgtgc cgaaatgttg acccttgatt
    12301 gtgagcggac cctgcaaacc cttgagcaca atcatcaccg gccggcgccc acctccgttg
    12361 acgatctccg agccggccag cggcggatat tgccccggca ccccgagccc gtttgcctgg
    12421 tgacaggtgg cgcaattggc ggagaaaatc ttttggcctt tctggaccgg cgttagttcg
    12481 gccacctgct cggcgccccc cgcggcaccc gccttggtcg caacgcgcgg cgcgccgccc
    12541 aggtaatcga gcccgtcgcc gctaaaattt ccggcatagc gcccgaggta ggcaccgccg
    12601 aaaaagaccg ccaaaccata gatggtgatc agccagaagg agagcggctc gtggccgacc
    12661 cgcggctcgc ttttttccct aacgatcgcc gcgtgcgtgt cgtggatgcg ctgcgtttcg
    12721 ccgtaatcca ttccctggcg cggcaaattc tcaggcggct ggccggtcat ttctgcggct
    12781 ccttggcggg cgcaggcgcg atggtggccg gcgccttcgc ttccttcagg ggatgcgatt
    12841 ggtcgcgtga catcaggtaa gcaacgagac atttggcgtc gtagcttggg acgatttccc
    12901 aacctgccgc cggttcttcg tcaggatcgc cggcgaaatt aatggcgtcg ggcgaagcgc
    12961 tgccggcgat ccggcgtttc tcgtagagga accgaaaggg gggcatgttg gaatcctccg
    13021 agagactccg cgcattgtag aggtggcgat gatgccacgc cgcggtgtaa ggcaagggct
    13081 tgccgtccgc ggttagctca ctggcatttg caactaacga gggcgcgggc gttggagcgt
    13141 tggcaacggg gaccgcagcc ggcgccggcg cactggttcc ggcaggagat gctgctgcgg
    13201 cggcggcagc ggcgggagaa gcggtcgctg ccggcgcacc agctgcggat acagccgcac
    13261 cagtcgccgg agcagccgga gcagccgggg ctggggtcgc tcctttcgct tccggcatcg
    13321 accgatgacc gacattagcg agatcgggcc cggtgcgcat cttgccgaga aaaacgatcg
    13381 gctggaaaat gtaatcgcgc ggcgcactgc ggcggtcgcc ccatttgcgc tccagatcgg
    13441 caccgccgta atccggacgc acttgttgcg aatgacaata gacgcagcca ttggcggcgt
    13501 agacctgcgc gccgcgcagc gtcatgccgg agggcggcgc gggataaacg tcgccaccgt
    13561 cctcatccgt ttgtggacta agactgccga tctgcaggtt cggcaccact gtcatgccga
    13621 gccacgagaa ggcgaagatg ccgaaaatgc cgaggaagag tggctgtagt cccttcatca
    13681 gtgcccctcc cccggttcac gctcgagtgg attcaagaaa gtcgggacgc tcgcggtccg
    13741 gccaaagccg agcagcatca agccaaaatg gaaggcaaag acaaggtgcg aaaccgtcaa
    13801 caaaatccag gataaagtgc ggccgcgcag ataaggcaag accgtgtccg tgatttccga
    13861 gaaggggagc gtcggatcgt tcaggctgag gccctgggcg aatccgccga ccagaagcaa
    13921 gagcaccatc aagccgatgc cgtaggcgga accccagaag tgcaacttga tgagggaggc
    13981 ataacgccac tcgcgcccga cgagccgtgg cacgatgtag tacatcgagc caaaaatcac
    14041 catcgtaaag aaggcgtaga ggcccatgta ggtgtaggcc acgctcgctt ccgtaaactg
    14101 gacaatccgt gccatcgagc gcagggagat gatcacacca accacgctga aggcggtata
    14161 ggcgatcgca ccgaaaaccg taaaacgcag cgtcgggcta taacgcatca acggaaaata
    14221 gccgcgcatg gtcatgtgat gattgaggcc gaccgtagcc accgggatga tcgtgaggat
    14281 ggtagcggcg atgctcgcgg tgatcatcca cgccgggaac ggtccatcaa ccagacgctg
    14341 cattccggtc caactggaga agagcgcgaa ggtccagaat ccgatggcgg ccagataata
    14401 actgtgcacc ggccggccga tcactttcgg gatcatgtag taggccgtgc cgagcccgat
    14461 cgaaccgaac caaaggaaga ggagattgtt ggcaaaccac cagttaaccg ccgcctgcat
    14521 cacgccctgc accggaagaa ggaaaagcat gacctgcgcg gcggcgtaga gccatggaaa
    14581 ccagaggaac gcgcctaaca aataccattg cgtgatgtaa acgtgtccgc ggcgccggta
    14641 gcgaaacatg atcaatgccc aggtcatgat gagcagatag gcgacgaaca gggtgctcgc
    14701 ggacgaaccg ggaaactcga gccattcgta gcccgtactg tcaccgagca agattccgac
    14761 gattcccagc agcacgccga gattccaaaa ggccgcgccg ctgatgagga gaagcggatg
    14821 gcgcagggtg gtccgacaga gccgcgccat caaccaaatc gaggttccca ttccggccat
    14881 cgatccccag ccgtaaatga tggcgttaaa gtgcgccgga ccaacccgcc cgagggtgag
    14941 aaagctcacg tcgccgagga agccaggcac atgcatcttg aacgaagtga gcacgccgaa
    15001 gaacgtggcg aggagcagcc aggcgatcgc cgaagcgtaa aaaacgagca ccggcaggcg
    15061 ggtcgaggca tcgatcagcg cgcgctcgac ccgctcgacg tcgctcgtcg ttacttccac
    15121 gtcgcctaac ggcggctcct gggtcaatgc ggcgggctgg ctcatgaatt ttcggattgg
    15181 gccgcggatt ccaacggccg cgaaatcttt cttcgctccc gacggccgaa ccagcgccgg
    15241 cgcggcatca ccaggtcggt ggcgcgaccg atcggttcgg cgcgatcaaa aatcacggcc
    15301 gcgttttcgt tgtagcgcct caggttggcg aaaaagtcgg agaagctcat cggtttcatg
    15361 gttgaccggg ccggggcaac ggcggcgttt cccccgcttt ggccgcgggc ggggcggcgg
    15421 caggtggtgt tcccggcgca gccgcgggcg cattcggcgc ggcggcgggc tccggcgtcg
    15481 cgatcggacc ggcgggcgcg ggttttttcg cctgcaggtc ggccagttcg agttgcatgg
    15541 cgcgctggat cgggatgtgg gcgacgcctt tgcctttgtc gacccaccca tagctgccca
    15601 acgtcttgtt ccagtcctcc cgcgccgtct tgagcttctc ggtgcgagtt ttcgcgcgat
    15661 cttcctcgta gctcgaaccg cggtgcatcg cgccgaaggt gatcgcgaca atcaccccaa
    15721 agaaaacgaa cagcaccacc gcccacaacc agctggaaag caggaagcgc ggctgctcgg
    15781 gatggatcag tgtttcgtcg ttaggcacgg ctagttaacg atgcggagcg attcgatcag
    15841 gcgcgggtca cggaccggaa agagcgaggt cttgccgatg atgcggaggt aaaaaaaggc
    15901 gagtgtgcca ccaatcccga cgagcggcag aaagtcgaga atgctgagat gaacgcccgt
    15961 cccgtggagc gcgggcagca cgatgacgta catgtcgagc gcctgcatgc agacgatcca
    16021 gcccgcgaca cagcagagca cgaacggctg ctttttgacc gagcgcagaa gcaagatggc
    16081 gaatggaacg aagaatcggc cgatgactaa gatccaactg agcacgttcc aagactcggt
    16141 attccgaata aggtaatact gggtttcttc ggggatattc gcataccaat agagcatgta
    16201 ctggctaaag ccgatgtagg cccagaagac cgagaacgag agcatccatt ttcccatgat
    16261 gtggtagtgc tcgagggtga cgacaccctc caggtaaccc gcctgccgca gcgcggtgat
    16321 cacaagcacg agcagcgcca tcgagctgcc cgcagtcccg gcaaaaatgt aagggcccca
    16381 catggtcgag aaccagtgcc agttcagact catcagccag tcaaaggcgc caaaggtcag
    16441 acaaaaagca aagagcggca gaccgacgaa agcggccttc cgcatcagga tggtgaagcg
    16501 cgggttgccg tcgcgatcct ggcggacgga ataacttcgg agcaggtaag ccacgatcgt
    16561 gagcgcgacg aagaagaaaa tcgcgcggcc gagaaagaac tcccagttga gatagccgcg
    16621 cttggcatcg agcaccgggt cgaccccgcg cggcacgtcc atccaacggt agagatattt
    16681 ccgcaagaac cagatcggca gaaaaaacac gcccagcagc gggaggagga gcgcgatgtt
    16741 ttcgagctgt cgcctaacga ccaccgacca ctccgcatcg accacgtggt gaacaatggt
    16801 ccaaaaaagg cagccgatgc agagggtgaa gaaaaagatg aagccgaaaa gccaggagaa
    16861 actgaattgc accggggaaa cgaaagcgcc gatgaaactg aggacgaggc caatgaggcc
    16921 gatcaccccg caaagagccg agagcccggc aaaacggccg ttttcaaaat actcgccttc
    16981 cggcgcgggc aggacgtggg atcgatcgct cattttttct ccggctccgc tttcttttcc
    17041 aattgcgcct gatcctcggg cggcacatcg gccgcgctgg cgtgctggct tcgctgcagg
    17101 gcccgcaggt aagagatgat cgcccaacga tcctgcaccg tgacccgcgg cccgtaggcc
    17161 atcatggtgt tcttgccgtg cgtgatggtg ttgaaaattt ccccgtccgc catcttgcgg
    17221 atgcggtcgt cctgcagact aacgaccgtc gccaggccat actgcttcac gattccgtcg
    17281 ccggccgcga gcgggccgtg gcacaccgcg cagttaatgt taaaacgctg ctggccgcgt
    17341 tgcatcaggg ccggcgtcac ttccagcgga atgccggtgc cccagttctc acccatcttg
    17401 ccggtgttat agtaatccgt cccctcgcta aacccaaggt gcggatgcat cgccatctcg
    17461 ccggcggcat cgaccgacgc gccgccgtcg tccggcagcg gcatgtcgta gccgatgggc
    17521 accgtaccct cgaccggctg gcgcgcgccg cgttgatcgg caaaaaaatc gagctccgac
    17581 tgcgcgcgca ccttcggttg gcgaaccatg tcggggaaaa tctcaatctc cggcttgccg
    17641 cttttttgcc cgcggaaacc gaagaccgcg atccaggcga tcgtcaccag aatgaaaatg
    17701 aggaagaagg cgcgcagcat gattactcct cgtggaccac ggtgatgtgc tggccgccgg
    17761 ttcgttccag cagctcgcgc gcttcgttct cgctgaaacg cggatcgcgc gcctcgagca
    17821 cgaggaaaaa tccgtcattc gtcacccgct tgaaccgctc ccagttgaac atcggatggt
    17881 tccagcgcgg gaggaaattc atcgcctgcc aggcaaaaaa cgccgcgaac gcggaaaaca
    17941 aaaccgtgag ttcaaacatg atcggaaaaa atgcgggcac cgtccaaaag ttaaagggtt
    18001 tcccgtgcac gattaacgga taaaggtatg acgatggccc gaaggtaagg acgcacgcgg
    18061 tcagaagtcc cgtcagcccg ccacacagca cgaccgcgct caaccacgat ttgccgagcc
    18121 ccatcgcgtc gtccatgccg tgaatcggaa agggcgaatg gacatcccag cgtttgtagc
    18181 ccgcgtcgcg cacttgcttg gctgcttcgt aaagcgacgc agcgctcgga tattccgccg
    18241 ccattccgta aacgcgatta ccgctcggcg taatcacgcg tgctcctttc ctccagcggc
    18301 gacggcatgt tgcccgtccg gcggcacgta gtgcggatca gcttccggga gcacgatctt
    18361 tacttccgac atcgcgatca tcggagcgaa acgaatgaaa agcaggaaga gcgaaaggaa
    18421 aatcccgaag gtgccgatga aggtccagat atcgacccag gtgggagtaa aaaagcccca
    18481 cgccgaagga agaaagtcgc ggtgcaaccc gccgacgatg ataatgaaac gctcgaacca
    18541 catcccggcg ttgacgcaca tgcagacgaa gtaaacgacg aagatgttgc gccgacacca
    18601 ccggaaccag aaaagctgcg gcgaaagcgt gttgcagaac aacatgccga cccaccagta
    18661 ccaccaataa gggccgaaga tgcggttata gaaggcgaat ttttcgtact gatttccgcc
    18721 gtaccaggcg atgaaaaact ccatcgcata ggcgtagccg acgatcgagc cggtgagcag
    18781 aatgatcttc gccatcttgt cgatgtgttg cggcgtgatg atgtcctgca gcttgaaaat
    18841 ggcgcgcgcc ggaatcatca aggtcaacac catggcaaaa ccaccgaaaa tcgcgccagc
    18901 aacgaaatag ggcgggaaga tcgtcgtgtg ccaggtcggg atgacggagg tcgcgaaatc
    18961 gaacgaaacc acgctgtgca ccgagagcac gagcggcgtg gaaagaccgg cgaggagcag
    19021 atacgccatc tcgtaatgcc gccagtggcg gttcgaacca cgccaaccca gcgcgaaaat
    19081 tccgtagaag aattttttga tcttcgaggt cgctcgatcg cgcaacgtgc caaggtcggg
    19141 aaccaatccg gtgtaccaaa aaaggaccga gaccgtaaaa taggttgaga ccgcgaaaac
    19201 gtcccagagg agcgggctac gaaagttagg ccaaatcccg tactgggtcg gcaccggcgc
    19261 gaggaaccac gccatccaga cgcggccgac gtgaatgccc gggaaaatcg cggcgcaaat
    19321 gacggcgaaa agtgtcatcg cttcggccga gcgattgatc gaggttcgcc atttttggcg
    19381 aagcaaaaac aggatcgcgg aaatcagagt gccggcatga ccgataccga tccagaagac
    19441 gaaattggtg atgtcccaag cccagccgac cggagaattc aatccccagg tcccgacgcc
    19501 ggtcgagatt tggtagccga tgcaggttaa tccaaaagtc gcgatcgccg aagtgatggc
    19561 aaaggcgacc caccaccagc gcggcgtcgg attctcgacc gcgccggaaa tgcggttggt
    19621 gacccaatcg aaattgcgct tgttcaggac gagcggaacg cgcgccagcg gcgacgccac
    19681 cgaagcggag gcctgcggtg cggtgattgg accctccatg ctagaattcg cctcccaacg
    19741 atgtcatcac ggttgcggct ccggcggcag ccccggtgcg gtattctcaa cgggcggctt
    19801 ggcttcattc ccgaggctgg cgacggcgat cacgccggca tccggcatct tcggattggg
    19861 attgcgaatg cgcgccaggt aactcgtccg cgtcttcacg tttaaatatt cgagcagccg
    19921 atagtttcgg ttctgcgctt tgatcttcga gactcgcgtc tccggatccg cgatatcgcc
    19981 gaaaacaatc gcctctgccg ggcacgcctg cgcgcaggcg gtggtgaagg aatcgcgcgg
    20041 aatgagcgtc ttgtccgacg cgcccgcgcg cacgtgggca gcgatcttcg cctcttcgat
    20101 tctttgcacg caataggtac atttctccat cacgccgcgc atccgcaccg tgacgttggg
    20161 atttttctgc agcttgatcg tatcgggcgc gcctttgtcg gtcaacggcc cgaggtattc
    20221 ctgataaacg gagaagccgc cgatcttctt tttgccgatc ggccgctgag tgtaatcgaa
    20281 gaaattgaag cgccgcactt tgaacgggca gttgttcatg caatagcgcg tgccgatgca
    20341 gcggttgtag gccatgacgt tgaggccgtc ctcactatgc acggtggcgt tgaccggaca
    20401 gaccgtttcg cagggcgcgt tctcgcagtg ttggcacatg atcggctcat gcaccatctc
    20461 gggattttcc ggccactcgc ccttatcctg atcgaaaggc ttggcgctcg cgtaatagcg
    20521 atcgatccgg atccagtgca tcgcgcgccc atgagagacc tgcaatttgc cgacgatcgg
    20581 gatgttgttt tccgcctggc aggcgatgac gcaagcagtg cagccggtgc aggtgttgag
    20641 atcgaccgtc atgccccact gctgcggcgc gtcgagtttc ggatgtgtgt aaagcgaggg
    20701 caattgcggc ggcagttcct cgtcacccgc gatcttcttc acgaaatcct cgtcaccgcg
    20761 ataatgttcg agagtcgctt cccgcacgag cccacggcct tcgatcgaga aatgctgctg
    20821 ggtggtggcg agcggatagg tgccggccac ctttttcacc tgcacgctcg cgatcgcctt
    20881 gccgtcggcg atgatgaaat gcgggttgga actggtgcgg agcagataag cgttaaagcc
    20941 cgcttcttcg ccgaccggac cggtgtgttt gcgtccgtag cctaacggaa tcgtgaccga
    21001 gttttccgcg tgtcccggga caatgagggc ggcgatcacc agctcgcgcc ggatcggttt
    21061 gccctggcga tccttcgccg tgtcggtgac cacgatttgc accaggtcgc catccttgac
    21121 gccggtgcga tgcgcaaaag ccggactcat catggcggcg ttgtcccagg tcagtttggt
    21181 gatcgggtcg ggcatttcct ggagccagcc gttgttgatg taacggccgt cgtctatcgc
    21241 gtaactgccc accagcacga tctcgggcga atctttcgtc ggcgccggcg cgtcgttgct
    21301 ccagagggtg tgcgcgacgc cgccggctga gttgccgttg aattttggcg ttcgatcctt
    21361 cggcggcagg tgcgcggcaa aaccgtcgtg caaaaacttg ctccacgccg tctcgaaatc
    21421 gcccggcggc gaggtcgcgc ggaaggtttc cttgacgagt tgcgggccat ccaccctcgg
    21481 ctgaccgagc accgtccaaa ggagatcgag ttccgacatc ccgccgaaga gcggcaggat
    21541 catgggctgg atcgcgacgt aggagccgct cggcgtgagg gcgtcgcccc aggtctcgag
    21601 atagtgcgcc gccggcaggt gccattggct caaagccgag gtggcatcct cgtactgacc
    21661 aagcctaacg acatcgggca ctcgcttctg caactcgccc cagtccaacg gctgcttggt
    21721 tttacggtcg agggtgatgg agcgcagagc attgtaaacc gggtcgccgc ccacgatgaa
    21781 gagctgctgg atacggccgg catcgatctc gctcgcgagt tgcaggatgc tgctttcgcg
    21841 cgacgtgcgc ggcaactcgc ggaggagcaa ggttgtgccg agattcttca gcgcggcgtt
    21901 gatcccatag accaggagtt gcaccacaac cggttgatgc ggccccgcaa caaccaggct
    21961 ggcgctcggt ttcgagagta aatcctgcgc cgcttcctcg agccagcgcc cgttgtattt
    22021 ctccgctttg gtcggcgcct tcagggtgat gagaaccgaa gagaggcccg gatccctggt
    22081 cgccaccgcg atcttgctcg cgagcgcgtg cgtgatcgcc gggatctggc tcgccccata
    22141 acgcaggcga tggtccgcca tcgcgccagt cagagtgaag cgattctcca ccacgtagag
    22201 ccggttcatc gaatccttgg cctcgcgcac gcggcgccgc gcagtgaaac cgcggatcga
    22261 agcgagatcg ccttcgccgc agtttagaaa atcgctgtcg agcgcgaaga cgacatcggc
    22321 tttgtcgaac tgcgggatca gccgcgtatt gtccccaaaa ctgatctgag tcgagaaatg
    22381 ttgcgcttcg ctgaggagcg ggtcgtagac gcaccagcgc atcttcggaa attgcttcgc
    22441 gagctcgttc cgcaaacgct cgcgcgtggg cgagtaggtt tcctcgacca gaaaggcgag
    22501 cccggcgcca cggttcgccg ccatcttcgg tcgcagttct gcgatgtatt tctcgaacgc
    22561 cgcgcgatcg gacaactcgt tcttgtgcac gaaacgcttc gagcgcgccg gatcgtagag
    22621 atcgagcacc gacgcttgcg cgaaggcatc ggtcgcgccg gcgctgtcga ggtgcagcgg
    22681 gttgccttca attttgatcg gccgaccgtc caccgtcgac acgacgagcg ggatcgcgcc
    22741 atggcgccgc ggcatggcgg tcgcgtagta aagcgccttg cccggaatga cccactcggc
    22801 gctcttcgtg aagggcacca aatgcatctc cggcttgcga cagctgttca acccgaaacc
    22861 ggccagcgcc atcgaggcgc ccatcagctt gaggaaactg cggcgtgaaa cttcgtcgcc
    22921 ttcgatctcc gccgcgccgg cgggaaattc ccgttccagc cagccgcgaa attccggcgt
    22981 gtcgctcagt tcgccgaggc tgcgccagta ttgcttcccg gtcttcgacg gggcgggatg
    23041 ttggagcacg cgcttcatcg gtggcacccc tggcaattga gcggcggctt gatgtcccac
    23101 cgttccttta aggtgagacc gatctcctcc tgcgtgagat tcgtcttctt cgaccagtct
    23161 tccttcatgc cctccggctg gccatatttc gcaacgaact ccgcgggctt cacgtccgaa
    23221 ggtttccaat ccaaatttgt gatctgatcc tccgggcgca ggaaattctc cggcgcgcga
    23281 tggcactcga ggcaccactt catgctgagc ggcttcgcat cgtagacgac cggcatgtgg
    23341 ttgatctcac catgacaact gaagcagctg atcccgcgat tcacatgcgc cgagtgattg
    23401 taatagacgt aatcgggagt gcgatgaatc tgcacccacg gcactggctg gcccgtcgtc
    23461 caactcgcgc gcaccggctc gagcttcggg ttttcttttt gcacctgggt gtggcagttc
    23521 atgcagactt gggtgtcggg tacgttggag tgggccgtga gctcgaccgc attgtggcag
    23581 taacggcaat ccattcccag ctgggtgacg tgcaggtcgt gcgggaacgg gaccggctgg
    23641 gtgggcatgt aaccgacgcg ggtatatttc ggcgtgtcgt aataccaggt cgcgccgacc
    23701 agcgcggtca cgatcacagt cccgcagatc atgatcttga gggggagcca gttggtccag
    23761 cgcgggaaaa aattggccat accgaattag cggagattag ccggtctcgg gagaggctgc
    23821 caactggtaa acgtgagagt tgaaatgaaa gggcgtagcg gcttcagaaa atgaaaaatg
    23881 agatttgtga aatgcgatcg ccggaggtga gtgggcaatt ctatgaacgg tcctttgctt
    23941 ggcaaaagaa ttttggaaaa gaagttactt ttctgcgcgg ccagcctttc cgtttcttgc
    24001 gcgcgggcct caatttctcg cctggcgccg tgccgcggcc tcgctcagtt cgggtgaccg
    24061 gcgacgggaa tcctggagcg attcgcttcg aggaaggaac tgatcttcgc tccgtcgaaa
    24121 atcggtgcgg agcagcgccg gtccgtgcaa atgaacgctg cggcttgttc gaacgtcggg
    24181 tattccacgt cggaatttgg gagcggccct tccgccgggt cccaccactc cacgcgtttg
    24241 taggggatcg gctgacgaac ggcggtcgcg aaaaggtcgc gtgccttcgg gtcgtgttta
    24301 ctcccgacca ccgtcagatg gaacggcggc gcgcgcagtt cacgatcagc caggagaatg
    24361 ccgccgacca aaaatccacg acgctccgcc accatcggca ctgccagata acgcatcgcg
    24421 tgttcggccc gcgcgtggtt ggcggcgcgg ccgccgcagt aagtcagctg attcgttagg
    24481 cgagcgaagg cggtgtt
//
LOCUS       DATMNS010000091         4672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1144184, whole genome shotgun sequence.
ACCESSION   DATMNS010000091 DATMNS010000000
VERSION     DATMNS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144184"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VNW28_03415"
     CDS             complement(<1..260)
                     /locus_tag="VNW28_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09004.1"
                     /translation="MVKLKDIEGTAALDLGGGGTPPPRPPLKAFDSTIRMASFLASLL
                     FAALCASPASAQIPRGIFSLGGSGNKASASALADPDVVGITIR"
     gene            434..580
                     /locus_tag="VNW28_03420"
     CDS             434..580
                     /locus_tag="VNW28_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09005.1"
                     /translation="MNFTKNIGMLLLAVYLIVVGIIGLTGMGVGILLPIVALIAGIFI
                     LIGK"
     gene            706..1089
                     /locus_tag="VNW28_03425"
     CDS             706..1089
                     /locus_tag="VNW28_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09006.1"
                     /translation="MKILTILARTLLGLIFVVFGFNGFLHFIPMPPPPSGSPAALFFT
                     AVSTTGYMKVVFLFQIIGGLLLLLNLLPVLGLVILCPIIFNIVLFHLTMAPSGLPLAS
                     VITVLALFLIGMYWDHYRHLVRQPR"
     gene            1086..1943
                     /locus_tag="VNW28_03430"
     CDS             1086..1943
                     /locus_tag="VNW28_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HXA09007.1"
                     /translation="MILCHDPRCAEYSAAGHPESPARVRWSASHLRQAHPDWEWRLPN
                     EASEAVLLRAQGAAHVERVRAASHDFDADTPAYANIFAHAARASGAACAVAQASLGGA
                     PAFSLMRPPGHHATRNQAMGFCFFNHIAVAALDALAHGAERVAIWDFDAHHGNGTEAI
                     VTGNPRIAFASVHQFPGYPGTGAHSSGNIHNFPVAPFAPRGEHCAAVARALTVLLEFK
                     PALLLVSAGFDAFVGDPITQMSLEIDDFALFGKWLRESSVPAGAILEGGYSDDLPQLI
                     DAFLCAWAA"
     gene            complement(1950..3290)
                     /gene="nhaA"
                     /locus_tag="VNW28_03435"
     CDS             complement(1950..3290)
                     /gene="nhaA"
                     /locus_tag="VNW28_03435"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HXA09008.1"
                     /translation="MNKLQDKLPLPGETPIERLIKPFEEFARLESSGGILLISCTVVA
                     LLWANSPWAATYFSLWHTKLTLGFAGAQLSEELHFWINDGLMAVFFLQVGLEIKREAL
                     VGELATARKAAFPLIAALGGMFVPAGFYFLFNRAGPGAAGWAVPMATDIAFALGVLAL
                     LGDRVPTSLKVFLAALAIADDIGAVLVIAFFYTAQISWMSLAVAGIFFVALLVMNRIG
                     ARHPLIYIVLGFGLWLAFLQSGIHATVAGVLLALTIPAQRRIDSGAFLERSEKILDEF
                     RSAQEGGDAIQASATRSAAFSLLAEDCHYAQAPMLRFEHALAPWIKHAVMPIFALANA
                     GVALGGGAIGALVGPISLGVITGLALGKPIGIVLFSWFAVRSRVATLPEGVCWRQIIG
                     VGVLSGIGFTMSLFIANLAFGGASELETAKVGILAASVASGIAGALVLFKKTRA"
     gene            3536..3609
                     /locus_tag="VNW28_03440"
     tRNA            3536..3609
                     /locus_tag="VNW28_03440"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3570..3572,aa:Pro,seq:ggg)
     gene            3772..>4672
                     /gene="ychF"
                     /locus_tag="VNW28_03445"
     CDS             3772..>4672
                     /gene="ychF"
                     /locus_tag="VNW28_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXA09009.1"
                     /translation="MLSAGIVGLPNVGKSTLFNAVTRTRKAQAANYPFCTIDPNLGVV
                     TVPDARLDVLSKLSHSQKIIPTAIEFVDIAGLVKGASTGEGLGNQFLSHIREVNAIVQ
                     VVRCFERGDIHHVSGTIDPVRDIEVINTELILADLASLQKRRDRLQKEIRAGSKTAKA
                     EAALIEKLLPHLDSGKPAITLTLSDEEKITVREFFLLTSKPTLFACNVGESDLAAIVE
                     KDEANAALRHVRAVEEYARNHFATEAVVISAQIESELVDLSEAEAGEYLGDLGVKESG
                     VGALIRAVYHLLGLRTYLTTGEKE"
BASE COUNT          930 a         1484 c         1378 g          880 t
ORIGIN      
        1 cggatcgtga tcccgaccac gtcgggatcg gccagcgcac tcgcgcttgc tttgtttccc
       61 gatccgccaa gggaaaagat gccgcgcgga atctgcgcac ttgcggggga cgcgcacagg
      121 gcagcgaaaa gcagcgaggc aaggaaggac gccatccgga tcgtcgaatc aaaagccttg
      181 agcggagggc gcggtggcgg tgtgcctcct ccccccaagt cgagcgcggc agttccttcg
      241 atgtccttca gctttaccat aaataccgta attaatagac gttgataagc tggagtcgtg
      301 ccatttcagg gcacaggcgc actttgcgga tgtgagaacc gcaatttgcc gcgaacgctc
      361 gggggcatac ggcggccgga ggaagacccg attaagcgtg catagcgaat tggagccgtc
      421 cacgatggcc gccatgaact tcaccaaaaa tatcggaatg ctcctgctcg cagtctattt
      481 gatcgtcgtc ggcatcatcg gcttgaccgg catgggtgtc ggcatccttc ttcccatcgt
      541 cgcgctcatc gccgggattt ttatcctgat cggaaaatag gagcgcgtcc tcgccaacgg
      601 ggccaggcgt cggttcatcg gtcgagtgac cgatccccgt ttctaaacgc gaacggtcgc
      661 aggtttcgcg ttgtctttcc ggtcgcgcca gagcaggttc ggcccatgaa aatcctgacc
      721 atccttgcgc gcactctgct cggcctcatc tttgtcgtct tcggcttcaa tggattcttg
      781 cacttcattc ccatgccgcc accgccttcc ggtagccccg cagcgctctt ctttaccgcc
      841 gtttccacca ccggttacat gaaggtagtg ttcctgttcc agatcatcgg cgggctgctc
      901 cttctgctta acctcctccc ggtgctcgga ttggtgatcc tctgcccaat catctttaac
      961 atcgtcctct tccatctcac catggctccg agcgggctgc ccctcgccag cgtcatcacg
     1021 gtcctggcgc tcttcctgat tggaatgtac tgggaccatt accgtcatct tgtccggcag
     1081 ccgcggtgat tctctgccac gatccccgct gcgcggaata ctccgccgcc gggcatcccg
     1141 agagtccggc gcgggtcaga tggagcgcga gccacttgcg gcaggcgcac ccggattggg
     1201 aatggcgttt gcctaacgaa gcttcggagg cagtcctgct ccgcgcgcag ggagctgccc
     1261 acgtcgagcg ggtgcgcgcg gcgagccacg atttcgatgc cgacaccccg gcttacgcaa
     1321 acattttcgc ccacgcggcg cgcgcttccg gggcggcctg tgccgtcgct caggcgtcgt
     1381 taggcggggc acccgctttc agcctgatgc gcccgcccgg acatcacgcc acccgcaacc
     1441 aggcgatggg cttctgtttt ttcaatcata tcgccgtggc cgcgctcgac gcgctggcgc
     1501 atggcgcgga gcgggtggcg atctgggatt tcgacgcgca ccacggcaac ggcaccgaag
     1561 cgatcgtgac cggaaacccg cggatcgcct tcgcctcggt ccaccagttc cccgggtatc
     1621 ccggcaccgg cgcgcattcg tcaggcaaca tccacaattt cccggtcgcg ccttttgcgc
     1681 cgcggggcga gcattgcgcc gcggtcgcgc gcgccctgac ggtgcttctg gaattcaagc
     1741 ccgctctctt gctcgtctcg gccggcttcg acgctttcgt cggcgacccg atcacacaga
     1801 tgagtttgga gattgatgat ttcgcgctct ttggaaaatg gctccgtgaa agttccgtcc
     1861 cggccggcgc gatcctcgaa ggcggctaca gcgacgatct cccgcagctg attgatgcgt
     1921 tcctctgcgc ctgggcggct tgaagatcgt taggcccgag tcttcttgaa gagaaccaac
     1981 gcacccgcga tgcccgaggc gacactggcc gcgagaatcc cgaccttcgc cgtttcgagc
     2041 tcggacgccc cgccgaaagc aaggttcgcg atgaataacg acatcgtgaa cccaattcca
     2101 ctgagcactc cgactccgat gatctgacgc cagcaaacgc cctctggaag ggtggcgaca
     2161 cggctgcgaa cggcgaacca cgagaagagc acgatgccga tcggttttcc gagcgcgagc
     2221 ccggtgatca ccccaaggct gattggaccg acgagtgcgc cgattgcccc tccaccgagg
     2281 gccacacccg cattggcgag cgcgaagatc ggcatgacag cgtgtttgat ccagggcgcg
     2341 agcgcgtgct cgaaacgcaa catcggcgct tgcgcgtaat ggcaatcttc cgcgagcaaa
     2401 ctgaaggccg cgctgcgggt ggcgctggcc tggatcgcgt cccctccctc ctgcgcagaa
     2461 cggaattcat cgagaatctt ttcgcttcgc tcgagaaacg ccccgctatc gatgcggcgc
     2521 tgcgcgggaa tggtaagggc gagcaacacc cccgctacgg tcgcgtgaat gccagactgc
     2581 agaaaagcca gccacaagcc gaaaccaagg acaatataga tgagcgggtg tcgcgcgccg
     2641 atccgattca tgaccagcaa cgccacgaaa aatatcccgg caacggcgag gctcatccat
     2701 gagatttggg cggtgtagaa gaaagcaatc accaggacgg cgccgatgtc gtcggcgatg
     2761 gcgagcgcgg cgaggaaaac cttgagcgag gtcgggacgc gatcgccgag gagcgcgagc
     2821 actccgagcg cgaaagcgat atcggtcgcc atcggcacgg cccacccagc tgctcctggg
     2881 ccggcccgat taaaaagaaa gtagaaaccg gcgggcacga acatgccacc gagggcggcg
     2941 atcagcggga acgccgcctt gcgcgcggtg gccaattcgc caaccagcgc ctcgcgtttt
     3001 atctcgaggc cgacctggag aaagaagacc gccatcaacc cgtcgttgat ccagaagtgc
     3061 aactcctcgg agagttgcgc gccggcgaaa ccaagagtca gcttcgtgtg ccaaaggctg
     3121 aagtaggtcg ccgcccaggg tgaatttgcc cagagcaatg caaccaccgt gcagctgatg
     3181 aggagaatgc cgcccgaaga ttcgagcctc gcgaactcct cgaatggttt gatcaaccgt
     3241 tcgatcggcg tctcgcccgg cagaggcagt ttatcttgga gcttgttcat ttccgccgag
     3301 ctcgtcttgc ctttggccgg ctcacccatt gctttccgtc gcgccagtaa atttgtttgg
     3361 caagcgcaac cggtccggcg aatatttcgc ccttcggacc gtagcacaat ctgccacgag
     3421 gcggactcgc gggggcgagc ttggtagcgc ggtcgccgcg gcgacagatc gccacttcga
     3481 ctctcgccaa aatcatttgg caaaggtgac gcaagcagcg aataatccgg cacgtcgggc
     3541 cgtagcacag cttggtagtg cgcttgaatg gggttcaaga ggtcgcgggt tcgaatcccg
     3601 ccggcccgat tcttccgtca tcccgagcac ggtcgaggga tcccgcggcg tcgccttcaa
     3661 ggttccacct gggattgctc ggcagcgccg cgctccgctc gggatgacag aaaacgactg
     3721 taaatcctgt ctgattgcgg ctacggcgct ccggccttaa cctccgcgcc tatgttgagc
     3781 gccgggatcg tgggcctacc taacgttggc aaatccactc tgtttaacgc tgtcacgcgc
     3841 acccgcaaag cgcaggcggc gaattatccc ttctgcacga tcgatcccaa tctcggcgtc
     3901 gtgaccgtgc ccgatgcgcg cctcgacgtt ctttccaagc tttcgcattc gcagaaaatc
     3961 atcccgaccg cgatcgagtt cgtcgacata gccggactcg tgaaaggggc aagcacgggc
     4021 gaaggactcg gtaaccaatt tctcagccac atccgcgaag tgaacgcgat cgtgcaggtc
     4081 gtacgctgtt tcgagcgcgg cgatattcat cacgtcagcg gcacgatcga cccagtgcgc
     4141 gacatcgagg tgatcaacac cgaattgatc ctcgccgacc tcgcctcgct gcaaaaacgc
     4201 cgcgaccgac tgcaaaaaga aatccgcgcc ggatcgaaga cggcgaaagc cgaggcggcg
     4261 ttaatcgaaa aactcctgcc ccatctggat tccgggaaac cagcaatcac gctcacgctg
     4321 agcgacgaag aaaaaatcac ggtgcgtgaa tttttcctgc tcacctccaa gccaacgctc
     4381 ttcgcctgca acgttggcga atccgatctc gccgcgattg tagagaaaga cgaggcgaac
     4441 gccgcgctgc ggcatgtgcg ggcggtcgaa gaatacgcgc gcaatcattt cgcgactgaa
     4501 gccgtcgtca tcagcgcgca gattgagagc gagttggtcg atctctccga agccgaagca
     4561 ggcgaatacc tgggggatct cggcgtcaag gaaagcggcg tcggcgcact catccgcgca
     4621 gtctatcacc tgctcggatt gcgcacctat ctgacgaccg gagaaaagga aa
//
LOCUS       DATMNS010000092         5799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2930771, whole genome shotgun sequence.
ACCESSION   DATMNS010000092 DATMNS010000000
VERSION     DATMNS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5799
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2930771"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VNW28_03450"
     CDS             <1..351
                     /locus_tag="VNW28_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09010.1"
                     /translation="VRGEREPGSMTLPQILAGRAAGLLLRLLYRVSYTDMGPFRAIRR
                     DALARLGMREETYGWNLEMQMRAARAGLRILEVPVNHRCRAGGESKVSGTVRGTLVAG
                     ARIAATLLRVATGR"
     gene            complement(376..1620)
                     /locus_tag="VNW28_03455"
     CDS             complement(376..1620)
                     /locus_tag="VNW28_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09011.1"
                     /translation="MPKTTQSAAEVGWKLFPIALAVLVAVFFSWSVRPTQQHFDYTFR
                     IADAFLHGHLGLNAQPPSWLNEMVLVREKYYSVFPLGAVLAVLPFALLGEAGLVKGFF
                     GGSLAALIAGLCVYSFYQLAGVGPNSNSKPRRILLALFPIFGTWSWCNLGLGGSWQIA
                     LGFALLGQAGALYFTLHRRGPFLAGACFALAFGNRTELILTIPIYLYFWGPWSEAHLR
                     SANRASKGLPRGWWKPLGWFLAIPAALALGTAAYNFARFDSIFDFGYARIANLTQEPW
                     YRSGLFSFHAIPWNAFKMLFEGMQDIPTFPYLRPYPFGCSIFLSSPFLCLLFREGGDL
                     KALAWSVIGLLTLVLWCHGNPGGWQFSYRYAMILLPWMFLLLLRNGPRKLSATELGLF
                     LVSVAINAIAVYEFLWTNNIHP"
     gene            complement(1725..2519)
                     /locus_tag="VNW28_03460"
     CDS             complement(1725..2519)
                     /locus_tag="VNW28_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HXA09012.1"
                     /translation="MPELAEVEYFRKRWDAGLGEKIVAVHLHGEKRIFRGTDVRALGR
                     GLVRQTLLRSKTRGKQMLFEFSGDHWLGIHLGMSGKLRLETSKFHPARHDHLVLQQRK
                     WALVFSDPRQFGRIRLHHGKEPPSWWDVSVPEIHSRGFTKSWLREFVRRHARAPIKAV
                     LLLQKGFPGVGNWMADEILWRARIAPRTRAGKLSVERLGAIWRATRFVAQASLQTIGR
                     DFSDPPKSWLLHERWKAGGKCPRDGTTLRRATIGGRTTAWCPKCQR"
     gene            2549..3484
                     /gene="trxB"
                     /locus_tag="VNW28_03465"
     CDS             2549..3484
                     /gene="trxB"
                     /locus_tag="VNW28_03465"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HXA09013.1"
                     /translation="MLPLARMEKVIIVGSGCAGLTAAIYAARANLSPLVLEGRQPGGQ
                     LSTTTMVENFPGFPEGIDGPQLIMNMHRQAEKFGARFSYAEATDLEMTGDHLRLQADD
                     EWLETQALIIASGASARYLGLENEEKLIGHGLTSCATCDGAFYRNVPVCVVGGGDSAA
                     EEALFLTRFASKVYLIHRRDQLRASKIMAARVLAASKIEAIWNTVVTKYLADDKGEMT
                     AVELRDVVTKKEKILPVACVFVAIGHDPNTKAFAGKLETDPDGYLFVRHLAESKHPGI
                     FIAGDVADRVYKQAVTAAGSGCAAAIEAEKYLSGL"
     gene            3588..4760
                     /locus_tag="VNW28_03470"
     CDS             3588..4760
                     /locus_tag="VNW28_03470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA09014.1"
                     /translation="MKLCDLTQFYSPLSGGVKRYVHEKIRYIQRATDDEHVLIIPGPK
                     TELIEAERSRIYAIGSPLLSRTSRYRALLNLRAIEEILEREQPSLIESGDPYQVAWKA
                     IASGVGLRIPVVGFYHSHFPEAYLRSTARYLGRTATEFVMDLARRYVRKIYNQFEATL
                     VPSARLGQLLTAWGVENVRAVDLGVDVEIFHSQPEDGAVTRDSLGISAGCCLLLYVGR
                     LAQEKNTQTLFAAFELLAAREPQRYQLLIVGDGLQRAELRALQERTGTVTWRPYCTDS
                     EELARLYRAADLLVHPGVQETFGLTALESQACGTPVVGIRGSYMDRIIFGGQEHWAKE
                     NSVPALADAVAAAAGGELRKTGLVAAGRVREHYAWPVVFAQLFQIYREVRAGYHAD"
     gene            4837..5526
                     /locus_tag="VNW28_03475"
     CDS             4837..5526
                     /locus_tag="VNW28_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09015.1"
                     /translation="MKTDPAYTIRSYRAADRAAVRRLCCLTGFLGSPIDPVYEDRELF
                     ADFLTTYYTDHEPESSFVLEVDGELKGYLLGSRKPLLNQLYSFQQNIVLFLRSLLRYP
                     RYNVRSRRFLRWMMMNGWREVPAAPRRTPHFHINLLPEARQISTTRALMSAYLSYLYR
                     RGEKRVYGQIVTFESRRGEKMFERYGFRVMNRSEITKYRAFYPESVYLSTVIKNLETA
                     GPLSAYSDSAT"
     gene            complement(5564..>5799)
                     /locus_tag="VNW28_03480"
     CDS             complement(5564..>5799)
                     /locus_tag="VNW28_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09016.1"
                     /translation="SAGYRPQPYHAPVALLLSEDVLPENMLASDWQHLAPNIVIHPLR
                     GSHLECITTHVEILAEKIKTCLQMGAAHSVSQK"
BASE COUNT         1178 a         1702 c         1832 g         1087 t
ORIGIN      
        1 gtgcgcggcg agcgcgaacc cgggagcatg actttgccac aaatccttgc cggacgcgcg
       61 gccggattgc tcctgaggct tctttatcgc gtctcctaca ccgacatggg tcccttccgc
      121 gcgatccgcc gcgacgcgct cgcccggctg gggatgcgcg aggaaaccta tggttggaat
      181 ctcgagatgc agatgcgcgc cgcgcgcgcc gggctgcgga ttctcgaggt gccggtcaat
      241 catcggtgtc gcgcgggcgg cgaatcgaag gtctcgggaa ccgtgcgcgg cacgctggtc
      301 gccggtgcgc ggattgccgc gacgctcctc cgcgtggcaa ccggacggta agaagcccgt
      361 gcgcggggga gcagtttacg gatggatatt attcgtccag agaaattcgt agacggcgat
      421 ggcgttaatc gcgaccgaaa caagaaaaag acccaactcc gtggccgaca gctttcgcgg
      481 gccgttccgc aacagcaaaa ggaacatcca cggcaacagg atcatggcgt agcggtagga
      541 gaactgccag ccgccgggat tgccatggca ccacaggacg agggtcaaaa gtccaatcac
      601 gctccaagcc aaagccttga ggtctccgcc ttctcgaaaa agaagacaaa ggaatggact
      661 gctcagaaaa atggagcagc caaacgggta aggtcgaaga tacgggaagg tcgggatatc
      721 ctgcattccc tcgaacaaca tcttgaaagc gttccacgga atcgcatgga acgagaatag
      781 gccgctccgg taccacggct cctgagttag attcgcgatg cgagcgtaac cgaagtcgaa
      841 aatcgaatcg aagcgggcaa agttgtaggc cgccgtgccg agggcgaggg cggccgggat
      901 ggccagaaac cagcctaacg gtttccacca gccgcgcggt aaccctttgc tggcgcggtt
      961 cgccgatcgc aggtgggcct cagaccaggg accccaaaag taaagataaa tggggatggt
     1021 cagaatcagc tccgtgcgat tcccaaaggc caaagcaaag caggcgccgg cgagaaatgg
     1081 tcctcgccgg tgcaacgtaa aatagagcgc cccggcctga ccgaggagcg cgaatccgag
     1141 ggcgatctgc caggatccgc cgaggccgag attacaccac gaccaagttc caaagatcgg
     1201 gaacaaggcg agcaggattc ggcgtggttt tgagttcgag ttcgggccga caccggccag
     1261 ttggtaaaag gaatagacgc ataatccggc aatgagtgcg gccagggagc ctccgaagaa
     1321 tcctttcacg aggcccgctt cacccagcaa tgcgaatggc aaaacggcaa gaactgcgcc
     1381 gagaggaaag acggagtaat atttctcgcg taccaggacc atctcgttga gccaggaagg
     1441 cggctgcgcg ttcaggccca aatgcccgtg caggaatgcg tctgcgatcc gaaaagtgta
     1501 atcgaaatgc tgctgcgtcg gccggactga ccacgagaaa aaaacagcga ccagaaccgc
     1561 cagtgcgatg gggaacagtt tccacccaac ttccgccgcc gactgcgtgg ttttcggcat
     1621 tctgcgatct taagaggtga aacgcccaaa gccaaatacc cggctagccg attggtcgtt
     1681 cgggcgtcct tttcgtgcgc gctgcagaga gcggtgagcg cgcattatcg ctggcacttc
     1741 gggcaccacg ctgttgtgcg accgccgatc gtggcgcggc gcagcgtcgt tccgtcgcgc
     1801 ggacatttcc cgccggcctt ccaccgctca tgcagaagcc acgatttcgg cggatcggaa
     1861 aaatcccggc cgatcgtctg gagcgatgcc tgtgcgacaa accgggtcgc gcgccaaatg
     1921 gcgccgagtc tttcgacgct cagttttccc gcccgcgtcc gtggtgcgat cctggcccgc
     1981 cagagaatct catcggccat ccaattgccg actcccggaa atcctttttg cagaaggagc
     2041 acggctttaa tcggggcgcg ggcgtgccgc ctgacgaatt cccggagcca cgatttggtg
     2101 aatccgcggg agtgaatttc cggcacgctc acgtcccacc aggagggcgg ttcctttccg
     2161 tgatggagcc ggatgcgccc gaactgccgg gggtcgctga agacgagggc ccactttctt
     2221 tgctggagga caaggtggtc atgcctggcc ggatgaaatt tcgacgtctc gaggcggagc
     2281 tttccgctca tcccgaggtg aatcccgagc cagtggtcgc ccgaaaattc gaagagcatt
     2341 tgcttgccgc gtgttttgga gcggaggagg gtttgcctaa cgaggccgcg cccgagtgct
     2401 cgcacatcgg tcccgcgaaa gatccgtttt tctccatgca agtgcacggc cacgatcttt
     2461 tccccgaggc cggcgtccca gcgtttccga aaatattcga cctccgccag ttctggcatc
     2521 ggcggagatt agatcggctg cggcctccat gctacccttg gcgcgcatgg aaaaagtgat
     2581 catcgtcggg agcggctgtg cggggttgac ggcggcgatt tatgccgcgc gcgcgaactt
     2641 gagcccgctc gtgctcgaag ggcggcagcc gggcggccag ctctccacca ccacaatggt
     2701 ggaaaatttt ccgggttttc ccgaggggat tgacggcccg cagctgatca tgaacatgca
     2761 tcggcaggcc gaaaaattcg gcgcccgttt ttcctacgcc gaagcaacgg atttggagat
     2821 gacgggcgac caccttcgcc tgcaggccga cgacgaatgg ctggagacgc aggcactgat
     2881 catcgccagc ggcgcctccg cgcgctacct cggcctggag aacgaggaga aactaatcgg
     2941 ccatggccta acgagttgtg cgacctgcga cggcgcgttt tatcgcaacg tgccggtctg
     3001 cgtggtcggc ggcggcgatt ctgcggcgga agaggcgctc tttctcaccc ggtttgcctc
     3061 caaggtttat ttgatccatc ggcgcgatca gttgcgcgcc tcgaaaatca tggcggcgcg
     3121 cgtgctggcc gccagcaaga tcgaggcgat ctggaacacg gtggtgacga aatacctggc
     3181 ggacgacaaa ggagaaatga ccgcggtcga gttgcgcgac gtcgtcacca aaaaagagaa
     3241 aattctgccc gtggcctgtg tcttcgtcgc gatcggccac gacccgaaca cgaaagcttt
     3301 tgccggcaaa ctggagaccg acccggacgg ctacttgttc gttaggcacc tggccgaaag
     3361 caagcatccc ggaatcttta tcgcgggcga tgtcgccgat cgcgtctaca agcaggcggt
     3421 cacggcagcc gggtcgggct gcgcggcggc gatcgaagcg gagaagtatt tgagcgggtt
     3481 gtaggcactt tccgcgggga agcgcgagag gtgaactccg gagcagagtc gatgccgctg
     3541 agtcgagacc cgcgctccgc agagcgaaac gtcgacagga taagcgtttg aagctttgcg
     3601 atctcacgca gttttactcg ccgttgagcg gcggggtgaa gcgctatgtg cacgagaaga
     3661 tccgctacat ccagcgcgcg accgacgatg aacatgtcct gatcattccc ggaccgaaga
     3721 ccgagctgat cgaggcggag cggtcgcgca tttacgccat cggctcgccc ttgctctcgc
     3781 ggacgtcgcg ttatcgcgcg ctcctcaact tgcgcgcgat cgaggaaatt ctggaacgcg
     3841 aacagccgag cttgatcgaa tccggcgatc cctatcaggt ggcgtggaag gcgatcgcgt
     3901 ccggcgtcgg tctgcgcatc ccggtggttg gtttctatca ttcgcatttt ccggaagcct
     3961 atctccgcag cacggcgcgt tatttgggcc ggaccgcgac ggagtttgtg atggacctgg
     4021 cgcgccgcta tgtccgcaag atttacaatc agttcgaggc gacgctcgtg ccttcggcgc
     4081 gcctcgggca gctcctgacc gcctggggcg tggaaaatgt gcgcgccgtc gatctcggag
     4141 tggacgtgga gattttccac tcgcaacccg aggacggggc ggtcacgcga gactcgttag
     4201 gcatatccgc cggctgctgc ctgctgcttt acgtcggtcg gctggcccag gaaaaaaaca
     4261 cgcagacgtt gtttgccgcc ttcgagttgc tcgccgcacg cgaaccgcag cgctatcaac
     4321 ttttgattgt cggcgacgga ttgcagcgcg cggaattgcg cgccttgcag gagcgcaccg
     4381 ggacggtgac ctggcggcca tattgcaccg actccgaaga gctggcgcgg ctttatcgcg
     4441 cggcggattt gctcgtgcac ccgggtgtgc aggagacttt tggcctaacg gcgctcgaaa
     4501 gtcaggcctg cggcacgccg gtggtcggga ttcgcggcag ttacatggac cggattattt
     4561 tcggcggcca ggaacattgg gcgaaggaga attccgtccc ggcgctggcg gatgcggtcg
     4621 cggcggccgc gggcggcgag ctgcgtaaaa ccggactggt cgccgcagga agggtgcgcg
     4681 agcactacgc ctggccggtg gtttttgcac aattgtttca gatctaccgc gaagtgcggg
     4741 ccggatatca tgccgattag gacgccgggg gcggcacggc cactgcggat gcagtttgcc
     4801 ttgtgagaag cgatcggcac attaagcagc gccaagatga aaactgaccc ggcttacacg
     4861 attcgcagct accgcgcggc ggatcgcgct gcggtgcgaa ggttgtgttg cctgacggga
     4921 ttcctcggtt cgccgatcga tccggtttac gaagatcgcg aattgtttgc cgattttctc
     4981 accacctact atacggacca cgagccggag tcctcgttcg tgctcgaggt cgatggagag
     5041 ctgaagggtt atctcctcgg atcgcgcaag ccgctcctca accagcttta ttccttccag
     5101 cagaacatcg ttcttttcct ccgctcgctc ctgcgttacc cgcgttacaa cgtgcgctca
     5161 cggcgcttcc tgcgctggat gatgatgaac ggctggcgcg aagtgccggc cgccccgcgt
     5221 cgcaccccgc atttccatat caacctgctc ccggaggcgc gacaaatttc aaccacccgc
     5281 gcgctgatga gcgcctacct cagctacctc tatcgccgcg gcgagaagcg cgtttacggc
     5341 cagatcgtca ccttcgaaag ccggcggggc gagaagatgt tcgaacgtta cggcttccgg
     5401 gtgatgaacc ggtcggagat caccaagtac cgggcgttct atcccgaatc ggtttacctc
     5461 agcacggtga tcaagaatct cgaaaccgcc ggaccacttt ccgcctactc ggattcggcg
     5521 acctaacaag acctgtcggc gggcgcgggt tcgagcgcgg caattatttc tgcgaaacgg
     5581 aatgggcagc gcccatttgc aaacaagttt ttatcttctc cgcgagaatc tcgacgtgcg
     5641 tcgtgatgca ttccagatga ctgccgcgca aaggatggat cacgatattc ggcgccagat
     5701 gttgccaatc gctcgcgagc atgttttccg gcaatacgtc ttcggaaagg agcagcgcca
     5761 ccggagcatg ataaggttgc gggcgatagc cggctgacg
//
LOCUS       DATMNS010000093         5908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3288282, whole genome shotgun sequence.
ACCESSION   DATMNS010000093 DATMNS010000000
VERSION     DATMNS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5908
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3288282"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VNW28_03485"
     CDS             <1..888
                     /locus_tag="VNW28_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09017.1"
                     /translation="AIKLGKDASARASMKTGGWTEAESYRDLIKDKAVAVSLEEQNRM
                     QLTDESLEQQINETFAQHEAERQNVDLAKRLAGLHDQKEDFDGAIAWYQYAVDLTKRS
                     DPGLVRKVADLKMKQLERQIRVDEVYLTEHGESDLQFAGKMAQLETARKQRAEVLIDN
                     ARQRLDRNPTDLQLRYELGEQLTKAGEYRDALPELQRARQNPNARLKAMNLLGRCYRE
                     LGMLDLAARQLEDAARELMVMDAVKKEIVYDLGLVYEQIGDSPKYLDAMKKIYEADYG
                     YRDVATRVESSYGSIGEGS"
     gene            1048..1896
                     /gene="ftsY"
                     /locus_tag="VNW28_03490"
     CDS             1048..1896
                     /gene="ftsY"
                     /locus_tag="VNW28_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HXA09018.1"
                     /translation="MKFFKSLIQKFAGKPVDWDELEEMLIRSDLGVPMSLRIVSALQE
                     RALTQTITAQDVAEVAREEILRVLPIQPAPIRPLPAKPKAILIVGVNGTGKTTSTAKL
                     AFYFQRNRHTVLLAAADTFRAAAIEQLAIWAERLKIEMIRGQYNADPAALCYEAYQSA
                     DRRDIEFLICDTAGRLHTKSNLMAELEKIKRSLAKHDSQAPHERILVVDATTGSNALA
                     QAREFHAAIDLTGLIVTKLDGSGKGGVVIAIQNELGIPTRFVGTGEKLEDFAPFESRA
                     FIEQML"
     gene            1967..2341
                     /locus_tag="VNW28_03495"
     CDS             1967..2341
                     /locus_tag="VNW28_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09019.1"
                     /translation="MKILTLIIRSLLGLVFVVFGANGFLRFLPMPPLPHGLVGEFLHA
                     FFASGYVYAIAACQVIGGLLLLIGRFVPLGLTILGPIVVNIVLFHILLATEGLPLAII
                     VAALFLFLLWRYWAAFAAIVRP"
     gene            2430..3926
                     /locus_tag="VNW28_03500"
     CDS             2430..3926
                     /locus_tag="VNW28_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXA09020.1"
                     /translation="MIPTTKHSAAAAAGDFPPIKTGLAGFLAKFGVLKGAQRELWLTF
                     LIKFLIYTAYSVTNKTMVLWLSRDLGFSDQAAGALVGWVWAPAMTVFTLLAGSLTDAI
                     GLRRTFFLGVAICTFARSVMVVTTIPSLALICGVLPLAVGEALGTPVLLAATRVYSTT
                     QQRSIAFSIIYMVMNVAYVAAGLIFDYIRNLDLRLTLFGFQPTTHEQLFLVSLAIEVL
                     IFPAIYFLRRGEEEKARERRVAATTSGFGRRIAATVRQSATDTIQLFGRLVRQSAFAR
                     ILAFFLLIGFLKAIFLQTDYVFPKFGDRVLGLHAPVGKLANIDAILIIVLVPTIGALT
                     QRFAAYRMVVIGGAICAAGVFIMALPPVWFVPLAEGPFGQWLGHSYLGLTGSINPYYV
                     MAALYYAVFSVGEAFYSPRVYEYAAAIAPAGQEASYGALAYLPFLFGKLLVGTAGWIL
                     AAFCPAEGPRRPAMMWLIFALAATVAPVGLLVFRRYIRVPEAGRPDFA"
     gene            3979..4647
                     /gene="msrB"
                     /locus_tag="VNW28_03505"
     CDS             3979..4647
                     /gene="msrB"
                     /locus_tag="VNW28_03505"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXA09021.1"
                     /translation="MNETPDSLYQQARSRGNRGSAIWVGAAILTLIAATSLYVGYAGD
                     TPDPATQASAVVAANDVATNAKNASDKTNEIKTMSDTELRKKLTPEQYHVTRENGTEP
                     PFHNAYWDNHKPGIYVDLISGEALFASTDKFESGTGWPSFSKPIDPAHLVEKSDRSLF
                     MERNEVRSKKGNAHLGHLFDDGPAPTGMRYCINSAALRFVPIEKMKEEGYGQYLYLFD
                     KQTK"
     gene            4865..5254
                     /locus_tag="VNW28_03510"
     CDS             4865..5254
                     /locus_tag="VNW28_03510"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXA09022.1"
                     /translation="MRVPFNLGTVGHFGLAVRNPKKSAQWFERALGLKKEFDFEEGVA
                     VGNDYVTIALFKGQPSPATIDHMSFHLPDLATLRKALQHLKRIGADLEDPGDEIGPEA
                     PGSPHIGLWFHDPDGYRWELSVQGANA"
     gene            complement(5275..5646)
                     /locus_tag="VNW28_03515"
     CDS             complement(5275..5646)
                     /locus_tag="VNW28_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09023.1"
                     /translation="MVYALRGRAWKLLLNMVNIEKPQPTTMTTTFPDSALAPQPPSFG
                     FAELGTALFEPVLVVGAALFWLVALPFVALSLMLVKIWDTLVALKMGTLAQANPLILR
                     SSAPRTGPALSEGKSAKTAQI"
BASE COUNT         1161 a         1825 c         1725 g         1197 t
ORIGIN      
        1 gccatcaaac tgggcaaaga cgcttccgcg cgcgcctcga tgaaaacagg gggctggacg
       61 gaggccgaga gttaccgcga cctgatcaaa gacaaggcag tcgccgtttc gctcgaagag
      121 caaaatcgaa tgcagctcac cgacgaatcg ctcgagcaac aaatcaacga gactttcgcg
      181 cagcatgagg cggagcgaca aaatgtcgat ctggcgaagc gactggccgg cttgcatgat
      241 caaaaagagg atttcgatgg tgcgattgcc tggtatcaat acgcggttga cctaacgaag
      301 agaagcgatc ccgggctggt gcgaaaagtg gccgacctga aaatgaagca actcgagcgg
      361 cagatccggg ttgacgaggt gtatctcact gaacacggag agagcgattt gcagttcgcg
      421 ggaaaaatgg cacagctcga aaccgccagg aaacaacggg cggaagtgct catcgacaac
      481 gcgcgccaac gacttgatcg aaatccgacc gacctccaat tgcgctacga gttgggcgag
      541 cagttgacga aagccggcga gtaccgggac gcccttcccg aactgcaacg cgcccgccag
      601 aatccaaacg cgcgtctgaa ggcgatgaat ttgctcggcc gctgctatcg cgaactgggc
      661 atgctcgatc tcgcggccag gcaactcgag gacgctgccc gcgagctgat ggtgatggac
      721 gcggtcaaaa aggaaatcgt ctatgatctg gggctggttt acgaacagat cggagactcc
      781 ccaaaatacc tcgacgcgat gaagaagatt tacgaggccg attatggtta tcgtgacgtg
      841 gcaacccggg tcgagagctc ctacggcagc ataggcgaag gaagctaagt ccggcccgcc
      901 ggaagtgtct ttcccctcct cggcgtctcc gagcgagagg ccgcattttt ctttttgcga
      961 agatggaaga aggcatggag tgatgaagta ttatttgccg agtcatcgct ccgtaactcc
     1021 accacgccac cactccgccg tcaggcaatg aaattcttca agtcgctcat tcagaaattc
     1081 gccggcaagc cggtcgactg ggatgagctc gaggagatgc tcatccggag cgatcttggt
     1141 gtgccgatga gtctgcgcat cgtcagcgcg ctgcaggaac gcgcgctcac ccagacgatc
     1201 acggcgcagg atgtcgcgga ggtggcgcgg gaagaaatcc tgcgcgtcct gccgatccag
     1261 ccggcgccga ttcgtcccct gcccgccaag ccaaaagcga tcctgattgt cggcgtaaac
     1321 ggtaccggta agaccacctc gacggccaag ctggcttttt attttcagcg caaccggcac
     1381 accgtcctgc tcgcggcggc cgatactttc cgcgcggcgg ccatcgagca gctcgcgatc
     1441 tgggcggagc gtctgaagat cgagatgatc cgcggccaat acaatgccga tccggcggcg
     1501 ctctgttacg aggcctacca atctgcggac cggcgcgaca tcgagtttct tatctgcgat
     1561 accgccgggc gtctccacac caaaagcaac ctgatggcgg agctggagaa aattaaacgt
     1621 tcgctcgcga agcatgattc gcaggcgccg cacgaacgga tcctggtggt cgacgcgacc
     1681 accggcagca atgcgctcgc gcaggcgcgt gagtttcatg cggcgattga tctcaccgga
     1741 ctcattgtga ccaagctcga cggcagcggc aaaggcggcg tcgtgatcgc gatccaaaac
     1801 gaactcggca ttccgacccg tttcgtcggc accggcgaaa agctggagga tttcgcgccc
     1861 ttcgagagtc gcgctttcat cgagcaaatg ctttgagcaa tgcgctggga agccagcgtc
     1921 gccttttctc tcctcaaccc gcgtcagttc tggaataaca gcgcctatga agatcctcac
     1981 cctgattatc cggtctctgc tcggcctcgt ctttgttgtc tttggcgcga atggattcct
     2041 gcgcttcctc cccatgccgc ccctgcccca cggcttggtg ggcgagttcc tgcacgcctt
     2101 cttcgccagc gggtatgtct acgccatcgc ggcctgccag gttatcggcg ggctcctcct
     2161 gctcatcggc cgctttgtcc ctctcggact caccatcctc gggccgatcg tggttaacat
     2221 cgtcctcttc catatcctcc tcgcgacgga aggcctgccg ctcgcgatta tcgtcgcggc
     2281 cctcttcctt ttcctcctct ggcgctattg ggccgccttc gccgccatcg tacggccata
     2341 gtccctcgac cgcgattcgt taggccaacg aacgccgcgt tgtgagatgg cgcttccatt
     2401 ccgatgattt gcgctgattc ttttccccgg tgattccgac gacgaaacac agcgcggccg
     2461 cggccgcggg cgattttccg ccgatcaaga cgggcctggc cggattcctg gccaagttcg
     2521 gcgtgctcaa aggggcgcaa cgcgagctgt ggctcacgtt cctgatcaag ttcctcatct
     2581 acaccgccta ctcagtcacg aacaagacca tggtgctgtg gctctcgcgc gaccttggct
     2641 tcagcgatca ggcggcgggg gcgttggtcg gctgggtctg ggcgccggcc atgacggtgt
     2701 tcaccctgct cgctggctcg ctcaccgatg cgatcggttt gcgccggacg tttttcctcg
     2761 gggtcgccat ctgcaccttc gcgcgcagcg tcatggtcgt gaccacgatt ccatcgctcg
     2821 cgctgatttg cggcgtgctg ccgctcgccg tgggcgaggc gcttggaaca ccagtgctgc
     2881 tggcggcgac gcgggtttat tcgaccaccc aacagcgctc gatcgcgttt tcgatcatct
     2941 acatggtgat gaacgtcgct tacgtcgcgg ctggattgat ttttgattac atccggaatc
     3001 tggatctgcg cttgaccctc tttgggttcc aaccgacgac gcacgagcaa ctcttcctcg
     3061 ttagcctggc gatcgaggtc ctgattttcc ccgcgattta ctttctccgc cgcggggaag
     3121 aggaaaaagc gcgcgagcgg cgggtggctg cgacaacatc cggctttggg cgacgcatcg
     3181 ccgcgacggt gcggcagagc gcgaccgata cgatccagct cttcggacgg ctcgttaggc
     3241 agtcggcctt cgctcgcatc ctcgccttct ttctcctgat cggatttctc aaagccatct
     3301 tcctgcaaac ggattatgtc tttccgaagt ttggcgaccg cgtcctggga ttgcacgcgc
     3361 cggtcggcaa gctggcaaac atcgatgcca ttctcatcat cgtgcttgtg cccacgatcg
     3421 gggcgctgac gcaacgcttc gccgcttatc gcatggttgt gatcggtggc gccatctgcg
     3481 cagcgggagt tttcatcatg gcgttgccgc cggtttggtt cgtgcctttg gcggagggcc
     3541 cgtttgggca atggctcgga cacagctatc tcgggctcac cggaagcatc aatccctatt
     3601 acgtcatggc tgcgctgtat tatgcggtct tctccgtcgg cgaagcgttt tattcgccgc
     3661 gcgtctacga gtacgcggcc gccattgcgc ccgccggcca ggaagcctcc tacggcgcgt
     3721 tggcttatct tccgtttctc ttcggcaagc tcctcgtcgg cacggccggc tggatcctcg
     3781 cggccttctg tcccgcggaa gggccgcgcc gacccgcaat gatgtggctc attttcgccc
     3841 tcgcggccac cgtcgcgccg gttggattgc tcgtcttccg ccgttatatc cgggtgcccg
     3901 aagccggccg accggacttt gcctgacaac gaaaccgttg ccggggaata aatcggatct
     3961 aaaattatcc tacctgcgat gaacgaaaca cctgattcac tttatcagca ggcgcgcagc
     4021 cggggaaacc gcggatctgc catctgggtg ggcgccgcga tcctgacgtt gatcgctgcg
     4081 acgtcccttt atgtcggcta tgcgggcgac acgccggatc cggcaaccca ggcgtctgcc
     4141 gtggtggcgg ccaacgatgt cgcgaccaac gccaaaaatg cttccgataa aaccaacgag
     4201 atcaaaacca tgtctgatac cgaactgcgc aagaaactca ctcccgagca atatcacgtg
     4261 acccgcgaaa acggcaccga gccgccattt cacaacgctt attgggacaa ccataaaccc
     4321 ggcatttacg tcgacttgat cagcggcgaa gcgcttttcg cttcgaccga caagtttgag
     4381 agcggcaccg gctggccgag ctttagcaag ccaatcgatc ccgcgcatct cgttgaaaaa
     4441 agcgatcgga gcttgttcat ggagcgcaac gaagtccggt ccaagaaggg caacgcgcat
     4501 ctaggccatc ttttcgatga cgggccggcg ccaaccggca tgcgctattg catcaattcc
     4561 gccgcgctac gcttcgtgcc gatcgagaag atgaaagagg agggctacgg ccagtatctt
     4621 tatctcttcg acaagcagac gaagtagttg cgccatcggc cgaaccacgg cggccatcac
     4681 gcgcgaaatc gggacatcgc gcggaggggc gagcggcccg gctgactgct cggaataaca
     4741 cctcgggttg cccgacttgg ccgcttattg ctcgacagtg gaagcgtcaa ccgaaaccac
     4801 acctgttccg cgcgcctccc ccgtccgcac caaccgcggg ccgcgattct ctttgcagac
     4861 aatcatgcgc gttccattta atctcggcac cgtcggtcac tttggcctgg ccgtgcgcaa
     4921 tccgaaaaag agcgcgcagt ggttcgagcg cgcgctggga ctcaagaaag agttcgattt
     4981 cgaggagggc gtcgcggtcg gcaacgatta cgtcacgatc gcgcttttca agggccaacc
     5041 ctcgccggca accattgacc acatgtcgtt tcatctgccc gacttggcaa ccttgcggaa
     5101 agcactccag cacctcaaga gaatcggtgc cgacctcgag gatcccgggg acgaaattgg
     5161 cccggaagcg ccgggttcgc cgcacatcgg cctctggttc cacgatcccg acggctatcg
     5221 ctgggagcta tctgtccaag gggcgaacgc ttagttgccg aacccgcaag gcaactagat
     5281 ttgcgctgtc ttggccgact tcccttcgga aagggccggg ccggttcttg gcgcgctgct
     5341 ccggaggatg agcggattgg cctgtgcgag cgtgcccatt ttcagcgcca ccagcgtgtc
     5401 ccagatcttg accaacatca aagagagcgc cacgaaaggg agcgcgacca accagaacag
     5461 ggcagcgccc acgaccagca cgggctcgaa cagggcggtt ccgagttcgg cgaatccgaa
     5521 cgatggtggt tgaggggcga gtgcggaatc aggaaaggtg gttgtcatgg tggttggctg
     5581 tggtttctct atattcacca tgtttaaaag cagcttccat gcccgaccgc gtaaggcata
     5641 gaccacagat ccggtgcccc gctacctcgc gtctcccgct cgtttcggga tcccggcgcc
     5701 agcttgtcgc ccagcccaat atcaggcacc gctcattgga gcgagtggcg gcgagggatc
     5761 ccgcctccga cgctggctcg tagtctgagc gaatcgaaca actgacaagc gggagctccg
     5821 gtcccactcc tctatctccc cttactgctt gaactcgatc ttgaaggtcg cagattgcca
     5881 ggtgcgaaaa acggccctga tcggattt
//
LOCUS       DATMNS010000094         4963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3648344, whole genome shotgun sequence.
ACCESSION   DATMNS010000094 DATMNS010000000
VERSION     DATMNS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4963
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3648344"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1441
                     /locus_tag="VNW28_03520"
     CDS             218..1441
                     /locus_tag="VNW28_03520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXA09024.1"
                     /translation="MSKRTIFIAGGCLLLIGAWWACHRGDSAAQSGQATGSERHGGGA
                     GGSVPVVAGKVEQKDVPIYLEGLGTVQAFNTVTVHSRVDGALEKVLFKEGQDVKTGDL
                     LAQIDPRPFQEALDQAVGKKGQDAAQLANAQATFARNTDLLKKQVIDQQDFDTAKAQS
                     GQFGAAVQSDQAAIESAQTQLDYTQIKSPIDGRTGLRLVDVGNIVHAADAGGIVVITQ
                     LKPISVVFTVPEQDLQKLLNQGGETSGLPVAALDRGNTTPLGEGTLAVVDNEIDQTTG
                     TVKLKATFPNEDLKLWPGKFVNARLILKTQKNATVVPASVVQRGPQGTYAYVIKPDKT
                     VGMQAVKVGPTEDNQTVIESGLTPGQQVVVDGQYKLQPGSRVELTSATGQPQKPTGAP
                     NGKSPDGKSRRPAKS"
     gene            1438..4650
                     /locus_tag="VNW28_03525"
     CDS             1438..4650
                     /locus_tag="VNW28_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HXA09025.1"
                     /translation="MSISEPFIRRPVATSLLMVGVILLGLLGYKLLPISALPTVDFPT
                     IEVTTIYPGASADVMVSSITTPLERQFGQISGLASMNSTSSFGTSTITLQFVLDRPID
                     VAAQDVQAAINVAGGYLPKDLPNPPKYNKVNPADTPILTLAITSDSLPLAQVNDSADT
                     LLAQKLSEVTGVGLVTIEGNQKPAVRVQINPAALSSLGLSLEDIRTILGQANVNSPKG
                     SFNGPQQSFTIGSNDQIFSADAYKPIIVAYKNGAPIRLGDVANIIDSVENDQLGAWVG
                     NKQGEQPAVLLDIQRQPGANIIQTVNSVKALLPKLTGTLPPSVHVSILADRTETIRAS
                     VSDVQFTLLLTIALVVMVMFLFLRTLWATVITSVALPLSLIGTFGIMYLAGFSLDNLS
                     LMALTISTGFVVDDAIVMIENIVRFIEAGDAPLEAALKGARQIGFTVVSLSVSLIAVF
                     IPLLFMTGIVGRLFREFAITMSVAVVVSAFVSLTLTPMMCAKLLKPKTDEDRAHENRF
                     LRATEGYFERFRNLYERGLRWVMKHQRFTLITFFITFVVTIFLYLVIPKGLLPQQDTG
                     VIIGVTDAAEDISFSAMTARVHAISDLVRQDPEVANVSASVGAGTVNATINTARLFIV
                     LKPHNERSASAQEIIDRLREKTRNVEGISLYLQAAQDLQIDSRVSRTQYQYILQDADA
                     AELAEWTPKLVHQLGQLPELADVASDQQVNGLQLNVDVDREKASRLNVLTQAIDDTLY
                     DAFGQRQVSIIFTQLNQFRVILEVEPNFRSSPNVLDKIYVKSSTGQPVPLSAFATMKV
                     SNTPLSIPHQGQFPAATISFNLRSGESLSNAIPAIEKAEQQIGLPDTIATTFSGAAAE
                     FRSSLTTEPLLILAAVIVIYIVLGVLYESYIHPITILSTLPSAGVGALLALLICRTDF
                     SLVALIGIILLIGIVKKNAIMMIDFALEAERNDGMTPEESIYQACLLRFRPIMMTTAA
                     ALLGALPLALEHNTGSELRRPLGIAIVGGLLLSQFLTLYTTPVIYLYMERARSWFEER
                     RHRGRLEQTQLPLRTDEKREPEIASVGANGSRNGGARTLR"
     gene            4647..>4963
                     /locus_tag="VNW28_03530"
     CDS             4647..>4963
                     /locus_tag="VNW28_03530"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXA09026.1"
                     /translation="MSDQRSAEDFAGRVIIVTGAWRGLGRAAAERFHERGGAVAVNVR
                     DRARAEDLAASIGDRTLAVPGDLAATGAAEEIVRMTLERFGRVDVVVNNAALALSTRF
                     LDLT"
BASE COUNT         1009 a         1590 c         1443 g          921 t
ORIGIN      
        1 ccgacaaaat cacgcagcca atccgggcag ccgatattgt ttgaattcct cgacgatcaa
       61 tctggcgcca atggcattga ctggagaaca aatcccgggc tttgccccca aggcaagcca
      121 tccatgcgat tcccatttaa ccagaagatt ttcgcaacct gaaagcaatc ccggctgtca
      181 tagaggaagc taccttcgac gcacgacgcg ccatataatg tctaaacgaa ccattttcat
      241 cgccggagga tgcctgctgc tgatcggcgc ctggtgggcc tgccatcgcg gcgatagcgc
      301 ggcgcagtcg ggccaggcga ccggcagcga acgccatggc ggaggggccg gcggctcggt
      361 cccggtggtc gccggcaagg tcgagcagaa ggatgtgccg atttatctcg aggggctcgg
      421 gacggtgcag gcgtttaaca ctgtgaccgt gcactcgcgc gtggatgggg cactggagaa
      481 ggtgcttttc aaggaagggc aggacgtcaa aaccggtgac ctgctggcgc agatcgatcc
      541 gcgcccgttt caggaagcgc ttgatcaagc agtgggcaag aaagggcagg acgccgccca
      601 gcttgccaac gcgcaggcga cgttcgcccg caacaccgac ctgctgaaaa aacaggtgat
      661 cgatcaacag gacttcgaca ccgcgaaagc tcaatccggg caattcggcg cggccgtgca
      721 gtccgatcaa gccgcgatcg agagcgcgca gacccagctt gattacaccc agatcaaatc
      781 gccgatcgac gggcggacgg gacttcggct ggtcgatgtc ggcaatatcg tccacgccgc
      841 ggatgcgggc gggatcgtcg tcatcacgca actgaaaccg atctcggtcg ttttcaccgt
      901 gccggagcag gatttgcaga aattattgaa ccagggcggc gagacgagtg gtcttccggt
      961 cgccgctctc gatcgaggca acaccacgcc gctcggcgaa ggaacgctcg ccgtggtcga
     1021 taacgagatc gatcaaacga ccggcacggt gaagctaaag gccactttcc ctaacgagga
     1081 cctgaaattg tggccgggaa aatttgttaa tgcgcgactc attctgaaga cgcagaaaaa
     1141 cgcgaccgtc gtgcctgcga gtgtggtgca gcgcgggccg caaggcacct acgcctacgt
     1201 gatcaagccg gacaaaacgg tcgggatgca ggcggtgaaa gtcgggccaa cagaagataa
     1261 ccagacggtg atcgagagcg gcctgacgcc cggtcaacag gtcgtcgtcg atggccagta
     1321 taagttgcag cccggctcgc gcgtggagct gacctccgcc actggacagc cgcaaaagcc
     1381 aactggcgcg ccgaatggca aatccccgga cggcaagtcg cgccggcctg cgaaatcgtg
     1441 agcatctccg agcccttcat tcgccggccg gtcgcgacct cgctgctcat ggtcggcgtg
     1501 atcttgttag gcttgctcgg ctacaaattg ctcccgatct ctgccctgcc cacggtcgat
     1561 ttcccgacga tcgaggtgac gacgatctac cccggagcaa gcgccgacgt gatggtctcg
     1621 tcgatcacaa cgccgctcga acggcagttc gggcagatca gcgggctcgc ctcgatgaat
     1681 tccacgagct cgtttggaac ttccacgatc acgctgcaat tcgtgctcga tcgcccgatc
     1741 gatgtcgccg cgcaggacgt gcaggcggcg atcaatgtcg ccggcggtta tctcccgaaa
     1801 gacctgccca atccgccgaa atacaacaag gtcaatcccg ccgacacacc gatccttact
     1861 cttgcgatca cctcggacag tttgccgctc gcgcaggtca acgactccgc cgacaccctc
     1921 ctcgcacaga aactgagcga agtcaccggc gtcggcctcg tcacgatcga gggcaaccaa
     1981 aaaccggccg tgcgcgtgca gataaatccg gccgcccttt cctcgttagg tctgagcctc
     2041 gaagacatcc gcaccatcct cggtcaggcg aatgtgaatt cgccgaaagg cagttttaac
     2101 gggccgcagc aatcgttcac gatcgggtcc aacgaccaga tattttctgc cgacgcttac
     2161 aagccgatca tcgtcgccta caaaaacggc gcgccgattc ggctcggcga cgtcgccaat
     2221 atcatcgaca gcgtggagaa cgatcagctc ggcgcgtggg tcggcaacaa gcagggcgag
     2281 caacctgccg tgctcctcga catccagcgg caaccgggcg caaacatcat ccaaaccgtc
     2341 aacagcgtga aggcgctcct cccgaaactg accggcacac tcccgccttc ggtgcacgtt
     2401 tcgattctgg ccgaccgcac ggaaacgatc cgcgcctcgg tcagcgacgt gcagtttacc
     2461 ctgctcctca cgatcgcgct cgtcgtcatg gtgatgttcc tttttctgcg cacgctctgg
     2521 gcgaccgtga tcacgagcgt ggcgctgccg ctgtcgctta tcggcacctt cggcatcatg
     2581 tatctggccg ggttcagcct cgacaacctg tccttgatgg ccctcacgat ctcgactggc
     2641 ttcgtcgtgg atgacgcgat cgtgatgatc gaaaacatcg tgcgctttat cgaggcgggc
     2701 gacgcgccgt tggaagccgc gctcaaaggc gcacgccaga ttggattcac cgtcgtctcg
     2761 ctcagcgtct cgctcatcgc ggtcttcatt ccgctcctct tcatgaccgg catcgtcggt
     2821 cgcttgttcc gtgaatttgc catcaccatg tcggtcgcgg tcgtcgtttc ggcattcgtt
     2881 agtcttacgc tcaccccgat gatgtgcgcg aaattactca agccgaagac ggacgaagat
     2941 cgcgcgcacg agaaccgttt cctgcgcgcg acggaaggct atttcgagcg tttccgcaat
     3001 ctctacgaac ggggcttgcg ctgggtcatg aagcaccagc gcttcaccct gatcaccttt
     3061 ttcatcacct tcgtggtcac gatttttctc tacctcgtga tcccgaaagg actgcttccg
     3121 cagcaggaca ccggagtgat catcggcgtg accgatgccg ccgaagatat ctcgttctcc
     3181 gccatgacgg cgcgcgtcca tgccatttcc gatctcgtca ggcaggatcc cgaggtggcg
     3241 aatgtctcgg cttccgtcgg cgccggcacg gtcaacgcca cgatcaacac cgcgcggctc
     3301 ttcatcgtgc tcaagccgca taacgaacga agcgcgagcg cgcaggaaat catcgaccgg
     3361 ctacgcgaga aaacgcgcaa cgtcgaagga atttcgctct acctgcaggc cgcgcaggat
     3421 ttgcagatcg attcgcgcgt cagccgcaca caataccaat atattttgca ggatgccgac
     3481 gccgcggaac tggcggagtg gacgccgaaa ctggtccacc aactcgggca acttccggag
     3541 ttggccgacg tcgcgagcga tcagcaggtc aacggattgc agctcaacgt cgacgtcgat
     3601 cgcgagaagg cctcacgcct caacgtcctc acccaggcga tcgacgacac gctttacgat
     3661 gccttcggcc agcgccaggt ctcgatcatt ttcacccagc tcaatcagtt ccgcgtcatc
     3721 ctcgaagtcg aaccaaactt ccgctcctcg ccgaacgtgc tcgacaagat ttacgtgaag
     3781 tcgagcaccg gccaaccggt gccgctgagc gcgtttgcga ccatgaaggt gagcaacacg
     3841 ccgctctcca ttccgcatca ggggcagttc cccgccgcga ccatctcctt caatctccgc
     3901 agtggcgaat cgctcagcaa cgccatcccg gcgatcgaaa aggccgagca gcagatcggc
     3961 ctgccggaca cgatcgcgac gaccttcagc ggcgcggcgg ccgaatttcg ttccagccta
     4021 acgaccgagc cgctgctcat tctcgcggcc gtcatcgtca tctacatcgt cctcggcgtt
     4081 ctctacgaga gctacattca tccgatcacg attctctcga cgctgccgag cgcaggcgtc
     4141 ggagcgctcc tcgcccttct catctgccgg accgacttct cgttagtcgc gctcatcggc
     4201 atcattctcc tgatcggcat cgtgaagaag aacgcgatca tgatgattga tttcgccctg
     4261 gaagccgaac gcaatgacgg catgacgccc gaggaatcaa tctatcaggc gtgtctcctg
     4321 cgcttccgcc cgatcatgat gacgaccgcg gccgcgttgc tcggcgcatt accgctcgcg
     4381 ctcgaacaca acactggcag cgagttacgc cggccgttag gcatcgcgat cgtcggcggc
     4441 ctgctccttt cgcaatttct caccctttac acgactccgg tgatttatct ctacatggaa
     4501 cgggcgcgga gctggttcga ggagcgccgt catcgcggcc ggctcgagca gactcagctg
     4561 ccgctgcgga cggatgaaaa gcgcgagccg gagatcgcga gtgttggagc gaatgggagc
     4621 cgcaacgggg gagcgcggac attgagatga gcgatcagcg ttcagcggag gacttcgccg
     4681 ggcgcgtaat catcgtcacc ggcgcctggc gcggactcgg gcgtgcggcg gcggagcgct
     4741 ttcacgaacg aggcggcgcg gtggcagtca atgtgcgcga ccgcgcgcgg gccgaggatc
     4801 tcgccgcgtc gatcggcgac cgcacgctag ccgtcccagg tgacctcgcc gcgaccggtg
     4861 cggcggagga gatcgtgcgg atgacacttg aacgctttgg ccgcgtcgac gtcgtcgtga
     4921 acaacgccgc gctcgcgctc tccacgcggt ttctcgatct gac
//
LOCUS       DATMNS010000095         7819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_489570, whole genome shotgun sequence.
ACCESSION   DATMNS010000095 DATMNS010000000
VERSION     DATMNS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7819
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489570"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VNW28_03535"
     CDS             <1..855
                     /locus_tag="VNW28_03535"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXA09027.1"
                     /translation="GCQPPRVQAKPWQEFAGEKALAQVQALVDFGPRPPASAALERER
                     VYLRKNLEASGWKVETQPFTDQTPRGPIAFVNLIATWPGKQAAATPRFLVCSHYDTKT
                     FDTITFVGANDGGSSTGALLEMARVLSLHPELAAKIELVFFDGEEAYEQFSATDGLYG
                     SRYFARQITASKTEKMYRGGILWDMMGDRDLTITLPPDSPVELARGIFAAADALQVRN
                     QFTYFSQDILDDHSPLNAIGIPTIDLIDFDYPPWHTAGDTMDKLSAQSLQTVGAVTLY
                     YLSELAFK"
     gene            1077..2216
                     /locus_tag="VNW28_03540"
     CDS             1077..2216
                     /locus_tag="VNW28_03540"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXA09028.1"
                     /translation="MIAARPNQFELAFATSHTWREQKMARQKSKNVGIIGLGIIGSRV
                     AGALRKRGLQVFVWNRTPRPEQNFVGSPGEVAELCDVVQIFVSDDEALLGIVRQLGPA
                     LTAQHVVTAHCTVSPESMRAAAELVERRGARFLEAPFTGSKVAAENGQLVYYIGGDEA
                     TLKQARPILEASSKEIVLIGEKIGDATIMKVATNIVTAATAQVAAEALALIRKAGISP
                     ELFGRAMKSNASNSATLEMKVPMMLAGDFEPHFSVKHMLKDVRIAAHLARDYSLDLPV
                     TEVASDVLLAELKQGRGDADYSSVAQKYFPAPAKIVRPEPEKKAEPETKEPPEASPDG
                     PPTGRKRPAWARPAEPKPEPPAEPEKAPEAPNLSGGIRGWFSRRS"
     gene            2237..3112
                     /locus_tag="VNW28_03545"
     CDS             2237..3112
                     /locus_tag="VNW28_03545"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HXA09029.1"
                     /translation="MPLFDLSDRLKLRLTGDDRVRFLNGQVTNDVRKANANLSMAACI
                     LSAKGKMDALIFIGAGPDELIVDADAELKARLAARLERYLIADEVTLEDVTERFALFH
                     VTGESAPTLPNDARWRRGKRFGVAGWDLFVPAAERESVRQLLAAQDPICDPECAERLR
                     IEQGIPRWGRELTGQIIPVEAGLEGEAIDYAKGCYIGQEVISRMKISGQTNKRLCGLV
                     SLDGSPLAPGMRLYPAAKEREMGWITSATRSESLGKEIALGFVKRSANEAGTMLSART
                     THGATMVEVVALPFR"
     gene            3278..4411
                     /gene="ugpC"
                     /locus_tag="VNW28_03550"
     CDS             3278..4411
                     /gene="ugpC"
                     /locus_tag="VNW28_03550"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HXA09030.1"
                     /translation="MAKVSVKNVSKIYPGEKGGNVTAVKDVSLEIADREFVVLVGPSG
                     CGKSTTLRMIAGLEEISQGDVYIGDKRVNDVAPKDRDIAMVFQNYALYPHMNVYDNLA
                     FGLKLRKYPKTEIKRRVVDAASILGIEALLERRPKALSGGQCQRVAVGRAIVRQPKVF
                     LFDEPLSNLDARMRVQMRTEIARLHQRLQATMIYVTHDQVEAMTMGDRIVVMNDGVVQ
                     QHDTPRVLYNAPVNLFVAGFVGSPPMNFITGTLKAEGDKIRFREIEGGTIEVAFPAAD
                     RPGAREFIGQNVILGIRPENIEVATLSRKEGKAAATGFPAIVDIVEPMGAETNLYLQT
                     GAHTLVCRSRGAFEHHEAGHRLQFEMALAKAHFFDPVSTNRLS"
     gene            4454..5164
                     /locus_tag="VNW28_03555"
     CDS             4454..5164
                     /locus_tag="VNW28_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09031.1"
                     /translation="MRSTARLTYKAKPALETLEPETTVNQRLLAEIGRTQRLEILNTL
                     KRTRGLSVNQLVTKMKMSYMGIKQHCLTLERDGYLDTWRRPQKMGRPEMVYRLTRRSH
                     GLFQADSNVFTLDLLQSIEEIHGANAPEKLLYNLFERKTRALKEKINGNTVEERAKSL
                     AAIRDEEGHMSQLVNEPTEGGTQILECHSPIMNLIERYEIIGRLEQDMFEKLLQTPVR
                     REETRTSGLYEAAFYFAV"
     gene            5324..7288
                     /locus_tag="VNW28_03560"
     CDS             5324..7288
                     /locus_tag="VNW28_03560"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-1,4-glucan--maltose-1-phosphate
                     maltosyltransferase"
                     /protein_id="HXA09032.1"
                     /translation="MPSQFPAAVIENLEPLIDGGRYPIKRVVGEDVVVEADIFKDGHD
                     IVAAALKWRAAGEARWHETAMKHVDNDRWRGAISLYENATYEYTVEAWTDVFAGWQHE
                     FTAKFEAGLANLTSEKLEGAALLAAAAKRARGADAKRLREFAEQMRAGSDALVHRLAQ
                     AGELEVLMATYAARSGATQYSPPPRVIVDRVAARTAAWYEFFPRSAEGRADRGSTFRD
                     CLARVEDAQAMGFDVIYFPPIHPIGLTNRKGRNNSVTCRPGEPGVPYAIGNRNLGCPN
                     GGGHRDIEPSLGTFADFAWLEKQIRRRGMEIALDFALNCSPDHPYVAEHPEWFYKRPD
                     GTIKYAENPPKKYEDIYPLNFRCANWQELWEEMKSIILFWAEHGVRIFRVDNPHTKPV
                     VFWEFLISGVREKFPDTIFLSEAFTRPKMMKALAKAGFTQSYTYFTWRTGKQELTEYF
                     TELTQTRMREYFRGNLFTNTPDILPLHLQTGGRPMFQIRAVLAATLSSVYGIYSGFEL
                     CENAALPGREEYADSEKYQFKERDWDAPGNIKELITSLNRIRRENRALHFYDNLRFHP
                     AENDAILFYSKMTRARDSIILIVVNLDPSFTQSGFVHVPIEDFGEIGSESYEVHDLLT
                     DARYVWQGKRNYVALHPGVQPAHIFRVRRL"
     gene            7420..>7819
                     /locus_tag="VNW28_03565"
     CDS             7420..>7819
                     /locus_tag="VNW28_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09033.1"
                     /translation="MKSFLSLLVVFASAGPALAQAPASEPPILPRTTNILEAMIAAGP
                     VMLLLFLLSVFSVMLIIVYFFTIRRGAVVTSGYMATADALLRKRDYLGLLAVSNRHGE
                     AIARVVQKTLDFMTKNPAADFAQAREIAETE"
BASE COUNT         1591 a         2428 c         2380 g         1420 t
ORIGIN      
        1 ggttgccagc cccctcgcgt ccaggccaaa ccgtggcagg aatttgccgg cgaaaaagcg
       61 ctcgcccagg tccaggcact ggtcgatttc ggaccgcggc cgccggcctc tgccgcgctc
      121 gagagagaac gcgtttacct gcgaaaaaac cttgaagcat ccggctggaa ggtagagacc
      181 cagcctttca ccgaccaaac gccgcgcggc ccgattgctt ttgtgaacct gattgccacc
      241 tggcccggca aacaggcggc cgcgacgccg cgcttcctcg tctgttcgca ctacgacacc
      301 aagaccttcg acacgatcac cttcgtgggc gcgaatgacg gcggctcgag caccggcgcg
      361 ctcctcgaga tggcgcgcgt cctcagcctg catccggagc tggccgcgaa aatcgagctc
      421 gtttttttcg acggcgagga agcctacgag caattcagcg cgaccgatgg tctctacggc
      481 agccgctact ttgcccgcca gatcaccgca tcgaaaaccg aaaaaatgta tcgcggcggc
      541 atcctctggg acatgatggg cgaccgcgac ctcaccatca cgctgccccc ggattcgccg
      601 gtcgaattgg cgcgtggcat cttcgccgcg gccgacgccc tgcaggtgcg caaccaattc
      661 acctacttca gccaggatat tctcgacgat cactcgccgc tcaacgcgat cggcatcccg
      721 acgatcgacc tgatcgattt cgattatcct ccctggcaca cggccggcga cacgatggac
      781 aagttgagcg cgcagagcct gcaaacagtc ggcgcggtca cgctctatta tctgtccgag
      841 ctggcgttca aataaagcgg gcgctgctcg ctgcgacttt cccgctcacg ctcggcgtcc
      901 cgaccgggga attccttacc tgcgacgcac tattagtccg cagcttcgag gaggcccggc
      961 ccgaaatcag cgcgcgtctt gagaaccaga aacgtgcgag caccgttgcc gacctgctcg
     1021 cggcacggcc atgatccgcc gcgcggggtc atcgacaaaa tgcagggacg caattcttga
     1081 ttgcagcgcg gcctaatcaa tttgagcttg ccttcgcaac ttcccacacc tggagagaac
     1141 agaagatggc ccgccaaaaa tccaaaaacg tcggaatcat cggactcgga atcatcgggt
     1201 cgcgagtggc cggcgctctg cgcaagcgcg ggcttcaggt ctttgtttgg aatcgaactc
     1261 cgcgcccgga acaaaacttt gtcggttcgc cgggcgaggt cgcggagctc tgcgatgtag
     1321 tgcagatttt tgtttccgac gacgaggcgc tcctcgggat cgtgcggcaa ctcggccccg
     1381 ctctgacggc ccagcacgtc gttaccgctc actgcacggt ctcgccggag agcatgcgcg
     1441 cggccgccga gctggtcgaa aggcggggtg cgcgctttct cgaggcccct ttcaccggga
     1501 gcaaagtcgc ggcggaaaac ggccagctcg tctactatat cggcggcgac gaggcgaccc
     1561 tgaaacaggc gcgcccaatc ctggaggcga gtagcaagga gatcgtcctc atcggggaaa
     1621 aaatcgggga cgcgaccatc atgaaagtcg caaccaatat cgtgacggcc gcgaccgcgc
     1681 aggtggcggc ggaagcgctc gcgctcatcc gcaaagccgg aatttctccg gaacttttcg
     1741 ggcgcgcgat gaagagcaat gcgagcaact cggccaccct ggagatgaaa gtgccgatga
     1801 tgctggccgg cgattttgag ccgcactttt cagtcaagca catgctcaag gacgtccgca
     1861 tcgcggccca cctggcacgt gattattccc tcgatctgcc ggtgacggaa gtggcgagcg
     1921 acgtgctgct cgccgagctg aaacagggcc ggggcgacgc ggattattcc tcggtggcgc
     1981 aaaaatattt tccggctccg gccaagatcg ttaggccgga gccggagaag aaagcggaac
     2041 cggagacgaa ggagccgccc gaagcgagcc cagatgggcc accgaccggg cgcaagcgac
     2101 ctgcctgggc gcggcccgcc gaaccgaaac cggagccgcc ggctgaaccg gagaaagccc
     2161 cggaagctcc caatttatcc ggtggaattc gcggctggtt tagccgccgt tcctaacgaa
     2221 cgcgccggcc aatctgatgc ccctgtttga tctctccgat cgccttaaac tgcgtctgac
     2281 cggggacgac cgggtgcggt tcctgaacgg gcaggtgacg aatgacgtcc gcaaagccaa
     2341 cgccaacctg tcgatggcgg cctgcatcct gagcgcgaaa gggaaaatgg atgcgctcat
     2401 ttttatcggc gcggggccgg atgaattaat cgtcgatgcc gacgcggagc taaaggcaag
     2461 actcgcggcc cggctcgagc gttatctcat cgccgatgag gttacgctcg aggacgtgac
     2521 ggagcggttc gcactttttc acgtcaccgg ggaatccgcg ccgaccttgc ctaacgacgc
     2581 tcgctggcgc cgggggaaac gattcggcgt cgccggctgg gatctcttcg tgccggcggc
     2641 ggaacgcgag agcgtgcgcc aattgctggc cgcgcaagat ccgatttgcg atccagaatg
     2701 cgccgagcgg ttgcgcatcg aacagggtat tccgcgttgg ggccgggagc tgaccggcca
     2761 gattatcccg gtggaggcag gtctcgaagg cgaggcgatt gattacgcga agggttgcta
     2821 catcggccag gaagtcattt cacggatgaa gatttcgggc caaacgaaca agcgcctctg
     2881 cggtttggtt tcgctcgacg gttccccact cgcgccgggg atgcggctct accctgcggc
     2941 aaaggagagg gaaatgggct ggatcacgag cgcgacgcgc agtgaatcgt taggcaaaga
     3001 gattgcgctc ggttttgtca agcgcagcgc gaacgaggcg ggcaccatgt tgtcggcgcg
     3061 caccacgcac ggggcaacga tggtcgaggt cgtcgcattg ccttttcgtt agggccgaca
     3121 tgcttgctct gggggggctc gggcgcctcg cccgttggag gaggcgccga acgcagcggg
     3181 cggggcgtcc gcgctccccg gaggaacagc tacacgcgct cgcggagaag tggattgacc
     3241 cctctcggcc ccctccatac tttggccctt ccgctgcatg gccaaggtct ccgtcaaaaa
     3301 cgtttccaaa atctatcccg gcgaaaaggg cggaaacgtg accgcggtca aagacgtcag
     3361 cctcgagatc gcggatcgcg agttcgtcgt gctcgtcggg ccgtcgggtt gcggaaaatc
     3421 caccaccctg cggatgatcg cgggcctcga agagatctcg cagggtgatg tctacatcgg
     3481 cgacaagcgg gttaacgacg tcgcgccgaa ggaccgcgat atcgcaatgg ttttccaaaa
     3541 ttacgcgctc tatccgcata tgaacgtcta cgacaatctc gcgttcggcc tcaagttacg
     3601 gaaatatccg aagacagaaa tcaagcgccg ggtggtcgat gcggcgagca ttttagggat
     3661 tgaggcgttg ctcgagcgga gaccaaaagc gctctccggc ggacagtgcc aaagggtggc
     3721 ggtgggccgc gccattgtgc ggcaaccaaa ggtttttctc ttcgacgaac cgctctccaa
     3781 cctcgacgcc aggatgcggg tgcaaatgcg caccgagatc gccaggctcc atcagcgact
     3841 tcaggcgacg atgatttacg tcacccacga ccaggtcgag gcgatgacga tgggggaccg
     3901 aatcgtggtc atgaatgatg gcgtggtgca gcagcacgac acgccgcgcg tgctctacaa
     3961 cgccccggta aaccttttcg tggccggctt tgtcggcagt cccccgatga atttcatcac
     4021 gggcacgctc aaggcggagg gcgacaagat tcgatttcgc gagatcgaag gcggaacgat
     4081 tgaggtggcc tttccggccg cggatcggcc cggggcgcgc gagtttatcg ggcaaaacgt
     4141 gattctcggg atccggccgg aaaatattga agtggcaacc ttgagccgca aggaaggcaa
     4201 ggcggcggcc accggatttc ccgccatcgt cgacatcgtc gaaccgatgg gagcggaaac
     4261 caatctctat ctgcagaccg gcgcccatac gctggtctgc cgcagccggg gtgcgttcga
     4321 gcatcacgaa gccggtcatc ggcttcagtt cgaaatggct ttggccaagg ctcatttttt
     4381 cgatcccgtc tcgaccaacc gcctgagcta gcgatctctt cgccgctgag gcttttccgg
     4441 cgcggagatt tgcttgcgct cgaccgcgcg cctcacttat aaagcaaagc ctgccctgga
     4501 aacgctcgaa ccggagacca ctgtgaacca acgattactc gccgaaattg gccgcactca
     4561 gcgcctggag attctcaaca cgctcaagcg aacccgcggc ctgtccgtta accagctcgt
     4621 caccaaaatg aagatgagct acatgggaat caaacagcat tgcctgaccc tcgagcgcga
     4681 cggttatctc gatacctggc gacgcccgca aaaaatgggc cggcccgaaa tggtctaccg
     4741 tctgacgcgc cgcagccatg ggctcttcca ggccgatagc aacgtgttca cgctcgatct
     4801 gttgcaatcg atcgaggaaa ttcacggcgc gaacgctccc gagaagctgc tctacaatct
     4861 cttcgaacgg aagacgcgcg cgctgaagga gaagatcaac ggaaacaccg tcgaagagcg
     4921 cgcgaaatcg ctcgcggcca ttcgcgacga ggagggacac atgtcgcaat tggtcaatga
     4981 accgaccgaa ggcggcaccc agattctgga atgtcactcg ccgatcatga atctgatcga
     5041 gcgttacgaa atcatcggcc ggctcgaaca ggacatgttc gagaaactcc tccagactcc
     5101 ggtgcggcgc gaagagaccc ggacttccgg actctacgag gcggcgtttt atttcgcggt
     5161 ctgattgtcc tagagacggg cgtgtcgccc gccggaaacc cttgcccgcg ggccgcgcgc
     5221 gcgttcttgc gggccgacac gaccgccgct ccatgggcga gcccgcattg ccggtgggca
     5281 atttaccctt tcgctacccg tggatttcag ttgattggtg gcgatgccca gccaattccc
     5341 cgccgccgtc atcgagaacc tggaacctct gattgacggc ggtcgctacc cgatcaagcg
     5401 cgtcgtgggc gaggacgtgg tggtggaagc ggatattttc aaagacggcc atgacatcgt
     5461 ggcggcggcg ttgaagtggc gggccgccgg cgaggcgcgc tggcacgaga ccgcgatgaa
     5521 gcacgtcgat aacgatcgct ggcggggggc catttccctc tacgaaaatg cgacctacga
     5581 gtacacggtc gaagcctgga ccgatgtctt cgccggctgg caacatgagt tcacggcgaa
     5641 attcgaagcc ggccttgcga atctgacgag cgaaaaactg gaaggcgcgg ccctcctcgc
     5701 cgccgccgcc aagcgcgccc gcggcgccga cgccaaacgc ctgcgcgaat tcgccgagca
     5761 aatgcgcgcc ggcagcgacg cgctcgtcca tcgcctggcg caggctggcg agctcgaggt
     5821 cttgatggcg acctacgccg cgcggtccgg cgcgactcag tacagcccgc cgccgcgggt
     5881 gatcgtcgat cgggtcgccg cgcggaccgc cgcctggtac gagttttttc cccgttccgc
     5941 ggagggacga gcggaccgcg gttcgacttt tcgcgactgc cttgcgcggg tcgaggatgc
     6001 gcaggcgatg ggcttcgacg ttatctactt tccgcccatc catccgattg gcctaacgaa
     6061 tcgcaaaggc cgcaataact ccgttacctg ccggccgggt gagccgggag tgccctacgc
     6121 gatcgggaat cgcaacctcg gttgcccgaa cggcggcggg caccgggaca tcgagccgtc
     6181 gttaggcaca ttcgccgatt tcgcctggct ggaaaaacag atccgtcgcc gcgggatgga
     6241 gatcgcgctc gatttcgcgc tcaattgctc gcccgaccat ccctatgtcg cggagcatcc
     6301 ggagtggttc tacaaacgtc ccgacggcac gatcaagtac gctgagaatc cgcccaagaa
     6361 atacgaggac atttacccgc tcaactttcg ctgcgcgaac tggcaggagc tttgggagga
     6421 gatgaaaagc attatcctct tctgggcgga acacggggtg cgaattttcc gggtcgataa
     6481 cccgcacacc aagccggtcg tgttttggga attccttatc tccggggtgc gcgaaaaatt
     6541 cccggacacg atctttctct cggaagcgtt cacccggcca aaaatgatga aagcgctggc
     6601 caaagcgggc ttcacccaga gctacaccta tttcacctgg cgcaccggca agcaggagct
     6661 gaccgaatat ttcaccgagc tgacacagac caggatgcgt gaatattttc gcggcaatct
     6721 cttcaccaac acgccggata tcctgccgct tcacttgcag accggtggcc ggccaatgtt
     6781 tcagatccgc gcggtcctcg cggcgacgct gtcgagcgtt tacggcatct acagcggctt
     6841 cgagctctgc gaaaacgccg cccttcccgg acgcgaggag tacgccgatt ccgaaaaata
     6901 tcagttcaag gaacgcgatt gggacgcgcc cggaaacatt aaagagctga tcaccagcct
     6961 gaaccggatc cggcgcgaaa atcgggccct ccatttctac gacaacctcc gattccatcc
     7021 cgccgagaac gacgcgatcc tattttacag caagatgacg cgggcgcgcg acagcatcat
     7081 tctcatcgtc gtgaatctcg acccgagctt tacgcagagc ggctttgtgc acgtgccgat
     7141 cgaggatttc ggcgagatcg ggagcgagag ttacgaggtg cacgatctcc taaccgatgc
     7201 ccgttacgtc tggcagggga agcgtaacta tgtcgcgctg catccgggcg ttcagcccgc
     7261 gcacattttt cgggtgcggc gtttatagac gccacctctg tcgggcgaga ctacgggcac
     7321 gagcaggaga agaagatgaa cgatcggacc cagaccactg tctcagcgcc ggtgagttgc
     7381 atcgtcagcc ctcgcgccta tgctaagccc gaattgagca tgaagagttt cctttcactg
     7441 ctcgtcgtgt tcgcgagcgc cggtcccgcc ctcgcgcaag ctccggcgtc ggagccaccc
     7501 atcctcccgc ggacgaccaa catcctcgaa gccatgatcg ccgccggccc ggttatgctg
     7561 ctgctttttc tcctttccgt cttctcggtc atgctgatca tcgtttattt tttcaccatc
     7621 cggcgcggcg cggtggtcac cagcggctac atggcgaccg ccgatgcgct cctccgcaag
     7681 cgcgactatc tcggcttgct cgcagtttcg aatcggcacg gcgaagcgat cgcgcgcgtc
     7741 gtccagaaga cgctcgattt catgacgaaa aatcccgcgg cggattttgc ccaggcccgc
     7801 gagatcgcgg aaaccgaag
//
LOCUS       DATMNS010000096         4474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2278611, whole genome shotgun sequence.
ACCESSION   DATMNS010000096 DATMNS010000000
VERSION     DATMNS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2278611"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..1926
                     /locus_tag="VNW28_03570"
     CDS             37..1926
                     /locus_tag="VNW28_03570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HXA09034.1"
                     /translation="MTPRPRFTWTQPNHRVLLLFAVICGLSTFSAFLLQRLGAPALGA
                     IPLFIVAYLSGGWFASIQLSGELRRGKWDINLLMLVVALGAASVGAWAEGGTLLFLFA
                     LSNALERFANHRTEQTISSLLKSAPETAWRRENGRWIEVAADSLGIGDEVLVKPGELF
                     PVDGEVIEGATSVDESALTGEALPVSKRAGDPVSGGTMNLEGQAVLRVQRLPSESAVQ
                     RIIDLIESAREQKAPAQRFTDTFTRYYTVVVLTGSVVFLGWLLLWVREPFAQAVYRMM
                     TLLVVSSPCALVLSIPSAILVAIAAGARQGILFRGGVAVETLAGVNHFAFDKTGTLTT
                     GELEVARIETTGEISREEALAVAAAVAQNSTHPLSRAVVAEAERIGLPLSETRGVANI
                     PGFGIEAMIGPNLTAVGSRRLMEQRGFDVAPFPSSGAEAEVWVARDRLLGVIYLRDRL
                     RLATPEVIADLRKAGDTVALISGDRASAAEMVARAAGIEEVHADLTPNEKLEWIHRWR
                     QAGKIVAMVGDGINDAPSLIAADVGLGMGARGSDAALAQADVILMHDRIENVPAAVRL
                     SRRARTIIRQNLVVSLGTVVVLVAFALAQRIRLSLGVVGHEGSTVLVVLNGLRLLRFK
                     PGRAK"
     gene            1988..2746
                     /locus_tag="VNW28_03575"
     CDS             1988..2746
                     /locus_tag="VNW28_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HXA09035.1"
                     /translation="MQHQIIGNTMPVLEIQLAAEEKIIAVSGELSWMTSTIDLSTTTG
                     VGGGGGLLGAFQRVVGGGSLFMTEYCARGGDGTVAFATKLPGHIVPVEVSPGQTYLIH
                     RHGFLCATPGVELSVGFQQSLGAGIFGGDGFILQKLAGQCQAFVELGGEIVTYDLAPG
                     ETLRVHPGHVGMFEASVRFEITRVKGIRNMLFGGDGIFLAALTGPGKIWLQTLTVANF
                     AHALAPYLPGVKAEVTEVGAGAVIGSVIRGLTKG"
     gene            2990..4150
                     /locus_tag="VNW28_03580"
     CDS             2990..4150
                     /locus_tag="VNW28_03580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXA09036.1"
                     /translation="MFLFAGCNKKSSTTKVINSKHVKIGFIGLTCEAPIYTAYEKGFF
                     KEEGLEPELVKCSWANYKDNLALGSYDITHHLVMYFLKPIEQGLDVRFLGGVHTGCLR
                     VQTGVNSPIHKIQDLKGKRIGVPGMGTPPFVFANRVLGTHGVDPSKDITWKVFPAGEL
                     GLALTKGEVDAVADAEPIGTLLVADGRARNIDGMDEATDEPYAKEYCCEIIANGKWID
                     ANPDTAARATRAILKGAVWVQQNPKAAAQLAVDKKYLASTVELNTIAIGRLKYIPSVT
                     RAEQTLYTAAKEMRVAKMLSPGTDTDALAKRAFQHLDGVTDEWIKTVQVEKVPGGQID
                     PNEDIRMYAELIQKDTEDSCCRRKLFDQPDQDAPNLADPDAPCLQKKTIVRK"
     gene            4241..>4474
                     /locus_tag="VNW28_03585"
     CDS             4241..>4474
                     /locus_tag="VNW28_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09037.1"
                     /translation="MALKSRPQTLLAVPVAAAIGLAVHYFVPKDEPLYPTHFYPIFLS
                     VMFGLGLAGALAQISFPRLRRWMRKMCPILGAVV"
BASE COUNT          865 a         1324 c         1416 g          869 t
ORIGIN      
        1 agtgtatttt cctctccgac tatcgatcgt cttccgatga caccgcgccc gcgctttacc
       61 tggacgcagc ccaaccaccg tgtgctgctc ctcttcgcgg tgatctgcgg gctgagcacg
      121 ttcagcgcat tcttgctgca acgactcggc gccccggccc tcggcgcgat tccactcttt
      181 atcgtcgcct acttgagcgg cggttggttc gcctcgatcc aactgagcgg cgagctgcgg
      241 cgcggcaagt gggacatcaa cctgctcatg ctcgtcgtcg cgctcggcgc ggccagcgtc
      301 ggcgcttggg cggaaggcgg cacgctgctc tttctcttcg cgctcagcaa cgcgctcgaa
      361 cgctttgcca atcatcggac cgagcagacg atcagctcgc tcctcaagtc cgcgccggag
      421 accgcctggc ggcgggagaa cggacggtgg attgaagtcg cggcagattc gttaggcatc
      481 ggtgatgagg tcctggtcaa accgggggaa ctcttcccgg tcgatggcga ggtaatcgaa
      541 ggcgcgacct cggtcgatga gtccgccctg accggcgaag cgctcccggt ttcgaagcgg
      601 gccggcgatc cagtgagcgg cggcacgatg aacctggaag ggcaggcagt ccttcgggtg
      661 cagcgtttgc cgagcgaaag cgcggtgcaa cgcatcatcg acctgatcga gagcgcgcgc
      721 gaacagaagg cgccggcgca acgtttcacc gacacgttta cgcgctatta cacggtcgtg
      781 gtcctgaccg gcagtgttgt ttttctcggc tggcttttgc tttgggtgcg cgagccattc
      841 gcgcaagccg tttaccggat gatgacgctc cttgtcgtct cctcgccctg cgctctcgtt
      901 ctttcgattc cgtccgccat cctggtcgcg attgcggccg gcgcgcgcca gggcattctt
      961 ttccgcggcg gggtggcggt cgagacgctc gccggggtta atcattttgc cttcgacaag
     1021 accggcaccc taacgacggg cgagctggaa gtcgcgcgga tcgagacgac cggggaaatc
     1081 tcgcgcgaag aagcgctcgc cgtcgccgcg gcggtggccc agaattccac ccatccgctt
     1141 tcccgcgccg tcgtggcgga agccgaacgc atcggtttgc cgctgagcga aacgcggggc
     1201 gtcgccaata tccccggatt cggaatcgaa gcgatgatcg gtccaaacct caccgcggtc
     1261 ggaagccggc gcctgatgga gcagcgcgga ttcgacgtcg cgccgttccc ctcgagcggc
     1321 gcggaggccg aggtttgggt ggcccgcgac cggctgctcg gcgtcattta cctgcgcgac
     1381 cggctccgcc tcgccacccc ggaggttatc gcggacctgc ggaaagcagg cgacacggtc
     1441 gccctgattt ccggcgatcg cgcctcggcc gccgaaatgg tggcgcgggc ggcggggatc
     1501 gaggaagtac acgccgatct tacgcctaac gaaaagctgg agtggattca ccgctggcgg
     1561 caggcgggaa aaatagtcgc gatggtgggc gacgggatca acgacgcccc cagcctgatc
     1621 gcggccgacg tcggcctcgg gatgggcgcg cgcggctcgg atgccgccct cgcgcaagcc
     1681 gacgtgatcc tgatgcacga ccggatcgag aacgtgccgg cggccgtccg cctgagtcga
     1741 cgagcccgca ccattatccg gcagaatctc gtcgtgtcgt taggcacggt cgtcgtcctg
     1801 gtagctttcg cgcttgcgca aaggatcagg ctttcgctcg gggtcgtcgg ccacgaaggg
     1861 agcacggtgc tggtggtgct caacgggctg cgactgcttc ggttcaagcc ggggcgcgct
     1921 aagtaaatat tgagcgcgcg tgccgcgcgc gttgcagcca gagccggtcg gagggatagg
     1981 tctgctgatg caacatcaaa tcattggcaa cacgatgccg gttctggaaa tccagctcgc
     2041 ggccgaagag aaaatcatcg ctgtttcggg cgagctttcc tggatgacga gcacgatcga
     2101 cctgagcacg acgaccgggg ttggcggcgg gggcggatta ctcggcgcct tccagcgcgt
     2161 ggtcggcggc ggctcgcttt tcatgacgga atattgcgcg cggggcggcg acgggacggt
     2221 cgccttcgca acgaagttgc cggggcacat cgtgccggtc gaggtttcgc cggggcagac
     2281 ctacctgatc catcgccacg gctttctttg cgcgacgccg ggggtagaat tgagtgtcgg
     2341 tttccagcag tcgttaggcg cgggcatctt cggcggcgac ggattcatcc tgcaaaagct
     2401 cgccggacaa tgccaggcgt tcgtcgaact cggcggcgag atcgtgacct acgatctcgc
     2461 gcccggggaa acgcttcggg tccacccggg ccacgtcggg atgttcgaag cgagtgtccg
     2521 cttcgagatc acgcgggtca aaggcattcg gaacatgctc ttcggcggtg atggaatttt
     2581 cctcgccgct ctcaccggac ccggaaagat ttggctccag acgctcacgg tggccaattt
     2641 cgcccacgcc ctggcgccgt acctgcccgg cgtcaaagcc gaggtgacgg aagtcggcgc
     2701 gggcgcggtg atcggctcgg tgatccgcgg cctaacgaag ggctaaccaa ctccgcgcgg
     2761 ctatttgtta atgatcttgg ggattttcgc gcgggccaat tcgctgatcg cactgttggt
     2821 gctggatcca tttcctcgcg aagcccgccc agaaacttgc cggagtgaga ggaactccaa
     2881 attctaaaga ttttgcttcc atcggcgacg cttattgatc taaggtctgg agttcgaaca
     2941 ttacaattcc atgacaactt caagactttt cagagtcgtt ctgtgcaccg tgttcctctt
     3001 cgcaggctgc aacaagaagt cgagcacgac caaagtcatc aattcgaagc atgttaagat
     3061 cggctttatc ggattgacct gcgaagcgcc gatttatacg gcgtatgaga aagggttctt
     3121 caaagaagaa ggactggagc ctgaattagt gaaatgcagc tgggcgaatt acaaggataa
     3181 tctcgcgctc ggcagctacg acatcactca ccatctcgtg atgtatttcc tcaaaccaat
     3241 cgagcaaggc ttggatgtga ggttccttgg gggagtgcac accggatgcc tgcgagtcca
     3301 aaccggcgtg aacagtccga ttcacaaaat ccaggacttg aagggaaaac ggattggagt
     3361 cccgggaatg ggcacgcctc cgttcgtctt tgccaaccgc gttctcggaa cccacggcgt
     3421 cgatcccagc aaggatatta cctggaaggt ttttccggcc ggagaactgg gattggcgtt
     3481 gaccaaggga gaggtggatg ccgtggccga cgccgagccg attgggaccc tgttggtcgc
     3541 ggacggcagg gcgagaaaca tcgacggaat ggacgaggcg accgacgaac cttacgcgaa
     3601 agagtattgc tgcgaaatca tcgcgaatgg caaatggatc gatgccaacc ccgacaccgc
     3661 ggcccgagca acccgggcga tcttgaaagg ggcggtatgg gtgcagcaaa acccgaaggc
     3721 tgcggcacag ctggcggtgg acaaaaaata cctcgcttca acggtcgaac taaacaccat
     3781 cgcgatcggc cgcttgaaat acattccgtc cgtcactcgg gctgagcaaa cactctacac
     3841 cgcggccaaa gaaatgaggg tggccaagat gctttcgcct ggcaccgata ctgacgcatt
     3901 ggcgaaacgc gccttccagc atcttgatgg cgtaaccgac gagtggatta agaccgtcca
     3961 agtggaaaaa gtcccgggcg gccagatcga ccctaacgaa gatatccgca tgtatgcaga
     4021 gttgattcag aaagacaccg aagattcctg ctgccgaaga aaattgttcg atcagccgga
     4081 ccaggacgcc ccgaatctgg ctgatcctga cgccccgtgc ctccagaaga agacgatcgt
     4141 ccgtaaatga atgaatagtg ccgttgaggg agtcggaact ggagcaccgg tcatcacaat
     4201 gacgcggtcc gccgctggtg aggccgcgat tccagctcca atggcgctga aatcgcggcc
     4261 gcagacactc ctggctgttc cagtcgcagc ggcgatcggt ctcgcggtgc actatttcgt
     4321 gccgaaagac gaaccgctct atcccaccca tttctatccg atattcctga gcgtcatgtt
     4381 cggcttggga ttagcgggcg cgctggcgca gatttctttt ccgcggcttc gtcgctggat
     4441 gcgcaagatg tgtccgattc tcggtgcggt tgtc
//
LOCUS       DATMNS010000097         6044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3289036, whole genome shotgun sequence.
ACCESSION   DATMNS010000097 DATMNS010000000
VERSION     DATMNS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3289036"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VNW28_03590"
     CDS             <1..302
                     /locus_tag="VNW28_03590"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXA09038.1"
                     /translation="PRFSPDALKANQAMVDLLGKIAAAKKATPAQIALAWLLAQKPWI
                     VPIPGTTKLHRLEENLGAVNVELTPDDLREIDEAASEIDVQGARYPEKLEAMTGR"
     gene            404..2209
                     /locus_tag="VNW28_03595"
     CDS             404..2209
                     /locus_tag="VNW28_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXA09039.1"
                     /translation="MALSETDIQRQKEIKQAEELLFSGHQELGFAKGLFLGNFVADWA
                     MPYPQLTDAEQADVDRAVTELRQFLDEHLDPEEIDRQADIPRHVIDGLGRVGVLGMTA
                     PKEVGGRGFSQMQYCKVLEEIGARCASTAVFTNAHHSIGIRALLLFGTKEQQEKWLPK
                     LMHGEQLAAFALTEQEAGSDAANVQMRATPSEDGSHYILNGEKRYITNASIAQVLTVM
                     ARTPVPGKDKDAITAFLVTPDMEGFEMIEPRMPKLGIRGTATGRFRLNNVRVPKENIL
                     GPLGKGLRVALTVLDFGRTTFGACCTGGAKTCLRLAIEHANKRKQFNKTLGNFDLVKK
                     KIARMAADVYAMEAMTQVTASLIDRGLEDYMVETAMLKVFTTERLWDAVNDCFQIHGG
                     SAYFDDSPLGRMLRDARINQIGEGSNEVLTSFIALVGMRGPGMEFKEIYDTMLKPWRP
                     EGAKAAWGAGLKRLSAAVKVPEVPVRNDRLKSYAGQLARLVWRFNFAVDKALIAYREP
                     VMEMQLVQERIANAAMELFAATCVLSRWDSELSVVGRNGRAATDHVAADLFVRRALRQ
                     IRQFLRGLGDNDDAALLATADTVLGKSGTPKQNGA"
     gene            2488..4005
                     /locus_tag="VNW28_03600"
     CDS             2488..4005
                     /locus_tag="VNW28_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HXA09040.1"
                     /translation="MDPVPALDLQGQRVLIFIVAYNAETTIEKVLNRIPASLQQPNVE
                     VLIIDDSSQDDTFLNGLRYQQAHSAFKITVLRTPENQGYGGNQKLGYRYAIENHFDIV
                     ALVHGDGQYAPEKLPALLAPLLAGEADAVFGSRMFDKSAARAGGMPFYKWIGNQILTG
                     FQNRMLGTRLSEFHSGYRLYSTRALAQIPFEKNTHDFHFDTEIIIQFVLQKLRIVELP
                     IPTFYGDEICRVNGLKYASDVFRATIKGRLCGMHLFYDRKFAVGRETAAHSDKLGFPS
                     SQSFALERCTAGRNILQLGIGHAHVADELQRRGCRVTPIDFAANELTSHSETVCLASR
                     PQLPQDISVYDQILLMDVVEHLHDPEGFMDELRHRTADRRPEVIVTAANVAFFITRIM
                     LALGQFNYSRKGILGLGHRRLFTFRSLHALLEQAGYEVLEARGVPAPYPLALGSNRRS
                     RILLGLNQWLIRLSKGLFGYQICLRARPLPHSHHLLKETISGSAGLRAEVLARVA"
     gene            4106..5440
                     /locus_tag="VNW28_03605"
     CDS             4106..5440
                     /locus_tag="VNW28_03605"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXA09041.1"
                     /translation="MPVLRAKPQVARLPPTALRFDRTPPPRYREIEKCRICGNENLVP
                     VLSLGEQFLTGVFPRGLHATLSKGPLELVACAATENEDACGLVQLRQSYELGELYGEN
                     YGYRSSLNASMVRHLKSKVDQLIERYPLQSGDLVLDIGSNDGTLLSFYPEKNVTVVGI
                     DPTGRKFASYYRKHITLLPDFFSADLFGKMFGSRRARIVTSISMFYDLESPLRFMEEV
                     ASILDEEGVWHFEMSYMPSMVRTMGYDTICHEHLEYYSLHQIKWMTDRSGLKILDVAF
                     NDINGGSFAVTVARRSSKHARDTTQIKRLLREEKTQGLSTLAQQRDFRKAVEAHKKDL
                     LSVLHILKAEKRTVLGYGASTKGNVLLQHCGISRDLLPAIAEVNQDKFGCFTPGTNIP
                     IISEAEAHAMKPDYLFVLPWHFRANIIEREQAFLNRGGRLLFPLPAIEIVGG"
     gene            5444..>6044
                     /locus_tag="VNW28_03610"
     CDS             5444..>6044
                     /locus_tag="VNW28_03610"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXA09042.1"
                     /translation="MKRNALIVGASGQDGRLLTRQLLDRGYAVRGWTRTKPAPQIECE
                     CAQIDLLDSLAIEKELQNLSPDEIYYLAAFHHSTEDSLALTPTELTQRSFEGQVAGLR
                     HVLEAIEAVAPRTRLFYAASSHVFGEAVTASQNEQTPFAPTSVYGISKAAGVACCRHF
                     REKGIFAATGILFNHESALRKTSFLSQKVAQGVLRARREP"
BASE COUNT         1229 a         1877 c         1785 g         1153 t
ORIGIN      
        1 tcccgcgctt ctcgccggat gcattgaagg cgaaccaggc gatggtggac ttgttaggca
       61 aaatcgccgc ggcgaaaaag gcgacgccgg cgcagatcgc gctcgcatgg ctcctggcgc
      121 aaaagccgtg gatcgtgccg atcccgggca cgacgaaact gcatcgactg gaagagaatc
      181 tcggcgcggt aaatgtcgag ctgacgccgg atgatttgcg cgagatcgat gaagcggcgt
      241 cggagatcga cgtgcaaggg gcgcgttatc ccgagaagct cgaagcgatg acgggacggt
      301 gagttattcg ccattgagaa tagccggcgg ggtggacaat ccggcgccct tggctacagt
      361 tctggccccg ctcgcgcgcc gacgggcttc acctttctca cctatggcac tcagcgagac
      421 cgacattcaa cgtcagaaag aaattaaaca ggccgaggaa cttctcttca gcggccacca
      481 ggagcttggt tttgccaaag gattgttcct cggaaatttc gtcgccgact gggcgatgcc
      541 ctatccgcag ctgaccgacg ctgagcaggc ggacgtcgat cgcgcagtca ccgagttgcg
      601 ccagtttttg gatgaacacc tcgatcccga ggagatcgac cgccaggccg acatcccccg
      661 ccacgtgatc gacggactcg gtcgggtcgg cgtgctcggg atgacggcgc cgaaggaagt
      721 gggcgggcgc ggattttcgc agatgcagta ttgcaaagtg ctcgaggaaa tcggcgcgcg
      781 ctgcgcttcg accgcggtgt tcacgaacgc gcaccactcg atcggtatcc gggcgctgct
      841 ccttttcggc acgaaagagc agcaggaaaa atggctgccg aaattgatgc acggcgagca
      901 gctcgccgcc tttgccctca ccgagcagga agccgggtcc gacgcagcca acgtgcaaat
      961 gcgcgccacg ccgagcgaag acggctcgca ttacattctc aatggcgaaa agcgctacat
     1021 caccaacgcc tcgatcgccc aggtcctgac cgtgatggcg cgcaccccgg ttcccggcaa
     1081 ggataaggac gcgatcaccg cgtttctcgt cacgcccgac atggagggct tcgagatgat
     1141 cgagccgcgc atgccgaagc tcgggattcg cggcacggcg acgggccgtt tccgactcaa
     1201 caacgtgcgc gtgccgaagg aaaatattct cggcccgtta ggcaaaggcc tgcgcgttgc
     1261 gctcaccgtt ctggatttcg gccggaccac cttcggcgcc tgctgcacgg gcggagccaa
     1321 gacctgcctg cggctggcga tcgagcacgc gaacaagcgc aaacagttca acaaaacgct
     1381 gggcaatttc gatctggtga agaaaaaaat cgcgcgcatg gcggccgacg tttacgcgat
     1441 ggaagcgatg acgcaggtga ccgcctcgct gatcgatcgc ggcctcgagg attacatggt
     1501 cgagaccgcc atgctcaagg tcttcacgac cgagcggctc tgggacgcag tcaatgattg
     1561 cttccagatc catggcggct ccgcctattt cgacgactcc ccgctggggc ggatgctgcg
     1621 cgacgcgcgg atcaaccaga tcggcgaagg gagcaacgaa gtgctcacct ctttcatcgc
     1681 actcgtggga atgcgcggac ccgggatgga gttcaaggaa atctacgaca ccatgctcaa
     1741 gccgtggcgt ccggaaggag cgaaagccgc ctggggcgcc ggcctgaaac gactcagcgc
     1801 cgcagtcaag gtgccggagg tgccggtgcg caacgatcgg ctgaaatcgt acgccggtca
     1861 gttggcgcgc ctggtttggc gtttcaactt cgcggtcgac aaggcactaa tcgcctaccg
     1921 cgaaccggtg atggagatgc aactggtgca ggaacgcatt gccaacgcgg cgatggaatt
     1981 gtttgccgcg acctgcgtgc tgagccgttg ggactccgag ctgtcggtcg tgggccgcaa
     2041 cggccgggcc gcgaccgatc atgtcgccgc ggaccttttc gttaggcgcg cgcttcgaca
     2101 aatccggcaa ttcctgcgcg ggttgggcga taacgatgat gcggcgctgc tcgccactgc
     2161 cgataccgtg ttgggcaaga gcggcacacc gaagcaaaac ggcgcctagt ggtgtgtatc
     2221 ccttgaatgg gtagtaatgc gagcgccctt tccggggagc gcacgcgccc tcgcgtgctg
     2281 gctgcggcgc ctcgccgcaa cgaactttcc aagggagcat cgccgaggag aaaaagctcg
     2341 cgatgacgag ggcgtcatcg ccagcacgcg aggcgcgtgc ggtccccaga cggcctgttg
     2401 tcgcgcttta ctaatgatcg ataagtccat tagcaccgca ctcgctcccg gagtgcaact
     2461 ggcccgtttt tagctccgac gtggggcatg gaccccgttc cagcgctcga cttgcagggc
     2521 cagcgcgttt tgattttcat cgtcgcctac aacgctgaga cgacgatcga gaaggtgctg
     2581 aaccgcatcc cggcctcgct ccagcagccg aacgtcgagg tgctgatcat tgacgactct
     2641 tcgcaggacg acacttttct caacggcctg cgttaccagc aggctcattc cgccttcaag
     2701 atcacggtcc tgcgcacgcc ggaaaatcag ggctacggcg gcaaccaaaa actcggctac
     2761 cgctacgcga tcgagaacca cttcgacatc gtggcgctcg tgcatggcga cggccagtat
     2821 gcgccggaaa agctgccggc cttactcgca ccgctccttg caggggaagc ggacgcggtt
     2881 ttcggctcgc gcatgttcga caaaagcgcg gcgcgcgccg gcggcatgcc gttctacaaa
     2941 tggataggca accagatttt gaccggtttc cagaaccgca tgctgggcac acggctctcg
     3001 gaattccatt ccggttaccg cctttattcg acccgggcgc tggcccagat cccattcgaa
     3061 aaaaacaccc acgatttcca cttcgacacc gagatcatca tccagtttgt cttgcaaaag
     3121 ctccgcatcg ttgagttgcc gatcccgacc ttttacgggg acgaaatctg ccgcgttaac
     3181 ggtttgaagt acgcgtccga cgtttttcgc gcgacgatca aagggcgttt gtgcggcatg
     3241 cacctgtttt acgatcgcaa gtttgccgtc ggtcgcgaaa ccgcggccca ttccgacaaa
     3301 ctcggttttc catcgtcgca atcgttcgcg ctcgagcgct gcactgccgg ccgcaacatt
     3361 ctccaactcg gcatcggaca cgcacatgtg gcggacgaat tgcagcgccg cggttgccgc
     3421 gtcacgccaa tcgatttcgc ggctaacgaa ttgacttcgc actcagaaac cgtctgcctg
     3481 gcgtcccgcc cccagctgcc gcaggacatc tcggtctacg atcaaattct cctcatggac
     3541 gtggtcgagc acttgcatga cccggaaggt ttcatggacg aattgcgcca ccgcacggcc
     3601 gaccgccggc ccgaagtaat cgtcactgcg gccaacgtcg cctttttcat cacccgaatc
     3661 atgctcgcgc tgggtcagtt caactacagc cggaagggaa ttctcggcct cggtcatcgc
     3721 cggctcttta cctttcggtc gttgcacgcg ttgctcgagc aggcgggtta cgaggtgctc
     3781 gaggcgcgcg gggtcccggc gccttatcct cttgcgctcg gctcaaatcg ccgcagccgg
     3841 atcctgctcg gactcaacca atggctgatc cgattgagca agggtctgtt tggttaccag
     3901 atttgcctgc gcgcgcgtcc cctcccgcat tcgcatcatc tcctgaagga gacgatctcg
     3961 ggcagcgccg ggctgcgggc cgaagtgctg gctcgtgtcg cctaacgatc tccgaaaata
     4021 aatctcccgc aattgcctcg cccaccgcgg caaaagaccc ggcttgtcgc ggggcaatgg
     4081 cacggacagt gctggaaaaa gggccatgcc agtccttcgc gcaaagccgc aggttgctcg
     4141 tcttccgccg acagcgttgc gcttcgatcg caccccgccg ccgcgctacc gcgaaatcga
     4201 aaaatgccgg atctgcggaa atgagaacct tgtgccggtt ctttcccttg gtgaacaatt
     4261 tctgaccggg gtttttccgc gcggactcca cgccacgctg agcaagggac cgctcgaact
     4321 ggtcgcctgc gccgcgaccg aaaacgagga cgcgtgtgga ttggtgcagt tgcgtcagtc
     4381 ctacgaactg ggcgagttgt acggcgaaaa ttacggttac cggtcctcgc tcaacgcttc
     4441 gatggtgcga cacctcaaga gcaaggtaga ccagctgatc gagcgctacc cgctgcagtc
     4501 aggcgacctc gtgctcgata tcggcagcaa cgacgggacg ctcctttcat tttatcccga
     4561 gaaaaatgtg acggtggtcg ggatcgatcc gacgggacga aaattcgctt cctactatcg
     4621 gaaacacatc acgttgctcc cggatttctt ttccgccgat ctcttcggga aaatgttcgg
     4681 ctcgcgccgg gcccgaatcg tgacctcgat ctcgatgttc tacgatctcg agagcccgtt
     4741 gcggttcatg gaggaggtcg cctcgatcct cgacgaggaa ggcgtctggc atttcgaaat
     4801 gagctacatg ccctcgatgg tgcgcacgat gggttacgac accatctgtc acgaacatct
     4861 cgagtactac tcgctgcatc agatcaaatg gatgaccgac cggagcggtc tcaagattct
     4921 ggacgttgcc ttcaacgata taaacggcgg aagcttcgct gtgaccgtgg cgcgccggtc
     4981 ttccaagcac gccagggaca cgacccagat caaacgcctg ctgcgggaag aaaagacgca
     5041 agggctgagc accctcgcgc agcaacgcga cttcaggaag gcggtcgagg cccacaagaa
     5101 ggatctcctc tccgtgctgc acatcttaaa ggcggagaaa cgcaccgtcc tcggttacgg
     5161 cgcttcgacc aaaggaaacg tccttttgca gcactgcggc atctcccgcg acctgctccc
     5221 ggcaattgcg gaagtgaacc aggataaatt cggctgcttc acgcccggca ccaatatccc
     5281 aattatttcc gaagctgaag cgcacgccat gaagcccgat taccttttcg tccttccctg
     5341 gcatttccgc gccaatatca tcgagcgcga acaggctttt ctgaaccggg gcggaaggtt
     5401 gcttttccct ctgcccgcaa tcgaaatcgt cggaggttaa cccttgaagc gaaacgcgct
     5461 catcgtcggc gcgagcggac aggatggccg tctgttgacc cggcaacttc tggatcgcgg
     5521 ctacgccgtt cgcggctgga cccgaaccaa accagcgccg cagatagaat gcgaatgcgc
     5581 ccaaatcgac ctcctcgatt ccctcgcgat cgagaaggaa cttcagaacc tgtcacccga
     5641 tgaaatctac tatctggcgg cgtttcatca ttccaccgaa gactctctcg cgctcacacc
     5701 gacagagctg acgcaacgca gtttcgaggg gcaggtcgcc ggcctgcgcc acgtgctcga
     5761 ggcgatcgag gcagtggcgc cccgaacacg acttttctat gcggcttctt cgcacgtttt
     5821 tggcgaagcc gtcacggcat cgcagaacga gcagacgccg ttcgcgccga catccgttta
     5881 cggcatctcg aaggcggcgg gcgtcgcgtg ttgccgtcac ttccgcgaaa aaggaatctt
     5941 cgccgcgacc ggcattctgt ttaatcacga atcggcgcta cgcaaaacgt cgttcctctc
     6001 gcagaaagtt gcgcagggcg tcctgcgggc gcggcgcgaa ccgg
//
LOCUS       DATMNS010000098         3763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4007346, whole genome shotgun sequence.
ACCESSION   DATMNS010000098 DATMNS010000000
VERSION     DATMNS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4007346"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(116..751)
                     /locus_tag="VNW28_03615"
     CDS             complement(116..751)
                     /locus_tag="VNW28_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HXA09043.1"
                     /translation="MKGRLTVAMALALLATVRVGAQQAAQAAEAPPKPQASPARAATD
                     ANGQPITTQIYSDETSFNTEKRIGFFTGHVRVFDPRFNIQSDKLTVFIHKEEGEGLEK
                     AIAEGNVGVVRDRPDPKGGPPSKSVGLSEKAVYTSSDGNVELTGSPRVQEGLDTHIAT
                     SPATVMVLSQDGHLTTHGPSRTEIRQDANAKGSPSPTPTPKPVRKKRVKKV"
     gene            complement(748..1293)
                     /gene="lptC"
                     /locus_tag="VNW28_03620"
     CDS             complement(748..1293)
                     /gene="lptC"
                     /locus_tag="VNW28_03620"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HXA09044.1"
                     /translation="MLPCRSGSRLFRGTRLSLLLLLVGSCAASAGDRKQRKPTPTPAP
                     ETQKEDLGLKNIPLTVGHEAKGLVLPNYDADGHLLGRFEAATAARLDENHVHFTNVKM
                     LTFDAKEQPDFNVAMSDAILNLETRVIASKHRTKIKRTDFEIAGDAMTFSTVTHQGTL
                     TGNVHMTIFNQKEIAGGAAKP"
     gene            complement(1277..2095)
                     /gene="kdsA"
                     /locus_tag="VNW28_03625"
     CDS             complement(1277..2095)
                     /gene="kdsA"
                     /locus_tag="VNW28_03625"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HXA09045.1"
                     /translation="MKPKPLTIGPVTLGGKTPAFILGPCVIESEKFVRRMARKIARIC
                     AAEDLPFIFKASYDKANRTSVRSYRGMGAREGCELLAGIGREIGLPVTTDVHSPNEAE
                     IAAEFVDVLQIPAFLCRQTDLIQAAAETGRVVNVKKGQFLAPWDVRNIAEKLEAFGAH
                     KYFFTERGTTFGYNNLVADMRSLAWLRAAGHHVIFDATHSVQRPGGAGDFTSGDAALA
                     PVLARAALAAGCDGIFMEVHENPSRALSDGPNQVPLKDLPKHLRMLKAIHAAVS"
     gene            2206..3756
                     /locus_tag="VNW28_03630"
     CDS             2206..3756
                     /locus_tag="VNW28_03630"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HXA09046.1"
                     /translation="MKRDPSLIGLPPELSVEEAAARFDLLQQRLVPLWSSIESFNQDE
                     QTIVVVPSISLEMAVSGFEIQGYEERFLFLLLLLAQPRARLTYVTSQAIHPSVVEYYL
                     GLLPGVIRSHALERLTLLCPYDDSARPLSLKLLERPRLLERVEAGIRDKERAHLVCYN
                     TTYLERNLALRLGIPLYGADPRHFSFGTKTGCRQLFARAGINHPLGYESLGSMDEVVS
                     ALARMQAERSSILQAVVKLNEGVSGEGNALVDLSNLGSPLNGAIGERVRAMRFEKPDI
                     RFEEYAEKFRQGGGVVEERVGVGAGEVRSPSVQLRVTPLGKVELLSTHDQMLGGPGGQ
                     SYLGCIFPADPAYAGAITRDAAKVGELLRDAGVIGRFALDFVVTRAPGTADWETYAIE
                     INLRKGGTTHPFLTLQFLTSGSYDAEQAVFTAPNGRKKFLVASDHLDSPRYRGYTPDD
                     LFDLVVRRGLHFGQTRQTGVVFHMLSALGSTGRLGLTAVGNSPEEARVIFDQAKSALD
                     EEASFDEL"
BASE COUNT          657 a         1187 c         1163 g          756 t
ORIGIN      
        1 gcccggccgc catagatctt gaccagatca tgggtcagca ggacttcctc gtgttcgtta
       61 ggctgaagcg ccatccccgc ggccggggcg gcgagggacg gctcaggcga agtgatcata
      121 ccttttttac ccgtttcttc cggaccggct ttggggtcgg tgtcggggaa ggcgaccctt
      181 tcgcattcgc gtcctgccgg atttcggtgc ggctcgggcc gtgcgtggtg agatggccgt
      241 cctggctcag caccatgacc gtggcgggac tcgtcgcgat gtgcgtatcc aaaccttcct
      301 ggacccgcgg gctgccggtc aactcgacgt ttccatccga tgaagtgtag accgcctttt
      361 ccgacagccc gaccgatttc gagggcgggc cccctttcgg atcgggccga tcgcggacca
      421 caccgacgtt gccttcggcg atcgctttct cgaggccttc gccttcctcc ttgtgaataa
      481 agaccgtcag cttgtccgat tgaatgttaa agcgcggatc gaaaacccgg acatggccgg
      541 tgaagaaacc aatgcgtttc tcggtattga acgatgtttc gtccgagtag atctgggtcg
      601 tgatcggctg gccgtttgca tccgtcgcgg cgcgcgccgg tgaagcctgg ggtttcggag
      661 gggcctccgc ggcctgggcc gcctgctgcg cgccgacccg aacggtcgcg agcagcgcga
      721 gcgccattgc cacggtcaac ctccctttca tggtttcgcc gccccgccgg caatttcctt
      781 ctggttgaaa attgtcatgt gcacattgcc ggtgagcgtt ccctggtggg tcacagtgct
      841 gaacgtcatg gcatcgccgg cgatctcgaa atcggtgcgc ttgatcttgg tccggtgctt
      901 ggaagcgatc acccgcgttt cgagattgag gatggcgtcg ctcatagcga cgttgaaatc
      961 gggctgctcc tttgcatcga acgtcagcat cttcacgttg gtaaagtgaa catgattttc
     1021 atcgaggcgc gcggccgtgg cggcttcgaa acggccgagc agatgcccgt cggcatcgta
     1081 gtttggcaga acgaggcctt tcgcttcgtg cccaacggtg agcgggatgt ttttcaggcc
     1141 caggtcctcc ttttgcgttt cgggcgcggg cgtcggggtc ggcttccgct gcttgcgatc
     1201 gccggcggag gcagcgcagc tgcccacgag caacaagaga agactcaggc gcgtcccgcg
     1261 gaagaggcgg gacccgctac gacacggcag catgaatagc ttttaacatg cgcagatgct
     1321 tcggcaaatc ctttagcgga acttgattcg gcccatcgga gagggcgcgc gacgggtttt
     1381 cgtgcacctc catgaaaata ccatcgcacc cggcggccag cgcagcccgc gcgagcacgg
     1441 gcgcgagcgc ggcgtccccg gacgtgaagt cgcccgcgcc cccggggcgc tgcaccgagt
     1501 gcgtcgcatc gaaaatcacg tgatggccgg cggcacgcag ccaggccagc gagcgcatgt
     1561 cggccacgag attgttatag ccgaaggtcg tcccgcgctc ggtgaagaaa tacttatgcg
     1621 cgccaaaggc ctcgagcttc tctgcgatat tgcgcacatc ccacggcgca aggaattgtc
     1681 ccttcttcac attcaccacc cggccggttt ccgccgccgc ctgaatcagg tccgtctgcc
     1741 ggcagaggaa tgccgggatt tgcaagacgt cgacgaactc cgccgcgatt tccgcctcgt
     1801 taggcgaatg gacatcggtc gtcactggca gcccgatttc gcggccgatt ccggccagca
     1861 attcgcaacc ttcgcgggcc cccatgccgc gataggagcg caccgaggtg cggttcgcct
     1921 tgtcgtagga agccttgaag atgaagggca gatcttccgc ggcgcaaatc cgtgcaatct
     1981 tgcgcgccat tcgcctaacg aatttctcgg actcgatcac gcacggcccg agaatgaagg
     2041 cgggcgtctt gccacccagc gtcactgggc caatcgtcag gggcttcggc ttcacgaggc
     2101 agcattctcg tcaaaagccc gggcaagtcc aaggcgattc gagccgggcg aaattctgct
     2161 cgcctcgcgc gctcgtccct tgcagaatcc acccttttgt cgcctatgaa acgagatccc
     2221 agtctgatcg gacttccccc tgagctttcg gttgaggaag ccgcggcccg gttcgatctc
     2281 ttgcaacaga gactcgtgcc gctctggagt tcgatcgaat cctttaacca ggacgaacag
     2341 acgatcgtcg tcgttccctc gatctcgctc gagatggcgg tgagcggctt cgaaattcag
     2401 ggttacgaag agcgcttcct ttttctgttg ctcttgctcg cgcaaccacg ggcacggctc
     2461 acctacgtca cttcccaggc cattcacccg agcgtcgtcg agtattacct cggtctgctc
     2521 ccgggcgtaa tccgcagtca tgcgctcgaa cgtctgaccc tgctctgccc ctacgatgat
     2581 tccgcccggc cgctcagcct gaaactgctc gaaagaccgc gactcctcga gcgggtcgaa
     2641 gcgggcatcc gggacaagga acgcgcgcat ctcgtttgct ataacacgac ctacctcgag
     2701 cgaaacctcg cgctccgcct cgggatccca ctttacggcg ccgacccacg acatttctcg
     2761 tttgggacca agaccggttg ccggcaactg tttgcccggg cagggatcaa tcatccactc
     2821 gggtacgaga gccttggcag catggacgag gtcgtctccg cgctcgctcg gatgcaggcc
     2881 gagcggtctt ccatcttgca ggcggtcgtg aaactgaatg agggcgtttc cggcgaaggc
     2941 aatgcgctgg tcgacctctc gaacctgggc tcgcccctga acggagcgat tggcgagcgg
     3001 gtgcgcgcaa tgcgcttcga gaaaccggat attcgcttcg aggagtacgc cgaaaaattt
     3061 cgccagggcg gaggagtggt ggaggaacgg gtcggggttg gcgccggcga ggtgcgcagc
     3121 ccgagtgtgc aactgcgggt cacgccgtta ggcaaagtgg agcttctctc gactcacgat
     3181 caaatgctcg gcggcccggg cggccaaagc tacctcggct gcatttttcc cgcggacccg
     3241 gcttacgccg gcgcgatcac gcgggacgcg gccaaggtgg gcgagctgct gcgcgacgcc
     3301 ggggtgatcg gccgcttcgc gctcgacttt gtcgtgacgc gtgcgcccgg caccgcggat
     3361 tgggagacct acgcgatcga gatcaacctg cgcaaaggcg gtaccaccca tccgtttctc
     3421 accctgcaat ttctgaccag cggttcctac gatgcggagc aggcggtctt cactgcgcct
     3481 aacgggcgca agaaatttct cgtcgcgagc gatcatctcg attcgccgcg ctatcgcggt
     3541 tatacaccgg acgatctctt tgatctcgtc gtccgccgcg ggctgcattt tggtcaaacc
     3601 cggcagaccg gcgtcgtctt tcacatgctg agcgcgctcg gcagcacggg ccgcctcggc
     3661 ctgaccgcgg tcggcaattc gcccgaggaa gcgcgcgtca ttttcgacca ggccaaaagc
     3721 gcgctggatg aggaagccag tttcgacgag ctctagccgc cgc
//
LOCUS       DATMNS010000099         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4369027, whole genome shotgun sequence.
ACCESSION   DATMNS010000099 DATMNS010000000
VERSION     DATMNS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4369027"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..1516
                     /gene="nusA"
                     /locus_tag="VNW28_03635"
     CDS             56..1516
                     /gene="nusA"
                     /locus_tag="VNW28_03635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXA09047.1"
                     /translation="MLSREKHIEPEVVISAIEDAMVVAARKFYKTEEDLRGKFNAETG
                     QVDVHSVYTVVEEVTDPKREISLADAKKKNPGAEIGTEILTAKPTDVLGRIAAQTAKQ
                     VILQKVREAERDTIYNEYHTRAGELVTVIVKRAEGPDLIVDMGRTEARLPKREQSKLE
                     TYNIGERLRVVIKAVDRAAKGPQVIVSRADASLVQRLFEMEVPEIYDGTVQIRAAARE
                     AGERTKVAVASRDKDVDPVGACVGMKGMRVQSIIRELRGEKIDIIPYNEETVAFAQKA
                     LSPAKVTRVQITDPENKKLEVIVEDSQLSLAIGKKGQNVRLASKLIGWDIDIKSEEEK
                     RREIEEQMTALTAPTTPLTVLAGVGPKTIEKIEAAGIANLEKLASMTPEQLMEIPGIG
                     EKMVDKIYQAVNRFYEGGDAAATPVEAADESGEEAPAAESATESAVEASDDSAADAAS
                     ETPTSVPTDSASETNSEAAASSENESPAEQQEEKSS"
     gene            2500..>3053
                     /locus_tag="VNW28_03640"
     CDS             2500..>3053
                     /locus_tag="VNW28_03640"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HXA09048.1"
                     /translation="MLPSPFSEKPSKAEPGKPLYARKPQQRQRPMADKREMEGERKLH
                     PTRQRPGAGGRGVAAAAIAPPEPRAPRDIQLTEGITIRELAEKLDVRAKDLLKTLLDR
                     GVFASINQALDVQTATDLAQSFNGVVSVVSLEEEMVLEVAKEETKENLKPRPPVVTVM
                     GHVDHGKTSLLDAIRQEDVAAGEAG"
BASE COUNT          693 a         1034 c          883 g          443 t
ORIGIN      
        1 ggtcgtggag ttttaggaaa gcgaaatggc gaatctgctc tatcaacaaa tcgagatgtt
       61 gagccgggag aagcacatcg aaccggaggt ggtgatttcc gccatcgaag acgccatggt
      121 cgtggctgcc cggaaatttt acaagaccga agaagacctg cgaggaaaat tcaacgccga
      181 aacaggtcag gttgacgtgc attcggtcta caccgtcgtc gaagaggtga cggacccgaa
      241 gcgcgaaatc agcttggccg atgcgaagaa aaagaatcct ggcgccgaaa tcgggaccga
      301 gattctgacc gccaagccca ccgacgtgct gggccgcatt gccgctcaga ccgcgaagca
      361 ggtgatcctg cagaaggtgc gcgaggccga gcgcgacacc atttacaacg aatatcacac
      421 ccgcgccggt gaactcgtca ccgtcatcgt aaagcgcgcc gaaggccccg acctcatcgt
      481 ggatatgggc cgcaccgaag cccgcttgcc caagcgcgaa cagtccaaac tcgaaaccta
      541 caacatcggc gagcgcttgc gtgtggtgat caaagctgtt gaccgcgccg ccaaaggccc
      601 gcaggtaatc gtttcccgcg ccgacgcttc gctcgtgcag cgcctctttg aaatggaagt
      661 gcccgaaatt tacgacggca ccgtccagat ccgcgccgcg gcgcgcgaag ccggcgaacg
      721 caccaaggtc gccgtcgcca gccgcgacaa agacgtcgac cccgtcggcg cctgcgtcgg
      781 catgaaaggc atgcgcgtgc agtccatcat ccgcgaactc cgcggtgaaa aaatcgacat
      841 tattccctac aacgaagaaa ccgttgcctt cgcgcaaaag gccttgagcc cggcaaaagt
      901 cactcgtgtg cagatcaccg atcccgagaa caaaaagctc gaagtgatcg tcgaagactc
      961 ccagctttct ctcgccatcg gcaaaaaggg acaaaacgtc cgcctggcca gcaaactaat
     1021 cggctgggac atcgacatca agagcgaaga agaaaagcgc cgcgaaatcg aagagcagat
     1081 gaccgctctg accgcgccga cgactcctct gacagtcctc gcgggcgtgg gaccaaaaac
     1141 catcgagaag atcgaagcag ccggcattgc caatctcgaa aaacttgcca gcatgacccc
     1201 ggaacagttg atggagattc ccggcatcgg cgaaaagatg gtcgacaaaa tttatcaggc
     1261 cgtgaatcgc ttctatgaag gcggcgatgc cgcggcaacg cccgtggaag ccgcggatga
     1321 atcaggcgaa gaagcaccag ctgccgagtc ggcgacagaa tcggccgttg aggcatcaga
     1381 cgatagcgcc gccgacgccg cgagtgaaac gccaacttca gtgccaacgg attccgcttc
     1441 ggagactaac tcggaagccg ctgcgtcctc ggagaacgaa tctccggcgg aacagcagga
     1501 agagaaaagt tcctaagaag catgagcgac gcaaatcaag tccgcatcaa tgagctcgcg
     1561 cgcgaactcg aggtcaaggc gaaagccatc atcgaccttt tggccggatt cggcgttacc
     1621 gaaaagaaaa cgcactccag ttccattccc gccgatgtcg ccgaaaaggt taagcagcac
     1681 ctgaagggtg tagcggacgc tgaaagcgcc gctgaagccg atcgcgccgc gaaagaagcc
     1741 gccgcgaaag cggcagcgcg ccggccctct cctccagctg cagctcccgt tgttgcacct
     1801 gccgcgcatc catcggtggc agcgaagcct ccggcccccg cgcccgcggt ggtgcctcct
     1861 ccaaccccgc atgctccagc accagcggcc gtgaagcccg cgccggcccc gactcctgca
     1921 cctgcagcag cacccgcatc agctgctccc gctgcaaaac ccgcagctcc ggctccggcc
     1981 gtcaccaacg cgcccgcagc gccagctgcg gagcgtcccc ctgcccctgc cgcgccagtt
     2041 gctccgaccg cgcagcgccc tgctccacaa cagtctttgc gtccaacgca tcccactacg
     2101 cgtcagccgg gccaacatcc ggcggcacgc cccggcggag ggtcgcctcc gcaatcgcgt
     2161 ccgcagcagc ccggcacgca gccgcaatcc gcgcgctctg gtgctccggc aagtgcccct
     2221 cgcccgcagc aacctcttcg tcccatgccc acaggaaacc gcgggccggt tccgggaact
     2281 tcacccggtg accgcgatcg cagaccaagc ggcccacgcc ccggccagcc gatgcgcccg
     2341 caacaatccg gcggccaggg tggccgtcct tttacaccgc gtccatcagg acccggaggt
     2401 ccaggaggtc ctggcggtgg ccgctcattt ggccagcgcc cgggagctgc gccttcggga
     2461 gctcgccccg gatctcgtcc gcccggtgct cgtcctggga tgctgccctc accgttttcc
     2521 gaaaagccgt ctaaggcgga gccaggcaag cccctttacg cgcgcaagcc gcaacagcgg
     2581 cagcgcccga tggccgacaa gcgcgaaatg gaaggagagc gcaaactcca tccgactcgt
     2641 cagcgccccg gagcaggcgg ccgcggcgtg gctgcggcag cgattgcgcc gcccgaaccg
     2701 cgcgccccgc gcgacattca gttgaccgaa ggcatcacca tccgcgaact cgcggaaaag
     2761 ctcgacgttc gcgccaagga tcttttgaaa acgcttctcg atcgcggcgt tttcgccagc
     2821 attaatcagg cgctagacgt tcaaacagcg acagacctgg cgcaatcctt caacggcgtc
     2881 gtcagcgtcg tgtccctcga agaggaaatg gtactcgagg tcgccaagga agaaactaag
     2941 gaaaatctca aacccaggcc cccggtcgtc accgttatgg gccacgtcga ccacggcaag
     3001 accagccttc tcgacgccat ccgccaggaa gacgtcgcgg ccggcgaggc cgg
//
LOCUS       DATMNS010000100         3718 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2648747, whole genome shotgun sequence.
ACCESSION   DATMNS010000100 DATMNS010000000
VERSION     DATMNS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2648747"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1249
                     /gene="mqnC"
                     /locus_tag="VNW28_03645"
     CDS             161..1249
                     /gene="mqnC"
                     /locus_tag="VNW28_03645"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HXA09049.1"
                     /translation="MVMRNGESLQQKILAGERISAAEALEIYRRPLAELGALADARRN
                     LATGKSYGGRGREIVTYIVDRNINYTNVCNVYCKFCAFYRTEKDADHYVLTREQFDQK
                     LDELTAAGGVQILMQGGHHPTLGFDWYLELLHHIREKYPQINIHGFSPPELQHFAETF
                     KMPLREVIGRFKEAGLGSIPGGGGEILVDSVRQRIAPLKCKSDQWLEVMQVAHELGLK
                     SSATMMFGHVETIEDRVQHLQRLRDQQDASGGFTAFICWTFQPQHTVLKIDQPTGSAE
                     YLRTQALARIFLDNIPNIQSSWVTQGPQIGQIALKYGANDFGSVMMEENVVSSAGTTF
                     RLDAEKIEALVRDAGYEPRRRNNWYELI"
     gene            1415..>3718
                     /gene="bamA"
                     /locus_tag="VNW28_03650"
     CDS             1415..>3718
                     /gene="bamA"
                     /locus_tag="VNW28_03650"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXA09050.1"
                     /translation="MKNLTSSIRLAAVLLSLGLITALAPGARAQGVEGVVINSIDVRY
                     VGPETVSKDRVLANMRTKVGAPYSEATVEDDIRALYDTGKIQNVRIFGEPKGNGVHVQ
                     VILATRALVTEIEIDGAQSFKAKTLRGEIKFKVPSPADAEKLEEGRQNIIDFYQRKGF
                     TGVDVQLQLVTDESRGTARAVYTVKEGEKGAVREIVFQGNTKFSDRTLRKQMKTKAKT
                     PISILDKSGRLDQTQLQQDLDSIREFYQNKGYIDIAIPEVRQERFERGVRIVVVINEG
                     ILYHVGKVTFQGQEATSEARLHTLIKMKPGTIYTPKGLKDDTKAIIDGYGAGGYVDVD
                     LLPQGVPAGPGVVDLSYTITEGSRSFVERVNITGNTRTKDKVIRREILTLPGDVFDTV
                     RVDVSKKRLENLGYFEKVETYPEDTNIPGRKDLLVQVQEKRTGALNFGAGFSTIESLL
                     GFVELTQSNFDLFNYPSFTGGGQKFRLRVQYGTQESSYLLSLTEPYFLDQRFSLGTDI
                     FYREASFLSSVYDQRNYGFAIDARKPLSPFLSVDLNYRLQEIDIFNVALGSSPEILAE
                     EGDNLKSEITPSFVYDTRDSVYLTRHGHRISFTPHIAGGFLGGTVQTYGFDLEASQYF
                     LFPYDIILTLNGETAVIDNWGSGDHVPIFDRLFLGGANDLRGFNFRDVGPKDMNGEPL
                     GGNTLARFTIETTIKVIDRVRAAVFYDTGFINADSYDFGTSHLASDVGVGVRLDLPIG
                     PLKIDYGIPIQKDNNSGGGKVQFSVGYQ"
BASE COUNT          831 a         1138 c         1041 g          708 t
ORIGIN      
        1 ttcccgatct tgcgattaga atctgctgat ggtcgggcac aaccgggaat cgcagcggaa
       61 tgaaatgctc gccgaccaac gcgcttcgac aaaaaaacaa tccactgaca aattcaccgc
      121 gcgaggtgcg gagcgggcgg cactcgtcta gattgcccgg atggttatgc gcaacggcga
      181 atcgctccag cagaagattc tcgccggcga acgcatctcc gccgcggaag cgctggaaat
      241 ctaccgccgg ccgctcgcag agttgggggc gctggccgac gcgcggcgca acctggcaac
      301 gggaaaaagc tacggtggtc gcgggcgcga gatcgtgacc tacatcgtcg atcggaacat
      361 caattacacc aacgtctgta acgtctactg caaattctgc gcattttatc gcaccgagaa
      421 ggacgccgac cactacgtgc tgacgcgtga gcagtttgac caaaaactcg atgaactgac
      481 ggcggccggc ggagtgcaaa tcctgatgca gggcgggcat catcccacac tcggcttcga
      541 ttggtatctc gagctgctcc atcacatccg cgaaaagtat ccgcagatca atatccacgg
      601 attcagcccg ccggagctcc agcatttcgc ggagacgttt aagatgccgc tgcgtgaggt
      661 gatcggccgc ttcaaggaag ccgggctcgg ttccattccc ggcggcggcg gcgagatcct
      721 ggtcgattcc gtccggcaac ggattgcgcc gctcaaatgc aaaagcgacc agtggctcga
      781 agtgatgcag gtggcgcatg agctcgggtt aaaatcgagc gcaaccatga tgtttggtca
      841 cgtcgagaca atcgaggatc gggtgcaaca cctgcagcgt ttacgcgatc aacaggatgc
      901 aagcggtggg ttcactgctt tcatttgctg gacgtttcaa ccgcagcaca ccgtcctgaa
      961 aatcgaccag ccgaccggct ccgccgagta tctgcgcacc caggccctgg cccggatttt
     1021 cctcgataac atccccaaca tccaaagctc ctgggtcacg caggggccgc aaatcgggca
     1081 gattgcgctg aagtacggtg cgaacgactt tggctccgtc atgatggaag aaaacgtcgt
     1141 gagcagcgcc ggcacgactt tccggctcga tgcggaaaag atcgaggcgt tggtccgcga
     1201 cgccggctac gagccgcggc ggcgtaacaa ttggtacgaa ctgatttgat tgctgatggt
     1261 tgattatcgc cagcagattg ccgagtcctc gaccagcaaa tcagccatcg gaaatcagcc
     1321 atctccgaat tcctgccttg acgccgtcgc tcgcattacc catctaatac gcgagtctac
     1381 cgccgacagc cgcctggcga ccgctttctt gtctatgaaa aatctcacct cgtccatccg
     1441 cctcgccgcg gttctccttt cgttaggctt gatcactgcg ctcgcgccgg gcgcgcgcgc
     1501 ccaaggggtg gagggcgtcg tgatcaattc gatcgatgtg cgctacgttg gtcccgaaac
     1561 cgtttccaag gatcgcgtcc tcgccaatat gcggacgaaa gtcggcgcgc cttattccga
     1621 ggcgacggtg gaagacgata tccgcgcgct ctacgacact gggaagatcc agaatgtgcg
     1681 catctttggc gaaccgaaag gcaacggcgt ccatgtgcag gtcatcctcg ccacccgcgc
     1741 gctggtgacc gagatcgaga tcgacggcgc gcagagtttc aaggcgaaga cgctgcgcgg
     1801 cgagatcaag ttcaaagtgc cctcgcccgc cgacgcggag aaactcgagg agggccggca
     1861 gaacatcatc gacttttatc agcgcaaggg cttcaccggc gtcgacgtcc aattgcagct
     1921 ggtaacggac gaaagccggg gcacggcgcg cgcggtttac accgtcaagg agggcgagaa
     1981 aggcgcggtg cgcgagatcg tattccaggg caacaccaaa ttcagcgatc gtacgttgcg
     2041 gaagcagatg aagaccaaag ccaagacccc aatctcgatc ctggacaaat cgggacggct
     2101 ggatcagacg cagttgcaac aggatctcga cagcatccgc gagttttacc agaacaaggg
     2161 ctacatcgac atcgccatcc ccgaagtgcg ccaggaacgc tttgaacgcg gggtgcgcat
     2221 cgtggtggtg atcaacgaag gcattctata ccatgtgggg aaagtgacgt tccaaggcca
     2281 ggaggcgacc tcggaagcaa ggttgcacac cctaatcaaa atgaagccgg gcacgatcta
     2341 cacgcccaag ggattgaagg atgacaccaa ggcgatcatc gacggctacg gcgcgggcgg
     2401 ctacgtggat gtggaccttc tgccgcaagg ggtgccggcc ggcccaggcg tggtcgacct
     2461 gagctacacc attaccgaag gctcccgttc ctttgtcgag cgggtcaaca tcacaggcaa
     2521 cacgcgcact aaagacaaag tgattcgccg cgaaatcttg accttgcccg gcgacgtctt
     2581 cgacaccgtg cgcgtcgacg tcagcaaaaa gcgactcgaa aacctgggct atttcgagaa
     2641 agtcgaaacc taccccgagg ataccaacat cccaggccgg aaggatctcc tcgtccaagt
     2701 ccaggagaaa cgcaccggcg cactcaattt cggcgccggt ttcagcacga tcgagagctt
     2761 gctcggcttc gtcgagttga cccagagcaa cttcgacctc ttcaactatc ccagcttcac
     2821 cggcggcggc caaaagtttc gcctccgcgt ccagtacggg acccaggaat ctagctacct
     2881 cctctccctg acggagccct attttctcga tcaacgcttt tcgttaggga cggatatctt
     2941 ttaccgcgaa gccagtttcc tcagctcggt ctacgatcag cgcaactacg gcttcgcgat
     3001 cgatgcccgg aagccgctct cgcccttcct ttccgtcgat ctcaattatc ggctgcagga
     3061 aatcgacatt ttcaacgtgg ccctgggctc gtcgccggaa atcctggccg aagagggcga
     3121 caatctgaaa agcgaaatca cgccctcttt cgtctatgat acgcgcgaca gcgtttatct
     3181 cacccgccac ggtcatcgca tttcctttac gccccacatt gccggcggct ttctcggcgg
     3241 aactgttcaa acctacggct tcgatctgga agcctcgcaa tatttccttt ttccttacga
     3301 catcattctc acgctcaatg gagaaaccgc ggtcatcgac aactggggga gcggggatca
     3361 cgtccctatt ttcgaccgct tgttcctggg tggcgcgaat gacctccggg gcttcaactt
     3421 ccgcgacgtc gggccgaaag acatgaatgg cgaaccgctc ggcggcaaca cactcgcccg
     3481 cttcacgatt gaaaccacga tcaaggtcat cgaccgcgtg cgcgccgccg ttttctacga
     3541 cacgggcttt atcaacgccg attcctacga cttcggcacc tcgcacctcg cttccgacgt
     3601 cggcgtcggc gtgcgcctcg atctcccaat cgggccgttg aagatcgact acggcatccc
     3661 catccaaaag gacaacaact cgggcggcgg caaagtccag ttcagcgtcg gctaccaa
//
LOCUS       DATMNS010000101         6825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4799151, whole genome shotgun sequence.
ACCESSION   DATMNS010000101 DATMNS010000000
VERSION     DATMNS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4799151"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VNW28_03655"
     CDS             <1..699
                     /locus_tag="VNW28_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HXA09051.1"
                     /translation="RRDRLQKEIRAGSKTAKAEAALIEKLLPHLDSGKPAITLTLTDE
                     EKITVREFFLLTSKPTLFACNVGESDLAAIVEKDEANAARRHVRAVEEYARNHFATEA
                     VVISAQIESELVDLSEAEAAEYLDDLGVKESGVGALIRAVYHLLGLRTYLTTGEKETR
                     AWTINEGDKAPAAAGVIHSDFERGFIAAETVHFDDLVSLGSFAKAREAGKLRIEGKEY
                     VVKDGDVVEFRFNV"
     gene            complement(806..1510)
                     /locus_tag="VNW28_03660"
     CDS             complement(806..1510)
                     /locus_tag="VNW28_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HXA09052.1"
                     /translation="MQVELEQLLILQDRAQKIRQVDAELKSLPLERKSLDAQVAVASA
                     NLVATKDRARHVEMEKKKLELDVGTREESIARLKTQQYETRKNDEFSAMGREIERYQN
                     EISALEDQELELMEQADVLKAQIATEEKQTATAKESIAKQIADLGAKEATLGIRLEEL
                     KKEREELAAKVDEDLLDRFNRLFASKGDAAVVALEHDVCTGCHMKITTQTAVRVKGGQ
                     EIVSCEQCGRILYFAE"
     gene            complement(1635..2882)
                     /locus_tag="VNW28_03665"
     CDS             complement(1635..2882)
                     /locus_tag="VNW28_03665"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXA09053.1"
                     /translation="MRRAVITGLGPITSVGIGRDGFWNGLRAEKSGIRRVESFDSSIF
                     NAHCAAEIPDWAPEEFFPPHRLKRLDRYAQFAVASAKLALDDAGIEWSRENPQARVGV
                     SFGTALGGIANAESEHAHFLKKGSRGVNQTLALQVFGGSAHTNIAIEFGFRGAGTTNS
                     NSCASGTIAVGEALRYIRDDFADVIVAGGAESPLSQLTFGAFAFIKTMSQFAGDPAQA
                     CRPFDRLRDGFVMGEGAASLIIEELEHARARGAHIYGEVLGYSLNNDAFHMTSPLPSG
                     EACIRAMRETLADAGLVPEQIDYINAHASSTQLNDSTETRAIKEVFGPHAARIPVSGT
                     KPYTGHPLGATGAIEAALCALTIDRGWVPPTLNWGNPDPACDLDVVPQYGREVAVDHV
                     LSNSFGFGGINACIVLGRVPALD"
     gene            complement(2986..3462)
                     /locus_tag="VNW28_03670"
     CDS             complement(2986..3462)
                     /locus_tag="VNW28_03670"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXA09054.1"
                     /translation="MQTGNSIIMHAPREAIFETAADLAQWPRILPHYRFIRFLDRSPD
                     RSIVEMAALRSGIPISWTSEFVVDRERMELRFLHLKAFTRGMRVIWTFKETPAGVLVE
                     ITHTLRFRVPALAPVAEPIIGGFFIHHVANQTLYAMKAHLEEKSEIRMSKSETISK"
     gene            3763..5520
                     /locus_tag="VNW28_03675"
     CDS             3763..5520
                     /locus_tag="VNW28_03675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09055.1"
                     /translation="MLSSSPLLLARLRQFLGSTEEEATARQMRPWFAVAMLYAVGCAL
                     LALHQAFASHYMVADDVREHVFWMFRYLDSRLFAHDPIADYFQSLAPSGYSSLYWLLA
                     HAGIDPLLASKLIPSVLSLFATGYFFLLAWRVLRSPAAATLTAILFCQCLWSNSDLCS
                     ATPRAFFYPLFAAFLYYQVRGSLIGVLVTVGLEVLFFPPAALLSLGVLGWSCLCWKNG
                     PRLVPIKGSQVYLVFSAGFVLTLLLTWPYLHSVGTFGPLVTYTEARRMPEFGPHGRVP
                     FFLPSWWGYWVGGNGGIHTLPTRPPWFLAAFLWPVLRRFPNRFPLLKAVPKGVRPVPQ
                     MIGAALSLFLVAHLLLFRLYLPNRYTQSSTRVILILLAGGVLLALIDAALLWAEKRPV
                     KSQRVAKAVALGFCALLLGGIIAFPLLLPTFPTGSYVKGTDPGLYQFFARQPLTIRIA
                     SLSDEANNLPIFSRRSIIVGAECAVPFHPGYYLPLRARGLQIARAQYSAGLTVLQKCL
                     RDQQVDFWLLDRAAFSPDYRRKSRLLRQLDLVTPDQPIGPASGAIPFLERPPPETVAY
                     EDARFVVLDTRRLLALQPR"
     gene            complement(5517..6326)
                     /locus_tag="VNW28_03680"
     CDS             complement(5517..6326)
                     /locus_tag="VNW28_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RibD family protein"
                     /protein_id="HXA09056.1"
                     /translation="MRSRKTSKSPEGRASARPVSVPKARAILDVQKHVAPKRRLRPFV
                     AATFAMTVDGKITTRAFSPVDFTSREDKLHLLRQRSLGDAVLIGRSTLAHDNVRLGLP
                     DPKMRAAREARGQSPYPLRVIVSNEGRIDPALKIFQTEISRIVIFSTTRMPRKFQVGL
                     RDKATLHLTEARCVDLARMLQQLRREYGVRRVACEGGAELFRSLLELRLVDQLNLTIA
                     PYIFGGKGAPTLTGRGRDFLPASIFCSLVEMRVIGKECFLVYRLRYPEASP"
     gene            complement(6304..>6825)
                     /locus_tag="VNW28_03685"
     CDS             complement(6304..>6825)
                     /locus_tag="VNW28_03685"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HXA09057.1"
                     /translation="LVTSFVVSGFHLAHQLPHRFPAREKLIELSAAYHLYARHWRATL
                     VAFGASIIAHLATFGTFLCVAYAFRAQVAWVDFFAIMPVERTISSLPISFAGVGLREK
                     IFQIMLHGLCGVPEAVAVLIGSMSFLVMLACSAPGGIVYFLYKPSGQTGHVKLSEMRK
                     EVATLEHEITENE"
BASE COUNT         1261 a         2131 c         2014 g         1419 t
ORIGIN      
        1 cgccgcgacc gactgcaaaa agaaatccgc gccggatcga agacggcgaa agccgaggcg
       61 gcgttaatcg aaaaactcct gccccatctg gattccggga aaccagcgat cacgctaacg
      121 ctgaccgacg aagaaaaaat cacggtgcgc gaatttttcc tgctcacctc caagccaacg
      181 ctcttcgcct gcaacgttgg cgaatccgat ctcgccgcga tcgtagagaa agacgaggcg
      241 aacgccgcgc ggcggcatgt gcgagcggtc gaagaatacg cgcggaatca tttcgcgacc
      301 gaagccgtcg tcattagcgc gcagattgag agcgagttgg tcgatctctc cgaagccgaa
      361 gcagccgaat acctggatga tctcggcgtc aaggaaagcg gcgtcggcgc actcatccgc
      421 gccgtctatc acctgctcgg attacgcacc tacctgacga ccggcgaaaa ggaaacgcgc
      481 gcctggacga tcaacgaagg cgacaaggcg ccggcggcgg cgggggttat ccactccgat
      541 ttcgagcgcg gcttcatcgc ggccgagacg gtgcacttcg acgacctggt gtcgttaggc
      601 tccttcgcca aggcacgcga agcgggcaag ctgcggatcg aaggcaagga atacgtcgtc
      661 aaagacggcg acgtggtgga gttccgcttc aacgtctgac gacgcccgga gggacgtgct
      721 tccgcacgtc cggtctggta gcgacgcgcg ctcgaacaac caggacgtgc ggaagcacgt
      781 ccctccagtt ggttcgagcg ggccgctatt ccgcgaagta gagaatccgg ccgcattgtt
      841 cgcagctcac gatctcttga ccacctttca cgcgcacggc ggtctgggtg gtaatcttca
      901 tgtggcagcc ggtgcaaaca tcgtgctcca gcgccaccac cgccgcgtcg cctttgctcg
      961 cgaaaagccg gttgaagcgg tcgagcaaat cttcgtccac tttcgcggcg agttcttcac
     1021 gctccttctt caattcttcg aggcggatgc ctaacgtcgc ctctttcgcc cccaggtcgg
     1081 cgatttgttt ggcgatcgat tccttcgccg tcgccgtttg tttttcctcg gtggcgatct
     1141 gcgccttgag cacatcggcc tgttccatca gctcgagctc ctgatcctcg agcgcgctaa
     1201 tttcattttg ataacgctca atctcgcgcc ccatcgcgga gaattcgtca ttcttgcgcg
     1261 tctcgtattg ctgtgtcttc aagcgcgcga tgctctcctc gcgggtgccg acatcgagtt
     1321 ccagtttctt cttttccatc tcgacgtgac gcgcccgatc cttggtcgcg acgaggttcg
     1381 ccgaagctac tgcgacttgc gcatcgagcg atttccgctc gagcggcaaa ctctttagtt
     1441 ccgcgtcgac ttgccgaatt ttctgggcac gatcctgcaa aatgaggagc tgctccaact
     1501 cgacttgcac gcgcgcatgt taggagcgcg caggggcggc gtcaacgacg cggcgatgcg
     1561 ccgcaggaga ttgtaggaca accgctccgg ttgcccgacg acaggcggag cgcccgccct
     1621 accaaatgcg atcgttagtc gagcgctggc acccgcccga gaacaatgca ggcattgatc
     1681 ccgccgaagc cgaacgagtt gctcaggaca tgatcgaccg ccacctcccg tccgtattgc
     1741 ggaacgacat ccagatcgca ggccgggtcg ggatttcccc aattcaaggt cggcggcacc
     1801 cagccgcgat caatcgttag ggcacaaagc gccgcctcga tcgcgccagt cgccccgagc
     1861 ggatgaccgg tatagggctt tgtcccgctg accgggatcc tggcggcatg cgggccgaag
     1921 acttccttga tcgccctggt ctcggtgctg tcgttaagct gggtcgaact ggcgtgagcg
     1981 ttgatgtaat cgatctgttc tggcacaagg cccgcgtcgg ccagcgtctc gcgcatcgcg
     2041 cgaatgcacg cctcgccgct cggcagcggc gaagtcatat gaaaggcgtc gttgttcaaa
     2101 ctgtaaccga gcacctcccc gtagatgtgc gcgccgcgcg cgcgggcgtg ctccagttcc
     2161 tcgatgatta acgacgccgc cccctcgccc atcacgaaac cgtcgcgcaa cctatcgaac
     2221 ggccggcagg cctgcgcggg atcaccggcg aactggctca tcgtcttgat gaacgcgaag
     2281 gcgccgaagg tgagctgact gaggggcgat tccgcgccgc cggccacgat gacatcggca
     2341 aaatcgtcgc gaatatagcg gagcgcttcg ccgaccgcga tcgtgccgct ggcgcaactg
     2401 ttcgagttcg tcgtccccgc gccgcggaaa ccaaactcga tcgcgatgtt ggtgtgggcc
     2461 gagccgccga agacctgaag cgcgagcgtc tggttcactc cacggctgcc ttttttcaaa
     2521 aaatgcgcgt gctcggattc ggcgttcgcg attccgccga gcgcggtgcc gaaactcact
     2581 ccgacgcgag cctgcgggtt ttcgcgcgac cattcgatcc ccgcgtcgtc gagcgcgagc
     2641 ttggccgagg cgaccgcaaa ctgcgcgtaa cgatcgagcc gcttcaaacg atgcggcggg
     2701 aaaaattctt ccggcgccca atcagggatt tcggccgcgc aatgcgcgtt gaagatcgag
     2761 ctgtcgaaac tttccacccg gcgaatgccg cttttctccg cgcgcagccc gttccaaaaa
     2821 ccatcccgcc cgatgccgac ggaggtgatg ggaccgaggc cggtgatgac cgcgcgcctc
     2881 atgggggaaa attcgaatgt cgaatatcga atatcgaaac aaatccgaaa tccgaaatcc
     2941 gaaacgaagc tcccgcgctc ggctgttttg aattttgaat tttcgtcatt tggaaattgt
     3001 ttcggacttc gacattcgga tttcggattt ctcctcgagg tgcgctttca tcgcgtagag
     3061 ggtttggttc gccacgtgat ggatgaaaaa gccgccgatg atcggctccg ccaccggcgc
     3121 gagcgcgggg acccggaagc gcaacgtgtg cgtgatctcg accagcaccc cggcgggcgt
     3181 ttccttaaac gtccagatca cgcgcatacc ccgggtgaac gccttcaaat gcagaaagcg
     3241 gagttccatc cgttcgcgat cgaccacgaa ctccgacgtc caggaaatcg gaatgcccga
     3301 gcgcagcgcc gccatctcca cgatgctccg atccgggctg cggtcgagaa aacggatgaa
     3361 gcgataatgc ggcaagatgc gcggccactg tgcgagatcc gcggcggtct cgaagatggc
     3421 ttcgcgcggc gcgtgcatga tgatggagtt gccggtctgc atggagcgcg taaaaagagg
     3481 agcgtcagga taagcggcga acgtcgtcgc gcaatgaatg gacgagagta tgaccctgga
     3541 aattcgtaac aaatgtgaaa gatgcgaaaa gccggttgcg ccggcggatg aagcctacat
     3601 ctgcaccttc gaatgcactt tctgcgcgga atgcgccggg gccctggaaa acatttgtcc
     3661 caactgcggc ggcgagttgg tccggcgccc ccgtccgccg gacaccactt gactgccatt
     3721 cgttctctct catccaaggt ggttgatttc gcgcgtcctt ccatgctgtc ttcttcgccg
     3781 ttgctcctcg cccggttgcg ccaatttctg ggttccacgg aggaggaggc gacggcgcgc
     3841 caaatgcggc cgtggtttgc ggtcgccatg ctctacgcgg tgggctgcgc ccttctcgcg
     3901 ttacatcagg cgttcgcatc ccattacatg gtcgccgatg acgtccgcga acatgtcttc
     3961 tggatgttcc gctaccttga ctcccggctt ttcgctcatg atcccatcgc ggattacttt
     4021 caatctctcg cgccgagcgg ctattcctcc ctttattggt tattagcgca cgcggggatc
     4081 gatcccttgc tcgcgagtaa gttgattccg tcggtcctca gcctgttcgc gaccggatat
     4141 ttcttcctgc tcgcgtggcg cgttctccgc tcgccggccg cggccaccct gaccgcgatt
     4201 cttttttgcc agtgcctttg gtcgaatagc gatctttgtt ccgccacgcc gcgcgcgttc
     4261 ttctatccgc tctttgccgc cttcctttac tatcaggtgc gcggttcgct catcggagtc
     4321 ctggttacgg tcggtctcga ggttcttttt ttcccgccgg ccgctttact gagtctcggc
     4381 gtgctgggtt ggagttgtct gtgttggaaa aacggtccgc ggcttgttcc catcaaaggc
     4441 tcgcaagtgt atctcgtttt ttccgcgggc ttcgttctca ccctcctctt gacgtggccc
     4501 tatctccata gtgtcggcac ttttggccca ttggtgactt ataccgaggc acgacggatg
     4561 ccggagtttg gccctcacgg ccgggtgcct tttttccttc cgtcctggtg gggttactgg
     4621 gtgggcggaa acggagggat acacactctc cctacgcgtc cgccgtggtt tctggccgcc
     4681 tttctctggc cggtgctgcg ccgtttcccg aatcggtttc ctttactcaa ggccgtgccc
     4741 aaaggcgtgc ggcctgtgcc gcagatgata ggcgcagctc tttcgctctt cctcgtcgcg
     4801 catcttttgc tttttcgcct ctaccttccc aatcgctaca cgcaatcgag cacgcgcgtt
     4861 atcctcatcc tgctcgccgg cggagtgtta ctcgcactga tcgatgccgc cttgctctgg
     4921 gcggaaaagc gaccggtcaa gagtcagcgg gtcgcaaaag cggtcgcgct cgggttctgc
     4981 gctctcctgc tcggcggcat tatcgctttt cccctgctcc ttcccacgtt cccgaccggc
     5041 agttacgtca aaggcactga cccaggcctt taccagttct ttgcccgtca accgctgacg
     5101 attcgtatcg cctccctctc cgacgaagca aacaacctcc ccatcttctc tcgccgttcc
     5161 attatcgtcg gggcggaatg cgcggtgcct tttcatcccg gttattacct gcctctgcgc
     5221 gcgcgcgggc tgcaaatcgc gcgcgcccaa tacagcgctg gcctcaccgt tctgcaaaag
     5281 tgtttgcgcg accagcaggt cgatttctgg ctcctggatc gtgcggcgtt ttcgcccgat
     5341 taccggcgaa aaagtcggct cctgcgacag ctcgacctgg tcactccgga ccaacccatc
     5401 ggtcccgcgt caggagcgat cccttttctc gagcgaccgc cgccagaaac ggtcgcctat
     5461 gaagatgcgc gcttcgtcgt gctcgatacg cgccgtctgc tcgcgctgca accccgctag
     5521 gggctggctt cgggatacct gagccggtag accaggaagc attccttgcc gatgacccgc
     5581 atctcgacta acgaacaaaa gatgctggcc ggcaggaaat ccctgccacg tccggtgaga
     5641 gttggcgcgc ctttcccgcc gaagatgtag ggcgcgatcg tcaggttaag ctgatcgacc
     5701 agccgcaact cgagcaggct gcgaaataac tcggcgccac cttcgcaggc gacccggcgc
     5761 acgccgtatt cgcgacgcag ttgttgcaac atgcgggcga gatcgacgca acgcgcctcg
     5821 gtcaggtgga gcgtcgcttt atcgcgcaag cccacctgga atttccgcgg catgcgggtg
     5881 gtcgagaaga tcacaatgcg cgagatctcg gtttggaaaa ttttcagtgc gggatcgatc
     5941 cgaccttcgt tggaaacgat cacgcgcagc gggtaaggcg attgcccgcg cgcttcgcgc
     6001 gccgcgcgca tcttcgggtc gggcaggccg aggcggacgt tgtcgtgcgc gagggtgctg
     6061 cgcccaatga ggacagcgtc gcctaacgaa cgttggcgca gaagatggag cttgtcttcg
     6121 cgggaggtga aatcgaccgg ggaaaaggcg cgggtggtga ttttaccatc cacggtcatg
     6181 gcgaaagttg ccgcgacgaa cggccggagc cgtcgtttcg gagcgacgtg cttctgcacg
     6241 tccaagatgg ctcgggcctt agggacagag actggccgtg cagaagcacg tccctccggg
     6301 ctcttactcg ttttccgtga tctcatgctc cagggtcgcc acttccttgc gcatctcgct
     6361 tagcttcacg tgcccggtct ggccgctggg tttgtagagg aaataaacga tcccgcccgg
     6421 cgcggagcag gccagcataa cgagaaaact catcgaaccg atcagcaccg cgaccgcttc
     6481 cggcacgccg cagaggccgt ggagcatgat ttggaaaatc ttctcccgca gtccaacgcc
     6541 cgcgaagctg atcggcaacg aagagatcgt gcgctcgacc ggcatgatag cgaagaaatc
     6601 gacccatgcg acctgcgccc gaaaagcgta ggccacgcaa agaaaagttc caaaggtggc
     6661 caaatgcgcg atgatcgacg cgccgaaggc aaccagcgtc gcgcgccagt gacgcgcgta
     6721 gagatgatag gcggctgaga gttcgatcag tttttcgcgg gcgggaaagc ggtgtggtaa
     6781 ctggtgggcc aggtgaaatc cgctgaccac aaacgaagtc accag
//
LOCUS       DATMNS010000102         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3655287, whole genome shotgun sequence.
ACCESSION   DATMNS010000102 DATMNS010000000
VERSION     DATMNS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3655287"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..649
                     /locus_tag="VNW28_03690"
     CDS             <1..649
                     /locus_tag="VNW28_03690"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXA09058.1"
                     /translation="YRAAEILETRRLELEAELARATGRKDGKATDEASLTIERLVHYA
                     GWTDKFSQIFSAVNPVASSHFNFTLPEPTGVVVVVCPNEPPLLALVSLVAPVILSGNT
                     AVVLTSTTQPLPALTFSEIIATSDLPGGVINLLSGDPAELAPHIAGHMDVNAIVDASG
                     DPKIGLALQQGGGFNVKRYVPRTMKSADWLGEMAANPYWILDTVEMKTAWHPIGL"
     gene            713..1945
                     /locus_tag="VNW28_03695"
     CDS             713..1945
                     /locus_tag="VNW28_03695"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HXA09059.1"
                     /translation="MHIPTLIERKRDGNELASDEIGALVDGFTRGAIPDYQMSAFAMA
                     VFFRGMTPNETQHLTAAMMKSGRILQYPGDSPPKVDKHSTGGIGDKVSLVLAPLLACD
                     EVWVPMISGRGLGITGGTLDKLESIPGFNVNLDEPRALRQLEKIRVCMIGQTADICPA
                     DKKLYALRDVTGTVSSQPLIVASIMSKKLAENLDRLVLDVKFGSGAFMKTRAEAAQLA
                     EAMKNVGARMGVKISHLLSPMNEPLGRAVGNALEVAEAVDTLQGRGPNDLVQLTLDLA
                     SEVATAPRAQLARWLIDGSAWRKFIELVEAQDGDASALEQIATIHAAPVIHPFHTPRG
                     GEIKRMDAETIGRAALFLGAGRARSDDAIDFAVGFSRIKKVGERVVPNEPLLFVHART
                     DQALASVLPLLEKAIEVA"
     gene            1954..>2239
                     /locus_tag="VNW28_03700"
     CDS             1954..>2239
                     /locus_tag="VNW28_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09060.1"
                     /translation="MMVKSLPLLVAASTAFLALGGVLAWVVARLLRSNRAQLVSSGPM
                     TAAQEFTLREPGTLLLLVEVPRFGSDFRQLEFEVVEQATGQATKLRYDFVR"
BASE COUNT          440 a          678 c          712 g          409 t
ORIGIN      
        1 gtaccgcgcc gccgagatcc tggaaacgcg ccggctggaa ttggaagccg aactcgcccg
       61 cgcgaccggc cggaaagacg gcaaagccac cgacgaagcc agcctgacga tcgaacgtct
      121 cgtgcactac gcgggctgga cggacaaatt cagccagatt ttcagcgcgg tgaatccggt
      181 cgccagctcg cacttcaact tcaccctgcc ggaaccaacc ggcgtggtgg tggtggtttg
      241 ccctaacgaa ccgccgctgc tcgcgctggt ttcgctggtc gcgccggtca ttctgagcgg
      301 caacaccgcg gtcgttctca cctcgaccac gcagcccctc cccgccctga cgttttcgga
      361 aattatcgcg acgagcgatc tgccgggtgg agtgattaat cttctttccg gcgatccggc
      421 tgagctcgcg ccgcacattg ccggccacat ggacgtgaat gcgatcgtgg acgcgtccgg
      481 cgatccgaag atcggactgg cgctgcaaca gggcggcggg ttcaacgtga aacgctacgt
      541 gccgcgcacg atgaagtcag cggactggct cggcgagatg gcggcgaatc cgtattggat
      601 tctcgacacg gtcgagatga agacggcctg gcacccgatc ggactttaag agaaggaaga
      661 attaagaaag cagaaggaag aaggccggcg ggagattctt tctttttggc ccatgcacat
      721 ccccacgctg atcgagcgca aacgcgacgg aaacgagctt gcatcggacg agatcggcgc
      781 gctcgtcgat ggcttcaccc gcggcgcgat cccggattat cagatgagcg ccttcgcgat
      841 ggcggttttc tttcgcggaa tgacgcctaa cgagacccag catctgaccg ccgcgatgat
      901 gaagagcggc cggatcctgc agtaccctgg cgactcgccg ccgaaagtgg acaagcattc
      961 cacagggggg atcggtgaca aagtctcgct cgtgctcgca ccgttgctgg cctgcgacga
     1021 ggtctgggtg ccgatgattt ccggacgcgg gctcggcatc accggcggca cgctcgacaa
     1081 actcgaaagc attcccgggt tcaacgtgaa cctcgacgag ccgcgcgcgc tgcggcaatt
     1141 ggagaagatt cgggtctgca tgatcggcca gaccgccgac atttgtccgg cggacaaaaa
     1201 actctatgcg ctccgcgacg tgaccggcac ggtgtcgtcg caaccgttga tcgtcgccag
     1261 tatcatgagc aagaagctgg cggaaaatct tgatcggctc gtgctcgacg tgaagtttgg
     1321 gagcggcgct ttcatgaaga cgcgcgcgga agccgcgcaa cttgcggaag cgatgaaaaa
     1381 cgtgggcgca cgaatgggcg tgaagatttc gcatctgctc agcccgatga atgaaccgtt
     1441 aggccgcgcg gtggggaatg cgctggaagt agcggaagcc gtcgacacct tgcagggccg
     1501 tgggcctaac gatctcgtcc agcttaccct cgatctcgcc tcggaagtcg cgaccgcgcc
     1561 gcgcgcgcaa ctggcacgct ggttgatcga cgggagcgcg tggcggaaat tcattgagct
     1621 ggtcgaagca caggacggcg acgcctccgc cttggaacaa attgccacta tccacgccgc
     1681 gcccgtcatc catcccttcc acacgccacg cggcggagag atcaaacgga tggacgcgga
     1741 gacaattggg cgggccgcgc ttttcctcgg cgccggtcgg gcccgttccg atgacgcgat
     1801 cgatttcgcc gtcggctttt cgcggatcaa gaaagtcggc gaaagagtcg tgcctaacga
     1861 accgctgctt ttcgttcacg cgcgtactga tcaggcgctg gcctcggtgc tgccgctgct
     1921 cgaaaaggcc atcgaagtgg cctaactatt ccgatgatgg tcaaatcatt gcctctcctg
     1981 gtcgcggcga gcaccgcttt cctcgccctg ggtggtgtgc tggcctgggt cgtggcgcgg
     2041 ttgttgcgca gcaaccgcgc acaattggtc tccagcgggc cgatgacagc ggcgcaggaa
     2101 ttcacgctgc gcgaaccggg gacacttctg ctcctggtcg aagtgccccg cttcggatcg
     2161 gactttcgcc agcttgaatt cgaggtggtc gagcaggcca ccgggcaagc aacgaagttg
     2221 cgttacgatt tcgtgcgcg
//
LOCUS       DATMNS010000103         6350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_152214, whole genome shotgun sequence.
ACCESSION   DATMNS010000103 DATMNS010000000
VERSION     DATMNS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152214"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /gene="dnaG"
                     /locus_tag="VNW28_03705"
     CDS             complement(<1..1300)
                     /gene="dnaG"
                     /locus_tag="VNW28_03705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HXA09061.1"
                     /translation="MGTIPNHTIEQVAAANDIVEVIGSYFPLKRAGANFRALCPFHQE
                     KTPSFHVNPARQSFHCFGCGVGGSVFRFVMDYEHIDFPASVRRLAARAGIPVIEERGG
                     SAEDDRQHEARRTLLRLHAEAAEWFHENLLKREFAAPARDYLKQRGISSAVVKSWQLG
                     FAPESWDACLKWALDQGYKRGTILQSGLVKLRDEERPESEVYDRFRGRLMFPICNDVG
                     EVIAFSGRILTSDKEAAKYLNSPETPLFRKGSILFGLHKTKRGLIDADCAIVCEGQLD
                     LITLFEAGLTNVVAPQGTAFTDGQARILKRYVNEVVLCFDADAAGQKAAERSLEPLLE
                     NNLVVRVAEMPPGEDPDSLVRKEGADKFGARIAAARDFFDFWIEREAASASLTSLSEK
                     MKLARRVAEVVSRVRDQNTRHEISVRAASRLAVPTRDFLAL"
     gene            1436..2830
                     /locus_tag="VNW28_03710"
     CDS             1436..2830
                     /locus_tag="VNW28_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09062.1"
                     /translation="MLRILIIFLVATLSLGAAAAGEVIAPGSVVVAPLHGEVSPSLAL
                     FLRRAQKAAENDGATAIVLDMNTYGGELDSAEQITDILNHATIPTYTYINTNAGSAGA
                     IIALSTRHIYMAPVSAIGAAAPILSTGEDLPPTQKEKTVSYWSALVRGAALKNGHNPD
                     IAEAFINKEKEVRIGDRVIHPKGSLLTLNAQDATEKINGKPVLAEGVADSLHELMQKA
                     GLKGEVVEFDPSGFEQLAFWLTALSPLLLLGGILGAYLEFKIPGFGLAGIVSVVCFAL
                     FFFGHYFAGLAGWEVVGLFVLGVFFVLVEVLFFGHSTIVFGVIGVLLMFASLLWAMID
                     RYPGQKLVPTGQMLRIPLLNMLLTFAAAAVLIVLLARFLPQTSIYRRFILSTTNPPGP
                     SLAGAPRAFATALPLTPGMRGRSLTILRPSGKAEFESNVVDVVTEGEFIAPETPVAIV
                     STDGMRVVVRRIAA"
     gene            complement(2962..3035)
                     /locus_tag="VNW28_03715"
     tRNA            complement(2962..3035)
                     /locus_tag="VNW28_03715"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2999..3001),aa:Arg,seq:ccg)
     gene            3159..3497
                     /gene="rplS"
                     /locus_tag="VNW28_03720"
     CDS             3159..3497
                     /gene="rplS"
                     /locus_tag="VNW28_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXA09063.1"
                     /translation="MNIIDTIAKEQQKTDVTKFNVGDGVRVHTRVREGDKERIQIFSG
                     IVIGRKGTGLNQTFTVRRISYGEGVERVFPVHSPRIAKVEVEKQGRARRAKLNYLRNR
                     KGKEATAVRE"
     gene            3606..4229
                     /locus_tag="VNW28_03725"
     CDS             3606..4229
                     /locus_tag="VNW28_03725"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXA09064.1"
                     /translation="MYRRCGFRHEKKLRSLGIGCVAGIDEAGRGALAGPVVAAAAILP
                     ERFRHRKLNDSKQLSPELREEIYEYLTNHSEIVWAVGIVDSVEIDRINILRASHQAMR
                     IAVVGLTVSPEHVLIDGLPVFPFPLPQTAIVDGDCLSLSIAAASVIAKVTRDTLMREF
                     CARFPQYCFSQHKGYGTELHLQKLHEHGPCPIHRRSFEPVAQPVFSF"
     gene            4252..4611
                     /locus_tag="VNW28_03730"
     CDS             4252..4611
                     /locus_tag="VNW28_03730"
                     /inference="COORDINATES: protein motif:HMM:NF009150.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXA09065.1"
                     /translation="MRGEKLAARHLRGHGFKILYRNFRGRQGGEIDLVCRERDTLVFV
                     EVKTRTREDFGRPLEAVNRRKQRRISLGGLAWLRLLGNPDILFRFDVVEVTMADETAP
                     RVELIRNAFQLSDPYIY"
     gene            4744..6057
                     /locus_tag="VNW28_03735"
     CDS             4744..6057
                     /locus_tag="VNW28_03735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor tandem repeat GloVer-containing
                     protein"
                     /protein_id="HXA09066.1"
                     /translation="MTTANRMGRGGKTATGLAGKCASAMAVVRSGREMVHLAAGAFVL
                     SAVLFSGAASKGATTEVIYSFAGEDDGEYTDTDVAIDAAGNLYGTSVLGGEFGGGTVW
                     RLSPVGDSWVHTVLYSFTGGADGGEPYKGVTLDAAGNLYGTAVTGGSGSCEGGCGVAY
                     KLTKSSDTWTQSVIHAFTGEDGSGPGARVVVDDRGNVYGTTPTGGANGLGVIYALHSN
                     ANGDWMLRVIHTFTGGSDGSSGSAGQLVPRGGHVYGAATTGGVDGAGVIFELTPTQIG
                     EWGFRTIYSFQGAPDGSFPYGALLFDPAGNLYGTTYYGGTNGLGAVYKLSLTGTREWS
                     ETVLYSFQTGSDGNSSIGNLVADAAGNLYGTTSEGGLGSGTIFELTPGQGGTWTESLP
                     HSFQGPPDGAFPYAGMVGDGAGNFYGATVHGGDADGDGAIYKFTP"
BASE COUNT         1213 a         1859 c         1933 g         1345 t
ORIGIN      
        1 tcaaagccaa aaaatctctc gtgggtacgg ctagacgaga agcagctcta accgaaatct
       61 cgtggcgggt attttgatca cgcacgcgtg aaacaacttc agcaactcga cgagcaagct
      121 tcattttttc gctgagtgac gtcaggctgg cgctagctgc ctcgcgctcg atccaaaaat
      181 cgaaaaaatc gcgcgcggcg gcgatccggg cgccgaattt gtccgcgcct tccttccgga
      241 ccaacgaatc gggatcttct cccggcggca tttcggccac gcggacgacg agattgtttt
      301 ccaacaacgg ctccagggag cgctcggccg ccttttgtcc ggccgcatcg gcgtcgaaac
      361 agagcacgac ttcgttgacg tagcgtttca aaatgcgcgc ctggccatcg gtgaaggccg
      421 tgccctgggg tgcgacaaca ttcgtcagtc cggcttcgaa aagcgtgatc aggtcgagct
      481 gaccctcgca aacgatggcg cagtctgcgt cgatcaagcc gcgcttggtt ttgtgcaggc
      541 cgaacaggat gctgcccttg cggaagagcg gcgtctcggg cgagttgaga tatttcgccg
      601 cctccttgtc gctcgtgagg atgcgcccgc tgaaagcaat gacctctccg acgtcgttgc
      661 agatcggaaa catgaggcgg ccgcgaaacc gatcgtagac ttcgctttca ggtcgctctt
      721 cgtcgcgcag cttgacgagg ccactttgca aaattgtgcc gcgcttatag ccctggtcga
      781 gcgcccactt taaacaggca tcccaactct ccggcgcaaa gccgagctgc cacgacttca
      841 ccaccgcgct gctgatcccg cgctgcttca agtaatcgcg cgctggggcg gcgaactctc
      901 gtttcagcag gttttcgtga aaccattccg ccgcttccgc gtgcaatctc aggagcgtcc
      961 gccgcgcttc gtgctgccga tcgtcctccg cgctgccgcc tcgttcctcg atcaccggaa
     1021 taccggcgcg agcggcgagg cggcgcacgg aggccggaaa atcgatgtgc tcgtaatcca
     1081 tcacgaagcg gaaaacactc ccgccgacgc cgcagccgaa gcagtgaaac gattgccgcg
     1141 ccgggttgac gtggaacgac ggcgttttct cctgatgaaa cggacagagc gcgcggaaat
     1201 ttgcgcccgc gcgtttgaga gggaaatagg aaccgatcac ctccacgatg tcgttcgcgg
     1261 cggcaacttg ttcgatagtg tggttcggga tcgttcccat gcgcgctctt tttcctcgtg
     1321 acgactaccg atcactgcca gattaatgca gagcccatct caagtcatgt cgagcgaagt
     1381 cgagacatcc cgtggcgtaa cgcgccctgc cgccagaacc ttccgtcaac caaccatgct
     1441 gcgtattctc atcatctttc tggtcgccac tttgtcgtta ggcgcagccg cggccggcga
     1501 agtgatcgcc ccgggcagcg tggtggtcgc cccgttgcat ggcgaggttt cgccctcgct
     1561 cgcgcttttc ctccggcgcg cgcaaaaggc ggccgagaac gatggcgcga ccgcgatcgt
     1621 cctcgacatg aatacttatg gcggggagct cgattcggcg gagcaaatca ctgacatcct
     1681 gaatcacgcc acgatcccga cctacaccta catcaacacc aatgccggtt cggccggtgc
     1741 gatcatcgcg ctttccacca ggcacatcta catggcgccg gtcagcgcga tcggcgccgc
     1801 tgccccgatc ctctcgaccg gcgaagatct tccgccgacg caaaaggaga agacggtctc
     1861 ctactggtcg gcgctcgtcc gcggcgcggc gctgaagaat ggccacaacc ccgacatcgc
     1921 cgaagccttc atcaacaagg agaaagaagt gaggatcggc gaccgggtga ttcatccgaa
     1981 aggctcgctc ctcaccctca atgcgcagga tgcgaccgaa aaaattaacg ggaaaccggt
     2041 gctggccgag ggcgttgccg attcattgca cgagctaatg cagaaggcgg gcttgaaagg
     2101 tgaggtggtc gagttcgacc cgagcggttt cgagcaactc gccttttggc tcaccgccct
     2161 ctcgcctctc ttattgttag gcggaatcct gggcgcgtat ctcgagttca agattcccgg
     2221 gttcgggttg gcgggaatcg tctccgtcgt ctgttttgct ctcttctttt tcggccacta
     2281 tttcgccggg ctcgccggtt gggaagtggt cgggcttttc gttctcgggg tcttcttcgt
     2341 cctcgtggag gttttgtttt ttgggcactc gacgattgtc tttggggtca tcggagtgct
     2401 cttgatgttc gcctcgctcc tttgggcgat gatcgatcgg tatccgggcc agaaacttgt
     2461 cccgaccgga caaatgctaa gaattccgct gctcaatatg ttgctgacct tcgccgccgc
     2521 cgcggtcctg atcgtgctcc tcgcccgctt tctcccgcag acgagtatct atcgccgctt
     2581 cattctctcg accaccaatc cgccgggtcc ttcgcttgcg ggcgcgccgc gcgcgtttgc
     2641 caccgccctc ccactcacgc ccggaatgcg gggacggtcc ctaacgattc tccggccgag
     2701 cggcaaggcg gaattcgaga gcaatgtggt ggacgtcgtc acagaagggg aattcatcgc
     2761 gccggaaacg ccggtcgcga tcgtctccac tgacgggatg cgggtcgtgg tccggagaat
     2821 cgctgcctga ggtcgcgccg tgtcgttacg ccgcatccgt tgcggctgcg cgtccaggac
     2881 atgcggaagc atgtccctcc gggtcgcgct ccgagcgaag taatttcttc cctcctcctt
     2941 ccgccttctt cctttcgaaa atgcgcccgg cgcggctcga acgcgcaacc tacagcttcg
     3001 gaggctgtca ctctatccag ttgagctacg ggcgcagcga gcgtgattat aacggccgcg
     3061 tctgaaagca caacccgagg ccccttcccc gaacccttta caattcagtg cagaagcgct
     3121 aggtattcga ccccgtttcc agagcagaaa gcgactttat gaacatcatc gacaccatcg
     3181 ccaaagagca gcagaagacc gacgtcacga aattcaacgt gggcgatggg gtgcgcgtgc
     3241 acacccgcgt gcgcgaaggc gacaaggaac gcatccagat cttttccggc atcgtgatcg
     3301 gccgcaaggg gaccggcttg aaccagacgt ttaccgtccg ccgcatttcc tacggtgagg
     3361 gcgtggagcg cgttttcccg gtgcattccc cgcgcatcgc gaaggtggaa gtggaaaagc
     3421 agggtcgcgc ccgccgggcc aagctcaatt atctgcgcaa tcgcaagggc aaggaagcga
     3481 cggcggtcag ggaatagcga cgagtgacga gtgaatggtg gaaacgccat ccattcacga
     3541 ttgactgaac acaatttact ttttcgattt cacccacgac gtcggattgc gtaaagtgcg
     3601 caacgatgta tcgccgctgc ggtttccggc atgaaaaaaa actgcggtcg ttaggcatcg
     3661 gctgcgttgc cgggatcgat gaagcggggc gtggtgcgct cgccggcccg gtggtcgcgg
     3721 cggcggcgat cttgccggaa cgttttcggc accggaaatt aaacgattcg aaacagcttt
     3781 cgcctgagct gcgtgaagaa atttacgaat acctgacgaa tcactccgag atcgtttggg
     3841 cggtcgggat cgtcgattcc gtcgagattg accggatcaa tatcctgcgc gcatcccacc
     3901 aggcgatgcg aatcgcggtc gtcggcctga cggtttcgcc ggagcacgtc ttgatcgatg
     3961 ggctgccggt ttttccattt ccccttccgc aaacagcgat cgttgatggc gattgcctca
     4021 gtctgagcat cgcggcggcg agtgtgatcg cgaaggtgac gcgcgacacg ttgatgcggg
     4081 aattttgcgc gcgtttcccg cagtattgct tcagtcagca caagggatac ggaaccgaac
     4141 tgcatctcca gaaacttcat gaacatgggc cttgtccaat tcaccgccga tcgtttgagc
     4201 cggtggcgca gccggttttt tccttctagc tccgggcacc tgcggctcgg ggtgcgcggc
     4261 gagaaactgg ccgcgcgtca tctgcgcggc cacggtttca agatccttta ccgcaatttc
     4321 cgtgggcgcc agggcggcga gatcgatctc gtctgccggg agcgcgacac ccttgttttt
     4381 gtggaggtga aaacccggac ccgcgaggat ttcgggcggc cgctggaggc ggtcaatcgc
     4441 cggaaacaac gccgcatttc gttaggcggg ctggcctggt tgcgattact gggcaacccc
     4501 gacatcctgt ttcgcttcga tgtggtggaa gtcacgatgg cggatgagac ggcgccccgg
     4561 gtcgagctga ttcggaacgc gtttcagttg tcggatccgt atatttactg aagagcgcgc
     4621 accaccggcc gcgccgcctg cgcgaaccgc acaaccaaca atccggcgaa cgccacccca
     4681 gcgccttttg ccgccgtttt ttttgtgatg tcgtttcggt aatttttctg ctacacccct
     4741 cccatgacta cagcgaatcg aatgggtcgc ggtgggaaaa ctgcgactgg actggcaggg
     4801 aaatgcgcct cggcgatggc ggtcgttagg agcggccggg aaatggttca tttggcggcg
     4861 ggagcatttg tcctgtccgc agtcttgttt tctggagcgg cgagcaaggg ggctaccacg
     4921 gaagttatct actcctttgc gggagaagac gacggcgaat acaccgacac ggatgtggcg
     4981 atcgacgcag caggaaatct ctacgggacg tccgtcctgg gtggagaatt tggcggcggc
     5041 acggtctggc gactgtcgcc ggtcggtgat agctgggtgc ataccgttct ctacagtttc
     5101 accggcggcg cggatggtgg tgagccctac aaaggagtta cgctcgacgc ggcgggcaat
     5161 ctctacggca ctgccgtgac cggcggctcg ggaagttgtg agggcggatg cggggtcgcc
     5221 tacaagttga ccaaatccag cgacacttgg acacaaagtg tcatccacgc ctttaccggg
     5281 gaagatggct ctggtcctgg agcgagggtc gtggtcgacg atcgcggcaa cgtttacgga
     5341 acgacaccga ccggtggagc aaacggatta ggggtgatct acgcgcttca ttccaatgcg
     5401 aatggcgatt ggatgctgcg agtcattcac actttcaccg gtggatccga cggatcatcg
     5461 ggctcagctg gccagttagt tccccgtggt gggcatgttt acggtgcggc gaccaccgga
     5521 ggggtggatg gtgccggcgt catcttcgaa ctgaccccga cgcaaatcgg cgagtggggc
     5581 tttaggacca tttattcctt ccaaggcgct cccgatggga gctttcccta cggcgctttg
     5641 cttttcgatc ccgcgggtaa tctctacggt acgacttatt atggcgggac aaatggtttg
     5701 ggcgcagttt acaaattgag tctcactggg accagggaat ggagcgaaac ggtgctctac
     5761 agcttccaaa ctgggagtga tggaaacagt tcaattggta atcttgtggc tgacgcagca
     5821 gggaatcttt acggcacgac cagtgaaggt ggattgggaa gcggcacgat tttcgaactg
     5881 actccgggcc agggcgggac atggaccgag agtttgcctc acagctttca aggcccgcct
     5941 gatggagctt ttccctatgc cggcatggtc ggtgacggcg caggaaactt ttacggcgcc
     6001 accgttcatg ggggcgatgc tgatggcgac ggcgcgattt acaagtttac gccttagact
     6061 tcgcggagag cgcgatttca gaaggacgtg cttccgcgcg tccattcccg tcacagacca
     6121 gacgtgcgga agcactccct ccggacgcgt gaggacgacg aagtttcttg agcggtgggg
     6181 cgggccaagc tcctatgaag catggcgcgc gcgcgaaaat ccagttggtc gctaacgctg
     6241 ctcgatttgt tgcttgttgc cgggccagtg gcgatcgtgc tgcgctacgt gccggcgtgg
     6301 aggaatgaaa cccttctctt cttcgtcgcg gcgatcgcga tcgtgccgtt
//
LOCUS       DATMNS010000104        12922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4014042, whole genome shotgun sequence.
ACCESSION   DATMNS010000104 DATMNS010000000
VERSION     DATMNS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 12922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12922
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4014042"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VNW28_03740"
     CDS             <1..447
                     /locus_tag="VNW28_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09067.1"
                     /translation="ALYLYYTEQRDTAGTYRVLGKLVELLPDDLSVRNNFILISLLLQ
                     TEPTRMRTMARELNQKEPGNGSFASTYAFALYQTGDTEGALRVFNTLSPEQLRDPAIA
                     LYYGVILAGAGHKQEAAAALAKTSGAKLLPEERALVESVRAKLAQQ"
     gene            complement(645..1583)
                     /locus_tag="VNW28_03745"
     CDS             complement(645..1583)
                     /locus_tag="VNW28_03745"
                     /inference="COORDINATES: protein motif:HMM:NF042846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding domain-containing protein"
                     /protein_id="HXA09068.1"
                     /translation="MIGLLKPILMTLINKYIGAMAVAGLMAVGFGGPLQASIVDLGAA
                     GQFGALALTTGIDDSGPLGPDGSVTINADVGVASSGQSFSASGSVVYSGNLYLHTGVT
                     YNNSAPGVPLPQPQNATNDAFLAQARSDAFAASNFALTLGVTATYGTINTNTTISEGA
                     VGNYVFSLQGGINFSGNSTLTLSAPAGSCYVLNISNQFVLTGGSVLLSGGLTADNVLL
                     NYTGTSDVRFSGGGNSSQVYGTILAPNAKVDLSPGLVVGRVIASGLSMSSGAQILTPV
                     PEVTPGSVIFGFLGLVIAVGSRKALAARLIRVRAKQ"
     gene            1777..3348
                     /locus_tag="VNW28_03750"
     CDS             1777..3348
                     /locus_tag="VNW28_03750"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HXA09069.1"
                     /translation="MANLTAEAGEPARAGVATTRDSRGPIVLMALAFFFLWLEVVHQL
                     QGEWSFNPQYSYGWTVPFLAAFLFFQRWSRRPAPGATHFRFLTITLMVLAAGALFPAR
                     LIAVANPDWRLLDWMMTLAAVVISLGALQLAGGERWLRHFAFPILFFLVATPWPTQFE
                     QFVVQHLMRADAAITIQVLNLAGIVAIQHGNVIELSTGQVGIDDACTGVRSLQATFMI
                     SLFLGEFYWMRLRQRFLLIVAAGGLAFFCNLARTFVLCEVAANSGIAAIHRWHDPAGL
                     SIFFICLFLLWGMSFWLRPDHSAEPALPVPFHFTSVRFPLALLAWFALTEIAVATWYR
                     PGLAQIASAPWTIAWPTSELDYQPQPVAAAAEVLLQSSDGGAATWRSSNNDRWMMYSF
                     HWLPGRTAALFLKNHRPDICLPASGLTMVRETGIHLLTINGINLPIRSYRFDDNGQPL
                     HIFYCYWNGRSSYVSDASATTEDWTARGRLHAAWQGKRELGARMFELAVWGHENDAQA
                     TAALQRELEKIVRPH"
     gene            complement(3415..5757)
                     /gene="pnp"
                     /locus_tag="VNW28_03755"
     CDS             complement(3415..5757)
                     /gene="pnp"
                     /locus_tag="VNW28_03755"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXA09070.1"
                     /translation="MQHKVTAQIGSTQINIETGKIAKLADGSVIVSCGETIVLTSAVS
                     ATSLKEGQDWFPLTVDYREKAAAVGKFPGGYFKREGRPSEKETLTSRMTDRPLRPLFP
                     SGYLYDTQIISILLSADGENDPDILAINGASAALCVSDIPFKGPIGAVRVGRVNGEFI
                     ANPTHVEREQSDLDLVYVGTENDVIMIEGAADELPEEDFIKSLEFAHGHAREMIRIQK
                     ELAAQVGKPKREMPLLEVQPEILEIADRVAGDRIEGALYTEGKVARSKAVSGLRDEVK
                     AAVLEKFPTADKFAINQAFDYVQKKSFRSNILDKQKRVDGRGYDDLRKITCEVGVLPR
                     AHGSAIFQRGETQAMALATLAPIEEAQNIDAYGGGETSKSFLLHYNFPPFSVGETGRF
                     GGASRREIGHGALAERSVEPVVPNQEDFRYAIRITSEVMESNGSTSMASVCSGMLALM
                     DAGVPIKTPVAGISVGLVTENDESGNLKRYNLLTDIIGSEDHFGDMDFKLCGTKDGVT
                     GFQLDLKLPGVSHKILGEAIHRAKDARTRILAIMAETLAQPREQLSKYAPRIETIKIN
                     PEKIGALIGPGGKTIKGIVAETGAEINIEDDGSVHIYATSGESMARAKEIIGGMTKEI
                     EVGQTYHGRVVSIKEFGAFVEVFPGKDGLVHISELADFRVNRTEDVAKIGEMIWVKCI
                     GIDDKGRVKLSRKAALKERGEIETGVPMGDAGSGQGESRGEYRGGDHGDRGGRRDRGE
                     RRPGGRGRGPDRRRDEPRAAGEPPPASPAQSQPEDYEPSR"
     gene            complement(5949..6212)
                     /gene="rpsO"
                     /locus_tag="VNW28_03760"
     CDS             complement(5949..6212)
                     /gene="rpsO"
                     /locus_tag="VNW28_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HXA09071.1"
                     /translation="MAESSDTMDMKEFQLHEKDTGSADVQVALLTRRISQLTEHLKSH
                     AKDHSSRRGLLKMVATRRSLLDYLSRSKTDRYKSVIEKLNLRK"
     gene            complement(6313..6759)
                     /locus_tag="VNW28_03765"
     CDS             complement(6313..6759)
                     /locus_tag="VNW28_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09072.1"
                     /translation="MEGLPKFGIIVVGACASSALALWLFRGGEPALRAVNKQAHSQKP
                     VPGGVEIRETFTSRMPSGKGVAVVANTGEFDGNHVLVAVPDAFHQVHYLPGTDPQAAG
                     YVTKMRAAHQQMAPDYHPSNADLSAVPAGKSVSSNRKQVGVDAGKK"
     gene            complement(6772..8493)
                     /locus_tag="VNW28_03770"
     CDS             complement(6772..8493)
                     /locus_tag="VNW28_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09073.1"
                     /translation="MTRIRLERPVARRSKPVETAAKRAFRSEEGNALVVAIFVVAILC
                     IGLGTHLASLSSYRRLQEQRILDDRAMMAAEAGLASQIAWLESQPTPPSADVTQSIAL
                     ASGQFAPFQNVTTVIHVQTLNGQIYWTITATPSCDITATRNASVFKRVQATISQQNFA
                     RYERFINDYGPVWTPGYLYGEGLNTVYMGPVNINSGCGFFTNFWSLSEVTSAAPGGVR
                     TFADWGSYIAGVYGLPDSSNYINVLEYMSPTYPNAPQFYGGLRVLPAPISLPTDMNVD
                     VRAQQLRDHAGLTLPDNYPGYLPSAGPNFVIDLSNPGGSDGQITVRQYLGTVSGAPAY
                     GPPQVTTVSGVNGAMIVEGNVVSLQGVLNGRLTIGVFASTDLPTGGNVNITGDVQYQS
                     RMSNTGFEYTDAPGLYTADGSGINQSYVEQLQAQLNSVTDILGIVAEGNVLVMETDLN
                     GNPIANDPTKPIHLDAVVMATGASASTPGGGGFGVEGNLTRPPGHAYFLGGMIENKQV
                     DWALYSTSGILNGIALTQLWDQRASEPGGAPPFYPTTGTFELVPNTWNATYVQSANAP
                     IVYPPLP"
     gene            complement(8519..9115)
                     /locus_tag="VNW28_03775"
     CDS             complement(8519..9115)
                     /locus_tag="VNW28_03775"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXA09074.1"
                     /translation="MGRAAEDYRETQAPFRGVFTGGFTLIEVLIASAIGSIIGLISIG
                     GLVEGLHLFKSNSTEMIARDQGSRAVRKMTADIQQSVLAQIFPTYQGTTGAAGDYGSC
                     VVLQESSGGSVAYYLYAPTSDPNSGGIYYAANGAVAPNPLTDKLLVSTVQDLDFRRDV
                     NGSIRVGFDIGTFGFPTLLVGAKEADLVRFTTSSLPRN"
     gene            complement(9115..9699)
                     /locus_tag="VNW28_03780"
     CDS             complement(9115..9699)
                     /locus_tag="VNW28_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXA09075.1"
                     /translation="MDSNCAALIVPAQQMGRPRMALDRSASGMTLVEVMVAVAVLTIS
                     LIGTMAAISWMRMENRTASQRLLVASIGAEMIDLFKSLQYTDIHNSTAGVPIYLKGFG
                     SASPNLAWVVPQAGQWQSLPVEAVNSSSASAPNLVPDKIPQGQWKVEFVPDAVIPQLQ
                     QINITIQWQLYAGSTRPPLSYAISTRVCGDFPKL"
     gene            complement(9844..10446)
                     /locus_tag="VNW28_03785"
     CDS             complement(9844..10446)
                     /locus_tag="VNW28_03785"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXA09076.1"
                     /translation="MIRFFFKLIICFLLAAGAGIGLLALRSGDPFYTLYEWISPARFQ
                     QYDQLIHVVSAANGLDPMLVKAVVWRESRFDAHKLGAAGERGLMQVSEIAARDWSKEN
                     RTENFRVEELFDPKRNLEVGAWYLHRTMLHWRQARDPTPFALAEYNAGASRAQRWAGG
                     DGSRPISASAFQRNIDFPGTRKYVESILERYRFYQRRGRM"
     gene            10567..12792
                     /gene="priA"
                     /locus_tag="VNW28_03790"
     CDS             10567..12792
                     /gene="priA"
                     /locus_tag="VNW28_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HXA09077.1"
                     /translation="MSAFARVIIDRSIRRELDYSVPEELAGRVVVGSRVRVPFRDKWA
                     LATVVDTLDETPAEGVRPIEALLGDRPVLSACLIELARWMSTYYCCPVETVMRSLLPQ
                     VIRRAEVGWKKQLFVHPARIAPTEEIEKLRKRAPRQAELLDAIAQLPAPIPAADLLRQ
                     TSLDNSTLRALVRRGFVELREQAVARDPHGTEEFIADTELAMNEEQTAALKLIETALK
                     NPETHPPILLHGVTGSGKTEVYLQAIRATLARGRTAIVLVPEISLTPQTVERFKSRFA
                     AQPDMVAVLHSHLSEGERHDEWHKLHAGRARIVIGARSAVFAPLENLGLIVVDEEHET
                     SYKQEEAPRYHARDVAVVRAKIEKCVTVLGTATPSLESYANATRGKYVLSPLTQRVDQ
                     CQMPLIRIVDLRQERRKEKAAAILSEKLRSAIGNRLEKHEQTILFLNRRGFSTSLLCS
                     HCGKARDCPNCSVALTFHRSNARLSCHLCGHSAAVPQRCPECGQDALIYAGFGTEKVE
                     ANVSAIFPKAVVRRMDADSMTRKDAYRETLHAFRSGKIDILVGTQMIAKGLHFPNVTL
                     VGIINADLALHLPDFRAGERTFQLLTQVAGRAGRGEAPGEVFVQTYTPFSPSIQFARH
                     HDFAGYFEQELEFRERCDFPPFKHAVLITVRSEHETHASFSAETLARRLREALPEEFT
                     LGAPAPAPLEKLQGHYRFHILLRGEAILRLTRLIRETLDKLPFPEDVAVAVDVDPYQL
                     L"
BASE COUNT         2516 a         3947 c         3802 g         2657 t
ORIGIN      
        1 gccctctatc tttactacac ggaacagcgc gataccgcgg gcacataccg ggtcctgggc
       61 aaactggtgg agctgttgcc ggatgatctt tctgtcagaa acaatttcat cctgatctct
      121 cttcttcttc agacagagcc gacgagaatg cggaccatgg cgcgggaatt gaatcagaag
      181 gaaccaggca atggcagctt cgcttccact tacgcttttg cgctctatca gactggcgac
      241 acggagggcg cacttcgcgt ctttaacacc ctctccccgg agcaactgcg cgaccccgcg
      301 attgccttat actacggagt gattctggcc ggagcggggc acaagcagga agcggccgcg
      361 gcactcgcga aaaccagcgg cgcgaaattg ctgccggagg aaagggcgct cgtggaaagt
      421 gtgcgggcca aactcgctca gcagtaggtt cttcactcgt cacgaaagcg cgggagcaaa
      481 gggttgggtc ccctggaggt tgatgctctg gaggtagtca aatcagtagc cggcgcgtta
      541 accggcgtcg cataagctcc acgccacagg acggactatt aaggaaaaaa gcggcgccgg
      601 aaacccggcg ccgctcccaa accagtcgaa aaaaacctgg cggcctattg tttggcccgc
      661 actctgatga gacgcgcagc caaggctttg cgtgagccga cggcaattac cagacctaaa
      721 aacccgaaga tcactgagcc cggagtgact tccggtaccg gtgtcagaat ctgggctccg
      781 gagctcatgc tgagtcccga ggcgatcacc ctgcccacga ccaagccggg tgaaaggtcc
      841 accttagcgt ttggcgcgag gatagtgccg tagacttggg aactgtttcc gcccccggag
      901 aaccgcacgt cacttgtgcc cgtatagttc aacaaaacat tgtccgcggt caggccgcca
      961 gaaagaagga cgctgccgcc cgtgagcaca aattgattgg agatatttaa gacatagcag
     1021 ctgccagccg gagcactcag cgtcagggtg ctattgccgg agaagttgat ccccccttga
     1081 aggctaaaca catagttccc gaccgcgcct tccgatatgg tggtgttcgt gttgatcgtg
     1141 ccataggttg ccgtcacccc cagggtgaga gcgaaattgg aggccgcgaa agcatcggag
     1201 cgagcctgcg cgaggaaggc gtcgttcgtc gcgttttgag gctgcggcag cgggacaccc
     1261 ggggccgagt tattgtacgt gacgcctgtg tgcaggtaga gattcccgga gtaaacaacg
     1321 ctgcccgagg ccgagaacga ttgtccgctg gaggcaacac cgacatcagc gttaatcgtg
     1381 acggacccat cgggtccgag cggaccagaa tcgtcgattc cagtagtaag ggcgagcgcc
     1441 ccgaactgac cggcggcacc caaatcgacg atcgaagcct ggagcggccc tccgaagccc
     1501 actgccatga gacccgccac tgccatcgcc ccgatgtatt tattgattag agtcataaga
     1561 attggtttca acaagccgat catggctaca attgagtctg agcgtcaata taaatttttg
     1621 tcggccgcac tttctttcag cttcgccggg cctcccctcg aaaaatccag cggaaatcca
     1681 ccatgccggg gacgacccga attccaaatt ggcggccagc ggcattcaca cgcgccacga
     1741 tggcgcagag gcgcataaac tgctaaaaca tccggcatgg ccaatttgac cgcggaagcc
     1801 ggggagcccg cgagggccgg cgttgcgacg acgagagatt cccgcggacc gatcgttttg
     1861 atggctctcg cctttttctt tctctggctg gaggttgttc accaactgca aggggaatgg
     1921 tcgttcaatc cgcagtacag ctacggctgg acggtgcctt ttctggcggc ctttctcttt
     1981 ttccagcgct ggtcccggcg ccccgctccg ggcgcgacgc acttccggtt cctcacgatc
     2041 acgcttatgg ttttggccgc aggcgcgctc tttccggcgc gcctcatcgc ggtcgccaac
     2101 ccggattggc gcctgctcga ttggatgatg acgctggccg ccgtcgtgat ttcgttaggc
     2161 gcgctgcaac ttgcgggagg cgagcgctgg ctccggcact ttgctttccc catcctcttc
     2221 tttctcgtcg ccaccccctg gcccactcag ttcgagcaat ttgtcgtgca acacctgatg
     2281 cgcgcggacg cagcgattac catccaggtt ttgaacctgg cgggcatcgt cgcgatccag
     2341 cacggcaacg tcatcgagct gagcaccgga caggtgggga ttgacgatgc ctgcaccgga
     2401 gtgcgttctc tgcaggccac ttttatgatt tcgctgttcc tcggcgaatt ctactggatg
     2461 cggctgcgcc agcgtttcct cctcatcgtc gccgcgggag ggcttgcctt tttctgcaac
     2521 ctggcgcgca cttttgtgct ctgcgaagta gcggccaact ccggaatagc tgcgatccat
     2581 cgctggcatg acccggccgg actttcgatc tttttcatct gcctttttct tctttgggga
     2641 atgagtttct ggttaagacc tgatcattcc gcagagcccg ctcttcccgt cccgtttcat
     2701 tttaccagtg tccgcttccc gctcgctctc ctcgcgtggt ttgccctgac cgaaatcgcc
     2761 gtcgcaacct ggtaccgccc gggcctcgcg caaatcgcga gcgctccgtg gacaattgcc
     2821 tggccgacga gcgaactcga ctatcaaccg cagcccgtcg cggccgcggc cgaggtgctt
     2881 ctccaatcta gcgatggtgg ggcagccacc tggaggtcat cgaacaacga ccgctggatg
     2941 atgtattctt tccattggtt gcctggacgc acggcggcgc tcttcctcaa aaatcatcgc
     3001 cccgacattt gtctgccggc cagcggccta acgatggtgc gggaaaccgg cattcatttg
     3061 ctcacgatca acggcatcaa cctcccgatt cgctcctatc gcttcgatga caacggacag
     3121 cctctgcata ttttctattg ttactggaac ggtcgttcgt cctacgtgag cgacgcgtcc
     3181 gccacgaccg aagactggac cgcgcgcggt cgcttgcacg ccgcttggca gggaaaacgc
     3241 gaactcggcg cgcgcatgtt cgagctcgcg gtttggggcc acgaaaacga cgcgcaagca
     3301 acggcggcgc tccagcggga actcgaaaaa atcgtccgcc cacactgagt ttggacgctt
     3361 gtgaaacccg ccggagacgt ctctctccgg tttccaaacc agcagcccgg acggttaacg
     3421 agagggctcg tagtcttccg gttgcgattg cgctggtgag gccggcggcg gctcgccggc
     3481 tgcgcgcggt tcgtcgcgac gccggtcggg tccacggccg cgaccgccag gacgacgctc
     3541 cccacggtcg cgccgtccac cacggtcgcc atgatcgcca ccgcgatatt cgccacggct
     3601 ttcgccttga ccagaaccgg catcacccat gggtacgccg gtctcgattt cgccgcgctc
     3661 tttgagagcc gctttgcgcg agagcttcac tcgccccttg tcgtcgatgc cgatgcactt
     3721 cacccaaatc atctcgccga tttttgcgac atcttccgtc cggttgaccc ggaaatcggc
     3781 cagttcggaa atgtggacca agccatcctt gccagggaaa acttcaacaa acgcgccgaa
     3841 ttctttgatc gaaacgaccc ggccgtgata ggtctggccg acttcgattt ccttcgtcat
     3901 gccgccgatg atttccttcg cgcgcgccat gctttcgccg ctcgtcgcgt agatgtgcac
     3961 ggagccgtcg tcttcgatgt taatctccgc gccggtctcg gccacgatcc ccttgatcgt
     4021 cttgccgccg ggtccgatga gcgcgccgat tttctcgggg ttgattttga tcgtctcgat
     4081 ccgcggcgcg tatttgctca gctgttcgcg cggctgggcg agggtctcgg ccatgatggc
     4141 gagaatcctg gttcgcgcat ctttcgcgcg atgaatcgct tcgcccagaa tcttgtggct
     4201 cacgccggga agtttcaggt cgagctgaaa gcccgtgacg ccatccttcg ttccgcagag
     4261 tttgaaatcc atgtctccga aatggtcctc ggagccgatg atgtcggtta gtaagttgta
     4321 gcgcttcagg ttgccgcttt cgtcgttctc cgtcacgaga ccgacggaaa tgccggcgac
     4381 aggcgttttg atgggaacgc ccgcgtccat cagcgcgagc atgccggagc aaacgctcgc
     4441 catggacgtt gagccgttgg actccatcac ttcgctcgta atgcggatgg cataacgaaa
     4501 atcttcctga ttcggcacga ccggctcgac cgagcgctcg gccagggcgc cgtggccgat
     4561 ctcgcgccgg ctcgcaccgc caaagcggcc ggtttcgccg acgctgaagg gcgggaaatt
     4621 gtagtgcaaa aggaagctct tcgaggtctc gccgccaccg taagcgtcga tgttctgcgc
     4681 ttcttcgatc ggtgcgaggg tcgcaagcgc catcgcctgc gtttcgccac gctggaaaat
     4741 ggccgagcca tgcgcgcgcg gcagcacgcc gacttcgcag gtgatcttgc gcaggtcatc
     4801 gtagccgcga ccgtcgaccc gcttctgctt gtcgaggatg ttgctccgga acgacttttt
     4861 ctggacgtaa tcgaacgcct ggttgatcgc aaatttgtcc gcggtcggga atttttcgag
     4921 cacggccgcc ttcacttcgt cgcgcaaacc gctgacggct ttgctgcgcg ccactttgcc
     4981 ttcggtatag agcgcgccct cgatgcgatc gcccgccacg cggtcggcga tctcgaggat
     5041 ttctggctgc acttcgagca acggcatctc gcgtttcggc ttgccaactt gggcggcgag
     5101 ttccttttgg atgcgaatca tttcgcgcgc gtgcccatga gcgaactcga gggacttgat
     5161 gaaatcctct tccggcaatt cgtccgccgc gccttcgatc atgatgacat cgttctccgt
     5221 gccgacgtaa accagatcga gatcgctctg ctcgcgctca acgtgggtcg ggttcgcgat
     5281 gaattctcca ttcacacgcc caacccgcac cgccccgata gggcccttga agggaatgtc
     5341 ggaaacgcag agcgcggccg aggcaccgtt gatcgcgaga atatcggggt cgttttcgcc
     5401 atccgcgctg agcagaatcg agatgatctg cgtgtcgtaa agatagccag acggaaaaag
     5461 tggccgcagc ggccggtcgg tcatgcgtga agtgagcgtt tctttttcgc ttgggcgacc
     5521 ttctcgcttg aaatagccgc ccgggaattt cccgaccgcc gccgctttct cgcgataatc
     5581 gacggtgagc ggaaaccagt cctggccttc cttgagtgag gtggccgaga cggcgctggt
     5641 gaggacgatg gtttcgccgc aggagacgat aacggagccg tcggcgagtt tggcaatttt
     5701 gccggtttcg atgttgattt gcgtggaccc gatttgggcc gtgaccttgt gttgcattac
     5761 ttctttctgg atgtgcgaaa tccaggccgg tcaccctgat tgtaggtttt aaccccgccg
     5821 cggcggcggg actaaaacct aaactcaagg tgcactgacg gcctggtcgc cggttgtgtt
     5881 cttggtttcg cgcatgactc gagcgggcgc gcgaggaacg cgagtggcgc cagagattgg
     5941 accgcggatt atttccgcag gttcaacttc tcgattacgc tcttgtagcg gtcggtcttc
     6001 gaccggctca aataatcgag gaggcttcgg cgcgtggcga ccatcttgag gaggccgcgg
     6061 cgcgaggaat gatctttggc gtgactcttc aggtgttccg tgagctgcga gatccggcgg
     6121 gtcaaaagtg caacctgcac atcggcgctg ccggtgtctt tctcgtggag ctggaactcc
     6181 ttcatgtcca tcgtgtcact gctttctgcc ataaacgaag gcccagtgta aatgaagttt
     6241 cccgaatcgc aagtcgtgcc tcccgcgggc ggtcgctcct gtcatcacac tgcggagcat
     6301 cctgccgttt gcctacttct tacctgcgtc cactccaact tgtttccgat tgctggaaac
     6361 ggacttcccc gcgggcacgg cgctgagatc ggcgttgctc ggatggtaat cgggagccat
     6421 ttgttggtgc gcggcgcgca tcttagtgac gtatcccgct gcctgggggt cggttccggg
     6481 gagataatgg acttggtgga atgcatcggg caccgccacg agaacgtgat ttccgtcgaa
     6541 ttcgccggtg ttcgcgacga ccgccactcc ctttccagac ggcatccggg aagtaaaggt
     6601 ctcgcggatc tcgacgccgc ctggcactgg tttttgcgaa tgggcctgct tgttgaccgc
     6661 tcgcagcgca ggctcgccgc cacgaaagag ccataacgcc agggccgaac ttgcacaggc
     6721 tcccacgacg atgatgccga attttggtag cccttccatg aaaaaattta gtcagggaag
     6781 gggcgggtaa acgatcgggg cattcgcgct ttgcacataa gtcgcattcc aggtgtttgg
     6841 aaccagttcg aaggtcccag tcgtcgggta gaaaggcgga gcgccgccag gctccgacgc
     6901 gcgctggtcc cagagttgcg tgagggcgat tccattgagg attccggagg tgctatataa
     6961 ggcccagtcg acttgtttgt tctcgatcat tccgccgagg aagtaagcgt gccccggcgg
     7021 acgggtaaga tttccttcca ccccgaagcc gccgccgccg ggcgttgagg cgctggctcc
     7081 cgtggccatg acgaccgcgt cgagatggat gggtttcgtc ggatcattcg cgatcggatt
     7141 tccattgagg tcggtctcca tcaccagcac gtttccctcg gcgacaatcc cgagaatatc
     7201 agtcacgctg ttaagttgcg cctggagctg ttcgacgtag gactggttga tcccgctgcc
     7261 atcggccgtg tagagtccgg gcgcgtcggt gtattcaaag cccgtattgc tcatgcggga
     7321 ctggtactga acatcgcccg tgatgttgac gttcccgccg gtgggaaggt cggtgctcgc
     7381 aaatactccg atcgtgagcc gaccgttgag aacaccttgc agcgaaacca cgtttccctc
     7441 gacgatcatg gctccattga cacccgaaac ggtcgttacc tggggaggcc cgtaggcggg
     7501 agctccactg acggttccga ggtattgccg cacggtgatc tggccatcgc tgccgcccgg
     7561 atttgacaaa tcgatgacga aatttggacc ggccgatggc aagtagcccg gatagttatc
     7621 cggcagggta agacccgcgt ggtcgcgcag ttgctgggcg cgcacatcga cgttcatgtc
     7681 agtgggcagt gagatagggg cgggcaaaac gcgcaagcca ccgtaaaact ggggagcgtt
     7741 gggataagtc gggctcatgt actccaggac attgatgtag ttgcttgagt cgggcaggcc
     7801 atagaccccg gcaatgtaac tcccccaatc ggcgaacgtc ctcactcccc ccggcgcggc
     7861 ggaagtgact tcgctcaaac tccaaaaatt tgtaaagaaa ccacatcccg aattgatatt
     7921 cacggggccc atgtagaccg tgttgaggcc ctcgccgtag aggtaaccag gggtccagac
     7981 agggccgtag tcgttgatga agcgctcgta tctcgcaaaa ttctgttggg aaatcgtcgc
     8041 ctgcaccctc ttgaaaacgg aagcattgcg cgtcgccgtg atatcgcagc ttggagttgc
     8101 cgtgatcgtc caatagatct gtccgttcaa ggtctgcacg tgaatgacgg tcgtgacgtt
     8161 ctgaaagggg gcgaattggc cactggccag cgcgatggat tgagtcacat ccgcgctcgg
     8221 aggagtgggc tggctctcga gccacgcgat ttgcgatgcc aggccggcct cggccgccat
     8281 catcgcccgg tcgtccagga ttcgttgctc ctgaagacgc cggtaactgc tcaggctggc
     8341 gagatgcgtt cccaatccaa tgcagagaat cgcgacgaca aagatggcca cgaccaaagc
     8401 attgccttcc tcactccgaa aggcgcgctt ggccgcggtc tccaccggtt ttgagcgcct
     8461 ggcaactggt ctctcgagac ggattctggt catggggatt taatgaccga tgtgcttctt
     8521 aattccgcgg aaggctgctg gtcgtaaatc gcaccaggtc ggcctctttc gcaccgacca
     8581 acaaggtcgg aaagccgaag gttccgatat cgaagccgac ccgaatcgag ccattcacat
     8641 cacgtcgaaa atccaagtcc tgcacagtgc ttaccagcag cttgtcggtc aacggattgg
     8701 gcgcgaccgc accgttggca gcataataga tcccaccgga gttcggatcg ctcgtgggag
     8761 cataaaggta gtaggctacc gaaccgccgg acgattcttg cagcacaaca caggacccat
     8821 aatcccccgc ggctcctgtc gtgccttggt aggttggaaa aatctgcgcc aacaccgact
     8881 gctggatgtc cgcagtcatt ttgcgcacgg cccgcgaccc ctggtcgcgc gcgatcattt
     8941 ccgtcgaatt gcttttgaaa agatgcagcc cttcgaccag gccgcctatg ctgatgaggc
     9001 caataatgct gccgatcgca ctggctatca gcacctctat cagcgtaaag ccgccggtga
     9061 agacgccccg aaaaggcgcc tgcgtttctc tgtaatcctc cgccgcgcgt cccatcacaa
     9121 tttgggaaaa tccccgcaga ctctcgtgct gatggcgtaa ctgagcggcg gccgggttga
     9181 gccggcatac agttgccact gaatcgtgat attgatctgc tgcagttggg ggatcactgc
     9241 gtccggcacg aattcaacct tccattggcc ttgcggaatt ttatccggga caaggttcgg
     9301 cgcggaagcc gacgaggaat tgacagcctc cacgggtaaa gactgccact ggccggcttg
     9361 cgggacgacc cacgccaaat ttggagaagc cgacccgaat cctttgagat aaatcgggac
     9421 ccccgccgtg ctgttgtgaa tatccgtata ttgcagcgat ttgaaaaggt cgatcatctc
     9481 cgcgccgatc gaggccacga gcaggcgctg ggaagccgtg cgattctcca tccgcatcca
     9541 cgaaatggct gccatcgttc caatcagcga aatcgttagg actgcgacag ctaccattac
     9601 ctcgaccagc gtcataccgg aagcggaacg atcgagggcc atccgtggtc gccccatctg
     9661 ttgggcaggg acgatcaaag ccgcacagtt ggaatccata cgatccataa ttactataac
     9721 gagcagattt agggccattc gacgccgcaa aaggccccca gaagacctgt agaatgcaac
     9781 actcgccagg gattcgatcc tccggaacga atcgtcagtc gcgatgcgac gtcatcgacc
     9841 catctacatc cgcccgcgcc gttgatagaa acggtagcgc tcgaggatgg attcgacgta
     9901 tttcctggtc ccaggaaaat cgatattacg ctggaaggcg ctcgccgaga tgggtcggct
     9961 gccgtcgccc ccggcccagc gttgcgcgcg gctcgcgccc gcgttgtatt ccgccaaggc
    10021 aaagggcgtc ggatcgcgcg cctgccgcca gtgcaacatc gtccggtgca ggtaccatgc
    10081 gcctacctcg agattcctct tcggatcaaa caactcttcc acccgaaaat tctccgtccg
    10141 attctctttc gaccagtcgc gggctgcgat ctcgctcacc tgcatcagcc cgcgctcccc
    10201 ggccgcgccc agcttgtgcg catcgaatcg gctctcccgc cagacgaccg ccttgaccag
    10261 catcggatcg aggccgtttg cggccgagac cacgtggatg agctgatcgt actgctgaaa
    10321 ccgcgccggg ctgatccatt cgtatagcgt gtaaaacgga tcgccggagc gcagggcgag
    10381 gagtccgatt ccagccccgg ccgccaggag gaagcagatg ataagtttaa aaaaaaagcg
    10441 gatcatcgat tcgttaggcg aaaaaccgcg actaccctgg atcgccgctg gataagttcg
    10501 ccgccgcgcg gaaagtcatg gtcagaatca attcacgcaa gatcggcctg agcaatttaa
    10561 tttcgcatgt ccgccttcgc ccgagtcatc atcgatcgct cgatccgccg cgagctcgat
    10621 tacagcgtgc cggaagagct ggccggtcga gttgttgtgg gctcgcgcgt tcgcgtgccg
    10681 tttcgcgata aatgggcgct cgccacggtc gttgacacgc tcgacgaaac ccccgccgaa
    10741 ggcgtgcggc cgatcgaggc cctcctcggg gatcgaccag tcctgagtgc gtgcctgatc
    10801 gagctggcgc gctggatgtc gacttactac tgctgccccg tcgagacggt gatgcggagc
    10861 ctgcttccgc aggtcatacg acgtgccgag gtcggctgga agaaacagct tttcgttcat
    10921 cccgcgcgga tcgccccgac ggaggagatc gaaaagctgc gcaagcgcgc gccgcgccag
    10981 gcggaattgc tggacgcgat cgcgcaactg cccgcgccga ttccggccgc ggatctcctg
    11041 cgccagactt cgctcgacaa ttccaccctc cgggcgctcg ttaggcgcgg cttcgtcgag
    11101 ctgcgcgaac aggcggtcgc gcgcgatccg cacggcacgg aagagttcat tgccgatacc
    11161 gagctggcga tgaacgagga acagactgcg gcgttaaaac tgatcgagac cgcgctgaaa
    11221 aatcccgaga cgcatccgcc gatcctgctc cacggtgtga ccggcagcgg caagacggag
    11281 gtgtacttgc aagcgattcg cgccacgctc gcgcgcggca ggaccgcgat cgtgctcgtc
    11341 ccggaaatct cgctcactcc gcagacggtc gagcgtttca aaagccgctt cgcagctcag
    11401 ccagacatgg tggccgtcct gcacagtcac ttgtcggaag gcgaacggca cgatgaatgg
    11461 cacaaactgc atgccgggcg ggcgcggatc gtgatcggtg cgcggagcgc ggtcttcgcg
    11521 ccgctcgaaa atctcggatt gatcgtggtc gatgaagaac acgagacctc ctacaaacag
    11581 gaggaggcac cgcgctatca cgcgcgcgac gttgcggtcg tgcgggcgaa aatcgagaaa
    11641 tgcgtcaccg tcctcgggac ggcgaccccg tcgctcgaaa gttacgccaa cgcgacccgc
    11701 gggaaatatg tcctcagccc gctcacccag cgggtcgatc aatgccagat gccattgatt
    11761 cggatcgtcg atctgcggca ggagcgccga aaggaaaaag cggcggcgat tctctcagag
    11821 aaattgcgta gcgcgatcgg caatcgactc gaaaaacacg agcaaaccat cctgtttctc
    11881 aaccgccgtg gtttctccac ctcgctcctt tgcagccact gcggcaaagc gcgcgactgc
    11941 ccgaactgca gcgtcgccct cactttccat cggagtaacg cccgcctgag ttgccacctg
    12001 tgcgggcata gcgcggccgt gccgcagcgt tgtccggagt gcggccagga cgcgctcatc
    12061 tatgccggtt tcgggacgga aaaagtggaa gcaaacgtct ccgcgatttt tcccaaagca
    12121 gtcgtcaggc gaatggacgc cgactcgatg acgcgtaaag acgcctaccg cgaaacgctc
    12181 cacgcctttc gttccggcaa gatcgatatt ctcgtcggca cgcagatgat cgcgaaaggt
    12241 ctgcacttcc cgaacgtcac acttgtcggg atcatcaacg ccgatctcgc gctgcatttg
    12301 ccggatttcc gcgcggggga acggacgttt cagctcctca cccaggtggc cggccgcgcg
    12361 ggccgcggcg aagcgccggg cgaagttttc gtgcaaactt acacgccgtt cagcccgtcg
    12421 atccaattcg cgcgacatca cgatttcgcc ggctactttg agcaggaact cgagttccgg
    12481 gagcgctgcg atttcccgcc cttcaaacac gccgtcctta tcaccgtgcg ctccgagcat
    12541 gaaacccacg ccagtttttc cgcggagacg ctggcgcggc ggttaagaga agcgttgccc
    12601 gaggaattca cgctcggcgc tcccgctccc gcaccgctcg aaaagttaca gggacactat
    12661 cgctttcaca tcctgctgcg cggcgaagcg atcttgcgtt tgacccgttt gattcgcgaa
    12721 acgctcgaca agctgccctt tcccgaggac gtcgcggtcg cggtggacgt cgatccgtat
    12781 caactcttgt agacgccgcc ctctggccgg cgccggggga atggtgccga cccctgccgc
    12841 cccgccgaag ctcgcgcttc gcagagggaa acgcctacat gcgctactcg actacgacgc
    12901 ccttccagaa ggcgacatga tc
//
LOCUS       DATMNS010000105         4266 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4377389, whole genome shotgun sequence.
ACCESSION   DATMNS010000105 DATMNS010000000
VERSION     DATMNS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4266
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4377389"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            355..849
                     /locus_tag="VNW28_03795"
     CDS             355..849
                     /locus_tag="VNW28_03795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA09078.1"
                     /translation="MRRQPTPFHPDPEKIATLEIDVSPELVPLEIEVPPKVPAEPARR
                     ALLLEDGDDFREVLRDHLVLRGFQVTSVSSGVEGILEIRKDPFDIIVCDMMMPRMGGE
                     MFYWAVTRMRPATAQHFIFFTGHRNNPAIEFFFKRVNAPVLVKPFELSALDSTIDDVL
                     RKLV"
     gene            complement(1061..3694)
                     /locus_tag="VNW28_03800"
     CDS             complement(1061..3694)
                     /locus_tag="VNW28_03800"
                     /inference="COORDINATES: protein motif:HMM:NF042912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Amuc_1098 family type IV pilus outer membrane
                     protein"
                     /protein_id="HXA09079.1"
                     /translation="MNDLSRRLLTFVFALLVTLQGGRVRAENVRRIAEHEIARRQAGV
                     LQGQGAAARGKLALQANDFAKAHDEFRVAVNFLPDAITTADQHDAAVKGFCESGVKLA
                     EQRIAEGKYGEAEQLVREVVSDRYDPNCRDALNLLAHFSEPGHFNKTIGPKFIVKVED
                     VKKLLSDADGYYDSGRYDLAFKKYEQVLNLDPYNVAARRGEERIEHTKTHYGERAYNE
                     TRARELWRVQKGWEEPVRQYGQTVGPISDAFARDAGGTARITNKLNSIIIPKIEFRDA
                     SVREAIDFLRQQAAANDLSTEGKKGVDIVLRTNALGRAEAPAPLSETAPPLPGVAPEA
                     GGIEPTPVPAPVASSISPGAARITITLNQIPLGEALRYIASQAGLKAKVEPYAVSVIP
                     INEQSSDLFTKEYRVPPGFISSSVNIGASALDKGPTRVGAGGTGKDTQESTGGQQLVN
                     RQTALDFLKDQGVPFPTGASANFLPQSSRLIVRNTEENLELVDALVEQANVAGPKQVE
                     IEAKFVEITQNNLKELGFDWLLGSFDAAENGKLVASGGTSGTSPAVVPGDYPILQNGV
                     PVGQNPVTAGNRSGNLALSANAIDALLFGVPGASSLAPGIFGLSGVMTSPQFQVVVRA
                     LNQKKGVDLLSAPRVTTKSGQRAVIEIVREFRYPTQFDPPKIPDTVGSTGNNSGGGTA
                     SIPVTPTTPTGFETRNTGVTLEVEPVVGPDGVTIDLNLVPQVVEFEGFINYGSPIFSA
                     ATSGSVTLMGMNVITIPGVGQSVLTPNVINQPIFNSRKVTTSVSVWDGQTVVLGGLMR
                     EDVQKTEDRTPIIGDIPVLGRLFRTNTEQHIKRNLVIFVTARLVNPAGQPVNSSEEEE
                     ETETVSQLEPPVLPQLPYYKK"
     gene            complement(3950..>4266)
                     /locus_tag="VNW28_03805"
     CDS             complement(3950..>4266)
                     /locus_tag="VNW28_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09080.1"
                     /translation="YDTNKYGTMIDVSELTLEQVDSRDRVTLVKERRTTSPESVANFL
                     YTWGGAEQSFAVRKDQEFSLKPLEQIKYKLLDVQPDKAIIIDTQKPNEKIEIGPAPRP
                     NP"
BASE COUNT          811 a         1247 c         1262 g          946 t
ORIGIN      
        1 caatgggggg caacgcaaag ggggaaaggg agtagggggt cgagtagggg tcgggtcgag
       61 taggggtcga gtaggggtca ggcatcaaat cttgacaacc gacggtttcc gcaagggaag
      121 gcatgccgcg cagcatccgc atcgagtatc ccggggtctt ttactacgtc atggcccgag
      181 gcaatcggcg catgaagaga aatgggggtc gcatcagacg cgacgtgacc gggaattggc
      241 gctaagcggt tacctcgatc gccgccaatc cacaaacggt gatgtgatcc ttttcaatcg
      301 gggaccgatg tcggagggcc cgagggcatc tgttctgctc attgactttc gtttatgcgt
      361 cgacaaccga cacctttcca tcccgatccc gaaaagatcg ccactctgga gattgacgtt
      421 tcccccgaac tcgttcctct cgagatcgag gttcccccga aagttccggc cgagccggcg
      481 cggagggctt tgctcctgga agatggcgat gatttccggg aagttctccg cgatcacctg
      541 gtcttgcgcg gttttcaggt aacgtcggtc tcgagtggcg tcgagggcat ccttgaaatc
      601 aggaaagacc ccttcgatat catcgtttgc gacatgatga tgccgcggat ggggggcgag
      661 atgttttact gggcggtcac gcggatgcgg ccggctaccg cccagcattt catttttttt
      721 accggccacc ggaacaatcc ggcaatcgag tttttcttca agcgagtgaa cgcgccagtg
      781 ctcgtcaaac cgttcgagtt gtcggcgctc gattcgacga tcgacgatgt tttgcgcaag
      841 ctagtctgat acgcccgcct tcgcgcttcg gcgtcgacga aaaggctcgc tacttacctt
      901 ggctttatta ctgctcggtc catggccaac ttcggcggaa gcgccacggc ccgagtcaaa
      961 gtaaagtaag acttctgtga ggcaaacctt gggttagcca agacgattac aaatagtgga
     1021 cttgccggta gagatgccac tgatgatccc gataaccacg ttattttttg taatagggga
     1081 gttgcggcaa gaccggcggt tcgagttggc tgaccgtctc ggtctcttct tcttcttcgc
     1141 tcgaattaac cggttgaccg gccggattga ccagacgcgc ggtcacgaag ataacgaggt
     1201 tgcgcttgat gtgctgctcg gtgttggtcc ggaaaagccg gccgagcacg gggatgtcgc
     1261 cgatgatcgg agtgcggtcc tcggttttct gcacgtcctc gcgcatcaag ccgccgagga
     1321 cgacggtctg gccgtcccac acgctcacgc tcgtcgtcac tttgcgcgag ttgaagatcg
     1381 gctggttgat cacgttgggt gtgaggacgc tttgccccac ccccgggatg gttatgacat
     1441 tcatacccat cagcgtaacg ctgccgctgg tggcggccga aaagatgggg cttccgtaat
     1501 tgatgaagcc ttcgaattcg acgacttgag gcacgagatt cagatcgatt gtgactccat
     1561 cagggccgac cacgggctcg acttcgagcg tgacacctgt attccgggtc tcaaagccgg
     1621 tgggcgttgt gggcgttacc ggaatcgacg cggtacctcc tccggaattg ttacccgtac
     1681 tgcccacggt atcaggaatc ttcggcggat cgaactgcgt cggatagcga aactcccgca
     1741 cgatctcgat cacggcgcgc tggccacttt tggtcgtgac ccgcggcgcg gagagaagat
     1801 cgacgccttt cttctggttc agcgccctaa cgacgacctg gaattggggg ctggtcatga
     1861 caccgctcaa tccgaagatg cccggggcga gcgagctggc tcccggaacg ccaaagagga
     1921 gggcatcaat ggcattcgcg ctgagggcga gattcccgct acgattgccg gcggtgaccg
     1981 gattctgacc gactggaact ccgttttgta gaatcggata atcgcccggc acgaccgcgg
     2041 gagaggttcc ggaggtaccg ccgctggcta ccagtttgcc gttttcggcc gcatcaaatg
     2101 agcccagcaa ccagtcaaaa ccaagttcct tcaagttgtt ttgcgtgatc tcgacgaact
     2161 tggcttcgat ctccacttgc tttggacctg ccacgttggc ctgttccacc agcgcatcca
     2221 ccagttcgag attttcctcc gtgttccgca caatcaaccg gctgctttgc gggaggaaat
     2281 tcgcactcgc cccggtcgga aacgggaccc cctggtcctt caggaaatcg agcgcggtct
     2341 gccggttcac gagttgctgg ccgccggtcg actcctgagt gtctttgccg gtgccgcccg
     2401 cacccactcg cgtgggcccc ttgtcgagcg cgcttgcccc gatgttgacc gaactgctaa
     2461 tgaaacccgg cggcacccga tattccttcg taaacaaatc gctgctctgc tcgttgattg
     2521 gaataaccga gaccgcatag ggctccacct tcgccttcaa tcccgcctgg ctcgcgatgt
     2581 agcggagcgc ttcgcctaac ggaatctgat ttagcgtgat cgtgatccgc gcggcgccgg
     2641 gcgagatcga cgacgcgacc ggcgccggca ccggcgtcgg ttctatgccg cccgcctcgg
     2701 gcgcgacgcc cggcaagggc ggtgcggtct cggacagcgg cgcgggcgcc tcggcccggc
     2761 cgagagcatt ggtccgcaga acaatgtcga cacctttctt tccctccgtg gagagatcat
     2821 tcgcggccgc ttgttgccgc agaaaatcga tcgcctcccg gacactcgcg tcgcgaaact
     2881 cgattttggg aatgataatg ctgttcagtt tgttcgtgat ccgcgccgtg ccccccgcgt
     2941 cgcgggcgaa ggcgtcgctg atcggcccga cggtctggcc gtattggcgg accggttcct
     3001 cccagccttt ttgcacccgc cagagctcac gcgcacgagt ttcgttgtag gcccgctcgc
     3061 cgtaatgcgt cttcgtatgc tcgatccgct cttcgccgcg gcgggccgcg acattatagg
     3121 gatcgaggtt cagcacctgc tcgtatttct tgaaagcgag atcgtagcgg ccggaatcgt
     3181 aataaccatc cgcgtcgctc aagagcttct tcacgtcctc gaccttgacg atgaacttcg
     3241 ggccgatcgt cttgttgaaa tgcccgggct cggaaaaatg ggcgaggagg ttgagcgcat
     3301 cccggcagtt cgggtcgtag cgatcgctga ccacttcacg caccaactgc tcggcctcgc
     3361 cgtacttgcc ttcggcgata cgctgctcgg ccagtttcac cccgctctcg caaaatccct
     3421 tgaccgccgc atcgtgctgg tcggcggtcg tgatcgcatc gggcaaaaaa ttcacggcca
     3481 cccggaattc gtcatgcgcc ttggcaaaat cgttcgcttg cagcgcgagc ttgccccggg
     3541 ccgccgcgcc ctgcccctgt aaaactcccg cctgccgccg ggcgatctcg tgttccgcaa
     3601 tacgccgcac attctccgcg cggacgcgcc cgccttggag cgtaacaaga agggcgaaaa
     3661 cgaaagtcag gaggcgccga gatagatcat tcatggggta gttcatgggg tagttcatgg
     3721 ggtaggtgtg gtggttgtat tcggagccgg tgactccagc aaggaaataa cgccgggaaa
     3781 aatgacgaat tccgaatgac caaggtcgaa ggaattccaa atgacaacgc ccgaaggtgg
     3841 gagcgtgctg gccaccgcat tcgtcattag tgcttcgtca ttccttcgtc atttgtcatt
     3901 ttcctccgcg ccacagaaaa cctgacctgc ttgcggcaaa tttcagaatt caaggattcg
     3961 ggcgcggcgc cgggccgatc tcaatcttct cgttaggctt ttgcgtgtcg atgatgatgg
     4021 ctttgtcggg ctgaacgtcc agcagtttgt atttgatctg ctccaacggt tttaaggaaa
     4081 attcctgatc ttttctgacg gcgaaggact gttccgctcc gccccaggtg taaagaaagt
     4141 ttgcgaccga ctcgggcgag gtggtgcgcc tctctttcac cagcgtcacg cgatcgcgcg
     4201 aatccacctg ttcaagagtc aattccgaga cgtcgatcat cgtcccatac ttgttggtat
     4261 cgtatt
//
LOCUS       DATMNS010000106         7828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1869216, whole genome shotgun sequence.
ACCESSION   DATMNS010000106 DATMNS010000000
VERSION     DATMNS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7828
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1869216"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..2595)
                     /locus_tag="VNW28_03810"
     CDS             complement(7..2595)
                     /locus_tag="VNW28_03810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09081.1"
                     /translation="MIQDLRYALRLLFKSPGFSFIAILTLGLGIGVNTAVFSLIHTLF
                     FQPPAYARPAEIVQLFSQDTKNPKTFRGFSYPTYRDIREQNTVFSGLCAHNLALVGLG
                     ERGDTRRAFADVVSSNYFSVLGHSPAQGRSFLPEEETPGRNIPVAIVSHNYWKKQGFD
                     PALLGSKMVINGRPYTIVGIMPEGFTGTMQIISAEVWLPLGVYGQVANDFEQDNRNSL
                     GDRFGTQLLLIGRLKPGLSAAAALPALKTLATNLERAYPVEQKNQTFMTAPLSRFSTS
                     TSPQGDGFLSGVAPLLLGMSLIVLFVACLNLANMLLARGTARRKEIAIRQALGGSRGR
                     IVRQLLLEGFVLALCGGGLGILLGLWSSDLLFASLGHLLPLDLVWQSGPNPALLGATF
                     VFCLIGTICFALGPALKLSRGLIIGDLKENAAEDVVRRHWKFLPRNPLVVAQLAFSLA
                     LLTAAALFIRGANQAAGLDPGLKVEHSFLLEVDASLGGHNQKQSREIYRQLQERLAAL
                     PGVEHASISATVPFGMISLSKKVQRAGINISPDAHPATAADGLAFDARWDSVGADYFT
                     TVGLRFLRGRAFHVAEATNPGGPAVAIIDDVLAKKLWPGGDALGQQIQIAVDAAPSKG
                     GGRAGIGMNDDSSGALKPNEPIEIIGIVPATRDALFEKELPGTLYLPFARGFQSNIFF
                     IIKFATLDRASETAAADLIRRTVREVDPVLPILSLKTFSQHLESDVELWVVRAGAALF
                     SVFGGLALTLAVIGIYGVKAYAVARRRREIGIRMALGARPGVVQWMFMRETAVMLVSG
                     VALGLVFAVLTGKVVSGLLYEVGALDPVAFTLAPLVLGSAALLATWLPARRAARVDPM
                     VALRAE"
     gene            complement(2812..5253)
                     /locus_tag="VNW28_03815"
     CDS             complement(2812..5253)
                     /locus_tag="VNW28_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09082.1"
                     /translation="MLIDLRYAFRQLIKSPGFTFIAVLTLALGIGANSAIFSVIDTVL
                     LRSLPFPNPDRLTMVWASAPQHPGDDRQVHSYPDYLDLRAQNHTFSALAAYTGASTIW
                     GIGENSEDVPGIAATSDLFAVLGTPPLLGRGFSQADDKPDAARVIVIGYSFWQRHFAG
                     DPKIIGKQVTVAGKVYTITGVMPRGWKFPMQNENVDYVAPLLPMFSGSTPNSIMRRGA
                     HFLPVVGRLKPGVDLRTATADLQTIAAQLAKQYPDTDAGRTERAVPLQSDLVGDVRPA
                     LLVLVAAVALVLLIACANVANLFLARAATREREIAIRTALGANRFQIVRQLLLETSLL
                     ALLGGAAGLLLAWWSIDALVAMGPTDLPRLDEIRVDGVVIAFTFGTAFLTSLIFGLIP
                     ALQASRPQVDQSLREASRGSTGGVRSHRLRFAFVVSQFALSLVLLVGAGLLIRSFAEL
                     RAVEPGFEARGVVTFWQALPKAHYGEVDQQIQFFDRLLAKLTSLPGIENAGMVSPLPF
                     SGNDQGRTFTIVGQPAPAPGMEPSASLLTTDGAYFRTMRIPLKKGRTFDPRERKDSTP
                     VVMINETFAQKYFPNENPIGQRLDIGGRVAEGKPACEIVGVVGTAKHGSLAEADAPEF
                     YLPFAQNPDRFMDLVVRTSQPAPTGLETMLRRAVHELDAQQFVPVITPLSQLVSQTLA
                     QSRFNTGLLAVFAAVAIILAAVGIYGVIAYTVAQRTREIGIRMALGAQRQQMLTMILR
                     QSLTMAAIGIGVGLLGAFAATRLLRALLFGVGTTDLITYGTVIFLLGAAALLAGLLPA
                     RRAMKVDPVIALRYE"
     gene            complement(5268..7670)
                     /locus_tag="VNW28_03820"
     CDS             complement(5268..7670)
                     /locus_tag="VNW28_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09083.1"
                     /translation="MNDLRYAFRQLIKSPGFTFIAVLTLALGIGANSAIFSVIDTVLL
                     LPLPYPHSEQIVTVSQTVRSTGVSTEDVSPANFIDWQTQNSVFSAMACAHGWPANLSG
                     GEQPERVRATVVSAQFFSLFGAQPILGRTLGPNDAKPGNSHVVVLGHELWVRRFGGDR
                     SILGRDLLLDGEKFTVVGIVPAGFSPDEYGELWVPSPWDVPVHPLSPNDNPREMRDRS
                     YLDAWARLKTGVTLARAQAEMSAIAARLEHQYPDADQDTGIALVPLHEQMVGGIRPML
                     LMLLGAVSLVLLIACANVANLLLARAAARSREIAIRTALGASRLRLVRQLLTESSLLA
                     ALGGALGVLLAVWALPVLLSFSPPEMGDFNHVGLNGQVLIFSLITSVATGALFGLAPA
                     FFASRSNPNESLREGERGSSLGKSPARSVLIAAEIALSLVLLVGAGLMMKSFVRLTQV
                     NPGFNPDHLLVFNIGLPSSAEPARQISFYEEVVERLRALPGVESVGAVSRLPLAGGNS
                     SRSFNIPGSGQSYDADIRVSTPEYFKTMGISLLKGRNFAAHDAGDSLPVAIINETAAA
                     NVFPGQDPIGKYLTNFGPKNEKLQIVGVVGNVHHVGLETAARPEVYLPLAQSQWPSMY
                     VAVRSAVSNPLTLIPSVQEAVWSIDRNVPLANPRTMQDVLAHSVLRRRFAMLLLSIFA
                     GLATLLAAIGLYGVISYSVAQRTKEIGIRMALGGQRSEMLRMVLRQSGKLVSIGMLVG
                     VPVAFGATHLLGALLYGIGATDITTYLFVIALLGAAAFFASIIPAFRATKVDPMVALR
                     YE"
BASE COUNT         1589 a         2319 c         2595 g         1325 t
ORIGIN      
        1 agcggttcat tcagcgcgca acgcgaccat cgggtcgacc cgcgcagccc ggcgcgcggg
       61 cagccacgtg gcgagcaagg ccgcgctccc gaggacgagc ggcgcgagcg tgaaggcgac
      121 cggatcgagc gcaccgactt cgtagagtaa cccgctcacg acttttcccg tcagaacggc
      181 gaagacgaga cccagcgcga cgccgcttac gagcatcacc gcggtctcgc gcatgaacat
      241 ccattgcacc actccgggcc gcgcccccag cgccatgcgg atgccgattt cccttcggcg
      301 ccgcgccacc gcgtaggctt tgacgccgta aatcccgatc acggcgagcg tgagcgcgag
      361 tccgccgaag acggagaaga gcgccgcgcc ggcgcgcacg acccagagtt ccacgtcgct
      421 ctcgaggtgt tgcgagaacg tcttgagcga gaggatgggc agcaccggat cgacttcgcg
      481 cacggtgcgg cggatcaggt cggccgcggc cgtttcgctg gcgcggtcga gggtggcgaa
      541 tttgatgatg aagaaaatgt tgctctgaaa accgcgggcg aacggcagat agagggtgcc
      601 cggtagttcc ttctcgaaaa gtgcatcgcg cgttgccggc acgatgccga tgatttcgat
      661 cggctcgtta ggcttgaggg cgccgcttga gtcgtcattc atgccgatcc cggcccggcc
      721 gccgcctttc gaaggcgcgg cgtcgaccgc gatctggatc tgttgcccga gcgcgtcgcc
      781 cccgggccag agttttttcg ccaggacgtc gtcgatgatc gcgaccgcgg gtccgccggg
      841 attggtcgcc tccgcgacgt ggaacgcccg gccccgcaga aaacgcagtc cgaccgtggt
      901 gaaataatct gcgcccacgc tgtcccaacg ggcgtcgaaa gcgagaccat ccgcggccgt
      961 cgccggatgc gcgtccggcg atatgttgat cccggcgcgc tgcactttct tgctcagcga
     1021 aatcatcccg aaaggcacgg tggcggagat gctggcgtgc tcgacaccgg gcaaggcggc
     1081 gagccgctcc tgtaattgcc gataaatctc gcgggactgt ttttggttgt ggccgccgag
     1141 gctggcgtcg acctcgagga ggaagctgtg ctcgaccttc aacccgggat caaggccggc
     1201 ggcctggttc gccccgcgga tgaaaagcgc cgcggcggtg agaagggcga gggagaaagc
     1261 gagctgcgcg acaacgagcg gattgcgcgg gagaaatttc caatgtcgcc taacgacatc
     1321 ttcggcggcg ttttccttca aatcgccgat gatgaggccg cgcgagagtt tgagggccgg
     1381 cccgagcgcg aagcagatcg tgccgatcag gcaaaaaacg aaagtcgcgc ctaacaatgc
     1441 cgggttgggg ccgctctgcc agacgagatc gagcgggagc aggtggccga gggaagcgaa
     1501 gagcagatcg gaggaccaga gcccgaggag gatgccgagc ccgccgccgc agagagcgag
     1561 gacgaaaccc tcgagcagga gctgcctaac gatccggccg cggctcccgc cgagcgcctg
     1621 gcggatggcg atttccttcc gccgcgccgt gccgcgggcg aggagcatgt tcgcgagatt
     1681 gaggcaggca acgaaaagga caatgagcga catcccgagg agcagtggag cgacgccgct
     1741 caggaagccg tcgccctgag ggctggtgct ggtggagaaa cgcgagagcg gcgcggtcat
     1801 gaaggtttgg ttcttttgct cgaccggata ggccctttcc aaattcgtcg cgagcgtctt
     1861 gagcgcggga agcgcggcgg ccgcgctcag gcccggtttg aggcgaccga tgagcagcaa
     1921 ctgcgtgccg aagcggtcgc ccaacgagtt gcggttatcc tgctcgaaat cgttcgccac
     1981 ctggccgtaa acgccgagcg gcagccagac ttccgcggag atgatctgca tcgttccggt
     2041 gaatccttcc ggcatgatcc cgacgatcgt atagggccgg ccgttgatca ccattttcga
     2101 accgaggagc gcgggatcga atccctgttt cttccaatag ttgtgactca caattgcgac
     2161 cggaatgttg cgcccgggcg tttcttcctc cgggagaaag gagcgtccct gcgccggaga
     2221 atgaccgagg acggagaaat aattcgagct cacgacatcg gcaaaggcgc gccgcgtgtc
     2281 gcctctttct cccagcccga cgagcgcgag attgtgcgcg cagaggccgg agaagaccgt
     2341 gttttgctct cgaatatcgc gatacgtcgg ataggagaaa ccgcgaaatg ttttcggatt
     2401 cttcgtgtcc tgggaaaaga gctgcacgat ttcggccggc cgcgcgtagg cgggcggctg
     2461 aaaaaagagc gtgtgaatga ggctgaagac ggcggtgttg acgccgatcc cgaggccgag
     2521 ggtgaggatg gcgatgaagc tgaagcccgg cgacttgaag agaagccgga gcgcgtagcg
     2581 aagatcctgg atcatggtgt ttgtttagtt gttgaggtaa gttttttggc ctaacgatta
     2641 ttctgtgcgc agggcttcga tcgggtttac tttggtcgcg ccttgcgccg ggaggagaca
     2701 ggagacgagg gcgatgctgt ggtaattcag gcctctggcc tgacaggtcg cgcgggcgtc
     2761 tcgcccgcag tttggctgca tcacgcaggc gagacggccg ggaccgctgt gttactcata
     2821 gcgaagcgcg atcacaggat cgactttcat ggcgcgccgt gccggtagaa gtccggccag
     2881 gagtgcggcg gccccgagca ggaaaatgac cgtgccgtag gtgatgaggt ccgtcgtccc
     2941 aaccccaaag agcagcgcgc ggagcaagcg cgtggcggcg aacgcgccga gcaatcccac
     3001 tccgattccg atcgccgcca tcgtcaggct ttggcgcagg atcatcgtca gcatttgctg
     3061 gcgttgcgcg ccgagcgcca tccggatccc gatttcacgc gtccgctgcg cgacggtgta
     3121 ggcaatcacg ccataaatgc cgacggcggc gagaatgatc gcaaccgcgg cgaagaccgc
     3181 gagcagaccg gtgttaaagc gtgattgcgc gagagtctgg ctaacgagtt gcgagagcgg
     3241 ggtgatcacg ggcacgaatt gttgggcatc caactcgtgc acggcgcgcc gaagcatcgt
     3301 ctcgagcccg gtcggcgccg gttgactcgt ccgtaccacc aggtccatga acctatccgg
     3361 attctgcgca aaaggaagat agaactccgg agcgtcggct tccgcgaggc tgccatgctt
     3421 cgccgtcccg acgacgccca cgatctcgca ggccggcttc ccctccgcca cgcgaccccc
     3481 gatatcgagg cgttgtccga tcgggttttc gttagggaaa tatttctgcg cgaaggtttc
     3541 gttgatcatc acaaccggcg tcgaatcctt gcgctcgcgc gggtcgaagg tgcggccctt
     3601 cttcaacgga atccgcatcg tccggaaata agcaccgtcg gtcgtgagga gcgaagcgga
     3661 cggttccatt cccggcgcgg gagcgggctg gccgacaatc gtaaaggtgc gcccctggtc
     3721 gtttccgctg aagggcagcg gcgaaaccat gcccgcgttt tcaattcccg gcagcgaagt
     3781 caatttcgcg aggagtctgt cgaaaaactg tatttgctga tcgacctcgc cgtagtgcgc
     3841 cttggggagc gcctgccaga atgtaacaac cccacgcgcc tcgaagcctg gctcgactgc
     3901 gcgcagttcg gcgaagctgc ggatgagcaa gcccgccccc acgagcagga ctaacgaaag
     3961 ggcaaactgc gagacaacaa aggcgaagcg caaccgatgg ctgcgcactc cgccggttga
     4021 accacgggaa gcctcccgca gcgattggtc gacctgcggg cgggaagcct gcagagcagg
     4081 tatcaatccg aagatcaggc tggtcagaaa tgcggtgccg aaggtgaagg cgatcacgac
     4141 gccgtccact cgaatttcat ccagtcgcgg aagatcggtc ggtcccatcg cgacaagcgc
     4201 atcaatgctc caccaggcga gcaacaatcc cgccgcgccg ccgagcagag cgagcaagga
     4261 agtctcgagc aggagttggc gcacgatctg aaaacgattc gcccccaggg cggtgcggat
     4321 tgcgatttcg cgctcgcgcg tcgcagcgcg ggcgagaaac aggttcgcga cgttggcgca
     4381 ggcgatcagg aggacgagcg cgaccgccgc gacgagcacg agcaacgccg gtcgcacatc
     4441 gcccaccaaa tccgattgca ggggcaccgc gcgttcggtg cgtccagcgt cggtatcagg
     4501 gtattgcttc gcgagttggg cggcgatcgt ctgcagatcc gcggtcgccg tgcgcaagtc
     4561 gacgcccggt ttcaggcgcc cgacgacagg aagaaaatgc gctccccgtc gcatgatgga
     4621 gttcggagtc gagccggaga acatcggcag aagcggcgcc acgtaatcga cgttttcgtt
     4681 ctgcatggga aacttccaac cgcgcggcat cacgccggta attgtgtaaa ccttgcccgc
     4741 caccgtgacc tgtttgccga tgatcttcgg atcaccggcg aagtgcctct gccagaagga
     4801 atatccaatc actatcaccc gcgccgcgtc gggcttgtcg tcggcctgcg agaaaccgcg
     4861 gccgagcagc ggcggggtgc cgaggaccgc aaagagatcg gaagtggccg cgatccccgg
     4921 gacgtcttcc gaattttccc cgatacccca aatcgtggaa gcgccggtgt aggccgccaa
     4981 cgccgagaag gtgtggtttt gcgcccgcag atcgaggtag tcggggtagg aatgcacctg
     5041 gcgatcgtcg cccggatgct gcggcgcgct cgcccagacc atcgttaggc gatccgggtt
     5101 tggaaaaggc agggagcgca gcaggaccgt gtcgatcacg ctgaagatgg cgctgttcgc
     5161 cccgatcccg agcgcgaggg taagcacggc gatgaaggtg aaaccggggg atttgatgag
     5221 ttgccggaag gcgtagcgga gatcgattag catatcgtta ggcaagttta ctcatagcga
     5281 agcgcgacca tgggatcgac tttggtggcc cgaaatgccg ggatgatgct ggcgaaaaat
     5341 gcggccgcgc cgaggagcgc gatcacgaaa agataggtcg taatgtcggt cgccccgatg
     5401 ccataaagca acgctcctag caagtgagtc gcgccaaatg cgaccggcac accaaccagc
     5461 attccgatcg aaacgagctt tccgctttgt cgcaggacca ttcgtagcat ctcgctgcgc
     5521 tgcccgccga gggccatccg aattccaatc tctttcgttc gctgggcgac cgaatacgaa
     5581 atcacaccgt aaagcccgat cgccgcgagc agggtcgcga gcccggcgaa aatcgagagg
     5641 agcagcatgg cgaatcttcg ccgcagaacg gaatgcgcca ggacgtcctg catcgtgcgc
     5701 ggattcgcca gaggaacgtt gcgatcgatg ctccaaactg cctcctgtac cgatggaatg
     5761 agggtcaacg gattcgagac cgcgctgcgc accgcgacat acattgaagg ccactggctc
     5821 tgggcgagtg ggaggtagac ttccggtcgc gcggccgttt ccaatccaac gtggtgtaca
     5881 ttcccgacca cgccgacgat ctgcagcttc tcattcttcg gtccgaaatt tgtcaggtat
     5941 ttgccgatcg gatcctgacc gggaaacacg ttcgcagccg ccgtttcgtt gataatcgcg
     6001 accggaaggg agtcgccggc gtcgtgggcg gcgaagttgc gcccttttag gagggaaatc
     6061 cccatcgttt tgaaatattc cggcgtgctc acccggatat ctgcgtcgta ggactggccg
     6121 ctccccggga tgttgaagct gcgcgagcta tttccgccgg cgagcggcag gcggctgacc
     6181 gccccgaccg actccactcc cggcagtgcg cgcaggcgct cgaccacttc ctcgtaaaac
     6241 gaaatctggc gcgcgggctc tgcggaagag gggaggccga tgttaaagac cagcagatga
     6301 tccggattga agcccggatt gacctgggtc agccggacaa aacttttcat catcaggccc
     6361 gcaccgacga gcaggacgag cgagagcgct atctcggcgg cgatcagcac cgagcgggcc
     6421 ggactcttgc ctaacgaact tccgcgttcg ccctcgcgaa gcgattcatt tggattggag
     6481 cgcgaagcaa aaaaggccgg cgccagacca aatagggcgc ctgttgcgac ggaggtgatg
     6541 aggctgaaaa tcagcacctg accattcagg ccgacgtggt tgaagtctcc catctcaggc
     6601 gggctgaatg aaagcagaac cgggagcgcc caaacggcca gaagcacgcc gagcgctcca
     6661 ccgagcgcgg ccaggagcga gctttctgtc aggagttgcc taacgagtct gagacgcgaa
     6721 gcgccgagcg cggtgcggat cgcgatctcg cgcgaacggg cggcggcgcg ggcgaggaga
     6781 agattcgcca cgttggcgca ggcgatgagg agaacgaggg aaaccgcgcc taacaacatc
     6841 aggagcatcg gccgaattcc cccgaccatt tgctcatgca acggaacgag cgcgatgccc
     6901 gtgtcctgat cggcatccgg atactggtgt tcgagacgcg ccgcgatcgc gctcatctcc
     6961 gcctgggccc gggcgagcgt gaccccggtt ttcaaacgcg cccaggcatc gagataactg
     7021 cggtcgcgca tctcgcgcgg attatcgtta ggcgagaggg gatgaaccgg gacgtcccaa
     7081 ggcgacggga cccaaagttc gccatactcg tccggcgaaa aaccggccgg cacgattccg
     7141 acgacggtga acttttcgcc gtcgagaagc aggtcgcggc cgaggatgct tcgatcgccg
     7201 ccgaagcggc gcacccaaag ctcatgaccg agcacgacca catgtgagtt acccggtttg
     7261 gcatcgttag ggccgagcgt ccgaccaaga atcggctgcg cgccgaagag cgaaaagaat
     7321 tgcgcggaga ccaccgtcgc ccggacgcgt tccggttgtt cgccgccgga aaggttggcc
     7381 ggccagccgt gggcgcaggc catggccgag aagacggaat tctgcgtttg ccagtcgatg
     7441 aagttcgctg gtgaaacgtc ttcggtcgag acgccggtgc tgcggacggt ctgcgaaacc
     7501 gtcacgatct gctccgagtg cggatacgga agcggtagca acaacaccgt gtcgatcacg
     7561 ctgaagatgg cgctgttcgc cccgatcccg agcgcgaggg tgagcacggc gatgaaggtg
     7621 aaaccggggg atttgatgag ttgccggaag gcgtagcgga gatcgttcat tggatttccg
     7681 attggttatt tgaaaggctg gcctcgcctg ctccgcagac tgcgtgaagg ctgattggtg
     7741 gtttcaagat cattcggcgc gtaaggcaat catcgggtca acccgcgagg cacgacgcgc
     7801 ggggatgagg caggccacga gtgacacc
//
LOCUS       DATMNS010000107         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4377508, whole genome shotgun sequence.
ACCESSION   DATMNS010000107 DATMNS010000000
VERSION     DATMNS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4377508"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..575
                     /locus_tag="VNW28_03825"
     CDS             147..575
                     /locus_tag="VNW28_03825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXA09084.1"
                     /translation="MQTKIAEALTTAEILRCFPVMRQLRTHFEDGERFVAQVERQRGE
                     GYHLAFLEDEKGVRAVAGYRLAESLHAGKFCYVDDLVTDAAGRSLGYGGALFAWLVAE
                     ARAAGCGKLELDSGVQRFAAHRFYLTKRMVISSHHFSLEL"
     gene            complement(681..1613)
                     /gene="deoC"
                     /locus_tag="VNW28_03830"
     CDS             complement(681..1613)
                     /gene="deoC"
                     /locus_tag="VNW28_03830"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HXA09085.1"
                     /translation="MKALPASNNRHKALRARWEIPSVDQVMVEERAAAFGKRSIKSSA
                     KLTGLKLAVSMMDLTTLEGKDTPGKVAFLCRKAMQPVDPLYDVPSCAAVCVYPNLVRV
                     AKRLLGASRVKVASVATAFPTGLMPLDLKLEEVRRASGDGADEIDMVIDRGVFLAGDY
                     NRVFDEVAATKEACGEAHLKVILETGELQTYDNVRLASEIGIRAGGDFIKTSTGKVTP
                     GATIPVTLVMLEAIRDYFYETGVRIGMKPAGGVRNAKQALAYLVMVKETLGDDWLTPD
                     LFRFGASTLVNDILMQIVKTVDGHYQSADYFALP"
     gene            complement(1740..2159)
                     /locus_tag="VNW28_03835"
     CDS             complement(1740..2159)
                     /locus_tag="VNW28_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HXA09086.1"
                     /translation="MRVYSASFTVSTGGKGTYEITAQVAAALAQSAVEHGTVTVFVRH
                     TSCSLIIMENADPTARRDLERFFERLVPEDTPWFEHTAEGGDDSTSHIRSVLTRASEV
                     IPIADGQMQLGAWQGIFLFEHRRAPHRREIALSVVGS"
     gene            2267..>2939
                     /locus_tag="VNW28_03840"
     CDS             2267..>2939
                     /locus_tag="VNW28_03840"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HXA09087.1"
                     /translation="MREKMSQQSTNAVVMIRPQRFYPNPETALDNAFQQKIVADSPDT
                     ISARAQTEFDCAVETLSSAGVAVHVFEDTPAPAKPDAVFPNNWFSTHHDGRVALYPMH
                     TPSRRPERRRDLIERLRERYRVTEVVDYSAYEKRGLYLEGTGSLVLDHIHRIAYVSLS
                     RRTDREPLEQFCADFDYEALTFTSSGDDGRPIYHTNVMMCLGSQFALVGLEMIDDRAQ
                     RETVRA"
BASE COUNT          562 a          934 c          895 g          548 t
ORIGIN      
        1 cgagggaaat cgtcacgccg accaccgagc agccgcaatt tccggcggca tacttcgcga
       61 gaccgcccca accgcaaccg attggttcta gacggcttcc cgagcggctg ccatacaaat
      121 cgaatcgctg caagacagag aaaccgatgc aaactaagat cgccgaagcc ctaacgaccg
      181 ccgaaatttt acgatgtttc ccggtcatgc gtcagctgcg gacgcacttc gaagacgggg
      241 agaggttcgt cgcgcaggtg gaacggcagc ggggcgaagg ctatcatctc gctttcctcg
      301 aagacgaaaa gggcgtccgc gccgtggccg ggtatcgcct ggcggaatcg ctccatgcgg
      361 gaaaattttg ctacgtcgac gacctcgtta cggacgcggc cgggcgttcg ttaggctacg
      421 gcggcgcgct tttcgcctgg ctggtggcgg aggcgcgcgc cgccggctgc ggaaagctgg
      481 aactggattc gggcgtgcag cgcttcgccg cgcatcgctt ttatctgacg aagcggatgg
      541 ttatctcctc gcaccacttt tcgctcgagt tgtagacgcg tcgctcggcg aaggagatcc
      601 tttctgcggg accagtctcg tgacgaagaa cacaactccc gcggcgcccg agggcgacgt
      661 ctacatcgcg cgtgccgaga tcagggcaag gcgaaataat ccgcgctctg gtagtggccg
      721 tcgaccgtct tcacgatctg catgagaatg tcgttgacca gggtgcttgc gccgaagcgg
      781 aaaagatcgg gcgtcaacca gtcgtcgccg agcgtttcct tcaccatcac gagataggcc
      841 agcgcctgct ttgcgttgcg cacgccgccg gctggtttca tcccgatgcg cacccccgtc
      901 tcgtaaaaat aatcgcggat cgcctccagc atcacgagcg tcaccggaat cgtggccccg
      961 ggtgtaacct taccggtcga cgtcttgatg aaatcgccgc cggcacggat cccgatttcg
     1021 ctcgcgagcc ggacgttgtc gtaggtctgg agttcgcccg tttcgaggat gaccttgaga
     1081 tgcgcctcgc cgcacgcttc cttcgtcgcg gcgacctcgt cgaaaacgcg attgtaatcg
     1141 ccggccagaa aaacgccacg atcgatcacc atgtcgatct catcggcacc gtcgccggag
     1201 gcgcgccgga cttcctccag tttcaaatcg agcggcatca gcccggtcgg aaaggcggtt
     1261 gcgaccgagg cgacttttac ccgagaagca ccaagcaacc gcttcgcgac gcggacgaga
     1321 ttgggataaa cgcaaacggc ggcgcaggac ggcacgtcgt aaagcggatc aaccggttgc
     1381 atcgctttgc ggcagagaaa ggccaccttc ccgggagtat cctttccctc gagcgtcgtc
     1441 agatccatca tcgagacggc aagtttcaag ccggtcagct tggccgaaga tttgatgctc
     1501 cgtttgccga aggcggcggc ccgttcctcg accatgacct gatcgacgct cgggatttcc
     1561 caacgagcgc gcagcgcttt gtggcggttg ttggaagcgg gcaatgcttt caagcggcaa
     1621 gagactaatg aagcgatggc aggatgtcga tgtgacggcg aacgttgccc ccggctcgac
     1681 aggagtctcg ccctgccgaa caatcgtgag cgggtcgata cgcctgcaag gcgggatggc
     1741 taacttccga ctacgctcag cgcgatctcg cgccgatgcg gcgcgcggcg atgctcgaaa
     1801 aggaaaatgc cctgccaggc gccgagctgc atctggccgt ccgcgatcgg gatcacttca
     1861 ctcgcgcggg tgaggacgga gcgaatgtgc gaagtgctgt cgtcgccgcc ttccgccgtg
     1921 tgctcgaacc agggcgtgtc ctccgggacg agccgctcga agaatcgttc gagatcgcgc
     1981 cgcgcggtcg ggtcggcgtt ttccatgata atgaggctgc aacttgtgtg cctaacgaac
     2041 acggtgaccg ttccatgctc gaccgcggat tgcgccagcg cggccgcaac ttgcgcagtg
     2101 atttcgtacg ttcccttgcc gccggtcgag accgtaaaac tcgcggaata aacgcgcatc
     2161 gccggcggcc acgctaaacg cagccaccgg cgaccgcaat cgtcctaatc gcccggcgcg
     2221 tccgttgtcc ggccgctggt ttgcccttag tttcgcgcga ccgccgatga gggaaaagat
     2281 gagccaacag agcacgaacg ccgtcgtcat gatccggccg cagcggttct atccgaatcc
     2341 cgagacagcg ctggataacg ccttccagca gaagatcgtc gccgactcgc ccgacacgat
     2401 ctcggcgcgt gcgcagacgg agttcgattg tgccgtcgag accttgtcat ccgcgggggt
     2461 cgctgtgcac gtcttcgagg acacgccagc gcccgcgaaa ccggatgccg ttttccccaa
     2521 caactggttc tcgacccatc acgatggccg cgtcgcgctt tatccgatgc atactccttc
     2581 ccgccgtcct gaacggcgcc gggatctcat cgagcgcttg cgcgagcgct atcgcgtgac
     2641 cgaggtggtg gactattcgg cctacgaaaa acgcggcctc tacctcgaag gcaccggcag
     2701 cctcgtcctc gaccacattc atcgcatcgc ctacgtttcg ctttcccggc ggactgatcg
     2761 cgagccgctg gaacagttct gcgccgactt cgattacgag gcgctcacct tcacgagcag
     2821 cggcgacgac ggacgcccga tctatcacac taatgtgatg atgtgccttg gctcgcagtt
     2881 cgccctcgtc gggctcgaga tgatcgacga tcgcgcgcag cgggaaaccg ttcgcgccc
//
LOCUS       DATMNS010000108         4640 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2298174, whole genome shotgun sequence.
ACCESSION   DATMNS010000108 DATMNS010000000
VERSION     DATMNS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4640)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4640)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4640
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2298174"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..580)
                     /locus_tag="VNW28_03845"
     CDS             complement(<1..580)
                     /locus_tag="VNW28_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXA09088.1"
                     /translation="MPQDSDFVAGLFVRDDDEGLFSRDINGQLVRLDSPTESDYEKNV
                     TLQIDGLPVTVPLAEPLTDANGNIVVDLEGRTTPRYTTIYDAARKLYVEQIGDEKKIP
                     IPVLCHQPHMAPVAVCRLCIVQIYGQKRGRRAAERKLLPACQHQVKEGMEVFTMNAPG
                     ADGDRVRQTVKVMTELLAADHLKPAKPPELEKE"
     gene            complement(584..2638)
                     /locus_tag="VNW28_03850"
     CDS             complement(584..2638)
                     /locus_tag="VNW28_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HXA09089.1"
                     /translation="MEIERLTLYAPVLVPALHRIQDEFGYLKPEALADFSRESGVPQY
                     RLQAVASFFPHFLLTPPKKIVLRVCRDLSCHLAGSGKMLRELDALTSEEVAVEGVSCL
                     GRCDRAPAACLSLQGAEHEQYYLARSSDELKAVVAAALQGAPPAPDHDFDRSFLGHDA
                     IIDPYKGEAPDYRAVRAAIAARDASLGAAIKLLSARREWTLGMLEKFRIAAIRQIRVD
                     FEMEPAIREAVRSWQTEDEWAQGPELGGWSEILLNEIREADLRGLGGAGIPAIQKWQD
                     VRDAVRKARQRESDDRAFIVVNADESEPGTFKDRELLLRTPHLVVEAVILASLITEAT
                     QGFIYIRHEYAEQIEACQREIRRAEQLGLCGENAMVLGRSLPISVFVSPGGYICGEQS
                     ALIEAMSDRRGEPRNMPPKLETNGLNDLPTLVSNVETFAWVPFVCLRGGKTYADQGVN
                     GWKGRRIFSVSGDVKRPGVYEVPMGITLRELICGEQYCGGIAGDKKLKGFAPSGPSGG
                     FLPAKLRAGNGLPRDHTNNKSWQALAARRGFDPAASELDILDLELELNLFRALSPTQA
                     LGAGLTVYAEDRDMAEQAVNSVEFFRNESCGKCVPCRLGSQKLASLGSHLLDGQIRAD
                     KWKNEILPLIKEMGTAIELASICGLGRSVPIPLKTAAAFFEEDVAKHLSGNGAARAGK
                     PG"
     gene            complement(2741..3904)
                     /locus_tag="VNW28_03855"
     CDS             complement(2741..3904)
                     /locus_tag="VNW28_03855"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXA09090.1"
                     /translation="MSDSAFYWRPTRVAIAVYLAGSLGATGSAAPLQTDQFVGAAGCK
                     SSSCHGGAGEKRSQYFTWVQQDFHGRAYAVLVDARSARMAETLSLPAAQTSERCTVCH
                     SPLQSVAPARLAKTVRVDEGVSCESCHGAAGPWLRGHTRKDWSYATRVTAGMHDLRNF
                     YVRANACVACHQNLAPDLLKAGHPELIFELDGQSVAEPEHWKDDDPARGPRAWLVGQA
                     VALREMSFALAQNETVESESLTRWQGLAWLLAKATATQSRLPLIAPPGESTSRDLLVS
                     VQQQADALARRAAVDPWNDVFAATALRALASADADFVPAKNISAGLLFRRAQRLVLAL
                     DRLSRATPQQPLGAPKNSALAPLFEDLRLQADFQPAKFAADLVTFRTTIERSP"
     gene            4120..>4640
                     /locus_tag="VNW28_03860"
     CDS             4120..>4640
                     /locus_tag="VNW28_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09091.1"
                     /translation="MRILNRAHVPWFLFVVLATSAAVWVYVGNFCPAYLPPGLGLPLA
                     LVQKVSEHRSIGGTPVGLIFGALSFAIFIFGALLSLRKRVVLWPLGSVQRWLRAHIWL
                     TLLTVPLVLLHSGFRLGGPMTTLLMVFYSVVMVSGIYGLCLQHRMPRIMQERLPAETV
                     YEQIPHVRAQLFRA"
BASE COUNT          868 a         1469 c         1398 g          905 t
ORIGIN      
        1 actctttctc caactcagga ggtttcgccg gcttcaaatg atccgctgcc aacagttcag
       61 tcattacttt gactgtctgc cgaacccgat cgccatcggc acccggagca ttcatggtga
      121 agacttccat cccctccttg acctgatgct ggcaggcggg cagcagcttg cgctccgccg
      181 cccggcgccc gcgcttttga ccgtagatct ggacgatgca aaggcggcaa actgccaccg
      241 gagccatgtg cggttgatgg cacaagaccg ggatcgggat tttcttttcg tcacctatct
      301 gctcgacgta aagttttcgc gccgcgtcgt aaatcgtcgt gtaacgcggg gtcgtgcgtc
      361 cttccagatc gaccacgatg ttgccgttgg catccgttaa cggttcggcc aggggcacag
      421 ttaccggcaa gccgtcgatc tgcagggtga cgtttttttc gtagtcggac tcagtcgggc
      481 tatccagccg gaccagttgt ccattgatgt cgcggctgaa taacccttcg tcgtcatcac
      541 gaacgaataa accggcaacg aaatccgaat cctgcggcat aggttatcca ggtttcccgg
      601 cccgggccgc accattgccc gacaaatgtt tggccacatc ttcctcgaag aaggcagccg
      661 ccgtcttgag aggaatcggg accgaccggc ccaggccgca gattgaggct aactcaatgg
      721 ccgtgcccat ctccttgata agcggcaaaa tttcattctt ccacttatcc gctcgaatct
      781 gcccgtcgag caggtgtgac ccgagcgagg ccagcttctg cgaccccagc cggcacggga
      841 cacatttccc gcatgactca ttgcgaaaga actcgacgga attaacggct tgctcagcca
      901 tgtccctgtc ctccgcataa acagttaacc cggcgcccag cgcctgggtg ggactgaggg
      961 cccggaaaag gtttagctcc agctcgagat cgaggatgtc cagttcagag gcggccggat
     1021 cgaagccgcg ccgcgccgcc aacgcctgcc agctcttatt gttcgtgtgg tcgcgcggca
     1081 ggccattgcc ggccctgagt ttagccggaa gaaatcctcc ggacgggccc gaaggggcaa
     1141 aacctttgag cttcttatcg cccgcgatcc cgccgcagta ctgctcgccg caaatcagtt
     1201 cgcgcagggt gattcccatc gggacttcat acacacccgg gcgtttgacg tctcctgaga
     1261 ccgagaatat tcggcgacct ttccaaccat tcacgccctg gtcggcgtag gtcttgccgc
     1321 cgcgcaggca gacgaaagga acccaggcaa aggtttccac gttgctcacc agcgtgggaa
     1381 gatcattcaa cccattggtc tccagtttcg gcggcatgtt gcgcggctcg ccacgccgat
     1441 ccgacatggc ttcgatcagc gcgctttgtt cgccgcaaat gtaacccccc ggactaacga
     1501 acacggaaat gggcaatgac cggccgagca ccatcgcatt ctcgccgcag aggccgagct
     1561 gctccgcccg ccgaatctcg cgctgacacg cctcgatttg ttcggcgtac tcatggcgga
     1621 tatagatgaa accctgcgtg gcctcggtaa tcagactcgc cagaatgacc gcttcgacga
     1681 ccaggtgcgg ggtgcggagg agaagctcgc ggtccttgaa ggtgcccggt tcgctctcgt
     1741 cggcgttgac cacgatgaag gcccggtcgt ccgactcccg ctggcgagct ttccgaaccg
     1801 catcgcgcac gtcctgccat ttttggatcg cgggaattcc cgcgccacct agcccgcgga
     1861 gatcggcttc ccggatctcg ttaaggagaa tctccgacca cccgcccaac tccgggcctt
     1921 gcgcccactc gtcttccgtt tgccaggagc gcaccgcctc gcgaatggcc ggctccattt
     1981 cgaaatcgac tcgtatttgc cgaatggccg cgatgcgaaa cttttccagc attcccaggg
     2041 tccactcccg cctggctgag agtaatttta tcgccgcgcc taacgaggcg tcacgggccg
     2101 cgatcgcggc ccgcaccgct cgataatcag gtgcttctcc cttgtaggga tcgatgatgg
     2161 catcatggcc cagaaaactg cgatcgaaat catgatccgg cgcgggtggc gcaccctgaa
     2221 gagcggccgc gacaacggcc tttagctcgt cactcgagcg cgccagataa tactgctcgt
     2281 gctcggcgcc ctgcagcgag aggcaggcgg ccggcgcacg gtcgcagcgg cccaggcacg
     2341 acacgccttc gaccgcgacc tcttcgctcg tgagcgcgtc caattcccgc agcattttgc
     2401 cggaaccggc caggtgacaa gacaaatcgc ggcagactcg cagcacgatt tttttgggcg
     2461 gggtgagcag gaaatgggga aagaaactgg ccacggcctg cagccgatac tgcggcacac
     2521 ccgactctct cgagaaatcc gcgagcgcct ccggcttgag atagccgaat tcgtcctgga
     2581 tgcggtgcaa ggcggggacg agcaccggag catacaaagt cagccgttct atttccattt
     2641 ttcttgtccg agtgccgccc cgaagatcgg ggcgcaggga gccggcgccg cgtgtccgag
     2701 aatccgggtt tcgttaggca tagagggcgg aacaatgcta ttacggcgag cgttctattg
     2761 tcgtgcgaaa agttaccaga tcggccgcaa atttcgcggg ctggaaatca gcttgcagcc
     2821 ggaggtcctc gaaaagaggg gcgagggcag aattcttcgg tgcgcccaat ggctgctgcg
     2881 gcgtcgcgcg cgagaggcga tcgagcgcga gaacgagtct ctgcgcgcga cgaaaaagca
     2941 ggcccgcgga gatgtttttg gcggggacga aatccgcgtc cgccgaagcg agcgcgcgca
     3001 gcgcggtggc ggcaaagaca tcgttccatg gatcgaccgc ggcgcgccgc gcgagcgcgt
     3061 cggcttgctg ctgcacgctg accaacaagt cgcggctggt tgattcgccg ggcggagcga
     3121 tgagcggcag gcgcgattgg gtggcggtcg ctttggccag caaccaggcg agcccctgcc
     3181 aacgcgtgag gctttccgat tcgacggtct cattttgcgc gagcgcgaag ctcatctcgc
     3241 gcaaggccac cgcctgcccg accagccagg cgcgcggacc gcgtgccgga tcatcgtcct
     3301 tccagtgctc cggctccgcc accgactggc catccaattc aaaaatgagt tccgggtggc
     3361 ccgctttcag aaggtccgga gcaagattct ggtggcaggc gacgcaggcg ttcgcacgaa
     3421 cgtagaagtt gcggagatca tgcatccccg cggtcacgcg ggtggcataa ctccagtctt
     3481 tgcgggtgtg tccgcggagc cacggcccgg ccgccccgtg gcaactttcg caggagacgc
     3541 cttcgtcgac gcggacggtt ttggcaaggc gcgcgggcgc gacggattgc aacggggaat
     3601 gacaaacggt gcagcgctcg ctcgtttgcg cggccggcaa agaaagggtc tcggccattc
     3661 gcgccgagcg ggcatcgacc aggaccgcgt aagcccgccc gtgaaaatcc tgctgcaccc
     3721 aggtgaaata ttggcttcgc ttttccccgg ccccgccgtg acaactggac gatttgcagc
     3781 cggcggctcc gacaaattgg tcggtctgaa gcggggcggc gctccctgtt gcgcccaggc
     3841 tcccggccag ataaacggcg atcgccacgc gggtcggccg ccagtagaat gcgctatcgc
     3901 tcacgctctc gaccttagcg gagagcgggg cggcaaagcc agcaaataac agaatcggcc
     3961 gccatctcgc ctaacgacgc aaaagttctt ccctgccaac gctggaagag tattctggcg
     4021 ccaactcccc aaggaaaaat ccgcatgcag gacgttgtcg aaaagctaac ccgcggtgac
     4081 ccgccgagcg tggcttcatt gatcgaggca tgaggtcgca tgcgaattct taaccgcgcg
     4141 cacgttccct ggtttctctt tgttgtcctg gcgaccagcg ccgccgtctg ggtctacgtg
     4201 gggaattttt gtccggcgta tctgcccccg gggctgggcc tgcccctggc gctcgtgcaa
     4261 aaggtgtcgg aacatcgcag catcggcggc acgcctgtcg gtctgatttt cggtgctctt
     4321 tccttcgcga tttttatctt tggcgcgctc ctcagcctgc gcaagagagt ggtgctttgg
     4381 ccgctcggca gcgtgcagcg ctggctgcgc gcccatatct ggctgacgct cctgaccgtt
     4441 ccgctcgttc tcctgcacag cggctttcgg ctcggtggac ccatgactac tctgctcatg
     4501 gttttctaca gcgtagtcat ggtgagcggg atctacggat tgtgtctgca gcatcggatg
     4561 ccccgcatca tgcaggagcg attgcccgcc gaaacggtct acgaacaaat cccgcacgtt
     4621 cgcgcgcagc tttttcgcgc
//
LOCUS       DATMNS010000109        24245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4809652, whole genome shotgun sequence.
ACCESSION   DATMNS010000109 DATMNS010000000
VERSION     DATMNS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 24245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24245
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4809652"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..1614
                     /locus_tag="VNW28_03865"
     CDS             268..1614
                     /locus_tag="VNW28_03865"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HXA09092.1"
                     /translation="MIIAVAVVMVILPVRRGVASPSPWSPRTLKKLALCLAILAIPSL
                     SFSQVPRGVFSIANSGKQPLASVLANPNVDGITVRQDWAALEPTDGNFDFSFLDSAIA
                     SCAASNKQVLLRIGTQAAKPAWVTNAVAAAGGSFFTFLDTSGTPTTIPVFWDPTFLAK
                     KTAMIAALGAHLANNPTVTIVSASFANAISEDWNVPHSPTDVTNWLALGYTSDKLLAA
                     GQQIIDATMIAFPNQIVTMAVGGSGHVNGINLDPTNDYAARAAVGTARASWPGRFNVQ
                     KNDLSTFIPAAPGTDSLYEMIWDFQPDVAGQMVFQCANDPTYRANNGVPQDPSITLEQ
                     SIDNAVSYNEKYVEVYQTDANSLLDAIAYAHAALTADPSPTPTPTPSPTPTPSPTLTP
                     TPTPSPTPTPTPTPTATPTPTPSPTPTPTPSPTPTPSPTPTPANAPSPPTGLHVVF"
     gene            2565..3407
                     /locus_tag="VNW28_03870"
     CDS             2565..3407
                     /locus_tag="VNW28_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09093.1"
                     /translation="MVQAAPVISAYGAPPEFSQSRFGPLTNAYVLPAGAVYTSVIYEN
                     DSAAFRKPDNTFTQELEIGLPYRFNVAAEHEVQYYDGTLQETSCSLEARYAFADWNKI
                     PLNPTFFTELKFGVGNILHDEGAPTPGRNFGPGGFDNTQHLPDSSEMRLLLSQEFFGR
                     IEWALNSFFEQELGGDRGREWGLAQSIETPVILPNEALKVGIEAQFRSFSDKTSRGQP
                     YNSVVVGPTAGWKPTRNTRLDISPLFGVNEKSPHVQGFVVFSVLLGGGEGQEAEAPTS
                     TRNR"
     gene            complement(3600..3674)
                     /locus_tag="VNW28_03875"
     tRNA            complement(3600..3674)
                     /locus_tag="VNW28_03875"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3640..3642),aa:Val,seq:gac)
     gene            complement(3843..4394)
                     /locus_tag="VNW28_03880"
     CDS             complement(3843..4394)
                     /locus_tag="VNW28_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA09094.1"
                     /translation="MHNLLTVKETAKYLRIPLPTVYYLVQRGQLPAIQIGGRWRIKKD
                     SLDKDVLKEDRSGQPTVLVVDDDVSLQNLFKVFLKKIGFSRVVVGTVKEAIAALEKQR
                     FDLLFLDLKLPDGPADDVYDAAKEIDPNLPIVVITGYPDSEMLNRILAKGPITVLKKP
                     LKVDQLKQTVRILGHKEATKLAA"
     gene            4609..5232
                     /locus_tag="VNW28_03885"
     CDS             4609..5232
                     /locus_tag="VNW28_03885"
                     /inference="COORDINATES: protein motif:HMM:NF025033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine N-6-methyltransferase family
                     protein"
                     /protein_id="HXA09095.1"
                     /translation="MSALFFKRFLQKPFQIASIIPSSKALVERVASKMDFSQPRIMAE
                     YGPGEGVHSRELAARMDADSQLFLFELDRAFSRDLERQFADDPRVHIINRDAASLAEE
                     LARRGIGQCDYILSGIPFSILEIKKKRALLQDTYDALRPGGAFIIYQVTNELRQHATF
                     FDRAESEYCLQNIPPMFITVFQKALTRNGHVRVAPLPTAKITAQRMA"
     gene            5250..6647
                     /locus_tag="VNW28_03890"
     CDS             5250..6647
                     /locus_tag="VNW28_03890"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008909.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXA09096.1"
                     /translation="MEKDSMGEMSVPESALYGASTQRAVLNFPVSGWRFSRPFIRALG
                     LLKWAAAQANHDLGLLDAERSALIVQAAEEVVEGKLDEHFPLDIFQTGSGTSTNTNAN
                     EVISNRCCQLAGQPIGARLPVHPNDHVNMGQSSNDVIPSAIHISAAEQLKTCLIPSLG
                     KLEAALAAKATEFHDVIKIGRTHLMDATPVRLGQEFGGYAQQVRFGKRRAEQSIAALQ
                     ELALGGTAVGTGLNRHLGFPAKVMRHLEQRTGIAFREAENHFEAQGAKDGVVEVSGQL
                     KTIAVSLFKIANDIRWLGSGPRCGIGEIALPATQPGSSIMPGKVNPVICESVMMVCAQ
                     VIGADTTITWAGANGNLDLNVMMPVMAHNLLHSIRLLGNVAEIFADKCVSGILANKER
                     CQELVELSMAMVTSLAPKIGYDRAAEIAKESARTGKTVRQICREEKVLPEAELNAALD
                     PIAMTEPGGEGGGGG"
     gene            6711..7085
                     /locus_tag="VNW28_03895"
     CDS             6711..7085
                     /locus_tag="VNW28_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXA09097.1"
                     /translation="MVANVNSPNFSGGANIAIKCPYHTYEQTIAFYRDTLGLPVVEEE
                     AEGCIFQFGPVRLWIDRVPNISHPDIWLELETNDTESAAAYLKNHGVPRRDEVEQLRE
                     DFDGFWISDPAGVIHLVVGDED"
     gene            complement(7102..7983)
                     /locus_tag="VNW28_03900"
     CDS             complement(7102..7983)
                     /locus_tag="VNW28_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXA09098.1"
                     /translation="MFSLTMLGSGSAGNSALVATSHCRLLVDGGLSARQLVLRLARCG
                     FTPNDIDGVLLTHEHGDHVCGLEVLCRKFEVPIYCNALTAEAIRYPASAGLGAHRNWR
                     IFRTGSSFSICDVTIENFSVPHDAVEPVGYTFHAGTSALGYITDLGHATRLTIERLRQ
                     VQTLVIETNHDEKLLQNDPHRPWPVKQRIQSRHGHLSNTAAAAAIAQLLPGKVARVVL
                     GHLSRDCNSPELAAGTVRAELEKCGRADIEVFCATQAEISPQFAIGATRGGAFQPTFE
                     NLFFETTAVSLGAPRTA"
     gene            8096..8746
                     /gene="pdxH"
                     /locus_tag="VNW28_03905"
     CDS             8096..8746
                     /gene="pdxH"
                     /locus_tag="VNW28_03905"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HXA09099.1"
                     /translation="MDAIDLAGLRREYETQGLRRAELHHDPIEQFATWFSTALNSALP
                     DANAISLATATPGGKPSVRVVLLKGFDQRGFVFFTNYESDKGRELASNPQAAFVVYWV
                     QLERQIRVAGRVEKTSRKESETYFHARPRGSQLGAWVSRQSEVIDARRILDARLAEMT
                     ERFAGSNEIELPPHWGGYRIVPETMEFWQGRANRLHDRFRYTRQKDASWLIERLAP"
     gene            9024..9314
                     /locus_tag="VNW28_03910"
     CDS             9024..9314
                     /locus_tag="VNW28_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09100.1"
                     /translation="MGGVLLLHDMSRFAQAFDVTAQGGVRRACDEWREGNPQAEAICL
                     NGESRTMELPSPVYKDACGLGILHKSGVPAPPNGDTGTAVSLTNQKHIETPP"
     gene            complement(9364..10488)
                     /locus_tag="VNW28_03915"
     CDS             complement(9364..10488)
                     /locus_tag="VNW28_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09101.1"
                     /translation="MLQKSFARSVVPRPRILFTLGLWAASCLGAIYPANTLLAQDAVT
                     SNKEPLTVATTTSNSTSTAKKPASAASPASASTNQTAAVPEQETMVIRGEEVPSAYGA
                     PPALSRSRFSNLVNAYVLPPGAVYAGFIYEGDALKFDRPDHNFTEEVEIGLPYRFGVA
                     FENAVETYRGTTQERSFSVEARYALADWDKIPFNPTLFVEYKFGIGDILHDEGPPAKP
                     GPGEGRDFLDMHLPLPDAVEGRVLFSEEFAEKLEWALNVFYEQEVGGDRGREYGFAQS
                     AMIPVLLPKERLKVGAEMQLTVFSDKGIREDPATGESTRFIIGPTVAWKPTKNTRLDV
                     SPLFGVNTDAPRAQVFAVFSYLFGGHEGNEAEAPASTRNR"
     gene            complement(10577..11992)
                     /locus_tag="VNW28_03920"
     CDS             complement(10577..11992)
                     /locus_tag="VNW28_03920"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HXA09102.1"
                     /translation="MSSKSTEPRSIASQLVFLFTPAAALLLCCGLAVLYWIVVRHAFE
                     EDRAVLVDKVLAIRAELTTAGEPRLLENELKTLRGGERAAYWVRVVDSAGATVAETPG
                     MKRLLPPELFTRGRNAGVSENGPFDFRTAGKLFSLVSAAAVVHGQPYTIQVAQDRSED
                     EGFMLEFGFLVAAVLACGTFASVVIAITVTKRGLQPLAAMTRSLQRVGPHQLHERVPP
                     AEWPRELQPVAIAFDEMLDRLEDSFTRLSQFSADLAHELRTPLANIRGEAEVALARPR
                     SPDEYQAVIESSVAECARLSGIIDNLLFLARAEAAERQAQCSLFDGRAAIEKIAAYNE
                     AIAEERRLVIRCEGEGEVYADPVLFGRAVSNLVDNAVRFTPNGGQIVMALAVGTDGAQ
                     IVVRDSGCGIAAEHIPRVFDRFYRVDRSRSSEGTGLGLALVKSIADLHGGSLSVVSEL
                     GRGTVVTLRFPSPALVNGASD"
     gene            complement(11973..12644)
                     /locus_tag="VNW28_03925"
     CDS             complement(11973..12644)
                     /locus_tag="VNW28_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HXA09103.1"
                     /translation="MHILLIEDEKKTAAFIAKGLEEEGYSVESASDGETGLQLALATR
                     FDLLLVDVMLPKKDGWAVVEELRSGGAHTPILFLTARDSVPDRVKGLELGADDYLVKP
                     FAFSELVARVRSLLRRSPAQQGEQLRIEDLELDTRRNRAVRAGDPLHLTAKEFLLLAQ
                     LVRSAGEVVTRKDIESHVWGIDFQTGTNVLDVMVRRLRAKVDDPYSRKLIHTVRGAGY
                     VLKVD"
     gene            complement(12806..13006)
                     /locus_tag="VNW28_03930"
     CDS             complement(12806..13006)
                     /locus_tag="VNW28_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09104.1"
                     /translation="MGLSCRQRPPQNSLTKNKMIECVRSWDVVAQRHGSGFVRARETA
                     RDMIAAGEGKNVPRSGPHGGDL"
     gene            13087..13827
                     /locus_tag="VNW28_03935"
     CDS             13087..13827
                     /locus_tag="VNW28_03935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09105.1"
                     /translation="MKNYLRLNLSLLYWIFVAPFRGEMFELAPVFRQMVAIGVRAAPM
                     VALTACSVGVTLAMQGAHSLQQLGAEIYVPDLVMVTLLREMGPVLIAVIVIGRSCSAV
                     AAELGTMKVSEEIEALEVMAINPISYLIVPRFLAMLVMLPALTILGDYIGVFGGWAVC
                     RLALDFDTAGYVLRAVQSADTWDLYSGMIKSVVFAWIVITIACNAGLHVEGGAEGVGQ
                     ATTSSVVQALLAMLVTNAILTSIFFFGA"
     gene            13824..14678
                     /locus_tag="VNW28_03940"
     CDS             13824..14678
                     /locus_tag="VNW28_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA09106.1"
                     /translation="MTPENQPVVEVKNLIRKFGERSVIDDLNFQIDRGETLVIMGGSG
                     CGKSTLLRHIVGVMKPNSGSVQIFGEEITTMNDREIARVRRRFGMLFQSGALLASLTV
                     GENVALPLLQHTDLSPAEIDEVVTRKLQMVGLTGFENLKPAEISGGMKKRVGLARALA
                     LEPELLFSDEPTSGLDPIMTAVVDRLTLELTRGSGMTAVVVTHDMTSAFRIATRMIML
                     NHGKIIAQGTPNEIRANPNPDVQQFINGEADGPVPMSLSQSEHQNSAHVQPRPLVGAR
                     GRFHRKTS"
     gene            14675..15577
                     /locus_tag="VNW28_03945"
     CDS             14675..15577
                     /locus_tag="VNW28_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXA09107.1"
                     /translation="MKRNFSDYLVALAVVACSLVLLGALTFALSGYRLQKPSRVLQID
                     YEDVTGIKVHSEVRYAGAPAGRVIAMRHLSAAQRVAEPNKKDAVRVSIELLDSVPPLP
                     SDIAASLSSDSMLSPKFVSLSAGTPGKAVLPNNAIIEGAPAYGIDQIIGAVGPVLTNA
                     NKLIDNLNGTATNLNGTVTIVKTDLGLFLPKLSPVADTAKVDLEELQKTIQGLNGVEA
                     KASNVFATADTFVGSTDQQLREQMRELHVILLNLKVITTHAKALVDTLAEKPNRIIFS
                     GKANKLTPEDQIIKSHQALPARTP"
     gene            15644..18463
                     /locus_tag="VNW28_03950"
     CDS             15644..18463
                     /locus_tag="VNW28_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09108.1"
                     /translation="MVIFQRTAVGFAFLVWFAELVPPARAQESETAPLVSPPAAVATP
                     TSSPVPSATAAPSPSPVQLTDILAKGVKARVIGPAVMGGRVSDIAIDPRNPAVFYVGL
                     GTGGVFKTGNNGVSFDPIFDKQPVLSIGAVAIAPSDSDVVWVGTGEANDRNSSEWGDG
                     VYRSTDGGGAWTNVGLKESRAIARIVVHPTKPEVAYVAAMGNLWKDGGERGLYKTTDA
                     GKTWKLVLHAPVPNDARSGCGDVALDPSNPEIVYAALYARQRTPWSFAYGVTATNGFD
                     VGGIFRSSDGGASWKKCAGGLPGLTGRIGLAVSASKPKLVLAIVQSDEGGASDISDLH
                     SRRGGVFRSEDGGGKWTRMSDINPRSFYFSQIQVDPSNDQRVYVLGMAVLVSDDGGKN
                     FREDLSEKLHPDCHALAIQPGTAPSPKRAKPEDKNKPPKAPVCQRLLLGTDGGLYQSY
                     AAGKAWDHLNRFPAGEFYRISLDDTQPFFRIAGGLQDNESFVGPSQVPSKEGIRNSDW
                     TALEGGDGFYVVFDPSDRDIFYAESQEGYIHRINLRTGEKRDLRPEAAEGQERYRFHW
                     NAPLIGSRDQPGVLYLAGNRVFRLTDRAEHFQVISPDLTHHDPARVNTSGSGAENYGV
                     VYSLAESPAKAGLLWAGTDDGRLWLTQNDGANWAELTSKLPEPARGQWIVRIEPGAKD
                     PKVAYVVTNAYRRGDDRPFILRTADLGQTWQSVIGQGLLPNDPVEVVREDPVNSNLLY
                     AGTHFGLFASFDGGGHWLRVGDLPNVRVDDLQIHPRSADLVIATHGRSLEILDDTRPF
                     RELTPEVMAQLAHLFTIAPARGFYLLAGSADWNGKGIYRGENPPEGALITFWVKEFTG
                     DEVKIAITNSAGQPVANLKAAGAPGLGRVSWDLRPTEDVRIKYGGDDPKKFVASDDYT
                     AELSYGKEKMKQTFHVEIAEGIVTR"
     gene            18505..20544
                     /locus_tag="VNW28_03955"
     CDS             18505..20544
                     /locus_tag="VNW28_03955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA09109.1"
                     /translation="MSQAGTTVMREKTIAGVGGALLLVALLAAYANHFHNGFHMDDGH
                     AIVDNVSIRALRNIPLFFRDATTFSALPSNQSYRPLVSTLLAIDYRLGHGLQPFWFHL
                     STFILFLALTLLLAFVIHHLLALGAASSPCPWIALAPAAYYALNPANADTVNYLIASS
                     EVISALGVIASFAVYFAFPRCRQYFLYVLPAAIAILAKPTAAIFPVLFAVFRLLFRGP
                     AVAGPRPRPRSRLALEIVPPFVICGAMLWFVQDMTPRNWVAGAANAHNYLITQPYVAL
                     LYCKTFFWPTGLNADYDLNPFVTTGDVRLWIGFVFVVLLSAAAIVAAVGKRTRLIGFG
                     LLWFLLALLPTSLFPLAEAMNDHRAFLAYIGLVIALAGAAALLVARLDRPRRWTKIAA
                     GCAVALFLCANACGTFQRNEVWKTEETLWHDVVLKSPRNGRGLMTYGITLVNNGNFAG
                     ALDYLRRAQPLTPPYSVLLINLAMAENATGEIATAEQHFKDALRLAPLSPDSYIYYAR
                     YLLAHARVDEAQTLLRRALELSPTNLTARELLQKAGTHEVEAHATEGDALLRQGRFGE
                     AITQYEAVLEIAPDFVATLNNFAWLLATCPDGSLRDGARALELAKKADRLAGGGSPVF
                     LRTLAAACAENGRFKEAVETAQRAAQIALAQKNFVLAKKLEKDVELYRNNLPLQR"
     gene            complement(20620..21204)
                     /gene="infC"
                     /locus_tag="VNW28_03960"
     CDS             complement(20620..21204)
                     /gene="infC"
                     /locus_tag="VNW28_03960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXA09110.1"
                     /translation="MLQQQIRVNGRIRAREVRVILASSSEQLGVMKLSEAIKKAQGMG
                     LDLVEVAPTANPPVCKIVDFGKFRYDISKQEKDKKTSGTKLKEIKFRVNIDDHDYLTK
                     IRHAEQFLDKGNKVRVQLQFRGREMAHQEFGMQLMFKVRDDLATMAQVEMEPKTAGRN
                     ITMTLSPLPANKRKRRFSTENEQIEEDRDADSED"
     gene            complement(21446..22102)
                     /locus_tag="VNW28_03965"
     CDS             complement(21446..22102)
                     /locus_tag="VNW28_03965"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HXA09111.1"
                     /translation="MRLTITNYLDVISSWCHWAIPAWSELREKYGDRVDFHWKVALMD
                     KKGLPPTRAHTEWYYRRSGMLMRSPQMLHAGWVKPDSAEYLAPNCVAEAARDFGVVDD
                     RVWIAISTAGLRQGKRFTHWDVAAEVGAEAAGLEPEHLLEHAKTPAIEARVRQSTAEF
                     HALQVTQRPTFVIDTPTGDRAIFSGFAKAAPLAATLDTMLDDIVGYRAHAAHFGDPPA
                     "
     gene            22212..23774
                     /locus_tag="VNW28_03970"
     CDS             22212..23774
                     /locus_tag="VNW28_03970"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXA09112.1"
                     /translation="MVDLNHILLFVAVVSPLILLARIAQLRNPRNAGWRIAAVIVLAA
                     CALAWWFLPSIAGFVGGTFWCLLLLIPSVAERKIDDLLVAQRFARARQLAVVRRILHP
                     WEDSPQRPALLDCLALASSGRLDRALDQLATERAAPTPAGSFASALTFALTENWAGLV
                     QWCRRDLSVTANPAVLSLYFRALGETGALEELVRQFASRRASREPELTPDALPRFNFA
                     LVLAFAGRTNDLVRLFAGDLRQMPLERRQFWLATAELAAGDGRAARERLEKLRAETRD
                     AVLRRSVDRRLGQAPEFPPFSAAGAGLLARLVVETMGTQQTGTRPRTSGTPAVWGLIL
                     LNVAMFGAEWIFGGTTNTRTLHNLGALEPDAVIRQHEYWRLLTALFLHYGALHISFNL
                     YALYLLGPELERMIGSIKFTISYLLAGLGSSAGVVLLRALDLTNANLLVGASGCVMGV
                     IGISAGLLLRHRQSPLAGRRLRNIVVIVVLQTAFDLSTPQVSLAAHLSGFVTGVAIGM
                     ILARQRRRAVPF"
     gene            23869..>24245
                     /locus_tag="VNW28_03975"
     CDS             23869..>24245
                     /locus_tag="VNW28_03975"
                     /inference="COORDINATES: protein motif:HMM:NF023325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3417 domain-containing protein"
                     /protein_id="HXA09113.1"
                     /translation="MPNIQTYNVIPNLPAQLEPLREMVFNLWWTWEPSARRLFRHLDP
                     GLWERTNHNPVRMLQLSRQARLLEVAKDEVFLRELAEVHGAYKKYLARKDTYGKKGAG
                     NEIKKPIAYFSAEFGFHESIPNYS"
BASE COUNT         4695 a         7475 c         7213 g         4862 t
ORIGIN      
        1 cctgattcgg ggcggcgaac gacttcaatt acagatgctt cactcggtct gatgattcga
       61 agattctggg agattgtaat cgatttgtaa agcaaatttc tgtttccgga gcggatccgg
      121 gggcttgttt tccccccgga accctttgtt agaactgagg cagccaagaa gcggttcgcc
      181 gggccgattt ttccgtggtc cgctcgctct cgcgttcatg attcccagcc gcttcgttcg
      241 aaaccccggc aggtggccga ttgtggtatg attatagctg ttgcagtggt tatggtaatt
      301 ctacccgtac ggcgtggtgt cgcgtcgccc tccccttgga gccctaggac tttgaagaag
      361 ttggccctgt gcctggcgat cctcgcgatt cccagcctct ctttcagcca ggtcccacgc
      421 ggggtctttt cgattgcaaa ttccggaaaa caacctctcg cctcggttct tgccaatcca
      481 aacgtagacg gcatcacggt ccggcaagac tgggccgcac tcgaacccac agacggcaat
      541 ttcgacttta gttttcttga ttcggcgatt gcgtcctgcg ccgcttccaa caaacaagtc
      601 ctgctgcgca tcggcacgca agccgccaag ccggcttggg taacgaatgc ggtcgctgcc
      661 gccggcggca gcttttttac gttccttgat accagcggca caccgacgac aatccccgtc
      721 ttttgggatc cgacattttt ggcgaagaag accgcaatga tcgcggcctt gggagcacac
      781 ttagccaata atccgaccgt cacgatcgtg tcggcgagct tcgccaatgc catcagcgag
      841 gactggaatg ttccccattc cccgaccgac gtgaccaact ggctggcctt gggctacacc
      901 tccgataagt tgctcgccgc aggccagcaa atcatcgacg ccaccatgat cgctttcccc
      961 aaccagattg tgaccatggc tgtaggcggg agcgggcacg ttaacggaat caatcttgat
     1021 ccgaccaacg attacgccgc ccgggcggcg gtgggaacgg cccgcgcctc gtggccgggg
     1081 agatttaacg tgcagaagaa cgatctttcc accttcatac cggctgcgcc gggaactgac
     1141 tcgctttacg agatgatctg ggattttcaa cccgatgtcg cgggacaaat ggtctttcaa
     1201 tgtgccaatg accccaccta ccgggcgaac aacggagtgc cgcaagaccc gtcaattaca
     1261 ttggaacagt cgatcgacaa tgccgtgagc tataatgaga aatatgtcga agtctaccag
     1321 accgatgcca actccctcct cgacgcgata gcttacgcgc acgctgcgct caccgccgat
     1381 ccgtctccga caccaacccc aacgccttct ccaacgccga caccgtctcc aaccctgaca
     1441 ccaaccccaa cgccttctcc aacgccgaca ccaactccaa ccccgacggc aaccccaacg
     1501 cctacaccgt ctccgacacc aactccaacc ccgtctccaa cgccaacgcc ttctccaact
     1561 ccgacgcccg cgaatgcacc gagtccaccg acagggctgc acgttgtctt ctaggaacgg
     1621 accagagtcg cgttcgctcc atttttgggc tttggcttcg tgtcggtggc cgctcgggag
     1681 agggatggat taccttgcct cgcgccggga gtcagttcct cgaattcgcg gatgagtgcg
     1741 tcgtccgcga atatgcggcc ccggctgttg tcgacgtagc ttcgccgata aattcctcgc
     1801 catcgcgggc gaagatgaaa taagggcgca cccggtggcg tcgtcgccta acgaccgaac
     1861 gactccgcgt ctcatagcgc ggcggcggcg tcatgccaga aattgcgatc aggacggacc
     1921 ggcgccgctt tcctgcatgc cgaatgacga caccctgctc ttcgcggagc acgtccaaga
     1981 aatggaggcc agcgcccgta tgaccggtct ttcgtttgct ttcttaccga tgtggccgtc
     2041 atgcaaaagc gcgcgtgtcg cggctacgcc aataattcgc tttgtccggc cgccggagtc
     2101 agaatcagag gtctgatatc ggtgctgcca ttccggcagt gcgacatgac ttttgcgaaa
     2161 gggaggcgat gagtttacaa ggccccctag cgatgaaacg cgggttccaa actgctggat
     2221 tccatgacgc caagattaac gaagcctatc taatttgatg acgatatgac gaaacggtaa
     2281 gaaatgggtt gtcttgtccg tgcggtttct actacagccg ttacatgtca aatccacacg
     2341 gtatcgactc agtcacacgt cgcttcttaa gaatcgctgt cgttttcttc tgtctctcgg
     2401 gcgcaatggc gctgctcagc gagagtccgt ccgccttggc atccgctccg gcaaactcca
     2461 gtccgacccc gagcccgacg ccttggaaag ccaagtcagc tgaatccacc gccgcgtcgg
     2521 gtcagtccga acgaacctca aggacaagca gccagcagac gatcgtggta caggctgcgc
     2581 cggtaataag tgcctacggc gcgccgccgg agttttccca atcgcgcttc gggcccctga
     2641 ccaacgccta tgtccttccg gcgggggcag tttatacgag cgtcatttac gagaatgatt
     2701 ctgcggcctt tcgcaaaccg gataacactt ttactcaaga attggaaatc ggtcttcctt
     2761 atcgctttaa cgtcgcggcg gagcacgaag tgcagtatta cgacggcact ctgcaggaga
     2821 ccagctgcag tcttgaagcc cgttacgctt ttgcggactg gaacaagatt ccgctcaatc
     2881 caactttctt cactgaatta aagtttgggg ttggtaatat cctccatgac gaaggcgcgc
     2941 cgacgcctgg gcggaacttc gggcccggag gtttcgataa cactcagcac ctgccggact
     3001 cgagcgaaat gcgccttttg ctttctcaag agtttttcgg ccggattgaa tgggccttga
     3061 attctttctt cgagcaggag ttgggcggtg atcgcggccg ggagtggggt ctggcccaat
     3121 cgatcgagac gccggtgatt ctacccaacg aagcgctgaa ggttggcata gaggcgcaat
     3181 tccgaagctt cagcgacaag acgagtcggg gacaacccta taacagtgtc gtcgtgggac
     3241 caacggcagg ttggaagccc acgcgcaaca cgcgcctcga tatctcgccc ctctttgggg
     3301 taaacgagaa atcgccgcat gtgcagggct tcgtcgtttt ctccgtgctc ctcggtggtg
     3361 gagaaggtca ggaagcggaa gcgccgactt cgacgcgaaa ccggtagaac tcctgacaac
     3421 tcgggcggct tgatccaaga gctgaaatca cgaaaagaga gatcagaaat tcagcggccg
     3481 gaggatgccg aagacaaggg agagattgcg tcccgcaacc cagccgccgc aggcgaggag
     3541 gtagcgttcg atttggcgcg caatcaggct cgattgaaaa tcgcctctgc ttgttgagat
     3601 ggtgcgcgat acagggttcg aacctgtgac ccctaccgtg tcaaggtagt gctctaccac
     3661 tgagctaacc gcgcaggcgg gcgaaagcac aagagacgaa tcgcgtgcct cgagtcaagc
     3721 gcgaagaagg atgcgccttt gaagcaagcg ggaacgcaag gccgcagcga agtcggtcgg
     3781 accctaactc cggtggctca ttcggccgct accaggcctg ggagcggaat ggcccgcccg
     3841 gatcaggccg cgagtttggt cgcttctttg tggcccaaga tccgaaccgt ctgtttgagt
     3901 tggtcgacct taaggggctt tttcaaaaca gtaatcggtc ctttcgccag aatccgattg
     3961 agcatttcac tgtcgggata accagtgatg acgacgattg gcagattggg atcgatttcc
     4021 ttcgccgcgt cataaacatc gtccgctggt ccatccggca atttcaggtc gagaaagagc
     4081 aaatcaaatc gctgcttctc gagcgcggca atcgcctctt tgaccgtccc gacgacgacg
     4141 cggctgaaac caattttctt caggaaaact ttgaagaggt tttgcaggct gacatcgtcg
     4201 tccaccacca acaccgtcgg ctggccggaa cgatcttctt tcaggacatc cttatcgagg
     4261 gaatctttct tgatccgcca acggccgcca atttgaatcg cgggcaactg gccgcgctgg
     4321 accaaataat agacagtcgg cagcggaatt cggagatatt tcgcagtctc cttgaccgtc
     4381 agtaggttat gcatggcagg aggtcagcaa tttttttcgc cagttgcaat atttattgcc
     4441 gaaacatgag cattataaat tttagaatgc gtgtcaacca tcttatttca cgtggcagaa
     4501 gaggcttcgc gcttggaaag aagcccgatc gctgggggcg gaactctcat cctgcagcga
     4561 tatcgccgct ggacattggg tgaccggggg ttcactattc gcaactttat gagcgcattg
     4621 tttttcaaac ggtttttgca aaagccgttt caaatcgctt ccatcatccc cagttcgaag
     4681 gcgctcgtgg agcgggttgc gagcaaaatg gatttcagcc agccgcgaat catggccgag
     4741 tatggtcctg gagaaggagt ccattcccgc gagttggccg ctcggatgga cgccgactcg
     4801 cagcttttcc tcttcgaact ggatcgggcc ttctcccgcg atctcgagcg gcagtttgcc
     4861 gatgatccac gcgtccacat catcaaccgc gacgccgcca gtctcgccga ggagctcgct
     4921 cgccgcggca tcggacaatg cgactacatt ctttccggca ttcctttcag cattctcgag
     4981 atcaaaaaga aacgcgctct gctccaggat acctacgacg cgctccgacc cggcggcgcg
     5041 ttcatcattt accaggtgac aaacgaactc cggcagcacg ccaccttttt cgaccgcgcc
     5101 gaatccgagt actgcctgca aaatatcccc ccaatgttca tcacggtttt ccaaaaagcg
     5161 ctgacgcgga atggacacgt gcgggtcgcg ccgctgccca cagcaaaaat tacggcccag
     5221 cgcatggcat gagtgcctgc aagactcgca tggaaaagga ttccatgggc gagatgtccg
     5281 tcccggaatc cgcgctttac ggagcttcga cccagcgcgc ggtgctgaat tttccagtca
     5341 gcggctggcg tttttcgcgg ccgttcatcc gcgcgcttgg tttattgaaa tgggccgcgg
     5401 cccaggcgaa ccatgatctt ggtttgctcg atgcggagcg cagcgcgctc atcgtgcaag
     5461 ccgccgagga ggtggtcgag ggcaagctcg acgaacattt tccgctcgac atcttccaga
     5521 ccggctcggg gacgagcaca aacacgaacg caaacgaagt catctccaac cgctgctgcc
     5581 agttggccgg ccaaccgatc ggcgcccgcc tcccggtcca ccctaacgat cacgtcaaca
     5641 tggggcaatc gagcaacgat gtgatcccat cggcgattca catcagtgcc gccgagcaac
     5701 tcaagacctg tctcatcccc tccctcggga aactcgaggc cgcgctggcc gccaaagcga
     5761 ccgaattcca cgacgtgatc aagatcggtc gcacccattt gatggacgcg acgccggtgc
     5821 ggctcggtca ggaattcggc ggctacgcgc aacaagtgcg ttttggaaaa cgccgggccg
     5881 agcagtcgat cgccgcgctc caggaactcg ccctcggcgg cactgcggtc gggaccggcc
     5941 tgaaccgtca cctcggattt ccggcaaaag tgatgcggca tctcgagcag cgaacgggca
     6001 tcgcatttcg cgaagcggaa aatcatttcg aagcacaggg cgccaaggat ggcgtcgtcg
     6061 aagtgagcgg ccagctcaaa acgatcgcgg tcagtctctt caagattgcc aacgacattc
     6121 gctggctcgg cagtggtccg cgctgtggga ttggcgaaat tgcgctaccc gccacccaac
     6181 ccggcagctc catcatgccg ggcaaagtga acccggtgat ctgcgaatcg gtcatgatgg
     6241 tttgcgcgca ggtcatcggc gccgacacga cgatcacctg ggcgggcgcg aacggaaatc
     6301 tcgacctcaa cgtcatgatg ccggtgatgg cgcacaatct cctgcacagt atccggctgc
     6361 tcggtaacgt ggccgaaatt tttgccgaca aatgcgtcag cggaatcctt gccaacaagg
     6421 agcggtgcca ggaattggtc gagctctcga tggcgatggt gacgagtctg gcgccgaaaa
     6481 tcggctacga tcgcgccgcc gagatcgcca aggaaagcgc gaggacgggc aagacggtgc
     6541 gtcagatctg ccgggaagag aaggtcctgc cggaagcgga attgaacgcc gcgctcgatc
     6601 cgattgcgat gaccgaaccg ggcggggaag gtggcggcgg aggttaagtt tcccgggagt
     6661 tcgtttgcct aacgatcggc cttcgccaat cgaaattatt ttttcttccc atggtcgcca
     6721 atgttaacag cccgaatttt tccggcggcg cgaatatcgc gatcaagtgc ccgtatcaca
     6781 cctacgagca gacaatcgct ttttatcgtg acaccctcgg cctgccggtc gtcgaagagg
     6841 aagcggaagg ctgcatcttt cagttcggcc ccgtccgcct gtggattgat cgggtcccga
     6901 acatcagcca tcccgacatc tggctcgagc ttgaaaccaa cgatacggag agcgccgcgg
     6961 cctatctcaa gaatcacggc gttccacggc gcgacgaagt ggaacaactg cgcgaagact
     7021 tcgatgggtt ctggatttcc gatccagccg gcgtcattca tctcgtcgtg ggcgacgaag
     7081 actaactctt tgcggtcaga attacgccgt ccggggcgcg cctaacgaga ccgccgtcgt
     7141 ctcgaaaaaa aggttttcaa atgtcggctg gaacgcgccc ccgcgggtcg cgccaatcgc
     7201 aaactgcgga ctgatctccg cctgcgtggc gcaaaatact tcgatgtcgg ctcggccgca
     7261 cttttccaat tccgcccgca ccgttccggc cgcgagctcc ggcgaattgc aatcgcggct
     7321 aagatgtccg agcacgacgc gcgcgacctt gccgggcaat aattgcgcaa tggcggcggc
     7381 ggcggccgtg ttggagaggt ggccatgccg cgactggatt cgctgcttca ccggccaggg
     7441 ccgatgcgga tcgttctgca aaagcttttc atcgtgattc gtctcgatga ccagcgtttg
     7501 cacctggcgc aggcgctcaa tcgtgaggcg cgtggcatga ccgaggtcgg tgatgtagcc
     7561 gagcgcgctc gtccccgcgt ggaaagtata gccgaccggc tcaaccgcat catgcgggac
     7621 ggagaaattt tcgatcgtga cgtcgcagat cgaaaaactc gaaccggtgc gaaaaattcg
     7681 ccagttgcga tgcgcgccta atcccgcgga ggcggggtaa cgaatcgctt ccgcggtcag
     7741 cgcattgcaa taaatcggaa cctcgaattt gcggcagagc acctccagcc cgcaaacgtg
     7801 gtcgccgtgc tcgtgtgtca ggagcacgcc gtcgatgtcg ttaggcgtaa atccgcagcg
     7861 cgcgagccgc aggacgagct ggcgcgcgct caacccgccg tcgacgagca gccggcaatg
     7921 gctggtcgcg acgagtgcgc tgttccccgc gctcccgctc ccgagcatcg tcagactgaa
     7981 catctgaaga taattagcga agcggagcct tgggcacaaa cggaaaccct ttgcctcacc
     8041 gccggcgcga acaattttcg acagaggcgc gagtgtcgga tacggtttcc cggcaatgga
     8101 tgcgatcgat ctggccggtc tccggcgtga gtacgaaacc cagggactgc gccgggcgga
     8161 gctgcatcac gatccgatcg agcagttcgc gacctggttt tcgaccgcgc tcaattccgc
     8221 gctcccggac gcgaatgcga tcagcctcgc caccgccacg cctggtggaa aaccatcggt
     8281 gcgtgtcgtt ctcctgaaag gattcgatca gcgcggtttc gtttttttta ccaactatga
     8341 aagcgacaag ggccgcgagc tcgcctccaa tccgcaggcg gccttcgtcg tttactgggt
     8401 gcagctggag cgacaaatcc gcgtcgccgg ccgcgtcgaa aagacttcgc gaaaggaatc
     8461 ggagacctat tttcacgcgc ggccgcgcgg cagccagctc ggcgcctggg tctcgcgcca
     8521 gagcgaagtg atcgacgcgc gccgcattct cgacgcccgt ctggcggaga tgactgagcg
     8581 ttttgcgggg agcaacgaga tcgagctccc gccgcattgg ggcggctacc ggatcgtgcc
     8641 cgagacgatg gaattctggc agggtcgcgc gaaccggctg catgatcgat ttcgctacac
     8701 ccgacaaaag gatgcctcgt ggctgatcga gcggctcgcg ccatgattcg ttaggcagcc
     8761 gtcttctggc atggagctac ccgacgaaac aactcgttcg cgccgctgaa cgccggcggc
     8821 cgggccgcgt tactggtggc accctctgcc ggggattgcc ggggatggat tttgttccgc
     8881 cggtttattc cgacctgagc aaggcggagt ggaaaatcat gagggcctgg tatgacgaga
     8941 ccgatcggag cggagtgatt ggcgaatgcg catgagtatg aagcggccgt gcgggactcg
     9001 atctttggta tccagcactg caaatgggcg gcgtcttgtt gttgcacgat atgagccggt
     9061 tcgcgcaagc attcgatgtt acggcccaag gcggagtgcg ccgcgcgtgc gacgagtggc
     9121 gggagggaaa tccgcaagcg gaagcgatct gcctcaacgg cgagtcgcgc acgatggaat
     9181 tgcccagccc ggtttacaag gatgcttgcg ggctggggat cctccacaaa agcggagtcc
     9241 cggcgccgcc caacggcgac accgggaccg cagtctcact cacaaatcag aaacatatcg
     9301 aaaccccgcc gtagccatta agcctctgcg cgagctcgat ctgacggctg acgatctctg
     9361 cacctaacga ttgcgggtcg aagctggcgc ctccgcttcg ttcccctcgt gaccaccgaa
     9421 gaggtaggag aaaaccgcga agacttgcgc acgcggggca tcggtattaa cgccgaaaag
     9481 cggagaaaca tccagccgcg tatttttcgt cggtttccac gcgaccgtcg ggccgatgat
     9541 gaaacgagta gattcgcccg tcgcaggatc ttcgcgaata cctttgtcgc taaacacggt
     9601 tagctgcatc tccgcgccta ctttgagccg ctctttcggg agcaagaccg gaatcatcgc
     9661 gctttgggca aagccgtatt cgcggccgcg atcgccaccg acttcctgct cgtagaaaac
     9721 attcagtgcc cactccagtt tctcagcgaa ttcttccgaa aacagcaccc gcccttccac
     9781 cgcgtcgggc agcggaaggt gcatgtccag aaaatcccgt ccctcgcccg ggccaggttt
     9841 tgccggcgga ccttcatcat gcaaaatgtc accgatgccg aatttgtatt cgacgaacaa
     9901 ggttgggttg aaaggaatct tatcccaatc ggcaagggca taacgcgcct ccacgctgaa
     9961 acttctctcc tgggtcgttc cgcgataggt ctcgactgcg ttctcgaagg cgacgccgaa
    10021 acgatagggc aaaccaatct caacctcttc ggtgaaattg tgatcgggtc gatcgaactt
    10081 gagcgcatcg ccttcataga tgaaacctgc gtagacggcg cccggcggga gcacataggc
    10141 attgaccagg ttcgaaaacc ggctgcgcga gagggcgggc ggcgcgccgt atgcgctcgg
    10201 cacttcctcg cctctgatca ccatagtctc ctgttcaggg actgccgcgg tttgattagt
    10261 ggaggcgctc gctgggctcg cggcactggc cggtttcttg gcagtggagg tcgagttgct
    10321 ggtggtcgtt gccaccgtga gaggttcctt attcgaggtg accgcatcct gagcgagcag
    10381 ggtgttcgcg ggataaatcg cgccgaggca ggaagcggcc cacaggccga gcgtgaagag
    10441 tatcctgggg cgcggaacga cggatcgggc gaaacttttc tgtagcatgc gtgccgtgta
    10501 gctgaaggca gtcgggaagg caacaataaa atgataattt agtcatcttt ttccaaaacc
    10561 cccctttcgg ccgcagctaa tcgctcgctc cattcaccag cgccgggctc ggaaaccgga
    10621 gcgtaaccac cgtcccgcga ccaagctcgc tcaccacgga aagggagccg ccatgcaagt
    10681 cggcgatcga tttcaccaat gccagcccga ggcccgtgcc ttcagagctg cgcgaccggt
    10741 cgacccgata aaaccgatca aagacgcgcg ggatatgttc cgccgcgatc ccgcaaccgc
    10801 tgtctcgcac cacgatttgg gcgccatccg tcccgaccgc gagtgccatg acgatctggc
    10861 cgccgttagg cgtgaagcgc acggcgttat ccaccaggtt gctcaccgcg cgtccgaaca
    10921 aaactgggtc cgcgtaaacc tcaccttcgc cttcacaccg gatgacgagg cggcgttcct
    10981 cggcgatcgc ctcattgtaa gcggcgattt tttcgatcgc ggcgcggccg tcgaaaagcg
    11041 agcactgcgc ctgccgctcg gctgcttccg cgcgggcgag aaagagcagg ttgtcgatga
    11101 tcccggagag ccgcgcgcac tcggccacgc tcgattcgat tacggcctga tactcgtcag
    11161 gcgaacgcgg ccgggccagg gccacttccg cttcgccgcg aatgttggcc agcggcgtgc
    11221 gcaattcatg cgcgagatct gccgaaaatt gcgagagccg cgtaaacgaa tcctcgagac
    11281 ggtccagcat ctcgtcgaac gcgatcgcga ccggctgcag ctcgcggggc cactccgccg
    11341 gcgggacgcg ttcgtgcaac tgatgcgggc cgacgcgctg gagcgacctg gtcatggcgg
    11401 ccagcggctg caaacctcgt ttcgtcacgg tgatggcgat gacgacggaa gcaaaggtcc
    11461 cgcaagcgag cacggccgcg accaggaagc cgaactccag catgaaacct tcatcttccg
    11521 agcgatcctg ggcgacctga atcgtgtatg gttgaccgtg caccaccgcc gcggcggaga
    11581 ctaacgagaa taacttgcca gctgtccgga agtcgaacgg gccattttcc gacacgccgg
    11641 cgttccggcc gcgggtaaaa agttccggtg gcagcaagcg tttcatcccg ggcgtctcgg
    11701 ccacggtggc tccggccgaa tcgaccactc gcacccagta ggccgcccgt tctccgccac
    11761 ggagcgtttt caattcgttc tccagaaggc gcggctcccc ggcggttgtc agctccgcgc
    11821 ggatggcaag gaccttgtcc acgaggacgg cccggtcctc ttcgaaagcg tgccggacga
    11881 caatccaata gaggaccgca agcccgcagc agaggagcag ggccgccgcc ggggtaaaga
    11941 ggaagaccag ctgcgaagca atcgaccgcg gctcagtcga ctttgaggac ataaccggcg
    12001 ccgcgaaccg tgtggatcaa ctttctcgaa taaggatcgt ccactttggc gcgcaaccgt
    12061 cggaccatga catcgaggac attggtgccg gtttgaaaat caatccccca gacatggctc
    12121 tcgatgtcct tgcgcgttac gacctcgccc gccgagcgaa ccagttgtgc gaggagcaga
    12181 aattccttgg cggtcagatg aagcggatcg cccgcccgga ccgcccgatt gcgtcgcgta
    12241 tcgagttcca aatcctcgat ccgcaactgt tcaccctgct gcgccgggct ccggcgcagg
    12301 agcgagcgca cgcgggcgac cagctcggag aacgcgaatg gcttgactag gtaatcatcc
    12361 gcgcccagct cgagtccctt gacccgatcc ggcacgctgt cgcgcgcggt gagaaacaaa
    12421 atgggcgtgt gcgctccgcc gctgcgcaat tcctcgacga cggcccagcc gtccttcttt
    12481 ggcagcatca catcaaccag gagaagatcg aaacgcgtcg cgagtgcgag ttggagtccg
    12541 gtctctccat cgcttgcgga ctcgacggaa tagccttcct cctcgagacc cttggcaata
    12601 aacgcggcgg tctttttctc gtcctcgatc aacaggatgt gcattgaaga aggtataaag
    12661 gttgccttcg ccgcgttcaa gcttggcgga gcgccgcctc ttactaccgg gcggcgcgtt
    12721 tcccgtcact cgaaaaaccg ctccgcgtgc gggccgcaag tcgagctcca gcgtccaggc
    12781 gctgcggggt tgacgggcga gatgcctaga ggtctccgcc atgcggcccg gatcgaggaa
    12841 cgtttttccc ttcgccggcg gcgatcatgt cgcgcgccgt ctcccgcgca cggacgaaac
    12901 cactcccgtg gcgctgcgcc acgacgtccc agctccgcac gcactcgatc atcttgtttt
    12961 tcgttaggct attttggggg ggccgctggc ggcaggataa acccacgcgg gcgccgctcg
    13021 caaagcgatg gtcgaaaact gatctcgcaa tagcggcgcg agttgctaga acctgtgcca
    13081 ccgactttga aaaattatct cagactgaat ctgagcctgc tttactggat cttcgtcgct
    13141 ccctttcggg gagagatgtt cgagctcgcg ccggtttttc gccagatggt ggcgatcggg
    13201 gtgcgcgccg ctccgatggt cgcgctcacg gcctgcagcg tgggcgtaac cctcgcgatg
    13261 cagggcgcac attctctcca gcaactcggc gcggaaattt acgtgccgga cctcgtcatg
    13321 gtcaccttgc tgcgcgagat gggcccggtc ctgatcgccg tcatcgtgat cggacgcagc
    13381 tgttcggcgg tcgccgcgga attgggcacg atgaaagtgt ccgaggaaat cgaggccctc
    13441 gaagtgatgg cgatcaatcc gatcagttat ctgatcgtgc cgcgctttct ggcgatgctc
    13501 gtgatgctcc cggcgctcac catcctgggc gattacatcg gcgtcttcgg tggttgggcg
    13561 gtctgtcggc tcgcactcga cttcgatacc gccggctacg tcttgcgcgc cgtgcaaagc
    13621 gccgacacct gggatcttta ctccggcatg atcaagagcg tcgtctttgc ctggatcgtc
    13681 atcacgatcg cctgtaacgc cgggttgcac gtggagggtg gcgccgaagg cgtcgggcaa
    13741 gccaccactt cctccgtcgt gcaggcgctc ctcgccatgc tcgtgacaaa cgccatcctg
    13801 acttcgatct ttttctttgg cgcatgactc cggaaaacca gccagtcgtc gaagtgaaga
    13861 atctcatccg aaaattcggc gagcgcagtg taatcgacga tctcaatttc cagatcgatc
    13921 gcggcgagac cctcgtcatc atgggcggga gcggttgcgg caagagcact ttgctccgcc
    13981 acatcgtggg cgtgatgaag cccaactccg gctcggtcca gattttcggc gaagagatca
    14041 ccacgatgaa cgaccgcgag atcgccagag tgcggcgacg cttcggcatg ttgtttcaat
    14101 ccggcgcgct cctcgcctcg ctcaccgtcg gggagaatgt cgcgctcccg ttgcttcaac
    14161 acaccgacct cagccccgcc gaaatcgacg aagtcgtgac ccgaaaactg caaatggtcg
    14221 gcctgaccgg tttcgagaat ctcaagccgg ccgagatcag tggcggaatg aaaaaacgcg
    14281 tcgggctggc ccgcgccctg gcactcgaac ccgagttgct cttcagcgat gaacccactt
    14341 ccgggctcga tccgatcatg accgcggtgg tggaccggct gaccctcgaa ttgacccgcg
    14401 gcagcggcat gaccgcggtg gtcgtgaccc acgacatgac gagcgccttt cgcattgcga
    14461 cccgcatgat catgctcaac cacggaaaga tcatcgcgca aggcacgcct aacgagatcc
    14521 gggcaaatcc gaatcccgac gtccagcaat tcatcaacgg cgaagccgat ggccctgtgc
    14581 cgatgagtct ctcccagagc gagcatcaga attccgctca cgtccagccg cgtccgctcg
    14641 tcggcgcgcg cggccgcttc catcgcaaaa catcatgaaa cgaaattttt cagattatct
    14701 cgttgccctc gcggtcgtcg cttgcagcct cgtcctgctc ggggcgctga cctttgctct
    14761 ctccggttat cgattgcaga aaccgtcccg cgttctgcag atcgactacg aggatgtcac
    14821 cgggatcaag gtccattcag aagttcgtta tgcgggagcg cccgccgggc gggtgattgc
    14881 gatgcgtcat ttgagcgccg cccagcgcgt ggccgagccg aacaagaagg acgcggtccg
    14941 ggtgagcatc gaactcctcg acagtgttcc gcctttgcca tccgacatcg cggcctctct
    15001 gagctccgac tcgatgctgt cgccgaagtt tgtctcactt tccgccggga cgccggggaa
    15061 ggcggtcttg cctaacaatg ccataattga aggcgcgccc gcctacggca ttgaccagat
    15121 catcggcgcg gtcggaccgg tcctgacgaa cgcgaacaag ttgatcgaca atctgaatgg
    15181 cacggcaacg aacttgaacg gcacggtgac catcgtgaaa acggatctcg gcctgtttct
    15241 tcccaagctt tcaccggttg ccgacacggc gaaagtcgac ctcgaggagc tgcaaaagac
    15301 gattcagggt ttaaacggcg tggaagccaa ggcgagcaat gttttcgcga ccgctgatac
    15361 gttcgtcggc tcgaccgacc aacaactgcg cgagcagatg cgcgagctgc acgtgattct
    15421 gttgaacctc aaggtcatta ccacgcatgc caaggcgctg gtcgataccc tggcggagaa
    15481 accgaaccgg atcattttca gcggcaaagc caacaaactc acgcccgaag accagatcat
    15541 caaaagccac caggcgttgc cggccagaac accgtaatcg gcctaacgaa tccccatttc
    15601 ggatcgcgct cgcgccgcgg gcgaaatcgc gttagggagt tggatggtaa tcttccagcg
    15661 cacggccgtc gggtttgctt tccttgtttg gttcgcggag ctcgtgcctc cggcgcgggc
    15721 ccaggaatcg gaaacggcgc cgctcgtttc gcccccggcg gccgtggcca cgccgacctc
    15781 ttctcctgtt ccttctgcga ccgccgctcc ctcgccgtcg ccggtgcagt tgaccgatat
    15841 tcttgccaaa ggcgtcaagg cacgcgtgat cgggccggcg gtgatgggcg gccgcgtctc
    15901 ggatatcgcg atcgatccgc gtaatcccgc cgtgttctat gtcgggctgg gaacgggcgg
    15961 ggtcttcaag acgggcaaca atggcgtcag tttcgatccg attttcgaca agcaaccggt
    16021 gctctcgatc ggcgcggtgg cgatcgcccc gtccgactcg gatgtcgtct gggtcgggac
    16081 cggcgaagcc aacgatcgca attcgtccga gtggggcgac ggcgtttatc gttcgaccga
    16141 tggcggcggg gcatggacga acgttggctt gaaggaaagt cgcgccatcg cgcggatcgt
    16201 ggttcatccg acgaaaccgg aggtggccta cgtggcggcg atgggaaatc tttggaaaga
    16261 cggcggcgag cgcggtctct acaaaacgac cgatgccggc aagacctgga aacttgttct
    16321 gcacgcgccc gtgcctaacg acgcgcgctc cggttgcggc gatgtcgcgc tcgatccgtc
    16381 aaatcccgag atcgtttacg cggcgctcta cgcccggcaa cgcactccgt ggtcgtttgc
    16441 ctatggcgtg acggcgacga acgggttcga cgtcggtgga attttcagga gcagcgatgg
    16501 cggcgcgagc tggaaaaaat gcgccggcgg tctgcctggt ctgacgggcc gcatcggcct
    16561 cgccgtgtcg gcgagcaaac caaaactggt gctggcgatc gtgcagagcg acgagggcgg
    16621 tgcgagcgat atcagtgatc ttcacagccg gcggggcggc gtttttcgct ccgaagacgg
    16681 cggcgggaaa tggacccgga tgagcgacat caatccacgg tccttttatt tcagccagat
    16741 ccaggtcgat ccgtccaatg atcaacgcgt ttatgtgctc ggcatggcgg tgctcgtgtc
    16801 cgacgacggc gggaagaatt tccgggaaga tctttcggag aagttgcatc ccgattgtca
    16861 tgcgctcgcc attcaaccgg gcacggcccc gtcgcccaag cgggcgaaac ccgaggacaa
    16921 gaacaagccg ccgaaggcac cggtctgcca gcgtctcctg ctcggaaccg atggcgggct
    16981 ttatcaaagc tacgccgcgg gcaaagcctg ggatcacctc aatcgatttc cggcgggcga
    17041 attttaccgc atctcgctcg atgacacgca gccgtttttc cggatcgcag gcggtttgca
    17101 ggacaacgag agttttgtcg gaccgagcca ggtgccaagc aaggaaggca tccggaattc
    17161 cgattggacc gcgctcgaag gcggcgacgg gttttacgtc gttttcgatc cgtcggatcg
    17221 cgacattttc tacgccgaga gtcaggaggg ctacattcat cggattaatc tgcgcaccgg
    17281 cgagaagcgc gacctccgcc cggaggcggc ggaaggccag gagcgttatc gtttccactg
    17341 gaacgcgccg ctgatcggga gccgggatca acccggcgtg ctttacctcg ccggcaaccg
    17401 cgtttttcgc ctcacggatc gagcggaaca tttccaggtg atcagtcccg acctcacgca
    17461 ccacgatccg gcgcgggtca ataccagcgg cagcggggcg gagaattacg gcgtcgtcta
    17521 ttcgttagcc gaatcaccgg ccaaagccgg tttgctgtgg gccgggacgg atgatggacg
    17581 gctttggttg acccaaaacg acggcgcgaa ctgggcggag ctgacttcga agctgccgga
    17641 accggcgcgt ggccaatgga tcgtgcgcat cgagccgggc gcgaaggatc caaaggttgc
    17701 ctacgtggtg acgaacgcct atcggcgcgg cgacgatcgg ccatttattt tgcggaccgc
    17761 ggatctcggc caaacctggc agagcgtgat cggccagggg cttctaccta acgacccggt
    17821 ggaggtcgtg cgggaagatc cggtgaactc caatttgctc tatgccggga cgcactttgg
    17881 cttgttcgcc tccttcgacg gcggcgggca ttggctgcgg gtgggcgatt tgcctaacgt
    17941 gcgggtggac gatctccaga ttcatccgcg cagcgccgat ctcgtcattg cgacgcatgg
    18001 ccggagtctc gagattttgg acgacacacg ccctttccgt gagctaacgc cggaagtgat
    18061 ggcgcaactg gcgcatcttt tcacgatcgc gcccgcgcgg ggattttacc tgctcgcggg
    18121 ttcggcggat tggaacggga aaggcattta tcgcggagaa aatccgccgg agggcgcctt
    18181 gatcaccttt tgggtgaaag agttcaccgg cgatgaggtg aagattgcga tcaccaactc
    18241 cgcggggcaa ccggtggcga acctgaaagc cgcgggtgcg ccggggttgg gtcgggtcag
    18301 ctgggatctg cgtccaacgg aggatgtccg gatcaagtac ggtggcgacg atccaaaaaa
    18361 atttgtggcg agcgacgatt acacagcgga gctgagctat ggcaaagaaa agatgaaaca
    18421 gacctttcac gtcgagatcg cggaaggaat tgttactcgt taggctgggg ggttgtcctt
    18481 tcgtcaggca ccagtctccg ttggatgtct caggccggga cgaccgtgat gcgcgaaaaa
    18541 accattgccg gggtcggggg cgcgctcctc ctggtcgcgc tgctcgcggc ctacgcaaac
    18601 cattttcaca atggcttcca tatggacgac gggcacgcga tcgtggacaa cgtctccatt
    18661 cgcgcgctgc gaaacattcc gctcttcttc cgtgacgcga cgaccttcag cgcgctcccg
    18721 agcaatcaat catatcgccc gctcgtttcg accctgctcg ccatcgacta ccgacttggc
    18781 cacggactcc agccattttg gtttcatctt tcaaccttca tccttttcct cgcgctcacg
    18841 ctgctcctcg ccttcgtcat tcatcatctc ctcgccctcg gcgcagcctc atcgccgtgc
    18901 ccttggattg cgctcgcccc cgccgcctat tacgcgctga accccgccaa cgctgatact
    18961 gtcaactatc tcattgcctc gtcggaggtg atctccgcgc tcggcgtgat cgcgtcgttt
    19021 gcggtctatt tcgccttccc gcggtgccgc caatattttc tttatgttct cccggccgcg
    19081 atcgccatcc tggcaaaacc aaccgcagcc atcttccccg tgctctttgc ggtgtttcgt
    19141 ttgctctttc ggggccccgc agtcgcgggg cccagacccc gcccgcggtc gcggctcgcc
    19201 ttggaaattg tcccgccctt tgtgatttgc ggagcgatgc tctggttcgt ccaggacatg
    19261 acgccgcgaa actgggtcgc cggcgcggcg aatgcgcaca actatctgat cacgcagccc
    19321 tacgtggccc ttctctattg caaaaccttc ttctggccga ccgggctcaa cgccgactac
    19381 gacttgaatc cgtttgtgac gacgggcgat gtgcgtttgt ggatcggttt cgtgttcgtc
    19441 gttttgctga gcgcggcggc gatcgtggct gccgttggca agaggacgcg gttgatcggc
    19501 tttggtctcc tctggttcct gctcgcgctg ctgccgacgt cgctctttcc cctcgccgaa
    19561 gcgatgaacg atcaccgggc attcctcgcc tacatcggac tcgtgattgc gctcgccggc
    19621 gccgcggcgt tgctcgtcgc ccgcctggat cgaccgcggc gttggacgaa aattgccgcc
    19681 gggtgcgcgg tcgcgctctt cttgtgcgct aacgcctgcg ggacattcca aaggaacgag
    19741 gtttggaaaa ccgaggaaac actctggcac gacgtggtct taaagagccc gcgcaatgga
    19801 cgcggtttga tgacctatgg catcacgttg gtgaacaacg gcaattttgc cggcgcgctc
    19861 gattatcttc gccgcgcaca accgctcact ccgccgtatt ccgtgctgtt gatcaatctc
    19921 gcgatggcgg aaaacgcgac gggagagatc gcgaccgccg aacagcattt taaggacgcg
    19981 ctgcgactgg caccattgtc tcccgacagc tacatttatt atgcgcgcta cttgctcgcg
    20041 cacgcacgcg ttgacgaggc gcagacgctc ctgcgacggg cgttggagtt gagtccgacc
    20101 aatctgaccg cgcgcgaact tttgcagaaa gcggggacgc acgaggtgga ggcgcacgcc
    20161 accgagggcg acgcgctcct gcgtcagggc aggttcggcg aagcgattac ccagtatgaa
    20221 gcggtcttgg aaatcgcgcc cgatttcgtg gcaacgctga acaatttcgc ctggctctta
    20281 gcgacttgtc ctgatgggtc gctgcgggac ggcgcccggg cccttgagct ggcaaagaaa
    20341 gccgatcggc ttgccggcgg gggcagcccg gttttcctcc ggactctggc cgccgcctgc
    20401 gccgaaaacg gccgtttcaa ggaggccgtc gaaaccgcgc agcgcgccgc gcaaatcgcg
    20461 cttgcccaga aaaacttcgt cctggcaaag aagctggaaa aagacgtcga actctatcga
    20521 aacaatcttc ctctgcagcg atgacctccg gctcggcccg cgatcgccgc cggcattgtc
    20581 actcgttagg cagagggaaa atctggccgt cgacgcgact caatcctcgg agtccgcgtc
    20641 cctgtcttcc tcgatctgct cgttctccgt cgaaaagcgg cgtttgcgtt tgttcgccgg
    20701 caacggcgaa agtgtcatcg tgatgttccg gccggcggtc ttcggctcca tttccacctg
    20761 cgccatggtc gcgagatcgt cgcgcacctt gaacatcaac tgcatcccga actcctggtg
    20821 cgccatctcc cgtccgcgga attgcagctg cacccgcacc ttgttgccct tgtcgaggaa
    20881 ctgctcggcg tggcggattt tcgtcaggta atcgtggtcg tcgatgttga cccgaaactt
    20941 gatctctttc agcttcgtac ccgacgtctt cttgtctttt tcctgcttcg agatgtcgta
    21001 acggaatttt ccgaaatcga cgattttgca gacgggcggg ttcgcggtcg gcgcgacttc
    21061 caccaggtcg aggcccatgc cctgggcctt cttgatcgcc tcggacaact tcatgacgcc
    21121 gagttgctcg ctgctcgagg cgaggatgac gcggacttcg cgggcgcgaa tccggccgtt
    21181 aacacgaatt tgttgttgca gaattaatta tctccggccg gcgacgaggg agtgagactg
    21241 ccgccgtcat tcttattctc gcaatctccc gtcgcgctcc ccagggagcg cgccgcactc
    21301 ggaaaccttc gcgcctgcga ggagcgcgtc gattggttcg cgacctgacc cgaaccgagg
    21361 ggaagttgta tccgacatcg ggaaagtcgc aaggcgtttc tgagcggagg gaaatccacc
    21421 gcctgcggct gaaagacgca ccggatcaag cgggtggatc gccgaaatgc gccgcgtgcg
    21481 cgcgatagcc aacgatgtcg tccagcatcg tgtcgagcgt cgccgccagc ggcgcggctt
    21541 tggcgaagcc ggaaaagatc gcccgatcgc cggtcggagt gtcgatcaca aacgtgggcc
    21601 gttgcgtcac ctggagcgcg tgaaattccg cggtgctctg gcgcacgcgc gcttcgatcg
    21661 cgggcgtttt cgcgtgctcg agcagatgct ccggttccag tccggcggct tcggcgccga
    21721 cctccgcggc gacgtcccaa tgagtaaaac gtttcccctg acgcaatccg gcggtcgaga
    21781 tggcgatcca gacacgatcg tccacgacgc caaaatcgcg cgccgcctcg gccacgcagt
    21841 tcggagccag atattccgcg ctgtccggct taacccagcc ggcgtgcaac atctgcgggg
    21901 aacgcatcaa cattccgctg cgccggtagt accattccgt gtgcgcgcgc gtcgggggca
    21961 gccccttctt gtccatcagc gcgaccttcc aatggaaatc gacccgatcg ccatattttt
    22021 cgcgcagttc actccaggct gggatcgccc agtgacacca ggaagaaatg acatcgaggt
    22081 agttggtgat ggtgagtcgc ataagaccta tatgacctat cacggcgcat gcggaaaaga
    22141 acaaatcgcg ctcgattgac aaactaccgg cctataaccg atttcctctg cgcccagcgc
    22201 gacgaaatat tttggtcgac ctcaatcaca ttctgctttt cgtcgcggtc gtctcaccgc
    22261 tgattctgct cgcgcgcatt gcgcagctgc gcaacccgcg gaacgccggc tggcggattg
    22321 ccgcggtcat agtgctcgcc gcttgcgccc tggcctggtg gttcctgccc tcgattgccg
    22381 gtttcgtcgg cggcacgttc tggtgtttgc tgctcctcat cccctcggtg gcggagcgga
    22441 agatcgacga cctgctcgtc gcgcagcgtt tcgcgcgcgc ccgccagctg gctgtcgtga
    22501 ggcgaattct ccatccctgg gaggactcgc cccaacgccc cgctttgctc gactgtctcg
    22561 cgctggcgag cagtggccgc ctcgatcgcg cgctcgatca gctcgccacc gaacgcgccg
    22621 cacccacccc ggccggatcc ttcgcgagcg cgctcacctt tgcgctgacg gagaactggg
    22681 cgggcctcgt gcaatggtgc cgacgcgact tgtcggtgac ggcgaatccc gccgtgcttt
    22741 cgctttattt ccgcgcgctc ggcgaaaccg gcgcgctgga ggagctggtc cgccagtttg
    22801 cctcgcgccg cgcgagccgc gaaccggagc tgacgcctga cgccctcccg cgctttaact
    22861 tcgcgctcgt cctggccttc gccgggcgga cgaacgatct cgttaggcta ttcgccggcg
    22921 acctccggca aatgccgctc gagcggcggc agttctggct cgcgacggcc gagctggcgg
    22981 ccggggatgg ccgcgccgcg cgcgagcgtc tggagaagtt gcgcgcggag acgcgggacg
    23041 cggttctgcg gcgttcggtc gatcgtcgcc ttgggcaggc cccggagttt ccgccctttt
    23101 ccgccgcggg cgcagggctt ctcgcgcggc tcgtggtcga gacgatgggg acgcagcaga
    23161 ctgggacgcg cccgcggacg agcggcacgc cggcggtctg gggattaatc ctcttgaacg
    23221 tcgccatgtt tggtgccgag tggatcttcg gcggcacgac caacacgcgg acgctgcaca
    23281 atctcggcgc gctcgagccc gacgcggtga tcaggcagca tgagtactgg cgtctgctga
    23341 ccgcgctctt cctgcactac ggcgctctcc atatttcgtt taatctctac gccctttacc
    23401 tgctcggtcc ggaactggaa cgaatgattg gttcgatcaa attcacaatc agctatctgc
    23461 tcgccggact cggttcgagc gccggggtgg ttcttttgcg ggcgctcgac ctgacgaatg
    23521 ccaatctgct cgtcggcgct tccggctgtg tcatgggcgt gatcggcatt tcggccgggt
    23581 tgcttttgcg ccatcggcaa tctccactcg ccggccgccg cctgcgcaac attgtggtca
    23641 tcgtggtctt gcaaacggcg ttcgatctct ccacgccaca ggttagcctc gcggcgcact
    23701 tgagcgggtt cgtcaccggc gttgcgatcg ggatgattct cgctcggcag cgccgtcgtg
    23761 ccgttccttt ctagccggca ggtcgccagc ccgggaaacg gaacgcttgc tgctactcat
    23821 tttgctgtca gagtttcgcc tctgtcttaa aggccgcgct tctccgtcat gccgaacatt
    23881 cagacctaca acgtcattcc caatctcccg gcgcagctcg agccgctgcg cgaaatggtc
    23941 ttcaatcttt ggtggacctg ggagccatcg gcccgccggc ttttccggca tctcgatccc
    24001 gggctgtggg agcgcaccaa tcacaatcct gtccggatgc tccagctctc gcgccaggcg
    24061 cgcctcctcg aagtggcgaa ggacgaagtt ttcctgcgcg agctcgcgga agtgcacggc
    24121 gcctataaga aatatctcgc ccgcaaagac acctacggaa aaaagggcgc ggggaacgag
    24181 atcaagaaac cgatcgctta cttctccgcg gagtttggct tccacgagtc gatcccgaat
    24241 tactc
//
LOCUS       DATMNS010000110         3421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_158875, whole genome shotgun sequence.
ACCESSION   DATMNS010000110 DATMNS010000000
VERSION     DATMNS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3421
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158875"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1585
                     /locus_tag="VNW28_03980"
     CDS             107..1585
                     /locus_tag="VNW28_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HXA09114.1"
                     /translation="MYSRAIPVRTEPTQIQNFIDGQFVPPIGGQYLDNIEPATGKPYS
                     LVPDSDSRDVEAAVAAAEKAFPAWSRTSVAERSRILLRIADLIDRDLEKFARAESVDT
                     GKPLSLARSLDIPRASSNFRFFATAILHTENEAHVTDGVAFNYTLRQPRGVVGLISPW
                     NLPLYLLSWKIAPAIAVGNTAVAKPSELTPMTAFLLGEVCREAGLPNGVLNIVQGTGP
                     NVGSVITAHPKIGTISFTGGTVTGRKVAEAGAPLFKKVSLELGGKNPNIIFADADLEA
                     AIAGSVRSSFANQGQVCLCGSRVFVERPAYDEFVRRFTEKVARLQQGDPLDEKTEQGA
                     IVSQMQLEKVKFYVDLATQEGGQIALGGAAPSPPNERCRDGYFFPPTVITGLPVGCRV
                     NREEIFGPVVTITPFDNEAEVIGYANDTEYGLASSVWTQNLSRAHRVAEQIHTGTVWV
                     NCWLVRDLRVPFGGMKQSGVGREGGEEALRFFTEAKNVCIAK"
     gene            1792..2103
                     /locus_tag="VNW28_03985"
     CDS             1792..2103
                     /locus_tag="VNW28_03985"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HXA09115.1"
                     /translation="MTTADLPIAINRESIARFCGERGIRSLSLFGSVLRDDFDPKRSD
                     VDVLAEFEPGALRGVGLGYFTFAEELGAILGHKVDFCSQLHPSLGERVRREAVKVYEQ
                     S"
     gene            2090..2440
                     /locus_tag="VNW28_03990"
     CDS             2090..2440
                     /locus_tag="VNW28_03990"
                     /inference="COORDINATES: protein motif:HMM:NF014043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HXA09116.1"
                     /translation="MSSRDPTLTLRQIVEFADEVAVLVATRARENLESNREFRRALER
                     CVELIGEAATRLPEDWRAAHPKIPWRQIIAPRNILIHGYDVVQAEVFWNVATNDVPNL
                     RDAIIALASEGSDQ"
     gene            2437..2862
                     /locus_tag="VNW28_03995"
     CDS             2437..2862
                     /locus_tag="VNW28_03995"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXA09117.1"
                     /translation="MSESFESQRAAEPVGAFPHAKRVGDLLFLSGIGPRVRGSKEIPG
                     VNFDAERNIVSYDIEKQCHAVFENVRLVLEDAGAKWSDIVDVTVFLTNMKKDFPIFNR
                     IYPEYFAGPGKPNPTRTTVEIGALPTPIAIELKVIAAMQ"
     gene            complement(2953..>3421)
                     /locus_tag="VNW28_04000"
     CDS             complement(2953..>3421)
                     /locus_tag="VNW28_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09118.1"
                     /translation="FWANHLGVTHLMIYGLSVHLYAIMMIVFAARDLYLIKRFDYAAP
                     VLILQKQIAGLREWHLRAGVWFGVAGCFIWIPLLLMIFQGLGADVWRRNPGVVGWLFV
                     SGLICLGVFYGIIAWSRRPGREKFARSLEDSSAGRSVQRAQSVLAELAQFERE"
BASE COUNT          684 a         1016 c         1033 g          688 t
ORIGIN      
        1 gcacgtcctg ccgcgaaatc gccatcccgg gcggaagctt tctcggaaat tccggacgtg
       61 cggaagcacg tcgcttcgaa ggcgtttggc gacgtcggag agccgcatgt atagtcgcgc
      121 cattccagtg cgcacggaac cgacccagat tcaaaacttc atcgacggcc agttcgttcc
      181 cccgatcggc ggccagtatc tggataacat cgagcctgcg accgggaaac cctattcgtt
      241 agtcccggat agcgatagcc gcgatgtgga agccgcagtg gccgcggcgg agaaggcttt
      301 tcctgcctgg tcgcgcactt cggtggcgga gcgttcccgc attctcctgc gtatcgccga
      361 cctgatcgat cgcgacctcg agaaattcgc gcgggccgaa tctgtcgata ccggcaaacc
      421 tctctcgctc gcgcgcagcc tcgatattcc gcgcgcttcg agcaatttcc ggtttttcgc
      481 gacggccatt ctgcacacgg agaacgaggc gcacgtgacg gatggcgtcg ctttcaacta
      541 cactctgcgc cagccgcgcg gcgtggttgg tctcatctcg ccctggaatt tgccgctcta
      601 tttattgagc tggaaaatcg cgcccgccat cgcggtcggg aacaccgccg tggcgaaacc
      661 gagcgaactg acgccgatga ccgcgttttt gctcggcgaa gtttgtcgcg aagcgggctt
      721 gcctaacggc gtcctcaaca tcgtgcaggg aactgggcct aacgttgggt ccgtcatcac
      781 ggcgcatccg aagatcggca ccatctcctt caccggcggc accgtgaccg gccgaaaagt
      841 cgcggaagcg ggtgcaccgc tttttaagaa ggtgtcgctc gaactcgggg gcaagaaccc
      901 gaatatcatt ttcgccgatg ccgatctcga ggccgcgatc gctggcagtg tgcgctcctc
      961 tttcgccaac cagggccagg tttgcctttg cggctcgcgc gttttcgtgg agcggccggc
     1021 ctacgacgaa ttcgtccggc gctttactga gaaagtggcg cggttgcaac agggcgatcc
     1081 gctggatgag aagaccgagc agggcgcgat cgtttcccaa atgcagctcg agaaagtgaa
     1141 gttttacgtc gacctcgcca cgcaggaggg cggccagatc gccctcggcg gcgccgcacc
     1201 gtctccgcct aacgagcgct gccgcgacgg ctatttcttc ccgccgactg tcatcaccgg
     1261 cctgccggtt ggttgccggg tcaatcgtga agagatcttc ggcccggtcg tgacgatcac
     1321 gccgttcgac aacgaggcgg aagtcatcgg ctacgccaac gacaccgaat acggcctcgc
     1381 ctcgagtgtc tggacgcaaa acctgagccg cgcccatcgg gtggcggaac agattcacac
     1441 cggcaccgtc tgggtaaact gctggctggt gcgcgatctg cgcgtccctt tcggggggat
     1501 gaaacaaagc ggcgtcggac gcgaaggcgg cgaagaggct ctgcgcttct ttacggaggc
     1561 gaagaacgtt tgtatagcga aataggttta tgccgtgaag cgctgcgcct gccccgccgt
     1621 agccttggcg caggcaggct ttttcaacga gcctaagaat gtctgcatcg caaaataacc
     1681 taggcgtgga agctccctca gcttgttaat tcccactgac gaagagtacg ataacgcgct
     1741 caccgattcg aataactggc cgttctctcc gcggatgtta acctctttct aatgacaacg
     1801 gctgacttgc caatcgctat caatcgtgag tcgatcgcgc gcttctgcgg cgagcggggc
     1861 attcgttcgc tctcgctttt tggttcggtt ttgcgcgacg atttcgatcc gaagcgaagt
     1921 gacgtggatg tgctcgcgga attcgagccc ggtgctctcc gcggcgtggg actgggctac
     1981 tttacgttcg ccgaggaact cggcgccatc ctcggtcaca aagtggattt ctgttcccaa
     2041 cttcaccctt cgttaggcga gcgcgttcgt cgggaggcag tcaaggttta tgagcagtcg
     2101 tgatccgacg ctcacgcttc gtcagattgt cgagttcgcg gatgaagtcg cggttctcgt
     2161 tgcgacacgc gcccgcgaga atttggagag caatcgtgaa tttcgtcgcg ctttggagcg
     2221 ttgcgtcgaa ttaatcggag aagcggcgac gcgtttgcct gaagattggc gggcagcgca
     2281 cccgaagatt ccctggcggc agatcatcgc gccgcgaaac atcttgatcc acggctatga
     2341 cgtcgtgcag gccgaggtct tctggaacgt cgcgacgaac gatgtcccta atctgcgaga
     2401 cgcaatcatt gcgctggcaa gcgagggttc cgaccaatga gcgaatcctt cgaaagccag
     2461 cgcgcggcgg agccagtcgg ggccttcccg catgccaaac gagtcggcga tcttctcttt
     2521 ctttccggca ttggaccgcg ggtgcgcggg agcaaagaaa tccccggcgt gaacttcgat
     2581 gccgagcgca acatcgtgag ttacgacatc gaaaagcagt gccacgcggt ttttgaaaac
     2641 gtgcgcctgg tcctcgaaga tgccggcgcg aagtggagcg acatcgtcga tgtcacggtt
     2701 tttttaacca acatgaagaa ggacttccca atctttaacc gtatttatcc ggaatacttt
     2761 gccgggcccg gaaaaccgaa tccgactcga acgacggttg agattggcgc cctgccgacc
     2821 ccgatcgcaa tcgagcttaa ggtgatcgcg gcgatgcagt aatgtgggag gggcctttgc
     2881 gcctcgatga gtcagcgcgt ggctttactg atcgggccaa aaaggcttct ccacattttg
     2941 cagagatcgc gtctattcgc gctcgaactg ggccaactct gcgagcaccg attgggcgcg
     3001 ttgcacactg cggccggcgg agctgtcttc caggctgcgc gcgaattttt cccggccggg
     3061 ccggcgtgac caggcgatga tcccgtagaa taccccgagg caaatcaatc cgctcacgaa
     3121 aagccagccc acgacgcccg gatttcgccg ccagacgtcc gccccaagac cctgaaagat
     3181 catgagcaga agtggaatcc agatgaaaca tcccgcgacg ccaaaccaca cgccggcgcg
     3241 caggtgccac tcgcgcagac cggcgatctg cttctggagt atgagcaccg gcgcggcgta
     3301 atcgaagcgc ttgatcaggt agagatcgcg ggccgcgaag acgatcatca tgatggcgta
     3361 aaggtgcacc gagaggccgt agatcatcag gtgcgtaacg ccgagatggt tggcccaaaa
     3421 a
//
LOCUS       DATMNS010000111         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_511308, whole genome shotgun sequence.
ACCESSION   DATMNS010000111 DATMNS010000000
VERSION     DATMNS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511308"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1164
                     /locus_tag="VNW28_04005"
     CDS             229..1164
                     /locus_tag="VNW28_04005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXA09119.1"
                     /translation="MPATVHASRDWRIPAAWLTLCVVWSSTWLVIKIGLRDLPPVSFV
                     AWRFVVAVLVLLAISIGRTHLLPRRGSDYLVLGLTGVLMFSVNYALLFWAELHVSSGL
                     AAVIQASIPIFGMIFAHWLLPEEPLRWQRLAGALVAIFGVGLICARLLSFNGWLAFLG
                     GLGITIGAASAAFSNVLLKARKLQLAPSMLAAWQMIFGIVPLLLIGLRLDGNPLLFHW
                     TGRAIFCLLYLAVVGSSFTFLLLYWLMPRMSVTNLQTISLITPPGAIALGWALGDERL
                     SAWSLLGAGFVLLGVWMIFRRIEAPKRVLIGGARG"
     gene            complement(1280..2353)
                     /locus_tag="VNW28_04010"
     CDS             complement(1280..2353)
                     /locus_tag="VNW28_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HXA09120.1"
                     /translation="MIPHDKLFTPGPVEVSGKTLAAFSRPMIGHRGDDFKTLYRAVHP
                     RLQELFGTKQPVFLSTSSAWGVMEAAIRNLVRQRVLCCMCGAFSDKWLEVARRCGKEA
                     EPLQVEWGQAIDHKAVDAQLATGKFDTLTLIHNETSTGVMNPLPEISCTLAKYPEVAL
                     IVDCVSSFSAVPIPMDAYQIDALLTGSQKALALPPGFALFSISEKGFARAAQAPDRGY
                     YFDFLEFKKQQDGGMTPTTPSIGHIYALQSKLDDIFAEGLTNRYERHARTNALVHEWV
                     RARGFEFFAAEGHRALTLTCVKNNRGIDVAKLTRDLREKHHLVIDGGYGKLKGHTFRL
                     SNMGDETEETVAHLLRCLDDCLG"
BASE COUNT          441 a          752 c          752 g          548 t
ORIGIN      
        1 cgccccggag aaatccgggg aacagaattc ggcttatcgc catcgaaatg aagggcgcct
       61 tcggaaacga gggcgccctt ccactttttt tcgcctgact ttaaccgcag aggacgcaga
      121 gagcgcagag ggaagacgaa atacttgccg gaactctgcg tcctctgcgg ttaatcatcc
      181 ggcattgctt tacggaatcg catcgcaacg cgagtgtcgc gccaactcat gccagcgacc
      241 gtccacgctt ctcgagattg gcgcattccg gccgcctggc tcacgctctg cgtcgtctgg
      301 agttcgactt ggctggtgat caagatcggg ctacgcgatc tgccgccggt ttcatttgtc
      361 gcctggcgat tcgtcgtcgc ggtgctcgtg ttgctcgcca tctcgatcgg ccgcacgcac
      421 ctcttgccgc gacgcggctc ggattatctc gtgctcggac tcaccggtgt gctgatgttc
      481 tcggtcaact acgcgcttct cttctgggca gaactgcatg tctcgtccgg attggcggcc
      541 gtcatccagg cgtcgatccc gatcttcgga atgatctttg cccattggct gttaccggag
      601 gaaccgctgc gttggcaacg tctggccggc gccctggtcg ccatcttcgg agtcgggcta
      661 atctgcgcgc gcctcctgag tttcaatggc tggctggctt ttctcggcgg gctcgggatc
      721 accatcgggg cggcgagcgc cgccttttcc aacgtcctcc tgaaggccag aaaattgcag
      781 ctcgcgccct cgatgctggc ggcctggcag atgatttttg ggatcgtacc gctcttactc
      841 attggtctga ggttggatgg caatccactg ctcttccatt ggactggccg cgccatcttc
      901 tgcttactct atctcgcggt cgttggctcc tcctttacgt tcctgctgct ctactggctc
      961 atgccgcgga tgtcagtaac caatctgcag accatctcgc tcattacgcc gccgggcgcg
     1021 attgcgctgg gctgggcgtt aggcgacgaa cggctttccg cctggtcgct cctcggcgcg
     1081 gggttcgttc tgctcggcgt ctggatgatc tttcgccgaa tcgaggcgcc gaagcgcgtt
     1141 ttgatcggcg gagcgcgggg gtgaagttga ggaacgccat ggagagacgg gcgctgtccc
     1201 gtccgtgttt cagaatctcg gccgacacgg cggtcgtccc tccagttgct cgccgcgatc
     1261 agtctttggc ctaacgattt tatcccagac aatcgtcgag acaacgcagg agatgcgcga
     1321 ccgtctcctc ggtttcatcg cccatgttgg agagacgaaa ggtgtggccc ttgagtttgc
     1381 catatccgcc atcgatcacg agatggtgct tctcgcgcag gtcgcgggtt agtttggcga
     1441 cgtcgattcc gcgattgttt ttcacgcaag tgagggtgag tgcgcggtgg ccctcggccg
     1501 caaaaaactc gaagcccctc gcgcgcaccc attcgtggac gagcgcgttc gtgcgcgcgt
     1561 ggcgctcgta gcgattcgtt aggccttcgg cgaagatatc gtcgagtttc gactgcaacg
     1621 cgtagatgtg gccgatgctc ggcgtggtcg gcgtcatccc cccatcctgc tgttttttga
     1681 attcgagaaa atcgaagtaa tagccgcgat cgggtgcctg cgcggcgcgc gcgaacccct
     1741 tttcggaaat gctgaagagc gcaaatcccg gcggaagcgc gagtgctttt tgcgagccgg
     1801 tcaggagcgc atcgatttgg taagcgtcca tcgggatcgg cacagccgag aaggacgaaa
     1861 cgcaatccac gatcagcgcg acctccggat atttcgccag cgtgcaggaa atctccggca
     1921 aaggattcat caccccggtg gaggtttcgt tgtggatgag agtaagggtg tcgaacttgc
     1981 cggtcgcgag ttgggcatcg accgccttgt gatcgatcgc ctgcccccac tcgacctgca
     2041 gcggctccgc ttccttgcca cagcgccgcg cgacctccag ccacttgtcg gaaaacgcac
     2101 cgcacatgca gcagaggacg cgttgcctga ccagattgcg aatcgccgct tccatcacgc
     2161 cccaggccga ggaagtggaa agaaaaaccg gctgtttggt tccgaatagt tcctggaggc
     2221 gcggatgaac ggcgcggtaa agcgttttga aatcgtcgcc gcgatgtccg atcatcggcc
     2281 gggaaaaagc cgcgagcgtc ttccccgaga cctcgaccgg accgggcgtg aaaagcttgt
     2341 cgtgtggaat cacggcagaa acgtccaacg tccaacgtcc aacgtccaac attcaacggg
     2401 gccgcgccgg gaaggggctt gcgcggaaaa ccttcgcgtg gcatagacac aactcccgat
     2461 tttccatggc cactgtatcg caaatgaccg ccg
//
LOCUS       DATMNS010000112        35420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5167859, whole genome shotgun sequence.
ACCESSION   DATMNS010000112 DATMNS010000000
VERSION     DATMNS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 35420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35420
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5167859"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..964
                     /locus_tag="VNW28_04015"
     CDS             425..964
                     /locus_tag="VNW28_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09121.1"
                     /translation="MPSDFRPLPHEHWQAGDALHRPPRIRTFGFEHECFSATQVSEQY
                     FEDTVSRILFAFGVDGDRTVEEQHAAHEFGRGLRAQAIWIEQSQFSRELFYSRAGLAK
                     QDHGCRSTEKLSEVHAVMGEGLAQPGPVVPRTRAVVFSQRAIAAKMTTATGSATAQNT
                     IAPPDKTISVKIDFIFFLG"
     gene            complement(1610..2431)
                     /locus_tag="VNW28_04020"
     CDS             complement(1610..2431)
                     /locus_tag="VNW28_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HXA09122.1"
                     /translation="MRRLVRWNLSSRARQLSGGDAILVSIPKSGRTWVRTFVCAYFCE
                     KIGQPFTLEPEQYEGVPHIIYTHDLFEQRTKADRWDRLRGKYLIPQRERNRLPIILLA
                     RDPRDAFVSLYIQLTRRTKETPNELKEKSATELLRDAAFGIQSMVDIMNSWLAEWSGR
                     ANFLLLRYETLRQAPVAGFREVLQILGECRVEEKALAHALEFSAFGHMQKLEGAGAFD
                     SKILRAGDAGDPESFKVRRGKIGGFQDYLSPNDQAYAAAALRRLDSRFGYDANSP"
     gene            complement(2428..3222)
                     /locus_tag="VNW28_04025"
     CDS             complement(2428..3222)
                     /locus_tag="VNW28_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09123.1"
                     /translation="MTELKDFQGSVALVVSSCDAFFDAWRPFGAFWQKFWPDCPLEAF
                     LLTNELHIRSSRIRSLAVGGDRGWSSNLLAALERIAHPYVLYLQEDYFLTAPVDGVQL
                     ARDFSEVIESGAESLCFRARSEPDPGFRALNDRFGIVPLDSDGRTRCQVTLWKKSALQ
                     SILRRGETAWNFEARGSARTQEMQILSYRSRENTPLPYLMSAISRGLWMPEAIALCRA
                     HDVQIDPFFRPIYSPGRWQQRLRRTLGRARLRGALRAQSNRVITLP"
     gene            3416..3772
                     /gene="ssrA"
                     /locus_tag="VNW28_04030"
     tmRNA           3416..3772
                     /gene="ssrA"
                     /locus_tag="VNW28_04030"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            3894..4631
                     /locus_tag="VNW28_04035"
     CDS             3894..4631
                     /locus_tag="VNW28_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXA09124.1"
                     /translation="MGGAFMTTHWSMVTDFTSQQASPDRAQAALTRLCQDYWPPLYRF
                     VRLRGYSRDDAQDLTQGFFAYLCEKQGHTAPDRGKGRFRTFLLVLLKRYLGATHLHHC
                     RQKRGGGKFHLFLDAEVMALHEIDDSALVTAPPLDEEQLFERNWAAALVARAMSALSE
                     EYSSGPKARLLSELRPFLTGGIGLPTHEEAAARLGVPLETLRSHLFRLRARYRALLRA
                     EVSRTVPRPEDVEGELRYLCRVLIAGA"
     gene            4719..7553
                     /locus_tag="VNW28_04040"
     CDS             4719..7553
                     /locus_tag="VNW28_04040"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXA09125.1"
                     /translation="MVGDSVCLHCLLRGGIAADDGETAVSPADFGRRFEHYEILARAD
                     GSPWELGRGAMGVTYKAIDVNLRVPVALKVINGRFSGQPGARQRFLREAQSAAQLWHP
                     NVASVFHFGTVNLLPEPNGTTNAAGENIEVGDCFYAMEFIEGETLEKRLRRSGPLTPL
                     LALELAVQVTRALAAAERRGLVHRDLKPSNIMLQAEEGGALGNDRRRASAEAWVKVID
                     FGLAKAATEADEPPASAHFLGTPQFASPEQIGGDEVDGRSDIYSLGSTLWYSLTGKVP
                     FPNRSASELHRGAASPPPPVAQLVERNIPPSLIALLESMLANNPEDRPASAVALGEAL
                     QNCLENLAGVDSPAGASLRHGGRKRILAGALGLAAILAGLAVYFFWFAPAGNDKSIAV
                     LPFENLSNDPANAFFAEGIQDDLLSRLVKIRDLRVISRNSAARYPANARRDLRDIGRT
                     LGVRHVLEGSLRRTGDHVFLHVALIDTHDGHELWGERYARSLADAINLQGELASSIAD
                     ALDATLSVRERMDLQSRPTRNADAYVLYFRGRKLESGPAGAIANFEAAQALYSQAIAL
                     DPGFALAHARLASALSMLYRNRGPSEDLKRRAHAEARAALHLQPDLGEAHLANALCAY
                     RIERDYDRALPELMIARRLMPNDTEPESFVAFIHRRQGRWREARAGLERICALDPRNM
                     SYEEELYATCCLTRDWAGTAVHAARAEALAPTLPSLKIERAMVELWKHGNLAPVQDAL
                     KNIAAASDPEGNVAWALWDGAMLSRDFGAAQTAIDGFPFETLPSVFGAPLPKSYLEGC
                     IQLAQGNSSRAEELFEDARPSMEAEVVAHPDDGLRHARLGLLYAYMGRKAEARREGER
                     AVQLQPPARDAYDGTERVCNLALIYARVGDADQAITMIESLLRKPGAIAFYEASMSLS
                     ELRLRWQWDPLRADPRFQKILENPEPPTVY"
     gene            complement(7802..9652)
                     /gene="ccsA"
                     /locus_tag="VNW28_04045"
     CDS             complement(7802..9652)
                     /gene="ccsA"
                     /locus_tag="VNW28_04045"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXA09126.1"
                     /translation="MKTARSYVIWLIVAGALAYVAAPLFSHDTPGGFEMQKFGRLPVL
                     LNGRIKPLDTVARNSLLIIHGQQTLGTEHDTLTATDWLAELLMKSDSADPRKIFVIRN
                     PETLAALGWTIEAGKYFSFRDLFTHLQEVEQQAALAEKVDVQLRSPFQRDIIKLYERL
                     TLYHQLENSLEVAGTVDFKSQVDDLLKNIRPSPIPMNSGISSEALQSIGFLAEAGYFF
                     PIPPVPPNDDPLRWRKMGESILQFITAGNMHTAVSAYATMASALAANDPATFNRALDD
                     YAGWLQQHFPARVWKAKVESVFNQLQPFYSAMVIYVLIFILAAASWLVWPETLGRYAF
                     ALLVVTFVVHSGGLITRMYLEGRPPVTNLYSSAVFIGWGAVLLGIFLERFFRNGIGSA
                     TAAMIGFITLLIAHHLSMDGDTMEMMRAVLDTNGWLATHVVCVTLGYASTFLAGFLAL
                     TYIVRGAFTPSLDRDTASSLARMVYGIVCFATLFSFVGTILGGIWADQSWGRFWGWDP
                     KENGALLIVLWNAIILHARWGGLVRQRGLMVMAVFGNVVTSWSWFGVNMLGIGLHSYG
                     FMDSAFPWLIGFGASQVVFMMIGVLPPNVWRSQLETAPKPSGAGMVDVPA"
     gene            complement(9649..10815)
                     /locus_tag="VNW28_04050"
     CDS             complement(9649..10815)
                     /locus_tag="VNW28_04050"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HXA09127.1"
                     /translation="MSTATSGFQNSPFRVLASLRLTVVCLGLGMLLVFLGTLAQVHLG
                     IHAVQARYFQSLLIFWSPPGASWTIPILPGGYLLGTVLLLNLLAAHATRFQFSRKKIG
                     IILLHFGVILLLIGQLLTGLFARETQMRIDEGQTLGYSEAPREVELAVIDVSDPKFDQ
                     VVAIPERVLARGGTIQNPTLPFTLNIRQFLANSHLVMRSQEPQAPPSMATTGLGTNIV
                     VAEVPRTVKDDERDLSAAFVEVNGVQGSLGTWLVSNAIPDPQSFTVNGRTYELVMRQR
                     RFYKQFALTLLHFAHDRYAGTDIPKNFSSRVRLVDFERGENREVLISMNDPLRYRGFT
                     FYQAGFDNNDRTTILQVVRNPAMLLPYVACGLVALGLVTQFSMHLFGFVKKRSR"
     gene            complement(10940..12067)
                     /locus_tag="VNW28_04055"
     CDS             complement(10940..12067)
                     /locus_tag="VNW28_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09128.1"
                     /translation="MKPLFPLPCLFVRLPLVAAALLVGAGCGNSDDSKITVYRIPKET
                     QPAAIPPPAAMNNAAPADVHWTAPSGWEEQPASGFRKGSFLVRGADGKTADVSVVSFP
                     EAAGGLLANVNRWRDQLKLAPISNEAEAGSPMSVGGHDMFFVDLVSAQPVAPDGSKAR
                     ILGGIFPANGETWFFKMMGPDQLVESQRAAFQQFLQSVHASEGASAPMPMTANTGGRN
                     TNAPTPPLIAAAQSVPLQYAIPPGWQEKPLSPMRLASFKATSPNGKEIDVSVVSLPGV
                     AGGDLANVNRWRSQVKLGPIDEDALAKSAEHVTANGHDFLTVDLVSDAPIDAQQGKQR
                     ILAAILDENERAWFIKMTGEDAAVTAQKSAFTDFLRGLKIP"
     gene            12209..12667
                     /locus_tag="VNW28_04060"
     CDS             12209..12667
                     /locus_tag="VNW28_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09129.1"
                     /translation="MRSFSRIPSLAAFLGRLALIASLGLGACQQQKVVLTPGENDLVM
                     LNGCVVSACNYLAAVETKHSLEPNFWAKILLVRYKDHPAGHAYCVWETDGTIYGYDRN
                     AGSFPIPVYTREPKLIATVLADGLSKVLKEPLTVAQAEFVEPHKADLYKF"
     gene            13096..13383
                     /locus_tag="VNW28_04065"
     CDS             13096..13383
                     /locus_tag="VNW28_04065"
                     /inference="COORDINATES: protein motif:HMM:NF015378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HXA09130.1"
                     /translation="MKPISLSGLSLLLTLSLLSASPSDRKSGARITKNEAEHIALQHH
                     PGSRVTAAMLETVQGKLVWSIEIMQSQSKLVTAVAVDAMSGQIAAPKKDGH"
     gene            complement(13400..13993)
                     /gene="msrA"
                     /locus_tag="VNW28_04070"
     CDS             complement(13400..13993)
                     /gene="msrA"
                     /locus_tag="VNW28_04070"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXA09131.1"
                     /translation="MFKSLSFTLLAITLCGTALTARAETKTAIFAGGCFWCMQEPFDQ
                     VKGVTKTVVGYTGGSKEDANYTAVSAHRTKHREAIEVTYDPAQISYDRLLDVFWHHIN
                     PTQADGQFHDIGLSYQTAIYFANDDEKKLAEASKEKLAKSGKFDQPIATEVLPAMPFY
                     PAEDYHQKYYLKNPAAYEAYHVGSGRVSYLEKIWGKP"
     gene            13992..14675
                     /gene="trmB"
                     /locus_tag="VNW28_04075"
     CDS             13992..14675
                     /gene="trmB"
                     /locus_tag="VNW28_04075"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HXA09132.1"
                     /translation="MVPVTRDPADQLFHRQLPPTDPMTDANSVGVSPASRFELIPANY
                     FAPLDLAQVFPRVAPLEIDLGCGDGAFLVAMAERFPERNFLGVERMPGRVRSACGRAA
                     RQGLSNVRVLRVESFYAVEYLFPHGSAAVAHLLFPDPWPKKRHQRRRIVTREFVAAVH
                     RLLAPGGQFRVATDQADYFDSIRASLASAAFAEETPDEVDAFPLTTFEIHFRAQGAPI
                     HRLLLRKAA"
     gene            complement(14645..16939)
                     /locus_tag="VNW28_04080"
     CDS             complement(14645..16939)
                     /locus_tag="VNW28_04080"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HXA09133.1"
                     /translation="MNLQDSPRQPLVGVALAAVGGILLAEFFPVPIALVCLCSGAVAV
                     VSLFRPAGWLSHLLVAAAFCALHLIQQTDAPGKDLAARLGGRASSVTVTGTVASEPKA
                     SPNDFTTFLLHLDSIDLRGQLEPSRATVRVRWKGNPGLGDELEVNGSVETIPPARNPG
                     EFDLRGYLARRDVFQSVFARYPEDGTIIRTGGGRFVPRLAARARAWMRTTLNRGLEDS
                     PDVTALINGMALGLRHETPNDIEEPFQQTGTLHLFAVAGLHVGIVAQLLWILAALVRL
                     PRAAAAALIIPCLFFYSAVTGFHVSSVRAATMAAVLLGGIFFGRPVLALNSLAGAALL
                     ILAVDSNQLFTSGFQLSFAVVGAILILQDWIFRGLLWPSESDPFLPRSLVSGRRRVFE
                     HAYRWIAGGVSVSAAAWIGSLLLIIWYFYLVTPISLLANLTVVPVAFCILATGLLSLA
                     TATFSTTLSLVFNHANWSLAKIILAMVQAFAHLPTGHTYVEWPHWPTGARTEITVLDA
                     GTGAAVHLRAPGADWLFDAGSARDYERFLRDYLHSHGIDRLDGLLLSHGDSSHIGGAR
                     AVLDEFRPRRVLDNGARDRSTVHQAFLARLAKLATPREIAARDSVFALSHRVTARILY
                     PVTGFKAKAADDQALVVQLLIDQKFRVLLVSDSGWKTEDALLIRSSDGIRSDILIKGQ
                     NHSGGSGSKEFLDAVQPQLIVATSRDFPTRERIPDTWARTVRERGITLFRQDETGAVK
                     LEFFRDHWQASGFMDHAAFRSKSR"
     gene            17063..18019
                     /locus_tag="VNW28_04085"
     CDS             17063..18019
                     /locus_tag="VNW28_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXA09134.1"
                     /translation="MPTPFSRKKRTNVIRAGAQNGAAKQSATRVSLKPGLDWHKRNFL
                     TEVLIPSLFTRRSGDRTLPLFPKVRPGEICITWIGHASFLIQLEGMNVLIDPNWSMWL
                     KVIKRLKRPGMTIDNLPLIDTVLVTHAHFDHLDRRTLRRVAADQPIVVPTGVGNLVHD
                     LGFNIVHELDEWQQVQLGPLKVSLTPCHHWGARMLHDSHRGFGGFVIEANGRSIFHCG
                     DSAYFSGFTEIGKRFPIEIALLPIGAYDAPSKREVHMNPEEAVRAFLELGGQKLVPMH
                     YGTFRLGFEPLDEPPERLLSSARAHGVEDKVLVMIEGRPVVL"
     gene            18037..20076
                     /locus_tag="VNW28_04090"
     CDS             18037..20076
                     /locus_tag="VNW28_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09135.1"
                     /translation="MNEKTPRRADLFGAAAAGALVLAFCGLLLWHNPLLFWNDDFEIS
                     ILPVFADVARSWSLGQVPLLSPYSWVCGNLAGEFQYGIFSLFVNAAVVLIWKFPLAFA
                     QQAAALSLAHLFVLAAGAFLLGRGRNLSPPLAAMVALVAALNGWIICWGASDWFGALA
                     AFAWLPWAWWGMERALDESRTKFRFLWPAPFVYLLITGGFPYTILMLGLLALWLTAKS
                     LGEARPLSSLGPLALGLALGGGFSAPAWLALFDYVHGSARAAQENPGVHWQWLVPPAA
                     LPGLIWPPWTVLWTDFWTRPVPHAAIELACGTAPPLVLGAALVFLRGRFVRRMKWELG
                     LLVLVLLIAMLPSANVFRWSFRWLPFFHLVLGLLAAEALAQFRLATAWRWLFVLVVFG
                     SLLATYLVLPTHPAVPRYHFASRLTSPQPLDPARLYLSVYPPPETTYRVENHPSPVGQ
                     VTRPGSTSMWAGLHFVNGYSPICGAGVGRAFAFSTHGEIDPGMADYLLGDEAGRDGLL
                     ALLGVDGIVVAHEIAAAPQPATEWTLAREEAEGCIYQRVGGPLPRVRSVASLPSAPNE
                     RLARATIGLRADTRQEIVAEVEVPAGAAPALLVFSRPYFDGYRATVNGKKIAIGSYRG
                     LIPVIKLAPGTQGRLVVIYRPWWLLAGGTIAAASLLVMLSALALAAFFGAVRPAQ"
     gene            complement(20070..21248)
                     /locus_tag="VNW28_04095"
     CDS             complement(20070..21248)
                     /locus_tag="VNW28_04095"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF004859.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite reductase subunit alpha"
                     /protein_id="HXA09136.1"
                     /translation="MPETANPSVSPNDQPAASETYSRKNPFPGRLLVNRRLNSPDSEK
                     DIRHFEISFGDSGLTYEVGDSMAVYPTNDPGLVAEILEALAATGEEKIAGNRGAPTTL
                     REALLRDHGITQPTPKFLKAIAQRASAAPLLNELLEPKRKQDLDTYLWGMEVIDFLVE
                     HPSVKFEPQEFVALLPKLQPRLYSIASSLKVFPEATHFIVDVVTYTSHGRLRKGVCST
                     FLAERCADSPAPIVPAVSKFRLPEDRDAPLIMVGPGTGVAPFRAFLQERQAIGARGRT
                     WLFFGAQHEKSDYYYQEDFERFIADGFLTRIDTAFSRDQAHKIYVQDRMLEAAEELWK
                     WLDEGACFYVCGDAKRMAKDVDAALRQIVQRQGGKSVEAASEYVEKLKNDKRYKRDVY
                     "
     gene            21367..22128
                     /locus_tag="VNW28_04100"
     CDS             21367..22128
                     /locus_tag="VNW28_04100"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HXA09137.1"
                     /translation="MPRSLRRRKSDYLLIDISNSFTKLAFASWKKLGGKERIATDRLS
                     VAALGNLIRQRAVEQIVVSSVVPGKNALIRRAAGRIPVTFVSAKCELGVGVDYPAPES
                     IGADRLANAAAVAALFGSPAVVVDFGTAVTFDVVSADRSYIGGVIAPGLEAMTTYLYK
                     RTALLPKLVLAEPRRAVGRSTKAAMMSGAIFGYRGLVKEILVKIGTEAFGHRKAQVVA
                     TGGYAQLIAAQLPEIDAVRPHLTLEGLRIIGNLNR"
     gene            22145..23092
                     /locus_tag="VNW28_04105"
     CDS             22145..23092
                     /locus_tag="VNW28_04105"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXA09138.1"
                     /translation="MNKTAIGIDFGGTTVKSGVVREGKLLARLPPLQTHDHTGHDSLL
                     AAIRAAIEELRRQHPDVAAVGAGLPGLVDSINGRVWQLSNVPGWEDVALTALLEEWTG
                     LPAAIDNDANAMAYAEWLFGAGREGVNVVCVTLGTGVGGGLILDRKLYRGSRLGAGEI
                     GQMTIDPKGVPGQYGNFGALEKYVGNAQIAERAQTLYREAGLTKTIEEYSPLALEKAA
                     RAGDPVAVKVWEEIGFHLGITLCDIVWLLNPDRIVIGGGVGRAGEYVFGPIRRVIEER
                     TMKIFHEGLTIVPAALGNDAGMIGSAALALAKATAPNEV"
     gene            complement(23079..23558)
                     /locus_tag="VNW28_04110"
     CDS             complement(23079..23558)
                     /locus_tag="VNW28_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HXA09139.1"
                     /translation="MIDWATWQPRQRASLCFIVREEMILLIRKKRGFGAGKINGPGGK
                     VDPGETPLASALRETYEEVGIKPLGAEQRGELHFQFRDGYSLHCTVFLAHDFTGKPRE
                     TDEALPLWTPLDAIPYDEMWADDRHWLPLLLRGAHLRAWFEFDGERLLRRKIVVRPR"
     gene            23619..25460
                     /locus_tag="VNW28_04115"
     CDS             23619..25460
                     /locus_tag="VNW28_04115"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA09140.1"
                     /translation="MKISESGLIFLLVLSSFAANVVAPTKSELEAMYAAAARELNAGH
                     YSEALKELDAIDARQPDMAAAQNLRGIALMRQAEYRKAETALHRALEIDPEFWEASFN
                     LAEVSFLMQNWTEARRRFQALLAEGTEQVQGATRDLIQFKILLTFLLEGKEKMGAATL
                     EKLQASHDSPVDYYVKAALALRQKDQQNAKISMAAAEKEFSPQLNKLFAESFYEVGWL
                     QKPDGATPVALEIGSPAERVAQVQENFGKAERAYRQRDFASALRLLDQIDATAPNQAV
                     AYNLRGEILLEEGKADAAEAALRIALAADPQLQEARFNLARVPFKKGDYKTARQELEA
                     LLGATSGGKQQQQMEQLVRYQIFLTLLLEGRESAAQKAMEEFKMMDDTPALYYAQAAW
                     AFQHGNAMQGNNWVANAKNLYSSELNRSFAASFADLGWLKNSEAQPAQTQSSLAEAEV
                     SPRAEPPPTRPRNENNPMPVPVAAESPTDQPIATTESTATPTPPVALAGIVAASPTLT
                     LTATPNNVASVEAKQPREEQASSAGTKRRRSAGAKSKRHASVAAPSPTPMAQPTRQNF
                     GDKVARLFLYPFRHRSEQGSTPTPAGSDEAAAASANSNPPARSQPKN"
     gene            25503..29006
                     /locus_tag="VNW28_04120"
     CDS             25503..29006
                     /locus_tag="VNW28_04120"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA09141.1"
                     /translation="MKIRRLAAISLLVLGSFASTGLAAEKSSLEAMYDRAFTAFDAAR
                     YDEALKALDAIDARQPDLAESFNLRGVVLMRQGKYDKAEVALRKALSIEPKFWNASFN
                     LAEIPFLKKDWAAARTRFEGLMSGENSGMQPETSQLIQYKILLTFVLEGKENTVDWIL
                     NKFELSKDSPALYYSNAAIALEHKNQAEAKEWMDAAEKKYPGPLNKLYAESFYEIGWL
                     QKPSGESRPALEITSTADRVARMQADAKANFEKAERAFQQRDFDGALKLLDLATAGAP
                     NDAASDNLRGEILMEQKKFDEAEVVLRRAFATDPKFREAQYNLAQIPFKKGEYAKARD
                     RLEALFGETPGDEKNQAAQLIKYKIFLTLLLEGKESEAQQLMEQFKFTGDTPALYYAQ
                     AAWEYKHGHEDQGSDWVTSARKIYSPALNIVFADTFYDLGWLKNPTVATAPPPTSALA
                     QADASPLVEPTPAMRLGQMESRPVPDLTGDGMAKSTDVEAKEPAAPTPAATGLETTAP
                     IATGPVATAAQTTAPTVTAAQTTAPIATAPTATGPETTPGAMAAAVAAATPLATAVPA
                     VSPASKPATSPIAVASSAVNVAAPSLAATQTGASSRSTFGEMIDRVSSPLSPLVGVLL
                     LAGIVLLLWLVMQQIRRYLANNSLFKSSTPLTEPPFAGDEPMVSDEHRISNDILSSGP
                     PKVSLQLKASEPSVRAAVLPSGAITARGAVPGVPEPFNLQPNELEGSIAEVMPAPVSA
                     RPPVEPGPTIEEVLPAAEAKIPAEAEAVLRDQEEVSLPAALEVPAASLASQAAEPAAP
                     DEPVAPVEPVPAQPVAEKSAAEEAAMIAPEIILEPAISEFAGHAWEKIAPELEPVAST
                     PIEEPALPHEEPIGQGQPIPQLTTALAAEAVTAEVTPPTPETPLVLVEPEAAVPPPIA
                     EWQAPQSSIETPSFASKIITTEPIRLQPTTPVIMPETTITPAPAFRAATPTMSVQQPL
                     GGMHTAVQLTFSLEIASMQLTPTFKMSGLQLKPISKVVSMRLAPSQDPQPPMNLQVTF
                     EVAKIELSGGSIGTVRLAPSLQEKPAALSSPSFAISGLELVAGAGTAPVQLTPSHQEQ
                     ASVLLTAHFQIAAIEFSPLFEIAAIVLTSTSKHVSMQLPGSGPSSIDNAPVFQIENVQ
                     LGAGADLNTIQVTPIGTGRLT"
     gene            complement(29065..30174)
                     /locus_tag="VNW28_04125"
     CDS             complement(29065..30174)
                     /locus_tag="VNW28_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXA09142.1"
                     /translation="MQDARFDKLASLLVNYSTALKSHEKVLIESFDAPDEMTIALIRA
                     ARKAGAIPFVQLQRARISRELALGIDARQLDLTAAHELARMKKMDAYIAVRGSNNISE
                     LADVPIDRMKLVAKKLRPLQDQRVQKTKWVVLRWPTPAMAQLAGMSTEGFEDFFFKVC
                     TLDYRKLQPGMKALKTLMEKTDRVEIKGPGTDLRFSMKGIPAIICGGDRNIPDGEVFS
                     CPVKDSVQGHVTFNTPSIYQSTGFDNIRLDFRDGKIVEATSNQTKKLNAVLDSDPGAR
                     YIGEFSLGCNPYVLHPMRDILFDEKIAGSFHFTPGQAYQEADNGNRSQVHWDMVSIQR
                     KDYGGGEIYFDGQLVRRDGEFLPKQLRSLNWTSFK"
     gene            complement(30295..31545)
                     /gene="rsmB"
                     /locus_tag="VNW28_04130"
     CDS             complement(30295..31545)
                     /gene="rsmB"
                     /locus_tag="VNW28_04130"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:TIGR00563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HXA09143.1"
                     /translation="MKESGRSLALKALARWRKGAEFADQIVAEIFAQSALGVSDRAFA
                     LELFYGALRNLTLLDFWIAQLRPAPLEADARDILRLGLYQILLIETAAHASVFETVEL
                     ARPRTRPLINAILRRALRERMALKQQAEEQPLQIRFSAPEFLIAKWSRQFGSAATFEL
                     CRWNNRPAPVYARINELKTTAAEFLRRYAGSFLLPGHSNFVGLPGPADALRNGDCYLQ
                     DPSTALACEMLRPAPGESVLDACAAPGGKSAYLAEMMQNEGALVAADRDEVRLERLRN
                     NLVRLEVTNTRTVRCDCSDENSLREAALARQSFDKILVDAPCTNTGVLRRRVDLRWRL
                     RPEDFVRMPGRQGQILRAIAPLLRPGGSLVYSTCSLESEENDEVVAAFLRAQPAFRLT
                     KREESLPFRDLIDGAFAARLEFSP"
     gene            complement(31602..32915)
                     /locus_tag="VNW28_04135"
     CDS             complement(31602..32915)
                     /locus_tag="VNW28_04135"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXA09144.1"
                     /translation="MNLSIIGSGYVGLVTGACFADVGHNVICVDNDESKVESLQHGKV
                     PIYEPGLEEIVHRNVTAQRLRFTSSIKEGVDNAQVVFIAVPTPQDADGSVDLSFIEKV
                     AREIAGVLTGYRVIVDKSTVPVKTGEKVAESIKRYNKHGAEFDVVSNPEFLREGCAVA
                     DLMKPDRIVIGAQSERAIDLMKKVYEPFMAPMLVTDINSAELIKHAANSFLALKISYI
                     NAVSAICEASGADVEKVADGIGMDRRIGRNFLNAGIGYGGSCFPKDIAAFIMISEQLG
                     VPFNLLKEVQRINAMQKERFLKVVRDALWVLRDKKIAVWGLTFKPDTDDIRSSVAIEL
                     VAEMLREGAQVTAYDPKGMEKAHAVKSIAGARLTNTAMEAIEDAEALIIATEWAEFAN
                     VDLATVREKMHTPMVFDGRNLYDPETMRKLGFHYTSIGRPQARPA"
     gene            complement(33008..35254)
                     /gene="lptD"
                     /locus_tag="VNW28_04140"
     CDS             complement(33008..35254)
                     /gene="lptD"
                     /locus_tag="VNW28_04140"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HXA09145.1"
                     /translation="MRKPVFLLLFVLAVSRAAAEDLEGPGTQPVEINATGRMIYENGI
                     ATAHDNVSIRFGDTDIYSDYAQYDKAKRTVFLKGHVRIYRGVSLYLAEQATYNLDTKA
                     INADSMRSGTFPYLISGKTVNSDSDQEYLVKNGTFTTHDAAQPDFHMSAQSVRIYEND
                     RVIFKSVTFYVGQIPVFWWPYLYQSLDRSFSYSIAPAYLSSWGFALLGQVTFPIGDHI
                     TSTVKLDYRTRRGPAIGFDSEIRYGKGDSSVAKIISYYLQDQNPNLNRTALPREGTPT
                     GRYRLSIQDRTHITDDIEATVDITKLSDAFLLQDFFPAEFRINPQPDNVVAVTKTSPW
                     YTLTVDARFQLNTFFDTTERLPEIALDITRHPLFNGPIFYDGATSVGELNRNFAAGSL
                     FQDYSALRIDSFHQLTYPNTYFGWLAIVPRVGFRETYYSETRNLSLSPLPINPDPNAP
                     EFPLADPTTEPPLHTAGGVFRSVFNAGIEGSFKISQAWDGVQTRALGLDGLRHIIQPF
                     VDYSYVSSPNVDPAEILQFDRVQPSTKLNPIDFPQYTSIDALDNWSIARVGVRNRLQT
                     RRDDLTVSWLELETFVDVNFHDPFEKGDYSNVFNKLTFTPVPWATLVIDSQLPLLANG
                     FTEVDTNINFKPTANTVITLGHRYLNNNPFFLNSSLFTFGGYYRIDDNWGFGILEQYE
                     GATNIFEQQRFSLYRDLTSWVASLGAIIQNNGGVKEYGVLLTFTLKAFPKLGLDLNFD
                     PAGTNQTQ"
BASE COUNT         6890 a        10652 c        10970 g         6908 t
ORIGIN      
        1 aaagatttgc accccccttc gtcacaagcg tcttcggccg acggattccg gcgcctgctc
       61 gtttccgatt ttcttcagag gcgccaaagg cctcacacgc cgcccgagaa gctttcgaaa
      121 tcggcagcca gcgttcgctg cttgcctggc atattattga atcgccggga gtcccgcgcg
      181 gactacaatg cccgcacatt tttgatcgaa gacgtccaac tcggattctt cgatcatttt
      241 tggaggacgg gcgacaaatc gagaaaagtt gcgtgcttgg agggattcga actcccaacc
      301 ttcagattcg aattcagaag aagaatcgaa ttcgactgga tcactacggc actacatcaa
      361 ccagcctgcc ggccggcgag aatccctgcg ctcagtcgag agcttcccag cggtggcttc
      421 gggaatgcct tcggatttcc ggccgctccc gcatgaacat tggcaggcgg gcgatgcgct
      481 ccaccgcccg cctcgcatcc ggaccttcgg cttcgagcac gaatgtttct ccgcaactca
      541 ggtcagcgag caatacttcg aggatacggt cagccggata ctgtttgcct ttggcgtgga
      601 tggtgaccgt actgttgaag agcagcacgc agctcacgaa ttcggtcgcg ggctccgtgc
      661 gcaagccatc tggattgagc agagtcaatt ctctcgtgag ctgttctatt cccgggcggg
      721 gttggcgaaa caggatcacg ggtgtcggtc gactgagaaa ctttcggagg ttcatgcggt
      781 catgggcgaa ggcctcgcgc agcccgggcc ggtggttccc cgaacaaggg cggtggtttt
      841 ttcccaaaga gcgatcgcag cgaagatgac gactgcgact ggaagcgcca ccgcccaaaa
      901 cacgatcgcc ccgccagaca aaacaatttc ggtcaagatt gatttcatat tcttcctcgg
      961 ttgaatgtct actgcttcga tagagattca tactctactt cgcaagtaga gatggcaagc
     1021 actattttcc aaaaattttc aggcgcgaat attgttgggt ggcgggtcgc gattgtcagg
     1081 cggtgctggc ggaaggcccc atcaggcagc ccgggccggc cgcccggaaa agcgcggctc
     1141 cgattttttc gcaaagagcg agcgcaggaa aaacgacgat tgcggccgga agagccaccg
     1201 accaaaacat aatcgtccca gcaagtagaa cagtttccgt caagattgat ttcatatttg
     1261 tttccttcct tttaaatgtc cactaatcca atagagatta atactctact aaatccatag
     1321 agatggcaag caaatatttt cataattttt ttggcggcga aattcccctc ggaaaatgcg
     1381 ccttcttcgg gaggccgctc tgactaaccg cgcggagttg gagcagacgg tcgacgattc
     1441 ccggcttgct ctttcagggg attgcaactt gttctggtcg gcgggaacgg aacagttgcc
     1501 gaataaaccc accgccgcat ccggttcccg agctttcgct gcggctcgac caccgtggcg
     1561 gacacgatgg tgctgagtga gaaggattaa caactcgcgt ccgaacgctt cagggcgagt
     1621 tcgcgtcgta gccaaagcgg gaatcaagcc tgcgcagcgc cgcggcggcg taggcctgat
     1681 cgttaggcga aagataatcc tgaaagccgc cgattttgcc acggcgaacc ttgaatgatt
     1741 ccggatcacc cgcgtctccg gcacgtagga ttttggaatc gaacgcgccg gcgccttcca
     1801 gtttctgcat atggccaaag gcagaaaact cgagcgcgtg ggccaacgct ttttcctcca
     1861 cgcgacattc gcctagaatc tgcaggactt cacgaaaacc ggcaaccggt gcctggcgca
     1921 gggtttcgta gcgcaggaga agaaagttcg cgcgtccgga ccattcggcg agccagctgt
     1981 tcatgatgtc gaccatcgat tgaatcccaa aggctgcatc gcgcagcagt tccgtcgcgg
     2041 atttttcctt caattcgtta ggcgtctctt tcgtgcgccg ggtgagctgg atgtaaagcg
     2101 aaacgaacgc gtcccgcgga tcgcgcgcca gcaggatgat cgggaggcga ttccgctccc
     2161 gttgcgggat cagatacttg ccgcgcagtc tgtcccagcg gtccgctttt gtccgctgct
     2221 cgaaaaggtc gtgcgtatag atgatatgcg ggacgccttc gtattgctcc ggttcgagag
     2281 taaatggctg gccgatcttt tcgcagaagt aggcgcagac gaacgtccgc acccaggtcc
     2341 ggccactctt cggaatcgaa acgagaatcg cgtcgccgcc agaaagctgg cgcgcgcggc
     2401 tgctcagatt ccatctcacg agtcgtctca tggcaaggtg atcaccctgt tgctctgcgc
     2461 gcggagcgcg ccccgaaggc gcgcgcggcc gagggtgcga cgtaagcgtt gctgccagcg
     2521 gccgggggag tagatcgggc ggaaaaaggg atcgatttgc acgtcgtgag cacgacagag
     2581 cgcgattgcc tccggcatcc aaagcccgcg tgagatcgcc gacatcagat acggcagcgg
     2641 tgtgttctcg cggctgcggt aggaaaggat ctgcatctcc tgcgtgcgcg cactgccccg
     2701 cgcttcaaag ttccacgcgg tttcgccgcg acgcaggatc gactgaagcg cgctcttttt
     2761 ccagagcgtg acctggcacc gcgtccgccc atcagaatcg agcggcacga tgccgaagcg
     2821 atcattcagc gctcgaaaac cgggatcggg ctcggagcgc gcgcggaagc aaagggactc
     2881 ggccccagat tcaatcacct ccgaaaaatc gcgcgccagc tgcacgccgt cgaccggggc
     2941 cgtcaagaag tagtcctcct gcaaatagag cacatagggg tgcgcaatcc tctctagtgc
     3001 cgcgagcagg ttgctcgacc acccgcgatc accgccgact gcgagcgagc gaatccgcga
     3061 agagcgaatg tggagttcgt tagtgagaag aaaagcttcc agcgggcaat cgggccagaa
     3121 tttttgccag aaggcgccaa agggtcgcca cgcatcgaaa aacgcatcgc agctggaaac
     3181 aacaagcgcc acggagcctt ggaagtcttt cagctccgtc atggagaatt cgtctcgcac
     3241 agtcgctccg cgagcaagga ctttcacgag cagtgttttc gctggggatt ctgagctttc
     3301 tgctcctgct cgtgatcttc gtcgagtgcg atcgatcacg agcacgagca cgaggcaagg
     3361 cagacgggcg ctattgtaat tccgcctcat tcgggtaaat tgagacctcc aaaaaggggg
     3421 cgtactggtt tcgactgatg gtttgaagcg caggcggcat gccgaggatg gttcgttggc
     3481 ctcgttaaaa tccggaccaa aaaaaataaa tgcagatcac caagatctcg ctctcgcggc
     3541 ttaattgacc gcgatgttcg gagttggacg cctgctacgg cgccgaacat ggacagcagg
     3601 ctagtcggac ggcggagtga ccgcgccgga cggtgaagga tttcgtggtg gctcggtgga
     3661 aggcgtgtgc cggtgcaacg tccgtcctct taaaaataat caccgtctaa gcatgtagaa
     3721 gctcgcggag agagcgatca ggacaggggt tcaactcccc tcgcctccac catagcgaag
     3781 ttcgctcgga aatagaggtt gcgctgctcg cgaaagcgaa gtcttgacta tccgaggccc
     3841 tccccgctac tctcggcgcc gggatggaca attccgttac aaattttccc accatggggg
     3901 gagctttcat gaccacccat tggagcatgg tcacggattt tacctcccag caagccagtc
     3961 ccgaccgcgc gcaggcggcg ctcacgcgac tttgccagga ttactggccg cctctctata
     4021 ggtttgtccg tttgcgcggc tatagcagag acgatgcgca ggacttaacc cagggttttt
     4081 tcgcctacct ttgcgaaaag caggggcaca ccgccccaga tcggggcaag ggcaggtttc
     4141 gcacctttct cctcgtgttg ctgaagagat atctcggcgc gacccacctt caccactgcc
     4201 gacagaaacg cggcggaggc aagtttcatc tgtttctcga tgctgaggtc atggcgttgc
     4261 atgaaatcga cgacagcgct ctcgtgacgg caccgccgct cgatgaggag caactgtttg
     4321 aacgcaactg ggcggccgcg ctggttgctc gtgcgatgag tgccttgagc gaggaatatt
     4381 cgagcggtcc caaggcccgc ctcttgtccg agttgcggcc gttcctgact ggtggaatcg
     4441 ggttgccgac gcacgaggaa gcggcggcgc ggctgggcgt tccgctggag accttgcgca
     4501 gtcacctctt tcgtctgcgg gcgcgctacc gggcgttgct ccgtgcggag gtgtcccgca
     4561 cggtgccgag gcctgaggat gtcgagggag agttgcgcta tctctgccgg gtgttgatcg
     4621 cgggcgccta gttttggctg gccagccgtc cttttgtgaa catttcgtcg gcttctggga
     4681 cgctccgctg cgagcagtgc ggagcaccat tcccgagcat ggtcggggac agcgtttgcc
     4741 tgcactgttt gctccgcggt gggatcgcgg cagacgacgg ggagaccgcc gtttcgcccg
     4801 ctgatttcgg gaggcgattt gagcattacg aaatcctggc ccgcgctgat gggtcgcctt
     4861 gggagctggg tcgcggcgcg atgggcgtca cctacaaggc aattgatgtg aacctccggg
     4921 ttccggtcgc tctcaaggtt atcaatggtc gcttctctgg ccagccgggt gcgcgccagc
     4981 gtttcctgcg tgaggcgcag tcagccgcac agctttggca ccccaatgtg gcgagcgttt
     5041 ttcatttcgg cacagtcaat ctcctgccgg agccgaacgg gaccacgaac gctgccgggg
     5101 agaacattga ggtcggcgat tgtttctacg cgatggaatt cattgaaggg gaaacgctcg
     5161 aaaagcgttt gcgccgcagt ggccctctca caccgctcct cgcgctcgag ctggcagtgc
     5221 aggtgacccg cgctctggcc gccgcagaac ggcgcggatt ggtccatcgc gatctcaagc
     5281 cctccaacat catgctgcag gccgaggaag gaggcgcgct gggcaacgat cgacgccggg
     5341 cttccgctga agcctgggtg aaggtcatcg atttcggcct tgccaaagct gccaccgagg
     5401 cggatgagcc gcccgcgtcg gcccatttcc tgggcacccc gcagtttgcc agcccggaac
     5461 aaataggggg cgacgaggtg gacggccgct ccgatattta ttcgttaggc agcacccttt
     5521 ggtattcgct gacgggcaag gtgccgttcc cgaaccgttc tgcgagcgag ctgcaccgcg
     5581 gggcggcgag cccgccgccg ccggtggcgc aattggtcga gcggaatatt cccccctcat
     5641 tgattgcgtt gctcgagtcg atgctggcaa acaaccccga ggatcgccct gcatcggccg
     5701 tcgcgctggg cgaagcgctg cagaattgcc ttgagaacct cgcaggcgtc gattctcccg
     5761 ctggcgcatc cctccgccac ggcggccgca agcgcatcct ggcgggcgca ctcggtttgg
     5821 ccgcgatctt ggccgggctg gcagtttact ttttttggtt cgcccctgcc ggaaacgaca
     5881 agtcgatcgc cgttctgccg tttgagaact tgagcaacga tccggccaac gcttttttcg
     5941 ccgagggtat tcaggacgat ctgctttctc ggctggtcaa gattcgcgat ttaagggtga
     6001 tcagtcgtaa cagtgctgcg cgttatcccg ccaacgctcg ccgagatctg cgagacattg
     6061 gccggacact gggcgttcgc catgtcctgg aaggcagcct gcgtcgcacg ggcgaccacg
     6121 tgttcctcca tgtcgcgtta atcgacacgc acgacggaca tgagctttgg ggcgagcgtt
     6181 acgcccgatc gctggctgat gcgatcaatc tgcaaggtga gctggccagc tcaatcgcag
     6241 acgccctcga cgccacgctg agcgtgcggg aacgaatgga cttgcagtcg aggcctaccc
     6301 gcaacgcgga cgcctatgtt ctctacttcc ggggacgcaa actggagagc ggtccggccg
     6361 gcgcgatcgc gaacttcgaa gcggcgcaag cgctctacag tcaggcgatt gccctcgatc
     6421 ctggctttgc cctcgcccac gcgcgcctcg cttccgccct gagtatgctc tatcggaatc
     6481 ggggtccgag cgaagactta aagcggcgtg cacacgcgga ggcgcgggcc gcgctccacc
     6541 ttcagcctga cttgggcgag gcgcacctcg cgaatgctct ttgcgcctat cggatcgagc
     6601 gcgattacga tcgggccttg cccgaactca tgatcgcgcg ccggcttatg cctaacgaca
     6661 ccgagcccga aagtttcgtg gctttcatcc accgtcggca gggccgttgg cgtgaggctc
     6721 gggccggact ggagcgaatc tgtgcccttg atccacgcaa catgagctac gaggaagagc
     6781 tttatgccac ctgctgtctg acgcgtgact gggcgggcac agcagttcac gcggctcgcg
     6841 ccgaggctct cgcgcccacg cttccctcgc tcaaaataga gcgagccatg gtcgaactct
     6901 ggaaacacgg gaacctggcg ccagtccagg atgccttgaa gaatatcgct gccgccagcg
     6961 acccagaggg caatgtagcc tgggcgcttt gggatggggc catgctctcg cgtgatttcg
     7021 gggcggctca aacggccatc gacggctttc cgtttgagac tctgccatcg gtttttggcg
     7081 cgcctttgcc caagagttat cttgaaggct gcattcagct ggcccaaggc aacagctccc
     7141 gcgccgagga actctttgaa gacgcgcggc cgtcgatgga ggccgaggtg gttgcgcacc
     7201 cggacgacgg cctgcgtcat gcccgactcg gtctgctcta cgcctacatg gggcgaaaag
     7261 ctgaagctcg ccgggaaggc gaacgagccg tccaattgca gccgcccgct cgcgacgctt
     7321 acgacggcac agaacgggtt tgcaacctgg ccttgattta tgctcgtgtc ggcgacgccg
     7381 atcaggcgat caccatgatt gaatctcttc ttcgcaagcc gggcgcgatc gccttttacg
     7441 aagcgagcat gtcgctctca gaactccgtt tgcgctggca gtgggatccg ttgcgggccg
     7501 atccgcgttt tcagaaaatc ctcgaaaatc cggagccgcc aaccgtctac tagccacgcc
     7561 ccatccgacg ccgtttgggg aaagactccg aaaacgaaag gcgcaagcgc tgttgcgtcc
     7621 aaagatcttt cggatttctg catttattct gcaacgccgc gtgaattttg cgcattggat
     7681 gggtagcgac agtgagttgt cccggtacca tggctagttc ccggatcccc ggatccgacg
     7741 gtgcctgaag gcgcgccccc cgcgccttca ggcgcgatgg gcgactctcc agcagctcga
     7801 ttcaggcggg aacgtcgacc atccctgcgc ccgacggctt gggtgccgtt tcgagctggc
     7861 tccgccagac gttcggcggg agcacaccga tcatcatgaa gacgacctgg ctggcgccga
     7921 agccgatcag ccacgggaag gcgctatcca tgaagccgta ggaatgcagc ccgatgccaa
     7981 gcatgttcac tccgaaccac gaccagctcg tgaccacgtt gccgaagacg gccattacca
     8041 tcaggccgcg ttgcctcacg agcccgcccc aacgggcgtg caggatgatg gcgttccaga
     8101 ggacgatgag gagcgcgccg ttttccttcg ggtcccagcc ccagaagcgg ccccacgatt
     8161 ggtccgccca gatcccgccg aggatggtgc cgacaaaact gaagagcgtc gcgaaacaaa
     8221 cgatcccgta aaccatccgc gcgagggagc tcgcggtgtc gcgatcgagc gagggtgtga
     8281 acgcgccgcg cacgatatag gtgagggcga ggaacccggc aaggaaggtc gaggcgtagc
     8341 cgagcgtcac gcagacgacg tgggtcgcga gccagccgtt ggtgtcgagc accgcgcgca
     8401 tcatttccat cgtgtcgccg tccatcgaga gatgatgcgc gatgaggaga gtgatgaagc
     8461 cgatcatcgc cgcggtcgcg ctcccgatgc cgttgcggaa gaagcgctcg agaaaaatcc
     8521 cgagcaagac cgcgccccag ccgatgaaga cggcggacga ataaagattc gtgaccggcg
     8581 gccggccctc gagatacatc cgcgtgatca gcccgccgga atggacgacg aacgtcacca
     8641 cgagcagtgc aaaggcgtaa cgcccgagcg tctccggcca gacaagccag gaggccgcgg
     8701 cgagaatgaa aatcagcaca taaatcacca tcgcgctgta aaacggctgc aactggttga
     8761 agaccgattc gaccttcgcc ttccagacac gcgccgggaa atgctgctgc agccagcctg
     8821 cgtaatcatc gagcgctcgg ttgaaggtcg ccgggtcgtt ggccgccaaa gcgctcgcca
     8881 tcgtcgcata cgctgatacc gcggtgtgca tgttgccagc ggtgatgaac tgcaaaatgc
     8941 tctcgcccat cttacgccag cgcagcggat cgtcgttagg cgggaccggc ggaatgggga
     9001 aaaaatagcc ggcctccgcg aggaaaccga tgctctgcag cgcttccgac gagatgccgc
     9061 tgttcatcgg gatcggcgat ggccggatat ttttcaggag gtcgtccacc tggctcttga
     9121 aatcgaccgt cccggcaacc tcaaggctgt tctccagctg atgataaagc gtcagccgtt
     9181 cgtaaagctt gatgatgtcg cgttgaaacg gcgaacgcag ttggacgtcc accttctccg
     9241 caagcgccgc ctgttgttcg acctcctgaa ggtgggtaaa gaggtcgcgg aacgaaaaat
     9301 atttgcctgc ctcaatggtc caacccagtg cggcgagcgt ttccggattg cgaatcacaa
     9361 aaatcttgcg cggatcagcg ctgtccgact tcatcagcag ctcggcgagc caatcggtcg
     9421 ccgtcagcgt gtcgtgctcg gtgccgagcg tctgctggcc gtgaataatg aggagcgaat
     9481 tgcgcgcgac cgtgtcgagc ggcttaatcc ggccattgag cagcacgggc agccggccaa
     9541 acttctgcat ctcgaaaccg cctggtgtgt cgtgcgagaa aagcggcgcg gcgacgtagg
     9601 cgagcgcccc ggcgacgatc aaccaaatga cgtaagagcg cgcggttttc atcggcttct
     9661 ctttttcacg aagccaaaga ggtgcatcga aaactgggtt acgagcccga gcgcgaccag
     9721 cccgcacgcg acgtaaggca gaagcatggc cggattcctg accacttgca ggatggtcgt
     9781 ccggtcgttg ttgtcgaagc cggcctggta aaaggtgaag ccgcggtagc gcagcggatc
     9841 gttcattgaa atgagtactt cgcgattttc gcctcgctcg aaatcgacca ggcgcacgcg
     9901 gctggaaaag tttttcggaa tgtccgtccc ggcataacga tcatgcgcga agtgcagcag
     9961 ggtgagggca aactgcttgt agaaacgccg ctgtcgcatc accagttcgt aagtgcggcc
    10021 gttgacggtg aaggattgcg gatcgggaat cgcattcgag acgagccacg tgcctaacga
    10081 accctgcacg ccgttcacct ccacaaaagc cgcgctcaag tcgcgctcgt catccttcac
    10141 cgtgcgcggg acttcggcga ccacgatgtt cgtccccaag cccgtcgtcg ccatcgaagg
    10201 cggcgcctgc ggttcctgcg agcgcatcac gagatgcgag ttggccagga attgccggat
    10261 gttgagcgta aacggcagcg tcggattctg gatcgtgcca ccgcgcgcca gcacccgctc
    10321 gggaatcgcg accacctgat cgaacttcgg atcacttaca tcgatcaccg ccagctcgac
    10381 ctcgcgcggc gcctcggagt aaccaagcgt ctggccctca tcgatccgca tctgcgtctc
    10441 ccgggcgaaa agtccggtca gcagttgccc aatcaaaagc aggatgacgc cgaaatgcag
    10501 gagaatgatg ccaatcttct tccgggaaaa ctgaaagcgg gtcgcgtgcg ccgcgagcaa
    10561 gttcagaagc agcacagtcc cgagcaaata acccccgggc aaaatcggaa tcgtccagct
    10621 cgcgcccggc ggcgaccaga agataagcag gctttgaaag tagcgcgcct gcaccgcatg
    10681 aatcccgagg tgcacctgcg ccagcgtgcc gaggaaaacg agcagcatcc cgagcccgag
    10741 acagaccacc gtcagccgga gcgaagcgag aacgcgaaaa ggcgagtttt gaaaaccgga
    10801 ggtggcagtt gacatttcgt taggcaaatg acggggaggg gaatgacgaa tgacgaatga
    10861 cgaatgacga agcaattcgc gaatgacgaa tggccagcaa tgtctggcag gtaatttggg
    10921 tttcgggctt cgtcattctt cacggaatct tcagaccgcg cagaaaatcg gtgaatgcgc
    10981 ttttctgcgc cgtaaccgcg gcatcttcgc ccgtcatctt gataaaccag gcacgctcgt
    11041 tttcatccag gatcgcggcc aggatgcgct gctttccctg ctgcgcatcg atcggcgcgt
    11101 cgctcaccag atccaccgtg agaaaatcgt gcccgttcgc cgtcacatgt tccgccgact
    11161 tcgcgagcgc gtcctcatcg atcgggccga gcttcacctg gctgcgccag cgattgacat
    11221 tcgcgagatc gccgcccgcc accccaggga gcgagacgac ggagacgtcg atctcctttc
    11281 cgttaggcga ggtcgctttg aagctggcca ggcgcatcgg cgagagcggc ttttcctgcc
    11341 agcccggcgg gatggcgtat tgcaagggca cactctgcgc cgctgcgatc aatggcgggg
    11401 tgggggcgtt cgtgttgcgg ccgcccgtgt tcgccgtcat cggcattggg gcggaggcgc
    11461 cttcggacgc gtgcacactc tgcagaaact gttggaacgc cgcccgctgc gattccacca
    11521 gctgatccgg ccccatcatt ttgaaaaacc acgtctcccc attcgctgga aaaatcccgc
    11581 cgaggatacg cgccttcgat ccgtccggtg cgaccggctg cgcgctgacc aggtccacga
    11641 aaaacatgtc gtgcccgccg accgacattg gcgagcccgc ctccgcctcg ttcgagatcg
    11701 gcgcaagctt caactggtcg cgccagcgat tgacattcgc cagcaacccg ccggccgctt
    11761 ctggaaacga gaccaccgac acatccgccg tcttcccatc cgccccgcgg acgagaaaac
    11821 tgcctttacg aaagccactc gcgggctgtt cttcccagcc cgacggcgct gtccaatgca
    11881 cgtcagcggg cgcggcatta ttcatcgcgg ccggtggcgg tatcgccgct ggctgggtct
    11941 ccttcgggat ccgataaacg gtgattttcg agtcgtcgct gttaccgcat ccggcgccga
    12001 ccaacagcgc cgccgcgacg agcggtagcc tgacgaacag gcatggaaga ggaaagagcg
    12061 gcttcaaggg cagatgcgaa gtacctgccg cgcgccggag atgtcaaggg acgaggccct
    12121 caacccgagg gcgactctcc cggggtatga aagtaatgct tcccaaaaaa gccgcaaagg
    12181 tgctataaca atcataaata tcacactaat gcgctctttt tcccgcattc ccagtctcgc
    12241 ggcttttctt ggccgcctcg ccctcatcgc ttctctcggg ctcggcgctt gccagcagca
    12301 aaaggtcgtt ctcacgcccg gcgagaacga tcttgtgatg ctcaatggct gcgtcgtctc
    12361 tgcctgcaat tatctcgccg cggtcgaaac gaagcacagc ctggaaccga acttctgggc
    12421 gaaaattctc ctcgttcgct acaaagacca cccggccggc cacgcctatt gcgtttggga
    12481 aaccgacggc acaatctacg gctacgatcg caatgccggc tcgtttccga tcccggtcta
    12541 cacccgcgaa ccgaagctta ttgcgaccgt cctggccgac ggtttgtcaa aggtgctcaa
    12601 ggaaccgttg acggtcgcgc aggccgagtt tgtcgagccg cacaaagcgg atctctacaa
    12661 gttttaggcc acgtccagcg gacgcaggtg gtagccgggg cggtgacccc gcccgacgta
    12721 cgcgcggcca cgcgttcgac cagccgggtt cacgaccccc ggctacacca cgccattccg
    12781 cggaagtcgt gcggacgcca gaacgacagc aactggcacc gccgaccccc gcgttcgcag
    12841 agtgaaacgt ctgcagggtg caaaaaaagg cgcgcccatt actggccgcg ccgcgaatgc
    12901 tgaaggaacg ttacgtgact tttataatat ggcagtagtg acttcctagc tcgcactgtt
    12961 tcttaagcaa cgctcgtgcc acgagttgac ggtgacttaa ttcgggattg gcggcgtgtg
    13021 gagccgactg ccgggagacg aacgcccggc gcgcttttgc ttgattaact cgcgaagaag
    13081 cagcttcatt gcaggatgaa gccgatttct ctctcggggc tttcgttgct cctcacgctc
    13141 tcgcttctct cggcgagtcc gagcgatcgg aaaagcggag cgaggattac gaagaacgaa
    13201 gcggaacaca tcgccttgca gcaccatccc ggttcgcggg tgacggcggc catgctggaa
    13261 acggtgcagg gaaaattggt ctggtcgatc gagatcatgc aatcgcagtc gaagctcgtc
    13321 accgcggtcg cggtcgatgc gatgagcggc cagatcgcgg cgccgaagaa agacggccat
    13381 tagccggcca gcctaacgat cagggtttcc cccagatctt ttccaggtag ctcacgcgcc
    13441 cggagccgac atggtaggct tcgtaggcgg cggggttctt gaggtaatat ttttggtggt
    13501 aatcttccgc cggataaaag ggcattgccg gcaaaacctc ggtcgcgatc ggctgatcga
    13561 actttcccga tttggcgagt ttctctttcg aggcctcggc cagctttttc tcgtcgtcgt
    13621 tggcaaaata aatcgcagtt tgataagaca ggccgatgtc gtggaattgt ccgtccgcct
    13681 gggtcgggtt gatatggtgc cagaagacgt cgagcagccg gtcataggaa atctgcgccg
    13741 gatcgtaagt cacttcgatg gcttcgcggt gtttcgtccg gtgcgcggag actgctgtgt
    13801 aattggcgtc ttctttgctt ccgccggtgt atccgacgac ggtcttggtc acacccttga
    13861 cctgatcgaa cggctcctgc atgcaccaga aacagccgcc ggcgaaaatg gcggttttcg
    13921 tctccgcccg agcggtgagc gcagtgccac aaagcgtaat ggcgagtaac gtaaacgaga
    13981 gagatttaaa catggttcct gttacgagag atccggccga tcaattattc caccggcaac
    14041 ttccaccgac cgacccgatg acggatgcaa acagcgtggg agtttcgccc gcttccaggt
    14101 ttgaactgat ccccgcgaat tacttcgcgc cgctcgatct cgcgcaggtt tttccgcgtg
    14161 tggctccatt ggaaatcgat ctcgggtgcg gcgacggggc ttttctcgtg gcgatggcag
    14221 agcgatttcc ggagcgcaat tttctcggcg tcgaacgcat gccgggtcgg gtgcggagcg
    14281 cttgcggccg ggccgcccgc cagggcttga gcaacgtgcg cgtcctgcga gtggaaagtt
    14341 tctatgcggt ggagtacttg ttccctcatg gctcggccgc ggtggcgcat ctcttgtttc
    14401 ccgatccgtg gccgaagaag cggcaccagc gccggcgcat cgtgacgcgg gaatttgtcg
    14461 ccgctgtcca tcgtttgctc gcgccaggag gacaatttcg ggtcgcgacc gaccaggcgg
    14521 attatttcga ttccattcgc gcgtcgctgg ccagcgccgc gtttgccgag gagacgccgg
    14581 atgaagttga cgcgtttccc ctaacgacct ttgaaataca ttttcgcgcg caaggagcgc
    14641 ccattcaccg gcttttgctg cgaaaggcgg cgtgatccat aaatccgctc gcctgccagt
    14701 ggtcgcggaa gaattcgagt ttgaccgcgc cggtttcatc ctggcggaaa agcgtaatgc
    14761 cgcgctcccg caccgtgcga gcccaggtat cgggaattct ttcgcgcgtg ggaaaatcgc
    14821 gggaagtggc gacgatcagc tgtggttgca ccgcgtccag aaattccttt gagccggagc
    14881 ccccggagtg attctggccc ttgatcaaaa tatcgctccg gattccgtca ctagaccgga
    14941 tgagaagcgc atcctccgtt ttccacccgc tgtcggagac gaggagaacg cggaatttct
    15001 ggtcgatcaa cagctgcacg accagcgcct gatcgtccgc cgccttcgcc ttaaaacccg
    15061 tgaccggata caggatgcgg gccgtgaccc ggtgggaaag tgcaaagacg ctgtcccgcg
    15121 ccgcgatttc cctcggcgtc gccagcttag ccagccgggc gaggaacgcc tgatgcacgg
    15181 tggagcgatc acgcgcgccg ttgtcgagca cacgcctggg acggaactca tcgagcactg
    15241 cgcgcgcgcc tccgatgtgc gacgaatcgc cgtggctcaa gaggagacca tcgaggcggt
    15301 cgatgccgtg cgagtgcagg taatcgcgca ggaagcgctc gtaatcgcgg gcgcttcccg
    15361 cgtcgaaaag ccagtccgcg cccggcgcgc gcaggtggac cgccgcgccc gttccggcgt
    15421 cgaggacggt gatttcggtt cgcgcgccgg ttggccagtg cggccattcc acatacgtgt
    15481 gcccggtcgg tagatgcgcg aaggcttgga ccatcgcgag aatgattttt gccaggctcc
    15541 aattcgcgtg gttgaagacc agcgagagcg tggtcgaaaa tgttgcggtc gcaagcgaca
    15601 ggagccccgt tgcgagaata cagaacgcga ccggaacaac ggtgagattc gccaggagtg
    15661 agattggcgt aaccaggtaa aaataccaga tgatcaggag gagcgaaccg atccaggccg
    15721 cggcggaaac agaaacgcct cccgcgatcc agcggtaagc gtgctcgaaa acccgccggc
    15781 gcccgctcac caggctccgc ggcaggaagg gatcggattc gctcggccac aacaggcctc
    15841 ggaaaatcca atcctgcaaa atgagaattg cgccgacgac cgcaaaggaa agctggaatc
    15901 cggaagtgaa gagctggttg ctgtcgaccg cgagaatgag gagcgcggct ccggcgagac
    15961 tgttaagcgc gagcacgggg cgaccaaaaa atatcccgcc taacaagacc gccgccatcg
    16021 tggccgcgcg cacgctcgag acgtggaatc ccgtcacggc cgagtaaaag aagagacacg
    16081 gaataatcag cgccgccgcc gccgcgcgcg gcagacgcac gagcgcggcc aggatccaga
    16141 gcagttgcgc cacgatgccc acgtgcaagc cggcgacggc aaagagatgc agcgttcccg
    16201 tttgttgaaa tggctcctcg atatcgttag gcgtctcgtg tcgaagaccg agcgccattc
    16261 cgttgatcag cgcagtgaca tcgggcgaat cctccagtcc gcggttcagc gtcgtccgca
    16321 tccaggcgcg tgcccgggcg gcgaggcggg gaacgaagcg gccgccgccg gtccggatga
    16381 tcgtgccatc ctccgggtaa cgagcgaaga cgctttggaa aacatcgcgt cgcgccaggt
    16441 agccgcgcag gtcgaattcg ccggggttgc gggccggcgg aatcgtttcg acgctcccgt
    16501 tcacctcgag ttcatcgccc aaacctgggt tgcctttcca gcgcacgcga accgtggcgc
    16561 gggaaggttc tagctggcct ctgaggtcga tcgagtcgag gtgcaaaaga aaagtggtga
    16621 aatcgttagg cgaagctttc ggctcgctcg cgaccgtccc ggtgaccgtg acggacgacg
    16681 cgcgtccgcc aagccgcgcc gccaggtcct tcccgggcgc gtcggtttgc tgaattaggt
    16741 gcaaggcgca gaacgccgcg gcgacgagga gatgactcag ccagcccgcc ggccggaaaa
    16801 gactgacgac cgcgacggcg ccactgcaca ggcagaccag cgcgatcggc acggggaaga
    16861 attccgcgag caagatgccg ccgactgcgg ccagcgccac gccgaccaac ggttgccggg
    16921 gggaatcctg gagattcatc gagcgccggc aggcgattcg ttaggcggga ggatttccct
    16981 ttggcgagcc ggagcgccgg gcggctggcc gtggccgcaa aacaattgct tcacctccgt
    17041 tagtttatat cgttggcccg ctgtgccgac acctttttcg cggaagaagc gaaccaacgt
    17101 catccgggcc ggggcgcaga acggggccgc aaaacaatct gcgacgcgcg tcagcctgaa
    17161 gccggggttg gattggcata agcgcaactt cctcaccgag gttctgattc cctcgctttt
    17221 caccaggcgc tccggcgacc ggacgctgcc gctctttccc aaggtccgtc cgggcgagat
    17281 ttgcattact tggatcggcc acgcctcctt cctgattcag ctcgagggta tgaacgtgct
    17341 gatcgatccc aactggtcga tgtggctgaa ggtgatcaaa cgcctcaagc ggccggggat
    17401 gacgatcgac aacctgccct tgatcgacac tgtcctcgtg acccacgcgc atttcgacca
    17461 tctcgaccgg cgaacgctgc ggcgcgtcgc ggccgatcaa ccgatcgtcg tcccgaccgg
    17521 cgtgggaaat ttggtgcacg atctcggatt caacatcgtg cacgagctcg acgaatggca
    17581 gcaggtgcaa ctcgggccgc tcaaagtgtc gctcacgccg tgtcaccatt ggggcgcgcg
    17641 gatgttgcat gattcccatc gcggcttcgg cggatttgtg atcgaagcga atggtcgctc
    17701 gattttccac tgcggcgaca gcgcttattt ttccggcttc acggaaatcg gaaagcgctt
    17761 cccgattgag atcgcccttc tgccgatcgg cgcttacgac gcgccaagca aacgcgaagt
    17821 gcacatgaat ccagaagaag cggtccgcgc ttttctcgag ctgggcgggc aaaagctcgt
    17881 tccgatgcat tacgggacgt ttcgcctcgg cttcgagccg ttggacgaac cgccggaacg
    17941 attgctctcg agcgcgcgcg cccacggcgt ggaggacaag gtgctggtca tgatcgaagg
    18001 ccgccccgtc gttctctagc cgcgcgcctc cctcgcttga acgagaaaac tccgcgccgc
    18061 gccgatctct tcggggccgc ggcggcgggc gcactcgtgc tcgccttctg cggtctgctg
    18121 ctctggcaca atccgcttct tttttggaac gacgatttcg agatttcgat tctgccggtt
    18181 ttcgccgatg tcgcgcggag ctggtcgtta gggcaggtcc ctttgctcag cccgtattcg
    18241 tgggtctgcg gcaacctcgc gggcgaattt cagtacggga ttttctccct cttcgtgaat
    18301 gcggccgtcg tcctgatctg gaaatttccg ctcgcctttg cgcagcaggc cgcggccctt
    18361 tcgctcgcgc atttgttcgt ccttgcagcg ggcgcctttc tcctcgggcg gggccggaat
    18421 ctttctcccc cgcttgcggc gatggtggcc ctggtcgcgg cgctcaatgg ctggatcatt
    18481 tgctgggggg cgtcggattg gttcggcgcg ttggctgcgt tcgcctggct gccctgggcc
    18541 tggtggggaa tggaacgggc gctcgatgag tcccggacga agttccgatt tctctggccg
    18601 gcaccgttcg tttacctgct catcaccggc ggatttccct acacgatttt gatgctcggg
    18661 ctgctcgcgc tttggctgac ggccaaatcg ttaggcgagg cgcgcccgct ttcctcgctc
    18721 gggccgctcg ccctcggtct ggcgcttggc ggcggctttt ccgcgccggc ctggctcgcg
    18781 cttttcgatt acgtccacgg ctcggcccgg gccgcgcagg aaaatcccgg cgtccattgg
    18841 caatggctcg tgcccccggc cgccctcccg ggtttgatct ggccgccgtg gactgtcctg
    18901 tggaccgact tttggactcg tccggtcccg catgctgcga tcgagctagc ctgcggcacg
    18961 gccccgcccc tcgtgctcgg cgccgccctc gtttttttgc gcggtcgatt cgttaggcga
    19021 atgaaatggg aactgggatt actcgtcctc gtcctgctca ttgcgatgct cccgagcgcg
    19081 aacgtttttc gctggagctt tcgctggctg ccattcttcc acctcgtgct ggggttgctg
    19141 gcagccgaag cgcttgccca gtttcgtttg gcgaccgcct ggcgctggct ttttgtactc
    19201 gtcgtctttg ggtcgctcct cgcaacctat ctcgtccttc caacccaccc ggcggttccg
    19261 cgatatcact ttgcctcccg cctgacgagt ccgcagccac tcgatccagc ccgcctttat
    19321 ctgagcgtct atcccccgcc ggagacgact taccgagtgg aaaatcatcc gtcgcccgtc
    19381 gggcaggtga cgcggcccgg cagtacttcc atgtgggccg gtctgcattt cgtgaatggc
    19441 tacagcccga tttgcggtgc gggagtgggc cgcgctttcg ccttctccac ccatggggag
    19501 atcgatccgg ggatggcgga ttacctgctt ggcgacgagg ccgggcgcga cgggctgctt
    19561 gcgctcctcg gagtcgacgg aatcgtcgtg gcccacgaga tcgccgccgc gccccagccg
    19621 gccacggaat ggacgctcgc gcgcgaggaa gcggaagggt gcatctatca gcgcgtgggc
    19681 gggccgctgc cgcgggtccg ttccgtcgct tcgctgcctt ccgcgcctaa cgagaggctg
    19741 gcgcgcgcga cgattgggtt gcgcgccgac acgcgccagg aaatcgtcgc cgaggtggaa
    19801 gtgccggccg gcgctgctcc ggcgctgctt gttttctcgc ggccctattt tgacggctat
    19861 cgcgcgaccg tgaacggaaa aaagatcgcg atcggttcct atcgtggcct catcccggta
    19921 atcaaactcg cgcccgggac gcagggtcga ctggtggtga tctatcggcc atggtggctg
    19981 ctcgcgggcg gaacaattgc cgccgccagc ttgctcgtga tgctcagcgc gctcgccctg
    20041 gccgcgtttt ttggagcggt ccgtcccgct caataaacat cgcgcttgta gcgcttatcg
    20101 ttcttcagct tttcgacgta ttcgctcgcg gcctcgacac tcttgccgcc ctgtcgctgc
    20161 acgatctggc gcagggcagc gtctacatcc ttggccatgc gcttggcatc gccgcaaacg
    20221 tagaagcagg cgccttcgtc gagccatttc cagagttcct ccgctgcttc gagcatacgg
    20281 tcctgcacat aaatcttgtg cgcctggtcg cgcgagaacg cggtgtcgat tcgggtgaga
    20341 aatccgtcgg cgatgaaacg ctcgaagtcc tcctgataat agtagtcgct cttctcatgc
    20401 tgcgcgccga agaaaagcca cgtcctgccg cgcgctccga tcgcctgccg ttcctgcaaa
    20461 aaggcgcgga agggcgccac gcccgtgccg ggaccgacca tgatgagcgg cgcgtcgcgg
    20521 tcctcgggca ggcggaattt cgagacggcc ggaacaatcg gcgcgggcga atccgcgcag
    20581 cgctcggcga ggaaggtgga acagacgccc ttacgcagcc ggccatggct tgtgtaggtc
    20641 acgacatcga caatgaagtg ggtcgcctcg gggaaaactt tgagactcga agcgatggaa
    20701 taaagccgcg gctgcaactt cggcagcaga gcgacgaatt cctgtggctc gaatttgacc
    20761 gacgggtgtt cgaccaggaa atcgattact tccatgcccc agagataggt gtcgagatcc
    20821 tgtttccgct tcggctccag cagctcgttc aagagcgggg cggcggaggc gcgttgtgca
    20881 atcgccttca ggaatttcgg ggtcggttgg gtgatgccgt gatcgcggag taacgcttcg
    20941 cgcagcgtcg ttggcgcgcc tcggttgccc gcgatttttt cctcgcccgt ggcggcgagc
    21001 gcttccaaaa tttcagcgac cagcccggga tcgttggtcg ggtagaccgc catcgagtcg
    21061 ccgacttcgt aggtcagccc ggagtcgccg aacgagatct cgaaatggcg aatgtctttt
    21121 tccgaatcgg gactgttcaa gcggcggttg acaagcaggc ggcccgggaa gggatttttc
    21181 cgggaataag tttcggacgc agcgggctgg tcgttaggcg agacggacgg gtttgcggtt
    21241 tcaggcacgg ccaaccttct agcgtggtcg cggcgatcgg caacgttaaa ctattcgtgc
    21301 catcgtcggg gcccgtccct ggccgcggtt gtgcgcgagg acgcgccggt gaaaggtccc
    21361 tccgccatgc ctcgctcgct gcgccgtcga aaaagcgatt atctgctgat cgacatcagc
    21421 aactccttta ccaaacttgc ctttgcttcg tggaaaaaac tgggcggaaa agagcggatc
    21481 gcgaccgatc gattaagcgt ggccgcgctc ggaaacttga tccggcaacg agcggtcgag
    21541 cagattgtcg tctcttccgt ggtgccgggg aagaatgcgc tgatccgtcg ggcggcgggg
    21601 aggattccgg taaccttcgt gagcgcaaaa tgcgagcttg gggtcggcgt cgattatccg
    21661 gctccggaat caatcggcgc cgaccggctg gcgaacgcag cggcggtcgc ggcgctcttc
    21721 ggttcccccg ccgtggtagt ggactttggt acagccgtga cgtttgatgt ggtctcggcg
    21781 gaccgctctt acatcggcgg cgtgatcgcg cccggtctcg aagcgatgac cacttatctg
    21841 tataagagga ccgcgctgtt gccgaagctc gtcctcgccg aaccgcggcg cgcggtcggc
    21901 cgctcgacga aagccgccat gatgtcgggc gcgattttcg gctatcgtgg cttggtgaag
    21961 gagattctgg tgaaaattgg cacggaggcc tttggccacc gcaaagcgca agtcgtcgcc
    22021 accgggggct atgcgcaatt gattgcggcg cagctgccgg agatcgacgc ggtgcgcccg
    22081 cacttgacct tggaaggttt gcgcatcatt ggaaacctga atcgttaggc aagaaaatgc
    22141 cgtgatgaac aagacggcca ttggcatcga tttcggcggc acgaccgtga aatcgggcgt
    22201 ggtgcgcgaa gggaaattgc tcgcgcgcct gccgcctctc cagacccacg atcataccgg
    22261 ccacgattcc ttgctcgcgg ccatccgcgc ggcgatcgaa gaactacggc ggcaacatcc
    22321 ggacgtggcg gcggttggcg cggggttgcc ggggctggtt gacagcatca atggccgcgt
    22381 ctggcagttg tccaatgtgc cgggctggga ggatgttgcg ctcaccgcct tgctcgagga
    22441 gtggaccgga ttgcccgcgg cgatcgacaa cgacgcgaac gcgatggcat acgcggagtg
    22501 gctttttggc gccgggcgcg agggcgtcaa cgtcgtttgt gtgacgttag gcacgggcgt
    22561 cggcggcggg ttgatcctcg accgcaaact ttatcgcggc agccgactgg gggcgggtga
    22621 gatcggccag atgacgatcg atccgaaggg cgttccgggg cagtacggca attttggcgc
    22681 gctcgagaag tatgtcggca acgcgcagat cgcggagcgc gcccagaccc tttaccggga
    22741 agccggcctc acgaaaacaa tcgaggaata ttcgccgctc gccttggaaa aagcagcccg
    22801 ggcgggcgac ccggtagcag tgaaggtctg ggaggagatc ggtttccatc ttgggatcac
    22861 actgtgcgac attgtctggt tgctgaaccc ggatcggatc gtgatcggag gcggcgtggg
    22921 gcgggccggg gaatatgttt tcgggccgat tcgccgcgtc atcgaggagc ggacgatgaa
    22981 gatctttcac gagggcctga cgatcgttcc ggcggcactg ggcaatgacg cgggcatgat
    23041 tggcagcgcc gcgctcgcgc tcgccaaggc gaccgcgcct aacgaggtct aacgactatc
    23101 ttccggcgga gcaacctctc gccgtcgaac tcgaaccagg cgcgcaaatg cgcgccgcgc
    23161 aggaggagcg ggagccagtg ccggtcgtcc gcccacatct cgtcgtaggg gatggcgtcg
    23221 agcggcgtcc agagcggcag cgcttcgtcg gtctcgcgcg gcttgccggt gaaatcatgg
    23281 gcgagaaaga cggtgcaatg caggctgtag ccgtcgcgga actgaaaatg caattcgccg
    23341 cgttgctcgg ccccgagcgg tttgataccg acttcctcat aggtttcgcg gagcgccgac
    23401 gccagcggcg tctcgcctgg atcaaccttg ccacccggcc cattaatttt gccggcgccg
    23461 aacccgcgtt tcttgcggat gaggagaatc atttcctccc gcacgatgaa acagaggctg
    23521 gcgcgttgcc gcggttgcca ggttgcccag tcgatcatgt cgttcgatga gttttagatg
    23581 ccgtcaggct cgatcgacgc gagcttgttg cgatcctgat gaagatctcg gaaagcggtc
    23641 tcattttttt gctcgtgttg agttcgttcg cggcgaatgt cgtcgcgccc accaagtcgg
    23701 aactcgaggc gatgtatgcc gcggcggccc gcgaactgaa cgccggccat tacagcgaag
    23761 cgctcaagga actcgatgcg attgacgcgc gccagcccga tatggcggcc gcgcagaatc
    23821 ttcgcggcat cgctttgatg cgccaggcgg aatatcgcaa ggcggagacg gcgctgcacc
    23881 gggcactgga gattgacccg gagttctggg aggcgagctt caacctggcg gaagtctcgt
    23941 tcctgatgca aaactggacg gaagcgcgcc gtcgattcca ggcgctgctg gccgagggaa
    24001 ccgaacaggt gcagggcgcg acgagggatc tcattcagtt caagatcctg ttgactttcc
    24061 tgcttgaggg caaggagaag atgggcgccg cgaccctgga gaaactgcag gcatcacacg
    24121 acagcccggt ggattattat gtcaaggccg cgctcgcgct gcgccagaaa gaccagcaga
    24181 atgcaaagat ctcgatggcg gccgcggaga aggaattttc tccgcagttg aacaagcttt
    24241 tcgccgagtc gttctatgaa gtgggctggc tgcaaaagcc ggacggcgcg actcccgtcg
    24301 ccttggaaat cggctcgccc gccgagcgcg tcgcgcaggt gcaggagaac tttgggaagg
    24361 cggagcgcgc ttatcgccag cgggactttg cgagcgcatt gcggttgctc gatcaaatcg
    24421 acgcgactgc tccgaaccag gcagtggcct acaatctgcg gggcgagatt ttgctggaag
    24481 agggaaaggc ggacgcggcg gaagctgcct tgcgcatagc gctggcggcc gatccccagt
    24541 tgcaggaagc gcgctttaac ctggcgcggg ttccgttcaa gaagggggat tacaagacgg
    24601 cgcgccagga gttggaagcg ctcctcggcg cgacctcagg cggaaaacag caacagcaaa
    24661 tggaacaact ggtccgttat cagattttcc tgacgctgct cctggagggc cgggagagtg
    24721 ccgcgcaaaa agcgatggaa gaattcaaaa tgatggacga cacgccggcg ctctactatg
    24781 cgcaagcggc ctgggccttt cagcatggga acgcgatgca gggaaacaat tgggtggcga
    24841 atgcgaagaa tctctattct tcagaactca atcgctcgtt tgcggcgtcc ttcgctgatc
    24901 ttggctggct caaaaattcg gaggcgcaac cagcgcaaac ccagtcctct ttagccgagg
    24961 cggaggtgtc gccgcgagcg gagccgccgc cgacacgacc ccgaaacgag aacaatccaa
    25021 tgccggttcc ggtggcggcg gagagtccga ccgaccagcc aatagcgacc acagaatcca
    25081 cggcgacgcc tacgccgccg gttgcgctgg cgggtatcgt cgccgcgagt ccgactctga
    25141 ctctgaccgc gacgccgaac aacgttgcga gcgtggaggc gaagcaaccc cgtgaagagc
    25201 aagcttcctc ggcgggaaca aaaagaaggc gctctgctgg cgcaaagtcg aagcgtcatg
    25261 cttcggtcgc tgcgccaagt ccaacgccca tggcgcaacc gacgcgccag aatttcggcg
    25321 ataaagtggc gcgactgttt ctctaccctt tccggcatcg aagcgaacag ggttccactc
    25381 ctacgcctgc tggatccgac gaggcagcgg ccgcgagcgc caactccaat ccgcctgccc
    25441 ggagccagcc aaagaattaa gacttgtcat ctcgcacccg tgacggtttg atgtttcctg
    25501 tgatgaaaat ccgaaggctc gccgcgattt ccctcctcgt tctgggttcg ttcgcgtcca
    25561 ccggtctggc cgcggaaaaa tcgagcctcg aagcgatgta cgaccgggcc ttcacggcgt
    25621 ttgacgcggc gcgttacgac gaggcgttaa aggccctcga cgcgatcgac gcgcgccaac
    25681 ccgatctggc cgagtctttt aatttacgcg gcgtggtctt gatgcggcag ggcaagtacg
    25741 acaaagcgga ggtggcgttg cgcaaagcgc tctccatcga gccgaagttt tggaacgcga
    25801 gtttcaatct cgcggagatc ccttttctga aaaaggactg ggcggcagcg cgcacccgtt
    25861 tcgaggggct gatgtcgggc gagaacagcg ggatgcaacc ggagacgagc cagctcatcc
    25921 aatacaaaat cctgcttacc tttgtcctcg agggaaagga gaacacggtc gattggatcc
    25981 tgaacaagtt cgaactttcc aaagacagcc cggcgctcta ttactccaat gccgcaatcg
    26041 ccctcgaaca caaaaaccag gcggaggcga aggagtggat ggatgcggcc gagaaaaaat
    26101 accccgggcc gctgaacaaa ctttacgccg aatcgtttta tgaaatcggc tggctgcaaa
    26161 aacccagcgg cgaaagccgg cccgcgctcg agattacctc caccgccgat cgcgtcgcgc
    26221 ggatgcaggc ggatgcgaaa gccaatttcg agaaagccga acgggccttt cagcagcggg
    26281 acttcgacgg cgcgctcaag cttctcgatc tcgcgaccgc gggcgcgcct aacgacgctg
    26341 cttcggacaa tctgcgcggc gagatcctga tggagcagaa gaaattcgat gaagcggagg
    26401 tcgtcctccg ccgggccttc gcgaccgatc cgaaattccg cgaagcgcaa tacaacctcg
    26461 cgcaaattcc tttcaaaaag ggcgaatacg ccaaggctcg cgaccggctc gaggcgcttt
    26521 ttggcgagac gcccggagac gaaaaaaatc aggccgcgca gctcatcaaa tacaagattt
    26581 tcctgaccct gctgctcgag ggcaaggaga gcgaagcgca gcagttgatg gagcagttta
    26641 aatttacggg cgatacgccg gccctctatt acgcccaggc ggcctgggag tacaaacacg
    26701 ggcatgaaga tcaagggagc gattgggtca cctccgcacg aaagatctat tcgcccgcgc
    26761 tcaacatcgt ttttgccgat accttttatg atctcggctg gttaaaaaac ccgaccgttg
    26821 cgacagcgcc gccgccgact tcggcactgg cccaggcgga cgcttcgccg ctcgttgaac
    26881 ccacgccggc catgcgtctg ggccagatgg agtcgcgacc cgtaccagac ctgaccgggg
    26941 atggcatggc gaaatcaacc gacgtggagg caaaggaacc agctgcgccg acgccggctg
    27001 cgactgggct cgaaacgact gcgcccattg ccaccgggcc ggttgcgacc gcggcacaga
    27061 cgaccgcgcc aactgtgacc gcggctcaga cgactgcgcc gattgcgacc gcgccgacgg
    27121 cgaccgggcc cgagacgact ccgggcgcga tggccgcggc ggtggccgcg gcgactccgt
    27181 tggctacggc ggtcccggct gtttcgccgg ccagcaaacc agccacttct ccgatcgctg
    27241 tcgcgagttc tgcagtgaat gtcgcggcgc cctcactggc tgcgacgcag accggtgcct
    27301 cgtcgcggtc gaccttcggt gaaatgatcg atcgggtatc gagcccgctc agtccgctgg
    27361 tcggcgttct gttgctggca ggaattgtgc ttctcctttg gttagttatg caacaaatcc
    27421 gtcggtatct cgccaataat tcacttttca agtcatcgac gccattgacc gagccgccct
    27481 tcgctggcga tgaaccgatg gtgagtgacg aacataggat ttcgaacgac attttatcga
    27541 gcggtccgcc caaggtctcg ctccaattaa aagcgagcga gccttccgtc cgcgcggccg
    27601 ttctgccgtc gggggcgatc accgcccgcg gcgccgttcc cggggttccg gagccattca
    27661 acctccagcc taacgaactg gagggttcga tcgcggaggt gatgcccgcg ccggtttcgg
    27721 ccagacctcc ggtggagcca ggcccgacga tcgaggaagt tcttcccgcc gcggaagcga
    27781 agattccggc cgaagccgaa gcggtgctgc gcgatcagga agaagtttcc ctgcccgccg
    27841 cgctcgaagt gccggcggct tctcttgcgt cgcaagccgc cgaaccggcc gctccggatg
    27901 aacccgtcgc tccggttgaa cccgtacccg cgcaaccagt ggctgagaaa tcggcggcgg
    27961 aggaagccgc gatgatcgca ccggagatca tcctcgagcc tgccatttcg gaattcgccg
    28021 gtcacgcctg ggagaaaatc gctcccgaat tggagcccgt ggccagcaca ccgatcgagg
    28081 aacccgcttt gccgcacgaa gaacctatcg gccagggaca gccgattcca caactcacga
    28141 ccgcgctcgc ggcggaagcc gtcacggccg aggtcactcc gcccacgccg gaaactccac
    28201 ttgttttggt cgaacccgaa gccgcggtgc cccctccgat tgccgaatgg caagcgccgc
    28261 agtcttcgat tgaaactccg tcatttgctt ctaagatcat caccaccgaa cccatccgac
    28321 tacagccaac tacacccgtt atcatgcctg aaaccaccat cactcccgct cctgccttcc
    28381 gtgccgctac accgaccatg tctgtccaac agccgctcgg cgggatgcac accgcggtgc
    28441 aattgacgtt ctctttggag atcgcctcga tgcagctcac gcccaccttt aagatgagcg
    28501 gcctgcaatt gaagccgatt tcgaaagtcg tctcgatgcg cctcgcgcct tcgcaggatc
    28561 cgcaaccacc gatgaacctg caggtcacat tcgaggtcgc aaagatcgaa ctctccggtg
    28621 gcagcatcgg caccgtccgt ctggcgccct cgttgcagga gaagccggcc gctctttcct
    28681 cgccttcctt cgccatctcg ggtctagagc tggtcgcggg cgcgggcacg gctccagtgc
    28741 aactgactcc ttcccaccag gaacaggctt cggtgcttct gaccgcgcat ttccaaattg
    28801 cggcgatcga gttttcgccg ctcttcgaaa tcgcggcgat cgtgctcacc tccacctcca
    28861 agcacgtctc catgcaattg ccgggttcgg gaccgagctc gatcgataac gcaccggtct
    28921 tccagatcga gaacgtccaa ttgggtgcgg gcgccgatct gaacacgatt caggtcaccc
    28981 cgatcggtac cggccggttg acctaacgag cgacgaccca ctgatcttta gcctggagcc
    29041 ggggcgttgt ccccggctct tttgctattt aaaactcgtc cagttgagcg agcgcaactg
    29101 cttgggcaga aactcgccgt ctcgcctcac gagctggcca tcaaaataaa tctcccctcc
    29161 gccgtagtcc ttccgttgga tgctgaccat gtcccaatgg acctggctgc ggttgccgtt
    29221 gtctgcctcc tggtaggcct gtcccggcgt aaagtggaac gagccagcga ttttttcatc
    29281 gaaaaggatg tcgcgcatcg gatgcaggac gtagggattg cagcctaacg aaaactcacc
    29341 gatgtaacgc gcgcccggat ccgaatcgag caccgcattg agctttttcg tctggttgct
    29401 ggtcgcctcg acaatcttcc cgtcgcgaaa atcgagccgg atattatcga agccggtgct
    29461 ttgataaatg ctcggcgtgt tgaacgtcac gtgcccctgg acggaatcct tcaccgggca
    29521 gctgaagact tcgccatcgg gaatgttgcg gtctccacca cagatgatgg cggggatgcc
    29581 cttcatgctg aaacgcaaat ccgttccggg tcctttgatt tccacccggt cggttttttc
    29641 catcagcgtc ttgagcgctt tcatcccggg ctgcaacttg cggtaatcga gcgtgcaaac
    29701 cttgaaaaag aagtcctcga aaccctcggt gctcatcccg gcgagctgcg ccatggccgg
    29761 ggtcggccag cggagcacca cccactttgt tttctgcacc cgctggtcct gcaacgggcg
    29821 cagctttttg gcgaccagtt tcatccgatc gatcggaaca tctgcgagct cgctgatatt
    29881 attgctcccg cggaccgcga tataggcgtc catctttttc atccgggcca gctcgtgcgc
    29941 ggctgtgagg tcgagttggc gcgcgtcgat cccgagcgcg agctcccggc tgatccgggc
    30001 ccgctgcagc tggacgaacg gaatcgcccc cgctttccgg gccgcgcgaa tgagcgcgat
    30061 cgtcatttca tcaggcgcgt cgaaggattc gatcagtacc ttttcgtggc ttttaagggc
    30121 agtggaataa ttaaccagca gggaggcgag cttgtcgaaa cgggcatctt gcatagatcg
    30181 ctatcattcc atcgtgatcg agggatgcaa acgtgcgccg atcacggatc ttcctcgtga
    30241 tcgcgatcgt gatcgacgag gatcgagcgc gagccggagc acggggatgg ttcgttaggg
    30301 tgaaaattcc agacgggcgg caaaggcgcc gtcgatcaaa tcccggaaag gcaagctctc
    30361 ctctcgcttc gtgagccgaa aggcgggctg agcgcgcagg aacgcggcga ccacttcgtc
    30421 gttctcctcc gactccaggc tgcaggtgct gtagaccaac gaaccacccg gcctgaggag
    30481 cggcgcgatc gcccggagaa tctgcccctg ccgccctggc atccgcacaa aatcttccgg
    30541 ccgcagccgc cagcggagat caacccggcg ccgcaacacg cctgtgttcg tgcagggcgc
    30601 atcgacaaga attttgtcga aggactgcct cgcgagcgcg gcctcgcgga gggaattttc
    30661 gtccgagcaa tcgcagcgca ccgttcgtgt gttggtgacc tccagcctaa cgagattgtt
    30721 gcgcaaccgt tccaaccgga cctcgtcccg atcggccgcc acgagcgcgc cttcgttctg
    30781 catcatctcc gccagataag cggactttcc gcccggcgcg gcgcaggcgt cgagcacgct
    30841 ctccccgggc gccggacgca acatctcgca cgccagcgcc gtgctcgggt cctgaaggta
    30901 acaatccccg ttgcgcagtg cgtcggccgg gccaggcaaa ccgacaaaat tcgagtgtcc
    30961 cggcaacaag aaactgccgg cataacggcg caggaattcc gccgccgtcg tcttgagctc
    31021 gttgattcgc gcgtaaaccg gcgcgggccg gttattccac cggcagagct cgaacgtggc
    31081 cgcgctcccg aattggcgcg accatttggc gatcagaaat tccggcgcgg aaaagcgaat
    31141 ctggagcggc tgttcctcgg cctgttgctt gagggccatt ctctcgcgca acgcgcgccg
    31201 cagaatcgcg ttgatcaacg gccgcgtccg tggcctggcc agctcaacgg tttcgaaaac
    31261 ggaggcgtgg gccgcggttt cgatgaggag aatctggtag aggccgagcc gcagaatgtc
    31321 gcgcgcgtcc gcctcgagtg gggccggccg gagttgggcg atccaaaagt cgagaagcgt
    31381 gaggttgcgc agcgcgccgt agaaaagctc gagggcaaaa gcgcgatcgg aaacgccgag
    31441 cgcgctttgg gcaaagattt ccgcgacaat ctgatcggcg aattctgcgc ccttgcgcca
    31501 gcgggccagc gctttgagag cgagcgagcg tccggattct ttcatggttc gcgctggcgg
    31561 gcgacacgcc cgcctctgca ccccggggat tcgacgcttc gttaggctgg cctggcctgc
    31621 ggtcgcccga tcgaggtgta gtggaagccg agtttccgca tcgtctccgg gtcgtacaaa
    31681 ttccggccgt cgaaaaccat cggtgtgtgc attttttcac gcaccgtggc gagatcgaca
    31741 ttggcaaact ccgcccactc cgtcgcgatg atcagcgcct ccgcgtcctc gatcgcttcc
    31801 attgcggtgt tcgtgaggcg cgcgcccgcg atcgatttca cggcgtgcgc tttttccatt
    31861 cccttgggat cgtaggccgt cacctgcgcc ccttcgcgca gcatctcggc caccagctcg
    31921 atcgcgacgg atgaacgaat gtcgtccgtg tcgggtttga aggtcagtcc ccagacggcg
    31981 atttttttgt cgcgcaaaac ccagagcgcg tcccgcacca ccttgaggaa acgctccttc
    32041 tgcatcgcgt tgatccgctg cacttccttg agcaggttga acggcacgcc gagctgctcg
    32101 ctaatcatga taaaggcggc gatgtccttc gggaaacagg agccgccgta gccgatcccg
    32161 gcgttgagga aattccgccc gatgcgtcga tccatcccga tgccgtcggc cactttttcg
    32221 acatccgctc cgctcgcttc gcaaatcgca gagacggcgt tgatgtagga aatcttgagg
    32281 gcgagaaagg aattggccgc gtgcttgatc aactccgcgc tgttgatgtc ggtcacgagc
    32341 atcggcgcca tgaagggctc gtaaaccttt ttcatcaggt cgatcgcgcg ctcgctttgc
    32401 gcgccgatca cgatccggtc tggcttcatc aggtccgcga ccgcgcagcc ttcgcgcagg
    32461 aactccgggt tgcttacgac gtcgaactcg gcgccgtgtt tgttatagcg cttgatcgat
    32521 tcggcgacct tctccccggt cttcaccggc acggtgctct tgtccacgat cacccggtaa
    32581 ccggtgagca cgccggcgat ttcgcgcgcg actttctcga tgaagctcag gtcaacactg
    32641 ccgtccgcat cctggggagt gggcacggcg atgaaaacga cctgcgcgtt atcgacccct
    32701 tccttgatgc tgctggtgaa gcgcagccgc tgcgcggtca cgttgcgatg cacgatttct
    32761 tcgaggcccg gttcgtagat cggcaccttg ccgtgctgga gcgactcgac tttgctttcg
    32821 tcgttatcga cgcagatcac gttgtgaccg acgtcggcga agcaggcgcc ggtgacgagg
    32881 ccgacgtatc cggaaccgat gatggagaga ttcatgggaa atttatgatt gctgattgct
    32941 gacttggcag gggacaaacc gccgcctgat ttcaatcaga aatcagaaat cggcaatcag
    33001 caatttccta ctgggtctga ttcgtccccg ccggatcgaa attcaaatcg agcccaagtt
    33061 tgggaaacgc cttcagggtg aaggtgagga gaacgccgta ttccttgacc ccgccattat
    33121 tctggatgat cgcccctaag gaggccaccc agctggtcag gtcgcggtag agcgagaagc
    33181 gctgctgttc gaagatgttg gtggccccct cgtactgctc gaggatgccg aacccccagt
    33241 tgtcgtcgat ccggtaatag ccgccgaagg tgaagaggct gctgttgagg aagaacggat
    33301 tattgttcag gtagcgatgc ccgagcgtaa tgacggtgtt cgcggtcggc ttgaaattga
    33361 tgttcgtatc cacctcggtg aatccattgg cgagcagcgg gagctgcgag tcgatgacga
    33421 gcgtcgccca cggcaccggc gtaaaggtca gcttgttgaa gacgttcgaa tagtcgcctt
    33481 tttcgaaggg gtcgtggaag ttgacgtcga cgaaggtctc cagctcgagc cagctcacgg
    33541 tcaggtcgtc gcggcgggtt tgcaggcggt tgcgcacgcc gacgcgcgcg atcgaccaat
    33601 tatcgagcgc gtcgatcgag gtgtattgcg ggaagtcgat cgggttgagt ttggtcgaag
    33661 gctgaacgcg gtcgaattgc agaatctcgg cgggatccac gttaggcgaa gagacatagg
    33721 aatagtccac aaacggctga atgatgtgcc gcaagccatc gagccccagc gcgcgggttt
    33781 gcacgccgtc ccaggcctgt gaaatcttga acgagccctc gatcccggcg ttgaaaaccg
    33841 agcggaaaac cccgcccgca gtgtgcaacg gcggctcagt ggtgggatca gccagcggga
    33901 attcgggcgc gttcgggtcg ggattaattg ggagaggcga caaggagagg ttgcgtgtct
    33961 cactatagta ggtctcgcga aagccgaccc gcggcacgat cgcgagccac ccgaaatagg
    34021 tgttcggata ggtcagctga tggaaggaat cgatccgcaa ggcactgtaa tcttgaaaga
    34081 gcgatccggc cgcgaaattc cggttcagct cgcccacgct ggtcgcgccg tcgtagaaaa
    34141 tcgggccgtt gaagagaggg tggcgcgtga tgtcgagggc gatctccggc aggcgctcgg
    34201 tggtgtcaaa aaaagtgttg agttgaaatc gcgcgtcgac ggtgagggta taccacggac
    34261 tggtttttgt gacggcgacg acattgtccg gctgcgggtt gatccgaaac tccgccggga
    34321 aaaaatcctg gaggagaaac gcgtcgctta gtttggtgat atcgaccgtc gcctcaatgt
    34381 cgtcggtaat gtgagtgcga tcctggatgg acaggcggta gcggccggtc ggagtgcctt
    34441 cccgggggag cgcggtgcga ttcagattgg gattctgatc ctgcaaataa taggagatga
    34501 tcttcgcgac gctgctgtcg ccctttccgt agcgaatttc cgaatcaaac ccaatcgcgg
    34561 gtccgcggcg ggtgcgataa tcgagcttca ccgtgctcgt gatatggtcg ccgatcggga
    34621 aagtgacctg gccgagaagc gcaaatcccc acgagctgag ataggcgggt gcaatcgagt
    34681 agctgaagga acggtcgagc gattggtaga gatagggcca ccagaagacc ggtatctggc
    34741 cgacgtaaaa cgtcacgctc ttgaaaatga cccgatcatt ctcatagatc cgcacactct
    34801 gggcgctcat atgaaagtcg ggttgtgcgg cgtcgtgtgt cgtgaaggtg ccgttcttga
    34861 ccaggtattc ctggtccgag tcgctgttca ccgtcttccc ggagatcagg tagggaaagg
    34921 tgccggagcg catgctgtcg gcgttgatcg ctttggtgtc gagattgtag gtcgcctgct
    34981 ccgcgagata gagcgaaacg ccacggtaaa tgcgcacatg gcctttgaga aagaccgtcc
    35041 gcttggcctt atcgtactgc gcgtagtcgg aataaatatc ggtgtcgccg aaacggatcg
    35101 agacgttgtc gtgcgccgtg gcaatgccgt tctcgtagat catgcggccg gtggcgttga
    35161 tctcgacggg ctgcgtcccg ggcccttcca aatcctccgc agccgcgcga ctgacggcga
    35221 gcacgaagag gagaagaaag accggcttac gcattgcgga cgccgaaggt aacggtgcca
    35281 cgaagcggtt gcaaagtgaa aacccttttg ccattccggc tccagctcgt gaatcgtaat
    35341 cgaaacgatc gatcgcaagc acgacgagga tcagcgacgc agcgcgagtc cggcgacttg
    35401 ttgattcagt ccgcaagttg
//
LOCUS       DATMNS010000113        14793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3302194, whole genome shotgun sequence.
ACCESSION   DATMNS010000113 DATMNS010000000
VERSION     DATMNS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 14793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14793
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3302194"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..416)
                     /locus_tag="VNW28_04145"
     CDS             complement(99..416)
                     /locus_tag="VNW28_04145"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HXA09146.1"
                     /translation="MVHHIGLYKLKPGVTPEETEEMMMNARMQLLKIDEVLSIKCGKR
                     VDPKIPWPFFIAIDFESMDKQAIFREDPIYIKFVEEVIKPKTSEYLGLDYEMDPGKDV
                     NLS"
     gene            complement(527..898)
                     /locus_tag="VNW28_04150"
     CDS             complement(527..898)
                     /locus_tag="VNW28_04150"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXA09147.1"
                     /translation="MIREYIPILLQALVAVGFAAGALIISVLLGKSGLRTQTKDSAYE
                     CGMVPQGEAQPRFSVKFYLVAMLFILFDLEIVFMYPWAVVYREAVAHSHGIFWSMLSF
                     VSILMVGYVYALKKGAFDWKK"
     gene            complement(947..1270)
                     /locus_tag="VNW28_04155"
     CDS             complement(947..1270)
                     /locus_tag="VNW28_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09148.1"
                     /translation="MAILKKNAFSRRVPDLVTEELVAVLSRRSSFEFKQLFEILHENL
                     RARNAASGGEEMLRLRAYEKLQNLVARGMVRKTGKKYKGLPSALAKALLPPNGHIPTA
                     AATSR"
     gene            complement(1393..3192)
                     /locus_tag="VNW28_04160"
     CDS             complement(1393..3192)
                     /locus_tag="VNW28_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09149.1"
                     /translation="MKKLAAVIVIALVVAGAVWVILRVQLAQRLATVPELLPKTTLAL
                     ALVPDFQQTRKQWHESDIYQIWREPSVQACLQEPLARLPQDGGGRKTLEEFLQLGPTH
                     GFIALASLESNEPKLIGGFHFEPAPEEARKFIEQRQAEWFPKTGETKRETIVYQQHKI
                     ETVSVSHFVFASVYDNHWFFGSNDPALLEALLDRVDHRRGEKEASLGENDSFSAALKH
                     LPVEYGGMIFLDPRPFVEKLTPVIALTGQSLVTNQLAQLKQVRSMAAALGFDHGKMRE
                     TDFVGMPRVGAEEKLARRSLAAAGANTFLYSASQIHWPENASSPSTPAAGRMPAILQQ
                     LMNAMTARGMALNDLREAFGEELEIVGDWPTDARWPTLQATLPVKDAARVRKIAEALT
                     SVEIAGAAWTRREKDGAIVYSLQPLGGLIPLSPAIAVSDKMMCAGSDAAAVEAAVATV
                     ARPAGELEKSALFRDAANQVPEPDSGFNYVDTRLLFERADAAVRPLLLMSATFYPALG
                     KNIDPAKFPPAEAITKHLSPIVMSERYETDGYVTESVGPVTFRQATIGLAGLIGGAFI
                     YLQEGLKRGASSQPNPGDPLPSAATPTPTASPF"
     gene            3275..4444
                     /locus_tag="VNW28_04165"
     CDS             3275..4444
                     /locus_tag="VNW28_04165"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HXA09150.1"
                     /translation="MEACAGSRLILGEFRESAVVAAAEQALAECGGQPTCGFLFLSPS
                     WLPQIPEVLDLVRLHGRIPLLLGSVGAGLIGRSQEAEGVAGMSLLLVRLPETKLTPVL
                     ISSEQIEESTGPAYWQMETGIGPDDVDSWIVLANPYAPDLERWLAEWNVAFPDAPSIG
                     GLSSGAGDGGEKALLLDGKAIDAAVLALAVKGGKVRTIVSQGCKPVGEPFTITQAEDN
                     LVYALGSRPAYQVLSETFNGLSEEEKERARGNLFAGLAASEYVADLKRGDFLVRNLLG
                     ADPQTGAVAIGTRARTGQTLQYQLRDKITAHADLVELAKMKGRRGGEPFASLVFSCNG
                     RGHRFFGVPDHDAATLAEIFGPMPSAGLFCNGEIGPIGHASYLHGYTASVALFCK"
     gene            complement(4568..5458)
                     /gene="xerD"
                     /locus_tag="VNW28_04170"
     CDS             complement(4568..5458)
                     /gene="xerD"
                     /locus_tag="VNW28_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HXA09151.1"
                     /translation="MEQEIEAFIRFLAVERGLSENYQLSTQRSLGEFAAWCAQRKQIR
                     RAREVGLPLISEYLAERKRGGLSASSIKLIVVALKIFFRFATAAGDVRRDPTDALTLP
                     RIERYLPETLNELQVEQLLESVNSSAPLGLRDRAMLELLYASGLRISELAGTKLENLN
                     LDEGVVRVTGKGNKTRLVPVGRKACAALAAYLAEERPRLVKRRTGSEVFLSNRGSRLT
                     TVRIWQIVKAVARRSGLETNIYPHLLRHSFATHLLGNGADLRIIQEMLGHADISTTQV
                     YTHVDQQRLKAVHRKFHPRA"
     gene            5543..6304
                     /locus_tag="VNW28_04175"
     CDS             5543..6304
                     /locus_tag="VNW28_04175"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HXA09152.1"
                     /translation="MSLDLNTLLKDWPHEAGAIKVRKVPGLDGREKLQLRIDLGLLQM
                     EMEGRPDGQRPHNCESYLVYHQRRAVRAAERGEEYELSPEQCGELQQEGIQYYHRYLS
                     LFQIDEFEGVIRDTQRNLDLFTFVDEHSEREDLVWSFQQFRPYVLMMNTRARVSILLA
                     EGQFADAIREIESGRDRISEFFQHSAFPELEAKSSEIAFLDEWLEEVRARRPLSKLEV
                     MEREMERAIAGERYERAAELRDAIRQIKTRPEVER"
     gene            6395..7036
                     /locus_tag="VNW28_04180"
     CDS             6395..7036
                     /locus_tag="VNW28_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HXA09153.1"
                     /translation="MAASDSTLTERKERWARKMSGKAKPAVRSDSRLPPGQHLTPGFP
                     VLDLGVRPEISLGEWRLEIGGLVEKPQTFTWEQFNALPQFEDVSDFHCVTTWSKFDCR
                     WTGVAFFTLVDLVRPRAEAKHVLFTSYDGYSTNVRLSDCLDDDVLIATQFDGKPITRE
                     HGGPARIIIPKLYAWKGAKFVRTIEFSEQDKPGFWEVRGYSNTADPWTEDRFS"
     gene            7033..8007
                     /locus_tag="VNW28_04185"
     CDS             7033..8007
                     /locus_tag="VNW28_04185"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXA09154.1"
                     /translation="MSEAVPAPRKIAVLAAFAALYLIWGSTYLGIRFAINSIPPLLMA
                     GTRFFFAGIILYAIARWQGAPRSSFAEWRTALVIGACLLFFGNGSVTVAEQWVPTGLA
                     SLLVATVPIYIALLAWFTGAAPRPRPLVLLGLAGGFAGVGILVGPALFTAPGGASPHR
                     GLGMLILLFGSLLWSIGSLYSRRARNSPSPFLSSAQTMLCGGALMTLAGFLRGEARTF
                     DFRQVTALSLGAFAYLVLIGAVVGYTAYIWLLRHCDPAKVATYAYVNPLVAVLLGAFF
                     AGEILTARTALAAALIIGSVAVVITGQQANQKIPLLSRPQLHKQGETG"
     gene            8022..9188
                     /locus_tag="VNW28_04190"
     CDS             8022..9188
                     /locus_tag="VNW28_04190"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXA09155.1"
                     /translation="MKPEDAKAVAALQANWRAERETALVYRDLAANEADEKRRAILLR
                     MAEAEERHAARWENKLEELGAAPPVLKNDFRRRFNRWWNRAAGADLAIRRMEAAEDRD
                     QERYQAQRAGALGGSEDEKEFLRQTALEEKAHARILNTMSAPPPSLGPKGLLDQILKR
                     ERWHGRGGGWVADAIYGVNDGLGAVFGIVSGVAGATNNQQHYILVAGLAGMMASALSM
                     GAGAYLANKSEREIYEAEISREKAEVEENPEEEIEEMALFYQLQGFSVEESQRMAERL
                     AEQPEQMVQAMAQSELGLSQHHFPNQWTSAFSAALSTAIGAFIPIVPFFFMSGFPAVI
                     AAFAISILAHFAVGAVKSLITIRSWWASGLEMTIVGVIEAVVTYSLGLAFGALG"
     gene            9160..9966
                     /locus_tag="VNW28_04195"
     CDS             9160..9966
                     /locus_tag="VNW28_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HXA09156.1"
                     /translation="MAWPLARSDEHSLGMNLPSLAVVVPAFNEAQRLTDNLLTLLGYL
                     QTYRPTAELIVVDDGSTDGTAQVAEQLFARRPDVAARVLRFAENRGKGHAVRAGLLAT
                     QAPVALFSDADLSTPITELPKIVDPIENGQDEIVFGSRALDRSLIGHRQPWRREQGGK
                     VFNAIVRLTTGLPFSDTQCGFKAFRMEAARPIIEQGEINGFGFDVELLFLAQRAGLRL
                     REVPVRWNHHEGSKVHIVRDSLRMFSEVVSLRRRVVRDAARPTSSPEMSP"
     gene            9992..11299
                     /locus_tag="VNW28_04200"
     CDS             9992..11299
                     /locus_tag="VNW28_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09157.1"
                     /translation="MRVFSTQIVRLTLLVLCVSAGRAPAQEIRRAVPVNPPVAATAPP
                     VSTPAPATPTPPRLVPIPPPNVPAVPASVNDTAHFLAGLPVSEGSPLVALTQDPAWQR
                     HAAFFNQAWTRLDAGQLAGLRTWEASFLPDATQPLPVVFYMFSGPDLLYAEQFFPNAR
                     TYVLAGTEPIGPLPDILRYAGPSLGPALQNLDKSLNSVLNFSFFITKEMKTDLQREDL
                     KGTLPIFYVFLARSGKSITDITFITLDKSGQAHISGPGEKAKGLTPGIRITFRGAPDA
                     APQTLYYFTTDLSDDGIRSQPGFIKFCEGHGAGYSLLKSASYLMFEGGFDAVQKFLLS
                     HSKVIVQDDSGIPITDFDAAKWQLRFFGAYAGPIDVFKQHFQPQLQAIYQRIHPGPLG
                     FGIGYRWNSRQSTLIVAARKGAAVSPPQAEPPSESPPPDSTAQ"
     gene            complement(11476..11922)
                     /locus_tag="VNW28_04205"
     CDS             complement(11476..11922)
                     /locus_tag="VNW28_04205"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HXA09158.1"
                     /translation="MKKANRKSSAEKHSSYLKPALRSEAFVRALGDAKLRLAGSGGLR
                     SLFEKAARDAASLPRERFKENWPYLQTMLRLVRAYERGEYKQVSNDALLWIVAALNYL
                     VDPFDLIPDSIPFLGFVDDAIVVEFVVNKTRQTLDDFMIWETIGVS"
     gene            complement(12053..13246)
                     /locus_tag="VNW28_04210"
     CDS             complement(12053..13246)
                     /locus_tag="VNW28_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09159.1"
                     /translation="MKTNTPTKILKSVWASNVDPRLRMALCLVLCTLGLGLSVNAGGK
                     KTTITTFDAPGAGTQAGLGTFPSGINLFGAITGFTRDANSARHGFVRALDGTFTIIDD
                     PAAGTCSTSCGTIGNGQGTRAYAINLFGEITGFYTDNSGRCHGYVRAPNGTFTQIDAP
                     DAGTGPFPQGTFPSEFTPMGINLTGGITGFYVDASSVQHGFVRAPGGTITEFDPTGSV
                     FTNPNAIDDLGDITGFYVDASGVGHGFLRAHQGTITTFDAPGAGTTAGQGTFGVGLTP
                     FGEIEGVYVDASGVLHGFVRSNPGAFSTFDVPSAGTGAGQGTLPESNTPLGAITGQYI
                     DGNFVNHGFLRDTQGGFSLFDVQGTGTGAGQGTIPLDNNDLRAITGEYIDGGNVIHGF
                     LVEGL"
     gene            13448..>14793
                     /locus_tag="VNW28_04215"
     CDS             13448..>14793
                     /locus_tag="VNW28_04215"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXA09160.1"
                     /translation="MTGAIYGRTVSSVPKQRRRRLVMSFEMVRLDRVSSEPLYQQLYR
                     QIREELESGSFDSSASRVPSSRVLGATLGISRPTVNQAFSKLLEEGYLQARKRSGIFV
                     ADHLPATFLKAARPATTARTEHSPRVARRVTRMTDFRTGRQLDVGVAGPPSVTFVAGL
                     PAVDEFPIAVWERLRDQVLAKKGAHLLRYASSRGEIELRKAIAAYLCDFRGANCHPDQ
                     IVVVGGMQQAMLACALALINEGEVAWIEDPGFHQARNVFAFVGAKMVPRPIDREGMVI
                     GKCSRQNSPGLILVTPSHQFPFGVTMSLKRRKALIDFAESRDGYILEDDYNSEFRFDG
                     PPLPCLQGLDHAGRVIYAGTMSKILYPSLRLGFLVAPPQLVDTLVKVRAVMDQHSPAI
                     DQATLARFITEGFFLSHVKRIRELYAQRRVFFIEQFQKWLGDYFDLEVTPAGLHFIA"
BASE COUNT         2818 a         4712 c         4313 g         2950 t
ORIGIN      
        1 aacagagagc agcaggaaca aagccccgcc gcgcgctcag tcgatcgccc ggcgccgcga
       61 ggaatcgctc gtccggcccc gccttccgct ttcccgcctc acgaaagatt aacgtccttg
      121 ccgggatcca tctcgtagtc caacccgagg tattccgacg ttttcggctt gatcacttcc
      181 tcgacgaatt tgatgtagat gggatcttcg cgaaagatcg cctgcttgtc catcgattcg
      241 aaatcgatcg cgataaaaaa aggccacggg atcttcggat cgacccgctt gccacactta
      301 atgctcagga cctcgtcgat cttcaggagc tgcatgcgcg cgttcatcat catctcctcg
      361 gtctcttccg gcgtcacgcc cggtttcaat ttatagagtc caatgtgatg aaccatgagc
      421 ggatagctat taggcgttgc ctgagggtgg tgcaagcacg aatcggcgac cccccgcaaa
      481 cccatgtgaa catgtcaatg attcgcgccg aaggggcgat gcgacgctat tttttccagt
      541 cgaatgcgcc ttttttcagc gcataaacgt aaccgaccat caggatcgaa acgaagctca
      601 gcatgctcca gaaaatgccg tggctatggg cgaccgcttc gcgataaacc accgcccacg
      661 gatacatgaa gacgatctcc aggtcgaaga gaatgaagag catcgccacg agatagaact
      721 tcacgctgaa gcgcggctgg gcttccccct gcggcaccat cccgcactcg taagcggagt
      781 ccttcgtctg cgtccgaagg cccgatttcc caagcaacac gctgatgatc aaggcgccgg
      841 ccgcgaagcc gacggcgacc agagcttgca gcagaatcgg aatgtattcg cggatcacgt
      901 tctacgaccg cacgtgcggt tcaggccaca gccgctcgag accgcgctac cgggaagtcg
      961 cagccgctgt cgggatgtgc ccattcggcg ggagcagggc cttggcgaga gcggaaggca
     1021 aacccttgta ctttttgccg gttttcctga ccatgccacg cgcgaccagg ttctgcagtt
     1081 tctcgtaagc gcgcaggcgg agcatttcct cgccaccgct ggcggcattc cgcgcccgca
     1141 ggttctcatg caggatctcg aaaagttgtt taaactcgaa ggaagagcgg cgggaaagga
     1201 ccgcgaccag ctcttcggta acgaggtccg gcactcggcg tgaaaaagcg ttctttttta
     1261 ggatggccat aggccgcaaa tgataacata tctcggccaa accgcagcag ttttctcact
     1321 cccctgggca aatattgcgc tcgccgcctg cgccttggag gatgcgccgg gacgtcgcac
     1381 tcgtgactcg acctaaaacg ggctcgccgt cggagtcgga gtggccgccg atggcaaggg
     1441 atctcccggg ttcggctgcg acgaggcgcc gcgtttcagg ccctcctgca agtaaatgaa
     1501 cgcaccgccg atcagaccgg ccaatccaat cgtcgcctgg cgaaacgtca ctggcccgac
     1561 cgattcggtg acataaccat cggtttcgta gcgctctgac attacaatcg gcgaaagatg
     1621 tttcgtgatc gcctccgctg gcggaaattt cgccggatcg atattcttgc cgagagcggg
     1681 ataaaacgtc gcactcatca agagcagcgg acgcaccgcg gcgtccgcgc gttcgaacaa
     1741 caaacgggtg tcaacgtagt tgaatcccga gtccggttcc ggaacttggt tggcagcgtc
     1801 ccggaagagc gcgctcttct ccagttcgcc ggctggccgg gcgaccgtgg caaccgcggc
     1861 ctccaccgcc gccgcgtcag acccggcaca catcatttta tccgaaaccg cgatcgcggg
     1921 actgagcgga attaaaccgc cgagaggctg taggctgtaa acgatggcgc catctttttc
     1981 acgccgcgtc caggctgctc ccgcgatttc aacagaggtc agcgcctccg cgatcttccg
     2041 aacgcgtgcc gcgtccttta ccgggagcgt tgcctgaagc gttggccagc gcgcatcagt
     2101 cggccaatcg ccgacgattt ccagctcttc cccgaacgcc tcgcggagat cattcaatgc
     2161 catgcctcgc gccgtcattg cgttcattag ttgttgcaga atcgccggca tacgacccgc
     2221 ggccggagtc gatggtgaag aggcgttttc gggccaatgg atttgcgacg ccgagtagag
     2281 aaacgtgttc gcgccggcgg cggcgagcga gcgccgagcc agtttctctt ccgcgcccac
     2341 ccgcggcatc cccacgaaat cggtctcgcg cattttccca tgatcgaagc caagggcggc
     2401 cgccatactg cgcacctgct tcagttgcgc cagttggttc gtgaccagcg actgccccgt
     2461 cagcgcgatg actggcgtca gcttttccac aaacggtcgc ggatcaagaa agatcatccc
     2521 gccgtactcg accggcaggt gtttcagcgc cgcgctgaac gaatcgttct cccctaacga
     2581 ggcctccttt tccccccggc ggtggtcgac ccggtcgagc agcgcctcaa gcagcgcggg
     2641 atcgttcgac ccgaaaaacc agtggttgtc gtaaacgctc gcaaaaacga aatgcgaaac
     2701 gctcaccgtc tcgatcttgt gctgctggta aacgatcgtt tcgcgtttcg tctcgcccgt
     2761 tttcgggaac cactccgcct gtcgttgttc gatgaatttc cgcgcctctt ccggcgccgg
     2821 ttcgaaatga aaaccgccga ttaacttcgg ttcgttactc tcgagcgagg ccagcgcgat
     2881 gaaaccgtgc gttggcccga gctggagaaa ttcctccagt gtcttgcgac cgccgccatc
     2941 ctgcggcagc cgtgcgagcg gttcctgcaa acacgcctgc accgacggtt cccgccagat
     3001 ctggtaaatg tcgctctcat gccattgctt gcgcgtttgc tgaaaatcgg gtacgagggc
     3061 cagcgccagg gtcgtttttg gcaggagctc cggcacggtc gcgaggcgct gcgcgagctg
     3121 cacccgcaag atcacccaga cggctcccgc gaccaccagg gcgatcacga tcacggcggc
     3181 taatttcttc atcggcgcag tctcgcacca aattgcgccg tcgcgagccg tcgtccgcgc
     3241 tcgacttttt gccgtgcatc gacgatctcc atggatggaa gcgtgcgccg gttcgcgact
     3301 cattctgggc gagtttcgtg aaagcgcggt cgtcgccgcg gcggagcagg ccctcgccga
     3361 atgcggcggt caaccgacct gcggcttcct ttttctctcc cccagctggc tgccgcagat
     3421 ccctgaagtg ctcgatctcg tccggctgca cggacggatt ccgctcctcc tcggatcggt
     3481 cggggcgggt ctgattggcc ggagccagga agccgagggc gtcgccggaa tgtcgttgct
     3541 tctcgttagg ctgccggaga ccaaactgac gccggttttg atttcgagcg agcaaatcga
     3601 agaatcgacc gggcccgcct actggcagat ggagaccggc atcggcccgg atgacgtcga
     3661 ttcctggatc gttctcgcca acccctatgc gcccgacttg gagcgctggc tggcggaatg
     3721 gaatgtcgct tttcccgatg cgcccagcat cggcggcctt tccagtggcg ccggtgacgg
     3781 cggggaaaag gcgcttctcc tcgatggcaa agcgatcgac gcggcggtcc tcgcgctcgc
     3841 ggtgaagggc ggaaaagttc gcaccatcgt gagccagggc tgcaaaccgg tcggcgagcc
     3901 cttcaccatt acccaggcgg aagacaacct cgtctacgcc ctcggttccc gccccgctta
     3961 ccaggtcttg agcgaaacct tcaacggctt gtccgaagag gagaaggagc gcgcccgcgg
     4021 caatctcttc gccggcctgg ccgcttcgga atacgtcgcc gacctgaaac gcggcgattt
     4081 ccttgtccgc aacttgttag gtgccgatcc gcaaaccggc gcggtcgcga tcggcacccg
     4141 cgcccggacc gggcaaactt tgcagtacca attgcgcgac aaaatcaccg cccacgcgga
     4201 cctcgtcgaa cttgccaaga tgaaaggccg gaggggcggg gaaccctttg cctcgctcgt
     4261 cttttcctgc aacggccgcg gccatcgctt ctttggcgtg cccgatcacg atgccgcgac
     4321 gctcgccgag attttcgggc cgatgccgag cgcgggactt ttctgcaacg gcgaaatcgg
     4381 cccgattggc catgccagtt acctccatgg ttacaccgcg tcggtcgcgc ttttctgcaa
     4441 atagggcagc tcgcccagcg cacgccgccc gtaactcagg cttccggcct gacacgacga
     4501 gcaggcttcc agcctgctga ctgtgcgggc aggattcccg ctcgacgaaa caggcaggat
     4561 gcctgtgtta cgcgcggggg tggaacttgc gatgcaccgc cttcaggcgc tgctggtcga
     4621 cgtgggtgta gacctgggtc gtcgagatat cggcgtgccc gagcatttcc tggatgatgc
     4681 gcagatcggc gccgttgcct aacaagtggg tcgcgaaact gtgccggagc aagtgcggat
     4741 aaatattcgt ctccagcccc gagcgccggg cgaccgcctt gacgatctgc cagatccgca
     4801 cggtcgttag gcgggagccc cggttggaaa gaaaaacttc gctcccggtc cgtcgcttga
     4861 cgagacgcgg ccgttcctcc gcgagatagg cggccagcgc cgcgcaggcc ttgcgcccaa
     4921 ccggcacgag tcgcgttttg tttcccttcc cggtcacccg caccacgcct tcgtcgagat
     4981 tcaggttctc cagtttcgtc ccggccagtt cggaaatgcg cagcccgctg gcgtaaagca
     5041 actcgagcat cgcgcggtcg cgcaagccga gcggtgcgct cgaattcacg ctttcgagca
     5101 actgctcgac ctgcagttcg ttcagcgtct ccggcagata acgctcaatc cgcgggagcg
     5161 tcagggcgtc ggtcggatcg cgtcgcacat cgcccgcggc ggtcgcaaag cgaaagaaaa
     5221 tcttgagcgc gaccacgata agtttgatcg acgaggcgga aagcccgccg cgcttccgtt
     5281 ccgccagata ttcgctgatc aagggcaacc cgacctcgcg cgcgcgccgg atctgtttcc
     5341 gctgcgcaca ccaggcggca aattcgccta acgaacgctg ggtggagagc tggtagtttt
     5401 cggacagacc gcgctccact gccaggaagc gaatgaaggc ctcgatctcc tgctccatcg
     5461 tcggcattgt ccgcaagggt cgctcccttt gcaatccggc gcgcgttgac aaagcaaacc
     5521 cggactcgat tgtttcctcg cgatgagcct tgatttgaac acgctcctga aggattggcc
     5581 gcacgaagcc ggcgcgatca aagtccggaa agttcccggt ctcgacggcc gcgagaaact
     5641 ccagctccgg atcgatctcg gcctcctgca aatggaaatg gaaggccggc ccgacggtca
     5701 gcgcccgcac aactgcgaat cgtacctggt ctatcaccag cgccgggcgg tgcgcgccgc
     5761 ggaacggggc gaggaatacg aactctcgcc cgagcaatgc ggcgaattgc agcaggaagg
     5821 catccagtat taccaccgct atctgagcct gtttcagatc gatgaattcg aaggcgtgat
     5881 ccgcgatacg caacgcaacc tcgatctctt tactttcgtc gacgaacaca gcgagcggga
     5941 ggatctggtc tggagctttc aacagtttcg gccctatgtc ctgatgatga acacccgcgc
     6001 ccgggtttcg attttgctcg ccgagggaca atttgccgat gccattcggg agatcgaaag
     6061 cgggcgcgac cggatcagcg agtttttcca gcattcggct tttccggagc tggaagcgaa
     6121 gagttccgaa atcgcatttc tcgatgaatg gctcgaggaa gtgcgcgcca ggcggccgct
     6181 ctcgaaactg gaagtgatgg agcgcgagat ggaacgcgcc atcgccggcg aacgctacga
     6241 acgcgccgcg gaattgcgcg acgcgatccg ccagatcaag acccggcccg aggtcgagcg
     6301 ttgagtccgc ctcggaggga cgtgcttccg cgcgtccggt ttctttcgtc ggaaaaggca
     6361 atatccagga cgtgccgaag cacgtcgctc cgcgatggcg gcatctgatt cgaccctcac
     6421 cgagcgcaag gaacgctggg cccgtaaaat gagcgggaaa gcgaaacccg ctgtgcgctc
     6481 ggacagtcgg ctcccgcccg gacaacatct tacccccggc ttcccggtcc tcgatctcgg
     6541 ggtgcggccg gaaatatcgt taggcgaatg gcggctggaa attggcggcc tggtggaaaa
     6601 gccacaaacg ttcacctggg aacaattcaa cgctctcccc cagttcgaag acgtgagcga
     6661 ttttcactgc gtcaccacct ggagcaagtt cgattgccgc tggacaggcg tcgccttttt
     6721 caccctcgtc gatctcgtta ggccaagagc cgaagcgaaa catgttcttt tcaccagtta
     6781 cgacggctac agcacaaacg tgcgcctgtc cgattgcctc gacgacgacg tcctgatcgc
     6841 gacccagttc gacggcaaac cgatcacgcg cgagcatggc ggcccggcgc ggatcatcat
     6901 tccgaagctc tacgcctgga aaggcgccaa gttcgtgcgg acgatcgagt tttctgagca
     6961 agacaagcct ggtttctggg aagtccgtgg ctacagcaac accgccgacc cttggacgga
     7021 agatcgcttc tcgtgagcga agcggtcccc gcgccacgca aaatcgcagt cctggccgct
     7081 ttcgccgcgc tttacctcat ttggggttcc acttatctcg ggattcgctt cgcgatcaat
     7141 tccatcccgc cgctcctcat ggcgggcacg cgcttttttt tcgccgggat catcctctac
     7201 gccatcgccc gctggcaggg cgcgccgcgc tcttcctttg cggaatggcg gacggctttg
     7261 gtcatcggcg cctgcctgct ctttttcggc aacggcagcg tgacggtcgc ggagcaatgg
     7321 gtgccgaccg gtctggcttc gctcctggtc gcgacggtgc cgatttatat cgcgctcctc
     7381 gcctggttta ccggcgcggc gccccggccg cggccgctcg tcttgctggg gcttgcggga
     7441 ggctttgccg gcgtcggaat cctcgtcggt ccggccctct tcaccgctcc gggcggcgct
     7501 tcaccgcacc ggggtttggg aatgttgatt ctccttttcg gttcgcttct ctggtcgatc
     7561 ggttcgctct attcgcgccg cgcgcgcaac tccccttctc cgtttctttc ctccgcgcaa
     7621 accatgctct gcggcggcgc gctcatgacg ctcgccggct tcctgcgcgg cgaagcgcgc
     7681 acttttgatt ttcgccaggt gaccgctttg tcgttaggcg cgttcgccta tctcgtcctc
     7741 atcggcgcgg tggtcggcta taccgcttat atctggctgc tccgccattg cgatccggcc
     7801 aaggtcgcca cctacgccta cgtcaatccg ctggtggccg tcctgctcgg cgcatttttt
     7861 gccggcgaaa tcctcactgc ccggaccgcg ctcgccgcgg cgctcatcat cggatcggtc
     7921 gcggtcgtga tcacggggca gcaggcgaat cagaaaattc cgcttctctc gcgcccgcag
     7981 ttgcacaagc aaggtgaaac cggctaatag agcgtgacgg aatgaagccg gaagatgcca
     8041 aagcggtcgc cgccctccag gccaactggc gcgcggaacg ggaaacggcc ctggtttatc
     8101 gcgacctcgc cgccaacgag gccgacgaaa agcgccgtgc catcctcctg cggatggcgg
     8161 aagcggagga gcgtcacgcg gcgcgctggg aaaataaact ggaggagctc ggcgccgcgc
     8221 cgccggtctt aaaaaatgat ttccggcggc gtttcaaccg ctggtggaat cgcgccgcgg
     8281 gcgcggacct ggccattcgc cggatggaag cggcggaaga tcgcgaccag gagcgctacc
     8341 aggcgcagcg cgccggcgcg ctcggcggga gcgaggacga aaaggaattt ctgcgccaga
     8401 ccgcgctcga ggaaaaggcc cacgctcgca ttcttaatac catgagcgcg cccccgccct
     8461 cgttaggccc gaagggtttg cttgaccaga tcttgaaacg cgagcgctgg cacggacgcg
     8521 gcggcggttg ggtggcggac gcgatttacg gcgtcaacga cggtctcggc gcggtcttcg
     8581 gcatcgtttc cggcgtggct ggcgcgacca ataaccagca gcattacatc ctcgtcgccg
     8641 gcctggccgg catgatggct tcggctcttt ccatgggcgc cggcgcctat cttgcgaaca
     8701 aaagcgagcg cgaaatttac gaggccgaga tctcgcgcga gaaagcggag gtcgaagaaa
     8761 atcccgaaga ggaaattgaa gagatggcgc tcttttacca gcttcaggga ttctctgtgg
     8821 aggaatcgca gcgcatggcg gagcgcctcg ccgagcagcc cgagcagatg gtccaggcga
     8881 tggcacagag tgagctcgga ttatcccaac accattttcc caatcaatgg acctcggctt
     8941 tttccgcagc cctttcgacc gcgatcggcg cttttatccc gatcgttcca ttcttcttca
     9001 tgagcgggtt ccccgcggtg attgcggcct tcgccatcag catcctggcg cacttcgcgg
     9061 tcggcgcggt caaatcgctc atcaccatcc gctcctggtg ggcgtccggt ttggaaatga
     9121 cgatcgtcgg tgtgattgaa gcggtcgtga cttacagcct tggcctggcc tttggcgcgc
     9181 tcggatgaac actcgttagg catgaacctc ccgtcgctcg ccgtcgtcgt tcccgctttt
     9241 aatgaagcgc agcgcctgac cgacaatctg ctcaccctcc tcggctattt gcagacttat
     9301 cgcccaaccg cggaattgat cgtagtcgac gacggatcga cggacggcac cgcacaggtc
     9361 gccgagcaac tttttgcccg gcggcccgac gtggcggcgc gcgtcctgcg cttcgcggag
     9421 aatcgcggca aaggccacgc cgtccgcgcc ggcctgctcg ccacgcaggc gccggtcgcg
     9481 cttttctcgg acgccgatct ttcgaccccg atcaccgaac tgccgaagat cgtcgacccg
     9541 atcgaaaacg ggcaggacga gattgtcttt ggctcccggg cactggatcg cagcctgatc
     9601 gggcaccgtc agccgtggcg gcgcgagcag ggagggaagg tcttcaacgc gatcgtccgg
     9661 ctcacgaccg gtcttccgtt ttccgacacg caatgcggtt tcaaggcttt ccggatggaa
     9721 gccgcgcgcc cgatcatcga gcagggggag atcaacgggt tcggcttcga cgtcgagctg
     9781 ctcttcctcg cgcaacgggc cgggttgcgc ctgcgggagg tgccggtgcg ctggaatcat
     9841 catgaaggca gcaaggtgca catcgtgcgc gacagcctgc gcatgttctc cgaggtggtg
     9901 agcctgcgcc ggcgggtcgt ccgcgacgcg gcccgcccga cctcttcgcc ggagatgtcg
     9961 ccctaacgaa ccacacaaag gcccaaaaat cgtgagagta ttctcaaccc aaatcgttag
    10021 gctgacgctc ctcgtcctct gcgtgtccgc gggccgcgcg cccgcgcagg aaatccgccg
    10081 cgccgttccg gtgaatccgc cggtcgcggc gacggccccg cccgtctcga ccccggcccc
    10141 ggcgacgccc acgccgccgc ggctcgtccc catccctccg cctaacgtcc ctgcggtccc
    10201 cgcgtcggtc aacgacaccg cgcatttcct cgccggcctc ccggtctcgg agggctcccc
    10261 gcttgttgcc ctcactcagg atcccgcctg gcagcggcac gccgcttttt tcaaccaagc
    10321 ctggaccagg ctcgacgcgg gccagctcgc tggcctccgc acctgggaag ccagcttcct
    10381 gcccgatgcc acccagccgc tcccggtcgt cttctacatg tttagcgggc cggacctcct
    10441 ctatgccgaa caatttttcc cgaacgcccg cacctacgtc ctcgccggca ccgagccgat
    10501 cggcccgttg cccgatatcc tgcgctacgc cggtccctca ctcggccccg ccctgcaaaa
    10561 tctcgataaa tcccttaaca gcgtcctcaa tttttctttc ttcatcacca aggagatgaa
    10621 gaccgacctg caacgcgagg atctcaaagg caccctcccg atcttctacg tcttcctcgc
    10681 ccgttccggc aaaagcatca ccgacattac cttcatcacc ctcgacaaaa gcggccaggc
    10741 ccacatcagc ggaccaggcg agaaagccaa aggccttacg cccggcatcc gcatcacctt
    10801 tcgcggcgcg ccggatgcgg cgccccagac tctttactac ttcaccaccg acttgtccga
    10861 cgacggcatc cgatcccagc ctggtttcat aaaattctgc gaggggcacg gcgccggcta
    10921 cagcctgttg aaatcggctt cctatctcat gttcgaaggc ggctttgacg cggtgcaaaa
    10981 gtttctactc agccacagca aagtcatcgt gcaggatgat tccgggattc cgatcaccga
    11041 ctttgacgcc gcgaaatggc aactgcgttt cttcggcgct tacgccggcc cgatcgacgt
    11101 cttcaaacag catttccagc cgcaactgca ggcgatctat caacgcattc atccgggccc
    11161 gctgggtttc gggattggct accgctggaa ctcgcggcaa tcgaccctca tcgtcgccgc
    11221 gcgcaaaggc gccgcagtat cgcctccgca agccgagccg ccgtctgaat ccccaccgcc
    11281 cgattccacg gcgcaatagc ccgcgtcgcg cccgccccag acgggaagag gaagcgagtc
    11341 caaagctccc cgcgaatctt caacccaata aaccaacgaa ctaaatccca ccaaacccgc
    11401 tgccgcgctt gactctcttc ataggggtta gatgcgttca cttcgctcgc agagacggat
    11461 actccccttc gatcttcaac tgacgccaat ggtttcccag atcatgaaat cgtccagcgt
    11521 ttgccgggtc ttattcacga caaattcgac aacaatcgcg tcatcgacga agccgagaaa
    11581 cggaatgctg tccggaatca aatcgaatgg atcgaccaga tagttgagag ccgctacaat
    11641 ccacagcaga gcatcattcg aaacctgctt gtattccccg cgctcgtagg cgcggaccag
    11701 ccgcagcatc gtttggagat acggccaatt ctccttaaat cgctccctgg ggagcgaggc
    11761 ggcgtctctc gcggcctttt caaaaagcga gcgcaaaccg ccggaaccgg cgaggcgcaa
    11821 tttcgcgtcg ccgagcgccc gaacaaacgc ttcgctgcgc agggctggtt tcagatagga
    11881 actgtgcttt tcggcgctcg atttcctgtt cgcctttttc atctgacgct atgaaagcac
    11941 ctcgatgcgc caatgagaag ccaattcggt ggtggtacag ttgtaccacc accgccaatt
    12001 tgcaccagaa gcgacttact tgcgactcag gtttgtccaa ttccaggtag gcttacaagc
    12061 cttccacgag gaagccgtgt atcacattgc cgccgtcaat atactctccc gtgattgctc
    12121 tcaggtcgtt gttgtccaag ggaatggtgc cctggccggc gcctgtgccc gtaccctgga
    12181 catcgaacaa gctgaagccc ccttgcgtat cgcgcaggaa gccgtgattc acgaagtttc
    12241 cgtcgatgta ctgtcccgtg atcgccccca ggggggtgtt gctttcaggg agggtgccct
    12301 ggcctgcccc tgtgcccgca ctcggaacat cgaacgtgct gaaggcgccc ggattagacc
    12361 ggacgaagcc gtgaagcaca ccgcttgcgt cgacgtagac tccttcgatc tctccgaacg
    12421 gagtgaggcc tacgccgaat gtgccctggc ctgccgttgt gcccgcgccc ggggcatcga
    12481 acgtcgtgat cgtaccctgg tgagcgcgca gaaagccgtg acccacaccg ctggcgtcaa
    12541 cgtagaatcc cgtgatgtca cccaggtcat cgatagcatt gggattggtg aatacggagc
    12601 ccgtgggatc gaactcggtg atcgtgccgc caggagcgcg cacgaagccg tgctgtactg
    12661 agcttgcgtc cacgtagaat ccagtgatcc cgcctgtcag gttgatgccc attggagtga
    12721 attcagaggg gaaagtcccc tggggaaacg gtcctgtgcc cgcgtccgga gcatcgatct
    12781 gggtgaacgt gccgttagga gcgcgaacgt agccgtgaca tcgtccgcta ttgtcggtgt
    12841 aaaatcccgt gatctcccca aataggttga tggcgtaagc acgcgtgccc tggccgttcc
    12901 cgatggttcc acaggatgtt gaacaagtgc ccgcggccgg gtcgtcgatg atggtaaagg
    12961 tcccgtcaag agcgcgcaca aagccgtgcc gggcagagtt ggcgtcccgg gtgaatcccg
    13021 ttatcgcccc gaaaaggtta atgccactag ggaaggtgcc cagacctgcc tgtgtgcccg
    13081 cgcccggcgc atcgaacgtg gtgatggtcg tcttctttcc gccagcattc acggaaaggc
    13141 ccagccccaa cgtgcagagg accaggcaaa gcgccattcg tagacgcggg tcaacgttcg
    13201 acgcccaaac agatttaagg attttggtag gagtgttggt tttcatagga tttgttgtga
    13261 tggacattgt tttgactgaa cattgcgttc attggaaaga ggctggcaat aaccagatcg
    13321 cgctaaaaag gctgaccagt ttcgcttgtc gcgggaacca agggctttga gctacgcagc
    13381 accggagaat tgtgcagctt ctcatcttga gaaagcattc tctggatgcg aaagtcgcgt
    13441 cgtgaaaatg actggcgcga tctacgggcg cacggtctca tcggtcccaa agcagcgtcg
    13501 gcgccgttta gtcatgtcgt tcgaaatggt gcggctggat cgcgtgtcgt ccgagccgct
    13561 ttaccaacaa ctgtatcgtc aaatacgaga agaacttgag agcggcagtt ttgattcaag
    13621 cgcttcccgt gtgccttcct cacgagttct cggcgcaact ctcgggattt cgagaccgac
    13681 cgtaaaccag gcgttctcaa agctgcttga agagggctat cttcaagccc ggaaacggtc
    13741 tggtatcttt gtcgccgatc atttacctgc aacttttctg aaggctgcca ggccagcgac
    13801 gaccgcgcgc accgagcatt ccccgcgtgt cgcccgacga gtgaccagaa tgactgattt
    13861 tcgcacgggc cgacaactcg atgtcggcgt tgccggcccg ccgagcgtca cattcgtcgc
    13921 ggggttgccg gccgtggatg aattcccgat tgccgtttgg gaacgattgc gcgaccaggt
    13981 tttggcaaaa aagggagcgc acctattgcg ttatgcgtcg agccgtggcg agatcgaact
    14041 tcggaaggca atcgctgctt acttgtgcga cttccggggc gccaattgtc atcccgacca
    14101 aatcgtagtc gtgggcggca tgcagcaggc gatgctggcg tgcgcgctcg cgttaatcaa
    14161 tgagggtgaa gtggcatgga tcgaagatcc cggcttccat caggcacgaa atgtcttcgc
    14221 ttttgtcggg gccaaaatgg tcccgcgccc aatcgatcgc gaggggatgg taatcgggaa
    14281 gtgctcacgg caaaattctc ccggactcat tctcgtcacg ccgtcgcacc agtttccctt
    14341 cggtgtgacg atgagcctta agcgacggaa agctctaatc gacttcgcgg agtcgcgcga
    14401 cggctacatc ctggaggatg attataattc cgagtttcgt ttcgatgggc cgccactgcc
    14461 atgccttcaa ggtttggacc atgccggccg ggtgatttat gccggcacga tgagcaagat
    14521 cctttaccca tcgctgcgcc tcggttttct ggtcgcaccg ccacaacttg tcgacaccct
    14581 cgtcaaggtg cgcgctgtca tggaccagca ctccccagcg attgatcaag ctaccctggc
    14641 gcgtttcatc accgagggat ttttcctcag ccatgtgaaa cggatccgcg aactctatgc
    14701 ccagcgacgc gtattcttta tcgaacaatt ccaaaaatgg ctgggcgact atttcgatct
    14761 ggaagtaacg ccagccggtt tgcacttcat cgc
//
LOCUS       DATMNS010000114        10882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1595115, whole genome shotgun sequence.
ACCESSION   DATMNS010000114 DATMNS010000000
VERSION     DATMNS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 10882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10882
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1595115"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /gene="rpsT"
                     /locus_tag="VNW28_04220"
     CDS             <1..215
                     /gene="rpsT"
                     /locus_tag="VNW28_04220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXA09161.1"
                     /translation="RRTLRNRSVITNLRSLTKRQATGGGKPEENRALVSALDKAAKRG
                     IIHKNVANRRKARLNKAAAKGSASPS"
     gene            331..921
                     /locus_tag="VNW28_04225"
     CDS             331..921
                     /locus_tag="VNW28_04225"
                     /inference="COORDINATES: protein motif:HMM:NF042461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6580 family putative transport protein"
                     /protein_id="HXA09162.1"
                     /translation="MISALFLVFLAVLYRIATAIIVQGGGSAWLSNFAPLAAIALCGA
                     IYFPRKYKFTVPFAALLVSDVVLDFYYGASLFDPLIFCRYFAFALVGLLGLAISRRAS
                     LKTIIPASLVGSALFYAITNAFSWLTDPGYVKNFSGLIQALTVGLPQYGATPTWMFFR
                     NSLVSDLLFTLLFVACMSFSRRPAARPAGAARPLPN"
     gene            927..1946
                     /gene="pdhA"
                     /locus_tag="VNW28_04230"
     CDS             927..1946
                     /gene="pdhA"
                     /locus_tag="VNW28_04230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HXA09163.1"
                     /translation="MPAPEQRDEVEMLATMLLIRRFEERASQQYQAQKIGGFCHLYIG
                     QEAVVTGAVAATRPDDYLITAYRDHAHALARGTSADACMAELFGKATGCSRGLGGSMH
                     YFDKANHMYGGHAIVGAHVPLAAGMAFAAKYRKEDRVTLCFFGDGAINQGGFHEALNL
                     AGLFKLPVIFICENNFFAMGTSVKRSTSLQHIVDRAEGYDMQAEIVDGMNFREVRDKL
                     GEIIASIRKEPHPAFVEARTYRYRGHSMSDPASYRTKEELEKYRLDDPITRLRAQLTR
                     EGKLTNEQFDKMDKKAKEQALASVKFAGESAEPPLDKLYDYTYADAVEAGVSPARKAS
                     SGRKR"
     gene            1943..2920
                     /locus_tag="VNW28_04235"
     CDS             1943..2920
                     /locus_tag="VNW28_04235"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HXA09164.1"
                     /translation="MREITYRQALNEALAEEIARDPNVFLMGEEVAEYNGAYKVSQGL
                     LDRFGAERIIDTPISENGFAGLGIGAAMVGLRPIVEFMTFSFSLVAFDQIVNNAPNML
                     TMSGGQFNIPITFRGPNGPAHQLGATHSHATECFYANVPGLKICTPATPRDAKGLLKT
                     AVRDNNPVLVLECELFYSHKGMVEDDGVDLLVPLGEAEIKRAGSDVTIICYAQTVPMA
                     LAAAETLAGEKINAEVLDLRSIKPLDLPAILQSVAKTHRVVIAEQDRPFCGVGAEITY
                     RIQKEIFDELDAPIMRVAQEDVPMPYNERLEQAVLPNAAKIVAAVEKIC"
     gene            2954..4189
                     /locus_tag="VNW28_04240"
     CDS             2954..4189
                     /locus_tag="VNW28_04240"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXA09165.1"
                     /translation="MPEIQMPKLSDTMTEGTLVSWKKKIGEQVTAGEVLAEIETDKAT
                     MEWEATDDGVLQEIFVPEGGKANVGERIAFIGDGNETAPEAEKPAPAPGKGPDKKPAA
                     ETKAKPVEAGVPPAPEQPVGRIKASPLARKIAEEKNVDLTIIKGTGPGARILEKDVEA
                     MVLAAGRPSKTKTAAPPISPGESARISLTGMRRIIAERLVESKGPVPHFYLNIEIDAD
                     ALMRVRAELKSAGDDDDATKITVNDFILKGAVVAALREPKVNAAFDGDAVIHYGDINL
                     AVAVAIDEGLVTPVIRAAQDKSLREISAVVKDFAHRARNKRMKPEEFQGGTLTVSNLG
                     SYGIDDFLAIINPPQAVILAIGAIVRKPVVNEAGAIVPGYRMSIGMSCDHRVVDGAIG
                     AEYLKALRHVLENPSLLLL"
     gene            4252..4614
                     /locus_tag="VNW28_04245"
     CDS             4252..4614
                     /locus_tag="VNW28_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09166.1"
                     /translation="MSSPRDTPPPNEKKLRVVSFAIHATRGLLRDQSARRWTMFGLVC
                     AALVLLSCGATFLGPVLDPRSRPGWFIFYWFVCAWITASAALLAVFDLLLVRARARAV
                     KRALEEQISAPPKASNAD"
     gene            4604..5755
                     /locus_tag="VNW28_04250"
     CDS             4604..5755
                     /locus_tag="VNW28_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HXA09167.1"
                     /translation="MQTNEFARGAALPPTGWKRREQMEADERRTLAPFAQKSGDSRGR
                     HFPEERHLYRTEFQRDRARVIHSRAFRRLEYKTQVFLNGTADHLRTRLTHTFEVASVS
                     RTLARALALNEDLTEAIALAHDLGHAPFGHSGEEMLAECMREHGGFEHNRQSLRVVEL
                     LETAYPAFPGLNLTYEVLEGLRKHDEGAAPSLEAQIANLADEITYYSHDLDDALDFAI
                     LDSDQLDESAIWQRSHERVATRSPGLLGTELHKNIIRDIIDLQVRDVVATSATAIAAA
                     GPTSVEEVRGRKEPLIRYSDEQGVANRDLRRFLYENVYYHPRVAEVNRRACEMLRAVF
                     VAFVREPERLGDAATRRIEGEGLHRTVCDYIAGMTDRYLLEEHARLARG"
     gene            complement(5771..6397)
                     /locus_tag="VNW28_04255"
     CDS             complement(5771..6397)
                     /locus_tag="VNW28_04255"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXA09168.1"
                     /translation="MSETVELPATVPVMPLTGVLLFPNALLPLHIFEPRFRDMLEHAL
                     QDDRMLCVALVRPEREQWRTSADFFPVSTVGLIRACVGRSDGTSDLILQGIRRVKFSE
                     FEQEVPFPIARIEALKTRTKPSVETDALAAKVLEFYAHLKESGRELPEKIDRYLSELS
                     DPEMLADLIASTFINDSARRQQLLEELDLNQRLRLLIQFLREENGGVP"
     gene            complement(6417..7112)
                     /locus_tag="VNW28_04260"
     CDS             complement(6417..7112)
                     /locus_tag="VNW28_04260"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HXA09169.1"
                     /translation="MFNKRSVTNRASVSRVVGVIASPAALARAARLRHPPDLFELRLD
                     ALYQSLGEVARVLPKLRAPLILTARHPAEGGCGNLNLAARRALLLRFLGSAAFVDLEF
                     RSLRQMKALIAQMRRRRIGLIISRHDLRDTPPPNELRRQAKLAAAARPAIFKVATRTD
                     TPAQLARLISFFETTRTPLPMAAMGIGKLGLASRRQLARLGSVLIYVSLGEANISGQP
                     SLRQLRRDRRAYN"
     gene            7174..8565
                     /locus_tag="VNW28_04265"
     CDS             7174..8565
                     /locus_tag="VNW28_04265"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HXA09170.1"
                     /translation="MLARQQELSTQFQQLLDAFLLAGALFAAHALRFYGTTWLDLPYS
                     IDPFRNYQWLLVVIMPFGPIILDLQGFYLAPLNKTLWQSFVQVARTMIYLSILVSGCV
                     IFLRLPLTSRAVPLLFILLATAMLLMKQRLIVRRIRRRAERGEVRERVLLAGVPQDIA
                     ALEQSLTPEETMVLQVAERIDIEKQPISDLVEAMHRHTVSRVIFAAGHGQLNRVEQAI
                     GACEVEGVPAWLVANFIQTAIAKPDFDAFGGRPMLVFRSTPDVSWAFRLKGLIDRVGA
                     LLGLIVLAVPMLIVALGIRLTSKGPIIFKQERAGKNGKPFVMYKFRSMSSDAEMRRAE
                     LLPFNQMRGPVFKVADDPRITSLGRWLRRTSMDEVPQLLNVLKGNMSLVGPRPLPLYE
                     VEKFENTAQRRRLSVKPGLTCLWQISGRNQVMDFSDWVKLDLEYIDRWSLGLDFKILC
                     RTIPAVLFGLGAK"
     gene            complement(8665..9261)
                     /locus_tag="VNW28_04270"
     CDS             complement(8665..9261)
                     /locus_tag="VNW28_04270"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HXA09171.1"
                     /translation="MFTGLIEEVGSVRWLRATERGTQLQLAAPLIAPGLRVGDSVSVN
                     GCCLTATAIRAGNLTFDLLEETLARTNLRTLRPASPVNLERALAADGRLGGHFVQGHS
                     DCAARALSFAPHGVDYRLEVELPGEFAHYAAFKGSIALNGVSLTIAELLPASFAVWII
                     PHTRRQTNFETLQAGDLVNLEFDLLAKYVERMLPRRIL"
     gene            9311..9562
                     /locus_tag="VNW28_04275"
     CDS             9311..9562
                     /locus_tag="VNW28_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09172.1"
                     /translation="MPTCRSVQDQPNSVCLPEANRIDPDWKEGTAHLWCRAGTLSGAT
                     VAFLRTTQVVINSLGAGLTAGFLAKKILLAGAKIAQIAR"
     gene            9730..>10882
                     /locus_tag="VNW28_04280"
     CDS             9730..>10882
                     /locus_tag="VNW28_04280"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXA09173.1"
                     /translation="MEKPMIKNLTKTIGVLRAALVAGVGFPFLMGASLYAQAPATTAP
                     AGGEATTERITVTGSLIPTAEEVTASPLDTLNTSDIAVSGGTADVLTILQKRNPDFVG
                     GGNLGGSNANIASGATQGGSIISLRGLPTLVLLEGRRIADSAAISAGGFQFTDVSLFP
                     TSLISRIEVLKDGASAEYGSEAVGGVVNIFLKKDFEGVEIGARYGFSVSSAVAERKAY
                     AIAGVGNDTTHVTAGFQYYEIDPLFNRERGYSRPFVGGTTTFPGGGRDEAGGATNFYI
                     LKSNLNSPFDAPGVTPGGVIVGSNTGAYSQIPQAYNNVPFTTANPGFIPGGFLGFDLS
                     KLPTSTIDISNTNAYASFTHQIFGKQLEIFGDFLYAHNHNESFLNGQPLN"
BASE COUNT         2138 a         3180 c         3430 g         2134 t
ORIGIN      
        1 ctcgtcgcac tctgcgcaat cgtagcgtta ttaccaatct tcgttcgctc accaaacggc
       61 aggcgacagg tggtgggaag ccggaggaga accgcgcgct cgtctccgcg ctcgataaag
      121 cggcgaaacg cgggattatt cacaaaaacg tggccaatcg ccgcaaggcc cggctgaata
      181 aagccgccgc gaagggttcg gcctccccct cgtaatcgta ctcttgctcg tgatcgactt
      241 caccggcgat cgcttacgat cgcgagcgtg agcaggggca cgggaaaaga aacccgattg
      301 catctcgtcc agaacagttg agtttcgccg atgatttctg cgctttttct tgtctttctc
      361 gcggtccttt atcgcatcgc cacagccatc atcgtgcagg gcggcgggag cgcatggctt
      421 tcgaatttcg cgccactcgc agcgatcgct ctttgtggcg cgatctattt cccgaggaaa
      481 tacaagttca ccgtcccctt cgcggcgctg ctcgtttcgg acgtcgtcct cgatttttat
      541 tatggggctt ccctctttga tcctctgatc ttctgccgct actttgcctt cgcgctggtc
      601 gggttgctcg ggctggccat cagccggcgc gcttcgctca agaccatcat cccggcctcg
      661 ctcgtcgggt ccgctctttt ttacgcgatc acgaacgcgt tttcctggtt gaccgatccc
      721 ggctatgtaa aaaatttctc cgggttgatc caggcgctga ccgtcggtct cccacagtat
      781 ggcgcgacgc cgacatggat gtttttccgc aactcgctcg tgagcgattt gttgttcacg
      841 ctcctctttg tcgcttgcat gagcttcagc cggcgccctg ccgcgcggcc cgcgggcgcc
      901 gcccgtcccc tgcctaacta attcaaatgc cggcgccgga gcaacgcgac gaagtcgaga
      961 tgctcgcgac gatgctgctg atccgccgct tcgaagagcg cgcgagtcag caatatcagg
     1021 cgcagaaaat cggcggcttc tgccatctct acattggcca ggaagcggtc gtgaccggcg
     1081 cggtcgcggc cacccggccc gacgattacc tcatcaccgc ctatcgcgat catgcccatg
     1141 ccctggcccg cgggacgagc gcggacgcct gcatggcgga gctctttggc aaggcgaccg
     1201 gctgttcgcg cgggctcggc ggctcgatgc actattttga caaggccaat cacatgtatg
     1261 gcggtcacgc catcgtcggt gcgcatgtcc cgctcgcggc cggaatggcg ttcgcggcga
     1321 aataccgcaa ggaagaccgg gtcacgctct gcttttttgg cgacggcgcg atcaaccagg
     1381 gcggttttca cgaggcgctc aatctcgcgg gccttttcaa gctgccggtc atcttcattt
     1441 gcgaaaacaa cttctttgcc atggggacat cggtgaaacg ctcgacctcg ctccagcaca
     1501 tcgtcgatcg cgccgagggc tacgacatgc aggccgagat tgtggacggg atgaactttc
     1561 gcgaggtgcg cgacaagctg ggggaaatca tcgcatcgat ccggaaagaa ccgcacccgg
     1621 cctttgtcga ggcgcgcacg tatcgttatc gcggccattc gatgtctgat ccggccagtt
     1681 atcggacgaa ggaagaactg gagaaatacc ggctcgacga tccgatcacg cgtctgcgcg
     1741 cgcagctcac ccgtgaagga aaactgacga acgagcagtt cgacaagatg gacaaaaagg
     1801 cgaaggagca ggcgctggcg agcgtgaagt tcgccgggga aagcgcggag ccgccgctgg
     1861 acaaactcta cgactacact tacgcggacg ctgtcgaggc gggcgtgtcg ccggcaagaa
     1921 aagcgtcgtc cggaagaaaa cggtgagaga gatcacctac cgccaggcgt tgaacgaagc
     1981 gcttgccgag gaaatcgcgc gcgatccaaa cgtctttctg atgggcgagg aagtggccga
     2041 gtacaacggc gcctacaaag tgagccaggg cttgctcgat cgtttcggtg ccgaacgcat
     2101 catcgacaca ccgatcagcg aaaacggttt tgccggcctc gggatcggcg cggcgatggt
     2161 tggcctccgg ccgatcgtcg agttcatgac ttttagcttt tcgttagtcg cgtttgacca
     2221 gatcgtgaac aacgcgccga acatgctcac catgtcgggc ggtcagttta acatcccgat
     2281 cacatttcgc ggaccgaacg gcccggcgca tcaactcggt gcgacccatt ctcacgccac
     2341 cgaatgtttt tatgccaacg tgccgggctt gaaaatctgc acgcccgcca cgccgcgcga
     2401 cgcgaaaggt ctgctcaaaa cggccgtgcg cgacaacaat ccggtcctcg tcctggagtg
     2461 cgagcttttt tacagccaca agggaatggt ggaagacgac ggtgtcgatc ttttggttcc
     2521 gctaggcgaa gcggaaatca aacgcgcggg gagcgacgtc acgatcatct gttacgcgca
     2581 gaccgtgccg atggcgctgg ccgcggccga gacgctggcg ggcgagaaaa tcaacgccga
     2641 ggtgcttgat ttgcgctcga tcaagccgct cgatctcccc gcgatcctgc aatctgtcgc
     2701 gaaaacccat cgggtcgtga ttgcggaaca ggaccgtccg ttctgtggcg tcggcgcgga
     2761 aatcacctat cggattcaga aggaaatatt cgacgagctc gatgcgccga tcatgcgcgt
     2821 cgcgcaggaa gacgtgccga tgccctacaa cgagcgtctc gagcaggccg tgctcccgaa
     2881 cgcggcgaaa atcgtcgccg cagtcgaaaa gatttgttag ccgttctgtc acgcgtcacc
     2941 cgccacttcc catatgcctg aaatccaaat gccgaagctg agcgacacca tgactgaggg
     3001 gaccttggtg agctggaaga agaaaatcgg agaacaggtg acggccggcg aagtgctggc
     3061 cgagatcgaa accgacaagg cgacgatgga atgggaagcg accgatgacg gtgtgttaca
     3121 ggagattttc gtcccggaag gcgggaaggc gaacgtaggc gaacgcatcg ctttcatcgg
     3181 cgacggcaac gaaaccgcgc cggaggctga gaaacccgcc ccggcgccgg gaaaagggcc
     3241 ggacaagaag ccggccgcgg aaaccaaggc gaagccggtc gaggcgggcg tgccgcccgc
     3301 tcccgaacaa ccggtcgggc ggatcaaagc ctcccccctc gcacgcaaga tcgcggagga
     3361 aaagaacgtt gacctaacga tcatcaaagg aaccggtccc ggcgcgcgca tcctcgaaaa
     3421 agatgtggag gcgatggtgt tggccgccgg gagaccttcg aaaacgaaaa ctgccgcgcc
     3481 gccgatcagt ccgggagaat cggcgcgcat cagtctcacg gggatgcgta gaattatcgc
     3541 cgagcgcctg gttgagagca aaggaccggt cccgcatttc taccttaaca tcgagatcga
     3601 cgccgacgcg ctcatgcggg tccgggccga actgaagtct gccggcgacg acgatgacgc
     3661 gacgaaaatt acggttaacg atttcatctt gaagggcgca gttgtagccg ccttgcgcga
     3721 gccgaaagtg aacgccgcct ttgatggcga tgccgtcatc cattacggcg acatcaacct
     3781 cgcggtcgcg gtcgcgatcg acgaaggcct tgtcacgccg gtcattcgcg cggcgcaaga
     3841 caagtcgctc cgcgagatca gcgccgtagt caaggatttc gcccatcgcg cgcgcaacaa
     3901 gcggatgaag ccggaggaat tccaaggcgg caccctgact gtctcaaacc tcgggagcta
     3961 cgggatcgat gattttcttg cgatcatcaa cccgccgcag gcggtcatcc ttgcgatcgg
     4021 cgcgatcgtg cgcaaaccgg tggtgaacga agcgggcgcg atcgtgcctg gctaccggat
     4081 gagcatcggg atgagctgcg accatcgggt ggtcgatggc gcgatcgggg ccgagtatct
     4141 gaaggcgctc cggcacgtcc tcgaaaatcc gtcgcttctt ttgctttagc gttcttccct
     4201 tgcgccgcgc ggttgcgttc ccaaattgac cggcgaactt gttaggcgca gatgagttcg
     4261 ccacgggata cgccaccgcc taacgagaag aaacttcgcg tcgtttcgtt tgcgatccat
     4321 gcgacccgcg ggttgttgcg cgaccagagc gcgcggcgct ggaccatgtt tgggctggtc
     4381 tgcgccgcgc tcgtgctgct ttcttgcggg gcgacttttc tcgggccggt gctcgatccc
     4441 cgctcccggc cgggctggtt tattttttac tggttcgtct gcgcgtggat caccgcgtcg
     4501 gccgccctcc tggcggtttt tgatctcctc ctggtgcgcg cgcgggcccg cgcggtgaag
     4561 cgggcgctgg aggagcagat ttcggcgccg cccaaagcca gcaatgcaga ctaacgaatt
     4621 cgcgcgcggc gccgcgctcc cgcccacggg ctggaaacgg cgcgaacaaa tggaggcgga
     4681 tgagcggcgg acgctcgcgc cctttgccca aaaatcgggc gactcgcgcg gccggcattt
     4741 tcccgaggag cgtcatcttt accggacgga atttcagcgc gaccgcgccc gggtgattca
     4801 ttcgcgcgct tttcgccggc tcgaatacaa gacgcaggtt tttctgaacg ggacagcgga
     4861 tcatttgcgg acgcgcctga cgcatacttt cgaagtggcg tcggtcagcc gcacgctcgc
     4921 gcgcgcgctc gccttgaacg aagatctcac ggaagcgatt gccctggcgc acgatctggg
     4981 gcatgcgccc ttcggacatt cgggcgagga gatgctggcc gaatgcatgc gcgagcatgg
     5041 cgggttcgag cataaccggc agagcttgcg cgtggtcgag ttgctcgaga cggcgtaccc
     5101 ggcgtttccg gggttgaacc tgacttacga ggtgctcgag ggtttgcgga agcatgacga
     5161 gggcgcagcc ccgtcactcg aagcacagat tgcgaatctg gcggacgaga tcacttatta
     5221 cagtcacgat ctggatgacg cgctcgactt tgccattctc gacagcgatc agctcgacga
     5281 gagcgcgatc tggcaaagga gccacgagcg ggtggcgacg cgaagtcccg gtttgttagg
     5341 cacggagctg cacaagaata ttatccgcga catcatcgac ttgcaggtgc gcgatgtggt
     5401 ggcgacgagc gcgacggcga tcgcggcggc cggaccgacg agcgtggagg aggtgcgggg
     5461 acgaaaagag ccgctgattc gctacagcga cgaacagggt gtcgcgaatc gcgacctgcg
     5521 gcgctttctt tacgagaacg tttattatca tccgcgggtg gcggaggtga accggcgcgc
     5581 ctgcgagatg ctgcgagcgg tctttgtggc cttcgtgcgc gagccggaac gattgggaga
     5641 tgccgccacc cggcgaatcg aaggcgaagg gctgcatcgg acagtgtgcg attatattgc
     5701 gggaatgacc gatcgctatt tgctggagga gcatgcgcgg ctggcgaggg gatgacgaaa
     5761 tcggggctcg ttaggggacg ccgccgttct cttcgcgcag aaattggatg agcaggcgca
     5821 ggcgctggtt gaggtccagt tcctcaagca attgttgccg gcgcgcggaa tcgttgatga
     5881 acgtcgaggc gatcaaatcg gcgagcattt ccggatctga cagctcgctg agatagcgat
     5941 cgatcttttc cggcaattcg cgaccgctct ctttaaggtg ggcgtagaac tccaggacct
     6001 tggcggcgag ggcgtcggtt tcgacgcttg gtttagtgcg cgtcttgagc gcctcgatcc
     6061 gcgcgatcgg gaaggggact tcctgttcga actcgctgaa cttcacccgc cgaatgcctt
     6121 gcaggatcag gtcggatgtt ccatcgctcc gcccgacgca ggcgcgaatc agcccgacgg
     6181 tgctcaccgg gaagaagtca gcgctggtcc gccattgttc ccgctccggt cgcacgaggg
     6241 cgacgcaaag catccggtca tcctgcaaag cgtgttcgag catgtcgcga aagcgcggct
     6301 caaagatgtg gagcgggagc agggcgttgg gaaagaggag cacgccggtg aggggcatga
     6361 ccgggacggt ggcgggcagt tcgactgttt cggacatcag gagcgcgtga ttctacctaa
     6421 ttatacgcgc gccggtcgcg tcggagttgg cgcagcgaag gctggcctga gatgtttgct
     6481 tcgccgagcg agacatagat gaggactgaa ccgagccgcg ccagctgccg gcgggaggcg
     6541 aggccgagtt ttccgatccc catcgcggcc atcggcagag gcgtgcgggt tgtctcgaag
     6601 aaggagatga gccgcgccag ctgggccggc gtgtcggtgc gggtggcaac tttgaaaatc
     6661 gcgggcctcg ccgcggcggc taattttgcc tggcggcgca gttcgttggg cggcggtgtg
     6721 tcgcggaggt cgtggcggga aataatgagg ccgattcgac ggcggcgcat ttgggcgatc
     6781 agcgccttca tttgccggag ggagcggaac tccagatcga cgaaggcggc ggacccgaga
     6841 aagcgaagga gcagggcgcg ccgcgcggcg agattgagat tgccgcagcc tccctccgcc
     6901 ggatgacgcg cggtgagaat gagcggagcg cgcaacttcg ggagcacgcg cgccacttct
     6961 cctaacgact ggtaaagcgc gtcgagacgg agttcgaaaa gatcgggcgg gtggcgcaat
     7021 cgcgccgctc gggcaagcgc cgccggtgag gcgatgacac ccactactcg gctgaccgaa
     7081 gcgcggtttg tgaccgatct tttgttgaac atatattgtc ttcgggcgca caacttgcga
     7141 tgccatccga cgccccgaaa gcgaattcat aacatgctcg cccgccaaca ggaattaagc
     7201 acccagttcc agcagctcct cgacgcgttc ctgctggccg gcgcgctttt cgccgcgcac
     7261 gcgctccgtt tttacggcac gacatggctc gaccttccct actcgatcga tccctttcgg
     7321 aactatcaat ggctcctggt cgtgatcatg ccgttcggtc cgatcattct cgatctgcag
     7381 ggattttatc tcgccccgct caacaagacg ctctggcaat cgttcgtcca ggtagcgcgc
     7441 acgatgattt atctcagtat cctggtcagc gggtgcgtta tttttctgcg cctgccgttg
     7501 accagtcgcg cggtcccgct gctcttcatc ctgctcgcga ccgcgatgct cctgatgaag
     7561 cagaggctca tcgttaggcg aatccggcgc cgggccgaac ggggagaggt gcgcgagcgg
     7621 gtcttgctcg ccggggtgcc gcaggacatc gctgcgctcg agcagtctct tacccccgag
     7681 gaaaccatgg tactgcaggt ggccgaacgg atcgatatcg agaaacaacc catctccgac
     7741 ctggtggaag cgatgcatcg acacacggtc tcgcgggtta tttttgccgc cggtcatggc
     7801 cagctcaatc gggtggaaca ggcgatcggc gcctgcgaag tggagggcgt ccccgcctgg
     7861 ttggtggcca attttatcca gacggcgatc gccaagccgg acttcgacgc ctttggcggc
     7921 cggcccatgc tggtctttcg ctcgacgccg gatgtgtcgt gggcgtttcg gctcaagggc
     7981 ttgatcgatc gtgtcggcgc actccttggt ctcatcgtgc tggcggttcc gatgctgatc
     8041 gtcgcgctcg ggattcgcct cacttcgaag ggtccaatta ttttcaagca ggagcgcgca
     8101 gggaaaaacg gcaagccttt cgtcatgtac aaatttcgct cgatgtcgag cgacgccgag
     8161 atgcggcggg cggaattgct gcctttcaat cagatgcgcg ggccagtctt caaggtcgcg
     8221 gacgatccgc ggatcacgtc gcttggccgt tggttgcggc gcacgagcat ggatgaagtg
     8281 ccgcaactct tgaacgtcct gaagggaaac atgagtctgg tcggtccgcg gcccttgcca
     8341 ctttacgagg tggagaaatt tgagaacacg gcgcagcggc ggcggctcag tgtgaagccc
     8401 gggctcacct gtctctggca gatcagtggc cgcaaccagg taatggattt cagcgactgg
     8461 gtgaagcttg atctcgagta catcgaccgc tggtcgttag gcctggattt taaaatcctg
     8521 tgccgcacga tcccggccgt gctcttcggg ctgggcgcaa agtagctggg gacgccgccc
     8581 tcttcggagg cgcggtgtcg gagatccctg ggcaaagact tctcgcttgc aacgctttct
     8641 caggcactgc gcggagcgaa gcgtctacaa gatcctccgc ggcagcatgc gctcgacgta
     8701 tttggcgagg aggtcgaact cgagattgac gagatcgccg gcctgcaggg tttcgaaatt
     8761 tgtctgccgc cgggtgtggg gaatgatcca aacggcgaag ctcgccggga gaagctctgc
     8821 aatcgttagg ctgacgccgt tgagcgcgat cgagcccttg aaggcggcgt agtgggcgaa
     8881 ctcgccgggc aattccactt cgagccgata atcgacgccg tgcggcgcga agctgagagc
     8941 ccgcgccgcg cagtcgctgt ggccctggac aaaatgtccg cccagacgcc cgtcggccgc
     9001 gagagcgcgc tccaggttta ccgggctcgc ggggcggagc gtgcgtagat tggttcgtgc
     9061 gagcgtttct tcgagcaggt cgaaggtcag attacccgcg cgaatcgcgg tcgccgtgag
     9121 gcagcaacca ttcacggaga cgctgtcgcc cacgcgcagg cccggggcga tcaacggtgc
     9181 ggcgagttgc aattgcgtgc cccgttcggt ggcgcgaagc cagcgcacgc tgcccacttc
     9241 ttcgattaat ccagtaaaca tattgcatcc gcagattaag cggattttcg cagattgaat
     9301 ggagagattt ttgcccacgt gccggtcggt gcaggatcag ccgaactcag tttgtctccc
     9361 agaagcgaac cggattgatc cggactggaa agaaggaacg gcgcatctct ggtgtcgcgc
     9421 cggaaccttg tcgggcgcga ccgttgcttt cctgcgcacc acgcaggtgg tcatcaattc
     9481 gctgggagcg ggcctgacgg ccggcttcct cgccaagaaa attcttctcg ctggcgctaa
     9541 aattgctcaa attgctcgtt aggatgaagc aggtggaacg actttccggc ggcgcggcca
     9601 gcagaatgcg acacgcggtg ggcggaaccc gttcaaaaat attttaaaaa aaatgaaaaa
     9661 aacgcttgtc agggctgaaa ttcttcttta atcctcacca ccaaacctaa ccaaacctaa
     9721 ccaaacccaa tggagaaacc aatgataaag aacctaacaa aaacgatcgg agtgcttcgt
     9781 gcggccctcg tggctggcgt cggatttcct tttctgatgg gcgcaagtct gtatgcacag
     9841 gcgcctgcaa caactgcgcc cgcaggcggc gaggccacca ccgagcgcat caccgtcacc
     9901 ggttcgttaa ttccgacagc cgaggaagtg acggcatcgc cgctggatac tctgaatacc
     9961 tcggacatcg ctgtctctgg cgggacggcc gatgttttga ccatcctgca aaagcgcaac
    10021 cccgacttcg tcggcggcgg caaccttggc ggctcgaacg ccaacattgc cagcggcgcg
    10081 acgcagggcg gatccatcat ttcgctgcgt ggcttgccga cgctggtctt gttggagggc
    10141 cggcgcatcg cagactcggc ggcgatctct gcgggtggat tccaattcac cgacgtcagt
    10201 ctcttcccga cttcactcat cagcagaatt gaagtcctga aggacggtgc ttccgccgaa
    10261 tacggatcag aggcggtcgg cggcgtcgtc aatatcttcc tgaaaaaaga ttttgaggga
    10321 gtcgaaattg gggcgcgtta cggcttcagc gtctcgagtg ccgtggcgga gcgcaaagcc
    10381 tacgccattg cgggcgtcgg gaacgacacg acgcacgtga cggccggctt ccagtattat
    10441 gaaatcgatc cgctcttcaa tcgtgagcgg gggtattcgc ggcccttcgt tggcggcacg
    10501 acgaccttcc ccggtggcgg ccgagacgaa gcaggtggcg ccactaattt ctacatcctg
    10561 aaatcgaatc tcaattcgcc ctttgacgca ccgggtgtga cgccgggagg ggttattgtt
    10621 ggttcaaata cgggtgccta ttcacagatt ccgcaggcgt ataacaacgt tccgttcact
    10681 accgcaaacc cgggttttat accgggcggt ttcctcggct tcgacttgtc gaagctaccg
    10741 accagcacaa ttgatatttc caatacgaac gcctacgctt cgtttacgca ccagattttc
    10801 ggaaagcaat tggagatttt cggagatttc ctctacgcgc ataaccacaa cgagagcttt
    10861 ttaaatggcc agccgctgaa ca
//
LOCUS       DATMNS010000115         4774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_879424, whole genome shotgun sequence.
ACCESSION   DATMNS010000115 DATMNS010000000
VERSION     DATMNS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879424"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1731)
                     /gene="nuoF"
                     /locus_tag="VNW28_04285"
     CDS             complement(<1..1731)
                     /gene="nuoF"
                     /locus_tag="VNW28_04285"
                     /inference="COORDINATES: protein motif:HMM:NF010120.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXA09174.1"
                     /translation="MTTNVPPDLERKMDETIGHYPAEQRRSASLPLLHLWQEHFGFIS
                     DAGVNWIARKLGLEPINIFELITFYPMLRRAPAGRKHLRVCRTLSCAMAGAYSLMENL
                     ARLTKIDRERDGAEMPNPVAVSPDGQYSIEFVECLASCGTAPVCMVDDALYENVAPES
                     APELLGGSLSTGTQPPPPHPLERRLVFQNIGREGYTTDIGCYLQNGGYEQLKAAVKMT
                     RAEIVNEVKTSGLRGRGGAGFPCGVKWAFIKPDEKKPVYLICNADESEPGTFKDRYLI
                     HQDPHQVLEGILISCFALNVHTAYIYIRGEFPEGAKTLARAIEEARAHGFLGRNALGI
                     GFDVEIYIHRGAGAYICGEETGLIESLEGKRAYPRIKPPYFPAVLGLYLCPTIVNNVE
                     TLCHVKHIIAMGGAQYAMLGRPNNTGTRIVCVSGDVVRPGYFEIEVGAVTMGQLINEM
                     AGGPKEGRKVKAVIPGGSSAKVLRAGETFKLKDREISFDEIPMDFDTLAGVGSMAGSG
                     GVIVLDDSRDMVWALNNVNEFYAHESCGQCTPCREGSLWMKKITERMLEGGGVIQDPA
                     TLNNVADNIAG"
     gene            complement(1753..2040)
                     /gene="bamE"
                     /locus_tag="VNW28_04290"
     CDS             complement(1753..2040)
                     /gene="bamE"
                     /locus_tag="VNW28_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HXA09175.1"
                     /translation="MKNILRSRLLVLFFAAVFALNACHGQRLTRANVDEVAKGMSRKQ
                     VESILGLPTSVDSTDLLITKKVTYIYRQGNESVTIVFFNDEVASKESNLHE"
     gene            complement(2174..2482)
                     /gene="bamE"
                     /locus_tag="VNW28_04295"
     CDS             complement(2174..2482)
                     /gene="bamE"
                     /locus_tag="VNW28_04295"
                     /inference="COORDINATES: protein motif:HMM:NF016258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HXA09176.1"
                     /translation="MKNKTHLFGLVFAALALLSLGACDRNILSGSKLTSANYDQVTLG
                     MSKAQVETILGPPTSIDSKEMLIFKKTTFRYEDGKKFAIITFKNDEVDSKDTNLGREP
                     "
     gene            complement(2495..3748)
                     /gene="nuoD"
                     /locus_tag="VNW28_04300"
     CDS             complement(2495..3748)
                     /gene="nuoD"
                     /locus_tag="VNW28_04300"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HXA09177.1"
                     /translation="MAGSTREIEAPDPLARTLQAQAEATPGDDFGEKMVLNMGPSHPA
                     THGVLRLELELDGELVTKATPHVGYLHRGDEKIAENMQYNQFVPYTDRLDYLAPLANN
                     VAYALAVEKLMGWELPPRGKAIRVICCETARLSAHLLGLGAMALDLGAMTVFLYTFTE
                     REKLYNLFELLTGARFTTSYTRVGGQIRDLPVTFIPQLERFLDDFIPQLDEIDRLLTR
                     NRIFVDRTRDIGVIPRERAIAYALSGPNLRGSGVEHDLRRKHPYLDYDQYDFEVAIGS
                     AGDCYDRYLVRIEEMRQSVRILRQVIAKLPGGPINVVDWKNMLPPKSQVMTKMEELIH
                     HFIVVTEGLDAPPGEVYFAAENPKGELGFYIHSKGGGVPNRLKIRAPSFVNLSILPEL
                     LPGSMMSDVVAILGSLDFVMGECDR"
     gene            3860..>4774
                     /locus_tag="VNW28_04305"
     CDS             3860..>4774
                     /locus_tag="VNW28_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09178.1"
                     /translation="MSSQQTQRPELSRFSRIMAVALPLLCLATPSFWLMTTVPPLWRD
                     VDAYIQISSSPGPSTILLHGPLYCQLARVPLWIGHRTTGGSMGFAGFMKNPRLTDAGI
                     YLLLSMQHAGLWLAAFYLLSALTASLLVRVALAIFFASHPIFYGFAHCVEPETLSMIL
                     ILLLVGVGLRMVAAYPVVEARWWLLAGFLLWCGILTRHINALLIGLLPLTFILLAFGQ
                     WWRSSRTELQKVTDAPRFAIRQQLRAAGWSVAIGVIALLLASGYTDLLCRKAHILPRS
                     EAGFTFLWRLNFLPTLPTAARDALVERMA"
BASE COUNT          861 a         1432 c         1438 g         1043 t
ORIGIN      
        1 gccggcgatg ttgtccgcca cgttattcag cgtcgccgga tcctggatga cgcctccgcc
       61 ttccagcatg cgctcggtga ttttcttcat ccagagcgac ccttcgcgac acggggtgca
      121 ctggccgcaa ctctcgtgcg cgtagaattc attgacgttg ttcagcgccc agaccatgtc
      181 gcgcgaatca tccagcacga tgacgccgcc ggagcccgcc atcgagccga ccccggccag
      241 agtatcgaaa tccatcggaa tttcatcgaa cgaaatttcg cggtccttta gtttgaaggt
      301 ttcgccggcg cgcaagactt tcgccgagct gccgccggga atgaccgcct tcaccttgcg
      361 cccctctttc ggtccgcccg ccatttcgtt gattaactgc cccatggtga cggcgccgac
      421 ctcaatctca aagtagcccg gccgaacgac atcgccgctg acgcaaacga tgcgcgtgcc
      481 ggtgttgttc ggccggccga gcatggcgta ttgcgcgccg cccatcgcga tgatgtgttt
      541 cacgtggcag agcgtctcga cgttgttcac gatcgtcggg cacaggtaaa gaccgaggac
      601 cgcggggaaa tagggcggct tgatccgcgg ataggctcgc ttgccttcga gcgattcgat
      661 caggcccgtt tcttccccgc agatatacgc gcccgcgccg cgatgaatgt aaatctcgac
      721 gtcgaagccg atgccgagcg cattgcgccc gaggaaaccg tgggcgcggg cttcttcgat
      781 cgcgcgcgcg agagttttgg cgccctcggg aaattcgccg cggatgtaga tgtaagcggt
      841 gtgaacattg agcgcgaaac aactgatcag gatgccctcg agcacctgat gcgggtcctg
      901 atgaatgagg tagcgatcct tgaaagtccc cggctcggat tcgtcggcgt tgcagataag
      961 gtagaccggt tttttctcgt ccggcttgat aaaggcccac ttcaccccgc acggaaaacc
     1021 ggcgccgccg cggccgcgca agccagaggt tttcacttcg ttcacgatct cggcgcgagt
     1081 cattttgacc gcggctttga gctgctcgta accgccgttt tgcagataac aaccgatgtc
     1141 ggtggtgtag ccttcgcgcc cgatgttttg aaaaaccaaa cggcgctcga gcggatgggg
     1201 aggcgggggt tgcgtgcctg ttgacagcga accgccgagc agttccggcg cgctttctgg
     1261 cgcgacgttc tcgtagaggg cgtcgtccac catgcaaacc ggcgcggtgc cgcaactcgc
     1321 gaggcattcg acgaactcga tgctgtattg gccatcggga ctgaccgcga ccggattggg
     1381 catctcggcg ccgtcacgct cccgatcgat tttcgtcagg cgagcgaggt tttccatcag
     1441 cgaataagcg cccgccatcg cgcagctcag ggtccggcaa acgcggagat gcttccgccc
     1501 ggccggtgcc cgccggagca tcgggtagaa cgtgatgagc tcaaaaatgt tgatcggctc
     1561 gagccccagc ttgcgcgcga tccagttcac ccccgcgtcg ctgatgaatc cgaaatgttc
     1621 ctgccacaaa tgaagcagtg gcaaggacgc gctccgtcgc tgctcggccg gataatggcc
     1681 gatcgtctcg tccattttcc gttccagatc gggcggaacg tttgtcgtca tcgtcgcctc
     1741 cacggatgac ggctactcgt gcaggttgct ttccttcgac gccacctcgt cgttgaagaa
     1801 gacgatcgtg accgattcgt ttccctgccg gtaaatgtag gtgacctttt ttgtgatcaa
     1861 caggtcggtg ctgtcgacgg aggtggggag gcccagaatc gattccacct gtttgcggct
     1921 catgcctttg gcgacttcat cgacattcgc gcgcgtcagg cgttgcccgt ggcaggcgtt
     1981 aagggcgaaa acggcggcaa agaacaggac gaggaggcga gatcgcagga tgtttttcat
     2041 cgatgtgaag ttagataacg aatgacgaat gacgaggccc gaaggaatga ccgatgacaa
     2101 agctcgagga tttcgcttcg tcattcggtt cctggtcatt gcttcggcac tcgtcattgc
     2161 agccgcagac tgatcacggt tcgcgcccca aattagtatc cttgctgtcg acctcgtcgt
     2221 tcttgaacgt gatgatcgcg aacttcttgc cgtcctcgta acggaaggtc gttttcttga
     2281 agatgagcat ctcctttgaa tcgatcgagg tcggcggacc gagaatggtt tcgacctgcg
     2341 ccttcgacat tccaagggtc acctggtcgt agttcgcgct cgtcagcttc gagccggaga
     2401 gaatattgcg atcgcaggcg cctaacgaca gcagtgcgag cgcggcaaaa acgagtccga
     2461 agaggtgggt tttattcttc atctttgaag catttcatct gtcgcattcc cccatgacga
     2521 agtccagact gccgaggatt gcgaccacgt cgctcatcat gctgccggga aggagttcgg
     2581 gcagaatgct caggttaacg aaagacgggg cgcgaatctt caatcggttt ggcacgccgc
     2641 cgcctttgct gtggatgtaa aagcccagct cgcctttcgg attctccgcc gcgaaataga
     2701 cttcgccggg gggcgcgtcg agcccctcgg tgacgacgat gaagtggtga atcagctcct
     2761 ccattttcgt catcacctgc gacttcggcg gaagcatgtt tttccaatcg accacgttga
     2821 tcgggccgcc cggcagcttc gcgatcacct ggcgcaggat gcgcacgctc tgccgcattt
     2881 cttcgatccg cacgagatag cgatcgtagc aatcgcccgc gctcccgatc gcgacctcga
     2941 agtcgtattg gtcgtaatcc aaatacggat gcttgcgtcg cagatcgtgc tcgactccgc
     3001 tcccgcgcag gtttgggccg gagagcgcgt aggcgatcgc gcgttctcgc ggaatgacgc
     3061 cgatgtcgcg ggtgcgatcg acgaagatgc gattgcgcgt cagcagacgg tcgatctcgt
     3121 cgagttgcgg aatgaaatcg tccaggaagc gctcgagttg cgggatgaac gtcaccggca
     3181 aatcacgaat ctgcccgccg acccgggtgt aggaagtggt gaagcgagct ccggtcagga
     3241 gctcgaacaa attgtagagc ttctcgcgtt cggtgaaggt gtagaggaaa accgtcatcg
     3301 cgccgagatc gagcgccatc gcgccgagcc cgagaaggtg cgcggaaagg cgcgccgttt
     3361 cgcagcagat aacgcggatc gccttgccgc gcggcggcag ttcccagccc atcagcttct
     3421 cgaccgcgag cgcgtaggcg acgttgttgg ccagcggcgc gagataatcc agccggtccg
     3481 tgtaggggac aaactggttg tattgcatgt tctcggcgat cttttcgtcg ccgcggtgca
     3541 ggtaaccgac atgcggcgtc gcctttgtca ccagttcgcc atccagctcc agctcgaggc
     3601 gcaaaacgcc atgagtcgcc gggtgcgacg ggcccatgtt gagcaccatt ttttccccga
     3661 aatcatcgcc gggcgtcgcc tccgcctgcg cctgcagggt gcgggcgagg ggatcgggcg
     3721 cttcgatttc gcgggtgctt ccagccatac caaattgtcg ttacgctcgc gaccggcaat
     3781 cagcgcgcaa gcgcaaatta cccctttcaa ccgcaggcac gcggtctgat attccgccgc
     3841 cggttctcgg ggaaattgca tgtctagcca gcaaacgcag aggccggagc tttctcgttt
     3901 ctcgaggatc atggcggtgg ccctgccact gctttgcctc gcgacgccat cgttctggct
     3961 gatgaccacc gtcccgccgc tctggcggga tgtcgatgcg tacattcaaa tttcctcgtc
     4021 acccggtccg agcacgatcc tgctccatgg cccgctctac tgccagttgg cgcgcgtgcc
     4081 tttgtggatc gggcatcgga caacgggcgg gtcgatggga ttcgcgggtt tcatgaagaa
     4141 tccgcgcctg accgatgccg gcatctacct tctcctgtcc atgcaacacg ccggactctg
     4201 gctggcggcg ttttatttgc tcagcgctct tacggcatcg ctcctggtgc gggttgcgct
     4261 ggcgattttc ttcgccagtc atcccatatt ctatgggttc gcccattgcg tcgaaccgga
     4321 aacgctgagc atgatcctca tcctgctgct ggtaggagtc gggctgagaa tggtggcggc
     4381 ttacccggtg gtcgaggccc ggtggtggtt gctcgccggg tttcttcttt ggtgcggcat
     4441 tcttacgcgt cacatcaatg ccctgctcat cgggctcctt cccctgacct tcatcctgct
     4501 cgcgtttggc caatggtggc gctcctcgcg gacagagttg caaaaggtta ccgacgcgcc
     4561 tcgatttgca attcggcagc agttgcgcgc tgccggctgg tcggtcgcga tcggcgttat
     4621 cgcgctcctt ctggcgagcg gatacaccga cttgctttgc cggaaggctc acattcttcc
     4681 ccgatcggaa gcgggcttca ctttcttgtg gcgtctcaac tttctgccca cgctgccaac
     4741 cgccgcgcgc gacgctctgg ttgaaaggat ggcg
//
LOCUS       DATMNS010000116         2035 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_167701, whole genome shotgun sequence.
ACCESSION   DATMNS010000116 DATMNS010000000
VERSION     DATMNS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167701"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..750)
                     /locus_tag="VNW28_04310"
     CDS             complement(58..750)
                     /locus_tag="VNW28_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXA09179.1"
                     /translation="MYTLFHHPFCPHSRFIRLVLGEYGLDLRLVEERVWERREAYLAL
                     NPAATTPVLIAEGFPPVPGVGVIAEYLDEAHGLDVGERRLLPISMAERVEVRRLMSWF
                     NDKFFEEASNPLVTERIYKRFMSEENGGGAPAAEVMRAAKVNVRYHLTYIGWLARTRN
                     YLAGDRLTDVDLAAAAHLSAIDYLGDVPWSEDDAAKAWYARVKSRPSFRPLLSEWLAG
                     VPASRTYVDLDF"
     gene            919..1737
                     /locus_tag="VNW28_04315"
     CDS             919..1737
                     /locus_tag="VNW28_04315"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF001389.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXA09180.1"
                     /translation="MDHIMMSDTLRAVILGIIEGVTEFLPVSSTGHLLLAQRFFGLGE
                     GSFWQSFVVLIQLGAILAIVALYFVKLWKVALGMFSNPDDRRFVIGVLVAFLPAVVVG
                     LIAGKYIKEFLFNPWVVCFSLIVGGAILMWVDQLDLKPREHDATKFPLLTYFWIGVAQ
                     CLAMIPGVSRSGASIVAAMLLGADKRSAAEFSFFLAIPTMVGAFAYDFYKNRGEMTMD
                     HLGIVAIGFMVSFITAMIVVKTFLNYVTRHGFTFFAWWRVIVGTLGLIALAMGR"
     gene            complement(1848..>2035)
                     /locus_tag="VNW28_04320"
     CDS             complement(1848..>2035)
                     /locus_tag="VNW28_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09181.1"
                     /translation="PLKLTPDQVELLQSDNVVSDAAKAAGLTLEGLGITPDSLEAIAP
                     QYLWRFRAAGQFQKKSA"
BASE COUNT          381 a          619 c          619 g          416 t
ORIGIN      
        1 tggcttgcag agcaagcgcg ctcttcagat cggcaggaga aagcgtgacg tgtgcgttca
       61 gaagtcgagg tccacgtaag tccgcgacgc cggcacgccc gccagccatt cgctcagcag
      121 cggacggaac gacgggcggg acttcacccg cgcgtaccac gcctttgctg cgtcgtcctc
      181 gctccatggc acgtcgccca ggtaatcgat cgccgaaaga tgcgctgcgg cggcgagatc
      241 cacgtcggtc agccggtcgc cggcgagata gttccgcgtc cgcgccagcc agccgatata
      301 ggtcagatga tagcgcacat tgaccttcgc cgcgcgcatc acttccgcgg ccggcgcgcc
      361 gccgccgttc tcctcgctca taaagcgctt gtagatccgc tcggtgacca gcggattgga
      421 agcttcctcg aagaacttgt cgttgaacca cgacatcagc cggcgcacct cgacgcgctc
      481 cgccatggaa atcggcagca gccgccgctc gccgacatca aggccatgcg cttcgtcgag
      541 gtattccgcg atgactccga cacccggaac cgggggaaaa ccctcggcga tcaggaccgg
      601 cgtggtggcc gcgggattga gcgcgagata ggcctcgcgg cgctcccaca cccgttcttc
      661 caccaggcgc aggtcgaggc cgtattcgcc gagaaccagg cgaataaacc gcgaatgcgg
      721 gcagaacgga tgatggaaca gcgtgtacat gaagtccttg gtaggtgcgc ggcgcttaaa
      781 aaaacatcaa cccgtcaaag cgacgtcgga ttcgggacac tagccagcca aacgaatgag
      841 gcaacctatg tttggctttt ttttgcgatt gcagcaaaac ggggaatagg cgagaagagc
      901 gcggatttta gcgtcaaaat ggaccacatc atgatgtctg acacgttgcg ggcggtgatt
      961 ctcggcatca ttgagggcgt caccgaattc ctgccggttt cctcgaccgg ccacttgctg
     1021 ctggcgcaac gcttcttcgg tctcggcgag ggcagtttct ggcaaagttt cgtggtgctg
     1081 atccagctcg gcgcgatcct ggccatcgtc gcgctgtatt tcgtcaaatt gtggaaggtc
     1141 gcgctcggga tgttctccaa tcccgacgac cgccgattcg ttatcggcgt gctggtggca
     1201 ttcctgcccg cggtcgtcgt tgggttgatt gccgggaaat acatcaagga atttctcttc
     1261 aatccgtggg tggtttgctt ttcgctgatc gtcggaggcg caatcctgat gtgggtcgat
     1321 cagctcgacc tcaagccgcg tgaacacgat gcaaccaagt ttccgctgct gacgtatttt
     1381 tggatcgggg tggcgcaatg cctggccatg atccccggcg tgtcgcgctc cggtgccagc
     1441 atcgtggcgg cgatgctgct aggagccgac aagcgctcgg cggcggagtt ttcctttttc
     1501 ctggcgatcc cgaccatggt cggcgcgttc gcctatgatt tctacaagaa ccgcggcgag
     1561 atgacgatgg accatctcgg tatcgtcgcg atcggattca tggtctcgtt cattaccgcg
     1621 atgatcgtgg tgaagacgtt cctgaattac gtcacccgcc acggcttcac gtttttcgcc
     1681 tggtggcgcg tgatcgtcgg cacgctaggc ctgatcgcgc tggcgatggg gagataggaa
     1741 caggctatcc tcgtcgttgc gaaagcaggg gccgaaaacc acagatcccc gttggcatgc
     1801 cgcgccacgg cgccccgcta gcccgggacg acagctattt aatccagcta cgcgctcttc
     1861 ttctggaact gtcccgcggc gcggaaacgc cagagatatt gcggggcgat tgcttccagc
     1921 gaatccggcg tgatcccgag accctccagc gtcaatccgg cagcttttgc cgcgtcggac
     1981 accacattgt ccgactgcag cagctcgacc tgatccggcg tcagcttcag cggtc
//
LOCUS       DATMNS010000117         5370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5175021, whole genome shotgun sequence.
ACCESSION   DATMNS010000117 DATMNS010000000
VERSION     DATMNS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5370
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5175021"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VNW28_04325"
     CDS             <1..502
                     /locus_tag="VNW28_04325"
                     /inference="COORDINATES: protein motif:HMM:NF019242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HXA09182.1"
                     /translation="VHASCGCTTAALAKDVVAPNESGEIVATFNIGDRSGVQKKTITV
                     MTDEAASQPTILSLTATIPQLLHLAPTFIFWSANDVLSPKTITAEVGDFPVTKLKVTS
                     TDKSIATEVASGPKEKTFRIIVTPTESGRPINAALKIEPDFPKEAPKSFYVNVRVDSR
                     AKPVPK"
     gene            506..934
                     /locus_tag="VNW28_04330"
     CDS             506..934
                     /locus_tag="VNW28_04330"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXA09183.1"
                     /translation="MPVSIVRQVLLLLGLAFLPAIGQAVYYRGATSWQHPPVDSAPVS
                     VAQAKDWGDAALWIDARAKEQFDGGHVPGAMLLNEDDWNTLLPKVLAVWSPGRKLVVY
                     CSRQTCNASHEVAERLRNEAGLKNVFVLQGGWEEWQKNNP"
     gene            931..1389
                     /locus_tag="VNW28_04335"
     CDS             931..1389
                     /locus_tag="VNW28_04335"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HXA09184.1"
                     /translation="MTKDRDQVQRSNWRLLVRRTLAVLAGAVFVYAGILKARDPVQFA
                     SAINNYQMIPWSVGVRFAFYLPWLEILCGLALIFHRLVAGALAITIGLMLVFVGATLW
                     ARAHGIDVACGCFGSASSNLTLTWHLVLDGCILTALIFLWFAHERPVPVG"
     gene            complement(1433..3397)
                     /locus_tag="VNW28_04340"
     CDS             complement(1433..3397)
                     /locus_tag="VNW28_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA09185.1"
                     /translation="MPTNPPTPDSENLPRGILLIEDYSALGVAISSALHKFAPLHGVQ
                     VARTFAEGEAAAAAMRPELFVIDLDPPPLGDVEFFNKLKAQYPDARVLAIAAGTSHEL
                     RAERGTAGAIQFIEKPFDLAEFGAAVQALLGPWGLPPSTHCRGTLRDLHLVDIVQLKC
                     LALSTAVIRLETADGKLGEIFFQGGKIRHAATGVTRGLDALEDIFRWKGATLSEAELT
                     GDCPRTIDVAWPTLLLPFVRQLAEQERKSIGAAAAQSATVAKTGRKILVIDDTEMLLV
                     FVADVLATADQTFQIITAPTGAEGLRLAQTARPDLVLLDYCLTDMTGDKVCRAMLENE
                     VTAQIPILMMSGHVTELNRTAEDYENVVASLPKPFLSGELINAVEKLLAKGPLPSTPR
                     PKATPKPTSVVPTPAPKPPPAPPAEARPTPLPNGHGIGATEVAPKPPPPAEVPPPASD
                     APKSKILEQPAWPTPPAPPPTARQAEVSVTFSLQVDSLQITPSLRMEGMRLQPVSSTV
                     AVQMAGRTELHGVPLEAGFRLGTITLRDGHVETVRLIPTRQPPQLPAAGSSFAIGAMS
                     LQPENAHQNIRLTSQANKSMRVRLTAPFALLTVELTAGFEVAAVLLEPRGSTVLVRNG
                     SASVSAPFEIQEVELSGAAELRALIVRALP"
     gene            complement(3506..4741)
                     /locus_tag="VNW28_04345"
     CDS             complement(3506..4741)
                     /locus_tag="VNW28_04345"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09186.1"
                     /translation="MNFREILTMALSSVGANKLRSALTMLGITIGVFSVISVMTAIGA
                     MQASIETGLTLFGSNIFQFAKYPINVRAGGSGQKKYDDRRNIIYREAQRYADLMEGNA
                     QEVCLKVFDDGKQAVYNGVKTTPSLTIVGTNKSFLAANAYTLAYGRNLNDEDVALSRP
                     VLVVGRAIEQRLFPHESPIDRVIKLSGHTFTIVGVMATRGTAFGMSQDDIAVMPITRL
                     FEDFGSVNRTINIATQSSSELDYNRTFDRGVGAMRIARGLKAEEPNDFEIYTNDSLKS
                     AFVSIAGVIRIGAFVISTIALLAAGIGIMNIMLVSVTERTKEIGVRKSIGARNRDILR
                     QFLAEAVFISEAGGILGILLGVLGGDLLALWLNVSLIFPFGWAIAGLLVCSAIGIGFG
                     LYPAYRAASLDPIEALRYE"
     gene            complement(4789..5184)
                     /locus_tag="VNW28_04350"
     CDS             complement(4789..5184)
                     /locus_tag="VNW28_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXA09187.1"
                     /translation="MQLKELTENIIGAAIEVHRALGPGLLESTYEMCLCRELALRELV
                     FERQKAIPVEYKGVKLDCGYRADLIVEGMVLVEVKAVEDLTPVNDAQLLSYLKLGGWS
                     IGLIINFNVLLLKHGIRRKVLNLDEEACL"
BASE COUNT         1028 a         1620 c         1693 g         1029 t
ORIGIN      
        1 ggtccatgct tcgtgtggtt gcaccacagc cgccctggcc aaagacgtcg tcgcgcctaa
       61 cgagtccggc gagatcgtcg ccaccttcaa tattggcgac cggagcggcg tacagaaaaa
      121 gaccattacg gtcatgaccg acgaggcggc gagccagccg accatcctca gcctaacggc
      181 gacaattccg caactcctgc atctcgcgcc caccttcatt ttctggtcgg cgaacgacgt
      241 gctcagcccg aaaacgatta ccgccgaggt gggcgacttt ccggttacca aactaaaggt
      301 cacctcgacc gacaagtcga tcgccacgga agtcgcatcc gggcccaagg aaaagacgtt
      361 ccgcatcatc gtgacgccga ccgaaagtgg ccggccaatc aacgccgcgc tgaaaatcga
      421 accggatttc ccgaaggagg cgccgaaatc gttttacgtt aatgtccggg tggacagccg
      481 ggcgaaaccg gtgccgaaat aatttgtgcc cgtttcgata gtccgacagg tccttttgct
      541 gctgggactg gcctttctcc cggcgatcgg gcaggcggtc tactatcggg gcgcgacctc
      601 ctggcagcac ccaccggtcg actccgcgcc cgtgagcgtg gcgcaggcca aagactgggg
      661 cgacgccgcc ctctggatcg atgcgcgggc gaaagagcag ttcgatggag gacatgtgcc
      721 gggagcgatg ctcttgaacg aagacgattg gaacaccctc ctgccgaagg tgctggcggt
      781 ctggtcgccc gggcgaaaac tggtcgtcta ttgcagccgg cagacttgca acgccagtca
      841 tgaggtggcg gagcgcctcc gaaatgaagc cggcctgaag aatgtttttg tcctgcaggg
      901 cgggtgggaa gaatggcaga aaaacaatcc gtgaccaaag accgcgatca ggttcaacgc
      961 tccaactggc gtctcctcgt taggcggaca ctcgccgtgc tggcgggcgc ggtctttgtt
     1021 tacgcgggaa tattgaaggc gcgcgatccg gtgcaattcg cgagcgcaat caacaattac
     1081 cagatgatcc cctggtcggt cggggtgcgg ttcgcctttt acctgccctg gctggaaatc
     1141 ctttgcgggc tcgcgctcat ctttcatcgg ctcgtggccg gggcgctcgc cattaccatc
     1201 gggttgatgc tcgtttttgt tggcgcgacg ctctgggcca gggcgcacgg cattgatgtc
     1261 gcctgcggct gttttggcag cgcgagcagc aacctgacgc tgacctggca cctggtgctc
     1321 gatggttgca ttctcaccgc cctcattttc ctttggttcg cgcacgaacg tcccgtcccc
     1381 gtgggttgac cttcgttggc gccggggtcg cccccggctg cgggcagcca cctcagggca
     1441 aagccctaac gatcagggca cggagctcgg ctgccccgct gagttccact tcctggattt
     1501 cgaacggcgc actcacgctc gcgctgccat tgcggacgag gaccgtgctg ccgcggggtt
     1561 ccagcaggac cgcggctact tcaaagcccg cggtcagctc gacggtgagc aaggcgaatg
     1621 gcgcggtcag ccggacgcgc atcgacttat tcgcctgcga tgtgagccga atattctggt
     1681 gtgcgttttc cggttgcaga ctcatggctc cgatcgcgaa ggaacttccg gcggcgggca
     1741 gttgcggggg ctgccgtgtc gggatcagcc ggacggtctc gacgtggccg tcgcggaggg
     1801 tgatggtgcc caggcggaag cctgcctcga ggggcacgcc atggagttct gtccgtccgg
     1861 ccatttgcac cgcgacggtc gagctgaccg gctgcaatct catcccctcc atccgcagcg
     1921 atggcgtgat ttgcagcgaa tcgacctgga gcgaaaacgt gacgcttacc tcggcctgtc
     1981 gcgcggtcgg cggcggagct ggcggcgttg gccacgccgg ctgttcgagg attttggatt
     2041 tcggcgcgtc gctggcaggc ggtggaactt ccgcgggcgg cgggggcttg ggcgcgactt
     2101 ccgtggcgcc tattccatgg ccgttaggca aaggcgttgg ccgggcttcg gctggcggtg
     2161 cgggaggcgg cttcggcgcc ggcgtgggaa cgacggaggt gggtttgggc gtcgccttcg
     2221 ggcgcggggt cgagggcagc ggacctttgg cgagcaattt ctccaccgcg ttgatcaact
     2281 cgccggagag aaacggcttg ggaagcgacg cgaccacatt ctcataatct tccgctgtcc
     2341 tgttcaactc ggtcacatgg ccggacatca tcaggatcgg gatttgcgcc gtcacctcat
     2401 tttcgagcat cgcgcggcac actttgtcgc cggtcatgtc ggtcaggcaa taatcgagca
     2461 gcaccaggtc gggtcgcgcc gtctgcgcga gtctcaaacc ttcggccccg gtcggcgccg
     2521 tgatgatttg gaaggtttga tctgcggtcg cgaggacgtc ggccacgaaa acgagcagca
     2581 tctccgtgtc gtcgatcacg aggattttcc ggccggtttt ggcgaccgtc gcgctttgcg
     2641 cggcggcggc gccaatcgac ttcctctcct gctcggccag ttggcggacg aacggcagga
     2701 gcagggtcgg ccaggcgacg tcgatcgttc gtgggcagtc gccggttagt tcggcctcgc
     2761 tcagggtcgc acccttccaa cgaaaaatat cctcgagcgc gtccaggccc ctcgttaccc
     2821 cggtggccgc atggcggatt ttgccacctt gaaaaaatat ctcaccgagt ttgccgtctg
     2881 ccgtctccag acggataacg gcggtgctga gggcgagaca tttcaattgc acgatgtcga
     2941 ccaaatgcag gtcgcgcagg gtgccccggc aatgggtcga gggcggcagg ccccagggcc
     3001 cgagcagcgc ctgcaccgcg gccccgaact ccgcgagatc gaacggcttt tcgatgaact
     3061 ggatcgcgcc ggcagtcccg cgctcggccc ggagttcgtg cgaggtgccc gccgcgatcg
     3121 ccagcacgcg ggcatcggga tattgcgcct ttagtttgtt gaagaactcc acgtctccga
     3181 gcggcggagg atcgagatcg atcacaaaca actccggccg catggcggcc gcggccgctt
     3241 cgccttccgc aaaggtgcgt gccacctgca cgccatggag cggggcaaat ttgtgcagag
     3301 cagacgaaat cgcgactccc agcgcgctgt aatcctcgat gagcagaatg ccgcgcggga
     3361 ggttttcgga atcgggcgtt ggcggattcg tgggcacgtt agaagcgtgg aaagcgcccg
     3421 aggataggag aaggcgtgta catgtcaaaa caacccaatc gcaagttgca tgcccccggg
     3481 gagggatggt tagggcggcc ggcgcctatt cgtagcggag cgcctcgatc ggatcgaggg
     3541 aagccgcgcg ataggcggga taaaggccga aaccgatccc gattgccgag cagacgagga
     3601 gcccagcgat ggcccagcca aatggaaaaa tgaggctcac gttcagccaa agcgcgagca
     3661 aatcaccccc gagcaccccg agcaggatgc cgagaattcc tcccgcctcg gaaatgaaaa
     3721 ccgcttccgc cagaaactgg cgcaggatgt cgcgattgcg cgccccgatc gatttgcgga
     3781 cgccgatctc tttcgtccgc tcggtcacgc tcaccagcat gatgttcatg atcccaatcc
     3841 cggccgcgag cagagcgatc gtactgatca cgaaagcgcc gatccggatg actccggcga
     3901 tcgaaacaaa ggcgctcttg agcgagtcgt tcgtgtaaat ctcgaagtcg ttaggctcct
     3961 ccgccttcag gccgcgcgcg atgcgcatcg cgccgacgcc gcggtcaaag gtacggttgt
     4021 agtccaactc ggaggaggac tgcgtcgcga tgttgatcgt ccggttgacg gagccgaaat
     4081 cctcgaacag gcgggtaatc ggcatgaccg cgatatcgtc ctggctcatc ccgaaagccg
     4141 tgccgcgggt cgccatcacg ccgacgatgg tgaaggtgtg gccgctgagt ttgatcacgc
     4201 gatcgatcgg cgattcatgc gggaaaagcc gctgctcgat cgcgcggccg accaccagca
     4261 cggggcggct cagcgcgaca tcttcatcat ttaggttgcg gccgtaagcg agggtgtagg
     4321 cgttggccgc gaggaagctt ttgtttgtcc cgacgatcgt caggctggga gtcgttttca
     4381 cgccgttata aaccgcctgc ttgccgtcgt cgaaaacctt caggcaaact tcctgcgcat
     4441 tgccctccat caggtcggca taacgttgcg cttcgcggta aataatattg cgccggtcgt
     4501 cgtatttctt ttggccgctc ccgccggcgc ggacgttaat cgggtatttc gcgaactgga
     4561 aaatgttgga gccgaaaagc gtcaggccgg tttcaattga tgcctgcatc gctccgatcg
     4621 cggtcatcac cgagatgacg gagaagacgc cgatcgtgat cccgagcatg gtgagagcgg
     4681 aacgcagctt gttcgccccg acggaggaaa gcgccatcgt taggatctca cgaaagttca
     4741 tgggaaatta accgcagagt cgcagagaac gcagagaagg cagacgcgtc aaaggcaggc
     4801 ttcttcatcg agattcaaaa cctttcgccg gatgccgtgc ttcaggagga ggacgttgaa
     4861 attaataatg aggccgatgc tccatccccc gagcttcaga taactcaaga gctgcgcatc
     4921 gttgaccgga gtcaggtcct cgactgcctt gacttcaacg aggaccatcc cctcgacaat
     4981 caagtcagcc cgatagccgc aatctagttt cacgcccttg tattcgaccg ggatggcctt
     5041 ctgccgttcg aacacgagtt cgcgcaacgc gagctcacgg cacaggcaca tttcataggt
     5101 cgattcgagc agtcccggcc caagtgcgcg atgcacctct atcgcagcgc cgatgatgtt
     5161 ctccgtaagc tctttcagtt gcaccctgaa ttcctctgcg tcctctgcgc tctctgcggt
     5221 taaaccattc cgctattcat accttaaggc cgtgaccgga tcgagccgcg aggccgacca
     5281 cgccggcgcg atgcccgaga ccagtccggt caggacagag acgatcatgc tcgcgatgac
     5341 cagaccgaac gaaaaacgaa tcggaaagga
//
LOCUS       DATMNS010000118         3774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5178639, whole genome shotgun sequence.
ACCESSION   DATMNS010000118 DATMNS010000000
VERSION     DATMNS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5178639"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            384..1421
                     /gene="ribD"
                     /locus_tag="VNW28_04355"
     CDS             384..1421
                     /gene="ribD"
                     /locus_tag="VNW28_04355"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HXA09188.1"
                     /translation="MADPTDETFMWAALAEARRGVGHASPNPAVGAVLVQGGRITATG
                     YHRASGLPHAEIECLAAAPGRSLRSATLYVTLEPCSTTGRTPPCTKAIVRAGVGRVVI
                     GAMDPNPRHRGRGMRELMAARIDVTADVLSPECSRLNEAFNKWIVTGQPFVIAKCGMS
                     LDGRLTRRPDESRWLTSPAARQHARRLRAQVDAILIGAETLRQDNPRLTARGVRRARQ
                     PWRVVLSRSGKLPRAAHLFADRFAGRTLVYRGKTLARVLADLGRREITSVLIEGGGNV
                     LGQAFDAHLVDKVQFYVAPLLTGGPVPAVGGKGAESTVAGAHFTDLHYEKIGRDLTII
                     GYPKWAEENAE"
     gene            1476..1760
                     /locus_tag="VNW28_04360"
     CDS             1476..1760
                     /locus_tag="VNW28_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09189.1"
                     /translation="MSWKKLLLIAAVAGAFTFASAARSEAGVSVGIGIGFPIGYPYAG
                     YYPYGYGYPYPYAYPGYYGYYGYGPGFYRGPFVSRRAVAFHNGHRVIHHH"
     gene            complement(1757..3484)
                     /gene="rpoD"
                     /locus_tag="VNW28_04365"
     CDS             complement(1757..3484)
                     /gene="rpoD"
                     /locus_tag="VNW28_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015506105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXA09190.1"
                     /translation="MSLAPPARKRTLDNHNGEFAGSNGDSPPAPTTSTTQARIRELIK
                     LAKEQGYLTFDDLNEALPPDLTDADELDLILIRLRKLEIDIIEASEVDRYKDVKKDSD
                     EDEEKAEPKLDILDDPVRMYLKQMGQVPLLTREQEVEISKRIEEAEEMVAGHINRFGF
                     VAPAHLELAAKLVEGGERFDRVILDKKIESRERYMKMLPRLCKKVQKMSVVLARDYER
                     LTRGSQRDAAKLKKFEKAVARLQSLYPQFFFKQRVTEEFVHLADEHYALLRSIQEEMK
                     KRPKKRGHNLHAKRLREVEMTLWLSITEFYARYQELRGWLRKALRAKTEMIEANLRLV
                     ISIAKKYTNRGLSFLDLIQEGNMGLMKAVEKFEYRRGYKFSTYATWWIRQAITRSIAD
                     QARTIRIPVHMIETINKLMRVQKQLLQEYGREPTPDEVAEEILLPVDRVRAVLKMAQQ
                     PISLQAPVGESEETNFGDFIEDKGAENPSDMTAIALLKEKIKDVLETLTDRERQVLEQ
                     RFGLVDGYSRTLEEVGRQFRVTRERIRQIEAKALRKMRHPTRIRQLEGFLEAADLQTA
                     TTPPVKSVT"
BASE COUNT          676 a         1099 c         1121 g          878 t
ORIGIN      
        1 gaagaggctg ttcatctcgt ctcggagatc agcccatcga gcaaacgcgg gccaggttga
       61 aatttcggga gattggtaac gaattaggct cataatgatt tatcccctct actaaactgt
      121 ctgttcagtg gttgcttagc cagctcatcg gatgccacgc gctgagttct cgatattttt
      181 cgagttaagt attttaatat gaatatatta cagatttatc taatcgcact tgacccgatg
      241 ttaatcgcgc cataacggcg caattagata gccgctcggg tatcggtaat cgtgccagaa
      301 ggttacttct caacggttgt tccaacagcg aatgaaatgc ggaatccatt gcgatctgcg
      361 atccagctgc ttagggttcg gcgatggcag atcccacgga cgagactttc atgtgggcgg
      421 cgcttgccga ggcgcggcgc ggcgtcgggc acgccagccc caacccggcg gtgggcgcgg
      481 tgctcgtgca gggaggtcgc atcacggcca ccggctatca tcgggcgagc ggtttgccgc
      541 acgcagagat cgagtgcctt gcggctgccc ccgggcgctc gctgcggtcg gccaccctct
      601 acgtcacgct tgagccctgc tcgacgacgg gacgcactcc tccatgcacc aaggctatcg
      661 ttagggcggg agttggtcgt gttgtcatcg gggcaatgga tccaaacccg cggcaccgcg
      721 ggcgcgggat gcgcgagctt atggctgccc gaatcgacgt gacagccgac gttttgtccc
      781 ccgaatgcag ccgactgaac gaagcgttta acaagtggat cgtgacggga cagccgtttg
      841 tgatcgcgaa atgcgggatg agcctggacg gtcgcctaac gaggcggcct gacgaaagtc
      901 gctggctcac cagtccggcc gcccgccagc acgcgcggcg gctccgggcg caagtggacg
      961 cgatcctgat cggggcggaa acattgcgtc aggacaatcc gcgcctcacg gcgcgaggcg
     1021 tgcgccgcgc ccggcaaccg tggcgggtgg tcctttcgcg ttctggaaag ttgccccgcg
     1081 cggctcatct tttcgccgat cgcttcgccg ggcgcactct cgtgtatcga gggaaaacac
     1141 tcgcccgcgt cctggcggat ctcggtcgca gagagataac cagcgttctg atcgaagggg
     1201 gcgggaacgt gctggggcag gccttcgacg cgcacctggt cgataaggtg caattttatg
     1261 tcgcgccgtt actgaccggc ggtccggtcc cggccgtcgg gggaaaaggc gcggagtcga
     1321 ccgtcgcggg cgcgcacttc acggatctgc attacgaaaa aatcggccgc gatttgacga
     1381 tcatcggcta tccaaaatgg gccgaagaaa atgctgaata gtttgcgcgc tcccgcgtct
     1441 gaatttacgt taatcaatga aaagggggtg agacaatgag ttggaaaaag cttctcttga
     1501 tcgcggccgt cgccggggcc tttacttttg cttctgccgc gaggtccgag gccggagtga
     1561 gtgtggggat cggaattggt ttcccgatcg gttatccata tgcgggatat tacccatatg
     1621 gctacggtta tccgtatcct tatgcctatc cgggctatta cgggtattac ggatacggtc
     1681 ccggatttta tcgcgggccg ttcgtttcac gacgcgccgt agcgttccac aacgggcacc
     1741 gcgtcattca tcaccattag gtaacggact tgaccggcgg cgtggtggca gtttggagat
     1801 cggcagcttc gaggaaaccc tcgagctgcc gaattctcgt cgggtgacgc atcttgcgca
     1861 gggctttggc ctcgatctgg cggatgcgct cgcgggtgac gcgaaattgc cggcccacct
     1921 cttccagggt gcggctgtag ccatcgacca atccaaatcg ttgttcgagc acctgtcgtt
     1981 cgcgatcggt cagcgtctcg aggacgtctt tgattttttc tttcaagagc gcgatcgcgg
     2041 tcatgtcgct cggattttcc gcgcccttgt cctcgatgaa atcgccgaaa ttcgtttcct
     2101 cgctctcgcc cacgggcgcc tggagtgaga ttggctgttg cgccattttc agcaccgcgc
     2161 gcacccgatc gaccgggagg agaatctcct cggccacttc gtccggcgtc ggttcgcgcc
     2221 cgtattcctg gagcagttgt ttctgcaccc gcatcagctt attgatcgtc tcgatcatgt
     2281 gcaccggaat gcgaatggtg cgcgcctggt cggcgatcga ccgcgtgatg gcctgccgga
     2341 tccaccacgt cgcgtaggtc gaaaatttat aaccacggcg gtattcgaat ttctccaccg
     2401 ccttcatcag gcccatgttg ccctcctgga tcaggtcgag aaacgagaga ccgcggttcg
     2461 tgtatttttt cgcgatcgaa ataacaaggc gcaagttggc ctcgatcatt tccgttttgg
     2521 cccggagcgc tttgcgcagc cagccgcgca attcctggta gcgcgcgtaa aattcggtga
     2581 tcgagagcca gagcgtcatt tccacttccc gcaatctttt tgcatgcaga ttatggccgc
     2641 gctttttcgg acgctttttc atctcctcct gaatgctgcg caggagcgcg taatgctcgt
     2701 ccgcgaggtg cacgaattct tcggtcactc gctgcttgaa gaaaaactgc ggatagaggc
     2761 tctgcagtct ggcgacggct ttctcgaact tcttcagctt ggcggcgtcg cgctgactcc
     2821 cgcgcgtcag gcgctcatag tcgcgcgcca gcaccacgct catcttctgc actttcttgc
     2881 aaagccgcgg gagcatcttc atgtagcgct cgcggctttc gattttcttg tcgagaatca
     2941 cacgatcgaa acgctcgccg ccttcgacca gtttcgccgc caattcaaga tgcgccggcg
     3001 caacgaatcc gaagcgattg atgtgtcccg caaccatctc ttcggcttcc tcgatgcgct
     3061 tggagatttc gacttcctgc tctcgcgtca gcagcggcac ctggcccatc tgcttcaaat
     3121 acatccgcac cggatcgtcg aggatgtcga gcttcggctc cgctttttcc tcgtcctcgt
     3181 cggaatcctt tttgacgtcc ttgtaacgat ccacctccga cgcctcaatg atgtcgatct
     3241 cgagcttgcg gaggcgaatt aggatgaggt cgagctcgtc cgcgtccgtc aggtccggcg
     3301 gcaaggcctc gttgagatca tcgaaggtga gatagccttg ttccttggcc agcttgatca
     3361 gctcccgaat acgagcctgg gtggtgctcg tcgtcggggc cgggggcgaa tcgccgttgc
     3421 ttccggcaaa ctcgccgttg tgattatcca gcgtccgttt cctggccggc ggcgcgaggg
     3481 acaccttttt cccgggtgga cgaccgcgtc cacgcttgag cggtggggcc acgtttgtca
     3541 ccagcttccg cggacggccg cgaggccgtt ttacttgggc aacgactttt ttagtacgcg
     3601 ctttctccgc cttggacttg ggcggacgac cacgcttctt gggcttgcgc gcagtggatt
     3661 tgatcggctt tttggggcgg ccggatgttt tcaatttctt ggccaaaacg aagtgcggtt
     3721 tagcaaataa acgaggtagc aagatctgcg ctagccacgg cccgttaccc tcct
//
LOCUS       DATMNS010000119        11153 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3306364, whole genome shotgun sequence.
ACCESSION   DATMNS010000119 DATMNS010000000
VERSION     DATMNS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 11153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11153
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3306364"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..373
                     /locus_tag="VNW28_04370"
     CDS             <1..373
                     /locus_tag="VNW28_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HXA09191.1"
                     /translation="ITVADFQVAGGLLLLTFAVRDLLELGGDKKPTNEDFGVVPLGMP
                     LIAGPALLTALLVLTDSVGATFTLLSLILNLLIVAVVFCYADRLTKWLGKQGIKGISK
                     LVSLLLAAIAVSLIRRGWQGR"
     gene            complement(382..765)
                     /locus_tag="VNW28_04375"
     CDS             complement(382..765)
                     /locus_tag="VNW28_04375"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HXA09192.1"
                     /translation="MALIPEAKRNVAVLGASPKPERYSNQAMRLLASFDYRPIPVNPT
                     YDEVEGATCFPRLADIGEPVHTVTVYLDPSRSAPLIDEILAANPQRIIMNPGAENEEL
                     AAAASGAGIEVVEGCTLVMLRSGLF"
     gene            863..2152
                     /gene="hpnH"
                     /locus_tag="VNW28_04380"
     CDS             863..2152
                     /gene="hpnH"
                     /locus_tag="VNW28_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HXA09193.1"
                     /translation="MRFPLALTTKIAAYIVGQKIRGTKRFATVLQLEPLHTCNLTCTG
                     CGRIREYSTSLKDILPLEDCLSAAVECNAPMVSICGGEPLIYPQIEALVQGLHKQGRI
                     IYICTNAMFMRKKMREWMASEYPKRAAFIDEKIATLLAANLINEKDAAAIRQGPKDAS
                     KPTIGPSRWMYWNVHLDGLERTHDLIVEREGVFKECILAIKMAKQLGYQVATNTTIYK
                     ETDMGEVEQMFDYLSDLGVDGHTISPGYEYDAAKKDMIKRLNLQPENFFLTRKMTIEK
                     FKKIEQWMQDYTLFGTPIYFEFLAGKRDLTCSAWAIPTRNIRGWKGPCYLMTDAHYSS
                     YQELLDKVQWDKYGVENGVVKDSRCENCMVHCGYEPTASLGLNAQRGDTWKTVRFNFG
                     PKPKPTGKGNEITAYNGVTSGNGHLTGKKAEPAAQAS"
     gene            2162..4165
                     /gene="shc"
                     /locus_tag="VNW28_04385"
     CDS             2162..4165
                     /gene="shc"
                     /locus_tag="VNW28_04385"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HXA09194.1"
                     /translation="MGNSSANIIALPRISAKEGTPRLATSQELAEAISLAQQNLLRQQ
                     KPDGHWVGELMVDSTLCSDYVLFMHWLGEVNEAIQRRCVKHIRNRQLPDGGWNIYFGG
                     PSEINACVKAYFALKLAGHSPEAPFMQEARANILRLGGIPRMNTYSKLYLALLGQFPW
                     KYLPAIPVEMVLLPNWCFFNIYKMSSWSRAMLIPLAIINHFKPTRELPENKQLHELYP
                     VGTEHNDFTLPRDQRLLTWRNFFLRADRALKILERWHSRPLRQRALEEAERWMVERIG
                     EGSDGLAAVFPAMLNSIIALRALDYPKRHPLYQKALQDFSGLFVDDPEDFRIQPCLSP
                     VWDTAINIIALAESGFPAQHPALQRAAKWLRDKEVRIRCDWTVNNPHPEASGWAFEYN
                     NVYYPDTDDTAMVLMALRLVRPNEQAELAEIFERALAWQLSFQCRDGGWAAFDKDVTR
                     HWLEDMPFADHNAILDPTCSDLTARTLELLGYIGFNPGRLLVRRAVRYLRATQEDDGS
                     WYGRWGVNYIYGTWQVLRGLRAIGEDMTADWILRGRDWLESCQNDDGGWGESCASYEN
                     PDLKGQGASTASQTAWALMGLCACGDLDRASVQRGLRYLLSTQREDGAWDELEITGTG
                     FPRVFYLKYDMYRQNFPLLALATYVNYRSGIGHYQSFYRWFGM"
     gene            complement(4332..4664)
                     /locus_tag="VNW28_04390"
     CDS             complement(4332..4664)
                     /locus_tag="VNW28_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA09195.1"
                     /translation="MESVEDIFDDANGDLAIGELEGAVEKYRRCVALDPQFVDGWHAL
                     GMALMKLGRFPEAIEAGKRTVELKPNDQIGWTSLSLFYNRNGEIKEAEAAGTKAKILS
                     WGGKIAPE"
     gene            complement(4709..6253)
                     /locus_tag="VNW28_04395"
     CDS             complement(4709..6253)
                     /locus_tag="VNW28_04395"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXA09196.1"
                     /translation="MITVMRKHHKVLMIIITALVCISFSWYWNKTDFSQLGNATVGKI
                     YDRGVSQLEFQRNARLLRLSSQLGMNDLIQHLAAGAQTEAQAYENFSWNLMVLRHEAE
                     QLGIQPTTAEIADAVKALPTFGGEKGFDLARYTEFVDRALAPMGFSEAQIEELATDQI
                     ALDRVKKILSAGVSVPESEMRKDYEKAYAKMDVSVVRFRPEDFAKDAPVEDTEIAKYF
                     ESHKAQLKTDEKRKVKLVQFGLADEQKKLKGKERIDVLQKLADKANDFTEALQAKGAD
                     FDQVAAKFQITPRETGYFPMASPDRQLAGTPQLAPAAFALGKESPNSDAIQTADGFVI
                     EHLVSVEASRPRTLAEAQPEIVKALKEQMVQQIVAQKATEIAHKLQDELKNGKPLAEA
                     ATQAGVKLEKLPPFALSDEPPGATPAPETKPKNEAPDMPTIKQTASSLSPGGVSDYVS
                     APDGGFIAVLEKREALTAAQFDAARPALESRALLNQNQVVFYEWLRERRHAAGVAEAK
                     PQAAPG"
     gene            complement(6385..7800)
                     /locus_tag="VNW28_04400"
     CDS             complement(6385..7800)
                     /locus_tag="VNW28_04400"
                     /inference="COORDINATES: protein motif:HMM:NF015119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome accessory factor PafA2 family
                     protein"
                     /protein_id="HXA09197.1"
                     /translation="MDRIAGIETEYGCLVSGDAAHPNGDAWPVRVKNHLFRKMRAGAI
                     DLHYRDYEEPPGNGGFLLNGGRIYLDMGHIEYASPECRQLRDVAAYDLAGDQLLQSAL
                     DSLGAAGAVSFIKNNVDHHTGATFGCHENYLMKREAQFTPAALGALLAFLATRQIFTG
                     AGRVGQANPLAFDFEMPPAGEMVDFQLSQRADHIVNDIYQWVQFNRAIINARDEPLAD
                     YRKYRRLHLLIGDSNMSPFANALKIGTTACVLTLLEAGLLPNDLTLLDAVQATREISR
                     DSTQRWIVMLENGQTIGALDVQWRLHALAQKHLGGIDQETDWLLESWRYTLDALPHKP
                     EALIGGVDWISKKWLLESFLAAENLSWDDPWLQSLDLEYHNVDPARGLFFALKPAKRI
                     GEWNNLVRQKDATLTPPADTRASGRAHAVASFRKERRPYVINWDSIAGESHDFLVMGD
                     PFKTYVDEIEQFLSQRCGPQS"
     gene            7866..8294
                     /locus_tag="VNW28_04405"
     CDS             7866..8294
                     /locus_tag="VNW28_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HXA09198.1"
                     /translation="MRPKPFPARSPAETVGGLVYFGRMLDKIRTRARGELPADYIPNL
                     GKGFDGRSVRFLGVSYDDVVARVREGGTDEEILQWAFAKGRKPSEEEVEIWNEFMCKC
                     GWKDNISETLVRRKKESGLTERDDIETMFQFIDADEGRAL"
     gene            complement(8391..8543)
                     /locus_tag="VNW28_04410"
     CDS             complement(8391..8543)
                     /locus_tag="VNW28_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09199.1"
                     /translation="MAQAEAARPTRGRMIVSALLVAVVVIITWVVIDYRQKPPPPPNK
                     IGELVR"
     gene            complement(8549..8833)
                     /locus_tag="VNW28_04415"
     CDS             complement(8549..8833)
                     /locus_tag="VNW28_04415"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HXA09200.1"
                     /translation="MAELIKASELKNRLKKIPEWELEKKHIERTFEFDEFSEAIDFVN
                     SVAEVAEDEEHHPDIDIRFNKVRLILSTHSKGGLTDLDFSLAERIDTLSE"
     gene            complement(8912..10054)
                     /locus_tag="VNW28_04420"
     CDS             complement(8912..10054)
                     /locus_tag="VNW28_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09201.1"
                     /translation="MTVFLGCGFAAKYREGGGNFSVPLQWMLGLRRLQQDAVWLELLP
                     ATTDRANDAACIANFQRQLRAHGLAGRYCLLYQDPVSDAHTLAACRCIGLTQRELRAR
                     LAAPNVLLNLSYSLHPPFLFEFERRIFCDLDPGEIFYWMTKLELGQSQHHEFWTIGLN
                     VHGADCRLPKTSLPWRTFYPLADTELLRAQPRPRRPKFTTIGQWYWDGSIEVDGEFPD
                     LSKQHAFAPYLDLPARVPEAKWELAMNIGADPVEANRLTNLGWELAGPHRVARTPARY
                     RRYLASAVAEFTAIKGVDVRWQTGWMSDRAAAFLALGRPVITENTGAGKYLPTTSGVF
                     FVEGAAEAAAAAHQVLRDWTVLSRQARECAVEVFDSAKNLRQILGL"
     gene            10179..10255
                     /locus_tag="VNW28_04425"
     tRNA            10179..10255
                     /locus_tag="VNW28_04425"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10213..10215,aa:Arg,seq:acg)
     gene            complement(10400..>11153)
                     /locus_tag="VNW28_04430"
     CDS             complement(10400..>11153)
                     /locus_tag="VNW28_04430"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXA09202.1"
                     /translation="RESKFARQLRSRKISFYWGRATIVNDNPDKVLTSSGNADTHLAP
                     TLRGVVDRTRRELFLVSPYFVPGKQGVEILAGVRRRGARVVVITNSLAATDGLPVHAK
                     YKLYRKPLLEAGIELYETKPTAGALHEREPDSSRGGTGSGSSSLHAKTFAFDRRIAFV
                     GSYNIDPRSSKLNTEMGVLFDCPAFAKRLPEKTERDIDRNAYRVELVGNHLEWVTREG
                     NNQVRFDSEPETSLWKRIKVSVLSWLPIESLL"
BASE COUNT         2134 a         3385 c         3391 g         2243 t
ORIGIN      
        1 aatcaccgtt gccgatttcc aggtcgcggg cggtttgctt ctcctcacgt ttgcggtgcg
       61 cgatttgctc gagctcgggg gcgacaaaaa gcccacgaac gaagacttcg gagtggtgcc
      121 gctcgggatg cccctgattg cggggccggc cctgctgacc gcgctcttgg tcttgaccga
      181 ttcagtgggc gcgaccttca cgttgctctc gctgatcctg aaccttttga tcgtagcggt
      241 ggtcttctgt tacgcggacc ggctgacgaa atggctgggc aaacagggaa tcaaaggcat
      301 ctcgaaactg gtttcgctcc tgcttgcagc gatcgcggtc agcctgatcc ggcgcggctg
      361 gcagggacga tagcggacgg atcaaaacag cccgctgcgc aacattacga gggtgcagcc
      421 ttcgaccact tcgatcccgg cgccgcttgc cgccgcggcc agttcctcgt tttccgcgcc
      481 ggggttcatg atgatccgtt gtgggttcgc ggccaggatt tcatcgatca acggcgccga
      541 acgggacgga tcgaggtaga ccgtgacggt atgcaccggc tcgccgatgt ccgccagacg
      601 cgggaagcag gtcgcgcctt caacctcgtc gtaggtcggg tttaccggga tcggccggta
      661 atcgaagctg gccagaagcc gcatggcctg gttggagtaa cgctccggct tgggggaggc
      721 gccgagcaca gcgacgtttc tttttgcctc ggggatgagc gccataggta aggaaattgt
      781 ctcaaaaccg tcgaactgtc ggctggactg atgccgcccg ctccattgac agtggtagca
      841 cgcttgttag tatcccgcac ttatgagatt tcctctcgcg ctaacgacga agattgccgc
      901 ttacatcgtc ggccagaaaa tccgtgggac gaagaggttc gccaccgtcc tgcagcttga
      961 gccgctgcac acctgcaacc tgacgtgcac cggctgcggc cggatccgtg aatattccac
     1021 ctcgctcaag gacattctgc ccttggaaga ttgcctctcg gccgcggtcg agtgcaacgc
     1081 cccaatggtc tcgatctgtg gtggcgagcc gttgatttac ccgcaaatcg aagcgctggt
     1141 gcaaggcctg cacaagcagg gccggatcat ttacatctgc accaacgcca tgttcatgcg
     1201 caaaaagatg cgcgaatgga tggcgagcga gtacccgaaa cgggcggcgt tcatcgacga
     1261 aaaaatcgcg actctcctgg cagctaattt aatcaatgag aaggatgcgg cggccattcg
     1321 gcaagggccg aaggacgcct cgaagccgac gatcgggccg agcagatgga tgtactggaa
     1381 cgtccacctc gacggtctcg aacggacgca tgatttgatc gtggaacgcg agggcgtctt
     1441 caaggaatgc atcctcgcaa tcaagatggc gaagcaactc ggctaccagg tcgcgaccaa
     1501 caccacgatc tacaaggaaa ccgacatggg cgaagtcgag cagatgttcg attacctgag
     1561 cgatctcggg gtcgacgggc acaccatttc gcccggttac gaatacgacg cggcgaagaa
     1621 ggacatgatc aagcggctga atttgcagcc ggagaacttc ttcctcaccc gcaagatgac
     1681 gatcgagaaa ttcaagaaga tcgagcaatg gatgcaggat tacaccctct tcgggacgcc
     1741 gatttatttc gagtttctcg cggggaaacg cgatttgacc tgctctgcgt gggcgatccc
     1801 gacgcgcaat atccgcggct ggaaaggccc gtgctatttg atgaccgacg cgcattattc
     1861 cagctaccag gagcttttgg ataaagtgca gtgggacaaa tacggcgtcg aaaacggcgt
     1921 ggtgaaggac agccgttgcg agaattgcat ggtacattgc ggctacgagc cgaccgccag
     1981 cctcgggctc aacgcgcagc gcggtgatac gtggaaaacc gtgcggttca acttcggtcc
     2041 gaagccaaag ccgaccggca aaggcaacga gatcacggcc tacaacggcg tcacctccgg
     2101 caacgggcat cttaccggca agaaggcgga gccagccgcg caagcttcgt gagcgtgaga
     2161 catgggtaat tcctccgcga acatcatcgc gttgccgcgc atttcggcaa aggagggaac
     2221 gccacgtctc gcgaccagtc aggagctggc tgaggccatc tcgctcgccc aacaaaatct
     2281 cctgcgccag caaaagcccg atggccattg ggtcggagag ctgatggtcg atagcacgct
     2341 ctgctccgat tatgtgcttt tcatgcactg gctcggggag gtcaacgagg caatccagcg
     2401 ccgctgcgtc aagcacatcc ggaaccgcca gcttcccgac ggcggctgga acatctactt
     2461 cggcggcccg agcgagatca acgcctgcgt caaagcctac ttcgcgctca agctggccgg
     2521 gcacagcccc gaggcgccct tcatgcagga agcgcgggcc aatatactgc ggctcggcgg
     2581 catcccgcgg atgaacacct acagcaagct ttatttggct ttgctcggcc aattcccctg
     2641 gaaatatctg ccggccattc cggtcgagat ggtgctcctg ccgaactggt gcttcttcaa
     2701 catctacaag atgtcgtcct ggagccgggc catgctcatc ccgctcgcga tcatcaatca
     2761 tttcaaaccg acccgcgaac tgccggaaaa caagcaactg cacgagcttt atccggtcgg
     2821 caccgaacat aatgatttca ccctgccgcg ggaccaacgc ctcctcacct ggcgcaattt
     2881 tttcctgcgc gccgatcggg ccttgaaaat cctcgaacgc tggcacagcc ggccgcttcg
     2941 ccagcgcgcg ctggaggaag cggaacgctg gatggtcgag cggatcgggg aggggagcga
     3001 tgggctggcc gcggttttcc cggcgatgct gaattcgatc atcgcgctgc gcgctctgga
     3061 ctatcccaag aggcatccgc tctaccagaa agcgttgcag gatttttccg gcctttttgt
     3121 cgacgacccg gaggactttc ggattcagcc ctgtctctcg cctgtttggg acaccgcgat
     3181 caacatcatc gcgctcgccg aatccgggtt tcccgcgcag catccggcgc tgcaacgcgc
     3241 ggcaaagtgg ctgcgcgaca aggaggttcg gattcgttgc gactggacgg tcaacaatcc
     3301 gcatccggaa gcgagcggct gggccttcga atacaacaac gtttattatc ccgacaccga
     3361 cgataccgcg atggtcctga tggccttgcg gctcgtccgg cctaacgagc aggcggagtt
     3421 ggcggaaatc ttcgagcgtg cgctcgcctg gcaactcagc ttccaatgcc gcgatggcgg
     3481 ctgggcggcc ttcgacaaag atgtcacgcg gcactggctc gaagacatgc ccttcgccga
     3541 tcacaacgcc attctcgatc cgacctgcag cgacctgacc gcgcggacgc ttgagttgtt
     3601 aggctacatc gggttcaatc cgggccggtt actggtgcgc cgggcggttc gctacctgcg
     3661 cgcgacgcag gaggatgacg gctcgtggta cggtcgctgg ggcgtcaact acatctacgg
     3721 gacgtggcag gtcctgcgcg gcctgcgggc gatcggcgaa gacatgaccg cggattggat
     3781 cctgcgggga cgcgactggc tggagagctg ccagaacgac gacggcggct ggggcgaaag
     3841 ctgcgcttcc tacgaaaacc cggatctcaa aggccagggc gcaagcaccg cgtcgcaaac
     3901 ggcctgggcg ctgatggggc tttgcgcctg tggcgatctc gaccgggcca gcgtgcagcg
     3961 cgggttgcgc tatctgctct cgacgcagcg cgaggacggt gcctgggatg agttggagat
     4021 taccggcacc ggttttccgc gggtcttcta tttgaagtac gacatgtatc gccagaattt
     4081 tccgctgctc gcgctggcga cctacgtcaa ttaccgcagc ggaatcggac actaccagag
     4141 tttttaccgc tggttcggga tgtagagacg ttttgctccg cgaagcgcaa gggcgcacgt
     4201 cgcttgcgat gtagccgttt cgctgtgcga aacgcgtagg ggaataccgc tcgttctgaa
     4261 atgttgccct gcgcctccca cagggaggcg tctacagcag cggattccca aagggaagcg
     4321 cctgcagaca gctattctgg cgcgattttc ccgccccagg agagaatctt cgctttggtc
     4381 ccggccgctt cggcctcctt gatctcgccg ttgcgattgt agaataacga aaggctcgtc
     4441 cagccgatct ggtcgttagg cttgagctcc accgttctct ttccggcctc gatcgcttcc
     4501 ggaaagcgtc caagcttcat caaggccatc ccgagcgcgt gccagccgtc gacaaattgc
     4561 gggtcgagcg cgacgcagcg ccgatacttc tccaccgcgc cctcgagttc gccgatcgcg
     4621 aggtcgccgt tggcgtcgtc gaaaatatcc tcgaccgatt ccatctccct gggaattccc
     4681 atccttccga gcgccgcccg gtcgaaggct agcccggcgc ggcctgcggc ttggcttccg
     4741 cgacccccgc cgcgtgccgc cgttcccgca gccattcgta gaaaacaacc tgattttggt
     4801 tgaggagcgc gcggctctcg agcgccggcc gcgccgcgtc aaactgtgcc gcggtgaggg
     4861 cctcgcgttt ttcgagcacc gctataaaac caccatcggg cgcgctcacg taatcgctca
     4921 cacctccagg gctcaagctg ctcgccgttt gcttgatagt cggcatatcg ggcgcctcgt
     4981 ttttcggctt cgtttccggc gcgggggtcg cgccgggcgg ctcgtcggac aaggcaaacg
     5041 ggggcagttt ttccaacttc acacccgctt gggtcgcggc ttccgccagc ggcttaccat
     5101 tcttcagttc gtcctgcagc ttatgcgcga tttcggtcgc cttctgcgcg acgatctgct
     5161 gcaccatttg ttccttgagc gctttcacga tctccggttg cgcttctgcg agagtgcgcg
     5221 gccgggacgc ctccacgctc accaggtgct caatcacgaa gccgtcggcc gtttggatcg
     5281 catcgctgtt cggcgactct ttgcccaggg caaatgccgc tggcgccaac tgcggcgtcc
     5341 cggccagctg tcgatcaggc gacgccatgg ggaaataacc ggtttccctg ggggtgatct
     5401 ggaacttcgc cgccacctgg tcgaagtcgg cccccttggc ctggagtgct tcggtgaaat
     5461 catttgcttt atccgccagt ttttgcagga catcgattcg ctctttgcct ttcaacttct
     5521 tttgctcatc ggccaggcca aactggacga gcttcacctt gcgtttctca tccgtcttca
     5581 actgtgcctt gtgcgactcg aaatatttcg cgatctcggt gtcctccact ggcgcgtcct
     5641 tggcgaaatc ttcagggcgg aagcgcacca cactcacgtc cattttcgcg taagcctttt
     5701 cgtagtcctt ccgcatttcc gattccggca cgctcacccc ggcgctcaaa attttcttca
     5761 cccggtcgag tgcgatctga tccgtcgcca gctcctcgat ttgcgcctcg ctaaagccca
     5821 ttggcgcgag cgcgcgatcc acgaactcgg tgtagcgcgc gagatcgaat cctttctcgc
     5881 ccccgaaagt gggcagcgcc ttgaccgcat cggcaatctc cgcggtcgtc ggctggatcc
     5941 cgagctgttc tgcctcgtgg cggagcacca tcaggttcca ggagaaattc tcataagcct
     6001 gggcctcggt ttgcgcaccg gccgccaggt gctggatcaa atcgttcatc ccgagctggc
     6061 tgctcagccg gaggaggcgc gcgttgcgct ggaattccag ctgcgaaacg ccgcgatcgt
     6121 agatcttccc gaccgtcgcg ttccccagtt gcgaaaaatc cgtcttgttc cagtaccagg
     6181 agaaggaaat acagacgagg gccgtgatga tgatcatcag gaccttgtgg tgcttgcgca
     6241 ttacggtgat catgggatta agcgggttgg agtggcgaat ctagggtctc gctcccgggc
     6301 ggcaactgat ttttgctttg ttgcgccgat ccgaccgctg aaggacggcc tcaatcgaag
     6361 gggaacggcg ggcggagacc gccactacga ctgcggcccg caccgttgcg agaggaattg
     6421 ctcgatttcg tcgacgtagg tcttgaacgg gtcgcccatc acgagaaaat catggctctc
     6481 accggcgatc gaatcccagt taatcacgta gggccgccgt tctttgcgga aagaggccac
     6541 ggcatgggcc cggccgctgg ctcgcgtatc ggccggcggg gtcagggtgg cgtctttctg
     6601 gcggacgaga ttgttccact cgccgatgcg tttggccggt ttgagcgcga aaaaaaggcc
     6661 gcgggcggga tcaacgttgt gatactcgag gtcgaggctc tggagccacg gatcgtccca
     6721 ggacagattc tccgccgcca ggaacgactc gagcagccac ttcttgctga tccaatcgac
     6781 cccgccgatc aacgcctcgg gtttgtgcgg caaggcatca agggtatagc gccagctctc
     6841 gagcaaccag tcggtttctt gatcgatccc gccgagatgt ttttgcgcca gcgcgtgcag
     6901 acgccattgc acatcgagcg ccccgatcgt ctggccgttc tccagcatta cgatccaacg
     6961 ctgcgtcgaa tcgcgcgaga tttcccgcgt ggcttggacg gcgtcgagca gcgtcaggtc
     7021 gttaggcaaa agaccggcct cgagcagggt caggacacac gcggtcgtcc cgatcttcag
     7081 cgcgttcgca aatgggctca tgttcgagtc gccgatcagc aggtggagcc ggcgatactt
     7141 gcggtagtcg gcgagcggtt cgtcgcgcgc gttaatgatt gcgcggttga actgcaccca
     7201 ttggtaaatg tcgttcacga tatggtcagc ccgctggctg agctggaaat cgaccatctc
     7261 tcccgccggc ggcatctcga aatcaaaggc cagcggattc gcctgcccga cccggcccgc
     7321 gcccgtaaaa atctggcgcg tggccagaaa agcgaggagt gcaccgagcg cggcgggggt
     7381 gaattgggct tcacgcttca tcaaatagtt ctcatggcag ccgaaagtgg cgccggtatg
     7441 gtgatcgacg ttgtttttga tgaacgagac cgcgccggcc gcgcctaacg aatcgagcgc
     7501 ggactgcaga agctggtcgc ccgccaaatc gtaggccgcg acatcgcgca attgccggca
     7561 ttcgggcgag gcgtactcga tgtggcccat gtcgagataa atccggccgc cattaagcag
     7621 gaaaccgccg tttccgggag gctcttcgta gtcgcgatag tgcaggtcga tcgcgcccgc
     7681 gcgcattttg cggaagagat gattcttcac ccgcaccggc caggcgtcgc cattgggatg
     7741 cgcagcatcg ccgctcacca ggcagccgta ctcggtctcg atccctgcga tccgatccat
     7801 ccctccccat gatccgatag tggcgcgaga tggacaaggg acaaaacgga gcgcagcttt
     7861 cccgaatgag acccaaacca tttcctgcgc gcagtccggc cgagacggtt ggcggcctgg
     7921 tctactttgg tcgcatgctc gacaagatcc gcacgcgtgc caggggtgag ctcccggcgg
     7981 actacattcc caatctcggg aagggctttg atggccgctc tgtgcggttc ctcggtgtga
     8041 gctacgacga cgtcgtcgcg cgcgtgcggg agggcgggac ggacgaggaa atcctgcaat
     8101 gggctttcgc gaaaggtcgc aaaccctcag aggaggaagt ggaaatctgg aacgagttca
     8161 tgtgcaagtg cggctggaag gacaacatct cggaaacgct cgtgcgccgg aagaaagaga
     8221 gcgggctcac cgagcgcgac gatatcgaga cgatgtttca attcatcgat gccgacgaag
     8281 ggcgggctct gtagacgtcg cccggtgggc ggcgccagtg aaagcgtgct cggaagccgg
     8341 gccggcggcg tccgctttgc cccgcgttgc gcaaagcgaa gcggctattt ctatctcacc
     8401 agctcgccga tcttgttcgg tggtggcggc ggcttttgcc ggtaatcgat cacgacccaa
     8461 gtgatgatca ctaccacggc cacgagcaaa gccgacacga tcatccgccc tcgggtcggt
     8521 ctcgcggcct cggcctgcgc catggtcgtt attccgaaag ggtgtcgatc cgttccgcga
     8581 ggctgaagtc gaggtcggtc aacccaccct tgctatgggt ggagagaatc aggcgcacct
     8641 tgttgaagcg aatgtcaatg tcgggatgat gctcttcgtc ctcggccact tcggcgacgc
     8701 tgttcacgaa atcaatcgcc tcggaaaact cgtcgaactc gaacgtccgc tcgatgtgct
     8761 tcttctccag ctcccattcc gggatttttt tcaggcggtt cttcagctcg ctcgctttga
     8821 ttaactcggc catgacggca acatgagtaa catcccgttt tgcttttgca acggggaatc
     8881 caagcggttt ttcgcgtgct tttccggccg gtcaaagccc aaggatttgc cggaggtttt
     8941 tcgcggaatc gaaaacctcg acggcgcatt ctcgtgcctg ccgcgaaagc accgtccaat
     9001 cgcgcagcac ctggtgcgcg gccgccgccg cctcggccgc gccctcgacg aaaaacacgc
     9061 cgctcgtcgt cggaagatac tttccggctc cggtgttttc cgtgatcacc ggccgtccga
     9121 gggcaaggaa tgcggccgcg cgatcgctca tccagccagt ctgccaccgc acatcgacgc
     9181 ctttgatggc ggtaaactcg gcgacggccg aagcgagata gcgacggtag cgcgcaggtg
     9241 tgcgcgccac tcggtgcggg ccggccaact cccagccaag attcgttagg cggttcgcct
     9301 cgacggggtc cgcgccgatg ttcattgcca actcccattt tgcctccggc acgcgcgccg
     9361 gcaggtcgag gtaaggcgcg aaggcatgct gtttcgaaag atccgggaat tcgccatcca
     9421 cctcgatgct gccgtcccag taccattgcc cgatggtggt gaacttcggc cggcgcggtc
     9481 gtggttgggc ccggagcagt tccgtatccg ccaacggata gaaggtgcgc cagggaagag
     9541 aagttttcgg caggcggcaa tcggcgccgt gcacgttgag cccgatcgtc cagaactcgt
     9601 ggtgctggct ttggcccagt tcgagtttcg tcatccaata gaaaatttca cccggatcga
     9661 gatcgcagaa aatgcggcgt tcgaactcga acaaaaacgg cggatgaagt gaataggaca
     9721 ggttcagaag aacgttaggc gcagcgagcc gggcgcgcag ctcccgctgc gtcaggccga
     9781 tgcagcgaca tgccgccagg gtgtgtgcat cgctgaccgg atcctggtaa agcaggcaat
     9841 agcgtccagc gagcccatgg gcgcggagct gcctctggaa gttcgcgatg caagccgcgt
     9901 cgttggcgcg gtccgtcgtc gcggggagaa gctccagcca gaccgcatcc tgttgcagtc
     9961 gccgcaggcc gagcatccat tgcagcggga cggagaagtt gcccccgcct tcacgatatt
    10021 ttgcggcaaa tccgcagccg aggaaaaccg tcacgcgaag aagaaagagg ggaaaacctc
    10081 caacgtccaa ccccgaaagc tttcgagctt aaacctttct cgcctctttc ctgcgcgcag
    10141 ttgacctcgc cccgcgcctc tcttaattag cctccctcgc gcccgtagct caactggata
    10201 gagcatctga ctacggatca gaaggtttga agttcgaatc ttcacgggcg cgccaccctt
    10261 gcgtaaatga tgatgcgttt tgttttcatg gatcgcgggg acgccggatc tgatatgctg
    10321 cccagttgtg cgagcgggaa ggtgaagata gcggcgggaa gccgacgcaa gcgttcgtgg
    10381 tgccggtctt cgcggacggt cagaggaggc tctcgatcgg cagccagctc aggacgctga
    10441 ccttgatacg cttccagaga ctggtctcgg gctcagagtc gaagcggact tggttattgc
    10501 cctcgcgagt cacccactcg aggtgatttc ccacaagttc gacgcgatag gcgttgcggt
    10561 cgatatccct ttcggtcttc tccggcagac gctttgcaaa cgccggacag tcgaagagca
    10621 cacccatctc tgtgttcagc ttacttgagc gcgggtcgat attgtaggag ccaacaaaag
    10681 caatccggcg atcgaaggcg aacgtcttcg cgtgcaaact cgaactgccc gaaccggtcc
    10741 ctccgcgcga gctgtcgggc tcccgctcgt gaagggcgcc agcggtcggc ttcgtttcgt
    10801 agagttcgat tccggcttcg agcaacggct tgcggtagag cttgtatttc gcgtggactg
    10861 gcagcccgtc cgttgctgcc agtgaattgg tgatcacgac aacccgggcg ccgcgccggc
    10921 gaacgccagc cagaatttcg acgccctgct tccctggcac gaagtagggc gaaaccagga
    10981 acagctcgcg tcttgttcga tcgacgaccc cgcgcagcgt cggagccaga tgggtgtccg
    11041 cattcccgga cgaggtgagc accttgtcgg ggttatcgtt cacaatcgtg gcgcgtcccc
    11101 agtaaaacga aatcttccgg ctccgaagct gccgggcaaa tttgctctcg cgc
//
LOCUS       DATMNS010000120         6743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2677114, whole genome shotgun sequence.
ACCESSION   DATMNS010000120 DATMNS010000000
VERSION     DATMNS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2677114"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VNW28_04435"
     CDS             <1..369
                     /locus_tag="VNW28_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09203.1"
                     /translation="LISAFFAILFLQSGIDKVIDRRGNLEWLTGHFAKSPLAGMVPVL
                     LGVVTIMELSAGVLSAIGCVLILLRHDSTIAFYGVIFAGLALLALFFGQRMAKEYPGA
                     AALVPYFLVVIAGLIWLGQR"
     gene            413..1480
                     /locus_tag="VNW28_04440"
     CDS             413..1480
                     /locus_tag="VNW28_04440"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HXA09204.1"
                     /translation="MRVLLVALAAGHAAAVIAAAAREPVAPPPPGKLYQGFFFSAVSP
                     GSDDANEHNVTAGDVGQFEQAIGKQTSWIYFSDNWFESRTFPAATCGWIRSLGKVPYV
                     RLMLRSDLDQNHAEKIFSLANICAGRFDDDLKRWAEGASSFGGPILIEWGTEPNGDWF
                     AWNGKWNGRAEGPARYVQAYRHIVDLMRAAGANNLQWVWHVNWDDVPEVRWNRLENYF
                     PGETYCSWMAVSAYGPLTPRTAEVETFRAEIDQVYPRLTKFAPGKPIIVAEFGCDLHH
                     RKIAAAAWARDALHDLLGGRWPAIIGFCWWNESWENDDVAAHNSDMIILHDPALTAVF
                     RTGLEKQGDRIQETPLVPRQR"
     gene            1527..1934
                     /locus_tag="VNW28_04445"
     CDS             1527..1934
                     /locus_tag="VNW28_04445"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXA09205.1"
                     /translation="MVQISIDYEGKLHCNATHGPSGAPLATDAPLDNQGKGEAFSPTD
                     LVATALGTCIATTMAIVAERHGVELKGLTVQVAKEMASDPRRISRLTTEVHIPLPAAH
                     PQRELLEKTALGCPVHRSLSGEMERPTKFFWEG"
     gene            complement(1956..3002)
                     /locus_tag="VNW28_04450"
     CDS             complement(1956..3002)
                     /locus_tag="VNW28_04450"
                     /inference="COORDINATES: protein motif:HMM:NF014027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HXA09206.1"
                     /translation="MKTKPRLKSPEEINAALAAKKGPVSKFIARNYRHFNAAALLDAA
                     KGYEEHLAAGGKMLVTLAGAMSTAELGISLAEMIRRDKVHLIVCTGANLEEDIFNLVA
                     HDHYERVPHYRQLTPQDEQQLLSRHMNRVTDTCIPEMEAMRRIEKAVLEEWVAADRAG
                     EHYFPHEFMYQVLRSGKLKKSYQIDPKHSWMIAACEKNLAIIVPGWEDATLGNMYAAA
                     VIRGDVKNVHTVRTGIEYMTSLADYYTKNAKKLRNREGSIGFFQIGGGIAGDFPICVV
                     PMLHQDLGRTGVPLWGYFCQISDSTTSYGSYSGAVPNEKITWGKLAASTPKFVVESDA
                     TICAPLLFAWVLGQ"
     gene            complement(2999..3955)
                     /gene="rfaD"
                     /locus_tag="VNW28_04455"
     CDS             complement(2999..3955)
                     /gene="rfaD"
                     /locus_tag="VNW28_04455"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR02197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HXA09207.1"
                     /translation="MNTQPPSFLVTGGAGFIGSNLTLALQEKFPTARLTVIDDFRSGD
                     FKNLLGYRGDFVAADLATLDWMEQFGDATFDGIFHLASITDTTLHDQFPQVHDNVESF
                     RRLLRFSRRNRTRIVYASSAATYGAASGVNREADEAAPANVYAFSKVILDNLARRAAA
                     DDPQWKIVGLRYFNVYGPREAHKGVPASMILHLSRQMKAGKNPRIFKHGEQKRDFVYV
                     KDIVQGTLRALEARESGIYNLGSGQARSFNELIAILNKSLGTAFAPEYFDNPHAHYQN
                     HTEADLTNARKSLGYEPEFPLEKGVADYMRWLYPARPELPKS"
     regulatory      4017..4237
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4541..6415
                     /locus_tag="VNW28_04460"
     CDS             4541..6415
                     /locus_tag="VNW28_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXA09208.1"
                     /translation="MRYPERWFVAILCFLASLGVCPAQDAVAPPEAAEIVVSATRLPI
                     PEDEAPASVDVITSQDFEDKQTERVADALRQVAGLSVVQTGSPGQLTSVFTRGLRSEH
                     TQVLIDGVPINQGLAGLFDFADLTIDNLDRIEVVRGPQSTLYGPRALAGVIQIFTKEG
                     NETPETSASFEAGSYDTFRETLATSGKIGQVDYSFGGSRLDTDNARPNNQYRLWDFVG
                     NVGWSPDDQLRISSLFTYSRADTGNPNTIFDPKPLDNFLTERWLIAPHIDWKPSDEWE
                     HKLIVAYDEERQVNDPNQDGFVGPTRALFTRLTIDYQNNFRPAKWLTLTSGFFFSEVN
                     AGQARPFVTQAFGPQPTFVSDYTDERAIFLEASAMPLPGLNLVAGGRYDHFNQFGGIW
                     TYRVAGSYKIMPTDSIAHVSVATGFSPPTSQDKIFGNNFGLKPEKDLGWEIGVEQRLW
                     EERVKVAATYFHNDLSNLIGTNGLFDTLNLGAALTQGLEAEMELRPIADLVFSASYTY
                     LDAVNTSSADITQLPGARLPRRPRNEVYFSASYFWFRKLRTTAEAKFVNAREELNFGG
                     PNFDIEDYSFVNFAAEYVYNSHLTIFGRVDNLTNENYAEVFGFPALGRAAYGGIRISF
                     "
BASE COUNT         1287 a         1981 c         2072 g         1403 t
ORIGIN      
        1 ctcatctccg ctttcttcgc gatcctgttc ctccaatccg ggatcgacaa ggtcatcgac
       61 cgtcgcggca atctcgaatg gctgaccggt cactttgcga agagcccgct ggctggaatg
      121 gttccagtct tacttggcgt cgtcacgatc atggaactct ctgccggcgt tctcagcgcc
      181 atcggctgcg tcctgatcct gctccggcac gattccacaa ttgccttcta tggcgtgatc
      241 ttcgccggtc tcgctttgct ggctctcttc ttcggccagc gaatggccaa ggaatacccc
      301 ggcgcggccg cgctcgttcc ttattttctg gttgtgatcg ctggcctcat ttggctcggc
      361 caacgctgag gcgaaggttc ctgacgtggg caatcgtctt aggtggagga aaatgagggt
      421 cctcctcgtg gcacttgccg ccggccatgc tgccgcagtc attgcggccg ccgcgcgcga
      481 gccggttgct ccgccgccgc ccgggaaact ttaccagggc tttttcttca gtgccgtctc
      541 gcccgggagt gatgacgcga acgagcataa cgtcaccgcg ggagacgtcg ggcaattcga
      601 gcaggcgatc gggaaacaga cgagttggat ctatttttcc gacaactggt tcgagtcgcg
      661 gacgtttccc gcggcgactt gcggctggat ccgatcgtta ggcaaggttc cctacgttcg
      721 tttgatgctg cgttcggatc tcgaccagaa ccacgccgag aaaattttca gcctcgcgaa
      781 tatttgcgcc ggacgtttcg acgacgatct gaagcgctgg gccgaaggcg cgagttcatt
      841 cggcggcccg attctgatcg aatggggaac agaaccgaac ggggactggt ttgcctggaa
      901 cgggaaatgg aacggccggg cggaggggcc ggcgcgctac gtgcaggcct atcgccacat
      961 cgtggacctg atgcgcgcgg ccggcgcgaa caacctgcaa tgggtttggc acgtgaactg
     1021 ggacgacgtt ccggaagtgc gctggaaccg gttagaaaac tattttcccg gcgaaaccta
     1081 ctgcagctgg atggcggtca gcgcttatgg accgctcact ccgcgcaccg ccgaagtgga
     1141 gacctttcgc gcagagatcg atcaggttta tcctcgccta acgaaattcg cgccgggcaa
     1201 gccaatcatc gtcgcggaat tcggctgcga cctccatcat cgcaagatag cggcggcggc
     1261 ttgggcccgg gacgccctgc atgatctttt aggtgggcgc tggcccgcga tcatcggctt
     1321 ctgctggtgg aacgagagct gggagaacga cgatgtcgcc gcgcataact cggacatgat
     1381 cattctccac gacccggcgt taacggccgt ctttcgcacc gggctggaaa aacagggcga
     1441 tcgaattcag gagacgccgc tcgttccgcg ccaacgttag gcggatgcac ttttgctaat
     1501 tcgccgggag cgtccgatga tttcccatgg ttcaaatttc gatcgactac gaggggaaac
     1561 tccattgcaa cgcgacccac ggtccgtccg gcgcgccgct cgcgaccgac gcgccgctcg
     1621 ataaccaggg caaaggcgaa gcgttttccc cgacggacct cgtggcgacg gcccttggca
     1681 cctgcatcgc gacgaccatg gcgatcgtgg ccgagcggca cggagttgag ttaaaagggt
     1741 tgacggtgca ggtggcgaag gaaatggcga gcgacccgcg ccggatcagt cgcctaacga
     1801 ctgaggtgca cattcccctg ccggccgctc atcctcagcg cgagttgttg gaaaagaccg
     1861 cactcggttg tcccgtccat cgcagcctct ccggcgagat ggaacggccg acgaaatttt
     1921 tctgggaagg gtagtgggtt ggcaggtcgt atttcttact gcccgaggac ccaggcgaag
     1981 agcaatggcg cgcaaatcgt ggcatcgctt tcgactacga acttgggagt cgaggccgcg
     2041 agcttgcccc aggtgatttt ctcgttcggc acggcgccag agtagctgcc gtagctcgtc
     2101 gtcgaatcgc tgatttggca gaaatagccc cacagcggca ccccggtgcg cccaagatcc
     2161 tggtgcagca tcgggacgac gcagatcggg aaatcgccgg cgatcccgcc gccgatctgg
     2221 aaaaaaccga ttgagccctc gcggttgcgc agcttcttcg cgttcttcgt gtagtagtcc
     2281 gcgagcgagg tcatgtattc aatgcccgtg cgcacggtgt gcacgttctt cacatcgccg
     2341 cggattaccg ccgccgcata catgttgccg agcgtggcat cctcccagcc cggcacgatg
     2401 atcgccaggt tcttttcgca ggccgcgatc atccaggaat gcttcgggtc gatctggtaa
     2461 ctcttcttca gcttaccgct gcgaagaact tgatacatga actcatgcgg aaaatagtgc
     2521 tcgccggctc ggtcggccgc gacccactct tcgagcaccg ccttctcgat ccgcctcatc
     2581 gcctccatct ccgggatgca cgtgtcggtg acgcgattca tgtgccggga aagtaattgc
     2641 tgttcgtctt gtggagtgag ttgccggtaa tgcggcaccc gctcgtagtg gtcgtgcgcg
     2701 accagattga aaatatcctc ctcaaggttt gcgcccgtgc agacgatcag gtgcaccttg
     2761 tcgcgacgga tcatctcggc caatgagatg ccgagttcgg cggtcgacat cgcgcctgcg
     2821 agcgtcacca gcattttgcc gccggcggcg aggtgttctt catacccctt cgccgcatcg
     2881 agcaacgcgg cggcgttaaa gtggcgataa ttccgcgcga tgaatttcga aaccgggcct
     2941 ttcttggccg cgagcgcggc gttgatctct tccgggcttt ttaagcgggg tttggttttc
     3001 atgacttggg caattctggc cgggcgggat acagccagcg catgtaatcc gccacgcctt
     3061 tttccagcgg gaactccggt tcgtagccta acgacttccg cgcattggtt aggtcggcct
     3121 cggtgtggtt ctggtaatgg gcgtgcgggt tgtcgaaata ttccggcgcg aaagccgtgc
     3181 ctaacgactt gttcaggatc gcgatcaatt cgttgaagga ccgggcctgg cccgagccta
     3241 gattgtaaat cccgctctcc ctggcctcca gcgcgcgcag cgttccctgc acgatgtctt
     3301 tcacgtaaac gaaatcgcgc ttttgttcgc cgtgtttgaa aatgcgcgga ttcttgcccg
     3361 ccttcatctg gcgcgagaga tgcaggatca tgctcgccgg cacgcctttg tgcgcctcgc
     3421 gcgggccgta aacattgaag taacgcagcc cgacgatttt ccactgcgga tcgtccgccg
     3481 ccgcgcgccg ggcgaggttg tccaggatca ccttggaaaa agcgtagacg ttggccgggg
     3541 ccgcttcatc cgcttcgcgg ttgactccgc ttgccgcgcc gtaggttgcg gcggaggagg
     3601 cgtagacgat ccgggtgcgg ttccgacgcg agaaacgcag gaggcggcga aagctctcga
     3661 cgttgtcgtg cacctgtggg aactggtcat gcagcgtcgt gtcggtgatg gaggcgaggt
     3721 ggaaaatccc atcgaacgtc gcgtcgccga attgttccat ccaatcgagc gtggccaggt
     3781 ccgcggcgac gaagtcgccc cgatagccga gtaaattctt gaaatcgccg gagcgaaaat
     3841 catcgatcac ggtcaggcgc gccgtgggaa atttctcctg cagcgcgagg gtgagattcg
     3901 agccgataaa gcccgccccg ccggtgacga gaaaacttgg aggttgggtg ttcaaggagc
     3961 cgaatttgag acggtgacag ataaatcttg agcggcgaag cgcaagcggc tacttctgcc
     4021 gtcagttctt tgcgagtttg aaattgtgga tcggcgcgag aaatcccgtg tgaaccggga
     4081 gcagttgcgc tgctgtaact ggagacgact tcccatgagc caatcgttag gccgtgagtg
     4141 cggtgctggc ggtgaaggcg ggaagaaggt gtgcctcgaa tcttttcgag gccgggaacc
     4201 aggagccaga atatttgccc gctccacctc attcgtgtgg ttcgctgtag cggcggtctg
     4261 tgaccgccga tcttctacac gcgtccccgc gacaaaactt cttcgcgaaa aggaagaatg
     4321 agagacgaaa gcgccgcgca agtgacgagt ccgcctctca cagaatggag gttgtcagat
     4381 tatgtttgga ccggcgtttg gcgggacggt agaaagctcg ggtagcgcac gcattctgcg
     4441 tgctggtcgc ggtgttccac cgcgacgaac ttcccggagc gggatctatt cggaggaaaa
     4501 gttcgcgaca gggaaacacc gtcgcccgca cgcagaatgc gtgcgctacc cggagcgctg
     4561 gttcgtcgcg atcctctgtt ttttggcgtc gttaggcgtt tgccccgcgc aggacgcggt
     4621 cgcgccgccg gaggcggccg aaatcgtagt gagcgcgacc cgccttccga tcccggagga
     4681 cgaggcgccg gcgagcgtgg atgtcatcac gtcgcaggat ttcgaagaca agcagaccga
     4741 gcgcgtggcc gatgccttgc ggcaagtagc gggcctctcg gtcgtgcaaa ctggttcacc
     4801 gggacaactc acttccgtct ttacccgcgg tctgcgcagc gaacacacgc aggtgctgat
     4861 cgatggcgtg cccatcaacc aggggctggc cggtcttttc gatttcgccg acctgacgat
     4921 cgacaacctc gaccggatcg aggtcgtgcg cggaccgcag agcacgctct atgggccgcg
     4981 ggcgctcgcc ggcgtgattc agattttcac gaaagagggg aacgaaacgc cggaaaccag
     5041 cgccagcttc gaggccggca gctacgatac ctttcgcgaa accctcgcca cctcaggcaa
     5101 aatcgggcag gtcgactatt cctttggggg gagccggctc gacaccgaca acgcccggcc
     5161 taacaatcaa tatcggctct gggattttgt cggcaacgtc ggctggtcgc ctgacgacca
     5221 gttacggatc agcagtcttt tcacctactc gcgggccgac accggcaatc cgaacacgat
     5281 tttcgacccg aagccgctcg ataattttct caccgaacgc tggctgatcg cgccgcacat
     5341 cgattggaag ccgtccgacg agtgggagca caaattgatc gtcgcctacg acgaagagcg
     5401 gcaggtgaac gaccccaacc aggacggttt cgtcggtccg acgcgcgccc tcttcacccg
     5461 cttaacgatc gattatcaaa acaactttcg tccggccaag tggctgactc ttacctccgg
     5521 cttcttcttc agcgaagtca atgccggcca ggcacggcct ttcgtgacgc aggctttcgg
     5581 gccgcagcca accttcgtca gtgactacac ggatgagcgc gcgatttttc tggaagcgag
     5641 cgccatgccg ctccccggcc tgaacctcgt cgccggcggc cgctacgatc acttcaatca
     5701 gttcggcggg atctggacct accgcgtggc cggcagctac aagatcatgc cgaccgattc
     5761 gatcgcgcac gtcagcgttg cgaccggatt cagtccgccg accagccagg acaagatttt
     5821 cgggaacaat tttggactta agccggagaa ggatcttggc tgggagatcg gggtcgagca
     5881 acgcctctgg gaggaacgcg tgaaagtcgc cgctacgtat ttccataatg acctttcaaa
     5941 tttgatcggt actaacggac tcttcgatac gctcaacctt ggggcggcgc taacccaggg
     6001 tctggaggcg gaaatggagc tgcggccaat cgccgacctg gtcttctcgg cgagctatac
     6061 ctacctcgat gcggtcaaca cctcctcggc cgacatcacg caattgccgg gcgcgcgtct
     6121 gccacggcga ccgcgcaacg aagtttactt ctccgcctca tatttttggt tcaggaaatt
     6181 gcggacgaca gcggaggcga aattcgttaa tgcgcgggag gaactgaact ttggcgggcc
     6241 aaatttcgac atcgaagatt attcgtttgt gaatttcgcg gccgaatatg tttacaattc
     6301 gcacctcacg atattcggcc gggtagataa cctaacgaac gagaattacg cggaggtatt
     6361 tggttttcca gccctgggga gggcggccta cggaggaatt cggatttctt tttagtcatc
     6421 ccgggcggag tcgagggatc ccggggcgtt cgctcgaacg gagcgaaaac agaatcaacc
     6481 tggaggcggg caagtgagct tctcggtcgc gccgggcaag cttctcggta gcgcgacggg
     6541 attcctcgac tccactcggg atgacaacct ggtgtgcgcg ccgcttgaaa gcgaatcgcg
     6601 ccactgttaa gcgctaggag tggatccggt tttcgccgcc ctgctcgggc tcatcgttgg
     6661 cgtggtcgcg gtggggggag ttttcctgct gattcgccat cgtcgctcgg acgccgcgca
     6721 attgatcgaa cgcttcgaag ccc
//
LOCUS       DATMNS010000121         5038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_526476, whole genome shotgun sequence.
ACCESSION   DATMNS010000121 DATMNS010000000
VERSION     DATMNS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5038
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526476"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..954
                     /locus_tag="VNW28_04465"
     CDS             283..954
                     /locus_tag="VNW28_04465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal response regulator transcription
                     factor"
                     /protein_id="HXA09209.1"
                     /translation="MHILIVEDEQKIAAFIRRGLMENGFVVDVAEDGEQGLEYALARK
                     VDLIILDVALPKRDGWSVMTELRRAEIHTPVLFLTARDSVPDRVKGFDLGADDYLIKP
                     FHFSELLARVRSLMRRSPERREETITVGDLEIDPRRHRVARSGTAINLTPKEFALLLF
                     LASSAGDIVSRTEIVEQVWDINFDTGTNVVDVMMRRLRAKVDDPFEKKMIQTIRGVGY
                     VLQTD"
     gene            935..2368
                     /locus_tag="VNW28_04470"
     CDS             935..2368
                     /locus_tag="VNW28_04470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal sensor histidine kinase"
                     /protein_id="HXA09210.1"
                     /translation="MSSKPTDSIPVPKTLFGRRSWSIAFRLVLLFTIAAAVLLLATMA
                     ASLFTVMKHVEHDNDRYLTDKLAALRADIASDAGPQSLANELKIIRAADKAYAVRVLN
                     PAGKVVAESPSMERLLPMEVFPRSLWLPGQRPEPEIYYARNGKTFALVTSFADVGSQR
                     LTVQLAQDRTHDEQFASRYGALLTGVLGCAVLTCAGVALLVTRRGLRPLKQLAESVER
                     IGATHLDERVLVERWPDELQPLALGFNKMLGRLEDSFTRLSHFSADLAHELRTPIAIL
                     RGEAEGVLTKPRTPEQYREVIESGLEELQRLSGMIDNLLFLARAETIGPLNRSYIDGR
                     AAIEKIREFYEAVSQDQGVEIVCRGEGKVYAEPVLFRRALINLITNALRFTPSGGSVT
                     VSLVHQKGESNVSVADTGCGIPSEHVPNLFDRFFRGDASRSAQGTGLGLSIVKSIMQI
                     HNGDVSVQSDLGKGTIVTLRFPDLEPA"
     gene            2507..3934
                     /locus_tag="VNW28_04475"
     CDS             2507..3934
                     /locus_tag="VNW28_04475"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HXA09211.1"
                     /translation="MISKALFVSLLLVATQSLLGYALEGESWTLNRTVVMQLSLGGPR
                     SLSDGFTSFNASAQDALNLWNQHLTHLQFTTVLASPVTPATGDDENSAFFSNTVFGDQ
                     FGTNVLAVTAYNFRGTTMEESDTVFNSAYTWDSYNGPLNVSVPDFHRVALHEFGHNLG
                     LNHPDQAGQTVAAIMNSRIGNIDTLQPDDIAGVQFLYDTGPAYQSSVPAPVLANISTR
                     AFIGAGDNVLIGGFIVQGTQPATVILRAIGFSLSAIGFTDAIMDPTITVYDSNQQQVA
                     MNDDWAFTGADAETIGSYHLDPPNSRESALYLTLQPGAYTAVVQSFLDAENPPVPGIG
                     LFELYDLHTTGGRAGNISTRGQVLGGDNVLIGGLIIGGTEIKTIIARAIGPSLGAAGV
                     ANPLADPTLELHDSNGNLLQSNDDWQAGPDAQAITSDGLAPTNPKESALLATLNPGAY
                     TAIVRGVNGATGIGLVEVYDLSPVP"
     gene            complement(3910..4767)
                     /gene="proC"
                     /locus_tag="VNW28_04480"
     CDS             complement(3910..4767)
                     /gene="proC"
                     /locus_tag="VNW28_04480"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HXA09212.1"
                     /translation="MSLQTNDQRYRLGFIGGGKLAGSVIRGLVRSKFCAPEEILVSEP
                     YEAARASLRDEVGISVTAENAEVAEQTEVILIGVKPGVVLHVIAEMAAALDGKLVISL
                     AAGVGVAAMEGKGRARFMRAMTNTPAAVCQAATAVARGSRSTAGDLARAREIFAAVGT
                     VTEVEEGQIDAVTALAGSGPAFVYTVIEALAAGGTNCGLPAEISLGLATQTVRGAAQL
                     ALESKLSPEELRRMVITPGGTTAAGLARMEERQTAAGLSAAVEAAAARAKELLPPPDE
                     NNYGTGLRS"
     gene            4790..>5038
                     /gene="nadA"
                     /locus_tag="VNW28_04485"
     CDS             4790..>5038
                     /gene="nadA"
                     /locus_tag="VNW28_04485"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HXA09213.1"
                     /translation="MVSLGVNFGLDLVAEIKELKNERRAVILAHNYQVPEIQDVADFV
                     GDSLGLSFQAAKTDAEVILFCGVHFMAETAKILNPAKRV"
BASE COUNT          980 a         1604 c         1434 g         1020 t
ORIGIN      
        1 tatttgctat cgaccgactt taccaggcag caagaccgaa ttcgaattct gtcaagatcg
       61 taattctatg gtcatgtcca tcgctcggtc gtggtttccc gttcccgctc tgccggctgt
      121 gcgaaatggc agacctttcc agtccgccaa tgaaagatgc tggtcaaagc tggcaaagca
      181 gaccatggtt tgtcggaaca acaccgatca gcccgcgaat ggttcgtagc ctggtcaacc
      241 acttagcaaa gacgaagcca ctgtttggag tcactaacgc ctatgcacat tctgattgtt
      301 gaagatgaac agaaaatcgc cgcctttatc cggcgaggtc tgatggagaa cggcttcgtc
      361 gtagacgtgg ccgaagatgg cgaacaaggt ttagaatacg cgctcgcccg aaaagtggac
      421 cttatcattc tcgatgtcgc gctgcccaag cgcgacggct ggtcagtgat gaccgagctt
      481 cgacgcgcgg agattcacac cccggtgctc tttctaacgg cgcgcgacag tgtgcctgac
      541 cgggtcaaag gatttgacct cggagccgat gactatctta tcaagccctt ccatttctcg
      601 gaacttctcg cgcgcgtccg ttcgctcatg cgacggtcgc ccgaacgccg ggaggagacg
      661 atcacggtgg gggatctgga gatagatcct cggcggcaca gagtagcgcg gtcgggaacg
      721 gccattaacc ttacgcccaa ggagttcgcg ctactcttat ttctcgcttc ctccgccggt
      781 gacatcgtct cgcggaccga gatcgtcgaa caagtctggg acattaactt tgataccggg
      841 actaacgttg tcgacgtcat gatgcgccgt ttgcgggcca aggtggatga ccccttcgag
      901 aagaagatga tccagaccat tcggggcgtt ggttatgtcc tccaaaccga ctgattccat
      961 ccccgtcccg aagaccctgt ttggtcgccg ctcctggtcg atcgcgtttc ggctggttct
     1021 gctctttacg attgcggccg ccgtgctgct gctcgcgacg atggctgctt ccttgtttac
     1081 cgtgatgaaa cacgtcgagc atgacaacga ccgctacctg accgacaagc tggccgcact
     1141 ccgcgccgac atcgcctcgg acgccggccc ccaatctctc gccaacgaac taaagatcat
     1201 ccgcgccgcc gacaaagcct atgcggtccg agtactgaac ccggctggga aggtcgtggc
     1261 ggaaagccca agcatggagc ggctcctgcc gatggaggtc tttcccaggt cgctctggtt
     1321 gcccggacag cgccccgagc ctgagattta ttacgctcgc aatggcaaga cttttgcgct
     1381 cgtgacctcg ttcgccgatg tcggcagcca gcgcctcacg gtgcaactcg cgcaagatcg
     1441 aacgcacgac gaacaatttg ccagccgcta tggcgcgctc ctcaccggcg tgctcggttg
     1501 cgcagtgctg acctgcgcgg gagtcgcatt gctggtcacc cgccggggac tccgcccgct
     1561 gaagcagctg gcggagtcgg tcgaacgaat cggcgcgacg catctggatg agcgggtcct
     1621 ggtcgagcga tggccggatg aactgcaacc gctggcgctc ggatttaaca agatgctcgg
     1681 ccggctcgaa gactccttca cccggttatc gcatttttcc gccgatctcg cccatgaatt
     1741 gcgcacgccc atcgccatcc tccgcggcga agcggaagga gtgttaacca agccgcgcac
     1801 cccagaacaa taccgtgagg tgatcgagtc cggcctggag gagctgcaac gcctctccgg
     1861 aatgatcgat aacctgcttt ttctggcgcg cgcagagacg attggtccac tgaatcgcag
     1921 ttatatcgac gggcgcgcgg cgatcgagaa gatccgggaa ttctacgaag cggtctcgca
     1981 ggatcaggga gtcgagatag tttgtcgcgg cgaagggaaa gtgtatgccg agcctgtcct
     2041 gttccggcgg gcgctgatca acctgatcac gaacgcgctg cgtttcactc cctcgggcgg
     2101 cagtgtcact gtctcactcg tccatcagaa gggcgagagt aacgtgtcag tcgccgacac
     2161 cggttgtggc ataccttccg agcatgtccc aaatttgttt gaccgcttct ttcggggcga
     2221 tgcttcgcgc agtgcccaag gcacgggact tggcctgtcg attgtgaaat cgatcatgca
     2281 aattcacaac ggcgacgttt cggtgcaaag cgacctcggc aaagggacga tcgtgaccct
     2341 gaggtttcca gaccttgaac ctgcatagtc aaaggtgttt gttggcacgg aagttatcgg
     2401 gaaggcagca aacgcaaggg taagcatgaa acgagcatcc ttccggccga caggaattgg
     2461 cggttccgat tttcgcgtgc aaagacattc tgctcttcgc ccagtgatga tatcgaaagc
     2521 actttttgtt tccctcctgc tcgttgctac ccagtcgctt ctcggctacg cgctggaagg
     2581 tgaatcctgg acgctgaatc gcacggtcgt gatgcagtta tcactcggcg ggccgaggtc
     2641 cctgagtgat ggattcactt cctttaacgc atcggcccag gacgcgctca acctctggaa
     2701 ccagcatctc actcatttgc aattcacgac cgtcctcgcc tcgccggtca ctccggcaac
     2761 cggggacgac gaaaactcgg ctttcttctc caataccgtg tttggcgacc agttcgggac
     2821 taatgtgctc gcggtgaccg cctataactt tcgcggcacg acgatggaag aaagcgacac
     2881 cgtctttaac tccgcctaca cgtgggattc ctacaacggc ccgctcaacg tcagtgtccc
     2941 ggattttcac cgcgtggcgc tccatgaatt cgggcataac ctcgggctga atcaccccga
     3001 ccaggccggt caaaccgtcg ctgccatcat gaactccagg atcggcaata tcgataccct
     3061 tcagccggac gatatcgccg gggtgcagtt cctctacgac accggccccg cctatcaatc
     3121 ttccgtgccc gccccggtgc tagctaatat ctccactcgc gccttcatcg gcgcgggcga
     3181 caacgtgttg atcggtgggt tcatcgtgca aggaacgcaa ccggccaccg tcatcctgcg
     3241 cgcgatcggt ttctcgctca gtgcgatcgg cttcacggac gcgatcatgg atccgacgat
     3301 tacggtttat gattcgaacc agcaacaagt cgcgatgaac gacgattggg ctttcactgg
     3361 ggccgacgcc gagaccatcg ggagctacca tctcgatccg cccaacagcc gcgaatccgc
     3421 cctttatctc accttgcaac cgggcgccta taccgcggtc gtgcagagct tcctcgatgc
     3481 tgagaatccc ccggtgccgg gcatcgggtt atttgaactc tacgatcttc acaccaccgg
     3541 cggccgcgcg ggcaacatct cgacccgcgg tcaggtcctg ggcggcgaca acgtcctgat
     3601 cggcggattg attatcggtg gaacggaaat caagacgatc atcgcgcgcg cgatcgggcc
     3661 ttcgttaggc gcggccggcg tggccaaccc gctggccgat ccgacgctcg agttgcacga
     3721 cagcaacggc aatctcctgc agtcgaatga cgactggcaa gccgggccgg atgcccaggc
     3781 gatcacttcc gatggcctgg cgccgacgaa tccgaaggaa tcggcgctcc tcgcgacgct
     3841 taatcccggc gcctataccg cgatcgtccg tggcgtgaat ggcgcgaccg gcatcgggct
     3901 ggtggaagtc tacgacctga gcccagtccc gtaattgttt tcgtcagggg gcggcagcaa
     3961 ttccttggcc cgcgccgcgg cggcttcgac cgcggccgac aaccccgcgg ccgtttgccg
     4021 ctcctccatg cgggccaggc cggcggccgt cgttccgccg ggtgtgatca ccatccgccg
     4081 gagttcctcc ggcgacagct tgctttcgag cgcgagttgc gcggcgccgc ggaccgtttg
     4141 cgtggccagg cccaacgaaa tctccgcagg caatccgcaa ttcgttccgc cggccgccag
     4201 cgcttcgatc accgtgtaga cgaacgccgg cccgctcccg gcgagcgcag tcacggcgtc
     4261 gatctgcccc tcctcgactt ccgtcaccgt cccgaccgcc gcaaaaattt cgcgcgcccg
     4321 agcgagatcc cccgccgtcg agcgcgaccc gcgcgccacc gccgtcgccg cctggcagac
     4381 cgccgccggc gtgttcgtca tcgcccgcat gaaacgcgcc ctccccttcc cttccatcgc
     4441 agcgacaccg accccggcgg cgagcgaaat gaccagcttg ccgtcgagcg cagccgccat
     4501 ttcagcaatc acatggagca ccactcccgg cttcactccg atcagaatca cttcggtttg
     4561 ctcagcgacc tctgcgttct cagcggttac cgagattccc acctcatcgc ggagggacgc
     4621 gcgtgctgcc tcgtaaggct cgctcaccag aatttcttcc ggcgcgcaga atttcgaccg
     4681 caccaggccg cggatgaccg agccggccag tttgccgccg ccgataaaac cgagccgata
     4741 ccgctggtcg ttggtttgta aactcattgg gatacgcgga tattagccga tggtaagcct
     4801 cggtgtaaat ttcggtctcg accttgtcgc ggaaatcaag gagctgaaaa acgaacgccg
     4861 ggccgtcatt ctcgcgcaca attaccaggt gccggaaatc caggatgtcg cggatttcgt
     4921 cggcgattcg ttaggcctga gcttccaggc cgcgaaaacc gatgccgaag tcattctctt
     4981 ctgcggcgtc cacttcatgg ccgagaccgc gaagattctg aacccggcca agagagtc
//
LOCUS       DATMNS010000122         9489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4828135, whole genome shotgun sequence.
ACCESSION   DATMNS010000122 DATMNS010000000
VERSION     DATMNS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9489
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4828135"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VNW28_04490"
     CDS             complement(<1..215)
                     /locus_tag="VNW28_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09214.1"
                     /translation="MLLALSLSSARADWRLGAETGAFYESNLSNSDRASDIQDDWAWR
                     SKVSVGNGYQISRDLRLTLGADLRGQI"
     gene            complement(325..1194)
                     /locus_tag="VNW28_04495"
     CDS             complement(325..1194)
                     /locus_tag="VNW28_04495"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HXA09215.1"
                     /translation="MPPKTIGLIAHTGKAGAADLVRAIVQEFGRVSLPVKLEAETASL
                     AGQKSELSMQDLGRETDLLVVLGGDGTILNVVGKLGNDIKPIFGINIGSLGFLTCVAS
                     TAYREAVASIAAGEIEFSERALLEVHLDPQGPETHQVLRCLNDAVFSRGEISRLIRLA
                     VRVNGEVLTEFNADGLILATPTGSTAYSLSAGGPILAPDSGALVITPICPHTLSNRSI
                     IVDDSAVVEVEATERDHPVFLTVDGREPVRLEAGSAVTVRKSPQTLPLAVMPGVSFFS
                     VVRQKLRWRGSSI"
     gene            complement(1170..1910)
                     /locus_tag="VNW28_04500"
     CDS             complement(1170..1910)
                     /locus_tag="VNW28_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HXA09216.1"
                     /translation="MARGIRLDQALVQRGLFPSRHQAQTAIMAGEVQIDGRTAAKASD
                     FVQDQNEISVAERRRYVGRGGIKLEGALAHFGIDCEGKTALDIGASTGGFTDCLLQHG
                     ARKVFAVDVGHGQLAWKIRDDPRVTTLEKTNARHLSREEIPDPIELCVIDVSFISLTL
                     ILPNAFELLTPDGVILALIKPQFELDREDVGRGGIVRSAALHEKAQQKVAAFAETIGA
                     RVGGLFPSSITGTDGNQEFFLCLQKPSA"
     gene            complement(1915..3231)
                     /gene="bioA"
                     /locus_tag="VNW28_04505"
     CDS             complement(1915..3231)
                     /gene="bioA"
                     /locus_tag="VNW28_04505"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HXA09217.1"
                     /translation="MTRRAMDSWDTARLVADDKRYVWHPFTAMGDWCAPEHEPIVLVG
                     GEGAIVRDSRGREYIDGNSSIWTNIHGHNHPRINAAIREQLERVAHTSFLGTTNPPAI
                     DLAREIVQLFPPEKFGRVFYSDDGSTGMEAALRIVSQYWRLKESKRRVFISFRDAYHG
                     DTAGAASLGAAAMFGSAFGDWDLPVQRIGSVAELAAISNTDEIAAVVIEPIIQGAAGM
                     KIWPSGTLKRLREWCDSVGTLLLADEVMTGFGRTGRMFACEHEETHPDVYVFAKGLTG
                     GYSPLALTLVSHEISAPFCASGSDATLFYGHSYTGNALGCAAALASLEVFRTEGTIAS
                     LPPKMAEVREGLAAIGELRGVTGARTCGMIGAFDLPDNRLATRVCLEARERGLLTRPI
                     RNTVILMPPLIISMSQLKTALNALKQSLRTVITQKSHQFEPAPVTS"
     gene            complement(3209..3469)
                     /locus_tag="VNW28_04510"
     CDS             complement(3209..3469)
                     /locus_tag="VNW28_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09218.1"
                     /translation="MRSQHIWTSRDEDGRKREVRVTKFGGHWKFQAKFHDEERWTYYD
                     LPPLGDVRELRDIIFRKYQRRRASAEDFAAVDKMLRDQTRDG"
     gene            3496..4404
                     /locus_tag="VNW28_04515"
     CDS             3496..4404
                     /locus_tag="VNW28_04515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXA09219.1"
                     /translation="MKDAARLLLYLASVVVLGALAAPPLFWFGQYLIAQRILPGLAGF
                     DFETYFHRALLIAALVLLWPLLRAVRVRGWRDLGLEKNAHAGADVTAGFVIAAVPLLC
                     CGAVLVALHIYSPRQAFLWHKMPDVLVAASVVPVLEELLFRGFILGLLLRSFSRLGAL
                     ILTSTLFSIIHFLKAPELTTPNDAVAWSSGFVSIGHSFWQFGDPVLLAAGFLTLFAIG
                     CILADARLQTRSLWLSIGLHAGWIFANGAFSRATRRAAIALPWVGKDLLVGIVPLIIA
                     LASWALMRGWARYADPRAPQSVPRAR"
     gene            4361..5089
                     /locus_tag="VNW28_04520"
     CDS             4361..5089
                     /locus_tag="VNW28_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HXA09220.1"
                     /translation="MLIRALPNPFRGLVSLFYPAFCAVCSGAVEPREDLCAVCAGQAT
                     RIVPPFCAKCSQPFDGAITGTFTCANCQDRVLHFEAAVSAYRSRGVVRKVMLDFKYGR
                     QIHLRHLLGRWLSETLADPRLAGRHFDLIVPVPLHPAKKRERGFNQAELLAAVLRRRS
                     GVRVENVLQRTRYTLTQTQFDRSERMENLRGAFRLRRGSNVQDLRMLLVDDVLTTGST
                     LSECASVLKKAGAHSVHAATAARG"
     gene            5093..5938
                     /gene="accD"
                     /locus_tag="VNW28_04525"
     CDS             5093..5938
                     /gene="accD"
                     /locus_tag="VNW28_04525"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HXA09221.1"
                     /translation="MAIFKKPALKTESRKKREMPPGLWQKCDGCGEVVHEIELVENDR
                     VCPRCDYHFTQTAKERIAKLCDPDSFVELDARLTSIDTLKFQGMASYKDRLRKYQDKT
                     GLVDAVLSGHALIEGYKVAIAVMDFSFLAATMGSVVGERITRVIEYGTAHRAAVIIVA
                     ASGGARMYEGMLSLMQMAKTSGALALHGERRLPFISVLTNPTTAGVMASFASLGDVII
                     AEPKAMVGFAGPRVIKETTHQDLPERFQTAEFLEEHGLIDLIVHRKMLRAQLAQLLAY
                     FSSTR"
     gene            5956..7212
                     /locus_tag="VNW28_04530"
     CDS             5956..7212
                     /locus_tag="VNW28_04530"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HXA09222.1"
                     /translation="MPRSAGAGLRPRTPYEEALAWLFGTQRFGIKLGLENSRRLFAAL
                     GVLSPNDRIIHVAGTNGKGSVCALLDSICRAAGYRSALFTSPHLVTFRERIQLNGELI
                     SEEEVARGLTKIRELVAGWDPHPTFFEITTGLALDYFRRREAEIIVLETGMGGRLDAT
                     NATQPVVSVLTPIDYDHQKWLGETLTEIAGEKAGIIKAGIPVVSARQPEEAAAVIRAR
                     AAECGTPLAFVRQPFDRFPIALHGRHQKENAALAISALHTAQIPIDDAALERGLATVV
                     WPARFQQWNARTIIDGAHNPAGARVLAETWRQIFGDRRATILLAVLLDKDVAGICSAL
                     EPIARRALLPAIRSERALPPEELRLTICDRLPGLPVAVAPSLAAAWEEARRDAAPILI
                     CGSLHFAGEALAFLSGEPAAFEECLQ"
     gene            7404..8486
                     /locus_tag="VNW28_04535"
     CDS             7404..8486
                     /locus_tag="VNW28_04535"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HXA09223.1"
                     /translation="MTATANIVHLSPPTRARTCDQRSPAVSARRFLRPLLGCGLVVST
                     LCVLGVLVWQGITAHGAPDPTQPQTSPAMAFLDIGVLVFREGLECILVLAALTASMTG
                     DKAIHRRPVALGAGLGFVATGVTWLIAIGIMKQLIDSVPALDLQAATGLLAVVVLVVI
                     MNWFFHKIYWGGWIRAHNRAKKNLLASATDNHGSQRKLLWGLVLLGFTSLYREGFEVV
                     LFLQSYRLKFGNSVVLEGALVGLILSGMVAVLTFGLQRRLPYRQMLITTGVLLGGVLL
                     VMVGEQAQEMQLAHWISTTPIPALEPIVRPWMGMWFAVFPTVETLAAQAIAAIVVIGS
                     YYAARRMGRAAAAAAVALPRKKLLGG"
     gene            8739..9290
                     /locus_tag="VNW28_04540"
     CDS             8739..9290
                     /locus_tag="VNW28_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXA09224.1"
                     /translation="MRSRYRILEKQHAHFVTCTIVVWLPIFTTAARCDILVEALTYCR
                     AHKGLEIYAWVILDNHFHAILGAPDLARVMADLKRHTARRLVEQLETEGCVWLLHQLQ
                     HYRLAHKNESVHQVWQEGSHPKAMMSDAMMEQKLEYLHHNPVKRGLVSPRPGIGVIPR
                     RTSGSRARSRSCGWIPGVKAVPR"
     gene            9304..9456
                     /locus_tag="VNW28_04545"
     CDS             9304..9456
                     /locus_tag="VNW28_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09225.1"
                     /translation="MVRSQVQLGNETLDNPVKRGLVSSPEHRRYSSAHEWLPGALPRL
                     RVDAWG"
BASE COUNT         1677 a         3085 c         2874 g         1853 t
ORIGIN      
        1 caaatctgac ctcgcaaatc agcgcccaga gttagtcgaa gatcgcggga gatttggtag
       61 ccgttgccga cgctcacctt gcttcgccac gcccaatcgt cctggatatc cgaggcacgg
      121 tccgaattgg aaagattgct ctcgtaaaac gccccggtct ccgcgccgag ccgccaatca
      181 gcgcgcgcgc tgctcaagct cagtgccagc aacacaccca cgaatgctga tctcgccgtt
      241 ttcatccgcg ccccaggtcg ggaattccct cagcccgatt agaagagttt cgttccggcg
      301 ccgttaagtc aatcgcccac tgctttagat cgagctcccg cgccatctca gcttctgccg
      361 gacgacgctg aagaaggaaa cgcccggcat gacggccaac gggagagttt gcggcgactt
      421 gcgcacggtc accgcggagc cggcttcgag ccgcaccggc tcgcgcccgt cgaccgtgag
      481 gaagaccgga tgatcgcgct cggtcgcttc gacctccacg accgctgaat cgtcgacgat
      541 aatggagcgg ttggagagcg tgtgcgggca gatcggggtg ataaccagcg ccccggaatc
      601 gggcgcgagg atcggcccgc cggcgctcag ggaatatgcg gtcgaaccgg tgggcgtggc
      661 gaggatgagg ccatcggcat tgaactccgt gagcacctcg ccgttgaccc gcaccgcgag
      721 acggataagg cgtgagattt cgccgcgact aaagacggcg tcgttgagac agcgcaggac
      781 ctgatgtgtc tccgggcctt gcggatcgag gtgcacttcg agcagggcgc gttcgctgaa
      841 ttcgatttcc ccggcggcaa tgctggccac ggcttcgcgg taggccgtgg aagccacaca
      901 agtaagaaag cctaacgagc caatgttgat tccaaagatc ggcttgatgt cgttgccgag
      961 tttcccgaca acgttcagga tggtcccgtc gccgccaagg accacgagca ggtccgtctc
     1021 ccggcccagg tcctgcatgg aaagctcgga tttctgcccg gcaaggctgg ccgtctccgc
     1081 ttcaagcttc accggcaacg agacgcggcc aaattcctgc acgatcgcgc gcacgaggtc
     1141 ggcggccccg gctttcccgg tatgcgcgat caggccgatg gtttttggag gcatagaaag
     1201 aattcctggt tgccgtcagt gccggtaatg ctggagggaa acaggccccc cacccgcgcg
     1261 ccgatggttt cagcgaaggc ggcgaccttc tgttgcgcct tctcgtggag cgcggcggag
     1321 cgcacgatgc caccccggcc gacgtcctcg cggtcgagct cgaattgcgg tttgatcaag
     1381 gcgaggatca caccgtctgg agttagcaat tcaaaggcat tgggcaagat caaagtcagc
     1441 gagataaaag aaacatcgat cacacaaagc tcgattggat cggggatctc ttcccgggaa
     1501 agatgccgcg cattcgtttt ttccaaagta gtgacccggg gatcgtcgcg gattttccag
     1561 gcgagctggc cgtggcccac atcgaccgcg aagaccttgc gggcgccgtg ctggagcaga
     1621 caatccgtga aaccgccggt cgaggccccg atgtcgagtg cggtttttcc ttcgcaatcg
     1681 atcccgaaat gagcgagcgc accttccagc ttgatcccac cgcgacccac gtagcgccgg
     1741 cgctccgcga cggagatttc gttctggtcc tggacgaaat ccgaggcttt cgccgcagtc
     1801 cggccgtcaa tttgcacttc gccggccatg atcgcggtct gcgcctggtg gcggctcggg
     1861 aaaagtcccc gctggacgag cgcctgatcg agtcgaatcc ctcttgccat gcgtttacga
     1921 ggtcaccgga gcaggctcga attgatgtga tttctgcgta ataacagtcc gaagcgattg
     1981 ttttagagca tttaacgcag tcttcaattg cgacatcgag atgatcagag ggggcatcag
     2041 gatgacggtg ttgcggatgg gtcgtgtaag cagtccccgc tcccgcgcct caaggcagac
     2101 tcgcgtcgca agccggttgt cagggagatc aaaggcgccg atcattccgc aggtgcgagc
     2161 gcccgtgacg ccgcgaagtt ccccgattgc agccaagcct tcccgaactt ccgccatctt
     2221 cggcggcagc gatgcgatcg tcccttccgt tcgaaaaact tcgagactcg cgagagcggc
     2281 cgcgcagccg agcgcatttc cggtatagct gtgcccgtag aacagggtcg catcagagcc
     2341 agaggcacaa aacggcgcgg aaatttcatg ggaaacgagc gtcagcgcca agggcgagta
     2401 acccccggtc aatcccttgg cgaaaacata gacatcggga tgcgtttcct catgctcgca
     2461 ggcgaacatg cgacccgtgc gaccgaaacc ggtcatcact tcgtccgcaa gcaggagcgt
     2521 cccgaccgag tcgcaccatt cgcgcagccg cttgagcgtg cctgaaggcc aaattttcat
     2581 tcccgctgcg ccctgaatga tcggctcgat cacgacggcg gcgatctcgt ccgtgttcga
     2641 aatcgccgcc agctccgcga ccgagccgat tctctggacc ggaaggtccc aatcgccaaa
     2701 cgcgctcccg aacatggcgg ccgccccgag gctcgccgcg cccgccgtgt cgccatgata
     2761 tgcatcgcga aacgagatga agacccgccg ctttgattct ttcagccgcc aatactggct
     2821 aacgatgcgc agcgcggcct ccatcccggt cgagccgtca tcggaataaa aaacgcgccc
     2881 gaacttttcc ggtggaaaca attgcacgat ctctcgcgcc aggtcgatcg ccggaggatt
     2941 ggtcgtcccg agaaacgacg tgtgcgcgac gcgctcgagc tgttcccgga tcgcggcgtt
     3001 gatccgcgga tgattgtgac catgaatgtt cgtccagatg gaagaattcc cgtcgatata
     3061 ctcgcggccc cggctgtcgc gcacgatcgc cccctcaccg ccgacgagaa cgatcggctc
     3121 atgttccgga gcgcaccaat cccccatcgc ggtaaacgga tgccagacgt agcgcttgtc
     3181 gtcggcgacc aaccgcgcgg tgtcccagct atccatcgcg cgtctggtca cggagcatct
     3241 tgtcgacggc cgcgaaatct tccgccgagg cacggcggcg ttgatatttt cggaaaataa
     3301 tgtcgcgcaa ctcgcgcacg tcaccgagtg gcggcaggtc gtagtaggtc catcgttctt
     3361 cgtcgtggaa tttcgcctgg aacttccagt ggccgccgaa tttcgtcacg cgcacctcgc
     3421 gtttgcgtcc gtcttcatcc cgcgaggtcc atatgtgttg actgcgcata gtgtcggctc
     3481 cttacattag catccttgaa ggacgcggcc agactactgc tctatctcgc cagcgtcgtg
     3541 gtgctgggtg cacttgcggc cccgccgctt ttctggttcg gccaatacct catcgcgcag
     3601 cgcattttgc cgggtctcgc tggttttgat ttcgagactt acttccatcg cgcgctgctc
     3661 atcgccgcgc tcgtcctgct ttggccgctc ctgcgcgcgg tgcgggtgcg cggctggcgc
     3721 gatcttggac tggagaaaaa cgcgcacgcc ggggctgatg tcacggccgg ctttgtcatc
     3781 gcggcggttc ccctgctctg ctgcggtgcg gtcctcgtcg cactgcacat ctattcgccg
     3841 cgacaggcgt ttctctggca caagatgccg gacgtgctgg tcgccgccag cgtcgtcccg
     3901 gtcctcgagg aattgctctt tcgcggattc atcctcggtc tgttgctgcg cagtttttcc
     3961 cggctcggcg cgctcattct cacttcgacg cttttttcca tcattcattt cctgaaagcg
     4021 cccgagctga ccacgcctaa cgacgccgtc gcctggtcct ccggcttcgt ctcgatcggg
     4081 cattccttct ggcagtttgg cgatccggtc ctgctcgccg ccggtttcct gactctcttc
     4141 gcgatcggct gcatcctggc cgacgcgcgg cttcagaccc gctcgctctg gctctcaatc
     4201 gggctgcacg ccggatggat cttcgccaac ggcgccttca gtcgagcgac gcgccgtgcc
     4261 gcgatcgcgc tcccgtgggt cgggaaagac ttgctcgtcg gaatcgtgcc gctcatcatc
     4321 gccctggcca gctgggcgct gatgcgcggc tgggcgagat atgctgatcc gcgcgctccc
     4381 caatccgttc cgcgggctcg ttagtctctt ctatccggcc ttctgcgcgg tttgttccgg
     4441 cgcggtggag ccgcgcgaag atctctgcgc ggtttgcgcc gggcaggcga cgcgcatcgt
     4501 ccctcccttt tgcgcaaaat gttctcagcc cttcgacggc gcgattaccg gcaccttcac
     4561 ctgcgcgaac tgccaggacc gcgtcctcca tttcgaggcg gcagtttccg cttatcgcag
     4621 ccggggcgtg gtgcgcaagg tgatgctcga tttcaaatac ggccgccaga ttcatctccg
     4681 gcatctcctc ggccgctggc tctcggaaac gctggccgat ccgcgcctcg ccggccgcca
     4741 ctttgatctc atcgtccccg tgcccctgca tccggcgaaa aaacgcgagc gcggtttcaa
     4801 ccaggccgag ctgctcgccg ccgtcctccg ccgccgctcc ggcgtgcgcg ttgagaatgt
     4861 gctccagcgg acccgctaca ccttgacgca gacgcaattc gaccggagcg agcggatgga
     4921 aaatctccgg ggcgcctttc gtttacggcg tggcagcaac gtgcaagatt tgcgcatgtt
     4981 attagtcgat gacgtcctga ccaccggttc gacgttaagt gaatgcgcct cggttttaaa
     5041 gaaagccggc gcgcattcgg tccacgcggc gaccgccgcc cgcggctaaa ccatggccat
     5101 ttttaaaaag ccagcgctca agactgaaag ccgcaagaag cgcgagatgc cgccgggcct
     5161 ctggcagaag tgcgacggtt gcggcgaggt cgtgcacgaa atcgaactgg tggagaacga
     5221 ccgcgtctgc ccgcgctgcg attaccattt cacgcagacg gccaaggagc ggatcgcgaa
     5281 gttgtgcgat cccgacagct tcgtcgaact cgatgcccgc ctgacctcga tcgacacgct
     5341 caagttccag ggcatggcga gctacaagga tcggctgcgc aaataccagg acaagaccgg
     5401 cctggtcgac gccgttctca gcggccacgc gctcatcgaa ggctacaagg tggcgatcgc
     5461 ggtgatggat tttagcttcc tcgcggccac gatgggctcg gtggtcggcg agcgcatcac
     5521 gcgggtcatt gaatacggca ccgcccaccg tgccgcagtc attatcgtcg cggcctcggg
     5581 cggcgcgcgc atgtacgagg gcatgctcag cctcatgcaa atggcaaaga caagtggcgc
     5641 cctcgccctc cacggggaga gacggctgcc gtttatctcc gtcctcacca atccgaccac
     5701 cgccggagtg atggccagtt tcgcgtcgtt aggcgacgtc atcatcgccg aaccgaaagc
     5761 gatggtcggc ttcgccggac cacgcgtgat caaggaaacg acgcaccagg atttgccgga
     5821 gcgcttccag accgccgagt ttctggagga acacggtttg attgatctca tcgtgcaccg
     5881 caaaatgttg cgcgcgcagc ttgcgcaact gcttgcctac ttctcctcga cgcgttgaac
     5941 gtgggagagg cctccgtgcc gcgatctgcg ggagccggcc tccggccgcg aaccccctac
     6001 gaggaggccc tcgcctggct tttcggcacc cagcgtttcg ggatcaagct cgggctcgaa
     6061 aacagccggc gtctcttcgc cgcgctcggc gtgctctcgc ctaacgacag gatcatccat
     6121 gtcgcgggca cgaatgggaa aggttccgtt tgcgccttgc tcgattcgat ctgccgcgcg
     6181 gccggttatc gctccgccct ctttacctcg ccgcacctcg tgactttccg cgaacggatc
     6241 cagttgaacg gcgaactgat ctccgaagag gaagtggcgc gcggcctaac gaaaatccgt
     6301 gaactggtcg ccggctggga tccgcacccg acctttttcg aaattaccac cgggctcgcg
     6361 ctcgattatt ttcggagacg cgaggccgaa atcatcgtgc tcgaaacggg catgggcggc
     6421 cgtctcgacg cgaccaacgc gacgcaaccg gttgtttccg tcctcacccc gatcgattac
     6481 gaccaccaga aatggctcgg cgaaaccctg acggaaattg caggcgaaaa agccggcatc
     6541 atcaaagccg gcattccggt tgtttccgcc cggcaaccgg aggaagccgc ggccgtcatc
     6601 cgggcccggg cggccgaatg cggcaccccg ctcgctttcg ttaggcaacc ctttgaccgc
     6661 ttcccgatcg ccttgcacgg gcggcatcaa aaggaaaacg ccgcgctcgc catctccgcg
     6721 ctgcacaccg cgcaaatccc gatcgacgac gcggccctcg aacgcggact ggccacggtc
     6781 gtgtggccgg cgcggttcca gcaatggaac gcgcgcacaa ttatcgacgg cgcgcacaac
     6841 ccggccggtg cgcgcgttct cgccgagacc tggcggcaaa tcttcggtga tcggcgggcg
     6901 acgattttgc tcgccgttct cctggacaag gatgtcgcgg gcatctgctc cgcgctggaa
     6961 ccgatcgccc gtcgcgcatt gctcccggcg atccggagcg aacgcgccct gccgccggaa
     7021 gaacttcgcc taacgatttg cgatcggctc cccgggctgc cagtggccgt cgcgccttcg
     7081 ctcgccgctg cctgggagga agcgcggcgc gacgccgctc cgatcctgat ttgcgggtcg
     7141 ctgcatttcg ccggcgaggc gctcgccttc ctgagcggcg agccggctgc cttcgaagag
     7201 tgcttgcagt gaggtcgacg cttcgcaccg cggagccgag aggcaaattc gtcacgattc
     7261 cgtccgttcg acgcccgtct cgcgctgcgc acagcgacgc gtctacaggc gtcgcaacgc
     7321 cccagcgcaa gtaaccgcta ataaatcgca acccaccggc agcggaacaa tcggctttat
     7381 cctagctttc cagtgtgaaa ttgatgacgg cgaccgccaa catcgtccat ctttcgccgc
     7441 cgacgcgcgc ccggacatgc gatcagaggt cgcccgccgt gagcgcgcga cgtttccttc
     7501 gtcctctgct cgggtgcggc ttggtggtgt cgacgctttg tgtgctgggc gtgctcgtct
     7561 ggcagggaat taccgcgcat ggcgcgcccg acccgaccca gccgcagacc agcccggcga
     7621 tggcgtttct ggatatcggc gtccttgttt tccgcgaagg cctcgagtgt atcctcgtgc
     7681 tcgcggcgct caccgccagc atgaccggcg acaaggcgat ccaccgccgg cccgtcgccc
     7741 ttggggccgg actgggattt gtcgccaccg gcgtcacctg gctcatcgcc atcgggatca
     7801 tgaaacaact catcgacagc gtgccggcac tcgatctgca ggcggcgacg gggctgctcg
     7861 cggtcgtcgt gctggttgtg atcatgaact ggttcttcca caaaatttac tggggcggct
     7921 ggattcgcgc gcataaccgc gccaagaaaa acctcctcgc cagcgcaacc gacaatcacg
     7981 gctcgcagcg aaaattgctt tggggcctgg tcctgctcgg cttcacctcg ctttatcgag
     8041 aaggcttcga ggtcgtgctt tttctgcaaa gttaccggct gaaattcggc aacagtgtcg
     8101 tgttggaagg cgctctcgtc ggtctcatcc tctcgggaat ggtcgccgtt ctgaccttcg
     8161 ggctgcaacg gcgtctgcct tatcgccaga tgctcatcac caccggcgtg ctcctcggcg
     8221 gcgtgcttct cgtcatggtg ggcgagcaag cccaggaaat gcaacttgcg cattggatct
     8281 ccaccacccc gattcccgcg ctcgaaccca tcgtgcggcc gtggatggga atgtggtttg
     8341 ccgtgttccc gaccgtcgaa accctcgccg ctcaggcaat cgccgcgatc gtcgtgatcg
     8401 gctcctatta cgccgcgcgg cggatgggtc gggcggcggc ggcggccgcg gttgctttgc
     8461 cgaggaagaa gttgctcggc gggtaggtcc gcacacgctc ccgctcttgc gaatgggtgg
     8521 ctggcgttga agccatccct gggcagtgca actgccggga tggtgcgttc ccaagtgcaa
     8581 cttgggaacg agatgcgaga tgaccgttcg tcccacgacg ccaaaggcca cggccgaaac
     8641 gggacgctga ttccgcgggc taacgtcatg cccctcgttc ccaagttgca cttgggaacg
     8701 cgcttgtctt cgaagttgca cttcgccacc ctctcctcat gcgtagtcgc taccgcatcc
     8761 tcgagaagca gcacgcgcat ttcgtcacct gcacgatcgt ggtctggctc cccatcttca
     8821 cgaccgcggc ccggtgcgac attctggtgg aggcgctgac ctattgccga gcacacaagg
     8881 gacttgagat ctatgcctgg gtcatcctcg acaatcattt ccatgccatc ctcggcgcgc
     8941 cggaccttgc gcgcgtcatg gccgacctca agcgccacac cgcgcggcgc ctggtcgagc
     9001 aactagagac tgaaggctgc gtctggctgt tgcaccagtt gcagcactac cggctcgcgc
     9061 acaagaacga gagcgtgcat caggtgtggc aggagggatc gcatcccaag gcaatgatga
     9121 gcgacgcgat gatggagcag aaactggaat acttgcatca caatccggta aagcgcgggc
     9181 tggtctctcc tcgcccgggc attggcgtta ttcctcggcg cacgagtggc tcccgggcgc
     9241 gctcccgctc ttgcgggtgg attcctggcg ttaaagccgt cccgaggtag tgcaactggc
     9301 gggatggtgc gttcccaagt gcaacttggg aacgagacgc tagacaatcc ggtaaagcgc
     9361 gggctggtct cctcgcccga gcatcggcgc tattcctcgg cgcacgagtg gctcccgggc
     9421 gcgctcccgc gcttgcgggt ggatgcctgg ggttgaagcc gtcacggcgc agtgcaactg
     9481 ccgggattg
//
LOCUS       DATMNS010000123         2986 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5187059, whole genome shotgun sequence.
ACCESSION   DATMNS010000123 DATMNS010000000
VERSION     DATMNS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5187059"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VNW28_04550"
     CDS             <1..237
                     /locus_tag="VNW28_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09226.1"
                     /translation="SILKIADASLRLEALPEARHGVTFRNITLLPFLAHVPTLPKLPR
                     TRIVPLENLASLPFSSATRKIASAAASHKPPLAD"
     gene            300..>2986
                     /locus_tag="VNW28_04555"
     CDS             300..>2986
                     /locus_tag="VNW28_04555"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HXA09227.1"
                     /translation="MASTAQPITQTASPLPAVQRYFEVSLFLLVATGLLAIISTGKLD
                     IFSTIITSLALAYKAVRTWRACGPELSSRVATGMVLAYFLFFPFDIWVLSRDLAQGAP
                     NTALYAALLAAIHLLIFATLVRLFSARTNRDYAFLAVLAVAAMLASAILTVETGFLVA
                     LTIFLILAVSTFVALEIRRSSVGAVTPPFDAGSPTALQLNRALGVTSVLVAVSALLLG
                     GVIFFLIPRFTTGYLSALNLQPTLMTGFSDNVELGEIGRIKQNTAVVMRIHVEGDPTQ
                     ASEIHWRGIVLTDFDGQRWFTPAGSETILTGDEDGLYSFASPTLPSADFHQLHYAILM
                     EPIATDAIFVAPRPENLHGRFFDAVTRPGMPPRRGYLLVDRTGSVFNPSHNEMKIRYE
                     GTSALPIIPPSVLRQASAEYPKAISDAYLQLPALDPRTRDLAAQITSKFSNEYDKAAN
                     VELYLKAHYAYSLDLTGPRAADPLANFLFVTRSGHCEYFASAMTVLLRAAGVPARYVT
                     GFLPGEYNDLGGDYIIRQSDAHAWVEVYFPGYGWITFDPTPAGDSKSAGVFARMSLYW
                     DWFQYAWSEWIVNYDFGHQIALGQGIQKSSSSWSDRAQQFYHRKQDEAMQFLLALDRR
                     TEASPYFLPGVLVFLIALLIFMRGRPVIGYLFARWTLRARRGGNLTASLAVFEYREML
                     RLLEKHGWTKRESQTALEFASAIPATNISQHVAQMTELYHSARFGNHPARVDQMSSLL
                     RSIRDLLHSRKSGSAAVGITLLLLTSFSLAGLVRAQGTSPSLSGDNSDWWSIMKEDVP
                     RSPRQPEHRLLAATNFKILGLSINEDDLFAIGDFLGRAASKFGRAEEVDRGDAASGRA
                     QICYKSGEGSQRSYLVFEGGETDLAFYLFSGGPDWGGNQ"
BASE COUNT          540 a         1006 c          767 g          673 t
ORIGIN      
        1 tcaattctca aaattgcaga cgcatcactt cgcctggaag cattgcccga agcgcgccac
       61 ggcgtcacct ttcgcaacat cacgctgctg ccttttctcg ctcacgtccc gaccctcccg
      121 aagctgccgc gcacgcgcat cgtcccactc gaaaatctcg cgagcctgcc gttctccagc
      181 gccactcgca aaatcgcctc tgctgccgca tcgcacaaac cgccattagc cgattagcgt
      241 ctcgcgcgcc ctccgaccgc gaacgaccag ccgctcggat gttagcatcg taaagcgcca
      301 tggcctcgac cgcacaaccc atcacgcaga ccgcatcgcc gctgcccgcg gtgcagcgct
      361 atttcgaggt atcgcttttt ctgctggtcg ctaccggact actggccatc atctcgaccg
      421 gcaaactcga tattttctcc accataatca cgtccctcgc actcgcctac aaagccgtgc
      481 gcacctggcg cgcctgcggc ccggagcttt cctcccgtgt cgccactggt atggtgttgg
      541 cctactttct atttttcccg ttcgatattt gggttctctc gcgcgacctc gcgcagggcg
      601 cgcccaacac cgcgctctac gctgcattgc ttgcagccat tcacctgctg attttcgcca
      661 cgctggtgcg ccttttctcc gcgcgcacca atcgcgacta cgcgttcctc gcggttctcg
      721 cggttgcggc catgctcgcc tccgcaattc tgaccgtcga gaccggcttt ttggtcgcgc
      781 tcacgatttt tctgatcctt gcggtctcca ctttcgtcgc gctcgagatt cgccgcagct
      841 cggtgggtgc ggtcacgcct ccgttcgacg cgggctcgcc aacggccttg caactgaatc
      901 gcgcgcttgg cgttacctca gtcctcgtcg ctgtaagcgc gctgctgctc ggcggcgtga
      961 tttttttcct gattccgcgc ttcaccaccg ggtaccttag cgcgctcaat cttcagccca
     1021 cgctgatgac cggcttttca gacaatgtcg agctcggcga aatcggaagg atcaaacaga
     1081 acacggctgt ggtgatgcgc attcacgtcg aaggtgatcc cacgcaagct tcggagatcc
     1141 actggcgtgg catcgtcctc acggatttcg atggccagcg ttggttcacg cctgccggct
     1201 cggaaaccat tctcactggt gacgaggacg gcttatactc cttcgcctcg ccgacgcttc
     1261 cgtccgccga tttccaccag ctgcattatg cgattctgat ggaaccgatc gccaccgacg
     1321 ccatcttcgt ggctccccgc cccgaaaacc tgcacggccg cttcttcgac gcagtcacgc
     1381 gccccggcat gcctccgcgc cgcggctacc tgctcgttga ccgaaccggc tcggtcttca
     1441 atccctctca caacgagatg aaaatccgtt acgagggcac ttccgcgctg ccgattattc
     1501 cgccatctgt cttgcgccag gcatccgccg agtatccgaa agcgatcagc gacgcctatc
     1561 tccaactgcc ggcgctcgat ccgcgcacgc gcgacctcgc cgcgcagatc acctccaaat
     1621 tttcaaatga atatgacaag gccgccaacg tcgagctcta cctgaaagcg cactatgcct
     1681 acagcctcga tttgactggc ccgcgcgcgg cagatccgct cgcgaatttt ctttttgtga
     1741 cccgatccgg ccactgcgaa tattttgcgt ctgcgatgac ggttcttctg cgcgccgccg
     1801 gagttcccgc gcgctatgta accggattcc tccccggcga gtacaacgat ctcggcggcg
     1861 attacatcat tcgccaaagc gacgcgcacg cgtgggtcga ggtctatttc ccgggctacg
     1921 gctggataac gtttgatccc actccggcag gagactcaaa gagcgccgga gtattcgcgc
     1981 ggatgtcgct ttactgggat tggttccaat acgcctggag tgaatggatc gtcaactacg
     2041 atttcgggca ccagatcgct ctcggccagg gcattcagaa atcatcgagc agttggagcg
     2101 atcgcgccca gcagttttat caccggaaac aggacgaagc catgcagttc ctgctggcgc
     2161 ttgaccggcg caccgaagca tcgccctatt tcctgcccgg cgtgctcgtg tttctgattg
     2221 cgctgcttat ttttatgcgc ggccgtcccg tgatcggcta cttattcgcc cgctggacgc
     2281 ttcgcgcgcg ccgcggcggc aacctcaccg cctctctcgc ggtattcgag taccgcgaaa
     2341 tgctgcgcct gctcgagaaa cacggctgga cgaaacgcga gtcgcaaacg gctctggaat
     2401 tcgcgtccgc cattcccgcg acgaatatat cccagcatgt cgcccaaatg accgagctgt
     2461 accactccgc ccgcttcggc aatcatcccg cccgcgtaga tcaaatgtcg tcgctcctcc
     2521 gctccatccg cgacctcctc cactcccgca aatccggaag cgcagcagtg ggtattactt
     2581 tgctattgct cacgtccttc tcactagctg gtctcgttcg tgctcagggc acctcgccca
     2641 gcctctctgg cgacaattcc gactggtggt cgataatgaa agaagatgtg ccgcgatcac
     2701 cgcgtcagcc ggaacatagg ctcttggcag caaccaattt caaaattctc ggcctttcta
     2761 tcaacgagga tgatcttttt gcaatcggtg actttctagg cagagctgcc tctaaattcg
     2821 gtcgggccga ggaagtagac cggggcgacg ctgcttcggg gcgtgcccaa atttgttata
     2881 agtcgggaga gggatcgcaa cgatcgtatc tagtctttga aggaggcgaa acggatctcg
     2941 ctttttattt attctccgga ggtccagact ggggaggaaa ccagta
//
LOCUS       DATMNS010000124         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5191353, whole genome shotgun sequence.
ACCESSION   DATMNS010000124 DATMNS010000000
VERSION     DATMNS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5191353"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VNW28_04560"
     CDS             <1..195
                     /locus_tag="VNW28_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09228.1"
                     /translation="DWATKKERVAVRHRGDADVLARSVGELVAPAQARPAAWCSDLYL
                     TRQCPPKEYPPMGEEIESGS"
     gene            375..1163
                     /locus_tag="VNW28_04565"
     CDS             375..1163
                     /locus_tag="VNW28_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09229.1"
                     /translation="MELAKNSLARLRTFRVDEEQRRQLYFSPFIQNLRDERDKANGVA
                     EQELSKRYSWEQLAWRREIDPIQRRRVLASVNDKKGYDTLWCTVNDLQRKRLKQEATR
                     HATGLSRAYTFDEDGRYALLSAVMEREAGPLGFSADKLRSRIYSPVFWKQVTESWGLL
                     WTLEDPDGMFFHNSSEGHFRPALKIGNAALRDPEKVEAGELLSIRYQSIIPGFYNALP
                     YLLGRRRARDNNQGTPLFIRSDRTNHCGSNKDRFAKLIMSAPVC"
     gene            complement(1186..1860)
                     /locus_tag="VNW28_04570"
     CDS             complement(1186..1860)
                     /locus_tag="VNW28_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09230.1"
                     /translation="MNSNDRLSQGDLEDLLPWHAAGTLDANEAAEVERALACDPELAR
                     RFALVREELTETILLNESLGAPSARAMETLFRAIDQDRRVVRRAAFTPSLGARIAEFF
                     SPRALAWTAAAAAVIVVLQAGVITRMALEQRDGGTFETASAPVTRGLSIGSYALVRFA
                     PQASMADVNKFLDDRGAAIVDGPKPGGLYRVRVAEGSVSRPELARLVKELEAAKTIVS
                     FAAATE"
     gene            complement(1857..>2450)
                     /locus_tag="VNW28_04575"
     CDS             complement(1857..>2450)
                     /locus_tag="VNW28_04575"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXA09231.1"
                     /translation="ATNPTSRSLSANSAASDEVLVGRIAQGDRLAMQVLFARHHVRVY
                     RFVLRFVRNEASAEDLISEVFLDVWRQADRFEGRSAVSTWLLAIARFKALSSMRRKPD
                     EELDEEAAGAIEDTSDTPEVSLQKKDKSGLLRKCLEQLSREHREVIDLVYYHEKSIEE
                     VAEIVGIPEATVKTRMFYARKKLSELLKAAGLDRGWP"
BASE COUNT          490 a          739 c          721 g          500 t
ORIGIN      
        1 gactgggcca caaaaaagga gcgtgtcgcc gtccggcacc gcggtgacgc cgatgttctc
       61 gcccgctcgg tcggcgaact cgtggcgcca gcccaggcaa ggcccgcggc gtggtgcagt
      121 gatctgtatt tgacgcgcca atgcccgcca aaggagtatc ctccaatggg tgaagagatc
      181 gaaagtggct cctaaactat cgtttgcgca agccaacgct atctgcttgg gacttcttcc
      241 gatcggcaac tcggtattcg acatcatgaa tcgactccca gggggcagga ttcgccgctt
      301 ggctgcccct ccatgaattt ctcgagtcgt tcgcagagac acctgacgag cttgagttgc
      361 accgcaattg cgttgtggaa ttggccaaga actcgctggc acgacttcgg accttccgag
      421 tcgatgaaga gcaacgccgg caactttatt tttcgccttt tattcaaaat ttgagagacg
      481 aacgggataa ggcgaacggc gttgcagaac aggagttgag taaacgatat tcgtgggaac
      541 aactcgcgtg gcgacgcgaa atcgatccga ttcagcgacg acgagttctt gcctcggtca
      601 atgacaagaa aggttatgac acgctatggt gtacggtgaa cgacttgcaa agaaagcgct
      661 tgaagcagga ggcgacacga catgccacag gcctttcgcg agcatacacg ttcgatgagg
      721 atggccgcta cgctctttta tctgccgtta tggagcggga agccgggccc ctaggattca
      781 gtgctgacaa gttgaggtcg cgtatctatt caccggtctt ttggaagcaa gtgactgaaa
      841 gctggggcct cctctggaca ttggaagacc ccgacggcat gtttttccat aattcgtctg
      901 agggacactt tcggccggct ctcaaaatcg gtaacgccgc attgagggat cctgaaaagg
      961 tcgaagctgg cgagctttta tcgattcgct accaaagcat cataccgggg ttttataatg
     1021 cattacctta ccttctcggg cgtcgacgag ctagagacaa taatcagggc acacctttgt
     1081 ttatacggtc tgatcgcacc aatcattgtg gaagcaataa ggaccgcttt gcgaagctca
     1141 ttatgagcgc gcccgtgtgt tgagggcggg gcccggcccg gaaagttatt ccgtcgccgc
     1201 agcgaaactc acgatggttt tggcggcttc caactctttc acgagacggg ccagctccgg
     1261 ccgcgacacg ctgccctctg cgacccgcac ccgataaagg ccgcccggct tcgggccgtc
     1321 gacgatcgcc gcaccgcggt cgtccaggaa cttgttcacg tcggccatcg aggcctgcgg
     1381 cgcgaaccgc accaacgcat aggatccgat gctcagtccg cgcgtaaccg gagcggatgc
     1441 cgtttcgaac gtaccgccat cgcgctgctc cagcgccatt cgtgtgatca cgccggcctg
     1501 cagcaccacg atcaccgccg cggctgccgc ggtccaggcc aaggcgcgcg gggagaagaa
     1561 ttccgcgatc cgggcgccga ggctcggagt gaaggctgcc cggcgcacga cccggcggtc
     1621 ctggtcgatg gcgcgaaaca gcgtctccat cgctcgcgcc gagggcgccc cgagggactc
     1681 gttgagtagg atggtttcgg tcagttcctc ccgcaccagg gcgaagcggc gggcgagctc
     1741 ggggtcgcag gcgagcgcgc gctcgacctc cgcggcctca ttggcgtcga gggtgccggc
     1801 ggcgtgccac ggcaacaagt cttcgaggtc gccttgggag agtctgtcgt tcgaattcat
     1861 ggccaacctc tatccaatcc cgctgccttg agcagctctg agagtttctt gcgcgcgtag
     1921 aacatgcgcg ttttgacggt ggcttccggg ataccgacga tctcggcgac ttcctcgatg
     1981 gatttctcgt ggtaatagac gagatcgatc acttcccgat gttcccgcga aagctgctcc
     2041 aggcactttc gcaacagccc gctcttgtcc tttttctgca acgagacttc cggtgtgtcc
     2101 gaagtgtcct cgattgcccc cgccgcttct tcatccagtt cctcgtcggg cttgcgccgc
     2161 atcgaggata acgccttgaa ccttgcgatg gccaggagcc aagtcgatac cgcgctccgt
     2221 ccctcgaacc gatccgcctg gcgccacacg tccagaaaca cctcgctgat caggtcctcg
     2281 gcactcgcct cgttgcggac gaaacgaagc acgaaccggt acacccgcac atgatggcgc
     2341 gcgaacagaa cctgcatcgc gagccggtcg ccttgggcga tcctaccgac cagaacttcg
     2401 tccgaggctg cagagttcgc gctcaacgat cggctcgtag ggtttgtcgc
//
LOCUS       DATMNS010000125         3846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_898768, whole genome shotgun sequence.
ACCESSION   DATMNS010000125 DATMNS010000000
VERSION     DATMNS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898768"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..702)
                     /locus_tag="VNW28_04580"
     CDS             complement(<1..702)
                     /locus_tag="VNW28_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HXA09232.1"
                     /translation="MRKTRAPISVRSGQIEFLKLNTERWEWRDAYQWLLGLTWSKFAA
                     FVGAVYVALNLLFAALYTAKEGCVAGMRPGSFLDAFFFSVQTVATVGYGTMAPSGLYG
                     NILSTIEIMIGIFLVAVMAGLIFVRFSRPTAHIVFSKSIVIAPLNGQPTLMLRVGNLH
                     QQSMVEAEFRIFFNRDEPLAEGGDFRFFYNLKLQFDRLPTFPAALTLRHVIDKHSPLY
                     GATPESLEAGRVVLMA"
     gene            complement(829..2301)
                     /locus_tag="VNW28_04585"
     CDS             complement(829..2301)
                     /locus_tag="VNW28_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXA09233.1"
                     /translation="MSDSPDDSERLADKGARKVLLAAAAALVSAALLYLGTGLHPIYW
                     LLWVAPIPVLMIAARLRAGVAFLLAAVTWLIGEMNLWNYLAHGLELPLPIILFVLFVP
                     AIAFGVGVLFMRGFLRRGSLFRAALALPAYWVAYEYLTEVGSPHSTFGNLAYTQMNAL
                     PIIQIASLTGIWGISFLVFLFAGTIAVLLGGAGEPRRRRALAITVASFICAVFLYGGW
                     RLRSNSSAPFVTVTLIARDVPMSVYLGPEAQTLALLHDYADEIRRATPAGTQVVVLPE
                     KIGRVSESALPQVDALFSSAAAATHSAIVLGLVRKTPSAAFNSSRFYSAAGKLVANYD
                     KHHLLPGVEPEKPGDKRVVVDQPSGRWGLQICKDMDFPQLSREYAGAGASLLLVPAWD
                     FQLDGWLHARMAVLRAVENGFALARAARSGLLTLSDNRGRILAEAATEPGRFVSLTGK
                     VNIPRAETFYTRTGDWFAWTCVAIFGALLVSSFLPRPRPR"
     gene            complement(2405..2725)
                     /locus_tag="VNW28_04590"
     CDS             complement(2405..2725)
                     /locus_tag="VNW28_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HXA09234.1"
                     /translation="MAHVVTNKCVGCKFTDCVVVCPVACFYELEGQLIIHPEECIDCM
                     ACVDECPVHAIYAGEDVPPEFQADIEFNATEARRVQESGQGAIETKKDPLPTAEARKA
                     ELGY"
     gene            complement(3147..3425)
                     /locus_tag="VNW28_04595"
     CDS             complement(3147..3425)
                     /locus_tag="VNW28_04595"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXA09235.1"
                     /translation="MHRVVEELFYRGYAIERLQSLGLNRWVAGIIPLLIFAFGHGTNG
                     WANVVLALALGALLTVFYLWRRDLVANMVGHFTIDLLSVLLPRFLSHS"
BASE COUNT          905 a         1137 c         1150 g          654 t
ORIGIN      
        1 ggccatcagg accacccggc ctgcttcaag tgactccggc gtcgcaccgt aaagcgggct
       61 gtgtttatcg atgacgtgcc ggagcgtgag cgcggcggga aacgtcggta gccggtcgaa
      121 ctggagttta aggttgtaga agaagcgaaa gtcaccgcct tccgctaatg gctcatcgcg
      181 attgaaaaag attcgaaatt ccgcctcgac catgctctgc tgatgcaaat tgccgacgcg
      241 cagcatgagg gtgggctgac cgttcaaagg cgcgatcaca atggatttgc tgaagacgat
      301 gtgcgcggtg ggccgcgaaa accggacaaa gatgaggccg gccatgacgg caacgaggaa
      361 gatgccgatc atgatttcga tggtgctaag gatattaccg taaaggccac tgggagccat
      421 ggtcccgtag ccgacggtgg cgacggtctg cacgctgaag aagaaggcat cgaggaagga
      481 gccgggccgc attccggcca cgcaaccctc cttggccgta tagagcgcgg cgaaaagaag
      541 attgagcgcg acgtaaaccg cgccgacaaa ggccgcgaac ttcgaccagg tcaacccaag
      601 cagccattga taggcgtcgc gccactccca cctctcggta ttgagcttga ggaattcgat
      661 ctggcccgag cggacggaaa ttggagcgcg cgtctttctc ataaatagag tgtaaccatt
      721 ttctgcgcgc gcgcagatgt tcgccgacgg ccgcacggtt caaacatgct ccgaaatggt
      781 gatcggcctg tggcggactg aagcgctgaa atcaaccgga ccaaaaaatc atcgcggccg
      841 aggtcgcgga agaaagctgg agacgagcaa ggcgccaaag atcgcgacac aagtccaggc
      901 aaaccaatcg ccggtgcgcg tgtaaaacgt ttctgcgcgt gggatgttta ccttcccggt
      961 gagcgagacg aagcggccgg gctccgtcgc cgcttcggcc agaatgcgac cgcgattatc
     1021 gctcagagtc agaagcccgc tgcgcgccgc acgcgccagc gcaaagccat tctcgaccgc
     1081 gcgcaggacg gccatgcgcg cgtgcaacca gccatcaagt tggaagtccc acgccgggac
     1141 gagcagcaag ctcgcccctg cgccggcata ctcgcggctg agctggggaa aatccatgtc
     1201 cttgcaaatt tgcaatcccc aacgcccgga cggctgatcg actacgactc gtttatcgcc
     1261 gggtttttct ggctcgaccc cgggcaaaag atgatgcttg tcgtaattcg ccaccagctt
     1321 tcccgcggcc gaataaaagc gggaagaatt gaaggcggcg gagggcgttt tccggaccag
     1381 accgagaacg atcgccgaat gggtcgcagc cgcggcggaa gagaagagcg cgtccacttg
     1441 cggcaatgcg ctctcactga cgcgcccgat tttttccggg agcacgacga cctgtgttcc
     1501 ggccggcgtc gcgcggcgga tttcgtcggc gtagtcatgc aggagtgcca gggtttgtgc
     1561 ttctgggccg agatagacgc tcatcggcac atctctggcg ataagtgtca ccgtgacgaa
     1621 gggagcggaa gaattcgagc gcaaccgcca tccgccataa agaaacaccg cgcaaatgaa
     1681 gctcgcgacc gtgatcgcca gcgcgcgacg acgccgtggt tctccagcgc cgcccaacag
     1741 gacggcgatc gtgccggcga agagaaagac aaggaagctg atgccccaga tgccagtgag
     1801 cgaagcgatc tgaatgatgg gaagcgcgtt catttgggtg taggccaggt ttccgaaggt
     1861 gctatgcggc gagccgacct cagtgagata ttcgtaggct acccagtaag cgggcaaagc
     1921 aagcgccgcc cggaagagcg atccgcggcg gagaaagccg cgcatgaaca gaacccctac
     1981 accgaaagcg atcgccggga cgaacaggac aaacaggatg atcggcagcg gcagctcgag
     2041 cccatgggcg agatagttcc ataggttcat ctcgccgatc aaccaagtaa ccgcagcgag
     2101 gagaaaagcg acaccggcgc ggagtcgtgc cgcaatcatg agcactggaa ttggcgcgac
     2161 ccaaagcaac cagtaaatgg gatgcagccc ggtgccaaga tagagcaagg cggcagacac
     2221 gagcgcggcg gcggccgcga gaagaacctt acgcgccccc ttatccgcta accgctcact
     2281 atcatcgggc gagtcggaca ttcattgaat gtcgcatcga acctgggaca gagcgagttc
     2341 ccaaaccagg ttgccggggt cgcgacctgt tcgccctggc ctcacccggc gcctgcgagc
     2401 aagttcagta acccaattcc gctttcctcg cctccgccgt gggcagagga tcctttttcg
     2461 tctcgatggc gccctggccc gactcttgca cccgcctcgc ctccgtcgcg ttgaattcaa
     2521 tgtccgcttg aaactccggc ggcacgtcct ccccggcgta gatggcgtgc accggacatt
     2581 catccacgca agccatgcaa tcgatgcatt cctccgggtg aatgatcagt tgaccttcca
     2641 actcgtaaaa gcaggccacc gggcagacca ccacacaatc ggtgaattta caccccacac
     2701 atttattggt aacgacatgc gccataaatt atttctctcc caggttcatc gggcttacgg
     2761 aagcaggctc gacgacgcgg tgcaagttgc caacgcgttt ttcagaaaat acagtcgatc
     2821 gcgggttgcc ctgatttgac cgacaatcgg ctggggacca aaactccggg agaagccacg
     2881 caaagacacc gtcaaggaag ccgaccatcc gctctgcaaa gcaaaatagg taccaacatc
     2941 ccccgccctc tggccgtgct ttcccgcgtt cctgattaat cttcctgagc ctcccagttg
     3001 tccgattcca ggatttgggt cgccggggcg cgtgatcgcc gggccggcga cgccgccaac
     3061 gacctgcata cgccgcgcaa accgcagacg acgaggtttg gccgaaggtt cggccgaccg
     3121 agaaacgatc tatccgagct tcgttgttag gagtgggaaa ggaatcgcgg cagtaggacc
     3181 gagagaagat cgatcgtgaa gtgaccaacc atattggcga cgaggtcgcg ccgccagaga
     3241 tagaagacgg tcaagagtgc gccgagagcc agcgcgagaa ctacgttggc ccaaccgttg
     3301 gtcccgtgcc cgaaggcgaa gatcaagagc ggaatgatac ccgccaccca acggttcaac
     3361 ccgagcgact gaagccgttc gatcgcgtag ccgcgataga aaagttcctc gacgacgcgg
     3421 tgcaagttgc caacgcgttt ttcagaaaat acagtcgatc gagggtcgcc ctgatttgac
     3481 cgacaatcgg ctggggaaca aaactccggg agaagccacg tgaagacacc gttaaagaag
     3541 ccggccatcc gctctgcaaa gcaaaatagg taccaacctc ccccaccccc tggccgtgct
     3601 ttcccacgtt cctgattaat cttcctgagc ctctcagttg cctgattcca ggatttgggt
     3661 cgccgcggct cgtgatcgcc gggcggcgac gccgccagcg acttgcacac gccgcgcaaa
     3721 ccgaagccga cgaagtttag ccgaaggttc ggccgaccga gaaacgatct atccgagctt
     3781 cgttgttagg agtgggaaag gaatcgcggc agtaggaccg agagaagatc gatcgtgaag
     3841 tgacca
//
LOCUS       DATMNS010000126         3140 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2688310, whole genome shotgun sequence.
ACCESSION   DATMNS010000126 DATMNS010000000
VERSION     DATMNS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2688310"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            80..499
                     /locus_tag="VNW28_04600"
     CDS             80..499
                     /locus_tag="VNW28_04600"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HXA09236.1"
                     /translation="MKWITRKGVKVDRVACPWLIRKFVDPDAEFVFLPPETDWASVKD
                     GTVYDVPNCELGHRGEDVSFDSILKKYELTDPALRLLAEIVRAADSHPSNPHPAGEGL
                     RWVAHGFSALGLSDHEILAREFVVYDGLYAECQRRVR"
     gene            516..1736
                     /locus_tag="VNW28_04605"
     CDS             516..1736
                     /locus_tag="VNW28_04605"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXA09237.1"
                     /translation="MKNRRFLAEFFGLRRNLVILLIAIFAIGAGEELWMRFIPKYLEA
                     LGATVFIIGTFDAIRTLLGAIYAYPGGLVVDRWGFRHAFIAFNLLSIVGYAVVLLIPH
                     WGAVLCAMFLFLSWTCFSLPATFSLIAETLDANRHSMGIGMQSVIKRLPIMIAPFLGG
                     VLIDRFGMINGVRIGLFVSIVVSAATIAVQRQLRTEAPATAPAGRERWTFRRSLTSFN
                     APLRRLLWSDILIRFCERIPFAWVVIYAMDYVGTDAKQVGLLTTVEMLAATLCIIPAS
                     HFADKYGREPFVFVTFIMFTLFPIALWLSGSFAMLALAFAIRGLKEFGDTSRKALIIG
                     YSDPQRRGQVIGTYYLVRDLVVSTGALFGALLWKFGPGVNFVTASGLGAVGTTYYLIT
                     LRRTAGPGVPAPVS"
     gene            1793..2794
                     /locus_tag="VNW28_04610"
     CDS             1793..2794
                     /locus_tag="VNW28_04610"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXA09238.1"
                     /translation="MFYPAGNGRIVLRTEQDWEKDVEVRSVEPEGGISEFLIETERPY
                     FYFKPILLRDGTAQWSRGENFLAVATSGAPLKVHPYFLEDTHCSVCELMPPLASSAGA
                     EHRFRVFLPPGYGENTLKKYPVLYMHDGQNLFFKEEAFLGNTWRADEVLGVLDRMNVI
                     EEVIVVGIYPHERMREYTQPGYEDYGRFLVETLKPLIDAKYRTLSGPKDTAVMGSSLG
                     GVVSFYLGWQWPEVFGKVACLSSTFTFRDNLLERVTAEKKRNIKIYLDSGWPGDNYEA
                     TRSMRDRLLWKGYRPCLELLYLAFPRAKHDENAWAERSPVPLQFLFGKLPVFQGTGA"
BASE COUNT          570 a          925 c          922 g          723 t
ORIGIN      
        1 gaggtctgcg tccacgctcc gccgactcgg gatgacaagc tttttggatt tgtgagatga
       61 gttgtagttg attcgacaaa tgaaatggat cactcgcaaa ggcgtaaaag tggaccgggt
      121 ggcctgcccg tggttgatca gaaaatttgt cgatcccgat gcggagttcg tctttcttcc
      181 acccgagacc gattgggcga gtgtgaaaga cggcacggtt tacgacgtgc cgaattgcga
      241 actcggtcat cgcggcgaag acgtttcctt cgattccatc ctgaagaaat acgagctgac
      301 cgatccggcg ctccgccttc tggcggagat cgtccgcgca gccgattcgc atccctccaa
      361 cccccacccg gccggcgagg gcttgcgctg ggtggctcac ggattcagcg cactcggact
      421 gagcgatcac gaaatcctcg cgcgcgagtt cgtggtttac gatgggctct acgccgagtg
      481 ccagcgccgg gttcgttgat cgaccggtcc ggctgatgaa aaacaggcgc tttctcgcgg
      541 aattcttcgg actgcgccgc aacctcgtca tcctgctcat cgccatcttc gcgatcggcg
      601 cgggagagga gttgtggatg cgcttcatcc cgaaatatct tgaggcgctc ggcgcgacgg
      661 ttttcatcat cggcactttc gatgcgatcc gcaccctgct cggggcgatt tacgcctatc
      721 ccggcggtct ggtggtggat cgctggggat ttcggcacgc tttcatcgcc ttcaaccttc
      781 tttccattgt cggctacgca gtggtgttgc tcattccgca ttggggcgcg gtgctttgtg
      841 cgatgtttct cttcctctca tggacctgct tttcgcttcc ggcaacattc tctctcatcg
      901 ccgaaacact cgacgcgaat cgccactcga tgggaatcgg aatgcagtcg gtgatcaaac
      961 ggctgccgat catgatcgct ccctttctcg gcggcgtgct gatcgatcgc ttcggcatga
     1021 tcaacggcgt gcgaatcggg ctgttcgttt cgatcgtcgt ctcggccgcc acgatcgcgg
     1081 tgcaacgaca actgcgcacc gaggcaccag cgaccgcccc ggcaggccgg gagcggtgga
     1141 cgttccggcg cagcctaacg agtttcaacg cgccgctgcg tcgcctgctc tggagcgaca
     1201 ttctcattcg cttttgcgaa cggatccctt tcgcctgggt ggtgatctac gcgatggact
     1261 atgttggcac ggatgcgaag caggtcggtc tcctgaccac ggtcgagatg ctcgcggcga
     1321 cgttgtgcat catccccgcg tcgcattttg cggataagta tggccgcgaa ccgttcgttt
     1381 tcgtcacctt catcatgttc acgcttttcc cgatcgcgct ctggttgtcg gggagttttg
     1441 ccatgctcgc gctggccttc gcgattcgcg ggctgaagga atttggcgac acctcgcgca
     1501 aggcgctcat catcggctac agcgatccgc agcggcgcgg gcaagtgatc ggcacgtatt
     1561 acctggtgcg cgacctcgtc gtcagcaccg gcgcgctctt cggcgcgttg ctctggaagt
     1621 ttggtccggg cgtcaacttc gtcacggcca gcggcctcgg cgcggtcggt accacttact
     1681 atctcatcac cctccggcgc actgccgggc ccggggtgcc ggcgccggtg tcatgatccc
     1741 gaagttgccc cgactaacaa tattgcaacc aattcgccaa cagatcattc gcgtgttcta
     1801 cccagccggg aatgggagga ttgtgctccg gaccgaacag gattgggaaa aggacgtcga
     1861 ggtccgctcg gttgagccgg agggcggcat ttccgagttc ctgatcgaaa cggagcggcc
     1921 ttacttttat ttcaagccga ttctgctccg cgacgggacc gcccaatggt cgcgcggaga
     1981 aaactttctg gcggtcgcga cttctggcgc gccgctgaag gtccatccct actttttgga
     2041 ggacacgcat tgcagcgttt gcgaattgat gccgccgctc gcgagcagcg ccggcgcgga
     2101 acatcgcttt cgcgtcttcc tgccgccggg ttatggcgag aacacgctta agaaatatcc
     2161 ggtgctctac atgcacgacg ggcagaatct ctttttcaaa gaggaagcgt ttctcggcaa
     2221 cacctggcgg gccgacgaag tgctcggcgt gctcgatcga atgaacgtaa tcgaagaagt
     2281 gatcgtggtg ggaatttacc ctcacgaacg catgcgtgaa tacacccagc ccggttacga
     2341 ggattacgga cgcttcctgg tcgagacact caagcccctg atcgatgcga aatatcgcac
     2401 cctgtccggc cccaaagata ccgcggtgat gggatcctcg cttggcggcg tcgtttcttt
     2461 ttatctcggc tggcaatggc ccgaggtctt cggcaaggtc gcgtgtctct cgagcacgtt
     2521 cactttccgg gacaatctgc tcgagcgcgt gaccgcggag aagaaaagga acatcaagat
     2581 ttacctggat agcggctggc ccggggacaa ctacgaagcc acccgcagca tgcgggatcg
     2641 tttgctctgg aagggatatc gtccctgcct ggagttgctt tacctcgctt tcccccgggc
     2701 gaaacacgac gaaaacgcat gggccgagcg cagcccggtt ccgttgcaat ttctcttcgg
     2761 caagctgccg gtgttccagg gaactggagc ctaggggctc cgttcccctt ttgtgaaatc
     2821 cgcctaattt ggcgaaaaaa gaagctcggg agtggttgat ttctcgaggg tcgaaccccc
     2881 tgcgagacct ttttctgatt ttgggaatct agccggttcg cttcggtctc gttcgagccc
     2941 tacacggtcc ggtactcccg cgactgcggg atcatttgcg gcttttcttt agccgaaatc
     3001 gaaaatcgac gtggtgattt ctcctaggac gggcccttct ccgcgatagc cgcgaatcgg
     3061 cggtgcgcac gatttctccg cgtcccaatc gaacgtgcgc gatccattta atcgaatcaa
     3121 taaagggtaa ataaagttct
//
LOCUS       DATMNS010000127        17430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1614914, whole genome shotgun sequence.
ACCESSION   DATMNS010000127 DATMNS010000000
VERSION     DATMNS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 17430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17430
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1614914"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            518..1441
                     /gene="panE"
                     /locus_tag="VNW28_04615"
     CDS             518..1441
                     /gene="panE"
                     /locus_tag="VNW28_04615"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:NF005094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXA09239.1"
                     /translation="MRILVVGAGAVGGYFGGRLVQAGRDVTFLVHPTRAQQLGRAGLR
                     IISPHGDAVLQPKLVDAEEIDAPYDLVFLSVKAYGLEAATNDFAAAVGPETTILPVLN
                     GMRHVDLLAKRFGEHAVIGGVCLVATEIDEEGRIVQLADFQRLTYGERNGQVTPRVEM
                     LDATLQGAGFDACLSTNIVQAMWEKWVQLASLGAITCLMRGAIGEIVAEPGGADLSLK
                     VVDESVAIATACGHNLPETLLSRHAALMTTPGSPLASSMYRDLRKGAPVEADHILGDF
                     IERGVAHKVAAPLLKAALVNLRVYQGRLPKR"
     gene            complement(1475..1765)
                     /locus_tag="VNW28_04620"
     CDS             complement(1475..1765)
                     /locus_tag="VNW28_04620"
                     /inference="COORDINATES: protein motif:HMM:NF042735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXA09240.1"
                     /translation="MYDKGMVSVEARRNDGSSPEAHGIKGVREKVDWWANKMEVHSFE
                     RSGPFVAHDRSVVRYDIDVTGKDSKKRRRMSEVYAVNDGKIEREEFLPLVKS"
     gene            complement(1858..4083)
                     /locus_tag="VNW28_04625"
     CDS             complement(1858..4083)
                     /locus_tag="VNW28_04625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA09241.1"
                     /translation="MNEASATTPTTPPPRGLQVLILEPDDKVAAEIVSALEEAAPGTS
                     SAVARSLEEAQQLVIDQRPTLFVLDVDATYDLAQEFIYDLRTSHPDARAIILTAIHFA
                     AQREQVAGLGAIHFFEKPFPRGDFVTLVEVLLATGGQAAEGERFQGTLSDLHIADIIQ
                     LKCISGATSMLEFTGPRGEKARVYFENGQVRHATTPTKEGLAAFNEIVDWKGGMISEV
                     PVRHGTPHTINIEWQVLLMDAVRTMDESRSAASEAVANRPPRSSAVDKVLVIDDSLML
                     LSFVKEILEEQNFIVVTAPTAEEGLNICRSSPPDLILLDFVLPDMKGDEVCRRLVADP
                     ATAKIPVVYMSGFGSDLQPIPNVIGSLSKPFTSESLINAVRTYLPGSGDIPDEIKPVV
                     AEEAPPAPPVVPPAPPPIVPVVPSYSLGGNVEPEPPAAPLAEPVPLPIEAAGHAAESG
                     LTDLVPPSLSHQPPSTAPVASVAPSADAKEQPGSSSAYFCGDSSFFSLHWALQTIARQ
                     ELTGTLHAYWARDSVELLARNGRVVVVTTRSPNLYCEEAPVTLLNVERAQIEAARSRQ
                     MTDGCPLFITLANEGLILREPGLQLVQHYGQKLFSQLWTDRVRFTFEQQELPVYSVDL
                     PSSEDHIDQWALSTLRFVQYQELDTKAMPDTSSVPAYTRDGYERVQHLRLTVAEAQFA
                     TQFNGTRSIAQISKNLRLDIKFARLTLSRFLALEIVECWPAQLAQKPESHGGLRGIFG
                     R"
     gene            complement(4486..5886)
                     /locus_tag="VNW28_04630"
     CDS             complement(4486..5886)
                     /locus_tag="VNW28_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HXA09242.1"
                     /translation="MKTGDPFLAAWEQTLARRLDEPALFDTQRRVLARFGEIEEWSRR
                     LETAGGGLQDFKAGEIVAIQIGNHAQWPALFLACLRRGIIALPLERMMSEQERAAALK
                     TCRVAGLMTAPEQPIARLAAVGAVDWGECVPVLLKLTSGTTAAPRAIRFRSAQLFADA
                     VQICDTMGITAADLNFAVIPVSHSYGFSNLLTPLLVRGVPLALSSDRMPRAVLDALAR
                     TESTVFPGMPLFFQAFGEMPETPRLPNLRLCISAGAPLSCAVARRFLENFGRAIHSFY
                     GASECGGICYDHAATGEVEGFVGPALRGVELQALDAGAGATQVRVRSAAVGDGYFPQA
                     EGAKLGAGSFLPDDLVTMSDAGLRLVGRLSDVINVAGKKVNPAEVEAHLLSFAGVRQA
                     VVFGRASLLRNQEVVACVVAAEETDEASLLEFCRARLSGWQVPKRIFLRATIPANERG
                     KISRRELAEYFADRDL"
     gene            5937..6170
                     /gene="rpmE"
                     /locus_tag="VNW28_04635"
     CDS             5937..6170
                     /gene="rpmE"
                     /locus_tag="VNW28_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HXA09243.1"
                     /translation="MKTDIHPDYNETEISCACGAVYHTRSTRRDIKIGICAACHPFFT
                     GEQKFVDTAGRVEKFARRYGSTKATRAGAPKKA"
     gene            6228..7304
                     /gene="prfA"
                     /locus_tag="VNW28_04640"
     CDS             6228..7304
                     /gene="prfA"
                     /locus_tag="VNW28_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXA09244.1"
                     /translation="MDFQSLIERKRERFQQLESEIADPHLFANRKRASDTMRERASIK
                     GLLAKWEELVTARRQLDDNRGLAASPDGDLAQLAQEEIPALEKRVADLEFDVQIALLP
                     PDENDNRDAIVEIRAGTGGSEAAIFAADLYRMYNRYAETAGLKIEDLESSPSELGGLK
                     ETIFKISGESVFRKLRYESGVHRVQRVPATEAQGRIHTSTATVAVLPEAEEVDFELKP
                     EDLRIEVCRAGGPGGQGVNTTDSAVQVMYIPTGRIVRCQDGRSQQKNKEKALSILRAR
                     LLEDKKREEEAKYSATRKSQIGSGGREEKIRTYNFPQNRITDHRIGLTLYNLDRMMEG
                     DLNEMISALQHADLEQRLKESALA"
     gene            7432..8268
                     /gene="prmC"
                     /locus_tag="VNW28_04645"
     CDS             7432..8268
                     /gene="prmC"
                     /locus_tag="VNW28_04645"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HXA09245.1"
                     /translation="MTVLEVLQSTTAYFEKRAIESPRLNAEHLIAHSLGKKRLELYLD
                     FERPLSEPELAPLRELVRRRGQGEPLQHLLGTVEFADHVFVSDKRALIPRPETEQLVE
                     YLCDLAWTENPRILDVGTGSGVIALSLAARFPSAELQAVDLSPDALALARENADRLGI
                     ADRIHFMQGHLLEAIEGTFDLIVANLPYVAASEAAQLAPEVRHDPAVALFGGATGDEL
                     LRELIAAAPPYLRPGGLLALEIGVAQQNALCDFLAAKNYHDICSKKDYAGTPRFLFAR
                     YG"
     gene            8261..9523
                     /gene="murA"
                     /locus_tag="VNW28_04650"
     CDS             8261..9523
                     /gene="murA"
                     /locus_tag="VNW28_04650"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXA09246.1"
                     /translation="MDKILVHGGHPLSGSIKISGSKNSALPIIAATLLTSEPCVLRRV
                     PDLSDTNYMLQILMHLGAEVERASGTVTVKAEKVNSVAPYEVVRKMRASVCVLGPLLG
                     RCKEATVSLPGGCVIGDRPIDLHLKGFEALGAAVRVESGNVKVFAPKLTGAVVNLQGK
                     FGPTVLGTDNVMMAAVLAEGVTVIDGAAAEPEVCDLADFLNKMGAKIDGAGTRRIIIE
                     GVKELHGAEHDIIPDRIETGTFLVAGAICGRGITLNRVRPEHVASVTTALARAGFNVA
                     TTANSISVAPNGAHPRPLELTTQPYPGFPTDMQAQMCALLATTEGTSVITEKIFPQRY
                     MHVAELKRMGAQVEIEGASAVIQGVERLYGAPVMASDLRASAALVLAGLKADGVTEIR
                     RVYHIDRGYEHLDEKLSELGAHIERVKE"
     gene            9528..9905
                     /locus_tag="VNW28_04655"
     CDS             9528..9905
                     /locus_tag="VNW28_04655"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HXA09247.1"
                     /translation="METTPPPLPIPVPSSNTRTWTILCHASALLGLLFHFLGHIFGPL
                     IVWLLKRGDAAEIDAHGKESLNFQLSMLIYDAVAFILCFLLIGIPILIVLWVLNTVLV
                     IVASIKASEGQLYRYPLTIRFIN"
     gene            complement(9958..10836)
                     /locus_tag="VNW28_04660"
     CDS             complement(9958..10836)
                     /locus_tag="VNW28_04660"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HXA09248.1"
                     /translation="MAARAGAARLLLGAHMSIAGGVERAIERARSIECTALQIFVKNN
                     LQWFARPLTRAEIKAFLNHPQRAELGAVFAHANYLVNLAATNPQFHAKSLRALSEELT
                     RADQLRLPFIVLHPGAHLGAGEEAGLGRVVASIDQIFARIPKVKTRLALETTAGQGSS
                     LGHSFEHLAAIIAQVREPERLCVCLDTAHVFAAGYDLTSAAGTRRTFREFDRTVGLRQ
                     LVALHLNDSKTACGSRVDRHEHIGKGQIGLEAFRVIMCDPRFRKIPKVLETPKGKELR
                     EDVENMRMLRALANPP"
     gene            complement(10836..13229)
                     /locus_tag="VNW28_04665"
     CDS             complement(10836..13229)
                     /locus_tag="VNW28_04665"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXA09249.1"
                     /translation="MPSTLLRAPPRPRLHRGRIRHPFGVRVVRLLLRLATVLLLSGLA
                     VGGWYLAKKGFSRNWREKVVEELHKRGVEASVRRLTLDPFRGLIARDVRIYDYKKREK
                     TIAVVSELSLDINYAALFHHQPFLNALDMRNGDLTIPLPPVAGQVSHAELKRFRAHVY
                     FPPERIEVSQAEGLFCGIRISASGQLIKREDYRPATENSAEEAAQRLRLLQSVVTLLQ
                     RFRYPGSAPELQVKFSGDLSRLENARLEATLRGDRIVRGNYEARNFYLTTEWKNLTLS
                     VPQLEWSDQRGRFAGTAAWSRANGQATFQARSSADLKSLLGSFGLGAVLNEVTLFSPP
                     LIEASGSATIGAAEKQFEVVGKVALEKFAYGEIGFEGLTCDFAWDGARTMLRDIRLRH
                     RSGQLNADLLDAPNDFRLNVDSSIVPSALAPLAPPRLRPFLEQWEWARSPNIHLSLKG
                     AGRDPASWRGEGTLALGRARFRGVWMNSASANLRFGDGVLALENLRVTRDEGVGTGAF
                     AYDSVRNEVRLTNVQTTLKPSDAIMWIEPRFWEHVTPYRFHRVPHVTTNGVVQFGGGK
                     QTHLQIDVDAPGGMDYTFIDRVLPFERVAGQLLFTDDRLQILGLKATLFSGGVTGGAD
                     ISLAKGDPHYTAQIAVEKANFPSLTDLYFKYKTSQGALSGRFDFAGRGGDARFLKGEG
                     NVEVTEGNVFAIPIFGPLSELLNKMFAGAGYSVAHQATAPFTIKNGVIHTDKLKVAGN
                     LFSMVGHGDVNFLQNKLDFDIRIDAAGPGAVLTPLYKLFEYHGEGSLTKPIWRPKRF"
     gene            13288..13731
                     /locus_tag="VNW28_04670"
     CDS             13288..13731
                     /locus_tag="VNW28_04670"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HXA09250.1"
                     /translation="MTISDLPATNASLNAISTVLISLGWYFIRRGFWRQHVACMVTAL
                     VSSSLFLTGYIVYHAHVGEKSSGYRGLIAWFYFPMLFSHIALAFVTLPLVMMTLIPVF
                     QRRWDKHRRIARWTMPIWLYVSVTGVLVYLMLYQWFPPASLALGG"
     gene            13773..14216
                     /locus_tag="VNW28_04675"
     CDS             13773..14216
                     /locus_tag="VNW28_04675"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HXA09251.1"
                     /translation="MVTIATYNEPAKAKRLKEKLQQAGVKADLHNEANLQTYAFMSKP
                     QANAKVMVEEPDFARAQALMVEWEAADPDIGAIRCPQCNSPRIEYPQLTRKFLTPALA
                     GILFALKVFPKEFYCQDCHFTWTNQPEHPRYRFWNRFFSGPASEG"
     gene            14315..15148
                     /locus_tag="VNW28_04680"
     CDS             14315..15148
                     /locus_tag="VNW28_04680"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXA09252.1"
                     /translation="MKWIFAFLLAVLIFGGAALFSYKIFIRPEIVMRAERQSTPTPPE
                     DMGLPEFQAAEKLKAAGQLAEARVALTAFIQKYPSGPHVGAAQDLLGEVNISILLSNY
                     PAPEKEEYIVRSGDVLARVATKFKSTPELIMRTNNLSGTMLRIGQKLFISRPEFSILV
                     QRGGKLLYLLDHEHFFKRYALREEKLPANPPAKINTRVAEIMAWRNGKRIGFGSRDFL
                     GSTRWIRLNTPGYIIYSLPDDSHQILDIPPPASGFGMPASDVEELSSLVNPKTAVTIT
                     P"
     gene            15154..16131
                     /locus_tag="VNW28_04685"
     CDS             15154..16131
                     /locus_tag="VNW28_04685"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXA09253.1"
                     /translation="MDSPPLDFEKPILELQRRLQELKSHSDKHEIDFDSEVESMELKI
                     RETRRQIYENLTAWQRVQIARHVRRPFALDYIARCFTDWVELHGDRVFGDDKAMPCGL
                     AKLGPYRCVVISQQKGRDTKENVKRNFGCAYPEGYRKALRVMRLGEKFNLPVIALIDT
                     PGAYPGIGSEERHIAEAIAVNLREMMVLRVPIVATVIGEGGSGGALGIGVADRVLILE
                     NAYYSVISPEACSAILWKDRKHAPEAAEALKLTADDLMRLGVVDEVVSEPEGGAHRDH
                     DLAAANLGAALRRNLKRALETPLDELLRERYAKFRKLGNFAEPKVKNGA"
     gene            complement(16173..17099)
                     /locus_tag="VNW28_04690"
     CDS             complement(16173..17099)
                     /locus_tag="VNW28_04690"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXA09254.1"
                     /translation="MRKCSIRPAAVAKTILLGDTSALMDPKHTLTGDIRPHPQFESRI
                     LGNRRDVLVYLPRGYRRARRRRYPVLYLQDGQNVFDAATSFAGVEWGVDETTQRLLRR
                     NLIEPLIIVAIANTGDDRIHEYTPTPGAIFESALEEKRSRGEAKKYGQFLLEELKPSI
                     DQEYRTKPEAEFTGLGGSSMGGLLTLSLGLSFPDFFHRLMVMSPAVWWDDEVILRVIG
                     RLDYKPPLRIWLDTGTNESGWERARKLRDQLVEKGWRLADDLAYFEAKGADHNEAAWG
                     QRVEAALRFLYPPRSRRRRGRAPQPLLLAAER"
     gene            17139..>17430
                     /locus_tag="VNW28_04695"
     CDS             17139..>17430
                     /locus_tag="VNW28_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09255.1"
                     /translation="MHDRYLRWWTPHLSREFEMLVFGHGGGLPLILYPTSFGNFRQCQ
                     DFDLVDAGAPFVDSGRVTVYCPPIGATCCRITCRRSDQISYATQQDSASNCQK"
BASE COUNT         3411 a         5485 c         5204 g         3330 t
ORIGIN      
        1 tagctggttc ccgtggcgat cgctgtgaga aacgcggccg cgatctgtgc tccacggata
       61 cacggcgcca ctatgatttc aggagcgcca gcctccaagc gccgtattgt tacgtttgca
      121 tgacgagcgt tgctccgtgc ccggtaagtc tcctcgactt aaaaagcgcc tggtcacact
      181 cgcggaatgt tccgattgcg ttacaatggt gtcccagatg agttaaaaca ggagctagcg
      241 ctatcccctg gcttgctctt accggcaaat gatcctctgg aactttttca tgcatcgtga
      301 aacgcggcgg gttagcgcgc atcctcggta ctggccgcgg aggctgggtg aacgaaccta
      361 cctcgtcaac gcttactttg gggcccctgg accgccgatt gtcgagagcc ttgaactcct
      421 ggctggaatt ttcacgcgga acagttttta gagttcacga gggaacgaag agtcggttcg
      481 cgttgaggtt tcattttaga tgcgctttga tatggccatg cgaatacttg tagtcggcgc
      541 cggtgcggtt ggcggatact ttggcggtcg acttgtccag gccgggcgcg acgtgacctt
      601 tctcgtccac ccaacccggg cgcaacagct cggtcgcgct ggccttcgca tcatcagtcc
      661 gcatggtgac gcggtcctac agccgaaatt ggtcgacgcg gaggagatcg acgcacctta
      721 cgatctcgtt ttcctcagcg tgaaggctta tgggctggag gcggcgacga acgatttcgc
      781 cgcggccgtc ggaccggaaa caaccatcct gccggtgctt aacgggatgc gtcacgtcga
      841 tctcctagcg aaacgtttcg gagaacatgc agtcatcggc ggcgtctgtc tcgtcgcgac
      901 cgagatcgac gaggagggtc gcatcgtcca gctcgccgac tttcagcgac ttacttatgg
      961 cgagagaaac ggtcaggtaa cgccccgggt agaaatgctc gacgcgacgc tgcagggcgc
     1021 gggattcgac gcctgtctgt cgaccaatat cgtgcaagcg atgtgggaga aatgggtcca
     1081 gctcgccagc cttggcgcca tcacctgttt gatgcgcggg gcgatcggcg agatcgtggc
     1141 cgaacccggc ggagccgacc tctcactcaa ggtagtggac gaatcagtcg ccattgccac
     1201 ggcctgcggc cacaatctgc cggaaacatt actctcacga catgccgccc tgatgaccac
     1261 gccgggatct ccgctcgcgt cctcgatgta tcgcgatctg cgcaaaggcg cgccggtcga
     1321 ggccgatcat atcctgggcg atttcatcga gcgcggcgtc gcgcacaaag tcgccgcgcc
     1381 attgttgaag gccgctttgg ttaatctccg ggtctatcag ggcaggctcc cgaagcgttg
     1441 agatgcgcgg ctgtaatccg ccacgacgcc cgcctcaact cttcacgagt ggcagaaatt
     1501 cttcccgctc gatcttgcca tcgttgaccg catagacctc cgacattcgc ctccgcttct
     1561 tggaatcctt gccggtgaca tcgatgtcgt atcgcacgac agaccggtcg tgcgcgacaa
     1621 aaggaccact cctttcaaaa ctgtgtacct ccatcttgtt cgcccaccaa tcgaccttct
     1681 cgcgcacgcc tttgatgccg tgtgcttccg gcgaactgcc gtcattcctc cgcgcttcca
     1741 cgctcaccat gcccttgtca tacaaggtct ggaccgctgc agtttcccta acgaccgcgt
     1801 ttgtcagaag agccaggata agatgccgtc cgtcgcggca cacctggagc tcgctaccta
     1861 acgaccgaaa attccacgca acccaccgtg cgactccggc ttttgcgcga gctgcgccgg
     1921 ccagcattcc acgatctcga gcgcaaggaa gcgggagagg gtcaggcgcg cgaacttgat
     1981 gtcgagccgc aaattcttgg agatctgcgc gatcgatcga gtgccgttaa attgcgtggc
     2041 aaattgcgcc tccgcgacgg tcaggcgcaa atgctgcacg cgctcatagc cgtcacgggt
     2101 gtaagccggc accgacgacg tatccggcat cgccttcgtg tccagctcct gatactggac
     2161 aaaacgcagg gtactcagtg cccactgatc aatgtgatcc tcggacgacg gcagatcgac
     2221 ggaataaact ggcaactcct gttgctcgaa ggtgaaccgg acgcgatccg tccagagttg
     2281 cgagaacaat ttctgcccgt agtgctgcac gagctgcaaa cccggctcgc gcaagatcag
     2341 cccctcgttc gcgagggtga taaataaggg acagccatcc gtcatttggc gcgagcgcgc
     2401 tgcttcaatc tgcgcgcgct cgacgttcaa caaagtgacg ggcgcctctt cgcaatagag
     2461 gttcgggctg cgcgtcgtaa caaccacgac ccgaccgttg cgcgccagca gttcgaccga
     2521 gtcccgcgcc caatacgcat ggagggttcc ggttaactcc tggcgggcaa tcgtctgcag
     2581 agcccagtgc agggaaaaga aactggagtc gccgcagaaa taggcgctgc tggacccggg
     2641 ctgctctttc gcatcagcgg agggcgcaac cgacgcgacc ggtgccgtgg aaggcggttg
     2701 gtggctcaaa ctgggcggaa ccagatccgt gagcccggac tccgcggcgt ggccagcggc
     2761 ttcgattggc aggggcaccg gctcggccag cggagctgcc ggcggctccg gttccacgtt
     2821 tccgcctaat gaataggacg ggaccaccgg cacgattggc ggcggcgccg gcggaacaac
     2881 gggcggggcc ggcggcgcct cctccgctac aaccggcttt atttcgtcag ggatgtcccc
     2941 actgcccgga agataagtcc gcaccgcgtt gatgagcgat tccgaggtga acggcttgct
     3001 taaagaaccg atcacattcg ggatgggttg gagatcgctc ccaaatcccg acatgtaaac
     3061 caccggaatt ttggccgtgg ccggatcggc cacgaggcga cgacacactt cgtcgccctt
     3121 catgtcgggc aagacgaaat cgagcaggat caagtcgggc ggggaagagc gacagatgtt
     3181 caaaccctct tccgcggtcg gcgctgtcac gacgataaaa ttttgttctt ccaaaatttc
     3241 cttcacaaaa ctgagcagca tcaggctgtc gtcgattacc aaaaccttat cgacggccga
     3301 ggatcgcggc gggcggttgg ccacggcttc cgatgccgcg ctgcgcgatt cgtccatcgt
     3361 gcgcactgca tccatgagca gcacctgcca ttcgatattg atcgtatgcg gcgtcccgtg
     3421 ccggaccggc acttcggaaa tcatgccacc tttccagtcc acgatttcgt taaacgccgc
     3481 cagcccttcc ttggttggcg tagtcgcgtg acgcacctgc ccattctcaa aataaacccg
     3541 cgccttctcg ccgcggggcc cggtgaactc cagcatcgaa gtcgccccac tgatgcactt
     3601 cagctggatg atatcggcga tgtgcagatc gctcagcgtc ccttgaaaac gctcgccctc
     3661 ggccgcctgg ccgccggttg cgaggagcac ctcgaccaaa gtcacaaagt cgccccgcgg
     3721 gaatggcttt tcgaaaaaat gaatggcacc caaaccggca acctgctcgc gctgcgcggc
     3781 gaaatgaatc gccgtcagga tgatcgcgcg ggcatcggga tggctcgtgc gcagatcgta
     3841 gatgaactcc tgcgcaaggt cataggtcgc gtccacatcg aggacgaaaa gtgtcggcct
     3901 ttggtcgatg acgagttgct gcgcttcctc tagcgagcgg gcgacggccg agctcgtccc
     3961 gggcgctgcc tcttcgaggg cggaaacgat ttccgcggcg accttgtcat ctggttcgag
     4021 gatcaggacc tgcaggccgc ggggtggtgg ggtggtggga gtggtggcgc ttgcttcgtt
     4081 cacagtggtc gacagacagc aagtgacgaa ccaatccttc gactcttacc gctgtctcga
     4141 cctagaaatg cctgctgaac gcgaaatgga aagcattttt ttccgcaaaa tggcgccgct
     4201 ccctcgccac gcacagatag gctgtgctca agtttcgtag cggccccgcc agcggcgcgt
     4261 tccaaccgct gccgggatac gcgcccgagc taattggtgt gcacgccagt cgtcaccgca
     4321 tgcccgttgt cggtgcgaac tgtcgtggaa cagctggaaa aagccgccag caggatgatt
     4381 gcgccgagtg tggaaagaag aatggttcgc ataatgatgt gtttggtttc cttacgattc
     4441 gtaaacaacc ccccgcgcgg gtgtctggcc acgcgcattt gttcgtcata aatctcgatc
     4501 cgcgaaatat tccgccagct cgcggcggct gatcttcccg cgttcgttgg cggggatcgt
     4561 ggcgcgcaag aaaatccgct tcggcacctg ccaaccgctg agacgcgcgc gacaaaattc
     4621 cagaagcgac gcttcgtccg tttcctccgc cgcgacgaca caggcgacca cttcctggtt
     4681 gcgcaacaac gacgccctgc caaaaacgac tgcctgccgg acacctgcga aactcaggag
     4741 atgcgcctcg acctcggccg gattgacctt ttttcccgcc acgttaatca catccgagag
     4801 ccgcccaacg aggcgaagac ccgcgtcgct catcgtcacg agatcgtccg gcaggaacga
     4861 cccggcgccg agtttggccc cttccgcctg cggaaaatag ccatcgccca cggccgcgct
     4921 cctgacccga acctgggtcg cgccggcgcc ggcatcgagc gcttgcagct caaccccgcg
     4981 cagcgccggc ccgacaaaac cttccacttc acccgtcgcg gcgtgatcat agcaaatgcc
     5041 gccgcactcc gaggcgccat agaacgaatg aattgctcgg ccaaaattct ccaaaaacct
     5101 gcgcgcgaca gcacaactca gcggcgcgcc cgcggaaatg cacaaacgca gattcggcag
     5161 ccgcggcgtc tccggcattt cgccgaacgc ctggaaaaaa agtggcatcc ctggaaacac
     5221 cgtggactcg gtccgtgcca gagcgtcgag caccgcgcgc ggcatccgat cgctgctcag
     5281 cgccagcggc acaccgcgaa ccaacaacgg tgtcagcagg ttgctgaagc cgtaggaatg
     5341 cgacaccggg atgaccgcga agttcaagtc cgcggcggtg attcccatcg tgtcgcaaat
     5401 ctgcaccgca tctgcgaaca gttgcgcact gcggaaacga atcgcccgtg gcgccgcggt
     5461 cgtcccggaa gtgagcttga gcaagaccgg aacgcactcg ccccaatcga ccgcgccgac
     5521 cgcagcgagc cgcgcgatcg gctgctccgg agcggtcatc agcccagcga cgcggcaggt
     5581 ctttaaggcc gcggcgcgtt cctgctcgct catcatccgc tcgaggggca gcgcgatgat
     5641 gccacgccgc agacaagcga ggaagagtgc cggccactgc gcgtgatttc caatttggat
     5701 cgcaacgatc tcgccagctt tgaaatcctg caaaccgccg cccgccgttt cgaggcgccg
     5761 gctccattcc tcgatctctc cgaagcgggc gagcaccctg cgctgcgtgt cgaatagcgc
     5821 cggctcatcc agccgccgcg ccagcgtctg ctcccatgcc gcgaggaagg gatcgccggt
     5881 tttcatcact tgcgacccgc cccttccgcg actactgtgc ccactctttt tccactatga
     5941 aaacggacat tcatcctgac tacaacgaga cggaaattag ctgcgcttgc ggcgcggtct
     6001 atcatacccg ctcgacgcgc cgggacatta aaatcggcat ctgcgccgcc tgccatccgt
     6061 ttttcaccgg cgaacagaag ttcgtggaca ctgcgggccg ggtcgagaaa ttcgcccgtc
     6121 gctacggcag cacgaaggca acccgcgccg gcgcccccaa aaaagcctag ctgatttcgc
     6181 ctaacgaacg gtgattgcgc cccggcgtgc agtcgccgtt tttgtttatg gactttcaat
     6241 ccctcatcga gcgcaaacgc gaacgcttcc agcaactgga aagcgagatc gccgatccgc
     6301 accttttcgc taatcggaag cgggccagcg acacgatgcg cgagcgcgcg tcgatcaagg
     6361 gtttgctcgc gaaatgggag gaactggtga ccgcgcggcg gcagttggat gacaaccgcg
     6421 ggctggccgc gtcgcccgat ggcgacctcg cacaactggc gcaggaagaa atcccggcgc
     6481 tcgaaaagcg cgtcgccgat ctcgagttcg atgtgcagat cgcgctcctg ccgccggatg
     6541 aaaacgataa ccgcgacgcg atcgtggaga ttcgcgccgg caccggcggg agcgaagccg
     6601 ccatcttcgc ggccgatctt taccgcatgt acaaccgtta tgcggaaacg gccgggctaa
     6661 aaatcgaaga cctcgagtcg agcccgtccg agctcggcgg tttgaaggaa accatcttca
     6721 agatttccgg cgaatcggtt ttccgcaagc tgcgttacga gagcggggtc catcgggtgc
     6781 aacgcgtgcc ggcgaccgaa gcccagggcc gcatccacac ctctaccgcg accgtcgccg
     6841 tcctgcccga agccgaggag gtcgacttcg agctcaaacc ggaagatctc cggatcgaag
     6901 tctgccgcgc cggcgggccc ggtggccagg gtgtgaacac gaccgattcc gcggtccagg
     6961 tgatgtatat tccgaccggc cgcatcgtgc gctgccagga tggccgcagc cagcagaaga
     7021 acaaggaaaa ggcgctcagt atcctgcgtg cgcgcctgct cgaggataaa aaacgggagg
     7081 aggaagcgaa gtattccgcc actcgcaaaa gccagatcgg ttccggcgga cgcgaagaaa
     7141 aaatccgcac ctacaacttc ccgcaaaacc ggatcaccga ccaccggatt gggcttacgc
     7201 tctataatct cgaccgcatg atggagggcg atctgaatga aatgatctcg gccctgcagc
     7261 acgccgacct cgaacagcgg ctgaaggaat cggccctggc ctaacgagtg gagacgcctg
     7321 gcagacgcct ctctgcggga ggcgcgagat ttggggataa atgcaaaact ccacggcgcg
     7381 cggatctgcc ccgcactgcg cagagcacag cgccggccgg cgcgtgaggc aatgaccgtc
     7441 ctcgaagtcc tgcaatcgac gaccgcctac tttgagaagc gcgcgatcga gagcccgcgc
     7501 ctcaatgccg agcatctcat cgctcattcg ttaggcaaga agcgcctcga gctttacctc
     7561 gacttcgagc gcccgctctc cgagccggaa ctcgcacccc tgcgtgaact cgtcaggcgg
     7621 cgcggccagg gcgagccgct ccaacatctt ctggggacgg tcgaattcgc cgaccatgtc
     7681 tttgtcagcg acaaacgtgc tctcatcccg cgccccgaaa ccgagcaact cgtcgaatat
     7741 ctgtgcgacc tcgcctggac ggagaatccg cgcatcctcg atgtcggaac tggcagcggg
     7801 gttatcgcgc tctcgctcgc cgcccgtttt ccctccgcgg aattgcaggc ggtcgatctc
     7861 tccccggatg ccctcgccct ggctcgcgaa aatgcagacc gcctcgggat tgccgaccga
     7921 atccatttca tgcaaggcca tctactcgaa gcgatcgagg gcaccttcga tttaatcgtc
     7981 gccaaccttc cttatgtcgc ggcgagcgaa gcggcgcagc tcgcgcccga agtgcgccac
     8041 gatccggccg ttgcactgtt tggcggagcg actggcgacg aactgctccg ggaactcatc
     8101 gccgctgcgc cgccctattt gcggccgggc ggcctcctcg cgctcgagat tggtgtcgct
     8161 caacaaaacg cgctctgcga ctttctcgcg gcaaaaaatt accacgacat ttgctcgaaa
     8221 aaggactatg caggaacccc acgtttcctc ttcgcgcggt atggataaga ttcttgttca
     8281 cggcggtcat ccgctctcgg ggtcgatcaa gatcagcggc tccaagaact cggccctgcc
     8341 gatcatcgcc gcgactttgc tcacgtccga gccgtgcgtc ctgcgtcgtg tcccggacct
     8401 gagcgacacg aattacatgc tccagatcct gatgcatctc ggcgccgaag tggagcgggc
     8461 cagcgggacg gtgacggtca aggcggagaa ggtaaactcg gttgcgccct atgaagtggt
     8521 gcgcaagatg cgggcctcgg tctgcgtcct cggtccattg ttaggccggt gcaaggaggc
     8581 gaccgtctcg ctccccggcg gttgcgtcat cggcgatcgc ccgatcgatc tgcatctcaa
     8641 gggttttgaa gcgctcggcg cggcggtgcg ggtggaaagc ggcaacgtca aagtgtttgc
     8701 gcccaagctc accggcgccg tggttaatct gcagggcaaa tttggaccga ccgttctcgg
     8761 cacggacaac gtcatgatgg cggccgtgct ggcggaaggc gtgaccgtga tcgacggcgc
     8821 ggcggccgaa ccggaagtgt gcgacctggc ggatttcctg aacaagatgg gcgcgaaaat
     8881 cgacggcgca ggcacgcgcc gcatcattat tgaaggcgtg aaagagctgc acggcgccga
     8941 gcacgacatc atcccagacc gcatcgagac cggcacattc ctggtcgcgg gcgcgatttg
     9001 cggccgcggg atcaccctga atcgggtccg gcccgagcac gtcgcgtcgg tgaccacggc
     9061 cctggcgcga gcgggcttca acgtggcgac gaccgcgaac tcgatttcgg tcgcgccgaa
     9121 cggcgcccac cccagaccgc tggaactaac cacccagccg taccccggtt tcccgaccga
     9181 catgcaggcg caaatgtgcg cgctccttgc cacgacggaa gggaccagcg tgatcaccga
     9241 aaaaattttc ccgcagcgtt acatgcacgt tgcggagttg aagcggatgg gcgcacaggt
     9301 cgagatcgaa ggcgcgagcg cggtcatcca gggagtcgag cgcctctacg gtgccccggt
     9361 catggcgagc gatctgcgcg cttccgcggc gctcgtcctg gcgggtttga aagcggatgg
     9421 cgtaaccgag attcgccgcg tctatcacat cgaccgtggc tacgaacatc tcgatgaaaa
     9481 actgagcgaa cttggcgcgc acatcgaacg tgtgaaagaa taacgacatg gaaacgacac
     9541 caccaccact gccgattccg gtcccgtcct ccaacactcg cacctggact atcctctgcc
     9601 atgccagcgc gctgctgggc ctcctttttc attttctcgg ccatatcttt gggccactca
     9661 tcgtctggct tctcaaacgc ggcgacgcgg cggaaatcga cgcccacggc aaagagtcac
     9721 tcaactttca gctttcgatg ctgatctacg acgccgtcgc cttcatcctt tgcttcctcc
     9781 tgatcgggat tccgattctg attgttctct gggtgctcaa tacggttctg gtcatcgtcg
     9841 cctcgatcaa agcgagcgaa gggcagcttt atcgttatcc cctaacgatt cggtttatca
     9901 attagggagc gatgacgctt tgcctgtctt ctcctcgcgc ttcgcgaagt gaagcgtcta
     9961 cggcgggttc gcgagcgcgc gcagcattcg catgttttcc acgtcttccc gcagctcctt
    10021 gcctttcgga gtttccagga ccttcgggat tttgcggaaa cggggatcac acatgatgac
    10081 gcggaacgcc tccaggccta tctgaccttt cccgatatgc tcgtgacgat ccacccgcga
    10141 tccgcacgcc gtcttcgagt cgttcaagtg caaggctacg agctgtcgca acccaaccgt
    10201 ccggtcgaat tcgcggaatg ttcttcgcgt tcccgccgca ctcgtcaggt cataaccggc
    10261 cgcgaagacg tgcgcggtat cgaggcaaac gcagagccgc tccggctcgc gcacctgcgc
    10321 gatgatggca gccaggtgct cgaaactgtg accgagagac gagccctggc ctgcggtcgt
    10381 ctcgagcgca agccttgttt tcacctttgg aatccgggca aaaatttgat cgatcgacgc
    10441 caccacccgc ccgagacccg cttcttcgcc ggctccgaga tgcgcgcccg gatgaaggac
    10501 aatgaagggc aggcgcagct gatcggcacg ggttaattcc tcggagaggg cgcggaggga
    10561 cttggcgtga aactgcgggt tggtcgcggc gagattgacc aggtaattgg cgtgcgcgaa
    10621 gactgcgccg agctcggccc gctgcggatg gttcaggaac gccttgattt ccgcgcgggt
    10681 caacggccgg gcaaaccact gcaggttgtt cttcacaaaa atctgcagcg cggtgcactc
    10741 gatcgagcgc gcgcgctcaa tcgcgcgctc caccccgccc gcgatcgaca tgtgcgcgcc
    10801 taacaaaagg cgcgcggctc ccgcccgggc cgccatcaga accgtttcgg ccgccagatt
    10861 ggtttcgtta ggctgccttc gccgtgatat tcgaagagtt tatagagcgg cgtcaggacc
    10921 gcgcccgggc cggccgcgtc gatccggata tcgaagtcga gtttgttctg gaggaaatta
    10981 acgtcgccgt gcccgaccat ggagaacagg ttgcccgcga ccttcagctt gtcggtgtga
    11041 atgacgccat tcttgatcgt gaaaggcgcg gtcgcctggt gcgcgacgct gtagcccgcg
    11101 ccggcgaaca tcttgttcag cagttcggag agcggcccga aaatcggaat cgcgaaaacg
    11161 ttgccctcgg tgacctcgac gtttccctcc cctttgagga aacgcgcgtc tcctcctcgg
    11221 ccggcgaaat cgaaccggcc actgagcgcg ccctgcgagg tcttatattt gaaatagaga
    11281 tccgtcagac tgggaaaatt tgccttttcg acggcgatct gcgcggtgta atgcgggtcg
    11341 cctttggcta acgaaatgtc ggcgccgccg gtgacaccgc cggagaatag agtcgccttc
    11401 aggccgagga tttgcagacg gtcgtcggtg aaaagcaact gcccggcgac ccgctcgaag
    11461 ggcagaaccc ggtcgatgaa cgtgtagtcc atcccgccgg gggcatcgac atcgatctgc
    11521 aaatgagtct gtttcccgcc gccgaactgc accacgccgt tcgtcgtaac gtggggtacg
    11581 cgatggaagc gataaggcgt gacgtgttcc cagaagcgcg gctcgatcca catgatcgca
    11641 tcgctcggtt tcagggtggt ttgtacgttc gttaggcgga cctcgttgcg aaccgaatcg
    11701 taggcgaagg cgccggttcc gacgccctcg tcgcgcgtca cgcgcaggtt ttcgagcgcc
    11761 agaacgccgt cgccaaagcg caggttggcg ctggcgctgt tcatccagac cccgcgaaac
    11821 cgcgcgcggc cgagcgcaag ggtgccctcg ccgcgccaac tcgcgggatc tcgccccgcc
    11881 cccttgaggc tgaggtgaat attgggcgaa cgcgcccatt cccattgctc gagaaacggg
    11941 cggagccggg gcggcgcgag cggggccagc gcgctcggga cgatcgaact gtcgacgttc
    12001 aatcgaaagt cgttaggcgc atcgagcaga tcggcgttga gctgcccgct gcgatgccgc
    12061 aggcggatat cgcgcaacat cgtgcgcgct ccgtcccacg cgaaatcgca ggtcaacccc
    12121 tcgaaaccga tctccccgta ggcaaacttt tcgagcgcga ctttgcccac cacttcgaat
    12181 tgtttttcgg ccgccccgat ggtcgcagag ccggacgcct caatcagcgg cggcgaaaaa
    12241 agggtcacct cgttgagcac ggcgccgagg ccaaagctgc ctaacaatga tttgaggtcc
    12301 gcgctgctgc gcgcctgaaa ggtcgcctgt ccgttggccc gactccatgc cgccgtgccg
    12361 gcaaagcgac cccgctggtc gctccactcc agctgcggaa cgctcagcgt caggtttttc
    12421 cactcggtcg tgagataaaa atttcgcgct tcgtaattgc cgcgcacgat tcgatcgccc
    12481 cgcagcgtgg cctcgagccg cgcattttcc agccgggaaa gatcgccgga aaatttgacc
    12541 tgcaattccg gcgcgctgcc gggataacga aaacgctgca aaagagtgac cacgctctgg
    12601 aggaggcgga gccgctgcgc ggcttcctcg gccgagttct ccgtcgccgg ccgatagtct
    12661 tcgcgcttga tcaactgccc gctggccgag atgcgaatcc cgcagaaaag accctccgcc
    12721 tggctcactt cgattcgctc cggcggaaaa taaacgtggg cgcgaaatcg tttcagctcg
    12781 gcatgggaga cctgcccggc aaccggcggg agcggaatcg tcaggtcacc gttgcgcatg
    12841 tcgagcgcgt tgaggaacgg ctggtgatga aaaagcgccg catagttgat atcgagcgaa
    12901 agctcgctca ccaccgcgat cgttttctcg cgtttcttgt agtcgtaaat gcgcacgtcg
    12961 cgcgcgatca atccgcggaa gggatccagc gtcaggcggc ggaccgacgc ttcgacaccg
    13021 cgtttgtgca gttcctcgac gactttttcg cgccaattgc gactgaaacc ttttttcgcc
    13081 agataccagc caccgaccgc gagtccgctg aggagcagga cggtcgccag gcgcaggagc
    13141 aggcggacga cccgcacgcc aaacggatgg cggatgcgcc cgcgatgcag tcgcggccgg
    13201 ggcggcgcgc gcagcagggt cgaaggcatg cctgactcct acgagcggcg gttgttattt
    13261 caagccgggc gcgcgtaggc tccgggcatg acgatctcgg acttgccggc gacgaacgcg
    13321 tcgctcaatg cgatcagcac ggttttgatt tcgttaggct ggtattttat ccggcgcggg
    13381 ttctggcggc aacacgtcgc ctgcatggtc accgcgctgg tttcgtcgtc cctttttctt
    13441 accgggtaca ttgtctatca tgcgcacgtc ggcgaaaagt cgtcgggcta tcgcggattg
    13501 atcgcctggt tttattttcc gatgctcttc tcgcatatcg cgctcgcctt cgtcaccctg
    13561 cccctggtga tgatgaccct gatcccggtt ttccagcggc gctgggacaa acatcgccgg
    13621 atcgcgcgct ggacgatgcc gatttggctt tacgtttcgg tgaccggcgt gctggtttac
    13681 ctgatgctct accaatggtt cccgccggcc agtcttgcgc tcgggggtta gttgatgttt
    13741 gatcagacac gcccgacgac tgcgggcatt ttatggtgac gatagcgacc tacaacgagc
    13801 cggccaaagc caaacggctg aaggaaaaac tccagcaggc cggagtgaaa gccgacctgc
    13861 ataacgaggc caatttgcag acctacgctt tcatgtccaa gccgcaggcg aacgcgaaag
    13921 tgatggtcga ggagccggat ttcgcgcgcg cccaggccct gatggtggaa tgggaggcgg
    13981 ccgatcccga catcggcgcg atccgctgtc cgcagtgcaa ttcaccgcgg atcgagtacc
    14041 cgcagctgac ccgcaaattt ctcaccccgg cgctggccgg aattcttttc gcgctgaaag
    14101 ttttcccgaa ggaattctac tgccaggatt gccatttcac ctggacgaac cagccggaac
    14161 atccgcgcta tcggttctgg aaccgcttct tttccggccc ggcaagcgag ggttgaggcg
    14221 gtcacaatgg cctcgttagg gcgacaaatc agccggtcgc aatgatcgtc gccgacgccg
    14281 ccgaacgagg gtcgcgcagc gcgtcgatcg ctcgatgaaa tggattttcg ctttcctgct
    14341 cgcggtcctg atttttggcg gggccgccct ttttagctac aagatcttta ttcgcccgga
    14401 gatcgtgatg cgggccgagc gccaatccac gccgacgccg ccggaggata tgggcctgcc
    14461 cgaattccag gccgcggaaa aactgaaggc ggcgggccaa ctggccgaag cgcgcgtggc
    14521 cttgaccgca tttattcaga agtatccaag cggcccccac gtcggcgccg cgcaggacct
    14581 gcttggcgaa gtgaacatca gcatcctgct ctcgaattat ccggcgccgg aaaaggagga
    14641 atacatcgtg cgcagcgggg acgtgctggc gcgggtcgcg acgaagttca agtcgacccc
    14701 cgagttgatc atgcgcacga ataacctcag tggaacaatg ttgcgcatcg gccaaaagct
    14761 gttcatttcg cgtccggaat tttccattct cgtccagcgc ggcggcaaac tgctctacct
    14821 tctcgaccac gagcatttct tcaaacgcta cgccttgcgg gaggaaaaat tgcccgcgaa
    14881 tccgcccgcg aaaatcaaca cccgggtcgc ggaaatcatg gcctggagga atggcaaacg
    14941 gatcggcttc gggagccggg atttcctcgg cagcacccgc tggatccgcc tcaacacgcc
    15001 cggctacatc atctattcgc tgccggacga ttcccaccaa atactcgaca ttccgccgcc
    15061 cgcgtcgggc ttcggcatgc cggcctccga cgtggaggaa ttgagcagcc ttgtaaaccc
    15121 gaaaacggct gtcaccatta ctccctagcg accatggatt cgccaccgct cgatttcgaa
    15181 aagccgatcc tggaattgca gcgccgcctc caggaactca aatcgcattc cgacaagcac
    15241 gagatcgatt tcgactcgga ggtggagtcg atggaattga agatccgcga gacgcgccgt
    15301 cagatttacg agaatctgac cgcctggcag cgggtgcaga ttgcgcgcca tgtgcgccgg
    15361 ccgttcgcgc tcgattacat tgcccgctgc tttaccgact gggtggaatt gcacggcgat
    15421 cgcgtcttcg gcgatgataa ggcaatgccc tgcggcctgg ccaagctcgg gccctaccgc
    15481 tgtgtcgtga tttcgcaaca gaagggccgc gacacgaagg aaaatgtgaa gcgcaatttt
    15541 ggttgcgctt acccggaagg ctatcgcaag gcgctccgcg tgatgcgcct cggtgagaaa
    15601 ttcaatctgc cggtgattgc gttgatcgac accccaggcg catatcccgg catcggctcc
    15661 gaggaacgcc acatcgcgga agcgatcgcg gttaatctgc gcgaaatgat ggtcttgcgc
    15721 gtgccgattg ttgcgaccgt catcggcgaa ggcggctcgg gcggcgcgct cgggatcggg
    15781 gtcgcggatc gcgtcctcat cctcgagaac gcctactatt cggtcatcag tccagaagcc
    15841 tgctcggcga ttttgtggaa ggatcgcaag cacgcgcccg aggcggcgga ggcactcaag
    15901 ctgacggcgg acgatttgat gcgactcggc gtggtcgacg aagttgtctc ggaaccggaa
    15961 ggaggtgcgc atcgcgatca cgatctggcg gcggcgaacc tgggcgcagc tctgcggcgc
    16021 aatctgaaac gcgcgctcga gacgccgctc gatgagctgc tgcgggagcg ctacgcaaaa
    16081 ttccgcaagc tcggcaactt cgccgagccg aaggtcaaga acggagcctg acgcctaacg
    16141 aaattttcgg cgcaagccgc cctctatccc gcctaacgtt ccgcggcgag caggagtggc
    16201 tgcggcgccc gccccctcct ccggcgcgac cgtggcggat acagaaaacg cagcgctgct
    16261 tcgacgcgct gcccccaggc cgcttcgttg tggtcggcgc ccttcgcttc gaaataagcg
    16321 agatcatcgg ccaggcgcca gcctttctcg accagctggt cgcgcagttt gcgggcgcgt
    16381 tcccagccgc tttcgttggt tcccgtgtcc agccagatcc tgagcggagg tttataatcg
    16441 agtctcccga tcacgcgcag gatgacttcg tcatcccacc acacggccgg tgacatgacc
    16501 atgagccggt ggaaaaaatc cgggaacgag agcccaagcg acaacgtcag caggccaccc
    16561 atggacgatc ctcccagacc ggtaaattcc gcttcgggtt tcgtgcggta ttcctggtcg
    16621 atggagggct tgagctcttc cagcagaaac tggccgtatt tcttcgcctc gccccggctg
    16681 cgtttttctt ccagggcact ttcgaaaatc gcgccgggag tcggggtata ttcgtgaatt
    16741 cgatcgtcgc ccgtattggc gatcgcgacg atgatgagcg gctcgatcag attccggcga
    16801 agcaaacgct gcgtcgtttc gtccaccccc cactccaccc cggcaaagga ggtagccgcg
    16861 tcaaaaacat tctggccgtc ctgcaaataa agcaccggat aacgccgtcg ccgcgcgcgc
    16921 cggtaaccgc gcggcagata aaccagcacg tcgcggcggt tgcccagaat gcgcgattcg
    16981 aattgcggat gcggtcggat atcgccggtc aacgtgtgtt tcggatccat cagcgcgctt
    17041 gtatcgccga ggagaatcgt cttggcaacc gccgcgggtc ggatgctgca ttttctcatc
    17101 gccaggtgcg tcgccgtcgt cttaattcgg agtcacgaat gcatgaccgt tatctccgct
    17161 ggtggacgcc gcacttgagt cgcgaattcg agatgcttgt cttcggccac ggtggcgggt
    17221 tgccgctcat cctttatccg acctccttcg gcaattttcg ccagtgccag gatttcgacc
    17281 tggtcgacgc cggcgcgccg ttcgtcgaca gcggccgcgt gaccgtttac tgccccccta
    17341 ttggcgcgac atgctgccgt attacttgtc gacgttctga ccagattagt tatgcgaccc
    17401 agcaagattc ggcgagcaat tgccaaaaga
//
LOCUS       DATMNS010000128         9383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4839745, whole genome shotgun sequence.
ACCESSION   DATMNS010000128 DATMNS010000000
VERSION     DATMNS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4839745"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VNW28_04700"
     CDS             <1..325
                     /locus_tag="VNW28_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09256.1"
                     /translation="PFRKENKMKPNDQIESVKWLSRYDDPTRRGGSLPRITFEYQQRR
                     GGGSTFGGASPARQPGFTRLANEVLRSDASRSFRLETAVLGFVTLVSAWPIAVMIHEV
                     IRLLQ"
     gene            complement(394..3423)
                     /gene="pruA"
                     /locus_tag="VNW28_04705"
     CDS             complement(394..3423)
                     /gene="pruA"
                     /locus_tag="VNW28_04705"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF002852.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HXA09257.1"
                     /translation="MSALQAATERRGQRIFELVDQYPESIFSKAGFYQKMMAFSMRDE
                     TFKVQMFRFVDVLASLRRSSDIVVHLREYFHGMDGVVPMLQTGLRAAGIFPWLTAFVL
                     RRNVSGMARQFIAGKDGADVMKTLRRKRKEKIGFTVDLLGEAVVSETEADEYAARAME
                     LLETLSRETRGWTDPLGKNAELFPVVNLSLKISAFYSQMDPAAPEEAVAHLAPKLRPI
                     LRRAKEVGAFVNFDMESYAQKNGTLDLFKQLFTEPEFADWPHVGIVVQAYLRDGEPDL
                     RDLIEWGRRRGTRFAVRLVKGAYWDYEKIVAQQNGWVCPVFLQKPESDASFETCTRIL
                     LENESVVTAAFGSHNVRSIAHAMACAEELGIDQSRFEFQLLYGMAGPIKRALVEMGHR
                     VREYSPVGELLPGMSYLVRRLLENTSNEGFLRAKFSDHQSAAQLLRDPKELITTEEPT
                     KVQPNQEPSADGAAYENAPAVNFVHRQTQEQMKTALRAARSQFGKRYPLVIGGERVTT
                     GKWMASVNPSAPNEIVGEVAEAGVPEAERAVKAASAAFEHWCRVSIEHRAQLLERVAA
                     IMARRRLELAAVEVYEVGKAWEEADGDIREAVDFCRFYAQQMRGMGRPKLTQHVLGEE
                     SYQHYWPRGVALVIAPWNFPIAILTGMVSAAIVTGNTVIMKPAEPSSVTGALLMEMFE
                     EAGVPPGVLNYLPGHGSVVGQHLVDHPAVVMIAFTGSREVGLRIWESAGKTLPGQREL
                     KRVVCEMGGKNAIIVDSDADLDETIVDSIYSAFGYQGQKCSALSRLIVLEENYDRVME
                     RLLAAAASLRVGNPEEPGITVGPVVDDRAYKRILEMIEVGKKEATVAFQARDVPKDGY
                     FIPPTIFADVKPTARIAREEIFGPVLAVIKVRDLDEAIAVANSTDYALTAGFFSRSPA
                     NIERVKAELEAGNVYINRSCTGAVVGRHPFGGFKMSGGGTKAGGRDYLLQFLIPRVVT
                     ENIMRHGFAPEETPRHRDEFLVRRASPAAGEKNSAHS"
     gene            3850..4914
                     /locus_tag="VNW28_04710"
     CDS             3850..4914
                     /locus_tag="VNW28_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HXA09258.1"
                     /translation="MPTRPRHQFASDNTAAICPEAWAALAQANQDGAASYGEDRWTAR
                     VRDQVREIFETDCDTFLVFNGTAANALALAQLCQPFHSVLCHERAHIETDECGAPEFF
                     SGGTKLLRVGGKNWKLDLEEAETVMAQHRDVHSTKVRTLSLTQATEGGTVYVPEELDA
                     IADFAAKHSLAVHMDGARFANAVASLGCAPKEITWQRGVNVLSFGGTKNGVAAGELVV
                     FFDRARAHEFEYRAKQGGQLASKTRFLAAPWTGLLGSDVWLKNARHANECARLLEEKL
                     RAAGFSDLVFPREASALFVRLPNEIVERVRARGWQFYRFIEPDVYRLMCSWSVTKEDI
                     EEFVADFRNAQQPALLNVGR"
     gene            4940..7615
                     /locus_tag="VNW28_04715"
     CDS             4940..7615
                     /locus_tag="VNW28_04715"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA];
                     GO_process: GO:0008380 - RNA splicing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HXA09259.1"
                     /translation="MTPRDLPIYELENEIVGSLVSQPRLILQAPTGSGKSTQVPQILL
                     ERGLLGTGEVVILQPRRLATRMLASRVAFERNGRLGDEVGYQIRFEKVASKNTRIRFV
                     TEGILLRQLLQDPELRGIAAILFDEFHERHLYGDITLARTLYLQETQRPELKLVVMSA
                     TLESDKLEKYLAPCPVLNSSGRTHPVRVEYLTKPVRGEGYPIWDLAADEFERLAAQTE
                     GDVLVFMPGKYEITRTISAIRASRVSDRFVVLPLHSELPPAEQDAALKPYEKRKVIVA
                     TNVAETSLTIDGVRVVIDSGLARIARFDPRRGINTLFIEKISRASSEQRAGRAGRTAP
                     GHCLRLWTESEHLERPAQELPEVKRLDLAEVVLTLKASGIEDVAGFSWLEAPDPKALE
                     NAEQLLTDLGALSGSARVSRAGCGVPPQRTFPVRPTPDKITNATKVRDGETPSPAHET
                     YALPGITALGRRMLAFPVHPRYARMLLAAQEQGCVRGVALIAALTQGRNLLGRAEGKQ
                     VRDEREDFLGGQEESDLFILLRAFRYAEKNRFDPRRCARLGVNAGAARDAGQLWEQFL
                     SIARAEGLDIAEREAEPGAIARCVLAGFPDQVAARLDEGTLRCALVHNRRGVLARESV
                     VHRARLLTASEIREIESSDGERQVLLTLATAIKEEWLREFFPDNVRERTEVTYDPSLR
                     RVVGRTVTLFHDLVLAEKKSERVPAAEAAALLAREVIAGNCPLKNWDHAIEQWIARLN
                     FAAAAFPELELPPIGEADRALLIEQVCQGATSYKEIKDRPVGPVLKSWLSAGHQRALD
                     QLAPERIKLPNERNAKISYSASQAPTIAARIQDLYGVENGLTIGQGRVTLRIEVLAPN
                     HRPIQITDDLATFWRESYPKIRQELQRKYPKHQWR"
     gene            7797..8291
                     /locus_tag="VNW28_04720"
     CDS             7797..8291
                     /locus_tag="VNW28_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09260.1"
                     /translation="MKNSNGDNFMEHDPMAETEFGSEMTGRGGESRMQEGARKISSAA
                     ADTWEQTRQKALVAREKTEFFLRENPVPTVLGALALGLAIGLAIRYSSRPEEPSLTLK
                     RALREIDWSTISPAFLPGLIRSAKRGYADSAEAVKEGLDRVKEVDVDGYVKPLRKQWK
                     KWMS"
     gene            complement(8408..>9383)
                     /locus_tag="VNW28_04725"
     CDS             complement(8408..>9383)
                     /locus_tag="VNW28_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HXA09261.1"
                     /translation="WRELLADFSPDALQAWAEELAVETYVGTSKRVFPRGQKAAVLLR
                     AWLQRLRSAGVEFRTGARLLGLTREDDSWRLDFGGGGRMVARAVVLALGGASWPETGS
                     DGGWLTLLAAHGVEMAPFAAANCGWNVSWPAAVLERAEGLPLKNLNVTAAGEVVSGEL
                     LITRYGLEGGAIYRLGPVLREMARPELRIDFKPQVTADGLRERAAQLSAPNEWFRAWK
                     LSAGVVALVESHFPEDCVEREKAIARLKEFPLPLGGPRPIAEAISTAGGVLWRELDDQ
                     LMLRKMPGVFLAGEMIDWEAPTGGYLLQGCFSTGTRAGRAAAKYARRP"
BASE COUNT         1781 a         3027 c         2851 g         1724 t
ORIGIN      
        1 cccatttcgc aaggaaaaca aaatgaaacc caatgaccag attgaatcgg taaagtggtt
       61 gtcccgttat gacgacccca cccggcgcgg cggttcgctc ccgcgcatca catttgaata
      121 tcagcagcgc cgcggaggcg gttcgacttt tggcggcgcc agccccgcgc gccagcccgg
      181 atttacccgg ctcgccaatg aagtcctgcg ctccgatgcg agccgcagct ttcggctgga
      241 aacggccgtc ctcggcttcg tcaccctcgt ttcggcctgg ccgatcgcgg tcatgattca
      301 cgaggtcatc cggctgctac agtaagcttt gcgccattaa tctggctcgc tccggggtca
      361 catccccgga gcgacgcccg ctgaggcctg tcgtcagctg tgcgccgaat tcttctcccc
      421 cgccgcgggg ctcgcccgcc taacgagaaa ctcatcgcga tgccgcggcg tctcttccgg
      481 ggcgaaaccg tggcgcatga tgttctcggt cacgacccgc gggatgagga attgcagcaa
      541 gtaatcgcgc ccgcccgctt tcgtcccccc gccgctcatc ttgaacccgc caaagggatg
      601 acgcccgacc accgcccccg tgcaggaacg attgatgtag acgttaccgg cttccaactc
      661 tgctttcacc cgctcgatat tggccgggct gcgcgagaaa aagcccgcgg tcaacgcgta
      721 gtcggtacta ttcgcgaccg cgatggcttc atccaggtcg cgcactttga tcacggccag
      781 gaccggaccg aaaatttctt cgcgcgcgat ccgggcggtc ggtttcacgt ccgcgaaaat
      841 ggtcggcggg atgaagtaac catccttcgg cacgtccctt gcctggaaag ccacggtcgc
      901 ctctttcttg ccgacttcga tcatctcgag gattcgtttg taggctctgt cgtcgaccac
      961 cggaccgacg gtgatacctg gttcttcggg atttccaacg cgcaaactcg cggccgcagc
     1021 cagcaggcgt tccatcactc gatcgtagtt ttcttcgagc acgatgagtc gggagagcgc
     1081 ggaacatttc tgaccctggt aaccgaaggc ggaatagatc gagtcgacga tcgtctcgtc
     1141 gaggtcggcg tcggaatcaa cgatgatggc gttcttgccg cccatctcgc agacgacgcg
     1201 cttcagttcg cgctggccag gcagtgtctt gccggctgac tcccaaatgc gcaggccgac
     1261 ctcgcgcgag ccggtaaacg cgatcatcac gaccgcagga tgatcgacca gatgttgccc
     1321 gacgacggag ccgtggcccg gcagataatt gaggacgccc ggcggcaccc ccgcttcctc
     1381 gaacatctcc atcaagagcg ctccggtaac ggacgatggc tctgccggct tcatgatgac
     1441 ggtgttgccg gtcacaatcg ccgccgagac catgccggtg agaatggcga tcgggaaatt
     1501 ccacggcgca atcacgagcg cgacgccgcg tggccagtaa tgctggtagc tttcttcgcc
     1561 gagcacgtgc tgggtgagtt tcggccggcc catgccgcgc atttgctggg cgtagaagcg
     1621 gcagaaatcc accgcctcgc gaatgtcgcc atctgcttcc tcccaggcct tgccgacctc
     1681 gtaaacttcc acggccgcca gctccaaacg ccgccgcgcc atgatggccg cgacgcgctc
     1741 gagcaattgc gcacggtgct cgatcgacac ccggcaccaa tgttcaaaag ctgcggaggc
     1801 tgccttgact gcgcgttccg cttcgggaac tcccgcttcc gcgacctcgc ccacgatctc
     1861 gttaggtgcg ctcggattga ccgacgccat ccactttccg gtcgtgaccc tttcgccgcc
     1921 gatgacgagc ggatacctct tgccaaactg gctgcgcgcc gcgcgcaacg ccgttttcat
     1981 ctgctcctgg gtttggcggt gaacaaagtt gacggctggc gcgttttcgt aggcggcgcc
     2041 atcggcggat ggttcctggt tcggctgcac tttggttggc tcctcggttg tgatcagttc
     2101 cttcgggtca cgcagtaact gcgcggcgga ctggtggtcg gaaaatttcg cccgcagaaa
     2161 accttcgttc gacgtgttct cgagtaatcg cctaacgaga taggacatcc ccggcagcaa
     2221 ctcgccaacc ggcgaatact cccgcacgcg atggcccatc tcgaccagcg cgcgcttgat
     2281 cggccccgcc attccataaa gcagctgaaa ttcaaagcgg ctctggtcga tcccgagctc
     2341 ttccgcacag gccatcgcgt gggcgatgct gcgcacgttg tgcgagccga aggcggcggt
     2401 cacgaccgat tcgttctcca gcaaaatgcg ggtgcaagtt tcgaagctgg cgtcgctttc
     2461 cggtttttgc agaaacaccg ggcaaaccca gccgttctgt tgcgcgacga tcttttcgta
     2521 atcccaatag gcgcctttca ccaggcgcac ggcgaagcgc gtcccgcgcc ggcgtcccca
     2581 ctcgatcagg tcgcgcaggt caggttcccc gtcgcgcagg taggcctgga cgacgatccc
     2641 gacatgcggc caatccgcga actccggctc ggtgaagagc tgcttgaaca agtcgagcgt
     2701 gccgttcttc tgcgcgtagc tctccatgtc gaaattgacg aaggccccca cttccttcgc
     2761 ccggcgcagg atcggccgca gcttcggtgc gagatgcgca accgcttcct cgggcgccgc
     2821 cgggtccatc tgggaataga atgccgagat ctttagcgaa agattaacga ccggaaatag
     2881 ctcggcgttt ttgcctaacg gatcggtcca gccgcgcgtc tcccgcgaaa gggtctcgag
     2941 caactccatg gcgcgcgccg cgtactcgtc cgcttccgtt tcgctgacga ccgcttcgcc
     3001 taacaagtcg accgtgaagc cgatcttctc ctttcgcttg cgccggagcg tcttcatcac
     3061 gtcggcgccg tccttcccag cgataaactg gcgcgccatc ccggagacgt tgcggcgcag
     3121 gacgaaagca gtcagccagg gaaaaatgcc ggccgcgcgc agaccggtct gcaacattgg
     3181 aacgacgccg tccatcccat gaaaatattc acgcagatgg accacgatat cgcttgagcg
     3241 ccggagcgac gcgagcacat cgacgaagcg aaacatttgc accttgaagg tttcgtcgcg
     3301 catcgagaag gccatcatct tctgataaaa gccggctttg ctgaagatcg actccggata
     3361 ctggtcgacc agttcgaaaa ttcgttgacc gcgacgttcg gtcgcggctt gcagagcgct
     3421 catggggcga ggataaagcg gcccgcgcct ttcggcaaag ctggagacgc ttccccccag
     3481 ggagcgcggg gatgagaccc gatcggagaa ccgctctcgc gttttcgccg agcggaacgt
     3541 ctccaatcct gcgtcggcgc acgatcaatc ccgcgctctc ttccgaataa tcggtgaaac
     3601 aattttacca tgaaccttcc ccaacgagtc aaggaagccg gcgcagccgg atacgtttta
     3661 ctgtggctcc tcggcgttcc gatcccggtt ttgttcgtca tcttcctgct gcgtggttgc
     3721 agctagctgg gcaaggaaga ggtgagttgc ctggccggga gctccgatct cgcgatcggc
     3781 tcctggtcag gttccggttt tcgttaggcg atttgccgcc gcgcgcaatg tcagctaggg
     3841 atgttcccga tgccgaccag gccccgccat caatttgcca gcgacaatac ggccgccatt
     3901 tgcccggaag cctgggctgc cctcgcccag gccaatcagg atggcgcggc ttcctacggc
     3961 gaggatcggt ggaccgcgcg cgtccgcgac caggtacgcg agattttcga gaccgactgc
     4021 gacaccttcc tggtcttcaa cggcacggcc gccaatgcac tcgcgctggc gcagctttgc
     4081 cagccgtttc acagcgtcct ttgccatgag cgcgcccata tcgagaccga tgaatgtggc
     4141 gcgccggagt ttttttccgg cggcaccaag ctgctgcgcg tcggcgggaa aaactggaag
     4201 ctcgacctcg aagaagccga gacggtcatg gcccagcatc gtgatgtgca ctcgaccaaa
     4261 gtccgcaccc tgagcctcac tcaggcgact gaggggggca cggtttacgt tcccgaagag
     4321 ctggacgcga tcgccgattt tgccgccaaa cattctctcg ccgtgcacat ggatggcgct
     4381 cgttttgcca acgcggtcgc ctcgttaggc tgcgccccga aagaaatcac ctggcaacgc
     4441 ggtgtgaatg tcctctcctt cggcggaacg aaaaacggcg tcgccgctgg cgaactggtt
     4501 gtttttttcg atcgcgcgcg ggcgcacgag ttcgagtacc gggccaaaca gggcggacag
     4561 ctcgcctcga agacgcgctt cctcgccgcc ccgtggaccg gcttgctggg cagtgacgtc
     4621 tggctgaaaa acgcgcgtca cgccaacgag tgcgcgcgtt tgctggaaga aaagttgcgc
     4681 gcggcgggct tttcagatct cgttttcccc cgagaggcga gtgcgctttt cgtgcgcttg
     4741 cctaacgaaa tcgtcgaacg ggtaagggcg cgcggctggc aattctatag attcatcgaa
     4801 cccgatgtct accggctgat gtgttcctgg tccgtcacga aagaggacat cgaggaattt
     4861 gtcgccgact tccggaatgc tcagcaaccg gcgttgctga acgttggacg ttgagcgctg
     4921 gacgttgaac gttcgccgga tgaccccgcg cgatctcccg atctatgaac tggagaacga
     4981 gattgtcggg tcactcgtta gtcagccgcg tctcatcctg caggcgccga ccggttccgg
     5041 aaaatcaacc caggtgccgc agatcctgct cgagcgcggc ctcctcggca cgggcgaagt
     5101 cgtcatcctg cagccgcgcc ggctcgccac gcgcatgctc gccagccggg tcgcattcga
     5161 acgcaacgga cgcctcggcg acgaagtggg ctaccagatt cgcttcgaga aagtcgcgtc
     5221 caaaaacacc cggattcgct ttgtcaccga gggaattctc ctgcgacaat tgttgcagga
     5281 tccggaattg cgcgggatcg cggcgattct cttcgacgaa ttccacgagc gccatctcta
     5341 cggcgacatc accctcgcgc gcacgctcta tcttcaggaa acgcagcgac ccgagctcaa
     5401 gctcgtggtc atgtccgcga ccttggagtc ggacaagctc gagaaatacc tcgcgccctg
     5461 tcccgtcctg aactcgagcg ggcgcacgca cccggtgcgg gtcgaatacc taacgaaacc
     5521 agtacgcggc gaaggttatc cgatctggga cctggcggcg gatgagttcg agcgtctcgc
     5581 ggcgcagacc gaaggcgatg tgctcgtctt catgccgggg aaatacgaaa tcacccgcac
     5641 catctcggcc atccgtgcct cgcgggtgag cgatcggttt gtcgtcctgc cgctgcacag
     5701 cgagttgccg ccggccgagc aggacgcggc gctcaaacca tacgagaagc gcaaagtgat
     5761 cgtcgcgacg aatgtcgcgg aaacctctct cacgatcgac ggcgtgcggg tggtgatcga
     5821 cagcggcctc gcgcggatcg cgcgtttcga tccgcgccgc gggatcaata ccctcttcat
     5881 cgagaaaatc agccgcgcgt cgtccgagca acgcgccggc cgcgccgggc gcacggcgcc
     5941 cggacattgc ctgcgactct ggaccgagag cgaacatctg gagcgcccag cgcaggaact
     6001 tcccgaagtg aagcggctcg atctcgcgga agtcgtcctc accttgaagg cgagcgggat
     6061 cgaggacgtg gccggattta gttggctcga agcgcccgac ccgaaggcgc tggaaaatgc
     6121 ggaacaattg ttgaccgatc tcggcgccct gtcgggtagc gcacgcgtct cgcgtgctgg
     6181 ttgcggcgtt ccgccgcaac gaacttttcc agtaagaccc acgccggata agatcacaaa
     6241 cgcaacgaaa gttcgcgacg gcgagacgcc gtcgccagca cacgagacgt atgcgctacc
     6301 cggaataact gccctcggcc ggcgcatgct cgcctttccg gtgcatcctc gttacgcgcg
     6361 catgctcctc gccgcccagg agcagggttg cgtccgcggc gtcgcgctca tcgcggcgct
     6421 gactcaggga cgcaacctgt tgggccgcgc cgaaggaaaa caggtgcgcg acgaacgcga
     6481 ggatttcctc ggcggccagg aggaatccga tctcttcatt ttgcttcgtg cctttcgtta
     6541 tgccgaaaaa aatcgcttcg atccgcgacg ttgcgcccgg ctcggagtga acgccggcgc
     6601 ggcgcgcgac gcagggcaac tgtgggagca gtttctctcc atcgcccgcg ccgagggcct
     6661 ggatatcgcg gagcgcgagg ccgagcccgg cgcgatcgcg cgctgcgtcc tcgcgggatt
     6721 tccagatcag gtcgcagcgc gcctcgacga aggaacgctg cgttgcgcgc tcgtgcataa
     6781 tcgccgcggc gtgctcgccc gcgaaagcgt cgtccatcgc gcccggcttt tgaccgcctc
     6841 agagattcgc gaaatcgagt cgagcgacgg cgaacgccag gtcttgttga ccctcgcgac
     6901 cgcgatcaag gaagaatggc tgcgcgaatt ctttccggac aatgtccggg aaaggaccga
     6961 ggtaacctac gatccatcgc tccgccgagt cgtcgggcgg accgtcacac tttttcacga
     7021 tctcgtcctc gcggagaaga aatcggagcg cgtcccggcg gccgaggccg cggcccttct
     7081 ggcgcgggaa gtgatcgcgg gaaattgtcc gctgaagaac tgggaccacg cgattgagca
     7141 atggatcgcg cggctcaatt tcgcggccgc ggcgtttccc gaactcgaac tcccgccgat
     7201 tggcgaagcg gaccgcgcat tgttgatcga gcaggtctgc cagggcgcga cgagctacaa
     7261 ggagatcaag gatcgccccg tcgggccggt cctgaaatcg tggctgagcg cggggcacca
     7321 gcgcgcgctc gaccagctcg cgccggagcg gatcaaattg cctaacgagc gtaacgcgaa
     7381 gatcagctac agcgcgagcc aggcgccgac gatcgcggcg cgcatccagg acctctacgg
     7441 ggtggagaac ggtctgacaa tcggtcaggg ccgggtaacg ctgcggatcg aagtactggc
     7501 gccgaatcat cgcccgatcc agatcaccga cgacctcgct accttctggc gcgagagtta
     7561 tccgaaaatc aggcaggagc tgcagcgcaa gtatccgaag caccagtggc gctgattgca
     7621 gccgggattg ctgatcccgg ctacaatcaa atgctgccgc gaataccgcg ggagggatcg
     7681 cgtggagaaa tcagaccgct gacctggacc aggcggcctt cttccgcggc gacgcccgct
     7741 catcgggaaa cgcgaattca ggggcgcaaa acgcgcgcga tatgcgaagg ttaagtatga
     7801 agaattccaa tggagacaat tttatggaac acgatccgat ggcggaaacc gagtttggca
     7861 gcgagatgac cggtcggggc ggcgaaagcc ggatgcagga gggcgcgcgg aagatttcca
     7921 gcgccgcggc cgatacttgg gaacagactc gtcagaaggc gctcgtcgcg cgcgagaaga
     7981 cggagttttt cctccgggaa aacccggttc cgaccgtcct cggcgcgctt gctctcgggt
     8041 tggcaattgg tttggcgatc cgttattcct cacgacccga ggagcccagc ctgacgctca
     8101 aacgcgctct gcgtgagatc gattggagca cgatctcgcc cgccttttta cccggcctca
     8161 ttcgcagcgc aaagcgcggc tacgcggatt ccgctgaggc ggtcaaggaa ggtctcgacc
     8221 gggtcaaaga ggtagacgtg gacggatacg tcaaaccgtt gcgcaagcag tggaagaagt
     8281 ggatgagcta gccaggcaaa ttcgttaggc gagcggcgct cttggtcggc gccgctctct
     8341 ggacggcgca agagtgtgcg ttcttcttgc tgcggcgccg aggcccgcgc tccgcagagc
     8401 aaagcattta cggacgtcgc gcatacttcg ccgccgcgcg accggcgcgc gtcccggtgg
     8461 aaaagcaacc ctgcaggagg tagccgccag ttggcgcttc ccagtcgatc atttctccgg
     8521 cgagaaaaac gcccggcatt ttccgcagca tcagttgatc gtccaattcc cgccacaaaa
     8581 ccccgcccgc ggttgagatc gcttcggcga tcggccgcgg acctcccaac ggcaacggaa
     8641 attccttcag gcgcgcgatc gctttctcgc gctcgacgca atcttcgggg aaatggcttt
     8701 ccacgagcgc gaccactccc gcgctcaatt tccaggcgcg aaaccactcg ttaggcgcag
     8761 acaattgggc ggcgcgttcg cgcaggccat ccgccgttac ctggggcttg aaatcgattc
     8821 gcaattccgg ccgggccatc tcgcgcaaga ccggaccgag ccggtaaatc gcgccgcctt
     8881 ccaaaccgta gcgcgtaatc aacaactcgc cggaaaccac ttcgccggcg gcggtgacat
     8941 tgagattctt gagcggcaga ccttccgcgc gttcgagcac ggccgcgggc cagctcacat
     9001 tccagccgca attggcggcg gcgaaaggcg ccatctccac gccatgggcg gcgagcaaag
     9061 tgagccatcc gccgtccgaa ccggtctccg gccaggaggc gccacccagg gcgagaacca
     9121 cggcgcgggc gaccatccgt ccgccgccac cgaagtcgag ccgccaggaa tcgtcttctc
     9181 tcgttaggcc aagcaaccgt gcgccggttc tgaactccac tccggcactc cgcagccgct
     9241 ggagccaggc gcgcaggagg acggcggctt tctggccgcg cggaaaaacg cgtttgctcg
     9301 tgccgacata tgtctcgacg gccagctcct ccgcccacgc ctgcaacgcg tccgggctaa
     9361 agtccgccag caactcgcgc cac
//
LOCUS       DATMNS010000129         5765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4394982, whole genome shotgun sequence.
ACCESSION   DATMNS010000129 DATMNS010000000
VERSION     DATMNS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4394982"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VNW28_04730"
     CDS             complement(<1..1037)
                     /locus_tag="VNW28_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXA09262.1"
                     /translation="MASKLFQTKSPEHLIRESEAPDRKMKRSLTALDLTCLGIGAIIG
                     AGIFALAGTAAAGESVHVGESIVKTPVLNFIIAFINHTDLVFGRPAAGPAVAISFVVA
                     AIACGFAALCYSELAAMIPVSGSAYTYSYATLGEIIAWIIGWDLILEYAVGNMAVAKG
                     WSDYFVKLLYSLSHGAIKLPLWLVTDPTTAATKLAQGGEASQYYSPLVLPHLFGYPIA
                     LDLPAVLIVAAVTVLLIYGIRESATTNTTIVLIKVAVVVFVIAFGSFMVHPANWQPFA
                     PSGFGGIMGGAAIVFFAFIGFDAISTTAEETKNPQRDLPIGMIASLIICTLLYVLMSV
                     VLTGIKKYTVYL"
     gene            1186..1407
                     /locus_tag="VNW28_04735"
     CDS             1186..1407
                     /locus_tag="VNW28_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09263.1"
                     /translation="MPSLSGLSGPNLFAGLLFSSIGFVGFVYGRKMSAWRPMFLGIAL
                     MAYPYFIEDTITICAIGLAGSAALCYFRD"
     gene            1471..2538
                     /locus_tag="VNW28_04740"
     CDS             1471..2538
                     /locus_tag="VNW28_04740"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HXA09264.1"
                     /translation="MADETTPLVFGDAAASELGNYPGRKWLRGKRKARASLTHCENCG
                     APLSGPYCAVCGQPAIDYHRSFGSLLADAADAFFNLDARFFRSFGLLLLKPWRLTNEF
                     VAGKRARHVHPLRVYLIASVIFFLVINFLSKDAHLEANASDDHSGLSITSATPTPGPL
                     FPVIPSPGFSSGLSFLPSPTAAPSPQPQDVLIGFDGAENKSPFAIWVQKRAKEKLGPS
                     GDRGDLFLKALLQNLAPMVLCCIPLFAFVLKILYIFKRRYYIEHLIFALHTHAFVFLS
                     TVIIIGLGFLVALKSTALTAVICTLFGFAVVIQLLVAIRRVYRQNWLATLFKFALGSV
                     IYLVLLSIAFGVTAVLTLLLP"
     gene            complement(2615..3409)
                     /locus_tag="VNW28_04745"
     CDS             complement(2615..3409)
                     /locus_tag="VNW28_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXA09265.1"
                     /translation="MDLKLQDKKAFVSGSTAGIGLAIASRLAREGAEVWLNGRTQARV
                     EQAIAKIKKEFTNLRLNGIAADLGTAQGCAEVVRQLPQVDVLVNNVGIFEPKPFEKIG
                     DEEWEKFFAVNVLSGVRLSRAYLGGMKERNWGRIIFISSESGLQIPAEMIHYGTTKTA
                     QLAVARGLAETCAGTKVTVNSVLPGPTASEGVTEFVGQLAQENKMSPEEFEKDFFKNA
                     RPTSLLKRFIEPEEIADVVAFVCSPLAAAINGASVRADGGVIKSLA"
     gene            3545..4276
                     /locus_tag="VNW28_04750"
     CDS             3545..4276
                     /locus_tag="VNW28_04750"
                     /inference="COORDINATES: protein motif:HMM:NF009044.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXA09266.1"
                     /translation="MGRQWLHAKREVAGLKKGQAVGKLVKEIMVAAKMGGGDPSGNPR
                     LAAVIEKARKQSVTRDTIERAIKKGAGIGGEKLELEHVVFEGYAPRKVPVVVEVLTDN
                     KNRTAPEIRVLFKRGQLGAAGSNKFLFDHVGLVEAYHVDPLADVEAAAIEAGAQEVEP
                     LTSGQNEDIPEGTAGARFTTAATDLHAVSQWLAQNQWTVVTSELAYIAKSFPELSDED
                     RTQVGEFLQLLEDHDDVHRVWAAIR"
     gene            4285..5394
                     /locus_tag="VNW28_04755"
     CDS             4285..5394
                     /locus_tag="VNW28_04755"
                     /inference="COORDINATES: protein motif:HMM:NF023828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HXA09267.1"
                     /translation="MSDETTQAIFGDAVADSLEKPGSRRRGLFGRKRPAKDEPSRFTH
                     CEDCGAELHGRYCSKCGQVAVDYHRSFRHVVADVAESFLNWDSKFTKTIGLLVIRPGW
                     LTNQFVAGRRTRYLHPLRLYLLVSILFFLCVRLAPIETKPPRGANITAKDRAKVEEAM
                     KDPNIPAAVREKVLRGMAESAKSSAKQTGPVLPAEVPSPSPSPVVRGHSKLLDDGSHS
                     SWLKERIKEKIGPNGANVQLLGKTLRDNLSTMMLFCIPLFALVLKILYFRQRRYYIDH
                     LVYALHIHSFFYIAILLTVFSAIGAKHWAPALETPLLWILWLAIVAQVFLSIRQVYRQ
                     GWSMTIFKFFLGGFFYLFVLIVALAATAVATIALL"
     gene            complement(5482..>5765)
                     /locus_tag="VNW28_04760"
     CDS             complement(5482..>5765)
                     /locus_tag="VNW28_04760"
                     /inference="COORDINATES: protein motif:HMM:NF014342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HXA09268.1"
                     /translation="PDADGKLLQAVADALRTRFEGPIFLAGEKDGRVALIAAIPKELT
                     ARFQAGKLIQETAPLIGGKGGGRPELAQGGGTDATKIEAALAKARALLS"
BASE COUNT         1082 a         1746 c         1692 g         1245 t
ORIGIN      
        1 agataaaccg tgtatttttt aatgccggtc agcacgaccg acatgagcac gtaaagcaac
       61 gtgcagatga tcaggctggc gatcatgccg atcggcaaat cgcgttgcgg attcttcgtt
      121 tcctctgctg tggtggagat ggcatcgaaa ccgatgaagg caaagaaaac aatcgcggcg
      181 ccgcccatga tgccgccaaa tccgctcggc gcgaagggct gccagtttgc cgggtgaacc
      241 atgaacgagc cgaaggcgat gacgaaaacc accaccgcga ccttgatcaa aacgatcgtc
      301 gtgttggtcg tcgcgctttc gcgaatcccg taaatgagga ggaccgttac cgcagccacg
      361 atcaataccg ccgggagatc gagggcgatg gggtaaccaa ataggtgcgg caaaacgagc
      421 ggcgaataat actgcgaagc ttcaccccct tgggcgagct tcgtcgccgc cgtcgtgggg
      481 tcggtcacca accaaagcgg aagttttatt gcgccatgac tcaggctgta gagcagcttg
      541 acgaaataat ccgaccagcc tttcgccacg gccatgttgc cgacggcata ttcaaggatc
      601 aaatcccaac caatgatcca ggcgatgatc tcaccgaggg tcgcgtagga gtaagtgtaa
      661 gctgatcccg aaaccggaat catggccgcg agttcgctgt agcagagggc ggcgaaaccg
      721 caggcaatgg ctgctacgac gaaggaaatc gccaccgccg gccccgccgc ggggcgaccg
      781 aagacgaggt cggtgtggtt gatgaatgcg atgatgaaat tgagtaccgg cgttttcacg
      841 atcgattcgc cgacgtgcac gctctcgccc gcagcggccg tgccggcaag cgcgaaaatc
      901 ccggcgccga tgatggctcc gatgccgaga caggtcagat cgagcgcggt gagcgagcgc
      961 ttcatcttgc gatccggcgc ttccgattcg cggatcaaat gttcggggct ttttgtctga
     1021 aaaagtttgg atgccacaga ttttctccgg ttgtcaggct gaggtttccc gaaagtgggc
     1081 gcatcgtcgc acccggcgat tgcgccggtc aatgcttgtt ttggacgcgt tataaccggc
     1141 agccatgacc cctctggctg gcgcggattt agctgttaat agaggatgcc ctctctctcc
     1201 ggtctctccg gccccaatct tttcgccggc ctgctcttca gcagcattgg tttcgtcggc
     1261 ttcgtctatg ggagaaagat gagcgcctgg agaccgatgt ttcttggcat cgcgctgatg
     1321 gcatatccct attttatcga agacacgatc acgatttgcg ccatcgggct cgccggatcg
     1381 gccgcgctct gttattttcg ggattgagac gcgggagtcc gggccgcttc cggatggact
     1441 tgccgcgagg agttgaatac tctgcggccg atggccgacg aaacgacccc gcttgttttc
     1501 ggcgatgccg cggcttccga gctcggcaat tatcccggac gaaaatggct gcgggggaag
     1561 agaaaggcac gggcgtcgct gacccattgc gaaaattgcg gcgcgccgct gagcgggccc
     1621 tattgcgcgg tctgcggcca gccggcgatc gattatcatc gctccttcgg ctcgctcctg
     1681 gccgatgccg ccgatgcctt cttcaacctc gacgcgcgtt ttttccggtc cttcggacta
     1741 ctccttctca agccgtggcg cctgacgaac gagttcgtgg ccggcaagcg cgcccgccac
     1801 gttcatcccc tgcgcgttta tctcatcgcg agcgtcatct tcttcctcgt catcaatttc
     1861 ctttccaagg atgcgcacct cgaagccaac gcgagtgacg atcattccgg gttgtcgatc
     1921 acctccgcta cgcccacgcc cggcccgctc ttccccgtca ttccctctcc cggtttttcg
     1981 tccggcttgt ccttcctccc atctccgacc gccgcgccca gcccgcagcc gcaagacgtt
     2041 ctgattggct tcgatggcgc cgaaaacaag tcgccttttg ccatctgggt gcaaaagcgc
     2101 gcgaaggaaa aactcgggcc gagcggcgac cggggcgacc tctttctcaa ggcgctgtta
     2161 caaaacctcg cgccgatggt gctctgctgc atcccactct tcgcgttcgt cctgaaaatc
     2221 ctctacatct tcaagcggcg ctactatatc gagcacctca ttttcgcgct ccatacccac
     2281 gccttcgttt ttctcagcac cgtgatcatt atcggcctcg ggtttctggt ggcattgaaa
     2341 tcgacggcgc tcaccgccgt catctgcacc ctttttggtt tcgcggttgt gatccagttg
     2401 ctcgtcgcga ttcggcgggt ttaccgccag aactggctgg ccactctttt caagttcgcc
     2461 ctcgggagcg tgatctacct cgtgctcctg agcatcgctt tcggcgtgac cgccgttttg
     2521 accctgcttc tgccctgagc cgtgaagctg gacttgcgga agcaagtccc tccgcaagga
     2581 ccagtttccg caagtcccgt ttgggagctg ctcgttaggc aagcgacttg atcactccgc
     2641 cgtcggcgcg cacggacgcg ccgttgatcg cggccgcgag ggggctgcaa acaaatgcga
     2701 ccacgtccgc gatctcctcg ggttcgatga agcgtttcag aagcgaggtt ggccgcgcgt
     2761 tcttgaaaaa gtctttctcg aactcctccg ggctcatctt gttttcctgc gccagctgcc
     2821 cgacgaactc ggtcacgccc tccgacgcgg tcgggccagg aaggacggag ttgaccgtca
     2881 ccttcgttcc agcgcaggtc tctgccaggc cgcgtgcgac cgcgagctgc gcggtcttgg
     2941 tggtcccgta atggatcatc tccgccggga tctgaagtcc cgattcactc gagataaaaa
     3001 tgatccggcc ccaattgcgt tccttcattc ctccgagata ggcgcggctc agccgcacgc
     3061 cggagaggac gttgacggcg aagaacttct cccattcttc atcgccgatt ttttcaaacg
     3121 gcttcggctc gaagatgccg acattgttga ccagcacgtc gacctgcggc agttgcctaa
     3181 cgacttcggc gcaaccttga gcggtaccga gatcggccgc gatgccgttc agcctgaggt
     3241 tggtaaattc ctttttaatt ttcgcgatcg cttgctcgac gcgcgcctgc gtccgtccgt
     3301 tcagccagac ttccgcgcct tcgcgcgcca gccggctcgc gattgccagc ccgatcccgg
     3361 cggtcgagcc gctgacgaag gctttcttgt cctgcaattt caaatccatg ggatttcctt
     3421 ttccacgcgg ccggaaccgt tcaaggaaat tcgatcggca gggtccaacc ttgacaaggt
     3481 gagctccgaa ggctttcgcg agcagcgctt gccctctggc tgccatccgc gttaaatcag
     3541 cggcatgggt cgtcaatggc tgcacgccaa gcgagaagtg gccggtctga agaaaggcca
     3601 ggcggtgggc aagctggtga aggaaatcat ggtggcggcc aagatgggcg gcggcgatcc
     3661 ctcgggcaac ccgcgtctcg cggccgtcat tgagaaggcg cgcaagcaga gcgtgacccg
     3721 agacacgatt gagcgcgcga tcaagaaagg cgcggggatc gggggcgaaa aactcgaact
     3781 tgagcatgtc gttttcgaag gctacgcgcc gcgcaaggtt ccggtggtcg tggaggttct
     3841 aaccgataat aagaaccgca ccgcaccgga gatccgcgtt cttttcaagc gcggtcagct
     3901 cggcgcggcc ggcagcaaca agtttctttt tgatcacgtc ggattagtcg aagcgtatca
     3961 tgtcgacccg ctcgcggacg tggaagccgc tgcgatcgag gccggcgcgc aggaagtcga
     4021 gccgctcacg tcgggacaga atgaggatat cccggaagga acggcgggcg cgcgtttcac
     4081 gacggcggcg accgacctcc atgcggtctc gcaatggctg gcgcagaatc agtggacggt
     4141 ggtcacgagt gagctcgcgt acatcgcaaa gagttttccg gagctcagcg atgaggatcg
     4201 aacgcaggtg ggtgaatttc tccagctcct cgaggaccac gacgacgtgc atcgggtctg
     4261 ggcggcaatt cgttaggtga agcaatgtcc gacgagacga cccaggcgat tttcggcgac
     4321 gcggtggccg actcgctgga aaaaccgggc agccggcgcc gcgggctctt cggccggaag
     4381 agaccggcga aggacgaacc ttcgcgcttc acccattgcg aagactgtgg cgcggaattg
     4441 cacggccgct attgctcgaa gtgcggacag gtggcggtcg attaccaccg ttcctttcga
     4501 cacgtcgtcg ctgatgtggc ggaatccttc ctcaactggg attcaaaatt taccaagacg
     4561 attgggttgc tcgtcattcg acccgggtgg ctgaccaacc aattcgttgc tggccggcgc
     4621 acacgctatc tccatccttt gcgtctttac cttctggtca gcatcctttt ctttttgtgc
     4681 gttcgcctcg ccccgatcga gaccaagccg ccgaggggag cgaatataac ggcgaaggat
     4741 cgcgcgaaag tggaggaggc gatgaaggat ccgaatatcc cggctgcggt gcgggaaaaa
     4801 gtgctgagag gcatggccga gagtgcgaag tcatcagcga agcagaccgg cccggtcttg
     4861 cccgccgagg tcccatcacc gtcgccgtcg ccggtcgtta ggggccactc gaagctattg
     4921 gacgacgggt cgcactcgag ctggctgaaa gaaaggataa aagaaaagat cggccccaac
     4981 ggagccaacg tgcagctcct aggcaagacg ctccgcgaca atctctcgac catgatgctt
     5041 ttctgcatcc cgcttttcgc gctggtgctg aagattctct actttcgtca acgccgttac
     5101 tacatcgatc acctcgtcta cgccctgcac attcactcct ttttttatat cgcgattctc
     5161 ttgaccgttt tcagcgcgat cggggcgaaa cactgggcac cggcgctgga gacgccgctc
     5221 ttgtggattc tctggcttgc gatcgtggcg caagttttcc tctcgatccg gcaggtctat
     5281 cgccagggct ggtccatgac gattttcaag ttcttcctcg gcggtttctt ctatctgttt
     5341 gttctgatcg ttgcgcttgc agctacggcg gtcgcgacaa ttgctctgct ctaggaagta
     5401 atttcctcca atcatgaaag cgctcttcct tcgctcgccc tccaggggga tgcacaccgg
     5461 cgcgcgctga ttcgccgcgg atcagctgag cagggctctt gccttggcga gggccgcttc
     5521 gattttggtc gcatcagtgc cgccgccctg cgccagttcc gggcgcccgc cgcccttgcc
     5581 cccgatgagt ggcgccgttt cctgaatcaa cttgcctgct tggaatcgtg cggtgagttc
     5641 ttttgggatg gccgcgatca acgcgacccg accatctttt tctccggcca gaaaaatagg
     5701 tccttcaaac ctggttcgga gcgcgtcggc caccgcctgg agcaacttgc cgtcggcgtc
     5761 cggca
//
LOCUS       DATMNS010000130         3103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2330878, whole genome shotgun sequence.
ACCESSION   DATMNS010000130 DATMNS010000000
VERSION     DATMNS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2330878"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /gene="thrC"
                     /locus_tag="VNW28_04765"
     CDS             complement(<1..953)
                     /gene="thrC"
                     /locus_tag="VNW28_04765"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXA09269.1"
                     /translation="MNENPQPEQAAARGRGVIACYAAWLPVTAATLVVSLGEGNTPLI
                     YSPRLSAAVGRGCEVFVKYEGLNPSGSFKDRGMTVAVSKALERGAQTLICASTGNTSA
                     SAAAYAARAGLRCAVVLPAGKIASGKMVQAFVYGARVVAIDGNFDQALEIVRALGERD
                     DFAIVNSINPDRIAGQKTAAFEIVDELGDAPDLHLLPVGNAGNITACWAGYQEYRAAG
                     KATRPPRMIGFQAAGSAPIYRGEIVPKPETVATAIRIGNPASWAQATCAIEESRGVID
                     IVTHEEILEAQRWLASAEGIFVEPASAAPIAGLLRAAAAGHA"
     gene            complement(971..2761)
                     /locus_tag="VNW28_04770"
     CDS             complement(971..2761)
                     /locus_tag="VNW28_04770"
                     /inference="COORDINATES: protein motif:HMM:NF002505.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClcB-like voltage-gated chloride channel
                     protein"
                     /protein_id="HXA09270.1"
                     /translation="MSTDHAPGGAPPLRLGFLRKLKWWVWFTEIIRPSDLQGTLAWAG
                     IIGFCGALTSIGFRYATSLLHKLLTRSNEPGLAESFIALPWWARLTIPALGGLLAGTV
                     LFVGMRWHGGVTTADYMEAVALGDGKISTRRSLVKCLSAMFTIASGGSIGREGPLVQL
                     SSLVASIAGRIRHWSTPRLRLLVGCGAAAGIASAYNAPIAGALFVAEIVLGSVAMEIF
                     GPLVFASVIATLTVRGFLGPDPIYAIPHFQLHSNWEIVPHLFLGLFAGLVAPWFLRLL
                     HASERLAGRVPVPVFLKMFVGGLIVGALAIFHPEVCGNGYSAVNTILAGHWAWQLVAV
                     ILIFKVLATAATFGSGAVGGVFTPTLFVGASLGYLFGIAAGHFPGGLTTNPSAFALVG
                     MGAFLAATTHAPIMAIIMIFELTLDYQIILPLMLACVVGYYTSVSIEKRSIYSESLKR
                     KGAGDYRRQLAELHVRDLLKPDPLTVSPTARFAEIGEKFIANRFNYLYVTEGQRFVGA
                     ISLHDIKNYLNAPELAELVIAGDILREDFPTIPPSATLGGALERFARHQGERLPVVSE
                     HEGHRLVGSLSKTDVILALAGSARQTVSAV"
BASE COUNT          627 a          994 c          980 g          502 t
ORIGIN      
        1 gcgtgtcccg ccgcggcggc acgcaagagc ccggcgattg gagctgcgct cgcgggctcg
       61 acgaagattc cttccgcgct ggccagccag cgttgtgcct cgagaatctc ctcatgggtc
      121 acgatgtcga tcacaccgcg cgattcttcg atggcacacg tcgcctgcgc ccagctcgcg
      181 ggattcccga tccggatcgc agtggcgacg gtctccggtt tggggacgat ctcgccgcga
      241 taaatcggcg cggaccctgc cgcctggaaa ccgatcatcc gcggaggtcg cgtggccttt
      301 ccggccgcgc ggtattcctg ataccccgcc cagcaggcgg tgatgttgcc ggcgttgccg
      361 accggcaaca gatgaagatc cggcgcgtcg cccaactcgt ccacgatctc gaaggcggcc
      421 gttttttgcc ccgcgatgcg atcgggattg atcgagttga cgattgcgaa atcatcccgt
      481 tccccgagtg ccctaacgat ctccagtgcc tggtcaaaat tgccatcgat cgcgacgacc
      541 cgggcgccat agacgaacgc ctggaccatt ttcccgctcg caattttgcc cgcgggcagg
      601 acgaccgcgc agcgcaggcc ggcccgcgcc gcgtaggcgg cggcggaagc ggaagtgttg
      661 ccggtcgagg cgcagatgag cgtctgcgcg ccgcgctcca gcgccttgga gaccgcgacc
      721 gtcatgccgc ggtccttaaa cgagccgctt ggattgagtc cttcgtattt cacgaacacc
      781 tcgcacccgc ggccgaccgc ggcgctcagg cgcggggagt aaatcagcgg agtatttccc
      841 tcgcctaacg agacaacgag agtcgcggcg gtcaccggaa gccaagccgc gtaacaagcg
      901 atcactcccc tgccccgcgc cgccgcctgc tccggctgag ggttctcgtt cattcgttag
      961 gcgccctttt tcacaccgcg gagacggtct gccgggcact gccggcgaga gccagaatga
     1021 catcggtttt cgagagactg ccgaccagcc gatgcccctc gtgttcgctc accaccggca
     1081 ggcgttcacc ctgatggcgg gcgaagcgct cgagcgctcc ccccagggtg gcggagggcg
     1141 gaatggtcgg aaaatcctcg cgcaggatgt cgccggcgat gaccagctcg gccagttccg
     1201 gcgcattcag gtaattcttg atgtcgtgca gggagatggc gccgacgaaa cgctgacctt
     1261 ccgtgacgta aagatagttg aatcgattcg cgatgaattt ttctccgatc tcggcgaagc
     1321 gcgcggtcgg cgaaacggtc aacggatcgg gcttaagcaa atcgcgcaca tggagttccg
     1381 ccagttgccg gcggtaatcg cccgcgccct tgcgcttgag cgactcgctg tagatcgagc
     1441 gcttctcgat gctgaccgac gtataataac cgaccacgca ggccagcatg agcggcagaa
     1501 tgatttgata atccagcgtc agctcgaaga tcatgatgat ggccatgatc ggcgcgtgcg
     1561 tcgtggccgc gaggaaggcg cccatcccga cgagcgcgaa ggcactcggg ttagtcgtta
     1621 ggccgccggg gaaatgaccg gcggcgatcc cgaacaaata accgagactc gcgccgacga
     1681 agagcgtcgg cgtaaagacg ccgccgactg cgccggagcc gaaagtcgcg gcggtggcga
     1741 gcaccttgaa aatcaggatt acggcgacga gctgccaggc ccaatggccg gcgagaatcg
     1801 tgttgaccgc gctgtagccg ttgccgcaga cctccggatg aaaaatggcg agcgcgccga
     1861 cgatcaatcc gccgacaaac atcttgagaa aaaccggaac gggcacgcgg ccggcaagac
     1921 gttcgctcgc gtggagcagc cgaagaaacc acggcgccac caggccggcg aaaagaccga
     1981 ggaaaagatg cggcacgatt tcccagttcg agtgcagttg gaaatgcggg atcgcgtaga
     2041 tcgggtcggg cccgagaaag ccgcgcaccg tgagggtcgc gataaccgac gcgaaaacga
     2101 gcggcccgaa aatttccatc gcaaccgagc cgagcacgat ctccgcgaca aagagcgcgc
     2161 ccgcgatcgg cgcgttatag gccgacgcga ttccggcggc cgcgccgcag ccgaccagca
     2221 gtcgaagccg cggggtcgac cagtgccgta tgcgaccggc aatcgaagcg acgagcgacg
     2281 aaagctggac gagcggacct tcccgcccga tcgaaccgcc cgaggcgatg gtgaacatgg
     2341 ccgagagaca tttcaccaag ctccggcggg tggagatttt tccatccccg agcgcgaccg
     2401 cctccatata atcggcggtc gtcacgcccc cgtgccagcg catgccgaca aagagtaccg
     2461 tgccggcgag caatccgccg agcgcgggaa tcgttaggcg ggcccaccag ggaagagcga
     2521 tgaagctttc cgccaaaccc ggctcattgc ttcgggtgag cagcttgtgc agcaggctcg
     2581 ttgcgtaacg aaaaccgatc gaagtgagcg cgccgcagaa tccgatgatc cccgcccagg
     2641 ccagcgtgcc ctgcaggtcg ctcggtcgaa tgatttcggt aaaccaaacc caccacttca
     2701 actttcgcag aaagccgagg cggagtggcg gcgcgccgcc gggcgcatgg tcggtcgaca
     2761 cgcctacgac gtgagcgcat cccgcgcgca cgtcaaatcg cgggacgctt gcagatcacg
     2821 cgattgtttt tatccacctt tgcgacgcag ccgcccgata ccgggaaatt ccagaagccg
     2881 gcgaatgcgg tcgtcgcgcc gctatagact cggagggaca tgcttccgca tgtcctggac
     2941 gcgcggaagc gcgtccctct gggaacaacg gcagttgaga cttccgcgcg acgagtagag
     3001 aatggacgcg gcgctggttc gctctctcga ctaacgactc tcaactctca acttccgatg
     3061 gctctgagca aaaaagataa actccggctc ctcacgattc tgc
//
LOCUS       DATMNS010000131         7011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_189887, whole genome shotgun sequence.
ACCESSION   DATMNS010000131 DATMNS010000000
VERSION     DATMNS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189887"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VNW28_04775"
     CDS             <1..502
                     /locus_tag="VNW28_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HXA09271.1"
                     /translation="RFLARAGIKKLVLTNAAGSVNPDFEPGSWMMITDHLNLTGTMPL
                     LGQSDFVDLTAAYSPDWRGRLGAAARAMGLPLHEGVYAGMLGPQYETPAEVRILRLVG
                     ADAIGMSTVCETIQARALGLEVAGFSCLTNWAAGLNPGPLSHREVLAAGEASGAVFMR
                     LLGAAL"
     gene            complement(603..1178)
                     /locus_tag="VNW28_04780"
     CDS             complement(603..1178)
                     /locus_tag="VNW28_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXA09272.1"
                     /translation="MTGPDLLASLAKLFGAKILEQIEFRGETTFVIAAGDIREIAKFC
                     KEELSFDYLLDISSVDHFGDEPRFEVVYELYSMTLSIHLRLKLRVPEDDPAVDTVSDI
                     WPTANWHEREIYDMMGLRFNGHPDLRRILMWDGYPYFPLRKEFPLEGKPSDMPDVAFS
                     SAAPMEGGPFVTQPSTATTKDREPRAHPPLD"
     gene            complement(1250..1807)
                     /gene="nuoB"
                     /locus_tag="VNW28_04785"
     CDS             complement(1250..1807)
                     /gene="nuoB"
                     /locus_tag="VNW28_04785"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF011390.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HXA09273.1"
                     /translation="MDAALASPNPQRLPAAYDSKIEGNVIFTQLDAAMNWMRKNSLWP
                     MPMGLACCAIELMATASARFDIARFGAEVMRFSPRQCDVMIVAGTVTYKMALAVKRIY
                     DQMPEPKWVIAMGACASSGGMYRSYAVLQGIDRLLPVDVYVSGCPPRPEALLEGLMKL
                     QNKISGERSFISQKRGLVEKIESLV"
     gene            complement(1964..5047)
                     /locus_tag="VNW28_04790"
     CDS             complement(1964..5047)
                     /locus_tag="VNW28_04790"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HXA09274.1"
                     /translation="MPPVPTVAVVRSVPEKKRGYFRNRIERFLTRDNFHFELLLGTAV
                     GVLVIVVLAVSCVVWSFRNQERDRLRARTVEVLRVSSAVGNDIAALDNAYRGRLLTKD
                     GAYVETFNRLQSLFLKDSEELTRLIGDDVEQKKRVLQLRKISQNWLKNSSLAMIDELQ
                     TPLDAARAKEALGASALDEVREIMQQIQRAEQIVLNELVGEQEWTSQSTQVLNFISKM
                     ERAASEMQKEERGYLLTGDPAFVDSYKRASSDFYTFHGYLTVLVASKPAEVDQLTRIR
                     ERLESWISHSATPDIATKREGQDFSATARAGRSESLMNVVRRSMEQFEKEQMDIYQVR
                     SAAAARARILSTFGIDLVCVIAAGLMIASSSYSFLLYRRQLKKLEIADARIRSVIDHV
                     LDGMITIDEKGAVASMNPAAKRMFGYAANENFEDEFIQLVPKYFAREIDDSPTTCDWA
                     GLVLRTGGTVLALAQARAGASFTVEISLSEMIVDQQKFYVAMIRDITERQRFEEELAA
                     EKKSLAVTLASIGDGVITTDLAGRVVICNAAGATMTGWTASEAVGQPLKAVFDLSADG
                     GKKRKRPGGAGYRSEAEGILLNTPEQSTLTSREGVKRMIEQVASPIRDGKNELCGVVL
                     VFRDIAERQRDEAERRKADTLEQLGLLAGGIAHDFNNLLTAIIGNISLVTLLLPPDDQ
                     MVARLEDAKNASLRARDLAQQLLTFARGGAPIKKTASIAKLIEETVSFSLRGSHSRGE
                     LAIEPNLWSAEFDPGQISQVIANLVVNADQAMPDGGILHVACDNFTYKADATPVIPDL
                     PPGDYIRIGLRDEGVGIPEHCLKQIFDPYFTTKPKGNGLGLATTYSVIKNHNGLITVE
                     SEQHCGSTFTIFLPAAQHLLPAPVEPVRPASEAMSGSGRILVVDDEEAIRALVEFTLS
                     RLGYEVFAAETAVRGIELYREALGRGQRFDLVILDLTLPGGMGGKDALKKLIEIDPMV
                     NAVVSSGYAMDATMSRYEDFGFRGVIAKPYEAAELGRTVHEAIAASRNGYASAFA"
     gene            complement(5121..5894)
                     /gene="truA"
                     /locus_tag="VNW28_04795"
     CDS             complement(5121..5894)
                     /gene="truA"
                     /locus_tag="VNW28_04795"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HXA09275.1"
                     /translation="MPNDEEAPEAGAWRRLKLIVAYDGFGFSGWQSQAKGDTIQDRLE
                     AAFARVVGQKIRVHGAGRTDAGVHALGQCAHVDLPTKLSPAVLLAALNASLPPQIRLL
                     RCQFVPRTFHARFSARGKIYRYRLATTPVLSPFEFGRAWHVAGSLDDGLMRACAREFV
                     GRHDFAGFAANRGHPVASTIRTIRALRVRRTSSGTVMEFDGEGFLYKQVRLMAGAIVR
                     CGLGKTSVAEVRERLHGAALEPSRLVAPANGLTLVRVRY"
     gene            complement(5887..6324)
                     /gene="ruvX"
                     /locus_tag="VNW28_04800"
     CDS             complement(5887..6324)
                     /gene="ruvX"
                     /locus_tag="VNW28_04800"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXA09276.1"
                     /translation="MGIDYGRARIGIALSDELQMLAHPAETIVVARRADPVARVAAIV
                     LEKNVERIVVGLPRHMNGSVGESANEANGFAEKLRAVVQCEVCTWDERLSTAAAHRAL
                     RAAGKSTRQTRDYVDQVAAQMILQSYLDRRQPNQGGAEPEPFA"
     gene            complement(6356..>7011)
                     /locus_tag="VNW28_04805"
     CDS             complement(6356..>7011)
                     /locus_tag="VNW28_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXA09277.1"
                     /translation="LVTIGIKPTWACPGFGYIEQGAPAPLPGGSAPNDIFRVVRFREK
                     PNVELADVFVRKGNFRWNTGMFVWSVPTVLSEFNRHAPELADFISQVRAPGNWESALR
                     ERFSNLPIISFDYAIMEKADRVLMVEANFDWDDVGGWRAVSAYLAQDENKNAANCDLA
                     TLQASDNIVFSEGATTIALLGVHNLIIVRTDDSILVCNRRDAEKVKDLVAKLPPKLQ"
BASE COUNT         1343 a         2123 c         2157 g         1388 t
ORIGIN      
        1 gcgctttctc gcgcgcgcgg gaatcaaaaa actcgttctg acgaacgccg cggggtcggt
       61 caatccggat ttcgagccgg gctcgtggat gatgattacc gaccatctta acctgactgg
      121 gaccatgcca ttgttaggcc agagcgactt cgtcgacctg acggctgctt actcgccgga
      181 ttggcgcggc cgtctcgggg ccgcggcccg ggcgatgggc ctcccgctgc atgaaggggt
      241 ctatgccggc atgctcggtc cgcaatacga gacgcctgcc gaagtccgga tcctgcggct
      301 cgtgggggct gatgcgatcg ggatgtcaac ggtgtgcgaa acgatccagg cgcgcgcgct
      361 cgggctggag gtcgccggct tttcctgcct aacgaattgg gccgccgggt tgaacccggg
      421 gccgctctcg catcgtgagg tgctggccgc gggcgaagcg agtggcgccg tgttcatgcg
      481 cctgctgggc gccgcgctct agaactagtg caatgtatca gaagaagcga gaccgctctg
      541 gcagatcatg gacatgcgtc cggcctcacg tccgcgggcg ctggtctcgc ttcacgtgat
      601 acctaatcga gcggaggatg agcgcgtggc tcgcggtcct tcgtcgtcgc ggtgctcggc
      661 tgcgtgacga aggggccgcc ctccatcggc gcagcggaac taaaggcgac atccggcata
      721 tcactcggtt tcccttcgag agggaactct ttgcgcagcg gaaaataagg atagccatcc
      781 cacatcagga tccggcgcaa gtcgggatgg ccattgaaac gcagacccat catgtcgtaa
      841 atctcgcgct catgccagtt cgcggtcggc caaatatccg agacggtgtc gaccgcggga
      901 tcatcttccg gcacgcgtaa cttgagccgc agatgaatcg acagcgtcat ggagtagagc
      961 tcgtaaacga cttcgaagcg cggctcatcg ccgaaatgat cgacgctcga aatatcgagc
     1021 agataatcga acgaaagctc ttctttgcaa aacttggcga tctcgcggat gtcacccgcc
     1081 gcgatcacga aagtggtctc accgcgaaat tcgatctgct ccagaatctt tgctccaaag
     1141 agcttggcca gcgacgcgag tagatcaggg ccggtcattg gggacgtgac gagtccgccg
     1201 cggtggataa attgtgagtt acgagggaca agattgacag gcgaaatttt tagactaacg
     1261 actcgatttt ttcgaccagg ccgcgctttt gcgagatgaa ggagcgctca ccactgatct
     1321 tattttgtaa cttcatcagg ccctcgagca gcgcctccgg tcggggcggg cagcccgaaa
     1381 cgtaaacatc caccggcaag agtcggtcga tgccctgtag cacagcgtaa gagcgataca
     1441 tgccgccgct cgaggcgcag gcccccatcg cgatgaccca tttcggctcc ggcatctgat
     1501 cgtagatgcg cttcacagcg agcgccattt tataggtcac cgtgccggcc acgatcatca
     1561 cgtcgcattg ccgcggcgaa aagcgcatca cctcggcgcc aaagcgggcg atgtcgaaac
     1621 gcgccgacgc ggtggccatt aattcgatcg cacaacaggc caggcccatc ggcatcggcc
     1681 agagtgagtt cttgcgcatc cagttcatcg ccgcgtcgag ctgggtgaaa atgacgttgc
     1741 cctcgatctt ggaatcgtag gcggcgggga gcctttgggg attcgggctg gccagcgcgg
     1801 catccattct gggaaataag ctagggggcg gcggcggcgg cgcaagcctg gagaggcttc
     1861 gctccaggaa gcgccgggga aaccggcggc gacagaagct gccggagctt gccgcaatcg
     1921 ctcccgcgct tcgcagagcc aggcgcctac gcccgaggcg cgattaggcg aaagccgacg
     1981 cgtagccgtt gcggctcgcg gcgatggctt catgcaccgt gcggccaagc tccgccgctt
     2041 cgtagggctt tgcaatcaca ccgcggaaac caaagtcctc atagcgactc atggtcgcgt
     2101 ccatcgcata gccgctggag acgaccgcat tcaccattgg atcaatctcg atcaatttct
     2161 tgagcgcatc ttttccgccc atcccacccg gcagggtgag atcgagaatc accagatcga
     2221 agcgctggcc ccgcccgagc gcctcacggt aaagctctat cccacgcacc gcggtctccg
     2281 ccgcgaagac ttcgtaaccg agacgggaaa gcgtgaattc aaccagcgcc cgaatcgctt
     2341 cctcatcgtc caccaccagg attcggccgg agccggacat cgcttcactc gccggcctga
     2401 ctggttcgac tggcgcgggt aataggtgct gcgcggccgg gagaaagatg gtgaaggtgg
     2461 aaccgcaatg ctgctctgat tcgaccgtaa tcaggccgtt gtgattcttg atcacggaat
     2521 aggtcgtcgc cagcccgagg ccgttgcctt tgggtttggt ggtgaagtag ggatcgaaga
     2581 tttgcttcag gcagtgctcc ggaatcccga cgccttcgtc gcgcaagccg atccgaatgt
     2641 aatcccccgg cggcagatca gggatgactg gggtcgcatc cgccttgtag gtgaagttat
     2701 cacaagcgac gtgcaggatc ccgccatcgg gcatggcctg gtccgcattc accaccaggt
     2761 tggcgatgac ctggctgatt tgtcccggat caaattccgc cgaccagaga ttgggctcga
     2821 tcgccaattc gccccggctg tgtgaaccgc ggagcgagaa gctgaccgtt tcttcgatca
     2881 gcttggcgat ggaggccgtc ttcttaatcg gtgcgccgcc ccgggcgaag gtgagaagtt
     2941 gttgcgccag gtcgcgcgcc cggagggaag cattcttggc gtcttcgagt cgggcgacca
     3001 tttggtcgtc aggcgggagg aggagggtga cgagcgagat gttgccgatg atggcggtga
     3061 gcagattgtt gaaatcgtgc gcgatgccgc cggcgaggag accgagctgc tcgagcgtgt
     3121 cggctttgcg gcgctccgct tcgtcgcgct gccgttcggc gatgtcgcga aagacgagga
     3181 ccactccgca aagttcattt ttcccgtcgc ggatcgggga ggcgacttgc tcgatcatgc
     3241 gtttcactcc ctcgcgggaa gtgagagtcg attgttccgg cgtgttcagg aggatgcctt
     3301 cggcttcgct ccggtaaccg gcgcctccgg ggcgcttcct tttcttcccg ccgtcagccg
     3361 aaaggtcgaa gacggctttg agcggctgcc cgacggcttc gctggccgtc caaccggtca
     3421 tggttgcgcc ggcggcattg caaatcacca cccgtccggc gagatcggtc gtgatcacgc
     3481 cgtcgccgat cgaggcgagg gtcacagcga ggcttttctt ttcggcggcc aattcctcct
     3541 cgaagcgttg tcgctccgtg atgtcgcgga tcatcgcgac gtaaaacttt tgttggtcca
     3601 cgatcatttc gctgagcgag atttccaccg tgaaggacgc ccccgcgcgc gcctgagcga
     3661 gcgcgagaac cgttccaccg gtgcgcagga caaggcccgc ccaatcacag gtggtcggtg
     3721 aatcatcaat ctctcgcgcg aaatatttcg gcacgagttg gatgaattcg tcctcgaagt
     3781 tttcgttcgc ggcataacca aacattcgtt tcgcagccgg attcatcgaa gcgaccgctc
     3841 ccttctcatc gatcgtgatc atgccatcga ggacgtggtc gatgacggat cggatccggg
     3901 cgtcggcgat ttccagtttt ttgagctggc gccggtaaag gaggaaactg tagctgctgg
     3961 aggcaatcat cagcccggcg gcgatcacac aaaccagatc aatcccgaat gtgctcaaga
     4021 tgcgcgcccg agccgcggcc gcggagcgca cctggtaaat atccatttgc tccttctcga
     4081 actgctccat ggaacggcgc acgacattca tcagggattc gctccgtccc gcccgcgctg
     4141 tggcggagaa atcctggccc tctcttttgg tcgcgatgtc gggtgtggca ctgtggctaa
     4201 tccaactctc cagtctttcg cggatgcggg ttaactggtc cacctcggcg ggcttgctcg
     4261 ccaccagcac cgtcaggtag ccatggaagg tatagaaatc ggacgacgcc cgtttgtagg
     4321 aatcaacgaa agccggatcg ccggtcaaaa ggtagccgcg ctcttccttc tgcatctcgg
     4381 aggcggcgcg ttccatcttg gaaatgaaat tgagcacctg ggtggactgg ctcgtccatt
     4441 cctgctcgcc caccagttcg ttcaagacga tctgctccgc gcgctgaatc tgctgcatga
     4501 tttcgcgtac ttcgtcgagg gccgacgcgc caagtgcttc ctttgcgcgc gccgcgtcga
     4561 gcggtgtctg cagctcgtca atcatggcta atgaactatt ttttagccaa ttctgactga
     4621 tcttacgcag ttgaaggacg cgctttttct gctcgacatc gtctccgatg aggcgcgtga
     4681 gctcttcgct gtccttcaga aaaagcgatt gcaatcggtt gaaagtctca acgtaagccc
     4741 cgtctttggt cagcaaacgg ccgcggtagg cattatcgag ggccgcgatg tcgtttccga
     4801 ctgcactgct gaccctcaac acctcgaccg tgcgggcgcg aagacggtct cgctcctgat
     4861 tgcggaacga ccaaaccacg caggacaccg cgaggaccac gatcacgagg acaccgaccg
     4921 ccgtgccaag gagtagttcg aaatggaaat tatcccgagt caggaatcgt tcgatccgat
     4981 tgcggaagta accgcgcttt ttctctggaa cgctcctgac gacggcaacg gttggcacag
     5041 gaggcatata cgggcatcat ccaaaagcaa ttcttctgcc atgtggcagg atggatattc
     5101 tcgcctttta actgcgggtc ttagtagcgc acgcgaacga gggtcaggcc gttcgcgggt
     5161 gcgacgaggc gggagggttc caaggcggcg ccgtgaagtc gctcccgcac ttcagcgacg
     5221 ctcgttttgc ccaggccgca gcggacgatg gctcccgcca ttagtcgcac ctgcttgtag
     5281 agaaaaccct caccgtcgaa ctccatgact gtgcccgaag aggtgcggcg cactcgcaaa
     5341 gcgcgaatcg tccggatagt cgatgcgacc ggatgcccac gattcgcggc gaaaccggca
     5401 aaatcatggc ggccgacaaa ttccctcgcg caggctcgca ttaatccgtc atcgagcgaa
     5461 cccgccacat gccacgcgcg gccgaattca aagggcgaaa ggaccggggt ggtggcaagg
     5521 cgatagcggt agatctttcc gcgggctgag aaccgggcat gaaacgtccg cggcacaaat
     5581 tgacagcgga ggaggcgaat ctgcggcggg agcgacgcgt ttaaggcggc gaggaggacc
     5641 gcgggcgaga gttttgtggg cagatcaacg tgcgcgcatt gcccgagcgc atgcactccg
     5701 gcgtcggtcc gtccggcgcc gtgcacgcga attttttgcc cgaccacccg ggcgaaggcc
     5761 gcctcgaggc gatcctggat ggtgtcgccc tttgcctggc tttgccagcc ggagaaaccg
     5821 aagccgtcgt aggcgacaat cagtttcaac cgccgccacg ccccggcctc gggcgcttct
     5881 tcgtcgttag gcaaaaggct ccggctccgc gccgccctgg tttggctgcc ggcgatcgag
     5941 atagctttgc aggatcatct gcgcggccac ctgatcgaca taatcgcgag tctggcgggt
     6001 cgatttcccc gccgcgcgca gggcacgatg ggcggccgcg gtcgagagcc gctcgtccca
     6061 ggtgcagacc tcgcactgga ccaccgccct tagtttttcg gcgaacccgt tcgcttcgtt
     6121 cgcggactcg ccgacgcttc cgttcatgtg ccggggcagt cccacgacaa tccgttcgac
     6181 atttttctcg agcacgatgg ccgcgacccg cgccaccgga tcggccctcc tcgccaccac
     6241 gatggtctcg gccggatggg ccagcatttg cagttcgtcg gagagcgcga tgccgatccg
     6301 cgccctcccg tagtcgattc ccatcaatct tttcattcgt taggcaggcg cccgctcatt
     6361 gcagcttcgg cggaagtttg gccaccaaat ccttgacctt ttccgcgtcg cgccggttgc
     6421 agaccaaaat ggagtcatcc gtccgcacga tgatgagatt gtgcacgccg aggagagcga
     6481 tcgtggtcgc cccttcgctg aagacaatgt tgtcgctcgc ctgcaaagtg gcgagatcgc
     6541 aattggcggc gttcttgttt tcgtcctgcg cgaggtaggc cgagacggcg cgccagccgc
     6601 cgacgtcgtc ccagtcgaaa ttcgcctcca ccatcaggac gcgatcagcc ttctccatga
     6661 ttgcgtaatc gaaggagatg atcggcaggt tgctgaaacg ttcccgtaaa gcggactccc
     6721 agttgcccgg cgcgcgaacc tgggaaatga aatcagccag ctccggcgcg tggcgattga
     6781 actcgctcag gacggtcggc acggaccaga cgaacattcc agtgttccag cggaaatttc
     6841 ctttccgcac gaagacgtcc gccagctcga cgttcggttt ttcgcgaaaa cgaaccaccc
     6901 gaaagatgtc gttaggcgcg gagccgcccg ggagcggggc gggcgctccc tgctcgatgt
     6961 agccgaaccc cgggcacgcc caggtcggct tgattccgat cgtaacgagc g
//
LOCUS       DATMNS010000132         6060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2331307, whole genome shotgun sequence.
ACCESSION   DATMNS010000132 DATMNS010000000
VERSION     DATMNS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6060
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2331307"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..2790
                     /locus_tag="VNW28_04810"
     CDS             148..2790
                     /locus_tag="VNW28_04810"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HXA09278.1"
                     /translation="MRHFASAFLLFFVLSSRVTAETKFSFATTPGKLPKTVLPVEYAI
                     RIVPDVEKLTFTGSETVKLEVQEAVTKIVLNALEMEISAASVDGQPLPKKAIALNEAE
                     QTLTLTLPDELATGEHQLALKFSGKINAQGQGLFYARYQEQGTNDKKLMLGTQFEATD
                     ARRMFPCWDEPSFRARFQLTAVVPKDFTAVSNMPVESEQKVEGGNEVRFAFSPPMSSY
                     LVVLVAGELDSIEAEQDGVKLRVVATKGKAEMGRYALESEARILHYYNEYFGVPYPLP
                     KLDLIAIPGGFGGAMENWGGITYYESVLLFDPQNSSAGTKQDIFAVIAHEMAHQWFGD
                     LVTMAWWDNLWLNEGFASWMGSKCTDHFNPEWNEWLRRNVSRDPSRRIGFPKDTAMQS
                     DARSTTHPVQQPIATEAEAGSAFDEITYRKGQSIIRMIESFLGEDVFRSGIRKYIATH
                     QYSNTTTADLWQALTEVSGKPVGEIAPGWTEQPGFPLVKVARDGNTIKLTQERFTVHF
                     PNPPALQWQIPLTYVTQDQPTTAGFLLRDPAADLPNELPGNRAIKLNVEDAGYYRVEY
                     DDTSWELLIAQLGNLSEADRVNLLIDAWALVEANRKPLSHYLGLVNQLMKDDQLAVYD
                     QVIDTFTFINRLLAGDPARPRFQQTARTVLRPAFDRVGWEAKPGEPSARAFLRASLIR
                     GLGLLNDSDIIAGCRSRFDRFLSDPAAVGPDLRPTIFAVVGRYADSQAWEKLHQLGLK
                     TTSAEERQNYYEALAGALDPRLVKRTIPLALTDELSTSRAALLLPFAARQSERPDLVW
                     AFAREHMKELLAKQDALGINGFAAGLFSYSSDVKDAATLQRYAKANLPSSAARAVQKT
                     VDEIGFRAEFKQRLLPQLHAWMDP"
     gene            complement(2801..3553)
                     /locus_tag="VNW28_04815"
     CDS             complement(2801..3553)
                     /locus_tag="VNW28_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXA09279.1"
                     /translation="MPTQGGSIRLFRIAGIEVFLHWSWFVVAVYEVQIWRSTFSSPVW
                     AAILYLGLFVLVTMHEFGHALACRQVGGQANRIVLWPLGGIAFVNPPPRAGAMLWSIA
                     AGPLVNVALVPILTLVLHTASRAGWESTNGDAYLVLLWLWRINLGLLLFNLLPIYPLD
                     GGQILRALLWFPLGQIQSLFVATAVGFVGGGALALGAIWKQSIWIGIMAFFLLSQAAA
                     GWRYAQYLKMEAAEELRGARDDSRLPEKKAAL"
     gene            complement(3587..3838)
                     /locus_tag="VNW28_04820"
     CDS             complement(3587..3838)
                     /locus_tag="VNW28_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09280.1"
                     /translation="MSEDSAFVDTDHLRECAALIVVAGLVDKPVIAVPTSAGYGASFG
                     GLAAPLGMLNSCGSGVTVVNIDNDFGAGYVAATINRLLG"
     gene            complement(3867..4352)
                     /locus_tag="VNW28_04825"
     CDS             complement(3867..4352)
                     /locus_tag="VNW28_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXA09281.1"
                     /translation="MSDVVIEPAISEDLDELSSLLGELFSEESDFRPDKEKQLRGLRL
                     IFEQPNRGRVFVLRRDRSIVGMINLLFTISTAEGGFVILLEDLVVHREYRGHGYGSML
                     LDYAIEFARQKNFRRITLLTDRPELRSQSFFRKHGFYESPMLPMRLLLPAQTEQRDAA
                     I"
     gene            4492..4665
                     /gene="rpmH"
                     /locus_tag="VNW28_04830"
     CDS             4492..4665
                     /gene="rpmH"
                     /locus_tag="VNW28_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HXA09282.1"
                     /translation="MKRTLQRSKRTRKQQHGFRARMSTKGGRATLARRRQRGRKRLLP
                     KGVEVHFARHTQA"
     gene            complement(4672..5754)
                     /locus_tag="VNW28_04835"
     CDS             complement(4672..5754)
                     /locus_tag="VNW28_04835"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HXA09283.1"
                     /translation="MRRRLVVCALALACTAADWKIAEPGWPYEFPRDHYLHEKFKTEW
                     WYFTGNLTNESGRRFGYELTFFRQGIRPPDERAATTSRFVVDDLKFAHFTVTDAAGKA
                     FLFQQKASRGSFGNAGFANNDRIAWIESWSLKLNADGSFDLAADTGDAEVQLHLIPSK
                     PPIVHGAGGLSRKAAGAGHASHYYSITRLKTSGSLRARKDRFVVTGESWFDHEWATNQ
                     LAPGQAGWNWVSAQFDDDSELMLYQMRLTSGAIDPISSGTFVRSDGSSTALTSADFQM
                     TPETFWKSKATQANYPIRWRITVPQEKLEFTIQPVLSNQELVLDPLVYWEGAFDLAGR
                     RAGNPVRGCGYLELTGYAGRLRELSR"
     gene            complement(5751..>6060)
                     /locus_tag="VNW28_04840"
     CDS             complement(5751..>6060)
                     /locus_tag="VNW28_04840"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXA09284.1"
                     /translation="RELAVLRSIGASAAQLRRLLLWESGMIGLLASLLGLTSGLCLAL
                     VLTGVINRAFFGWTIQLAFPWGSLAWTPLWIVAAAVAAGWIPAWRAGRLNVAEAIRSE
                     "
BASE COUNT         1268 a         1906 c         1789 g         1097 t
ORIGIN      
        1 aatgcgaccc tgagcaaaag cagtccactc gtgatccgcg gtccgcaaat tggcgcggga
       61 caactgcttc tcctgcttgt cgtccagtag cgcgctggag tgaaggcgag tcgattgcgg
      121 agcaccgcgc gcagcgttaa atgccccatg cgtcatttcg cttccgcttt tctccttttc
      181 tttgttctct cctctcgggt gacggccgaa accaaattca gtttcgcaac cacgccagga
      241 aagctgccga agactgtgct gccggtcgag tacgcgattc ggattgtccc ggatgtcgag
      301 aaactcactt tcaccggttc ggaaaccgtc aaactcgagg tgcaggaagc ggtcaccaag
      361 atcgtgctca acgcactcga gatggagatc tcggcggcgt ccgtcgatgg ccaaccgctg
      421 cccaaaaagg cgatcgcttt gaacgaagcg gagcaaacgc tgacgctcac cttgcctgac
      481 gagttagcga cgggagagca tcaactggcg ctcaagttca gcggaaaaat caacgcgcaa
      541 gggcaggggc ttttctacgc ccgttatcag gagcagggga ccaacgacaa gaaactcatg
      601 cttgggacac agtttgaggc gaccgatgcg cggcggatgt tcccctgctg ggatgagccc
      661 agttttcgcg cacggtttca gctcaccgcg gtggtcccaa aagacttcac cgcagtctca
      721 aacatgcccg tggagagcga gcaaaaggtc gagggcggaa acgaggtgcg ctttgccttc
      781 tcgccgccga tgtcgagtta tctcgtcgtg ctcgtggccg gcgaactcga ttccatcgaa
      841 gccgaacagg acggcgtaaa actgcgcgtc gtcgcgacca agggcaaagc ggagatgggc
      901 cgctacgcgc tcgagagcga ggccaggatt ctccactact acaacgaata tttcggcgtc
      961 ccctatccgt tgcccaagct cgatctgatc gccatcccgg gcggcttcgg cggtgcgatg
     1021 gaaaactggg gcggcatcac ctattacgaa tcggtcctgc ttttcgatcc gcagaattcc
     1081 tcggctggaa cgaagcagga tatctttgcg gtcatcgcgc acgagatggc gcaccaatgg
     1141 tttggcgatc tcgtcacgat ggcatggtgg gacaacctct ggctgaacga gggcttcgcc
     1201 tcctggatgg gctcgaaatg caccgaccat ttcaatccgg aatggaacga atggctgcgc
     1261 cgcaatgtgt cgcgcgatcc gagccggagg atcggttttc ccaaagatac cgcgatgcaa
     1321 tccgacgcgc gctcgacgac gcatcccgtg cagcagccga tcgcaacgga agcggaagcg
     1381 ggcagcgcct tcgacgaaat cacctacagg aaaggccagt caatcatccg gatgatcgag
     1441 agttttctcg gggaggatgt tttccgcagc ggcatccgca agtacatcgc gacgcaccaa
     1501 tattcgaaca ccacgactgc ggatctctgg caggcgctga cggaagtctc gggtaagcca
     1561 gtgggggaaa tcgcgcccgg ttggacggag caacctggat ttccgctggt caaggtggcg
     1621 cgggacggaa acacgatcaa gctcacgcaa gagcgcttca cggtgcattt cccgaacccg
     1681 ccggcccttc agtggcagat tccgctcacc tacgtaaccc aggaccaacc tacgaccgcc
     1741 ggctttcttc tgcgcgaccc ggcggcggat ttgcctaacg agttgccggg caatcgcgcg
     1801 atcaagctca acgtggagga tgctggttat tatcgcgtcg agtacgacga cacctcctgg
     1861 gaattgctca ttgcgcagtt gggaaatctt tcggaagccg accgggtaaa tctgctgatc
     1921 gatgcctggg cattggtcga ggcaaaccgc aagccgctct cgcattacct cggtctcgtc
     1981 aatcagctca tgaaggatga tcagttggca gtctacgatc aggtcatcga cactttcact
     2041 tttatcaacc ggctgctcgc cggcgacccg gctcgaccac gcttccagca aaccgcccgc
     2101 accgttttgc gccccgcctt cgatcgcgtc ggctgggagg cgaaaccggg cgaaccatcg
     2161 gcgcgtgcgt ttctgcgcgc ttcgttgatt cgtggtctcg gcctgctgaa cgattccgac
     2221 atcatcgcgg ggtgccgctc gcgtttcgat cggttccttt ccgatcccgc ggcggtggga
     2281 cctgatttgc gtccgaccat cttcgcagtc gtcggtcgtt acgccgactc ccaggcgtgg
     2341 gaaaaactgc accagctcgg tttgaaaacg acgagcgccg aagagagaca aaactattac
     2401 gaggcgctcg ccggcgcgct tgatcctagg ctcgtcaagc ggacgattcc actcgcgctc
     2461 accgatgagc tatcgaccag ccgcgccgct ctgttgctcc cctttgcggc gcggcagagc
     2521 gagcgtccgg atctggtctg ggcgtttgcc cgggagcaca tgaaggagct cctcgcgaaa
     2581 caggatgcgc tgggcatcaa cggcttcgct gccggactgt ttagctactc ttccgacgtc
     2641 aaggatgctg cgacgctcca gcgctacgcg aaggcaaacc ttccttcatc cgccgccagg
     2701 gcagtgcaga aaacggtcga cgagatcggc ttccgcgcgg aattcaaaca acgcctgctc
     2761 ccgcagttgc acgcgtggat ggacccgtag agcgaggcgt ctacagcgcc gcctttttct
     2821 cgggcagacg cgagtcgtcc cgcgctccgc gcaactcttc agcggcttcc attttcaaat
     2881 actgcgcgta acgccagccg gctgcagcct ggctcagaag gaagaaggcc atgatcccaa
     2941 tccagatcga ttgcttccaa atcgccccga gcgcgagcgc gccgccgcca acgaaaccga
     3001 ctgcggtcgc aacgaacaag ctctgaattt ggcccaaagg aaaccaaagg agagcgcgca
     3061 aaatctggcc gccatcgagg ggataaattg ggagaagatt aaacagaagc aggccaaggt
     3121 tgatccgcca aagccaaagc agcacgaggt aggcgtcgcc attcgtggac tcccagccgg
     3181 cgcgcgaggc ggtatgcagc acaagggtca gaattggcac cagcgcaacg tttacgagcg
     3241 gaccggcggc gatgctccag agcatcgcgc ccgcccgcgg cggcggattc acgaaagcga
     3301 tcccgcccaa gggccagaga acaattcggt tcgcctggcc gccgacctga cggcaggcga
     3361 gagcgtggcc gaactcgtgc atggtgacca acacgaacaa acccaaataa aggatcgctg
     3421 cccaaaccgg cgaagagaag gtggagcgcc aaatctgcac ttcgtaaacg gcgacgacaa
     3481 accatgacca gtgcagaaac acttcgattc cggcgatccg gaacaatcga atcgaacctc
     3541 cttgtgtcgg catcgataaa gatagcgcgg gccggcgccc gccgcatcaa ccgagtaggc
     3601 gattgatcgt ggcggcaacg tagccggcgc cgaagtcgtt gtcgatgttc actaccgtga
     3661 ccccgctgcc gcaactgttc agcatcccga gaggcgcggc gagaccgccg aagctcgccc
     3721 cgtatcccgc gctggtggga acggcaataa ccggcttatc cacgaggccc gcgacaacga
     3781 tcagcgccgc gcactcccgc aggtgatcgg tgtcaacaaa ggcggagtcc tccgacatgg
     3841 gtcaacacgg ggcggtggca gtccgctcag atcgccgcgt cgcgctgctc ggtttgggcg
     3901 ggtagcaata agcgcatcgg cagcattggc gattcgtaaa agccgtgttt gcggaagaaa
     3961 ctctgcgaac gcagctccgg ccgatcggtc agtagcgtga tccgccggaa attcttctgc
     4021 cgggcaaact cgatcgcgta atccagcagc atggagccgt agccgtggcc gcgatactct
     4081 ctgtgcacca ccaggtcctc gagcaggatg acgaagccgc cttccgcagt gctaatcgtg
     4141 aaaagcaggt tgatcattcc cacgatcgag cggtcccggc ggaggacgaa gacccggccc
     4201 cgatttggct gctcgaaaat gaggcgcagt ccacggagtt gtttctcctt gtcggggcgg
     4261 aaatcgctct cttcactgaa taactcaccg agcaggctcg ataactcgtc caggtcctcc
     4321 gagatcgccg gttcgatgac cacgtcgctc atcgcagatg gttattccac aatcggccac
     4381 cggtggcaat gcggttgtcg gcgcccgctc ggctggcaac cggcgttgac aaacccatgc
     4441 acgcccctaa ggtaggccct ctttcaacgc ccggcagtgg gttttttctc tatgaagcgc
     4501 accttacagc gatccaagcg aacccggaaa cagcagcacg gtttccgcgc gcgcatgagc
     4561 accaagggcg gccgcgccac gctcgcccgg cgtcgtcagc gcggtcgcaa acggcttctc
     4621 cccaagggcg tggaagtgca tttcgcccgc cacacgcagg cctaggctcg cctaacgact
     4681 cagttctcgg agccggccgg cgtaaccggt cagctcgaga tagccacagc cgcgcaccgg
     4741 gtttccagcg cgcctcccgg ccagatcgaa cgcgccctcc cagtagacga gggggtcgag
     4801 gacgagttcc tggttcgaca agacgggctg gatcgtaaat tcgagtttct cttgcggcac
     4861 ggtgatccgc cagcggatcg gataattggc ctgcgtcgct ttgctcttcc aaaatgtctc
     4921 cggcgtcatc tggaaatcgg cactcgtgag agcggtagag ctgccatcag atcggacgaa
     4981 tgttccgctc gaaatcgggt cgattgcacc actcgttagg cgcatttggt aaagcatcag
     5041 ctcgctgtcg tcgtcgaatt gcgccgaaac ccagttccag ccagcctgcc cgggcgcgag
     5101 ctgattcgtc gcccactcat ggtcgaacca gctttccccc gtgacgacga aacgatcctt
     5161 tcgcgcgcgc aggctgccgg aagtcttgag ccgcgtgatc gagtaataat gcgacgcgtg
     5221 tcccgcgccg gctgcctttc ggctgagtcc accggcgccg tgaacaatcg ggggtttcga
     5281 cgggatgagg tggagttgca cctccgcgtc acccgtgtcg gcggcgagat cgaaggagcc
     5341 atcggcgttc agcttcaagc tccacgattc gatccaggcg atccgatcat tgtttgcgaa
     5401 acccgcgttc ccaaagctcc cgcgactcgc tttctgctga aacaggaacg ccttcccagc
     5461 cgcgtccgtg acggtgaaat gcgcgaactt caaatcatcg accacaaagc gcgaagtcgt
     5521 tgctgcgcgc tcgtccggcg gacgaattcc ctggcgaaaa aacgttagct catagccgaa
     5581 tcggcgtccg ctctcgttcg tgaggtttcc ggtgaaatac caccactcgg tcttgaactt
     5641 ttcgtgcaaa taatgatcac gcggaaattc gtagggccat cccggctccg cgattttcca
     5701 atccgccgcc gtgcatgcga gcgccagcgc gcagaccacc aggcgtctcc tcattcgctc
     5761 cggatggcct ccgccacatt cagccggccc gcccgccagg ccggaatcca gccggccgcc
     5821 accgccgccg cgacgatcca gagcggcgtc cacgccagtg acccccaggg gaaggcgagc
     5881 tggatcgtcc agccaaagaa agcgcgattt atcacccccg tgagcacgag cgcgaggcaa
     5941 agccccgagg tcaagccgag cagactcgcc agcaggccga tcatgccgct ttcccaaagc
     6001 aggagccgcc ggagctgcgc cgccgaagcg ccgatcgagc gcaagacggc cagctcgcgc
//
LOCUS       DATMNS010000133         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4032274, whole genome shotgun sequence.
ACCESSION   DATMNS010000133 DATMNS010000000
VERSION     DATMNS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4032274"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..1682)
                     /gene="purB"
                     /locus_tag="VNW28_04845"
     CDS             complement(387..1682)
                     /gene="purB"
                     /locus_tag="VNW28_04845"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXA09285.1"
                     /translation="MIDRYSRAEMRALWSEQRKFEIWLEIETLACEAMAAIGEIPPAD
                     AAEIRRRARFSIPEINEIEKRTHHDVIAFLENVAASVGPAARWIHQGLTSSDILDTTL
                     AVQLVAAGKILLQDIRELRRVTGEQARRYKMTPMIGRSHGIHAEPITFGLKLALMFDE
                     FGRAEERLTQTLERVRVGKLSGAVGTHAHLDPRIERQVCEKLGLRPAAISTQIIQRDR
                     HAEFCVTLALIASSIDRWATELRHLQRTEVLEVEEFFSADQKGSSSMPHKRNPITAER
                     LSGLARVVRGNALAALENVALWHERDISHSSAERIILPDSCTLLDYMLDKLRDLIERL
                     QVYPENMERNLGLTKGLYFSQSVLLALTRAGANRQTAYEAVQRAAMRTWKGDQSFAEN
                     AKQEPEITALLSPAEIDALCSLDVHFRYVDQTFRALGLE"
     gene            complement(1798..2016)
                     /locus_tag="VNW28_04850"
     CDS             complement(1798..2016)
                     /locus_tag="VNW28_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09286.1"
                     /translation="MAFNLLIKAEPHMKKNTQILKTRPPLSLGDLILAVSSCTKNTKE
                     TVAAVADLLGSGQVRVENNGRFSRARVC"
BASE COUNT          430 a          634 c          693 g          437 t
ORIGIN      
        1 cccgaaaacc gcgtcgctga agttttcgag gcgagtgacc tcgagaccgc gttggcgaaa
       61 gccgtcgctg atttcacgat cgtgtctggc gcgagcggcg cggaacatgg agcactttga
      121 taagcgagcc gggcgtgcgc gacaagcggt taacgagacg cggagatcgt tgctccggca
      181 ggtggactgg cgaaacgctc cgtaggctcg tgcggacgat tctgggccgg gtagcgctcg
      241 ccgcggcgag ctggttgcgg tgttccaccg caacggactt ttctttaagc gccaccattc
      301 tcatgtcgtt cttgcggcca ggaaagtgcg ttgccgcgag acgcgtcaac cagcacgcga
      361 gacacatgcg ctacccaggc aaagcgctac tccaacccga gcgcgcgaaa cgtttggtcc
      421 acgtagcgga agtggacgtc gagcgagcaa agcgcgtcga tttccgcggg cgaaagcagc
      481 gccgtgatct cgggctcctg tttcgcattc tccgcgaagg attggtcgcc cttccaggtc
      541 cgcatcgcgg cgcgctgcac ggcttcgtaa gcggtctggc gattcgcacc ggcgcgggtc
      601 aacgcgagaa ggacggattg actgaagtaa aggcctttcg tcaggccgag attgcgttcc
      661 atgttttcgg ggtagacctg gagccgctcg atcaggtcgc gcagcttgtc gagcatgtaa
      721 tcgagcagcg tgcacgaatc cggtagaatg atccgttccg cgctcgagtg gctgatgtcg
      781 cgttcgtgcc agagcgcgac gttttcaagt gcggcgaggg cgtttcccct aacgacacgg
      841 gcgagtccgc tcagccgctc ggccgtgatc gggttgcgct tgtgcggcat ggacgagctg
      901 cctttctggt cggcgctgaa aaattcctcc acttcgagga cctcggtccg ttgcagatgc
      961 cgcagctccg tcgcccagcg gtcgatgctc gaggcgatga gcgcgagcgt gacgcaaaat
     1021 tcggcgtgcc gatcccgctg gataatctgg gtggaaatcg ccgccggtcg cagcccaagt
     1081 ttttcgcaga cctgccgctc aatccgcggg tcgagatgcg cgtgggtgcc aacggcgccg
     1141 ctgagtttgc caactcgaac acgctccaag gtctgcgtga ggcgttcttc ggcacggcca
     1201 aattcgtcga acatgagcgc gagcttgagt ccgaaggtga tcggctccgc gtggatgccg
     1261 tggctccggc cgatcatcgg cgtcatcttg taacgccgcg cctgctcacc ggttacgcgg
     1321 cgcagttccc ggatgtcttg caacaggatt ttgcccgcgg cgacgagctg caccgccagc
     1381 gtggtatcga gaatgtcgga cgaggtgagg ccctgatgaa tccagcgcgc cgcggggccc
     1441 acgctggccg ccacgttttc gaggaacgcg atcacatcgt gatgggtgcg tttctcgatc
     1501 tcgttgattt ccggaatcga aaaccgcgcg cgccgccgga tttccgcggc gtcggccggc
     1561 ggaatttcgc cgatcgcggc catggcttcg caggccaggg tttcgatctc gagccagatc
     1621 tcgaacttcc gctgctcgga ccagagcgcg cgcatctcgg cgcgggaata acgatcaatc
     1681 accgtacgaa catagaaagc gagacgacgg cggcaaagcg attcgtccaa aaagaaacgt
     1741 cgccccggcg gattaactac cggggcgacg cttcaaacgc gattcgacgg aaatttttta
     1801 gcagacccga gcccggctga accggccgtt attctcgacg cgcacctggc cactgccgag
     1861 caggtcggcg acggcggcga cggtctcctt cgtattttta gtgcaggagc taacggccaa
     1921 aatcagatca ccgagtgaca gcggagggcg ggttttgagg atttgggtat tctttttcat
     1981 atgaggctct gcctttataa gcagattgaa tgccatttcc taaatataga cgggtttccc
     2041 ggctaaacgt tcaaaaaaag ccgaaacctg gcggagtttg agaacctgtt cctgacaaca
     2101 gtgtattaaa catgcacact atgcactcag tgagagcgat tattgcgccg tgggtcgcct
     2161 cgcggtaagg cgcagaacca atagatataa gact
//
LOCUS       DATMNS010000134         8117 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_542892, whole genome shotgun sequence.
ACCESSION   DATMNS010000134 DATMNS010000000
VERSION     DATMNS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8117
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542892"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VNW28_04855"
     CDS             complement(<1..491)
                     /locus_tag="VNW28_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXA09287.1"
                     /translation="MNIHEYQAKELLQKFGAATPGGKVATTAEEAEQIARALGTNDLV
                     VKAQVHAGGRGKGTFANGFKGGVHLVKSPAEAREVAGKMLGQTLVTHQTGPAGRVVNK
                     VLVAVSVEIEREIYFAVLLDRATAAPLIVASTEGGVEIETVAEKSPEKIMREPIDPLA
                     GLQP"
     gene            complement(926..1294)
                     /locus_tag="VNW28_04860"
     CDS             complement(926..1294)
                     /locus_tag="VNW28_04860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09288.1"
                     /translation="MKVAVALTFTAALATSPLALADEHEKADAAATKEVTGEIVDMMC
                     YVDHNAMGEKHAACAEKCVKSGGPVGILSDGKAYLVVGAHKPMNDELAPFAGKTVTLK
                     GKMATNGGVSMLENAEIVKK"
     gene            complement(1357..2529)
                     /gene="carA"
                     /locus_tag="VNW28_04865"
     CDS             complement(1357..2529)
                     /gene="carA"
                     /locus_tag="VNW28_04865"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003280874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXA09289.1"
                     /translation="MPQPASRPLKALLALEDGRLFEGESFGAPGTGVGEICFNTSMTG
                     YQEVLTDPSYRGQIVAMTYPLIGNYGTNEADAESRSPQVRGFVVEELSGIASSWRTEI
                     SLEEYLRRWKIPGVQGIDTRALTRHLRTRGAMKACLTTEEISAEEAVRRAVEGAGVIG
                     MDYVREVTTPAAYQWDPEDQMSPAWSTASSRKATLPPIRHRVVAYDYGIKENILRRLR
                     QNGFGVTVVPATTTAEEVLQLKPEGVFLSNGPGDPSVLDYAHQAVRDLMGRTPIFGIC
                     LGHQVLGFAFGGRTFKLKFGHRGGNQPVQDLKTGRVAITSQNHGFAVDPESLPADVEV
                     THINLNDGTVEGLRHRELPIFSVQYHPEAAPGPHDASYFFAEFARLIENCDGKTND"
     gene            complement(2906..5257)
                     /gene="pheT"
                     /locus_tag="VNW28_04870"
     CDS             complement(2906..5257)
                     /gene="pheT"
                     /locus_tag="VNW28_04870"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXA09290.1"
                     /translation="MKFSLNWLREFVELPNDTAQLADLLTLAGVEIERIEARGASFAQ
                     VVVAQITASAPHPNADRLSVCQVDDGSGRARQIVCGAKNYKVGDKVPLAQPGAVLTGD
                     LKIKASKLRGVESQGMLCSAKELGIAEDAAGLLILSPEAKIGAPISELFPNDTILDVE
                     ITPNRPDLLSHYGLAREIAALTQKPLRALEIAEPQTANGSAVQISAPHECPFYSARRI
                     EKITVGPSPEWLRARLESVGLRAINSIVDITNFVMLEIGQPLHAFDADKLQGGINVRL
                     AQPNEEFLALDGRTYQLDPKNLLIADAARAIAIGGVMGGEDTGVTATTRHVLLESAYF
                     SPSSIRRTARELNLPSDASYRFERGVDPGMILRASERAAQLIREIAGGEPARETIVAG
                     TLPAAPAGFSLRYARCDELLGASVAPAEADRILENFGLQKLGATDRQSTWGIPSYRSD
                     LRREVDLIEEIVRVFGIHRITGADRSRFTPASAADRRYDAEMALRGRLVARGFSEVRT
                     SALVGRQTLGSGFAKDAIELRNPLSEDHVALRPSLLPGLIAALERNVRAGAKSVRLFE
                     VGRVFLPPKGEEIRRLALLLCGAARGPAHWRDAPARELDLFDLKGALESIGLGEVAYR
                     RCESAGFALAMEVLTSDHRCGVAGQLASAQAARAAAAVFVAELALPNELESVDGARKF
                     REWQRFPSVSRDIALIAPEALTHGEILATIQSAREPLLAGVELFDLFGGQSAANIGAG
                     RKSLAYSLTYLDKNRTLTSDEVSAAHDRIRERLKSELGVELRE"
     gene            complement(5428..6447)
                     /gene="pheS"
                     /locus_tag="VNW28_04875"
     CDS             complement(5428..6447)
                     /gene="pheS"
                     /locus_tag="VNW28_04875"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HXA09291.1"
                     /translation="MMHPLQELRDAALAEIAAAADMRALEDVRVLYLGRNGSISAWGE
                     KMKTLSKEERPVVGKLLNEVRSAVTAALEESGGKLRATQEIGALAEIDLSLPGTPHEI
                     GALHPLTQMLDRAIGIFRRMGFALAAGPDVEDEWHCFDALNTAPDHPARNEQDTFYLS
                     DGRLLRTHTSTVQIRAMESAPPPIRVIAPGAAFRRDEVDATHSAQFHQIEGLYVDQRV
                     SVADLKGTLEFFLRELFGAETAVRFRPHYFPFTEPSFEIDVKSKALKSGEKWIEVCGC
                     GMVHPAVFEEMNRARRDAAYDPEKWTGFAFGLGMDRLAMILFDIPDIRLFAQNELRFL
                     EQFAG"
     gene            complement(6547..6939)
                     /gene="rplT"
                     /locus_tag="VNW28_04880"
     CDS             complement(6547..6939)
                     /gene="rplT"
                     /locus_tag="VNW28_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXA09292.1"
                     /translation="MPRATNAPASRERRKRVLDAAKGYRGRRSKLFRYAKDATMKGKY
                     WAYRDRKTRKRNFRMLWVQRLNAAVRAQGLTYSRFAEGLKAANIGLDRKILSDLAITD
                     ESAFKSIVEQVKSALEKKSNESKKKSAA"
     gene            7380..>8117
                     /locus_tag="VNW28_04885"
     CDS             7380..>8117
                     /locus_tag="VNW28_04885"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HXA09293.1"
                     /translation="MHWLRRYRLLILGAICLFWTSLVLLLHFTPGVPFLSAVWSGEQR
                     VEDFLESEGRKTATRDDFVLLGIDQSTLELPPLLPEELADNRALQLMTARPFPWSREV
                     YALLLDRLFAAGARLVLFDLSFDPPNDGDAAFRAALDRYRDKVVIGANIDWANMRQAV
                     VPNPDLIPPPQMADPRVGYVIFIKDPLDQKLRWVHFTLTERQLAGQAPYPGEEVFYAL
                     SARGLEQLGRGADVPRDHHARFFRFSAN"
BASE COUNT         1453 a         2595 c         2447 g         1622 t
ORIGIN      
        1 ggttgcaacc cggccagcgg atcgatcggt tcgcgcatga ttttctccgg cgatttctcg
       61 gccacggttt cgatttccac gccgccttcg gtgctcgcga cgatcaaagg cgccgcggtc
      121 gcgcgatcga gcaacacagc aaaataaatc tcccgctcga tctccacgga aaccgcgacc
      181 agcaccttgt tcaccacgcg cccagccgga ccggtctggt gggtgacgag cgtttggccg
      241 agcattttcc ctgcgacttc ccgcgcttcc gcgggtgatt tcacgaggtg cacgccacct
      301 ttgaaaccgt tcgcgaatgt cccctttccg cgccctccgg catgcacctg cgctttcacg
      361 accagatcgt tcgtgccgag tgcgcgcgcg atttgttccg cttcctctgc cgtggtcgca
      421 accttcccgc ccggggtcgc agcaccgaac ttttgtagta attccttcgc ctgatattcg
      481 tgaatgttca tgcgcgggac ggttggacag acgagcaatt agctcgcgaa acgcaggcca
      541 gcaacggaat tttccccgtc ggattcgccg ctcacggcgg ccgggccgcc gagagacggg
      601 tcatccctgc ttgccgctga ccgcccctca cggttaaaca atttcagtca tcggagattg
      661 gaccgggcgg gatgaagacg cacctggcgt tccagccgac gaaaagactc agggatcacc
      721 gtcgaaacga aaggcggcga tcataccgca tcgagcacga gcgagagttc gtcgcggaga
      781 aagacgggcg attcgtcatc cccgtcccgg tgacggtgaa ataaggccgt ggagagcgcg
      841 cggcgctgag ttgtggccgc ggaaagaaga aacgcgcgct ccggggtagc cccaaagcgc
      901 ccgcttttcg ccttagccta acgaactatt tcttaacgat ctcggcgttc tccagcattg
      961 agacaccgcc gttggtcgcc atcttgcctt tcagggtcac ggtcttgccc gcgaacggcg
     1021 cgagctcatc attcatcggc ttgtgcgcgc cgacgacgag atacgctttg ccgtcgctca
     1081 gaatgccgac cggcccgccg ctcttgacgc acttttcggc gcaagcggcg tgtttctcac
     1141 ccatcgcgtt gtgatcgacg tagcacatca tgtcgacgat ctcgcccgtg acttctttcg
     1201 tcgccgcggc gtcggctttc tcatgctcgt cggccagcgc cagcggcgag gtggcgagcg
     1261 ccgccgtaaa ggtgagcgca accgccactt tcataaactt cggaactctg tcggtggtta
     1321 aatacatggc ctaacttccg accgaaccgc gccacgtcaa tcgttcgtct tgccgtcgca
     1381 gttttcgatc aggcgcgcga actcggcgaa gaaataactc gcgtcatgcg gcccgggcgc
     1441 ggcctcgggg tgatattgca cgctgaagat cggcagttcg cggtgccgca ggccttcgac
     1501 cgtaccgtcg ttcaggttga tgtgtgtcac ttccacgtcg gccggcaatg attcgggatc
     1561 aaccgcgaag ccgtgatttt gcgaagtgat cgcgactctg ccggttttca ggtcttgcac
     1621 cggctgattc ccgccgcgat ggccaaactt cagtttgaaa gtgcgcccgc cgaaggcgaa
     1681 cccgagcacc tgatgaccca ggcaaatgcc aaaaatgggc gtgcgcccca tcaagtcgcg
     1741 caccgcctga tgcgcataat cgaggaccga cggatcaccc ggcccgttcg aaagaaaaac
     1801 cccttccggc tttaactgga gaacttcttc cgccgtcgtc gtcgcgggga cgaccgtcac
     1861 gccaaaccca ttttggcgca gtcggcgcag gatgttttcc ttgatcccat agtcgtaagc
     1921 caccacccga tgccggatgg gcggcagggt ggctttcctg ctgctcgccg ttgaccaggc
     1981 tgggctcatc tggtcctccg gatcccattg gtaggcagca ggcgtggtga cctcgcgcac
     2041 gtaatccatc ccgatcacgc ccgcgccttc caccgcgcgc cggactgcct cctccgcgga
     2101 gatttcctcc gtcgttaggc aagccttcat cgcgccccgc gtgcgcaggt gccgggtgag
     2161 cgcgcgggtg tcgatcccct gcacgccggg aatcttccag cggcgcaaat attcctcgag
     2221 cgagatctcc gtccgccagc tgctcgcgat cccgctcagt tcctcgacca cgaatccgcg
     2281 cacctgcggc gaacggcttt cggcgtcggc ctcgttcgtg ccgtagttgc cgatgagcgg
     2341 ataggtcatc gcgacgatct ggccgcggta ggagggatcg gtcagcactt cctggtaacc
     2401 ggtcatcgaa gtgttgaagc aaatttctcc aacgccggtg ccgggggcgc cgaatgattc
     2461 gccctcgaaa agccgtccgt cctccagcgc gaggagggct ttgagcgggc gcgaagcggg
     2521 ctgcggcatc gtgtcgatgt agccgccaac cggccccgcg tccaaaaaaa agcgcccgaa
     2581 ccatcgctgg ttcgaacgcc tcaattaatt accccactga tgccgtttag gtggatatcc
     2641 cgttcccttc tggtaacggg cgggtgaccg ccgtcggagc gtctgacttc caattaaggc
     2701 ctgccatctt cccctaacga cctagccccc tctgctaaca acatcggtcc gggaatcaca
     2761 cctgtaatct cgaacaccgc agggtaaatt ttttatcccg aactcatttc ggggttcgaa
     2821 agttgtcgat agagagctga aagtagacac gagaaggtct ttgaaccttc aactctcaag
     2881 caacaactct cgaactttca aagaactact cgcgcagttc cacgcccagc tcgcttttca
     2941 atcgctcgcg aatccggtcg tgggccgcac tcacttcgtc agaagtcagt gttcggtttt
     3001 tgtcgaggta tgtcaatgaa taagcgaggg actttcgtcc cgcgccaata tttgcggcgc
     3061 tctggccgcc gaaaagatcg aagagttcaa ccccggcgag cagcggttcg cgggcacttt
     3121 ggatcgtcgc gagaatctcg ccatgggtca gcgcctcggg agcgatcagc gcgatatccc
     3181 ggctgaccga ggggaaacgt tgccattcgc gaaacttccg ggcgccgtct accgattcga
     3241 gttcgttagg caaagcgagc tcggcgacga aaaccgctgc cgcggccctg gcggcttgcg
     3301 ccgaggccag ctgaccagcc acgccgcaac gatggtcgct ggtcaaaact tccatcgcca
     3361 gcgcgaaccc ggcgctctcg caacgccggt aagcgacttc cccgaggccg atcgattcga
     3421 gcgctccttt gagatcaaag agatcgagct ccctcgccgg cgcgtcgcgc cagtgcgcgg
     3481 ggccccgcgc cgcgccacac agaagcagcg cgagccggcg aatctcttcg cccttcgggg
     3541 ggaggaagac gcggccaacc tcgaagagcc ggacgctctt cgcgcccgcc cgcacgtttc
     3601 tctccaaggc cgcgatcaag cccgggagca ggctcggccg gagggcgaca tgatcttcgc
     3661 ttaacggatt gcgcagctcg attgcgtctt tggcaaagcc cgagcctaac gtctgccgtc
     3721 cgaccagcgc agaggtgcgc acttccgaaa aaccccgcgc gacgaggcgc ccgcgcagcg
     3781 ccatctcagc atcgtagcgc cggtccgccg cggaagcggg tgtgaaccgg ctgcgatccg
     3841 cgcccgtgat ccggtgaatg ccgaagacac gcacgatctc ctcaatcagg tccacttcgc
     3901 ggcgcaaatc ggaccggtaa ctcggaatgc cccaggtcga ctgccggtcg gtcgcgccca
     3961 gtttttgcag gccgaaattt tcgagaattc gatccgcctc cgccggcgcg acggacgcac
     4021 cgagcagttc gtcgcaacgg gcgtagcgca gagaaaaccc ggctggcgcc gcaggcagtg
     4081 tgcccgcaac gatcgtctcg cgcgccggct cccctccggc gatttcgcgg attaattgcg
     4141 cggcgcgttc cgaggcgcga aggatcattc ccggatcaac cccgcgctca aaccggtaac
     4201 tggcgtcgct cggcagattc aactcgcgcg ccgtgcggcg aatgctcgac ggggaaaaat
     4261 aggcgctttc caaaagcacg tggcgcgtcg ttgcggtgac gccggtatct tcgccaccca
     4321 tcaccccgcc aatcgcgatc gcgcgcgcgg cgtcggcaat gagcaggttt ttcgggtcga
     4381 gctggtaggt ccggccgtcg agggcgagaa actcttcgtt aggctgagcg agtcgaacat
     4441 tgatcccacc ctgcaacttg tcggcatcga aggcgtgcag cggctgaccg atttccagca
     4501 tcacgaagtt ggtgatgtcg acgatgctat tgatcgcacg caggccgacc gactcgagcc
     4561 gggcgcgcag ccactcgggg ctagggccga ccgtgatctt ctcgatccgg cgcgcggaat
     4621 agaacgggca ctcgtgaggc gcgctgattt ggaccgcgct gccgttggcg gtttgcggtt
     4681 ccgcgatttc cagtgcccgc agcggttttt gcgtgagcgc cgcgatttcg cgcgcgaggc
     4741 catagtggct gaggagatcg gggcggttcg gcgtgatttc gacgtcgagg attgtgtcgt
     4801 tagggaaaag ctcgctgatc ggggcgccaa ttttcgcttc cggcgaaagg ataaggaggc
     4861 ccgcggcgtc ctcggcaatc ccgagctcct tcgcgctgca gagcatcccc tgcgattcca
     4921 ccccgcgcag tttgctcgct ttaattttca gatcgccggt cagaactgcg cccggctgcg
     4981 ccagcgggac tttgtccccc actttgtagt ttttcgcccc gcagacgatc tggcgcgcgc
     5041 ggccgctgcc atcgtcgact tggcaaacgc tgagtcggtc cgcattcgga tgcggcgccg
     5101 aagcggtaat ctgcgcgacc acgacctgcg cgaaacttgc gccgcgtgct tcgatcctct
     5161 cgatctcgac cccggcgagc gtgagcaaat cggcgagctg cgcggtgtcg ttaggcagct
     5221 cgacaaattc gcgcagccag ttaagggaaa atttcatttg ctaagcgtcg cgtgaagctc
     5281 tggggagcgc acgcgcctcg ggtgctgttt gcggcgcctc gccgcaaact ttccctgcgt
     5341 gtggagactc caaaaaagtt cgtgatggcg gggcgccatc accagcacac gaggcgcgtg
     5401 cgctccccgg agttctagcg cgccgtttca tcctgcgaat tgttccagga atcgcagctc
     5461 attctgcgca aagagccgaa tgtcgggaat gtcgaaaagg atcatggcga gccgatccat
     5521 tccgagcccg aaggcgaaac cagtccactt ttccggatcg taggccgcgt cacgccgggc
     5581 gcgattcatt tcctcgaaga cggccggatg gaccatcccg cagccgcaga cttcgatcca
     5641 tttttccccg cttttcagcg ctttggattt tacgtcgatc tcaaaactcg gttcggtgaa
     5701 gggaaaataa tgcggccgaa aacgcaccgc tgtctccgct ccgaaaagtt cgcgcagaaa
     5761 aaactcgagc gtgcctttga gatccgcgac gctcacccgc tgatcgacgt agagcccctc
     5821 aatctggtga aactgcgcgc tgtgcgtcgc gtccacttcg tcgcgccgga atgcggcgcc
     5881 gggtgcgatc acgcggatcg gcggcggcgc ggattccatc gcgcggatct gcacggttga
     5941 ggtgtgcgtt cggagcaacc ggccatcgga aagataaaaa gtgtcctgct cattgcgggc
     6001 ggggtgatcg ggcgcggtgt tgagcgcgtc gaaacaatgc cattcgtcct ccacgtctgg
     6061 cccggcggcg agcgcgaaac ccatccggcg aaagatgccg atcgcgcgat cgagcatttg
     6121 cgtgagcgga tggagtgcgc cgatttcgtg cggcgtgccg ggcagtgaaa ggtcgatctc
     6181 ggcgagcgca ccgatctcct gcgtcgcgcg caactttccg cccgactcct caagcgcggc
     6241 cgtgactgcg ctccgaactt cattcaatag cttgccgacc accggacgct cctcttttga
     6301 gagggtcttc attttctccc cccaggccga gatactcccg ttgcgcccga gatagaggac
     6361 gcgcacgtcc tcgagcgccc gcatgtcggc ggcggcggcg atctcagcca gcgccgcgtc
     6421 gcgcagctct tgcagaggat gcatcatgat gctgtcgccg cttcgctggg cgaagcgttg
     6481 accaggacta aaatctcggg cttcggcatg gcgtctcgct gcgtcgcccg cagggcgacg
     6541 gctacatcag gccgcgctct tcttcttcga ttcgttcgac ttcttctcca gcgcgctttt
     6601 tacctgctcg acgatgcttt tgaaagcgct ctcatccgtg atggcgaggt cggaaagaat
     6661 cttgcgatcg aggccgatgt tagccgcctt caggccttcc gcgaaacgac tgtaagtgag
     6721 accctgcgcg cggaccgcgg cgttgagtcg ttgcacccaa agcattcgga aattgcgttt
     6781 ccgcgtcttg cggtcgcggt aggcccagta ttttcccttc atcgtcgcgt ctttcgcgta
     6841 acggaagagt ttcgatcggc gcccgcgata gcccttggcg gcatcgagga cgcgtttgcg
     6901 gcgctcccgg ctggcgggcg cgttagtggc tcttggcatg ttgttgttcc cccgaatagt
     6961 ttcggaggat ctcctggagc gagctgttat ttcgttagga tctctccgac ctcactcgct
     7021 cgtgcggtcc cggcatcgcg gaaccaggtg gacagatccg acggcgcttc tttgcggaaa
     7081 cgtcgggctg ggaaaatagg cggagcgagc ggaatgacaa acgttttctg aggaaggcaa
     7141 ccttgaaggg cccagaatcg ttaggcaaaa aagccaggtt gccgatgggc ggccgtgctc
     7201 taccggcgcg tcgaatccca ccgtcggcag cggtggcggc gcggccggag gcgggcgcgc
     7261 tcgcgttcgg ccatctcgtt cgggtcgaac caaacaaaat ggcaatagcg acaggcgtcg
     7321 catcggcaca agctaaaatt ggaactggcc gctcggagaa gccttcctag aattgcccga
     7381 tgcattggct gcgccggtat cgcttgctca tcctcggcgc gatctgcctc ttctggacca
     7441 gtctggtcct gctcctgcat ttcacgccag gggtgccgtt tctctccgcc gtctggtcgg
     7501 gcgagcagcg cgttgaggat tttctggaaa gcgaaggtcg taagactgcg acgcgcgacg
     7561 atttcgttct tctcggaatc gaccagagca ccctcgagct gccgccgctc ctgcccgagg
     7621 aactggcgga caaccgcgcg ttgcagttga tgaccgcgcg ccctttccct tggtcccgcg
     7681 aagtctacgc tctgctcctc gaccggcttt tcgcggccgg cgcgcgtttg gtgcttttcg
     7741 acctctcctt cgacccgcct aacgacggcg atgcggcctt tcgcgccgcg ctcgaccgtt
     7801 accgcgacaa ggtcgtgatc ggcgcgaata tcgattgggc caacatgcgg caggcggtcg
     7861 tcccgaaccc ggacctcatt cctccgccgc agatggccga cccgcgcgtt ggttacgtca
     7921 ttttcatcaa ggaccccctc gatcaaaagc tgcgctgggt ccattttacc ctgaccgaac
     7981 gccagttggc gggtcaggcg ccctatccgg gcgaggaagt tttttatgcg ctctccgcgc
     8041 gcgggctgga acaacttggc cgcggagcgg acgtgccgcg cgatcatcat gcgcgttttt
     8101 tccgctttag cgcgaac
//
LOCUS       DATMNS010000135         3728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2698036, whole genome shotgun sequence.
ACCESSION   DATMNS010000135 DATMNS010000000
VERSION     DATMNS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2698036"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VNW28_04890"
     CDS             complement(<1..250)
                     /locus_tag="VNW28_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09294.1"
                     /translation="MALDHHPPMTDATPISRPLNRTVELLVRAKKRLAPSWQLENLPL
                     YVPAKKWIFNRLRQKEVTVHGFPFLLDPLDSLELSIFRS"
     gene            complement(240..1922)
                     /locus_tag="VNW28_04895"
     CDS             complement(240..1922)
                     /locus_tag="VNW28_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09295.1"
                     /translation="MISSSTGARRILLVLTGLALAGVALAWWLSGTFAGRADPRTAFS
                     AFYVIFARDEVLGLALVAAFCLGAALFIFREKDLPTPLADVPAAASHDGLVLVTIVAG
                     VFVVAALGTHFVCHDYALSADEYMADFQAQIFLRGKISAEIPPQWLDAVRVIKPTYVD
                     YFPATHAWKATYLPVYAALRAIFQGVYLQSLLNPFLAALTVLALYGAARNIWPNEKQN
                     AFVAVLLLGASAQFLAMAMTAYSMPAHLAFNTIWLWLYSRPDRRRFYLAPIVGALAIG
                     LHQPIVHALFVAPFLGRLAWERKWRAVSVFGFIYLLGCAGWYVWRARFSPPSSGAVVS
                     FFRLWNPKMLVIQPMDLLLVIGWSALATPLLAVLGFGRIFRERPIIQDAALSCALTFG
                     FYYFFCFDQGHGWGYRYFHGTLSCLVLVAVAGWGALAEKVGRRTARNFLAVAVAASVL
                     IVLPLRCYQAETFIRPFARASEAVHSMHVGVVGIDPRLGWYSADLIRNDPFLEHRPIM
                     AALFRLGPQEVAALQGAGKARFLTREEMAQFGLATTIHEHYRPDPFRLGYGP"
     gene            2026..3354
                     /locus_tag="VNW28_04900"
     CDS             2026..3354
                     /locus_tag="VNW28_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09296.1"
                     /translation="MELSADVSLPAIPEQTTNVLAGKRFLALSWSKALVVLTMATLLG
                     IGTFLRLYPTAGYQHLGTDERDYMVFVKQIQAAGIWNYDAVVQVYVERQYKLREAVVP
                     ATRVAFLAPAALCADLFHLRAFRALRVTAATAGVLLLFLCALFAYRLGGTIPMMGTTA
                     LLATGPLQIYLCQRALIDGYFAFWAVAALWLAWENLQHPRHTGWLVSYALCLTILVLT
                     KETAAFVVCALLGTLLLNRFLRLGTVTPQLLAATIAGPAIAVLLLATMVGGFGEWVRF
                     YQMFAAKSRTNFYCMAAQDGPWYRYAIDFALVTPAIVAFAIGGIFQLRKCDRAGVFMA
                     VFAALALIGLSCVKYGMSLRFAAFCDLPLTWLACSQVLALSRRFPGVRPALIAAGLFF
                     VLAAVGLNQYVRIFVKGGVYDPITNQLVAKLEMQKSPETVRAELAAATKH"
     gene            complement(3362..>3728)
                     /locus_tag="VNW28_04905"
     CDS             complement(3362..>3728)
                     /locus_tag="VNW28_04905"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA09297.1"
                     /translation="SPANAGINFALGNLCHAEGKREAAGNFYLAALRLDPANTGAYNN
                     LGVLALEESRWTLAINFLQHAVELSPNDAKTHFLLARADLKAGDVDAARREIGRAIQL
                     APSYPEFLTLRQEVENAGR"
BASE COUNT          780 a         1118 c         1156 g          674 t
ORIGIN      
        1 aactgcgaaa aatcgagagc tcaagcgaat cgagcgggtc gaggagaaag gggaaaccgt
       61 ggaccgtgac ttctttttgt cgcaaacgat tgaaaatcca cttcttggct gggacgtaga
      121 gcggcagatt ttcgagctgc caactcggcg ccagccgctt cttcgcacgc acgagcagct
      181 ccacggtgcg attcagcggc cgcgaaatgg gcgtcgcgtc ggtcattggc ggatggtggt
      241 caagggccat aacctaaccg aaacggatcg ggccgatagt gctcgtgaat ggtcgtcgcc
      301 agaccgaatt gcgccatttc ttcccgcgtc aaaaatcgtg ctttgcccgc gccttggagc
      361 gcggccactt cctgcggccc aagccggaac agcgccgcca tgatcggccg gtgctccaga
      421 aatggatcgt tgcgaatcaa atcggcggaa taccagccta gacgcggatc gatcccgacg
      481 acgccaacgt gcatcgagtg caccgcctcc gaggctcgcg cgaagggccg gatgaatgtc
      541 tccgcctggt agcagcgcag cggcagaacg atcaaaacgg aagcggccac ggcgaccgcg
      601 agaaaatttc tcgccgtccg ccggccgact ttttcggcga gcgctcccca gccggcgacg
      661 gccaccagga cgaggcagga aagcgtccca tggaaatagc ggtaacccca accatgaccc
      721 tgatcgaagc agaaaaaata ataaaaaccg aaggtcagag cgcagctaag ggccgcgtcc
      781 tgaatgatcg gccgctcccg aaaaatgcgg ccaaacccga ggacggcgag gagcggagtc
      841 gcgagcgcgc tccagccaat gacgagcaac aggtccatcg gctgaatgac gagcatcttc
      901 ggattccaaa ggcgaaaaaa cgaaacgacc gcgccgctcg aagggggcga aaacctggct
      961 cgccagacgt accatccggc gcagcctaac aaatagatga aaccgaacac tgagacggca
     1021 cgccatttcc gctcccacgc cagccggccg agaaagggcg cgacaaagag ggcatggaca
     1081 atcggttggt gcagcccgat ggcgagggcg cccacgatcg gcgccaggta gaagcggcgg
     1141 cgatccgggc gcgaatagag ccagagccaa atggtattga acgcgagatg cgcgggcatg
     1201 gagtaggcgg tcatcgccat ggccagaaac tgggccgagg cgcccaacag gagaacggcg
     1261 acgaaggcgt tttgcttttc gttaggccaa atgttgcggg ccgcaccgta aagagcgagc
     1321 acggtcagcg cggccagaaa aggattcaag agcgactgca ggtagacgcc ctggaaaatc
     1381 gcgcgcagcg ccgcgtagac cggcagataa gtcgccttcc aagcgtgtgt ggccggaaag
     1441 taatccacat aggtcggctt gatcacgcgc acggcatcga gccattgcgg tggaatttcg
     1501 gcactgattt ttccgcgcag gaaaatctgg gcctggaaat ccgccatgta ttcgtccgcg
     1561 ctcaaggcgt aatcgtggca gacgaaatgg gtcccgagcg cggcaacgac gaaaacgccc
     1621 gcgacgattg tcaccaagac caaaccgtcg tgcgacgctg cggctgggac atcggccagc
     1681 ggagttggca aatccttttc gcggaagatg aacaacgccg cgccgagaca aaaggccgca
     1741 accagcgcca aacccagcac ttcgtcccga gcaaaaatga cgtagaacgc gctgaaggca
     1801 gtccggggat cggcccgtcc ggcgaaggtg ccgctgagcc accaagccag cgcaacgccg
     1861 gccagtgcca ggccagttaa gaccaagaga atccggcgtg caccggtcga cgacgaaatc
     1921 atggtgggag aaggaagcct gcttcgtgag cagccaacgg gccagagaaa ggcgaagagt
     1981 cgtgcgagcg ggccgtctag cacgagactt gcggcatttg agcggatgga actctcggcc
     2041 gacgtgtctt tgcccgccat cccggaacag acaacgaatg ttctcgcggg aaaacgattc
     2101 ctggccctga gctggtccaa ggcgctcgtc gttctcacga tggcgactct gctcgggatc
     2161 ggaacattcc tccgccttta cccgacggcg ggttatcagc atctcggcac agacgaacgc
     2221 gactacatgg tctttgtgaa gcaaatccag gccgctggca tctggaatta cgatgccgtt
     2281 gtccaagtct atgtggagcg gcaatacaaa ctgcgcgaag cggtggttcc ggcaacgcgc
     2341 gtcgcgtttc tcgccccggc ggcgctctgt gcagatctct ttcacctgcg cgctttccgc
     2401 gcactgcgcg tgacagcggc aaccgctggt gtgcttctgc tctttctctg cgcacttttc
     2461 gcttatcgcc tcggcggaac gattcccatg atgggcacga ccgcgctcct cgccacgggg
     2521 ccgcttcaga tctatctctg ccagcgagcg ctgatcgatg gctacttcgc cttctgggcg
     2581 gtcgctgcgc tctggctggc ctgggaaaat ttgcagcatc cgcgccacac cggctggctg
     2641 gtctcctacg cgctttgcct aacgattttg gtgctgacca aggagacggc ggctttcgtc
     2701 gtctgcgcgc ttctcggaac gctcttgttg aatcgtttcc ttcgtctggg aaccgtcacg
     2761 ccgcagctcc tggccgcgac catcgcgggg ccggcgatcg ccgtcctgct cctcgccacg
     2821 atggtgggcg gcttcggcga atgggtgcgt ttctaccaga tgttcgccgc caaatcgcgg
     2881 acaaattttt actgcatggc ggcgcaggat ggaccctggt atcgctatgc gatcgacttt
     2941 gcgctcgtga cgcccgcgat cgtcgcattt gcgatcggcg ggatttttca actacggaaa
     3001 tgcgatcggg ccggagtgtt catggcggtg ttcgccgcct tggcgttgat cggcctgtcg
     3061 tgcgtgaaat acggaatgag ccttcgtttt gccgcgttct gcgaccttcc gctgacctgg
     3121 ctggcgtgct cgcaggtgct cgccctgagc cggcgatttc cgggcgtccg cccggcgctt
     3181 atcgcagctg gtttgttttt cgtgctcgcc gccgtcgggc ttaatcaata cgtccggata
     3241 ttcgtcaagg gcggggttta cgatccgatt acgaatcagc tcgtcgcgaa actcgagatg
     3301 cagaaatcgc ccgagaccgt ccgcgccgaa ctggccgcgg cgaccaaaca ttgattccaa
     3361 ttcagcgtcc cgcgttttca acttcctgcc gcaaggtaag aaactccgga tagcttggcg
     3421 cgagctgaat ggcgcgcccg atctcccgtc gcgcggcgtc gacatcgccc gctttcaggt
     3481 cggcacgggc gagaagaaaa tgcgtcttgg cgtcgttagg cgaaagctcg accgcgtgct
     3541 ggaggaagtt tatggccagc gtccatcgac tctcctcgag ggcgagaacg ccaaggttgt
     3601 tgtaggcgcc ggtattcgcc ggatcaaggc gcagtgcggc taggtagaaa ttacccgcgg
     3661 cctccctctt gccctcggcg tggcaaagat tgccgagcgc gaaattgatt cccgcgttcg
     3721 ccggggaa
//
LOCUS       DATMNS010000136         2171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1625299, whole genome shotgun sequence.
ACCESSION   DATMNS010000136 DATMNS010000000
VERSION     DATMNS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1625299"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1526
                     /locus_tag="VNW28_04910"
     CDS             <1..1526
                     /locus_tag="VNW28_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXA09298.1"
                     /translation="RQREMAVRAALGAGRQRLMRQLLTESVLLAFAGGTAGLLLAAWG
                     TRLLVSLLARNFDIPRLGDTHTDLWVLGFTLLVSAAAGILFGILPALGAASADVNEGL
                     RESSRSSTGNLRGQRIRSSLVVMETALALILLSGAGVLLKSLLVMRGTAPGFRTDNLL
                     AVEFSLPRIKFAKTPERLRFFTSVLNRVEGIPGVRSAALVADLPLGGGEDGLGFHIVG
                     RPDPSPSTSFQASFNIVSAGYFQTMGIPLHAGREFTEQDSVGTPGVVVVNDTAARRFW
                     PGENPLGKQIILDAAQPPLTVGGVAGDVRQRSLGVPPKPEIYLDYLQPGPDWPWLVLV
                     ARTTEQPAKLAGTVKAVAASVDQDVPVLRASTMDEILSSSLAQPSVYALLLGVFASLA
                     LALAAVGLYGIVSYAVTQRIHEMGIRMVLGAARGEILRLVLRRGMSLAFAGSLIGLGV
                     ALAARRVLAGMVHDAEASDPLAISAVTLLLLAVAFLASYLPARRAARVDPMVALRYE"
     gene            1543..>2171
                     /locus_tag="VNW28_04915"
     CDS             1543..>2171
                     /locus_tag="VNW28_04915"
                     /inference="COORDINATES: protein motif:HMM:NF038403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease prefix domain 1-containing protein"
                     /protein_id="HXA09299.1"
                     /translation="MHTFLSRLFSLFRKRRLNAELDDELRSHLQLLTEENIRRGMNSR
                     EAREAARREFGGVEQTKEAYREQRGLPFLDTLWQDLRFALRMLAKKPGFAAVAVLTLA
                     VGIGATSAVFSVVDRILFRSLPYPQDERLVSFGVMAPFDTREFMLGPDFVDWRGRQAP
                     FEPITAVEPGSADCDLTEQNPARLSCGQADAAFLPTFRVQPIVGRNFTR"
BASE COUNT          352 a          639 c          709 g          471 t
ORIGIN      
        1 cgcgacagag ggagatggcg gttcgagcgg cgttgggcgc aggtcgccag aggctgatgc
       61 gccagttgct gacggaaagc gttctgctcg cgttcgccgg cggcacagct ggcctgttgc
      121 ttgcggcctg gggcacgcgg ctgctggtat ccctgcttgc caggaatttt gacattcccc
      181 gcctcggcga tacgcatacc gacctgtggg tcctcggttt tactcttctc gtctccgcag
      241 cggccggcat tctgtttgga atcttgccgg cactgggcgc ggcttccgcg gacgtgaatg
      301 aaggattgcg cgaatcgtcg aggtcgtcga ccggcaacct gcgcgggcag cgcattcgct
      361 cgagcttggt ggtgatggaa accgcgctcg ccctgatctt gctctctgga gccggcgttc
      421 ttttgaagag ccttctcgtc atgcgcggca ctgcgcccgg ttttcgtacg gataacttac
      481 tggcggtgga gttttccctg ccgcggatca agttcgcgaa aaccccggaa cgcttgagat
      541 tttttacgag cgtcctgaat cgcgtcgaag gcatacccgg tgtgcgctcg gcggcgctgg
      601 tggcggattt gccgctcggc ggcggggaag acggtcttgg atttcacatt gtcggccgcc
      661 cagatccgtc accgagcacc agtttccagg cgagtttcaa tatcgtcagc gcaggatatt
      721 tccaaactat ggggataccg cttcatgccg ggcgcgaatt cacggaacaa gattccgtgg
      781 gaacgccggg cgtggtggtc gtgaatgaca cggcggcacg gcgcttctgg cccggggaaa
      841 atcccttggg gaaacagata atcctcgacg ccgcgcaacc gccgctcacg gtcggcggcg
      901 tggcgggaga cgtcaggcag cggagtctcg gcgttcctcc caaaccggaa atttacctgg
      961 attatttgca gcccggaccg gattggccct ggttggtcct ggtcgcgcgc acgaccgagc
     1021 aacccgcgaa gctcgccggc acggtcaagg ccgttgccgc ttccgtcgat caggatgtgc
     1081 ccgtactacg ggctagcaca atggacgaga ttctttcttc ttcgctagcc cagccgagtg
     1141 tgtacgcgct gcttctcgga gtgtttgctt cgctggcgct ggccctcgct gccgtcggcc
     1201 tctacggcat cgtgtcgtac gcggtgacgc agcgaatcca tgagatggga attcgcatgg
     1261 tgctgggcgc cgcacgcgga gagattttgc ggctggttct gcggcgcgga atgagcctgg
     1321 cgttcgccgg ctcgctgatt ggactcggcg tcgcgttggc tgcgaggcgc gtgctggccg
     1381 gcatggtgca cgacgcggaa gccagcgatc cgctggcgat ttcggcggta acgctcttgc
     1441 tgctggcggt ggcgttcctt gccagctatt tgccggcgcg ccgcgctgct cgggtcgatc
     1501 cgatggtcgc cttacgctac gagtgagaag gagttgaaac tgatgcacac ttttctatcg
     1561 cggctttttt ctctctttcg taagcggcgc ctgaacgcag aactggacga cgagcttcgt
     1621 tcccaccttc aactccttac ggaagaaaac attcgccgcg gcatgaactc gcgagaagcc
     1681 cgcgaagcgg cgcgtcgcga atttggcggc gtggagcaga ccaaggaagc gtatcgcgag
     1741 cagcgcgggc tgccatttct cgacacgctg tggcaagact tgcgtttcgc gctgcgcatg
     1801 ctggccaaga aacctggttt cgcggcggtg gccgttctga cacttgccgt cggtatcgga
     1861 gcaacttcgg cggtattcag cgtggtggac cgaattttgt ttcgcagctt gccctatccg
     1921 caagacgagc gcctggtttc ttttggagta atggcgccgt tcgacacccg cgaattcatg
     1981 ctcgggcctg attttgtgga ttggcgcggg cgacaagcgc cgtttgaacc gatcacggcc
     2041 gtcgagcccg gtagcgcgga ctgcgatctg accgagcaaa atccggcgcg gttaagctgc
     2101 gggcaagccg acgctgcctt cctgccgacg tttcgcgtcc aaccgattgt cggccgaaat
     2161 ttcacacgcg a
//
LOCUS       DATMNS010000137         3725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2962885, whole genome shotgun sequence.
ACCESSION   DATMNS010000137 DATMNS010000000
VERSION     DATMNS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3725
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2962885"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VNW28_04920"
     CDS             complement(<1..1174)
                     /locus_tag="VNW28_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXA09300.1"
                     /translation="MAELSKAYESQAVEAKWYARWLAEGFFQADPASPKPAYSIVIPP
                     PNVTGVLTLGHVLNNTIQDILARRARMLGKEVLWLPGTDHAGIATQTVVERQLRKEEG
                     KTRHDLGREEFVRRVWQWKEKHGGIIIEQLKRLGCSCDWSRERFTMDADYSRAVQRTF
                     VDLYQKGLIYRGRRMVNWDPVALTALSDEEVIPTPQKSALYYVRYELVEEPGRFIEVA
                     TTRPETIMADVGLAVHPDDDRYRPLLGKKAWRPLRRAPVPIVADEGIDPAFGTGVLKV
                     TPAHDKLDFEIGQRHNLPVIDVLHPDGTINCAEVPELNGLDRFVARKKAAEMLAESGL
                     LAKEEPHESTVGFSERAGVPIEPRLSEQWFLKYPRVEEARAVVGSASARDSPSEPGV"
     gene            1254..1616
                     /locus_tag="VNW28_04925"
     CDS             1254..1616
                     /locus_tag="VNW28_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXA09301.1"
                     /translation="MEKVNLAEKFAAFAEHWRPKIVGELNGQEVKVVKFQGVFPWHQH
                     ANEDELFLVWRGRMRIEFRDRIVELDPGEFLIVPHGIEHRTAADEETEVVLFEPAATR
                     NTGSAVESEFTAPDGARI"
     gene            1740..2855
                     /locus_tag="VNW28_04930"
     CDS             1740..2855
                     /locus_tag="VNW28_04930"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXA09302.1"
                     /translation="MRLPRIGLAFLLASAFISRASAEDWKTIHAEGRAYVSFANVAQF
                     YRFPRFTQASRSVSLRDDRRALLAQGGTSEFYINGVRFFSDYPLLAKPEENLISATDV
                     AKIIEPVLRPNKISGAKKIDTVVLDPGHGGGDSGASGPWGNEKSYALDVALTARENLL
                     RAGFKVEMTRSRDESVSLEDRVAFANRFPNAVFISIHFNSAEGGSGVETYALAPEGVP
                     SNASTESHPSSFDTQGYPGNPQDPANIALAAAVHGAILSRVSVFDRGVRHARFHVLRD
                     IKIPAVLVEAGFLNDRIEGAHIATPQYRQQLGLALAQAVTTYDRAVNFQSSGPTIVAA
                     STNLPPHTHSITDSLGDGAPDRVNPPPEPSAVVHASK"
     gene            2856..3704
                     /gene="murI"
                     /locus_tag="VNW28_04935"
     CDS             2856..3704
                     /gene="murI"
                     /locus_tag="VNW28_04935"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HXA09303.1"
                     /translation="MNPPARATGAPAREKPIGVFDSGIGGLTVVRALRELLSNETIFY
                     LGDTARVPYGGKSAATVERYSLEIASLLLEENCKAIVVACNTASALALPKLEATTPVP
                     VIGVIQPGAKAAVAATRNRHIGVIGTRATIKSGAYERAILALDPAVRVSARACPLFVP
                     LIEEGWLEDEITDRVVRQYLDPLVRDGIDTLVLGCTHYPLLREAIGRYLGDAVRLVDS
                     AQNCATAVRQLLEAEDLRATAVTAGKLSVALTDPPDAFLDVAQKALRLDIGTVQLRNV
                     IHPAKS"
BASE COUNT          699 a         1222 c         1109 g          695 t
ORIGIN      
        1 ccacgcccgg ttcactcggc gagtcgcgtg cgcttgccga gccaacgacc gcgcgcgctt
       61 cttcgacccg cggatatttc aaaaaccact gttcgctcag acgcggctcg atcggcacgc
      121 cggcgcgttc gctgaagccg accgtgctct cgtgcggttc ttctttcgcg agaagaccgc
      181 tctcggcgag catctcggcc gccttctttc gcgccacgaa acggtccagc ccgttgagct
      241 cgggcacctc ggcacaatta atcgtgccgt cggggtgcaa gacatcgatc accggcaggt
      301 tgtgccgctg gccgatctcg aaatcgagct tgtcgtgtgc cggggtcacc ttgaggacgc
      361 cggtgccgaa cgccgggtcg attccttcgt cggccacgat cggcaccggt gcgcggcgca
      421 gcggtcgcca ggctttcttg cctaacaatg gacggtaacg atcatcgtcc ggatgcaccg
      481 ccagacccac gtcggccatg atcgtttcgg gccgcgtcgt tgcgacctcg atgaatcgtc
      541 ccggttcctc gaccaattca tagcgcacat agtaaagcgc gcttttttgc ggagtcggga
      601 taacctcctc atccgagagc gcggtcagcg cgaccggatc ccagttcacc atccgtcggc
      661 cgcgatagat gagccctttc tggtagaggt cgacaaacgt gcgctgcacc gcgcgcgagt
      721 aatcggcatc catggtaaaa cgctcccggg accaatcgca ggaacagccc agccgtttga
      781 gctgctcgat gatgatgccg ccgtgttttt ccttccactg ccagacgcgc ctgacgaact
      841 cttcccgccc aaggtcgtga cgggtcttcc cttcctcctt gcgcaactgc cgctcaacca
      901 cggtctgggt cgcgattccg gcgtgatcgg ttcccgggag ccagagcact tctttgccta
      961 gcatgcgcgc gcgccgggcg agaatgtcct gaatggtgtt attcaagacg tgcccgagcg
     1021 tgagcacgcc ggtgacattc ggcggcggga taacaatcga atacgccggt ttcggcgatg
     1081 cgggatcagc ctggaaaaag ccctcggcca gccaccgggc gtaccatttc gcctcgacag
     1141 cctgcgattc ataggccttg gaaagttccg ccatgatggg gagcaatcat cgaagagcag
     1201 gcgacgattg caaaacgaaa actccggccg tcgctgtgct agattccagg ccgatggaaa
     1261 aggtgaatct cgcggaaaag ttcgcggctt ttgcggagca ttggcggccc aagatcgtcg
     1321 gcgaattgaa cggccaggaa gtgaaagtgg tgaaatttca gggagtgttc ccgtggcatc
     1381 agcacgcgaa cgaggacgaa ctgtttctcg tctggcgcgg ccggatgcgg atcgaattcc
     1441 gcgaccgaat cgtcgagctc gatccaggcg agtttctcat cgtcccgcac ggcatcgagc
     1501 accgcacggc ggccgacgaa gaaaccgaag tcgtgctctt cgagcccgcc gcgacgcgca
     1561 ataccggaag cgcggtcgag tcggagttca ccgccccgga cggcgcgcgg atttaaccgc
     1621 agaggacgca gagatcgcag agcaggaata tggtgggctt tcctctgcga tctctgcgtc
     1681 ctctgcggtt cttaacagcg tgcttgcggc ctcgcgcctg cgccctcaac ttgcagcgcg
     1741 tgcgtttacc aagaatcggg ttggcgttcc tcctcgcgag cgcgttcatt agccgcgcct
     1801 cggccgagga ttggaaaacc atccacgccg agggccgcgc ttacgtcagc ttcgccaacg
     1861 ttgcgcaatt ttaccgtttc ccgcgattca cccaggcaag ccgcagtgtc tcgttgcgcg
     1921 atgatcggcg cgccctcctc gcgcagggcg ggacgagcga gttctatatc aacggcgttc
     1981 gcttcttcag tgactatccg ctcctcgcca aaccggagga aaatctcatc tcggcgaccg
     2041 atgtggccaa gatcatcgag ccggtcctgc gaccgaataa aatcagcggc gcaaaaaaga
     2101 tcgatactgt ggtgctcgat cccggccacg gcggcggcga tagcggagcc tccggcccct
     2161 ggggcaacga aaaaagctac gccctcgacg tcgccctgac ggcgcgggaa aatcttctgc
     2221 gcgccggttt caaagtggaa atgacgcgtt cgcgcgacga aagcgtttcc ctcgaagatc
     2281 gggtcgcttt cgcgaatcga tttcccaacg ccgtgttcat cagcattcat ttcaactccg
     2341 ccgagggcgg ctccggggtg gaaacttatg cgcttgcccc ggagggcgta ccctcgaacg
     2401 cctcgaccga aagtcatccc tcgagcttcg acacccaggg gtatccgggg aacccgcagg
     2461 acccggccaa catcgccctc gccgccgccg tccacggcgc gatcctgtcg cgcgtttccg
     2521 ttttcgaccg cggcgtgcga cacgcgcgct ttcacgtcct gcgcgacatc aagatcccgg
     2581 ccgtcctggt ggaagcgggc ttcctgaacg atcgcatcga gggcgcgcac atcgccacgc
     2641 cgcaatatcg gcaacagctc ggactggcgc ttgcccaggc ggtcacgact tatgaccggg
     2701 cagtcaattt ccaatcctcc ggaccgacca tcgttgcggc gtcgaccaat cttccgccgc
     2761 acacgcactc tattaccgat tcgttaggcg atggagcgcc ggaccgagtc aacccgccgc
     2821 cggagccttc cgcggtcgtt catgcctcca aataaatgaa ccctcctgcg cgcgcaaccg
     2881 gggcgcctgc ccgggaaaaa ccgatcggcg ttttcgattc cggcatcggc ggactcaccg
     2941 tagtccgggc cctgcgcgaa ctcttgtcta acgagacaat cttttatctc ggagacaccg
     3001 cccgcgtccc ctacggcggg aaaagcgccg ccaccgtcga gcgctacagc ctcgagattg
     3061 cttcgctctt gttggaagag aattgcaaag cgatcgtcgt cgcctgcaac accgcctccg
     3121 ccctcgccct gccgaagctc gaagccacca cgcccgtgcc cgtaatcgga gtgatccaac
     3181 cgggcgcgaa ggccgccgtc gccgcgaccc gcaaccgcca catcggggtt atcggaacgc
     3241 gcgcgacgat caaaagcggg gcctacgaac gcgcaatcct ggccctcgat cccgcggtcc
     3301 gcgtctccgc ccgcgcctgt cccctttttg ttccgctgat cgaggaaggc tggttggagg
     3361 atgaaatcac cgatcgcgtc gttaggcaat atttggatcc actggtgcgg gacgggatcg
     3421 ataccctggt gctgggctgc acccattatc cgctgctccg cgaggcgatc ggacgctacc
     3481 tgggcgacgc ggtccggttg gtggactcgg cgcaaaattg cgcgaccgcc gtgcggcaat
     3541 tgctcgaagc cgaggacttg cgtgctaccg cggtgacggc cgggaaattg tccgtggcat
     3601 taaccgatcc gcccgacgcg ttcctcgacg tagcccaaaa agccctgcgc ctcgacatcg
     3661 gaacagtgca gctacgcaac gtgatccatc cggcgaagag ctgagaaagg ccgttggagg
     3721 gacgg
//
LOCUS       DATMNS010000138         4956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2337714, whole genome shotgun sequence.
ACCESSION   DATMNS010000138 DATMNS010000000
VERSION     DATMNS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4956
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2337714"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            401..802
                     /locus_tag="VNW28_04940"
     CDS             401..802
                     /locus_tag="VNW28_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXA09304.1"
                     /translation="MKKYWLVKQEPEAYSWNDFVRDGLTKWTGVRNFQARNNLRAMRK
                     GDAVLYYHSVSEKAAVGIAQVTREAFPDPTAKEGDWSAVELKPVKALDKPVTLEQIKA
                     EPKLRDIVLLRNSRLSVQPLGKADFELICRL"
     gene            1145..1927
                     /locus_tag="VNW28_04945"
     CDS             1145..1927
                     /locus_tag="VNW28_04945"
                     /inference="COORDINATES: protein motif:HMM:NF021601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXA09305.1"
                     /translation="MQRALVVSIHDVAPSTRTRVEKILAELRRHQVSACSLLVVPDYH
                     RQGRSLADPGFRSWLQELDASGHEIVIHGFYHQRARRENESGRDKVVTRFYTADEGEF
                     FDLDYAAALRLIREAREEFESHDFYPHGFIAPAWLLGAEAERAAIAAGMKYTATLRTV
                     RDFTDGQTFTSQSLVYSVRSAWRRTMSLAWNRSLFRSLTNNPLLRLGIHPPDIAQRAI
                     WRQIGKIIDLALRDRQSLTYRDWLRQESEIRPSRADQKSVMM"
     gene            1924..4329
                     /locus_tag="VNW28_04950"
     CDS             1924..4329
                     /locus_tag="VNW28_04950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA09306.1"
                     /translation="MSRCDLHLHSKFSDRSEDWIFRRFDFPDSYSNPPELYAQAKSAG
                     MDFVTLTDHDTIEGGLTLSHLSDTFLSEQVTTYFPQDPCKLNLLVWGISEGQHAQISS
                     LRENILDLQAFLQQETIAHAVAHPLYSINGKLSTSHLERLILLFQHFEGINGLRDALL
                     SDLTRHLLAQLTPAKVDEFANRHDLAPTHPDPWKKILVGGSDDHGGLFVGSVFTETPP
                     ATTPAQFLDHIRAGRCAPHGQGGTPLALSHGFYNTLASFIQDRFTAKLGPSAGLVEKM
                     FSRFMEGRDPTEFTLKEKAGFVTQGVLSGKIFELAKPGNLSLWRELSGYFAQPEVKAR
                     LAREVADVTEPERRTFIMANAACEQLAFRFFEKFVKQLNAGNVIESVQALASIAPILV
                     VLAPYIYAFHSQAPSRRWLRQICRDFTGAIPPQLQNRKRAWFTDTLEDVNGVSTTIRK
                     MAAEAREAGEELVVVTSRSELTLDGLPIKNFKPIGEFELPEYELQKLSFPPILQMLDY
                     IQRENFSEIIISTPGPIGLTALLAGKMLNLETSGIYHTDFPQYIRILTEDSFLESLAW
                     KYMQWFYGQLDTIFVNSEQYRRSWIARGIDPAKLKILPRGLDTRLFTPGRSEPDFWKQ
                     FGSNGDGVRLLFVGRVSREKDLDILVQAFRNLRQENIPVQLSIVGHGPFSKELAAMLP
                     EACYTGYLNGTDLARAYASSDIFVFPSTTDTFGNVIIEAQAAGLPVIVSDVGGPRELV
                     TDGVSGLITKARDPADFTSAIRRLVADESLRKEMSAAARRSVEDRSWPSAFRKFWAAT
                     EIS"
     gene            complement(4481..>4956)
                     /locus_tag="VNW28_04955"
     CDS             complement(4481..>4956)
                     /locus_tag="VNW28_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09307.1"
                     /translation="SPWISQDAATLRPGSGGWATVGVPRIIALAVLNGFGMHAFDDLP
                     VVRFAVDERDKDFLTPQYSFALEQNFRPERDYQANIRAVRQPLRVLAGQNDEVFYADK
                     FASVFKTAGKDVPVTLLPGIDHISLTLDQAALRAAISAVESMDHEKPDQTSEVVR"
BASE COUNT          992 a         1553 c         1444 g          967 t
ORIGIN      
        1 tttgccgagc gtgcggtaat gcagccgcag ctccgggagt gtggctccgg acgtgaattt
       61 gaagtcgcgc gcgataaagt cgccttcgtg aggcgcggga taatcggccg cctgcaaagc
      121 ggcgatcaga gccaccgacc agagcaacac cgcggttaga agtctcatgc gggaagagta
      181 ccgaaaaaca ccgccgcggc cacgagaata ggacgccgga ccggggagag atcgtcatcc
      241 tgagcgacgc gcagcggagt cgaaggatcc cgcggcgctg acttaaaggt tacgccacgg
      301 gctccctcga ctgcgctcgg gatgaccgcg agcgccgggt cgcgagcaag agacaaaacg
      361 cgtgatttcc cagacgcgac gattgtgtta tgggatcgcc atgaagaaat actggctcgt
      421 gaaacaggag ccggaagcct attcgtggaa cgactttgtg cgcgacggcc taacgaaatg
      481 gaccggggtg cggaatttcc aggcgcgcaa taatctccgc gcgatgcgca agggtgatgc
      541 tgtcctctat tatcatagcg tgagtgagaa ggccgcggtc ggcatcgcgc aggtgacgcg
      601 cgaagcgttt cccgacccga cggcgaagga gggggattgg agcgcggtcg agctaaagcc
      661 ggtgaaagca ctcgacaaac cggtgacgct ggagcagatc aaggccgagc cgaagctgcg
      721 cgacatcgtc ctgctgcgga attcgcgtct ttcggttcag ccgttaggca aagcggactt
      781 tgagctgatt tgccgcctgt aggcgcttcg ctgtgcgaag cgagggtctg ccgggaggct
      841 agcggtctcc gtctcgttgg ccggcaactc tcgcgctctc aggtgcgcgg ccttgtgggg
      901 agcgcacgcg actcgcgtgc tggttgctgc gacccgcagc aacgaacttt ctccctcggc
      961 tggtctgctc ccaagcactc tgaggggaaa ctcgagtccg cgatggcgag tcgccatcgc
     1021 cagcacgcgg gtcgcgtgcg ctccccaaaa aagctgacgg cgcgctgtgc ccaatttcaa
     1081 agttacgttg cactaggaag cagtttcggc ttgtccatgt tcctgcgatg gcgaggctac
     1141 gagcgtgcag cgcgcgctgg tggtttcaat tcacgatgtc gctccgtcga cccggacgag
     1201 ggtggagaaa atcctcgcgg aattgaggcg acatcaggtc agcgcctgct cgctcctcgt
     1261 cgtcccggat tatcaccggc aaggacgatc gctggccgat cccggtttcc ggtcatggct
     1321 ccaggaactg gacgcgagcg gacacgagat cgtcattcac ggattttatc atcagcgcgc
     1381 ccggcgggaa aatgaaagcg ggcgcgacaa agtcgtaacc cgtttttata cggcggatga
     1441 aggcgagttc ttcgatcttg attacgccgc tgcgctgcgc ttgatccgcg aggcccggga
     1501 ggagtttgaa agccatgatt tttatccgca cggtttcatc gcgcccgcct ggctgctggg
     1561 cgcggaggcg gagcgcgccg cgatcgctgc cggcatgaag tacacggcga ctctccgcac
     1621 agtgcgcgat ttcaccgacg ggcaaacctt cacttcgcaa tcgctcgtct acagcgtgcg
     1681 cagcgcctgg cggcgaacga tgagcctcgc ctggaatcgt tcgctgttcc gcagcctaac
     1741 gaacaatccg ttgctccggc tcggaattca tccgccggat attgcccagc gcgcaatctg
     1801 gcggcagatc ggaaaaatca tagatctggc attacgcgat cggcagtcgc tgacctatcg
     1861 tgactggctg cggcaggaat cagaaatccg tccgtctcgg gcggatcaga aatcagtaat
     1921 gatgtgagcc gctgcgacct tcatctgcat tcgaaattca gcgaccggtc ggaggactgg
     1981 atctttcggc ggttcgattt ccccgacagc tactccaatc cgccggagct ttacgcgcag
     2041 gccaaatcgg ccggaatgga ttttgtcacc ctgacggatc acgacacgat cgaaggcggt
     2101 ttaacgcttt cccatttatc cgatactttt ttgagtgagc aggttacgac ttattttccg
     2161 caggatccat gtaaacttaa tctgctcgtc tggggaattt cggagggcca gcacgcgcaa
     2221 atctccagcc tccgcgagaa cattctcgat ctgcaggcgt tcctccaaca ggagacgatc
     2281 gcccatgccg ttgcgcatcc gctctatagc atcaatggca agctcagcac ctcacacctc
     2341 gagcgcctga tcctgctctt ccagcatttc gagggcatca acggcctgcg cgacgcgctg
     2401 ctgagcgatc tcacgcgcca ccttctcgcg cagcttacgc cggcaaaggt cgacgagttc
     2461 gccaaccgcc acgacctcgc gcccactcat ccggatccct ggaagaaaat tctcgtcggc
     2521 ggctctgacg accacggcgg tctctttgtt ggctcggtct tcaccgaaac gccgcccgcc
     2581 acaacgccgg cgcaatttct cgaccacatt cgcgcgggtc ggtgcgcgcc ccacggacag
     2641 ggcggcacgc cgctcgcgct ctcgcacggt ttttacaaca cgctcgcgag ttttatccag
     2701 gatcgcttta ccgcgaagct cggcccgagc gccggcctgg tggagaaaat gttttcccgc
     2761 ttcatggaag ggcgcgaccc gacggagttc accctcaagg agaaagccgg cttcgtcacg
     2821 cagggcgttc tttccggcaa aatcttcgag ctggccaagc cgggcaacct gtccctctgg
     2881 cgcgagcttt ccggttactt cgcgcaaccg gaggtgaaag cgcggctggc gcgcgaagtc
     2941 gctgacgtca ccgagccgga gcgccggacc ttcatcatgg ccaacgccgc ctgcgaacag
     3001 ttggcgtttc gctttttcga aaaattcgtc aagcaactca atgccggcaa cgtcatcgaa
     3061 agcgtccagg ccctggccag cattgcgccc atcctggtgg tgctcgcgcc ttacatctat
     3121 gcctttcaca gtcaggcgcc ctcccgccgc tggctgcgcc agatctgccg tgattttacc
     3181 ggcgcgatcc cgccgcagct gcaaaaccgg aagcgcgcct ggttcaccga caccctcgag
     3241 gatgtcaatg gcgtctccac caccattcgc aagatggccg ccgaagcgcg cgaggctggc
     3301 gaggaactgg tcgtggttac ctcgcgcagc gaactcacgc tcgatggcct gccgattaaa
     3361 aacttcaagc cgatcggcga atttgagctg cccgagtacg agctgcaaaa gcttagcttc
     3421 ccgcccatcc tccagatgct cgattacatt cagcgcgaaa acttttccga gatcattatc
     3481 agcacgcccg gcccgatcgg gctgaccgcg ctccttgccg gcaaaatgct caatctcgag
     3541 acgagcggca tctatcacac cgatttcccg caatacatcc gcattctgac cgaggacagc
     3601 tttctcgaga gccttgcctg gaaatacatg cagtggttct acggccagct cgacaccatc
     3661 ttcgtcaact ccgagcaata ccggcggagc tggatcgcgc gcggcatcga ccccgccaaa
     3721 ctcaagattc tcccgcgcgg actcgacact cgtctcttca cccccgggcg gagcgagcca
     3781 gacttttgga aacaattcgg gagcaacggc gacggcgtgc gtcttttgtt cgttgggcgc
     3841 gtttccaggg agaaagacct cgacattctc gtgcaagcct ttcgcaatct gcggcaggaa
     3901 aatattcctg tgcagctctc gattgtcggc cacggaccat tctcgaaaga gctggccgcg
     3961 atgctgcccg aggcctgcta caccggctat ctcaacggca ccgatctcgc cagggcctac
     4021 gcttcgtccg acatcttcgt cttcccgagc acgaccgata ccttcggtaa cgtcatcatc
     4081 gaagcccagg ccgcgggcct gcccgtcatc gtctccgacg tgggcgggcc gcgggagctg
     4141 gttaccgatg gagtcagcgg cctcatcacc aaggcgcgcg atcccgccga cttcaccagc
     4201 gcgatccgcc gcctcgtcgc agatgagtcg ttacgcaagg agatgagcgc cgcggcccgg
     4261 cgtagcgtcg aggatcgcag ctggccgagt gccttccgga agttctgggc cgcgaccgag
     4321 atcagttagc cctggcctgc aaatggcccg aagttttcgg ggctggcgcg actacgtcgc
     4381 caatgacgaa accactatct cgatggcctc aaacacagcg gctcaccgct ggtttccaaa
     4441 ctgctgccta aactcgcgtt caataacttc actcaaatcc ttaacggacg acctcagatg
     4501 tctggtcagg cttttcgtga tccatgcttt cgacggctga tattgccgct cgcagagcag
     4561 cctgatcaag agtcagcgag atgtggtcga tccccgggag cagcgtgacg ggaacatcct
     4621 tacccgctgt cttgaagacg ctcgcaaact tatcggcgta aaacacctca tcattctgcc
     4681 cggccagaac ccgaagcggc tgcctgaccg cacgaatgtt tgcctggtag tcgcgctcgg
     4741 gcctgaaatt ctgctctagg gcgaatgaat attgcggcgt gaggaaatct ttgtccctct
     4801 cgtcgaccgc gaatctgacc accggcaggt cgtcaaacgc gtgcattcca aacccgttta
     4861 gcacggcgag cgcgatgatg cgcggaaccc ccacggtagc ccagccgccg ctgcccggac
     4921 gcaatgttgc tgcgtcctga ctgatccaag gagaaa
//
LOCUS       DATMNS010000139         2494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2701452, whole genome shotgun sequence.
ACCESSION   DATMNS010000139 DATMNS010000000
VERSION     DATMNS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2701452"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2057
                     /locus_tag="VNW28_04960"
     CDS             <1..2057
                     /locus_tag="VNW28_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXA09308.1"
                     /translation="VYYAIWAQTFDLKRVGTMPERNTVFDVDGKIYSRLAGANRLKVS
                     LRQVSPLFIQALLAREDTRFYEHGGIDWRGVVRALYRDILTGSAKEGASSLTQQLARN
                     SLPLGGRNLSRKLLEAMVALRLERELTKDQILELYVNRIYFGSGCYGVETASQTYFGK
                     NASKLDLSESALLAGLIRSPNRFSPLKNPHGAAVQRDTVLDRMQGLKKISATEAQRAK
                     RERIVSHPRRITVIQENYAMDAVQRDLNLLLTPDQIDNGGLFIYTTLDPVVQKSAADA
                     VEKQLAKIERQPGFNHPIKSQYRLPENGDDNAMPYLEGALVAIDNNSGGIRALIGGRD
                     YAQSKFNRALAPANRQIGSSFKPFVYTLAFIHGLLPGAAISDGPIQPGEIEGAGNWSP
                     ANSDGRYGGIKPASYGLIQSRNTMSVRVGEFAGLDETQKVATTLGLGDNIPHGPAIFI
                     GSFETDLKDLTAAYTVFPNAGVRKQAYIIERIDDPEHKPIYRAAHVTLPVLDPGALWM
                     TTELLEEVMTRGTAASASSLGFKLPAAGKTGTTNDFKDAWFIGFTSSITCGVWVGFDQ
                     PVTIVPHGYGSALALPVWTEVMLKAADRYPAEQFKAPVAMQEATVCSISNAIATSGCV
                     AAGTAYTIDLPVDRIPRGICKVHGGSPTLLGERVEQIQQKIKEAPRSILQSFKRFFGG
                     GN"
     gene            complement(2299..>2494)
                     /locus_tag="VNW28_04965"
     CDS             complement(2299..>2494)
                     /locus_tag="VNW28_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09309.1"
                     /translation="NASRALPLREGPDEQTIIDDNLAPKDGRESAVIMILEPNAAYTV
                     VVRGVGDTTGVALVEVYNLQ"
BASE COUNT          500 a          783 c          750 g          461 t
ORIGIN      
        1 tcgtctacta cgccatctgg gcgcagacgt tcgacctgaa acgggtcggc acgatgccgg
       61 agcgcaacac cgtcttcgac gtcgacggca aaatctacag ccggctggcc ggggccaacc
      121 gcctcaaggt ctcgttgcgg caggtctcgc cccttttcat ccaggcattg ctcgcgcgcg
      181 aagatacccg cttttatgag catggcggga tcgactggcg cggcgtggtg cgcgcgctct
      241 atcgcgacat cctcaccggg tcggcgaagg agggtgcgag cagcctcacg cagcaactgg
      301 cgcgcaacag ccttccgtta ggcgggcgca acctcagccg caagttgctc gaggcaatgg
      361 tcgcgctgcg cctcgagcgc gagctgacga aggaccagat cctcgagctt tatgtgaacc
      421 ggatttattt tggctccggt tgttacgggg tcgagaccgc gtcgcaaacc tatttcggaa
      481 aaaatgcgtc aaagctcgat ctctcggagt cggccttgct cgcgggtttg atccgcagtc
      541 ccaatcgttt ttcgccgctc aagaatccgc acggcgcggc tgtccagcgc gacaccgtgc
      601 ttgatcggat gcagggccta aaaaagatta gtgcgaccga ggcgcaacgg gcgaagcgcg
      661 aaagaatcgt ttcccatccg cggcgcatca cggtgatcca ggaaaactac gcgatggatg
      721 cggtgcagcg tgatctcaac ctgctcctca cccccgatca aatcgacaac ggcgggttgt
      781 tcatttacac gacgctggat ccggtcgtgc aaaagtccgc ggccgacgcg gtcgaaaagc
      841 agttggccaa aatcgagcgt cagccgggct tcaatcatcc catcaagtcg cagtatcgcc
      901 tcccggagaa cggcgacgac aacgcgatgc cttatctcga aggcgcgctc gtcgcgatcg
      961 acaacaacag tggcggcatc cgcgcgctca tcggcggacg cgattatgcg cagagtaaat
     1021 tcaatcgcgc gctcgcgccg gccaatcgcc agatcggctc ttccttcaaa ccgttcgttt
     1081 acacgctcgc cttcatccac gggctcctgc cgggcgcggc gatcagcgac gggccgattc
     1141 agccgggaga aatcgagggc gcgggcaatt ggtcgccggc gaattccgac ggccgctatg
     1201 gcggcatcaa gccggcttcc tacggcctta tccaatcgcg caacacgatg agtgtccggg
     1261 tcggcgaatt cgccgggctc gacgaaacgc agaaggtcgc taccacgctt ggcctgggcg
     1321 acaacattcc gcacgggccc gcgattttca tcggttcctt cgaaaccgac ctgaaggatt
     1381 tgaccgcggc ctacaccgtt tttcccaacg ccggcgtgcg caaacaggcc tacatcatcg
     1441 agcggatcga tgaccccgaa cacaagccga tctatcgcgc cgcccacgtc acgctgccag
     1501 tgctcgatcc gggcgcgctc tggatgacga ccgagctgct cgaggaagtg atgacccgcg
     1561 gcacggcggc gagcgcgagt tcgttaggct ttaagctccc cgccgccggc aagaccggca
     1621 caacgaacga tttcaaggac gcgtggttta tcggcttcac cagctcgatc acctgcggcg
     1681 tctgggtcgg gttcgatcaa ccggtcacga tcgttccgca cggctacggc tcggcgctgg
     1741 cgctgccggt ctggacggag gtcatgctga aagcggctga ccgttatccg gcagagcaat
     1801 tcaaggcgcc cgtcgccatg caggaagcga ccgtctgctc gatttccaac gcgattgcga
     1861 ccagcggctg cgtcgcggcc gggaccgcct acacgatcga tctgccggtc gacagaatcc
     1921 cgcgcggaat ctgcaaggtg cacggtggca gtccgacgct cctcggcgag cgggtcgaac
     1981 agattcagca aaagatcaaa gaggccccgc ggagcattct gcaatcgttc aagcgcttct
     2041 tcggcggcgg gaattgatcc gacgtggaag gggcctctgc gtcccgatcc gccctgggca
     2101 gggcgcggag ggacgtgctt acgcgcgtcc ggtcttgcca ggggacaaca aattgagcgt
     2161 gcgtcacaaa caagctggac aggcggaagc atgtccctcc gattcgctag gactaacgac
     2221 taactgcccg atccgacata ctcgttcgag taggtatcga tcgcgggtct aaccaatagc
     2281 acaatcggtc acatctcgct actgcaggtt gtaaacctcg actaaggcaa caccggtggt
     2341 gtcgcctaca ccacggacaa ccaccgtgta ggcggcgttc ggctccaaaa tcatgattac
     2401 ggctgattcc ctgccatctt tgggcgcgag attgtcgtcg atgatcgttt gttcgtcagg
     2461 gccttccctg aggggtaaag cccgacttgc gttc
//
LOCUS       DATMNS010000140        10152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_912406, whole genome shotgun sequence.
ACCESSION   DATMNS010000140 DATMNS010000000
VERSION     DATMNS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10152
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_912406"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VNW28_04970"
     CDS             complement(<1..279)
                     /locus_tag="VNW28_04970"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HXA09310.1"
                     /translation="MRVIAGRSGGIRLVVPKTDIRPTMDRVKAAIFSSLGDRVIGARV
                     LDLFAGTGALGIEALSRSAASVMFVESDRQAITAIERNLAQTGLSGRVR"
     gene            complement(276..1862)
                     /locus_tag="VNW28_04975"
     CDS             complement(276..1862)
                     /locus_tag="VNW28_04975"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXA09311.1"
                     /translation="MKKLRRLLLWLIGGLLVLAGMVLIGVNLYLQSQGTHHRIQQELS
                     QRLGTPLHLRQISVTPWSGLKLNGITIPQEPGKIGGDFLQAQTFQLRVQFWSLFSNRL
                     IIRQVSLIKPEVVWAQNSAGQWRLPELPPEKDVLVPPPRAAAGTTAASSLPASAAPAV
                     SATAPNEPALAAVPAPGFTPEVQRVNLKNGNFTFLDSAGRVIAKFTGLDFRSGFRTAT
                     EIAGNAKVEKISLRDRFFIEDLESPLRYAPAELGFSNISAHAAGGVINGRFEMELQEE
                     DSPFTAMVKFRDLQAEKLLINAGGPAGMLHGQVEGFLDASGKTADSNALAGHGEIILR
                     DGKLQQYSLLVALGQILQIDELMQLQLEQAEVKYHISPGIVTIDQLLLRSPNIRLSAT
                     GTINFKGRMRLNSQLALNDKVRRQLFTPIRENFQPINDPPGYAAVDFKISGTVDRPKT
                     DLMDKLVGRDLRDLGGVISSIFGHAKKKKTQNEAAPPSPTPGPSPNDAAPPVSPAPPT
                     PSLTPGESATETSATPAPSP"
     gene            1925..2788
                     /locus_tag="VNW28_04980"
     CDS             1925..2788
                     /locus_tag="VNW28_04980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXA09312.1"
                     /translation="MARTCVILNPTARSERARRWTERLRALCGDALLCATARAGEAEQ
                     LARNAVAEGFEQIVAAGGDGTIHEVVNGMADSDAALGLMPMGTMNVFANELGLPANDL
                     GRCWEIIRAEQTRLIDLPTANGKHFVQLAGVGLDAQVVKETSLAFKRNFGPLSYLISA
                     AQIAGRRPPRLLIESENALSEEGSFVLVGNGRLYGGPFPFFKHAVIDDGLFDVLVFKQ
                     LGYIELIKYLQNVIFTSDITTREVEYFQTRRLRVSSVEDVPVELDGELVGGCPVEFQM
                     KPRRLKVLAPG"
     gene            2974..3762
                     /locus_tag="VNW28_04985"
     CDS             2974..3762
                     /locus_tag="VNW28_04985"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXA09313.1"
                     /translation="MPRQRVVLLSLLGVNLALFIAQQMLQAYEPAVVPEYLGLSYRGI
                     DQAYAWQFLSALFLHAGVFQFAANMLVLYLLGRDVESILGQKHFLYLYFCGAVGGELS
                     HLFLMPATSVLLAASGGVAAVVVAFATILPEMELTESIFFLLPVKLKTKHLGYALCAL
                     GVLLLLFDRHGVVGHSSYLGGCAAGWLYAHLLGFGRPSWLQRALRERRVVREHRRQMS
                     LEEFIAEEIDPLLEKISRSGLESLTRGERRILAQAREKMPEPPQ"
     gene            3764..6496
                     /gene="glnE"
                     /locus_tag="VNW28_04990"
     CDS             3764..6496
                     /gene="glnE"
                     /locus_tag="VNW28_04990"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: protein motif:HMM:NF008292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HXA09314.1"
                     /translation="MAGADSLGEISRSIRPAQVAATLAALEANWPPDGPSLAETISGF
                     PLGTNALFHLLSVSSICGARLVQQPKILLWLAHPDTCADRRGYGRMLTELHNLADRAP
                     IAADNFRLLRLWKGREMVRIALREVAGAAPLEETMIELSQLADICLTTVYDHWNEELR
                     QRWGSPASQFAVIGVGKLGGRELNHSSDVDLIFVYSDEGQLTAKFSYHEWFTRLGNKI
                     IETFAASDPAGSLFRVDLRLRPEGRAGPLVRSLESMENYYAGFGEMWERLALIKARWI
                     CGDGELAYDFLRQLQPFIFPKSPTPDLLDEVAAIKRRIERDIVGHEQQERNVKLGAGG
                     IREIEFVVQALQLLHAARHPFLQEPSTLKAIRGLTELEFVPNEDAGQLDAAYCFLRRV
                     EHRLQIEAEQQTHNVPENGEALQCLARSLGFAAGANLLTTLREQMQRVRAIFQRVVGP
                     TARAKDSGALDFGIFRNPEQAAKTLAQLGESAAGFHVGPRTRQIFRKLRPLLLTWLAR
                     AADPDATLIQFVRFVEAYGFRSLLFELLAANPRLLELLVKTFDASEVAGSWLIRRPQL
                     LEELTRSGMLDRSFNVAQHLERLSSLGATSANLDPVRIYRQRELLRILLRDVLELAPL
                     LQLLAEQNDVAEACLVFVDRLLNPDEALTIVALGKFGGREIGYGADLDVLFVGDDPRA
                     AQQLVATLAQSSAEGRLAQLDARLRPDGEKGPLVCSLETFRAYYKTRAQFWEQQSLTR
                     CRAISGPLRFEFEALAKELWREAGGRSDLVRQIENMLVRIANERGSGNDFFDFKTGIG
                     GMIEGEFLVQALQMRHRVWEPNFLDAIGKLAAKKVLEKSDAERLRDAYSFLRRCESVL
                     RRWQNRGVSNLPTARDEEDRFARRMKFATIQDFHAPYGRAREVIRSLRLRYLAE"
     gene            complement(6515..7045)
                     /locus_tag="VNW28_04995"
     CDS             complement(6515..7045)
                     /locus_tag="VNW28_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HXA09315.1"
                     /translation="MTTHEDGPEIEFEILPPEEKRKRSELESLFRWLALIMDNLLRFP
                     GTNFRFGLDPLIGLLPGIGDTGSALISAFALLVAARRGLPKILLTRMSLNILINEAVG
                     IVPLFGDAFSFWFKSNARNYELLQRHAAAPRRSTKGDWLFVALVLGALFLILVVSLAV
                     SFWLLYEIARFLGLSR"
     gene            complement(7211..8608)
                     /gene="rho"
                     /locus_tag="VNW28_05000"
     CDS             complement(7211..8608)
                     /gene="rho"
                     /locus_tag="VNW28_05000"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXA09316.1"
                     /translation="MENQPPVQAPGVPFRRRRPSRRRSGRRNDSERGPAAFGNSSGDR
                     SERPSGTDTLQEDSREIERRREDAAEFAETPQGSGHTRAERPEGVSQNGEEPPREPEF
                     GEGIIEISGKGFGFLRDAKRNFVQTPQDIFVTPEIVRRFQLRDGMWIYGETRRGNRGP
                     QLTRLFKINDDEPTKYQDLRPFEDLTTINPNQRIKLETVSDRYTTRVMDLMTPLGKGQ
                     RGLIVAPPRTGKTTLLQHIADAVVKNHPEMKLIILLVDERPEEVTDFRRSCPNAEIMA
                     SSNDSDIKSHTRIAQLAVERAKRLVEAGKDVFILLDSITRTARAFNNATGGGGRTMSG
                     GIDARAMEIPRKLFAAARNTEEAGSLTIVATALIETGSRMDELIFQEFKGTGNMELVL
                     DRKISDQRTYPAVDIFLSGTRREELLLQPWELDKINLIRRGLAGHKPVEAIERLLMFV
                     KKFPTNAQMLKEIPG"
     gene            complement(8677..9432)
                     /locus_tag="VNW28_05005"
     CDS             complement(8677..9432)
                     /locus_tag="VNW28_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXA09317.1"
                     /translation="MAGHSKWSKVKRAKGALDVKRGTMFSKFSKEISIAARIGGGDPA
                     ANARLRSVIEMARAQSMPNDNIERAIKRGTGEGVDAQHFEEIVYEGYGPGGVAVIVET
                     ATDNKNRTAAEIRRIFSKNHGNLATSGSVSYMFHKKGQITVPRDTITEERLFELALEA
                     GAEELTTEEEQYVILTSHDQLYAVAEALKHASITIEGQKFTFIPDTTVPVTDEAAAQQ
                     VLRLCDSLEDDDDVQNVYSNLDIPEELLAKLPA"
     gene            9973..>10152
                     /locus_tag="VNW28_05010"
     CDS             9973..>10152
                     /locus_tag="VNW28_05010"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HXA09318.1"
                     /translation="MRKLRSGDERAFATFYDRFAPGLFSMIYAILHDQKESEDVLQEA
                     FLQMWKRTASYDASRS"
BASE COUNT         1822 a         2987 c         3259 g         2084 t
ORIGIN      
        1 ccgaacgcgg ccgctcaggc cggtttgcgc gagattgcgc tcgatcgcgg tgatggcctg
       61 gcgatcgctc tcgacgaaca tgacggaggc ggcgctgcgg ctaagcgctt caatgcccag
      121 cgccccggtg ccggcgaaga gatcaagaac gcgcgcaccg atgacgcggt cgccgagact
      181 cgagaaaatg gcggctttga cccgatccat cgttggccga atgtcggtct tggggacgac
      241 aagccgaatg ccgccggacc ttccggcgat gactctcatg gtgaaggcgc cggggtcgcc
      301 gacgtttcgg tcgcggattc gccgggcgtc agggatggcg tcggaggggc gggagagacg
      361 ggcggcgcag catcgttagg cgaaggaccg ggtgtcggcg agggcggagc ggcttcattc
      421 tgcgtttttt tctttttcgc gtgaccaaag atgctgctga tcacgccgcc gaggtcgcgc
      481 aggtcgcgcc cgacgagttt gtccatcaag tcggtcttgg gtcgatctac cgtcccgctg
      541 atcttgaaat cgactgcggc gtaaccgggt ggatcattaa tcggctgaaa gttttcacgg
      601 atcggggtga agagttgccg gcgaacttta tcgttcaacg ccagttgcga attgaggcgc
      661 atgcgaccct tgaaattgat cgtgccggtg gcggaaaggc ggatgttggg cgagcgcagg
      721 aggagttgat cgatcgtaac gatcccgggg ctgatgtgat atttcacttc cgcttgttcc
      781 agttgcagtt gcatcaactc atcgatctgg agaatctggc cgagcgcgac gaggaggctg
      841 tattgctgca attttccgtc gcgtaaaatg atctcgccgt ggcccgcgag tgcattgcta
      901 tcggccgtct tgcccgaagc atcgaggaac ccttccacct gcccgtgaag catgccggcc
      961 ggaccgcccg cgttgatcag taatttctcg gcctggagat cgcggaattt caccatggcg
     1021 gtgaaaggag aatcttcctc ttgcagctcc atctcgaagc ggccgttgat cacgcctccg
     1081 gcggcgtgcg cagaaatatt ggaaaaaccg agttccgccg gagcgtagcg caggggcgac
     1141 tcgagatcct cgatgaagaa cctgtcgcgc agggaaattt tttcgacttt cgcgttgccc
     1201 gcgatttccg tcgcggtgcg gaagccggag cggaaatcga gcccggtgaa tttggcgatc
     1261 acccgaccgg cggaatcgag gaaggtaaag ttgccattct tcaaattgac gcgctgcact
     1321 tccggggtga aaccgggcgc gggcactgct gccagggccg gctcgttagg cgcggtcgcg
     1381 ctcacggctg gtgcggcgct ggccggaaga gaggaggccg cggtggtgcc ggccgccgcg
     1441 cgcggcggtg ggacgagcac gtccttctcc ggcggtaact cgggcagacg ccattgtccc
     1501 gcggaattct gcgcccagac gacttcgggc ttgatcagcg atacctgccg gataatgagc
     1561 cggttgctga agagcgacca gaattgcacc cgcagctgaa aggtttgcgc ctggagaaag
     1621 tctcccccga tcttgcccgg ttcctgcgga atggtgatcc cgttaagttt taatccgctc
     1681 cagggggtga cgctgatctg gcgcaaatgc agcggggtcc ccaaccgctg gctgagttcc
     1741 tgctgaatgc gatggtgtgt gccctgcgat tgcagataga gattcactcc gatcaacacc
     1801 atgccggcca aaaccaatag tcccccgatg agccagagca ggagccggcg aagttttttc
     1861 accgtgggaa gctacgcagc gcgggattga agaaaagatg aaacccgcct aacaaggtaa
     1921 tctcttggcc aggacctgtg tcattctcaa cccgaccgcg cggagcgaac gtgcccggcg
     1981 ctggacggag cggttgcgcg cactgtgcgg cgacgccttg ctctgcgcga cggcgcgggc
     2041 tggggaggcg gagcagctgg cgcgcaacgc ggtcgccgaa ggcttcgaac agatcgtcgc
     2101 ggcgggcggt gacggaacga tccatgaagt tgtcaatggg atggccgatt ccgacgcggc
     2161 gcttggcttg atgccgatgg gcacgatgaa cgtcttcgcc aacgagctcg gcctccccgc
     2221 gaacgatctt gggcgctgct gggaaattat tcgtgcggaa caaacgcggc tgatcgattt
     2281 gcctacggct aacggaaagc attttgtcca gctggccggg gtcgggctcg atgcgcaggt
     2341 ggtgaaggaa accagccttg ccttcaaaag aaacttcggc ccgctcagtt atctcatctc
     2401 ggccgcgcag atcgctggcc ggcgtccgcc gcggttgttg atcgagtcgg aaaacgcgct
     2461 ctcggaggag ggatcgtttg tcctcgtcgg caacggccgg ctttacggtg gcccgtttcc
     2521 tttcttcaag catgcggtga tcgacgacgg gctgttcgat gtgctcgtct tcaagcagct
     2581 gggctacatc gaattgatca agtatttgca gaacgtcatt ttcacctccg acatcacgac
     2641 gcgggaggtg gaatatttcc aaacccggcg cttgcgggtg agcagcgtgg aagacgtgcc
     2701 cgtcgagctg gatggggaat tggtcggggg ttgcccggtg gagtttcaaa tgaagccgcg
     2761 gcgcttgaag gtgctcgcgc ctgggtagac gctccgctcc ccgaagcgcg cgggacgacg
     2821 gtgatcggca ggcaatgccg atagcctcgc tacgcaaaag gaggtgtcga ccgagcgccg
     2881 gcctttgatt cccaatgaca acccgaagcg tttgcgatat tttcaggtat gcgtttgtcc
     2941 ggccgaaaag cttcgtcgca cgcaccccgc tggatgcccc ggcagcgcgt cgttttgttg
     3001 tccttgctgg gtgtcaatct cgctcttttc atcgcgcaac agatgctcca ggcgtatgaa
     3061 ccggccgttg tccctgagta tctcggcctg agctatcgcg gcatcgacca ggcttacgcc
     3121 tggcagtttt tgagcgcgct gtttttgcac gccggcgttt tccaattcgc cgcgaacatg
     3181 ctcgtgcttt atctcctcgg tcgcgacgtg gaatcaatcc tcggacaaaa acatttcctt
     3241 tatctctact tctgcggcgc ggtcggcggt gagctcagcc accttttcct catgcccgcg
     3301 accagtgtgt tgctcgcggc ttccggcggc gtcgcggcgg tggtcgtggc tttcgccacc
     3361 attctgccgg aaatggaact gaccgaatcg atctttttcc tcctgccggt aaagctcaag
     3421 acgaagcacc tcggttatgc cctctgcgcg ctcggcgttc tgctcctgct gttcgaccgc
     3481 cacggcgtgg tcggccatag ctcttatctg ggcgggtgtg ccgcgggttg gctctatgcg
     3541 catctcctcg ggttcggccg accgtcgtgg ttgcagcggg cgctgcgcga gcggcgggtg
     3601 gtgcgggagc accgccgcca gatgtcgctc gaagaattca ttgcggagga gatcgatcca
     3661 ctcctggaga agatttcgcg gagcggcctc gagagcctga cccgaggcga gcgacgcatt
     3721 ctggcacagg cgcgcgaaaa gatgcccgaa ccaccgcaat agcgtggcgg gtgcggactc
     3781 gttaggcgag atttcccgga gcattcgccc ggcgcaggtg gcggcgacgc tggccgcgct
     3841 ggaggccaac tggcccccgg atggtccgtc gctggccgaa accatctccg gctttccgtt
     3901 aggcacgaac gcgctttttc atctcctttc ggtttccagc atctgcggcg cgcgcctggt
     3961 tcaacaaccc aaaatcctcc tctggctcgc gcaccccgat acctgcgccg atcgccgcgg
     4021 ctacgggcgg atgctgaccg agctgcacaa tctggccgat cgcgcgccaa tcgcggccga
     4081 taactttcgt cttctccgtc tctggaaagg gcgcgaaatg gtccggatcg cgttgcgcga
     4141 ggtggccgga gcagcgcccc tcgaggaaac aatgatcgag ctgtcgcagc tggccgatat
     4201 ttgcctaacg accgtttacg accattggaa cgaagaatta cgccagcgct ggggttcgcc
     4261 cgcaagccag ttcgcggtca tcggggtggg caaactgggc gggcgcgagt tgaaccacag
     4321 ttccgacgtc gacctgattt tcgtctacag cgacgaggga cagttgaccg cgaagttcag
     4381 ctatcacgaa tggttcacgc ggcttggcaa caagatcatc gaaaccttcg cggcctccga
     4441 cccggcgggc tcgctctttc gcgtcgacct gcgccttcga ccggaaggac gcgcggggcc
     4501 gctcgttcgg tcactcgaga gcatggagaa ttactacgcg ggcttcgggg agatgtggga
     4561 acgcctggcg ttgatcaaag cgcgctggat ctgcggcgac ggcgagctgg cctacgactt
     4621 cctgcgccag ttgcagccgt tcatttttcc gaagagcccg acgcccgacc tgctcgatga
     4681 ggtcgccgca atcaagcgcc ggattgagcg cgacatcgtc ggccacgagc agcaggagcg
     4741 aaatgtgaaa ctcggcgccg gtggcattcg cgagattgag ttcgtcgtgc aggcgctcca
     4801 gcttttgcat gctgcgcggc acccgttttt gcaggagccc agcaccctga aagcgatccg
     4861 cgggctgacc gagctggagt ttgtgcctaa cgaggatgcg ggacaactcg atgcggccta
     4921 ttgcttcctg cgccgggtcg agcatcggct ccagatcgag gccgagcagc aaacacacaa
     4981 cgtgccggag aatggcgaag cgctgcaatg cctcgcgcgc agcctcggct ttgccgctgg
     5041 cgccaatctg ctgaccacct tgcgcgagca gatgcagcgg gtgcgcgcga tcttccagcg
     5101 ggtggtgggt ccaacggcca gggcgaagga ttccggcgcg ctcgatttcg gcattttccg
     5161 caacccggag caggcggcaa agaccctcgc ccaactcggc gagagcgcgg ccggctttca
     5221 tgtcgggccg cgcacccggc agattttccg gaagctgcgg ccgctgctcc tcacctggct
     5281 ggcgcgcgcg gccgatccgg acgcgacctt gattcaattc gtgcgcttcg tcgaggctta
     5341 cggcttccgc agccttttat ttgagttgct cgcggcgaat cctcgtctcc tcgagctgct
     5401 ggtcaaaacc tttgacgcga gcgaggtcgc cggcagctgg ctgatccgtc gcccgcaatt
     5461 gctcgaggaa ctgacgcgca gcgggatgct cgatcgctcg ttcaatgtgg cgcaacatct
     5521 cgagcgttta agttcgttag gggcaacgag cgccaatctc gatccggtgc ggatttatcg
     5581 gcagcgcgag ctgttgcgca tcctgttgcg cgacgtgttg gaactggcgc ctctgctgca
     5641 gttgctcgcg gaacagaatg atgtcgcgga agcctgtttg gtttttgtcg accgtctctt
     5701 gaatccggac gaggccctca ccatcgttgc gctcggaaaa ttcggcggcc gcgaaattgg
     5761 ctacggcgcc gatctcgacg tcctgttcgt gggtgacgat ccgcgcgcgg cgcagcagct
     5821 ggtcgcgacg ttggcgcaat ccagcgcgga aggccgcctc gcgcagctcg acgcccgcct
     5881 ccgtccggat ggcgaaaaag ggccgctggt ttgttcgctc gaaaccttcc gggcgtacta
     5941 caaaacgcgc gcgcagtttt gggagcagca gtcgctgacc cgctgtcggg cgatcagcgg
     6001 gccgctccgt tttgaatttg aggccctcgc caaggagctc tggcgggagg cgggcggtcg
     6061 ttccgatctc gttaggcaaa tcgaaaacat gctcgtccgg atcgcaaacg agcggggcag
     6121 cggaaacgat tttttcgact tcaagaccgg gattggcggc atgatcgaag gcgaattttt
     6181 ggttcaggcg ctgcaaatgc gccaccgcgt ctgggagcca aactttttgg atgcgatcgg
     6241 aaaactggcg gcgaagaagg ttctggagaa gagcgacgcg gaaagactcc gcgatgccta
     6301 tagttttttg cgccgctgcg agtcggtcct ccgccgctgg cagaaccgcg gggtctcgaa
     6361 cctgccgacc gcgcgcgacg aagaagaccg cttcgcgcgg cggatgaaat tcgcgacgat
     6421 ccaggatttt cacgcgcctt acggccgggc gcgagaggtg attcgttccc ttcggctgcg
     6481 ctacctggct gagtaggatt tgcgctcgcc tgattcatcg gctcaatccc aggaacctcg
     6541 cgatttcgta gaggagccag aaactgacgg ccagactaac gacgaggatc aggaacaagg
     6601 cgcccaaaac gagcgcgacg aaaagccagt cgcctttcgt cgagcgacgt ggcgcggcgg
     6661 cgtgccgctg tagcagctcg tagttgcgcg cgttggactt gaaccagaag gaaaaggcat
     6721 cgccgaagag cgggacgatg ccgacggctt cgttgatcag gatgttaagc gacatgcgcg
     6781 tgagcaggat ctttggtagt ccgcgccgcg ccgcaacgag gagggcgaac gcggagataa
     6841 gggcggaacc ggtgtcgccg atgcccggca gtagtccgat gagcggatcg agtccgaagc
     6901 ggaaattggt tccgggaaag cggaggagat tgtccatgat gagcgcgagc cagcggaaga
     6961 gtgattcgag ttcggatcgc ttccttttct cctccggcgg gaggatctca aactcaatct
     7021 ccggcccgtc ttcgtgggtc gtcatcctga gcaaagcgta gtggagtcga aggatcccgt
     7081 ggcggaacgg gcgtcctcct tcagcccggg gcggggcagg aaatattgcc agtccccgac
     7141 gagatcaagg cttcctggtg acacgacggg atccctcgac tgcgctcggg atgaccaggg
     7201 tgggacgccc ttaacccgga atttctttca gcatctgcgc attggtcggg aatttcttca
     7261 caaacatgag cagacgctca atcgcttcga ccggcttgtg cccggcgaga ccgcgacgga
     7321 tgagattgat tttgtcgagc tcccagggct ggagcaacaa ttcctcgcgg cgcgtgccgg
     7381 acaggaaaat atcgaccgcc ggataggtgc gctggtcgct aatcttgcgg tcgagcacga
     7441 gctccatgtt gccggtgcct ttgaactcct ggaagatcaa ctcgtccatc cggctgccgg
     7501 tttcgatcaa cgccgtggcc acgatcgtga gggacccggc ttcctcggtg ttgcgcgcgg
     7561 ccgcgaatag ttttcgcgga atctccatcg cgcgggcgtc gatgccgccg ctcatcgtgc
     7621 gtccgccacc gccggtcgcg ttattgaaag cgcgcgccgt gcgggtgatc gagtcgagca
     7681 gaatgaagac atccttgccc gcttcgacca ggcgcttggc ccgttcgacg gcgagctgtg
     7741 cgatgcgggt atgactcttg atgtcgctat cattggagct ggccatgatc tccgcgttgg
     7801 gacaggagcg ccgaaagtcg gtcacttcct cggggcgttc atcaaccagg aggatgatca
     7861 atttcatctc cggatgattt tttacgaccg catcggcaat gtgctggagc agcgtggtct
     7921 tgccggtgcg cggtggagcg acgatcaagc cgcgctgacc tttgccgagc ggcgtcatca
     7981 aatccatcac ccgcgtggtg taacgatcgg agacagtttc gagtttgatc cgctgattcg
     8041 gattgatcgt ggtcaggtct tcaaagggtc gcaggtcctg atacttggtc ggttcgtcgt
     8101 cgttgatttt gaacagacga gtcagttgtg ggccgcgatt gccccggcgg gtttcgccgt
     8161 aaatccacat gccgtcgcgg agttgaaagc gccgcacgat ctccggcgtg acgaaaatat
     8221 cctgcggcgt ctggacgaag ttgcgtttcg catcacgcag aaaaccgaaa cctttcccgg
     8281 aaatctcaat gataccttcg ccgaactccg gttcgcgtgg tggttcttcg ccattctggg
     8341 agacgccttc cgggcgttca gcgcgggtgt gcccggaccc ttgcggcgtc tcggcaaatt
     8401 ccgccgcatc ctcgcggcgt cgttcgatct cgcgggaatc ttcctggaga gtgtccgttc
     8461 cactcgggcg ctccgaccga tcgccactgg agttgccgaa agcagcgggt ccgcgctccg
     8521 aatcattgcg gcgcccggaa cggcggcggg acgggcgtcg tctccggaac gggacgccgg
     8581 gtgcctggac aggcggttgg ttttccattt cttcgctcat aattgaaacc ggtttggctc
     8641 gaggcgacac gaggaccggt gccggctcgc agttctctac gctggcaact tggccagcaa
     8701 ctcttcaggg atatctaagt tggaatagac gttttgaacg tcatcgtcgt cttcgaggct
     8761 atcgcagagg cgcaggacct gttgcgcagc cgcctcgtcg gtgacgggga cagtcgtgtc
     8821 gggaatgaaa gtaaactttt gcccctcaat cgtgatgctc gcgtgcttga gcgcctcagc
     8881 tacggcatag agctgatcgt gggaagtgag aataacatac tgttcctcct cggttgtcaa
     8941 ctcttctgcc ccggcttcga gagccaattc aaagaggcgc tcctccgtaa tcgtatctcg
     9001 tggcaccgtg atctgtcctt ttttgtgaaa catgtaagaa acgctcccgc ttgttgccag
     9061 gttcccgtgg ttcttgctaa agatgcgtcg gatttcggcc gccgtgcggt tcttgttgtc
     9121 ggtcgccgtc tccacgatca ccgccacgcc gcccggtccg taaccttcgt agacgatttc
     9181 ttcaaaatgc tgggcgtcga ccccctcgcc ggtcccgcgt ttgatcgcac gctcgatgtt
     9241 gtcgttaggc atgctttgcg cccgggccat ctcgatcacg ctccggaggc gcgcatttgc
     9301 cgccggatcg ccgccgccaa tccgcgccgc gatgctgatc tctttcgaga acttgctgaa
     9361 catcgtgccg cgtttgacgt cgagcgcgcc tttggcgcgc ttcaccttgg accatttgct
     9421 atgtcccgcc atggaatggg gatgggatga gttacaaaaa cctgttgccg gacggacctg
     9481 cgttgcgcgg gggagcggaa ccggggcgcc ctcgaaagag aggactgacg aggaggcaaa
     9541 ccgacaccca ttgaatcgta ttgcgcgccg cttgtcaacc ttcgcggtgg gttcgcagcc
     9601 gcccgcggga gtgcagaagc ctcgctctgc gaggtgcgcg cgcaagcgcg gcggccgcgt
     9661 ccggtccggc gcttccagag cgaagcgtct ccaggcgcgt gcctgcggca gcgacgcgat
     9721 tgcatctggc gagcggtttc gcacgtctta accaaatgtg agaacgagtt tccctgcgaa
     9781 ccacggggct gccggcgcga tcgctgattt gggaccgcgc ccaagcgcgg cgcgccgatc
     9841 gtcccttgct cacgcgcgat ttctccttca ggttcggggc gcgtggcgct cgaaccttcc
     9901 agtattccgc aggagtcaag cgcgcccgtc cgccgggcca tccaaccggc caatgaagca
     9961 gatgttgagc tcgtgcgaaa attgcggagc ggcgacgaac gggcttttgc cactttctac
    10021 gatcgttttg cccccggtct tttttccatg atttacgcga tcctgcacga ccagaaagaa
    10081 tccgaggacg tgctccagga ggccttcctc caaatgtgga agcgaacggc gtcctacgat
    10141 gcgagccgga gc
//
LOCUS       DATMNS010000141         2899 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_198741, whole genome shotgun sequence.
ACCESSION   DATMNS010000141 DATMNS010000000
VERSION     DATMNS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198741"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1089)
                     /locus_tag="VNW28_05015"
     CDS             complement(<1..1089)
                     /locus_tag="VNW28_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09319.1"
                     /translation="MKRKRIVVMGFMGSIPIAGVIWQHIHYIVGLQRLGHDVYYIEDS
                     ARIPYNPQTFDTSNDYSYAAQVLAQLANEFGFENRWAFCARYLPGNPTAGLRLTKIQQ
                     LYLEADAILNVCGTQEFNPDLLKSDSILYVESDPGVEQIKVDKRIRSTIDYLKRHRAL
                     FTFGENVGGKRFPVPLHKLKWLPTRQPVVTDLWKTRRVPAKAAVFTSIANWSTSGLKD
                     ITWRGEKYLWSKSREFLRFVAAPKKSGELFELATDIKDAPTREKFLRNGWRFRGPHEM
                     SVDYWQYRDYIRRSKGEFTVAKDQYVRLHTGWFSDRSACYLAAGRPVITQETGFTDHY
                     GNHGGLFAFRSLGEIAEAVRAINADYRKQ"
     gene            complement(1247..1840)
                     /locus_tag="VNW28_05020"
     CDS             complement(1247..1840)
                     /locus_tag="VNW28_05020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HXA09320.1"
                     /translation="MSNVNRDRKIEGDWWAEPIPPNVQFGAGLYCETAQIFRFLRNRR
                     ADALRLGDYVSCYAGCSFSIGVNGRCTVGDFTLLNGAIVMAEELIEIGSYCLISWNVG
                     IADSDFHPIDPAQRRIDALALAPYYKERPPRPPIRTAPVRIADNVWIGMGAVILKGVT
                     IGENSVVAAGSIVSKSVAPNTVVAGNPAVVVKELGAP"
     gene            complement(1928..>2899)
                     /locus_tag="VNW28_05025"
     CDS             complement(1928..>2899)
                     /locus_tag="VNW28_05025"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA09321.1"
                     /translation="GGTAAMYYLGTLHVLAGTLTLGSLILFIAYLTMLYQPLESLTYT
                     AWAMEGATAGAKRCFEVLDSEDDVRDSSGAVEISSTHAAIAFQQVSFSYDEQHPVLRG
                     IDLHISPNQIVALVGGTGAGKSTLLSLVPRFYDPSAGVVSLDGRDVREITKKSLRAQV
                     AIVLQDTLLFSTTIRENIAYGRPGATDAEIREAARRAQADDFIRHLPNGYDSAVGERG
                     GHLSVGQRQRIGIARAFLKDAPILLLDEPTSALDPTTEAAIMETIKELMHGRTTLIVT
                     HRLATIHNVDQIVVLEHGQIVESGRGAELVARGGAYAKLYASGNYPG"
BASE COUNT          571 a          818 c          940 g          570 t
ORIGIN      
        1 ctgctttcta taatcggcgt tgatcgcgcg gaccgcctcg gcgatttcgc ctaacgagcg
       61 gaaggcgaac aatccgccgt ggttgccata gtgatcggtg aagccggtct cctgcgtgat
      121 gaccggccgc cccgctgcca ggtagcaggc gctgcggtcg ctgaaccagc cggtatgcaa
      181 ccgcacgtat tggtctttcg cgaccgtgaa ctcgcctttc gagcgccgga tgtaatcgcg
      241 atattgccag taatcgacgc tcatctcgtg cgggccgcgg aaacgccagc cgttccgcag
      301 aaatttctcc cgcgtcggcg cgtctttgat atcggtcgcc agttcgaaca attcgccgga
      361 ttttttcggc gcggcgacga agcgcagaaa ttcgcgcgac ttgctccaga gatatttttc
      421 accgcgccag gtgatgtctt tcaggccgct ggtcgaccag ttggcaatgg aagtgaagac
      481 cgccgccttc gctggcacgc gtctggtttt ccagagatcg gtcacgacgg gctgccgggt
      541 cgggagccac ttcaatttgt gcaacgggac cggaaaacgt ttgccaccga cgttttcgcc
      601 gaaggtgaaa agggcgcgat gtcgtttgag ataatcgatc gtcgagcgga tgcgcttgtc
      661 gaccttgatc tgctcgacgc ccggatcgct ctcgacatag agaatgctgt cgctcttgag
      721 cagatccgga ttgaattcct gggtgccgca gacgttcagg atcgcgtcgg cctcgaggta
      781 aagctgctgg attttcgtga ggcgtaatcc agcggtcgga ttaccgggca ggtagcgggc
      841 gcaaaaagcc cagcgatttt cgaacccgaa ttcgttggcc aattgcgcca ggacctgcgc
      901 ggcatacgag tagtcgttac tcgtgtcaaa ggtctgcggg ttgtagggaa tccgggcgga
      961 atcttcgatg tagtaaacgt cgtgcccgag ccgttgcagg ccgacgatat aatggatgtg
     1021 ctgccagatg acaccggcga tcgggatcga gcccatgaag cccatcacga caattcgctt
     1081 ccgtttcatt ggggcgaaga ctccgggaga gggggagggg aaaacatcca acgctccacg
     1141 ttcaacgtcc aacgtccaac gaaaggcagg cgactcgcag tgcctacctc tggggttgaa
     1201 cgctgaacgt tgaatgttgg acgttgaacg tttctgaaga acaaactcat ggtgcgccga
     1261 gctctttcac caccaccgcg ggattccccg cgaccacggt gttgggcgcg acgctcttcg
     1321 agacgatcga gccggcggcg acgacggagt tttcgccaat cgtcacgcct ttcaaaatga
     1381 cggcgcccat tccgatccag acgttatcgg cgatgcggac gggcgcggtg cggatgggcg
     1441 ggcgcggcgg gcgctccttg taatagggcg cgagcgcgag cgcgtcgatg cggcgctgcg
     1501 cgggatcgat cggatgaaaa tcggaatcgg cgatcccgac attccacgag atgaggcaat
     1561 agctgccgat ctcgatcaat tcctcggcca taacaatggc gccgttcaag agagtaaaat
     1621 cgccgacggt gcagcggccg ttgacgccga tggaaaacga gcaaccggcg taacaggaaa
     1681 cgtagtcgcc caggcgcagg gcgtcggcgc ggcggttgcg gaggaaacgg aagatttgcg
     1741 cggtctcgca ataaaggccg gcgccgaatt gaacgttagg cgggatcggc tcggcccacc
     1801 agtcgccctc gatcttacgg tcgcggttga cgttgctcat gcgccggagt ttctaaccac
     1861 gaagaacacg aagtgcacga aggaatttgc agttctgagg atgtttccac tgcgtgacct
     1921 tcgtggttca accggggtag ttgccggagg cgtagagttt cgcataggcg ccgccgcgcg
     1981 ccaccagctc ggcgccgcgc ccgctctcga cgatttggcc gtgctcgagg acgacgatct
     2041 gatcgacgtt gtgaatggtg gcaagtcgat gcgtgacgat gagagtggtg cggccgtgca
     2101 tcagttcctt gatcgtctcc atgatggcag cctcggtcgt tgggtcgagc gcggaagtgg
     2161 gctcgtcgag gagcaggatc ggcgcgtctt tgagaaaagc gcgcgcgatg ccgatgcgct
     2221 gccgttggcc gacactcaag tgtccgccac gttctccgac cgcgctgtcg tatccgttag
     2281 gcaaatgccg gatgaaatca tccgcctggg cccggcgcgc ggcttcgcga atctcggcgt
     2341 cggtcgcgcc gggccggccg taggcgatat tttcgcggat ggtggtggaa aaaagcagcg
     2401 tgtcctggag gacaatcgcg acctgcgcgc ggagcgattt cttggtgatc tcgcgcacgt
     2461 cgcgaccatc aagcgaaacg acgcccgcgc tcggatcata aaagcgcggg accagactaa
     2521 gcagggtgct cttgccggcg cccgttccgc cgaccagtgc gacgatttgg ttgggcgaaa
     2581 tgtggaggtc gattccgcgc agcaccgggt gttgctcgtc gtagctgaaa ctgacttgct
     2641 ggaaggcgat cgcggcgtgc gtgctggaaa tctcgaccgc gccggaggaa tcacggacat
     2701 cgtcttcact atcgagcacc tcaaagcaac gcttcgcgcc cgcggtcgcg ccttccatcg
     2761 cccacgcggt gtacgtaagc gactcgagcg gctggtacaa catcgtgaga taagctatga
     2821 agaggatcag cgagccaagg gtgagcgtgc cggcgagaac gtgcagtgtg ccaaggtaat
     2881 acattgcggc ggtgccccc
//
LOCUS       DATMNS010000142         4208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1628825, whole genome shotgun sequence.
ACCESSION   DATMNS010000142 DATMNS010000000
VERSION     DATMNS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1628825"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VNW28_05030"
     CDS             complement(<1..1095)
                     /locus_tag="VNW28_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HXA09322.1"
                     /translation="MEKTARSLTERLRAAGHVAYFAGGCVRDLLRGMTPQDIDIATNA
                     RPAEVQKIFGRTYAVGAHFGVIVVLENDFQFEVATFRSDGAYLDGRRPAEVHFSSAEE
                     DARRRDFTINGLFFDPPNEEVIDFVGGREDLAAKVIRAIGDPAQRFAEDRLRLLRAIR
                     FATVLEFEIEPATWEAVGRAAATIGEISAERIRDELVRIFLSPQRVRGWDLLDASGLM
                     EQVLPELVAMKGCAQPPQFHPEGDVFQHTRLMLGLLPNDVSLPLVLSVLFHDIGKPAT
                     SSVDDAGRIRFNGHDRVGAEMTEALMHRLRFSRAEIDATVEAVRQHMVFKDVPNMRVA
                     KLKRFMARPTFADELELHRVDCAGSHGMLDN"
     gene            complement(1607..2086)
                     /gene="lspA"
                     /locus_tag="VNW28_05035"
     CDS             complement(1607..2086)
                     /gene="lspA"
                     /locus_tag="VNW28_05035"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXA09323.1"
                     /translation="MKIFFYLSLPLYALDQVTKYFVFHFVDPSEPRDLIPNFFTLVHV
                     TNTGAAFGSFRDNNRFFVAISCIALAVVTVLMLRREPRDPWRRIALGLLMAGILGNLT
                     DRLLHGYVIDFLLFDLHIPFAHPWPAFNVADSCICLAVACFMIYSFRDSKAKAQAGH"
     gene            complement(2131..>4208)
                     /locus_tag="VNW28_05040"
     CDS             complement(2131..>4208)
                     /locus_tag="VNW28_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXA09324.1"
                     /translation="RYVVQKFNVGGAFVPRSAGATPADPAPIGAQRPLPHSETLVLAG
                     KMVAPFSAATGFEPVGEPLENFPGNKLEGIGAQHPFLPRTAIVLTADFVTMDTGTGSV
                     HIAPGHGEDDYSLGKANGLPILSPVDDLGRYTAEVGLPELVGQYVFDANADIVRLLRA
                     RGMLVAEQPFRHSYPYCWRSKTPIIFRAVEQFFIRIDDLRKNALRAITTVTWLPAWGE
                     NRIAGTVESRPDWVISRQRSWGVPLPVFYSAAGESLLNPEWIRKLADLVAERGSNIWF
                     ELDDVALAAALGLPAGTRHRNDTIDVWIDSGVSHQAVCATNPALRDPADMYLEATDQH
                     RGWFQSSLMTSVALHDRAPYKICVTHGFVVDVDGKKISKSGTYNKPMDAGHFVGRHGA
                     DLVRLWVSSINYTDDVPFSEEMFTRLGDTYRRIRNTLRILLGNLADFESGKQESRKEE
                     FTFVDRWILDRLQQVMEDCRAAYAAYEFHKVYHTLNQFCAVDLSSLYVDITKDRLYCD
                     APAAPRRRATQTAMSHIFDALCQLLAPILVFTAEEAWSHRHPSTPLRSAPDDASVHLQ
                     LFPEPQAAWRDAALRTEGEELLRLRGVIGQAIEKARQEKLIGNALEAAVVLHCDPRFA
                     TAIPREELEEFFILSDLQLAPAQEPSATVTKTPNKKCARCWRHRPAVGTIAAHPELCD
                     RCAAVVEQI"
BASE COUNT          809 a         1247 c         1402 g          750 t
ORIGIN      
        1 attgtcgagc atgccgtggc tgcccgcgca atcgacccgg tgcagttcga gctcgtccgc
       61 aaaggtcggg cgggccatga agcgtttcag tttcgccacc cgcatgttgg gcacgtcctt
      121 gaagaccatg tgctgtcgga cggcctcgac cgtggcgtcg atctccgcgc gcgaaaagcg
      181 gagccgatgc atgagggcct cggtcatctc cgcaccgacg cgatcgtgcc cgttaaagcg
      241 gatacggccg gcatcgtcga ccgaactggt ggcaggtttg ccgatgtcgt ggaagaggac
      301 gctgagcacg agcggaagcg agacgtcgtt cggcaagagt cccagcatca ggcgcgtgtg
      361 ctggaaaacg tcgccctcgg ggtgaaattg cggcggctgg gcgcagcctt tcatcgccac
      421 gagctcgggc aggacctgtt ccatgagacc actggcatcg agcaggtccc agccgcggac
      481 gcgctgcggc gaaaggaaaa tgcgcaccag ctcgtcacga atccgctcgg cgctgatctc
      541 gccgatcgtc gcggccgccc gcccgactgc ctcccaggtc gccggctcga tttcaaactc
      601 gagcaccgtg gcaaaacgga tggcgcgcaa gaggcgcaaa cggtcctccg cgaaacgttg
      661 cgcggggtcc ccgatcgcgc ggatgacctt cgccgccagg tcctcgcgcc cgccgacgaa
      721 gtcgatcacc tcctcgttag gcgggtcgaa gaagaggccg ttgatcgtga aatcgcggcg
      781 gcgcgcgtcc tcctccgcgg aagaaaaatg cacttcggcc ggacgccgcc cgtcgagata
      841 ggcgccatct gagcgaaagg tcgcgacttc aaactgaaag tcattttcga gcacgacaat
      901 caccccgaaa tgggcgccga cggcgtaggt tcggccaaag attttctgca cctctgcggg
      961 gcgcgcattg gtcgcgatat cgatgtcttg cggcgtcatc ccgcggagga gatcgcgcac
     1021 gcagccgccg gcgaaatagg cgacatggcc ggccgcacgg aggcgttcgg tcagagagcg
     1081 ggcggtcttt tccatctcgc tcatcgcatg caccgcccaa cgtcgccgtt cgtcccgcga
     1141 ctgtcgagag cgacccgacg agcgctagtg gccagtgagt cagatgaaac tgaacggtat
     1201 cgatgcgagc caactggagt cacatcgggc cgagcggagc tcgtcgctcc ggagggactc
     1261 gccacggcga gtccaggacg cgcggaattg cgtcgctcgc ccgattcaga actcaatgac
     1321 tcattgcgct ggtgcaacgt aactttgaaa ttgggcacag cgcgccgtca gctttttttg
     1381 gggagcgcac agcctgctcg ccgtgtgcgg gcgacccgcg tgctggcgat ggcgactcgc
     1441 catcgcggac ttggattgag tttcccataa gagtgcttgg gaggagacca gccgagggag
     1501 aaaagttcgt tgctgcgggt cgcagcaacc agcacgcgag tcgcgtgcgc tccccagaat
     1561 gcacggcctc cgaatctcgc gtggccaatt catcggttac gttgcactag tggcctgctt
     1621 gcgccttcgc cttcgaatcg cggaaggaat agatcatgaa gcaggcgacc gcgaggcaaa
     1681 tgcaggagtc ggccacgtta aaagcaggcc acgggtgggc gaaagggatg tgcaaatcaa
     1741 agagcagaaa atcaatgacg taaccgtgga gcaggcggtc ggtcaggttg ccgaggatgc
     1801 cagccattaa caagccaagc gcgatgcggc gccacggatc gcgcggctcg cggcgcaaca
     1861 tcaggaccgt gaccaccgcc agcgcgatgc aggaaatggc gacgaaaaac cggttgttgt
     1921 cgcggaagga accgaaggct gcgccggtat tggtgacgtg gacgagggta aagaaattcg
     1981 gaatgagatc gcgcggctcg gatgggtcga cgaagtggaa aacgaaatac tttgtgacct
     2041 ggtcgagcgc gtagagcggc aggctgaggt agaagaaaat tttcatttca cgcggcgatt
     2101 cttcttccgg ggagagcgcg cggcgacgcg tcatatttgt tccaccaccg cggcgcagcg
     2161 atcgcataat tccggatgtg cggcgatcgt gccaacggcc ggccgatgcc gccagcagcg
     2221 cgcgcacttc ttgttaggcg ttttggtcac cgtggcgctc ggttcctgcg ccggcgccag
     2281 ttgcaggtcg ctcaggatga agaattcctc gagctcctcc cgcgggatcg cggtcgcgaa
     2341 acgcgggtcg caatgcagca cgaccgccgc ttcgagcgcg ttgccgatca gtttttcctg
     2401 gcgcgctttc tcgatcgcct gaccgatcac gccccggagc cggagtagtt cctcgccttc
     2461 cgtccggagc gcggcatcgc gccacgcggc ttgcggctcc ggaaacaatt gcaaatgcac
     2521 cgatgcgtca tccggcgcgg agcgcagcgg agtcgaggga tgacgatggc tccaggcttc
     2581 ctcggccgta aaaaccagga tgggagccag cagttggcag agcgcatcga aaatatgact
     2641 catcgcggtc tgcgtcgcgc gacggcgggg cgcagcgggc gcgtcgcagt agaggcggtc
     2701 tttggtgatg tcgacgtaga ggctgctcag gtcgaccgcg cagaattggt tgagggtgtg
     2761 gtaaaccttg tggaattcgt aagccgcgta ggccgcgcgg caatcctcca tcacttgctg
     2821 cagccgatcg agaatccagc gatcgacgaa ggtaaactct tccttcctgc tttcctgctt
     2881 tcctgattca aaatcagcga ggttgcctaa caatatccga agcgtgttgc ggatccgccg
     2941 gtaggtatcg ccgaggcggg tgaacatttc ctccgagaac ggcacgtcgt cggtgtagtt
     3001 gatgctgctg acccatagcc tgacgagatc tgcgccgtgc cggccgacga aatggcccgc
     3061 gtccatcggc ttgttgtagg tgcccgactt ggaaatcttt ttcccgtcga catcgaccac
     3121 gaaaccatgc gtcacgcaga tcttgtaggg cgcgcggtcg tgcagcgcga cgctcgtcat
     3181 gagcgacgat tggaaccagc cgcgatgctg atcggtcgcc tcgagataca tgtcggccgg
     3241 gtcgcgcaac gcgggatttg tcgcgcaaac cgcctggtgg gagacgccgc tgtcgatcca
     3301 gacgtcgatc gtgtcgttgc ggtggcgcgt gccggcgggc agcccgagcg ccgcggcgag
     3361 cgccacgtca tcgagttcga accagatgtt ggaaccgcgt tctgcgacga ggtcggccag
     3421 tttgcgaatc cattccgggt tgagcagcga ttcgccggcg gcggaataga aaacaggcag
     3481 cggcacgccc caactgcgct ggcgcgaaat gacccaatcc gggcgtgatt ccaccgtccc
     3541 ggcgatgcga ttctcgcccc acgcggggag ccaggtgacc gtcgtgatcg cgcggagcgc
     3601 gttcttgcgc aggtcatcaa tccggataaa aaactgctcg accgcgcgaa agatgatcgg
     3661 ggttttggag cgccagcagt agggatagga atgccggaag ggctgctcgg cgacgagcat
     3721 gccgcgggcg cgcagcagcc ggacgatgtc ggcgttggcg tcgaaaacat attggccgac
     3781 taattccggc aggcccacct ccgcggtgta gcggccgaga tcgtcgaccg gcgacagaat
     3841 cggcaggccg ttggctttgc ctaacgagta gtcatcctcg ccgtggccgg gcgcgatgtg
     3901 gacagagccg gtgccggtgt ccatcgtgac aaaatccgcg gtcagaacga tcgcggtgcg
     3961 gggtaaaaac gggtgctgcg cgccgatgcc ctcgagtttg ttaccgggga aattttcaag
     4021 cggttcaccg acgggctcga agccggtcgc ggcgctgaac ggcgcgacca ttttgccggc
     4081 gaggacgagc gtttcggaat gtgggagggg cctttgtgcc ccgatcggcg cggggtctgc
     4141 tggggtggct cccgctgatc ggggcacaaa ggcccctccc acgttgaatt tttgcaccac
     4201 gtagcgct
//
LOCUS       DATMNS010000143         7278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4399780, whole genome shotgun sequence.
ACCESSION   DATMNS010000143 DATMNS010000000
VERSION     DATMNS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7278
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4399780"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="VNW28_05045"
     CDS             complement(<1..623)
                     /locus_tag="VNW28_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXA09325.1"
                     /translation="MKRQFNPAVPEWMDRPQPISPELVSDLRNLRALNRYFGSYRLIR
                     HFLARWVRRGNRLRVLDLATGSGDIPRLVVEFARAAGATVAIDAVDFQSSTIEIARRL
                     STDFPEIQYHCADIHQFGEDQSFDIVLFSLALHHFSADHAGQLLRRCRELSRGSVLVA
                     DLRRGWLARLGVDLLTTLVFRQAMTRNDARVSAERAFSFRELDSLARA"
     gene            complement(703..1446)
                     /locus_tag="VNW28_05050"
     CDS             complement(703..1446)
                     /locus_tag="VNW28_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09326.1"
                     /translation="MNQNETDRWNALSVEARAAVTVRDYAARSLGQLIAETGIEAGQL
                     AAASRTAPEVVEAWIPGVEIFARTIYPQRHRGLFGEFARRDEGALARIGLWPRQWSAA
                     RMFAQTAKGFHIHPPSVPGGSSPNEWFQRLFVAEPENFALRRYAEEQWDVMFFVQGRA
                     EMILREARAGLPRRTMRLFLDGDDHRGSNNAAVVIPPGVAHAIRVEGSEDLIMVYGTS
                     TSFQAEFEGRIASEVESAALPESWQKFLS"
     gene            complement(1461..3884)
                     /locus_tag="VNW28_05055"
     CDS             complement(1461..3884)
                     /locus_tag="VNW28_05055"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXA09327.1"
                     /translation="MQRPLFQTIRSDFARLIARKFAPARLLLVGNEAGQITDQRSEDE
                     IESSICPGITELDALRQDGSAPQFDLAIWFYPLEIGSAEDQPILDRLTALVDNLVLIP
                     GAGADVAKRRPPLVMHLASRGFFPDYESDIVDIEPGAIRLSRQKAESVDVLLPAVEIG
                     FARINAQLRRVRRTLRTRMSELEAADRHIARLEEKVLKLKQAKRDLKQLKAEKHALRK
                     SPERKIGQVILAPYRLPQKLIREVRKHLGGPVAPRSPTPSPSEYQKWLQSRRASAKEL
                     AAARAQARAFSYCPLISIITPVFNTPRNWLGEAIDSVVHQAYENWELLLIDDGSTLVE
                     TVDFLATVERHDPRIVLVKRDSTGGISAASNSGLERARGEWVGLLDHDDILEPDALFE
                     VVKYLQTNPETDLIFSDEDKITEEGLAAPQFKPDWSPDFLLSYNYLCHFTTVRREIIE
                     KAGRFRPEFDGAQDYDLFLRVSELTTRIHHLPRILYHWRRSETSTSDNIRRKPQALEA
                     GRHAIESHLERRGERGHVAVDWQTHAYWVKRELRETKRIAIIIATRDRIDLLSRCISS
                     ITSKTSYPNYEIIVVDNESTSDEAREYFRNCEHRVLPFNGPFNFSALNNLAVEQTTSP
                     WLLFLNNDVEAIDNEWLTVMAEHVQRPEVGAVGARLLYPNDTVQHAGVVLGVGGIAEH
                     AFRHFPADAPGVSRQLQVTRNYSSVTGACLLTRRDVFEEVGGFDEERLPVTFNDVDLC
                     LKMRRAGYLIVYTPFARLYHHESASRRRSVEALETEVMRERWPDLLERDPYYNPNLSR
                     ERADFSLGK"
     gene            3899..5878
                     /gene="waaF"
                     /locus_tag="VNW28_05060"
     CDS             3899..5878
                     /gene="waaF"
                     /locus_tag="VNW28_05060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HXA09328.1"
                     /translation="MIAAARRQNYGAAALLPGRRRLPCMVDFCVYLFYRASLTVVTSL
                     PLGFAFSLGRGLGFLAWLLLPNYRRLARRNVEIAFGQEKTAPEKGRIVRRHFQQLGAN
                     LLSGMKLNATPLAEVAKLVATEGVDEVHRELRAGRPVVLVLSHLGNWELFAQILPHYF
                     GYARLSTVYQRLGNRHLDRFVREQRARFGVELFDRSEGFQEAIRLLRAGGMIGILSDQ
                     HAGDHGMWTPFFGRLASTSPLPGLLCKRTGAALIAASLYTAGTGKWRMTFSRSGDLPN
                     DSVQSLTAKANETIAEEIRRAPEDWFWVHNRWKTPRPNWLLSKYKRGCFIPETVSAEG
                     LKPFRILIRGSNWLGDSVITIPAIRALKHGRPDAHVTVFAPEKIAAVWRLVPEVDVVV
                     SLGKKSLWHAVRRLRREPGFDAGIVFPNSLRGALELWLGGIPRRVGYAGHHRRWLLNQ
                     LVPKIETKGPPPHQSEHYLDLVRSLGAEAAVKEIALATGLATTSAGLKLGLCPGAEYG
                     PAKRWLAERFGATAAAVGGQWVLLGTGKDEEVGAQIAAELGAKCTNLIGKTTLEQLID
                     ELRECQLLLTNDTGTMHLATLLGVPVVAVFGSTEPSLTGPLGQKSRVVRHQVECSPCF
                     LRECPLDFRCMKAVSVEEVVALVRESRIENRDSEE"
     gene            complement(5894..6454)
                     /gene="frr"
                     /locus_tag="VNW28_05065"
     CDS             complement(5894..6454)
                     /gene="frr"
                     /locus_tag="VNW28_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXA09329.1"
                     /translation="MSAEEILLEAEMAMEKSVDYMIHEFASVRTGKASPALVENVDVE
                     AYGSAMKLKQLALITTPEPRLLVVQPFDASTVRDIEKALKESKIGIMPLVDGKIIRLP
                     IPELSEERRKDLVRSLKQMAEEARVRVRANRRTAMEEAKKMEKAGEATEDQLRDTEAE
                     IQKLTDRFVKSIDEHLERKDAEVMKV"
     gene            complement(6497..7222)
                     /gene="pyrH"
                     /locus_tag="VNW28_05070"
     CDS             complement(6497..7222)
                     /gene="pyrH"
                     /locus_tag="VNW28_05070"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXA09330.1"
                     /translation="MKTEAPAYKRVVLKLSGEALQERGARENISPAVVREIAERIKEV
                     NQLGVQIAIVIGGGNIWRGSSAAVRGMDRTTADYMGMLATVINGLALQSELEQSGVIT
                     RVQTAIEMQNVAEPFILRRAIRHLEQGRVVIFVAGTGNPYFSTDTTAALRASEIDADV
                     ILKATKVDGIYDRDPKIHPDAKRYERISFTEALTKRLQVMDSTAFSLCMDNKIPIIVF
                     DMHQPTNVRDVVLGKKIGTLVSD"
BASE COUNT         1355 a         2170 c         2286 g         1467 t
ORIGIN      
        1 gcccgcgcga gagagtccag ctcgcgaaac gagaaagcgc gttccgccga gacgcgagca
       61 tcattgcgag tcatcgcctg gcgaaaaacg agcgtcgtga gcaaatccac gcccagcctg
      121 gccagccagc cgcggcgcaa atcggccacc aggacggacc ctcgggaaag ctcgcggcag
      181 cggcgcagta attggccggc gtggtcggcg ctgaagtgat gcaaggcgag cgaaaaaagc
      241 acgatgtcaa aggactggtc ttcaccaaac tggtgaatat ccgcgcagtg atactgaatc
      301 tccggaaagt ccgtgcttag ccggcgggca atctcgatag tcgaagactg gaaatccacc
      361 gcgtcgattg ccaccgttgc gccagctgca cgagcgaact cgacaaccag ccgcgggatg
      421 tctcccgagc cggttgccag atcgagaaca cgcagcctgt tgccgcgtcg tacccaacgc
      481 gccaaaaagt ggcggatcaa ccgatagctc ccgaagtagc ggttcagcgc gcgcagattc
      541 cgcagatcgg aaaccagttc cggcgagatc ggctgcggcc ggtccatcca ttccggcaca
      601 gccggattga actgccgctt caaggcggga ggggcctttg tgttccgatc cgggggcgcg
      661 ccgcaacccg tgggggcacc aaggcccctc ctcctaccga attcatgaga gaaatttctg
      721 ccacgactct ggcagggcgg ccgactcgac ctcgctcgcg atgcgacctt caaactcggc
      781 ttggaatgaa gtgctggttc catagaccat gatcaaatct tccgaccctt cgacccggat
      841 ggcgtgggcc acgcctggcg gaatgaccac cgcggcattg ttcgacccgc gatggtcgtc
      901 gccatcgagg aaaaggcgca tcgtccgccg cggcaaaccg gcgcgcgctt ctcgcaggat
      961 catttcggcg cgtccctgga caaaaaacat cacgtcccat tgctcctcgg cgtagcggcg
     1021 gagcgcgaag ttttccggtt ccgcaacgaa aagacgctga aaccattcgt taggcgaact
     1081 gcctcccggc acgctcggcg ggtgaatatg aaaacccttc gccgtttgcg cgaacatccg
     1141 ggcggccgac cactggcggg gccagaggcc gatcctggcg agagcgcctt cgtcgcgccg
     1201 cgcaaattcc ccaaacaatc cgcgatgccg ttgcggataa atcgtccgcg cgaagatttc
     1261 gacgcccgga atccaggcct ccaccacttc cggggcggtg cggctggcag ccgcgagctg
     1321 gccggcttcg atgccggtct ccgcgatgag ctggccgagg gagcgggctg cgtaatcgcg
     1381 caccgtgaca gccgcccgcg cttcgacgga gagtgcgttc cagcgatcag tctcgttctg
     1441 gttcatcgga aaatgaagag ctatttgcct aacgaaaaat cggcgcgttc ccgcgaaagg
     1501 ttcggattgt agtagggatc gcgctccagc aaatcgggcc agcgctcccg cattacttcg
     1561 gtctcgagcg cttccacact gcgccgccgc gaggccgatt cgtggtgata cagcctggcg
     1621 aagggcgtgt aaacaatcag ataaccggcg cgccgcattt tcaggcaaag gtcgacgtcg
     1681 ttaaaggtga ccgggaggcg ttcctcgtcg aacccgccga cctcctcgaa gacgtcgcgc
     1741 cgggtgagca ggcacgcgcc cgtgaccgaa ctgtagttgc gcgtcacctg cagctggcgc
     1801 gagaccccgg gcgcatcggc gggaaaatga cggaaggcat gttccgcgat gccaccgacg
     1861 ccgaggacca ccccggcatg ttgtaccgtg tcgttagggt aaagcagccg cgcgccgacc
     1921 gccccgacct caggccgctg cacatgttct gccatcacgg tgagccattc gttgtcgatc
     1981 gcttcgacgt cgttatttaa aaagagcagc catgggctgg tcgtttgttc cacggcgaga
     2041 ttgttcagcg ccgagaaatt gaacggtccg ttgaagggca ggacgcgatg ctcgcaattg
     2101 cggaaatatt cacgcgcctc atccgacgtg ctctcgttgt cgaccacgat gatctcgtaa
     2161 ttcggatagc tcgtcttgga ggtgatgctg gagatgcagc gggagagaag gtcgatccgg
     2221 tcgcgggtcg cgatgataat cgcgatccgt ttcgtctcgc gcagctcgcg ctttacccag
     2281 taggcgtgcg tctgccagtc caccgccaca tggccgcgtt cgccgcgccg ttcgagatga
     2341 ctttcgatcg catgccggcc cgcttcgagg gcctgcggtt tgcgccggat gttgtcggag
     2401 gtggaggttt cgctgcggcg ccagtgatag aggatgcgtg gcagatgatg aatgcgcgtc
     2461 gtcaactcgc tgacacggag aaaaagatcg taatcctgcg cgccatcaaa ctccggccga
     2521 aaacgccccg ccttttctat gatttcgcgc cggacggtgg tgaaatggca gaggtaattg
     2581 taggaaagca aaaagtccgg cgaccaatcc ggtttgaact gcggggccgc cagtccctcc
     2641 tccgtaattt tatcttcgtc ggaaaagatg agatcagtct ccgggttggt ttgcagatac
     2701 ttgaccacct cgaagagcgc gtccggctcg agaatatcgt cgtgatcgag gaggccgacc
     2761 cactcgccgc gggcgcgctc cagtccggag ttggaagcgg ccgaaatccc gccagtgctg
     2821 tcccgtttca cgaggacgat ccgcggatcg tggcgctcga ctgtggccag aaaatcgact
     2881 gtttccacca gcgtgcttcc gtcgtcgatc aggagcagct cccagttttc gtaggcctgg
     2941 tgaacgaccg aatcgatcgc ctcgcctaac caattgcgtg gtgtattgaa gaccggggtg
     3001 atgatgctga tcaggggaca ataagaaaaa gcgcgggctt gggcgcgcgc cgcggcgagc
     3061 tctttggccg acgcccgccg gctctgcagc catttttgat attcgctagg cgagggagtg
     3121 gggcttcgcg gcgccaccgg gccgcccaaa tgtttgcgca cttcgcggat caatttttgc
     3181 gggaggcggt agggcgcgag gatgacctgc ccgatcttgc gctcgggtga tttccgcagg
     3241 gcatgctttt ccgccttgag ttgtttcaaa tcgcgcttcg cctgtttgag cttgagcact
     3301 ttctcttcga gccgcgcgat gtggcggtca gctgcttcca gttccgacat ccgggtgcgc
     3361 aaagtgcggc gcacccggcg cagctgggcg ttgatccgcg cgaagccgat ctccaccgcc
     3421 ggcaggagca catcgacgga ttcggctttt tgccggctca agcggatggc tcccggctcg
     3481 atatcgacaa tatcggactc gtaatccggg aagaagccgc ggctggccag gtgcatgacg
     3541 agcggtggcc gccgcttcgc cacgtcggct cccgcgccgg gaatgaggac gagattatcc
     3601 accagcgcgg tcagccgatc gaggatcggt tggtcttccg cggacccgat ttccagcggg
     3661 taaaaccaaa tggccaggtc gaactgggga gcgcttccgt cttgccggag ggcatcgagc
     3721 tcggtgatcc cggggcagat ggagctctcg atctcgtctt cggaacgttg atcggtgatt
     3781 tggcccgcct cgtttccgac gaggagcaac ctggccggcg cgaattttcg ggcgatgagg
     3841 cgcgcgaaat cggaccggat cgtttgaaaa agaggtcgct gcacgggagc acattataat
     3901 gattgcagcc gcccgcaggc aaaattacgg tgctgccgca ctccttcccg gccggcgccg
     3961 gctgccctgc atggtcgatt tttgcgttta cctcttctat cgcgcgtcgc tgactgtggt
     4021 tacctcgctg ccgctcggct tcgccttttc cctcgggcgg ggactcggtt tccttgcctg
     4081 gttgctgctg ccgaattatc gccggctcgc gcgccgcaac gtcgagatcg cgtttggcca
     4141 ggagaaaact gcgccggaga aagggcgaat tgtccgccgc cattttcaac agctcggcgc
     4201 caatctgctc agcgggatga agctcaacgc gacgccgctc gcggaagtgg ccaagctggt
     4261 cgcgaccgag ggcgtcgacg aagtgcaccg cgagctgcgg gcgggccggc cggtcgtgct
     4321 cgtgctcagt catctcggga attgggaatt gttcgcccaa atcctgccgc attacttcgg
     4381 ttacgcgcgg ttgagtaccg tttaccagag gctgggcaac cgccatctcg accggtttgt
     4441 gagggagcag cgcgcccgct ttggggtcga gctctttgac cgaagcgaag gctttcagga
     4501 ggcgatccgg ctgctccggg ccggcggcat gatcgggatc ctgagcgacc agcacgcggg
     4561 tgaccatggg atgtggacgc cgttcttcgg ccggctcgct tcgacttcgc ccctgcccgg
     4621 tttgctttgc aaacgaaccg gtgcggcctt gatcgccgcc tccctttaca ccgccggcac
     4681 ggggaaatgg cggatgactt ttagccggtc gggtgatttg cctaacgata gcgtgcagtc
     4741 gcttaccgcg aaggcgaatg agacgatagc cgaggaaatc cggcgcgcgc cggaggattg
     4801 gttctgggtc cacaatcgct ggaagacgcc gcggccaaac tggctcctaa gcaaatataa
     4861 gcgcggttgt tttatcccgg aaaccgtcag cgcggagggc ctgaagccat ttcgcattct
     4921 cattcggggc agcaactggc tgggggacag cgtgatcacc attcctgcga ttcgcgcgct
     4981 caaacacggc cggcccgacg cgcacgtcac cgtcttcgcg ccggaaaaga tcgctgccgt
     5041 ctggcgactc gtcccggaag ttgatgtcgt agtttcgtta ggcaaaaaat cgctctggca
     5101 tgccgtccgg cggttgcgcc gcgaaccggg atttgacgcc gggattgttt tccccaactc
     5161 gctgcgcggg gcgctcgagc tttggctggg cggtattccc cgccgggttg gttacgccgg
     5221 gcatcaccgg cgatggctct tgaaccagct ggtcccgaag atcgagacga agggaccacc
     5281 accgcaccag tcggagcatt atctcgacct cgtccgttcg ttaggggcgg aggccgcggt
     5341 caaggagatt gcgcttgcca ccgggttggc aacgacgagc gctgggttaa aactgggcct
     5401 ttgccccggt gcggaatacg gcccggcgaa acgttggttg gcggaacgat ttggggccac
     5461 ggcggcggcg gtcggcggcc aatgggtcct cttgggcacg ggcaaagatg aggaggtggg
     5521 cgcgcagatc gccgcggagc tcggtgccaa atgcaccaat ctcatcggta aaacgactct
     5581 cgagcaactg atcgatgaac tgcgggaatg ccagcttctc ctgacgaacg ataccggcac
     5641 aatgcatctc gcgaccctgc tcggcgtccc ggtcgtggcc gtttttggtt cgacggagcc
     5701 gagtctgacg ggaccgcttg ggcagaaaag ccgggtcgtt aggcaccagg tcgagtgcag
     5761 cccgtgtttc ctgcgcgagt gcccgctcga tttccgctgc atgaaagcgg tcagcgtcga
     5821 agaagtggtc gcgttagtcc gagaatcgag aatcgagaat cgagattcgg aagagtaggc
     5881 gattcgcaga ttctcacacc ttcatcacct cggcgtcttt gcgctcgagg tgttcgtcga
     5941 tcgatttcac gaaacgatcg gtcaatttct ggatctccgc ctcggtatcg cgcaactggt
     6001 cttccgtcgc ttcgccggcc ttttccatct ttttggcctc ctccatcgcg gtccggcgat
     6061 tggcccggac gcggacgcgc gcttcctcgg ccatttgctt cagcgagcgc acgagatcct
     6121 tgcgccgctc ctccgagagc tcgggaatcg gcaggcggat gatcttgcca tcgaccagcg
     6181 gcatgatgcc gatcttggat tctttcagcg ccttttcgat gtcgcgcacg gtcgaggcgt
     6241 cgaatggctg gacgacgagc aagcgcggtt ccggagtggt gatgagggcg agttgcttga
     6301 gtttcatcgc cgagccatag gcttcgacat caacgttctc aaccagggcg ggcgaggcct
     6361 ttccggtccg cacgctggca aactcgtgga tcatgtagtc cacgcttttt tccatcgcca
     6421 tctcggcttc gaggaggatt tcttcggcac tcataaggct ggtgaatgaa agctgcggcg
     6481 gcccgaatcg caatcgttaa tcggagacca gcgttccgat ttttttcccg agcacgacat
     6541 cgcgcacgtt agtcggctgg tgcatgtcga aaacaataat cggtatcttg ttgtccatgc
     6601 aaagactaaa ggcggtcgag tccattacct gcaaccgttt cgttagggcc tcggtgaagc
     6661 tgatccgttc atagcgtttc gcatccggat ggattttggg atcacgatcg tagataccgt
     6721 cgactttggt cgccttcagg atgacgtccg cgtcaatttc gctggcccgt aaggcggctg
     6781 tcgtgtcggt cgaaaaatag gggttgcccg tgccggcgac gaaaataacg acgcgaccct
     6841 gttcgagatg ccgaatggcg cgccggagga tgaaaggctc ggcgacgttt tgcatttcga
     6901 tcgcggtctg cactcgcgtg atgacgccgc tttgttcgag ttcactttgg agtgcgaggc
     6961 cgttgatcac agtggcgagc attcccatgt aatcggcggt cgtgcgatcc attccccgaa
     7021 ccgccgccga cgaaccgcgc cagatgtttc cgccgccgat cacgatcgcg atttggacgc
     7081 cgagctggtt gacttccttg atccgctcgg cgatttcccg gaccactgcc ggggaaatat
     7141 tttcgcgcgc cccccgttcc tgaagggctt caccgctgag cttgagcaca acgcgtttat
     7201 aggccggcgc ttcggtcttc atcgctggtc aaatgaaacg cgaggatttc cctcttggaa
     7261 agtcaaaacg cgtcttgg
//
LOCUS       DATMNS010000144         4583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4853147, whole genome shotgun sequence.
ACCESSION   DATMNS010000144 DATMNS010000000
VERSION     DATMNS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4853147"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VNW28_05075"
     CDS             <1..194
                     /locus_tag="VNW28_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09331.1"
                     /translation="HSCAITLLAAGKFINGIEGRAQPIVIQAPRVVPLAKLPILSPVW
                     VPTLFLEACPCEHGPPALS"
     gene            477..1235
                     /locus_tag="VNW28_05080"
     CDS             477..1235
                     /locus_tag="VNW28_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09332.1"
                     /translation="MPPGLLTDTTQEGRFVSTRWTVVIAAGESGFSSGHGLDALSELC
                     RIYWRPLYLFLRREGIGPEDAQDLTQGFFAELIRDRAYLRADRAKGRFRSFLLGALKH
                     FVWEMRDRERAQKRGGGKISEPLDERAIAEVEAQVASARNWAADSLYDREWAAALLRQ
                     TLDRLRQECVIAGKTALFDSVQPYLSAARDKVIPYDEISRRLNRNIGTLRSDIARLRL
                     RYRAILREEVRGTIAEPTEVDEELRYLCQVIARA"
     gene            1340..4147
                     /locus_tag="VNW28_05085"
     CDS             1340..4147
                     /locus_tag="VNW28_05085"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXA09333.1"
                     /translation="MIGEKTNPTVCEVCGSRIESTASGEFGCLVCLLRDGLGEPTEAP
                     EGWSDAPPLSLGQYVIMRREDGTLWELGRGAMGVTYRAQDPSLQREVALKIIKTDLIG
                     PISEARERFMREARAAAALRHPSVATVYQFGIDEASGQCFCAMELVEGETLDERVRRT
                     GPLDVPSVIEIAQQITAALTAAERHGLVHRDLKPANVMIVEGGEPGGLEVKIIDFGLA
                     KALGATTETRDLTHGGFVGTPAFASPEQLRDAPVDVRSDIYSLGAMLWYLLSGHRPFG
                     QGAPAQAPVEQLKVAQVPSRLATLLVSMVAPEPAVRPGTQELAKKLRALNAQVSSSHW
                     WNRKKSVALMVVLVLLTAAFLLFVIQRERSGKVPEKSLAVLPFENESDDRQNTYFADG
                     MQDDVMTGLAQLADLRVINRASVQNYRDPAKRPSARAIGEALGVAYLVEGSVRRQGDR
                     VRINARLIQAASERQVWAADYDRQLSDVFAIQSEVARQIADELQLRLSATEKQRIDEP
                     PTRDFAAYELYLHARELLDNYVEGSDQWQPLNEAVRLLNEATARDPQFALAWCGLASA
                     HSQLYQFKADHTPARLSLAGEAVQKALALRPDLGEPHLAAAIYFSLEHRDDVRGRAEL
                     GLARAKLPNNAHVALLSGKIAQRDGRWAEMLAELERSHTLNPRDRAAVDELGTVYDAL
                     RRYDEARRVLDQATAAGLNSDWFATRRAAFFWHDKGDTSELRTVFQRRLALGTPNERL
                     FAARIQTAWCDGNFDEAARLLAADPRQQFEAGEKRFYPRAYFEGMTARARGDQLAARI
                     AFTAARPFLEETFRQQPDNPNCLIILAEVDAALGNKEQALAEANRAVAMQEKAKNAWE
                     GSVMLVGRAQVYAWAGEHQQAIAQLESLVNRPRVCHYGYLKMDPDWVDLRGEARFQRL
                     VESLAPARGQSRIIPVDQTTQ"
     gene            4316..>4583
                     /locus_tag="VNW28_05090"
     CDS             4316..>4583
                     /locus_tag="VNW28_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09334.1"
                     /translation="MKSRFRILLAATIFFAGLALLFAALALPGQVSAQDKPDHNNKHH
                     HYKFIDLGTFGGPASFIGTPNNVNPELSRQGTTGGASATSVSLTS"
BASE COUNT          934 a         1355 c         1403 g          891 t
ORIGIN      
        1 gccattcctg cgcgattacg ctgctcgccg cgggcaaatt cattaatggg atcgaaggtc
       61 gcgcacagcc gatcgttatc caagcgccaa gggtcgtccc actggcgaag ctgccgatcc
      121 taagtccggt ctgggttcca actcttttcc tggaagcctg cccctgcgag cacgggccgc
      181 cggcgctttc ctagcgcttg tctgaggcca cttttcccac ttactccggg ataagtctcg
      241 cctgccatac gtttggccgt ctggccctgc gatgcgcgtg ttaccaacgt cgaacgatct
      301 ttaattagca atacgaccct gagaaaccaa agcattgagg cttattcact cgattgccac
      361 caggacatga aggaaccgac caatagttac aaatcagatc atcaaccaaa ggagaatcgc
      421 atgagtcacg aatcagaaaa aaacagaaat ccaaaaccgc cgcctagaaa cgccgaatgc
      481 ctcccggatt gttgaccgac acgacgcagg agggacgatt tgtttctacc cgctggacgg
      541 ttgttatcgc ggcgggcgaa tctggatttt cttccgggca tgggctcgac gcactctcag
      601 agctttgccg aatttactgg cggccgcttt atctttttct gcgccgagaa ggaatcggac
      661 ccgaggatgc gcaggacctg actcagggat ttttcgccga gctcatccgt gatcgggctt
      721 acctgcgcgc cgatcgcgcc aaagggcggt tccgctcctt tctcctcggt gcgttgaagc
      781 atttcgtctg ggaaatgcgc gatcgcgaac gcgcccaaaa gcgcggtggc gggaaaatta
      841 gcgagccatt ggacgagcgg gccattgccg aagtagaagc acaagtcgcg tccgccagga
      901 actgggccgc ggattctctt tacgaccgcg aatgggccgc cgcgcttctc cggcagacgt
      961 tggatcgcct ccggcaggaa tgcgtgatcg cgggcaaaac cgcgctcttc gacagcgtcc
     1021 agccttacct gtcagcggcg cgcgacaaag tgatccctta tgatgagatt tcgcgacgat
     1081 tgaatcgcaa catcggaacc ttgcgcagcg atatcgcccg cttgcgtttg cgttatcgcg
     1141 cgattctgcg ggaggaagtg cgtggcacga ttgccgagcc gacggaggtg gacgaggagc
     1201 tgcgttatct ctgccaggtg attgcccggg cctaacgaac aaaaatggaa tctcatgaac
     1261 cgggatctta aacacgaatt cagcgttcaa gtagccgcga cgaccgcaga ccgcggccac
     1321 cataagtctg agacactgga tgattggcga aaaaactaac ccgacggtct gcgaagtttg
     1381 cggcagtcgg attgagagca ccgcctcggg cgagttcgga tgcctggttt gtctgctgcg
     1441 cgatggactg ggcgaaccga ccgaggcgcc cgagggttgg tcagacgccc cccctttgtc
     1501 gttaggccaa tatgtgatca tgcggcgcga ggatggcacg ctttgggagc ttggtcgcgg
     1561 cgcgatgggc gtgacttacc gcgctcagga cccctcgctc cagcgggaag tcgcgctcaa
     1621 aatcatcaag accgacttga tcgggccgat ctcggaggcg cgggagcggt ttatgcgcga
     1681 agcgcgcgcg gcagcggcgc tccggcaccc gagcgtggcc acggtttacc aatttgggat
     1741 cgacgaagcg agcgggcaat gcttttgcgc gatggaactg gtcgagggcg aaacgctcga
     1801 cgaacgggtg cggcgaaccg gtccattgga cgtgccgagt gtgatcgaga tcgcgcagca
     1861 aatcacggcc gcgctcaccg ccgcggaaag gcatggcctg gtccatcgcg atttgaagcc
     1921 cgccaacgtg atgatcgtgg agggtggaga acccggcggg ctcgaggtca agatcatcga
     1981 cttcggtttg gcgaaggcgt taggcgccac caccgagacc cgggacctga ctcacggggg
     2041 ttttgtcggg acgccggctt tcgcgagtcc cgagcaattg cgcgacgcgc ccgtggatgt
     2101 gcgctcagac atttactcgt tgggggcgat gctttggtat ctcctgagcg gccatcgtcc
     2161 cttcggtcag ggcgctcccg cccaagcgcc cgtcgaacaa ctgaaagtgg cccaggttcc
     2221 gtcccggctc gcgacgctcc ttgtttccat ggtcgcacca gagccggcgg tccgaccggg
     2281 aacgcaggaa ctggcgaaga aattgcgggc gctcaacgct caggtttcca gctcgcactg
     2341 gtggaaccgg aagaaaagcg tcgccttgat ggttgttctg gtccttctga ccgcagcttt
     2401 tctcctcttc gtgatccagc gcgagcgatc aggcaaagtg ccggaaaaaa gcctggccgt
     2461 gctgcctttc gagaatgaaa gcgacgatcg gcagaacact tattttgcgg atgggatgca
     2521 ggacgatgtc atgaccggcc tcgcccaact ggctgatcta agagtcatca accgggcgag
     2581 cgtgcagaat tatcgggacc cggccaaacg cccgagcgcg cgcgcgatcg gggaagcact
     2641 gggcgtggct tacctggtgg aaggaagtgt ccgccgtcag ggggaccggg tgcgaatcaa
     2701 tgcgcggctg attcaggcgg ctagtgaacg ccaggtctgg gcggcggatt acgaccggca
     2761 gttgtctgat gtgtttgcca ttcaaagcga agtcgcaaga cagatcgccg atgagctgca
     2821 acttcgctta tcggccacgg agaagcagcg catcgatgaa cctccgaccc gagactttgc
     2881 cgcttacgaa ttgtatctcc acgcccgcga gctgctggat aattacgtcg aaggcagcga
     2941 ccaatggcag ccgttgaacg aagccgtgcg tttactgaac gaggcaacgg cgcgcgatcc
     3001 ccagttcgca ctcgcctggt gcggcctggc ctcggcgcac tctcaactct accagtttaa
     3061 agccgatcat acgccggcgc gcctttcgct cgctggagag gcagtgcaga aggccctggc
     3121 cctccggccc gatcttggtg aaccacatct cgcggcggcg atttattttt cgctcgaaca
     3181 tcgagacgat gtgcgcggcc gcgcggaact cggcctcgcg cgggcgaagc ttcccaacaa
     3241 tgctcacgtc gctctgttga gcggcaagat cgcgcagcgc gacggtcgct gggcggagat
     3301 gctggccgag ctggagagat cgcacactct taacccgcgc gaccgggcgg cggtggacga
     3361 gctcggcacc gtttatgatg cattacgtcg ctacgacgag gcgcggcggg tgcttgatca
     3421 agcgaccgcg gccggtctga attcggactg gttcgcgacg cgccgagccg ccttcttttg
     3481 gcacgataaa ggagacacgt ccgaacttcg cactgtcttc cagcggcgcc tggccctggg
     3541 cacgcctaac gaacggcttt ttgccgcgcg catccaaacc gcgtggtgcg acggtaactt
     3601 cgatgaggcg gcgcgtctcc tcgcggctga tccgcgtcag caattcgagg ccggcgagaa
     3661 gcggttttat ccccgagcct acttcgaagg catgaccgcc cgcgcgcgtg gcgaccagtt
     3721 agccgcccgc attgccttta cggccgcgcg tccgtttctg gaggaaacct ttcggcagca
     3781 acctgacaac ccgaattgcc tgatcatcct ggctgaggtt gatgcggctt tgggtaacaa
     3841 ggagcaggcc ctggctgagg ccaatcgcgc ggtagcgatg caggagaagg caaagaacgc
     3901 ctgggaaggt tccgtcatgc tcgtggggcg ggcccaagtc tatgcctggg ccggcgaaca
     3961 ccagcaggcc attgcacagt tggagagttt ggtgaatcgg ccacgcgtct gtcactacgg
     4021 ctatcttaag atggacccag actgggttga ccttcgtggc gaagcgcgct tccagcgcct
     4081 ggtcgaatcc ctcgcgcccg ctcgcggcca gagtcgaatc attcccgtcg accagactac
     4141 ccagtaggaa gttccttatg cactcgagcg gaaatcaggc gtctggcctg acccggcgcg
     4201 cagcgccttc cgggctacac gagtcgcagc ccgtgttttt cgacgttttc aaaaaatatt
     4261 ctgctacacc ggcgaagatt ttcccaagga ataaacagga aacacaatac gaaaaatgaa
     4321 atccagattc cgaatattac ttgctgcaac catctttttc gccgggctgg cattgctttt
     4381 tgccgcgctg gctttgccgg gccaggtctc cgcgcaggac aagccggacc acaacaacaa
     4441 gcaccatcat tacaagttca tcgacctggg tacgttcggt gggcccgcga gtttcattgg
     4501 cacaccaaat aatgtaaacc cagagctgag caggcaggga acgaccggag gggcctcggc
     4561 gacatccgtt tccctcacat cga
//
LOCUS       DATMNS010000145         7829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1630750, whole genome shotgun sequence.
ACCESSION   DATMNS010000145 DATMNS010000000
VERSION     DATMNS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1630750"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VNW28_05095"
     CDS             complement(<1..1423)
                     /locus_tag="VNW28_05095"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HXA09335.1"
                     /translation="MILRGRFVRLVLCGNLALVVLRPLLAEEPKINWQPWTDSIFEQA
                     SREHRFVLLDLEAVWCHWCHVMDAQTYADPKVVALIQSHYLAVKVDQDSRPDIAARYE
                     DYGWPATVVFNSDGSEIVKRQGYLPPKLMASMLQAIIDDPSPGPSIVNEPPIIPGSDA
                     GLTPEARQKLEQVLRDDYDKKNRGWGTVQKFLDWDVIEFCLGQTLAGDREFERMARET
                     LDAQLQLIDPVWGGVYQYSTDGDWKHPHFEKIMQFQAENLRLYAEAYALWKDPKYLEA
                     ATRIRGYLRDFLTSPEGAFYTSQDADLVPGEHSADYFALRDGARRARGVPRVDRHIYA
                     RENGWAINALATFSGVTGDAAALADAVRAAEWIIAHRSLPGGGFRHDEKDAGGPYFGD
                     NVYLARAFLTLYEVTADRKWLQRAEETAQFAADHFGGGVGYVAVANSLGKKWPAKPQT
                     DENTAFARLTNQLSYYGGRAANHA"
     gene            complement(1420..1899)
                     /locus_tag="VNW28_05100"
     CDS             complement(1420..1899)
                     /locus_tag="VNW28_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXA09336.1"
                     /translation="MMRLAEKFYRGLIAFANLLRSPLLLLTRLYWGCQFFLSGQGKLM
                     DLSKPTQYFESLGIPFPHAQAILAACTECFGGLLLLVGLATRLISVPLMILLTTAYLT
                     ADFDRVRVIFMDPDKFVTADEFLFLFAVVILFVFGPGAFSADALIGGYFKRHEGKPA"
     gene            complement(1896..2843)
                     /locus_tag="VNW28_05105"
     CDS             complement(1896..2843)
                     /locus_tag="VNW28_05105"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HXA09337.1"
                     /translation="MSKNNSKPRRPNNPVASRPQLKELQTLMASAIFRPLTARDGMQT
                     RWTDGRSMRAVAQRFIKPNDRLTSFERLEIYNRVYWFRVLDCLYDDYPGLRAIVGEKK
                     FMKLATAYLAKYPSASFTLRNLGQRLEQFLREEPQWIAPHEELALDMAKFEWAQVVAF
                     DDAARPQLTTDDLLDTPPARLRLGLQPYLSLLELNYAVDKFLLVVKKREADSLRGEAS
                     NTPDAMPAAPVRKRRMRLPKHERVYLVVHRYDNMLYYKRLEPEGFAILQALQRGKNVE
                     KACLAALSESARSDVDWTRQIQEWFHDWSALGWFCRPRK"
     gene            complement(2809..3660)
                     /locus_tag="VNW28_05110"
     CDS             complement(2809..3660)
                     /locus_tag="VNW28_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HXA09338.1"
                     /translation="MPANAFNNFSDYGIGIGLRVPHYRHILEKKPVVDWFEIISENFM
                     VDGGRPLEVLDQILEQYRVVQHGVAMYLGSADRLSREHLKKIKRLVKRTNTPWLSDHL
                     CWGSVDGRYTHDLLPMPYTFAAARATAANIREAADFLEVPICVENVSSYAEFHASEMT
                     EWEFLSEVVERANCGILLDVNNIYVSSRNHNFDPYEYLNNVPHHRVGQIHVAGHSKFE
                     KYILDTHDHPVLDPVWKLYAHAIKLVGRTATLLEWDDRIPTFDEVHHEALKANQFVEK
                     QFQTAAA"
     gene            complement(3747..4028)
                     /locus_tag="VNW28_05115"
     CDS             complement(3747..4028)
                     /locus_tag="VNW28_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09339.1"
                     /translation="MTSKAILAGAAIAGLMSGSFAVQTHAANVSTKAGVSVSQMAKKK
                     DVHSCKGQNSCKGKGGCKTGDNGCKGKNSCKGKGGCATDGSHPAASPAN"
     gene            complement(4277..5170)
                     /locus_tag="VNW28_05120"
     CDS             complement(4277..5170)
                     /locus_tag="VNW28_05120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09340.1"
                     /translation="MFLICLFLSGGIFLSEASTGPIKMGGGRIDVVFLGNDPGFPRAL
                     ALEWINTAARAVTKYYGRYPVSHAVIRVRLVGGDRIDSGRTIGTSDGGLITVVVGRST
                     SKAHFDADWLMTHEMVHLALPSVPEKNHWIEEGLATYVEPIARVRIGTLSARQVWSDL
                     VRDLPQGLPGPGDRGLDLTPTWGRTYWGGALFCLLADVEIRRRTANAKGLEDALRGIL
                     EAGGTIASDWDIERVLETGDRATGVPVLRELYSEMRASPHPVNLDQLWKELGIERRGA
                     TVALDDGAPLAAVRKGILPER"
     gene            complement(5315..5800)
                     /locus_tag="VNW28_05125"
     CDS             complement(5315..5800)
                     /locus_tag="VNW28_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09341.1"
                     /translation="MPHSTKETRRPAPNDGSSGPVRIVPGRYKTHASVLFPFKVDAKI
                     AFGSRAADELIAVSAAAAAVAVAREHRHDGDRSGQTYHGRIVSIKDGLVHISELADFG
                     VNQHGKCRENWRDDGGGEVRGNYRSEGGRGRERRRDEPRPVSDQAQARPQSKSEDYES
                     S"
     gene            5889..7589
                     /locus_tag="VNW28_05130"
     CDS             5889..7589
                     /locus_tag="VNW28_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09342.1"
                     /translation="MTLQRLERTYVRLALLVLFGIVLFVGLCWAGSHAYVRWQEHSLM
                     RQAHAALQRGDLRWASLAAQRAYGVNSSSLDACRTLAEIAEKGQDEGAIDWRRRVVEI
                     DPHSLFNQLALATTALRFAKPAIAADALAAVPAEKRNDDGYQAVAARVSLTKNDLVAT
                     RVHLAAAVRLAPNNPDHQLALAQFDLASKDDVQRAEGRKLAELLKTKPTVRREALVLL
                     INDALRRRSLSEATKLTNELAALPNASFADEILTASFQHTVNEPTFIASVTRLEEQAA
                     SSPEKAAQLIAWMNTEALSLLAIDWSKKLPANLLDNLAVRVAVGDSYAQSGNWKGLED
                     ILQHGTWTGAESLNLAWRAKVADESSDAVGREKFWTEAVAKARGQSEQSNLLARLALQ
                     WHWPEKATAVLWTCSEQRETQLPALKALYLYYTEQRDTAGTYRVLGKLVELLPDDLSI
                     RNNFILISLLLQTEPTRMRTMARELNQKEPGNGSFASTYAFALYQTGDTAGALRVFNT
                     LSPEQLRDPAIALYYGVILAGAGRRHEAAAALAKTGGAKLLPEERALVESVRAKLAQQ
                     "
BASE COUNT         1619 a         2339 c         2330 g         1541 t
ORIGIN      
        1 gcgcgtggtt ggcggcgcgg ccgccgtagt aactcagctg attcgttagg cgagcgaagg
       61 cggtgttttc atccgtctgc ggtttcgccg gccacttttt gcctaacgaa ttagcaacgg
      121 cgacgtaacc gacgccgccc ccgaaatgat cggcggcgaa ctgcgctgtt tcttccgcgc
      181 gttgcagcca tttccgatcg gccgttacct cgtaaagggt caggaaggcg cgtgccagat
      241 agacattgtc gccgaagtac ggcccgccgg cgtctttttc atcatggcga aatccgccgc
      301 cgggcaacga gcgatgcgcg atgatccact cggccgcgcg gactgcgtca gcgagcgcgg
      361 cggcgtctcc ggtgacgccg gagaatgtcg cgagggcgtt gatcgcccag ccgttttccc
      421 gcgcataaat gtgtcgatcg acgcgcggca cccctcgcgc gcggcgcgcc ccgtcacgca
      481 acgcaaaata atccgcggaa tgttcgccgg gcaccagatc cgcgtcctga ctggtataaa
      541 aggcgccctc cgggctggtc agaaagtcgc gcagatatcc acggatccga gtggcagctt
      601 cgagatactt cggatccttc cagagcgcgt aggcctcggc ataaagacgg aggttctccg
      661 cctggaactg catgatcttc tcgaaatgcg ggtgtttcca atcgccatcg gtcgagtatt
      721 gataaacgcc gccccagacc ggatcgatca actgcagttg cgcgtcgaga gtctcgcgcg
      781 ccatgcgctc gaactcgcgg tcgccggcca gcgtttgtcc gaggcaaaat tcgatgacat
      841 cccaatcaag gaacttttgc acagtgcccc agccgcgatt cttcttgtcg taatcatcgc
      901 gcagcacctg ctccaacttc tgcctcgctt ccggcgtcaa tccggcatcg ctgccgggta
      961 tgatgggcgg ctcgttcaca attgagggcc cgggcgaggg atcgtcgatg attgcctgga
     1021 gcatcgaagc catcagtttc ggcgggagat aaccctggcg ctttacgatc tcactgccgt
     1081 cgctgttgaa gaccaccgtc gctggccacc cgtagtcctc atagcgagcg gcaatatccg
     1141 gccgcgaatc ctgatcgacc ttcacggcga gataatgcga ctgaatcagc gcgacaactt
     1201 tcggatccgc gtaggtctgc gcgtccatca cgtggcacca atggcaccag actgcttcca
     1261 gatcgagcag gacgaaacga tgttcgcggc ttgcctgttc aaaaatcgaa tctgtccaag
     1321 gctgccaatt gatcttcggc tcttcggcaa gaagcggccg gaggacaacg agcgccagat
     1381 ttccgcagag aaccagccta acgaatcgcc ctcgcaggat catgccggct tgccctcgtg
     1441 gcgtttgaaa tatccgccga tcagcgcgtc cgccgagaaa gcaccggggc cgaaaacgaa
     1501 gaggatgacg acggcgaaga gaaagaggaa ttcgtcggcg gtgacgaatt tatccggatc
     1561 catgaagatg acgcgcacgc gatcgaaatc cgcggtgaga tacgcggtcg taagcagaat
     1621 catgagcgga accgaaatca gccgcgtggc caagcccacg agcaagagca ggccaccgaa
     1681 acactctgtg cacgcggcga gaatggcttg cgcgtgagga aaaggaatgc ccaagctctc
     1741 gaaatattgg gttggttttg acaagtccat cagcttgccc tggccgctga ggaaaaactg
     1801 gcaaccccag taaaggcgag tgaggaggag gagaggcgat cgcaacaggt tcgcgaacgc
     1861 gatcagcccg cggtaaaatt tttccgcgag tctcatcatt tcctgggccg gcaaaaccag
     1921 ccgagcgcag accagtcgtg gaaccactcc tgaatttgcc gggtccagtc cacgtcggac
     1981 cgggcggatt ctgaaagcgc ggcgaggcag gctttctcca cattcttccc gcgctgtaag
     2041 gcctgcagaa tcgcgaagcc ttccggctcg aggcgcttgt aatagagcat gttgtcgtag
     2101 cgatggacga cgagataaac acgctcgtgt ttcggcagtc gcatccggcg tttccgcacc
     2161 ggagccgccg gcatcgcgtc cggagtatta ctcgcttcgc cgcggagcga atcggcctcc
     2221 cgttttttca ccacgaggag aaatttatcg accgcataat tcagctcgag caacgaaaga
     2281 tagggctgca gaccaagccg taaacgcgcg ggcggtgtgt cgagaagatc atccgtcgtg
     2341 agctgcggcc gggcggcatc gtcgaaagcg acgacctgcg cccattcgaa tttcgccatg
     2401 tcgagggcga gctcctcatg cggcgcgatc cattgcggtt cctcccggag gaattgttcc
     2461 aggcgctgcc cgaggttgcg cagcgtaaaa gacgcggagg gatatttcgc cagatacgcc
     2521 gtggcgagtt tcatgaactt cttctcgccc acaatcgcgc gcaggccggg ataatcgtcg
     2581 tagagacaat cgagaacgcg gaaccaataa acgcgattgt aaatttccag gcgctcgaac
     2641 gaggtcagtc gatcgttagg cttgatgaac cgctgcgcga ccgcgcgcat gctgcggccg
     2701 tcggtccagc gcgtttgcat cccgtcgcgc gccgtcagcg ggcggaagat ggccgacgcc
     2761 atcagcgttt gcagctcttt caactgcggc cgcgacgcca cgggattgtt aggccgccgc
     2821 ggtttggaat tgtttttcga caaattgatt cgccttgagc gcctcgtgat gaacctcgtc
     2881 gaaggtcggg atgcggtcgt cccactcgag cagcgtggcg gttcgaccga cgagcttgat
     2941 cgcgtgggcg taaagtttcc aaacaggatc aagcaccgga tgatcgtgcg tatcgagaat
     3001 gtacttttcg aactttgagt gccccgcgac gtggatttgc ccgacgcgat gatgcgggac
     3061 gttgttcaga tattcgtagg gatcgaaatt gtgattccgc gacgaaacgt aaatgttgtt
     3121 cacgtcgagc agaattccgc agttggcgcg ttcgaccacc tcggaaagaa attcccactc
     3181 ggtcatctcc gaggcgtgaa attccgcgta gctgcttacg ttttcgacgc agatcggcac
     3241 ttcgagaaaa tcagccgcct cgcgaatgtt cgcggccgtg gcccgggcgg cggcaaaagt
     3301 gtatggcata ggcagaagat cgtgggtgta gcgaccgtcc acactgcccc agcaaaggtg
     3361 atcgcttagc catggcgtat tggtgcgttt caccaaccgc ttgatctttt tcaaatgctc
     3421 gcggctgagc cggtcggccg agcccaaata catcgccacg ccgtgctgga cgacgcggta
     3481 ttgctcgaga atctggtcga gcacctcgag cggccgaccg ccgtcgacca taaaattctc
     3541 cgagatgatc tcgaaccaat caaccaccgg cttcttttcc aaaatgtgcc ggtaatgcgg
     3601 cacgcgcagg ccaatgccaa tgccgtagtc gctgaaattg ttaaatgcat ttgcaggcat
     3661 gggatgagag ttgccaagag agggagaatc ggaggatcgg actccggctc tcgtatctcg
     3721 cgccaagacg cgcgaatgct tgcggtttag ttcgcggggc tggcggcagg atggctgccg
     3781 tcggtcgcac agccgccttt tcctttgcag gaattcttgc ctttgcagcc gttgtcgccg
     3841 gtcttgcagc cgcctttgcc tttacaggag ttctgtcctt tgcaggagtg aacgtctttt
     3901 ttcttggcca tctggctgac cgagacgcca gctttggtgg atacattggc ggcatgagtc
     3961 tgcaccgcga atgaaccgct cattaagccg gcgatcgcgg cgcccgctag gattgctttc
     4021 gatgtcatgt ttctctttct attttggttt cttacccggg gccggaggtt ccccgaaaca
     4081 ttcggggccg gtccggataa atgcatacgg gggaacgagg atagttgcgg ccaacaaggc
     4141 agacatacgg ggtatgagat cggagattgc ctggattatt cccgggacgc cagataattc
     4201 gtttcacgaa gcagattgga atttctgggg aataatacgc cggttggcaa gcggtggatt
     4261 catcccgtga gatgaattat ctttccggca ggatcccttt acgcacggcg gccagcggcg
     4321 cgccatcgtc gagggcgacg gttgcgccgc gccgttcgat cccgagttct ttccaaagtt
     4381 gatcgaggtt caccggatga ggcgaagccc tcatttcaga gtacagctcg cgtaataccg
     4441 gcacacccgt cgcgcggtcg ccggtctcga gcacgcgctc gatatcccaa tcggaagcga
     4501 tcgtcccgcc cgcctcgagg atgccgcgaa gcgcgtcttc gagacctttc gcattcgcgg
     4561 tgcgtcggcg aatttcgacg tcggcgagca agcagaaaag cgcaccgccc cagtaggtcc
     4621 ttccccaggt tggagttaaa tcgaggccgc ggtcgccggg ccccggcagc ccttgaggca
     4681 aatcgcgcac gaggtcagac cagacctgcc gcgcgcttaa ggtgccgatg cgcacccgtg
     4741 cgattggctc gacataggtc gccagccctt cctcgatcca atgattcttt tccggcacgc
     4801 tcggcaaggc gagatgcacc atttcatgcg tcatgagcca gtcggcgtcg aaatgggctt
     4861 tgctcgtgga acgaccgacc acaacggtga tcaagccacc atcgcttgtg ccaatggttc
     4921 tcccggaatc tatccggtca cctccgacca aacgaacccg gatgaccgcg tgggagacag
     4981 gatagcgtcc atagtatttt gtgaccgcgc gagccgcggt gttgatccat tcaagcgcga
     5041 gcgcgcgtgg gaaccccgga tcgtttccga ggaaaacgac gtcgattctg ccaccaccca
     5101 tttttatcgg accggtcgaa gcttcggaca ggaaaatccc accggagaga aaaagacaga
     5161 tcaggaacac gcgaccgaaa cagaagcctg aagacaccat ggttcatgcc ctattcaacc
     5221 ctggaacgcg aacggacaat ttccaagaac gccgccgatc gttagaccga gcggagtcga
     5281 agaataacgt gcctaacaaa tcgtgtgccc gccatcatga tgattcgtaa tcttcagact
     5341 tcgattgcgg tcgggcttgc gcttggtcgc taaccggacg cggttcgtcg cgccggcgtt
     5401 cccgcccgcg tccaccttcg ctgcgataat taccgcgcac ttcgccacca ccatcatctc
     5461 gccaattttc gcgacatttt ccgtgctggt tgaccccgaa atccgccagc tcggaaatgt
     5521 gaaccaagcc atccttgatc gacacgatgc ggccgtgata ggtctggcca cttcgatctc
     5581 cgtcatgccg gtgttcccgc gcgaccgcca ccgccgctgc cgcggcgctc accgcgatca
     5641 gctcgtccgc cgcccggctg ccgaaggcga ttttcgcatc gaccttgaag ggaaaaagca
     5701 ctgacgcgtg cgttttatac cgtcctggaa cgatccgtac tgggccagag ctgccatcgt
     5761 taggcgcggg gcggcgcgtt tccttggtgg aatggggcat gctattctag cgacaccgag
     5821 gctgcccgga ccgatctcag gacggcgcaa gccagcggcc aattatgcca gaatcgtcgt
     5881 ccagggattt gactctccag aggctggaac gcacctatgt tcggctggcg ttgctcgttc
     5941 ttttcggaat cgtcttgttt gtcggcctct gctgggccgg ctctcatgcc tacgtgcgct
     6001 ggcaggagca cagtttgatg cggcaggcgc acgcagcgct gcagcgaggt gacctgcgtt
     6061 gggcatcgct cgccgcccag cgggcttacg gtgtcaactc gtccagcctc gatgcctgtc
     6121 ggacgctggc agagatcgcc gaaaaagggc aggatgaggg agccatcgat tggcggcgcc
     6181 gggtcgtcga gatcgatccg cattcgctct ttaatcagct ggccctggca actaccgccc
     6241 tgcggttcgc caaaccggcg atcgccgcgg atgcgctcgc cgcagtgccc gccgagaagc
     6301 gcaacgacga cggctaccag gctgtcgccg cccgcgtctc gctcacaaaa aacgacctcg
     6361 ttgcgacccg cgtgcatctc gccgcagccg tccggctcgc cccgaacaat cccgatcacc
     6421 agctcgccct ggcacagttc gatctcgctt cgaaagacga tgtccaacgc gcggagggcc
     6481 gcaagctggc cgaattgctg aagaccaaac ccaccgtccg ccgcgaggcg ctggtgctcc
     6541 tgatcaacga cgccttgcgc cggcgatccc tcagcgaagc gaccaaattg acaaacgagc
     6601 ttgccgccct gcccaacgca tcgttcgcgg acgagatact caccgccagt tttcagcaca
     6661 cggtgaacga gcccaccttt attgccagcg tgacacggtt ggaagagcag gcagcgagtt
     6721 ccccggagaa ggcggcacaa ttgattgcct ggatgaacac cgaggcgctc agtctcctcg
     6781 cgatcgactg gagcaagaaa cttccggcga accttctcga taacctggcg gtgcgggttg
     6841 ctgtgggtga ttcctacgcc caatcgggca attggaaggg gcttgaagat attttgcagc
     6901 acggtacttg gacgggggcg gaatcgctca atctcgcctg gagggcgaaa gtggccgacg
     6961 agtcgagcga tgcggtgggg cgcgagaagt tttggaccga agcagtcgcc aaagcgcggg
     7021 gccaatccga gcagtcgaat ctcctcgcaa ggctcgccct ccaatggcac tggcccgaga
     7081 aggctaccgc cgttctctgg acttgctcgg agcagcgcga aacgcaactc cccgctttga
     7141 aggccctcta tctttactac acggaacagc gcgataccgc gggcacatac cgggtcctgg
     7201 gcaaactggt ggagctgttg ccggatgatc tttctatcag aaacaatttc atcctgatct
     7261 ctcttctgct tcagacagag ccgacgagaa tgcggaccat ggcgcgggaa ttgaatcaga
     7321 aggaaccagg caatggcagc ttcgcttcca cttacgcttt tgcgctctat cagactggcg
     7381 acacggcggg cgcacttcgc gtctttaaca ccctctcgcc ggagcaactg cgcgaccccg
     7441 cgattgcctt atattacgga gtgattctgg ccggagcggg gcgcaggcac gaagcggccg
     7501 cggcactcgc gaaaaccggc ggcgcgaaac tgctgccgga ggaaagggcg ctcgtggaaa
     7561 gcgtgcgggc caaactcgct cagcagtagg ttcttgactc ctcacgaaag cgcgggagca
     7621 aagggttggg tccccttgag gttgacgctc tggaggtggt caaatcagta gccggcgcgt
     7681 taaccggggc cgcataaact cgatgccaca ggacggacca ttaacgaaaa aagcggcgcc
     7741 ggaaacccgg cgccgcaccc aaaccagtcg aaaaaacctg gcggcctatt gtttggcccg
     7801 cactctgatg agacgcgcag tcaaggctt
//
LOCUS       DATMNS010000146         6406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2342571, whole genome shotgun sequence.
ACCESSION   DATMNS010000146 DATMNS010000000
VERSION     DATMNS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6406
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2342571"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VNW28_05135"
     CDS             complement(<1..464)
                     /locus_tag="VNW28_05135"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXA09343.1"
                     /translation="MKNGTTEPAEHALACARVAARFSERWLRLYAERKLRNDPVYLAA
                     FELFSASPSPLVDVGCGVGLLAFYLRERNFLPPVSGLDRDGRKIARARAAAANGVYRD
                     LEFIEQDVCAPIAPHGNVVLFDVLHYLSPNEQAHLLARLAPRVAPGGLLVIR"
     gene            502..1956
                     /locus_tag="VNW28_05140"
     CDS             502..1956
                     /locus_tag="VNW28_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HXA09344.1"
                     /translation="MPDLFSQYKVDDFFDEMFSAPGVARPHYSRILASFKEMGAADFE
                     RKRALAAASFLRQGVTFTVYNDDQGTERIFPFDLIPRIIPAREWDLVERGLVQRITAL
                     NLYLHDIYHGQNILRDGVIPKQIVWEATSFRPEFMNVDVPRNIYIHICGTDLIRDRAG
                     NYLVLEDNARCPSGVSYVLENRQAMKRIFPYLFAQCRVRPVEDYAQELLNVLRYIAPP
                     EAREPNVVLLTPGVYNSAYYEHSFLARSMGIEIVEGRDLLAQDGCVFMRTTKGRKPVD
                     VIYRRINDDFLDPRVFRKDSGLGVPGLVDAVRRGTVSLANSIGTGVADDKVIYHFVPK
                     MIRYYLGEDAILGNVPTYLAADPSDRAYILEHLPELVVKAANESGGYGMLIGSQATAA
                     EREKFAAQIRKDPRNYIAQPIVALSRSPSFCDDSIEGRHVDLRPYILSGEKVTIIPGG
                     LTRVALRAGSLVVNSSQGGGSKDTWVVEEEEGES"
     gene            2033..2998
                     /locus_tag="VNW28_05145"
     CDS             2033..2998
                     /locus_tag="VNW28_05145"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HXA09345.1"
                     /translation="MLSRVADSLYWMSRYIERAENNARILDVNLQLLLDLGNQSEAEI
                     MDHWAPVIASLEETELFGSLYEKADGSSVTEFVTLQKKNPNSIFSCLSHARENARTTR
                     EQISSEMWEQVNRLYLYVKSDGAKKLLRTSPHEFLKRIISGSHLFQGMTDATMSHGEG
                     WDFIRIGALLERADCTSRILDVKYHILLPSGEDVGGNVDTIEWMAVLKSCSGLEAYCK
                     LYVSQVAPWKVAQFLITDREFPRSIRFCIDSLDNALHRISGAPETRFTNEAERLSGRL
                     RSDVDYVTIGEIFEFGLHQYLEAIQERLVEVSEAMYDTYCASLGA"
     gene            complement(3129..3635)
                     /locus_tag="VNW28_05150"
     CDS             complement(3129..3635)
                     /locus_tag="VNW28_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09346.1"
                     /translation="MKPKRIKVNDRMQRGYEYLLTKPTGKDFAAGFRPELTPKQMLRL
                     GVFGGKYLTDCQAEFPDDWFDKAKLCSERHAPKLNFFGVNASQPLSVWREKGWIHHED
                     PRGWFQWYCRYYLGRRCVDDERQIKRWRAVRRHLAQLKKNCRRGDLTCRRKQRQALLH
                     WAYDSRKI"
     gene            complement(3723..5456)
                     /locus_tag="VNW28_05155"
     CDS             complement(3723..5456)
                     /locus_tag="VNW28_05155"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXA09347.1"
                     /translation="MAEGKLPSGVLRTIALLGASFTLLCALLTFVGWVSGLQLLASVR
                     ARYIPMAPSTALCFSLLGIGLLLQLQRPGRRWIPRLLAAAVLLLALAKVAEFLSGRHS
                     PIDIWFVRNPEMFGAVPTGRMAPLTAVDFIFTALGLFALTGGGFRQWAGALGAIGTII
                     SAVILVGYWYGTPLLYGGRIIPVALSTAWGFFLCGLAIVASAGAAEWPLRTFLGKSTR
                     AVLLRAFVPVIIAAALINGWVGATLLKTVRTNPAVVSALGALAFAFLITAIVSQVSRV
                     VGGRIDRAEAARNRARAELLKLNAQLEERVEKRTRELREKNLQMEEELQMARELQVAL
                     LPQKFPTIPAGAPPDESALGFLSLYFPTGDVSGDFFSVFPVGEKAAGVLICDVMGHGV
                     RSALVTGMIRGLVEEHSQLAHNPGELLTRINRALTVILKQAGTTMFATCFYVVADVQR
                     AELRFANAGHPSALHLRRDGASAEKLDGQEARGPAMGIFSSAHYSTSTRAMGKGDLVM
                     LFTDGLFEVEDATGAFFTEEELRTTVSRHAALAPEEFFNRVLADIRRYSQSETFADDV
                     CVVGMQIRHTE"
     gene            complement(5645..>6406)
                     /locus_tag="VNW28_05160"
     CDS             complement(5645..>6406)
                     /locus_tag="VNW28_05160"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HXA09348.1"
                     /translation="LGDLAANLAKIRNFAGRAKTAGAELVLFPEMADTGYTMQVIRER
                     ATPWSEGAVPELQKIARTLSLAIICGVSERVDDRIYNAQVVLDSSGAIVAKYRKTHLF
                     APIDEDKCCTPGDALVSFDSGPWKFGLTICYDLRFPEIYRALALRHRVSVFIISSAWP
                     FPRVEHQRVLATARAIENQSYVILANRVGRDDGVPFCGSSAVIDPSGVVVAAASADRE
                     ELLVADFSLEVIHAVRERMPVFAHRRPDLYGRDAG"
BASE COUNT         1291 a         1921 c         2025 g         1169 t
ORIGIN      
        1 tcgcggatga cgagcaggcc gcccggcgcg acgcgcggcg cgagccgggc gaggaggtgc
       61 gcttgttcgt taggcgaaag ataatgcagg acgtcgaaga ggacgacgtt accgtggggc
      121 gcgatcggtg cgcagacatc ctgttcgatg aattcgaggt cgcgataaac gccgttcgcc
      181 gccgccgcac gggcgcgcgc gatcttccgc ccgtcgcggt ccaggccgct gaccggcggc
      241 aggaaatttc gttcgcgcag ataaaaggcc agcaatccga cgccgcagcc gacatcgacg
      301 agcggcgatg gtgatgcgct gaacaactcg aaagcggcga ggtagacggg atcgttgcgc
      361 aatttccgct ccgcgtagag gcgcagccag cgttcggaga agcgcgccgc gacgcgcgcg
      421 caggcgagtg catgttccgc cggttcggtc gtcccgtttt tcattgcgtg cctctgcggc
      481 gctgacaaat taaatgtttc catgcccgac cttttctccc aatacaaggt cgacgatttt
      541 tttgacgaga tgttttccgc gcccggggtg gcgcggcccc attacagcag aatcctcgcg
      601 agtttcaagg aaatgggggc ggcggatttc gagcgcaaac gcgcgctggc cgcggccagt
      661 tttctccggc agggggtcac cttcacggtc tacaacgacg accaggggac ggaacggatt
      721 ttccccttcg acctgatccc gcgcatcatc ccggcgcgcg aatgggacct ggtggagcgc
      781 ggcctggtgc agcgaatcac cgcgctcaat ctttatctgc acgacattta tcacgggcaa
      841 aacattctgc gcgacggcgt gatcccgaag cagatcgtct gggaagctac ttcgttccgg
      901 ccggagttca tgaacgtcga tgtgccgcgc aatatctata tccacatctg cgggacggat
      961 ctgattcgcg atcgcgcggg aaattatctc gtgctcgaag acaatgcgcg ctgcccttcg
     1021 ggtgtttcct atgtgctcga aaatcggcag gcgatgaagc ggatctttcc ctatctcttt
     1081 gcgcaatgcc gggtgcggcc ggtggaggat tacgcgcagg agctgcttaa cgttctgcgc
     1141 tacatcgcgc cgccggaagc gcgcgaaccg aacgtcgtcc tcctcacgcc cggcgtttat
     1201 aactccgcct attacgaaca ttcgttcctc gcccgctcga tgggcatcga gattgtggaa
     1261 ggccgcgacc tcctcgcgca ggatggttgc gttttcatgc gcacgaccaa aggccgcaag
     1321 ccagtggatg tgatctaccg gcggatcaat gacgattttc tcgacccgcg cgtcttccgg
     1381 aaggactccg ggctgggcgt tccggggctg gtcgatgcgg tgaggcgcgg gaccgtgagc
     1441 ctggccaatt ccatcggcac cggcgtggct gacgacaagg tgatctatca tttcgtgccg
     1501 aagatgatcc gctattacct gggcgaggat gcgatcctgg gcaacgtccc gacttacctc
     1561 gctgccgatc cgtcggaccg cgcctacatt ctcgagcatc tgcccgaact ggtcgtgaaa
     1621 gcggcgaacg aaagcggcgg ctacggcatg ttgatcggtt cgcaggcgac cgcggcggag
     1681 cgggaaaagt tcgcggccca gatcaggaaa gatccgcgca actatatcgc gcagccgatc
     1741 gtcgccctct cccgttcgcc gtcgttctgc gacgattcga tcgagggccg ccacgtcgat
     1801 ttgcggccct acattctctc cggcgagaag gtgacgatca ttcccggtgg cctaacgaga
     1861 gtagccctgc gcgccggttc gctggtcgtc aattcctcgc aaggcggcgg gagcaaggat
     1921 acctgggtgg tggaggagga agagggggaa tcatgagaca agattgtagg gcgacccgcc
     1981 tgaggcgggt tgcccaggag tcagggcagg cggggcgccc gccctacaac ctatgctgag
     2041 ccgggtcgct gattcccttt actggatgag ccgctacatc gagcgggcgg aaaacaacgc
     2101 gcgcattctc gacgtcaacc tccagctcct gctcgatctc ggaaatcagt cggaggccga
     2161 aatcatggac cattgggcgc cggtcattgc ctcgctcgag gaaacggagc ttttcggttc
     2221 gctctatgaa aaggcagacg gcagttcggt gaccgagttt gtcaccttgc agaagaagaa
     2281 tccgaactcg attttctcct gcctctcgca cgcccgcgaa aatgcgcgca ccacccgcga
     2341 gcaaatttcg agcgagatgt gggagcaggt caaccggctc tatctttatg tgaaaagcga
     2401 cggggcaaag aagctcctgc ggaccagtcc gcacgagttt ctgaagcgga ttatctcggg
     2461 ctcgcatctt ttccagggca tgaccgacgc gaccatgtcg cacggtgagg gctgggactt
     2521 catccgcatc ggagcgctcc tcgaacgcgc cgattgcacg tcgcgcatcc tcgacgtgaa
     2581 atatcacatt ctgctgccct cgggcgaaga cgtcggcggc aatgtcgaca cgatcgagtg
     2641 gatggcggtg ctgaaatcgt gcagcggcct ggaggcctat tgcaaacttt acgtcagcca
     2701 ggtcgcgccc tggaaggtcg cgcagttcct catcaccgat cgtgaatttc cgcgctcgat
     2761 tcggttttgt atcgactcgc tcgacaacgc gctgcaccgg atttcgggcg cgccggagac
     2821 ccgctttacg aacgaagcgg aacggttatc gggccggctg cgttccgatg tcgattacgt
     2881 cacgatcggc gagatcttcg agtttggtct gcaccaatat ctggaagcga tccaagagcg
     2941 gctcgtggaa gtgagcgagg cgatgtatga cacctactgc gccagcctcg gggcgtagag
     3001 cggggctgtc cctcgagggt tggggtgcgt gcggctgccg gcgcagaatc gggccgcgcg
     3061 gaagcgcgtt cttccggagg gccatgcttc cgcatgtcca gttgcaggcc aaggcttagc
     3121 ctaacgactc atatttttcg cgagtcgtag gcccagtgga gcaatgcctg cctttgtttc
     3181 cggcgacagg tgagatcgcc gcggcggcaa ttctttttta gctgcgcgag atggcgacgc
     3241 acagcccgcc agcgcttgat ctggcgctcg tcatcgacgc aacgccttcc caggtagtaa
     3301 cggcaatacc actgaaacca gccgcgtgga tcttcgtgat ggatccatcc tttctcgcgc
     3361 cagacggaga gcggctggga ggcattgacg ccaaagaagt tgagcttcgg cgcatggcgc
     3421 tcggaacaaa gtttcgcctt gtcaaaccag tcgtcaggga attccgcctg gcaatcggtc
     3481 aggtatttcc cgccgaaaac acccaggcgc agcatttgtt tcggcgtcaa ctcgggacga
     3541 aagcccgcgg caaaatcttt cccggtcggt ttggtgagaa ggtattcata accgcgctgc
     3601 atccgatcgt tcaccttgat tcgcttcggt ttcacccgtg gcagtaaaag accggccgaa
     3661 agtgaaggca aaccagccgt gagcggcgcg gtgcctgtgg ctggacgcgg cggcggtccc
     3721 gcctattcgg tgtgtcggat ctgcattcca acgacgcaaa cgtcgtccgc gaaggtttcg
     3781 ctttgcgaat agcggcgaat atcagccagg acccggttga agaattcctc cggggccagc
     3841 gcggcatgac ggctgacggt cgtgcgcagt tcctcctccg taaaaaatgc gccggttgca
     3901 tcctcgacct cgaaaagtcc atcggtaaaa agcatgacaa ggtcgccctt gcccatcgct
     3961 cgggtggagg tcgaataatg ggcggacgag aatatgccca tggccggacc gcgcgcttcc
     4021 tggccgtcga gcttttcggc ggaggcgcca tcccgccgga ggtggagcgc gctgggatga
     4081 ccggcgttgg cgaagcgcaa ctccgcccgt tgcacatcgg ccacgacata aaaacaagtg
     4141 gcaaacatgg tcgtgcccgc ctgcttgaga atgacggtga gcgcgcggtt gatgcgggtg
     4201 agcaattcgc ccgggttgtg cgcgagctgc gaatgctcct cgaccaatcc gcggatcatc
     4261 ccggtgacga gcgcggaacg caccccgtgt cccatcacgt cgcagatcag gacgccggcc
     4321 gccttttcgc ccaccgggaa aacactgaag aaatcgccgc tcacatcgcc ggtcggaaag
     4381 taaaggctga gaaaacccag ggcgctctcg tcgggcggcg caccggccgg gatggtggga
     4441 aatttctgtg gcaacaaggc cacctgcagc tcgcgcgcca tttgcagttc ctcttccatt
     4501 tgcaggtttt tctcgcgcag ctcgcgggtc cttttctcga cgcgctcttc caattgcgca
     4561 ttgagcttga gcaactccgc gcgcgcccga ttgcgcgccg cttccgcgcg gtcgatccgc
     4621 ccgccgacca cgcgcgaaac ctgggacacg atcgcggtga tcaaaaaggc gaaagcgagc
     4681 gcgccgagcg cggaaacgac cgccggattc gtgcggacag tcttgaggag tgtggcgccg
     4741 acccagccat tgatgagcgc ggcggcgatg atgaccggca cgaaagcgcg gagtaacacc
     4801 gcccgcgtgg atttgccgag gaaagttcgc aagggccact ccgccgcgcc ggccgacgca
     4861 acgatcgcaa ggccgcagag gaaaaaaccc cacgccgtcg agagtgcgac cggaatgata
     4921 cggccgccgt agagcagcgg tgttccgtac caataaccga ccagaatcac tgcgctgatg
     4981 atcgttccga ttgccccgag cgcgcccgcc cattgacgga agccacctcc cgtcagcgcg
     5041 aacagaccga gcgcggtgaa aatgaaatcc accgccgtga ggggcgccat gcgcccggtc
     5101 ggcacggcgc cgaacatttc cggattccgg acgaaccaga tgtcgatcgg ggaatggcgg
     5161 ccgctcagga attccgccac cttcgcgagg gcaaggagaa gcaccgcggc cgccagcaac
     5221 cgcggaatcc aacgccggcc gggccgttgc aattgcagga ggagaccgat gccgaggagc
     5281 gaaaaacaga gagcggtgct cggcgccatc gggatgtagc gggcgcgcac gctggccaaa
     5341 agttgcagcc cggaaaccca gccgacgaaa gtcagtaacg cgcacaggag ggtgaacgac
     5401 gcgccaagca acgcgattgt gcgcaagaca ccgctcggga gtttgccctc ggccatgcgg
     5461 gaagtttgac cgaatcggac aaggctgtcc aaacctcttc gcggccggat aaccagtggc
     5521 gcgtatccgt catttgtatc gcaatgaact ttgtgtttga tatacgtgag tcacgtatat
     5581 gcacggcgac ggacaagacg cgaaaagaag gaacggctgc tatatgagca agtgatacag
     5641 ggcactagcc ggcgtcgcgc ccgtaaagat cgggccgccg atgagcaaaa accggcatcc
     5701 gttcccggac ggcatggatc acttcgagag aaaaatcagc cacgagcagc tcttcacgat
     5761 cagcggaagc cgccgccacg accacgccag aggggtcgat caccgccgag ctgccgcaga
     5821 agggcacgcc gtcatcccgg ccgacgcgat tcgcgagaat gacgtagctt tggttttcga
     5881 tcgcgcgcgc cgtcgccagg acgcgctgat gttcgacgcg cggaaacggc caggctgaag
     5941 aaatgatgaa gacgctgaca cggtggcgca gggcgagcgc gcgatagatt tccgggaaac
     6001 gaaggtcgta gcagatcgtc aggccaaatt tccacggccc agagtcgaag ctgaccagcg
     6061 cgtcgcccgg agtgcaacac ttgtcctcgt cgatcggcgc gaagagatgc gtcttgcgat
     6121 atttggccac gattgcgccg ctggaatcga gcacgacctg cgcgttgtag atgcgatcgt
     6181 cgactcgttc cgacactccg cagatgatcg cgagcgaaag ggtccgcgcg attttctgca
     6241 actcgggaac cgctccttcg ctccaaggcg tcgcccgctc gcggatgacc tgcatggtgt
     6301 agccggtatc ggccatttcg ggaaagagaa cgagttccgc gccggcggtc ttcgcgcgcc
     6361 cggcaaagtt gcggatcttc gcaaggttgg cggcgagatc gcccag
//
LOCUS       DATMNS010000147         6734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_553693, whole genome shotgun sequence.
ACCESSION   DATMNS010000147 DATMNS010000000
VERSION     DATMNS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6734
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553693"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1217)
                     /locus_tag="VNW28_05165"
     CDS             complement(<1..1217)
                     /locus_tag="VNW28_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HXA09349.1"
                     /translation="MGSPDRREFLRVLGGAALAGAWPSWLRAAENPDMVTISVFHTTD
                     LHGHILPTVDYNGQRDLGGLARCVTQIRRWRMENPNSLLIDIGDVYQGTEFALRDQGG
                     MMIELFNLLRYDAWIVGNHEFDWGVEPFRQAVARSAMPVLAANTLLEGKPAGHFADAQ
                     HPFAKIQPFVLKEIAGIKLAIIGLTTPGMPFWFPSKFIEGIEFQNPVEPVRRAIREAQ
                     AQGANAVVLAGHMGLKERTEGDDFANRCIALTAEFPQSAVFIAGHTHQDIPSRLTNGV
                     VLTQADHFGIHVGRVDLWFDRGSKKLLRQEARTELMDGRIPCDPVVLSRAQPQLDRAA
                     AVLAQPIGELADTLSVKSRVGEPSEVGWLIAGAIIEALADRDLAIDGVFHGLFDDQHA
                     FEKGPKTVGDIWE"
     gene            1279..1659
                     /locus_tag="VNW28_05170"
     CDS             1279..1659
                     /locus_tag="VNW28_05170"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXA09350.1"
                     /translation="MKLEGLDHIALAVRDVERSVRWYVEVLGLERQHEGAWDGVPKFV
                     GRGATGIALFPLRAPENSLGQGSPIRVLHFALRADRENFQRAQNELQAREVDFHFEDH
                     GIAHSIYFCDPDGHKLEITTYEVQ"
     gene            complement(1760..4936)
                     /gene="secA"
                     /locus_tag="VNW28_05175"
     CDS             complement(1760..4936)
                     /gene="secA"
                     /locus_tag="VNW28_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HXA09351.1"
                     /translation="MVSWILKKILGSKNQREIKRLQPIVRAINAFDEQFKALSDDELR
                     AKTATWNSELSTIEDPEALARRLEEILPEAFAVVKNAARRLTERQETFPVCDQPMTWN
                     MVHFDVQLIGGIVLHRGRIAEMATGEGKTLVATLPLYLNALAGRGAHLVTVNDYLARR
                     DAEWMGQLYTFLGLSCGCIQHDQPPDLRREQYAMDITYGTNSEFGFDYLRDNGMATTR
                     EQQVQRGYHYAIVDEVDSILIDEARTPLIISGPATVSTHQYDRLKPLVDQLVRKQNLL
                     CNRLAGEAVELFARFDHEKTPTFTPNEPAGQTISESVRKLATDLRAHLTEVAGTGKDG
                     QITDADVRYAASRLDPGVLMFKVKLGQPRNKQLLRMMEDPEKRKAIDKAELSFYTDTR
                     KEELFALKEELFFTMDEKSHEADLSEKGREFLNPDDPDSFVLPDLISEFTDIDLNRTL
                     SDEEKDRMKTERQEICDKQAEQIHNISQLLRAYCLFEKDVAYVVEENKVVIVDEYTGR
                     KMPGRRWSDGLHQAVEAKEGVQIDRETQTLATITIQNYFRLYHKLAGMTGTAETEANE
                     FHDIYKLDVAVIPTNRPVARADANDRIYKTRREKYNAVIKEIAERHAVSQPILVGTVS
                     VEASELLSRMLKREKIPHNVLNAKYHRQEAEIVQRAGQPGTVTISTNMAGRGTDIKLG
                     PGVPEAGGLYVIGTERHESRRIDRQLRGRCARQGDPGATRFYVSFEDDLMRNFGAADR
                     MTKIMERFGLEEGQELEHRWLNKSVETAQKRVEQRNYLARKRTLDFDDVMNNQREVVY
                     TYRNEVIDTEDPHKLVYEVIDEAVPAKVTEYLGTGASTDEPNHAGLLHWVNTTFPLGL
                     TKEKAQFETRSPDENAQFLIAKIKEAYERKSSHEEPAAVKSLERYIILNAIDRLWQEH
                     LYAMDALREGVYLRGYAQKDPLIEYKTEAYEMFVDLMANIKNEVLHNLFRSTSNLQAF
                     ETFLSTLPQFLMRQQAPNAPTANGPLPGRRPERIGGAMSGNGDAEGDGEVKLELAPVR
                     RETPKVGRNEPCPCGSGKKYKNCCGRIA"
     gene            5123..5545
                     /locus_tag="VNW28_05180"
     CDS             5123..5545
                     /locus_tag="VNW28_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXA09352.1"
                     /translation="MKKIVATNDAPAAIGPYSQAIRSGPLLFCAGQIPLDPQTGQIVS
                     DDISEQARRVLENIAAILKAAELNFSHVVKTTIFLTSMGDFQTVNEIYATYFRENPPA
                     RSTVAVSALPKGARVEIEVIAMASAAGDSGKTVGDTSG"
     gene            complement(5659..5871)
                     /locus_tag="VNW28_05185"
     CDS             complement(5659..5871)
                     /locus_tag="VNW28_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09353.1"
                     /translation="MHFSAAPIDDHTDASDDTAVPADDVDRFLHAAAAAHNILRDDEF
                     FVRRNHKSAAQDEAACFLLGEEVAFA"
BASE COUNT         1201 a         1974 c         2117 g         1442 t
ORIGIN      
        1 atctcccaga tatcgcccac cgtttttggc cctttctcga aagcgtgttg gtcgtcgaac
       61 aaaccatgga agacgccgtc gatggccagg tcgcgatcgg ccagcgcttc gatgattgcg
      121 ccggcgatca gccatccgac ttcgctcggc tcaccgactc gacttttcac actcagggtg
      181 tcagccagtt cgccgattgg ttgcgcgagg acggcggcgg cgcgatccag ctgtggttgg
      241 gcgcgactga ggaccaccgg atcgcacgga attcgaccgt ccatcagttc ggtgcgcgct
      301 tcctggcgga gaagtttttt cgacccgcga tcaaaccaca gatcgactcg cccgacgtgg
      361 ataccgaagt gatcggcctg ggtgagaacg acaccattcg ttaggcggct cgggatgtcc
      421 tggtgggtgt gcccagcaat aaaaacggcg gattgcggga actcggccgt gagcgcgatg
      481 caacggttgg caaaatcgtc gccctcggtt cgctctttca agcccatgtg tccggccagg
      541 acgacggcat tcgcgccctg cgcctgcgcc tcccgaatcg cgcggcggac gggctcgacc
      601 ggattctgga actcgatccc ctcgataaat ttcgagggaa accaaaaggg cattccaggt
      661 gtcgttaggc cgataatcgc aagcttgatc ccggcgattt ccttgagaac aaagggctga
      721 attttcgcga agggatgttg cgcgtcagcg aagtggccgg caggttttcc ttcgagcagt
      781 gtattcgccg cgaggaccgg catcgccgaa cgcgcgacgg cctgccggaa cggctccacg
      841 ccccagtcga actcgtggtt gcccacgatc caggcgtcgt agcgcaggag gttgaaaagc
      901 tcgatcatca tcccgccctg atcgcgcaac gcgaattcgg tgccctggta gacgtcgccg
      961 atgtcgatca ggagcgagtt tggattctcc atccgccagc gccgaatctg ggtcacgcaa
     1021 cgggccaggc cgccgagatc cctctgaccg ttgtaatcga ccgtcggcaa aatgtggcca
     1081 tgcagatcgg tcgtgtgaaa aaccgagatg gtgaccatat ccggattttc cgccgcgcgc
     1141 agccacgacg gccacgcgcc ggcgagcgcg gcgccgccga gaacgcggag aaattcacga
     1201 cgatcgggag aacccatttt cacggcgcac cgtttcgatt ttgactctcg cgggcggagc
     1261 gacaagcttt tccgcaagat gaagctggag ggtctggatc atatcgcgct ggccgtgcgc
     1321 gacgtggagc gctcggtgcg gtggtatgtc gaagtgctcg gcctggagag gcagcacgag
     1381 ggcgcgtggg atggcgttcc gaaattcgtg ggccggggag caaccgggat tgccttgttt
     1441 ccgctccgcg cacccgaaaa ttcgttaggc cagggtagtc cgatccgcgt gctgcatttc
     1501 gccctgcggg ctgaccggga gaattttcaa cgtgcccaga acgagttgca ggcgcgcgaa
     1561 gttgattttc acttcgagga ccacggcatc gcgcactcga tttatttttg cgatcccgac
     1621 ggccacaaac tcgagattac gacctacgag gtgcaataag aacgcgcggc gaggtgtttc
     1681 gttaggcgcg aggacgctgc ggaagcgcgt gcgccccgga gttcacgggc ggcggcgtgt
     1741 cgttaggcaa aggcgctcgt caggcgatgc ggccgcagca gtttttgtac tttttcccgc
     1801 tgccgcaggg gcacggctcg ttccggccga ctttcggcgt ctcgcggcgc accggagcga
     1861 gttccaattt cacttcaccg tcgccctccg catcgccgtt gccgctcatc gcgccgccga
     1921 ttcgctccgg ccgtcgcccg gggagcgggc cattcgcggt cggagcattc ggcgcctgct
     1981 ggcgcatgag aaattgcggg agggtggaaa gaaacgtctc gaaagcctgc aggtttgagg
     2041 tgctgcgaaa gagattgtgc agcacttcgt ttttgatgtt ggccatcagg tcgacaaaca
     2101 tctcgtaggc ttccgtcttg tattcgatga gcggatcctt ctgcgcgtag ccgcggaggt
     2161 agacaccttc acgaagcgcg tccatcgcgt aaagatgctc ctgccagagg cgatcgatcg
     2221 cgttcaggat gatgtaacgc tcgaggctct tgaccgcggc gggctcttca tggctcgact
     2281 tgcgttcgta ggcttccttg attttggcga tgaggaactg ggcgttctcg tccggcgagc
     2341 gggtctcgaa ctgcgccttc tctttcgtta ggccaagcgg gaaggtggtg ttgacccaat
     2401 ggagcaagcc ggcgtggttc ggctcgtccg tcgaggcgcc ggtgccgaga tattccgtca
     2461 ccttcgccgg aaccgcttca tcgatcactt cgtagaccag cttgtgcgga tcctccgtgt
     2521 cgatgacttc gttgcgatag gtgtaaacga cctcgcgctg gttgttcatc acgtcgtcga
     2581 aatcgagggt gcgtttacgc gcgagataat tgcgctgctc gacgcgcttt tgcgcggtct
     2641 cgaccgattt gttgagccag cggtgctcca gctcctggcc ttcctcgaga ccaaagcgtt
     2701 ccatgatttt tgtcattcga tcggccgcgc cgaagttgcg catgagatcg tcttcgaagc
     2761 tgacgtagaa acgggtcgct cccgggtcgc cctggcgcgc gcaacgtccg cgtagctggc
     2821 gatcgatgcg gcgcgattcg tggcgctcgg tgccgatgac gtagagcccg cccgcttccg
     2881 gcacgcccgg cccgagttta atatcggtgc cgcggccggc catgttggtt gagatggtga
     2941 cggtgcccgg ctggccggcg cgctgcacga tctcggcttc ctgccggtga tatttcgcgt
     3001 tcagcacgtt gtgcggaatt ttttcacgct tgagcatgcg gctgagcaac tcggacgcct
     3061 ccacgctcac ggtgccgacc aggatcggct gcgaaacggc gtgccgctcg gcgatttcct
     3121 tgattacggc gttgtatttt tcgcggcgcg ttttgtagat gcggtcgttg gcgtcggcgc
     3181 gcgcgaccgg gcgattggtc gggatgacag cgacgtcgag tttgtaaatg tcgtgaaatt
     3241 cgttcgcctc ggtctcggcg gtgccggtca tgccggcgag tttgtggtag agccggaaat
     3301 aattctggat cgtgatggtg gcgagagttt gcgtttcgcg atcgatctgc acgccttcct
     3361 tcgcctcgac ggcctggtgc aagccgtcgc tccagcgccg gcccggcatt ttgcggccgg
     3421 tgtattcgtc gacgatgacg actttgtttt cctccacgac gtaggcgacg tccttctcga
     3481 agaggcaata ggcgcggagc aattgcgaaa tgttgtgaat ctgctcggcc tgtttgtcgc
     3541 aaatctcctg gcgctcggtc ttcatccgat ctttttcctc gtcggagagc gtgcggttga
     3601 ggtcgatgtc ggtgaattcg ctgatcaaat ccgggagcac gaaagaatcg gggtcgtccg
     3661 gattcaaaaa ctcgcggccc ttttcggaga gatcggcttc gtgcgacttc tcatccatcg
     3721 taaagaagag ttcttccttg agggcgaaga gttcctcctt gcgggtgtcg gtataaaacg
     3781 agagttcggc cttatcgatc gccttgcgtt tttccgggtc ctccatcatc cgcaggagct
     3841 gcttattgcg cggctgtccg agttttacct tgaacatgag caccccggga tcgagacggg
     3901 acgcagcata acggacatcg gcatccgtga tttggccgtc tttgccggtg ccagcgacct
     3961 ccgtgagatg cgcgcgcaga tccgtggcaa gctttcggac cgactcggaa atcgtctgtc
     4021 ccgccggctc gttaggcgtg aaggtcggag tcttttcgtg atcaaaacgg gcgaatagtt
     4081 caaccgcctc gcccgcaaga cgattgcaga gtaagttctg cttgcgcacg agctggtcga
     4141 cgagcggctt gagccgatcg tattggtgcg tcgagacggt ggccgggccg ctaatgatga
     4201 gcggggtgcg cgcttcgtcg atcaggatgg aatcgacttc atcgacgatg gcgtaatgat
     4261 agccgcgctg cacctgctgc tcgcgggtcg tagccatgcc gttgtcgcgc aggtaatcga
     4321 aaccgaactc cgagttggtc ccgtaggtaa tgtccattgc gtactgctcg cgccggagat
     4381 cgggcggctg atcgtgctgg atgcagccgc aggataatcc gaggaaagtg tagagctggc
     4441 ccatccattc cgcgtcgcgc cgggcgaggt aatcgttcac cgtcacaaga tgcgcgcctc
     4501 gaccggcgag cgcgttgagg taaagcggga gcgtggcgac gagcgttttg ccttcgcccg
     4561 tggccatttc cgcaatgcga ccgcggtgca gcacgatgcc gccgatgagt tgcacgtcga
     4621 agtgcaccat gttccaggtc atcggctggt cgcagaccgg aaaggtttcc tgccgctcgg
     4681 tcaggcggcg ggcggcgttt ttcacgaccg cgaaagcttc cggcaaaatc tcctccaggc
     4741 ggcgcgccag cgcctcaggg tcctcgatcg tggaaagctc ggaattccag gtcgcggtct
     4801 tcgcgcgcaa ctcgtcgtcg gaaagcgcct taaactgctc gtcgaaggcg ttgatcgcgc
     4861 gcacgatggg ctgtaaccgc ttgatctcgc gctgattttt cgagccgagt attttcttta
     4921 gaatccaact aaccatgagg gcggtactat cgacgagatt cgcccacgat caatcgcggg
     4981 cggtctggtt gtagctgggg gcgttgaccc cggtgggggt aaagcgttgc cgcgcggccg
     5041 gggtcaacgc ccccggcttc aaaacgtccg gcgcgctcct cgtctccgtt gtgtgcggaa
     5101 attttgcggc taagctcggg cgatgaaaaa gattgttgca accaatgacg cgcccgccgc
     5161 gatcgggcct tattcgcagg ccatccgcag cggcccgctg ctcttttgtg ccgggcaaat
     5221 cccgctcgat ccccagaccg gccagatcgt ttccgacgat atttccgagc aggcgaggcg
     5281 ggtgctggag aatatcgccg ccatcttgaa agccgcggag ttgaatttca gccacgtggt
     5341 caagaccacc atcttcctca ccagcatggg tgatttccag accgtgaacg aaatctacgc
     5401 gacctatttc cgggaaaatc cgccggcgcg ttcgaccgtc gccgtttccg ccctgcccaa
     5461 aggcgccagg gtcgagatcg aagtcatcgc catggcgagt gccgccggcg acagcggcaa
     5521 gaccgtcggc gatacctcgg gctagtcggg gaggcgccgc gcgatgaatt gcggccagct
     5581 cgccgacaaa ctgcgcgatc ttgcctggga tagcgtgatc tcggcggcat cattgtcggc
     5641 taggagatcg gccggtcgtt aggcaaacgc cacctcttcg ccgagaagga agcaggcggc
     5701 ctcgtcctgc gccgcggatt tgtgatttcg cctaacgaaa aattcatcgt cgcggaggat
     5761 gttgtgagcc gcggcggccg cgtgcaggaa acgatcgaca tcgtccgccg gcacggcggt
     5821 atcgtcactg gcgtcggtgt gatcgtcgat cggagcggcg gaaaagtgca agattatggc
     5881 tgccccttcc ggagtttgat cgcgatgaac gtcgagacct tcccggcgga cgagcttcct
     5941 cccgatttgg ctgcaacccc cgcgtccaaa cccggcagtc gttgagccgt ttgccgcatt
     6001 gattcttcag gagggccggt ctatccgtga ctggccgggt tcattcccga gtccccatga
     6061 gtttgagttc gaacgccatt tgtattgcgg gcgcccaccg ctctggcact tccatgctga
     6121 cgcggctgct gcaccgctgc ggacttgatc tcgggccgga aagtgacctg atgcccgcag
     6181 catctgataa cccagacgag gcttttcggg acaagccggt ttggggttgg aaggatccgc
     6241 gcaattgcct cacaatgcgc ttttggacca gcatgctgcc aggcctcgag acagtcatca
     6301 tggcgcgcat tacgcgcgct cgctgcgcct ctaggaaatc ctggccatcc ggataagaat
     6361 gtcgaaaaat atctgacttg gcgggcgtct catcccgaaa ctgcgtccct gggcgacgag
     6421 atacaggctt tacggtgcta tggtattgcg ctgcccgccc ctttggtagg ccctgactcg
     6481 gtggcaactc ctcctcagcc cgctaccagc cctccgccgc acgagaatcc aatcgaatgc
     6541 gtcagggtgc gtcagaccgg agggcgctga agtctcgatc atctttcctg tctaaaacca
     6601 gatcgaattc acgaagacgt gtctcgcttc ggtgcagcgg cactccgatg gcatgcccta
     6661 tgaggtcatc gtagtggatg acggttcgac gccctcgacc gtcgcagtgc ccgccttgct
     6721 aaaaaacgcc aaag
//
LOCUS       DATMNS010000148         4500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2712166, whole genome shotgun sequence.
ACCESSION   DATMNS010000148 DATMNS010000000
VERSION     DATMNS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2712166"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VNW28_05190"
     CDS             <1..714
                     /locus_tag="VNW28_05190"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXA09354.1"
                     /translation="VPDLDAVIVPVGGGGLLGGVSLAIKTLRPQAKVIAVEADLVASF
                     SAALREGKPVRIETRPTLADGLAIAQVGSNAFEIARQHTDKAVLVNEEQIALAILRVV
                     ELEKSVLEGAAATPLAACLSGQLPELAGRRVVLLHCGGNIDPNVLSRVIERGLVADGR
                     LSRFEAVISDRPGGLADLAAQIAAAGASIKQVVHDRAFASSDVSAVNVLCTVETRDRA
                     HLEELLARLQKRGVKTAGS"
     gene            839..3778
                     /gene="gcvP"
                     /locus_tag="VNW28_05195"
     CDS             839..3778
                     /gene="gcvP"
                     /locus_tag="VNW28_05195"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HXA09355.1"
                     /translation="MDLVETEALEKTAAPEAQKSPAPRRGTAEELSPFVARHIGPNDE
                     EIAAMLAVLGFEDLDTFIAATVPPDIRAAQPLALPVGKSEQEALAELRKLSEQNKVAR
                     SFIGAGYSDCFVPPVIQRNILENPGWYTAYTPYQAEIAQGRLEALLNFQTMITDLTAL
                     DIANASLLDEATAAAEAMTLCHAVVGGRDTFFVSEMCHPQTIEVVQTRAEPLGIQIVI
                     GNPATFRFTEKVFGALLQYPTTDGAIIDYEPFIKGAHEAGALAIVAADILALTLLRPP
                     GEMGADVAVGTTQRFGVPLGCGGPHAAYFATRDQYKRHLPGRLVGVSHDAAGRPAYRL
                     ALQTREQHIRRDKATSNICTAQVLLAVMASMYAVYHGPEGLRRIARRVHESACRLADG
                     LEQLGYGIAHQDFFDTIRLDLGKRASGEFLRRAVQQNCNLRVLGPHSIGISLDETTTP
                     NDLEMLLSIFGENRAILPGFAPASRKSQIENRKSAFLTHPVFNTHDSETEMLRYLRRL
                     EARDLSLCHSMIPLGSCTMKLNASAEMFPVSWPEFARIHPFAPNEQTAGYRAICAQLE
                     RWLAEITGFAAVSLQPNAGSQGEYAGLLAIRGYHAARSEAHRNICLIPTSAHGTNPAS
                     AVMAGFRVVPVKCLEDGDIDLTDLRAKADAHRQDLAALMVTYPSTHGVFETTIREICQ
                     IVHEHGGQVYMDGANMNAQVGLCRPGDIGADVCHLNLHKTFCIPHGGGGPGVGPIGVA
                     PHLVDFLPGLPSLNPQTASGGSTLNSGPVSAAPFGSASILTISWMYIRMMGPAGLTKA
                     TKVAILNANYIAQRLDSYFPVLFKGKNGLVAHECILDLRPCKSAGIEVEDVAKRLMDY
                     GFHAPTVSWPVPGTVMIEPTESEPPHELDRFCEAMIAIRAEIDAIGAGKADPKNNVLK
                     NAPHTAEQVTSDRWDHPYSREQAAYPAPWTRAQKFWPAVARIDSVYGDRNLFCTCPPM
                     EEFTETR"
     gene            complement(3775..>4500)
                     /locus_tag="VNW28_05200"
     CDS             complement(3775..>4500)
                     /locus_tag="VNW28_05200"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HXA09356.1"
                     /translation="PAGVCLLEEWLPDGQLQAIAAENNLAETAFVVARGEHFDLRWMT
                     PKVEVDLCGHATLAAAHVLFHHRDFDGSALRFQTKSGDLIVERDGEKLVLDFPALPAE
                     PGGPTNELSAALGAAPQEVLKERDYIAVFGTQKEVAAIQPDFARIAALDVQGVVVSAP
                     GDDCDFVSRYFAPAAGIPEDPVTGSTHCTLIPYWSRRLGKRELFARQISSRGGELFLR
                     DRGARVSIAGAAVTYLEGQIYLA"
BASE COUNT          845 a         1565 c         1303 g          787 t
ORIGIN      
        1 gtccccgatc tcgatgcggt catcgttccg gtgggcgggg gcgggttgct cgggggcgtg
       61 tcgctggcga ttaaaacgtt gcggccgcag gccaaagtga tcgcggtcga agcggatctc
      121 gtcgcaagtt tttccgccgc cttgcgcgaa gggaaaccgg tccggatcga aacgcggccg
      181 acgctcgccg acggcctcgc cattgcccag gtcggaagca atgctttcga gatcgcccgc
      241 cagcacaccg ataaggccgt gctcgtgaac gaggaacaaa tcgcgctcgc catcttgcgg
      301 gtcgtcgagt tggaaaaaag cgttctcgaa ggcgccgccg cgactcccct cgccgcctgt
      361 ctctccggac agcttcccga gctcgccggc aggcgcgtcg tcctgttgca ttgcggcggg
      421 aacatcgatc ccaacgtcct cagtcgcgtg atcgagcgcg gactggtggc ggatgggcga
      481 ctctctcgtt tcgaagccgt gatcagcgac cggccgggcg gcctcgccga tctcgccgca
      541 caaatcgccg ccgccggcgc aagtatcaaa caagtcgtcc acgaccgcgc ctttgccagc
      601 tccgacgtct cagccgtgaa cgtcctttgc acggtcgaaa cgcgcgaccg cgctcacctc
      661 gaagagttgc tcgcgcgctt gcagaaacgt ggagtgaaaa ccgccgggag ctagcagtgt
      721 agcggcggtc tgggaccgcc gaagcaccgc ccgcacttta gcgcggttcg gcgctcacag
      781 agcgccgcta caatggtctg tcggcttgcg ctactttctc cctctcgtag ggtgcgccat
      841 ggatttggtt gagaccgagg cgctcgaaaa aacggctgct cccgaggcgc agaaatcacc
      901 cgcgccacgc cgtggaactg ccgaggaact gagccccttc gtcgcccgcc acatcggccc
      961 taacgacgaa gagatcgccg ccatgctcgc cgtcctcggc ttcgaggatc tcgacacttt
     1021 tatcgccgcc accgtcccgc cggacatccg cgccgcgcag ccgctcgctc tcccggtggg
     1081 caaatcggag caggaagccc tcgcggaact ccgaaagctt tcggagcaaa acaaggtcgc
     1141 gcgcagcttc attggcgccg gctattccga ctgcttcgtg ccgccggtca tccagcgcaa
     1201 catcctcgaa aatcccggct ggtacaccgc ctacacgccc taccaggccg agatcgcgca
     1261 aggccgcctc gaagcgctcc tcaacttcca gaccatgatc accgacctca ccgcgctcga
     1321 cattgcgaac gcctcgctgc tcgatgaagc gaccgccgcg gccgaggcga tgacgctttg
     1381 tcacgcggtc gtcggcggac gcgacacgtt tttcgtttcg gaaatgtgtc acccgcaaac
     1441 catcgaggtt gtgcaaaccc gggcggagcc gttaggcatt cagatcgtca tcggcaaccc
     1501 cgccacgttt cgctttaccg aaaaagtttt cggtgcgctc ctgcaatatc cgacgaccga
     1561 cggcgcgatc atcgattacg agccctttat caaaggcgcg cacgaagccg gcgcgctggc
     1621 catcgtcgcc gccgacattc tcgccctcac tctcctccgc ccgccgggcg agatgggcgc
     1681 ggatgtcgcc gtcggcacga cgcagcgttt cggggttccg ttaggctgcg gcggaccgca
     1741 cgccgcttat ttcgccaccc gcgaccagta caagcggcat ctgccgggac gcctggtggg
     1801 cgtttcgcac gatgctgcgg ggcggccggc ctaccgcctc gccctgcaaa cgcgcgaaca
     1861 gcacatccgg cgcgacaaag cgacgagcaa catctgcacg gcgcaggttc ttctcgcggt
     1921 catggcgtcg atgtatgccg tctatcacgg accggaaggt ctgcgccgca tcgcccgccg
     1981 cgtccacgaa tctgcctgcc gtctggccga tggtctcgag caactcggct acggaatcgc
     2041 acaccaggat ttcttcgaca cgattcgcct cgacctcggg aagagagcga gcggcgaatt
     2101 tctgcgccgt gcggtgcagc agaactgcaa cctgcgcgtc ctcggtccgc acagcatcgg
     2161 catctcgctc gatgaaacga cgacgcctaa cgaccttgaa atgcttctct ccatcttcgg
     2221 cgagaaccgc gccattctcc cgggttttgc cccggccagt cgcaaatcgc aaatcgaaaa
     2281 tcgaaaatcc gcgttcctca ctcatcccgt ctttaacacc cacgactccg aaaccgagat
     2341 gttgcgttac ctgcgccggc tcgaagcacg cgatctctcg ctctgccact cgatgattcc
     2401 gctcggctcg tgcacgatga agttaaacgc cagcgcggaa atgttcccgg tttcgtggcc
     2461 ggagtttgcc cgcatccatc ccttcgcgcc taacgaacaa accgcgggct atcgtgcaat
     2521 ctgcgcccag ctggagcgct ggctggcgga aatcaccggg ttcgccgcag tctcgctcca
     2581 gccgaacgcc gggtcgcagg gcgaatacgc cggcctgctc gccattcgcg gatatcatgc
     2641 tgcgcggagc gaggcccatc gcaacatctg cctgattccc acttccgccc acggcaccaa
     2701 tccggccagc gcggtcatgg ccggctttcg cgtggttccg gtaaagtgcc tcgaggacgg
     2761 cgacatcgac ctcaccgatc tccgcgccaa agccgatgcg caccgccagg atctcgccgc
     2821 cctgatggtg acttatccct ctacccatgg cgttttcgaa accacgatcc gcgaaatttg
     2881 ccagatcgtg cacgaacacg gcgggcaggt ttacatggac ggcgcgaaca tgaacgccca
     2941 agtggggctc tgccgcccgg gcgacatcgg cgctgatgtc tgccatctca acctccacaa
     3001 aacattctgc atcccgcacg gcggcggcgg ccctggtgtc ggcccgatcg gtgtggcgcc
     3061 gcacctggtc gatttcctcc ccggtctgcc ctctctcaac ccccaaaccg cctcaggcgg
     3121 atcaactctc aactccgggc cagtctccgc cgccccattc ggcagcgcga gcatcctcac
     3181 gatctcctgg atgtacatcc ggatgatggg ccccgctggc ctaacgaaag caaccaaggt
     3241 cgcaatcctg aacgccaact acatcgccca gcggctcgac tcctattttc ccgtcctctt
     3301 caagggcaag aacggtctcg tcgcccacga atgcatcctt gacctgcgtc cctgcaagag
     3361 cgccggaatc gaagtcgagg acgtcgccaa gcgcctgatg gattacggct ttcacgcccc
     3421 gaccgtttcc tggccggtcc ccggcacggt catgatcgag ccgacggaaa gcgaaccgcc
     3481 gcatgaactc gatcgtttct gcgaggcgat gatcgcgatc cgcgccgaaa tcgatgcgat
     3541 cggcgccgga aaagccgacc ccaaaaacaa cgtgctcaag aacgcgccgc acaccgccga
     3601 gcaggtgacg tccgaccgct gggaccatcc ctattcacgg gaacaagccg cttatccggc
     3661 accatggact cgcgcgcaga aattctggcc ggcggtcgcg cgcattgaca gcgtctacgg
     3721 cgaccgcaat ctcttttgca cctgcccgcc gatggaagag ttcaccgaga ctcgttaggc
     3781 aagataaatc tggccctcga ggtaggtgac cgcggcgccg gcaattgaca cgcgcgcgcc
     3841 gcgatcccgt aagaataatt cgccgccgcg ggacgagatt tgccgcgcaa aaagctcgcg
     3901 ctttcccaga cgccgcgacc agtagggaat cagggtgcaa tgcgtcgagc cggtcaccgg
     3961 atcctcgggg atgcccgcgg cgggggcgaa atagcgcgag acgaaatcgc agtcgtcacc
     4021 cggcgcggaa accaccactc cctggacgtc caaggcggcg atgcgcgcaa agtccggctg
     4081 gatcgccgcc acctcctttt gcgtcccaaa gacggcgatg taatcgcgtt ctttgaggac
     4141 ctcttgcggc gctgcaccga gcgcggcgga gagctcgttc gtcggcccgc ccggctccgc
     4201 cggcagcgcc ggaaaatcga gcaccaactt ttccccatcg cgttccacta tgaggtcgcc
     4261 gcttttcgtc tgaaaacgaa gcgcgcttcc gtcaaaatcg cggtggtgaa acagaacgtg
     4321 ggccgccgcg agggtggcat ggccgcaaag gtcaacttcc actttcggcg tcatccagcg
     4381 cagatcgaaa tgctctccgc gagcgacgac aaaggccgtt tccgccaggt tgttctccgc
     4441 cgcgatcgcc tggagttgac catccggcag ccattcctcc aggagacaga cgcccgccgg
//
LOCUS       DATMNS010000149         2342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3325272, whole genome shotgun sequence.
ACCESSION   DATMNS010000149 DATMNS010000000
VERSION     DATMNS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3325272"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /locus_tag="VNW28_05205"
     CDS             <1..2134
                     /locus_tag="VNW28_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXA09357.1"
                     /translation="IGRDDEIRRVMQILARRTKNNPVLIGEPGVGKTAVVEGLAQRIV
                     AGDVPEVLRPKRVVALDLAALVAGAKYRGEFEDRLKAVLKEITESEGQIILFIDELHT
                     LVGAGAAEGAMDASNMLKPALARGELRAIGATTLNEYRKYIEKDPALERRFQPVLIAE
                     PSVEDTIAILRGLKERYELHHGVRIKDAAILGAATLSHRYITDRFLPDKAIDLIDESG
                     ASLRMQLDSLPVEIDEIERRILQLEIERQALLRESDAHSKERLKQLESELAGLREESN
                     AKKAQWQVEKTAIAKIRGIKEQIEQLKAEEQRLERSGELAKVAEIRYGKLATAEREIA
                     AAQKNLEEVQKKGGMLKEEVDEEDIAKLVSKWTAIPVGRLLEGEAQKLVTMESRLRQR
                     VIGQDSALERVANSIRRNRAGLSDPHRPIGSFIFLGPTGVGKTELARALAEFLFDDEK
                     SMIRLDMSEYMEKHSVSRMIGAPPGYVGYDEGGQLTEQVRRHPYSVILFDEIEKAHPD
                     VFNILLQILEDGRLTDGKGRTVDFRNAVLVMTSNVGSSALFDLAGSDPERARKEAMEA
                     LRQAFRPEFINRIDDIVLFNPLGQEQLNKIIDLELVKVMRLLAERKVRIELTPAARER
                     LVRDGYDPAYGARPLRRTVQRLVQDPLAMRILDGSVLPGDLVRVDIDPNSDSMRFERV
                     EQKAEQEPAAKGEAAQPAASAAKSGRRR"
BASE COUNT          520 a          687 c          718 g          417 t
ORIGIN      
        1 catcggccgc gacgatgaaa tccgccgcgt catgcaaatt cttgcgcgcc gcacgaaaaa
       61 taatccggtg ctgatcggcg aaccgggcgt cggaaagacc gccgtcgtgg agggtctcgc
      121 acagcgcatc gtagcgggcg atgtgcccga agtgctgcgc ccgaagcgcg tcgttgccct
      181 ggatctcgcc gcattggtcg cgggggcgaa atatcgcggg gaattcgagg accgcttgaa
      241 agcggtgttg aaggaaatca cggaatccga gggacaaatc attctgttca tcgacgaatt
      301 gcataccctc gtgggcgcgg gcgcggccga aggcgcgatg gacgcttcca acatgctgaa
      361 gccggccctg gcgcgcggcg agttgcgcgc gatcggcgcg accacgctca acgaataccg
      421 caaatacatt gagaaagatc cggcgctcga acgccgtttc cagcccgtgc tgatcgccga
      481 gccatccgtc gaagacacca tcgcgattct gcgcggcttg aaagaacgct acgaactgca
      541 ccacggcgtg cgcatcaagg acgccgcgat tttaggcgcg gccacgcttt cacaccgata
      601 catcacagac cgttttcttc cggacaaagc gatcgacttg atcgatgaat ccggcgccag
      661 cctgcgcatg cagttggatt cgctgccggt ggaaattgac gaaatcgaac ggcgcatctt
      721 gcaacttgaa atcgagcgcc aggccttgct ccgcgaatcc gacgcgcatt ccaaagagcg
      781 tttgaaacaa ctggaatcgg agttggccgg tctacgtgag gagtccaacg ccaaaaaggc
      841 gcagtggcag gtggaaaaaa ccgccatcgc caaaatccgc ggcatcaagg aacagatcga
      901 gcaactcaag gcggaggagc agcgcctcga acgcagcgga gaactggcga aggtcgccga
      961 aatccgctac ggcaaactcg cgacggcgga gcgggaaatt gccgccgcgc agaaaaatct
     1021 cgaagaggtg caaaaaaaag gcggcatgct gaaggaagaa gtcgacgaag aggacattgc
     1081 caagctggtg agcaagtgga cagcgattcc cgtcgggcgc ctgctcgaag gcgaagcgca
     1141 gaaactcgtc accatggaat cgcgcctgcg ccagcgagtc atcgggcagg attcggcgct
     1201 cgaacgcgtc gccaattcca tccggcgaaa tcgcgccggg ctttccgatc cccaccggcc
     1261 gatcgggtcc ttcatttttc tcgggcccac gggtgtcgga aaaaccgaac tggctcgcgc
     1321 gctcgcggaa tttttgtttg acgacgaaaa gtccatgatc cggctggaca tgtccgagta
     1381 catggagaag cattccgtct cgcgcatgat cggcgcgcct ccgggctacg tcggctacga
     1441 tgagggaggt caactcaccg agcaggtgcg ccgccacccg tattccgtaa ttcttttcga
     1501 cgaaattgaa aaggcccatc ccgatgtgtt caacattctt ctgcagatcc tcgaggacgg
     1561 ccgcctcacc gacggcaagg gccgcacggt cgatttccgg aacgccgtgc tggtcatgac
     1621 gtcaaacgtc ggatcgagcg cgctcttcga tctggcgggg agcgacccgg aacgggctcg
     1681 caaggaagcc atggaagcct tgcgtcaagc gttccggccg gagttcatca accgcattga
     1741 cgatattgtg ctgttcaatc ccctcgggca ggaacagctc aacaagatca tcgatctcga
     1801 actcgtcaag gtgatgcgcc tgctcgcgga acgcaaagtg cggatcgagc tcacgccggc
     1861 ggctcgcgag aggctcgttc gagacggcta cgatccggcc tatggggcgc gtccgcttcg
     1921 ccgcacggtc caacggctcg tgcaggaccc gctggcgatg cgcatcctcg acggcagcgt
     1981 tttgccggga gatttggtcc gggtcgatat cgatccgaac tcggactcca tgcggtttga
     2041 gcgtgtggag cagaaggccg agcaagaacc cgctgcaaaa ggggaggccg cgcaacccgc
     2101 cgcctccgca gcgaaatccg ggcggaggcg gtaagcttca tttttcctga tgcgccagtt
     2161 tgacgtgctc ggccgaaact cctagactga ttttgcgtct atcaagatag gtggccatta
     2221 tgagagccgt aaagacgttg tttttattaa ccgcgttaac cctgctatta gtcctcggcg
     2281 gagatgccat cgccggtcag caagggatga ccttcggcct gatcctggcc gtggcaatga
     2341 ac
//
LOCUS       DATMNS010000150         8758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4406360, whole genome shotgun sequence.
ACCESSION   DATMNS010000150 DATMNS010000000
VERSION     DATMNS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8758
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4406360"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /locus_tag="VNW28_05210"
     CDS             complement(<1..1192)
                     /locus_tag="VNW28_05210"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HXA09358.1"
                     /translation="MKAAGKTLLSNSAWSLLNQGARVGTLAVVTIALSRHFGPQRFGA
                     LAFGLAFVRIFAVIAAFGLDRILVRQMTENPEEAGRMVHHAFRLKLVLALASYATLLC
                     LAFLIDPHARLTVAIVALAGAGLLCQPCDVFDLRFQSENRFRLSFFGRTIPIVASTGI
                     KLAALFAGAPLLVFAGLETVEALFVGLALWCLHRRQNPAPDFAKTPARPIEWRHLLGE
                     GMPLLLGSLAVMIYMRSDVLMLGKMAGFRAAGIYSAAAQVTEACALLPLAFAPALFPI
                     LVRWRKLGPRYYKQQFGRLFLGAALTGLAISLFLTLAARPIVLLLYGAQFLSAAPVLV
                     IHGWATIFVFLGIVQSGYDITEGLTWAATHRAALGAITNIGLNFILIPKYGASGAALA
                     TLVAQ"
     gene            complement(1189..2223)
                     /locus_tag="VNW28_05215"
     CDS             complement(1189..2223)
                     /locus_tag="VNW28_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09359.1"
                     /translation="MATVHVLTQYIWPDAAPTGLYAEQLAARIEQNGGRVTLVGGRGG
                     YRDLQRCRPLSEIVHLEHYRGCRGSLSQTFAEYAAVTSAFRNYIKRFVRQDDVVIVTS
                     APPNTVTLAKAIRRRGARCIYWLQDYYPELVRGLYEYPAPLRKAFRGFWDHQLAQWDR
                     VVKIGANLAGPAHNSIVIRNWPTMSFDEPNPPPEPRTAIYSGNLGYGHDVELLVEACG
                     KLRTVGYRVSMRSDGRGAWRLPAWLQPLPLENNPFKLREDLLRHEVHLVAANPKITQA
                     LFPSKIWNSLAAGRRLVCTGFAGEMAVELEASKTAPFQRHLEQWTHLINATQNFDQPG
                     RVASLEPALA"
     gene            complement(2235..3203)
                     /locus_tag="VNW28_05220"
     CDS             complement(2235..3203)
                     /locus_tag="VNW28_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXA09360.1"
                     /translation="MTTPSLTILIPCLNEAFGIANVVREYTAAFPQATVLVVDNGSDD
                     GTAGIARSAGAKVITERRRGKATAVATAFAAIDSELVLMVDGDGSYPADGARLLVEQY
                     LREPVDMITGIRSAQKATQAFRPLHQWGMSIFAAVLNSVFRFKPLDLFSGQRLFSRRF
                     YKHVPVLSRGFELEIELTIQAVDKGFAQTEIPVPFRSRAKGSASKLKTTRDGLRIFRL
                     LVVLFRDYRPLTFFGHIAAVAALLGLSAGCIPIAEYLTTGMVNRLPLAVLAASLVTLS
                     IIIGLVGLLLEANLRYHREAYHIQLRKYGAPAVQRTAGDFPRVIAG"
     gene            3303..4625
                     /locus_tag="VNW28_05225"
     CDS             3303..4625
                     /locus_tag="VNW28_05225"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HXA09361.1"
                     /translation="MLLLDQAVSTAAFAPAPAPVAPRHALRGRRATLLALVIILLAGC
                     FLRLPSFVFVDRHPTMHWLAPLHPTAKFQVLGFDEGIYRAYVSTLILHGLASYPAMAD
                     TYLQKQRSIQTAILPPTRFFYIGAAYTWHLIWGTGPRTSLNVVSSLCSMLLLVLSALF
                     AWRLGGAAMGLCVAALMAFAPTQIHMSQHALIDGVFAFWATLSLWLLWENMQRPNRWL
                     WLAAYAGALAVMVLTKENAMFAYIGILVLIAANHWLRFGRVTKGLCLVTVLGPLLGVV
                     ILVFLCGGLGTTIEIYRLFISKVSVLKYAIRTGDGPWYRYLVDLMVVSPAVLVLAIGG
                     IFRLKSSERAGLYLFLFVGASFLVMVNIRYGMNLRYTNMWDFPLRYLAVLCLWQIFPR
                     RNAQALLVGLCLAALCTFELRQYHLFFVENDLYELVPEDLLRAVKMLK"
     gene            complement(4627..6297)
                     /locus_tag="VNW28_05230"
     CDS             complement(4627..6297)
                     /locus_tag="VNW28_05230"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXA09362.1"
                     /translation="MQPVTRSADAGRWLVRVEWITVALLTLVAIALQIRFVTAVGGLW
                     RDETNSINLATLPTFADVWRFLDYDSFPVLYFAVLRAWSGIFGAGNDEALRVLGLLIG
                     LGILGALWANARAFGHRLPLLSLALVGLNPMIVRYGDSNRAYGLGILLILLTLRSFWR
                     LAERPSAPSAKEVTIAAVLAVLSVQCLYYNAVLLLAIAAGAAAVALRAKAWRTVGTII
                     GIGALAAASLLPYAPIIIRMRAWTFLVSYPATFSWLWKRAGEVMGSPEPAAIWLWVSL
                     VVIGLGTTLVALLLRRGSPARVPAAVLFGAIALVVGVAGYATFLRALNYYTQPWYYVT
                     LAAFAACTLDIILGASPMSGNHRVPAIILRCLRPSVALGLLCFAAPLTWYDLQTRHTN
                     VDLIASQFQKFSRKGDVIVIPRWECAISFCRYYHGQAEVLTIPPIADHRFHRYDLVLT
                     QMKTKDAERPVLDRMEKTLRTGHRVFLADALPFPAADAPLPTLRPPHKDQSGAWHGDP
                     YYAVWNAYAGRFLRAHATHAGRIEVPLPPGIKVQKFEELDPGVVAGWQ"
     gene            complement(6372..>8758)
                     /locus_tag="VNW28_05235"
     CDS             complement(6372..>8758)
                     /locus_tag="VNW28_05235"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXA09363.1"
                     /translation="NALIVGASGQDGRLLTRQLLDRGYAVRGWTRTKPAPQIECECAQ
                     IDLLDSLAIEKELRNLSPDEIYYLAAFHHSTEDSLALTPTELTQRSFEVQVAGLRHVL
                     EAIEAVAPRTRLFYAASSHVFGEAVTASQNEQTPFAPTSVYGISKAAGVACCRHFREK
                     GIFAATGILFNHESALRKTSFLSQKVAQGVLRARREPGFKLVLGDLEARVDWGYAPDY
                     VDAMFRILQLPKPDDFVIASGELHSVRQFAQAAFAAGGLDWKQHVETDARLLKRDSQP
                     LCGNAAKLRAATGWSPTVNFSEMVARLVHETEKTMPSPKLLIFIPTYNEAENAERLCR
                     ELSTLDLGADILFCDDGSPDGTGEIVDRLTQKFPHVQAMHREGKLGIGSAHRDGIRYA
                     YEHGYETLVTMDCDFTHQPADVQRLLAAARASGRAVAIGSRYMERDSLPGWNLMRRSL
                     TRFGHFLTTNLLRLPQDATGALRVYQLRLLPEELFERVRSRGYSFFFESLFVLHHNGF
                     AIEEMPIALPARTYGSSKMCLRETWRSARRLLKLYWASVRHPESFLLSSPVLPILGTE
                     APLQDPQGWDSYWTRGQEKSRRLYSVIASIYRRLAIRRSLNHFMRKHFAPGDRLLHAG
                     CGSGQVDTELSQEMKITAVDISLPALESYRRNNPHAEAIRHGDILHLDGVAGETFDGA
                     YNLGVVEHFTQEQIVQILREMGRTVKPGGKVVIFWPHRRATSVFVLKLIHRVLDLFRR
                     KGGEAVRLHPAEISLLESRAQAQAVVERAGFDLVEYYFGLRDLLVQAVVVGCKQ"
BASE COUNT         1752 a         2618 c         2766 g         1622 t
ORIGIN      
        1 cctgggcgac cagggtggcc agggcagcgc cgctcgcccc gtatttcggg atgagaatga
       61 agttcaaccc gatgttcgtg atcgcgccga gggcggcccg atgggtcgcc gcccaggtga
      121 gcccctcagt gatgtcgtaa ccgctttgca cgatgccgag aaaaacaaaa atcgtcgccc
      181 aaccatgaat gacgaggacc ggcgcggcag acaggaattg cgctccataa aggagcagga
      241 caattggccg ggcagcgagg gtcaggaaca atgagatggc gagcccggtg agggccgccc
      301 cgagaaagag gcgcccgaac tgttgcttgt agtagcgcgg ccccagcttg cgccagcgca
      361 cgagaattgg aaagagcgcc ggggcgaaag ccagcggcag gagagcgcac gcttcggtaa
      421 cctgcgccgc cgcgctgtag atgccggccg cccgaaagcc ggccattttc ccgagcatga
      481 ggacgtccga acgcatgtag atcataaccg cgagcgaacc aagaaggagg ggcatgccct
      541 cgcctaacaa atgccgccat tcgattggcc tggccggtgt tttcgcgaaa tccggcgcgg
      601 gattctgtcg ccgatgcagg caccaaagcg cgaggcccac gaacagcgcc tcgaccgttt
      661 ccaaacctgc aaagacgagc agcggcgctc ccgcgaagag ggcggccagt ttgattccgg
      721 tgctggccac gatcgggatg gtgcgaccga agaaactcaa tcgaaaccga ttctcggact
      781 gaaatcgaag atcaaagacg tcacacggct gacagagcaa accggccccg gccagcgcca
      841 cgatggcgac cgtcaggcgc gcgtgcggat caatcagaaa ggcgagacag agcagcgtgg
      901 cgtagctggc gagggcgagg accagcttga gacgaaaggc atgatgcacc attctcccgg
      961 cctcctccgg attttccgtc atttgcctaa cgagaatccg atcgagccca aacgctgcga
     1021 tgacggcgaa gatgcgcacg aaagcgaggc caaaggcgag cgcgccaaag cgctgcggac
     1081 cgaaatgccg gctgagcgcg atcgtgacca ccgccagcgt gccgactcgc gcaccctggt
     1141 tgaggaggga ccacgcgctg ttcgagagca gagtcttgcc ggccgctttc atgcgagcgc
     1201 gggctccagc gacgcgaccc gtccgggttg atcgaagttc tgcgtggcgt tgataaggtg
     1261 cgtccattgc tcgaggtgac gctgaaaggg cgcggtcttg gaggcttcga gctcgaccgc
     1321 catctcgccg gcaaatccgg tgcagacgag acggcgcccg gcggcgagac tgttccagat
     1381 ttttgaaggg aaaagcgcct gcgtgatttt tggattcgcc gcgaccagat gcacttcatg
     1441 gcggagcaga tcttcccgca gcttgaacgg gttgttttcc aggggcaggg gttgcagcca
     1501 ggccggcaac cgccaggcgc cgcgtccgtc ggagcgcatc gaaacccgat agccgacggt
     1561 gcgcagtttg ccgcaggcct cgaccaatag ctcgacgtcg tggccatagc cgaggttgcc
     1621 ggaatagatc gcggtgcgcg gttccggtgg cgggttcggc tcgtcgaaag acatcgtcgg
     1681 ccaattgcgg atgacaatgg agttgtgcgc cgggccggcg aggttcgcgc cgatttttac
     1741 cacccggtcc cactgcgcca gctgatgatc ccaaaacccg cggaaggctt tgcgcaaggg
     1801 cgccggatat tcgtacaatc ccctaacgag ttccggatag tagtcctgca accagtagat
     1861 gcagcgcgcg ccacggcggc ggatcgcttt cgccagcgtg acggtgttgg gcggcgcgct
     1921 ggtcacgatc acgacatcgt cctgcctaac gaatcgttta atgtagttcc gaaaggcgct
     1981 ggtgaccgcg gcatattcgg cgaaggtctg gctgagactg ccgcggcaac cgcggtagtg
     2041 ttcgagatgc acgatctcgg agagcggccg gcatcgttgc aagtcgcgat aaccgccgcg
     2101 cccgccgacc aaagtgacgc ggccgccgtt ttgctcgatc ctggcggcca gctgttctgc
     2161 gtagagaccc gtcggcgcgg cgtcgggcca gatatactgg gtaaggacgt gaaccgtcgc
     2221 catgcggtgc gacctcagcc agcgatgacg cgcggaaagt ccccggcggt ccgctgcacc
     2281 gcgggcgcgc cgtattttcg cagctgaatg tgataggcct cgcggtgata gcgcaggttc
     2341 gcttccagca gcaagccaac gagcccgatg atgatcgaaa gagtgacgag gctcgccgcg
     2401 agcacggcca acggcagacg attgaccata ccggtcgtca aatattcggc gatcggaatg
     2461 cagcccgcgg acaacccgag aagcgcggcg acggcggcga tgtgaccaaa aaaagtgagc
     2521 ggccggtaat cgcgaaagag caccacgagc agccgaaaaa tgcgcaagcc gtcgcgggtc
     2581 gttttgagtt tcgacgccga tcctttggcg cggctgcgga acggcaccgg aatctcggtt
     2641 tgggcgaagc ctttatcgac cgcctggatg gtcagctcga tttccaattc gaagccgcgg
     2701 gacagaaccg gcacgtgctt gtaaaagcgg cgcgagaaaa gtcgctgtcc ggagaaaagg
     2761 tcgagcggct tgaaacgaaa aacggaattg agcacagcgg cgaaaatgga catgccccac
     2821 tggtgcagtg gccggaaagc ctgcgtcgct ttttgcgcgc tccgaatgcc cgtgatcatg
     2881 tcgaccggtt cgcgcaggta ttgctcgacg agcaggcgcg cgccatccgc gggatagctg
     2941 ccatcgccgt cgaccatcag gaccagctcg ctatcgatcg cggcgaaggc ggtggcgacc
     3001 gcggtggcct tgccgcgccg gcgttcggta atcactttcg ccccggcgga gcgggcgata
     3061 ccggcggtgc cgtcgtcgga accgttgtcg accacgagga cggtcgcctg cgggaaagca
     3121 gccgtgtatt cgcgcacgac atttgcgatg ccgaaggcct cgtttaggca cggaatgagg
     3181 atggtaaggg agggcgtggt catcgaaggc gggcttgccc ctttttagga accatttttc
     3241 gtgccgcggg ttggaatgct tgctgctgtc gtgatggttg gcgcttcggc tccgcaacaa
     3301 tcatgcttct tctggatcaa gctgtttcga cggcggcctt cgcccccgcg cctgccccgg
     3361 tcgcgccccg tcatgctttg cgtggccggc gggcaacgct tcttgcgctc gtcattattt
     3421 tgctcgccgg atgtttcctg cgcctgccgt cattcgtctt tgtggaccgg catccaacga
     3481 tgcattggct ggcaccgctc catcccaccg ccaagtttca ggtgctcggt ttcgacgaag
     3541 gaatctatcg ggcctacgtc agcactctca ttctgcacgg cctggccagt tatccggcca
     3601 tggcggacac ttacctgcaa aaacagagga gcatccagac cgcgatcctg cctccgacgc
     3661 gatttttcta tatcggggca gcttatacct ggcacttgat ctggggcact ggtccgcgca
     3721 cctcgctcaa cgtcgtctcc agcctttgca gcatgttgct tttggtcctg agcgcccttt
     3781 tcgcctggcg actgggaggt gccgcgatgg ggctctgcgt cgccgcgctc atggccttcg
     3841 cgccgacgca aattcacatg tcgcaacatg ccttgatcga tggcgttttt gccttctggg
     3901 cgacgctttc tctctggtta ctctgggaaa atatgcaacg gcccaaccgc tggctgtggc
     3961 tcgcggccta tgccggtgcc ctcgcggtca tggtgctgac gaaagagaat gcgatgtttg
     4021 cctacatcgg aattctggtt ttgatcgcgg cgaatcactg gctgcggttt ggccgggtca
     4081 cgaaaggtct ttgcctcgtc acggtgctcg gacccctcct cggtgtcgtc atcctggtct
     4141 ttctctgcgg cggcctcggg acgacgattg agatttaccg tttgtttatc agcaaagtgt
     4201 cggtccttaa atacgcgatc aggaccggcg acgggccctg gtaccgttac ctggtcgacc
     4261 tgatggtcgt tagtccggcc gttctggtgc ttgcgatcgg cggaattttt cggctcaaaa
     4321 gctcggaacg ggccggcctg tatctgttcc tcttcgtcgg cgcgagtttc ctcgtgatgg
     4381 tgaatatccg ctatgggatg aacctgcgtt acaccaacat gtgggacttt ccgctgcgct
     4441 atctcgcggt cctttgtctc tggcaaatct tcccgcgaag aaacgcgcag gcgctcttgg
     4501 tcggtctttg cctcgccgct ctctgcacgt ttgagctgcg gcaataccac cttttcttcg
     4561 tcgagaacga cctttacgag ctggtcccgg aggacctgtt gcgcgcggtc aagatgttga
     4621 aataagctac tgccacccgg ccacgacgcc gggatcgagt tcctcaaact tctgcacttt
     4681 gatcccgggt ggaagcggaa cttcaatcct acccgcgtgc gtggcgtgag cgcgaagaaa
     4741 gcgccccgcg taggcgttcc aaaccgcgta atacggatcg ccgtgccagg ccccactctg
     4801 atccttgtgg ggcggacgca gagtgggcaa aggcgcgtcc gcggcgggga atggaagcgc
     4861 gtccgccagg aaaacccggt ggccggtccg gagcgttttc tccatccgat caaggacagg
     4921 tcgctctgcg tccttcgtct tcatctgagt gagcaccagg tcgtagcgat ggaagcgatg
     4981 atcagcgatc ggcggaatgg tgagcacttc tgcctggcca tgataatatc ggcaaaaaga
     5041 gatcgcgcat tcccagcgcg gaattacgat gacgtcccct ttcctggaaa atttttgaaa
     5101 ctgggaggcg atgagatcga cattggtgtg tcgcgtttgc aaatcgtacc aggtgagcgg
     5161 cgcggcgaag cagagcaacc cgagcgcgac ggacggtcgc aggcagcgga ggatgatcgc
     5221 cggtacccgg tgatttccac tcatcggcga tgcaccaaga atgatatcca aagtgcaggc
     5281 cgcaaaagcc gcgagagtca cgtaatacca gggttgcgtg tagtaattta gggcccggag
     5341 aaacgtggca tatccggcca cgcctacgac gagagcgatg gcgccaaaaa ggaccgccgc
     5401 cgggacccgc gcgggagagc cgcggcgcaa gagaagcgca acgagggtcg tgccaaggcc
     5461 gatcacaacg agactcaccc atagccagat ggcagccggt tcgggcgatc ccatcacttc
     5521 gcccgcgcgc ttccacaacc aagaaaaagt ggcgggataa ctcaccagaa atgtccatgc
     5581 ccgcatgcgg attatgatcg gcgcgtaagg caacaacgag gcggccgcca acgcaccgat
     5641 cccgataatc gtcccgaccg tgcgccatgc ctttgcccgc agggccacgg ccgccgcccc
     5701 agcggcgatc gcgagcagaa gaacggcatt ataatagaga cactgcacgc tcagcactgc
     5761 caggaccgcg gcgatcgtga cctcctttgc ggaaggagcc gagggtcgtt ccgcgagtcg
     5821 ccaaaaactc cggagcgtaa gcaggatcaa caaaatcccc agcccgtagg cgcgattcga
     5881 gtcgccgtag cgcacgatca ttgggttcaa gccgacgagg gcgagcgaga gcaaaggcag
     5941 gcggtggccg aacgcgcgcg cgttggccca taatgcccca aggatgccga gcccgataag
     6001 caaaccgagc acgcgcagcg cttcgtcgtt tcccgccccg aaaattcccg accatgcgcg
     6061 gaggacggca aaataaagaa ccgggaacga gtcgtagtcc aaaaagcgcc agacgtcggc
     6121 gaaggtagga agcgttgcca ggttgatgct gttggtctca tcgcgccaaa gacccccaac
     6181 agcggtgaca aatcgaatct gcagagcaat cgcgaccaac gtcaggagcg cgacagtgat
     6241 ccactctacg cggacaagcc acctccccgc atcggcggaa cgagtgaccg gctgcaaagc
     6301 gttgggcgat ttttcatccc catcacttga agccgcggct tgtcctgggc gtgaaagtaa
     6361 cgccggcccg ttcactgttt acagccgacg acgacggcct gaacgagcaa atcgcgcagc
     6421 ccgaaatagt attcgacgag atcaaatccg gcgcgttcga cgacggcctg cgcctgcgca
     6481 cgcgattcga gaagcgaaat ctccgccggg tgcaggcgca ccgcctcccc gcctttgcgg
     6541 cgaaagagat ccagcacccg atggattagc ttgagcacaa acacactggt cgcgcggcga
     6601 tgcggccaga agattacaac cttcccaccg ggcttcaccg tccggcccat ctcccgcagg
     6661 atttgcacga tctgttcctg tgtaaaatgc tcgactacgc caaggttgta ggcgccgtcg
     6721 aaggtttctc ccgccacgcc gtcgaggtgg aggatgtcgc catgtcgaat cgcttccgcg
     6781 tgcgggttgt tgcggcgata ggattcaagc gccggcagcg agatgtccac cgccgtgatc
     6841 ttcatctcct gcgagagttc tgtatccacc tgccccgatc cgcagccggc atgaagcagg
     6901 cgatcgcccg gcgcgaaatg tttgcgcatg aaatgattca ggcttcgccg aatggcgagc
     6961 cgccggtaga tgctcgcgat cacggaataa agacggcggc tcttctcctg gccgcgcgtc
     7021 cagtaactgt cccagccctg gggatcctgg agcggcgctt ccgtgccaag aatcgggagg
     7081 accggcgagg aaaggagaaa gctttcggga tgccggacgc tggcccagta aagtttgagc
     7141 aatcgtcgcg cgcttcgcca ggtttcgcgg agacacattt tcgagcttcc gtaggtccgc
     7201 gccggcaggg cgatcggcat ttcttcgatc gcgaaaccat tgtgatggag aacgaacaga
     7261 ctctcgaaga agaaagagta accgcgcgaa cgaactcgtt cgaacaactc ttccgggagc
     7321 aggcgaagtt ggtagacgcg caaagcaccg gtcgcatcct gcggcaagcg gagcagattg
     7381 gttgtgagga aatggccgaa gcgcgtgagc gaccggcgca tcagattcca gccgggcagg
     7441 ctgtcgcgtt ccatgtagcg tgaccctatc gcgaccgcgc ggccgctcgc tcgcgccgcc
     7501 gccagcaggc gctgcacatc cgcgggctga tgggtaaaat cacagtccat cgtgaccagc
     7561 gtctcgtacc cgtgctcgta ggcgtagcgg attccgtcgc ggtgcgcgct gccgattccg
     7621 agtttgccct cgcgatgcat cgcctggacg tgcggaaatt tctgcgtcaa ccggtcgacg
     7681 atctcgcctg ttccgtcggg cgacccgtcg tcgcagaaga gaatgtcggc tccgagatcg
     7741 agtgtgctta gctcgcggca gaggcgctcg gcattctcgg cctcgttgta ggtgggaatg
     7801 aagatcagca gtttgggaga tggcatggtt ttctctgttt cgtggacgag tctcgcgacc
     7861 atttcggaaa aattgaccgt gggtgaccag ccggtcgcgg cgcgcagttt tgcggcgttg
     7921 ccgcagagcg gttgcgaatc tctcttcagc aaacgcgcgt ccgtctcgac atgttgtttc
     7981 cagtcgagcc cgccggcggc gaaggctgct tgcgcaaact gccgcacgct atgtagctcg
     8041 cccgacgcga tcacgaagtc gtccggcttg ggcaactgga gaatgcggaa catcgcgtcc
     8101 acgtagtcag gcgcgtaacc ccaatcgaca cgcgcctcga gatcgccgag cacgagcttg
     8161 aaccccggtt cgcgccgcgc ccgcaggacg ccctgcgcaa ctttctgcga gaggaacgac
     8221 gttttgcgta gcgccgattc gtgattaaac agaatgccgg tcgcggcgaa gattcctttt
     8281 tcgcggaagt ggcggcaaca cgcgacgccc gccgccttcg agatgccgta aacggatgtc
     8341 ggcgcgaacg gcgtctgctc gttctgcgat gccgtgacgg cttcgccgaa aacgtgcgaa
     8401 gacgccgcat agaagagtcg tgttcggggc gccactgcct cgatcgcctc gagcacgtgg
     8461 cgcaggccgg cgacctgcac ctcgaaactg cgttgcgtca gctctgtcgg tgtgagcgcg
     8521 agagagtctt cggtggaatg atgaaacgcc gccagatagt agatttcatc gggtgacagg
     8581 ttccgaagtt ccttctcaat cgcgagagaa tcgaggaggt cgatttgtgc gcattcgcat
     8641 tctatctgcg gcgctggttt ggttcgggtc cagccgcgaa cggcgtagcc gcgatccaga
     8701 agttgccggg tcaacagacg gccatcctgt ccgctcgcgc cgacgatgag cgcgtttc
//
LOCUS       DATMNS010000151         6360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_215011, whole genome shotgun sequence.
ACCESSION   DATMNS010000151 DATMNS010000000
VERSION     DATMNS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215011"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="VNW28_05240"
     CDS             <1..312
                     /locus_tag="VNW28_05240"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXA09364.1"
                     /translation="LRSQKGTLEKSGLNVEVRIRHGDALDQIFAEVREGGHDLIVTGT
                     SQARGPFRHYIMGDLTRGILNRAECPILVARAGKIVDDEDAEGGFFHRLLHGFGAGKP
                     A"
     gene            390..917
                     /locus_tag="VNW28_05245"
     CDS             390..917
                     /locus_tag="VNW28_05245"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXA09365.1"
                     /translation="MNPKFSRFHNVRLAGLLAFVLGLVVFPGCSKKQPGQTAGSTGDY
                     VTGTPLPERQEGTSFFSGNVDRQQFQPIYFDFDTQTIRTSENGKIQQVASFLQQSSGQ
                     IIIAGFTDERGTPEYNRGLGERRAQSVREALIADGANAQKIQTVSFGAEMPADPASNE
                     SAWAKNRRAEIGVVK"
     gene            1039..1929
                     /locus_tag="VNW28_05250"
     CDS             1039..1929
                     /locus_tag="VNW28_05250"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXA09366.1"
                     /translation="MTAVRRNWIAHLWREWLFELRRPIAPAFARPHPEEWPNDRLTAA
                     WLGHSTVLLNFFGVTILTDPVLFPRIGLRLPFLTIGPKRLTRPALTFRELPPIDLVLL
                     SHAHFDHLDTRTLHQFGKRTQVVTASRTRDLLRWTRLRQITELRWGESHVAQTAEGGL
                     VVKAFRVQHWGARLQYDDYRGYNGYVMERHGRRVIFAGDTALTNSFAGLRNDRPCDLA
                     IMGIGAYEPWIRSHCTPEQAIAMANDAGARFILPIHHQTFRLSFEPFREPIERFTAAL
                     QNNPERIALREIGETFTLTA"
     gene            1979..2974
                     /locus_tag="VNW28_05255"
     CDS             1979..2974
                     /locus_tag="VNW28_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HXA09367.1"
                     /translation="MRAVVINSFGGIEGIDLQDVPTPPPPTADRIRIRVHAAGLNRAD
                     IMQREGKYPPPPGYPENIPGLEFAGEVEAVGAAVRAWQTGDRVFGITGGGAQAELVVV
                     AESNLARIPRELDWNQAAAMPEVFITAHDALFTRARLQMGERALIHAAGSGVGTAAVQ
                     LAHAAGATVYGTSRTAAKLAQARTLGLDESITVGESPQEFVERVQELTNGAGVEVILD
                     LVGGAYFPANLEVLAPRGRLICVGTTAGRKSKIDLGLVLRKRATIVGTVLRARSIEEK
                     AEATHRFSAHVLPLVSRGVVRPVVERVYPAAEVRAAHQHLESNRSCGKIVLDFSV"
     gene            3129..3842
                     /locus_tag="VNW28_05260"
     CDS             3129..3842
                     /locus_tag="VNW28_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HXA09368.1"
                     /translation="MAKFLNTSAANYYLEEIIRLAHDRLILISPLLKLNDRIKELLED
                     KNRLKIDIRIVCGKNELQPEEINWLRSLSFIRTSFCKNLHAKCYLNEEFCILTSLNLY
                     EFNQVNNNEMGILILRSEDPDLFAGASEEAQRIMRVSDEVRMTFEKVKRVTEDNAPSG
                     EEEHDGVGKLSTSRLAKKLGLKTQELTDHLVQLGAIEINDGHKHITPLGRRLGGELRV
                     SARFGPYFLWPEALPLAKA"
     gene            complement(3928..4146)
                     /locus_tag="VNW28_05265"
     CDS             complement(3928..4146)
                     /locus_tag="VNW28_05265"
                     /inference="COORDINATES: protein motif:HMM:NF020917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HXA09369.1"
                     /translation="MADVTIEIIKNGPYIVTGEVEIKDADGNSYPVEKRMALCRCGAS
                     SEKPFCDGTHSKIGFQAAEKAVPESAEG"
     gene            4308..4844
                     /locus_tag="VNW28_05270"
     CDS             4308..4844
                     /locus_tag="VNW28_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXA09370.1"
                     /translation="MPAKSEPPVIRLRDVEADDLGIFFEHQQDPVAVAMVAFHSRDRA
                     EFAQHWASLLADETILKKTIVVLSAVSADSPAQNQVAGHISSWASDGKREVGYWLDRA
                     FWGRGVATEALTAFLRLEQTRPLYASAAKHNAASIRVLQKCGFTISRSAHEAFDDVNA
                     SRACRVLKLSSKGDGCRA"
     gene            complement(4841..5932)
                     /locus_tag="VNW28_05275"
     CDS             complement(4841..5932)
                     /locus_tag="VNW28_05275"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HXA09371.1"
                     /translation="MECPFNHEQPLQYYGDYLAIDRLLALQKPLSLQEGKLLAHDEML
                     FIIVHQAYELWFKQILHEITACGEVLSKPAADDDGPDMNVVVHRLKRVVEIWKLLNHQ
                     VDVLETMTPLDFLEFRELLHGASGFQSKQFREIEASLGLKMENRFRPDYYKHTELGGF
                     NQQDFAEITEVEQKPSILGMVENWLGRMPFFEEEFWRDWEDPPDDSAAQPGRSPFWHR
                     YRLLYEQSLSERDNKPDLLGKFDDIFFKTGWGGFRPTALRAALFIMLYRDEPMFRLPF
                     ALLNSLIEIDEQLATFRYRHLQMVRRMIGTRVGTGGSSGEQYLQGAVNRNYIYKELAG
                     VITFLIERRHLPALPPRLHHALRFSFTNR"
BASE COUNT         1226 a         2000 c         1914 g         1220 t
ORIGIN      
        1 ctgcgcagcc aaaagggcac tctcgaaaaa agcgggctca acgtcgaggt gcgaatccgc
       61 cacggcgatg cgctcgacca gatcttcgcg gaagtacgcg aaggaggaca cgacctgatc
      121 gtgaccggca cctcacaggc ccgggggcct ttccgccatt acatcatggg tgatttgacc
      181 cgcggtattt tgaatcgcgc cgaatgtccg attcttgtcg cgcgggccgg aaagatcgtc
      241 gacgacgagg acgccgaggg cggatttttc catcggctcc tgcacggctt cggcgcgggc
      301 aaaccggcct aacgagtgga ctgcgtcccc gttggagcgg cgggcggctt gcatgccaag
      361 gtgctgccgc tatggtgcgc tcgccgctta tgaacccaaa attctcccgt tttcacaacg
      421 ttaggctggc cggcctgctc gccttcgtcc tcggtttggt agtcttcccc ggttgctcga
      481 aaaagcagcc cggccagacg gccggcagca cgggtgatta tgtaaccggc acgccgctcc
      541 ccgaacggca ggaaggcacg tcgtttttca gcggcaacgt cgatcggcag caattccaac
      601 cgatttattt cgacttcgac acccagacca tccgcacctc ggagaatggc aagattcagc
      661 aggtcgcgag cttcctccag cagagctccg gccagatcat tatcgccggc tttaccgacg
      721 aacgcggcac gccggagtac aatcgcggcc tgggcgaacg tcgcgcccag tcggtccgcg
      781 aagccctcat cgccgacggc gccaacgcgc agaaaatcca aaccgttagc ttcggcgcag
      841 aaatgccggc cgatcccgcc tccaatgaat ccgcctgggc gaagaatcgc cgcgccgaga
      901 tcggcgtcgt gaaatagctc caggattgcg gatgttgccc cctgggcaac acggggtcgg
      961 cggacggtcg cagaagaatt tttccgccga ccacgttcgt ccctgcgctt cgcaaacgcg
     1021 aagcgtctac agtgcgcaat gaccgccgtt cgccgcaact ggatcgcgca tctctggcgc
     1081 gaatggctct tcgagctgcg ccggccgatc gcgcccgcct tcgcgcgtcc gcacccggaa
     1141 gagtggccta acgatcgcct gactgccgcc tggctcggcc actccaccgt gctgctcaat
     1201 ttcttcggcg tcaccatcct gaccgatccc gttcttttcc cccgcatcgg tcttcgcctg
     1261 cctttcctga ccatcggccc gaaacgactc acgcgtccgg cgctgacttt ccgcgagctg
     1321 ccgccgatcg atctcgtctt gctttcccac gcgcatttcg atcacctcga tacccgcacg
     1381 cttcatcaat tcgggaagcg cacccaggtc gtcactgctt cccgtacccg cgatctttta
     1441 cgctggacgc gcctgcgcca gattaccgag ctccgctggg gcgaaagcca tgtcgcgcaa
     1501 accgccgagg gcggcctggt cgtgaaggcc tttcgcgtcc agcactgggg cgcgcgtttg
     1561 cagtatgacg actaccgcgg atacaacggc tacgtgatgg aacgccacgg acggcgcgtc
     1621 atcttcgccg gagacaccgc cctaacgaat agttttgccg gcctgcggaa tgatcggccc
     1681 tgcgatctcg cgatcatggg gatcggcgcg tatgagccct ggatccgctc ccattgcaca
     1741 cccgagcagg cgatcgcgat ggcgaacgat gccggggcgc gcttcatcct gccgatccat
     1801 catcaaacct ttaggctcag tttcgagccc ttccgcgaac cgattgaacg tttcactgcc
     1861 gcgttgcaaa acaatccgga gcggattgcc ctgcgcgaga tcggtgaaac gtttaccctc
     1921 accgcttagc gcccgcagtt cttcacccgc ccgaactcaa aggaacgaat ccaaaggtat
     1981 gcgtgcagtc gtcatcaatt cgtttggagg gatcgaggga atcgatttgc aggatgtccc
     2041 gacgcctccg ccaccgactg ccgaccgcat ccggattcgg gtccacgcgg ccggactcaa
     2101 tcgcgccgac atcatgcaac gcgagggaaa atatcccccg ccgcccggtt atcccgagaa
     2161 catccccggc ctcgaattcg ccggcgaagt ggaagcggtg ggcgcggcag tccgtgcgtg
     2221 gcagacgggc gatcgcgtct tcgggatcac gggcggcggc gcgcaagcag agctggtcgt
     2281 cgtggcggaa agcaatctgg cccgcatacc cagggaactc gattggaacc aggccgcggc
     2341 gatgccggag gttttcatca cggcgcacga cgcacttttc acccgtgcca ggctgcaaat
     2401 gggcgagcga gccctgattc acgccgccgg ttccggtgtc ggcacggccg cggtgcaact
     2461 cgcgcacgca gcgggcgcga cggtttacgg cacttcgcgc accgcggcaa agcttgcaca
     2521 ggcgcgcacc cttggtctcg acgagagcat taccgtgggc gagtcgccgc aggaattcgt
     2581 cgagcgcgtg caagaactga ccaacggcgc gggcgtagag gtcattctgg atctcgtcgg
     2641 cggcgcctat tttcccgcca atctggaggt gctcgctccg cgcggacgtc tcatttgcgt
     2701 cgggacgacg gcaggccgga aatcgaaaat cgatctcggc ctcgtcctcc ggaagcgagc
     2761 gaccattgtc ggcactgtgc tgcgggcccg ctccatagag gaaaaggcgg aggccacaca
     2821 tcgtttctcg gcccatgttt tgccgttggt ttctcgaggc gtcgtccggc cggtcgttga
     2881 gcgtgtttat ccggcggcgg aagtgcgcgc ggcgcaccag catctcgagt cgaatcgcag
     2941 ctgtggaaag atcgtcctgg atttttccgt gtagcagcgg gcgcctgctc gcgaaagccg
     3001 tcggattgtc gcccacaagg cttcggcgtc tcgcccgcaa cgctacgccg tcacacgtgc
     3061 tccctgccgc cgacacgtcg cgcctctaca ctcccgtcaa atggcatctt agctgcttta
     3121 tggtaattat ggcaaagttc ctaaatacca gcgcggccaa ctattatctc gaggagatta
     3181 ttcgcctcgc gcacgaccgg ctcatcctga ttagcccgct tttgaagttg aatgaccgca
     3241 tcaaagaact cctcgaggac aagaaccggc tgaagatcga tatccgtatc gtctgcggga
     3301 aaaacgagct ccagcccgag gagatcaact ggctgcggtc gctcagtttc atccgcacca
     3361 gtttctgcaa aaatctgcac gccaaatgtt atctcaacga ggaattctgc atcctgacga
     3421 gcctgaacct ctacgaattt aaccaggtaa ataacaacga aatggggatc ctgatcttac
     3481 gctcagaaga tccggacctc ttcgcgggcg cgtccgaaga agcccagcgg atcatgcggg
     3541 tgagcgacga agtgcggatg actttcgaaa aagtgaagcg cgtcactgag gacaacgccc
     3601 cgagcggcga agaggaacac gacggcgtcg gtaagctcag cacctcgcgg ctcgcgaaaa
     3661 agctcgggct gaagacccag gaactgaccg accacctcgt gcagctcggc gccatcgaga
     3721 tcaacgatgg ccataagcac atcacgccac tcggccgccg gctcggtggc gagttgcggg
     3781 tcagcgcgcg gtttgggccg tattttctct ggccggaagc cttgcccctg gcgaaggcct
     3841 aacggaggga cgtgcttccg cacgtccggg atcttccccc ggccagttcg cacacccgac
     3901 tggacgcgcg gaagcgcgtc gattcgttta accttccgcc gactctggga ccgctttctc
     3961 ggcggcctgg aaaccgatct tcgaatgcgt gccgtcgcaa aacggcttct cgctcgaagc
     4021 gccgcagcgg cagagtgcca tccgtttctc aaccggatag gaattcccat ccgcgtcctt
     4081 tatttccacc tcgcccgtca cgatgtaagg gccattcttg atgatttcga ttgttacgtc
     4141 agccatgcgc gcagcatcgc attcgcgagc gaaaaagaaa ccgccaccac tatcaggact
     4201 gtcgcaatct ctgtgaggaa acccgcgaag tctgggagcc gccattttgt ctctccggaa
     4261 gacggcgtcg ctcgctgaat tgcccgcccg gattaacttc tgacctcatg cctgccaaat
     4321 ccgaaccgcc ggtcatccga ttgcgcgatg tggaagcgga cgaccttggg atctttttcg
     4381 aacaccagca agatcccgtg gccgtggcga tggtcgcatt ccattcgcgc gatcgggccg
     4441 agttcgccca gcattgggcg tcgctgctgg ccgacgagac gattctgaaa aagacgattg
     4501 tcgtcctgtc cgctgtctca gcggattctc ccgcgcagaa tcaagtcgcc gggcacatca
     4561 gtagttgggc atcggacgga aagcgagaag tcggctactg gctcgaccgc gccttctggg
     4621 gccgcggcgt cgcgaccgaa gcgctcaccg ccttcctccg cctggaacag acccggccgc
     4681 tctacgccag tgcggcgaag cacaacgccg cctcaatccg agtcctgcaa aaatgcggat
     4741 tcacgatctc ccgttccgcg cacgaggcat tcgacgacgt caacgcttcg cgggcttgtc
     4801 gtgtgttgaa attgtcatct aaaggcgacg gctgtcgggc ctaacgattt gtaaaactga
     4861 agcgcaacgc atggtgcaag cgcggcggca aggcgggcag gtggcggcgc tctatcagga
     4921 aggtgatgac gccggccagc tctttgtaga tgtagttgcg attgacagcg ccctggaggt
     4981 attgctcgcc gctgctcccg cccgtgccga cccgggtgcc gatcatgcgc cggaccattt
     5041 gcaggtggcg atagcggaag gtggccagtt gttcgtcgat ctcgatgagc gagttgagca
     5101 gggcaaaggg caaccgaaac atcggctcgt cgcggtagag catgatgaag agcgcggcgc
     5161 gcagggcggt cggccggaag ccgccccagc cggtcttgaa aaagatgtcg tcgaacttgc
     5221 cgagcaggtc gggtttgttg tcgcgctcgg agaggctctg ctcgtaaaga aggcggtaac
     5281 gatgccagaa gggggaacgg cccggttgcg ccgccgagtc gtcaggcggg tcctcccagt
     5341 cgcgccagaa ttcctcctcg aagaagggca tccgcccgag ccagttctcg accatcccga
     5401 ggatgctggg cttttgttcg acctcggtga tctccgcgaa atcctgctgg ttgaagccgc
     5461 ccagctcggt gtgcttgtag tagtcggggc gaaaacggtt ttccattttc aggcctaacg
     5521 acgcctcgat ttcgcggaat tgtttgctct ggaacccgga ggcgccatgg agcagctcgc
     5581 gaaactcgag aaaatcgagc ggcgtcatcg tttccaggac gtcgacctgg tgattgagca
     5641 gtttccagat ctcgactacc cgcttgaggc gatgcacgac cacgttcatg tcggggccgt
     5701 cgtcgtccgc cgccggcttg ctcagcactt cgccgcaggc ggtgatctcg tgcaggatct
     5761 gtttaaacca gagctcgtaa gcctggtgga cgatgatgaa gagcatctca tcgtgcgcga
     5821 gcagcttgcc ctcctgcagg ctgagcggtt tttgcagggc gagaagccga tcgatcgcga
     5881 gatagtcgcc gtagtattgc aacggctgct cgtgattgaa ggggcactcc atcgcggcga
     5941 gtgtcgcgca tcgcgtgggg cggagtcaat gcagctccgg cggacggtat tgagcactgg
     6001 atcccgaagg agttagtcta gccgcggaat cgctcatcga tgcgattgag cttttcaccg
     6061 aataggggtt tgccggtaga cgcttcgctc tgcgcaacgc gggacgcgtt cacgctcgct
     6121 cgcagatcaa tcgaatggcg aacgcagacc cgtgtcacct gcaggacgac atttatagcg
     6181 cgcgcggaac ccactgcgct ggcctaacga cggcgccggc cgaaggagta gccgcgatgc
     6241 gtccgcccgc cgccgatggc acgtttgccg cccgcgcctg attccggctc gaagagcacg
     6301 gtgtaaatgt aggggaaact ctcgagcttc agtcgcggga ttttttgccc gataaagcgc
//
LOCUS       DATMNS010000152         3727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3688790, whole genome shotgun sequence.
ACCESSION   DATMNS010000152 DATMNS010000000
VERSION     DATMNS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3727
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3688790"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VNW28_05280"
     CDS             complement(<1..235)
                     /locus_tag="VNW28_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09372.1"
                     /translation="MGQVSGEESPNRSAIATAELPHHSFSYDALTVGIGVAIVGATAL
                     LYFLTAARDVVVGDTPELITAAVTLGIAHPPGYP"
     gene            complement(238..1137)
                     /locus_tag="VNW28_05285"
     CDS             complement(238..1137)
                     /locus_tag="VNW28_05285"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HXA09373.1"
                     /translation="MLQQFNEKNRDLVVNINGRLVHRDEAGVSPFDSAVQGGDAVWEG
                     LRLYNGRIFKLHEHLDRLYRSATALGFAEIPPNKKMIAEIKRTLAANEMRDGVHIRLT
                     LTRGVKITSGMDPRLNQSGPTLIILAEHKAPVYAKSGLTLITSTMRRPPPDVLDPRIH
                     HANLLNSILAKIEANNAGADDALMLDFAGHIAETNATHVFLVRKGEIATSRVVACPAG
                     ITRATVLEICTAEKIPQHEIYLTTEDAYQADEVFCTGTMGELAGVTRIDGNIIGNGEV
                     GPMTKRLSELYAQRTLAEGVKIF"
     gene            complement(1210..1941)
                     /locus_tag="VNW28_05290"
     CDS             complement(1210..1941)
                     /locus_tag="VNW28_05290"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09374.1"
                     /translation="MIQLLRIAMWSGPRNISTAMMRSWGNRTDTAVVDEPLYAFYLKA
                     TGKKHPVAEEVIAAGETDWREVTVRLTRDTPGSKRIYYQKQMTHHLLPEIERDWLADL
                     TNCFLIRDPAEVIASYIKKNDDPTLEDIGFVQQAEIFDWVRARSSAVPPVIDARDVLQ
                     NPERVLRLLCAAVAVEFDPAMLAWPPGLRATDGIWAKHWYGEVAKSTGFEPYRPRSAE
                     VPERLREIEARCRACYEQLYQHRLR"
     gene            complement(1959..2897)
                     /locus_tag="VNW28_05295"
     CDS             complement(1959..2897)
                     /locus_tag="VNW28_05295"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXA09375.1"
                     /translation="MSDPSSKVTRRQAACVLGGAAAATLLPWPMTAAEPTNEMLRRAI
                     PSSRETIPVVGLGTWQVFDVGDSPNERGPLRDVLARFVQLGGKVIDSSPMYGRAEAVV
                     GDLTSELKLREQVFLATKVWTSGKAAGQESMERSLQRLRTARLDLMQVHNLVDVDTQL
                     ATIRAWKEQGRIRYLGVTHYVDSAFPEVEKILRREKLDFLQINYSIIDRAADERLLPL
                     ARDRGVAVLINRPFASGDLFARVRSKPLPEWAAEFDCKSWAQFLLKWILANAAVTCAI
                     PATGNVRHLEDNMGAGVGRLPDEKMRQRMAQFVAGL"
     gene            3083..3565
                     /locus_tag="VNW28_05300"
     CDS             3083..3565
                     /locus_tag="VNW28_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXA09376.1"
                     /translation="MDSLPVTLRVNGKTYDLRIDPRTTLLDCLREHLHLTGSKKGCDH
                     GQCGACTVHINGRRVNSCLCFAVMQEGAEITTIEGIGQPEDLHPVQAAFLACDAYQCG
                     YCTSGQIMSAVALLREPCGADDAEVKEFMSGNICRCGAYPNIVAAIQTVRQDTTEKSG
                     "
BASE COUNT          701 a         1164 c         1134 g          728 t
ORIGIN      
        1 gcgggtagcc cggggggtga gcgatgccta gcgtcacggc ggcggtaatc aattcaggcg
       61 tgtcgccgac cacgacatct cgcgccgcgg tcagaaaata gagcaaggcc gtcgctccca
      121 cgatcgccac cccaatgccg acggtgagcg cgtcatagga aaaagaatgg tggggaagct
      181 ccgcggtggc gatcgcactt cgattcggac tttcctcccc ggagacctgc cccattccta
      241 aaatatcttc acgccttccg cgagggtgcg ctgcgcgtaa agctcgctca gtcgttttgt
      301 catcgggccc acttcgccat ttccaatgat gtttccgtcg atccgagtga cgccggcgag
      361 ttcgcccatc gtcccggtgc agaagacttc gtcggcctga taggcatcct cggtcgttag
      421 gtagatttcg tgctgcggga tcttttccgc ggtgcaaatc tcgagcaccg tcgcgcgggt
      481 gattccggcc gggcatgcca ccacccgact ggtcgcgatt tcgccctttc gcacgagaaa
      541 gacgtgggtc gcattcgtct ccgcgatatg gccggcgaag tcgagcatga gcgcgtcatc
      601 cgcgccggca ttgttcgcct cgattttcgc gaggatggag ttgagcaaat tggcgtggtg
      661 aatccgcgga tcgagcacgt cgggcggcgg ccggcgcatc gtgctcgtga tcagcgtcag
      721 gccgctcttc gcatagaccg gcgccttgtg ctcggcgagg atgatcaggg tcgggccgct
      781 ctggttgagg cgcgggtcca tcccggaagt gatcttcact ccgcgggtga gcgtgaggcg
      841 aatgtgcacg ccgtcgcgca tctcgttggc ggccagggtg cgcttgattt cggcgatcat
      901 ctttttgtta ggcgggatct cggcaaagcc gagcgcggtg gcggaacggt agagacgatc
      961 aaggtgttcg tgcagcttga agatacggcc gttgtagagg cgcaagcctt cccagactgc
     1021 atcgccgccc tgcaccgcgg agtcgaaagg actcacgccc gcctcgtcgc ggtgcacgag
     1081 ccggccattg atgtttacca ccaggtcgcg gttcttctcg ttaaattgtt gcagcatttc
     1141 ttgaccgcgg attacacaga tttcgcggat aactccaatc ttatcagcga aatctgtgta
     1201 atccgcgatt caacggaggc ggtgttgata gagctgttca tagcaggcgc ggcagcgcgc
     1261 ttcgatctca cgcagacgct ctggcacttc tgccgaccgc gggcgataag gctcgaaccc
     1321 agtcgatttt gccacttcgc cgtaccaatg tttcgcccaa attccgtcgg tcgcgcgcaa
     1381 acccggcggc caggcaagca tcgccggatc gaactcgacc gcgaccgctg cgcaaagaag
     1441 ccgcaggacg cgctccggat tttgcaggac atcccgcgcg tcgatcaccg gcggcaccgc
     1501 actcgaccgc gcccgcaccc aatcgaaaat ctcggcctgt tgcacgaacc caatatcctc
     1561 cagcgtcggg tcgtcgtttt ttttgatgta agaagcgatc acttccgccg gatcgcgaat
     1621 cagaaagcag ttcgtcaggt cagcgagcca gtcgcgctcg atttcgggca gaagatgatg
     1681 cgtcatctgc ttttggtagt agatccgctt gctccccggc gtgtctctcg ttaggcggac
     1741 ggtgacctcc ctccaatcgg tctccccggc cgcgatcacc tcctccgcca ccggatgctt
     1801 tttccccgtc gccttgagat agaacgcgta gagcggctca tcgactaccg ccgtatcggt
     1861 ccgattaccc cacgatcgca tcatcgcggt tgaaatattg cgcggcccgg accacatcgc
     1921 gatgcgcaga agctgtatca tgttaaccag gcgcttactt agaggccagc aacgaactgc
     1981 gccatgcgct ggcgcatctt ttcgtccggc aaacggccga caccggcgcc catgttgtcc
     2041 tccagatgcc tgacattccc cgtcgcagga atcgcgcagg tgaccgcggc atttgccagg
     2101 atccacttga gaagaaactg cgcccatgat ttgcaatcga actccgccgc ccactcgggc
     2161 aatggcttgc tccggacgcg agcaaagagg tcgccgctgg caaacggacg gttgatcagc
     2221 accgccaccc cccggtcacg cgccagcggg aggagccttt cgtccgcggc gcgatcgatg
     2281 atcgagtaat tgatctggag aaagtcgagt ttctcgcggc gcagaatttt ctccacctcc
     2341 gggaacgcgg aatcgacgta atgcgtgacc ccgagatagc ggatacgccc ttgctctttc
     2401 caggcgcgga tggtcgcgag ttgcgtgtcg acgtcgacca ggttgtggac ctgcatgagg
     2461 tcgagccgcg cggtgcgcag acgctggagg gagcgctcca tcgattcctg cccggcggct
     2521 ttcccgctcg tccaaacctt ggtcgcgaga aagacctgct cgcgcagctt gagctcgctc
     2581 gtcaggtcgc cgaccacggc ttcggcccgg ccgtacatcg gcgagctgtc gatcaccttg
     2641 ccaccaagct gcacgaagcg cgccagcacg tcccggagcg ggccgcgttc gttaggcgaa
     2701 tcgcccacgt cgaagacctg ccacgttcca agacccacga ccgggattgt ctcgcggctg
     2761 gaaggaatcg cgcgcctcaa catttcgtta gtcggttccg cggctgtcat cggccaggga
     2821 aggagcgtcg cggccgccgc gcccccgagc acgcacgccg cctgccggcg ggtgactttg
     2881 gaagatggat cggacactgg gaaaaacttg gcgcacgttt ccctgtcccg ctaatctgcg
     2941 tcgcccaaga agtggaaacg ttcacttttc gccaataatc gcttgtcccg attcgggatc
     3001 gtaacctacc gtgcgctcgg atcgcttttg tatgcctgat cgcgacgaga ctttcaaagg
     3061 gcaaacgcca tcgtcgaatt cgatggactc gcttccagtc acactgcggg tgaacggcaa
     3121 gacatacgac ctgcggatcg acccgcggac caccctgctc gattgcctgc gcgagcatct
     3181 gcatctcacc ggctcaaaaa aagggtgcga ccacggccag tgcggcgcct gcacggtgca
     3241 catcaacggc cggcgggtga attcctgtct ctgcttcgct gtcatgcagg agggcgcaga
     3301 gatcaccacg atcgagggca tcggtcaacc ggaggacctg cacccggtgc aggcggcctt
     3361 cctggcttgc gacgcctacc aatgtggcta ctgcacctcg ggacagatca tgtcggcagt
     3421 ggcgcttttg cgggagccgt gcggagcgga cgacgccgag gtgaaggaat tcatgagcgg
     3481 caacatttgt cgttgcggcg cttatccgaa cattgtcgca gcgattcaga ctgtgcggca
     3541 agacacgacg gagaaaagcg gatgaaagct ttcgcactca cgcgggccgg cgacagcgca
     3601 caggcgatcg cggcggcggc gcaagcgagc accgcgcaac agggcgcgga gattcgcttt
     3661 atcggcggcg gcacgaccct gatcgatctg atgaagttga acgtcgaaca gccgacgacg
     3721 ctgatcg
//
LOCUS       DATMNS010000153         4856 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4867645, whole genome shotgun sequence.
ACCESSION   DATMNS010000153 DATMNS010000000
VERSION     DATMNS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4856
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4867645"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /gene="fusA"
                     /locus_tag="VNW28_05305"
     CDS             <1..511
                     /gene="fusA"
                     /locus_tag="VNW28_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977811.1"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXA09377.1"
                     /translation="ENKVVGGNIPKEYIPACKKGIEEAMLNGVIGGYQVIDANVDIVD
                     GSYHDVDSNEMAFKLAAIFAVKDAFKKAKPILLEPIMKVENATPEEYQGDIMGDLNRR
                     RGKIASIEVKGHLTMVQAEVPLAEMFGYATAIRSLSKGRSSYSMEPSHFEQVPQQLVA
                     AVLDQKETK"
     gene            513..824
                     /gene="rpsJ"
                     /locus_tag="VNW28_05310"
     CDS             513..824
                     /gene="rpsJ"
                     /locus_tag="VNW28_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008933160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HXA09378.1"
                     /translation="MADAQRIRIRLKAFDHRMLDQSAIDIVETAKRTGARVAGPIPLP
                     TLIEKFTVNRSPHVDKKSMDQFEIRTHKRLLDIIEPTAKTVDELKKLNLPAGVDITIK
                     I"
     gene            860..1510
                     /gene="rplC"
                     /locus_tag="VNW28_05315"
     CDS             860..1510
                     /gene="rplC"
                     /locus_tag="VNW28_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HXA09379.1"
                     /translation="MSIGLLGKKIGMTSVYDAAGKLRPVTVIAAGDNTLLRRLTTEND
                     GYSAIQVGFDGQKESRVTKPLLGGFAKAGSEPKKLVREFRLAQDAPEGEINLAVTQFQ
                     AGDWVDVVGKSKGKGFQGVMKKHNFQGQGAAHGSKTHRRNGAIGNRSTPGRIWKNMGM
                     PGHMGDERVTVQNLQVLQVREADKIILISGAVPGANGSYVVVRPALKRAAATGASK"
     gene            1507..2121
                     /gene="rplD"
                     /locus_tag="VNW28_05320"
     CDS             1507..2121
                     /gene="rplD"
                     /locus_tag="VNW28_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HXA09380.1"
                     /translation="MSAKVLTVAAAKEAKINLIEGGRGTQAMHDAVVAMRAARRSGSA
                     NTKTKADVNLSGAKPWRQKGTGRARAGYKSSVIWRGGGVVFGPKPRDYTKKVSKTVRR
                     LAFQKALSERITAGDVLTVDAFSVKEPKTRAFVSLVHGHTDAKKVLLISDAFEETFYR
                     SARNVKPVLLATASDVNTEQLLAFDKIIVTGKALAQLAERTTRS"
     gene            2118..2402
                     /gene="rplW"
                     /locus_tag="VNW28_05325"
     CDS             2118..2402
                     /gene="rplW"
                     /locus_tag="VNW28_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HXA09381.1"
                     /translation="MNEPYDLIQTVQLTEKASLLSEKLNKYVFRVNPRANKIQIKRAI
                     ELLFKKQVVDVNTCNYAGKKKRERRADFGRKAHWKKAIVTLKEGEKIDLV"
     gene            2410..3264
                     /gene="rplB"
                     /locus_tag="VNW28_05330"
     CDS             2410..3264
                     /gene="rplB"
                     /locus_tag="VNW28_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXA09382.1"
                     /translation="MALRNFRPLTPTLRFTSLPMFQEVTKKQSKPVKSLTEPKKRSGG
                     RNNKGRLTSRHIGGGHKQKYRKVDFKRRKHGVAAEVTAIEYDPNRTARIALLKYSDGV
                     QSYILAPNGLQVGARVTAGAGSPPDVGNALPLSEIPLGANIHCIEIMPGRGGQIARSA
                     GQQATLSNREGGYALVKLPSGEIRRIHESAYATIGQVGNVDHMNVSSGKAGRTRWQGR
                     RPHVRGMAMNPIDHPMGGGQGKSKGGGGRHHPVSPWGQLAKGFKTRAKHKASDRFILE
                     RRKSKKKS"
     gene            3359..3637
                     /gene="rpsS"
                     /locus_tag="VNW28_05335"
     CDS             3359..3637
                     /gene="rpsS"
                     /locus_tag="VNW28_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXA09383.1"
                     /translation="MGRSLKKGPFVEPHLLEKIDKMNSSGVKKPVKTWSRRSMVTPDF
                     VGHTFLVHNGKIFQSVFVTENMVGHKLGEFSPTRIFKKHGSHTAKVTK"
     gene            3757..4479
                     /locus_tag="VNW28_05340"
     CDS             3757..4479
                     /locus_tag="VNW28_05340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09384.1"
                     /translation="MSDSNLETAEAKVTAETLLLQNETLRKQLSLDRETQKTLTDSLV
                     VSNAEAELFRRKYSELQLRMDALGVESVSKDRAKLEQRLLQAVSDLQLLEKEKDAYRE
                     QLLKMNETVFRYVKSAQQADPDARMEVETQLRTTNRLMAALGANAPDQSEGRGLMDGK
                     VISVREEWSLVVGNLGAQHGVKLGMPLRVVRAGKTIATLRVVDVREKISGAVVQELDS
                     EKVKISVGDRLQVDARQNTSLK"
     gene            4481..>4856
                     /gene="rplV"
                     /locus_tag="VNW28_05345"
     CDS             4481..>4856
                     /gene="rplV"
                     /locus_tag="VNW28_05345"
                     /inference="COORDINATES: protein motif:HMM:NF012462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXA09385.1"
                     /translation="MQVSSTYRYAKISPFKAREVTRAIQGLPVSAALDLVAFSPKKAA
                     ALISKTLKSAVANAENNANLRVDGLVVKEAIVGEGPTMKRMMTRARGSGSTIRKRSSH
                     IRIVLTDEIEIKTRAKKAKQAGG"
BASE COUNT         1150 a         1371 c         1517 g          818 t
ORIGIN      
        1 tgagaacaag gtcgtgggcg gcaacatccc gaaggaatac attccggcct gcaagaaggg
       61 catcgaggaa gcaatgctca atggcgtcat cggcggttac caggtgatcg acgccaacgt
      121 cgatattgtc gatggctcgt atcacgacgt tgattcgaac gaaatggcgt tcaagctggc
      181 ggcgattttc gccgtcaagg acgcgttcaa gaaggcgaag ccgatcctgc tcgagccgat
      241 catgaaggtc gaaaacgcga ccccggaaga atatcagggc gacatcatgg gcgacttgaa
      301 tcgccgtcgc ggcaaaatcg cgagcatcga agtgaagggc cacctcacga tggtgcaggc
      361 cgaagtgccg ctcgcggaaa tgttcggcta cgcgaccgcg atccgctcgc tttccaaagg
      421 ccgttcgagc tattcgatgg agccgtcgca tttcgaacag gtcccgcagc agctcgtcgc
      481 ggccgtgctc gaccagaagg agacgaaata atatggccga cgcgcagcgc attcgcattc
      541 gtttgaaggc attcgaccat cggatgctcg accaatccgc catcgatatc gtcgagacgg
      601 cgaagcgcac cggcgcccgg gtcgcaggac cgattccgct ccccactctg atcgagaaat
      661 tcacggtcaa ccgctcccca cacgtcgaca aaaagtcgat ggaccaattt gagattcgca
      721 cccacaagcg gctccttgat atcatcgagc cgacggccaa gacggtggac gagttgaaga
      781 aactcaacct gccagcgggc gtggatatca cgatcaaaat ttaacgtttc agcctcacca
      841 cttttgccag cgcacgacca tgagcatcgg acttttagga aaaaaaatcg gcatgaccag
      901 cgtctacgac gccgcgggca agctgcgtcc ggtgaccgtg attgcggccg gggataacac
      961 gctgctccgt cgcctgacga ccgagaacga tggctattcg gcgatccagg tgggattcga
     1021 tgggcagaag gagtcgcggg tcacaaagcc gttgctcggc ggattcgcga aggcaggctc
     1081 cgagccgaaa aagctcgtgc gggaattccg cctcgcgcag gacgcgccgg agggcgagat
     1141 caacctcgcg gtgacgcaat tccaggccgg ggattgggtc gatgtcgtgg ggaaatcgaa
     1201 aggcaaaggt ttccagggcg tgatgaagaa gcacaatttc cagggccagg gcgcggcgca
     1261 cggatcgaag acccaccgac gcaacggcgc gatcggcaac cgctccactc ccggccgcat
     1321 ctggaaaaac atgggcatgc cgggccacat gggtgacgag cgcgtgaccg tccagaatct
     1381 gcaggttctg caggtgcgtg aggcggacaa gattattttg atcagcggcg ccgttcccgg
     1441 cgcgaacggc agctacgttg tcgtccgccc cgccttgaag agagctgctg caaccggggc
     1501 cagtaaatga gcgcgaaagt cttaaccgtg gccgccgcga aagaggcgaa aatcaacttg
     1561 atcgaaggcg gtcgcggcac ccaggcgatg cacgacgcgg tcgtcgcaat gcgcgcggct
     1621 cgccgttccg gatcggccaa cacgaagacg aaagccgacg tcaatctttc cggcgccaag
     1681 ccgtggcggc aaaaaggcac cggacgggcg cgcgccggtt acaagtcgtc cgtcatctgg
     1741 cgtggtggtg gggtcgtctt tggtccgaag ccgcgcgatt acaccaagaa agtttcgaag
     1801 acggttcgcc ggctcgcttt tcaaaaagcg ctcagcgagc gcatcaccgc cggcgatgtc
     1861 ctgacggttg atgccttctc ggtcaaggaa ccgaagacca gggcgttcgt tagtctggtc
     1921 catggccaca ccgacgcgaa gaaggtgctg ctcatctcgg acgccttcga ggaaaccttt
     1981 tatcgttcgg cgcgcaatgt aaaaccggtc ttgctcgcga ccgcgagcga cgtgaacacc
     2041 gagcaactgc tcgccttcga caagatcatc gtgaccggca aggcgctcgc gcagctggcg
     2101 gagaggacca ctcgctcatg aacgaaccct acgatttaat ccagacggtg cagttgacgg
     2161 agaaggcttc gctcctgagc gaaaagctca acaagtatgt cttccgcgtg aacccgcgcg
     2221 cgaacaaaat ccagatcaag agagcgatcg agcttctctt taagaagcag gtcgtggacg
     2281 tgaacacctg caattatgcc ggcaagaaga agcgcgaacg gcgcgctgat tttggccgca
     2341 aggcgcactg gaaaaaagcc atcgtgaccc tgaaggaggg cgagaagatc gatctcgtct
     2401 agttgactta tggcactccg aaattttcgc ccgctcacgc cgacgctgcg cttcacgtcg
     2461 cttcccatgt tccaggaagt gaccaagaag cagtcgaaac cggtcaagag cctgaccgag
     2521 ccgaagaagc gcagcggtgg gcgcaacaac aagggccgtc tcacttcccg gcacatcggc
     2581 ggtggtcaca aacagaaata tcgcaaagtc gatttcaagc gccgcaaaca cggcgtcgcg
     2641 gccgaggtca cggcgatcga gtacgatccg aaccggacgg cgcgcatcgc gttgctcaaa
     2701 tacagcgacg gagtgcagag ctacattctc gctcccaacg gcctccaggt cggcgcgagg
     2761 gtgacggccg gagccggctc tccacccgac gtgggcaacg cgctcccgct cagcgagatt
     2821 ccgttaggcg cgaatattca ctgcatcgaa atcatgccgg gccgtggcgg ccagatcgcg
     2881 cgcagcgccg gtcagcaagc gacgctcagt aaccgtgaag gcggctatgc gctggtcaaa
     2941 ctcccttctg gcgaaatccg ccgtatccac gagagcgcct atgcgaccat cgggcaggtt
     3001 ggcaacgtcg atcacatgaa cgtctcgagc ggcaaagccg gccggacgcg ctggcagggc
     3061 cggcgtccgc atgtgcgcgg catggccatg aacccgatcg atcacccgat gggcggcggc
     3121 cagggcaagt cgaagggcgg cggcggccgg caccatccgg tcagcccgtg gggacaactg
     3181 gcgaaaggct ttaagacgcg cgcgaagcac aaagcgagcg atcgctttat tttggaaagg
     3241 agaaaatcga agaagaagag ctgaacgtga atcgtcagat cgtgaaccgt gaatcggcgg
     3301 gaaagaccgc cggtcgacga atctcgattc accaatctca aatctcgatt tcaaacacat
     3361 gggcagatcg ttaaaaaaag gaccctttgt cgagccgcat ctcctggaaa agatcgacaa
     3421 gatgaacagc tcgggcgtca agaaaccggt caaaacctgg tcgcgccgct cgatggtgac
     3481 cccggacttt gtcgggcaca cattccttgt tcataacggc aaaatctttc aatccgtgtt
     3541 cgtgaccgaa aatatggtcg gccacaaact cggcgagttc tcacctaccc gaatctttaa
     3601 gaagcacggg tcgcacaccg cgaaggtgac gaagtagttc aactgatggt tgcggattga
     3661 gacgatggga tgaaagtctc cgctctcact ccccggctcg cagtcgtgct ggtgtgtctg
     3721 tggtcagcct ccgggccggc cgcaggaaat agttctttgt cagattccaa tcttgaaacc
     3781 gcggaagcga aagtgacggc ggaaacgttg ctcttgcaga atgaaacctt gcgcaagcaa
     3841 ctttcgcttg accgcgaaac gcagaagacg ctgaccgaca gtctcgtcgt atcgaacgcg
     3901 gaagcggaat tgtttcgccg gaaatattcg gagctgcaat tgcggatgga tgcgctcggg
     3961 gtcgagtcgg tcagtaaaga ccgggcaaaa ctcgagcagc gtctattgca agcggtcagc
     4021 gacctgcaac tcctggagaa ggagaaggac gcttatcgcg agcagttgct gaaaatgaac
     4081 gagacggtct tccgctatgt gaagagcgcg cagcaggccg acccggacgc gcgcatggaa
     4141 gtggagacgc agttgcgcac gacgaaccgg ctcatggccg ccctgggcgc gaatgcgcct
     4201 gaccagagcg aggggcgcgg cctaatggac ggcaaggtga tcagcgtgcg ggaagagtgg
     4261 tcgttagtcg tggggaacct cggcgcgcaa catggagtga aactcgggat gccgctgcgg
     4321 gtggtgcggg ccgggaagac gatcgcgacc ttgcgggtgg tggacgtgcg cgagaagatc
     4381 agcggcgcgg tcgttcagga attggattcg gagaaggtaa aaataagcgt aggcgatcgc
     4441 ctccaggtgg atgcgcgcca aaatactagt ttaaagtaaa atgcaagtta gctcgacata
     4501 tcgttatgcg aagatttcgc cgttcaaagc gcgcgaggtc actcgcgcca ttcagggcct
     4561 gccggtttcg gccgcgctcg atctcgtcgc gttcagcccg aagaaggcgg cggcgttgat
     4621 cagcaagacc ctgaagagcg cggtcgccaa tgcggaaaac aacgccaacc tgcgggtcga
     4681 tggactcgtg gtgaaggaag cgatcgtggg cgagggcccg acgatgaagc gcatgatgac
     4741 gcgcgcccgc ggcagcggca gcacgatccg caaacggagc agccatattc ggatcgtctt
     4801 gaccgacgaa atcgagatca agacgcgcgc gaagaaagcg aagcaggccg gcggcg
//
LOCUS       DATMNS010000154        13858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2723773, whole genome shotgun sequence.
ACCESSION   DATMNS010000154 DATMNS010000000
VERSION     DATMNS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 13858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13858
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2723773"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..736
                     /locus_tag="VNW28_05350"
     CDS             398..736
                     /locus_tag="VNW28_05350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXA09386.1"
                     /translation="MKKIEAIIKPFKMEDVKEALAEIGVEGMTVSEVKGFGRQKGHTE
                     IYRGSEYTVDFLPKVKLEIVLTDELAQRAVGVITLAAKTGKIGDGKVFVSDVEQTIRI
                     RTDERGADAV"
     gene            769..1635
                     /locus_tag="VNW28_05355"
     CDS             769..1635
                     /locus_tag="VNW28_05355"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXA09387.1"
                     /translation="MNDYLLSLLLGVIEGLTEFLPVSSTAHLRISEALFRIDLGNGFW
                     KMYSIVIQLGAILALAAFFWRRILNFLRTFPDGERGDRTLFTHPLSLVMIAFVVTAIP
                     AFLLTKLIGKNLENLHVMAWALLIGGIVMWLVDSLFVRPSVHHMEEANLPTAIWIGAV
                     QVLSAVFPGTSRSMCTIAAGQVAGMSREAALEFSFFLSIPTMFVATGYDLLKTLKPHP
                     GTEALAPLAMTPHLWIVLGLGFLTSFVVALMVVAWFLHWVRRHGFTPFAIYRIVLGLG
                     LLAALSRGMLGH"
     gene            1662..2063
                     /locus_tag="VNW28_05360"
     CDS             1662..2063
                     /locus_tag="VNW28_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09388.1"
                     /translation="MQTETVLPDLQSCVLCEDVRCEFNGMQTLVGVLNIVPTPKLPVN
                     YLRLCIWTRWCSGAGRFHQRSKIIGPDDTQTVAQAEVDFDLKEMEGHATNVHYFGGVQ
                     FQQFGLHHVEIYLGDELRLRFPLPVIEVKQA"
     gene            complement(2133..2285)
                     /locus_tag="VNW28_05365"
     CDS             complement(2133..2285)
                     /locus_tag="VNW28_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HXA09389.1"
                     /translation="MRTILLIILILLLIGAFPAWPYSTGWGYYPTGGVGLVLLIVLIL
                     VLVGRI"
     gene            2472..3740
                     /locus_tag="VNW28_05370"
     CDS             2472..3740
                     /locus_tag="VNW28_05370"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXA09390.1"
                     /translation="MANTILVGAQWGDEGKGKIIDVLTGKADIVVRSQGGNNAGHTVI
                     HGAKKFVLHLIPSGILRRGKICVIGNGVVIDPVALVSEIDGLRKAGIKIGKNLLISDC
                     AHLVLPYHRALDEQRELRKGHARIGTTKRGIGPAYGDKAARTGLRIADLIQPALFSQK
                     LRAKMRENNSILKALGARPISFAKVNAEFLAAGERLRPFVANTVVYLHKALLQGKEIL
                     FEGAQGTFLDIDHGTYPYVTSSNTTAGGACTGTGVAPHRMDRVVGVMKAYTTRVGEGA
                     LPTEDATIAGMLHEMGREFGATTGRARRCGWFDAVATRYATMINGIDDLAITNLDGLD
                     SVDPIKVCVGYRLDGKKLNVPPSDAAQWTRCRPVYLQMPGWKRSTERAKNFSDLPNEA
                     QNYVRRIAELTGARLTMVSVGPARGQTLHL"
     gene            3745..4458
                     /locus_tag="VNW28_05375"
     CDS             3745..4458
                     /locus_tag="VNW28_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HXA09391.1"
                     /translation="MAAPHKAIPRHVAIIMDGNGRWAKERGLPRIKGHEEGAKAVKAC
                     VEGCGELKIEFLTLYAFSAENWRRPKSEVGALMRLLEQFLKEKTSEMAENNVRLQAIG
                     RLTDLPKACQKQLHKSIEQTAKNKGLTLILALSYGGRLEIIDGIKSLLRAIDLGHIDR
                     AMIDVEMFSKHLYTRYYPDPDLLIRTSGEMRISNFLLWQLSYTEMYVTPKLWPDFDKE
                     DLFAAVEEFGRRQRRYGAL"
     gene            complement(4540..5700)
                     /gene="hemW"
                     /locus_tag="VNW28_05380"
     CDS             complement(4540..5700)
                     /gene="hemW"
                     /locus_tag="VNW28_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HXA09392.1"
                     /translation="MHIPFCARICPYCAFYKERADPAQAARFCDAVLAELTVAASLRE
                     THEMARELNENGGTLARAAHRAAATTTLRPETIFFGGGTPTALTTAQLDALFRGFQVQ
                     LNLSELREWTFEANPGSVSERKAGLLRDHGVNRISLGVQSFDDGLLQLLGREHSAAQA
                     EASFDLLRRSGFKNLNIDLMFGLPGQTLAQWEATLQRAIALGPDHVSAYCLTYEEDTD
                     FFRRHARGEFRTDPEADADFFTRATDLLGKAGYEQYEVSNYARPGFQSVHNRAYWTGH
                     DYLGIGPSAVSTVGLRRWQNISDYRAYADRVLAGETASTGDEILTPQMKRSEAVALSL
                     RTSRGVPAEWVDDRPNEVEEFIRLGLMQARAQRYVLTPAGRFLADSVAEAFL"
     gene            5735..6844
                     /locus_tag="VNW28_05385"
     CDS             5735..6844
                     /locus_tag="VNW28_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HXA09393.1"
                     /translation="MSIAFPPIKSTIAARLIRSIAFLFAGVGWCAAAFAQGPPPPAPP
                     PPGPTPSIVYRASNPAAITDYRTNPEVVRRMTDELVLAVTHQPDIASAWRSLVKPNDR
                     VGIKISAAGGELFTTHRDIVNAIVDGLVAAGHQRQDIIVWDKSLGGIKEGGYTRGEGY
                     QLKSIPPRDGYDPKAIISEPFLGNLIWGDFEYVGNKGQVPLLSDTANTSSLSHFSKIV
                     ANEVTKIINLPVLSDSVRNGVAGCLYNVTIPNIDNWRRFGVPPDFGASSIPEIYNDPH
                     IGQKVVLNLMDGLLAQYAGGPQSQPGYAFPFATLYASKDPVAIDSIALRQLEEWRKKG
                     KLPAIKRMGAHVLMADQLGLGNANPVQIQIRDVGR"
     gene            6841..7791
                     /locus_tag="VNW28_05390"
     CDS             6841..7791
                     /locus_tag="VNW28_05390"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HXA09394.1"
                     /translation="MKAILRSVSRSFYLTIRFLPRPLREPVSLAYLLARATDTIADTA
                     AIPAATRLTTLLAFARVIAGESDFDAVAEPLRAFAAQQSDPAEHKLIENLRGCIDWLA
                     TVNRADRDDIRNVLQTIVRGQELDVRRFGDPAVVTSLRSAAELDEYTFLVAGCVGDFW
                     TKLGFRHLRSFASRSPNEMAKLGIEYGKGLQLINVLRDSGADAKCGRGYLPSDELAGQ
                     PVAEVFAHWLDQAEERLEAGIDYCSALTNWRMRFATALPALIGARTLTLLRQAGPEAR
                     ARKIKVARKTVRRILVASLAAAASPTALRALFQRLLEAPR"
     gene            complement(7788..9467)
                     /gene="ligD"
                     /locus_tag="VNW28_05395"
     CDS             complement(7788..9467)
                     /gene="ligD"
                     /locus_tag="VNW28_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HXA09395.1"
                     /translation="MGLTEYTQKRNFSVTAEPRGAKKPRPKRVAGASRFVIQKHDASR
                     LHYDFRLEMDGVLKSWAVPKGLPWKRGEKHLAVEVEDHPIEYEDFEGVIPAGNYGGGT
                     VMVWDRGNYYVHGEKPLEALAEGRIHLVLEGKKVEGDWTLIRTRSEHGKNQWLLLKSD
                     ADVKPVSKKRDDESVKTGRTMKQIAKDRDAEWKSRRGDDRGEQESFRERVRQAVARNA
                     SGDKKKGATSPIDSLRTAKPRFIAPMKSKLLESPPATGDWIYELKFDGIRALALKQGT
                     KVKLISRNENDLTKKFDEVAVAVAALPGAECAIDGEVVALDEEGRSSFQLLQAREMEG
                     RAAPLYYYVFDLLQLDGKDLTALPLTTRKEMLQQLCSAAGDPIRYSGELGDDAGALLR
                     EVQRRGLEGIIGKRANSVYEPGRRSGSWIKLKCVREQEFVIGGYTPPAGARKHFGALL
                     VGYYEKKKLLFAGKVGTGFDEKWLARLHRKLQGEAREDCPFADLPSKAGGQWTQGITP
                     GMMRKISWVNPVFVCQVKFAEWTRDGKLRAPVFLGLREDKSPHAVRREIAA"
     gene            complement(9624..10805)
                     /gene="glp"
                     /locus_tag="VNW28_05400"
     CDS             complement(9624..10805)
                     /gene="glp"
                     /locus_tag="VNW28_05400"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HXA09396.1"
                     /translation="MTSEAEAIAAVLGRVTALPGATVPLLASLGRFSAQDLLALRPLP
                     AFDNSAMDGYAVQAESCRAGTRLQVIGEQPAGADRGLTMGAGEAIRIFTGAPLPAGAD
                     AVVMQEDVTREAETIVVQVGVEPGEFVRRRGCDLAAGQKILARGEPLAAQTLGLLAAQ
                     GMAEIAVGAVPRVTIVATGDELARPGETPRPGQIFESNSIMLHALTVGAGAKVTMVEH
                     APDKLERLTEILRRGLEADALLVSGGVSVGDRDLVKPALRTLGAEIDLWRVALKPGKP
                     FLFGRVGDCAIFGLPGNPVSAFVTFLKFVRPALRKMMGATEQYFGLRRVTARLAAEVT
                     NDSDRPHYLRGELREGAFRISGRQESHALFGLSRANALLRLAPGDKLAAGMTVAVELW
                     D"
     gene            complement(10802..11155)
                     /locus_tag="VNW28_05405"
     CDS             complement(10802..11155)
                     /locus_tag="VNW28_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09397.1"
                     /translation="MRLPAFILLFCLTSGGPAACLLFAGASCAPKTQPTAGPATHAKP
                     AVIGEVAVVDEEKKFVLIDLAANLYVPAPGTALRATNASGGMAHLRASPEQKRPFIAA
                     DIVDGDPAVGDQVVR"
     gene            11225..11312
                     /locus_tag="VNW28_05410"
     tRNA            11225..11312
                     /locus_tag="VNW28_05410"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11259..11261,aa:Ser,seq:gct)
     gene            complement(11339..12175)
                     /locus_tag="VNW28_05415"
     CDS             complement(11339..12175)
                     /locus_tag="VNW28_05415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HXA09398.1"
                     /translation="MNYEREFRVSPGSRVRLKKLDPQQTDKDEDKHSASRKVARLQKR
                     IDELQFQLFAEDKRSLLICLQALDAGGKDGVIRHVIGSMNPQGCRVASFKQPSAEELA
                     HDFLWRVEQETPRRGEVVIFNRSHYEDVIVVRVRDLVPKSLWSQRYEQINAFEHRLVT
                     SGTHILKFFLHISKEEQLKRFKDRLDDKSRWWKISEADYSDREYWNDYASAYEDVLSK
                     CSTAHAPWYVIPSDHKWFRNLAISQIIADKLEALRIQVPKPTVDIAEIRKKYHAAKKD
                     GR"
     gene            complement(12206..13450)
                     /locus_tag="VNW28_05420"
     CDS             complement(12206..13450)
                     /locus_tag="VNW28_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HXA09399.1"
                     /translation="MMDIPSSAATAIVAIFALTYLGIALGHVPGLKLNRTGIALLGAI
                     AIIVFSGMSTTSVVGLVNWPTILLLFGFFVISAQLRLSGFFGLVANAMAARLDQPARF
                     LFSLMLVTAGLSAFLNHDIVCFVFAPIVATALIRRKINPVPFLIALAIASNIGAAATF
                     VGNPQNMLIAEVAGLDFGSYVLWCIAPVAFALVCAYAIIWTMSRHHLKRAVSLPDDAE
                     QEAQPFDRPHTIKGLIILVLVIAFFFTRIPKEIIALTAAAIHLASPKFRTEDVLKLVD
                     WSILVLFMGLFVVTGAFQATGYGDHAVQCLAHLGLNLQSPSILAVVTATLSNLINNSA
                     AVLLLVKVTDATNPVTAYVLALANSFGGSLLIIGSVSNIIVVQQARELGIKISFRDFA
                     RLGVPVTLAAIAGLLAWVALVT"
     gene            complement(13505..>13858)
                     /locus_tag="VNW28_05425"
     CDS             complement(13505..>13858)
                     /locus_tag="VNW28_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09400.1"
                     /translation="RGLGNGRAWFRLPLWVQKSWGPWTTYGGGGAVVNPAPGQRDYPF
                     GGWLVQRTFGKHLMLGGEVFAQGADTNNDKGFAALNFGGSYNVNNHFSLLFSAGHSVA
                     GDNHTLWYFGFYGTW"
BASE COUNT         2685 a         4406 c         4169 g         2598 t
ORIGIN      
        1 aaaccggaaa ccagcagaac cgccgcggcg cagagaatct tcttcaccgt cccagcgtaa
       61 gcgcccaccc gcccgccgaa aagtccaaag cgcgcgcgcg ggctgccttc gaatcagtct
      121 tgctttcgcc tgggcgtagt cgcgcgaggg aattacgagg ccaaggccat gtttatcgtt
      181 ccatcgacgc ggccaatgct agtccttttg ctaatttttg ctgcaattaa gataaccact
      241 cgatgccaca acgttccctg ggtaagtctg tttccagctt ttcccatccg tctggaattc
      301 tgccgttgtc gtgcgaaaac gtcgcgtccg gggagcgggt cggcgccagg gtggcgcgcc
      361 ggcttgcgct ttagttcgcc agttctaatt tgcccgcgtg aaaaaaatcg aggcgatcat
      421 caagcccttc aaaatggagg acgtgaagga ggccctcgct gagatcggag tcgaaggcat
      481 gacggtgagc gaagtgaagg gcttcggacg gcagaagggg cacaccgaaa tttatcgcgg
      541 cagcgagtac acggtcgatt tcctgccgaa ggtgaaactc gaaatcgtgc tgaccgatga
      601 gctcgcccag cgcgcggtgg gggtcattac cctggcggca aaaaccggca agattggcga
      661 tggtaaggtc tttgtcagcg atgtcgagca gaccattcgc atccggaccg atgaacgcgg
      721 ggcggacgca gtctaggcgc cgggcgcact tattatttct cgcgccgcat gaacgattat
      781 ctcctctccc tcctgctcgg cgtgatcgag ggcctgacgg aattcctgcc ggtcagctcg
      841 accgcccatc tgcggatctc agaggcgctc ttccggatcg acctcggaaa tggcttctgg
      901 aaaatgtaca gcatcgtcat ccaactgggt gcgatcctgg ctttggctgc tttcttctgg
      961 cgtcgcatcc tgaattttct gcgcactttc ccggacggcg aacggggcga ccggacactc
     1021 ttcacccacc cgctcagcct ggtcatgatc gcgtttgtcg tgacggcgat ccctgcgttt
     1081 ctgctcacca agttgatcgg caagaatctc gaaaaccttc acgtcatggc ttgggcgctg
     1141 cttatcggcg ggatcgtgat gtggctggtc gattcgcttt tcgttaggcc aagcgtgcac
     1201 cacatggagg aggcaaatct tccgaccgcg atctggatcg gggcggtgca ggtgctctcg
     1261 gcggtcttcc ccgggacgtc gcgctccatg tgcacgatcg ccgccgggca ggtggcgggg
     1321 atgtcgcgcg aggccgcgct cgagttttcc ttctttctct ccatcccgac gatgtttgtc
     1381 gcgaccgggt atgacctgct caagacgctc aagccgcacc cggggacgga agcgctcgcg
     1441 ccgctcgcca tgacgccgca tctctggatc gtgctcgggc ttgggttcct gacctccttc
     1501 gtggtggcgc tcatggtcgt cgcctggttt ctccactggg tgcgccggca tggattcact
     1561 cctttcgcca tctaccggat tgtgctcggc ctcggcctgc tcgcggcgct gagccgcggg
     1621 atgcttggcc actgactaaa aaggggtaaa ctctccgcac cttgcaaacc gagactgttc
     1681 tacccgacct ccagagctgc gtgctctgcg aggatgtgcg ctgcgaattc aacggcatgc
     1741 agacgcttgt cggcgtgctc aacatcgtgc cgacgccgaa gctgccggtc aattacctgc
     1801 gcctttgcat ctggacgcgc tggtgcagtg gcgcaggccg gtttcatcaa cgctcgaaaa
     1861 tcatcggacc ggacgacacc caaacggtcg cgcaggcgga agtcgatttc gacctgaagg
     1921 aaatggaagg ccatgcgacc aatgtccatt atttcggcgg cgtgcagttc cagcagttcg
     1981 gtctgcacca cgtcgaaatt tacctgggcg acgagttgcg cctgcgtttc ccgttaccgg
     2041 tgatcgaggt caagcaagcc tgacgcccaa agctgccgcc aaggtgggtg agcccggtcc
     2101 tcctgacgag gcgtagaaag aacgccgccg ccttagatcc gccccacgag gacgagaatt
     2161 aggacgatca gcaggaccaa tccgactccg ccggtcggat agtagcccca gccggtgcta
     2221 tagggccagg cgggaaacgc accgatcaaa agcaggatca aaatgatgag aagaatagtc
     2281 cgcatagttc aagcctaacg aagtgttacg tgtttccttc aggatcgtgt tgcaaagcgg
     2341 aaccggcctt ctttttttgg gaaaaacaaa accaacttgc gcccgctggt cgccatccta
     2401 tcgttcgggc gcgatggccg acgacaaccc cggagcgttg ccggcgactc gtcttcaatt
     2461 ggctgcaacg catggcaaac acgatcctcg tcggcgcgca gtggggcgat gaaggcaagg
     2521 gcaagatcat cgacgtgctg accggcaagg ccgacatcgt ggtccgcagc cagggcggca
     2581 acaatgccgg acacaccgtc attcatggcg caaagaaatt cgtcctccat ctcattccct
     2641 ccgggattct gcggcgcggc aagatctgcg tcatcggcaa tggcgtcgtg atcgacccgg
     2701 tcgcgctcgt gagcgagatc gacgggctgc gcaaagcggg gatcaagatc ggaaaaaatt
     2761 tactgatcag cgactgcgcc catctcgtgc ttccgtatca tcgtgcgctc gacgaacagc
     2821 gcgagctgcg caaaggccac gccaggattg gcacgaccaa gcgcgggatc ggcccggcct
     2881 acggcgacaa agccgcgcgc accgggctgc gcatcgccga cctgatacaa ccggctttat
     2941 tctcgcaaaa acttcgcgcc aagatgcgcg agaacaacag catcctgaaa gcgctcggcg
     3001 cccggccgat ttcttttgcg aaggtgaacg ccgaattcct ggccgcgggc gaacgcctgc
     3061 ggccgttcgt cgccaacacg gtcgtctacc tgcacaaggc gttgctacag ggaaaggaaa
     3121 ttctgtttga aggtgcgcaa ggcactttcc tcgacatcga ccacggcaca tatccctatg
     3181 tcacttcgtc gaacacgacg gcgggcggcg cctgcaccgg caccggcgtg gcgccgcatc
     3241 gcatggaccg cgtggtgggt gtgatgaaag cctacaccac gcgcgtcggg gaaggggcct
     3301 tgccgaccga ggacgcgacg atcgccggga tgctgcatga gatgggacgg gaattcggcg
     3361 cgaccacggg gcgggcgcgg cgttgcggat ggttcgatgc cgtggccacg cgctatgcga
     3421 cgatgatcaa cgggatcgac gacctcgcga tcaccaacct cgacggcctc gacagcgtgg
     3481 acccgatcaa agtgtgtgtt ggctatcgcc tcgacgggaa aaaactgaat gtcccaccga
     3541 gcgacgccgc gcagtggaca cgttgccggc cggtctacct gcagatgccg ggctggaaac
     3601 gatccaccga aagagcgaag aacttctccg atctgcctaa cgaagcgcag aactacgtcc
     3661 ggcggatcgc ggaactgacc ggcgcccgtc ttacgatggt gagcgtcggc ccggcgcgcg
     3721 gccagacgct gcatctctag aatcatggcc gcgccgcaca aagcgatccc gcgccatgtc
     3781 gccatcatca tggatggcaa cggccgctgg gccaaagagc gcggcctgcc gcggatcaag
     3841 ggacacgagg aaggcgccaa agcggtcaag gcctgcgtcg aaggctgcgg tgaactcaag
     3901 atcgagttcc tcacgctcta tgccttttcg gccgaaaact ggcggcgccc gaaaagcgaa
     3961 gtcggtgccc tgatgcgctt gctcgagcag ttcctgaagg aaaaaacttc cgagatggcc
     4021 gagaacaacg ttcgcctcca ggcgattggt cgcctgacgg atttgccaaa agcctgccag
     4081 aagcaactgc acaaatcgat cgagcagacc gccaagaata aagggctgac cctcattctc
     4141 gcgctcagtt acggcggccg gctggagatc atcgacggga tcaagagcct gctccgcgcg
     4201 atcgacctcg ggcacatcga ccgcgcgatg atcgacgtcg agatgttcag caaacatctc
     4261 tacacccgct attatcccga tcccgatttg ctcatccgca cctcgggcga aatgcgaatt
     4321 tccaatttcc tcctctggca gctctcctac accgagatgt atgtcacgcc caaactctgg
     4381 cccgactttg acaaggaaga tctctttgcg gcggtcgaag agttcggccg gcgccagcgc
     4441 cgttacggcg ccctgtagtt gtacccgggg cggtgacctc ggacggccgc agcgaaagga
     4501 gaaggcacga caccgcgccg ccggggtcga cgtccccggt tagaggaacg cttccgccac
     4561 cgagtcagcg aggaaacggc cggcgggcgt caggacgtag cgctgcgctc gcgcctgcat
     4621 caatccgagc ctgatgaatt cctccacttc gttaggccga tcatcgaccc attccgctgg
     4681 aacaccccgg ctcgtgcgca gcgacaaggc gacggcttcc gaccgcttca tctgcggcgt
     4741 caggatttcg tcgccggtcg aagccgtttc accggcgaga acacgatcgg cataagcccg
     4801 ataatccgaa atattttgcc agcgccgcag cccgaccgtc gacaccgcgc tcggcccgat
     4861 tccgagataa tcgtggccgg tccagtaggc gcgattatgg accgactgga aaccggggcg
     4921 tgcgtaattc gaaacttcat actgctcata acctgccttg cccaacaaat ccgtcgctct
     4981 cgtgaaaaag tcggcgtccg cttccgggtc ggtccggaat tcgccgcgcg catgccgccg
     5041 gaaaaagtcg gtatcctctt cgtaggtcag gcagtaggcc gaaacatgat ccggcccgag
     5101 cgcgatcgcg cgctgcaacg tcgcctccca ctgggcaagc gtctgaccgg gcaacccgaa
     5161 catcaggtcg atgttcaggt ttttgaagcc gctccgccgc aggaggtcga aagacgcctc
     5221 cgcttgcgcc gcggaatgct cgcgaccaag cagttgcagc agtccgtcat cgaacgattg
     5281 cacgccgagg ctgatccggt tgacgccgtg gtctcgcaag agacccgcct tgcgttccga
     5341 aacgctgccg ggattcgcct cgaacgtcca ctcccgcagc tccgacaaat taagctgcac
     5401 ctgaaagccg cggaacaacg cgtcaagttg cgcggtcgtt agggcggtcg gcgtcccgcc
     5461 gccgaaaaag atcgtttccg ggcgaagagt cgttgtagcc gccgccctgt gggcggcgcg
     5521 ggccagcgtt ccaccgttct cgttaagctc ccgagccatc tcgtgcgttt cgcggagcga
     5581 agcggctacc gtaagctccg ccaggaccgc atcgcaaaac cgggccgcct gggccggatc
     5641 ggcgcgctcc ttgtaaaagg cgcagtaggg acaaatccgc gcgcaaaacg ggatatgcac
     5701 ataaatatgc cgcggcccat ccgccgccgg agggttgtca atcgccttcc cacccataaa
     5761 gtccaccatc gccgcacgcc taatccgctc aatcgctttt ctcttcgccg gtgtcggatg
     5821 gtgcgccgcc gccttcgccc agggccctcc gccgccggct ccgcccccgc ctggaccgac
     5881 cccctccatc gtctatcgcg cttccaatcc ggcggccatc acggactatc gcaccaatcc
     5941 ggaagtcgtt aggcgcatga ccgacgagct cgtcctcgcg gtcacccatc agcccgacat
     6001 cgcgagcgcc tggcgctccc tggtcaagcc taacgaccgg gtcggaatca agatctcggc
     6061 ggcgggcggg gaacttttca ccacccatcg cgatattgtg aacgcgatcg tcgacggcct
     6121 ggtcgcggcc ggacatcaac gccaggacat catcgtctgg gacaaatcgc tcggtggaat
     6181 taaggaaggc ggctacaccc gcggcgaagg ttaccaattg aaatcgatcc cgccgcgcga
     6241 tggctacgac cccaaggcga tcatctccga gccttttctc ggcaacctga tctggggcga
     6301 ttttgaatac gtcggcaaca agggccaggt gccgctgctg agcgacaccg cgaatacgag
     6361 cagcctcagt catttttcca aaatcgtggc gaacgaggtc acgaaaatca tcaacctgcc
     6421 ggtcctgagc gacagcgtgc gcaacggcgt ggccggctgt ctctacaacg tcaccattcc
     6481 caacatcgat aactggcgcc gcttcggcgt gccgccggat ttcggcgcct cgagcatccc
     6541 ggagatttat aacgatccgc acatcggaca aaaagtggtc ctcaacctga tggacggatt
     6601 gcttgcccaa tacgcgggcg gaccgcagtc gcagccgggc tatgccttcc cctttgccac
     6661 gctttacgcg agcaaggacc cggtcgcgat cgattcgatc gccctccggc aactcgagga
     6721 gtggcggaag aaaggaaaac tgccggcgat caagcggatg ggcgcgcacg tcctcatggc
     6781 ggaccagctg ggtttgggca atgcgaatcc agtgcagatc caaatccgcg atgtcggccg
     6841 gtgaaggcga ttctccgatc ggtttcgcgc tcgttttatc tgaccattcg cttcctgccg
     6901 cgcccgctgc gggagccggt cagcctggcc tacctgctgg cgcgagcgac cgacaccatc
     6961 gcggacacgg cggcgatccc ggcggcgacg cgcctcacga cgttgctggc tttcgcccgg
     7021 gtcatcgcgg gcgaaagcga cttcgacgca gtcgcggaac cgctgcgcgc tttcgccgcg
     7081 caacagagcg atccggctga gcacaagttg attgagaatc tccgtggctg catcgattgg
     7141 ctcgcgacgg tgaaccgagc cgaccgcgac gacattcgaa acgttctcca aacgatcgtc
     7201 cggggccagg aactcgatgt ccggagattt ggcgaccccg cggtcgtgac ttccctgcgc
     7261 agcgcggcgg aactcgacga atacactttt ctcgtggccg gctgcgtcgg tgatttctgg
     7321 acgaaactgg gttttcggca cctccggtcg ttcgccagtc gttcgcctaa cgagatggcg
     7381 aaactcggga tcgagtatgg aaaaggcctg caactgatca atgtcctgcg cgatagcggc
     7441 gcggatgcaa agtgcggtcg cggctatttg ccctccgatg aattggccgg ccagccggtg
     7501 gcggaggttt tcgcgcactg gctggaccag gcggaggagc gcctcgaggc cgggattgac
     7561 tactgctccg ccctcacgaa ttggcgaatg cgctttgcga ccgcgttgcc agccttgatc
     7621 ggggcgcgca cgctcaccct cctgcggcaa gccgggcccg aagcgcgagc gcggaaaatc
     7681 aaagtggcac gaaagacggt ccggcggatc ctcgtcgcca gtctggcggc ggccgcttcc
     7741 ccgaccgcct tgcgcgcgct ctttcaacgg ctgctcgaag cacctcgtta ggcggcgatc
     7801 tcgcggcgta cggcgtgagg agatttgtct tcgcgcaggc cgaggaagac cggggcgcgc
     7861 agcttcccgt cgcgcgtcca ctcggcgaat ttcacctgac agacaaagac cgggttaacc
     7921 cagctgattt tgcgcatcat gccaggtgtg atcccctgag tccattggcc gcctgccttc
     7981 gacgggagat cggcaaacgg acaatcttcc cgcgcttcgc cctggagctt ccggtgaagt
     8041 cttgcgagcc atttctcgtc gaagccagtg cccaccttgc cggcgaaaag cagtttcttc
     8101 ttttcgtaat agccgaccag gagcgcgcca aaatgcttcc gcgcgccggc cggcggcgta
     8161 taaccgccga tgacgaattc ctgttcccgc acgcacttga gcttgatcca cgagccgctc
     8221 cgccggcccg gctcataaac ggaatttgcc cgcttgccga tgatgccctc gaggccgcgt
     8281 cgctgcacct cgcggaggag cgcgccagca tcgtccccca gctcgcctga gtagcggatc
     8341 ggatcgccgg ccgcgctgca aagctgctgg agcatttctt tccgcgtcgt taggggaaga
     8401 gcggtcaggt ctttgccgtc gagctggagg agatcgaaga cgtaatagta aaggggcgcg
     8461 gcgcggcctt ccatttcccg ggcctggagg agctgaaagg aggaccggcc ctcttcatcg
     8521 agcgccacca cttcgccatc gatcgcgcac tccgcgccgg gcagggcggc gaccgcgacc
     8581 gcgacctcgt caaatttttt cgtcaggtcg ttttcattcc gcgagatcaa tttgactttc
     8641 gtgccctgct tcagcgccag ggcccggatg ccgtcgaact tcagctcgta aatccagtcg
     8701 cccgtcgccg ggggcgattc gagcagcttc gacttcatcg gcgcgatgaa gcgcggcttc
     8761 gccgtccgga gtgaatcaat cggcgacgtc gcgccttttt ttttatcgcc gctcgcgttg
     8821 cgcgcgacgg cttgccggac gcgctcgcgg aagctttcct gttctcctcg atcgtcaccg
     8881 cgccgcgatt tccattccgc atcgcgatcc ttcgcgattt gcttcatcgt ccggccggtc
     8941 ttgaccgact cgtcgtcgcg cttcttcgag accggcttga cgtcggcatc gcttttcaga
     9001 aggagccact ggttctttcc gtgttcgctc cgggtgcgga tcaatgtcca gtctccctcc
     9061 acttttttcc cctcgagcac gagatgaatg cggccttccg cgagcgcctc gagcggcttc
     9121 tcgccgtgca cgtaatagtt gccgcgatcc cagaccatca ccgttccgcc gccataattg
     9181 cccgcgggaa tgacgccctc gaaatcttcg tactcgatcg ggtgatcctc gacctcgact
     9241 gcgagatgct tttcgccgcg cttccacggc aggcctttcg gcaccgccca cgatttcaag
     9301 acgccatcca tctccagccg aaagtcgtaa tgcagccgac tcgcgtcatg tttttgaatg
     9361 acgaagcgcg acgcgccggc gacccgtttc ggtcgcggct tttttgcgcc gcgcggctcg
     9421 gcggtgacgg agaaattccg cttctgtgtg tattcagtca gacccatctg gacaacgctt
     9481 ccagtaatag acacgaaaac gccatcgcaa tcacgtgggg ttgtgttggt ttatgacgcg
     9541 cggaagtgcg ttcctccgca gagactcacc gcggcgagtc caagctcggc gcaggtggcc
     9601 gggcttccgt ttattgcagt ccgctagtcc cacagctcga ccgccaccgt catccccgcc
     9661 gccaatttgt ctcccggcgc caggcgcagg agcgcgttcg cccggctcag gccgaacagc
     9721 gcgtgcgatt cctgccgccc gctgatccgg aaggcgccct cgcgcaactc cccccgcagg
     9781 tagtggggac ggtcgctgtc gttcgtcacc tcggcggcaa gccgcgcggt cacgcggcgc
     9841 agtccgaagt attgttcggt tgcgcccatc attttccgca aagctggccg gacgaatttc
     9901 aaaaaagtga cgaaagccga gaccgggttg ccgggcagac caaaaatcgc gcaatcaccc
     9961 acgcggccga aaaggaacgg cttccccggc ttgagcgcca cccgccagag atcgatctcc
    10021 gccccgagtg ttcgcaatgc cggtttgacc agatcacggt cgcccactga gacgccgccg
    10081 ctcaccagga gcgcgtcggc ttctaatccg cgccgaagaa tttccgttaa ccgttcgagc
    10141 ttgtcgggcg catgctccac catggtgact ttcgcgccgg cgccgaccgt tagcgcgtgc
    10201 agcatgatcg agttgctctc gaagatctgc cccggtcgcg gggtttcgcc cggccgcgcc
    10261 agctcatcac cagtcgcgac aatcgtcacc cgcggcacgg caccgacagc gatctcggcc
    10321 atcccctggg ccgcgagcaa gcccagggtt tgcgccgcca atggctcgcc gcgcgccaga
    10381 attttctgcc ccgcggccaa gtcgcatccc cggcggcgga cgaattctcc cggctccacg
    10441 ccaacctgca cgacgatcgt ctccgcctcc cgcgtcacgt cctcctgcat gacgaccgcg
    10501 tcggctcccg cggggagcgg agcgccggtg aaaatgcgaa tggcctcgcc cgcccccatc
    10561 gttaggccgc ggtccgcgcc ggccggttgc tcgccgatta cctgcaacct cgtgcctgcg
    10621 cggcacgact ccgcttgcac ggcgtaacca tccatcgccg aattgtcgaa agccggcagc
    10681 gggcggaggg ccaaaagatc ctgcgcggag aaacggccta acgacgcgag caaaggaacg
    10741 gtcgccccgg ggagcgccgt cacgcggccg aggacggccg cgatggcttc cgcctcgctc
    10801 gtcatcgcac cacctggtcg cccacggccg ggtcgccgtc gacgatgtcg gccgcgataa
    10861 acggtctttt ctgttccggt gaggcccgca agtgtgccat cccgcccgag gcgttggtcg
    10921 cccggagtgc cgtccccggc gcgggcacgt agaggttggc agcaagatcg atcaagacga
    10981 actttttctc ctcatccacg accgcgactt cgcctataac cgccggcttg gcgtgggtcg
    11041 ccgggcccgc cgtcggttgc gttttgggag cgcaacttgc gccggcgaag agcaggcagg
    11101 cggctgggcc accgctcgtt aggcagaaca ggagaatgaa ggcaggaagc cgcatgggaa
    11161 ggagtgcaac aggttgacga gcatggaacg gtcccgatat tctcgcaacc tcgacgaccg
    11221 aggaggtgag gtggctgagt ggtcgaaagc agcgctttgc taaagcgccg taccccaaaa
    11281 gggtaccgag ggttcgaatc cctccctcac cgtttgtcct tccacccgcg cgggcgcgct
    11341 atctgccgtc cttttttgcc gcgtgatatt ttttccggat ctcggcgatg tcgaccgttg
    11401 gcttcgggac ctggatgcgg agcgcttcca gcttatccgc gatgatctgc gagatggcga
    11461 ggttgcgaaa ccatttgtgg tcggagggaa tgacgtacca aggcgcgtgg gccgtgctgc
    11521 atttactcaa cacgtcctcg taggcgctcg cgtagtcgtt ccaatattcc cggtcggagt
    11581 agtccgcctc gctgatcttc caccagcgcg atttgtcgtc gagccggtcc ttgaatcgct
    11641 tgagctgctc ctctttgctg atgtgaagga agaatttcag aatgtgcgtg ccgctcgtca
    11701 cgaggcgatg ttcgaaagcg ttgatttgct cgtagcgctg cgaccagagc gatttcggaa
    11761 cgagatcgcg cacgcggacg acgatcacat cttcgtagtg cgagcggtta aagatgacga
    11821 cctcaccgcg ccgcggcgtc tcctgctcga cgcgccagag gaaatcgtgc gctaactcct
    11881 ccgcgctcgg ttgcttgaag ctggcgacgc ggcagccctg cggattcatc gacccgatga
    11941 cgtgacggat gacgccgtcc ttgcccccgg cgtcgagcgc ctgcaggcag atcaggaggg
    12001 aacgtttgtc ttcggcgaag agctggaact gcagctcgtc gatccgcttc tggagtcttg
    12061 cgaccttgcg cgaggccgag tgtttgtctt cgtccttgtc ggtctgctgc ggatcgagtt
    12121 tcttcagccg aacgcggctc cccggtgaga cgcgaaattc cctttcatag ttcatcaacg
    12181 ataagtcagc ctgggcttct gcactctagg tcaccaacgc tacccacgcg agaaggccgg
    12241 cgattgcggc gagggtcacg ggcacgccga ggcgggcaaa gtcgcggaag gaaatcttga
    12301 tccccagttc gcgcgcctgc tggacgacga tgatgttcga gacgctgccg atgataagca
    12361 ggctgccgcc aaagctgttc gcgagcgcga gcacgtaagc cgtcacagga tttgtcgcgt
    12421 ccgtcacttt caccaggagg agcacggccg cggagttgtt aatcaaattg gaaagcgtcg
    12481 ccgtgaccac ggccaggatg gagggggatt ggagattcag ccctaagtgc gccaggcatt
    12541 gtactgcgtg gtcgccgtag cccgtggcct gaaatgctcc cgtgacaacg aagagcccca
    12601 tgaataacac caggatcgac caatcgacca gcttgagcac gtcttcggtg cgaaacttcg
    12661 ggctcgcgag atgaatcgcg gccgcggtca gggcgatgat ttccttcggg atccgggtga
    12721 aaaaaaatgc gatgaccaac acgaggatga tcaatccttt gatcgtgtga gggcgatcaa
    12781 aaggctgcgc ttcctgctcc gcgtcgtcgg gcagcgagac ggcgcgcttc aggtgatgac
    12841 gcgacatcgt ccagataatc gcgtaggcgc aaacgagagc gaaggcgacg ggcgcgatgc
    12901 accaaagcac atacgaccca aaatccaggc cggccacctc cgcaatcagc atgttttgcg
    12961 gattgccaac gaaagtggcg gccgcgccaa tgttgctcgc gatcgcgagc gcgatgagaa
    13021 acggaacggg attgatcttg cggcggatca gcgccgtggc cacgatcggc gcgaagacaa
    13081 aacagacgat gtcatgattc agaaaggccg acagtcccgc cgtcaccagc atcaggctga
    13141 aaagaaaacg cgcaggctga tcgagccggg cggccatcgc atttgcgacc agtccgaaaa
    13201 aaccagagag ccgcagttgc gccgagatca cgaaaaagcc aaacaagagg aggatggtcg
    13261 gccagttgac gagcccgacc acgctcgtgg tcgacatccc ggaaaagacg atgatcgcga
    13321 tcgcaccaag gagcgcgata ccggtgcgat tgagtttcaa tcccggcaca tgcccgagcg
    13381 cgatgccgag ataagtcagc gcgaagatcg ccacgatggc cgtcgcggcc gaggaaggaa
    13441 tgtccatcat gcgtccacct gcgatcggag ctgcacattt ccagggaagt ttttcattcc
    13501 ctccttacca ggtgccgtag aacccgaaat accaaagcgt gtggttgtcg cccgcaacac
    13561 tgtggccggc gctgaagagc aggctgaaat gattattcac attgtaggag ccgccgaaat
    13621 tcagcgcggc aaacccctta tcattgttcg tgtctgctcc ttgcgcgaaa acttcgccgc
    13681 caagcatgag gtgtttgcca aaggtacgct gcactaacca accgccaaaa gggtaatcac
    13741 gctggcccgg ggcgggattt accaccgcgc cgccgccgcc ataagtcgtc cacgggcccc
    13801 agcttttctg cacccacaaa ggcaggcgaa accacgcccg tccgttgccc agtccgcg
//
LOCUS       DATMNS010000155         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2724393, whole genome shotgun sequence.
ACCESSION   DATMNS010000155 DATMNS010000000
VERSION     DATMNS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2724393"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VNW28_05430"
     CDS             <1..820
                     /locus_tag="VNW28_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXA09401.1"
                     /translation="MFHLFTHAAFKALLFLGAGSIIYMLHHEQDIWRMGGLAKRLPIT
                     FLTFLVGTLALIGFPGFSGFFSKDAILALAYERNLPIFVFATFTALLTAFYMTRLMVV
                     AFFGNARSPHAARGAESPLVMTLPLILLSVPAAIGGFGFFARNFLSLPNESKPVAAVP
                     VIAFLALLIGVVSALLLYRNRTTEPVEIELFRNRFYFDELYAALIRATQGLLAAIAAF
                     VDRWILDGAIVRGLSGGIWGGGFLLRLLQVGNLQAYGFLFGLGIIGLIYFTVFH"
     gene            820..2301
                     /locus_tag="VNW28_05435"
     CDS             820..2301
                     /locus_tag="VNW28_05435"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXA09402.1"
                     /translation="MLLFIVFAPLVTALLILLGAPARLTALLGAAATVGTTLAGLFLF
                     DPVRAGFQFVSSFPISASWRLQFLLGADGLSLLMLFLAALVLLCAVWFTPPIEKSERL
                     FYACLLFLATGAIGAFASLDLFFFYAFHELALIPTFLLIGIWGSGNRFAAAWKVTIYL
                     GLGSFVLLLGLILLYRSAPPESRSFDLRALQAVAANGGITLHAQNQIFFLLLIGFGVL
                     ISLFPFHTWAPEAYASAPAPAAMLHAGVLKKFGLYGLLRVALPLLPDGAHHWNTLLVV
                     LLLGNIIYVGLVTLAQKRLDWMLGYSSVMHMGYIFLGIASANVIGLNGAAVLMFAHGL
                     SIALLFALAGEIRRRTGTLVLSDLGGLGKVMPFAGFAFGVATFAAIGLPGFANFAAEV
                     MVFFGAFRVGADWQGFHLLQVATLLALWGVVISAVYMLRAYRSIFMGEINDRWKNLPD
                     LARTLRLPVILLVAASLLVGFLPNTVLRVLRPTFPAPVAATHR"
     gene            2298..3758
                     /locus_tag="VNW28_05440"
     CDS             2298..3758
                     /locus_tag="VNW28_05440"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HXA09403.1"
                     /translation="MIPAPDLEIAVLALGLILLMVDAFAGQTNRRGVALAGILGLLVV
                     LAASFFLSPPAPAAASGFWSFYTADPLAIFFKRFALVTTILVLVMAMDYAPAIRMGVP
                     AANPHAGLGEFFTLPIFTCAGLMWMASAIDFVMIFVSLELVTVSFYVLVSFTRRNPAT
                     LEAGVKYLILSALSTGFLVYGITWIFGVTGETNLTQIGTVLATQEIDKIPLLFGIALV
                     LVALGFKIAAVPFQIWVPDVYQGAPTPVTAFLSVGSKAAGFVVLLRVLTPLLMLPQVG
                     RLLVGVAALTLIYGNFAALPQTNLKRLLAYSSIAHAGYLLIGIVTLSGPAIAYYLVAY
                     LLMTLLCFAVLVVVGKSGGSDQIEDYAGLAKRSPFLAFAMLIGMISLAGVPFTAGFLG
                     KFFIFYAAIGQHQTMLVVIGVITVGCGFYYYLKVVRAMYWLTPPADSQPVPLSLFSRL
                     TMSLLIAAIFVLGVYPQPILNALDQPAARATLSMSR"
BASE COUNT          535 a         1278 c         1085 g         1004 t
ORIGIN      
        1 gatgtttcat ctcttcaccc acgcggcctt caaggcgctc ctcttcctcg gcgccggctc
       61 gattatttac atgctgcatc acgaacagga catctggcgc atgggcggac tcgcaaagcg
      121 gttgccgatc acattcctca cttttctcgt cggcacgctc gcgcttatcg gtttccccgg
      181 cttcagcggc ttcttcagca aagacgccat cctcgctctc gcctacgaac gcaacctgcc
      241 gatcttcgta ttcgccactt tcaccgcgct gctcaccgcc ttttacatga cacgactgat
      301 ggtggtcgca tttttcggca atgcgcgcag cccgcacgcc gcccgcggcg cagaatctcc
      361 ccttgtcatg acgctccccc tcattctcct cagtgtcccg gcggcgatcg gcgggtttgg
      421 tttttttgcc cggaatttcc tcagcctgcc taacgagtcg aaaccggtcg ccgccgtccc
      481 cgtgatcgcc tttctcgcgc tcctcatcgg ggttgtcagc gcgctcctcc tttatcgcaa
      541 ccgcaccacc gagccggtcg agatcgaact gttccggaac cgattttatt tcgatgaact
      601 ttacgccgcg ctcatcaggg cgacgcaggg cttgctcgcg gccatcgccg cgttcgtcga
      661 tcgctggatt ctcgacggcg caatcgtgcg cgggttgagc ggcggaatct ggggcggcgg
      721 atttctgctc cggttgctcc aggtcggcaa cctgcaggct tacggttttc tcttcgggct
      781 cgggatcatc ggactcatct acttcaccgt tttccactga tgctgctctt catcgtcttc
      841 gcgccgctgg tcacagcact gcttattctc ctcggcgcac cggcgcggct gaccgccctg
      901 ctcggtgcgg cagcgacggt ggggacgact ctggccggcc tttttctttt cgacccggtg
      961 cgcgccgggt tccagtttgt cagttcgttt ccgatcagcg caagctggcg gctccagttc
     1021 ttgttaggcg ccgacgggct cagcctgctc atgcttttcc tcgccgcgct tgtcctcctt
     1081 tgcgccgtct ggtttacccc gccgatcgaa aaatccgaac gcctgttcta tgcctgtctc
     1141 ctttttctcg cgaccggcgc gatcggtgcc ttcgcttcgc tcgatctttt tttcttctac
     1201 gcctttcatg agcttgcctt gatcccgact tttcttttga tcggcatctg gggatcgggc
     1261 aaccgtttcg ccgcggcttg gaaagtaacg atctatctcg gtctcggcag tttcgttttg
     1321 cttctcggct tgatcctgct ctaccgcagc gcgccgccgg aatcgcgcag cttcgatctg
     1381 cgcgcgctgc aggccgtggc cgccaatggc gggattacct tgcacgcgca aaaccagatc
     1441 ttctttctcc tgctcatcgg gttcggcgtt ttgatttcgc ttttcccgtt tcacacctgg
     1501 gcgcccgaag cctacgcctc cgcgcccgcg ccggccgcca tgctgcacgc cggtgtcctg
     1561 aagaaattcg gcctttacgg gcttctccgc gtcgcgctgc ccttgctgcc cgatggcgcg
     1621 caccactgga acacgctgct cgtcgtgctc ttgctgggca acattatcta tgtcggactc
     1681 gtcaccctcg cgcagaagcg gctcgattgg atgctcggct attcgagcgt gatgcacatg
     1741 ggctacattt ttctcgggat cgcgagcgcc aacgtgatcg gtctgaatgg cgccgccgtg
     1801 ctcatgttcg cccacggcct ttcgatcgcg ctgcttttcg ccctggccgg agagattcgc
     1861 cggcgcaccg gcaccctggt gctgagcgat cttggcgggc tcggcaaggt catgcctttc
     1921 gccggctttg cctttggggt cgcgactttt gccgccatcg gccttcccgg tttcgccaat
     1981 tttgcggcgg aagtgatggt cttcttcggc gcgtttcgcg tcggcgcgga ctggcagggc
     2041 ttccaccttt tgcaggtcgc cacgttgctc gcgttgtggg gcgtggtgat ttccgccgtc
     2101 tacatgctgc gcgcctaccg gtcgattttt atgggcgaaa taaacgatcg atggaaaaac
     2161 ctgcccgatc tcgcgcgcac attgcgtctg ccggtcatcc tgctcgtcgc cgcctcattg
     2221 ctcgtcggct tcctgccaaa caccgtcctg cgcgttctgc gcccgacctt ccccgcgccg
     2281 gtcgccgcca cccaccgatg atcccggccc ctgatctcga aattgcggtt ctcgccttgg
     2341 gattgatcct gctgatggtc gatgccttcg ccggccaaac caatcggcgc ggcgttgcgc
     2401 tcgccggaat tctcggtctt ctcgtggtgc tcgcggccag cttcttttta agtccgcccg
     2461 cgccggctgc ggcgagcggt ttctggagtt tctacactgc ggatccgctc gccattttct
     2521 ttaaacgctt cgcgctcgtg acgacaattc tcgttctcgt gatggcgatg gattacgctc
     2581 ccgcgattcg catgggcgtg cccgcagcga atccgcacgc cggtctcgga gagtttttca
     2641 ccctcccgat ttttacctgc gccggcctga tgtggatggc gtccgcgatc gatttcgtga
     2701 tgatcttcgt ctcgctcgag ttggtcacgg tgtcgttcta tgtgcttgtg agttttaccc
     2761 ggcgaaatcc cgcgacgctc gaggccggag tgaaatatct gatcctgagc gcgctttcaa
     2821 ctggctttct cgtctacggc atcacctgga tttttggcgt gacgggtgag accaacctga
     2881 cgcagatcgg gaccgttctg gccacgcagg agatcgacaa gatcccgctt ctgttcggca
     2941 tcgcccttgt ccttgtcgcg ctcggtttca aaatcgccgc ggtccctttt caaatttggg
     3001 tgccggacgt ttatcagggg gctccgacac cggtgacggc cttcctttcg gtcggctcga
     3061 aggccgccgg cttcgtcgtc ctgctgcgcg tgctcacgcc gttgttgatg ctcccgcaag
     3121 ttggccggct gctcgtcggc gtcgctgctc tcaccctgat ctacgggaac ttcgccgccc
     3181 tcccgcaaac aaatttgaaa cggctgctcg cctactccag catcgcgcac gccggctatc
     3241 tgcttatcgg cattgtcacg ttgtccgggc cggcgatcgc ttattatctg gtggcgtatc
     3301 tgctcatgac gctgctttgc ttcgccgtgc tggtcgtcgt cggaaagagc ggcggaagcg
     3361 atcagattga ggattacgcc ggcctcgcaa agcgctcgcc cttccttgcc ttcgcgatgt
     3421 tgatcgggat gatctccctc gccggagttc catttaccgc cggcttcctc ggaaagtttt
     3481 tcatcttcta cgccgccatc gggcagcacc agaccatgct cgtggtgatc ggtgtgatca
     3541 cagtcggctg cgggttctat tattatctga aagtggtgcg cgcgatgtac tggctgaccc
     3601 cgcccgccga ttcccagcca gtgccgttga gcctgttctc gcgcctcacg atgtcgctct
     3661 taatcgcggc gatctttgtc ctgggtgttt atccgcagcc gattttgaat gcgctggacc
     3721 agccggcggc gcgtgccacc ctctcgatgt ctcgttaggc gcgacgctgt cgggaacgct
     3781 cgcaatgttc ggcctgcggc aacacttcct tcctagtgca ccggaccaga tgtttcgtga
     3841 gtgaggatga tatacgtgag tcacgtatat catcaaatgc acggtcggag cgcgaggtca
     3901 tc
//
LOCUS       DATMNS010000156         7293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3691549, whole genome shotgun sequence.
ACCESSION   DATMNS010000156 DATMNS010000000
VERSION     DATMNS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7293
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3691549"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VNW28_05445"
     CDS             <1..611
                     /locus_tag="VNW28_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXA09404.1"
                     /translation="VLGFVLMLTQAFFYNAIFFTYPLVLLRFYAVPAANAGLYLLPFA
                     LGNVLGPVVIGHFFDTVGRKPMITLTYIVSGLLLALTGWLFHAGLLDARTQTIAWMVI
                     FFIASAAASSAYLTVSEIFPLEVRAFAIAVFYAVGTLAGGVGAPLLFGWIIGTGSKTA
                     LLIGYLVGAALMIIGGLTEAWLGVAAEGRSLESVAAPLSTRG"
     gene            complement(823..1494)
                     /locus_tag="VNW28_05450"
     CDS             complement(823..1494)
                     /locus_tag="VNW28_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HXA09405.1"
                     /translation="MNAYLDRLDEQIAARHLLTHPFYLAWTRGELHKATLADYACQYY
                     RHVAAFPTYLSAMHARCDDQPTRRQLLANLIDEEAGDPNHPQLWRNFAATLGVSPNEL
                     DDTATWPETTDLIATFRSVCGEGDLAAGLAALYAYESQIPAVSESKIDGLIRHYYFDD
                     PESYRYFTVHIEADREHAAMERVLLASHVRPENADAVTTAADRVLNALWELLSGVCRR
                     HDLAC"
     gene            complement(1478..2209)
                     /locus_tag="VNW28_05455"
     CDS             complement(1478..2209)
                     /locus_tag="VNW28_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXA09406.1"
                     /translation="MTNHPEISVIIPVANEAAVLPACLARIAGSPHEIIVVDAASDDQ
                     TVVLAEAAGCRVVPAGQRHRARQMNLGARQARGRILLFLHADTWLPPNALPRIIEAID
                     RRHALGGGFARRYRSSSPTLALSSRLAGLRNRIWGWHLGDQAIFVRLDIFEQSRGYRD
                     LPIFEDLDFSRRLRSLGKTVTLTPPVYSSARRFVAKGPLRTTWHDLLLTRQYLLGGDS
                     NDPPQADCQPRDDRLKALQTHERLS"
     gene            complement(2241..2909)
                     /locus_tag="VNW28_05460"
     CDS             complement(2241..2909)
                     /locus_tag="VNW28_05460"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HXA09407.1"
                     /translation="MVSKLSLLPLSLLIALGAAQAAPLTPADTKALLGRIRAKRAAAP
                     QVQADFQEEKAVHLMNKPINSSGKVWFAAPDKFRREVKGNSPSLTVSDGQQLWIYYPK
                     FQSAEHYSLGKHSPLDAGVAALTAALNLQNVENSYHITGSATANGYQLQLAPRNPSLK
                     RLLQMFTIQLNADLQVQRTEMAQPNGDHIVTVYANESRAAIDPTIFEFTPPPGTNMTT
                     PLGH"
     gene            complement(3025..5526)
                     /locus_tag="VNW28_05465"
     CDS             complement(3025..5526)
                     /locus_tag="VNW28_05465"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HXA09408.1"
                     /translation="MSYSKFIARLVTQHRSLVWLVVALLAASSLFILITRLTLDTEVL
                     NLLPGKFESVQGLKVYNRDFAQTRELTFALVSQPNDVDRLEEFAPIFAERLRQQPWST
                     RVLSGSPIETPEAIHDLQGLAVPLLLNLEPRAFEETVAILQPARIATRLSRLRQEIEA
                     GSPRTDFELSLDPLGIITPALKPFAGNDALQQGQPLASPDRTTRIFLAVTNQPSISAF
                     DCQALMKKVNAFRATASQGWEGEAPLQVLVTGRSAYVAEISLSMRHDIVVTLLGSILL
                     VGGVFFVGFRRWLPLVGMGFSLLLCCLVALAVGLLIFGELNMVTIGMCAILIGLGVDF
                     AILIFGRYQQARTEGRTHPAAIEEAVAKLGRAIFFGALTTAVGFLALLLAGSAGFTQL
                     GVLIAVGILVAGLFMTSVFFLFTGQRSVPPRHDWILALVKLFVRRMLRAPGLILWIAL
                     PILLLLFAVAVAPKPPLIFDASAHSMEPKSSDAGFALKTIMAKMPVRWEPVIGIVRAP
                     NEEQLHADWQKLEAHWRNLQKTGAIKSFSTPAALALSPRLLEQNRLRLRTIDFPAARQ
                     ALEKAIAQDGFSRATFVAGFALLDQLEAVARGTEPIPDWRKQLPKTSSWWFLVDRYFA
                     HDPLLTVGFVTTNQPVETQNHKEMLERELPVAGVPLILSGWSFTLTDLIPWSHRQLIL
                     ISVLMAFFDASLLAFLYRDWRLWLIQIVTLALAIGAMIATMKLAHIPLNLLNVLAFRL
                     VLAIGVDYGIYVLLVWQKAREIEHDVAGVLKPVALAGLTAIAGFGSLGFANNPTLSGL
                     GIACAIGLAWSLFATIFFTLPAAVAAKPKTVESGR"
     gene            complement(5542..5961)
                     /locus_tag="VNW28_05470"
     CDS             complement(5542..5961)
                     /locus_tag="VNW28_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09409.1"
                     /translation="MRPFLFAGAIAAFFSLTSCQTLRHQFVQPEADWQAKIGQLQYRG
                     PKTSLIGDVLVRYSTKGDFELTFSKGPGVTLLTVHQDDKFVRVSGPLAHGSWSGQPNE
                     APARLRGWVSLRALLLRAKTQPLIRQTIGPDRFTFAF"
     gene            complement(5968..7272)
                     /locus_tag="VNW28_05475"
     CDS             complement(5968..7272)
                     /locus_tag="VNW28_05475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXA09410.1"
                     /translation="MSDAIYDVAIIGGGPAGSTAATLLARAGRRVIVCEREKFPRFHI
                     GESLLPISMQTFTRLGVHEKFERAGFMRKYGGEMASACSDEGVKFYFKDGYRSQTETS
                     YQVPRAEFDQVLLDHAAENGAEVREQTSVKGVEFFADRAELTVQGKEAAPEKISARYV
                     IDASGRHSVLGNHFKLKEAYAHLQKISIFAHYEGVQADEGRDGTLTRQLRAADRWFWY
                     IPLPRDRASIGVVLDTALFKQAKKSPEDFLGEALAEQPFLARRMTHARRITPVYASAD
                     FSYRQTRLTGARWLLAGDAAGFIDPVFSSGVFLALLGAEQCADTLDIVLDYPRRASRL
                     FAQYERLLRRVMQGYLRFVNAWYSREFIEVFLYPQDVLQIPPAVNAVLGGNISGSFAI
                     RWRMSAFYFIVWLQRYFPLCPRRTLVAKKDGVLHPESLRPAA"
BASE COUNT         1381 a         2147 c         2465 g         1300 t
ORIGIN      
        1 cggttctcgg cttcgtcctc atgctcacac aggccttttt ctacaacgca atcttcttca
       61 cctacccgct cgtcctcctg cgcttctacg ctgtgccggc ggcgaacgca gggctttacc
      121 tgctgccctt cgcgctcggc aacgtcctcg ggccggtcgt catcggacat ttcttcgaca
      181 ccgtcgggcg caagccgatg attacactta cttacattgt ttccgggcta ctcctcgcgc
      241 tcaccggctg gctctttcac gccggcctgc tcgatgcccg cacccagaca attgcctgga
      301 tggtgatctt ctttatcgct tccgccgcag ccagctccgc ctacctcacg gtcagcgaaa
      361 tcttcccgct ggaagtgcgc gcgtttgcga tcgcggtctt ttacgccgta gggacgctgg
      421 ccggcggagt cggcgcgccg ctgctcttcg gctggatcat cggtaccgga tcgaagacgg
      481 cgctccttat cggctatctt gtcggcgcgg ccttgatgat catcggaggc ctgacggaag
      541 cgtggctggg cgtggcggcc gaggggcgtt cgctcgagag tgtggctgcg ccgctctcca
      601 cgcgcggatg agccaatcgc caaagggagg catccgcggc ggaagaattg ccgggcgagg
      661 cttggtgcgt ccattgacct gccgcagcgg tagacaagct tctccccttc gatccgattt
      721 cgtggcggcg agtcgccggc accagcacgc gggtcgcgtg cgctccccgg agtctgaacg
      781 caggctctga ccgacttcaa cgttgcaccg taccagcgcc gcctagcagg cgagatcgtg
      841 gcgccggcaa actcccgaga gcagttccca aagcgcgttg agcacgcgat cggcggcggt
      901 cgtgaccgcg tccgcattct ccggccgcac gtgcgaagcg aggaggactc tctccatcgc
      961 ggcgtgttcg cggtcggcct cgatgtggac ggtgaagtag cggtaacttt ccgggtcgtc
     1021 gaaatagtag tgccggatta gcccgtcgat cttcgactct gagaccgccg gtatctggct
     1081 ttcgtaggcg taaagcgcgg cgagaccagc ggcgaggtca ccctcgccac aaacggaacg
     1141 aaaagtcgcg atcaagtccg tggtttccgg ccaggtcgcg gtgtcgtcga gttcgttagg
     1201 cgagacgccg agggtggcgg cgaagttccg ccagagctgc ggatgattcg ggtcgcccgc
     1261 ttcctcgtcg atcaggttgg cgagcaactg gcggcgggtc ggttgatcgt cgcagcgcgc
     1321 atgcatggcg gagaggtagg tcggaaaagc ggcgacgtgt cggtaatact ggcaggcata
     1381 gtccgccagc gttgccttgt gcaattcacc ccgcgtccag gcgaggtaaa acggatgggt
     1441 caggaggtgg cgggcggcga tttgttcgtc aaggcgatca agataggcgt tcatgagttt
     1501 gcagagcttt tagacgatca tcgcgcggtt ggcaatccgc ctgaggcgga tcattcgaat
     1561 ctcctcccaa caaatattgg cgggtcagaa gcagatcgtg ccaagtcgtg cggagcggcc
     1621 ccttggccac gaagcgccgg gccgaggagt aaaccggtgg cgtcagcgtc acggtcttgc
     1681 cgagagaccg cagccgccgc gaaaaatcga ggtcttcgaa aatcggcagg tcgcggtagc
     1741 cgcgcgattg ttcgaaaata tcgagccgca cgaaaatggc ctggtcgccg aggtgccagc
     1801 cccagatacg attacgcagg ccggccagcc ggctgctgag cgcgagagtg ggcgaggatg
     1861 aacgataacg ccgggcgaat cctccgccga gggcgtggcg ccgatcgatc gcttcgataa
     1921 ttcttgggag agcgttggga ggcaaccagg tgtccgcgtg cagaaaaagc agaatgcggc
     1981 cgcgcgcctg ccgggcgcca agattcatct gccgcgcgcg atgacgttgc cccgccggca
     2041 ccacgcgaca acccgcggct tctgcgagca cgaccgtctg atcgtcgctc gcggcatcga
     2101 ccacgatgat ctcgtgaggc gagccggcga tgcgtgcgag gcatgccggc aggacggcgg
     2161 cttcattcgc gaccggaatg atgaccgaga tttcaggatg gttggtcacc ggcattttca
     2221 cggtgcggct accgcgggtt tcaatgtccc agcggcgtcg tcatgttcgt gcccggcggc
     2281 ggggtgaact cgaaaatcgt gggatcgatc gcggcgcgcg attcattcgc atacacggta
     2341 acgatgtggt cgccgttagg ctgggccatc tccgtgcgct gcacctgcag atcggcattg
     2401 agctggatgg tgaacatctg gaggaggcgc ttgagcgacg gattgcgcgg ggcgagctgg
     2461 agctggtagc cgttcgccgt cgcgctcccc gtgatgtggt agctgttctc gacgttttgc
     2521 aggttgagcg ccgcggtcag agcggcgacc ccggcatcga gcggggaatg tttccctaac
     2581 gagtaatgtt ccgccgactg gaatttcggg tagtaaatcc agagctgttg cccgtcgctt
     2641 accgtcaggc tgggcgagtt gcccttgacc tcgcggcgaa atttgtccgg ggccgcgaac
     2701 cagactttgc ccgagctgtt gatcggtttg ttcatgaggt ggacggcttt ttcctcctgg
     2761 aaatcggcct gcacctgggg cgcggcggcg cgttttgccc ggatgcggcc gaggagcgct
     2821 ttcgtatcgg cgggcgtgag cggagcggcc tgcgcggcgc cgagcgcgat caggagcgag
     2881 agggggagca aagagagttt ggagaccatg tttcttgagc aggacttccg tcgcgctgag
     2941 gtattcggaa agaatgaatc aggaaagcag gaaaggaatc ggacattcgc catccaagtt
     3001 cacggctgca agttcttcct gctcctacct tcctgattcg acggttttcg gtttggccgc
     3061 gacggccgcg ggcagggtga aaaagatagt ggcaaagagg ctccaggcga ggccgatcgc
     3121 gcaggcgatg ccgaggccgc tcagcgtcgg attattggcg aagcctaacg aaccgaagcc
     3181 ggcaatcgcg gtcaggccgg cgagcgcgac cggcttcagg acgccggcca cgtcatgctc
     3241 gatctcgcgc gctttttgcc agacgagcag gacgtagatg ccgtaatcga cgccgatcgc
     3301 gagcaccagc cggaaggcga gcacgttgag cagattcagc gggatgtgtg cgagcttcat
     3361 ggttgcgatc atcgcgccga tggcgagcgc gagggtcacg atttggatca accagagccg
     3421 ccagtcgcga tagaggaagg cgaggagcga ggcgtcgaaa aaggccatca gaacgctgat
     3481 caagatcagt tggcgatgcg accacgggat caggtcggta agcgtgaagc tccatccgga
     3541 gagaatcagc ggcacgcccg cgaccggcag ttcgcgctcc agcatttcct tgtggttttg
     3601 cgtctcgacc ggctggttcg tggtcacgaa gccgaccgtc agcaacggat cgtgcgcgaa
     3661 atagcgatcg accaggaacc accaactgga cgtttttgga agctgcttgc gccaatcggg
     3721 aatcggctcc gtgccgcgcg cgaccgcctc cagttgatcg agcagggcaa agccggcgac
     3781 aaaagtcgcg cggctgaatc cgtcctgcgc gatcgccttc tccagggcct ggcgggctgc
     3841 cggaaaatcg atcgtgcgca gccgaagacg attttgctcc agcaggcgtg gcgaaagagc
     3901 gagcgcggcc ggagtcgaga aactcttgat cgccccggtc ttttgcaggt tgcgccaatg
     3961 cgcctcgagt ttttgccagt cggcgtgcaa ttgctcctcg ttaggcgcgc ggacgatccc
     4021 gatgaccggc tcccagcgga ccggcatctt cgccatgatc gtcttcaggg caaagccggc
     4081 gtcgctcgac ttcggctcca tcgaatgggc gctggcgtcg aagatcaacg gcggtttcgg
     4141 cgcgaccgcg accgcgaaaa ggaggagcag aatcgggagc gcgatccaga gaatcaaacc
     4201 cggcgcgcgc agcatgcggc gcacgaagag cttgacgagg gcgaggatcc aatcgtgccg
     4261 cggaggaacc gagcgttgcc cggtgaagag aaagaaaacg ctcgtcatga aaaggccggc
     4321 tacgaggatg ccgaccgcga tcagaacgcc gagctgagtg aagccggccg agccggcgag
     4381 aagaagcgcg aggaagccaa ctgcagtcgt gagagcgccg aagaaaatgg cgcggccaag
     4441 tttggcgacc gcttcctcga tcgccgccgg gtgcgtgcgg ccttcggtcc gggcctgttg
     4501 gtagcgaccg aaaatcagga tcgcaaaatc gaccccgagc ccgatcagga tcgcgcacat
     4561 cccgatcgtc accatgttga gctcgccgaa aatgaggagc ccgacggcga gggcgacgag
     4621 gcagcagagg aggagcgaaa atcccatccc gacgagcggg agccagcggc ggaagccaac
     4681 aaagaagacg ccgccgacaa gcaggatcga gcccagcagg gtgacgacga tgtcgtgccg
     4741 catgctcagt gaaatctccg cgacgtaggc ggaacggccg gtgaccagga cctggagcgg
     4801 cgcttcgccc tcccagccct gactggccgt ggcgcggaac gcgttcactt ttttcatcaa
     4861 tgcctggcaa tcgaaggcac tgatcgacgg ctggttggtg accgcgagaa agatgcgcgt
     4921 ggtccggtcg ggcgaggcga gcggttgccc ttgctggagc gcgtcgttgc ccgcgaaggg
     4981 tttgagcgcg ggggtgatga tgcctaacgg atcgagggac agctcaaaat ccgttcgggg
     5041 cgagccggcc tcgatctcct ggcgcagccg gctgagccgc gtcgctattc tggccggctg
     5101 gaggatggcg acggtctcct cgaaagcgcg cggctcgaga ttgaggagca ggggaacggc
     5161 cagcccctgc aggtcgtgaa tggcttccgg cgtctcgatc ggcgaaccac tcaagacgcg
     5221 cgtgctccag ggctgctggc gcaaccgttc cgcgaagatc ggcgcaaact cctccagccg
     5281 gtcgacgtcg ttaggctggc tgacgagcgc gaaagttagc tcgcgcgtct gcgcgaaatc
     5341 gcggttgtaa accttcagcc cctggaccga ttcgaatttt ccagggagga ggttgaggac
     5401 ttcggtgtcg agcgtcagcc gggtgatgag aataaagaga ctgctggccg caaggagcgc
     5461 gaccacgagc cagacgaggc tgcgatgctg cgtgacgagg cgggcgatga acttcgaata
     5521 cgacatcaac gaccgcgccg gtcagaaggc aaaggtaaag cggtcggggc cgatggtctg
     5581 gcggatgagc ggctgggttt ttgcgcggag gagcagggcg cggagcgaga cccagccacg
     5641 caggcgggcg ggcgcctcgt taggctggcc ggaccacgaa ccgtgggcga ggggaccgct
     5701 cacccgcacg aacttgtcgt cctggtgcac ggtcaaaagg gtgacgccgg ggcccttgct
     5761 gaaggtgagt tcaaagtcgc ccttcgtgga ataacgaacc agcacgtcgc cgatgagcga
     5821 cgtcttcggc ccgcggtact ggagctgacc gatctttgcc tgccaatccg cctccggctg
     5881 gacgaactgg tggcgcaacg tctggcagct cgttaggctg aagaaggccg cgatcgctcc
     5941 ggcaaaaaga aatggccgca tgattcgtta ggcagccgga cgcagcgatt ccgggtggag
     6001 caccccgtcc tttttcgcga cgagcgtgcg ccgcgggcag agcgggaaat atcgctgcag
     6061 ccagacgatg aaatagaagg ccgacatccg ccagcggatc gcgaaactgc cgctgatgtt
     6121 gccgccgagg acggcgttca ccgccggcgg aatctggagc acgtcctgcg ggtagagaaa
     6181 gacctcgatg aattccctcg agtaccaggc gttgacgaaa cggagataac cttgcatcac
     6241 ccggcggagc agccgctcgt actgggcgaa aaggcgcgat gctctgcgcg gataatcgag
     6301 cacgatgtcg agggtgtcgg cgcactgttc ggcgccgagc agcgcaagaa aaacgccgct
     6361 gctgaagacc gggtcgatga aaccggcggc gtcccccgcg agcagccaac gggcgcccgt
     6421 gaggcgggtt tggcgatagg aaaaatccgc gctcgcgtag acgggcgtga tacgccgggc
     6481 gtgcgtcatg cgccgggcaa gaaacggctg ctcggccagc gcttccccga ggaagtcctc
     6541 cggggatttt ttcgcctgct tgaagagcgc ggtgtcgagc acgaccccga tgctcgcgcg
     6601 atctcgcggg agcgggatat accagaacca gcgatccgcc gcgcgcaact ggcgggttag
     6661 cgtgccgtcg cgcccttcgt cagcctgcac gccttcgtag tgggcgaaga tcgagatctt
     6721 ctggaggtgc gcgtaggctt ccttgagctt gaaatgattc ccgagcacgg aatggcgccc
     6781 gctcgcgtcg atcacataac gggcgctgat tttttccggc gctgcctcct tgccctgcac
     6841 cgtgagctcg gcgcggtcgg cgaaaaactc gactcctttg accgatgttt gctcgcgcac
     6901 ttcggcgccg ttttccgcgg cgtgatcgag cagcacctgg tcgaattccg cccgcggcac
     6961 ttgatatgag gtttcggttt gcgagcggta gccgtccttg aaataaaact tcactccttc
     7021 atccgaacag gcgctggcca tttcgccgcc gtatttccgc atgaacccgg cccgctcgaa
     7081 tttctcgtgc accccaagac gggtgaaggt ttgcatgctg atcgggagga gtgattcgcc
     7141 gatgtggaag cggggaaact tttcccgctc gcaaacgatc acgcgccggc cggctcgcgc
     7201 gaggagggtc gccgcggtgc tcccggcggg tccaccaccg atgatcgcga cgtcgtagat
     7261 tgcgtcagac attcggcgat caagttagca agc
//
LOCUS       DATMNS010000157         9555 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1653104, whole genome shotgun sequence.
ACCESSION   DATMNS010000157 DATMNS010000000
VERSION     DATMNS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9555
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1653104"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /gene="folE"
                     /locus_tag="VNW28_05480"
     CDS             <1..330
                     /gene="folE"
                     /locus_tag="VNW28_05480"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HXA09411.1"
                     /translation="EHHLLPFIGRAHVAYVPDGRVIGLSKVARIVDVFARRLQIQEQL
                     TTQVADALMRSLHPLGVAVVIEAKHLCMMMRGVEKQNSIMKTSCLLGVFKEDARTRSE
                     FLALLGQ"
     gene            337..1593
                     /locus_tag="VNW28_05485"
     CDS             337..1593
                     /locus_tag="VNW28_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HXA09412.1"
                     /translation="MLLTVSKRLEFSASRRLSLSGRSEAENLAAFGPETASRYGTGRN
                     YAAYFVFTGEVNATTGMLVNISEIKARVEEVLHARFDHKFLNEDHPVFRELLPTAENI
                     ARQLFVEVAPLFADLEARLVACHLVEAPERSATVYADRLSEENHWLEFSAARQTMSPH
                     LSATENDALFGTAAAPHGHGHHYRARFTFRHGPPNDAPGGTGAVPSPFRSGHVAPSPG
                     EIARCLASLAAELDHKNLNHDVAGLTNRPKTTESIAQYLHARVAAVLPVHRVRLHERE
                     DFFAEYWESGDYFLGMQRPFSAAHRLHAPGLSAAENFSLYGKCNNPRGHGHRYLTEAT
                     IGGRYDERSGTLYSFSAFRDGIERALRPWRDKHLDLETEEFREKPSTGENIVRALWPK
                     FNGQLNDRLIRLRLWETPNNRFTLRR"
     gene            1821..2138
                     /locus_tag="VNW28_05490"
     CDS             1821..2138
                     /locus_tag="VNW28_05490"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HXA09413.1"
                     /translation="MTAAQFRRLALGLPDVVESSHLAHPDFRVRNRIFATLGYPDPRY
                     GMVKLTPGQQEELLRAAPEIFGPAAGAWGRSGSTVVLLSAARVARLAPILRRAWENVA
                     ARK"
     gene            complement(2248..3816)
                     /locus_tag="VNW28_05495"
     CDS             complement(2248..3816)
                     /locus_tag="VNW28_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09414.1"
                     /translation="MSGRLTRLAAIVRVDFLVRFRRVSTVVVFLLLSAFAYVWIPSPA
                     TGRALIQINGQRAIYNSGAIGMGTASLGMIFVGLFGFYVISSAIGKDVVSRCGLVAAS
                     TPMRSGEYLFGKFLGNVLFLTTFLGGFMLSSMAMLLVRGEARIEPLVFLQQYLLLTPA
                     AVVFVSAVAILFESIRFLSGKLGDVIYFFLWMAGVGLVVSNEAGHHGINWARYFDFTG
                     FGFMIDQLTRTLHTDSVAIGASPFDPKKAPIVFSGLTLTREWLLPRLFSTLAPLLLLP
                     LAALCFHRFDPARTKQTAEKSQRNWIGRVQNLFKPASRRIVAQLLRPARRGSLVAAMW
                     SDAVLTLTLYPLVLIAFVVITIAATSAPLAGLMPGVFATLAIVVSDVATRDARANTIA
                     MLRATPRLRENFVWWKLGSTALLSLFLCAGPLARVATHGAQPLGALLVGIAFVAATAT
                     SLGVITVNAKTFIVVFLSFWYLVVNDRGANPWLDFAGFYGSGSTRTMLTYAVLSVVAL
                     VLAEGRHRMRLAAA"
     gene            complement(3813..4685)
                     /locus_tag="VNW28_05500"
     CDS             complement(3813..4685)
                     /locus_tag="VNW28_05500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA09415.1"
                     /translation="MLKIEAVSKQFRGGQFGVRDVSLDLKSGVIGLLGPNGAGKTTLM
                     QMIATITRPTSGRILFEGVDVTKRPNDVRRKLGYLPQDFGVYENLTAIEFLTYFAALK
                     GVNSKKRVMEMLENVNLHQSANRAVGGFSGGMKQRLGIAQALINDPALLIVDEPTAGL
                     DPEERVRFRNVLSEIGFGKLVILSTHIVSDIESIATEIAVLKEGALLTVGAPENLLQA
                     SAGRVWRSVLSSEEFDRLRTTLRISSAIRKADGVHIRFVGPEPAIAASSVEPELEDAF
                     LYLMNFGAGEETAR"
     gene            4905..5633
                     /locus_tag="VNW28_05505"
     CDS             4905..5633
                     /locus_tag="VNW28_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09416.1"
                     /translation="MSRLTKIGITVVAIVAVLPIVVIIVVVLFVCMPEFTPPQVARRE
                     IQLANHGSLIIDGKERGRSEHGFSQRAGYRPPGSAKIEWFGEVSDGVEPRVYQAGSLV
                     VVIDLPSAQLYIRTHAVHAATEDERDREGGGVNLHHIVRPRLEENWKSLALIFPNDIG
                     PFPISYYAEKNGLTTEEVSRINQLGGKRERKYPTTYIQSFDPETRDLKCSYQVDNKSS
                     WPLHLRLSADGTRLALIGIGGSSP"
     gene            5996..7942
                     /locus_tag="VNW28_05510"
     CDS             5996..7942
                     /locus_tag="VNW28_05510"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HXA09417.1"
                     /translation="MDEMAKIERAVAAGKLLPSSAANMRRTLESATSPIAVLSIAELV
                     EAGEWTELNDRFFRALAFGTGGIRGRTIGKIVTGAERGSPNDLGRPEFPCVGTNAMNY
                     DSVGRATQGLVEYLRAWFAQQKLSGRPKLVIAHDTRFFSADFTKLAAEIAAQNGCDAC
                     IFDGPRSTPELSFAVRYLGASAGVVVTASHNPPHDNGYKVYFRDGAQVVEPHASGIIA
                     RVNAAGAPVPNERPGAIVPLGREVDEAYMARLETLVLDPALVRAQRDLRIVYTPIHGT
                     GAVILRPMLERLGFTFDVVPEQDQFDGRFPTVQSPNPENAEALKLGVQLAQKRNADLV
                     IATDPDCDRMGVAVRSGNGEMKLLTGNQIGALIAYYRAKALFGQGVLTPQNAGRGVII
                     KTFVTTDLQKAIAEKFGLRCVETLTGFKYIGAKLEKYERAVPLPAGKDYRDLPEAETR
                     RLRLAHSAFYIFGGEESYGYSGADFVRDKDGNGATIMFCEVAAYAKSLGLTVDALLDQ
                     VYAQVGYYLEKNGALTFEGAEGAAKIQRLLATYASDPPNEMLGSKVTSIRNFETDTIR
                     DVEGDEIPKEKMLIFELADRTRVAVRGSGTEPKIKYYLFAQRRREGGEFTPAELEAIK
                     PQVEKKLEALWQWLQEDAASRTSG"
     gene            8169..8477
                     /locus_tag="VNW28_05515"
     CDS             8169..8477
                     /locus_tag="VNW28_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09418.1"
                     /translation="MSEIKLNGGEITILKTLGLSGGTMAGAQLLDRMEGLEGTEFLET
                     LTGLIALDYVVANKVNIRDMDSVKAASFRVNAAVARDLRDAVYPSRQVKPEGRRRRRS
                     "
     gene            8485..>9555
                     /locus_tag="VNW28_05520"
     CDS             8485..>9555
                     /locus_tag="VNW28_05520"
                     /inference="COORDINATES: protein motif:HMM:NF039484.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09419.1"
                     /translation="MSEESRIGKWLGFWPWLAAVLTGVLTALCFAPFEQTWICWIALT
                     PLLAAVWFSGEGSKRRWLRDLFLGYVAGVVFFWGVFYWLYTVTVPGLILVGLYMGVYF
                     GLWSWLAGLVRPGPRRAQEKQKAPNVQSASGGPASNVQLRNPLPNESPETRSPWLNSF
                     RNLGLALILAAGWTSLEWVRSWMFSGWGWNGLGVPLHDVLPIIQIAEFTGVAGLSFVV
                     AFANVIGVATVRRFTIESRVRVRRPHYDFTLTLAAIVGLCGYGIRLLQVPAETEELRV
                     AAVQANVPRDEKFGREFQAKTFAQFTRLTRLALATQPPPQLIIWPESSTPGPALLDEE
                     NYRFVMDLSASIKTDLLLGTIDA"
BASE COUNT         1847 a         2930 c         2969 g         1809 t
ORIGIN      
        1 gagcatcacc ttttgccctt catcggccga gcccacgtcg cttatgtgcc cgacggccgg
       61 gtgatcggcc tctcgaaggt cgcgcggatc gtggacgtgt tcgcgcgccg tttgcagatc
      121 caggaacaac tgactacgca ggtggccgac gcgctcatgc gctctcttca tccgttaggc
      181 gtggcggtcg tgatcgaggc gaagcatctc tgcatgatga tgcgcggggt ggagaaacaa
      241 aactcgatca tgaagacttc ctgtctcctc ggtgttttca aggaggacgc gcgcactcgc
      301 agcgagttcc tcgccctcct cggccagtaa cagccaatgc tgttaacggt cagcaagcga
      361 ctcgagtttt ccgcgtcgcg tcggctttct ctttccggtc ggtcggaagc ggaaaatttg
      421 gcggcgtttg gccctgaaac cgcctctcgc tacggcaccg gtcgcaacta cgcggcctat
      481 tttgtcttca ccggtgaggt taacgcaacg accgggatgc tggtcaacat cagcgaaatc
      541 aaagcccggg tggaggaagt cctgcatgcg cggtttgacc acaagttctt gaatgaagat
      601 cacccggtct tccgcgaact gctgccgact gcggagaaca tcgcgcgtca gctcttcgtc
      661 gaagtcgcgc cgctctttgc tgatctcgag gccaggctcg tcgcgtgtca tctggtcgag
      721 gcccccgagc ggagcgcgac cgtttacgcc gatcgattga gcgaggaaaa ccactggctc
      781 gaattttccg cggcccgcca gaccatgtcg ccgcatttaa gcgcgaccga gaatgacgcc
      841 ctctttggga cggcggcggc gccgcacggc cacggacatc attaccgtgc gcgttttacc
      901 tttcgccacg gtccgcctaa cgatgcgcct ggagggacgg gcgccgtccc gtccccgttt
      961 cgatccgggc acgtcgctcc atctcccggc gagatcgcac gatgcctcgc ctcgctcgcg
     1021 gcggaactcg atcacaagaa cctgaaccac gacgtcgctg gcctaacgaa caggccgaag
     1081 acgacggaaa gcatcgccca atacctgcac gcgcgggtcg ccgccgtgct gcccgtgcat
     1141 cgtgttcgcc tgcacgagcg cgaggatttt tttgccgaat attgggagag cggggattac
     1201 ttcctcggga tgcagcgtcc cttcagtgcg gcccatcgcc tgcatgcccc cggcctctcg
     1261 gcggcggaga atttttcgct ctacggcaaa tgcaacaacc cgcgtggcca tggtcaccgc
     1321 tacctcacag aggcgacaat cggcgggcgc tacgacgaac gcagcgggac gctttacagt
     1381 ttttccgcgt tccgcgacgg gatcgagcgg gcgctccgtc cgtggcggga taaacatctc
     1441 gatctcgaga cggaggaatt tcgagaaaaa ccctcgaccg gtgaaaacat cgtccgcgcc
     1501 ctctggccaa agtttaacgg ccagctcaat gatcggctca ttcgcctgcg gctctgggaa
     1561 acgccgaaca atcgcttcac gctgcgccgt taggcctgcg tcgcctcgcc gggcacggct
     1621 tgttccgctc cccggcttcg cctagccttg ctcatggcga aggtgggaac ggacaagcta
     1681 tctggtggaa cgtttcgcgg acaccgtctt tatcctggcc ttgcttttga tggtcgcggt
     1741 ctgcgcgttg gtgaccttcg ccatcgccgt caacacatga cttccaggcc gtgcggttct
     1801 ttattgtcag agggacgctt atgaccgccg cacaatttcg ccgcctcgcc ctcggcctgc
     1861 ctgacgtcgt ggaaagttcg cacctagcgc atccggattt ccgtgtccgg aacaggatct
     1921 tcgccaccct cggctatccc gatccacgct acgggatggt taagctgact cccggacaac
     1981 aggaagaact gctccgcgcc gcgccggaaa tatttggccc ggccgcgggc gcctggggcc
     2041 ggagtgggag cacggtggtg ctcctctccg ccgcccgggt ggccaggctc gctcccatcc
     2101 tccggcgtgc ctgggaaaac gtcgccgccc ggaagtagcc agcgaaccgc ccgcgatcca
     2161 atcctggagg gacgggctct gtcccgtccg tgtttctttg gtttgtcaga aacggggacg
     2221 acacagaggt cgtccctcca aacgtcgtta ggccgcggcc aatcgcatcc gatgccgccc
     2281 ctccgcgagg acgagcgcaa ccacgctgag gactgcgtag gtgagcatcg ttctcgtgct
     2341 cccgctcccg taaaagccgg cgaaatccag ccatggattc gctccgcgat cgttcaccac
     2401 cagataccag aaactgagga agacgacaat gaaggtcttg gcgttgaccg tgattacgcc
     2461 taacgaagtg gccgtcgccg cgacgaacgc gatcccgacg aggagcgcgc cgagcggctg
     2521 cgcgccgtgc gtcgccacgc gcgcgagcgg acccgcgcag agaaaaagac tgaggagcgc
     2581 ggtcgacccg agcttccacc aaacgaaatt ttcgcgcagg cgcggcgtgg cccgcagcat
     2641 cgcgattgtg ttcgcccgcg cgtcgcgcgt ggccacatcc gagacaacga tcgccagcgt
     2701 tgcgaagacg cccggcatca gacccgcgag aggtgcgctc gtggccgcga tcgtgatcac
     2761 gacgaatgcg ataagcacga gcggataaag tgtgagcgtg agcacggcgt cgctccacat
     2821 cgccgcgacg agcgaacccc ggcgcgccgg gcgcagcaat tgcgcgacga ttcgccggct
     2881 cgccggtttg aacaaattct gcactcgccc gatccaattg cgttgcgatt tctccgccgt
     2941 ctgcttcgtg cgcgccggat cgaaccgatg gaaacagagc gccgccagcg gcagaaggag
     3001 cagcggagcc agggtcgaaa agaggcgcgg cagcagccac tcgcgcgtca aagtcagccc
     3061 ggaaaaaacg atcggcgcct ttttcggatc gaagggcgac gcgccgatcg cgaccgagtc
     3121 ggtatgaagc gtgcgtgtca gctggtcgat catgaatcca aatccggtga aatcgaaata
     3181 gcgcgcccaa ttaatgccgt ggtggccggc ctcgttgctc acgaccagcc cgacgcccgc
     3241 catccaaagg aaaaaataga tgacgtcccc cagtttcccc gagaggaacc ggatcgactc
     3301 gaacaaaatc gccaccgccg agacgaagac gaccgccgcc ggcgtgagga gcagatattg
     3361 ctggaggaaa acgagcggct cgatccgtgc ctcgccgcgc acgagcagca tcgccatcga
     3421 gctgagcatg aatccgccga gaaacgtggt cagaaacaaa acgttgccga gaaatttccc
     3481 aaacagatat tcgcccgatc gcatcggggt ggaagcggcc accagtccgc agcgcgagac
     3541 gacatctttg ccgatcgcgc tcgagatcac atagaaaccg aagagcccga caaagatcat
     3601 cccgagggaa gcggtcccca tcccgatcgc gcccgagttg tagatcgcgc gctgtccatt
     3661 gatctggatg agggctcggc cggtcgcggg cgaggggatc cagacatagg caaacgcgct
     3721 caaaagcagg aaaacgacaa cggtcgagac gcgccggaag cgaacgagaa aatccacccg
     3781 cacgatggcg gcgaggcggg tcaggcggcc actcatcgcg ccgtctcctc gcctgccccg
     3841 aagttcatca aatagagaaa cgcgtcttcc aactccggtt ccacggacga cgcggcgatc
     3901 gccggctccg gcccaacgaa gcggatatga acgccgtcgg ctttgcggat cgcgctcgaa
     3961 attcttaggg tggtgcgcaa gcgatcgaat tcctccgaag aaagcacgct gcgccacacc
     4021 ctgcccgcgc tggcctggag aaggttctca ggcgcgccga cggtgaggag cgcgccttct
     4081 ttcaacactg cgatctcggt cgcaatcgat tcgatgtccg agacgatgtg ggtcgaaaga
     4141 atcaccagtt tgccgaagcc gatctcggaa agaacgttgc ggaatcggac ccgttcctcc
     4201 gggtcgagcc cggccgtcgg ttcatccacg atgaggagcg cgggatcgtt gatcagcgcc
     4261 tgtgcgatcc cgaggcgctg cttcatccca ccggaaaacc cgccgaccgc gcgattcgcg
     4321 gattgatgga ggttcacatt ttcgagcatt tccatcacgc gctttttcga gttcacgccc
     4381 ttgagcgcgg cgaagtaggt gaggaattcg atcgcggtca gattttcata aaccccgaag
     4441 tcctgcggca ggtagccgag ctttcgtcgc acatcgttag gccgcttcgt cacatcgacg
     4501 ccttcgaaca aaatccggcc gctcgtcggc cgcgtgatcg tcgcgatcat ctgcatgagc
     4561 gtcgtcttgc ccgcgccgtt cggaccgagc agcccgatca cgcccgattt caggtcgagc
     4621 gagacgtcgc gcacgccgaa ctgtccgccg cggaactgtt tcgagaccgc ctcaatcttc
     4681 agcatcgcac tttcttggcg ggaagagccc gtcctgtcga gccgcggtgc aaatttcggc
     4741 cgtgcgctcg gaggcgccgt ctcgtcgctg acccgcttgc gtttaccagc ctgaagtggt
     4801 tgccgacccg ccgtttagaa tgaggtgatt tccaaatgac cccattctgg taggacgaga
     4861 gctttcttgt gatggtacga agcttgcagc attcaaggca aaaaatgtct cgcctaacta
     4921 agattggtat aaccgtcgtc gcgattgtgg ccgtgctgcc gatcgtcgtc atcatcgtcg
     4981 tcgtgctgtt cgtctgcatg ccggagttca cgccgccgca ggtggctcgt cgggaaatac
     5041 agctcgcgaa tcacggcagt ctaatcatcg acggaaaaga gcgtggtcgg agcgaacacg
     5101 gtttttcgca gcgcgccggg taccgtcctc cgggatcggc caagatcgaa tggtttgggg
     5161 aagtgtccga tggagttgaa ccgcgggttt atcaggccgg atcgctggtt gtggtgattg
     5221 atcttccctc ggcgcaactc tacatccgga cacacgcagt ccatgccgca acggaggacg
     5281 agcgggatcg cgagggcggc ggtgtcaatc ttcatcatat cgttaggcct aggttggagg
     5341 agaactggaa gagtctcgcc ttgattttcc ctaatgatat tggcccgttc ccgatttcct
     5401 attacgcgga aaagaacggt ctgacgacag aggaagtctc gcggatcaac caactgggag
     5461 gcaaacgcga acggaagtat cccacgactt acatccaaag cttcgatcca gagacgcgcg
     5521 atttgaagtg ctcttaccag gtcgacaata agagttcatg gcctttgcat ctgcgactat
     5581 cggccgacgg gacacgtctt gccctgattg gaatcggcgg gtcatctccc tgacaccccc
     5641 tcatcgggtt aggcctatgg gcgttcaggg ggccttattg gcaggctggt ggttgccaaa
     5701 gctccacctt gcttccttcg gaatcggacc gctaagcaaa gcgccggagg ggtaattctc
     5761 tccggcgctt tttgcgtact cgatcacgtc ggcggcgtcc tggcggcttc gattacagac
     5821 ctggtgcaag cccaataaac tcctttggaa agggtgcgca atctggtgcg cttgctgggc
     5881 cgggggtcgc cgcactgagg cctttgccat gaccggcggt cgcggcactg aggcccctcc
     5941 cacgttaaga agtccgcggc ttgcaaagag cgggctgcgc cggaaaggtg gcgccatgga
     6001 cgaaatggcg aagatcgagc gggcggtggc ggcggggaag ctgctgccat ccagcgccgc
     6061 caacatgcgg cggacgctgg aaagcgcgac ctcgccgatt gcggtgttgt cgatcgcgga
     6121 actcgttgag gccggtgaat ggacggaact gaacgatcgt ttctttcgcg cgcttgcttt
     6181 cggcaccggt ggcattcgcg gccggacgat cggcaaaatt gtgaccgggg ctgagcgcgg
     6241 ctcgcctaac gatctcggcc ggccggaatt tccctgcgta ggtacgaacg cgatgaacta
     6301 cgacagcgtc ggacgcgcga cgcaggggct ggtcgaatat ttgcgcgcgt ggtttgcgca
     6361 gcaaaaactg agcggccggc cgaagctggt gatcgcgcac gacacgcgtt ttttctcggc
     6421 ggattttacg aaactcgcgg ccgagatcgc ggcgcagaat ggttgcgatg cctgtatctt
     6481 cgacggtccg cgctcgaccc ccgaactctc cttcgccgtg cgctacttgg gcgcgagcgc
     6541 gggtgtcgtg gttaccgcaa gccacaaccc gccgcacgac aacggctaca aagtttactt
     6601 ccgcgacggc gcgcaggtgg tcgagccgca cgccagcggc atcatcgccc gggtgaacgc
     6661 cgccggcgcg cccgtgccta acgaacgacc cggcgcgatt gtccctctcg ggcgcgaggt
     6721 cgacgaagcc tacatggcac ggctcgagac gctcgtgctc gacccggccc tggtccgggc
     6781 gcaacgcgat ctgcgcatcg tctacacccc gatccatggc acgggcgcgg tcatcctccg
     6841 tcccatgctc gagcggctcg ggttcacttt cgacgtcgtg ccggaacagg atcagttcga
     6901 cggtcgcttt ccgacggtgc aatcgcccaa cccggaaaac gccgaagcgc tcaaactcgg
     6961 cgtccagctg gcgcagaaac gcaacgccga tctcgtgatc gcgaccgatc cggattgcga
     7021 ccggatgggt gtcgcggtgc ggagcggaaa tggtgaaatg aagctgctga ccggcaacca
     7081 gatcggtgcc ctgatcgcct attaccgggc gaaggcgctt tttggtcaag gtgtgctcac
     7141 tccgcaaaac gccgggcgcg gggtgatcat caagactttc gttacgaccg atctgcagaa
     7201 agcgatcgcg gaaaaatttg gcctgcgctg tgtcgaaacg ttgaccggtt tcaaatacat
     7261 cggcgccaaa ctcgagaaat acgaacgcgc cgtgccgctg ccggctggca aagattatcg
     7321 cgatctgccg gaagcggaaa cgcgccggct gcggcttgcg cactcggctt tttacatttt
     7381 tggcggcgaa gaaagctacg gctacagcgg ggcggatttc gtgcgggaca aggacggcaa
     7441 cggcgcgacg atcatgttct gcgaagtcgc ggcctacgcg aaatcgctcg gcctgacggt
     7501 cgacgccttg ctcgaccagg tttacgcgca ggtcgggtac taccttgaga agaacggcgc
     7561 gctcactttc gagggcgcgg agggcgcggc caaaatccag cggctcctcg ccacctacgc
     7621 gagcgatcca cctaacgaaa tgctggggag caaggtgacg agtatccgga attttgagac
     7681 cgacacgatc cgcgatgtcg agggggatga aattccgaag gagaagatgc tcatcttcga
     7741 attggcggat cgcacgcgcg tggcggtccg cggttccggc acggagccca agatcaagta
     7801 ttatcttttc gcccagcgtc gccgggaagg tggggaattt acgccggcag agctcgaggc
     7861 gatcaaaccg caggtcgaga aaaagcttga ggcgctctgg cagtggctcc aggaagatgc
     7921 tgcgtcgcga acgtcgggct agcgattcca ccggtcgcgc tcgattgaga aaggacccgt
     7981 tcatcgtcat cccgagcgga gcgcagcgaa gtcgagggat cccgtggcga aggctttaag
     8041 gtaacaccgc gggatccctc gactgcgctc gggatgaccg cagggcccac caccccggag
     8101 tgcaatcaac cgatggcaaa ttttccatcc gccactcgac atccagccgg acgcagtgac
     8161 aattggtcgt gagcgaaatc aaactgaacg gcggcgagat tacgattctg aaaacgcttg
     8221 gcctgagcgg agggacaatg gcgggcgcgc agttgctcga tcggatggag ggattggagg
     8281 gtacggagtt tctcgaaacc ctcaccggct tgatcgcgct cgattacgtg gtggcgaaca
     8341 aggtcaacat tcgagacatg gactcggtca aggcggcctc ctttcgggtc aacgcggccg
     8401 tcgcccgtga tttgcgcgac gccgtttatc cgtcccgcca ggtcaagcca gaaggacgac
     8461 gccggcggcg gtcctgattt tcgagtgagc gaagagtcgc gcatcgggaa gtggctcggg
     8521 ttctggccat ggctggcggc ggtgcttacc ggcgtgctga cggcgctctg tttcgcgccg
     8581 tttgaacaga cctggatttg ctggatcgcg ctcacgcctc tgctcgcggc ggtctggttt
     8641 tccggtgaag gaagcaagcg ccgctggttg cgcgatctct tcctcggcta cgtcgcggga
     8701 gtcgtttttt tctggggcgt cttttactgg ctctacaccg tgaccgtgcc ggggctgatc
     8761 ctcgtcgggc tttacatggg ggtttatttt gggctttgga gctggctcgc aggactcgtt
     8821 aggccgggtc cgcgaagggc gcaggagaag cagaaagcgc ccaacgtcca atccgcctca
     8881 ggcggaccag cgtccaacgt tcaattgaga aatccgctgc ctaacgagtc gccggagacg
     8941 cgctcgccct ggctcaactc ctttcgcaac ctcgggctcg cgctcattct cgccgctggc
     9001 tggacgagcc tcgaatgggt gcggagctgg atgttcagtg gctggggttg gaacggcctc
     9061 ggtgtcccgt tgcacgacgt cttgccgatc atccagatcg cggagttcac cggtgtcgcc
     9121 gggctttcct tcgtggtcgc gtttgccaac gtcatcggag tcgctacggt gcggcgcttc
     9181 acgatcgaga gcagggtgcg cgtgcgacgg ccgcattatg attttactct caccctggcc
     9241 gcgatcgtcg gcctctgcgg ctacggcatc cggcttttgc aggtgccggc cgagacggaa
     9301 gagctgcgcg tagccgcggt ccaggccaat gtgccgcggg acgaaaaatt cggccgggag
     9361 tttcaggcga aaacgtttgc ccagtttacc cgactgacgc gccttgctct cgccactcag
     9421 ccgccgccgc agttgatcat ctggcccgaa agctccactc ccggaccggc gctgctcgac
     9481 gaagaaaatt accggtttgt gatggatctg tccgcttcga tcaagaccga cctgctcctg
     9541 ggcacgatcg acgcc
//
LOCUS       DATMNS010000158        18432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_572481, whole genome shotgun sequence.
ACCESSION   DATMNS010000158 DATMNS010000000
VERSION     DATMNS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 18432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18432
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572481"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VNW28_05525"
     CDS             complement(<1..388)
                     /locus_tag="VNW28_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA09420.1"
                     /translation="MPAIEASGLTKIYRTYRKESGLWGAVKGLVRRRHDETRAADGVS
                     FRIEEGEFVGFLGPNGAGKTTVLKMLSGLLNPTSGKATVLGFTPWERKDEMKRQFSLL
                     MGQKNALWWDLPARESLELNRAIYGIE"
     gene            complement(402..2294)
                     /gene="dxs"
                     /locus_tag="VNW28_05530"
     CDS             complement(402..2294)
                     /gene="dxs"
                     /locus_tag="VNW28_05530"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HXA09421.1"
                     /translation="MRLLTGIHSPADVKALPEESLPQLAQEVRDELIKVLSQTGGHLG
                     PNLGVVELTIALHRVFTTPHDRFVFDVSHQGYVHKMFTGRLDRIHTMRQYEGLNGFLL
                     RTESEHDSYGAGHAGTALSAGLGMAVGRDLLGGDENIVVVAGDAALTCGISYEALNNV
                     RAQTKRFIVVLNDNEWSIAKNVGAIAAYLNNIVTNPTYAHLHDKAGRFIKAIAGRGIL
                     HLAHKVEEGVKNLLVPSVIFEELGLRYYGPIDGHDIPLLTRTFEFLKTQDEPVLLHIL
                     TKKGKGYEPAIAKPDKFHGLGKFTAATGETATAATPTYSEILGKTLGKFADTNRKIVA
                     ITGAMPSGTGLGYFQAQHPDRYYDVGIAEEHAALFACGLATQGLKPFLTIYSTFMQRA
                     YDMIIHDIALQNLNVALCMDRAGLSGDDGPTHHGVFDIGYLRHIPNIVHMQPKDEDEF
                     VDMLWTMANYNSGPIAVRYPRGAGTGAKPKATPKLLEIGKAEVVQHGKDVAIFGLGNM
                     FELAQEAAQKLEALGVSVALINPRWIKPLDTGTLEFFARSVQVLCTIEDHVLHNGFGC
                     AVMEHLHTQLINTPVVRIGWPDQFIEHGTIPILRKKHGLTSDALVEKVTPFLKKLAEK
                     TRPTAA"
     gene            complement(2311..2622)
                     /gene="xseB"
                     /locus_tag="VNW28_05535"
     CDS             complement(2311..2622)
                     /gene="xseB"
                     /locus_tag="VNW28_05535"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002140.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXA09422.1"
                     /translation="MPNQVKKLAPAANFEQAMKRLEEIVEQMESGDLPLEDLIVRYEE
                     GMKLVKVCQERLASAEQRIEIITRDSAGKPVVQEFAPAASVAAQPEPKGEPKNVDVSL
                     F"
     gene            2740..3141
                     /locus_tag="VNW28_05540"
     CDS             2740..3141
                     /locus_tag="VNW28_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09423.1"
                     /translation="MTSLKRILLIDHQPHLTAVVGSALQATGQYLIRQEDHCRRALHT
                     ALHFQPDLILLDAGPEHLELDEIAQQIHANTALQNVPVVCLTTLSPDGQIGSVGFFGG
                     YSFVANSFQLDDMVNCIAEMLRDDLALGASA"
     gene            3241..4629
                     /gene="asnS"
                     /locus_tag="VNW28_05545"
     CDS             3241..4629
                     /gene="asnS"
                     /locus_tag="VNW28_05545"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003037.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HXA09424.1"
                     /translation="MLTPTSVRDALHSTEPIGRVLVQGWVRTRRDSKDFSFIELNDGS
                     SLRNLQIIAKNTLSNYAVVQHLITGASIVVRGALVASQGKGQSWEVVADAIEIVGASD
                     DSYPLQKKGHTPEFLREIAHLRPRSNLFGSVFRVRSRLAFAIHQFFQERGFVYVHTPI
                     ITGSDCEGAGELFRVTTIDPRNPPRLPSGEIDEAKDFFARRTYLTVSGQLEAEAFACA
                     LSKVYTFGPTFRAENSNTSRHANEFWMIEPEMAFCDLNGDMDLAEELVKHLVRDTREN
                     CPDELGLFAKFVDQELLARLEFVVDRPFQRISYTEAVELLRKSGEAFEFPVEYGLNLQ
                     SEHERWLTEKHFKCPITVFNYPKEIKPFYMRLNDDDRTVTAMDLLVPGIGEIVGGSQR
                     EERLDVLEANLGRHKMNLADYKWYLDLRRYGTVPHAGFGLGFERMLMFVTGVANIRDV
                     IPFARTPGSAEF"
     gene            complement(4669..5562)
                     /locus_tag="VNW28_05550"
     CDS             complement(4669..5562)
                     /locus_tag="VNW28_05550"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXA09425.1"
                     /translation="MDQRSSRVPVSVLVPIKNEAENLPRCLASIAWADEIFVVDSQST
                     DGSLRIAEELGARAVQFEFNGTWPKKKNWALENLPFQNEWVFILDADEVLPAGAEAEF
                     RAALANPGATAGFWINRRFMFMGRWLRHAYYPNWNLRLFRHSLGRYEKLTDAATASGD
                     NEVHEHVVVEGPTSRLRCELDHYAFPSVEVFVEKHNRYSNWEARVAVANQHGPSGVVL
                     QSARVAQRRKLKRLSQRLPFRPLLRFLYVYFWQKGFLDGREGYYFARMHGFYEFLSVA
                     KTYELKNRHPERRRADPGMKS"
     gene            complement(5588..6148)
                     /locus_tag="VNW28_05555"
     CDS             complement(5588..6148)
                     /locus_tag="VNW28_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HXA09426.1"
                     /translation="MPEKALAPVRVDQTSPSQSPWSGGDRILRLLWEFCWSIFCVWTP
                     KPANPWRLFWLGVFGAKIHGTPFVHQRARIAIPWKLTLHDRACLGDRANAYSLGEIEI
                     GARAVVAQEAYLNTGSHDFADPAMPLVVAKITIGEDAFVGARAFVMPGVTIGARAIVG
                     ACSVVTRDVPENVIAVGNPCRVLRPR"
     gene            complement(6157..6543)
                     /locus_tag="VNW28_05560"
     CDS             complement(6157..6543)
                     /locus_tag="VNW28_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09427.1"
                     /translation="MESVEEDRGWGELVQERGAASGLNNITLRICPFDFSRAGGFDTS
                     DYLAQVRGCTHDVVVVDGSDDLFLCRPICFRVAEEQVAPGGIIVVDDSWRYPDLRRTH
                     QAQRLRVFESVGPARFGVTSTDVYFY"
     gene            complement(6806..7912)
                     /locus_tag="VNW28_05565"
     CDS             complement(6806..7912)
                     /locus_tag="VNW28_05565"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXA09428.1"
                     /translation="MKITIVLGAFFPVPPLMGGAVEKVWFALGQEFVRQGHSVVQVSR
                     AHPALPVAETIAGVRHVRVRGSSQPRSIVWLKWLDLLYSIRAFFALPKADIVVTNTFW
                     LPILLRNSRRGKIYVHVARFPKGQMRFYRHADRLQTVSTSVAEAIRREVPTMENKISV
                     VPNPLPGSMFAKSPKSPTNRAKLLLYVGRIHPEKGVHLLIEALAVLAEDWKAAIVGSA
                     DSGLGGGGVDYLAQLKEAARKLRCEVSWQGPIFDEAALAEQYRSARLFVYASLAERGE
                     TFGLAPLESMANGCPVLVSNLACFRDFVRPNETGFVFDHRANSPVTALATKLAAVLAD
                     DATLVSVAAAGLRESKNFAVENVGRKYLNDFQLT"
     gene            complement(7909..9090)
                     /locus_tag="VNW28_05570"
     CDS             complement(7909..9090)
                     /locus_tag="VNW28_05570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA09429.1"
                     /translation="MRILNCIHSLNPSIGGPLESVRQFSLVLTRRGHSVEVVSLDTPA
                     APWVGEFPGTAHALGPGRGSYGYAPRFVPWLKERRAGYDAVVVHGIWQYHSFGAWRAL
                     HGTPIPYFVFPHGMLDPWFNHTYRLKHLKKLLYWPWAEYRVLRDATAVLFTGEEERRL
                     ARESFRLYRCREAVVNFGTAAPEVDLCRAQEAFWNAFPQLRAKKFLLFLGRLHEKKGC
                     EELIRAFAALHHSGPGDEDLHLVLAGPCANETYLRHLQRLTTELMRGENAPPVTFTGM
                     LTGNPKWGAFAASEAFVLPSHQENFGIAVVEALACGRPVLISNKVNIWREIVEDGCGY
                     ADEDDLAGTERLLARWLATSPTERGRMSDSARRCFAQRFEVNQAVDSFLHVLQSPPRA
                     A"
     gene            complement(9155..9820)
                     /locus_tag="VNW28_05575"
     CDS             complement(9155..9820)
                     /locus_tag="VNW28_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09430.1"
                     /translation="MRSVLMFLSFLLPWSLRRSFLEKLLGYSIHPTSHIGFAWILPRH
                     LILDEHSRIGHLTLCKSIDLLQLGPHAIIGQLNWITGFPSGASRHFAHQTQRRPELIV
                     EAHASITSRHLIDCTARVHIGAFATVGGFRSQLLTHSIDLAASRQAAEPIEIGEYCFV
                     GTNVVVLGGSVLPHHSVLGAQSLLNKKWDQPYQLYGGVPAKPLKEFSPEMEYFRRPRG
                     FVW"
     gene            complement(9817..10944)
                     /locus_tag="VNW28_05580"
     CDS             complement(9817..10944)
                     /locus_tag="VNW28_05580"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXA09431.1"
                     /translation="MVLLIGNYPADQQQSMQRFAMMMLRGLTATGVAAELIQPQPFFG
                     RIKFAGRFVAKWLGYLDKFIFFPRELKRKLSAGFDLVHICDHSNAIYCRHLQGHPVVV
                     TCHDLLAVRGALGEATDCPASATGKYLQRWIIAGLKKADLIVCTSQATLADAERLVAR
                     KNTTPKLAMITLGLSYPYRKLPAEEARARLAASSAIDPDIPFALHVGSNAPRKNRAGV
                     LRIFARCKEKWNGSLVFAGKPLTASLRSLGRELGVADRIVEAPETANELLEALYNRAT
                     AALFPSTCEGFGWPIAEAHACGCPVICTDLEPMIEVAGCAGLRHPLDDEAGFAADLLR
                     LTNPLERAGESAKALENAKRFSAGRMISEYGELYRALAPAS"
     gene            complement(10925..12400)
                     /locus_tag="VNW28_05585"
     CDS             complement(10925..12400)
                     /locus_tag="VNW28_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09432.1"
                     /translation="MRALADRQTPPAQENLPRIFDRSQTIRNIRLLIWLYLWLLIFEG
                     VFRKWIFPQFSTPLLIVRDPVVIFIYALALRARLFPQNVYVVSLEIIALSSWAAGIIV
                     LLPFFGLRTIVLVTGYGMRSNFLHLPLIFIIPAVFTLEDVKRVGWWTIVGMIPMALLM
                     ALQFVASPESLVNRTAGAGEGLQIQAGGGKIRPPGVFSFVSGTIYYLSAATAFLLHAV
                     LAKLPYRTWLLAASGAGLIVSLGVSGSRSTVLAVVLVVASVGAILVLRPNLVNKFGRH
                     LLLAVLLIWTISYLPIFQQGLGILSSRFTDSAEQGDTIVVADLIGRVLEGFTEALRFL
                     TRAPLGGYGLGVGTAGGASFLTGHPDFLLAENEWSRILLESGPILGLAFVVWRSVLAC
                     KLGLFAVRQLLAGNTLPLFLLSAGLFVFLQGPFGQPTSLGFAVVFAGLILAARPLEQE
                     AALPKVDLPEPPRRIRRRSKYAERLHGSTTRAHSAHGPLDR"
     gene            complement(12397..13629)
                     /locus_tag="VNW28_05590"
     CDS             complement(12397..13629)
                     /locus_tag="VNW28_05590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXA09433.1"
                     /translation="MILLSHPTGNENVRQVARALADAGLLEEFWTSINWDPNSPINRY
                     LPRGLRELFGRRAFPENVRERTRSRPFRESARLLLPPSSLLMRHEKGWLSVDAVLRAH
                     DCAVARRMEKGSFRAVYAYEDCAMETFAIAKALGIRTIYDLPIGYFRAAQQIFAEERE
                     REPDWASTLIGARDSGEKLARKEKELELADCVAVASTFTAQTLRGAPVQPRVEIIPYG
                     APPPVTGEIGLHTGKLRILFAGSLGQRKGLSYALRAIEIFGEGRCEFTLLGRKVGGVC
                     PALDHALRRHRWLPSLPHAEVLRTMRESDVVVFPSLFEGFGLVITEAMSQGTPVITTP
                     HTAGPDIILDGVDGFLVPIRSAEAIAEKLETLDRERELLRAMKVAAREKAASRTWEKY
                     RERLIALAREVVGIRIAI"
     gene            complement(13626..14621)
                     /locus_tag="VNW28_05595"
     CDS             complement(13626..14621)
                     /locus_tag="VNW28_05595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXA09434.1"
                     /translation="MKIVFSSYAYAPSVGGIESVSALLAHEFLSAGHEVRLLTETPSP
                     GLDAREFPVVRRPSLTHLRALLRWGDVVFQNNISLRSLLPALLMRKPALVVHQTWVRD
                     TGGEIDWSSRMKRAMLSRVHNVAISRAIARDLGIDCEIIGNPYNDAIFRNETGLRPHP
                     ILFVGRLVSDKGADILLQAVAELRDLTSGVTIIGSGPEEPVLRALAQELRLEVRFLGA
                     SSGSELAQVLNEHQLLVVPSRWAEPFGVVALEGIACGCAVIGSKDGGLPEAIGPCGST
                     FPNGDVPALAREIRSVLTDENLRTRWRDASPVHLRRHQAKTVAARYLQMLEELTA"
     gene            complement(14702..15814)
                     /locus_tag="VNW28_05600"
     CDS             complement(14702..15814)
                     /locus_tag="VNW28_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09435.1"
                     /translation="MSEAGTILQIVPHAPGGRDGVGDYARILAGCLKKSHDLETTFIS
                     AAPSASARTEDGYPLLSPLRTVADGRRLETYGGIVLHYVNYGFNRRGVPVWLPSVLRR
                     LQIRVRARLVTVFHELYASGSWRQSAFWLRPIQMRIARLIAETSDVAIVSSEVFREQL
                     TTLAPLKRPIVRPVFSNLGEPALSSAEIANRDPHRWIIFGGTELIERSLRSFLRAAVL
                     IAERYAPRELWVFGGAENSTIRRALQAEKTIRTHYQPDVDAEVASKIMAASAFGWIDY
                     FRRPDDPMPVVLKSGVFATYCAHGVIPVFPHGGSPIKVGHDVLPGPFFVAPNGQELPA
                     ETERASAAQEVYSWYGRNAASGPLAATVAAAIRDQA"
     gene            complement(15807..17015)
                     /locus_tag="VNW28_05605"
     CDS             complement(15807..17015)
                     /locus_tag="VNW28_05605"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA09436.1"
                     /translation="MRPSAGPLRILIVVNLPWDSRLGAARVWIELAKEWTRAGHAVEK
                     FCLTDAYPKPAGSSAASALRQARFPRRAAAYVRRNAERFDVIDCLIGVLPFRKNSLRF
                     RGLLVARSVGLYRLYDQFLRQSRILWPDQPRGRWFGRLFHRFLAWRSWKDSEKALRHS
                     DLLNLPNEDERLELARDPQVRVPSIVQPYGLNDRFLESLSTAAVPAEQRLSRQRICFI
                     GMWSLRKGSRDWPRLMAAIRQRHPAVEFLFLGTMFEESVVRSDLETAEANRIFCRPIF
                     EESDLPSLLADCAVALFPSYIEGFGLAVLEQLAAGLPTIAYDVPGPRQILDPQRVRFL
                     TPVGDTAALAARASEILALSAPEYEKLSAECMTIAAGYRWRDIAHSTIKQYRNALDSL
                     GQPLAGSARE"
     gene            complement(17012..17857)
                     /locus_tag="VNW28_05610"
     CDS             complement(17012..17857)
                     /locus_tag="VNW28_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09437.1"
                     /translation="MLNFGYHCARWAGGLRRTAYRALLPAIGQTTVCSPRSIPLDVYS
                     YSGEAMMPEQVRSIRSFLRHAGRPQSFTVVSDGTHSERSRELLSKIDPSVAIRRAEEY
                     RPSDLPEKFRRYVTDHPLGKQLGLIMSLPCSGPALYLDADILFFAGAAEIARASRGEN
                     APAFYLKDCLDYSVDSRILRGRDESSPPVNAGLLLLLRRLDWSLGIQRLIELEGAADF
                     FTTQTIVQLVMHANGALPFDEQKYVLRLDDQFVYADRYAGSHLVLRHYVNPVRHKFWN
                     AVSPR"
     gene            complement(17859..>18432)
                     /locus_tag="VNW28_05615"
     CDS             complement(17859..>18432)
                     /locus_tag="VNW28_05615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09438.1"
                     /translation="VFFCLQGQVTVFLITFFGRQAASVAEVGALGRLAVIFVVMGNLL
                     TNIFAPAFARCQEPRQLRWIYAGILGGVTGFSLLVLSGAAFLPNEFLFVLGNKYSHLQ
                     HELLLMVGGAILSMMASTVWTLNASRAWIAGSWLYIPLTLGTQLMLIPFTDFSSVSGV
                     LTFNLCSVFPSLALNLGLSYRGFRRPLPAG"
BASE COUNT         4029 a         5580 c         5469 g         3354 t
ORIGIN      
        1 gctcgatgcc gtagatcgcc cggtttaatt ccagcgactc ccgcgccggc aagtcccacc
       61 agagcgcgtt cttctggccc atcagcaggg agaactgccg cttcatctca tccttgcgct
      121 cccacggcgt gaagcccagc acggtcgcct tgccgctcgt cggattcagc aggccggaga
      181 gcattttcaa caccgtcgtc ttccccgcgc cgttggggcc aaggaacccg acgaactccc
      241 cctcctcgat gcggaagctc acaccgtccg ccgcgcgcgt ttcgtcgtgc cgacgcctca
      301 cgagaccctt gaccgcgccc cagagcccgc tctccttgcg ataggtccgg tagattttgg
      361 ttagcccgga ggcttcgatt gccggcacaa ccggggctga ttcaagcggc cgtcggccgc
      421 gtcttctccg ctaacttctt caggaacggc gtgaccttct cgacgagtgc gtcgctcgtc
      481 aggccatgct tcttccgcag gatcgggatg gtcccgtgct cgatgaattg atcgggccaa
      541 ccaatccgca cgaccggggt attgatcaac tgggtgtgca gatgctccat cacagcgcaa
      601 ccgaaaccgt tatgcagaac atggtcctcg atcgtgcaga gcacctgcac gctgcgggcg
      661 aaaaattcga gggtgccggt gtccagcggc ttgatccagc gcgggttgat cagcgccacc
      721 gaaacaccca gcgcctccaa cttctgggct gcttcctgcg ccagctcgaa catgttgccg
      781 aggccgaaga tcgcaacgtc cttgccgtgc tgcaccactt cggctttgcc gatctcgagc
      841 aatttcggag tcgccttcgg cttcgcgccg gtgcccgcgc cgcgcggata acgaaccgca
      901 atcggaccgg aattgtagtt cgccattgtc caaagcatgt ccacaaattc gtcctcatcc
      961 ttcggctgca tgtggacgat gttcgggatg tgacggagat agccgatgtc aaaaacgcca
     1021 tggtgcgtcg ggccgtcgtc tccactcaag ccggcgcggt ccatgcagag tgcgacgttc
     1081 agattttgca gcgcgatgtc gtgaatgatc atgtcgtagg cgcgttgcat aaaggtggaa
     1141 tagatggtga gaaacggttt cagtccctgg gtcgcaaggc cgcaggcaaa gagcgcggcg
     1201 tgctcctccg cgatcccgac gtcgtagtag cggtcagggt gctgcgcctg gaaataaccg
     1261 agacccgtcc cgctcggcat cgcgccggtg atggccacga tcttgcgatt ggtgtcggcg
     1321 aacttgccta acgtcttgcc cagaatttcc gaataggtcg gggtcgccgc cgtcgcggtc
     1381 tccccggtcg cggcggtgaa ttttccgagt ccgtggaatt tatccggctt ggcgatggcc
     1441 ggctcatagc ctttgccctt tttcgtcagg atatggagca ggaccggctc gtcctgggtc
     1501 ttcagaaact cgaaggttcg ggtgagcagg gggatgtcgt gaccatcgat cgggccatag
     1561 taacggagac cgagttcctc aaagatcacg ctcggcacga gcagattctt gacgccttcc
     1621 tcgaccttgt gcgccaggtg gaggatcccc cggccggcga tcgccttgat aaaacgtccg
     1681 gctttatcgt gcagatgcgc ataggtcggg ttggtcacga tgttgttcag gtaagcggcg
     1741 atcgcgccga cgtttttcgc gatcgaccac tcattgtcgt tcagcaccac gatgaaacgt
     1801 ttcgtctgcg cccggacatt gttcagcgcc tcatagctga tgccgcaggt aagcgcagcg
     1861 tcaccggcca cgaccacgat gttctcatcc ccgcctaaca aatcgcgccc caccgccatg
     1921 cccaggccgg ccgacaaggc ggtgccggcg tgacctgcgc cataggaatc gtgctcgctc
     1981 tcagtgcgaa ggagaaaacc gttgagtcct tcgtattgcc gcatcgtgtg aatgcgatcg
     2041 agccggccgg taaacatttt gtggacatag ccttgatgac taacgtcgaa gacgaaccga
     2101 tcgtgcgggg tggtgaaaac ccggtggagt gcgatggtca actcgaccac gccgagattc
     2161 ggcccgagat ggccgccggt ttgggagagg accttgatca gttcatcgcg cacttcctgc
     2221 gcaagctggg ggagactctc ctccggcaat gctttcacgt cggccggaga atgaatcccg
     2281 gtcaacaaac gcaccgtttc ggcaggtgcc tcagaagaga ctgacgtcga cattttttgg
     2341 ttctcctttt ggttcaggct gggccgcgac ggacgcggct ggtgcaaatt cttgtacgac
     2401 tggcttcccg gcgctgtcgc gggtaatgat ttcgattcgt tgttcagccg aggctaggcg
     2461 ctcctgacag accttgacca gcttcatccc ttcctcgtag cggacgatca aatcctcgag
     2521 cggaaggtca ccggattcca tctgttctac gatttcctcg agccgtttca tggcctgctc
     2581 gaagttcgct gcgggagcga gcttcttcac ttggttcggc ataacggcaa gagagcaatt
     2641 aagcatacag ggaccaatta aaaagtcgga cttggaggcc ccgaggagtg gcacagcgga
     2701 tgctataatt gccataactg agcctcgtta gacgatccga tgacttctct aaagcgcatc
     2761 cttctgatcg accaccagcc gcatctgact gcggtcgtgg gatcggccct tcaggcgacc
     2821 ggtcaatatt tgatccggca ggaagaccat tgccggcgcg cgctgcacac ggcgcttcat
     2881 ttccagccgg acctgattct gctcgacgcc gggcccgaac acctcgagct cgatgagatc
     2941 gcacaacaga ttcacgcgaa tacggcgctc cagaacgtgc cggtcgtttg cctaacgact
     3001 ctctcgccgg atggccagat cggatcggtc ggatttttcg gcggctacag cttcgtggcc
     3061 aattcgttcc agctcgatga catggtgaat tgcatcgcgg aaatgctgcg tgacgacctg
     3121 gcactcggcg cgtctgcctg acgcgccgcg gtttgcaccg agaatcggca gcgacccgcc
     3181 cccgagcact tgtcgatcct agcccttgta ggatcgcgat cgtccggcaa acttcctccc
     3241 atgcttacgc ccacttccgt cagggacgct ttgcacagca ccgagccgat cgggagagtg
     3301 ctggtgcagg gatgggtccg cactcggcgc gattcgaagg atttttcctt catcgaactg
     3361 aacgatggct ccagcctgcg gaacctgcag atcatcgcga aaaatacgct ctcgaattac
     3421 gccgtggtgc agcatctcat cacaggcgct tccatcgtgg tgcgcggtgc gctcgtcgca
     3481 tcgcaaggca aaggacaaag ctgggaggtc gtcgccgatg ccatcgagat cgtgggcgcg
     3541 tccgatgata gttacccgtt gcagaaaaag ggacacaccc cggagttcct gcgcgagatc
     3601 gctcatttgc ggccgcgctc caaccttttc ggcagcgtct ttcgcgtccg gagccggctc
     3661 gcttttgcga tccaccaatt ctttcaggaa cgcggttttg tttacgtgca cacgccaatc
     3721 attacgggca gcgactgcga aggcgccggg gagttgtttc gggtcacgac gatcgaccca
     3781 agaaacccgc ctcgtttgcc cagtggggag atcgacgagg ccaaggattt tttcgcgcgc
     3841 cggacttatc tgacggtcag cggacagctc gaggccgagg ctttcgcctg cgcgctctcc
     3901 aaggtctaca cctttggccc cacatttcgg gcggagaatt ccaacacctc gcggcacgcg
     3961 aacgagttct ggatgatcga accggagatg gccttctgcg acctgaacgg cgatatggat
     4021 ctcgcggagg agctggtaaa acatctcgtg cgcgacaccc gcgaaaattg ccccgatgag
     4081 ctcggtcttt tcgccaaatt tgtcgatcaa gaattgctcg cgcgcctcga atttgtcgtc
     4141 gaccgaccat ttcagcggat cagctacacc gaagcggtcg aattgctgcg gaagagtgga
     4201 gaagcgttcg aattcccggt cgaatacggc ctgaacctgc aatcggaaca cgagcgctgg
     4261 ctgaccgaga agcatttcaa gtgtccgatc accgtcttca attacccgaa ggaaattaag
     4321 ccgttttaca tgcgtctaaa cgacgacgac cgcacagtaa cggcgatgga cttgctcgtg
     4381 ccggggatcg gcgaaattgt cggaggcagt cagcgcgagg aacggctcga cgtcctggaa
     4441 gcgaacctcg gccgccacaa aatgaatttg gccgactaca aatggtacct cgatctccga
     4501 cgctatggaa cggtgccgca cgccggtttc ggccttgggt tcgagcgcat gctcatgttc
     4561 gtcactggag tggcgaacat tcgcgacgtg attccgttcg cccgcacgcc ggggagcgcg
     4621 gaattttagt cattccgggc gaagccgagg gacgccgctg cgttaccctt aagatttcat
     4681 cccgggatcc gctcggcgcc gctcgggatg acggttcttc agctcgtaag tcttggccac
     4741 cgacaggaac tcgtagaagc cgtgcatccg cgcgaaataa taaccctcgc gcccgtcgag
     4801 aaatcctttt tgccaaaagt aaacatagag gaaacgcagg agcgggcgaa acggcagccg
     4861 ttgggaaagc cgcttcagct ttcgccgttg cgcgactctc gcgctctgta acacgacgcc
     4921 gctcggcccg tgctgattgg ccaccgcgac ccgcgcttcc cagttggaat agcgattgtg
     4981 tttctcgaca aaaacctcga ccgacggaaa ggcgtagtga tcgagttcgc agcgcaatcg
     5041 actcgtcggt ccttccacga cgacatgctc gtgcacttcg ttgtcgccgc tggccgtcgc
     5101 cgcatcggtc agcttctcgt agcggcctaa cgaatgacgg aagagccgca gattccaatt
     5161 cgggtagtag gcgtgccgca gccagcggcc catgaacatg aagcgccgat tgatccagaa
     5221 tcccgccgtc gcccccggat ttgcaagtgc agcccgaaac tcggcctccg cgcccgccgg
     5281 cagaacttcg tcggcatcga gaatgaagac ccactcgttc tggaagggca gattttcgag
     5341 cgcccagttt tttttcttcg gccaggtgcc attaaattcg aactggacgg cccgcgcgcc
     5401 aagctcctcg gcgatgcgca gcgatccgtc cgtgctctgg gaatcgacca cgaaaatttc
     5461 atctgcccac gcgatcgagg caaggcaacg cggaagattt tcggcctcgt tcttgatcgg
     5521 cacaagaacc gagacgggca cgcggcttga tcgctgatcc atggcggaag ttgcggccac
     5581 gacttcttta ccgcggccgc agcacgcgac aaggatttcc caccgcgatc acgttctccg
     5641 gcacgtcgcg ggtcacgacg gaacacgccc cgacgatggc ccgcgcgccg atcgtcactc
     5701 ccggcatgac gaaggcccgc gcgccgacaa acgcgtcttc gccgatcgtg atcttggcga
     5761 cgaccagcgg catcgccggg tccgcgaaat cgtggctgcc ggtattcaag tacgcttcct
     5821 gcgcgaccac ggcgcgcgcc ccaatctcaa tttcgccgag actgtaggcg ttcgcgcgat
     5881 cgccgaggca ggcgcgatcg tgcaacgtca atttccaggg aatcgcaatc cgcgcacgtt
     5941 gatgaacaaa gggcgtcccg tgaattttgg cgccgaacac gcctaaccaa aaaaggcgcc
     6001 acggattcgc cggcttcggt gtccagacgc aaaagatgct ccagcaaaat tcccacaaaa
     6061 ggcgcaagat gcgatcaccg ccgctccagg gtgattggga aggagaagtt tgatcgaccc
     6121 ggactggagc gagcgctttt tcaggcatgg caagcttcaa taaaaataaa catcggtgct
     6181 tgttacaccg aagcgcgcgg gcccaacgct ttcaaaaaca cgaagccgtt gcgcctggtg
     6241 tgtccgacgc aaatcagggt accgccagga atcatccacc acgataatcc caccaggcgc
     6301 gacttgctct tccgccactc gaaaacaaat ggggcgacac aaaaacagat catcggatcc
     6361 atccacgacc acgacatcat gcgtgcagcc gcggacttgc gcgagataat ccgaagtatc
     6421 aaaaccgcca gcgcgcgaga aatcaaaagg acagatgcgc agggtgatgt tgttcagtcc
     6481 agatgctgcg ccgcgctcct ggacaagctc cccccagcct cggtcttctt ctacactttc
     6541 cacgctccgg caacgctgcg cgaagaaaag agtcgagccg ccactgccaa attcgaaaac
     6601 tctcatttcc ggccgcagga aatccttgag aaagtcgatc gcacgataag aaaaccatgg
     6661 taactccagt tcgatgggcg atcgcgaagc caaacagttg tgcaacacgt aacgcggaag
     6721 cagaccgggc tgccacatca agttgccgat cactttttgc acccgttgtc gattcgttag
     6781 ccgcatggct tagagaccga cgttttcaag tgagctgaaa atcattgaga tactttcggc
     6841 cgacgttttc gaccgcaaaa tttttcgact cgcggagccc ggccgccgct actgacacaa
     6901 gagtcgcatc gtcagcgagc accgccgcaa gttttgttgc cagcgccgtg accggagagt
     6961 ttgcgcgatg atcaaagacg aatcccgtct catttggcct aacgaaatcg cggaaacaag
     7021 ccagatttga gaccaggacc gggcaaccat ttgccatcga ttccaaggga gcgagaccaa
     7081 acgtttcacc gcgttcggca agcgacgcgt aaacaaatag ccgcgccgaa cggtattgct
     7141 ccgcgagcgc ggcttcatcg aagatcggcc cctgccaact aacttcgcac cggagcttcc
     7201 tggcggcttc cttcagctga gccaagtagt caacgccgcc gccgccgagc ccgctatcgg
     7261 ccgaaccgac gatggccgct ttccagtctt cagccagcac cgcgagagct tcgatcaata
     7321 gatgaacgcc tttttccgga tggatgcggc ccacgtaaag caataatttc gcgcgattgg
     7381 tcggcgattt tggcgatttc gcaaacattg agccgggaag tggattcggg acgacggaga
     7441 tcttgttttc catcgtcggc acttcgcggc gaatcgcctc agccaccgaa gtcgaaacgg
     7501 tctgcagccg gtcggcgtgc cgataaaacc gcatctgccc tttcggaaaa cgggcgacgt
     7561 gcacgtaaat ttttccgcgg cgcgaatttc gcagaaggat tggcagccaa aaggtgttag
     7621 tgacgacgat gtccgctttc ggcagcgcga aaaatgcgcg aatcgagtag agcagatcga
     7681 gccacttcaa ccaaacaatc gatcgcggct gggaagagcc acggacgcgc acatgccgaa
     7741 ccccggcgat ggtctccgca accggcagcg cgggatgggc gcgggaaacc tgcaccacgc
     7801 tgtgtccctg cctaacgaac tcctggccga gcgcgaacca gaccttttcg acggcacctc
     7861 ccatgagcgg gggaacgggg aagaacgcac cgagcacaat cgtaattttc atgcggcgcg
     7921 gggcggactt tgcaagacgt ggagaaacga atccaccgcc tgattgacct caaagcgctg
     7981 ggcgaaacag cggcgggcgc tgtcactcat ccggccccgc tccgtgggcg aggtggcgag
     8041 ccagcgggcg agcaggcgct cggtgccggc gaggtcatct tcatcggcgt aaccgcagcc
     8101 gtcctcgacg atctcgcgcc agatgttcac tttgtttgaa atcagaaccg gtcttccaca
     8161 ggcgagcgct tcgacgacgg cgatgccaaa attttcctga tgagagggca aaacgaaggc
     8221 ttcggacgcg gcgaaggcgc cccattttgg atttccggtg agcatgccgg tgaaggtcac
     8281 cggcggggca ttttctccgc gcatgagttc ggtcgttagg cgctgaaggt gccgcaggta
     8341 agtttcgttg gcgcacggtc cagcgaggac cagatgcaga tcctcgtctc cgggcccaga
     8401 atggtgcaag gccgcgaacg cccggatcag ttcctcgcag ccttttttct catgcaggcg
     8461 gccaaggaaa aggagaaatt tcttcgcgcg gagctgcggg aaggcgttcc agaaagcttc
     8521 ctgcgcccgg cacaaatcga cttccggggc ggcggtgcca aaattcacga ccgcttcccg
     8581 gcagcgatag agccggaagg attcccgcgc caggcgccgt tcttcctcgc cggtaaaaag
     8641 gacggcggtg gcgtcgcgca aaacgcggta ttccgcccac ggccaataga gcaacttctt
     8701 cagatgcttc agccgatagg tgtggttgaa ccacggatcg agcatcccat gcgggaagac
     8761 gaaatagggg atcggggttc catgcagcgc gcgccaggcg ccgaaactgt ggtattgcca
     8821 gatcccatgg acgaccacgg cgtcgtagcc ggcgcgccgc tccttcaacc acggaacgaa
     8881 acgcggcgcg tagccgtagc tgccgcgacc cggcccgagg gcgtgggccg tgccgggaaa
     8941 ttcgcccacc cacggagccg ccggtgtatc gagcgaaacc acctcgaccg aatgaccccg
     9001 ccgggtgaga acgagcgaga attgcctgac cgactcgagg ggcccgccga tcgacggatt
     9061 cagcgaatga atgcagttta gtattctcat ccggacgacg ctgcgactcg atcctccaag
     9121 atcgtcttct atctcgagcg cgctgcagca tcgctcacca cacaaatcct ctcgggcgcc
     9181 ggaagtattc catttcgggt gagaattcct tcagcggttt ggccggcacg ccgccgtaaa
     9241 gctggtaggg ctggtcccat ttcttgttta aaagcgactg cgcgcccagg accgaatgat
     9301 gcggcagaac gctgcccccg agcacgacaa cattggtccc aacgaagcag tattcaccga
     9361 tctcgatcgg ttccgccgcc tgccgactcg cggcgagatc gatgctgtgg gtgaggagtt
     9421 gcgagcggaa accgccgacg gtggcgaaag cgccgatgtg cacccgcgcc gtgcaatcga
     9481 tcaagtgccg gctcgtgatg ctggcgtggg cttcgacgat cagttccggg cgccgctggg
     9541 tctgatgagc aaagtgccgg ctggcgccgc tgggaaatcc ggtaatccaa tttaattgcc
     9601 cgatgatcgc gtgcgggccg agctgcaaaa gatcgatgct tttgcaaagc gtcaaatgcc
     9661 cgatgcgaga atgctcgtcc aggatgagat gtcgcgggag aatccaggcg aaaccaatgt
     9721 ggctggtggg atgaatggaa tagccgagca gtttttcgag gaacgagcgc cgtaacgacc
     9781 acgggagcag gaacgaaaga aacattagga cgctcctcat gatgctggcg cgagggcgcg
     9841 gtagagttcg ccatattccg agatcatgcg cccggcggaa aatcgcttcg cgttttcgag
     9901 cgccttcgcg ctctctcctg cgcgctccaa cggattcgtc aggcgcaaaa gatcagcggc
     9961 aaatccggct tcgtcgtcga gcgggtgccg aagaccagca caaccggcca cctcgatcat
    10021 cggctcgaga tcggtgcaaa tgacgggaca cccacaggca tgcgcctctg ctatgggcca
    10081 cccgaaacct tcgcaggtcg aagggaaaag cgccgccgtg gcgcgattgt aaagggcttc
    10141 gagcaattcg ttcgctgtct cgggcgcttc gacgatccgg tcggcgaccc ccagctcgcg
    10201 gcctaacgaa cggagggatg ctgtgagcgg tttgcccgcg aagacgagcg atccattcca
    10261 tttctctttg caccgagcga atatccgcag gacgccggcg cgattcttgc ggggcgcgtt
    10321 cgagccgaca tggagcgcaa atggaatgtc cggatcgatg gcggacgacg ctgcgagccg
    10381 cgcacgcgct tcttctgccg gcaatttgcg atagggataa ctcaaaccca gcgtaatcat
    10441 tgcgagcttc ggcgttgtgt tcttacgtgc caccaggcgc tccgcgtccg ccagcgtggc
    10501 ttgcgaggtg caaacgatca ggtccgcctt cttcagcccg gcgatgatcc aacgctgcaa
    10561 gtattttccg gtcgcggaag cgggacaatc ggtcgcctcc ccgagagcgc cgcggacagc
    10621 gagcagatcg tggcaagtga cgaccacggg atgaccctgg agatggcggc aatagatcgc
    10681 atttgagtga tcacagatgt ggacgagatc gaatcccgcg gagagcttcc gtttcagctc
    10741 tcgtgggaaa aaaatgaatt tgtcgagata accgagccac ttcgccacaa agcgaccggc
    10801 gaatttgatc cgaccgaaga atggttgcgg ctggatcaat tcggccgcca ctccggtcgc
    10861 ggtgaggccg cggagcatca tcatcgcgaa acgttgcatg ctttgctgct gatcagccgg
    10921 gtaattaccg atcaagagga ccatgggcag aatgagcgcg agtcgtcgaa ccgtgcaacc
    10981 gctcggcata tttggagcgt cgccggatac ggcgcggcgg ttccggcaag tcgacctttg
    11041 gaagagccgc ttcttgttcg agcggtctcg cagccaaaat taagccagcg aaaacgaccg
    11101 cgaatccaag actggtcggt tggccaaacg ggccttgcaa gaaaacgaag agaccggcgc
    11161 taaggagaaa aagcggcagc gtattccccg ctaggagctg acggacggca aagaggccga
    11221 gcttgcacgc gagcacggag cgccagacca cgaaggccag gccgagaatt ggtccgctct
    11281 cgagtagaat ccgcgaccac tcattttccg ccaacaagaa gtccgggtgg cccgtcagaa
    11341 agcttgcgcc cccggcggtg ccgacgccga gtccgtatcc gcccaaggga gctcgagtca
    11401 aaaacctcag cgcttcggta aatccttcga gcactctgcc aatcaaatca gccacgacaa
    11461 tcgtatctcc ttgttcggct gaatctgtaa atcggctgga aagaatgccg agtccttgtt
    11521 gaaaaatcgg aagatagctg atcgtccaaa tcaagagcac agcgaggagc agatggcgtc
    11581 cgaatttatt tacgaggttc ggacgaagaa ccaggatcgc accgaccgaa gcgacgacga
    11641 ggacgaccgc gaggaccgtc gaacgactgc ccgagacacc caggctaacg atcaaccccg
    11701 cgcccgacgc cgccagtagc caggtcctgt agggaagctt cgccaggacc gcgtggagaa
    11761 gaaaggccgt tgccgcgctc agataataga tggtgcccga gacaaaggaa aaaacgcccg
    11821 ggggccgaat cttgccgcca cctgcctgga tctgcaggcc ctcgccggcg ccagcggtgc
    11881 gattaacaag cgactcggga ctggccacaa actggagggc catcaacagg gccattggaa
    11941 tcatcccaac gattgtccac cagcccaccc gcttgacgtc ttccagagta aagaccgccg
    12001 ggatgataaa gatgagtggg agatgaagaa aattggaacg cattccgtat ccggtcacga
    12061 ggacgatggt acgcagcccg aagaaaggca ggagcacaat aatcccggcc gcccaggacg
    12121 agagggcgat gatttccagc gagacgacat agacattctg cgggaagaga cgggcacgca
    12181 gagccagcgc ataaatgaaa atgacgacag gatcgcggac gattaagagg ggagtggaga
    12241 actgaggaaa gatccattta cgaaagacac cctcaaagat gagcaaccaa aggtagagcc
    12301 agatcaagag ccggatattc cgaatcgtct gcgaccgatc gaagatccgc ggaagattct
    12361 cctgagcggg cggggtctgt cgatcggcga gggctctcat atcgcgatgc gtatacccac
    12421 cacttcccgt gccagcgcga tgaggcgctc ccggtatttt tcccatgtcc gcgaggctgc
    12481 cttctcccgg gcggcaacct tcatcgcccg gagcagttcc cgctcgcgat cgagcgtttc
    12541 cagcttctca gcgatcgcct ccgccgaccg gatgggaacg aggaacccgt cgaccccatc
    12601 caggataata tccggaccgg ccgtgtgcgg ggtcgtgatc accggtgtgc cttgcgacat
    12661 cgcttccgtg atgacaaggc cgaagccctc gaaaagcgat ggaaaaacga cgacgtcgct
    12721 ttcgcgcatc gtccggagga cctcggcatg gggtaacgat ggcagccagc ggtgacggcg
    12781 gagtgcgtgg tctaaggcgg ggcaaacacc gccgaccttg cggccaagga gcgtgaattc
    12841 gcagcgccct tccccaaaaa tctcgatcgc acgcaaagca taggacagtc ctttgcgctg
    12901 cccaagcgat ccggcaaaga gaatgcgcaa cttgcccgta tgcaggccga tttctccggt
    12961 caccggcggg ggcgcaccgt acgggattat ttcgacgcga ggttggaccg gcgctccccg
    13021 gagcgtttgc gcggtgaatg tgctggcgac cgccacgcaa tccgccagtt ccagttcctt
    13081 ctcttttcgc gccagcttct cgccactgtc ccgcgccccg atcaaggtcg aggcccagtc
    13141 cggttcgcgt tcgcgttcct ccgcaaagat ttgctgcgcc gcgcgaaaat agccgatcgg
    13201 caaatcgtaa attgtgcgga tccccagcgc cttcgcgatc gcaaacgtct ccatggcgca
    13261 gtcctcgtag gcataaaccg cacggaaaga tcccttctcc atgcgacgcg caaccgcgca
    13321 atcatgcgcg cgcagcaccg cgtcgacgct cagccagcct ttctcatgtc gcatcaggag
    13381 cgagctcggc ggcagcagaa gtcgcgcgct ctcgcgaaat ggacgggagc gcgttcgctc
    13441 gcgcacattc tcgggaaagg cacggcggcc gaataactcg cgcagtcctc gcggcaaata
    13501 tcgattgatc ggcgaattcg gatcccaatt gattgacgtc cagaattctt ccagcaatcc
    13561 agcgtcggcg agtgcgcgcg ccacctggcg cacgttctca tttcccgttg gatgcgagag
    13621 taggatcatg ccgtcagctc ctctaacatc tgcaggtagc gcgccgcgac cgttttcgcc
    13681 tgatgccgcc gcaaatgaac cggactggca tctcgccagc gggtgcgcag gttctcatcc
    13741 gtcagcacgc tacgaatctc ccgcgcaaga gccgggacgt cgccgttagg aaaggtactt
    13801 ccgcagggac cgatcgcttc gggcaggccg ccatccttcg acccgatcac tgcgcagcca
    13861 caggcgattc cctccagcgc gaccacgccg aacggctcgg cccaacgcga cggcacgacg
    13921 aggagttgat gctcattcaa gacctgcgcg agctccgacc cggaacttgc acccagaaag
    13981 cgaacttcga gacgcagctc ttgcgcgagt gcgcgcagca ccggttcttc cggcccgctc
    14041 ccgataatgg tgacgcctga tgtcagatca cgaagctcag ccaccgcttg gagcaaaatg
    14101 tcggcgccct tatcggaaac caggcgcccg acgaacagga tcggatgagg acgtagcccg
    14161 gtctcgtttc gaaaaatcgc gtcattgtag ggattgccaa taatctcgca atcgatcccg
    14221 agatcgcgcg caatggcgcg gctgatcgcg acattgtgaa cccgcgagag cattgctcgt
    14281 ttcatccgac tgctccaatc aatctcgccg ccggtatcac gaacccaggt ttgatgaacg
    14341 acaagcgcag gtttccgcat caacaaggcg ggcagaaggg aacgaagcga gatgttgttt
    14401 tggaagacaa cgtctcccca acgcaaaaga gcccgcaggt gagtgagcga tggccggcgc
    14461 acaaccggaa attcccttgc gtccagtccg ggcgaaggcg tttcggtgag gagtcgaacc
    14521 tcgtgacctg cgctcaggaa ctcgtgcgcc agcaaagccg aaacgctctc gattccgccc
    14581 acactcggcg cgtatgcata ggaagagaaa acgatcttca tcaacgctga acgctttgcg
    14641 cccccaggaa tattcttagc gggcgaccgt gatcgaacat gatttccggc atggctggaa
    14701 gttacgcttg atcgcgaatc gcggcggcga cagtggcggc gagtgggccg gaagcagcgt
    14761 ttcttccata ccatgaatag acctcctggg ccgcgctcgc gcgttccgtt tcggccggga
    14821 gttcctgccc atttggggcg acgaaaaagg gaccgggcag cacatcgtgg cccaccttga
    14881 taggcgagcc gccatgggga aaaaccggga tcaccccatg cgcacagtag gtagcgaaca
    14941 cgccggattt tagaacgacc ggcatcgggt cgtccgggcg ccggaaataa tcgatccacc
    15001 cgaaggcgga ggcggccatg atctttgagg cgacctctgc atccacatcc ggctgataat
    15061 gggtgcggat cgtcttttcg gcctgcagcg cccggcggat cgtggagttc tctgcgccac
    15121 caaacaccca cagctcgcgc ggcgcgtatc gctcggcgat caaaaccgcc gcccgcagga
    15181 aggagcgaag cgaacgctca atcaattcgg taccgccaaa aatgatccag cgatgagggt
    15241 cgcggttcgc gatttccgcg gaggagagcg cgggttcgcc caagttggaa aaaaccgggc
    15301 gcacgatagg acgctttagg ggcgccaaag tggtcaactg ctcgcgaaaa acctcgctgc
    15361 tgacgatcgc gacatccgaa gtttccgcga tcaagcgagc gatccgcatc tgtatcgggc
    15421 gcaaccagaa cgcgctctgc cgccaggatc ccgacgcata caactcgtga aaaacagtca
    15481 ccaatcgcgc ccggacccta atctgtagcc ggcgcagaac ggacggaagc cagacgggca
    15541 cgccccgccg attgaagccg tagttcacat agtgcaagac gatgccgccg taggtttcca
    15601 gccgccgccc gtcagcgacc gtccggaggg gggagaggag aggatagcca tcctcggtcc
    15661 tggcgctagc ggacggcgcg gcagaaatga aagtcgtttc aagatcgtga gattttttga
    15721 gacacccagc gaggatgcgg gcgtaatcgc cgactccgtc gcggccgccc ggcgcgtgcg
    15781 ggacgatctg caaaatcgtg ccggcctcac tcacgcgccg atcccgccag aggctggcct
    15841 aacgaatcaa gtgcgttgcg gtattgcttg atcgtgctgt gcgcgatgtc gcgccagcga
    15901 taaccggcgg cgatcgtcat gcactccgcg gaaagctttt cgtactccgg cgcactcagg
    15961 gcgagaatct cggaggcgcg ggccgcgagc gcggccgtat ctccgacggg cgtgagaaac
    16021 cgcacgcgtt gcgggtcgag aatctggcgc gggcccggca cgtcgtaggc gatcgtcggc
    16081 agaccggcgg ccaattgctc gaggaccgca agcccgaaac cctcgatgta gctgggaaac
    16141 aacgcgaccg cgcaatccgc caggagcgag ggcaggtccg actcctcaaa aatgggccgg
    16201 cagaaaatcc gattcgcctc agcggtctcc aggtccgagc gaacgactga ttcctcaaac
    16261 atcgttccga gaaagagaaa ttcgaccgcg ggatgacgct gccggatggc cgccataagc
    16321 ctgggccaat cgcgcgaacc cttgcgcagg ctccacattc cgatgaaaca gattctttgc
    16381 cggctcagac gctgttcggc tgggacggcg gcagtcgaga gggactccag aaaacgatcg
    16441 ttcagaccat acggctggac gatagacggg acccggacct gcggatcacg cgccagctcg
    16501 agacgctcgt cttcgttagg caaattgagc agatcagaat gccgcaatgc cttctccgag
    16561 tctttccacg agcgccacgc caaaaaacgg tgaaacaagc ggccaaacca ccgtcctcgc
    16621 ggttgatccg gccagagaat gcgcgattgc cgcagaaatt gatcgtaaag ccgatacagg
    16681 ccgaccgagc gagcgaccag caatccacgg aagcgaagcg agtttttccg aaatggcagc
    16741 actccaatga ggcaatcaat cacgtcgaaa cgttcggcgt tgcgccggac ataagccgcc
    16801 gcccggcggg gaaagcgcgc ctgccgcagc gcggaggcgg cgctggatcc cgccggtttg
    16861 gggtaggcat cggtgaggca aaacttttcc accgcgtgtc cggcccgagt ccattccttc
    16921 gccagttcga tccagacgcg cgcggcaccc aggcgcgaat cccacgggag attcacaacg
    16981 atcaggatac gaagcgggcc cgccgatggt ctcatcgggg tgaaactgcg ttccagaact
    17041 tgtggcgcac cggattcaca taatgccgca ggaccaggtg agagcccgcg taacgatccg
    17101 cgtaaacaaa ctgatcgtcc aaccgcaaga catacttctg ctcgtcgaag ggtaaggcgc
    17161 cattcgcgtg catgacgagc tggacgattg tctgcgtggt gaaaaaatcc gcagcgcctt
    17221 ccaactcgat caggcgctgg ataccgagcg accaatcgag gcgccggagc aacagcaaca
    17281 agccggcatt taccggcggc gaagattcat cgcggccacg caaaatgcgt gaatcgaccg
    17341 aataatccag gcagtccttg agatagaatg ccggcgcatt ttctcccctc gaagcccggg
    17401 cgatctcggc cgcgccggca aaaaaaagaa tgtccgcgtc aaggtacaat gccggcccgc
    17461 tgcacgggag cgacatgatc aggccgagtt gtttgcccag cggatgatcc gtgacgtaac
    17521 gacgaaactt ttccggtaaa tcggaaggcc ggtactcttc cgcacgtctg attgcaaccg
    17581 atggatcgat cttgctcagc aactccctgc tgcgctccga atgcgttcca tccgagacca
    17641 cggtaaaact ttggggacga cccgcgtggc gcaggaagga gcggatcgat cggacctgct
    17701 ccggcatcat tgcttccccg gagtaggaat agacatcgag ggggatcgag cggggggaac
    17761 acaccgtggt ctgcccgatc gcaggcagca acgcgcgata ggcggtccgc cgcaggccgc
    17821 ctgcccatcg ggcgcaatga tatccgaagt ttagcatctt atcccgccgg cagcggtcga
    17881 cgaaacccgc ggtaactcaa cccgaggttg agcgcaagac tcgggaatac agagcatagg
    17941 ttaaaggtga gcacaccgct cacactcgag aaatcagtga agggaatgag catcaactgc
    18001 gtcccgaggg tcagcggaat atagagccat gagccggcga tccaagccct tgaggcattc
    18061 aacgtccaga ccgtgctggc catcatgctg aggatggcac cgccgaccat caaaaggagt
    18121 tcgtgttgca ggtgtgagta cttgtttccg agcacgaaaa gaaattcgtt aggcagaaaa
    18181 gccgcaccgc tcagcacaag aagactgaaa cccgtaacgc cgccgaggat ccccgcgtaa
    18241 atccagcgga gctgccgcgg ctcctggcaa cgcgcgaagg caggggcaaa gatattggtc
    18301 aaaaggtttc ccatcaccac aaagatcacg gcgagccggc cgagcgctcc gacttcggcc
    18361 acggacgcgg cctgccggcc gaagaaggtg atcagaaaaa ccgtgacctg gccctgcagg
    18421 caaaagaaaa cg
//
LOCUS       DATMNS010000159         2067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4050936, whole genome shotgun sequence.
ACCESSION   DATMNS010000159 DATMNS010000000
VERSION     DATMNS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2067
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4050936"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2067)
                     /locus_tag="VNW28_05620"
     CDS             complement(<1..>2067)
                     /locus_tag="VNW28_05620"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXA09439.1"
                     /translation="MPPISTAERTLRFLGMPLARLIYRVTAVGTERLPSGGFLLLPNH
                     ITWVDAIVLLLACPRPIRFIIDQEYYENRFLHPILRTVGCIPITARRAKEAMRLAAEK
                     IREGEIVCLFPEGQLSRSGTLLRLQRGYEIIARRAEGQVVPVWLDQLWGSIFSFQGGR
                     FFTKWPRHFPYPATVEFGEPLSPNDADIATVREELLKIGERCYSRRDSLRGHLGAAAV
                     RGLARNPFRVAVIDGMDDSLLRRGKLLGAAAALSRHLRRHSPEHRIGIVLPASKGGVV
                     ANLAVVLAGKVPVGLNFTSGKDALQRAQQVAGLKTAISANAFANRLTDFPWPDNVIQL
                     DELLPRLKAKIFLWWMAAIFLPAPLLIRLLEIPREGGHAEAVLLFTSGSSGDPKGVVL
                     SHRNIIGNVSQFRVLLDATRDDLILATLPVFHSFGCTVTLWFPLLDGIRTVTFPNPLD
                     TAKCAELIAQYRVTIVLAAPTFLRGYLRKAEAAQLASVRLTITGAEKLPRSLAEAFEE
                     RFGKTVYEGYGLTETSPVVSVNLPDPEPDHPGDPVQASHRPGSVGRMAPGIAGEIRDP
                     ETGAKLSLHDSGMLWLRGPNIFEGYLDDPRRTADVMRDGWLVTGDLGRFDEDGFLFIE
                     GRLARFSKIGGEMVPHETVEEKILAALGLSSEDARTIAVASVTDAQKGEALVLLSTIE
                     IDPGQLR"
BASE COUNT          426 a          646 c          645 g          350 t
ORIGIN      
        1 gcgcaactgc cccggatcga tttcgatcgt gctcaagagg acgagcgctt cgcccttctg
       61 ggcgtcggtg accgacgcga ccgcgatcgt gcgggcgtcc tcactgctga ggccgagcgc
      121 ggcgagaatt ttttcctcga ccgtttcatg cggcaccatt tcgccgccga ttttagagaa
      181 gcgtgcaagc cgtccttcga tgaaaaggaa gccgtcctca tcaaaacgcc caaggtcgcc
      241 cgttaccagc cacccatcgc gcataacgtc cgccgtccgt cgcggatcgt cgaggtaacc
      301 ttcaaaaata ttcggcccgc gcagccagag catgccggaa tcatgcagcg agagtttcgc
      361 gccggtttcc gggtcgcgaa tttcccccgc gataccgggc gccattcgcc cgactgaccc
      421 cggccgatgc gaagcttgca ccggatcgcc cgggtgatcg ggctccgggt cgggcagatt
      481 cacacttacg accggcgaag tctcggtcag gccgtagccc tcgtagacgg tcttgccgaa
      541 acgctcctcg aaagcttcgg ccaggctgcg cggcaatttc tccgcccctg tgatcgttag
      601 gcgaacgctg gccagttgcg ccgcctccgc cttgcgcaga tagccgcgca gaaaagtggg
      661 cgccgcgagc acgatcgtga cgcgatactg cgcgatcaac tcggcacatt tcgccgtgtc
      721 cagcgggttg ggaaaagtca ccgtgcggat gccgtcgagg agaggaaacc agagcgtgac
      781 ggtgcaacca aagctgtgga aaacggggag cgtggcgagg atcaaatcgt cgcgcgtggc
      841 atcgagcagg acccgaaatt gcgagacgtt gccgatgatg ttgcggtgac tcaggacgac
      901 gcctttcgga tcgccggagc tgccgctggt aaagagaagg accgcttcgg catggccccc
      961 ttcccgcggg atttcgagca gccgaatcaa gagcggggcg ggcagaaaga tcgccgccat
     1021 ccaccagaga aagatcttcg ctttcaggcg gggcagcaat tcgtccagtt ggatgacgtt
     1081 gtccggccag ggaaaatccg tcaaccgatt ggcaaacgcg ttggcggaaa tcgccgtttt
     1141 taatcccgca acctgctgcg cgcgctgcaa cgcatctttc ccgctcgtaa aattcaggcc
     1201 caccggaacc ttgccggcca gcaccaccgc gaggttggcg accactccgc ccttgctcgc
     1261 cggcaaaacg atgccgatcc gatgctccgg agaatgtctg cgcaaatgcc ggctcagggc
     1321 cgccgcggcg cccagcaatt tcccgcgccg caggagtgaa tcatccatcc cgtcaatcac
     1381 cgcgacgcgg aacggattcc gggccagacc ccgcacggcg gcggctccga gatgaccgcg
     1441 caacgaatcg cgccggctgt agcagcgttc cccgattttg agcaattcct cgcgcaccgt
     1501 cgcgatgtcg gcgtcgttag gcgaaagcgg ttctccgaat tccaccgtcg ccggataggg
     1561 gaaatggcgg ggccacttgg tgaagaaacg cccgccctgg aaggaaaaaa tcgagcccca
     1621 caattgatcg agccagacgg gtacgacctg gccctcggcc cggcgcgcga tgatttcgta
     1681 gccccgctgc agtcggagca aagtgccgga gcgcgacaac tgaccctccg gaaagagaca
     1741 aacaatttcg ccttcgcgga ttttttcggc ggcaagacgc atggcttcct tggcccgccg
     1801 cgcggtgatc gggatgcaac caaccgtgcg caggatcgga tggaggaagc gattttcgta
     1861 atattcctgg tcgatgatga aacggatcgg acgggggcag gcgagcagga ggacgatcgc
     1921 atccacccag gtgatgtggt tgggcaaaag caaaaatcca ccggacggaa gccgctccgt
     1981 gccgaccgct gtgacgcgat agatcagccg cgcgagcggc atcccaagaa aacgcagggt
     2041 ccgctcggcg gtggaaatgg gtggcat
//
LOCUS       DATMNS010000160         2573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2732166, whole genome shotgun sequence.
ACCESSION   DATMNS010000160 DATMNS010000000
VERSION     DATMNS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2732166"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1755
                     /locus_tag="VNW28_05625"
     CDS             <1..1755
                     /locus_tag="VNW28_05625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA09440.1"
                     /translation="KVLPPRAALVTRRTLVRLLDDLASQEPPGPLGEMQQQNRELLTT
                     LEELAARQEELVRLNSELEDTNRGVVALYAELDDKADHLRRADEMKSRFLSHMSHEFR
                     TPLNSILALTRLLLNRADGDLTGEQERQVFFIKKSAESLFELVNDLLDLAKVQAGKTT
                     VLRAELDVKNLFGALRGMLRPLLVNPSVNLIFEEPEDLRMVSDEGKISQILRNFISNA
                     LKFTERGEVRVAARRSANSLIFSVSDTGIGIDPGDLERIFQEFTQLDSPIQQKVKGTG
                     LGLPLSRKLAELLGGTVNVVSSPGLGSTFSLTLPVGNVAPSSDPQPSQTNDIVPVLVV
                     EDHDETRMIFEDYLKGSQYHLIPARDVSQARAAIEAIRPRAILLDAMLANQEGSTLLA
                     ELKARKDTMDIPVLAIGNVEDLSMVSRADAYAPKPVSKQWLLESLDRYTRPALNTRRI
                     LLVDDDPTFRYVVKQLFSGMPHEFIEADTGVAGLEAAISRDPELIFLDLMMPQMTGYE
                     VLDRLRADPKTRETPVIIVSSRFLNDRELRQLHGLGAVTFSKDRFGDGDIISAVREIL
                     EGFGLADLLPDAGRQVKT"
     gene            1752..>2573
                     /locus_tag="VNW28_05630"
     CDS             1752..>2573
                     /locus_tag="VNW28_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA09441.1"
                     /translation="MMQQRERPLILNVDDNDVGRYTVTRELRRMGYDVLDAGNGGDAL
                     ELAGSAKPDLVVLDIHLPDIDGFEVCRRLKTDPRTASILVLHLSASYLDDVSRLKGFD
                     SGADGYLTEPLDPAVLSATVKALLRMKEAERAERIAAQRWQATFDAIADGVAIVDSDG
                     TILSTNRALSAITGRTPADLIGISWYVLFPAEPAECVLRTMFESGKREVLEHAYGDQV
                     LRISCDPIRGDLGAITGCVAIASDITDWHKLQEALRHTQKLESIGLLAGGIAHDFN"
BASE COUNT          528 a          812 c          718 g          515 t
ORIGIN      
        1 aaggtattgc ctcctcgcgc cgccctcgtc acgcggcgaa ctctggtccg cctgctggac
       61 gatctggcca gccaggagcc gccggggccc ctcggcgaaa tgcagcagca gaatcgcgaa
      121 ctactcacta ccctggagga acttgccgcc aggcaggaag aactggtgcg cctcaacagt
      181 gagctcgaag acactaaccg tggggtggtg gccctgtacg cggaactcga cgacaaagcc
      241 gaccatctgc gccgtgccga cgagatgaag tcgcgcttcc tctcccatat gagtcacgag
      301 ttccgtaccc ctctcaactc catcctggcg ttgacccgac tgctgctcaa tcgcgccgat
      361 ggggacctca ccggcgagca ggagcgccag gtctttttca tcaagaaatc cgccgagagc
      421 ctttttgagt tggtcaacga tctcctcgat ctggccaagg tgcaggccgg gaagaccacc
      481 gtcctgcggg ctgagctcga tgtgaagaat ctcttcggcg ctttgcgcgg catgctgcgg
      541 cccctcctcg tgaacccctc ggtcaacctt atcttcgaag aaccggaaga ccttcgcatg
      601 gtctccgatg aggggaagat ctcccaaatc ctaagaaatt ttatctccaa cgcgctcaaa
      661 ttcaccgagc gcggcgaagt ccgcgttgcc gcccggcgtt ccgcgaattc cctgatcttc
      721 tccgtctccg atacaggaat cgggattgac ccgggcgatc tggagcgcat cttccaggaa
      781 tttacacagc tcgacagccc cattcaacag aaggtcaaag gtactgggct aggccttccg
      841 ctctcccgta aactcgcgga gttgctcggc gggacggtga atgtagtgag cagccccggt
      901 ctcggatcta ccttctcgtt aactctcccc gtgggaaatg ttgccccttc gtcggatccc
      961 caaccatctc aaaccaacga tatcgtacct gtcctcgtgg tagaagacca cgacgaaacc
     1021 cggatgatct tcgaagacta cttgaagggt agtcaatatc acctgatccc cgcgcgcgac
     1081 gtgagccagg cccgtgccgc gatcgaagcg attcgtccgc gagcgatttt actcgacgcc
     1141 atgctcgcta accaggaggg ttccacgcta ctcgccgagc ttaaggcccg gaaggacaca
     1201 atggacattc ccgtgctggc gattggtaat gtagaagacc tgagcatggt ctctagagcc
     1261 gacgcttatg cgccgaaacc cgtgagtaaa caatggctcc tggaatcgct ggatcggtat
     1321 acccggccgg ccctgaatac tcgccgtatc ctcctcgtgg atgacgaccc tacgtttcgg
     1381 tacgttgtga aacaactctt cagcggcatg ccgcatgagt tcatcgaagc cgacacgggc
     1441 gttgccgggc tggaggccgc aattagccgg gatcccgagt taatctttct ggacctgatg
     1501 atgccgcaaa tgaccggcta cgaggtgctc gaccggttaa gggccgatcc aaaaacccga
     1561 gagacgcctg tcatcatcgt cagttctcgc ttcttgaacg atcgtgagtt gcgccaactc
     1621 catggcttgg gtgcagtaac cttttccaag gaccgcttcg gcgatggcga cataatctcc
     1681 gccgtccgcg agatactgga ggggttcggt ttggccgacc tcctgcccga tgccggccgg
     1741 caggtcaaga catgatgcaa caacgcgagc gcccgttgat tctcaatgtc gacgacaacg
     1801 atgtcggccg ttacaccgtc acgcgcgaat tgcgccgcat gggctacgat gtactcgacg
     1861 ccggcaacgg cggcgacgcg ctagaattgg cgggctccgc caagcccgac ctcgtggtgc
     1921 tcgatattca ccttccggat attgacggct tcgaagtttg ccgtcgtctc aaaacggacc
     1981 cgcgcacggc cagtattttg gttttacatc tctccgcctc gtatctcgac gacgtttcgc
     2041 gtctgaaagg tttcgatagc ggcgccgatg ggtaccttac cgaacccctg gatccagccg
     2101 ttttgagcgc gaccgtgaag gcgctgctcc gcatgaagga ggccgagcgc gccgagcgca
     2161 tcgccgccca gcgctggcaa gccacattcg acgccatcgc cgacggagtc gccatcgtgg
     2221 actccgacgg aaccatcctg agcaccaatc gcgcgctgtc cgccataacc ggccgcaccc
     2281 ccgcggattt gatcggcatt tcatggtacg tgcttttccc cgccgaaccc gccgaatgcg
     2341 ttttgcggac gatgttcgaa agcggcaaac gcgaagtgtt ggagcatgcg tatggcgatc
     2401 aagtactgcg catctcctgc gaccccatcc ggggagactt gggcgcgatt accggatgcg
     2461 tcgccatcgc cagcgacatc accgattggc acaaactgca ggaagcgctt cgacacactc
     2521 agaagttgga aagcatcggc ctgctggccg gaggcatcgc ccacgatttc aat
//
LOCUS       DATMNS010000161         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_943037, whole genome shotgun sequence.
ACCESSION   DATMNS010000161 DATMNS010000000
VERSION     DATMNS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943037"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1448)
                     /locus_tag="VNW28_05635"
     CDS             complement(<1..1448)
                     /locus_tag="VNW28_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXA09442.1"
                     /translation="MHRRLVTFFILAAFPAAALRAAAVPVASPPSAQPAPTIPEEAAA
                     PGKDVVHQLNNAFAKVFEVVAPSVVIIEVTKKADGNDTSLDDLFFQGPDENDPHHDRR
                     LPDPVQSEGSGFIVRPDGFIYTNFHVVESADKVEVKLKDGREFPGKIIGTDEKTDIAV
                     VKVDATNLPVVQLANSDAVRVGQFAFAIGAPFKLDYTFTYGVISGKGRSKLLETGGYN
                     IADYLQTDTSINPGNSGGPLCDIDGKVVGMNTLINGLNRGLGFAIPSNLVNEIGTQLI
                     AGTKIVRPWLGIRIETLGDDSSIRDLFKGVDKGVVVRTIEADAPAYKSDLRPFDLITQ
                     IDGAPVTTDTQLQHEILKKKVGQNVELGVWRKGQMMKIAVTTGELPNDIGRAPAQPAP
                     PTEPLQEDVNRFGLQVQDLTKEVATRLKLSVQQGVIVTDVADNSIAAAQDIRREDVIT
                     EVDGKPVTSVASFRDALTKADPRRGILLYLDR"
     gene            1685..>2408
                     /locus_tag="VNW28_05640"
     CDS             1685..>2408
                     /locus_tag="VNW28_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HXA09443.1"
                     /translation="MLTLAEGKKDGKEFISMAPGYFPDIAPELWNDWKWQLKNRVTTL
                     AQLEQHLALSAEERAGVLLSGDKLALAITPHFFNLIERDNPDCPVRRQVVPRIEETWA
                     SPYDMADPCGEDSHMPVPGLVHRYPDRVLFLVTDRCASYCRYCTRSRVVSGVGEQELH
                     TEFEAAFKYLEEHTEVRDVLLSGGDALLFSDAKLEKMLSRLRAIPHIEFLRIGTRVPI
                     FLPQRITPEFCAMLQKYHPLWMS"
BASE COUNT          449 a          759 c          704 g          496 t
ORIGIN      
        1 ttgcgatcga gataaagcag aatcccacgg cgcggatcgg ccttcgtcag ggcgtcgcga
       61 aagctggcca cacttgtaac cggcttgccg tccacctccg taatcacgtc ttcgcgccgg
      121 atgtcctgcg cggcggcgat gctgttatcg gccacgtcgg tcacgatcac gccctgctgc
      181 acgctcaact ttagccgcgt cgccacttcc ttcgtcagat cctgcacctg caggccaaag
      241 cggttgacgt cctcctgcag cggctcggtt ggtggcgccg gttgcgccgg ggcgcggccg
      301 atgtcgttag gcaattcgcc cgtcgtgacc gcgattttca tcatttgccc cttgcgccag
      361 accccgagct cgacgttttg cccgactttc tttttcaaaa tctcgtgctg caactgcgtg
      421 tcggtcgtga ccggtgcgcc atcgatctgg gtgatcaagt cgaacggccg caggtcgctc
      481 ttgtaggccg gcgcgtccgc ctcaatcgtg cgcacgacca cgcccttgtc gacgcccttg
      541 aacaaatcgc ggatcgaaga gtcgtcgccc agggtttcga tccgaatgcc gagccacggc
      601 ctcacgatct tggttccggc gatcagttgc gtgccgatct cgttcaccag gttgctcgga
      661 atcgcgaacc caaggccccg gttgaggccg ttaatcagcg tgttcatgcc gaccactttc
      721 ccgtcgatgt cgcaaagtgg gccgccactg ttacccggat tgatcgaggt gtcggtctgc
      781 aaataatccg cgatgttata gccgccggtc tcgagcaact tgctccggcc ttttccgctg
      841 atcaccccgt atgtgaaagt atagtcgagc ttgaagggcg cgccgatcgc gaaggcaaat
      901 tgcccgaccc gcaccgcgtc gctgtttgcc aactggacga ccgggagatt ggtcgcgtcc
      961 actttcacca ccgcgatatc ggtcttctca tcggtgccga ttatcttgcc ggggaattcg
     1021 cggccatctt tcaacttcac ctccactttg tccgcgctct cgacgacgtg gaaattggta
     1081 tagatgaaac catccggtct cacgatgaaa ccggagcctt cgctttgcac cgggtcgggc
     1141 aggcggcggt catgatgcgg gtcgttttca tccggtccct ggaaaaaaag atcgtccagc
     1201 gaggtgtcgt tgccgtcggc cttctttgtc acctcgatga tgacgacgct cggggcgacc
     1261 acctcgaaca cctttgcgaa ggcgttattg agctggtgaa caacatcctt gccgggcgcg
     1321 gccgcctcct ccgggatggt cggcgccggt tgggcgctgg gaggagatgc aactggcacc
     1381 gctgccgccc gcagagcggc ggcgggaaaa gccgcgagaa tgaaaaaggt aacaaggcga
     1441 cgatgcatag gcggcgattt ggacaggagc gcgggcggcg ctgttcaaat agtagcggag
     1501 agcgggacgc gggcaaaccg ctccgcgcaa atccttctcc tgcttttgct cctaatcctg
     1561 ctccctggcc gataggagcc cgggcaggaa caagattcgg ggagagcgga gtgcgtcacg
     1621 cacccgaaag tgccctcatt gacacgaaat ttaaggccct tagcttttct ccctcaaatc
     1681 taaaatgctt actcttgccg aaggcaaaaa ggacggaaaa gagttcatct cgatggcccc
     1741 cggttatttc ccggacatcg cgccggaact ctggaacgat tggaaatggc agctcaagaa
     1801 tcgcgtcacc accctggcgc agctcgagca gcatctcgcg ctctccgcag aagagcgcgc
     1861 gggcgtgctg ctctcgggcg acaagctcgc gctcgccatc acgccgcatt tcttcaacct
     1921 gatcgagcgc gacaatcccg actgcccggt ccggcgccag gtggtcccgc ggatcgagga
     1981 aacctgggct tcgccttacg acatggcgga cccatgcggc gaagactcgc atatgccggt
     2041 gcccggactc gttcatcgtt accccgatcg agtgcttttc ctcgtgacgg atcgttgcgc
     2101 gagctactgc cgctattgca cgcgcagccg cgtcgtcagc ggggtggggg aacaggaact
     2161 gcacaccgag ttcgaagccg ccttcaagta tctcgaagag cacaccgaag tgcgcgacgt
     2221 cctgctttcc ggcggcgacg cgcttctctt cagtgacgcg aagctggaaa aaatgctgag
     2281 ccggctgcgg gccatcccgc acatcgaatt tctccgcatc ggcacccgcg tgccgatctt
     2341 cctcccgcaa cgcatcacgc cggagttctg cgcgatgctg caaaagtatc atccgctttg
     2401 gatgagca
//
LOCUS       DATMNS010000162         9087 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_577336, whole genome shotgun sequence.
ACCESSION   DATMNS010000162 DATMNS010000000
VERSION     DATMNS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9087)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577336"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="VNW28_05645"
     CDS             <1..1558
                     /locus_tag="VNW28_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXA09444.1"
                     /translation="FGIKIPTQYGGLGLSQMNYGRAAMLLGSWDANLTALVSAHQSIG
                     VPQPLLIFGTEEQKTKYLPRVARKEISAFALTETDAGSDPANMSLRADATEDGSAFIL
                     NGEKLWCTNLIKAGVLVVMAKTPPKMVKGRERKQITAFIVDVDSPGLEITYRCHFMGL
                     RALYNGIVKFTNVRVPRENMIAKEGQGLKVALTTLNTGRLTIPAACVGLSKRLLEICR
                     KWASERVQWGVPIGQHYAIAGKIAEMAGNVFAMEAMTFLTSALVDRKAGDLRIETAMC
                     KMWATEATWRIADEAMQVRGGRGYETAQSLEGRGEDGIPVERFLRDCRINMIFEGSSE
                     IMRLFIAREALDPHLKIGGAIFNTQLPTSERARAVFKSGKFYASWYPKQWMGASAGDL
                     DKLHADLRPHVRYAAGASKRLARGLFHAMTRFGPKLDREQLLLARFVGIATELFAMSA
                     TCAFAQHKIESGEPVAEILSVATYFCRSARMRIDTQFAGVSLNADRAGYDLTQELLAG
                     KHADLRQGIV"
     gene            1656..3050
                     /gene="lat"
                     /locus_tag="VNW28_05650"
     CDS             1656..3050
                     /gene="lat"
                     /locus_tag="VNW28_05650"
                     /EC_number="2.6.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR03251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0045484 - L-lysine 6-transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine 6-transaminase"
                     /protein_id="HXA09445.1"
                     /translation="MSMPSMLTEAARTPSGNRISASEKSKTESKSRVLEQIAEHVLLD
                     GFRIVIDLEKSQGSYLYDSAHDRRLIDLYGFFGSNPVGFNHPHFDQPEVKADLLRAAK
                     IKIANSDVYSDGYARFLDTYWRVVGMAPLERMLLIEGGALAVENTLKTAMDWKVRKNM
                     AAGRGERGTEILHFRHAFHGRSGYTMSLTNTDPVKTDLFAKFDWPRVSTPCIDFSLAE
                     PERTRDVIEREKKSEQEIMQRVKERGVDIAAIIIEPIQGEGGDNHFRGEWLQTLRRIC
                     DEHEMLLIFDEVQCGMCTTGRNWCCQHFGVMPDLMAFGKKAQVCGVMAGPRLDEVTDN
                     CFRVPSRINSTWGGNFTDMVRSTHFLQIIEAENLVENARIVGEHFLNRLLELQEEEAM
                     FTAVRGRGLFLAFDLPDPATRNEFWKGLFDGGVLALKSGERAIRFRPALDIRKEVVDE
                     AIDLLRQQCRRMRQ"
     gene            3053..4600
                     /locus_tag="VNW28_05655"
     CDS             3053..4600
                     /locus_tag="VNW28_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXA09446.1"
                     /translation="MTELLAQLGISGTAEGAFSGEWHGGGAVHEKISPVDGKSLGKFR
                     TASPADYDKVMSRAQEAFTRWRTTPGPVRGETVRQLGNALREQKDDLGKLVSLESGKI
                     LAEGKGEVQEMIDICDFAVGQSRMLYGLTIQSERPNHRLMEQWHPLGVIGVISAFNFP
                     VAVWSWNAALAAVCGDATVWKPSEKTPLSAIAVIKIAERVCRATGADPAIFTLLIGDR
                     HSVGEPLAADRRIPLVSATGSTAMGIAVAKSVHGRLGRSILELGGNNALVVCPSADLE
                     MAAQSIFFGAVGTAGQRCTSTRRVIAHESIADKLREKLLAAYKSVPIGNPLDEKTLMG
                     PLIDRQAVDTVLDAIKKLKAEGGEVLYGGERLDGDQYAGGCYMTPCLAKAKPDYKIVH
                     DETFGPLLYLLTYRDFDEAIAINNSVPQGLTSAIFTNDVREAEKFLSPAGSDCGITNV
                     NTGTSGAEIGGAFGGEKETGGGRESGSDSWKMYMRRTTNTINYSSELPLAQGIRFGGP
                     EEGSATA"
     gene            4634..4843
                     /locus_tag="VNW28_05660"
     CDS             4634..4843
                     /locus_tag="VNW28_05660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09447.1"
                     /translation="MAILIDETKRVLVQGITGREGPARTRLMREYGTKVVAAVTPEQT
                     REKPMGRIDPVKHGYDGPAAQSVAK"
     gene            4840..5133
                     /locus_tag="VNW28_05665"
     CDS             4840..5133
                     /locus_tag="VNW28_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09448.1"
                     /translation="MKAQKIEKKILTKHPLGKSGKNISQDKYELAKKTILTALRTGQL
                     THTELFAELNKSLASKFTGNINWYGETVKLDLEARKIIERTSSKPPKYRLNKA"
     gene            5388..6785
                     /locus_tag="VNW28_05670"
     CDS             5388..6785
                     /locus_tag="VNW28_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09449.1"
                     /translation="MSNAELLRKMEALEAKVAGLESRLAEYENGGEKNKSAGAKLKAN
                     PYADDASGPQGQQSMTARSETAASPNDRSLFGLGYRSQAILSIGAYGELKFGGQQTPE
                     GWKDGFDAGRIVLLPTYQIADNIIFNAEIEFEHGGIADDADDKLTGAVELEQAYIDFR
                     FNEHFNWRAPGVDVVPFGFINLFHEPTQFYSVDRPELDNGLIPTTWFEGSTSIYGKIV
                     DNLNYQFQINTGLEDLGENCGVPEGPYEAGISGTEALALARTPIGDFNQTKDALGYTL
                     RLSYTPPFIPGLAGSTSVFFTPNVTPRGAHSDDGRSLGNGSVTMFDTEVRYRIPKTGF
                     EFRGEFVDVLLGSPVNLRANNDGDPENNVGNSMWGFSLEAAYHIDLGDRFRNGWELVP
                     FYRYTYENLQTGGFAGSDENLPTGQGRRQFHTIGLALFPTPQVVLKIDYQFALDNAPD
                     SPRADHLLGAVGFFF"
     gene            6811..7350
                     /locus_tag="VNW28_05675"
     CDS             6811..7350
                     /locus_tag="VNW28_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HXA09450.1"
                     /translation="MDQFPRWILPAAISVTVPLPCYAVQYLTVPAAQKLCFPDCDRFV
                     AAHVKFTPAQIHAIEAASGQKELLRGQEIWRATRSGKLQGFFIVDYVIGKHLVIDYAV
                     ALSPSGAVRQIEILNYRESYGGEIQNPEWRKQFVGKTSRSHFALNDGITNISGATLSS
                     RHVTEGVRRVLATYEICLK"
     gene            7331..8209
                     /locus_tag="VNW28_05680"
     CDS             7331..8209
                     /locus_tag="VNW28_05680"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HXA09451.1"
                     /translation="MKSVSSEVRRARPLLGTFVEITAQGRDETTLHRAINDAFAAVRQ
                     VHDLMSYHDPESDVSRLNRHGFVQNVTVHPWTWQVLQAAGEFSRQSGGVFDITVAPEL
                     TRRNYLPPSGYDSDASSTWRDVSLGENGRTFFRRRATIDLGGIAKGFAVDRAVEALVA
                     GGAQSGIVNAGGDLRVFGPEPRLVHIRHAQDSRAIGGAIMLRESSLATSALYGREGDH
                     RQTGALIDGRTGCPALGPVSVSVYAPDCMTADALTKIVLALRDDARPILDRYRAGAFI
                     FVGDSSFCELRSRQAT"
     gene            8181..8693
                     /locus_tag="VNW28_05685"
     CDS             8181..8693
                     /locus_tag="VNW28_05685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09452.1"
                     /translation="MNSAADRRHNGIRLGRRHEWSVYVVLAIVFSSGVVWAWLHHFAR
                     RAGEFGESGHPAELWMQKVHGASAMFALIVLGSLLVFHIPRAWRAKRNRPTGSSLFAL
                     FAFLAGSGYALYYSGGESLRAWMSWSHLWVGIALPVFVALHVWRGKRATAPKSLEKSV
                     PGMAARNNFR"
BASE COUNT         1913 a         2666 c         2738 g         1770 t
ORIGIN      
        1 gtttggcatc aaaatcccga cccagtacgg cgggctcgga ttatcccaga tgaactatgg
       61 gcgggccgcg atgttgctcg gcagctggga cgcgaatctt accgcgctcg tctcggcgca
      121 tcagtcgatc ggtgtcccgc agccgctcct cattttcggc acggaagagc agaaaacgaa
      181 gtatttaccc cgggttgcgc ggaaggagat ttcggctttc gcccttaccg agacggatgc
      241 cggttccgat cccgcgaaca tgagtctgcg cgcggatgcg accgaggacg gctccgcctt
      301 tatcctgaac ggcgaaaaac tctggtgcac caacctgatc aaggcgggcg tgctcgtcgt
      361 catggcgaag acgccgccga agatggtcaa gggccgggaa cgcaaacaga tcacggcttt
      421 tatcgtcgat gtcgattcgc ccgggctcga gatcacttat cgctgccact tcatggggct
      481 gcgcgcgctc tataacggga tcgttaaatt caccaacgtt cgggtgccac gcgaaaacat
      541 gatcgcgaag gaagggcagg gattgaaggt tgcgctcacg accttgaaca ccggtcgcct
      601 gacgatcccg gcggcctgtg ttggtttatc caaacgcctg ctcgagatct gccgcaagtg
      661 ggccagcgaa cgcgtgcaat ggggcgtgcc gatcggccag cactacgcga ttgcgggcaa
      721 gatcgcggaa atggcgggga acgtcttcgc gatggaagcg atgaccttcc tgacgagcgc
      781 gctggtcgat cgcaaagcgg gcgacctgcg gatcgagacc gcaatgtgca aaatgtgggc
      841 gacggaagcg acctggagga tcgccgacga agcaatgcag gtgcgcggcg gtcgcggtta
      901 cgagactgcg caatcgctcg agggccgcgg cgaggatggc attccggtgg agcgtttcct
      961 gcgcgactgc cgcatcaaca tgattttcga gggatcgagc gaaatcatgc gcctcttcat
     1021 cgcgcgggaa gcgctcgatc cgcatctcaa gatcggcggc gcgatcttca acacccaatt
     1081 gccgacttcg gagcgggcca gggcggtttt caaatcaggg aaattctacg cgagctggta
     1141 tccgaaacag tggatgggtg cgagcgcggg cgacctcgat aagttgcacg ccgacttgcg
     1201 cccgcatgtg cgttacgccg ccggcgcgag caaacggctg gcccgcggtt tgttccacgc
     1261 gatgacgcgc ttcggcccca agctcgatcg cgaacaattg cttctcgcgc gcttcgtcgg
     1321 catcgcgacg gagcttttcg cgatgagtgc gacctgcgcc ttcgcgcagc ataagatcga
     1381 gagcggcgag ccggtggctg aaattttgtc ggtcgcgact tatttctgtc gttccgcgag
     1441 aatgcggatc gatacccagt ttgccggagt ctcgctcaac gcggatcgcg ccggctacga
     1501 cctgacccag gagttgctcg ccgggaaaca cgccgacctg cggcagggga ttgtgtaaca
     1561 cagccgtccc ggctgtcgcg tagcgcgggc gtctcgcctg caggcgggaa gcccgcccga
     1621 ctcgacaggg tggaagcctg tgttaccctc agcggatgag tatgccatcc atgctcaccg
     1681 aagcggctcg gactccttcc ggcaaccgca tcagtgcgag cgaaaaatca aaaaccgaat
     1741 cgaagtcccg ggtcctcgag caaatcgccg agcatgtctt gctcgacggt tttcgtatcg
     1801 ttatcgacct cgaaaagagt caaggctctt acctttacga ttcggctcac gatcggcgac
     1861 tgatcgatct ctacggattt ttcggatcaa atccggttgg ttttaatcac ccgcatttcg
     1921 atcagccgga ggtgaaagcg gacctgcttc gcgcggccaa gatcaaaatc gcgaactccg
     1981 acgtttactc cgacggctac gcgcgtttcc tcgataccta ctggcgggtg gtcgggatgg
     2041 cgccgctcga gcgcatgctc ctgatcgaag gcggcgcgct cgcagtcgag aacacgctca
     2101 agaccgcgat ggattggaag gtgcgcaaga acatggcggc cggtcgcggc gaacggggga
     2161 cggagattct ccactttcgc cacgcctttc acggccgcag cggctacacc atgagcctca
     2221 cgaacaccga tccggtgaag acggatttgt ttgcgaaatt cgactggccg cgcgtttcca
     2281 cgccgtgcat tgacttttcg ttagccgaac cggaacggac ccgcgatgtg atcgagcgcg
     2341 aaaagaaatc ggaacaggaa atcatgcagc gcgtcaaaga acgcggcgtc gatatcgccg
     2401 cgatcatcat cgagccgatc cagggcgaag gcggggataa tcatttccgc ggcgagtggc
     2461 tgcaaacgct ccgccgtatc tgcgacgagc acgagatgct cctcattttc gacgaagtgc
     2521 agtgcgggat gtgcaccacc ggtcgcaact ggtgctgcca gcatttcggc gtcatgcccg
     2581 acctgatggc gtttggcaag aaggcccagg tctgcggcgt gatggccggc ccgcgcctcg
     2641 atgaagtgac ggataactgc tttcgcgtgc cgagccggat caattcgacc tggggaggaa
     2701 acttcaccga catggtgcgc tcgacccatt tcctccagat catcgaggcg gaaaatctgg
     2761 tcgagaacgc gcgcatcgtc ggcgaacact tcctcaatcg cctcctcgag ctgcaggaag
     2821 aagaagcgat gtttaccgcc gtgcgcggtc gcggtttgtt tctagccttc gatctgccgg
     2881 acccggcgac gcggaatgaa ttctggaaag ggctgttcga cggtggcgtg ctcgcgttga
     2941 aatccggcga acgcgcgatc cgcttccggc cggcgctcga catcaggaaa gaagtcgtcg
     3001 acgaagcgat cgatctgctc cggcagcaat gccggcggat gcgccagtag ccatgacgga
     3061 acttctcgcg cagctgggca tctccggcac ggctgagggc gctttctctg gcgaatggca
     3121 cggcggcggc gcggtgcatg aaaagatttc gccggttgat ggcaagtcgt taggtaaatt
     3181 ccgcacggcc tcgccggccg attacgacaa ggtgatgagc cgcgcgcagg aagctttcac
     3241 caggtggcgc accacgcccg gaccggtccg cggcgaaacc gtccgacagc tcggcaacgc
     3301 tctgcgcgaa cagaaggacg atctgggcaa gctcgtctcg ctcgagtcgg gcaagattct
     3361 cgccgaaggc aaaggcgaag tgcaggagat gatcgacatc tgcgatttcg ccgtgggcca
     3421 atcgcgcatg ctttatggcc tcacgattca atcggagcgc ccgaaccatc ggctgatgga
     3481 gcagtggcat cctctcggcg tcatcggtgt catctccgcc ttcaattttc cagtggccgt
     3541 ctggtcgtgg aacgcggccc tggccgcggt ctgcggcgat gcgacggtct ggaagccgtc
     3601 ggagaaaacg ccgctcagcg ccatcgccgt gatcaagatc gcggagcgcg tttgtcgcgc
     3661 gacgggcgcg gacccggcga ttttcactct cctgatcggc gaccgccaca gcgtcggcga
     3721 accgctcgcg gctgatcggc gcattccgct cgtctccgcc accggctcga cggcgatggg
     3781 aatcgcggtc gcgaaatcgg tccacggccg gctcggacgg agcatcctcg aactcggcgg
     3841 caacaacgcg ctcgtcgttt gcccgagcgc cgatctcgag atggcggcgc aatcgatttt
     3901 cttcggcgcc gtcggcaccg cgggccagcg ttgcacttcc actcggcgcg tcatcgcgca
     3961 tgagtcgatt gccgacaaat tgcgcgagaa actcctcgcc gcctacaaat cggtcccgat
     4021 cggaaatccg ctcgacgaaa aaacgctgat gggtccgttg atcgatcgtc aggccgtcga
     4081 caccgtcctc gatgcgatca agaaactgaa ggccgagggc ggcgaagtcc tttacggcgg
     4141 cgagcggctc gacggcgacc agtatgccgg cggctgttac atgacaccct gcctcgcgaa
     4201 ggcgaaaccg gattacaaga tcgtccacga cgaaaccttc gggccgctcc tttacctcct
     4261 gacctatcgt gattttgacg aagcgatcgc gatcaataac agcgtcccgc agggtctgac
     4321 ctcggcgatt ttcacgaatg acgtgcgcga ggcggaaaaa ttcctcagtc ccgccgggag
     4381 cgattgcggg atcaccaacg tcaacaccgg cacgagcggc gcggaaatcg gcggcgcctt
     4441 tggcggcgaa aaagagaccg gtggcggccg cgaaagcggg agcgattcct ggaagatgta
     4501 catgcggcgg acgacgaaca cgatcaacta ctcgagcgag ctgccgctcg cgcaaggcat
     4561 ccgctttggc gggccggaag aaggcagcgc caccgcctaa cgaatggcct tttaaagcag
     4621 agcggaggat caaatggcca tcttgatcga cgagacgaaa cgcgtgctgg tgcaaggcat
     4681 caccggccgg gaaggcccgg cgcgcactcg gttgatgcgc gaatacggaa ccaaagtggt
     4741 cgccgctgta accccggagc aaacgcgaga gaaaccaatg gggcggatcg atccggtgaa
     4801 acatggttac gacgggccag ccgcacagag tgtggcaaaa tgaaggcgca gaaaattgag
     4861 aaaaagattc tgaccaaaca cccgttaggc aaaagcggga agaacatcag ccaggataaa
     4921 tatgagctgg cgaaaaagac gatcctcacc gcgttgcgga ccgggcaact gactcacacc
     4981 gaattgttcg cagagctaaa taagagcctg gcgagcaagt ttacggggaa catcaattgg
     5041 tatggcgaaa ccgtgaagct cgatctggaa gcgcggaaaa tcattgaacg cacttcctcc
     5101 aagccgccaa agtaccgcct gaataaggca taacaccgtt ccgacgggcc cggtccgaaa
     5161 cactcgcgaa ggctcagaat cgcgggcatc tccaacagtc cgcaaccaac cgctagaaaa
     5221 gcgcaaggca ccggcagcgg actttgtccc ggcctcgtag gatcgaggcc atgaaattgg
     5281 tccgcacttg ttttttccgg cgctgcagag cggttgcatg ccgcgctttt ttcgttacca
     5341 ctgcatcctt aatcgtcgcg caatcttctt cagctgatca gccagccgtg agcaacgcgg
     5401 agttgctaag gaaaatggaa gcgctcgaag cgaaggtcgc cgggcttgag agccggctgg
     5461 cagagtatga gaacggggga gagaaaaata aaagtgccgg ggcaaaattg aaggccaatc
     5521 catatgccga tgacgcctcg ggtcctcaag gccaacagag catgaccgca aggagcgaga
     5581 cagccgcttc ccctaacgat cgttcacttt ttggtctcgg ttaccgcagc caggcaatcc
     5641 tgagcatcgg agcctacggc gaattgaaat ttggcgggca gcagacgccg gaaggttgga
     5701 aagacggttt cgatgccggg cgcatcgtgc tcctgccgac gtatcaaatc gccgataaca
     5761 tcatcttcaa cgccgagatt gagttcgagc acggcggcat cgccgacgat gccgatgaca
     5821 agctgaccgg tgcggtggaa ctcgagcagg cctatatcga cttcaggttc aacgaacact
     5881 tcaactggcg tgcgcccggg gtcgatgtcg tgcctttcgg tttcatcaac cttttccatg
     5941 agccgacgca attttatagc gtggatcggc ctgagctcga taacggcctc attccgacca
     6001 cctggttcga aggcagtacc agcatctacg gcaaaatcgt ggacaacctt aattaccaat
     6061 tccagatcaa caccgggttg gaagatcttg gcgagaattg tggcgtgccc gagggacctt
     6121 atgaggccgg tatttctggc accgaagcgc tcgccctcgc ccggacaccg atcggcgatt
     6181 tcaatcaaac caaagacgct ttgggctata cccttcgact cagttacacg ccgccattta
     6241 tccccggtct ggctgggagc acgagtgtct tctttacgcc caacgtgacc ccgcgcggcg
     6301 cgcattccga cgatggccgc agcctcggaa acggcagcgt caccatgttt gataccgagg
     6361 tgcgttatcg cattccgaag accggattcg agttccgcgg agaattcgtc gacgtcttgc
     6421 tgggcagtcc cgtcaacctg cgggcgaaca acgatggtga tccggaaaac aatgtcggca
     6481 atagcatgtg gggcttttcg cttgaagcgg cttatcacat cgatctcggc gatcgcttcc
     6541 gcaatggctg ggagctggtg ccgttctatc gctataccta cgaaaatctg cagacaggtg
     6601 gttttgccgg gagcgacgag aacttgccga ccggccaggg gcgccgtcag tttcacacca
     6661 tcggccttgc tcttttcccc acgccacagg ttgtcttgaa aattgactat caattcgcgc
     6721 tcgataatgc gccggactcg ccgcgcgcgg atcatctgtt gggcgcggtc ggtttctttt
     6781 tctaggatcg cagttcgaag ccgctccgag atggaccaat ttccgcgctg gattttgccc
     6841 gccgcgatca gcgtcacggt gccgctgcct tgctacgcgg tgcaatacct gacggttccg
     6901 gccgcacaaa aactgtgctt ccctgattgc gatcgctttg tcgcggctca cgtcaaattt
     6961 acgccagcgc aaatccacgc aatcgaagca gccagcggac aaaaggagtt gcttcgcggc
     7021 caggaaattt ggcgagcgac gcgaagcggg aaactgcagg gcttctttat cgtcgattac
     7081 gttatcggca aacatctcgt gatcgactat gcagtcgcgc tttccccgtc cggggccgtc
     7141 agacagatcg agatcctcaa ttatcgcgag agctacggcg gtgagattca aaatcccgaa
     7201 tggcgcaaac agtttgtcgg gaaaacctcc cgctcgcatt tcgccttgaa cgacggcatc
     7261 accaacatca gcggcgcgac tctctccagc cggcacgtca ccgaaggggt gcgtcgagtc
     7321 cttgccacgt atgaaatctg tctcaagtga ggtgcggcgg gcacgcccgc tcctcggcac
     7381 gttcgtcgaa attacggcgc aagggagaga cgaaacgacc ttgcatcgcg cgatcaatga
     7441 cgctttcgcc gcggttcgtc aggtgcacga tttgatgagc tatcacgatc ccgagagcga
     7501 cgtttcccgc ttgaatcgcc atggctttgt ccaaaacgta accgtccatc catggacctg
     7561 gcaggtcttg caggcggctg gtgaattctc gcgccagagc ggcggcgtat ttgatattac
     7621 ggtcgctccc gaactcacca gacggaatta tcttcctcca tcaggttacg actcggatgc
     7681 gagttctacc tggcgagacg tttccctggg cgaaaacggc agaacgtttt tccgtcgacg
     7741 cgcgaccatc gatctcggcg gaattgcaaa aggcttcgca gtcgatcgcg ccgttgaagc
     7801 actcgtcgcg ggcggtgcgc agagcggaat agtaaatgcc ggcggggact tgcgcgtctt
     7861 tgggcccgag ccgcgtcttg tccacatccg gcacgcgcag gattcgcgag caatcggtgg
     7921 cgcaatcatg ttacgcgaaa gttcgctcgc cacctctgct ctttacggtc gtgaaggcga
     7981 ccacaggcag acgggtgcgc tcatcgatgg ccggacgggc tgtcccgccc tcggaccggt
     8041 cagcgtgagc gtttacgcgc ccgattgcat gacagcggat gcgctgacca aaattgtgct
     8101 ggcgttgcgc gacgacgctc ggccaatctt ggatcgatat cgggccggtg catttatttt
     8161 tgtcggcgat tcttcgtttt gtgaactccg cagcagacag gcgacataac ggtatcaggc
     8221 tgggccgtcg ccacgaatgg tcggtctacg tcgtactggc gattgtcttc agcagcggtg
     8281 tggtttgggc gtggctccat catttcgcac ggcgagcggg cgaatttggc gagagcgggc
     8341 acccggccga attgtggatg caaaaagtcc acggcgcgtc cgcgatgttc gctcttatcg
     8401 tgctcgggtc gttgcttgtc tttcacattc cgcgggcgtg gagggcgaag cgcaaccgcc
     8461 ccacgggtag ctcgctcttc gctctcttcg catttctagc cggcagcggc tacgcgcttt
     8521 actattcggg cggtgaatcc ctgcgcgcgt ggatgagctg gagccatctc tgggttggaa
     8581 tcgctctgcc ggtcttcgtc gccctgcatg tatggcgcgg gaaacgggcg actgcgccga
     8641 aatcccttga aaagtcagtc cccgggatgg cggcgagaaa caattttcgt taggttcatt
     8701 cctcgacctg cccgccgcga tctgaaacac tgaaccgggc tcctttctcg cctatcgatt
     8761 ttttggcctg cccaaggaac caaaactggg tcagtattcc agacagaatc gagccaacca
     8821 ccatgaaaac caccgaacca gccgtctacg ctggccgcga acgtgtacct ggcatcgttg
     8881 cgcttttgcg atcaatgagt tcatccgaag ttgcagagtc gatgacgcac gatcgacctt
     8941 cgccacaaaa tgcctttata ggatggggat tctggcgtag aaatggggaa actgatccat
     9001 ggacattaat tctctaatgg agcccgaacg atgacacagc cacaacccta cgtccccacc
     9061 gcacgcgagc agaaatttct cgagagc
//
LOCUS       DATMNS010000163         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4880239, whole genome shotgun sequence.
ACCESSION   DATMNS010000163 DATMNS010000000
VERSION     DATMNS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4880239"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1198)
                     /locus_tag="VNW28_05690"
     CDS             complement(89..1198)
                     /locus_tag="VNW28_05690"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXA09453.1"
                     /translation="MKLIDRFVGRELIVNVLFAIAVLSLVLVVGNIFRKLLPLLVNHD
                     VPIEFLITFIAYVLPFSLIFTIPWGLLTAVLLVFGRLSADNELIALRSNGVSVTRVCV
                     PLAVLAVISTVICLWLNVDVAPAAQEKLRSSIFDLATQNPIALFGGDQVIDQFPGRKI
                     YVGKKEGNQLENIIVFQMDERALPVRVTHARSGKLETDLPNKRLLLHLYNARYQERDA
                     KDPYDLRKIRDGISLAEGTLPISLEELYEKEKKRPTRSALSLAQLMEQLKSGAGRSDL
                     SATRTEINKRFSFPFACIAFALIGVPLGITTHRRETSVGFATGLIVAIFYFLFIIIAD
                     TMRGNAKIHPELLVWFPNLLFIFIGAYLFRRLSKH"
     gene            1574..>2073
                     /locus_tag="VNW28_05695"
     CDS             1574..>2073
                     /locus_tag="VNW28_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09454.1"
                     /translation="MGAALAGGAVLGKVASASGQNYNPAIGDGIPTPANVSDKRLAQA
                     FAIRVGAATKEALIPIPPHTTNGDEQRYSDKSGTCTKGILQDGIGLVNLAAFQTFRHA
                     INTGKFADWENVITGGPRTQNGPLGGRAFALEGSDDVQFGDAPSPANQINQVVVPPAP
                     ALAGQT"
BASE COUNT          472 a          577 c          640 g          384 t
ORIGIN      
        1 aagaactgtg catatccgga ggggcgcccc tggacgcgcg gaagcgcgtc cctccgagcg
       61 ttagtgacac ttcgcctcga gacggcgcct agtgtttgct cagccggcga aagaggtagg
      121 cgccgataaa gataaagagc aggttaggga accagacgag cagctcggga tggatcttgg
      181 cattgccccg catcgtgtcg gcgatgatga tgaagagaaa ataaaagatc gccacgatca
      241 ggccggtggc gaagccgacc gaggtctcgc gccgatgggt ggtgatgcct aacggcacgc
      301 cgatgagggc aaaggcaatg caggcaaacg gaaaggagaa acgtttgttg atctcggtcc
      361 gggttgcgct cagatcggag cgacccgccc ccgacttcaa ttgctccatc aactgcgcaa
      421 gagagagggc ggagcgcgtc ggtcgctttt tctccttctc gtaaagttcc tcgaggctga
      481 tcggcagggt cccctccgcc aggctgatgc cgtcgcggat tttgcgcagg tcgtagggat
      541 ctttcgcgtc acgctcctgg tagcgcgcat tgtaaaggtg caaaaggagt cgtttgttag
      601 gcaaatcggt ttccagcttg ccgctgcggg cgtgcgtcac ccgcaccggg agcgcgcgct
      661 cgtccatctg gaagacgatg atgttctcca gttggttgcc ttcttttttg ccgacgtaga
      721 ttttgcggcc ggggaattgg tcgatcacct ggtcgccgcc aaaaagcgca atcgggttct
      781 gcgtcgcgag atcgaagatg ctgctgcgca atttttcctg cgcggccggg gcgacatcga
      841 cgttcagcca caaacaaatg acggtgctga tcacggcgag gacggcgagc ggcacgcaga
      901 cgcgcgtcac gctgacgccg ttggagcgga gggcgatcaa ttcgttatcc gccgagagcc
      961 ggccgaagac gagcaggacg gcggtcagca agccccacgg gatggtgaag atgagcgaga
     1021 aaggcaggac gtaggcgatg aaggtgatga ggaactcgat cggcacgtcg tgattcacga
     1081 gaagcgggag gagtttgcga aaaatgttgc cgaccacgag gacgaggctg agcaccgcga
     1141 tcgcgaagag cacgttaacg atcagctcgc ggccgacgaa gcggtcgatc aatttcatgg
     1201 cagcctgttc cggcgtcgtt ccatcccgct cactccgccg cttgtcaatg gccgatcggg
     1261 tcttgggccg ccgttcttcg actcgctggc ggaaatgggc gtttttggcg gacgcgcgcg
     1321 cgaaaactgc ctcgtcagct cctcagaatc ttcagaaaac cgctgagaga aggaatttcc
     1381 agaaaaattc cagagaattg gattgacaca agaattgtaa ctgaaatata accggctccg
     1441 gttaattagg agtactaaca atatgaatca taacgacacg gttccttcca aatcaacaca
     1501 gccagagagt gacctgcccg cgaaggatac gacatccaat cacggaacca gtcggcgtaa
     1561 atttctaagc caggtgggcg cagcgctcgc cggcggcgcg gtgctcggca aagtcgcctc
     1621 tgcctccggc caaaattaca atcccgcgat tggggatggc attcccacac ccgccaatgt
     1681 gtcggataag agactcgccc aggccttcgc gattcgggtt ggcgcggcga cgaaagaagc
     1741 gctcatcccg attccgccgc ataccacgaa cggcgacgaa cagcgctatt ccgacaagtc
     1801 aggcacctgt accaaaggta ttctgcaaga tggaatcgga ctggtgaacc tggccgcttt
     1861 ccagactttc cggcacgcca tcaataccgg taaatttgca gactgggaaa atgtgatcac
     1921 gggcggcccg cgaacacaga atggcccgct tggaggacgc gccttcgcct tggaagggag
     1981 cgacgatgtg cagttcgggg atgcaccatc gccggccaat caaataaacc aggtcgtggt
     2041 cccgcccgca cccgcgctcg ccggtcaaac cta
//
LOCUS       DATMNS010000164         2594 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_944513, whole genome shotgun sequence.
ACCESSION   DATMNS010000164 DATMNS010000000
VERSION     DATMNS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2594)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2594)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2594
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944513"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1006)
                     /gene="gnd"
                     /locus_tag="VNW28_05700"
     CDS             complement(5..1006)
                     /gene="gnd"
                     /locus_tag="VNW28_05700"
                     /inference="COORDINATES: protein motif:HMM:NF007161.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HXA09455.1"
                     /translation="MQIGLVGLGRMGANMARRLMKGSHECVVYDRNAETVDDLEKKGA
                     AGASSLEEVVEKLNPPRAVWIMLPSGAPTEETVVALSKLLAAGDAIIDGGNSFYKDDV
                     RRSAMLKESGIDYLDAGTSGGVWGLERGYCMMIGGDQSAAQRLDPIFATLAPGPGNIP
                     KTPGREKMQSTAEQGYLYCGPSGAGHFVKMIHNGIEYGLMQAYAEGFDILLGANSKNL
                     PAGLSYDLNLADIAELWRRGSVISSWLLDLTATALAGDNKLSSYSGFVEDSGEGRWTI
                     QAALEEGISAEVLSVALYTRFRSRMEHTFAEKVLSAMRKGFGGHVEPPPAIKMKKGL"
     gene            complement(1022..1708)
                     /locus_tag="VNW28_05705"
     CDS             complement(1022..1708)
                     /locus_tag="VNW28_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXA09456.1"
                     /translation="MKPAMKVLVIDVGGTHVKFLATGQGAPREFDSGPALTPKRLITA
                     VRKLVVDWKYDAVSIGFPAPVLRNRPVREPWNLGKGWAGFDFEAAFKCPVKVVNDAAL
                     QALGSYKGGKMLFLGLGTGLGSAMIIDRIVEPMELGHLPYKKATFEDYVGIRGLKKYG
                     QNKWQSFVIDVVKRLVAALEPDDVVLGGGNVKKLKKLPAGCRAGDNDDAFLGGFRLWQ
                     KSITGARKGR"
     gene            1992..>2594
                     /locus_tag="VNW28_05710"
     CDS             1992..>2594
                     /locus_tag="VNW28_05710"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA09457.1"
                     /translation="MLTVSQVTKSFAGRILFEDVSLQVNRGDRIGLVGPNGAGKSTLF
                     TLILGEASPDSGSVSLEKSAVIGFLPQETAAAGEETVLELALATSPELVQARQLIKQH
                     ESGNGADDEAYHAALHVLDEHGGWELEPKAKRILAGLAFRESDFARPASALSGGWIMR
                     AHLARLLVMEPDLLLLDEPTNHLDLESLVWFQEYLKNYPGA"
BASE COUNT          539 a          805 c          692 g          558 t
ORIGIN      
        1 tgttctacag gcccttcttc atcttgatcg ccggcggagg ttcgacgtgg ccgccgaatc
       61 ctttgcgcat cgccgataaa actttctccg caaaggtgtg ttccatgcgc gaacgaaaac
      121 gggtgtagag agcaaccgag agaacctcgg cggagatacc ttcttccagc gccgcttgaa
      181 tagtccaccg gccttcgccc gaatcttcga caaagcccga ataactactc agtttgttat
      241 ccccagccaa agctgtcgca gtcagatcca gcaaccatga cgaaataaca ctcccgcgac
      301 gccaaagctc tgcgatatcc gcgaggttga gatcgtagga aagtccagcc ggcaggtttt
      361 tagagttagc tccaagcaag atatcgaatc cctcggcata ggcctgcatc aaaccatatt
      421 cgattccgtt atggatcatc ttgacaaagt ggccggcgcc ggacgggccg cagtaaaggt
      481 agccttgctc cgcggtgctc tgcatttttt cgcgaccagg cgtctttgga atgttgcccg
      541 gacccggcgc gagcgtggcg aagatcggat cgagccgctg ggcggcggat tgatcgccgc
      601 cgatcatcat gcaatagccg cgctccaagc cccagactcc gccgctcgtg ccggcgtcca
      661 aataatcgat gccgctctcc ttcagcatcg cgctcctgcg gacgtcgtct ttatagaacg
      721 agttaccgcc atcaatgatc gcgtcgcccg ccgcgaggag tttggaaagc gcgacgaccg
      781 tttcttccgt gggcgcaccg gatgggagca tgatccaaac cgcgcgcggg ggattgagct
      841 tttcgaccac ttcttcgagc gacgacgcgc ccgccgcgcc tttcttttcg aggtcgtcga
      901 cagtctccgc gttccgatcg taaacgacgc attcatgcga gcccttcatc aagcgtcgcg
      961 ccatgtttgc gcccattcgg ccaaggccaa ccagtccaat ttgcatatta gtctttcgag
     1021 ttcagcggcc ctttcgggcg ccagttattg atttctgcca taaacgaaat ccaccgagaa
     1081 atgcgtcatc attgtcaccg gcgcgacatc ccgcaggtaa cttctttagt ttctttacgt
     1141 tgcctcctcc gaggaccaca tcgtcgggct cgagggcagc caccagacgc ttcacgacat
     1201 cgatgacaaa gctctgccat ttattctggc cgtacttctt caatccccgg atcccgacgt
     1261 agtcttcgaa ggtggccttc ttgtaaggca ggtggcctag ctccatcggc tcgacgatgc
     1321 gatctatgat catcgcggac cccagcccgg ttccgagtcc caggaacagc atcttgccgc
     1381 ctttgtaact cccgagcgcc tgcagcgccg catcgttcac gaccttgacg ggacatttga
     1441 aggctgcttc gaaatcgaaa cccgcccatc cttttccaag attccatggc tcccgaaccg
     1501 gtctgttccg gaggaccggg gccggaaaac caatggaaac ggcgtcgtac ttccaatcca
     1561 cgaccagctt ccggactgct gtaatcagcc gctttggggt caaagccggc cccgaatcaa
     1621 actcccgagg ggcaccttgg ccggtcgcaa ggaatttgac gtgggtgccg cccacatcaa
     1681 ttaccagcac cttcatggca ggtttcatgg caccaggtgc tctctcagcg cgctgctact
     1741 cgaatagatt gtttcattga ttggatcgac aaaaccaggc aacgggatat ctgcgaagct
     1801 cacgttcgac gaacgtcgcc aataccctgg aagaattcca gcgcaatcag gtcaaaaaga
     1861 atttttcgag cgcctttgat tcgggccggg aacggaggag tcgtgggtgg acagttagat
     1921 ctgcattgaa cgcgaatgag acagagcgct tgcgatcgcg cccgtctcag gaacagttcc
     1981 cgcttccgcc aatgctcacc gtttcacaag tcaccaagtc tttcgccggg cgcatccttt
     2041 tcgaggatgt ctcgctccag gtgaatcgcg gcgatcgcat tggactcgtc gggcctaacg
     2101 gcgccggcaa atcaaccctc tttaccctca ttctcggcga agcctcgccg gattccggtt
     2161 cagtctcgct cgagaaaagc gcggtcattg gttttctccc gcaagaaacc gcggcggctg
     2221 gcgaagaaac ggtcctcgaa ctcgcgctcg cgacctcgcc tgagttggtg caggcgcgac
     2281 aactgatcaa gcaacatgag tcgggcaatg gcgcggacga cgaggcgtat cacgccgcgc
     2341 tccacgtcct cgacgagcac ggcgggtggg agcttgaacc caaagcgaaa cgcattctcg
     2401 ccgggctcgc ttttcgcgag agcgattttg cccggccggc ttccgcctta agcggcggct
     2461 ggatcatgcg cgcacatctc gcccggctcc ttgtcatgga gccggatctg cttctcctcg
     2521 acgaaccgac caaccacctc gacctcgagt cgttagtctg gtttcaggaa tatttgaaaa
     2581 attatccggg cgcg
//
LOCUS       DATMNS010000165         4249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4415307, whole genome shotgun sequence.
ACCESSION   DATMNS010000165 DATMNS010000000
VERSION     DATMNS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4415307"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..444)
                     /locus_tag="VNW28_05715"
     CDS             complement(25..444)
                     /locus_tag="VNW28_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09458.1"
                     /translation="MRNRLFAIKAKVSDSGAETFVFSAQKTMYGGRQIAQGDTIFVFA
                     SEHEGGPGLIASGVVTSAKVIAKKRGIARQTPRVSISIRRTALAKRRLGRCKLKLFSN
                     WNDGRPETELNFKFYRQATNKIAGISDEATAFLRGFF"
     gene            complement(598..2112)
                     /locus_tag="VNW28_05720"
     CDS             complement(598..2112)
                     /locus_tag="VNW28_05720"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXA09459.1"
                     /translation="MPKVLLACLISIAFPVFQALAQTTAGVVQANDTAQKLVASIHGQ
                     ALWSHLVDFQRIADENPGPNGHGNRDTGTPGYKASVDYVARLMREAGYHVTIQPYLYR
                     NVEIGEQAHFTAPDKSYVFKQDWFVARLSAGGTISAPVQPASGSGRGDTPEEWKSFVP
                     GRVALLRRGSCSYDLQVTNACAAGAAAVILYNHDGADFALEPAGPDDGSAFQVRLIRP
                     ATIPVVGMASFAVGAELRRQCEEGHAPLVRLDIPAEQKSGVDYNVIAESPFGDADHVV
                     VVDAHLDSIYGAGILDNASGSSTILEVALQMAKTPTRNRLRYIWFGGEEIGLLGSRYY
                     TENLSAAERQRIAFDVDVDVTATPNYDYLVADPAFAPNVQRFPPNVVPQSRVGNQFFA
                     DYFDTIGTPARAAWFGNEGTDSNSFSLIGIPNSGILTQQDCCKKPWEIEIWGGVPGNY
                     EGKIPSFNGGCVDFPGRWCDNLDNNNPSVFEVASKASASAIFQLANFQFARASP"
     gene            complement(2285..3607)
                     /locus_tag="VNW28_05725"
     CDS             complement(2285..3607)
                     /locus_tag="VNW28_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXA09460.1"
                     /translation="MGSKLTLIGSGLAGGLLAGYLGRRGYEIDLYERRADPRAGNTVG
                     GRSINLALSTRGIHALRQLGIADEVLQHAIPMRGRMIHDRSGDLHFSPYDRDPNKCIN
                     SIGRGALNTVVIEAAQRYPNVRVKFNHRCIDVDLNAPAAQLLDTATNQLVEARADAVI
                     GVDGAFSAVRGALQRLDRFEYSQSYLAHGYKELTIPPAADGSWPMEKNALHIWPRKSF
                     MMIALPNPDGSFTCTLFWEFDGPRSFATTTSDDDFRRFFHEEFPDAVPLMPALLDDFR
                     ENPTGSLVTIRCAPWHYEDKVALVGDAAHAVVPFYGQGMNAAFEDCVVFDECLAEFPN
                     DRKRAFAEYFARRKENADALADLAVHNFIEMRDKTAARSFRAKKKLDHTLEGLLRGLY
                     LPLYTMVSFTRVPYAEAARRARKQDAIVYACLIALALFVVALLCVLLG"
     gene            3731..4147
                     /locus_tag="VNW28_05730"
     CDS             3731..4147
                     /locus_tag="VNW28_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA09461.1"
                     /translation="MNPEPEPDQNERSGGTFALKAILLVDDDKQLASALQWILADANF
                     LVDVAFDGNEALLKVKAHDYDAVICDLKMPHLRGDAFYLQAREMRPSLADRFIFITGY
                     ATDPNIRQFLIENEVKFLVKPFPIKGLISCVQEMSR"
BASE COUNT          843 a         1245 c         1332 g          829 t
ORIGIN      
        1 gtgttcacgt cgaaataggc agccttagaa aaacccgcgg agaaacgccg tcgcttcatc
       61 cgagatgccg gcgatcttgt tcgttgcctg ccgatagaat ttgaaattga gctcggtctc
      121 gggtcggccg tcgttccaat tggaaaaaag tttgagcttg caccgtccca ggcgccgctt
      181 ggccagcgcg gtgcgcctga tggagatact cacgcgcggc gtttgccggg caatcccgcg
      241 tttctttgcg atcactttgg ctgaagtgac gacgccgctc gcgatcagac ctggcccgcc
      301 ctcatgctcg ctcgcgaaga cgaagatcgt atcgccttgg gcgatctgcc tgccaccata
      361 catcgtcttc tgcgcgctga acacgaacgt ctctgccccc gaatcgctca ctttggcttt
      421 gatcgcgaac aacctgttcc tcatcgcacc tcgttaattt ccatggccgt tgatggcacc
      481 aggcctaacg ataataagat gatccgccgc cggcgttgga agaaactggt gcgagcgaat
      541 gaatggaatg gactggcaag tcatgagatg gacgggaagc cccttgtttg ttgcttttca
      601 gggcgaagcg cgagcgaact ggaagtttgc taattgaaaa atcgcggagg cggaagcctt
      661 cgacgccacc tcgaagaccg acgggttgtt gttgtcgagg ttatcgcacc agcgccctgg
      721 gaaatcgacg catccgccat tgaagctggg aattttgcct tcgtaattgc ccggcacacc
      781 gccccagatt tcgatttccc acggtttttt gcagcaatct tgctgggtga gaatgccgga
      841 gttgggaatg ccaatgaggc tgaaggaatt cgagtctgtt ccctcgtttc cgaaccaggc
      901 cgcgcgcgct ggagtcccga tggtgtcgaa gtaatcggcg aaaaactggt taccaacccg
      961 cgactgagga accacgttgg gcggaaagcg ttgcacattt ggagcgaaag ccggatcggc
     1021 cacgaggtaa tcgtaattcg gcgtcgcggt cacatcgaca tcgacgtcga aggcaattcg
     1081 ctgccgctcg gccgcgctta aattctcggt gtaatagcgc gaaccgagca ggccaatttc
     1141 ttcgccccca aaccagatgt agcgcaggcg attgcgcgtc ggcgtctttg ccatctgcaa
     1201 ggccacttcc agaatcgtgg atgacccgga agcgttatcc aaaatgcccg cgccataaat
     1261 agagtcgagg tgcgcatcga cgaccacgac gtgatccgcg tcgccaaacg gcgactctgc
     1321 gatcacattg tagtccacgc cggatttttg ctccgccgga atatcgagcc gcaccagcgg
     1381 cgcgtgaccc tcctcgcatt gccggcgcag ttccgcaccg accgcaaacg aagccatgcc
     1441 gacgaccggg atggtcgccg gacgtatcag tcgcacctgg aaggccgagc catcatcggg
     1501 ccctgccggt tccaaggcga agtctgcgcc gtcatggttg tagagaatga ccgcggccgc
     1561 gcccgccgcg caggcattgg tcacctgcag gtcgtagctg cacgacccgc gtcgcaggag
     1621 cgcaacgcga ccaggcacga agcttttcca ttcctccggc gtatcgcctc tgcccgagcc
     1681 actcgcaggt tgcaccggcg cggaaatcgt cccgcccgcg gaaagccgcg ccacaaacca
     1741 gtcctgcttg aaaacgtagc tcttgtccgg cgcagtgaag tgcgcctgct cgccgatttc
     1801 gacgttgcga tagagatagg gctgaatcgt gacgtgatac ccggcctcgc gcatcaaccg
     1861 ggcaacatag tcgaccgaag ccttgtagcc cggcgtgccg gtatcgcgat tgccgtgacc
     1921 gttcgggccg ggattttcgt cggcgatgcg ctggaaatcg acgagatgac tccagagcgc
     1981 ctggccgtgg atggatgcga ccagcttctg cgccgtgtcg ttcgcctgga caacgcccgc
     2041 agtcgtctgc gcgagcgcct ggaaaactgg aaatgcgatg gaaattaagc aagccagcaa
     2101 gacctttggc aactgggcaa gcttcatagc gaatgcagca tcgcgctctt gcgacgcctg
     2161 taaagtggac ttcgcgcgcc tccatggggt aaagtcattt catcattccg aagtgcgcgc
     2221 ctaaccagca caagatgagc cgccgcgagc gaatggcgcc tctggagaaa gagcgcccgg
     2281 tgcgctagcc taacaggacg cacaaaagcg cgacgacaaa gagcgccagg gcgatcaggc
     2341 aggcgtagac aatcgcgtcc tgctttcgcg cccggcgagc ggcctcggcg taggggacac
     2401 gggtgaaact caccatggtg tagagcggga ggtagaggcc gcggagcagg ccttcgaggg
     2461 tgtggtcgag ttttttcttc gcccggaagc tgcgcgcggc ggtcttgtcg cgcatctcga
     2521 tgaagttgtg tacggcaagg tcggcgaggg cgtcggcatt ctctttgcgc cgcgcgaaat
     2581 actcggcgaa ggcgcgctta cggtcgttag ggaactccgc caggcattcg tcaaagacga
     2641 cgcaatcctc gaaggcggcg ttcatcccct gcccgtagaa aggtacgacg gcgtgggccg
     2701 cgtcgccgac gagtgcgacc ttgtcctcgt aatgccacgg cgcgcagcgg atggtgacga
     2761 gcgagccggt cgggttttcc ctaaagtcgt cgagcagcgc gggcatgagc gggacggcgt
     2821 cggggaattc ctcgtggaag aagcgtcgga aatcgtcgtc gctcgtcgtg gtcgcgaagc
     2881 tgcgcgggcc gtcgaattcc caaaagagag tgcaggtgaa ggaaccgtcg gggttgggga
     2941 gcgcgatcat catgaagctc ttgcgcggcc agatgtggag agcgtttttt tccatcggcc
     3001 acgagccgtc agccgcgggt gggatggtga gttccttgta accgtgggcg agatagctct
     3061 ggctgtattc gaaacggtcg agccgttgca gcgcgccgcg gaccgcggag aaagcgccat
     3121 cgactccgat aacagcgtcg gcccgcgcct cgaccaactg gttcgtcgcg gtgtcgagca
     3181 gttgcgcggc gggcgcgttc aggtcgacat caatacagcg gtgattgaat ttgacccgca
     3241 cgttgggata acgctgcgcg gcttcgatta cgacggtatt gagcgcgccg cggccgatcg
     3301 agttgatgca tttgttcggg tcgcggtcgt agggtgagaa atgcaggtcg ccggagcggt
     3361 catggatcat gcggccgcgc atcgggatgg cgtgctggag gacttcatcg gcgatgccga
     3421 gctgccggag ggcgtggatg ccgcgggtgg agagagcgag attgatcgag cgcccgccca
     3481 ctgtgttgcc cgcacgcggg tcggcccggc gctcgtagag atcgatttca tagccgcgcc
     3541 ggccgaggta accggcgagt agtcccccgg cgagcccact gccgatcagg gtcaattttg
     3601 atcccattgg gctctaccgt ggccgtttcc ggtaatttgc caagcccgga aggcgcgcgt
     3661 cgattggaga tcgggcggtt gcaaataccg cgcgtggcac tatggctgct caaggtatgc
     3721 cttcagcgca atgaatcctg aacccgaacc agaccagaac gagcgcagcg gcgggacttt
     3781 tgcgctcaaa gcgatcctcc tggtggacga cgacaagcag ctcgcctcgg ccctgcagtg
     3841 gatcctggcg gacgcaaatt ttctcgtcga tgtggccttc gacggcaacg aagcgttgct
     3901 caaagtgaag gcgcacgatt acgacgcggt catctgcgac ctgaaaatgc cgcacctgcg
     3961 gggcgacgcg ttctatctcc aggcgaggga aatgcgcccg tctctagcgg atcgtttcat
     4021 tttcatcacc ggctacgcga ccgaccccaa catcaggcaa ttcctgatcg agaacgaggt
     4081 caaattccta gtcaaacctt tccccatcaa aggactgatt tcctgcgtgc aggaaatgtc
     4141 ccgctaagca tttcgcggcc ttcgtcggcg atcgttgtct tcgttagggc agttgaccgg
     4201 gaccccgtcc ttcagaccct ggcgtcgatc cactccgtcg ccggaagtc
//
LOCUS       DATMNS010000166         2991 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_948653, whole genome shotgun sequence.
ACCESSION   DATMNS010000166 DATMNS010000000
VERSION     DATMNS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948653"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..439)
                     /locus_tag="VNW28_05735"
     CDS             complement(266..439)
                     /locus_tag="VNW28_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09462.1"
                     /translation="MIKRILVRCPTTKKLNVTGIVIEEDVFAATKLKPGMVTCSHCGG
                     KHAWDKKQVILAR"
     gene            847..1638
                     /locus_tag="VNW28_05740"
     CDS             847..1638
                     /locus_tag="VNW28_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04282 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXA09463.1"
                     /translation="MSAAYRVISPHDPDPTAAGCCALAIMTKAPRAGQVKTRLTPPLT
                     ATEAAALNICFLRDISAAIARVGANTSGIGCYTPVGAEETYRGIFPDKFQLIAQREGN
                     FGDRLIFAVQDLLGVGFASVCLINSDSPTVPTSAFVQAAEILSSPGDSVVLGPSDDGG
                     YYLIGLKQQHRRLFAGIDWSTDRVCAQTLQRAAELHLDVRLLPPGYDVDDCATLRRLC
                     DDLLGPNASGNKNTAPATREFLAEIIACEGRARLWPIEPVTLSAR"
     gene            1635..2756
                     /locus_tag="VNW28_05745"
     CDS             1635..2756
                     /locus_tag="VNW28_05745"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXA09464.1"
                     /translation="MKRALVTGGAGLIGSHLADLLRREGWRVRVLDNLEPQTHRHGRP
                     AWVVEDADFIEGDLRDRATMTAALAGVDVVFHQAAYGGYMPEIAKYVHVNSLGTAQML
                     EVIREHNLPIRKIVVASSQAVYSEGAGRCPEHGLVFPLVRPVEQLRTGDWQVHCPVCG
                     AVTTTAPTPENAPIGGETVYGLTKVDQEKLVLLWGKQVGIPTVALRYSCTYGPRQSIF
                     NPYTGVIAIFCTRLLNNLPPVLYEDGEQTRDFSFVEDIARANLLAAESDSLDGLPVNV
                     GSGRGVPIREVAEQISHALGIRIAPQAEGEFRPGEMRHLTSDIERIRAAGYQPQVDLA
                     AGIGRYLDWIRGQGDVRDYFSDAADVLRRKGIVHRVARA"
     gene            2753..>2991
                     /locus_tag="VNW28_05750"
     CDS             2753..>2991
                     /locus_tag="VNW28_05750"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA09465.1"
                     /translation="MKSRISVIIPALDEEGPIGAVVRACLTTGLPGEVIVVDNGSTDA
                     TCERAQAAGARVVSAPRGYGRACAAGIAALDSQTE"
BASE COUNT          564 a          940 c          881 g          606 t
ORIGIN      
        1 cgtcatgaat cttgcggacc cgctccggct cgctgacgca gagttcctcg aaacaacgct
       61 cgaggggaac gccgtcgtcg agcagcatcg tgcccatcgc gccgtcgccg cagactaaac
      121 ggctctgaag ttcgtcaagg aggtccatcg gctaagcgct tagcgccgac ccgggcggag
      181 gtcaatccgt catccccacg gcacagagca gaacgactgc cccccatcgg cttcgtcgaa
      241 atcctgccca ctgaagcact cgagcctaac gagcgagaat aacctgtttc ttatcccagg
      301 catgcttgcc tccgcaatgc gagcaggtca ccatcccggg cttgagtttg gtggcggcga
      361 acacatcctc ttcaatcacg attccggtca cgttgagttt tttcgttgtc ggacatctca
      421 ccagaattct tttgatcaca gcgcgaatat ctcacacgcg acgccgaaat gctcgcgcta
      481 tcttccttgg tgttccaacc gcttcctcat catcctcgtg ttaaaaggat tgctcaagaa
      541 agagccaaca acttccaaca aacaccaata agtgatggcc atctgtccaa ctcttgtttt
      601 gtcggtcttg gcgttgcact gttattaacc gccaccaaac cgattttccg cggggaaata
      661 gcgcgccaga cggggttatt aagacaatga agattaagga tttgaatcgt tcctacaagg
      721 aatcctcttc agagctgtgc aaaactttgg cgctcacatc tccgcagata gctttcgcct
      781 ggatgaaatg attgccgcgg agcaatctct caaacggtgt atgaaccgcg ctctttgcga
      841 aaaggcatga gcgccgcgta ccgcgtaatc tcaccgcacg accctgatcc cacggctgca
      901 ggctgctgcg cgctggcgat catgacgaaa gcgccgcggg ccggccaggt gaagacgcgc
      961 ctcacgccgc ccctcacggc gacggaagca gccgcgctga acatttgctt cctgcgcgat
     1021 atctccgccg caattgcgcg ggtgggcgcg aacacgagcg ggattggctg ctacacgccc
     1081 gtcggcgccg aggaaacata tcgcgggatc tttccggaca aatttcaact catcgcccag
     1141 cgcgagggta attttggtga tcgactcatt tttgccgtgc aggatttgct cggtgttggc
     1201 tttgcttcgg tctgcctgat caactccgac agtccgactg tgcccacgtc tgcttttgtg
     1261 caggcggcag agattctttc ctcgcctggc gacagcgtcg tgctcggccc atccgacgac
     1321 ggaggttact atctgattgg cttaaagcag cagcatcgtc ggctcttcgc cgggattgat
     1381 tggagtaccg atcgggtctg cgcgcagacg ttgcagcgtg ccgcggagtt gcaccttgac
     1441 gttcgtcttc tgccgccggg ttacgacgtg gatgattgcg cgacgctgcg ccggctttgc
     1501 gacgatttgt taggccctaa tgccagcggg aacaaaaaca cagcgcccgc gacgcgcgaa
     1561 tttctcgcgg aaatcatcgc gtgcgagggc cgcgcgcgtc tctggccgat cgagccggtc
     1621 accctttccg cgcgatgaaa cgtgcgctcg tcaccggcgg cgcgggtttg atcggttccc
     1681 atctcgccga cctcctccgg cgcgagggct ggcgcgtccg cgtcctcgat aatctcgaac
     1741 cgcaaaccca ccgccacggc cggccggcct gggtggtgga ggatgccgat ttcatcgagg
     1801 gcgatctccg cgatcgcgca acgatgaccg cggcactcgc cggcgtcgat gtcgtctttc
     1861 atcaagcggc ctacggcggt tacatgccgg agattgccaa gtatgtgcac gttaacagcc
     1921 tcggcaccgc gcaaatgctc gaggtgattc gagaacacaa cctgccaatc aggaaaatcg
     1981 tcgtcgcgtc atcgcaggcg gtttacagcg agggcgccgg ccgttgcccg gaacatgggc
     2041 tcgtctttcc gctggtgcgc ccggttgaac aactgcggac gggcgattgg caggtgcatt
     2101 gtccggtttg cggggcggtc acaacgactg cgcccacgcc ggagaacgcc ccgatcggtg
     2161 gcgagacggt ttacggcctg acgaaggtcg atcaggagaa attggtgctc ctctggggca
     2221 aacaggtcgg catcccgacc gtcgcgctcc gctattcctg cacctatggc ccgcgccaat
     2281 ccatcttcaa tccctacacc ggcgtgatcg cgattttctg cacccgcttg ctcaataact
     2341 tgccgcctgt gctctacgag gatggcgaac agacgcgtga tttttctttc gtcgaagaca
     2401 tcgcgcgcgc caacttgctc gccgccgaaa gcgactcgct cgacggcctg ccagtcaacg
     2461 tcggcagcgg ccgcggcgtc ccaattcggg aagtggccga acaaatttct cacgcgctcg
     2521 gtattcggat tgcgccgcag gcggaaggcg aatttcgacc cggcgagatg cgtcacctca
     2581 ccagtgacat cgagcgcatc cgtgcggccg gctaccagcc gcaggtcgat ctcgccgccg
     2641 ggattggtcg ctatctcgac tggattcgcg ggcaaggaga cgtgcgcgat tatttcagcg
     2701 acgcggctga tgtcctgcgc cgcaagggta tcgtccaccg cgttgcccgg gcgtgaaatc
     2761 gcgcatctcc gtcatcattc ccgcgctcga cgaggaaggg ccgatcggcg cggtggtgcg
     2821 cgcttgccta acgaccggtc ttcccgggga agtcatcgtg gttgacaacg gcagcaccga
     2881 cgccacgtgt gagcgcgccc aggcagcggg cgcgcgggtc gtgtccgcgc cgcgcggtta
     2941 cgggcgcgcc tgcgcggcgg ggatcgcggc gctcgattca caaaccgaaa t
//
LOCUS       DATMNS010000167         9491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4417185, whole genome shotgun sequence.
ACCESSION   DATMNS010000167 DATMNS010000000
VERSION     DATMNS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9491
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4417185"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..2225
                     /locus_tag="VNW28_05755"
     CDS             180..2225
                     /locus_tag="VNW28_05755"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HXA09466.1"
                     /translation="MKRARSRGLPRRFALTTLVCFCSRDASHREFFMMLFRMLRVGVL
                     TGLLSLWLVPLASGQLAPTQRVPRNVPLETYDNPPAAPRVMETSPRMISQFGSFTSFQ
                     VNVNASGQNILGDAANECSISVNPIDGTKKTIAWRQFNDVTSNFRQGGWGYTTNGGTT
                     WTFPGVLENGVFRSDPVTKSDEVGNFFYLSLQSDVNEDFFCDDLWRSLNGGQSWTLLS
                     GEKGGGGGDKQWLTIDKTSGPGHGFQYQSDDGINCSGGGVQFQRSINGGVTWQSPIVI
                     PNSPTDGTLDVDSNGNLFIGGEGFSQFFCERSSNAQLASQTPTFDQSKSVNLGGFLGS
                     GGINPAGLDGMVFLAIDHSGGPTNNNIYMLASVVPSGRSTTDVMFVRSTDGGLTFSTP
                     KKINDDPVNPSKWHWFGTFSVAPNGRLDAVWYDTRNAANNTDSQLFYSWSTDAGVTWA
                     PNVAVSIAFNPFEGYPNQSKIGDYITMVSDSTGADVAYSATFNFNASRNQHEEDVYYV
                     RVAPTASGINLLSAASRLTHGAAGTFDVNMPLTGVSGVECRSATTYNAVFTFDAPVTS
                     GQVTVLSGTATVGAITFSGNSMTAQLTGVTAAEVVTLHTQNINGDGLPHGDVPFGFLV
                     ADADASRVVGKSDQTLVKGQLNQPVTSANFREDLNADGRIKSADTNLVGTNKGHSIP"
     gene            2471..3070
                     /gene="pgsA"
                     /locus_tag="VNW28_05760"
     CDS             2471..3070
                     /gene="pgsA"
                     /locus_tag="VNW28_05760"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HXA09467.1"
                     /translation="MTTANKITVVRILMIPAFVMMAIYYGESIKAGAPQEWQRFTAIA
                     IFLVAAVSDGLDGYIARRYNQKSALGVILDPIADKGLLLSGIITLSISNWSDVDPQYG
                     RFPVWFPVLVISRDVVIVVGSVVLQLLVGKVRVQPNWTGKIATFLQMVAIAWVMLQLM
                     FIPLLYIVVAAGFFTFVSGIVYVTDGFRQLGAHGQSDAS"
     gene            3088..4521
                     /gene="der"
                     /locus_tag="VNW28_05765"
     CDS             3088..4521
                     /gene="der"
                     /locus_tag="VNW28_05765"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HXA09468.1"
                     /translation="MEKARTRNVAIVGRPNVGKSALFNRLAGRSIAIVHDQPGITRDR
                     ISALSRRGTEPFTIWDTGGIGGAGETELLKEVRSAADAAMREAQVILFLVDAQQGLTP
                     IDQELARLLRRSRRPVVLVVNKIDHPKHEDLAGEFTRLGFPDVVSISAAHGLGIGSLV
                     ETIERLLPPITNNQEPITAALSLAIVGRPNVGKSSLINAILSDRRTIVSNLPGTTRDA
                     VDIAYRHGADDFVLIDTAGMRARGKHSTSVEVFSVMRSERTIRRADLCVLVIDATSGV
                     TAQDKKIAALMQKAHKPSLLLVNKWDLVKPERGVREALAKLIEETRERLFFLPYAPIL
                     VGSALTGENIDRFFGAIRKIRRAADGRLSTGVLNRLLREAFAANPPPMSGNKRMKLFY
                     ATQARGKTERALATPEFVLFVNDPQLLGETYARYLEARLREAQPFPGLPLMLNFRPRS
                     ETPGERTACPPGAGDSRAVRGDPPRIR"
     gene            complement(4672..5805)
                     /locus_tag="VNW28_05770"
     CDS             complement(4672..5805)
                     /locus_tag="VNW28_05770"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXA09469.1"
                     /translation="MTARPTLRVGLIGHGFMGKAHSNAWRQAPRFFDLPAEVRLSTLC
                     GRDARAAKKAAVDFGWEKSATDWRAVVADPGIDLIDISTPNDSHAEIAIAAAAAGKAI
                     LCEKPLARNVAEAERMTRAVRQARVVNMVCHNYRRVPAVALAREMIQRGELGERLFHF
                     RARYAQDWIVDPEFPLVWRLRSGPAGSGSLGDLGSHLVDLGRYLVGEFREVCATLETF
                     VKRRPVSAARGRRGKVTVDDAVAVIGRFRNGALATLEATRFAPGRKNGLTIEINGSEG
                     SLAFDLEAMNRLQVYSARDPATQRGFREIIVTEDAHPYVKHWWPPGHLLGYEHSFVHT
                     IADFISAVVARKNAPPTFADGLATQRVLEAIAQSARRRAWVTL"
     gene            complement(5952..6923)
                     /locus_tag="VNW28_05775"
     CDS             complement(5952..6923)
                     /locus_tag="VNW28_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09470.1"
                     /translation="MAFTPKVALDYLREAQARSRLGHAYLITGATGSGKRAFAADVAH
                     LVNGASPGAVFAGSAPDVYLASPESKSRRIVIEQIRALEHALQMRASDGRRKVAIISE
                     ADRMIEQAANAFLKTLEEPPNNSLLLLLSAIPEVLPDTIRSRCLAVPLTPPTDAKLSD
                     EENELVELLNGHAAVGSGDVHQAYRLVQGFQRLLGGIRRRIEGENDEAQQREETRYRN
                     TTDGAWLERREEHYKALTESLYLHRRAELVEILFRWWGDVLRSSTGVPARELPTARAS
                     TEASARRLTTPEILRRLRRLEELRDHLGRNIQEALALEVAFLNIFRF"
     gene            complement(6926..7609)
                     /gene="tmk"
                     /locus_tag="VNW28_05780"
     CDS             complement(6926..7609)
                     /gene="tmk"
                     /locus_tag="VNW28_05780"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HXA09471.1"
                     /translation="MPRLPFITFEGSEGCGKSTQVGLLAARLTNAGVPLLVTREPGGT
                     PIGERIRHLLQFAPESVAMTPETELLLFEASRSQLVREVIQPALDRGTVVLSDRFADS
                     TTVYQGVARRLDHEMIGHLNAFAVGNCWPDLTFVLDTDAETARTRMMRRVRPAAGIDR
                     MEQEPLKFYEAVCAAYRELAAREPQRIRLIDATRTVEELEEEIWEIIATRFGHLSPLL
                     PLLRQRRRS"
     gene            complement(7984..9054)
                     /locus_tag="VNW28_05785"
     CDS             complement(7984..9054)
                     /locus_tag="VNW28_05785"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXA09472.1"
                     /translation="MRLRIAVLIWSVALTAALQAADYPAPHEGDFIARDFKFTSGATL
                     PELRLHYRTLGKPAKDAHGVTQNAVLIMHGTTGSGAQFIRPEFAGELFGPGQPLDAAK
                     FFIVLPDDIGHGKSSKPSDGMHAKFPNYGYRDMVEAEYRLLTDGLGVDHTRLIMGTSM
                     GGMHTWLWGETHPEFMDALMPLASLPTQISGRNRAWRRIVIDAIRNDPAWHGGDYQTQ
                     PPSLRTAAEMLWFMSSNPILRQKEAPTLARTDEVLDKFGNDDVKTSDANDVLYAVEAS
                     RDYDPGPNLEKIRAPLLAINSADDLINPPELGILEREIKRVPKGRAIVIPLSDKTRGH
                     GTHTMAALWKDQLVELLKRSEH"
     gene            9288..>9491
                     /locus_tag="VNW28_05790"
     CDS             9288..>9491
                     /locus_tag="VNW28_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXA09473.1"
                     /translation="MKKYWLVKQEPEAYSWNDFVRDGLTKWTGVRNFQARNNLRAMRK
                     GDAVLYYHSVSEKAAVGIAQVTRE"
BASE COUNT         1766 a         2945 c         2940 g         1840 t
ORIGIN      
        1 acgcccgtca cgatcaccgg cacttcgggc agcctgacca agacttttaa tttcacccta
       61 acggtgaatc catccgcaaa ttaacgcagc cgtcacgctg ctcgtcgcat cgcaaagtgg
      121 cactgaccgg cttcgccgtc cgaagaagct gacaaaaaag taaggttggc ggctcacgaa
      181 tgaaacgcgc tcgctcgcgg gggctgcctc gccgttttgc cttgactacc ctcgtctgtt
      241 tctgtagtcg ggacgcttcg catagggagt tttttatgat gctttttaga atgttaagag
      301 tcggcgtttt aaccggtctg ctatcgctgt ggttggttcc cctcgcgtcg ggccaactgg
      361 cgccgaccca gagggtgcca cgaaatgtgc cgctggagac atacgacaac ccgccggctg
      421 cccctcgcgt catggagact tcgccgcgca tgatctcgca attcggttcc ttcaccagct
      481 tccaggtgaa cgtcaatgcg agtggccaga atatcctcgg cgatgccgcg aatgagtgct
      541 cgatctcggt gaatcccata gacggcacca agaaaacgat tgcctggcga caattcaacg
      601 atgtgacctc caacttccgg cagggtgggt ggggttacac gaccaatggc gggaccacct
      661 ggaccttccc cggggttctc gaaaacggcg tcttccgcag cgacccggtg acaaagtccg
      721 atgaggtcgg aaacttcttc tatctcagcc tgcaatccga cgtaaacgag gatttttttt
      781 gtgacgatct gtggcgctcg ctcaacggcg gtcaatcctg gacgctgctt tctggcgaga
      841 aagggggcgg cggcggcgac aagcaatggc tcacgatcga taagaccagc ggcccgggcc
      901 atgggtttca ataccagtcg gacgacggca ttaactgcag cggcggcggg gtgcaatttc
      961 aacgttccat caacggtggc gtcacctggc agtcgccgat tgttattcct aactcgccga
     1021 cggacggaac gctggacgtg gacagcaatg gcaacctgtt tatcggcggc gaaggattca
     1081 gccagttctt ctgcgagcgc tcgagcaacg cgcagcttgc gagccagaca cctacttttg
     1141 accaaagcaa atccgtcaac ctgggcggct tcctcgggag cggtgggatc aatccagccg
     1201 gactggatgg aatggtattc ctcgccatcg atcactccgg cggaccgacc aacaataaca
     1261 tttacatgct ggccagcgta gtgccgtccg gccgcagtac gaccgacgtg atgtttgtcc
     1321 gcagcacgga cggcggcctg accttcagca cgccgaagaa gatcaacgac gacccggtca
     1381 atcccagcaa gtggcactgg tttgggacct tttcggtcgc tcctaacgga cggctcgatg
     1441 cggtctggta tgacacgcgg aatgcggcga ataacaccga ttcgcaactc ttctattcgt
     1501 ggagcaccga cgcgggcgtg acctgggcgc cgaacgtggc ggtgagcatt gcgtttaatc
     1561 cgttcgaagg ctatccgaac cagagcaaga taggcgatta catcaccatg gtctcggaca
     1621 gcaccggcgc cgatgtggcc tattcggcta cctttaattt caacgcgagc cgaaaccagc
     1681 acgaggaaga cgtttattat gtccgggtcg ctcctactgc ctctggaatc aacctcctga
     1741 gcgcggcgtc gcgcctgact catggcgcgg cggggacatt cgatgtgaac atgccgctaa
     1801 ctggtgtctc cggggtggaa tgtcgcagtg ccacgaccta caacgccgtc tttaccttcg
     1861 atgccccggt cacgtcgggt caggttactg tcctaagcgg cacggccaca gtaggagcca
     1921 ttaccttcag tggcaacagc atgacggcac aactcacggg tgtgacggcg gccgaggttg
     1981 taactctcca tacgcagaac atcaatggcg atgggctacc gcatggagat gttccatttg
     2041 gtttccttgt cgccgacgcc gacgccagcc gtgtggttgg caagtccgat cagaccctgg
     2101 tcaagggcca actgaaccag ccggtgacga gcgccaattt ccgcgaggat ctcaacgcag
     2161 acggccggat caagagtgcg gacaccaatc tggtggggac taacaagggc cactcgattc
     2221 cctaactcga gcgagactac ctgattggag ggaatcgcgt tgtcgattcc gcggtcccga
     2281 cggcgcggtg ccctccaagg gatcttccga tcgtcattcc ggatgggaat aggcgccgga
     2341 tgcgtcgttt ggaggccaaa atgaggggtg gaacgcgaag tgtcggacac ggcgtcgggc
     2401 ccaaatcggt cggggtgcac ttgtcccctt tcttttttcg ccaagccgtg ggcatattgc
     2461 cttcgtcctc atgaccaccg ccaataagat caccgtcgtc cgcatcctga tgatccccgc
     2521 cttcgttatg atggcgatct actacggcga aagcatcaag gcgggcgcac cgcaggaatg
     2581 gcagcgcttt acggcgatcg cgatctttct cgtcgccgcg gtgagcgatg gtctggatgg
     2641 ctacatcgcc cgccgctata accaaaagag cgcgctcggc gtgattctcg atccgatcgc
     2701 cgacaagggt ctgctcctca gcggcatcat tacgctgagc atcagcaact ggagcgacgt
     2761 tgatccgcaa tacggacgtt tcccggtctg gtttccggtc cttgtcatta gccgcgatgt
     2821 cgtcatcgtc gtcggcagtg tggtgctcca gttgctggtc ggcaaagtgc gcgtgcaacc
     2881 gaactggaca ggaaaaatcg caaccttcct ccagatggtg gcgatcgcgt gggtcatgtt
     2941 gcaactgatg tttatcccgt tgctttacat cgtggtggcc gccggatttt tcacttttgt
     3001 ctctggcatc gtctatgtga cggatgggtt ccgccagctc ggcgcccacg gccaatcgga
     3061 cgccagctag ccggcgcggg ggatttcatg gaaaaagcac ggacgcgcaa tgtcgccatc
     3121 gtcggacggc ctaacgttgg caaatccgcg ctcttcaatc gcctcgccgg ccgtagcatc
     3181 gcgatcgtgc acgaccagcc cgggatcacg cgcgatcgta tctctgccct cagccggcgc
     3241 gggacggagc cgtttaccat ctgggatacc ggcggaatcg gcggggcggg ggaaaccgag
     3301 ctgctgaagg aagtgcgcag tgcggccgat gcggcgatgc gggaggcgca ggtcattctc
     3361 ttcctcgtcg acgcgcagca aggtctcacc ccgatcgacc aggaactggc ccgcctcctg
     3421 cgccgctcgc ggcgtccggt cgtgctggtg gtaaacaaaa tcgatcatcc caaacatgag
     3481 gatctggccg gagaattcac ccggctcggt ttcccggatg ttgtcagcat cagcgccgcg
     3541 cacggtttgg ggattggctc gttagtcgag acgatcgaac ggctgcttcc tccaataacc
     3601 aataaccagg aaccaataac cgccgccttg tccctcgcca tcgttggccg gcctaacgtc
     3661 ggcaagtctt ccctgatcaa cgcgatcctg agcgaccggc gcacgatcgt gagcaacctg
     3721 cccggcacga cgcgcgacgc cgtcgacatc gcctatcgac atggcgcgga tgactttgtc
     3781 ctgatcgata ccgcaggcat gcgcgcgcgg ggcaaacatt ccacttccgt ggaagtcttc
     3841 agcgtgatgc gctccgagcg cacgattcgc cgggccgatc tttgcgtgct cgtgatcgac
     3901 gccaccagcg gggtgactgc gcaggacaag aaaattgccg cgctgatgca gaaggcgcac
     3961 aagccctcgc tcctcctcgt caacaaatgg gatctcgtga aaccggagcg cggcgtcagg
     4021 gaggcgctcg cgaagctgat cgaggaaacg cgcgaacgcc ttttctttct gccctacgcg
     4081 cccattctcg tcggctccgc gctgaccggc gagaatatcg accgcttctt tggcgcgatc
     4141 cgcaagatcc gccgggcggc ggacgggcgg ttgagcaccg gcgttctgaa ccgactcctg
     4201 cgggaagcgt ttgccgccaa cccgccgccg atgtccggca acaagcgcat gaagttgttt
     4261 tacgccaccc aggcgcgcgg caagacggag cgcgcgctcg cgactccgga gttcgtcctt
     4321 tttgtcaacg atccgcaatt gttaggcgaa acctacgcgc gttatttgga agcgcgcctc
     4381 cgcgaagcgc agccgtttcc cgggctgccg ctgatgttga atttccggcc gcggagcgaa
     4441 actccggggg agcgcactgc ctgcccgccg ggtgcgggcg actcgcgtgc cgttcgcggc
     4501 gatccgccgc gaattcgtta ggccacactg tggaggcgcg tgcgggcgcg tgcttgtggg
     4561 gaggtcggtt ccttccctcg aaccttcggc cgttcggcga gtcgccgaac ggggcacgcg
     4621 aggtgtgatg ctgcggacag agcaactctc gcgcttccca cagggaagcg gctacaacgt
     4681 cacccaggcg cgacgccgcg cgctttgcgc gatcgcttcg agcacgcgtt gcgtggcgag
     4741 accatccgca aacgttggcg gtgcattttt ccgggcgacg actgcgctga tgaaatccgc
     4801 gatcgtgtgc acgaaactgt gctcgtaacc gaggagatga ccgggcggcc accaatgttt
     4861 gacgtagggg tgcgcgtctt ccgtcacgat gatctcgcga aacccgcgct gcgtcgccgg
     4921 gtcgcgcgcg ctgtaaacct gcagccgatt catcgcctcg aggtcgaagg cgagcgagcc
     4981 ctcgctgccg ttgatttcga tcgtcaggcc gtttttccgt ccgggcgcga accgcgtcgc
     5041 ctcgagcgtg gcgagggcgc cgttgcgaaa tcggccgatg acagcgacgg catcatcgac
     5101 cgtcactttt ccgcggcgtc ctcgcgctgc cgagacggga cgccttttca caaaggtttc
     5161 gagggtggcg cagacctccc ggaattcacc caccagatag cgaccgagat cgacgaggtg
     5221 cgagccgagg tcgcctaacg atcccgatcc ggccgggccg gagcgcaacc gccagacgag
     5281 cggaaattcc ggatcgacga tccaatcctg cgcgtagcgg gcgcgaaaat gaaaaagccg
     5341 ttcgcccagt tctccgcgct ggatcatctc ccgcgcgaga gcgaccgccg gcacccgacg
     5401 ataattgtga caaaccatgt tcacgactcg cgcctggcgc accgccctgg tcatccgctc
     5461 ggcctccgcc acattccgcg cgagcggttt ttcgcagagg atcgccttgc ccgcggccgc
     5521 cgccgcgatc gcgatctcgg cgtgcgagtc gttaggcgtg ctgatatcga taaggtcgat
     5581 cccgggatcg gcgaccaccg cacgccagtc ggtcgcgctc ttctcccagc caaaatcgac
     5641 cgccgccttt ttggccgcgc gcgcgtcgcg tccgcagagc gtgctcagcc gcacctccgc
     5701 cggcagatcg aaaaagcgcg gcgcctgccg ccaggcgttg gagtgcgcct tgcccatgaa
     5761 tccatgacca atcagaccaa cgcgaagtgt cgggcgcgca gtcatgctct ggccgtcgtg
     5821 agtcgttgca gtgcagcgtg tcctgggctg gccgcggcga gcccctctcg agggacgggc
     5881 ttccgctcgt cctggaacgt gctctgcggc cgatttgttg ccatgtcgtt cgtctttttc
     5941 aacctcccgc gctagaagcg aaaaatattc aggaacgcca cttccagcgc gagcgcctcc
     6001 tggatgttgc ggccgagatg atcgcgcaat tcctccaacc gccgcaggcg ccgcagaatt
     6061 tccggagtcg ttaggcggcg cgcactcgcc tccgtgctgg cgcgcgccgt cggtagctcg
     6121 cgggccggga cgccggtgct gctgcggaga acgtcccccc accagcggaa aaggatttcc
     6181 accaactcgg cgcgccggtg caggtaaaga ctttccgtca gcgccttgta atgctcttcg
     6241 cggcgttcga gccaggcgcc gtcggtggtg ttccggtagc gcgtttcttc gcgctgctgc
     6301 gcctcgtcat tttctccctc aatccttcgc cggattccgc cgagcaggcg ctggaaaccc
     6361 tgcaccagcc ggtaggcttg gtgcacatcg cccgacccga ctgcggcgtg gccgttgagt
     6421 aactcgacca attcattttc ctcgtccgaa agtttcgcgt cggtcggagg cgtgagcgga
     6481 acggccaggc aacgtgaccg aatcgtatcg ggcaggacct ccgggatcgc gctgagcagg
     6541 aggagaagcg aattgttagg cggttcctcg agcgtcttta aaaaggcgtt cgccgcctgc
     6601 tcaatcatcc gatcggcctc ggaaatgatg gccaccttcc gtcggccgtc gctggcccgc
     6661 atctggagcg cgtgttccag ggcgcggatt tgttcgatca cgatgcggcg cgatttcgac
     6721 tccgggctgg cgaggtaaac atccggcgcg ctccccgcga agacagcccc cggggaggcg
     6781 ccgttgacca gatgcgccac gtccgccgcg aaggcgcgct tgccgctgcc ggttgcgccc
     6841 gtgatgaggt aggcgtgccc gagacggctg cgtgcctggg cctcgcgcaa ataatcgagc
     6901 gcaactttcg gcgtgaaagc cacggctagg agcgccggcg ttggcggagc aacgggagca
     6961 aaggcgagag atgaccgaag cgcgtcgcaa tgatctccca gatttcctcc tcgagctctt
     7021 ccaccgtccg cgtcgcatcg atcaacctga tccgctgcgg ttcgcgcgcc gccagttcgc
     7081 gatacgccgc gcagaccgcc tcgtaaaatt tcagcggttc ctgttccatc cggtcaatgc
     7141 cggcggccgg ccgcacccgg cgcatcatgc gggtgcgcgc cgtttcggca tcggtgtcga
     7201 ggacgaaggt gagatccggc cagcaattgc cgacggcaaa agcgttcagg tgcccgatca
     7261 tttcatggtc gagtcggcgc gcaacaccct gatagacagt ggtggaatcc gcgaaacgat
     7321 cggagagcac gaccgtgccg cgatcgagcg caggttgaat cacttcccgg acgagctggc
     7381 tgcggctggc ctcgaaaagg agcagctccg tttccggtgt catggccacg ctctcgggtg
     7441 cgaattgcag gagatggcga atcctttcgc cgatcggcgt cccgcccggc tcgcgggtga
     7501 cgaggagcgg cacgccggcg ttcgttaggc gagcggccag taatcccacc tgggtcgatt
     7561 tgccgcagcc ttccgaacct tcgaaagtga taaaagggag gcggggcatg acgccgattg
     7621 tgaagcaact cgcaagggtt gtcgaatctt accttgcccg cggccgggcg caggaatcgc
     7681 caacctcggt gggacgagct tccgctcgtc catggccatc cgaaagcatg gccctccgct
     7741 ggtgtgatgt aacagagtaa ttgaacacac ttcctccgaa tgcgtctccg gcagtctggg
     7801 gagcgcacgc gacccgcgtg gcgctggcga ctcgccagcg cgaacttagc ctaaccttcg
     7861 ccgcagacgc gtttcccctt cgatccaagt tcgtggcggc gagtcgccgc cacccgcacg
     7921 cgagtcgcgt gcgctcccca aaagttgcaa ccgtgctggg cccgacttca aaattacacc
     7981 gcactaatgc tccgagcgct tcagcagctc gaccaactga tccttccaca gcgcggccat
     8041 cgtgtgggtt ccgtggccgc gcgttttgtc gcttagtgga atcacgatcg cgcgcccttt
     8101 cggcacccgc ttgatctcac gctcgaggat cccgagttcc ggcggattga tcaggtcgtc
     8161 ggccgaattg atcgcgagca gcggcgcgcg aattttttcg agattgggac ccggatcata
     8221 atcgcgcgac gcctcgacgg cgtagaggac gtcgttcgcg tcgctcgtct tgacgtcatc
     8281 attcccaaat ttgtccagca cctcgtcggt cctggcgagg gtgggcgctt ccttttgtcg
     8341 caggatcgga ttgctgctca tgaaccaaag catttccgcg gcggttcgca ggctcggtgg
     8401 ctgggtctgg tagtcgccgc catgccaggc gggatcgttg cgaatcgcgt cgatgacgat
     8461 gcggcgccaa gcgcggttgc gtccggaaat ttgtgtcggg agactcgcta gcggcatcaa
     8521 tgcgtccatg aattcgggat gcgtttcgcc ccagagccag gtgtgcatcc cgcccatcga
     8581 cgtgcccatg atgagacgcg tgtgatcgac ccccaggccg tcggtcagca gccggtattc
     8641 cgcctcgacc atgtcgcggt aaccgtagtt cggaaatttc gcgtgcatcc catcgctcgg
     8701 tttgctcgat ttgccgtggc cgatgtcatc cggcaggacg ataaaaaact tcgcggcatc
     8761 gagcggttgg cccggcccga aaagttcgcc ggcgaattcc ggtcggatga attgcgcgcc
     8821 actgccggtg gtgccgtgca tgatgagcac ggcgttttgc gtgacgccgt gcgcgtcctt
     8881 cgccggtttg ccgagcgtgc ggtaatgcag ccggagctcc gggagtgtgg ctccggacgt
     8941 gaatttgaag tcgcgcgcga taaagtcgcc ttcgtgaggc gcgggataat cggccgcctg
     9001 caaagcggcg gtcaaagcca ccgaccagat caacaccgcg attcgaagtc tcatgcggga
     9061 agagtacaga aaagacaccg ccgtggccac gagaatagga cgtcggaccg gggagagatc
     9121 gtcatcctga gcgcagcgca gcggagtcga aggatcccgc ggcgctggct taaaggttac
     9181 gccacgggct ccttcgactg cgctcgggat gaccgcgaac gccgggtctc gaacaaagag
     9241 acaaacgcgt gatttcccag acgcgccgat tgtgttatgg gatcgccatg aagaaatact
     9301 ggctcgtgaa acaggagccg gaagcctatt cgtggaacga ctttgtgcgc gacggcctaa
     9361 cgaaatggac cggcgtgcgg aatttccagg cgcgcaataa tctccgcgcg atgcgcaagg
     9421 gtgatgctgt cctctattat catagcgtga gtgagaaggc cgcggtcggc atcgcgcagg
     9481 tgacgcgcga a
//
LOCUS       DATMNS010000168         3411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_951026, whole genome shotgun sequence.
ACCESSION   DATMNS010000168 DATMNS010000000
VERSION     DATMNS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951026"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..821)
                     /locus_tag="VNW28_05795"
     CDS             complement(135..821)
                     /locus_tag="VNW28_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXA09474.1"
                     /translation="MIAKKVRAFAAYRYLRGSGIEVGALNSPLPIYHGGKVRYLDRLT
                     TEQLRSAYPEIPAQDFVDVEQVEDGEKLASIDAESCDFVIANHVIEHARNPIGALENM
                     LRVLKPDGILYLALPDKRFTFDAERAVTSYEHLKHDYFESPESSDAEHYLDWITAVGK
                     VTDAAEAAGRAAQIRATKGDIHFHVWTQREFIELILRLRADFHWPIEIEAVIKNGQEF
                     VVVLRKADFG"
     gene            complement(939..3380)
                     /locus_tag="VNW28_05800"
     CDS             complement(939..3380)
                     /locus_tag="VNW28_05800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXA09475.1"
                     /translation="MEPGIYSQSKARRGDLAILLALAAARLVLHSATNWQYGFHRDEL
                     GVLDDARYLAWGYVSYPPFTPLIARVALILFGPSLVGVRFFAALAQSIAMVFAGLMTR
                     ELDGRRWAQVVAALATATAPVALAMGTLFQYVSFDFLWWVMAAYFLIRLLREDNPRWW
                     LAIGAVLGLGMMTKYTMIFFIAGLGAGVLFTAARRYLKSPWLWAGAALSVLIFLPNLI
                     WQTQHHFISLTFLNHIHARDVRIGRTDGFLIGQLTDCANLFTLPLSVAGLYFYLVAPN
                     GRRYRALGWMFAVPLVLFVLARGRSYYLAAAYPMLCAAGAAVWDDWLAARSPGTARVG
                     RAVTWVALGAGAIPGVLLTLPLAPINSPLWRLTSKVHDNFTEQIGWPELAQTIAGIYA
                     ALPEKEKPHAAILAGNYGEAGAINLYGRAYHLPGVICGTNTYWWRGYGAEPPAVVILV
                     GFSREDAARLANRVELAGHVTNRFGVLNEETRDHPDIFVCRDFKKSWPEFWKNFQRFG
                     ERGKIMQRRRSRVRVAGEEEGKRQLPNEMLIKIGFDIEFALRGPTPMVLMLFVHPSRE
                     GDLRSPQELRVEPKLPVSYYTDLFGNRCARLLAPAGNLRLALETLIEDSGQPDPQSPD
                     AAQHKIENLPNEALAFLMPSRYCEVDKLSDIAWKLFGDTPPGWPRVEAICTWVNQHIT
                     FGYHFADPSKSAFDVYQSGEGVCRDFSHLALTFCRCLNIPARYVTGYMGDIGVPVVLP
                     MDFSGWFEVYLGDRWHTFDARHNVPRIGRIVMAVGRDAADVALTTNFGSATLKKFFVI
                     SDEVNASNQGAMRIS"
BASE COUNT          689 a         1079 c         1032 g          611 t
ORIGIN      
        1 ccatcccgag ttgctcgtct ggttccccaa cgtcctcttc atcgtcatcg gcgcctacct
       61 ctttcgccgc ctgagcaagc agtaatcccc ggcggccata agtgagtctt gagcgcgcag
      121 agatacgcgg tggcctagcc aaaatccgcc ttgcgcagaa cgaccacgaa ttcctgcccg
      181 ttcttgatca cggcttcgat ttcgatcggc cagtggaaat cggcgcgcag ccgcaaaatc
      241 agctcgataa actcgcgctg cgtccagacg tggaaatgga tgtcgccttt ggtcgcccgg
      301 atttgggcgg ctcgcccggc cgcctcggcg gcatccgtga cttttccgac cgcggttatc
      361 caatcgagat aatgctccgc gtccgacgat tccggcgatt cgaaatagtc gtgcttgaga
      421 tgttcgtagg aggtaacggc ccgctcggcg tcgaacgtga accgcttgtc cggcagcgcc
      481 aggtatagaa ttccatccgg cttcagcaca cgaagcatat tttcgagcgc tccgatcggg
      541 ttgcgagcat gttcgatgac gtgattggca atcacgaagt cgcagctctc ggcgtcgatc
      601 gaagcgagtt tttcgccatc ctcgacttgt tcgacgtcca cgaagtcttg cgcggggatt
      661 tccggatagg cggaacgaag ttgctccgtc gtcagccggt cgaggtagcg aactttgcct
      721 ccatgataaa tcgggagcgg ggaattcagc gcgccaacct cgattccgct accgcggagg
      781 taacggtagg cggcaaacgc gcggactttt ttcgcgatca cgctccaagg cgtggttccg
      841 tccgccggtt cgtcacctgc cggatggcaa cgcggcggtc tgcgaaagtt gcggacgaaa
      901 cgaagcaatc ccacggcgtc ccagcttaaa atctccgcct aactaatgcg catcgcccct
      961 tggttcgacg cgttgacctc gtcgctaatc acgaagaact tcttcaaggt cgcgctgccg
     1021 aagttcgtcg tgagggcgac atccgccgcg tcgcgcccga ccgccatcac gatgcggccg
     1081 atgcgcggca cgttgtggcg ggcgtcgaag gtgtgccagc gatcgccgag ataaacttcg
     1141 aaccagccgc tgaaatccat cggcaagacg accggcacgc cgatgtctcc catgtagccg
     1201 gtcacgtagc gcgccgggat gttcaggcag cggcaaaagg tgagcgcgag atgactgaaa
     1261 tcgcggcaaa cgccctcgcc gctctggtaa acatcgaagg ccgatttcga cggatcggca
     1321 aagtgataac caaaggtgat gtgctggttt acccaggtgc agatcgcctc cacccgcggc
     1381 cagccgggcg gcgtgtcgcc aaaaagcttc caggcgatgt cggagagttt gtccacctcg
     1441 caatagcggc tcggcatgag aaaggccagc gcctcgttag gcaaattctc gatcttgtgc
     1501 tgcgctgcat ccggcgactg cggatcgggt tggccgctgt cttcgatcag tgtctcgagc
     1561 gcgaggcgca ggttgccggc gggcgcgagg agccgcgcgc accggttccc gaaaagatcc
     1621 gtgtaatagg ataccggtag cttcggctcg acccgcagct cctgcgggga gcgcaaatcg
     1681 ccctcccgcg aaggatgcac gaaaagcatc agcaccatcg gcgtcgggcc gcggagcgca
     1741 aactcaatgt cgaatccgat tttgatcagc atttcgttag gcaattgccg cttgccttcc
     1801 tcttcgcccg ccactctcac cctgctgcgc cgccgctgca ttatttttcc gcgctcgccg
     1861 aagcgctgga aatttttcca aaactccggc cacgatttct tgaaatcgcg gcagacaaag
     1921 atgtccgggt gatccctggt ttcttcgttc aacacgccaa agcgattcgt cacgtgcccc
     1981 gccagctcga ctcggttcgc gagccgcgcg gcgtcttcgc gggaaaatcc caccaggatc
     2041 acgaccgcgg gcggctccgc cccgtagccg cgccaccagt aggtgttggt cccgcagatc
     2101 acccccggta gatgataggc gcggccgtag agattgatcg cgcccgcctc cccgtaattg
     2161 ccggcgagga tggcggcgtg cggtttctct ttctccggca gcgccgcgta gatgccggcg
     2221 atcgtttgcg ccagttcggg ccagccgatc tgctcggtaa agttatcgtg caccttgctc
     2281 gttaggcgcc agagcgggga attgatcggc gccaggggca gcgtcaagag gacgccgggg
     2341 atcgcgcccg ctcccagcgc gacccaggtc acggcccgcc cgacccttgc cgtgcccggg
     2401 ctccgcgcgg cgagccaatc atcccacacc gccgcgcccg ccgcgcaaag catcgggtaa
     2461 gccgccgcca ggtagtagga tcgtccccgc gccaaaacga acaagacgag cggcacggcg
     2521 aacatccatc cgagcgcgcg atagcgtcgc ccgttgggtg cgacgaggta gaaataaaga
     2581 cccgccaccg acaagggcag cgtgaagaga ttcgcgcagt ccgtcagttg gccgatcagg
     2641 aaaccatcgg tccggccaat ccggacgtcg cgcgcgtgga tgtgattcag gaaggtgagc
     2701 gaaatgaaat ggtgctgcgt ctgccagatg agattcggca gaaagatcag aaccgaaagc
     2761 gccgcaccgg cccagagcca cggacttttc aaatagcgcc gcgctgctgt gaataggacg
     2821 cccgcgccga gtcccgcgat gaagaagatc atcgtatatt tggtcatcat tccgagaccc
     2881 agaacggcgc cgatcgcgag ccaccagcgc ggattatcct cacgcaacag gcggatcaga
     2941 aaatacgccg ccatgaccca ccaaagaaag tcgaaggaga cgtattggaa cagcgtcccc
     3001 atcgcgagcg ccaccggcgc ggtcgcggtt gcgagcgcgg cgaccacctg cgcccagcgg
     3061 cgtccgtcga gttcccgggt catcaagccg gcgaagacca tcgcgatgct ttgcgcgagc
     3121 gcggcaaaga agcgcacccc gacgagcgac ggtccgaaaa gaatcaatgc gacgcgcgcg
     3181 atgagcggcg tgaaaggcgg ataagaaacg tagccccagg cgagatagcg cgcgtcatcg
     3241 agcacgccga gttcatcgcg atgaaatccg tattgccagt tggtcgcgct gtgcagaacc
     3301 aaacgcgccg ccgccagcgc cagcaggatc gcgagatcgc cccgtcgggc tttcgattgc
     3361 gaataaattc cgggctccat ctgcggcctc tgcaggctat cgattcagcg c
//
LOCUS       DATMNS010000169         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_237882, whole genome shotgun sequence.
ACCESSION   DATMNS010000169 DATMNS010000000
VERSION     DATMNS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237882"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VNW28_05805"
     CDS             <1..200
                     /locus_tag="VNW28_05805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09476.1"
                     /translation="LEAMDGRVLVEALADSKPVPAVRTQELETQSDLGDFTWRQTLRL
                     TTVGKITYLVDGNGGRIPRKP"
     gene            complement(251..1390)
                     /gene="bshA"
                     /locus_tag="VNW28_05810"
     CDS             complement(251..1390)
                     /gene="bshA"
                     /locus_tag="VNW28_05810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HXA09477.1"
                     /translation="MEIARPLNIGITCYPLIGGSGILATALGSELALRGHSVHFFSSA
                     LPIRLDLAQPGIFFHEVTVNEYSLFTYPDYTLPLAVKMAQIARAAELDIFHVHYAVPH
                     ATAAYLAVQMLAGSPARAPRIVTTLHGTDTTLLGQDPSYRPAIEHALSHSDAITTVSE
                     SLRRETIATFQLARPVEVIRNFFVPSVRLRSREEVRRELGLTNDEFLVVHISNVRPLK
                     RIDLLLRSFAAARSSRPLRLLILAGGSFRPYEPLLDQLGLRDRVVVKERVREVEEFLN
                     AADAGLYTSESESFGLSILETLFHGKPVVAFGIGGIPEVVVDGKTGFLHPFGDIAAVA
                     RSLALLADSPELAQEMGAHGRQHAESNFTADRIVPEYESVYRRIF"
     gene            complement(1392..2108)
                     /locus_tag="VNW28_05815"
     CDS             complement(1392..2108)
                     /locus_tag="VNW28_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXA09478.1"
                     /translation="MPETPLRILAIGAHPDDLEFGCGGILLGEMARGSEISLCVCSRG
                     EAGSNGTPDEREAEARRGAKLLGATIEFIELGGDAHLALSVANNIAVARQIRTARPDV
                     LLAPVASRDQHPDHVVVSHLCQNAARLARYGGLAELRDLPPHTIRHHLEYAITPGAEP
                     VRDQPTIRIDISEHLARWMELMECHQTQLRTRRYLELQIARARLLGLAAGVEYAQALY
                     ATDDLLIRSLAELPVSVRLF"
     gene            complement(2101..>2764)
                     /locus_tag="VNW28_05820"
     CDS             complement(2101..>2764)
                     /locus_tag="VNW28_05820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09479.1"
                     /translation="GEIVFFYHDSDHDPRETITILRDRQSGHEQRLNFRFANKLQKQF
                     SPLFAKRVLPEWRKQMARQLPNYVPAQLVGYFANVDAGNVADFCLEMYRQLGLLEGLR
                     VMRSGDPAFRRRAAAVDDYFVDVTYENELVRARISPAGLLLHQGGESYLRLPLEEYDA
                     TQISPTRDTRLRWMQSVIGCTHYVAGASEIKYLNTDEAPGVQFIARDEISESDRAYLP
                     DA"
BASE COUNT          579 a          812 c          878 g          495 t
ORIGIN      
        1 cgttggaggc gatggacggc cgcgtcttgg tggaggcact cgccgactcg aaacccgttc
       61 cagcggtcag gacgcaggaa ctcgaaacgc aaagtgatct gggcgatttc acctggcgcc
      121 agacgctgcg cctaacgacg gtgggaaaga tcacctattt ggtggacgga aatggcggac
      181 gcattcccag gaagccgtaa acaactacca gagaaggcag acgctcagcg cgacttgtcg
      241 ttcccggcgg ctaaaagatc cgccgataaa cggattcgta ttccggtacg atccgatcag
      301 cggtgaagtt ggactcggca tgttgccggc cgtgcgcacc catctcttgc gccagttcgg
      361 gcgagtcggc gagcagcgcg agcgagcggg ccaccgctgc gatgtcgccg aagggatgca
      421 gaaagcccgt cttcccatcg accacgactt ccggaatacc gccaatccca aacgcgacca
      481 ccggttttcc atgaaaaagc gtttcgagaa tactgagacc gaagctctcg ctctccgagg
      541 tatagaggcc ggcatccgcg gcattcagaa attcttccac ttcacggacc cgctccttca
      601 cgacgacgcg atcacgcaaa ccgagctgat cgaggagcgg ctcataagga cgaaacgacc
      661 cgccggccag gatgaggagc cgcaacggcc gcgagcttcg cgcggccgcg aagcttcgga
      721 gcaaaagatc gatccgcttg agcggacgga cgttggaaat atgaacgaca agaaattcgt
      781 cattcgttag gccaagctcc cgccgcactt cctcgcgcga gcgcagacga acagatggaa
      841 caaaaaagtt ccggatcacc tcgaccggcc gcgccagctg aaaagtcgcg atcgtttccc
      901 gccgcagact ttccgagacg gtcgtaatcg catccgaatg cgagagagca tgctcgatcg
      961 ccggtcgata acttggatcc tgtccgagca gagtcgtatc cgtcccgtgc agagtggtca
     1021 cgatccttgg cgcgcgggcg ggagaacccg cgagcatctg gaccgcgaga taggcggcgg
     1081 tggcgtgtgg caccgcgtag tggacgtgga aaatgtcgag ttccgctgcg cgcgcgatct
     1141 gggccatctt caccgccagc ggcagcgtgt aatcaggata agtaaagagg ctgtattcgt
     1201 tgaccgttac ttcgtggaag aaaattcccg gctgcgccag atcgagacgg atgggcagcg
     1261 cggaactgaa gaaatggacg ctgtgaccgc gaagagccaa ctcggatcca agcgcggtcg
     1321 cgaggatgcc gctcccgccg atcagcggat agcaggtgat gccaatgttg agcgggcggg
     1381 caatttccat cctagaaaag gcgcacggaa accgggagtt cagccaggct cctgatcagg
     1441 agatcgtcgg tcgcgtaaag ggcctgggca tattcgacgc cggcggcgag gccgagcagc
     1501 cgcgcccgcg cgatctgcaa ctcgaggtag cgtcgggtgc gcagttgggt ctgatggcat
     1561 tccatcagtt ccatccaacg cgcaagatgt tcgctgatat cgatgcggat ggttggctga
     1621 tcgcggaccg gctcggcgcc cggcgtaatc gcgtattcca aatgatgccg gatcgtgtgt
     1681 ggcgggagat cacgcagttc cgccaaaccg ccgtagcgcg caaggcgcgc ggcgttctgg
     1741 caaagatgac taacgacaac gtggtcggga tgctgatcgc gggaagccac tggcgccaga
     1801 aggacgtcgg gccgcgcggt ccggatttgt cgcgcgactg cgatattgtt cgcaacggag
     1861 agtgcgagat gcgcgtcgcc tcccagttcg atgaattcga tcgttgcgcc gagcagcttc
     1921 gccccgcggc gcgcttccgc ttcgcgttcg tcgggcgtgc cgttgctccc cgcttcaccg
     1981 cgcgaacaga cgcaaagcga gatctcactt ccgcgcgcca tttcgccgag cagaattccg
     2041 ccgcaaccaa actcgaggtc gtccgggtgc gccccgatgg ccaggatacg aagtggagtc
     2101 tcaggcatcg ggaagataag cgcggtccga ttcggagatt tcgtcgcgcg cgatgaattg
     2161 gacgccgggc gcttcgtccg tgttcagata tttgatctca ctcgcgccgg ctacgtagtg
     2221 cgtgcagccg atgaccgatt gcatccagcg caggcgggtg tcgcgcgtcg ggctgatctg
     2281 ggtggcgtcg tattcctcga ggggcaacct cagatacgac tcgccgccct gatggaggag
     2341 aaggccggcg gggctgatcc gcgcgcgcac caattcattt tcgtaagtca catcgacaaa
     2401 ataatcgtcg acggcggcgg cccggcggcg aaacgcggga tcgccggagc gcatcacccg
     2461 cagtccctcg agcagaccga gttgccggta catctcgaga caaaagtcgg cgacgttccc
     2521 ggcgtcgacg ttcgcaaaat acccgaccag ttgcgctggc acgtaattgg ggagttggcg
     2581 ggccatttgc ttccgccatt ccggcaggac ccgcttggca aaaagcggcg agaattgttt
     2641 ttgcagcttg ttcgcaaacc gaaaattcag cctctgttcg tgaccgcttt gccggtcgcg
     2701 caggatggtg atcgtctcgc gcggatcgtg atcgctgtcg tgataaaaga aaacgatctc
     2761 acct
//
LOCUS       DATMNS010000170         4796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4887244, whole genome shotgun sequence.
ACCESSION   DATMNS010000170 DATMNS010000000
VERSION     DATMNS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4887244"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2393)
                     /locus_tag="VNW28_05825"
     CDS             complement(<1..2393)
                     /locus_tag="VNW28_05825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09480.1"
                     /translation="MLFESFFQDLRIGLRMLFKDKTFFFLAVTVLALGICGVTTQFTM
                     VNAVVLRGFSFPHPEQLMSVGLIDPQASDQNNNFGNGNIPSAQDYEDLRAGQKSFALM
                     CAYLNGSTINVTYNKTPQRYTGAYVTEDFLKIIDVKPILGRDFTAADNKPGAEKVTIL
                     GNEIWKRDFNGDPRVVGQAIRINGKAATVIGVMPPNFKFPVNEELWVPLYNEFPPKPR
                     GDPSGTGPAIMGRLKPGVTPDQVNAEFIGLAKRIAKDNPKTNGQLVSAAVLPLLNAFT
                     GPQLRQIIYAMLGAVLVVLLIACVNVMNMQFGRSALRARELAIRGALGATRWRIVRQM
                     LTESFLIAFLGAVVGVAMAYWAVDLLVRTTSTPQFQLPYWIVFSIDAKVLGFTLGAVL
                     LATLVSGLVPALLAARGNASEMMKEGGRGNSSRLVNVITRVLVVGQIAMTAALLIASA
                     LQIRSIRNQVKLDYGYDENGVYTARMALFEGAYPNDDARRQFFVRAVRSLRANPAFDG
                     AAMSDRFRMTFANFGQYEVDGKAYTTDRDRPQGNSEAVSDSYFGTMGLKILEGRDFTL
                     EDSDTKQPVAIVNASFARKYWGNESPLGRRIRRFNPGQPQSWRTIVGVVPDTLMQGPF
                     NREADSAGFYVPLLGASPAPQFCTLIVRPHAGQAAETLGPALSKAVAQLDSDLPVYFP
                     GTPARSHAEFLSGPRLVASLFSIFGVVAFLLSAVGLYGVMSLSVNQRTQEFGIRMALG
                     ADAARIFRMVMTQGAWQLLIGLFLGIGAAALLLGVVAAGALQNILFKVNALDPFIYTT
                     "
     gene            complement(2549..3865)
                     /locus_tag="VNW28_05830"
     CDS             complement(2549..3865)
                     /locus_tag="VNW28_05830"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXA09481.1"
                     /translation="MKTSLKTSRIALTLIVCLTSGLCPAQSMFRGDPAHSGVYPGPGP
                     RTFHRIKWRFPTGDRVVGSPVSSGGVIYFGGDDGNIYAVDAETGHQLWKHVTGGPAPS
                     TPAVVDGTLYAVSYDGKLHALDARTGGVRWKFATGGERRFEAKGIHGMQPRNQTIADP
                     FDVFLSSPVVAQGAVYFGSGDGNLYAVDVASGEQRWKFQTGDVVHASPAYANGVLFFG
                     SWDSYFYAVDAATGQEKWRFHGGEDPAVHNQVGFQSSPAVVDGVVYTGCRDAQLYALD
                     AATGKEKWHFDNALSWVISSPAVSQGKVYFATSDSSLYHVVAAATGKPVLKQQDTAFM
                     FSSPAIAGDVVFIGVMNGTLQARDLQSGEVLWNFETQTSRQNKDWVLAKDRGFDLPLL
                     YHSNWREDPILAADREFRIGAIFSSPLIANGVVYFGSTDGYLYALE"
     gene            complement(3862..4584)
                     /locus_tag="VNW28_05835"
     CDS             complement(3862..4584)
                     /locus_tag="VNW28_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09482.1"
                     /translation="MSKLLRLSAILFFSATLLGEAAEKVAGRWEGSVQIPGGGFNLIV
                     DLDQSGGKDWIGSVIIPGLGVKGAPLTEISVNDAGISFAIKGALASERVGQASCKAHL
                     NERGQLTGDFLQAGNTAPFILQKTGPPQVELPRTSTAVSKDLEGVWKGDFEMTGYPRH
                     VTLTLTNHESGPASAEFVVVGKRTTNAPVDLVLEEGGFLTIESHDIGITYEGRFRKEA
                     GEIKGTFVLGPFELPLVLQRAP"
BASE COUNT          952 a         1439 c         1494 g          911 t
ORIGIN      
        1 gtcgtgtaga tgaaaggatc gagcgcgttc actttgaaga gaatattttg cagcgcgccg
       61 gccgcgacga ctccgaggag cagcgccgcg gccccgatgc cgaggaagag cccgatgagc
      121 aactgccagg caccttgcgt catcaccatc cggaagatcc gcgccgcatc cgccccgagc
      181 gccatgcgaa tcccgaattc ctgcgtgcgc tggttgaccg agagactcat cacgccgtaa
      241 agaccgacgg ccgagagaag aaaagcgacg accccgaaga tgctgaagag agaggccacc
      301 aggcgaggtc cgctcaggaa ttcggcgtgc gaccgtgccg gagtgccggg gaagtagacc
      361 ggcagatcgg agtccagttg ggcgaccgct ttgctcagcg cgggcccgag cgtctccgcc
      421 gcctggccgg cgtgcgggcg cacgatgagg gtgcagaatt gcggcgccgg gctcgccccg
      481 aggagcggaa cataaaatcc ggcgctatcg gcctcccgat tgaacggccc ctgcatcagc
      541 gtgtccggaa ccacgccgac gatcgtgcgc cacgattgcg gttgcccggg attgaatctg
      601 cgaattcgcc gtccgagcgg gctctcatta ccccagtact tgcgcgcgaa gctcgcgttc
      661 acaatcgcga ccggctgttt cgtgtcgcta tcttcgaggg tgaaatcacg gccctcgaga
      721 atcttcaatc ccatcgtgcc gaagtagcta tccgagaccg cttcggagtt gccttgcggc
      781 cggtcgcgat cggtcgtgta agcctttccg tcgacttcgt attggccaaa attggcgaag
      841 gtcatccgga aacggtcgct catcgccgcg ccgtcgaagg ccgggttggc gcggagcgaa
      901 cggacggccc ggacgaagaa ctgccgccgc gcatcgtcgt tcgggtaagc gccctcaaac
      961 agcgccatgc gcgcggtgta aacgccgttt tcgtcgtagc cgtagtccag ttttacctgg
     1021 ttgcggatcg accggatttg caacgcggag gcgatcagga gggcggcggt catcgcgatc
     1081 tggccgacga cgaggacgcg ggtgatcacg ttaacgaggc gactgctgtt accgcgtccg
     1141 ccctccttca tcatctcgga ggcgttgccg cgcgccgcga gcagcgccgg caccaaaccc
     1201 gaaacgaggg tcgcgagcaa gaccgcgccg agcgtgaatc cgaggacctt ggcgtcgatc
     1261 gaaaagacga tccaataagg cagttggaat tgcggagtac tcgtcgtgcg cacgagaagg
     1321 tccaccgccc aataggccat cgcgacgccg accaccgcgc cgaggaacgc gatgaggaaa
     1381 ctttcggtca gcatttgcct aacgatgcgc cagcgggtcg cgccgagcgc gccgcgaatc
     1441 gccaattccc gcgcccgcag agccgagcgg ccgaattgca tgttcatcac attcacgcag
     1501 gcgatgagca ggacgacgag gaccgcaccg agcatcgcgt aaatgatctg gcgcagttgc
     1561 ggaccagtga acgcgttcaa gagcggcaga acggcagcgg aaacgagctg gccgttggtt
     1621 ttcggattgt ccttggcaat gcgcttcgcg agaccgatga actcggcgtt gacctggtca
     1681 ggggtgacgc ccggtttcag ccgacccatg atcgcggggc cggtgccgga aggatcgccg
     1741 cgcggtttgg gcgggaattc gttgtagagc gggacccaca gttcctcgtt caccgggaac
     1801 ttaaagttcg gtggcatgac cccgatgacg gtggcggctt tgccattgat ccggatcgcc
     1861 tggccgacga ctcgcgggtc gccattgaaa tcgcgtttcc aaatttcatt cccgaggatc
     1921 gtcaccttct ccgcgcccgg tttgttatcc gccgcggtga agtcacgccc gaggatcggt
     1981 ttgacgtcga tgatcttgag aaaatcctcg gttacataag cgccggtgta gcgctgcgga
     2041 gttttgttgt aggtgacgtt gatggtcgag ccgttgagat aggcgcacat gagcgcaaac
     2101 gatttttgcc cggcgcgcag gtcttcgtaa tcctgcgccg aggggatatt gccgttgccg
     2161 aagttgttgt tctggtccga agcctgcggg tcgatcaggc cgacgctcat caattgttcc
     2221 ggatggggaa aggaaaagcc gcgcagcacg accgcgttga ccatcgtgaa ttgcgtcgtc
     2281 acgccgcaga tgccgagcgc gagcacagtg acggccagga agaagaacgt tttgtccttg
     2341 aacagcatgc gcagaccgat gcggagatcc tgaaagaatg attcaaaaag catggcggtt
     2401 attcggttga tggtttccgg aaaaaattga agacaacgcg ctgcgccccg tcgcgttgtc
     2461 cggttgagat gccgcgaagg cgtcgtttgg attcagaaaa atcagacgac gccgagtggc
     2521 gttcctgtct cttcccgggc gggtggtact attcgagcgc atagagatag ccatccgtgc
     2581 ttccaaagta gacgacgccg ttcgcgatca gcggcgagga gaaaatggcg ccgatgcgaa
     2641 actctcggtc ggccgcgagg atgggatctt cccgccagtt cgagtggtag aggagcggca
     2701 ggtcaaaccc ccggtccttc gccagcaccc agtccttgtt ttgcctggaa gtctgggtct
     2761 cgaaattcca gagcacttcg ccactctgca aatcgcgggc ctggagggtg ccgttcatga
     2821 cgccgatgaa gacgacgtcc cccgcgattg ccggagacga aaacatgaac gccgtgtcct
     2881 gctgtttcag gacaggtttg ccagtggcgg cggcgacgac gtggtaaaga ctcgagtccg
     2941 acgtcgcaaa gtaaaccttg ccctgcgaga ccgcggggga actgatgacc cagctcagcg
     3001 cattgtcgaa atgccatttc tccttcccgg tcgcggcgtc gagcgcgtag agctgcgcat
     3061 cgcggcaccc ggtgtagacg acgccgtcca cgacggcggg agacgattgg aatccaacct
     3121 gattgtgaac cgccggatct tcgcctccgt ggaagcgcca tttctcttgc ccggtcgcgg
     3181 catccacggc gtaaaaatag ctatcccagc tcccaaagaa gagaacgcca ttcgcgtaag
     3241 ccggtgacgc gtggaccaca tcaccggttt gaaatttcca gcgctgctcg ccggaggcga
     3301 catcgaccgc gtagaggttc ccgtcgccgc tcccaaagta gaccgcgccc tgcgccacca
     3361 ccgggctcga gaggaagaca tcgaatggat cggcgatcgt ctggtttctc ggctgcatgc
     3421 cgtgaatgcc tttcgcctcg aagcgccgct cgcccccggt ggcgaacttc cagcgcacgc
     3481 cccccgtgcg cgcatcgagc gcgtgcagtt ttccgtcgta gctgacggca tacaacgttc
     3541 catcgacgac cgccggcgtg gacggtgccg gaccgccggt cacatgcttc caaagctggt
     3601 gccccgtctc cgcatcaaca gcgtagatat tgccgtcgtc cccgccgaaa taaatcacgc
     3661 caccgctcga gaccggtgaa cccaccacac gatcgcccgt tgggaacctc catttgatcc
     3721 gatgaaaggt ccgcggcccc gggccgggat aaacgccgga atgcgcggga tcgccgcgga
     3781 acatcgactg cgccgggcaa agtccgctcg ttaggcagac gatcagcgtg agggcgatgc
     3841 gtgaagtctt gagggaggtt ttcatggcgc gcgctgcaag acgagcggca gctcgaaggg
     3901 accgagcaca aaagttcctt tgatctcgcc ggcctctttc cgaaaccggc cttcgtaggt
     3961 gatcccgata tcgtgggatt cgatggtcaa aaaaccaccc tcttccagga cgagatcgac
     4021 cggcgcattc gtcgtgcgtt tgccgacgac gacgaactcg gcggaagccg gcccagactc
     4081 gtgattagtc agtgtcagcg tgacatggcg cgggtagccg gtcatctcga agtcgccttt
     4141 ccacacgccc tcgagatcct tgctcaccgc ggtgcttgtg cgcggcaatt cgacttgtgg
     4201 cggaccggtc ttttgcagaa taaacggcgc cgtgttgcca gcctgcagaa agtcgccggt
     4261 cagttggccc ctttcgttca ggtgcgcctt gcaactcgcc tggccgaccc gctcgctggc
     4321 cagcgcgccc ttgatggcga acgagatccc ggcatcattg accgagattt cagtgagcgg
     4381 agcgcccttc actccgaggc ccgggataat gactgatccg atccagtcct ttccgccact
     4441 ctggtcgaga tcaacgatca ggttgaatcc tccgcctgga atctggaccg agccttccca
     4501 tcggccggcg actttttccg ccgcctcgcc aagcaacgtc gcgctgaaga agagaatggc
     4561 agagagtcgc agcaacttgc tcatcggtcg tggttaggcg cggtcggtgg gttaacgtct
     4621 gtgcagatat gcagagggcg taccatccga ggttgaatgt cataactcgt ttgcttgcaa
     4681 cgttttcaga agctcacaac agatccatct caatggctgg cgttccgcct tcgggatttt
     4741 gccgtgtcgg tttcgagatc gggttgatcg tttgccaaag gaaggaattt gtcagc
//
LOCUS       DATMNS010000171         8862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1666752, whole genome shotgun sequence.
ACCESSION   DATMNS010000171 DATMNS010000000
VERSION     DATMNS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8862
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666752"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VNW28_05840"
     CDS             complement(<1..312)
                     /locus_tag="VNW28_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09483.1"
                     /translation="MKTLLTTLTLVSLAAFASAASEENIHETRPAKPGGTLIVNVDFG
                     SIDVAPGENDKVVVDAHRKIQASSKEKEEEYFKAVPVTITTEGDKVIVRAIHQRDSLG
                     KQ"
     gene            complement(415..2919)
                     /locus_tag="VNW28_05845"
     CDS             complement(415..2919)
                     /locus_tag="VNW28_05845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09484.1"
                     /translation="MIIESFTQDIRVGLRVLLKDKVFCLLSILVLALGIGGAMTQFTV
                     VNAIVLRGFSFPHPEQLVSVGLIDPKASDQNNNFGVGNIPAAQDFEDIKAAQHSFAMM
                     AGYLNGSTINVTYKKNPQRYTGAYVTDEMFKILGVLPVLGRDFTAADNKPGAPKTAIL
                     GDEIWRRDFNADPKIVGQNVTINGKAATIIGVMPPGFKFPISEELWTPLYNEWPPTPR
                     GELFLGANSRAPAVMGRLKPGVTLDQANAEMSTIARNIAVDNPKTNQNFTSASVTSLL
                     NAFTGVQLRQIVWAALGAVIVVLLIACVNVMNMQFGRAALRAKELAIRGALGATRWRL
                     VRQMLTESLVVAFFGAIAGTLLAYWGVDLFDKVVQAAPFPPPYWWRFTIDGRVLTFTL
                     AITLLATVASGLLPAFLSSRSNAAEVMKEGGRGNSSRLVNVITRVLVVGQIALTAALL
                     IAATLQIKSIRNQMKLDYGYDENGLYAARLALMEGPYPSEDSRRDFFKSAVRVLRTNS
                     QFEAAAMTDRFRMTFAPAGQYEVDGQKYLTDRDRPRCNFESVSDGYFTTLGLKILEGR
                     DFTIEDNDAKQPVALVNASFARKYWGNQSAIGHQLRIFNPGKEQPWRTIVGVVPDTLM
                     QGPFDQQTEAVGFYMPLLGASPATQFATVVVRPRAGVRADTLGPVLSKAVAALDSNLP
                     TYFAGTPAQFHNDILSGNRVIATLFTIFGGVAFILSGVGLYGVMSFSVNQRRQEFGIR
                     MALGADAIRILRMVMSQGAWQLAIGLVLGAGGVAMLLGVVAAAALKNILFKVNPLDPT
                     IYFAVAGMLTLVAAASCFVPARRASRVDPMVALRCE"
     gene            complement(2916..3614)
                     /locus_tag="VNW28_05850"
     CDS             complement(2916..3614)
                     /locus_tag="VNW28_05850"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA09485.1"
                     /translation="MVEPLIKLRHVERSYPLGKQQFVYILRDIDLEVEEGDFVSVMGP
                     SGAGKSTLLHLLGMHDFGWTGEYYLDETAVHRLKPKERTLLRNEQIGFVFQNYNLLDD
                     LTVYENLDIPLSYRRYGKSERTALVADTLDRFSIVGKKDLYPRQLSGGQQQLVSIARA
                     VIASPKLILADEPTGNLHSSQAEEIMHLFKRLNAEGTTIIQVTHSETNAAHGNRLIQL
                     RDGWIVDNNRDKSS"
     gene            complement(3752..6199)
                     /locus_tag="VNW28_05855"
     CDS             complement(3752..6199)
                     /locus_tag="VNW28_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09486.1"
                     /translation="MISEIRFALRQLIKNPLFALVGILTLALGIGATTAVLTLVNALL
                     IKALPYEDPARLVLLFEHFRDQHLDAIPVSPPEFLEYKAQLKSVEKLAAFTTAAYNLS
                     EGDVPERIFGATVSADLFPLLGVQPIRGRIFRPEECAVGRNDVIMISERLWRRKFDRD
                     PRVLGSKVLADGRVFTVVGIMPASFEFPLPLFNITGAQFGEQADIWQPLGFTDAEMKN
                     RGSRSYGVIGRLAPGASAPQAQAEIETVVRAMRPRFKNNYPQTESFGATLYPLKEQVI
                     GGMKPLLLILLGAVALVLLIACANLATMLLARASAREREMAIRVAVGASRARLLRQGL
                     TESVVLAIFGGAVGVLFAIWAIDLVKTLGTQTIPRLSEVRIDGSVLLVTLAIAIGTGL
                     IFGLVPALATGRPDLTEALKEGGRGSTSSRRHNRLRNALVVAEVALALVLLTGAGLLL
                     KSFVRLENVNPGFNAKNVLTAEISLPALRYPDNKTQAAFFAELERRVATLPGVSQVGL
                     TTILPMSGINSDCSFGIEGRMSDDAHPGPDEEDRIVSPNYFRALEIPLVQGRFFTAAD
                     KLDAPPVVIINRALAERYWPNESALGKRIRVPFTMPAISTIVGIVGDLHHRGLDQPGK
                     PEFYVPVAQVPYSSVILAVRSLSDPAGLTSAIRREVQAIDPAQPIAHVRTLEQVVADS
                     IAPRKLSVVLLAVFAGVALALAAVGIYGVMSFLVVQRTHEIGVRMALGAQRSDVLRLV
                     IFQAGALIAAGTLLGLSVALLSTSLLRSALYGTSALDFATFLLVTLTLALVALLASYI
                     PARRATRADPMIALGRG"
     gene            complement(6196..6402)
                     /locus_tag="VNW28_05860"
     CDS             complement(6196..6402)
                     /locus_tag="VNW28_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09487.1"
                     /translation="MRWNDLNGGSIKTLLILALLAVNVAVAHLRHPEPSNDSGSKDPV
                     ELTLKAPPRDPSPALGLTTIQNAP"
     gene            complement(6424..7122)
                     /locus_tag="VNW28_05865"
     CDS             complement(6424..7122)
                     /locus_tag="VNW28_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA09488.1"
                     /translation="MNNGNHTLIHLDSVTKVFLTDEVETHALSGIHLDIRTGEYVSIA
                     GPSGCGKSTLLSILGLLDTPSGGSYTLKNGEVANLSFPERARIRNREIGFIFQSFNLI
                     GDLTVFENVELPLTYRGMPAGERKAFVNEALERVGMGHRSKHLPSQLSGGQQQRVAVA
                     RALAGKPAILLADEPTGNLDSRNGEAVMDLLKELHAGGATICMVTHDARFAQHADRTI
                     HLFDGRVVEDKIAA"
     gene            complement(7162..8487)
                     /locus_tag="VNW28_05870"
     CDS             complement(7162..8487)
                     /locus_tag="VNW28_05870"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXA09489.1"
                     /translation="MQRSFEVIAGAQPAPTPGFTPVTSSMDVPRPDVARRKKKRRLII
                     AAASVLGLIVITVLVSRLKPAAMSVDRASVWIDTVKRGPMLRQVRGLGTLVPEDIRWI
                     AAAKDGRVEKIVVRPGARVEPDTLIVELSSPDLQQAARDAELQEKGADAELTTLRATL
                     QRELLNQESTTAQVHSEYQQAKMEAETNENLKKNGLIADLAYKTSIIKRDELANRDAI
                     EDKRLAFARDSIEPQLAAKQAAVEQFKASAGMRSQEVEALHVRAGMTGVLQQLPVEVG
                     QRVIPGTNIARVADPTKLKAQIKIAETQAKDIQINQPASIDTRNGIIPGHVIRVDPAV
                     EQGTVTVDVALDGPLPKGARPDLSVDGTIELERLDNVLYVGRPAFAQDGATVGVFKLT
                     PSGEAVRTPVHFGRSSVNTIEILSGLNVGDQVVLSDTSAYDSHDRIRLN"
     gene            complement(8506..8823)
                     /locus_tag="VNW28_05875"
     CDS             complement(8506..8823)
                     /locus_tag="VNW28_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09490.1"
                     /translation="MKTTAQIQDFNQRHASDPRASRRGRSFPQTNQSYQSSTLLGGCG
                     KRVKFDGPSFFEISNRYFADEAPRSFAVEAGVFAALILTAVLPIVNSVQAVATLIRSL
                     GVL"
BASE COUNT         1690 a         2500 c         2939 g         1733 t
ORIGIN      
        1 ctgcttgccc aacgaatcgc gctggtggat ggcgcgcacg atgactttgt cgccttcggt
       61 ggtgatcgtg accggaaccg ccttgaagta ttcttcctcc ttctccttgg aggaggcctg
      121 gattttccgg tgggcatcga ccacgacctt gtcgttctcg ccgggcgcga cgtcgatcga
      181 gccaaagtcg acgttgacga taagcgtgcc gcccggtttg gcggggcggg tttcgtggat
      241 gttttcctcg ctggccgcgg aagcgaaggc cgcgagacta acgagtgtaa gtgttgtcag
      301 cagggttttc ataagggagc gtcagagaag gggttattcg ggtttcatca ttcgtcattc
      361 agctaagtca tttcacgatc taccgatcga tttagagctc gggattggga attttcattc
      421 acagcgcagc gcgaccattg gatcgacacg gctggctcga cgggcgggca cgaaacacga
      481 cgccgccgcg acgagcgtga gcattcccgc gacggcgaag taaatcgtcg ggtcgagcgg
      541 attgaccttg aaaagaatgt ttttcaacgc ggcggcggcg accacgccga gcagcatcgc
      601 gacaccaccg gcgccgagga cgagtccgat cgcgagttgc cacgcaccct ggctcatgac
      661 catgcgcaag attcggatcg catccgcgcc gagcgccatt cgaatgccga actcctgccg
      721 gcgctgattg accgaaaaac tcatcacccc gtagagaccg acgccggaga ggatgaaagc
      781 gacgccgccg aagatcgtga agagcgtcgc gatcacgcga ttgccggaga ggatgtcgtt
      841 gtgaaactgc gcgggcgtgc cggcgaaata cgtcgggagg tttgaatcga gcgctgcgac
      901 ggctttgctc agcacgggcc cgagcgtatc ggcgcgcacc ccggcgcgtg gcctaacgac
      961 gacggtcgca aactgcgtcg cgggcgaagc gccgagcagt ggcatgtaga atccgacggc
     1021 ctcggtctgc tgatcgaacg gaccctgcat caaagtgtcg ggcacgacgc cgacaatggt
     1081 gcgccacggt tgttccttgc cgggattgaa aatgcgcagt tggtggccga tcgcgctttg
     1141 gttgccccaa tacttgcgcg caaagctcgc gttcacgagc gcgaccggct gcttcgcgtc
     1201 attatcttcg atcgtgaaat cgcggccttc gagaatcttg aggccgaggg tggtaaagta
     1261 gccgtccgag acggactcga agttgcagcg cgggcgatcg cgatcggtca gatatttttg
     1321 tccatcgact tcgtactgcc cggcgggcgc gaaagtcatg cgaaagcgat cggtcatggc
     1381 cgcggcttcg aactgcgaat tggtgcgcag cacacgaacg gcgctcttga agaaatcgcg
     1441 gcgagagtct tcgctcggat acgggccttc catcaacgcc aggcgcgcgg cgtagaggcc
     1501 attctcgtcg tagccgtagt cgagcttcat ttgattgcgg atcgatttga tctgcaaggt
     1561 ggccgcaatc aggagcgcgg ccgtcagcgc gatttgtccg accacgagca cgcgcgtgat
     1621 aacattgacg agacgactgc tgttgccgcg cccgccttct ttcatcacct cagccgcatt
     1681 actgcgcgag ctgagaaagg ccgggagcaa accggacgcg acggtcgcga gcagcgtgat
     1741 cgcgagagtg aaggtgagca cgcgcccgtc gatcgtgaag cgccaccagt aaggcggcgg
     1801 gaacggcgca gcctggacga ctttgtcgaa aaggtcgacg ccccaatagg cgagcagcgt
     1861 gccggcaatc gcgccgaaga aggcgacgac cagactctcg gtgagcattt gcctaacgag
     1921 tcgccaacgc gttgcgccga gtgcgccgcg aatggcaagt tctttcgcgc gcagggcggc
     1981 gcgcccgaac tgcatgttca tcacgttcac gcacgcgatc agcaggacga cgatgacggc
     2041 gccgagcgcg gcccagacga tctggcgcag ttgcacgccg gtaaaggcgt tgagcagcga
     2101 cgttacgctg gcggaagtga aattttgatt cgtcttcgga ttgtccaccg cgatattgcg
     2161 cgcgatggtg ctcatctccg cgttcgcctg gtcgagggtg acacccggtt tgagccggcc
     2221 catgaccgcc ggcgcgcggc tgtttgcgcc cagaaagagc tcgccgcggg gcgttggtgg
     2281 ccactcattg taaagcggcg tccaaagttc ctccgaaatc gggaatttga agccgggcgg
     2341 catcaccccg atgatggtcg ccgctttgcc gtttatcgtg acgttctgtc cgacgatctt
     2401 cgggtcggcg ttgaaatcgc gtcgccagat ttcgtcgccg agaattgccg ttttcggtgc
     2461 gcccggcttg ttatcggccg cggtgaaatc gcggccgagc accggtaaca cgccgaggat
     2521 tttgaacatc tcgtccgtca catacgcgcc ggtatagcgc tgcggatttt ttttgtaggt
     2581 gacgttgatg gtcgagccgt tcaggtagcc ggccatcatc gcgaacgagt gctgggccgc
     2641 tttgatgtcc tcgaaatcct gcgccgccgg aatgttgccg accccaaagt tgttgttctg
     2701 atcgctcgcc ttcggatcga tcaaaccgac gctgaccaac tgttctggat gcggaaacga
     2761 aaacccgcgt aacacgatcg cgttgaccac ggtgaactgg gtcatcgcgc cgccgatgcc
     2821 gagcgcaagg acgagaatcg agagaagaca gaagactttg tccttcaaaa ggacccggag
     2881 tccgacgcgg atatcctgag taaacgattc aataatcatg atgatttgtc tcggttgttg
     2941 tcgacgatcc aaccgtcgcg cagttggatg aggcggttgc catgagcagc gttggtttcc
     3001 gaatgcgtca cctgaatgat ggtcgtgcct tcggcgttca gtcttttgaa gagatgcatg
     3061 atttcctcgg cctggctgga atggagattg ccggttggct cgtcggcgag aatgagcttc
     3121 gggctcgcga tgacggcccg cgcgatgctg accagctgct gctggccgcc ggagagctga
     3181 cgcgggtaaa gatccttttt cccgacaatc gaaaagcgat cgagcgtgtc ggcgacgagc
     3241 gcggtgcgct ctgatttgcc ataacggcga taggaaaggg gaatgtcgag gttctcgtag
     3301 accgtcaggt cgtcgagcag gttgtagttt tggaaaacga aaccgatctg ctcgttgcgc
     3361 aggagggtgc gctccttcgg cttcaggcgg tggaccgcgg tttcgtcgag ataatactcc
     3421 ccggtccacc cgaagtcgtg catgcctaac aagtgcagga gagtcgattt cccggcgccg
     3481 gacggcccca tcacggaaac gaaatcgccc tcctcgacct cgagatcgat gtcgcgcagg
     3541 atgtagacga actgctgttt gcctaacggg taggagcgct cgacgtgccg gagtttgatc
     3601 agtggttcaa ccatgtggtg gaggggaatg acgaatcccg agtagcgaat ggtgaaggag
     3661 tgacgaagga caaaggacga aaaggatgcc tgccctttgg cgagcttcgg gtttcggcgt
     3721 tgtttcggat ttcatcattc gacatttctt ctcagcctct tccgagagcg atcatgggat
     3781 cggcgcgggt agcgcgacgg gccgggatat aactggcgag aagcgcgacc agcgcgaggg
     3841 tgagggtgac gaggaggaag gtggcaaagt cgagggcgct ggtgccatag agcgcggagc
     3901 gcaggagcga agtgctgagc agggcgacgc ttaagccaag caaagtgccg gccgcaatca
     3961 gagcgccggc ctggaaaatc accagtcgca ggacgtcgga gcgctgcgca ccgagcgcca
     4021 tccggacgcc gatctcgtgg gtgcgctgga cgacgaggaa cgacatcacg ccgtagatgc
     4081 cgacggccgc aagcgcgagt gcgacccccg cgaaaaccgc caggaggacg actgaaagtt
     4141 tgcgcggcgc gatcgagtcc gcgacgacct gctcgagcgt gcgcacatgg gcgatcggct
     4201 gcgcggggtc gatggcctgc acttcgcgcc ggatcgcgct cgtcaggcct gccggatccg
     4261 acaagctgcg gacggcgaga atgacagagg aataggggac ctgcgcgacc gggacgtaga
     4321 actccggttt ccccggctga tcgagtccgc gatgatgcag gtcgccgacg attccgacga
     4381 tcgtcgaaat ggccggcatg gtgaaaggaa cccggatccg tttgcccagc gcgctttcgt
     4441 taggccaata gcgctcggcg agtgcccggt tgatgatgac aaccggcgga gcgtccaatt
     4501 tgtcggccgc ggtgaagaag cgcccctgca cgagcggaat ttccaaggcc cggaaataat
     4561 tgggtgagac gatgcgatct tcctcatccg gtccgggatg cgcgtcgtcg ctcattcttc
     4621 cttcgatccc gaacgagcaa tcgctgttaa tgccgctcat cggcaagatg gtcgtcaggc
     4681 cgacctggct taccccgggc agagtggcga cgcggcgctc gagctcggcg aagaaggcgg
     4741 cttgcgtttt gttgtccggg tagcgcaacg cgggcaggga aatctccgcg gtcaggacgt
     4801 ttttcgcatt gaagcccggg ttgacatttt ccagcctaac gaaactcttg aggagcaacc
     4861 ccgcgcccgt caggaggacg agggcgaggg caacttccgc gacgacgaga gcgttgcgca
     4921 gccgattgtg ccggcggctt gaagtcgaac cgcggccacc ttccttgagc gcttcggtca
     4981 ggtccggccg gccggtggcg agcgcgggaa cgagaccgaa gatcaggccc gtgccgatcg
     5041 cgatcgcaag agtaacgagc aggacgctgc catcgatccg gacttcgctc aggcgcggga
     5101 tggtctgcgt gccgagcgtc ttgacgagat cgatcgccca gatggcgaac agcacgccga
     5161 ctgcgccgcc gaaaatcgcc aggaccacac tttccgtcag gccctggcgg agcaggcgcg
     5221 cgcggctcgc gccgacggcg acgcggatgg ccatttcccg ctcgcgcgcg gaagcccgcg
     5281 cgagcagcat ggtggcgagg ttggcgcagg caatgaggag aacgagcgcg accgcgccga
     5341 gcaggatgag gaggagcggt ttcattccgc cgatgacctg ttctttgagc ggataaagcg
     5401 tcgcgccaaa gctttcggtc tgcggataat tatttttgaa ccgcggccgc atcgccctga
     5461 cgacggtttc gatttccgcc tgggcctgcg gcgcggaagc gccgggcgcc agccggccga
     5521 tcacgccgta gctgcgcgag ccgcggtttt tcatttcggc gtcggtgaag ccgagcggct
     5581 gccagatatc ggcctgctcg ccgaattgcg cgccggtgat gttgaagagg ggcaagggga
     5641 attcaaagct cgccggcatg atgccgacga ccgtgaaaac gcgaccgtcc gcgagcacct
     5701 tgctcccgag gacgcgcgga tcgcgatcga atttccgccg ccagagccgc tcgctgatca
     5761 tgatgacgtc attgcggccg accgcgcatt cctccgggcg aaaaatgcgg ccgcggatcg
     5821 gttgcacgcc taacaatgga aagagatcgg ccgaaaccgt cgcaccgaaa atgcgttccg
     5881 ggacatcgcc ttccgaaaga ttgtaggcgg cggtggtgaa ggcggcgagt ttctcgacgc
     5941 tcttgagttg ggctttgtac tcaagaaatt ccggcggcga aaccgggatg gcgtcgaggt
     6001 gctgatcccg aaaatgttcg aagagaagga cgagtcgcgc cggatcctca taaggcagcg
     6061 ccttgatgag aagcgcattg acgagggtca agaccgcggt cgtggcgccg atcccgagcg
     6121 cgagggtgag aatgccgacg agcgcgaaga ggggattttt gatcagctgc cggagcgcga
     6181 agcgaatttc tgagatcatg gcgcgttttg aatggtcgtc agcccgagcg caggcgaggg
     6241 atcccggggt ggagccttca aggtcaattc cacaggatcc ttcgaccccg aatcgttgct
     6301 cggctcagga tgacggagat gcgcgacggc aacgttgacg gcaaggaggg cgaggatgag
     6361 gagcgttttg atcgatccgc cgttgagatc gttccaacgc attgttccgt ccttcgttag
     6421 gcgttaggcg gcaattttgt cctcgacgac ccggccgtcg aacaaatgga tggtgcggtc
     6481 ggcgtgctgc gcgaaacgcg catcgtgcgt caccatgcaa atggtggcgc cgccggcgtg
     6541 cagttccttc aagaggtcca tgaccgcttc gccgttgcgc gaatcgaggt tgccggtcgg
     6601 ttcgtcggcg agaaggatgg ccggcttccc ggcaagggcg cgcgccacgg ccacgcgctg
     6661 ttgctggccg ccggagagct ggctcggcaa atgtttcgaa cgatggccca tgccgacccg
     6721 ttcgagcgct tcgttaacga aagctttccg ctcgcctgcc ggcatgccgc ggtaggtcaa
     6781 tggcagttcg acgttttcaa aaacagtcag gtcgccgatg agattaaagc tttggaaaat
     6841 gaagccgatc tcgcggttgc gaatccgcgc gcgctccggg aaggaaaggt tcgcgacctc
     6901 gccgttcttg agcgtgtagc ttccacccga tggcgtgtcg agcagcccga ggatcgagag
     6961 gagcgtcgat tttccgcacc ccgacggccc ggcgatcgag acgtattcgc ccgtgcgaat
     7021 atcaaggtga atgccggaca aggcgtgggt ttcgacttcg tcggtgagga agactttggt
     7081 gacgctgtcg aggtgaatca gggtgtggtt tccgttgttc atgttgtggt ttggttggtt
     7141 aaggtgaaga gaaactcaga gctagttgag gcgaatccgg tcgtgcgaat cgtaggcgct
     7201 ggtgtcggat aagactacct ggtcgcccac attcaggccg ctcaggattt cgatcgtgtt
     7261 gaccgagctg cggccgaaat ggaccggcgt gcggacggct tcgccgctcg gggttaattt
     7321 gaagacgccg accgttgctc catcctgggc aaaagcgggt cggccgacgt agaggacgtt
     7381 gtcgaggcgc tcgagttcga tcgttccgtc cacgctcaaa tcggggcgcg cacctttcgg
     7441 gagggggcca tcgagcgcca cgtcgacggt gacagtcccc tgctcgaccg cagggtcgac
     7501 ccggatgaca tggccaggaa tgatcccgtt gcgcgtgtcg atcgaagccg gctggttgat
     7561 ctgaatatct ttcgcctgcg tctcggcgat tttgatctgc gcttttagtt tcgttgggtc
     7621 ggcgacgcgg gcgatgtttg ttcccgggat aacgcgctgg ccgacttcca ccggcagttg
     7681 ttgcagcacg ccggtcatgc cggcgcgcac gtgcagggcc tcgacttcct gcgagcgcat
     7741 tcctgcggag gctttgaatt gctcgaccgc ggcctgcttg gcggcgagtt gcggctcgat
     7801 cgagtcgcgg gcaaaggcga gacgcttatc ctcgatcgca tcacggttgg ccaattcgtc
     7861 tcgtttgatg atcgacgtct tgtaggcgag atcggcgatg agcccgttct tctttaaatt
     7921 ttcgttcgtc tcggcttcca ttttggcctg ctgatattcc gagtggacct gggcggtggt
     7981 cgattcctgg ttgagcagct cgcgctggag cgtggcgcga agggtcgtca attccgcgtc
     8041 cgcgcctttc tcctggagct cggcatcgcg cgccgcctgt tgaaggtccg gactggagag
     8101 ttccacaatt agagtatcgg gctcgacgcg cgcgcccggc ctaacgacga ttttctcgac
     8161 ccggccgtcc ttggccgcgg cgatccagcg aatgtcttcc ggcacgagag tgccgagacc
     8221 gcgcacctgg cggagcattg ggccgcgctt caccgtatcg atccagaccg aagcgcgatc
     8281 gacgctcatc gccgccggtt tcaagcgcga aaccaacacc gtgatgacga taaggccgag
     8341 caccgaagcg gccgcgataa ttagccgccg cttctttttc ctgcgggcaa catcggggcg
     8401 cgggacatcc atggacgaag tgactggtgt gaagcccgga gtgggcgcgg gctgagcgcc
     8461 ggcaatcact tcgaagcttc tttgcatgat gacctggcgg cgcgcttaaa gcacgccgag
     8521 gctgcggatg agggtcgcga ccgcctggac gctgttcacg atcggcagga ccgcggtcag
     8581 gatcagcgcg gcgaagacgc cggcttccac cgcgaaactg cgcggtgctt cgtcggcgaa
     8641 atagcggttg ctgatttcga agaaggaagg gccgtcgaat ttcacccgct ttccgcagcc
     8701 gccgagcaac gtcgagctct gatagctttg attggtttgc ggaaaactcc ggccgcggcg
     8761 cgaagcgcgc gggtcggatg catgccgctg gttgaaatct tggatttgag ctgtggtttt
     8821 catattcttt ctgctggtta ctggtgagat gcgttcgatc tc
//
LOCUS       DATMNS010000172         3604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_952176, whole genome shotgun sequence.
ACCESSION   DATMNS010000172 DATMNS010000000
VERSION     DATMNS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952176"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VNW28_05880"
     CDS             <1..1533
                     /locus_tag="VNW28_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HXA09491.1"
                     /translation="IEHRREVILRRTRFELRKAEERAETLEGYLIALANLDEFIRIIR
                     GSANRDEARVKLLAFEFTRRQVEQIGVLIRNEGRLTEGRYAFSEHQANQILDLRLYQL
                     TGLEREKIVTEYKELIETIKDLRDILAKEIRVFLIIKKELREIQQKYAGPRLTQLVPD
                     EGEINMEDLIANEGTIISITHGGFIKRTAVSAFRAQRRGGKGVIGMTTREGATQEDEG
                     DFIEHLFTATTHDYLMFFTESGRCYVEKVYEIPEMGRASKGRSIANLLELRPGEKIAA
                     TIRVQSKQSGTGPNVVDETWDENLHIVFATRSGIVKKSNLSDYSNVRKGGIIAIQIED
                     DDCLIDAVLTNGNNEIVLITSEGMSLRFHEEQLRDQGRNTVGVWGIRPEKGDHVVGIA
                     LVNNDAMLLVAGENGVGKRTAFDNYRTQRRGGKGIITMKTGDKTGRVVGALTVTEKDE
                     LMLITTKGQMVRTRISEIRAVGRNTMGVKLMDLRGAEKLQAIAPVVSQEAEETETEEV
                     PT"
     gene            complement(1690..1875)
                     /locus_tag="VNW28_05885"
     CDS             complement(1690..1875)
                     /locus_tag="VNW28_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09492.1"
                     /translation="MKKLILLIPAIALGAMALPSCTTVEKEPAPTTTTTTHTESSVTR
                     TPTSTSTTVTRQSTGGY"
     gene            complement(1950..3005)
                     /locus_tag="VNW28_05890"
     CDS             complement(1950..3005)
                     /locus_tag="VNW28_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXA09493.1"
                     /translation="MKKKVRVGIIGGGWPGQQHARALRASARAVLQALAEPNEKRTAE
                     FRQDYVVRKVYPDYVDLLGDSQVDAVVICLPNFLHFPAGLAALEAGKHVLCEKPPTMN
                     SAEMKVLQEEAEQRGLIYFFGRQFRFAPGMRMARELIAKGELGTIYFAEAIWVRSRGI
                     PLGIGGWFTEKKRSGGGALIDVGIHALDAAWYLMGTPRPVSVTAAVFQNFKHLVQTPV
                     CDVEDAAFAFIRFENDAVVQLKTSWAGNLTDEIPQGDIFGRELSNTTIYGTKATIRLR
                     PLTLFQDEAGKLVDTPLQPRDKADSFVLQMENFIAAIEGRAKPVNDAQQAVYLMEMLD
                     AIYLSSASGREVPIARA"
     gene            complement(3020..3379)
                     /locus_tag="VNW28_05895"
     CDS             complement(3020..3379)
                     /locus_tag="VNW28_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HXA09494.1"
                     /translation="MRRLGIAPVDLEETFARSGGPGGQHVNKVSTAVTLKHRPSGTTV
                     TVQDSRSQARNRVLARERLLDVLERRAEETRQRKRARAAQERRRNSPRPPRLKRKILE
                     SKRQRSLLKKLRAKAID"
BASE COUNT          740 a         1063 c         1099 g          702 t
ORIGIN      
        1 atcgagcatc ggcgcgaggt catcctgcgc cgcactcgtt tcgagttgcg caaggccgag
       61 gagcgggccg agaccctcga aggctatctc atcgcgctgg ccaatctcga tgaattcatt
      121 cgcattattc gcgggtcagc gaaccgcgac gaggcgcgcg tcaaattgct cgccttcgaa
      181 tttacccggc ggcaggtcga acagatcggc gtcctgattc gcaacgaagg gcgcctgacg
      241 gaaggccgct acgctttcag cgaacatcag gccaaccaga ttctggattt gcgcctctat
      301 caactcaccg ggctggagcg cgagaagatc gttacggaat acaaggaact gatcgagacg
      361 atcaaggatc tgcgcgacat tctcgcgaaa gagattcgcg ttttcctgat catcaagaaa
      421 gagctgcgcg agatccagca gaagtatgcc gggccgcgcc tgacccagct cgtgccggac
      481 gaaggcgaga tcaacatgga ggatctcatc gccaacgaag gcaccatcat cagcattacg
      541 catggcggtt tcatcaaacg cacggcggtg agcgcctttc gcgcgcagag gcgtggcggc
      601 aaaggggtca tcggcatgac gacgcgggag ggcgcgacgc aggaggatga gggcgacttt
      661 attgagcatc tctttaccgc gacaacgcat gattacctga tgttttttac cgaatcggga
      721 cgctgctacg tcgagaaagt ttacgagatt cctgaaatgg gccgggcttc caaagggcgc
      781 tcgatcgcga acctcctcga acttcgcccc ggcgagaaga tcgccgcgac catccgcgtc
      841 caatcaaagc agagcggcac cggcccgaac gtggtcgacg agacctggga cgaaaatctg
      901 cacatcgtct tcgcgacccg ctccggcatc gtcaagaaat cgaacctttc cgattacagc
      961 aacgtccgca aaggcggcat catcgcgatt cagatcgagg acgatgattg tttgatcgac
     1021 gccgtcctga cgaatggcaa taacgagatc gtcctcatca cgtcggaagg aatgagcctg
     1081 cgctttcacg aggagcaatt gcgcgaccag ggccgcaata cggttggcgt ttggggcatt
     1141 cgtccagaga aaggcgatca cgtcgtcgga atcgctctgg tgaacaacga tgccatgctc
     1201 ctcgtcgcgg gcgaaaacgg tgtcggcaaa cggaccgcgt tcgacaatta ccgaactcaa
     1261 agacgcggcg gcaaaggaat catcacgatg aagaccggcg acaagaccgg ccgcgtcgtc
     1321 ggcgcgctca ccgtgaccga gaaagatgag ctcatgctca tcacgaccaa agggcagatg
     1381 gtgcggacgc ggatttcaga gatccgcgcg gtcggccgaa acacgatggg cgtcaagctc
     1441 atggacctgc gcggcgcgga gaagctccag gcgatcgccc cggtcgttag tcaggaagcc
     1501 gaagagacgg aaactgagga agtgccaacc taacgacgcg cttgtcggag ggacgtgctt
     1561 ccgcgcgtcc agcttggttg ccttggtccg cgctgaagaa tctggacgtg cggaagcacg
     1621 tccctccgag tgaaagaaaa agggagacgc gagacgtctc cctttctcag aatgaattga
     1681 tctcccggcc tagtagccgc ccgtgctctg gcgggtcacc gtcgtgctcg tgctcgtcgg
     1741 cgtccgggtc acggatgact ccgtgtgggt cgtggtggtg gtggtgggag ccggttcctt
     1801 ctcgaccgtc gtgcaggaag gaagggccat tgcgccgagc gcgattgcag ggattaacag
     1861 aataagtttt ttcataatag gtttgttact aacaaactgt gattcgacag aagttgcgtc
     1921 cccggatggc ttaaaagcga gaattcttct caggcccgcg caatcggcac ttcgcgcccg
     1981 gaggcgcttg aaaggtaaat ggcatcgagc atctccatca ggtagaccgc ttgctgcgcg
     2041 tcgttcaccg gcttcgctct cccctcgatc gcggcgataa aattctccat ttggaggaca
     2101 aaagaatccg ccttgtccct cggctggagc ggcgtgtcga ccagcttccc ggcctcgtcc
     2161 tggaataaag tcagcggccg cagccgaatc gtcgccttcg tgccgtagat ggtcgtgttg
     2221 ctcagctcgc gcccgaaaat gtctccctgc ggaatttcat ccgttaggtt tccggcccag
     2281 gaagtcttga gctggaccac cgcatcattt tcaaagcgaa tgaaagcgaa ggccgcgtct
     2341 tcgacatcgc agaccggcgt ctggacaagg tgtttgaagt tctggaagac cgccgcggtt
     2401 accgagacag ggcggggagt tcccatcaaa taccaggccg cgtcgagcgc atgaatccca
     2461 acgtcgatca gcgccccgcc gccggatcgt ttcttttccg tgaaccagcc gccgatcccg
     2521 agcgggatgc cgcgcgaacg gacccagatc gcttcggcaa agtagatcgt gccgagttcg
     2581 cccttcgcaa tcagctctcg cgccatccgc attcccggcg caaaacggaa ttgccggccg
     2641 aaaaaataaa tcagcccgcg ctgctcggct tcctcctgga gcaccttcat ctcggcgctg
     2701 ttcatggtcg gcggtttttc gcagagaacg tgcttgcccg cttccagcgc ggcgaggccg
     2761 gcgggaaagt ggagaaagtt gggaaggcaa atcaccacgg cgtcgacctg cgaatcgcct
     2821 aacaaatcaa cgtaatccgg gtagaccttg cgaaccacgt agtcttgcct gaactcggcg
     2881 gttcgcttct cgttaggctc agccagcgcc tggagcaccg cgcgcgccga cgcccggagc
     2941 gcgcgggcat gctgctgccc tggccagccg ccgccgatga ttcccacgcg cacctttttc
     3001 ttcacgggcg gagctgcgct cagtcgattg ccttggcgcg aagtttcttg aggagcgagc
     3061 gctggcgttt gctctcgaga atcttccgct tcaaacgcgg cggacgaggc gagttccggc
     3121 ggcgttcctg ggctgccctg gccctttttc gctgccgggt ttcttccgcg cggcgctcca
     3181 ggacgtcgag cagacgctcc cgcgcgagca cgcggttgcg cgcctgtgaa cgcgagtcct
     3241 gcaccgtcac ggttgtcccg ctcggacgat gcttgagagt caccgcggtg gagaccttgt
     3301 tgacgtgctg gccgcccggc ccgcccgatc gggcgaaggt ctcttcaaga tcgactgggg
     3361 cgatacccag tcttcgcatt cgttcctcga gattcattcg tttggccgaa ggatgatcca
     3421 gggccacggc gggttcactg aggtcatcct caactggatt ccgacgagtt tccccccgaa
     3481 tgcggctctc ggtcatgggt ggtgtcaatt gttgtgtgta aaagtttgga aagccgaggc
     3541 ggaacgagcc gtgcattgcc tggaggctct cttcagggca aggtgctgct cttcggcgga
     3601 gaca
//
LOCUS       DATMNS010000173        14217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4056365, whole genome shotgun sequence.
ACCESSION   DATMNS010000173 DATMNS010000000
VERSION     DATMNS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 14217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14217
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4056365"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VNW28_05900"
     CDS             <1..317
                     /locus_tag="VNW28_05900"
                     /inference="COORDINATES: protein motif:HMM:NF025274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease C-terminal domain-containing
                     protein"
                     /protein_id="HXA09495.1"
                     /translation="ADVTGEMTSIGTLFAFILVCAGVWIMRVRNPELERGFRVPAAPV
                     VSILGIIVCGAMIYGLGWTNWMRLIVWLLIGLVFYFSYGKHHSRLNAPPPELPEGIKL
                     SR"
     gene            complement(569..811)
                     /locus_tag="VNW28_05905"
     CDS             complement(569..811)
                     /locus_tag="VNW28_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09496.1"
                     /translation="MAIQSIREFNRAVPFVPYEIRMASGQRYEVPHPDFIFISPRGSF
                     VMIVDAKERPHHLSSLLIESASLRNGARRRRSPKRS"
     gene            complement(927..1688)
                     /locus_tag="VNW28_05910"
     CDS             complement(927..1688)
                     /locus_tag="VNW28_05910"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HXA09497.1"
                     /translation="MKEIETSAANAAAPSAGGFQVSDQPIGRADWAAAARADGLPASY
                     GKDLLYVIARDPKSLFLYWDLNWTPLFAQAGLSPREVQLRVYREDGSIERTQEINPFR
                     GHCYVDVAAAGTGYYCELGCLEGDVWTSLVRSGTTATPAASMSEDVSEDFATLPIHLS
                     FQRLIDIFQATKTERATLANSMAELQENAQALEEAVNGGDGTDPELAALLESARRGDA
                     PTAGQLAQWKQLGARFGGASWGGASGGGFGGSSLA"
     gene            1844..3067
                     /locus_tag="VNW28_05915"
     CDS             1844..3067
                     /locus_tag="VNW28_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00300 family protein"
                     /protein_id="HXA09498.1"
                     /translation="MASETIELRGHIIDSLILPKVLDQIIARGGTFKIGEIKIGENRT
                     DQSFARIEVTAPSADALDELTLRLRQHGAEVMEKRSIQLAATPADGVYPQGFYVTTNQ
                     QTFVRLPDGEIEVEPALMDSAIVVDRAARKATATKFYSIKKGMEVVVGHHGIRVVPQQ
                     RSTARADVFEFMASNVSIEKPKTAVIREMAREFKRVRDEKGKILVVAGPAIVDTGAAE
                     HFEKLIKWGFVNLLFAGNALAAHDIENALFGTSLGVYLEHGSLANEGTQNHMRAINAV
                     RGAGGIRQAVASGLLKSGIMYTCVQNGVDFVLAGSIRDDGPLPDVITDAVEAQKAMRA
                     KLGGVTIAMMMGTMLHSIAVGSLLPATVKTLCVDINPGVVAKLTERGSFQAVGLVTDI
                     EPFLRELTDCMEANR"
     gene            3081..3887
                     /locus_tag="VNW28_05920"
     CDS             3081..3887
                     /locus_tag="VNW28_05920"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HXA09499.1"
                     /translation="MRKLLLCPPDYYGIEYEINPWMSRARGADTPLVQAQWRGLHEKL
                     LSLGCEVHLVPPKPKLPDMVFTANAGLTVGKKFIPSNFRHKERAAEAPLFADWMEQHG
                     YEIVWLPEDQFFEGEGDALFAGDTLFCGYKFRTDVNAHRAVADVLQCLVISVELVDPR
                     FYHIDTCFCPLPDGGAVWFPAAFDEYGQRTIRDHVPNLIDVVEEEAAHFSCNAVVLDQ
                     EIVLPEGTPILMKSLGERGYRCHPLPMSEFLKAGGACKCLTMFLPQREKI"
     gene            complement(3938..5689)
                     /gene="polX"
                     /locus_tag="VNW28_05925"
     CDS             complement(3938..5689)
                     /gene="polX"
                     /locus_tag="VNW28_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HXA09500.1"
                     /translation="MKKEQIVEVLETIATLLELQEENPFKIRAYSNAARSIETWGGNL
                     RALAEENRLEEIPGVGKAIAGKITELALTGASKFYDELRAKFPPGILELFSLPGLGAK
                     KIKALHEKLQVGSIADLEKACLAGRVAELAGFGKTTQEKIALTIANRARHAGSFQLGE
                     VAAEAEQLQEDLRAHPDAGRVSLAGSYRRRREIVRDLDFIVATKAPEAITEFFVRHPL
                     VESVVARGATKTSVRLRSGIQADLRVVSGAEYPFALGYFTGSKEHNIVLRNRALQRGW
                     TLNEYRLAPAATETKAKKPALEIPAVRDETELYRALELDFVPPELRENCGEIEAAAAG
                     RLPRLLEPENLRGTFHCHTSASDGRNSLEEMAAAAQELGLQYLGIADHSRSSVQAGGL
                     DRTRLRAQVATIRGLNKKFDGFRLFAGVECDILRDGSLDYDDDTLGELDYVVASVHSA
                     FQLPEAEMTRRIIRALRNPHVTFLGHPTGRLLLKREGYAVDIPAIIEAAAETGTWIEI
                     NAAPKRLDLDWRWWPLAKEKGVKCVINPDAHGVEKLPELWFGVGVARKGWLTKSDVMN
                     CLPLGKIECELARKRSL"
     gene            5773..6321
                     /locus_tag="VNW28_05930"
     CDS             5773..6321
                     /locus_tag="VNW28_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09501.1"
                     /translation="MATALQILMFTSFRFALFFGFLLFGIIPRGLANPFPRNGPVVPG
                     RVARLRRGIAYAPAKAPLPVKRAIWAANELRSKPYRWGGGHASFFDIGYDCSGTVSYA
                     LAAAGLLRAPLCSNELTRFGDRGQGKWITVYARNGHAFAVIAGLRLDTTAWNGWGDRD
                     APRWQLTLRPPRGFEPRHPDGL"
     gene            complement(6460..6621)
                     /locus_tag="VNW28_05935"
     CDS             complement(6460..6621)
                     /locus_tag="VNW28_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09502.1"
                     /translation="MATDKKKKEVKVQDLKPAKDAKGGVHIAGRQVAGHTVAGRQVAG
                     RTIAGRTLQ"
     gene            complement(6732..6893)
                     /locus_tag="VNW28_05940"
     CDS             complement(6732..6893)
                     /locus_tag="VNW28_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09503.1"
                     /translation="MATDKKKKTVKVQDLKPAKDAKGGVGIAGRQVAGHTVAGRQVAG
                     RTIAGRTLQ"
     gene            complement(7009..7152)
                     /locus_tag="VNW28_05945"
     CDS             complement(7009..7152)
                     /locus_tag="VNW28_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09504.1"
                     /translation="MATNKKKKAVKMQDMKPAKDAKGGVRAPVRQRGPVADHRYVAGR
                     TVQ"
     gene            complement(7558..8556)
                     /locus_tag="VNW28_05950"
     CDS             complement(7558..8556)
                     /locus_tag="VNW28_05950"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXA09505.1"
                     /translation="MPRPLSRAKVEDLTTANEAMKRCLRLAALAAKSAVPVVLLGETG
                     TGKTLLAHAIHNSSARANKPFIAFNASAMSDTLLESQLFGHERGAFTGAQQTVKGKFE
                     LSNDGTLLLDEISEMSPLAQVKILHVLEYGEFERLGSEQMLTCNTRIICATNCSLRER
                     VREGKFREDLYHRLNGLTLLIPPLRERIEELPALIAAELKASAFNEGKTITAIHPEAM
                     DKLLAYPWPGNLRELSHTVRTMTLFCEGRVILPEHVIFPPDLKSSSPPPTPVAEAAPW
                     PNGQADGVRDLSLAGAVARHVRFVYDQAGRNQRRTARLLGISRATLARHLRGKVQK"
     gene            complement(8563..9747)
                     /locus_tag="VNW28_05955"
     CDS             complement(8563..9747)
                     /locus_tag="VNW28_05955"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HXA09506.1"
                     /translation="MPNQLMQRGSGETESEMLPQDPPPWFVRSTAWLFIAMFGVALLA
                     SILIKLPETVTCPFILVPTGGADPIQASRMAVVNRVCVTEGQTVAAGDPLFVLRSDEI
                     RDWGTQLGTLSADLRTHEEGLARADAANAAQVEIKKAEISQAESEVGFREKHAASSRE
                     LLNRMEKLTKNGGISLVEVIQYRLQAAESDKDLSVAQRTLQQVKLERQQLESEHARVH
                     DENMAEIAKLKVRLGALKGDLENSQQNLLSVRAPYAAVVISVANQNAGSVVQMGQELC
                     QLAAIAAKPRGRLTLSETAVPRLAVGQRVRFFFDAFPYQRYGVVNAKLDWVSPSAVTS
                     LAGPHFMALASLDEKELAARRHPLPLRVGMGGEARIVVGQRTIMEYAFEPIRQLRENL
                     RD"
     gene            complement(9761..12880)
                     /locus_tag="VNW28_05960"
     CDS             complement(9761..12880)
                     /locus_tag="VNW28_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HXA09507.1"
                     /translation="MKADDENLLRRSSLFQFLPEEHFEKLRPLLQEENYEFGDLLVRQ
                     GDPADSFYVLLKGRARVVKADQSGNEIALATLKPGDSFGEAALSEGGTRNATVRCSTA
                     VEALRLDRADFLQLAEETPELKHYIEMTARHRALHGFLYEFSNFGRLPVPALRSLVEK
                     LTPVEVAKGDAIIRQGDAPGAMFILEKGRARAFHSDNGTEHNLAFYRDGDFFGELSIL
                     NGSPRAASVEAFSDCRLLALEPDAVRDLKRRYPEFGKLMEERLAQYQVKTEARVPLDF
                     TTEMLPAEVAAHDKIGLDGDKAAPAPAAVEGEDPFADESGLFRKKKGRIKRLEHIEQI
                     DEMDCGAASLGMICRHFGRKVSLSRIRQLCHTSTDGTSLKALCRAATELGLAARALKV
                     SVRNLPFMPLPAIIHWEGNHWMVLYDVTDTYVRVGDPASGLRKIPRKEFEQNWSGYAA
                     LFDYTVAFESAPESKPGFAWLLPFLAKFKTLFLQVMLLAIAITFLELLFPIFTEMVVD
                     KVIVEKNISLLKTILLGMGAAVFFVQASSLAQEYLLSFAAVRLDTAILDYLSRQMLSL
                     PMTYFTSRRTGDIQRRLDGARQVRQFAVQHGIGALLALVQLVGAISLMALYSPSLMFA
                     FLATTPLYGGLMYFSQKVLRPLFAGVEESQGKYSSHQIDAIKGIEAVKAASAENVFRD
                     MMLNEFLSVSRKIFRSSFIVMTYDSVLQTIGMISTAIFLWVGANQVMSGALSIGGFVA
                     FSSLTAMAYSGILRTLGVWDNMQFATVLLNRLNDIFEQEPEQGRDRSQLIPVHSLEGR
                     IEMRGVSFKYGGPEAPNILTDITLDIAPGRMVAFVGRSGCGKTTLIKLIAGLLEPTEG
                     TILFDQTDLKTLNYRDVRRHIGMVLQENHIFNESIARNIAFGDAEPDLDRVLAAAQAA
                     AAHDFVMRLPLGYETKIGESGLALSGGQKQRIAIARAIYNNPPVLIFDEATSALDTES
                     ERAIQDNLGRLTAGRTTIVIAHRLSTIREADSIVVLEKGMVAEVGNHDQLMAQRGLYF
                     YLSSQQLGI"
     gene            complement(12885..13964)
                     /locus_tag="VNW28_05965"
     CDS             complement(12885..13964)
                     /locus_tag="VNW28_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09508.1"
                     /translation="MPETQTTPIEKKIIFTCGDRDFSVRDVIDAAHFRGELEAEWTNL
                     LRCVAAEKKAGEQDLEFNDEVIDTAAEQFRYQHDLITAEETEHWLAERGLALSDFSAY
                     FVRHYWGEQWDEIEPEAIDLFSAPSDLRELLTRELILSGELDRMAERLSRRVAARCAA
                     QGQAIDPQLIAAEEALFFERRGLSEDQLAAWLEQLERDRDWWCEALAMEAIQRRDCAA
                     LLSRQAREHEIAALRLPLTRFEVETIELDSLDAAREALLCAREDGMSMEEVAGEGRYP
                     YRHTEVLLEEIPDDLQQKFLSVHPGDILEPIAHGDGFHLCRIIGKAEPDVDDPIVQER
                     ADNRILDRHFADLTTRYILWQILLA"
BASE COUNT         2600 a         4306 c         4368 g         2943 t
ORIGIN      
        1 cggcggacgt caccggtgaa atgaccagca ttggaacgtt gttcgctttc attctcgtct
       61 gcgccggcgt gtggatcatg cgcgtgcgta acccagaact ggagcgtgga ttccgggttc
      121 cggctgctcc cgttgtgtcc atactgggca tcattgtatg cggcgcgatg atctacggac
      181 tcggctggac gaactggatg cggctgatcg tctggcttct gattggactc gtcttctact
      241 ttagctatgg caagcaccac agccgcctca acgcaccgcc accggagttg ccggagggaa
      301 tcaaactgag ccggtagatc aatcgccaag gatcaaggtc gaagtcgcac tgtttgcacg
      361 ctcctcgccg gcctggcggc gcttgctcgt ctgattcgcc atcgggatgg ggttttactt
      421 ctcctacggc gtgcgtcgga gcgggctggc gcagccgggc acggccggca acaaatctgc
      481 caccggcgag agcgccgaaa gttttccgca ctgcgcggca aacgacaccg tgaagagcgc
      541 ctatttaggc ggcggatcgc actcgtccct acgaacgttt cggacttcgg cggcgtcgag
      601 caccgttgcg cagagaggcg ctttcgatca gtaagctgct caagtgatgt ggccgttctt
      661 tagcgtcaac aatcatgaca aacgaacccc ttggagaaat gaagataaag tccggatgag
      721 gaacctcgta gcgctgcccg ctggccatcc gaatctcgta aggcacaaac ggcactgcgc
      781 gattgaactc gcggatcgat tgaatggcca cgccccaatc ctactgtctc aacccttaac
      841 cctcaaccct cggctgctcg gagtgcgtta gaaggcgaac gaaaaaatga aagagcgccg
      901 atcgcgacgg tggtggagcc cgctcgttag gcaagactgc tgccgccgaa accgcctccg
      961 cttgcgccac cccagctcgc gccgccaaaa cgcgcgccga gttgtttcca ttgcgcgagc
     1021 tgcccggccg tcggcgcgtc gcctcgccgg gcgctttcga gcaacgccgc aagctcgggg
     1081 tcggttccgt ctccgccgtt gactgcctct tccaaggcct gcgcattctc ttgcaactcc
     1141 gccatcgagt tagcgagcgt ggcacgttcg gttttggttg cctgaaaaat atcgatcaag
     1201 cgctgaaagc tgagatgtat cggcagcgtg gcaaaatcct ccgacacgtc ctcggacatg
     1261 ctggccgcgg gcgtcgcggt cgtcccggag cggacgaggc tggtccagac gtcaccctcc
     1321 agacaaccaa gctcgcagta gtaaccggtt cccgccgccg cgacgtcgac atagcaatgg
     1381 ccgcgaaacg gattgatctc ttgcgttctt tcgatcgatc cgtcctcccg gtaaacgcgc
     1441 agctgcacct ctcgcgggga gaggccggcc tgagcgaaaa gcggcgtcca attgaggtcc
     1501 caataaagaa aaagtgactt tggatcgcgg gcgatgacgt aaagcaaatc cttgccgtag
     1561 gaagcaggca aaccatccgc gcgggctgcg gcggcccaat ctgcgcgccc gatgggctga
     1621 tcggaaacct gaaaaccgcc ggcggatgga gcggcggcgt tggcggcgga tgtctcgatt
     1681 tctttcacgt ggtgcgcggc aggagcggtt gcattaatag ctgactgagt gatatggaca
     1741 agcggaaact gggcggtgga aatcctggcc gccaagcagg ttggcgccgg ccccgctccg
     1801 cgcttcaact ggcgcgggag cgttccttgg ctaagttcgc tcgatggctt ccgagacgat
     1861 tgagctgcgc ggacacatca tcgattcact cattctgccg aaggtgctcg atcagatcat
     1921 tgcccgtggc ggcactttca aaatcggcga aatcaagatc ggcgagaacc ggacggacca
     1981 gagttttgcg cggatcgaag tgacggcgcc atcggccgac gcattggacg aactcaccct
     2041 gcgactgcgt cagcacggcg ccgaggtgat ggaaaaaagg agtattcaac tggccgcgac
     2101 gccggccgat ggcgtttacc cgcagggttt ttacgtgacg accaatcagc aaactttcgt
     2161 ccgcctcccg gatggtgaga tcgaggtgga accggcgttg atggatagcg cgatcgtggt
     2221 cgatcgcgcc gcgcggaagg ccacggcgac caaattctac agcataaaaa aagggatgga
     2281 ggtcgtcgtt ggtcaccatg gaattcgcgt cgtgccgcag caacgctcga cggcgcgcgc
     2341 cgatgttttc gagttcatgg cgagcaacgt ctcgattgag aaaccgaaga ccgcggtcat
     2401 ccgcgagatg gcgcgggaat ttaagcgcgt gcgggatgaa aagggcaaaa tccttgtcgt
     2461 cgccggcccg gccatcgtgg acaccggcgc ggccgagcat ttcgagaaat tgatcaaatg
     2521 gggcttcgtc aatctccttt tcgccggcaa cgccctcgcc gcgcacgata tcgagaacgc
     2581 cctttttggg acgtcgttag gcgtttatct cgagcacggc tcgctcgcca atgagggcac
     2641 gcagaatcac atgcgcgcga tcaacgcggt ccgcggggcg ggcggcatcc ggcaggcggt
     2701 tgcgtccggc ctgctcaaga gcggcatcat gtatacctgc gtgcagaacg gggttgattt
     2761 cgtgctcgcc ggttcgatcc gcgatgacgg cccgctgccc gacgtgatca ccgacgcggt
     2821 cgaggcgcaa aaggcgatgc gggccaagct tggcggggtg acgatcgcga tgatgatggg
     2881 cacgatgctg cactccatcg ccgtgggaag cctgcttccc gccaccgtaa aaacgctttg
     2941 tgtggatatt aatccggggg tggtggcgaa gctcaccgag cgcggatctt tccaggcggt
     3001 tggcctggtc accgacatcg aaccttttct gcgcgagctg accgattgca tggaagccaa
     3061 tcgttaggcg gaggccgggt atgcgaaaac tcctcctctg cccgccggat tactacggga
     3121 tcgaatacga gatcaatccc tggatgagtc gcgcccgggg cgccgatacg ccgctcgtcc
     3181 aggcgcaatg gcgcgggctg catgagaagc tgctcagcct cggctgtgag gttcacctcg
     3241 ttccgcccaa gccgaaactg ccggacatgg ttttcacggc caatgccggg ctgacggtcg
     3301 gaaagaaatt tatcccgagc aattttcggc acaaagagcg ggcggcagag gccccgctct
     3361 ttgccgattg gatggagcag cacggttacg aaatcgtctg gttgccggag gaccagttct
     3421 tcgagggtga aggcgatgcg ctctttgcgg gcgacaccct tttttgcggt tacaaattcc
     3481 gcaccgacgt caacgcgcat cgcgcggtcg ccgacgtcct gcagtgcctc gtcatctcgg
     3541 tcgagctggt cgatccgcgc ttctatcata tcgacacctg cttttgcccg ctcccggatg
     3601 ggggcgcagt ttggtttccc gccgccttcg acgagtacgg tcagcgcact atccgcgacc
     3661 acgtgcctaa cctgatcgat gtggtcgagg aggaagccgc gcatttttcc tgcaacgcgg
     3721 tcgtgctcga ccaggagatc gtcctgccgg aaggcacgcc gattttgatg aagtcattag
     3781 gcgagcgcgg ctaccgttgt cacccgctgc cgatgagcga gttcctcaag gcgggcggag
     3841 cgtgcaagtg cctgacgatg tttttgccgc aacgggaaaa gatttaagct ttaaggaaga
     3901 agctttaagt tgggacacgg cctttaagac ttgatcctta aagcgacctc ttccttgcca
     3961 gctcgcactc gattttgcct aacggcaggc aattcattac gtcgcttttc gttagccaac
     4021 cttttcgcgc tacgccaacg ccgaaccata attccggcag cttttccacg ccgtgggcgt
     4081 cgggattgat cacgcacttc actccctttt ccttcgccag aggccaccag cgccaatcga
     4141 ggtcgagccg cttcggggcg gcgttaatct caatccacgt gccggtttcc gccgcggcct
     4201 cgataatcgc gggaatgtcg accgcgtagc cctctcgttt caagagcagc cggccggtgg
     4261 ggtgcccgag gaaagtgacg tgcggattgc gcagcgcgcg gatgatgcgc ctggtcattt
     4321 ccgcttccgg caattgaaag gcggaatgga ccgaggcgac gacgtagtcg agttcgccta
     4381 acgtgtcgtc gtcatagtcg agcgagccgt cgcgcaggat gtcgcactcg acgcccgcga
     4441 agagtcggaa accgtcgaat ttcttattga gcccgcggat cgttgcgacc tgggcgcgga
     4501 gccgggtgcg atcgaggccg ccggcctgca ccgagctgcg gctgtggtct gcaatcccga
     4561 gatattgcag cccgagttcc tgcgccgccg ccgccatttc ttcgagcgaa ttgcgtccgt
     4621 cgcttgcgct cgtgtggcaa tgaaaggtgc cacgcagatt ttccggttcg agcaatctcg
     4681 gcaaccggcc ggccgcggcg gcttcgatct cgccgcaatt ttcgcgcaac tcgggtggaa
     4741 cgaaatcgag ctcgagcgcg cgataaagct ccgtctcgtc ccgcaccgct ggaatttcta
     4801 aggccggctt tttcgccttc gtctccgtcg cggcgggtgc cagccgatat tcgttgagtg
     4861 tccagccgcg ttgcagagcg cggttccgca ggacgatgtt gtgttccttg cttccggtga
     4921 aatagccgag cgcaaacgga tattccgcgc cgctgaccac gcgcaggtcg gcctggatgc
     4981 cggagcgcag acggacactg gtcttggtcg cgccgcgcgc gacaacgctt tccacgagcg
     5041 ggtgcctgac gaaaaattcc gtgatcgctt cgggcgcttt ggtcgcgacg atgaaatcga
     5101 ggtcgcgcac gatttccctt cgccggcgat aactcccggc aaggctgacg cgcccggcgt
     5161 ccgggtgcgc gcgcagatcc tcctgcaact gttccgcctc ggccgcgacc tcgccgagct
     5221 ggaaactgcc cgcgtgccgg gcgcgattcg caatcgttag ggcgattttt tcctgcgtcg
     5281 tcttgccaaa gccggccagc tccgcgaccc gcccggccag gcaagccttt tccagatccg
     5341 cgatcgagcc gacctgtaat ttttcgtgga gcgccttgat ttttttcgcg cccagcccgg
     5401 gcagcgagaa aagctcgaga atcccgggcg ggaatttcgc gcgcagctcg tcgtaaaact
     5461 tcgacgcgcc cgtcaaggca agctcggtaa tcttgccggc gatcgctttg ccgacgcccg
     5521 ggatttcctc gagccgattt tcctccgcca gcgcacgcag attgccgccc caggtttcga
     5581 tcgagcgcgc ggcgttgctg taggcgcgga ttttgaaggg gttctcctcc tgcagctcga
     5641 gcaaggtcgc gatcgtttcg agcacttcga cgatctgctc ttttttcatc gagggcacac
     5701 gaaagtcttg cgcggaaaat ccgactcgtc gataacgatg gtgcggaacg cgttgcgccg
     5761 ggcgcgtctg gcatggcaac agctttgcaa attctgatgt tcaccagttt tcgtttcgcg
     5821 ctttttttcg gcttcctcct ttttggaatc attccacgcg gactggcgaa tccgtttccg
     5881 aggaatggcc cggtcgtccc gggcagggtt gcgcgcctgc gacgcgggat tgcctacgcg
     5941 ccggcgaagg cgccgctacc ggtaaagcgt gcgatctggg cggccaacga actgcgctcc
     6001 aagccgtatc gctggggcgg cggccatgcg tcatttttcg acatcggcta cgattgttcc
     6061 ggaaccgttt cctatgcgct ggcggccgcg ggcttgctcc gggccccgct ttgttccaac
     6121 gagttgactc gttttggcga ccgcggccag ggaaaatgga tcacggtcta cgcccgcaat
     6181 ggccatgcct tcgccgtcat cgccggcttg cggctcgata ccacggcctg gaacggatgg
     6241 ggcgatcgcg acgcaccccg ctggcaactg acgctgcgcc cgccgcgcgg atttgagccg
     6301 cgtcacccgg acggacttta gtttcagaaa acaaaacatc cggccgaggt ggggccggat
     6361 gtctattcac ctaacgactc gcggaattgg tgacgaacgt tcgggtgaat ccgccgtgcg
     6421 gggttggacc gaaaagcggc tgatcccgtt gggctgcgct tattgcaagg tgcggcccgc
     6481 gatcgtccgg cccgcgactt ggcggcccgc gacggtatgc ccggcgacct ggcggccggc
     6541 aatgtggact ccacccttgg cgtctttcgc cggtttgagg tcctgcactt tcacttcttt
     6601 tttcttctta tcagtagcca tactatctat ttccttcgtt gttttgttgt tggtttggtt
     6661 ggttgattaa agcttcggtg aatcggccgc ccgggctgga acccaaggcg gctgatcccg
     6721 ttgggctgcg cttattgcaa ggtgcggccc gcgatcgtgc ggcccgcgac ttggcggccc
     6781 gcgacggtat gtccggcgac ctggcggccg gcaatgccga ctccaccctt ggcgtctttc
     6841 gccggtttga ggtcctgcac tttcactgtc tttttcttct tatcagtagc catactatta
     6901 tctatctcct tagttgtttt gttgttggtt ttggttggtt gattaaatct ccggtggatc
     6961 ggccgcccgg gctggaaccc aaaagcggct gatcccgttg gactgcgctt attgcacagt
     7021 gcggcccgca acgtagcggt gatcggcaac cggcccgcgc tgccggaccg gcgccctgac
     7081 tccgcccttg gcgtctttgg ccggcttcat gtcttgcatt ttcactgcct ttttcttctt
     7141 attagtagcc atactatcta tctccccttt ttgttgttgg tttggttgat gtcagtcgca
     7201 ccgaaaagca attgacggcc gcccgagacg ttccctttcg ggatgagatc atcgagcctg
     7261 atcaattttt cttttttgcg cttctgctga tgaatcggtg atccaccggc aggtgcattt
     7321 tttttgtcct ttggtgagct cacgtcgaca gcctattaac accaatcatg ccaacaccag
     7381 tctggagtga aaaatccgac cgtttcggag tcgggttcca agccagattt ccctcgcgaa
     7441 aacgtatttc tgccctctcc aaaattttcc ctaacgagtc gtgcagccag gctcgtccat
     7501 gggaaaagac cggccccgat gcgaggctat ctggtccagt cttatcccca gcccggctca
     7561 tttttgaacc ttgccccgaa gatgtcgagc cagcgtcgcg cgcgagatgc cgagcaaccg
     7621 ggcggtgcgc cgctggttcc ggccggcttg atcgtaaaca aatctcacat gccgggcgac
     7681 ggcgccggcc agcgaaagat cgcgcacccc atcggcctgg ccattgggcc acggagccgc
     7741 ctccgccacc ggtgtcggcg gcggcgagct ggatttcaag tcgggcggaa agatcacgtg
     7801 ttcgggcagg atgacgcggc cttcgcagaa gagcgtcatg gtccggacgg tgtggctgag
     7861 ctcgcgaaga tttccgggcc agggataagc cagcaactta tccatcgcct cgggatgaat
     7921 agcggtgatg gttttcccct cgttaaaggc ggaggctttc agttccgccg cgattaaggc
     7981 cgggagttcc tcgattcgtt cgcgcagagg cggaataagc agggtcaaac cgttcaggcg
     8041 atgataaaga tcctcgcgaa acttcccctc gcggacgcgt tcccggagcg agcaattggt
     8101 ggcgcagatg atgcgggtgt tgcaggtgag catctgttcg gaaccgagcc gctcgaattc
     8161 gccatactcg aggacgtgca gaatcttgac ctgcgcgagc ggactcatct cggagatttc
     8221 gtcgagtaac agggtgccgt cgttagagag ttcgaacttc cctttcaccg tttgttgcgc
     8281 gccggtgaag gcaccgcgtt cgtggccgaa gagctgactt tcaagcaagg tgtcgctcat
     8341 cgcggaagcg ttgaaggcga tgaacggttt gttcgcccgc gcggaagaat tgtggatcgc
     8401 gtgggcaaga agcgttttgc cggtgccggt ctccccgagg aggacgaccg gcactgccga
     8461 tttcgcggcc agcgcggcca ggcgaaggca gcgcttcatc gcctcgttcg cggtcgtcag
     8521 gtcctcaact ttggctctgg aaagcggacg cggcatgaca aatcagtctc gcagattttc
     8581 gcgcagctgc cggatcggct caaacgcgta ctccatgatg gtgcgctgcc ccacgacgat
     8641 gcgcgcttcc ccgcccatcc cgacccgcaa agggagagga tggcggcgcg ccgccagttc
     8701 cttttcatcg agcgaggcga gcgccatgaa atgcgggccc gcgagcgaag tgacggcgga
     8761 cgggctgacc caatcgagtt ttgcattgac cacgccgtaa cgctggtaag gaaaggcgtc
     8821 gaaaaagaag cggacgcgct gcccgacggc gagtcgcggc accgccgttt cgctgagggt
     8881 gagccgcccg cggggtttcg ccgcgatcgc ggccagctgg caaagttcct gtcccatttg
     8941 caccacgctg ccggcgttct gattggccac tgaaatcacg acggcggcgt agggcgcccg
     9001 gacggaaagc aggttttgct gcgaattctc gaggtcgcct ttgagcgcgc ccagccggac
     9061 cttgagcttg gcgatctccg ccatattttc atcgtgcact cgcgcatgct cgctctccag
     9121 ctgctgccgt tcgagtttga cctgctgcaa agtgcgttgt gcgacgctca ggtccttgtc
     9181 cgattcggcc gcttggagcc gatattgaat cacttcgacc agcgagatgc cgccattctt
     9241 ggtcagtttt tccatgcggt tgagcaactc ccggctgctc gccgcatgct tctcgcggaa
     9301 ccctacttcg ctctccgcct gcgagatttc cgccttcttg atctccacct gcgcggcgtt
     9361 ggcggcgtcg gctctggcca atccctcctc gtgcgtccgc aggtcggctg acaaggtgcc
     9421 cagctgcgtc ccccaatcac gaatctcgtc cgagcgcaga acaaagagcg gatcgcccgc
     9481 tgccaccgtc tggccttcgg tgacgcagac ccgattaaca accgccatcc gtgacgcctg
     9541 gatcgggtcc gcgccgccgg tcggcacgag gatgaaggga caggtgaccg tctccggcag
     9601 tttgatgagg atggaagcga gcaaagcgac cccgaacatc gcgatgaaca gccaggcggt
     9661 ggagcggacg aaccagggcg gcggatcctg cggcaacatt tcggactccg tttccccgga
     9721 gccacgctgc atgagttggt taggcattgt taggccaatc tcaaatcccg agctgctggc
     9781 tggagaggta aaaataaagg ccgcgctgcg ccatcaactg gtcgtggttg ccgacctcgg
     9841 cgaccattcc tttttccagg acgacgatcg agtcggcctc gcgaatcgtg ctcagccgat
     9901 gcgcgatcac aatcgtcgtg cgccccgccg tcaggcgacc gaggttatcc tggatcgcgc
     9961 gctccgattc cgtgtcgagc gcgctcgtcg cctcgtcgaa aatcaggacg ggcgggttgt
    10021 tgtaaatggc gcgcgcgatc gcgatccgct gcttttgccc gccggaaagc gcgaggccgg
    10081 attcgccgat tttcgtttcg tagcctaacg gcagccgcat gacgaaatcg tgggccgccg
    10141 cggcctgcgc cgcggccagg acgcgatcga ggtcaggctc ggcgtcgcca aaggcaatat
    10201 tgcgcgcgat cgattcgttg aagatgtggt tctcctgcag gaccatcccg atgtgccggc
    10261 ggacgtcgcg atagttgagc gttttcaggt cggtttgatc gaacaggatc gtgccttcgg
    10321 tgggctcgag cagacccgcg atcagcttga tcagggtggt ttttccacaa ccgctccggc
    10381 cgacgaaagc aaccattcgc ccgggcgcga tgtcgagcgt gatgtccgtc aggatgttgg
    10441 gcgcctccgg tccgccgtat ttaaagctca cgccacgcat ttcaatccgg ccctcgagac
    10501 tgtgcaccgg gatgagctgg gaacggtcgc gtccctgttc cggttcctgc tcgaaaatat
    10561 cgttcagccg attgagcaaa acagtcgcga actgcatgtt atcccacacg cctaacgttc
    10621 gcaggatgcc gctgtaagcc atcgcagtga gcgagctgaa agcgacgaag ccgccgatgc
    10681 tgagcgcgcc cgacatcacc tggttggccc cgacccagag aaagatcgca gtcgaaatca
    10741 tgccaatggt ttgcagcacg ctgtcgtagg tcatgacgat gaagctgctc cggaaaatct
    10801 tgcgcgagac ggagaggaat tcgttcaaca tcatgtcgcg gaagacgttt tccgcggaag
    10861 cggccttgac cgcttcgatc cctttgatcg catcgatttg atgggagctg tacttgccct
    10921 ggctctcctc cactccggcg aagagtgggc gcagcacctt ttgcgagaaa tacatgagcc
    10981 cgccatagag cggcgtggtc gcgaggaagg cgaacatgag cgacgggctg tagagcgcca
    11041 tgagcgaaat cgcgccgacg agctgcacaa gggcgaggag cgcgccgatc ccatgctgca
    11101 cggcgaactg tcggacctgc cgtgcgccat cgagccggcg ctggatgtcg ccggtgcgtc
    11161 ggctggtgaa ataagtcatc ggcagcgaga gcatttgccg gctcagataa tcgaggatcg
    11221 cggtgtcgag ccggacggct gcgaaggaga gcaaatattc ctgggcgagt gacgaggcct
    11281 ggacgaaaaa gaccgccgcg cccatcccga gaagaatggt tttgagcaag ctgatgttct
    11341 tttcgacgat caccttgtcg accaccatct cggtgaagat cgggaagagc agctcgagga
    11401 aggtgatcgc gatcgcgagc agcatcacct ggagaaaaag ggtcttgaac ttcgccagga
    11461 acggcaggag ccaggcgaac cccggtttgc tttctggggc gctttcgaag gcgaccgtgt
    11521 aatcgaaaag cgcggcgtag ccggaccaat tctgctcgaa ttccttccgc ggaatcttgc
    11581 gtaaaccgct cgcgggatcg ccgactctta cataggtgtc ggtcacgtcg tagaggacca
    11641 tccagtggtt gccttcccaa tggatgatgg ccggcagcgg catgaacggg agattgcgca
    11701 ccgacacctt caacgcgcgc gccgccaggc cgagctccgt cgcggcgcga cagagcgcct
    11761 tcaggctcgt cccgtcggtc gaggtgtgac aaagctgacg aatccgcgaa agactcactt
    11821 tgcgtccgaa gtggcggcaa atcatgccga ggctggccgc gccgcaatcc atctcgtcga
    11881 tctgctcgat gtgctcgagg cgcttgatcc gtcccttctt tttccggaag aggccgctct
    11941 cgtctgcgaa tgggtcctcg ccctcgaccg ccgcgggcgc cggagcggcc ttgtcgccat
    12001 cgaggccgat cttgtcgtgc gccgccactt cggccggcag catttcggtc gtgaaatcaa
    12061 gcggcacgcg cgcttccgtt tttacctggt attgcgccag ccgttcttcc atcagtttcc
    12121 cgaattccgg ataacggcgc ttcaagtcac ggaccgcatc cggctcgagg gcgagcagac
    12181 ggcagtcgct gaacgcttcg accgacgcgg cgcgcggcga gccgttgagg atcgagagtt
    12241 cgccgaagaa atcgccgtcc cggtaaaagg cgaggttgtg ttccgttccg ttgtcggaat
    12301 ggaaagcgcg cgcgcgtccc ttctcgagaa tgaacatcgc gccgggcgcg tcgccctgcc
    12361 ggatgatcgc atcgcccttt gcgacttcga ctggcgtcag tttttccacc agactgcgca
    12421 gcgcggggac ggggagccgg ccgaagttgc tgaactcgta aagaaaaccg tgcagcgccc
    12481 gatgtcgcgc ggtcatctcg atataatgtt tcagctccgg tgtttcctcg gccagctgga
    12541 gaaaatcggc gcgatccagg cgcaacgcct cgaccgccgt actgcaacgc accgtggcgt
    12601 tgcgggtgcc gccttcggaa agggcggctt cgccaaaact gtcgccgggc ttgagcgtgg
    12661 cgagcgcgat ctcattgccg ctttgatcgg ccttgaccac ccgggcccgg cccttgagca
    12721 ggacgtaaaa cgaatcggcc gggtcgcctt gcctaacgag aaggtcgcca aactcgtaat
    12781 tctcctcctg caggagcggc cgcagttttt cgaaatgctc ctccggcaaa aattggaaga
    12841 gcgaggaacg gcgcagaaga ttttcgtcgt ccgctttcat ttcgttaggc aagcagaatt
    12901 tgccacagga tgtagcgcgt cgtcaggtcc gcaaaatggc ggtcgaggat acggttgtcc
    12961 gcccgctctt gcacgatcgg atcgtccacg tccggttccg ctttgccgat gatccggcag
    13021 agatggaaac cgtcgccatg cgcgatcggt tcgagaatgt cgcccgggtg cacgctgaga
    13081 aatttctgct gcaaatcgtc tgggatttcc tcgagtagca cttcggtgtg gcggtaggga
    13141 taacgtcctt cgccggcgac ttcctccatc gacatgccat cttcgcgcgc gcaaaggagc
    13201 gcttcgcgcg cggcatcgag cgaatccaat tcgatggtct ccacctcgaa gcgggtcaac
    13261 ggcaggcgga gggccgcgat ctcgtgttcc cgggcctggc gcgagagaag cgcggcgcag
    13321 tcgcggcgct ggatcgcttc catcgccagg gcttcgcacc accaatccct gtcgcgctcg
    13381 agctgctcca gccaggcggc cagttgatcc tcactcaggc ctctgcgctc gaaaaagagc
    13441 gcttcctccg cggcgatcag ttgtggatca atggcctggc cttgcgccgc gcaacgggcg
    13501 gcgacgcgcc ggctcaaccg ttcggccatt cgatccagct cccccgagag gatcaattcg
    13561 cgggtcagca gctcgcgcag gtcgctcggc gcagagaaca aatcgatcgc ctccggttcg
    13621 atctcgtccc attgctcgcc ccaataatgc cggacaaaat aagcgctaaa atcgctcaag
    13681 gcgaggccgc gctccgccag ccaatgctcg gtttcctccg cggtgatcag atcgtgctgg
    13741 tagcgaaatt gttccgccgc cgtgtcgatc acctcatcgt tgaattccag atcctgctcg
    13801 cccgcttttt tttcggccgc gacgcaacgc aggaggttcg tccattccgc ctccagctcg
    13861 ccgcggaaat gggcggcgtc gatcacgtcg cgcacgctaa agtcccggtc tccgcaggtg
    13921 aaaatgattt tcttctcgat cggcgttgtt tgcgtctcag gcatcgggac ttgctcccct
    13981 gattaccgga caaattagca cccgtgtcga gcgaaaccac cgttcttgtc aggcttggcg
    14041 cggcttccct tcttcgcggc ttcctgttat ttgaggcttg gtgagcgttc atcgagcatt
    14101 atggaaaccc cgctgagtcc gctcgagttc gcgcggcgca cccgccggat ctatcccggg
    14161 cgcgaggccg tggtggcggg cgagcggcgg ctgaactacg aggaattttt cagccga
//
LOCUS       DATMNS010000174         4267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1671332, whole genome shotgun sequence.
ACCESSION   DATMNS010000174 DATMNS010000000
VERSION     DATMNS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1671332"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>4267
                     /locus_tag="VNW28_05970"
     CDS             <1..>4267
                     /locus_tag="VNW28_05970"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HXA09509.1"
                     /translation="DRRDLEKHIALSMIGGSNVFADKPAPFFAVTYGLHDRLAYVRSV
                     PLALPEHEDFMKIVVSKGVATTQGGAQTASDVDDKVRVPDAFSFFKIDESNARVVRNA
                     DGDPEQVLIVSTSAAAKSDEIRKALHVYLLPKKPSTKEEEAATADESPSENDSENSDE
                     DSSEATATETPSGWQSPREVNDEVLKNAKPVALSVIPSEHEQTQTHAFKFSVEEEGSL
                     YIRIDKGVKAVGGFPLPETYDAIVAVPTLPREIQIQGNGGVLALHGERKLSIKSRGIG
                     EIEYEIARVAANQINHLVTQTNGSFENPEFFGEFDEENISRIAVERQAINLENRFRPN
                     YSAFDFSSHLQLPTDGGSERGLFFVRAREWDPVKKRAVRDVSDRRFILVTDIGLLVKK
                     NADGSSDVFVLSIKAGTPLAGVAVQILGKNGVPLVSGTSSPDGRVNFPSLGKPAHEKR
                     PVAFVARLGDDVSFMPYARSDRALDFSRFDTDGVENVSGDQLEAFVFTERGVYRPGDE
                     VHIGCIVKQRSWQGQLEGMPVDVEIFDARDLRAQVRQLRLPAMGFAELNFQTANESPT
                     GAYRIEVYLLRNGKKDALLGSGDFHVEEFLPDRMKIESRLSQEAPRGWIDPKDVQAFV
                     TLRNLYGTPASNRRIASQLELAPRNFSFDEFKGYTFHDPLRDGKEERSGQTVDLGDQT
                     TDAEGKATIALNLERFADATYEMTFSTQGFEAEGGRSVHAYNSLLVSALPRVIGYKPD
                     APLDYLPKDSAHSIDFIAIDRALNKVATEKLQFDLIEQTYVSILAKKDNGNYAYESVL
                     KERLVKSEAISIDAPGLRYSVPTGAPGNYVLQIHDESGRVWNKLSFSVVGLGEVSRAL
                     DKNSELQVKLNRSSCNAGDEIEIAMTAPYTGSGLITIESDKVYAHQWFTSSTTSSVQH
                     IRVPEGFDGTGYVNVSFVRALDSKEIFMSPLSYAVVPLTVNKAKRQLRVDLRSSDLAK
                     PGEPLKIAYKTDRPARIVLFAVDKGILQVTDFKTPDPLGYFFRKTALGVETAQIVDLI
                     LPEFSILRAASAAGGDGEAEQRLNPFQRVTDKPVVFWSGIVDADTTEREVTYDLPDYF
                     DGTLTIMAVAFASDSAGSSEKESTIRGPFVITPSVPTMVAPNDKFEVGVTVANNIAGS
                     GANAAVTLTAEPSAQLEIVQGPAMPLHVAEGRESTVIFTVHVKDELGAASLAFHASAP
                     GGEATRRATLSVRPPVPFMTQVSSGRFTNGNAQVPVTRQMYTAFRQLNATASALPLGL
                     ARGLDFYLKNFPHGCTEQITSAAFARLVLADEADFGLSRAEVNEQLEKEFAMLRRRQS
                     DQGAFGYWSAETSPETDFVSVYATHFLSEAKAAGFAPPPDILQSALRNLQTMAARPPR
                     DLADARTIAYAIYVLTREGFITTNYILNLRDYLDKQFEKQWPQD"
BASE COUNT          877 a         1311 c         1282 g          797 t
ORIGIN      
        1 cgatcgcaga gatctcgaga aacatatcgc cctttccatg atcggcggct cgaacgtttt
       61 tgcggataag ccggcgccgt ttttcgccgt tacctacggg ctgcacgatc gcctcgctta
      121 tgtccgcagc gtgccgctcg cgttgccaga acacgaggat ttcatgaaaa tcgtcgtcag
      181 caagggggtc gcgaccacgc agggcggcgc gcagaccgcg agcgacgtgg acgacaaggt
      241 gcgcgtgccg gacgccttca gttttttcaa gatcgacgag agcaacgccc gcgtcgtgcg
      301 caatgcggat ggcgatccgg aacaagtcct gatcgtgagc acatccgcgg cggcgaaatc
      361 ggatgagata cgcaaggcgc tgcacgtcta tctcctcccg aaaaagcctt cgaccaagga
      421 ggaagaagcg gcgactgcgg acgaatcgcc cagtgaaaat gattcggaaa acagcgacga
      481 agattcgtcg gaagccaccg cgaccgagac tccgagcggc tggcaaagtc cgcgcgaagt
      541 gaacgacgaa gtcttgaaaa acgcgaagcc ggtcgccctt tccgtcattc cttcggagca
      601 cgaacaaacg cagacgcacg ccttcaaatt cagcgtcgaa gaggaaggca gcctctacat
      661 tcggatcgac aaaggggtca aggccgtcgg cggttttccg ttgcccgaga cgtatgacgc
      721 gatcgttgcc gtgcccactt tgccgcgcga aatccaaatt cagggtaacg gcggagtcct
      781 tgcgctccat ggcgaacgca aactctcgat caaatcgcgc ggcatcggcg agatcgaata
      841 cgaaatcgcc cgcgtcgccg cgaaccagat caatcatctc gtcacgcaga cgaacggcag
      901 tttcgagaac ccggaattct ttggcgagtt cgacgaggaa aacatttcgc gcatcgcggt
      961 cgagcgtcag gcgatcaatc tcgagaacag atttcggccg aactattccg ccttcgactt
     1021 ctccagtcat ctccagttgc cgactgacgg cgggagcgag cgcggcctct ttttcgtccg
     1081 cgcgcgcgaa tgggatccgg tgaagaaaag agccgtgcgc gacgtgagcg atcgccgctt
     1141 cattctcgtc accgacattg gcctgctcgt gaaaaagaat gccgacggga gcagcgatgt
     1201 cttcgtcctt tcgatcaagg ccggcacgcc cctggccggc gtcgcggtgc aaatcctagg
     1261 caagaatggc gttccgctgg tcagcggcac gagcagcccg gatggccggg tcaactttcc
     1321 ctcgttaggg aaaccggccc acgagaaacg cccggtcgcg ttcgttgcgc ggctgggcga
     1381 cgacgtttcc tttatgccct acgcccgctc cgatcgagcg ctcgattttt cgcgcttcga
     1441 taccgatggg gtcgagaatg tttcgggcga tcagctcgag gccttcgttt ttaccgagcg
     1501 cggcgtctat cggccgggcg acgaagtgca catcggttgc atcgtgaagc agcgctcctg
     1561 gcaggggcag ctcgaaggaa tgccggtcga tgtggaaatt ttcgacgcgc gcgatctccg
     1621 ggcgcaagtg cggcagttgc gcctgcccgc catgggattt gcggaactga atttccaaac
     1681 ggcgaacgag tcgccgactg gcgcctaccg catcgaagtt tacctgctgc gcaacgggaa
     1741 gaaggacgcc cttcttggct cgggcgattt tcatgtcgag gagtttttgc ccgatcggat
     1801 gaagattgag tcgcgcctct cgcaggaagc gccgcgcggc tggatcgacc cgaaggacgt
     1861 gcaggcgttt gtcaccctgc gcaatctcta cggcacaccg gcgagcaatc gtcgcatcgc
     1921 gagccagctc gaactcgcgc cccgcaattt ttccttcgac gaattcaagg gttacacctt
     1981 tcacgatccg ttgcgggacg ggaaagaaga gcggtccggg caaacggtcg acctcggcga
     2041 tcagacgacc gacgccgagg gcaaggcgac gatcgcgctg aatcttgagc gcttcgccga
     2101 tgcgacctac gagatgacgt tttcgaccca gggcttcgaa gccgagggcg gccggagcgt
     2161 gcacgcctac aattcgctcc tcgtctcggc cttgccgcgc gtcatcggtt acaagcccga
     2221 cgcgccgctc gattacctcc cgaaagacag cgcgcacagc atcgacttta tcgccatcga
     2281 tcgcgcgctg aacaaagtcg cgaccgagaa attgcagttc gatctcatcg agcaaaccta
     2341 cgtttcaatt ttggcgaaga aggataacgg caactacgcc tacgaatcgg tcttaaaaga
     2401 gcggctggta aaatcggagg cgatcagcat cgacgctccc gggctcaggt attccgtccc
     2461 gaccggcgcg ccgggaaatt acgtcctcca gattcacgac gagagcggcc gcgtctggaa
     2521 caagctttcg ttttccgtcg tcggcctggg cgaagtctcc cgcgcgctcg acaagaattc
     2581 cgagctccag gtgaaactca atcgatcgag ctgcaacgcg ggcgacgaaa tcgaaatcgc
     2641 gatgacagcg ccctacaccg gcagcggcct gatcacgatc gagagcgaca aggtctacgc
     2701 gcatcagtgg ttcaccagca gcacgacgag cagcgtgcaa cacatccgtg tgccggaagg
     2761 tttcgacggc actggttacg tcaatgtctc cttcgtccgc gcgcttgatt cgaaggaaat
     2821 tttcatgagc ccgctgagct acgccgtcgt gccgctgacc gtgaacaagg cgaagcgcca
     2881 actgcgcgtc gatctccgtt cgagcgatct cgcgaaaccg ggcgagccgc tcaagatcgc
     2941 ttacaaaacg gatcggccgg cgaggatcgt cctcttcgcg gtcgacaagg gcatcctgca
     3001 ggtgactgat ttcaaaacgc ccgatccgtt aggctatttc ttccgcaaga cggcgctcgg
     3061 ggtcgagacc gcacagattg tcgatctcat tctgcccgag ttctccatcc tgcgggcggc
     3121 gagcgcagcc ggcggcgatg gcgaggccga gcagcgcctg aatccgtttc agcgggtgac
     3181 tgacaagccg gtcgttttct ggtcggggat cgtcgatgcc gacacgacgg aacgcgaagt
     3241 gacctacgat ctgcccgatt atttcgacgg cactctcacc attatggccg tcgccttcgc
     3301 ctctgattca gcgggttcga gcgaaaagga atcgaccatt cgcggtccgt tcgtcatcac
     3361 gccgagcgtg ccgacgatgg tcgcgcctaa cgacaagttt gaggtcggcg tgacggtggc
     3421 gaacaatatc gccggctcgg gcgcaaacgc cgcggtcacg ttgacggccg agccgtcggc
     3481 gcaattggaa atcgtgcagg gtcccgcgat gccgctgcat gtcgcggagg ggcgcgaaag
     3541 caccgtcatc ttcaccgtcc atgtgaaaga cgaactcggc gccgccagcc tcgcgttcca
     3601 cgcctccgca ccgggcggcg aggcgacgcg gcgcgcgact ctgagcgtgc ggccgccggt
     3661 gccgttcatg acgcaggtga gcagcggccg tttcaccaac ggcaacgcgc aggtgccggt
     3721 cacgcgccag atgtatacgg cgtttcggca gttgaacgcg accgcttcgg cccttccgct
     3781 cggcctcgcg cgcggtctcg atttttatct gaagaacttc ccgcacggct gcaccgaaca
     3841 gatcaccagc gccgctttcg cccgtctcgt tctcgctgat gaggctgact tcggattgag
     3901 ccgggccgag gtgaacgagc agctcgagaa agaattcgcg atgttgcgcc gccggcaaag
     3961 cgaccagggt gcattcggct actggtcggc tgagaccagc ccggaaactg atttcgtttc
     4021 cgtttatgcg acgcacttcc tgagtgaagc gaaggctgcc ggcttcgcgc cgccgccgga
     4081 tattctccag agcgccttgc gcaatttgca aacgatggcc gcgcgcccgc cgcgcgatct
     4141 ggccgatgcg cgcacgattg cctatgccat ttatgtcctc acgcgcgaag gtttcatcac
     4201 caccaattac atcctcaatc tgcgcgatta cctcgacaaa caattcgaga aacaatggcc
     4261 gcaggat
//
LOCUS       DATMNS010000175         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2986691, whole genome shotgun sequence.
ACCESSION   DATMNS010000175 DATMNS010000000
VERSION     DATMNS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2986691"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VNW28_05975"
     CDS             complement(<1..221)
                     /locus_tag="VNW28_05975"
                     /inference="COORDINATES: protein motif:HMM:NF014130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0030490 - maturation of SSU-rRNA [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HXA09510.1"
                     /translation="MTTVNHRRERIGEEIAHEINAMLAGELKDPRLEISVVVNEVRLA
                     PDGRNARVYVGARGTEAEQKGAIKALEHAA"
     gene            complement(339..620)
                     /locus_tag="VNW28_05980"
     CDS             complement(339..620)
                     /locus_tag="VNW28_05980"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HXA09511.1"
                     /translation="MPVGLLLLDLQLPDAHSLKDKRQILRSLKARLRRKFNIAIAELD
                     FHDVWQSSTIGIVTLSNAEQHVEESLQTVLREAQRILGPILVDHSVDFL"
     gene            complement(691..2685)
                     /gene="infB"
                     /locus_tag="VNW28_05985"
     CDS             complement(691..2685)
                     /gene="infB"
                     /locus_tag="VNW28_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXA09512.1"
                     /translation="MLPAPPLDKMPSKAEPGKPLYTRRPTQRQRPSSDKREQEGERKL
                     HPTRQRPGAGDRRSMAVIAPPEPRAPRDIQITEGITIRELAEKLDVRAKELLKVLLDK
                     GIFASINQALDSPTATQLAESFNGVVSVVSFEDEMMLEVAKEETKENLKPRPPVVTVM
                     GHVDHGKTSLLDAIRSEDVAAGEAGGITQHIGAYKIQVNDRAVVFVDTPGHEAFTRMR
                     ARGAKVTDIVVLVVAADDGVMPQTKEAIDHAKAANVPIIVAINKIDKPEAQVERVKRQ
                     LSDLGLMPAEWGGDTEFVEVSAKQKKGLDRLLEIILLVADLRELKANPDAPGAGTVLE
                     SRVDKGRGPVATILIQNGTLKAGDFFICGAVFGKVRAMFDDHGRPLKEATPSTPVEVL
                     GLQGVPEAGDHFQVTDEAKARHIVDYRQGKQRDAQLRASSGGRITLDQLHEQLKTGEV
                     KELNIVIKADVQGSVEVLSEMLPKLSTDQVKLKILGSGVGAVSENDVLLASASGAIII
                     AFGVRPERKAADLAQQEGVDIRTHNIIYEVSDEIKKAMEGLLEPVFQESYLGRAEVRN
                     TFRVKGAGTIAGCYVVDGILKRDAQIRVVRDGAVIYTSKLSSLKRFKDDASEVRTGFE
                     CGVGIANFNDVKIGDVLEAFNVQKLSGAEAAAQAGSSARK"
     gene            3252..3440
                     /locus_tag="VNW28_05990"
     CDS             3252..3440
                     /locus_tag="VNW28_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09513.1"
                     /translation="MKFVAEQPGLPREEQVLAPEHAARKREQQERLALRQRELEWRRP
                     EPEGALPVRPEPGEAWQA"
     gene            complement(3715..>4039)
                     /locus_tag="VNW28_05995"
     CDS             complement(3715..>4039)
                     /locus_tag="VNW28_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09514.1"
                     /translation="EQLMEIPGIGEKMVDKIYQAVNQFYEGGGVVAAAEGTPETTEAS
                     AEIGEAESPTESESTETESPATETEEEASVEAAPAESPAEQVADAAPAAEPEKVPEKH
                     DEENA"
BASE COUNT          694 a         1057 c         1432 g          856 t
ORIGIN      
        1 gcggcgtgtt cgagggcctt gatggcacct ttttgttcgg cttcggtgcc gcgcgcaccg
       61 acatagaccc gggcgttacg gccatccgga gcgaggcgga cttcgttgac cacgaccgag
      121 atttcgaggc gcgggtcctt gagttcgccg gccagcatag cgttgatttc gtgggcgatt
      181 tcctcgccga tgcgttcgcg acggtggttg acggtggtca tggtctttcg atcagctgca
      241 aaatgctttt gtacagtata ggagcgggaa gtacaagccg cgcagcctga gccagtacaa
      301 aagtgcaaga gtacaaaagt ataagcgata cgccgcagtc acaaaaagtc gacggagtgg
      361 tccacgagga tggggccgag gatgcgttgg gcttcgcgca gaacggtctg tagggattct
      421 tcgacgtgct gttcggcgtt ggagagggta acgatgccga tggtggagga ttgccagacg
      481 tcgtggaagt cgagttcggc gatggcgatg ttgaatttgc ggcggaggcg ggctttgagg
      541 ctgcggagga tttggcgctt gtccttgagg gagtgggcgt cggggagttg caggtcgagg
      601 agtaggagac ctacgggcat ggggagagtt taagagtcta agagttcaaa agtcgaagca
      661 cggcagcggg gtcgccgcac ttcacgtgat ttatttgcgg gcggaggagc cggcttgggc
      721 ggcggcttct gcgccggaca gtttctggac gttgaaagct tcgagcacgt cgccgatttt
      781 tacgtcgttg aaattggcga tgcctacgcc gcattcgaag ccggtgcgta cttcgctggc
      841 gtcgtccttg aagcgtttca aagagctgag cttcgaggtg tagatgacgg cgccatcgcg
      901 aacgactctg atttgcgcgt cacgcttgag aatgccatcc acgacgtagc agccggcgat
      961 ggtgccagcg cccttgacgc ggaaagtgtt gcggacttcg gcgcggccga gatagctctc
     1021 ctggaagacc ggttcgagca ggccttccat ggctttctta atttcgtcgg aaacttcgta
     1081 aatgatgttg tgggtccgaa tgtccacgcc ctcttgctgg gcaaggtcgg cggctttgcg
     1141 ttcggggcga acaccgaagg caatgatgat ggcgccggaa gcggaagcga ggaggacgtc
     1201 gttttcgctg accgcgccga caccggagcc gagaatcttg agcttgacct gatcggtgga
     1261 aagcttcggc aacatttcgc tgagaacttc gaccgagccc tgcacgtcgg ccttgatgac
     1321 gatgttgagt tccttgactt cgccggtttt cagttgctcg tgcaactgat cgagcgtgat
     1381 gcgtccgccg gagctggcac ggagctgcgc atcgcgctgt ttgccctggc gatagtccac
     1441 gatgtggcgg gccttggctt cgtcagtgac ctggaagtga tcgccggctt cgggaacgcc
     1501 ctgcagcccg agaacttcaa cgggagtgga cggagtggct tccttgagcg ggcgtccgtg
     1561 atcgtcaaac atcgcgcgga ccttgccgaa aaccgcgccg cagatgaaga agtcgccggc
     1621 ctttagcgtg ccgttttgaa tgaggatggt agcgacgggg ccgcggccct tgtccacgcg
     1681 ggattcaaga acagtaccgg caccgggggc gtcgggattg gctttcagtt cgcgcagatc
     1741 ggcgaccagc aaaataattt cgagcaaacg gtcgaggcct tttttctgct tggcggagac
     1801 ttcaacgaat tcggtgtcgc cgccccactc ggcaggcatc aggccgagat cgctgagttg
     1861 acgcttgacg cgttcgacct gcgcttcggg cttatcaatc ttgttgatgg ccacgatgat
     1921 gggaacgttg gcggccttgg cgtggtcgat ggcttccttg gtttgcggca ttacgccgtc
     1981 atcggcggcg acgacgagga ccacgatgtc ggtaactttc gcgccgcgcg cgcgcatgcg
     2041 ggtgaaggct tcgtgaccgg gtgtgtcaac gaaaacgacc gcacggtcgt tgacctgaat
     2101 tttgtacgcg ccgatatgtt gagtgatgcc gcctgcttcg cccgcggcaa catcttcgga
     2161 gcgaatggcg tcgagcaaac tggttttgcc gtggtcgacg tggcccatga cggtgaccac
     2221 cggcggacgc ggcttgagat tctccttggt ttcttccttg gcgacttcca acatcatttc
     2281 gtcttcgaac gaaacgacgg agaccacgcc gttgaaagac tcggcgagct gggtggcagt
     2341 gggtgaatca agagcctggt tgatgctggc gaaaatgcct ttgtcgagaa ggactttcag
     2401 aagttctttc gcacgaacgt cgagcttttc ggcgagttcg cgaatggtga tgccttcggt
     2461 gatctgaata tcgcgcggcg cgcgaggctc gggcggagcg atgaccgcca tggagcggcg
     2521 atcacccgcg ccggggcgct ggcgagtggg gtgaagcttg cgctcgcctt cttgctcgcg
     2581 cttatcggaa ctcggacgct ggcgctgagt tgggcggcgc gtatagagcg gcttgccggg
     2641 ctcggccttg gacggcattt tgtcgagcgg cggtgccgga agcatgccgg ggcgaccggg
     2701 ctgacgaggt ccgggacgcg caccactggg aggtgcgccg ggacgtggtc caaaagaacg
     2761 gccttgcggg ccgcctggac caccaggtcc agtaggacgc ggcgaatacg gacgaccttg
     2821 accaccttgg ccgccttgct gcggacgcat cggctggccg ggacgcgggc cactgggccg
     2881 atcaccggaa ggcagagggc cacgattgcc gctgggcatg ggtccgcgat taccagtggg
     2941 aagtgggcct cgattgccgg taggcaaagg acgttgaggc tgagcgccgg gccgagcagg
     3001 cgcacccgcc gcagggcgag cagatgccgg ctggcccggc cgttgctggc cggggagtgg
     3061 cggacgctgg gcgggagatc cacccggaat attttgtgaa gtggggcgca gcggctgtcc
     3121 ctggggagcg ggtggcacgg gccatgaagt ggaaggcgta tgagcacgcg gcgcagcggg
     3181 agtagcaggc ttggcggctg agggaggcgc cggagcttga gtagccgggg ccgaaggcgg
     3241 aggtgcggcg ggtgaaattt gtcgcggagc aaccggggct gccgcgggag gagcaggtgt
     3301 tggcgccgga gcatgcggcg cggaaacggg agcagcagga gcgattggct ttgcgacagc
     3361 gggagctgga gtggcggcga ccggagccgg agggagcgtt gccggtgcga ccggagccgg
     3421 gggaggcgtg gcaggcttaa gacgagcagc gcgtgcggcg gcgtccttgg cttgccgttc
     3481 ggcgacggcc gcggattgca cggctgcgtc ggcgtcggct tgtccctgaa tggtgttgcg
     3541 aactttttca gcgacgtcca ccgggatcga gctggagtga gtttttttct ccgcgacacc
     3601 gaagccgggc aaaaggtcga tgatggcctt tgctttgact tcgagctcgc gggccagctc
     3661 gttaattcga acttgattgg cgtcggtcat gctttccgtt cgggtcacct gttattacgc
     3721 attctcttcg tcgtgttttt ctggaacttt ttcaggttcg gcagctggtg cagcgtcggc
     3781 cacctgttcg gccggcgatt cagctggcgc ggcttcgacg gaagcttctt cttcggtttc
     3841 cgttgcgggt gattctgtct ccgtcgattc ggattcggtc ggggactcag cttcgccaat
     3901 ttcggcagat gcttcagttg tttcgggagt gccttccgcc gcagccacaa caccgccgcc
     3961 ttcgtaaaat tgatttacgg cctggtagat tttgtccacc attttttcgc cgatgccggg
     4021 aatttccatg agctgttcg
//
LOCUS       DATMNS010000176        23204 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3344394, whole genome shotgun sequence.
ACCESSION   DATMNS010000176 DATMNS010000000
VERSION     DATMNS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 23204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23204
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3344394"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VNW28_06000"
     CDS             <1..228
                     /locus_tag="VNW28_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09515.1"
                     /translation="ARAPKTDVIIDNIRLSPETFGIAAGVTVMWTADSRVQQSPVLKT
                     GQSFSDTVATAKNYSYLCAIQPRVTEKTIVR"
     gene            251..715
                     /locus_tag="VNW28_06005"
     CDS             251..715
                     /locus_tag="VNW28_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09516.1"
                     /translation="MKSALCLTRLKTALLVVPLSLYFCGPRMAEGRDRSLLPPGVVRP
                     GNRDYLARSTEGSLTVYVTGDEFNDAWYFPRSVYAIYTIDGKLFKSVESQYSADEEIP
                     DVVALPFGSYLVIARSEKKGYIRRPVVIEAGRRTILDLDLREEGSLRRIAHS"
     gene            754..1206
                     /locus_tag="VNW28_06010"
     CDS             754..1206
                     /locus_tag="VNW28_06010"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HXA09517.1"
                     /translation="MNPFDLKAALLAKHAQHPVIIHFPIALFIISVAFDLLAIWRRNP
                     AVAKAAYYNLLGAALTAPVAIASGLTAWQWQLEGAKLKGNLLLHLIFALSASGMIWLL
                     CGWRISQRKRPQPTPGLIYILLALATVLLIALAGHLGGFVSGVEGGPT"
     gene            1926..3107
                     /locus_tag="VNW28_06015"
     CDS             1926..3107
                     /locus_tag="VNW28_06015"
                     /inference="COORDINATES: protein motif:HMM:NF025049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXA09518.1"
                     /translation="MHSDTNHSDEDNTSTAAESSRRAISVPAPNRLARRAFLRNLGMG
                     AALLAPGATLLGNAGKALAAGNTKEDNALSRGDVAILQLLAAAELIEADLWQQYAELG
                     GVDSPTTGYTAGLQILDGDQLQYISDNTDDEQSHAAFLNAYLASKGESQVDLSQFKNL
                     APSKVSFVPQTGRLTNLKELTVDTSWWTRYRSTTNPDFGATFPDAVPSLNTGLHTAIP
                     RVDAELDDPNNPSDHIKAIAFTAGFHFGFIEQGGTSLYATLAQKVTSLEVLRILLSIG
                     GSEIMHFQTWQDKAGNATPLTDFDRINNSTVTFVDLTTGQPETLQANLIMPEPCEFIS
                     PNLPACAIIRPTGPGQLDATGAINSFISDGLFRGQPQTFLKLLSSLARAADAAHREIG
                     D"
     gene            3287..4252
                     /locus_tag="VNW28_06020"
     CDS             3287..4252
                     /locus_tag="VNW28_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXA09519.1"
                     /translation="MSDYISHDHEHDGIDRRGFLQCMAWAGTGLLWTISGGVLASKTL
                     AQIAQSGDSLSKATDLSFLQISDSHIGFSKEANKDVTATFRIALDRINAMPTPPSFLI
                     HTGDISQLSKPEEFDTFDEVLKSCRTKDVFYVPGEHDVLDGSGKLYRERYLKSVEGRG
                     SGWYSFNKNGVHFIGLVNVLNLQAGGLGQLGDEQLEWLEADVKGLASSTPIVVFAHIP
                     LWAVYPEWGWGTSDSERALGYLKRFGSVTVLNGHIHQVLQKVEGNVTFHSAMSTAFPQ
                     PAPGTAASPGPMKVPAEKLRSMLGLTSVKYLQGKSSLAIVDSNLS"
     gene            4266..4640
                     /locus_tag="VNW28_06025"
     CDS             4266..4640
                     /locus_tag="VNW28_06025"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HXA09520.1"
                     /translation="MYTSVTRNQTVRILFTISSLCLSSLSFGQAEKGASADNTKTNQI
                     VIKDFHFDPQVLTVRSGEKITWTNRDEEPHTVVSVEKQFKKSGPLDTDQEFTITAGAP
                     GTYTYYCSVHPKMTGTIVVKKS"
     gene            4718..5017
                     /locus_tag="VNW28_06030"
     CDS             4718..5017
                     /locus_tag="VNW28_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09521.1"
                     /translation="MKPTPDICNRRKSGTLAVSTSRVFPKTDFNYQATTLANLEGRWA
                     DLCKSSFRAISQDYFANEAPRHFAHEAGLFFMMVMTVTLPLLNASAAALGLIRIR"
     gene            5309..5842
                     /locus_tag="VNW28_06035"
     CDS             5309..5842
                     /locus_tag="VNW28_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09522.1"
                     /translation="MQCIVVAEFNAKPIREQNLSEWKRGGYRDWEAQQEAMGIAECLG
                     ADAAEWTGADRASLTDTLAHFLVARYAVATRRVAETGGREGWHLLREMGSAIVELRKG
                     DHSAKRLQIDRERLKLETEKSGETDPRKGRSAVETTGGEGRAHPGGASAANARNLRAG
                     TRRKRKRRDFLRNARGD"
     gene            complement(6056..6577)
                     /gene="uraD"
                     /locus_tag="VNW28_06040"
     CDS             complement(6056..6577)
                     /gene="uraD"
                     /locus_tag="VNW28_06040"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HXA09523.1"
                     /translation="MTKALTGNAMLTLDEVNAFDPEMFTVRIRPVFEHSPWVAARTAA
                     ARPFATREALHAALCRTVNQASDDEKLALIRAHPDLVNRAVLTHESEAELKSAGLDQL
                     SPEEIERFTDFNRRYREKFGFPFVICARRNKREAILTALPGRLQNSRAEEIKTALAEI
                     CQIAELRLRDLVT"
     gene            6983..7837
                     /locus_tag="VNW28_06045"
     CDS             6983..7837
                     /locus_tag="VNW28_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HXA09524.1"
                     /translation="MAAEDSDTGIKIYLREIGQIPLLTPLQEIELAAKIKKGDKEARA
                     LMIRSNLRLVVKIAHDYANLGLPLLDLVSEGNIGLMKAVERFDPAKGGKLSTYAAWWI
                     KQSIKRALANQSKTIRLPVHLVDKISKMRRVSLQMSEELGREPTDDELGEEIGIAAGK
                     VSQLKTVSIRPASLDAPISDDDSTEFGEIVGDEEAQTPFELLRDKNLRNEVGGLLDVL
                     DDRERKIIFSRFGLDGGKPKTLEEVGKKFGVTRERIRQLQNIALSKLRRALSKKERPV
                     DVELTAEV"
     gene            complement(7903..9309)
                     /gene="pyk"
                     /locus_tag="VNW28_06050"
     CDS             complement(7903..9309)
                     /gene="pyk"
                     /locus_tag="VNW28_06050"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004491.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HXA09525.1"
                     /translation="MRKTKIICTLGPATEKNEMLQELMLAGADVFRINMSHAEHAWVR
                     DIVPRARKFANDLEHPLAILLDTQGPAIRTGALAHAIELNPGDLLELTVRGAKGEAAY
                     SVDVNYDGLIDDVSLGNKILIDNGLLQLVVMKKGKNRILCKALTAGTLGSRRHINLPG
                     VHVNLPPLTNKDLRDVDLGVELAVDFVALSFARQTSDLEALRKVLAEKENKSTARIVA
                     KIEEQSAVKQIDEMIKTADATMVARGDLGIECPMEELPIIQRRIVKRSLRIGKPVIVA
                     THMLESMIVNPVPTRAEITDVANAVFEQADGIMLSGETSMGRYPVECVKVLDRVARRI
                     ERSGGAGFSRDAILENLRQKTVASAVVLANSLPNAKLIVFTRHGTMARYVANMRPEHA
                     PIFAFTPSESVYRQLALCWGTAPVRLDFTADPNGNITAAENYLRVQRLSRDGDNLVII
                     SDTQSGDAMVDCVQLRKA"
     gene            complement(9367..12183)
                     /locus_tag="VNW28_06055"
     CDS             complement(9367..12183)
                     /locus_tag="VNW28_06055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA09526.1"
                     /translation="MSRAAGATRPILLFSHETTLSGAPLALYYLACWLNEHGTPPVVA
                     APERGPISDLLVARGIETILDATFLTDTKREKLDALCADFETVVANTLASWQAIRAAR
                     AAGRCAIWYLHETLLAVELMQKISEIQPTLNLADLLITPTRQTARLYEGATRSRIEVV
                     PYGIPEIEAVARKMPNEETLKFFCLGSYEPRKGQDILLDAIARLEPELRRRTSFGFAG
                     RILDEDFFADLQSRAAPLENVHLGPALEHPASLQFLGQIDVLVCSSRDETMPISIIEA
                     MGLGKGVISANVGGIAEWLRDGLNGWLVPAEKPNELARAITKCAADPELRKFCGASAR
                     RTFVRHFALDRFGKRFQEAIEIASKSHRTIRKRDLHTYRQWVASYDTPGAGERIALRQ
                     QLGALRRWPRISVLLPIYNPELKVLATAIDSVKAQLYEKWELCLADDASTDPKVRPFL
                     EASAASDPRIKLTFRETNGHISAASNSALALTTGEWCALLDQDDEFAPHALALVALEM
                     DAHPEARVIYSDEDKIDLAGVRSNPFFKPDWNPELFHGQNFINHLGVYEAALLREIGG
                     FREGYEGSQDYDLAARCFDRIRADQVRHIPRILYHWRSVPGSLAALPNAKPYAREAAR
                     RTLRDHLTRKGIAGRVEPCPENNESHRVIYALPGPAPRVTIIIPTRDRIALLRRCVES
                     LRAVTDYPAFDILIVDNDSTENETLDYLRQLEAEGAASILREPGPFNFSRLNNRAARQ
                     ARSEILAFLNNDIEVSEAGWLTEMVSHATRAEVGAVGARLWYRDGTLQHGAVVLGLGG
                     VAGHAHHRVPAGHPGYFNRAILQQNCSAVTAACMLTRKAVFQELKGFNEPDLGINFND
                     IDYCLRVRARGWQVVWTPYANLVHDESGSRGHHEDPKQQAQFFREATYMQETHGAALL
                     HDPFYNPNLSLALPGYEIAFPPRS"
     gene            complement(12191..16171)
                     /locus_tag="VNW28_06060"
     CDS             complement(12191..16171)
                     /locus_tag="VNW28_06060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA09527.1"
                     /translation="MLPPLPSLGFQPRFFTSGVARFHLPFLYDLVALRRPRQVVTLGF
                     EDEQVHFTFCQAAREQKLTSHCLTIRRPRIGENAEDDVAWQGAIEKSEEFFHDWGTLR
                     ERDPREEGAQHEDGTVDLLLIVDCDSAESLRAELRVWRPKLAPQALVLLHGTLLERES
                     PPRAAWEEYCAGRPNYEFDEGIGLGVASLSAGVSADDSLCAALFGPAEGRRDLKQLYQ
                     AIAARIEAQDRVSEAERQKSALQLRQAWLDTLLQDRWKAQEVMDQLARDLAHLRGVID
                     AGKHERAFQDLHRDRIKAQLIMDAQAEQLKQWVGRSEALSAENKKLKTTVAEQKRILN
                     AAKKACKKKGRCFQASSDGKKKERRSIPQRVLRELHRIPANLGRLAKRKEVPLVEKSP
                     APETAIARYAKWIEQHEPDAAALEAQHADATALPVQPKISFLLPTHNTPTAFLDELLA
                     SLGAQSYGNFEICAVDGGSSAAATIECLTTWQKKEPRLRVEFLPENLGIAENTNRALA
                     LATGDFITCIDHDDRLPPFALYELARAIARHPGGEIFYSDEDRLSADGKRHSPFFKPE
                     WSPEYLLSSMYLGHLTLYRRDLVARVGQFRREFDLSQDYDFALRATEQAREICHVPHV
                     LYHWREHPASGSAGGKPEARKTNLAALAAAMERRGLAAEILEYPAANRVRLRISQWPK
                     VSIVIPTDSALRGQFCVEELPKMTSYPDYEVVIVTNSALAEQLEIIAPKKPAFRFVRY
                     DQAFNFSDKCNLGARAAAGERLIFVNDDVESGQPDWIQNLIEPLENPAVGAVAPKLLY
                     ESGKIQHAGLVTGVRGLLGTACHQWPADSPDYTNFAQSMRDVSALSAACLAMRSEDFF
                     SVGEFDAVNTPIAHSDLDLCFKIRAAGLRCVYTPFITMRHRGHASIGAVEHTATIPRR
                     EKASVFLLQRWPAYTCHDPYFPDNVRDWLYADSPTPIRMWARDQASAPAKKGDLLFVS
                     HDLTWSGAPLILLQLAQWCKEHGFFVTVMSPEEGPVRQKFFDAGIPLIVDPLVMEGHP
                     SFARFAREFDCVIASTIFGAPIVCAAKAAGIPHVWWIHEGRVAKHYLGSDARMRAALG
                     LADLIITPDTHSSQVYQPFTNRAIRVLNYGIPDPGAGLESTAEREIEQVRFLVLGTIE
                     QRKGQEVLLNALRQLPTEVLQHVHFQIVGRPHDLELTAEIKAAAREHSYLSYEESVTH
                     EQALALIRAADVMVSASWDETGPLILIEALALGTPILSTSVGAVAENLIGEEAGLFFP
                     PGNATALAEAIQRLVREPELIDRLRAKSRPTYEKYFTFDRFAEGFVELMNEVSHSNAA
                     AEVHSL"
     gene            complement(16383..17051)
                     /locus_tag="VNW28_06065"
     CDS             complement(16383..17051)
                     /locus_tag="VNW28_06065"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXA09528.1"
                     /translation="MRSDYKGTWTALSQTLSSAKMHVSGTEDEATIATSGVGTSDFLF
                     KNVGISPTDVVLEIGCGIGRVGQCLAGRCRRWIGADVSPHMLGFAAERLREFRNVELV
                     ELSGYDLRPIADCSIDVVYCTVVFMHLESWDRYAYVEEAFRVLRPGGKLYVDNVDLCT
                     DGGWAIFETHRRFPLAQRPDHITVCSTPQELREYLKRAGFEAIESHVGTELISVWGRK
                     PAPP"
     gene            17181..17891
                     /locus_tag="VNW28_06070"
     CDS             17181..17891
                     /locus_tag="VNW28_06070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXA09529.1"
                     /translation="MTKLTETDLASEFAKLAPWIFQFRIGDVDYGGTIGSGSDARIDQ
                     FFHFAPDVATILETGSLEGAQTFLLSRQPGVRRVLAIEGREANLRKARFVQELLGVKN
                     VEFVQGNLEEVDLARYGKFDAVFCCGLLYHLPKPWEFLARLPAVAPILYLWTAYAGDK
                     EAIALPNGMRGKIHSEGGSAEPLSGLSPTATWLTLDCLRACLVTAGYQQVEVIHDDPN
                     HVNGCMVSIGARTSPALG"
     gene            17933..18724
                     /locus_tag="VNW28_06075"
     CDS             17933..18724
                     /locus_tag="VNW28_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09530.1"
                     /translation="MKWFLSLTEDSTAVREYAEMLKVAVYTARKCTSLMPHLIYDGRE
                     NDFTIWLRRYDVQIIPHRSRFRDALGELGREKGNPHFEAALAGAFSRVELPEIVERLG
                     GAERVLYTDCDVIFMGEVVPELEANPCEYFAVAPEGVPSDYVNMNTGVMLMNIPRLRA
                     GLPEFLEYISQNLAELEKESWDEAAYRWFYRDANGPLWDKLRPELNWKPYWGENPAAK
                     IIHLHGPKPYQRDHIESTWPELRPYTGGAYHAVMDRWSELLAECR"
     gene            complement(18740..19774)
                     /locus_tag="VNW28_06080"
     CDS             complement(18740..19774)
                     /locus_tag="VNW28_06080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXA09531.1"
                     /translation="MTDRILQTSALGVTFAGNLDAPGPDESVYDQAIFLSGWICVPER
                     DPATWWVRAFLDDSCVAETRNFFLRSDVTRHLGVSASIPTGFRMLGKTPAAVTEAREA
                     TLRAVASCDGGRAQTFADRRVRLVPARLHEQPHGDVVHPDNATLLHRENIYGSGPPVE
                     NPPDEPANLIEAYLPAGASVVDVGCGAGAYGPRLIAAGHDWLGLEINPHCCEILRRRQ
                     MPFREVDRKSRRLPGRDREWECAICIEVLEHIEDPRLFVQEIARVIRGRALFSVPNLE
                     ILPYLHGWGVVPWHLLEADHKNFFTRTSLRHLLEERFGRVEVFPYGEHPLRSRDGIPL
                     YVHLFAVADP"
     gene            20093..22054
                     /locus_tag="VNW28_06085"
     CDS             20093..22054
                     /locus_tag="VNW28_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09532.1"
                     /translation="MRPEIAPLIRFDARHRALVLVVVGLFVGCVMLRLHGFSLPQWHA
                     QLDGSPPNEILLGQAREIRADDWAASLCTILAQGEHRPSFSVFNENIGLGSNMVVSPI
                     KTPVRHYITLFRPHLWGYFLGRDLGLAWHWWFLLLGLFYSYFLCFLLVSGGRFLVALA
                     GGLLVAYSPFFQFKSLEYAEIAIFAALVFVALIQVLFAPSRWERVAAALLLAWSVSGF
                     ALNFYPPSQISLGYLLGFLSGGFLLRHRAALRAQGQWGHRLICLVGSLGLAALAVGIF
                     VMQGWAILQILNQTIFPGGRFVTGGDYSWWRAFANNFLLQFFLTDNLPGTNICEYGSF
                     FFFFPVLAGWMAYRMWREWQADYLVVSMLLFCFALMTFAVVGFPAWLSAVTLMSKVPP
                     NRSMVALGIANAVLLCAIVASPQSRKPADAAVLVIAGLWGVFLFAVARAIAENYPSLS
                     LGLLILPAVAFTILGYGLLQERWKVTSILVLAALSIGGTCWFNPLVRGGSAYLYRNPL
                     AAKMRALSLRDRRARWATFGQMFYADYPRLLGLPALNGTHPYPQFDLWARFDPERRWV
                     DQYNRYAHVWFATAAGGITIESPFLDQIKVSLSPANPVLESLGVKFFLVVSPEDRFFE
                     RRPQQFSKVFSQGRIHIYRRRGEEKSRAP"
     gene            complement(22124..>23204)
                     /locus_tag="VNW28_06090"
     CDS             complement(22124..>23204)
                     /locus_tag="VNW28_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXA09533.1"
                     /translation="HLDPKGDRIEDADLAFTGWVAADRPVQAVWLPAAGKQRLTTCAR
                     PDVRRVFPNRTALGFSGKCKGSEIGPDGLRLAIQLGDETFEIEHPLPPPLPRPSLPQR
                     IAAALQSAWLRGRARLTNDPSRRWSLTLRRHLLSRRLRSGVFRRSHTDALLADFATAV
                     PDAIFLQIGANDGFTGDPLNHLINRPDTRWRGVLVEPVAHLFAKLSERYGHDPALRLE
                     RAAIGERDGTIEIHRLQTTAEDSLWLQQIPSLDRALLQRNAREFGQAERPTVTEVVPC
                     LSVATLLARYQMDHLDLLVIDAEGWDWRLLRQFDLARLEPKLILYEHHHLAPAERAQA
                     HQFLAQHSSDWAETEEGDTVAWRLD"
BASE COUNT         4705 a         6773 c         6983 g         4743 t
ORIGIN      
        1 gccagggctc caaagactga tgttatcatc gataacatca gattgtcccc ggaaacattc
       61 gggattgcgg ccggtgtgac agtgatgtgg accgctgata gtcgggtcca gcaatcacct
      121 gtcttgaaaa cggggcagag cttttccgac accgtcgcga cagcaaaaaa ttactcttac
      181 ctctgcgcga ttcagcctcg cgtgacggag aagacgatcg tcagataacc atcaacccca
      241 acccaactct atgaaatcag cactctgctt aactcgcttg aaaaccgccc tcttagttgt
      301 tccactgagc ttgtactttt gcgggccgag aatggcagaa gggagagata gaagtctgtt
      361 gcctcctggc gtcgtaaggc caggaaaccg ggattatctc gcccgatcga ccgaagggtc
      421 tctaacggtc tacgtcacgg gcgatgaatt caatgatgcg tggtattttc cgcgcagcgt
      481 ttacgcgatc tacacaatcg acggaaagct ctttaaaagc gtcgaaagcc aatattccgc
      541 ggacgaagaa attccggatg tggtggcgct gccgtttgga tcttacctgg tcattgctcg
      601 atcggagaag aaaggatata tccgccggcc cgttgttatc gaggcgggcc ggcgaacgat
      661 tctcgacttg gatctcagag aagagggctc actgaggcgg atagcacaca gctgacggtt
      721 cgagaatccg cgcaaagttt cggagaggac cgtatgaatc cgtttgacct gaaggccgcc
      781 ctgcttgcga aacacgcgca acacccggta atcattcatt ttccgatcgc gctgttcatt
      841 atcagtgtgg cgtttgatct cctcgcgatc tggagaagga atccggcggt ggcgaaggcc
      901 gcctactata atcttctcgg ggcggcgctg acggcgccgg tcgccattgc cagcgggttg
      961 acggcatggc agtggcaatt ggaaggtgcg aaactcaagg gaaacttgct cttgcatctg
     1021 atcttcgcgc tctcggcgag cggaatgatt tggctgcttt gcgggtggcg tatttcccag
     1081 cggaaacgtc cccaaccaac gccgggactc atctatatct tgcttgcact tgctacggtg
     1141 ttgttgatcg cactcgcggg acatttgggg ggattcgtga gcggcgttga gggcggccca
     1201 acctgagttg tgaagtcctg cgctgttttc tatgtcgaaa gaccgcacga tctccgaagc
     1261 gagcaacgag tttgagacga agtacgacaa tcgcttccgg aaaaatccgc gaagcagccg
     1321 tcgcggcgat aagagaatta agtgtttttc gttcggcagg atctgtcacg caaaaaacac
     1381 ttgatgcaga agtcgcgcgc attgcgcaag agaagttgca gccatgcggg tgcgatctcg
     1441 ttgtttgccg aatggatgac ggcgtcatgc ggttcttaat aaagctccgg agcaccggga
     1501 gagaatacga tctaatcaag agttttttcc atcgtgacgg tgaatcgatt cctcaaacgg
     1561 aagtatgact cttgaactgg acggtcaaaa ggtagttgcg atcggtggaa aacgggaggt
     1621 cgatcgcacg attgttgaag ccggaaggag ctgacggggt gatctggatg cgcgtgtgga
     1681 aaggtgacgc gagcgaatcg aaacagctgc tgctcaaaat ggacgcacag ggccgtaatg
     1741 aagcagccca acggaagatc gtcacatacc tcactctggc gcgaggtgat gcgggcacaa
     1801 ggcctccgtt tctggtcgca tcgctaccgt cgaagatttt cggaataaaa tcgcgtggca
     1861 tcggtaagga caagtgtagg caatccgcca acacctaact aaaaccaaat tcaaacccac
     1921 attctatgca ttctgatacc aatcattccg acgaagacaa cacctcaaca gcagcagaat
     1981 catcccgacg tgcgatctcg gttccggcgc cgaatcgcct cgccagacga gcctttctgc
     2041 gcaatctcgg tatgggagcc gctctacttg cccccggagc taccctgcta ggcaatgctg
     2101 gcaaggcatt ggcagctggc aatacaaagg aggacaatgc tctctcacga ggcgatgttg
     2161 cgatcctcca gcttctcgcc gccgccgaac tgatcgaagc cgatctttgg cagcaatacg
     2221 ccgaactcgg cggggttgat tcgccaacga caggctacac ggctggcctt caaattctcg
     2281 acggggatca gctccagtat atttccgaca acacggatga cgagcagagc catgcggctt
     2341 tcctaaacgc ctatttggca tccaaaggcg agtcgcaggt cgacttgagc cagttcaaga
     2401 atttggctcc cagtaaagtt tcattcgttc cgcaaaccgg acggctgacg aacctgaaag
     2461 agctcaccgt agataccagt tggtggaccc gttaccgcag cacgaccaac cctgatttcg
     2521 gggcgacctt cccggatgcg gtcccgagtc taaataccgg tctgcatacg gccattcctc
     2581 gagttgatgc cgaactggac gatccgaaca acccgagcga tcacatcaaa gcgattgctt
     2641 tcacggcggg atttcacttt ggttttattg agcaaggtgg caccagcctc tacgcgacgc
     2701 tggctcagaa ggtaaccagc ctggaagttc tacgaattct cctcagtatc ggcggatcgg
     2761 agatcatgca ctttcagacc tggcaggaca aggcaggcaa tgcgactcct ttgaccgact
     2821 tcgaccggat caacaattca acggtgacct ttgttgacct caccacgggt cagccggaga
     2881 cgctgcaagc caacctgatc atgcccgagc cgtgcgagtt catcagtccc aatctgcctg
     2941 cctgcgcaat tattcgtcca acgggcccag gacagctgga cgcaaccgga gctattaaca
     3001 gcttcatttc cgacggtctc tttcgcggac aaccccaaac ctttttaaag ctattgagct
     3061 ctctggcgcg ggcagcggat gcggcgcacc gtgagatcgg tgattgattg cactccggca
     3121 gttcgaatgc cgcgctgggt ccgctcagcg cggcattttt gtaaatacgc ggacttccgt
     3181 cactcaatta tccaagattt cgggaataaa atcgcacggc gccagtaagg acagatgtaa
     3241 gacaatttct ctaagcaacc aaacgaccta aacagaacca gatattatgt ccgattacat
     3301 ttctcacgac cacgaacacg acgggattga tcgccgcggt tttctacaat gcatggcttg
     3361 ggccggtacc ggcctgcttt ggacgatcag tggcggcgtc ctcgcatcga aaaccctggc
     3421 ccagatcgcg cagagcggag attcgctctc gaaagcgacc gatctgagct tcttacagat
     3481 cagcgacagt catatcgggt tcagcaaaga ggcgaacaaa gatgtgaccg cgacctttcg
     3541 aattgcgctc gatcgcatca acgcgatgcc gacgccaccg tctttcctta ttcacacggg
     3601 cgacatttcc cagctatcga agccggaaga gttcgatacc ttcgatgagg tgctgaagag
     3661 ctgccggacg aaggatgttt tttacgttcc gggagagcat gatgtgctcg acgggagcgg
     3721 aaaactctac cgtgagcgct atttgaagag cgtcgaaggc cggggctctg gctggtacag
     3781 cttcaacaaa aacggcgtcc atttcattgg cctggtcaac gtcttgaatc tccaggccgg
     3841 cggcctggga caactcggag acgagcaact ggaatggctc gaggccgacg tcaagggtct
     3901 tgcatcgagc actccgattg tggttttcgc gcacatccct ctctgggcgg tttatccgga
     3961 gtggggctgg ggcacgagcg acagtgaacg cgccttggga tatctgaagc gcttcggctc
     4021 ggtgactgtg ctgaacggtc acatccacca ggtgctccag aaggtcgaag gtaacgtgac
     4081 tttccacagc gcgatgtcca ccgccttccc gcagcccgcg cccggaacgg cggcatcgcc
     4141 cggaccgatg aaagtgccgg ccgaaaaact gcgcagcatg ctcggcctaa caagcgtcaa
     4201 gtatctgcag ggcaagtcct ctctcgccat cgtcgactcc aacctcagtt aagagatcaa
     4261 cacctatgta tacatccgtc actcgaaacc agaccgtccg aatcctgttt accatcagct
     4321 ccctatgttt gagcagcctt agtttcggcc aggccgagaa aggggcgtca gcagacaata
     4381 ccaaaacgaa ccaaatagtg attaaggatt tccacttcga tcctcaagtc ctcaccgtaa
     4441 ggtccgggga aaagatcacc tggaccaacc gggacgagga accgcacacg gttgtgagcg
     4501 tcgagaagca gtttaagaaa tcgggcccgc tggacacgga tcaagaattc accatcacag
     4561 cgggcgcccc cggcacttat acctattatt gctccgttca cccgaagatg actggtacga
     4621 tcgtggtcaa gaaatcataa tcgaccgcca gcgcctcatt tcgagagcct aggtctgttg
     4681 acaacgacta atctatctca gacgaagccc taaaacaatg aaaccgacac cagatatttg
     4741 caatcgacgc aaatcgggca cgctggccgt gtccaccagc cgggtcttcc cgaagaccga
     4801 ctttaattac caagcaacga cactcgccaa tctcgagggc cgatgggccg atctctgcaa
     4861 gtcctccttc cgcgcgatca gccaggatta cttcgcaaac gaggcgcctc ggcacttcgc
     4921 ccacgaggca gggttgtttt tcatgatggt gatgacggtc actttgccgt tattaaatgc
     4981 gtcggccgcc gccctggggc tgatccggat tcgataacct ttcagccgtg catagcgggg
     5041 gcggggtcac gtagatatac attttccgcc ttcgtggatt cagttcggaa gcgttcgact
     5101 cgggcgaaca tgcgatgtgg aaggaagagg ccaacactga cttgggcgcc gtgacctccg
     5161 acccaatccg actaaattcg acgatgaaag caactcgaac tggaaaaatc gcccgcttga
     5221 cacgtccaat ccgacaagaa ctgaatcgtc ggctcgatga aggcgactag gaaagaaaat
     5281 cgtcgcctgg ctcaagagcc ttccagaggt gcaatgcatc gtggtggcag agttcaacgc
     5341 taaaccgatt cgggaacaga atctctccga atggaaacgg ggcggctatc gcgactggga
     5401 ggcgcagcag gaggcgatgg gaatcgccga gtgcctcggc gcggatgcgg cagaatggac
     5461 cggcgcggat cgcgcttcgc tcaccgacac gttggcgcac tttctggtcg cccgctatgc
     5521 cgtggcgacc cggcgtgtgg ctgaaaccgg agggcgggaa ggatggcatc tgctgcgtga
     5581 aatgggcagc gccatcgtgg agctgcggaa gggcgatcac agcgctaagc ggctgcaaat
     5641 cgatcgcgag cggttaaagc tggaaacaga gaagtcggga gaaacagatc cgcgaaaagg
     5701 tcgcagcgct gttgaaacaa cagggggcga agggagggct caccccggag gagcgagcgc
     5761 ggcgaatgcg cgaaatcttc gggctggcac tcgaagaaaa aggaaacggc gggatttcct
     5821 ccgaaacgct cgcggagatt gaaccggctg cgaaaccttc ttgaaccggg aatttcgaat
     5881 gacgcgaagg cagccatcca atcagattaa ggagtgcgct tcatgcactt ccatcccggc
     5941 ctcacgcatc cagccgccga cgcgattgtt cgcgcggtgc atcgccggcg gcagaaatgt
     6001 cctcgttagg cggccgggcg gccgttcctg gtgtccagaa tgtgaatcgt taggctcatg
     6061 tcaccagatc acgcaaacgc agttcggcga tctggcagat ctcggccagc gccgtcttga
     6121 tctcttccgc gcgcgaattc tgcagccgcc ccggcagggc cgtcagaatc gcctcccttt
     6181 tgttgcgccg cgcgcagatg acgaaaggga atccgaattt ttcgcgatag cgccggttga
     6241 aatcagtgaa gcgttcgatc tcctccggcg agagctgatc gaggccggcg ctcttcaatt
     6301 cggcttccga ttcgtgggtc aggaccgcgc gattgacgag gtccggatgc gcgcgaatga
     6361 gcgcgagttt ctcgtcgtcg ctcgcctggt tgacggtgcg acaaagcgcg gcgtgcaatg
     6421 cttcgcgcgt ggcgaacggc cgcgccgccg cggtccgcgc cgcgacccag ggagaatgct
     6481 cgaagaccgg acgaatgcgg acggtaaaca tctcggggtc gaaggcattg acttcgtcga
     6541 gcgtcagcat cgcgtttccc gtaagtgctt tcgtcacagt tcgcaatggg gaaatctctc
     6601 gcgttctcgg ggtgaaatcg cggtccgcgc gaccgtggga atcccatccg ttccaggtaa
     6661 aaccaatggt cgaaaatcgg gggatgtcgg ccggggaggc ggactacggc cagatgacga
     6721 gaagatgaca actttaagat gttgaaaact agatggttaa actataacgg agcgcttgga
     6781 gttggcggat cgaaaattgg tgctcgacag ccttgaaata atgaacaaga accaatctga
     6841 gactgtctca attccgatat ttaaccttgt taagtagttc tttaaatgct acaacacgca
     6901 tcccgtaact gcctggcgtt cgtaacagct ggcagctcgt cgtttcgaca ccccactttc
     6961 gatccaattc atgagaactt atatggccgc tgaagatagc gatactggta taaagattta
     7021 cctgcgcgag atcgggcaga tcccgctcct cacgccgttg caggaaatcg agctcgccgc
     7081 gaaaatcaag aaaggcgaca aagaagcgcg cgctttgatg attcgctcca acctgcgtct
     7141 ggtcgtcaag atcgcgcacg actacgccaa cctcggtctc ccgctactcg acctcgtgtc
     7201 ggaaggaaac attggcttga tgaaagccgt tgagcgtttc gatccggcga agggcggcaa
     7261 gctcagcacc tacgccgcgt ggtggatcaa gcaatcgatc aagcgcgcgc tggcgaacca
     7321 gtcgaagacg attcggttgc cggtgcacct cgtcgataag atttcgaaga tgcgccgcgt
     7381 ctctttgcag atgagcgaag agcttggccg cgaaccaacc gacgacgagc tcggcgaaga
     7441 aatcgggatt gcggcgggca aagtctcgca gctgaaaacc gtttcgattc gtccagcttc
     7501 gctcgacgcg ccgatcagcg acgatgactc gactgaattc ggcgagatcg tcggcgacga
     7561 agaagcgcag acaccgtttg aacttttgcg cgacaagaat ctgcgcaacg aggtcggcgg
     7621 tttgcttgac gtgctggatg atcgcgaacg caaaatcatt ttttcgcgct tcgggcttga
     7681 cggcgggaag ccgaaaacgc tcgaggaagt cggcaagaaa ttcggtgtga cgcgggagcg
     7741 catccggcag ttacagaaca tcgcgctttc gaaattgcgc cgtgcgctga gcaagaaaga
     7801 gcgtccggtc gacgtcgaac tgacggctga agtctaaccg gcaggcagaa gaattagcag
     7861 agcgcgcgga ggcaaactcc gcgcgttttt tttgtcgcgg gctcaggcct tgcgcagctg
     7921 cacgcaatcc accatcgcgt ccccgctctg cgtgtcgctg atgatgacaa gattgtcccc
     7981 atcccgcgag aggcgctgga cgcgcagata attctccgcc gccgtgatgt tgccgttggg
     8041 gtcggcggta aaatcgaggc gcaccggggc cgttccccag caaagagcga gttgccgata
     8101 aactgattcg ctcggggtaa aagcgaaaat cggcgcatgc tccggccgca tgttggcaac
     8161 gtagcgcgcc atcgtcccgt ggcgggtgaa gacgatgagc ttcgcgttcg gcaaagagtt
     8221 ggccagcacc acagcggacg cgacggtctt ttgccgcagg ttttcgagaa tcgcatcccg
     8281 cgagaagccg gcgccgccgc tgcgctcgat tctgcgcgcc acccgatcca ggaccttgac
     8341 gcattcgacc ggataacggc ccatgctggt ttcaccgctc aacatgatgc catccgcctg
     8401 ctcgaacacg gcgttcgcga catcggtgat ctcggcccgc gtcggaacgg gattgacgat
     8461 catcgattcc aacatgtggg tcgcgacgat gaccggtttg ccgatccgca aggagcgctt
     8521 cacgatccgt cgttgaataa ttggcagctc ttccatcggg cactcgatgc cgagatcgcc
     8581 ccgcgctacc atcgtggcat cggctgtctt gatcatttca tcgatctgtt tcacggccga
     8641 ttgctcttcg atcttggcca cgattcgtgc cgtggacttg ttttctttct ccgccaaaac
     8701 cttgcgcaac gcttcaaggt cgctcgtttg tcgggcaaaa ctgagcgcca caaaatcgac
     8761 cgccagctcg accccgaggt ccacatcgcg caggtcctta ttggtcagcg gcggcaggtt
     8821 cacatgcacg cccggcaggt taatgtggcg gcgtgaaccg agggtgcccg cggtcagcgc
     8881 tttgcagaga atgcgattct tgcctttctt catgacgacc agctggagca ggccgttatc
     8941 gatcaggatt ttgttgccta acgaaacgtc gtcaattaag ccatcgtaat tcacgtcgac
     9001 cgaatacgcg gcttcgcctt tcgcgccgcg cacggttaat tcgagaagat caccggggtt
     9061 caattcgatc gcgtgggcca atgctccggt gcgaatggca gggccttgcg tgtcgagaag
     9121 gatggcgagc ggatgttcga gatcgttcgc gaattttcgc gcgcggggca caatgtcgcg
     9181 gacccaggcg tgctcagcgt ggctcatatt aatccggaac acgtccgcgc ccgccagcat
     9241 caactcctgc agcatctcat ttttttccgt cgccggaccg agcgtgcaga tgatttttgt
     9301 tttgcgcatc ccgcaaatca ggattccgaa tgcccgctga aaagcaatca gtattaagcc
     9361 gcgacctcag gagcgcggcg gaaacgcgat ttcatagccc ggcagggcga gcgagaggtt
     9421 gggattatag aaagggtcgt gcaacaacgc ggcgccgtgg gtttcctgca tgtaggtcgc
     9481 ttcccggaaa aactgggcct gctgcttcgg gtcctcatga tggccgcgcg agccggattc
     9541 gtcgtggacc agattcgcgt agggcgtcca gaccacttgc cagccccgcg cgcgcacccg
     9601 caggcagtag tcgatgtcgt tgaaattgat cccgagatct ggctcgttaa atcccttcag
     9661 ctcctggaaa acggctttcc gggtcagcat gcaggccgcg gtcacggcgg agcagttttg
     9721 ttggagaatc gcgcgattga aataacccgg gtgaccagcc ggcacgcgat ggtgcgcatg
     9781 cccggccacg ccgccgagcc cgagcacgac ggcgccgtgt tgcagcgttc cgtcgcgata
     9841 ccagagacgc gcgccgaccg cgccgacctc tgcgcgcgtg gcgtggctca ccatctcagt
     9901 cagccagccc gcttcgctca cttcgatatc attgttgagg aacgcgagaa tctcgctgcg
     9961 agcctggcgg gccgcccggt tgttcaggcg actgaaatta aacggacccg gctcgcgcaa
    10021 aatactggcc gcgccttcgg cttcgagttg gcgcaggtaa tcgagcgtct cgttctcggt
    10081 ggaatcattg tccacaatca ggatatcgaa cgccggataa tcagtcacgg cacgcaagct
    10141 ttcgacacag cggcgcagca acgcgatccg gtcccgcgtc gggataatga tcgtgacccg
    10201 cggagccgga ccggggagcg cgtagatcac gcggtgagat tcattgttct ccggacaagg
    10261 ttccacgcgg cccgcaatcc ctttgcgagt caagtgatcg cgcagcgtgc gccgggccgc
    10321 ttcacgcgcg tagggttttg cattcggcaa cgccgccagg cttccgggga ccgagcgcca
    10381 gtgataaaga atgcgcggaa tgtgccggac ctggtctgct cgaatgcgat caaaacagcg
    10441 cgccgcgaga tcgtaatcct gcgaaccctc gtaaccttcg cggaagcctc cgatctcgcg
    10501 caggagcgct gcctcgtaaa cgcccagatg attgatgaag ttctggccgt ggaataattc
    10561 cggattccaa tcgggtttga agaacggatt cgagcgcacc cccgccagat cgatcttgtc
    10621 ttcgtcggaa taaatcacgc gcgcttcggg atgcgcgtcc atctcgagtg ccaccaaggc
    10681 gagcgcgtgc ggggcgaatt cgtcgtcctg atcgagcagc gcgcaccact cgccggtcgt
    10741 gagggcgagg gcagaatttg aggctgcgga gatgtggccg ttggtctcgc ggaaggtcag
    10801 cttgatccgt ggatcggagg cggcgctcgc ttcgagaaat ggccgcactt tgggatcggt
    10861 cgaagcatca tcggcgagac agagttccca tttttcgtaa agctgggctt tcacggaatc
    10921 gattgccgtc gcgaggactt tcagttccgg gttgtaaatc ggcagcagga cggaaatccg
    10981 tggccagcgg cgcaatgcac cgagttgctg gcgcagcgcg atccgctcgc cggcgcccgg
    11041 agtgtcgtag ctcgccaccc attgtcgata agtgtgcagg tctcttttcc gaatcgtgcg
    11101 atggctcttg ctcgcgattt cgatcgcctc ctgaaaacgt ttgccgaaac gatcgagcgc
    11161 gaagtgccgg acaaaggttc ggcgggcgga tgctccgcaa aatttccgca gctcggggtc
    11221 ggccgcacat ttggtgattg cccgcgccag ttcgttaggc ttttccgccg gaaccagcca
    11281 gccgtttaat ccatcccgca gccattccgc gatgccgcca acgttggcgc tgatcacccc
    11341 cttgccgagg cccatcgctt cgatgatcga aatcggcatc gtctcgtcgc gcgaggaaca
    11401 gacgaggaca tcgatctgcc cgaggaattg cagcgaagcc gggtgctcga gcgcgggtcc
    11461 cagatggacg ttttccagcg gcgcggcgcg cgactgcagg tcggcgaaaa agtcttcatc
    11521 gaggatgcgt cccgcaaaac cgaaagacgt ccgtcgccgc aattccggtt caagtcgcgc
    11581 gatggcgtcg agcagaatgt cctgtccttt gcgcggctcg tagctgccga gacagaaaaa
    11641 tttcagcgtc tcctcgttag gcatttttct cgccaccgct tcgatttctg gaattccgta
    11701 gggcacgacc tcgatcctgg aacgtgttgc gccttcatag aggcgggcgg tttgccgcgt
    11761 tggagtaatc agcagatcgg cgagattgag ggttggctgg atttcggaaa ttttttgcat
    11821 cagctcgacc gcaaggagcg tttcgtgcag ataccagatc gcgcaccggc ccgcggcccg
    11881 cgccgcgcgg atcgcctgcc aactggccag cgtgtttgcc accacggttt cgaaatccgc
    11941 gcagagcgcg tcgagttttt cgcgtttcgt atccgtcagg aaagtcgcgt cgaggatcgt
    12001 ctcgatgccg cgcgccacga ggagatcgga aatcgggccg cgctccggcg ccgccaccac
    12061 tggcggcgtc ccgtgttcgt ttaaccagca ggcgagataa tacaaggcga gcggtgctcc
    12121 gctcagtgtc gtctcgtgcg aaaaaagcaa gatgggacgg gtggctccgg cggctcgcga
    12181 cataagattc ttagagcgaa tggacttccg ctgcggcgtt cgaatgactc acctcgttca
    12241 tgagctcgac gaatccttcg gcgaagcgat cgaacgtaaa atatttttcg taggtcggcc
    12301 gcgacttcgc gcgcagccga tcgatcagtt ccggctcccg caccagccgt tggatcgcct
    12361 ccgccaaggc ggttgcgttc cccggcggaa aaaataatcc ggcttcctcg ccgatcagat
    12421 tttccgccac cgcgcccacg ctcgtgctga ggatcggggt gccgagcgcg agtgcttcga
    12481 tcaatatgag cgggccggtt tcatcccatg aagccgagac catcacatcg gcagcgcgga
    12541 tgagcgcgag tgcctgttcg tgagtgacgc tttcctcgta ggaaaggtaa gagtgctcgc
    12601 gcgccgcggc tttgatttcg gcggtcagct cgaggtcatg gggtcggccg acgatttgga
    12661 aatggacgtg ttgcaggact tcggtgggga gctggcgcag cgcattgaga aggacctcct
    12721 gccccttccg ctgctcgatc gtcccgagca cgaggaatct cacctgttcg atctcgcgct
    12781 cggcggtgct ttcaagcccg gcgcccggat caggaatgcc gtaattcaga acgcgaatcg
    12841 cccggttcgt aaacggctga tagacctgag agctgtgcgt gtccggcgtg atgatcagat
    12901 ccgccaggcc gagggcggca cgcatgcggg cgtcgctccc gaggtaatgt tttgccactc
    12961 gtccctcatg aatccaccag acatgaggaa ttccagcggc cttcgccgcg cacacgatcg
    13021 gcgcgccgaa gatcgtgctc gcgatcacgc aatcgaattc ccgcgcgaat cgcgcgaacg
    13081 acggatgtcc ctccatcacg agcggatcga cgatcaacgg aatcccagcg tcgaagaatt
    13141 tctgccggac cggaccttct tccggcgaca tgacggtgac gaaaaagccg tgctctttgc
    13201 accattgcgc gagttgcaac agaatcagcg gcgcgccgct ccaggtcaga tcgtgcgaga
    13261 cgaataagag gtcgcccttc ttcgcgggcg cgctcgcctg gtcgcgcgcc cacatccgaa
    13321 tcggcgtcgg cgaatcggcg taaagccaat cgcgcacgtt atccggaaaa tagggatcgt
    13381 gacaggtgta ggccggccag cgctggagga gaaagaccga cgccttctcc cggcgcggga
    13441 tcgttgccgt gtgttccacc gcgccgatgg aggcgtgacc gcgatgccgc atcgtgatga
    13501 acggcgtgta aacgcagcgc agaccggcgg cgcgaatctt gaaacaaagg tccaggtccg
    13561 agtgcgcgat cggcgtgttg accgcatcga attcgccgac cgaaaaaaag tcttcgcttc
    13621 gcatcgcgag gcacgcggcc gacagggcgg acacgtcgcg catcgattgc gcgaaattcg
    13681 tgtagtcagg cgaatcggcg ggccattggt gacaagcggt cccgaggagc ccgcgcacgc
    13741 ccgtgaccag gccggcgtgt tgtatcttcc cgctttcgta aagtaatttt ggcgcgactg
    13801 cgccgaccgc cggattttcc agcggctcga tcaggttttg aatccaatcg ggctggccgc
    13861 tctcgacgtc gtcgttcacg aaaataagac gttcgccggc ggcggctcgc gccccgagat
    13921 tgcatttgtc ggaaaaattg aaggcctgat cgtagcggac gaagcgaaac gcgggcttct
    13981 tcggcgcgat gatttccagt tgttcggcca gtgcgctgtt agtgacaatc accacctcgt
    14041 agtccgggta ggaggtcatc ttcggcaatt cctcgacgca aaactggccg cgcagcgcgg
    14101 agtccgtcgg gatgacaatc gaaactttcg gccactgcga aatcctgagg cgcactcgat
    14161 tggcggcggg atactccaag atctcggccg ccagtccgcg gcgttccatc gcggcggcga
    14221 gcgcggctag attcgttttg cgggcctccg gttttccgcc ggccgatccc gacgcgggat
    14281 gttcccgcca atggtagagg acgtgcggca cgtgacagat ttcgcgggcc tgttcggtcg
    14341 cgcgcaaagc gaaatcataa tcctgcgaaa gatcgaactc gcgccgaaat tggccgacgc
    14401 gcgcaacgag gtcccgccga tatagcgtga gatggccgag atacatcgag gagagcagat
    14461 attccgggct ccattccggc ttgaaaaacg gggagtggcg tttgccgtcc gcgctcaggc
    14521 gatcttcgtc gctgtagaag atctcgccgc cagggtggcg cgcgatggcg cgggccagtt
    14581 cgtaaagcgc gaagggcggg agccggtcgt cgtgatcgat gcaggtgata aaatcgccgg
    14641 tcgcgagcgc gagcgcgcgg ttcgtatttt cggcaatgcc gagattctcg ggcaggaact
    14701 cgacgcgtag ccgcggttcc tttttctgcc aggtcgttag gcactcgatc gtggcggcag
    14761 cggaagaacc gccgtccacc gcgcagattt cgaaattgcc gtagctctgg gcgccgagcg
    14821 acgcgagtag ctcgtcgaga aaagcggtcg gcgtgttgtg agttggcagg agaaaactga
    14881 tcttgggttg aaccgggagc gccgtcgcat ccgcgtgttg cgcctcgagt gcggctgcat
    14941 ccggctcatg ctgctctatc catttcgcgt agcgagcgat cgcggtctcg ggcgccggtg
    15001 atttctcgac caagggcacc tcttttcgtt ttgccaagcg gccgaggttt gccgggatgc
    15061 ggtgtaattc gcgcaggacc cgctgcggaa tcgagcggcg ctccttcttc tttccgtcgg
    15121 aggaggcctg aaagcagcgg ccctttttct tgcatgcttt tttggccgcg ttcaggatcc
    15181 gtttttgttc ggcgacggtc gtcttgagct ttttgttttc ggcgctcagc gcctcgctcc
    15241 gaccgaccca ttgtttcaac tgctcggcct gcgcgtccat gatgagctgt gccttgatcc
    15301 gatcgcgatg caggtcctga aacgcgcgct cgtgctttcc ggcatcaatg accccccgca
    15361 gatgagcgag gtcgcgcgcc agttggtcca tcacttcctg cgccttccaa cgatcttgca
    15421 aaagcgtatc gagccaagcc tggcgcaatt gcaaggccga cttctggcgc tcggcttcgc
    15481 tcacgcgatc ttgcgcttca atccgggccg cgatcgcctg atagagctgc ttcaagtcgc
    15541 gccgcccttc ggcgggtccg aaaagagccg cgcacaagct gtcgtccgct gaaacccccg
    15601 cgctgaggct ggccacaccc agaccgattc cctcgtcgaa ttcgtagttg ggccggccgg
    15661 cacaatattc ctcccaggct gcgcgcggcg ggctttcgcg ttcgagcagg gtgccgtgca
    15721 ggagaaccag cgcctgcggt gcgagcttcg gccgccacac gcgcagttcg gcgcgcagac
    15781 tctcggcgga atcgcaatcg acgatcagca ggagatcgac agtgccatct tcatgttgcg
    15841 cgccttcttc gcgtgggtct cgctcgcgca gcgtccccca gtcatgaaaa aactcctccg
    15901 acttttcgat cgcaccctgc cacgcgacgt catcctccgc gttttctccg atgcgcggtc
    15961 ggcggatcgt taggcagtga gaggtgagtt tttgctcgcg cgccgcctgg caaaaagtga
    16021 aatgcacctg ctcgtcctcg aaaccgagcg tgaccacctg acgcggccgc cgcagggcga
    16081 ccaggtcata aaggaacggc agatgaaaac gcgcgacgcc gctggtgaag aacctgggtt
    16141 gaaatccaag gctgggaagt ggtggcagca tcgcgaatca gcccagtctc acgccgggca
    16201 cgaattagtc gtcgccgccg gcgcgtgaat caagctttcg ccctgacttt ccgccgcgtc
    16261 acgcctggag cacttttacg cactcggccg gggagcggtc cgggcggtgt cgagttgaat
    16321 cccggcgttg ttcaccaggc cggtgtcgcc ggctgagacg ttgtcgatca gctcatcgtc
    16381 attcacggtg gcgccggttt ccttccccac acggagatga gttccgtccc gacatggctc
    16441 tcgatggctt cgaacccggc ccgcttgaga tattctcgca actcctgcgg cgtcgagcaa
    16501 accgtgatgt gatcggggcg ctgcgcaagc gggaagcgcc ggtgggtctc gaagatcgcc
    16561 cagccgccgt cggtgcaaag gtcgacgtta tcgacataga gtttgccgcc gggacgcaag
    16621 acgcggaacg cttcctccac atatgcgtag cgatcccagc tttcgagatg catgaagacg
    16681 accgtgcaat agacgacgtc gatcgagcaa tccgcaatcg gtcgcaggtc atagcccgag
    16741 agttcgacaa gctccacatt ccgaaactcg cgcagccgtt cggccgcgaa accgagcatg
    16801 tggggcgaaa cgtcggcccc gatccagcgc cggcaacgcc ccgccaggca ttgcccgacc
    16861 cggccaatcc cgcagccaat ttccagtacc acatcggtcg ggctgatgcc gacattcttg
    16921 aagagaaaat cgctcgtgcc gacgccgctc gtcgcgattg tggcctcatc ctccgtcccg
    16981 ctgacgtgca tcttggcgct ggagagcgtt tgcgagagcg ccgtccacgt acccttgtaa
    17041 tctgaacgca tggctaggcc gcgctgcccc tctcacggtg gaaggcggca gcaggtgata
    17101 gtatggtctc cggtgcgccg ccgcaaccac agccaaaatt tccgcaaaac cgtggcctct
    17161 tatttgcggg agaaaagcgg atgaccaagc tgacggaaac agatctggcc tcggaattcg
    17221 ccaagcttgc gccgtggatt tttcagttcc ggattggcga tgtcgattac ggcgggacaa
    17281 tcggctccgg gagcgatgcc cgtattgacc agttttttca ctttgcgcct gacgtcgcca
    17341 ccattcttga aacgggctcg ctcgaaggcg cgcagacctt tcttctctcg cgtcagccgg
    17401 gggtgcggcg ggtgctcgcg atcgaagggc gcgaggcaaa cctgcggaaa gcgcgcttcg
    17461 tgcaggaact gctcggcgtg aagaatgtgg aatttgtgca gggcaatctg gaagaggttg
    17521 atcttgctcg ttacgggaag ttcgacgcgg ttttttgctg cggccttctt tatcacctgc
    17581 cgaagccatg ggaatttctt gcgcgcttgc cggcggttgc gccgattctt tacctctgga
    17641 cggcctatgc gggcgacaag gaagcgatcg cgttgcctaa cgggatgcgt ggcaagatcc
    17701 acagcgaggg aggttccgct gaaccgctga gcggactttc acccaccgcc acttggttga
    17761 cgctggattg tctgcgggcg tgcctggtga ctgcgggtta tcaacaggtc gaggtcatcc
    17821 acgatgatcc gaaccatgta aacggctgca tggtttcgat cggagccaga acgagtcctg
    17881 cccttggatg agggcggctc tgccagttag gcacgattag aatttccggt ctatgaagtg
    17941 gttcctttcg cttacggaag attcgaccgc cgtgcgcgaa tacgccgaga tgctgaaggt
    18001 cgcggtctac accgcacgaa aatgcacgtc gctcatgccg catctgattt acgatggtcg
    18061 cgagaacgat ttcaccatct ggctgcgacg atacgacgtg cagatcatcc ctcatcgcag
    18121 ccggtttcgc gacgccttgg gcgagctcgg ccgggaaaaa ggaaacccgc attttgaagc
    18181 ggcgctcgcc ggcgcttttt cgcgggtcga gttgccagag atcgtggaac gtctgggcgg
    18241 cgccgaacgt gtcctataca cggactgcga tgtcatcttc atgggtgagg tcgtgcccga
    18301 attggaggca aacccttgcg agtatttcgc ggtcgcgccc gaaggagtgc cgagcgatta
    18361 cgtcaacatg aacacgggcg tgatgctcat gaacatccct cgcctgcgag cgggcctgcc
    18421 ggaatttctc gaatacattt cgcaaaacct ggcggagctg gaaaaggaat cgtgggacga
    18481 agcggcctat cgctggttct accgcgacgc gaatggtccg ctctgggaca aactgcgtcc
    18541 cgaattaaac tggaagccct actggggcga aaacccggct gcgaagatca tccatctcca
    18601 cggaccgaaa ccctatcagc gcgaccacat cgaatcgacc tggccggagt tgaggcccta
    18661 caccggcggt gcgtatcacg cggtgatgga caggtggtcc gagctgctgg ccgagtgtcg
    18721 ttaggcgcgc gtcgcgtttt cagggatctg cgaccgcgaa aaggtggacg taaaggggga
    18781 tgccgtcgcg cgagcgcagc ggatgttcgc catatggaaa tacctcgacg cggccgaagc
    18841 gctcttcgag gaggtggcgc aagctggtgc gcgtaaaaaa attcttgtga tcggcttcca
    18901 gtaaatgcca gggcacgacc ccccatccgt gcagatacgg caggatctcg aggttcggca
    18961 cggaaaagag cgcgcggccc cggatcactc gcgcgatttc ctgcacaaag agtcgcggat
    19021 cttcaatgtg ctcgagcact tcgatgcaga tcgcgcattc ccattctcga tcgcgaccag
    19081 gcaaccgacg ggatttgcga tcaacctcgc ggaaaggcat ttggcggcgc cgcagaatct
    19141 cgcagcaatg aggatttatt tcgaggccga gccagtcgtg tccggcggcg atcagcctcg
    19201 gcccatacgc gcccgctccg cagccgacat cgacgaccga agcaccggcc ggcagataag
    19261 cctcgattaa attagccggt tcgtcgggag ggttctccac cggcgggccg gagccgtaaa
    19321 tattttcccg gtgcagcagc gtagcgttat ccggatgaac cacgtcaccg tggggttgct
    19381 cgtgcaggcg cgccgggacg agccgcacgc gtcggtcggc gaacgtctgg gccctgcctc
    19441 catcgcagga ggcaacggcg cgcaaagtcg cctcgcgcgc ttccgtcacg gctgccgggg
    19501 ttttgccgag cattcggaag ccggttggga tggaggccga aacgccaagg tgccgcgtga
    19561 catcagaacg gagaaagaaa ttgcgcgttt ccgccacaca ggaatcgtcg agaaacgcgc
    19621 gcacccacca ggtggccgga tcgcgctccg gcacgcaaat ccagcccgag agaaagatcg
    19681 cctgatcata aacactctcg tccggcccgg gtgcatcgag gttgcccgcg aaagttaccc
    19741 ccagggccga ggtctgaagg attcgatcag tcatgcagca gcgtttcaaa cgaaactcgt
    19801 gtcgagactc gaatgtgtca tgcacttttc tggcggacct ggctgggaga agcgacttcc
    19861 aagtcgccct tcgacttcgg gcgggccact caggggtgct cagaactcag caaggcaggg
    19921 tgcgagcatc acggtggctg cggtagcaga ttgatctcgc tctcgcaaac cgcgcggctc
    19981 ggattgcaat ccgaagacgg acgtgtttcc tagaggccag gatgcaaccg ggtcagtggt
    20041 gtcactctag ccgatgcaca atgcgctcca ggccggacgc agtttaaccg aaatgcgccc
    20101 ggaaatcgct ccactcattc gctttgacgc gcgccatcgc gctctcgtgc tcgtcgtggt
    20161 tggtctgttt gtcggttgtg tcatgctgcg cctgcacggc ttttcgctcc cgcaatggca
    20221 cgcccagctt gatggctcgc cgcctaacga gatcctgctc ggccaggcac gcgagattcg
    20281 cgcggacgat tgggcggcgt cgttgtgcac catcctcgcc cagggcgaac accgaccttc
    20341 cttttcagtg ttcaacgaaa acattgggct gggatcgaac atggtggtga gccccatcaa
    20401 gacgccggtg cgtcattaca taaccctctt tcggccacac ttatggggtt actttctcgg
    20461 ccgggatctc ggactcgcct ggcattggtg gttcctgctc ctgggattgt tctacagtta
    20521 tttcctttgt tttttgctcg ttagcggcgg ccgttttctc gtggcgctcg ccggggggct
    20581 gctcgtcgcc tattcgccct tctttcagtt caagagtctg gagtatgcgg agatcgcgat
    20641 ctttgcggcg ctggtctttg tcgctttgat ccaggttctc ttcgcccctt cgcgttggga
    20701 gcgagttgcc gcggctttgc ttctcgcctg gagcgtgtcc gggttcgcct tgaactttta
    20761 tcctccctcc cagatttccc tcggatacct gttgggcttt ctcagtggcg gtttcctgct
    20821 tcgccatcgc gctgcgttgc gtgcgcaggg gcaatgggga caccgtctca tttgtctggt
    20881 tggcagcctt ggtctcgccg cgctggccgt cgggatcttt gtgatgcaag gctgggcgat
    20941 tctacaaatc ctcaaccaga cgatctttcc cggcgggcgg ttcgtcaccg gaggcgatta
    21001 ctcatggtgg cgcgccttcg cgaacaactt cctcctgcag ttctttctca ctgacaatct
    21061 gcccggcacc aacatctgcg agtacggctc gtttttcttt ttctttccgg tcctcgcagg
    21121 gtggatggct tatcggatgt ggcgtgagtg gcaggcggat tacctggttg tgagcatgct
    21181 gcttttctgt tttgctctga tgacatttgc cgttgtcgga ttcccggcct ggctgagcgc
    21241 cgtgacactc atgagcaagg tgccgccaaa ccggagtatg gttgcgctgg ggatagccaa
    21301 tgccgttctt ctctgcgcga tcgtggcttc gccgcaaagc aggaaaccag cggacgctgc
    21361 ggtgctcgtt atcgcaggat tgtggggagt tttcctcttt gcggtggcga gagcgatcgc
    21421 ggaaaactat ccgagtctca gcctcggttt gcttatcctg ccggccgtag cgttcaccat
    21481 tctcggctac ggtttgctcc aggaacgctg gaaggtcacg agcatcctgg tgctggccgc
    21541 gctctcgatt ggcggcacct gttggtttaa tccgctcgtg cgcggtggct ccgcctatct
    21601 ttaccgaaac ccactcgccg ccaagatgcg cgcgctttcc ttgcgcgatc ggcgcgcccg
    21661 ctgggccacc tttggccaga tgttttacgc tgattatccg cgcctgctgg gactgcccgc
    21721 gctcaacggc acacacccgt atccccaatt cgacttgtgg gcgcgcttcg atcccgagcg
    21781 ccgctgggtc gaccaataca accgttacgc ccacgtctgg tttgccaccg ccgcgggcgg
    21841 catcacgatc gagtcaccct ttcttgacca aatcaaggtg tcactctcgc cggccaatcc
    21901 agtcctggag tcgttaggcg tgaaattttt tctcgtcgtt tcacccgagg atcggttctt
    21961 cgaacgccgg ccgcaacagt tctcgaaagt attcagtcag ggccggatcc atatctaccg
    22021 gaggcgcgga gaggaaaaat cgagggcgcc ttgacttcct ggaaaccctc tcattaatca
    22081 gctgccggga gacgaacccc gtcgtgtcga ggatgaccga agcctaatcc agccgccagg
    22141 cgacggtatc gccttcttca gtctccgccc agtcagacga gtgttgcgcg aggaactgat
    22201 gcgcctgcgc gcgctctgcc ggagcaagat ggtggtgctc gtaaagaatg agcttcggct
    22261 ccaaacgcgc gagatcgaat tgtcgcaaga gtcgccaatc ccagccttcg gcatcaatca
    22321 cgaggagatc gaggtgatcc atttgatacc gagccaggag cgtggcgacc gagagacacg
    22381 ggacgacctc ggtcaccgtg ggccgttccg cctggccaaa ttcgcgcgcg ttcctttgca
    22441 gcagcgcgcg atcgagcgag ggaatttgct gcagccagag cgaatcctcg gccgtggttt
    22501 gcaaccgatg gatttcaatc gtgccatcgc gttcgccgat cgcggcccgc tcgaggcgaa
    22561 gcgccgggtc gtggccgtag cgctccgaca gtttggcgaa caaatgcgcc accggttcga
    22621 ccaggactcc gcgccagcgc gtgtccggcc gattgatcaa atgattcaac ggatcgccgg
    22681 tgaagccgtc gttggcgccg atctggagga aaatcgcgtc cggcacggcg gtggcaaaat
    22741 cggcgagcaa ggcgtcggtg tgcgagcgcc ggaaaacgcc ggagcgcagc cggcgagaca
    22801 gaagatgccg tcggagtgtg agactccagc gtcgcgacgg atcattcgtt aggcgcgcgc
    22861 gcccgcgcaa ccaagccgat tgcagcgcgg ccgcgatccg ctgcggaagc gacggccgcg
    22921 ggagcggcgg gggcaatggg tgttcgatct cgaacgtttc gtcaccgagc tggatcgcaa
    22981 gccgcagacc gtctgggccg atctcgcttc ccttgcattt acccgagaag ccgagcgcgg
    23041 tgcgattggg gaaaacgcgc ctcacgtcgg gccgtgcgca agtcgttagg cgttgtttcc
    23101 ccgcggccgg cagccagact gcctgcaccg gccgatcggc cgcgacccag ccggtgaacg
    23161 cgagatccgc atcctcgatc cgatcgcctt tcggatcgag atgc
//
LOCUS       DATMNS010000177         3682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_593984, whole genome shotgun sequence.
ACCESSION   DATMNS010000177 DATMNS010000000
VERSION     DATMNS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_593984"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            109..777
                     /gene="rsmI"
                     /locus_tag="VNW28_06095"
     CDS             109..777
                     /gene="rsmI"
                     /locus_tag="VNW28_06095"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HXA09534.1"
                     /translation="MLYVVGTPIGNLDDITLRALQVLKSVDLIAAEDTRHSGNLLRHF
                     EIRKPLVSYHEHNEAMRTAELVERLATGETVALITDAGMPGLSDPGARLIRKCIERDL
                     PFTIVPGVSAITTALVGSGFGLEHFCYRGFLPIKSGQRERELTAAAGRLETTVFFESP
                     YRINKTLAACAQLMPGRTLCVARELTKKFEEFRRGTAGELLVHYEAHPAKGEITLLVE
                     GAEK"
     gene            968..1312
                     /gene="rplU"
                     /locus_tag="VNW28_06100"
     CDS             968..1312
                     /gene="rplU"
                     /locus_tag="VNW28_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HXA09535.1"
                     /translation="MAYAIIKTGGRQYRVAEGDTIDVDLLEGDAGQKMTFGEVLLHAD
                     GDKMTQGSPLVAGASVSGEVVEQRKDKKVIAFKYRRRKGYHRTVGHRRKLTRVKITGI
                     SLGSKSKKAAAE"
     gene            1341..1598
                     /gene="rpmA"
                     /locus_tag="VNW28_06105"
     CDS             1341..1598
                     /gene="rpmA"
                     /locus_tag="VNW28_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HXA09536.1"
                     /translation="MAHKKGQGSVKNGRDSVSKRLGVKKFGSETVGAGNIIIRQRGTK
                     FLPGKNVGLGRDYTIFALVDGEVRFDRAGRRVNVIPLSPGR"
     gene            1721..2320
                     /locus_tag="VNW28_06110"
     CDS             1721..2320
                     /locus_tag="VNW28_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HXA09537.1"
                     /translation="MKYTTYLLFGAPGSGKGTQGRTLGSIPRFFHCACGDVFRTIDTR
                     TRLGRAFLEYSSRGELVPDDITVELWKARIDAAVDAHQFKPDIDVLILDGIPRNAGQA
                     EIMDTLIDVKKVFHLSCPNREALFARLKKRALKDNRLDDANEEVIKRRLATYEAESKP
                     VLDHYRDRVQMVDATEPPARVLYHILETVANGEHESAAV"
     gene            2301..2549
                     /locus_tag="VNW28_06115"
     CDS             2301..2549
                     /locus_tag="VNW28_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09538.1"
                     /translation="MSQQQFENFTASSLYCEKCKAAMPVRERLLLVLPDREIFDYLCT
                     NCASSVGQREVTAGDKSMARAAAARYPRRSGAPRGVMP"
     gene            2556..3494
                     /gene="fmt"
                     /locus_tag="VNW28_06120"
     CDS             2556..3494
                     /gene="fmt"
                     /locus_tag="VNW28_06120"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HXA09539.1"
                     /translation="MQIVFIGTGEIGVPTLRALRHTPRHQLIGVVTQPDKPVGRDQHF
                     QASPIKAELARSSVPVFQPARIRNDDAVAQIRWLKPDVVVVMAYGQILPKSVLEIPRI
                     ACLNLHASLLPLHRGAAPIQAAIVAGDTGTGITVIYMDEGLDTGDIFLQAKLAISPTE
                     TGGSLHERLAQLAPDALLAALEQLANHDAPRAPQDSSRATYAPKLTREHGKIDWSEPA
                     EMIERKIRAFDPWPGTFTLLVDAAGKPRHLKIFRAAVVPQGGSEPGSITTPNDAEILV
                     ATSRDALRLDEVQLEGKRRMSAAEFLRGFSSPRLHF"
BASE COUNT          730 a         1130 c         1112 g          710 t
ORIGIN      
        1 cgttgatccg ctgaatggtt cttttgtttt tgggactcca actgcggatg cgcgataacg
       61 cgcagccgac ctgttggcgc agcgagggtt cccggcagat aatcggccat gctttacgtc
      121 gtcggcacgc cgattggaaa tctcgacgac atcacgctcc gcgcgcttca ggttctcaag
      181 agcgtggatc ttatcgccgc cgaggatacc cggcattcgg gaaatttact gcggcacttc
      241 gagatccgaa aaccgctggt gagctatcac gagcataatg aggcgatgcg caccgctgag
      301 ttggtcgaac gactcgcgac cggcgagacc gtggcgctga tcacggacgc cggcatgccc
      361 gggttgtcgg acccgggggc gcgcctgatc cgcaaatgca tcgagcggga cttgccattt
      421 acgatcgtgc ctggcgtctc ggccatcacc acggcgctgg tgggctcggg atttggcctc
      481 gagcactttt gttatcgcgg attcctcccg ataaagagcg ggcaacgcga gcgggaattg
      541 accgcggccg cgggccggct cgagaccacg gtatttttcg agtcgcccta tcgcatcaac
      601 aagacgctgg cggcctgcgc gcagttgatg cccggccgga ctctctgcgt ggcgcgcgaa
      661 ttgacgaaga aatttgagga attcaggcgg gggaccgccg gggaattact cgtgcattat
      721 gaggcgcatc ccgccaaggg cgagatcact ttgctcgtcg agggtgcgga gaaatgacgg
      781 atgagcgtca tctggtggtg gctcgttcgg tcttcgtgct tcgtcattga ttcgtgcttc
      841 gtcattcgag attcctcatt tcatttcgga cttcgttatt ccgttgctcc gccggtctga
      901 cagcgccggg atttcctctt gtcaacacgg tcggttcgtg tgactttccc cgacttttcc
      961 caaggccatg gcttacgcaa ttatcaaaac aggcggccga caatatcgcg tcgcggaagg
     1021 tgacacgatc gacgtcgatc tgctcgaggg cgacgcgggg cagaagatga ctttcggcga
     1081 agtgctgctg catgccgatg gcgataaaat gacgcaaggg agccccttgg ttgcaggcgc
     1141 cagcgtgagc ggcgaagtgg tcgagcagcg caaggataaa aaggtcatcg cgttcaagta
     1201 tcgtcgtcgc aaaggttatc accgtacggt cggtcaccgt cgcaaactga cccgggtcaa
     1261 gatcacgggc atcagccttg gctcgaaatc gaagaaggcc gcggccgaat aggcaggcct
     1321 ctcaactctc cactttcctc atggctcata aaaaaggaca aggcagcgtc aagaacggtc
     1381 gcgatagcgt cagcaagcgg ctcggtgtga aaaagtttgg cagcgaaacg gtcggcgccg
     1441 gcaacatcat catccggcag cgcggcacaa aattcttgcc cggtaagaat gtcggcctcg
     1501 gtcgcgacta cacgattttt gccctcgtcg acggcgaggt gcggtttgat cgcgccggtc
     1561 gccgggtcaa cgtcattccg ctgtcgccgg ggcgttgacc ccggctcggg acgagcctgc
     1621 cgggattaac gcccccggcg acaagaggtc aacgcctcgg cgacaacgtt tccgcgttgt
     1681 gctgctcgta gatttcggat ataacgccaa ttccatcgcg atgaaatata ccacctatct
     1741 tctcttcggc gcgcccggca gcggcaaagg cacgcagggt cgtacgctcg gctcaattcc
     1801 ccgctttttt cattgcgcct gcggggatgt cttccgcacg atcgacaccc gcacccggct
     1861 gggtcgggcc tttctcgaat actccagccg cggcgagctc gtgccggacg acatcacggt
     1921 cgaactgtgg aaggcgcgga tcgacgccgc ggtcgatgcg caccaattca agccggacat
     1981 cgatgtcctc atcctcgatg gcattccgcg caatgccggc caggctgaga tcatggacac
     2041 gctaatcgac gtgaagaaag tctttcacct ctcctgcccg aatcgcgagg cgctctttgc
     2101 gcgcttgaag aagcgcgcgc tgaaggacaa tcgcctcgac gatgcgaacg aggaagtgat
     2161 caaacgccgc ctcgcgacct atgaggccga atccaagccg gtcctcgacc attatcgaga
     2221 ccgcgtccag atggtcgacg cgacggagcc cccggctaga gtgctttacc acatcctcga
     2281 gacggtcgcg aacggcgaac atgagtcagc agcagtttga aaatttcacc gcctccagcc
     2341 tctactgcga gaagtgcaag gcggcgatgc cggtgcgcga acgcctcctc ctcgtcttgc
     2401 ccgaccgcga gatcttcgac tatctctgca ccaattgtgc ctcgtccgtt ggtcagcggg
     2461 aagtgaccgc cggcgataaa tcgatggcgc gggccgccgc cgcgcgatac cccaggcgga
     2521 gcggggcgcc gcgcggcgtg atgccgtaag gcggcatgca gattgtcttc atcggcacgg
     2581 gcgaaatcgg cgtgccgacc ctgcgcgccc tgcggcacac accaaggcac caattgattg
     2641 gcgtggtcac gcaacctgat aaaccggtcg gacgcgatca acacttccaa gcttcgccga
     2701 tcaaggcgga attggcgagg tcgtcggtgc cggtttttca gccggcgcgg attcgaaacg
     2761 acgacgccgt cgcgcagatt cgctggctca aacctgatgt cgttgtggtg atggcctatg
     2821 gccaaatcct gccgaagagt gtcctcgaga ttccgcggat cgcctgtttg aatttgcacg
     2881 cctcgcttct tccgctgcat cgtggagccg cgcccatcca ggcggcgatc gtcgcgggcg
     2941 acaccgggac gggaatcacc gtgatctaca tggatgaagg tctcgatacc ggtgacatct
     3001 tcctgcaagc gaagctcgcc atctcgccga cggaaaccgg tggatccctc cacgagcgcc
     3061 tcgcccaact cgcgcccgat gcgttgctcg ccgcgctgga gcaactcgcc aaccacgacg
     3121 ccccgcgcgc cccgcaggat tcctcgcgcg ccacctatgc gcccaagctg acccgcgaac
     3181 acggaaaaat cgattggtca gaaccagccg aaatgattga gcgaaaaatc cgggccttcg
     3241 atccgtggcc ggggacgttt accctgctgg tcgatgcggc gggaaagccg cggcatttga
     3301 aaatcttccg cgccgcagtc gtcccgcagg gtggcagcga gcccggatcc atcactacgc
     3361 ctaacgacgc ggaaatcctc gtcgccacca gccgcgacgc tctccgcctg gacgaagtgc
     3421 aactcgaagg aaagcgccga atgagcgccg ctgagttttt gcgcggtttt tcctccccaa
     3481 gactgcattt ctgatcgatc atcccgccga gatggaaagt gtcacgcccg cctaccaacg
     3541 catcgtcctc aaattaagcg gggaggcgct ccgcgaaacg ggcggacgcg acaccatctc
     3601 gccgcagatt gtgaaggaaa ttgcgcggca ggtgaaggag gtctacgacc tcggcgtgca
     3661 aatttcgatt gtcgtcggcg gc
//
LOCUS       DATMNS010000178         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_594566, whole genome shotgun sequence.
ACCESSION   DATMNS010000178 DATMNS010000000
VERSION     DATMNS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594566"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VNW28_06125"
     CDS             <1..211
                     /locus_tag="VNW28_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09540.1"
                     /translation="LLSLALLGFALMLVPALFALVLRITLTLVLTLPVALRRLTGRAI
                     PRRLVFLRLLRLSGAFRLPVVRRLS"
     gene            829..1437
                     /locus_tag="VNW28_06130"
     CDS             829..1437
                     /locus_tag="VNW28_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09541.1"
                     /translation="MPLIESLKETVEKVTGWVRPSGQEVDEALRRRKPNVFRFADDGF
                     IPNNPNLAFILYRNPVRVTGAPDPAALFEVLFARNGWKNSWRNGIYEYAHYHSQIHEV
                     LGIARGAAMVRFGGSKGRIVKLRAGDVAVLPAGIGHQRLEGDDDFLVVGAYPASGKYD
                     ECRASPEERDRALKTIPRVPLPKKDPVYGSGGPLSRLWLKRV"
     gene            complement(1640..2089)
                     /locus_tag="VNW28_06135"
     CDS             complement(1640..2089)
                     /locus_tag="VNW28_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09542.1"
                     /translation="MRALVLAGRGSLDSAVAQLIADAIRLDLGIAVRCPSLGGLTGIG
                     AAAEAEPDQQPDIVALVSVGAVTSAQLELLLRRASSTFPQSRIVVGYWDGGDPRMQRE
                     GARVRYAESVTSLMDFVGRTAQELSGEQEGKPGAEESARRLEVVTGA"
     gene            2119..>2396
                     /locus_tag="VNW28_06140"
     CDS             2119..>2396
                     /locus_tag="VNW28_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09543.1"
                     /translation="MLALVFQALLHELDIFGDRLVEFINRVAAEDATLAVVGGGSKRR
                     DCDVIEVILEILLRYPAVRVFGSEGRLSCQKLMVKMLVRLENPLVSEQ"
BASE COUNT          442 a          719 c          719 g          516 t
ORIGIN      
        1 cttgctgtct ttggccctgc tgggcttcgc cctgatgctg gttcccgcgc tctttgcgct
       61 ggttctgcgc attaccctga cgttggttct gaccctcccg gtcgcgctgc gtcgattgac
      121 cggtcgtgcg atcccgaggc gtctggtgtt cctgcgcctg cttcgattga gtggcgcctt
      181 ccgattgccg gttgttcggc gattgagctg acgcaagggt cactcccgcc aataacgcgg
      241 tcgttagtag gagtgtcttc ctaattagat ggtcctccat tagtcccctc ttcccctttt
      301 gtgttgaaca gaggaacacg atggccagtt ccgccgctcg ttaaaacttc gtcgcagttt
      361 ggcgcggcag cctgtcgcaa gagaggaaag actcgcgaaa gatcggatct ccgatctttc
      421 agcaccatcg ccgcctaata ctgaaagcag gatacccatc caacgtgtta tggtgatacg
      481 gatggcctga atgcatcgcc cacaagcaac ggttcaagac ctgtcagctt gcgcggaaaa
      541 cgacccccag aagtaaagta attggagggc atgagggcgc atttccgacc cgtccatcac
      601 cggaagaaga cgtgatttct tgataccgcc gacgactgcc ggcgcgccag cacaaggcgc
      661 cggcatgata acgctcgatg tttacttggc cgcttgtggc tgctcggatg ccgagagtgc
      721 ggcgcagggt ggccgggcgt tgtacgactt cgattagatt gatcgccggc ttacggaact
      781 ttgccgccac atgggcattg ggacgacgga gtttggagga atgccgcagt gccgctcatc
      841 gagagcctca aagagacggt agagaaggtt accggctggg tgcggccatc gggccaagag
      901 gtcgacgaag cgctgcgacg ccgaaaaccg aacgtgttcc ggttcgcgga tgacggcttc
      961 attcccaata accccaatct cgcattcatt ctctaccgca atccggtccg ggtaaccggt
     1021 gccccggatc cggccgcact gttcgaagtt ttgttcgctc gcaacgggtg gaaaaactcc
     1081 tggcgtaacg gcatctacga atacgcccac tatcattccc aaattcacga ggtactcgga
     1141 atcgcgcgcg gcgccgcaat ggttcgtttc ggcggctcga aaggtcggat cgtcaagctc
     1201 agggccggcg acgtcgctgt attacctgcg gggataggac atcagcgcct cgaaggggac
     1261 gacgactttc tcgtcgtggg tgcgtatcca gcttccggca aatacgacga atgtcgtgca
     1321 tcgcccgagg aacgcgaccg cgcattgaag accattccca gggtcccgct cccgaaaaag
     1381 gacccggtgt atgggtcagg cggccctctc tcacgcctgt ggttgaaacg agtgtgaagc
     1441 acttttgagt ctgagcccgt gacgcgagat ccgagcggtg ctatctcaaa ggccgtgatg
     1501 gtttccgctt gcagcccaat agatgaagcc gcgcgggggc gttcattccc ttgattcgac
     1561 aacggcaatt cgtcggttct ggagtgcttt cgccggggcc gtgcggacgc cattgtggtt
     1621 ttggcgaaat ctttgcattt caggctcccg ttacgacctc gagtcggcgc gccgactcct
     1681 cggcccccgg tttcccttcc tgctcaccgg acagctcttg cgccgttctg ccgacgaaat
     1741 ccatgagcga agtgaccgat tccgcgtaac gaacgcgtgc gccttctcgt tgcattcgcg
     1801 ggtcgcctcc gtcccaatat ccgacgacga ttcgcgactg cggaaaagtc gagctggccc
     1861 tgcgcagcag aagctcgagc tgtgccgacg tcaccgcgcc gaccgaaacg agcgctacga
     1921 tatctggttg ctggtcaggc tcagcctcgg cagccgcacc gatcccggtc aatcccccga
     1981 gcgaggggca tcggacggcg atgccgagat cgagccggat cgcgtccgcg atcagttgcg
     2041 cgaccgccga atcgaggctt cctcgtccgg cgaggacgag tgccctcacc gagtggcgcg
     2101 ccggctccga tttggacgtt gctcgcgctt gtattccagg ctctcctgca cgagctcgat
     2161 atattcggcg atcgtttggt tgagttcatt aaccgggtcg cggccgagga cgccacgctt
     2221 gcggtcgtcg gaggcggctc gaagcgccgg gattgcgacg tcatcgaggt aattctcgaa
     2281 attctgctcc gttatccagc ggtccgcgtc tttggcagcg aaggtcgcct ctcttgccag
     2341 aaactgatgg taaagatgct cgtgcggctc gagaacccgc tcgtttccga gcagga
//
LOCUS       DATMNS010000179         3458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_594679, whole genome shotgun sequence.
ACCESSION   DATMNS010000179 DATMNS010000000
VERSION     DATMNS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_594679"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..735
                     /locus_tag="VNW28_06145"
     CDS             79..735
                     /locus_tag="VNW28_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXA09544.1"
                     /translation="MPRHPKIPLDGDPDEPGPRLPAVSPQIEPGEPTDLELMHGIQQE
                     DPEALSKLYDRYSGIIKALVLRVVHNEAEADDLLQEIFMELWNQAKNFSAQKGKPLGW
                     MVTLARRRAIDGLRKKQAYLRAGERLQNETEQQPDAWVHNATEEEINLSDTRVLIRQV
                     INSLPPAQQQAIDLAFFRGMSQREIAAKTNTPLGTVKTRLELGLKKIYDGLKELKDEL
                     "
     gene            725..991
                     /locus_tag="VNW28_06150"
     CDS             725..991
                     /locus_tag="VNW28_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09545.1"
                     /translation="MNFKEFQNRARLYVIGALENGESREFEKARAEFGPKAEDFITEC
                     YRLHEAFALSLRPAKSSEALKERLMKMVRARKESQSAGEQPQKH"
     gene            complement(1008..1523)
                     /locus_tag="VNW28_06155"
     CDS             complement(1008..1523)
                     /locus_tag="VNW28_06155"
                     /inference="COORDINATES: protein motif:HMM:NF020989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HXA09546.1"
                     /translation="MDVQRNNGALEISEIDPFLAELLRQIPASTKPDAVPAAERRLFS
                     APAVPDEKELCAEWKVYVEPELRRLFQSATETVESDLRQLNANEKPFANSALRIPHAH
                     SDAWLNALNQARLAIAAKYDFTEAELCDHYRSPIGSRRDLSLFQVNFYGFLQEFILRE
                     MDCDSAAPPDQ"
     gene            complement(1525..1836)
                     /gene="clpS"
                     /locus_tag="VNW28_06160"
     CDS             complement(1525..1836)
                     /gene="clpS"
                     /locus_tag="VNW28_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HXA09547.1"
                     /translation="MHTSQLTTGAVESPAITEEPKSKEALDLPWQVVVHNDPVNLMSY
                     VTMVFQRVFGYPKEKAELHMLEVHRNGRSILWSGVRERAELFVQQLHGYLLLATIERV
                     N"
     gene            complement(1982..2428)
                     /gene="ybeY"
                     /locus_tag="VNW28_06165"
     CDS             complement(1982..2428)
                     /gene="ybeY"
                     /locus_tag="VNW28_06165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HXA09548.1"
                     /translation="MREESISLPEISVRNRQRKIALDRAALERFARRALPLCARERGR
                     GLTNLAEIAVLLISDRKISELHRRFMQIEGPTDVITFQHGEIFISVETAERQAEAHHT
                     SLNHELRLYLVHGLLHLHGFDDRAPAATRRMQAAQERIVLTAGRGF"
     gene            complement(2425..3369)
                     /locus_tag="VNW28_06170"
     CDS             complement(2425..3369)
                     /locus_tag="VNW28_06170"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HXA09549.1"
                     /translation="MRSRHTLPDFRIAIVGAGAIGLYYGGKLAHFGRDVHFLLRRDYA
                     VVRKRGLRIRSKGENIHVPKVQAHRTTEEIGPCNLVIVAVKATSNPQLPTLIAPLLGS
                     ETIILTLQNGLGNEEFLAQHFGAERILGGICFICLNRTAPGVVERFDSGRLALGEFSG
                     YPRPRLHDIAWEFKRCGIVCSVVAALTRERWRKLVWNIPFNGLSVAAGGIDTAAILAD
                     EGLCQLAVQLMDETIAIARARGQNLPTALALEELKRTKAMGEFKPSTLLDYLAGRPLE
                     IEPMWGEPLRRGREAGLESPRLEMLYRLLVALDRGPRR"
BASE COUNT          736 a         1028 c         1054 g          640 t
ORIGIN      
        1 gcatgttttt cgtcgaatgc gtgaaatctg ttggacggga ccgagcgccc gctttagatc
       61 ttctctcttt tgccgcctat gccccgccac ccaaaaattc cgcttgatgg tgaccccgat
      121 gaacccgggc cgcgtctgcc ggccgtgagc ccgcagatcg agccgggaga accgaccgat
      181 ctggaattga tgcacggcat ccagcaggag gatccggagg ccctgtccaa gctttacgat
      241 cgctacagtg ggattatcaa ggcgctcgtc ctgcgcgtcg tgcacaacga ggcggaggca
      301 gacgatttgc tacaagagat cttcatggag ctttggaacc aggcaaagaa tttttccgca
      361 caaaaaggaa aacctctggg ctggatggtg accctggcgc gacgcagggc gatcgatggc
      421 ctgcgcaaga agcaggccta cctgcgcgcc ggggagcggc tgcagaatga aactgagcag
      481 cagcccgacg cctgggtgca caatgcgacc gaggaggaaa ttaacctctc cgatacccgg
      541 gtactcattc gccaagtgat caatagcctg ccaccggcac agcaacaggc gatcgatctc
      601 gcattttttc gcggaatgag tcagcgcgaa atcgccgcaa aaacgaatac accattgggg
      661 accgtcaaaa cgcgcctcga attgggcctg aaaaaaatct acgatggcct gaaagagttg
      721 aaagatgaac tttaaagaat tccagaaccg ggcacggctt tacgtgatcg gcgcgctgga
      781 aaacggcgag agccgtgaat tcgaaaaggc gcgggcggaa ttcggcccga aggcggaaga
      841 tttcatcacc gagtgctacc ggctccacga agcattcgcg ctcagcctgc gaccagcgaa
      901 atcatcggaa gccttgaagg agcgcctgat gaaaatggtg cgtgcgcgca aggagagcca
      961 gtcggccggg gagcaaccgc aaaagcacta ggcaggagcc gaccggatca ctggtcaggc
     1021 ggcgcggcgc tgtcgcaatc catctcacgc aggataaatt cctgcaggaa tccgtagaag
     1081 ttcacctgga acaggctgag atcgcgccgc gaaccaatcg gcgagcggta gtgatcgcag
     1141 agttcggcct cggtaaaatc atacttcgcg gcaatcgcca gtcgcgcctg gttaagcgca
     1201 ttcagccagg catcggaatg tgcatgcgga atccggagag cgctgttggc aaacggcttc
     1261 tcgttggcat tgagctggcg cagatcggat tccacggttt ccgtcgcgct ttgaaagagc
     1321 cgccgcaact ccggctcgac gtaaaccttc cactccgcgc aaagttcctt ttcgtcagga
     1381 acagcggggg cgctgaagag gcgccgctcg gccgccggca ccgcatcggg tttcgtgctg
     1441 gccgggattt gccgcaataa ctccgccaga aaaggatcga tctcgctgat ctcaagcgcg
     1501 ccgttattgc gctggacgtc cattttagtt gacccgctca atcgtcgcca ggaggaggta
     1561 gccgtgcagc tgctggacga agagttcggc ccgttcccgt acgccgctcc acaggatcga
     1621 gcgtccgttg cggtgcactt cgagcatgtg aagttccgcc ttttctttcg gataaccgaa
     1681 gacccgctgg aaaaccatcg tcacgtagct catcaggttg accgggtcgt tatgcaccac
     1741 tacctgccac ggaagatcga gcgcctcctt gctcttgggt tcctccgtaa tcgccggact
     1801 ttcgacggcg ccggtagtca gctgcgaagt gtgcattcga aaaggtgccg gtcgttaggg
     1861 gcgatgtcgc tggcaacgcg aaggcgggcg ggcgaacatt ggcataacca aaagcgtatc
     1921 acacggcagg gttgattcaa ccggcggcgg cctctggcga aacggctctc gcgtccgtcg
     1981 atcagaatcc gcggccggcc gtgagaacaa tcctctcctg cgccgcctgc atccggcgcg
     2041 tcgcagccgg cgcccggtcg tcgaaaccgt ggagatgaag caggccgtgc acgaggtaga
     2101 ggcgtaactc gtgattcagc gaagtgtgat gagcttcggc ctgtcgttcc gcggtttcga
     2161 cgctgatgaa aatctcgccg tgctggaagg tgatcacgtc cgttggtccc tcgatctgca
     2221 tgaagcggcg atgcagttcg ctgatcttgc gatcggaaat caaaagcaca gcgatctccg
     2281 ccaaattcgt taggccgcgg ccccgctccc gggcgcataa cggcagagcc cggcgcgcga
     2341 accgctcgag cgccgcgcga tcgagcgcga tcttccgctg ccggtttcgc accgagattt
     2401 ccgggagtga aatggattcc tctctcatct tcgcggcccg cggtcgagcg caacaagcag
     2461 ccggtaaagc atctccagcc gcgggctttc gaggcccgct tcccggcccc gccggagggg
     2521 ttcaccccac attggttcga tctccagcgg acggccggca agatagtcga ggagggttga
     2581 cggtttgaac tcacccatcg ctttggttcg ctttaattct tcgagcgcga gggccgtcgg
     2641 caggttctgt ccgcgggccc tggcgatcgc gatcgtctcg tccatcaact ggacggcgag
     2701 ctggcacaag ccctcatcgg cgaggatcgc cgccgtgtcg atgccgccag ccgcgaccga
     2761 gaggccgttg aagggaatgt tccagaccag cttgcgccag cgctcgcgtg tgagagccgc
     2821 caccacgctg caaacgatcc cgcagcgttt gaattcccag gcgatgtcgt gcagacgcgg
     2881 acgcgggtag ccgctgaatt ccccgagcgc aagacggccg ctgtcgaaac gctcgaccac
     2941 gcctggcgcg gtccggttga ggcagatgaa acagatcccc ccaagaatcc gttccgcgcc
     3001 gaaatgttgg gcgagaaact cttcgttccc cagcccattc tgcagcgtca ggatgatggt
     3061 ttcgctgccg agcaacggtg cgatcaacgt cgggagctga ggattggagg tagccttgac
     3121 cgccacgatg accaggttgc aggggccaat ttcctctgtc gtgcgatggg cctgcacttt
     3181 cgggacatga atgttctcgc ctttgctccg gatgcgcagc cctcgcttgc gaacgacggc
     3241 gtagtcgcgc cgcaagagaa aatgcacgtc gcgcccgaaa tgcgccagct taccgccgta
     3301 gtaaaggcca atcgcgcccg cgccgacgat ggcaatacgg aaatctggaa gcgtgtgccg
     3361 cgatcgcatc ggaaaaagtt aaccgcagag gacggaaagg acgcagagaa aagacaaatc
     3421 taaaatcctc ctctgcattc tctgcgaact ctgcggta
//
LOCUS       DATMNS010000180         5193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1203221, whole genome shotgun sequence.
ACCESSION   DATMNS010000180 DATMNS010000000
VERSION     DATMNS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1203221"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VNW28_06175"
     CDS             <1..543
                     /locus_tag="VNW28_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09550.1"
                     /translation="SHVTFEELSEMAFDLEAVKVRSIFANHPGICLGYRLSTPSGDIV
                     YMPDHEAYERQESERQKIAGENLPANLDHARAQDEKVIEFIRAADVLIGDTQYDTTEY
                     PGRLGWGHTCADDAVDLAMRAGVKHLFLFHHDPDHHDEKMEAMIARAKENVAAAGSAM
                     MVSAAREGVEFVLEWALPPP"
     gene            complement(687..1292)
                     /gene="recR"
                     /locus_tag="VNW28_06180"
     CDS             complement(687..1292)
                     /gene="recR"
                     /locus_tag="VNW28_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HXA09551.1"
                     /translation="MKRTEYPVAIRELITQLRQMPGVGPRSAERIALWIVQARDEQPA
                     GIARAITSTRDTIRPCRQCGFFATADLCEICADDSRARELVCVVEQPTDILPLEKTSA
                     FRGLYHSLGGRISPLDRVAPEDLRIASLVERVEREAPSEIIFALAADVEGEATTNYLV
                     ALLKEYPVSLTRIAHGLPAGGGLESADELTLSRALSGRTKL"
     gene            complement(1289..2551)
                     /locus_tag="VNW28_06185"
     CDS             complement(1289..2551)
                     /locus_tag="VNW28_06185"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXA09552.1"
                     /translation="MKRALLATLWIGAVAAAVAIVLQTTGLLARPAAFFGKAIGLPPN
                     EVVSFGNFLFVIVLSYAVAWTMLEVPQTWRRVGLLALLFLELIVAAWVWQAAGGSFPP
                     LPAILAASLATALACGFTATDAGRRRSATTRLFERRINQANLDRLTENGALELGEPVA
                     REASLVFCEIANEGELIDELPAAVCAQLTREFNTCANEGFLRDGGYLHAADGEGVRVL
                     FGFPNASEQHARDAARAALAFRDSFRAAAAAKPDSLGRIDLRIGLSSGVIVATRRDDS
                     PRSEIVVSGESLEVARRLARANQVYGSQILLGPKTYSAAGKEIVARPIDFLRSSESHE
                     RLEVYELLSLAEQATAEEIARRDRFWTAIVYFRERRWNEAFAEFTRARSENGELDRPL
                     QWYLRRLEPLCLRMATEPAPGSESIAPF"
     gene            complement(2548..4074)
                     /locus_tag="VNW28_06190"
     CDS             complement(2548..4074)
                     /locus_tag="VNW28_06190"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXA09553.1"
                     /translation="MKSNFGDKVSFATFAQPEQAEAGDDEKPAKASDEEFVFKFLPRD
                     IKAHLDRFVIKQDEAKKVLSIAVCDHYNHVNYVRQLESEDAVRAEETEYAKQNVILVG
                     PTGVGKTYLIKHIAELIKVPFVKADATKFSETGYVGGDVEDLVRELVQKADGDVALAQ
                     FGIIYIDEVDKLASASNLVGRDVSGRGVQTTLLKLMEETEVPLRSANDLQGQLQAAFE
                     FQKRGKAKRETISTRHILFVVSGAFERLKTQVARRLAQGQIGFQSEPLKVLDNDVFKH
                     VGTQDFIEYGFEPEFIGRLPVRVVCEELTADDLYKIMKYSEGSILRQYERAFRAYGIE
                     ISFEDEALLLIANAAALEKTGARGLLTVWEKLFRDYKYYLAGSGLSQLRVTEELVREP
                     QRVLERLMAEGQQHEKVALMQEARMFADRFSNEHGVELQFDDSALRLLVERAEAERMK
                     MSDLCAHLFKDFQFGLSLIKKNTGKNQFVLDATAVEAPDKFLSELVLQSYGPGERPAK
                     "
     gene            4391..4714
                     /gene="yajC"
                     /locus_tag="VNW28_06195"
     CDS             4391..4714
                     /gene="yajC"
                     /locus_tag="VNW28_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXA09554.1"
                     /translation="MLITLLAQAPTATPAGAGPNPLTNFIPIILIFIIMYFLLFRPQM
                     RRQKEQRNLVAALKTGDRVVTASGIHGMITNVKDATVTVKVADNVKLEMDKSAVTNVI
                     KTVEG"
     gene            4718..>5193
                     /locus_tag="VNW28_06200"
     CDS             4718..>5193
                     /locus_tag="VNW28_06200"
                     /inference="COORDINATES: protein motif:HMM:NF045451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09555.1"
                     /translation="MTPAITFFIGLVLLVLFGWYFATDFGPRKRLLATLLMVLLVVFS
                     IMTIWPPSKKVQLGLDIQGGTSFLIRLMQGDRPLTKTNLDQAVEVIRKRVDYFGGGEP
                     VISPVGTDRILVQIPGLDPAKIIEARQQLSRVAKLEFRLVYPDNGERLRAIDAGTEV"
BASE COUNT          998 a         1578 c         1615 g         1002 t
ORIGIN      
        1 agccacgtca cttttgagga actgagcgag atggcgttcg atctcgaggc ggtgaaggtg
       61 cgctcaatct tcgccaacca tcccggcatt tgccttggct accggctgag cacgcccagc
      121 ggcgacatcg tctacatgcc cgaccacgag gcctacgagc gacaggaaag cgagcggcaa
      181 aaaattgccg gcgaaaattt gccagcgaac ctggatcacg cgcgcgccca ggatgaaaag
      241 gtgatcgagt tcatccgcgc ggccgacgtc ctgatcggcg acacgcaata cgacacaacc
      301 gaatacccgg gccgcctcgg ctggggacac acttgcgccg atgatgcggt tgatctcgca
      361 atgcgcgccg gggtgaaaca cttattcttg ttccatcacg atcccgatca tcacgacgag
      421 aaaatggagg caatgatcgc gcgcgcgaaa gagaatgtcg ccgcggcggg ctcagcgatg
      481 atggtcagcg cggcgcgcga aggcgtggag tttgttctcg aatgggcgct gcctccgccc
      541 taacgaatac gagtgccagg cctcaatgaa accgaaaccc attgttgggc tgacgaagga
      601 aatttttttg ctcttgcagg gggcgatgac cgcggccggg ctgtgagcga cccgaaccgc
      661 agcggtctgg gacaggtgga tgggtgctac agttttgtcc ggccggagag cgcgcgcgag
      721 agcgtcaatt catcggcgga ttccaatccg ccgccagcgg gcaggccgtg cgcgatgcgg
      781 gtcagcgaga cgggatactc cttcaatagc gcgacgagat agttggtcgt cgcctcccct
      841 tccacgtcgg ctgcgagcgc gaagatgatc tcgctcggcg cctccctttc cacccgctcg
      901 accaacgagg cgatgcgcag gtcttccggc gcaacccgat cgagcggcga aatgcgtccg
      961 cctaacgagt ggtagagacc gcgaaaggcg ctcgtctttt ccagcggcag aatgtcagtc
     1021 ggctgctcga cgacgcaaac aagctcgcgc gcgcgcgaat catcggcgca aatttcgcag
     1081 aggtcggcgg tcgcgaagaa gccgcactgg cgacaggggc gaatggtgtc gcgcgtgctc
     1141 gtgatcgcgc gcgcgatccc ggccggctgc tcatcccgcg cctgcacgat ccagagggcg
     1201 atgcgctcgg cggagcgcgg accgaccccg ggcatctggc gcaattgggt gatcaactcg
     1261 cggatggcga cgggatattc ggtgcgtttc aaaacggcgc gatcgattcg gagcctggcg
     1321 cgggttcggt cgccatccgc agacaaagtg gctcgaggcg gcggagatac cattggaggg
     1381 gccggtccag ttcgccgttt tcgctccgcg cccgggtaaa ttccgcgaag gcttcgttcc
     1441 agcggcgctc gcgaaaataa acgatcgcgg tccagaaacg gtcccggcgc gcgatttctt
     1501 ccgcggtcgc ctgctcggcc agcgagagca gttcgtagac ttcgagccgc tcgtgcgact
     1561 cggagctgcg caaaaaatcg atcggtcgcg cgacgatttc cttgcccgcc gcgctgtagg
     1621 tcttgggtcc aagcaatatt tgcgagccgt aaacctggtt cgcgcgcgcc agccggcgag
     1681 cgacctcgag ggactcgccg gaaacgacga tctcactgcg cggtgaatcg tcccgtctcg
     1741 tcgccacgat gaccccggag ctgaggccga tgcggaggtc gattctgcct aacgaatccg
     1801 gcttcgcggc cgccgccgcg cggaagctgt cccggaaggc gagcgcggca cgggccgcat
     1861 cgcgcgcatg ctgctcactc gcgttgggaa aaccaaaaag gacgcggacg ccttcaccat
     1921 ccgcggcgtg caggtaaccg ccatcccgca ggaagccctc gttcgcgcag gtgttaaact
     1981 cccgggtcaa ctgcgcacag accgcagccg ggagctcgtc gatcagctcg ccttcattgg
     2041 cgatctcgca aaagacaagg ctcgcttcgc gcgccaccgg ctcgcccaat tccagcgccc
     2101 cattttccgt cagacgatca aggttcgcct ggttgatgcg tctctcgaag aggcgcgtgg
     2161 ttgcgctgcg ccggcgaccg gcatccgtcg ccgtgaagcc gcaggcgagt gccgttgcga
     2221 ggctggccgc gaggatggcg ggcagcggcg gaaaggaacc tccggcggct tgccaaaccc
     2281 aggccgcgac aatcaactcc aggaaaagca gggccaggag accgacgcgc cgccacgtct
     2341 ggggcacctc cagcatggtc cacgcgaccg cgtagctgag cacgatgacg aaaagaaaat
     2401 ttccgaaact gacgacctcg ttaggcggga gaccgatggc tttgccgaaa aatgcggcgg
     2461 gacgggcgag gaggccggtc gtctgcagga cgatggcaac ggcggcggcg accgcgccaa
     2521 tccaaagtgt ggcgaggagg gcgcgcttca tttcgccggg cgctcacccg gtccgtagga
     2581 ttgcaggacc aattcactca ggaatttatc cggagcctcg accgccgtgg catcgagcac
     2641 gaactggttt ttacccgtgt tctttttgat caagctgaga ccgaactgaa aatccttgaa
     2701 aagatgcgcg cagagatcgc tcattttcat ccgctccgct tcggcgcgct cgaccagcag
     2761 gcgcagcgcg gagtcgtcaa actgcagttc gaccccgtgc tcgttggaaa agcgatcggc
     2821 aaacatcctg gcctcctgca tgagcgcgac tttttcgtgc tgttgaccct cggccataag
     2881 ccgctcgagc acacgctgcg gttcgcgtac cagttcttcg gtcacgcgca gctggctgag
     2941 gccggagcct gcgaggtagt acttgtaatc gcggaataat ttttcccaga cggtgagcag
     3001 gccgcgcgcg ccggttttct cgagggccgc cgcgttcgcg atgaggagca acgcttcgtc
     3061 ctcgaaacta atctcgatgc cataggcgcg gaatgcacgc tcgtactgcc gcaggatgct
     3121 gccctcggaa tatttcatga tcttgtagag atcatccgcg gtcagctctt cgcaaacgac
     3181 ccggaccggg agccgtccga tgaactccgg ctcaaagccg tactcaatga agtcctgggt
     3241 gccgacgtgc ttgaagacgt cgttgtcgag gaccttgagg ggctccgatt gaaaaccgat
     3301 ctgcccctgc gcgagacgac gcgcgacctg cgtcttgaga cgctcgaagg caccgctgac
     3361 gacaaacagg atatggcggg tgctgatcgt ttcgcgcttg gccttgcccc gcttctggaa
     3421 ttcgaaggcg gcctggagct gtccttgcag atcgtttgcg ctccgcaacg gcacttcggt
     3481 ttcctccatc agtttgagca gcgtcgtctg cacgccgcgg ccgctgacgt cgcgcccgac
     3541 caaattcgaa gcagaggcca gcttgtcgac ttcgtcgatg tagatgatgc cgaactgcgc
     3601 gagcgcgaca tcgccatcag ctttctggac cagttcccgc acgagatctt ccacatcgcc
     3661 accgacgtag ccagtctcgc tgaacttggt cgcgtcggcc ttgacgaagg gcaccttgat
     3721 cagctccgcg atgtgcttga tcaggtaagt ttttccgacg ccggtcgggc cgacgaggat
     3781 gacgttttgc ttggcgtatt cggtttcctc ggcgcggacg gcgtcttcgc tctcgagctg
     3841 gcggacgtaa ttgacgtggt tgtaatgatc gcagacggcg atcgatagga ccttctttgc
     3901 ttcgtcctgc ttgatcacga aacggtcgag gtgcgccttg atgtcgcgcg gcagaaactt
     3961 gaaaacgaac tcctcatcgc tcgctttcgc cggtttctca tcgtcgccgg cctccgcttg
     4021 ttcgggctgg gcaaaggtcg cgaacgaaac tttgtcacca aagttcgatt tcataaattc
     4081 ggagagcttg gcctggagtt cttcgggcgt gggaaatggc gggttttgat tcaagggcga
     4141 aacttaacat cgtcgtttgg tatgtaaaat ttgctgcgtc tgtaagacgt ttcgctctgc
     4201 gacgcgcggg aaccggcgtt ctgcgcgagc cgagtaacct cgcgtttcgc agagcgaaac
     4261 gtcgatcggt ccggcgtcgc gtcacgcttg cgtcccttgc atttcgagcg caactgtgct
     4321 cagtggaatg ccgctccggc gggccggctg cgcccgaggc gcatccgccg ctgcgccaac
     4381 tcatttgacc atgctcatca cccttctcgc tcaggccccg accgcgacgc ccgccggcgc
     4441 cgggcctaat ccgctcacca acttcatccc gatcattctg attttcatca ttatgtactt
     4501 tctgcttttc cggccgcaga tgcgccggca gaaggaacaa cggaatctgg tggcggcgtt
     4561 aaaaactggc gaccgggtgg tgaccgcatc tggaattcac gggatgatta cgaatgtgaa
     4621 ggacgccacc gtgacggtga aagtcgcaga caacgtgaag ctcgagatgg ataaatccgc
     4681 ggtcaccaat gtgatcaaaa cggtggaggg ttaagcgatg actccagcca ttaccttttt
     4741 catcgggctc gtcttgctcg tgcttttcgg ctggtatttc gcaaccgatt tcgggccgcg
     4801 gaaacgcctg ctggcgaccc tgctcatggt gctgctggtc gtcttcagca tcatgaccat
     4861 ctggccgccg agcaagaagg tgcaactggg gctcgatatt caaggtggca cttccttcct
     4921 catccgcctg atgcaggggg atcggccgct gacgaagact aatctcgacc aggccgtcga
     4981 ggtcatccgc aagcgcgtcg attacttcgg tggcggagag cctgtgatca gcccagtcgg
     5041 cacggatcga atcctggtgc aaatccccgg gctggatccg gccaagatca tcgaagcgcg
     5101 ccaacagctc tcccgcgtcg cgaagctcga gttccggctg gtttatccgg ataatgggga
     5161 acgcctgcgc gcgatcgacg ccgggacgga agt
//
LOCUS       DATMNS010000181         6239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3347141, whole genome shotgun sequence.
ACCESSION   DATMNS010000181 DATMNS010000000
VERSION     DATMNS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3347141"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1295)
                     /locus_tag="VNW28_06205"
     CDS             complement(72..1295)
                     /locus_tag="VNW28_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXA09556.1"
                     /translation="MIYRARTLITMDGPPIDNGAVAVVDDRIADVGRSDEVRGRQRGA
                     VTDLGEVVLLPGLINAHCHLDFTSLRGTIAPQRSFAEWIRQINDRRRELSEEDFLHSI
                     TDGFAEAQRWGTTTIANIESMPELLQRMAPPPLRTWWFAELIDVRPTLAAEEMIENAL
                     ALFRGKENWPGSFGLSPHAPYTVSPKLLRLAARAGEQSDMLLTMHLAESEEEMEMFRA
                     GGGPLFDLLQSLGRPMDDCGRGKTSLATILDRQTIDERWILVHLNELTEEDFERVAVG
                     PRFHIAHCPRSSRYFQHRPFALRRLRDLGFNVCLGTDSLASNSSLSLFSEMQAVRDAH
                     PWLAPREILEIATVNGARALHQEETLGKIRVGAQADLIALPIDNNGGIFEKIVAWSDP
                     VSWLLVAGRAPSDNL"
     gene            complement(1456..2118)
                     /locus_tag="VNW28_06210"
     CDS             complement(1456..2118)
                     /locus_tag="VNW28_06210"
                     /inference="COORDINATES: protein motif:HMM:NF036541.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DR2241 family protein"
                     /protein_id="HXA09557.1"
                     /translation="MLPRAERALRDWLASGARGIGQLLIVKTDGFSLRHREDEARADL
                     QVFYSPNDATELARQDDAGNYRPLKTAPNLRHGWVLELPDLPALRLALDLFYPGRLAA
                     LAAWEANQLATTPLRATLDRQTGMYRVAAKISDGEIDELVGRVCRSRGGEPGCLRTIL
                     WARDASGARPSLRLPLDKFIAPVDQTERGEHIIPLLCQEACNLLVAEARKVVKESSSR
                     AE"
     gene            complement(2102..2596)
                     /locus_tag="VNW28_06215"
     CDS             complement(2102..2596)
                     /locus_tag="VNW28_06215"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXA09558.1"
                     /translation="MKNNSTKLLQDLAAFLLAAPKNPATIKKAAEMVRAERSYRWVGV
                     YKLERNEFVIAAGTGDEPPTYARFPMGQGLCGAVAESRETLIVGDVRKDPRWLPAFWN
                     TQSEIVVPIIGESSGKLLGMLDVESDKLDAFDEDDRDFLEHVAVLLAGKLCTAKKHRH
                     VAAR"
     gene            complement(2619..3455)
                     /locus_tag="VNW28_06220"
     CDS             complement(2619..3455)
                     /locus_tag="VNW28_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HXA09559.1"
                     /translation="MDWQQPQSALLIVGHGSTVNPDSSAPSLAQAATIRDRGVFPEVA
                     CCFWKEEPSLRDALLFFREAEIREVYVVPNFISEGYFTETVIPRELELEGRLTTRPDG
                     QVWKYCEPVGNHPAVTELLLRRAREVAPGVAEAETTLLIVGHGTALNENSAVAAKREV
                     GKISALGAYASVQNAYMEEAPLISDWARLTTTPYVVVVPFFISDGLHSYQDIPVLLGI
                     ESGTTAAASQREIFRQNPHRIYGRDLYYASAIGTEPKFAEIIVEQAAAFDTSALDLSP
                     IA"
     gene            complement(3518..5413)
                     /gene="mrdA"
                     /locus_tag="VNW28_06225"
     CDS             complement(3518..5413)
                     /gene="mrdA"
                     /locus_tag="VNW28_06225"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXA09560.1"
                     /translation="MFFPEIGAPLRHLRMILPLCASLACAQQPVENPPTAPTASPLEE
                     TIKPTFETQKQARTYLLDIPAPRGQITDRNGVPLAQNKLSYNLTIDFPTPLNLSDARA
                     LEFARTRIKIAERALARSLKIPDAAILRHYHNRGFLPLEIAQDLSEAERTKLPARLSS
                     GLSLKPLYIRFYPNESVAGQIVGYTGKTGRNADGIIQNGQVLWPETEGREGLEQTFNS
                     MLTGRHGKYKVTFDRDGRKTSERIVEPPVPGYNVVTTLDLHLQEMAEKALAAKAKRGA
                     IVIMDPNNGDILALASWPTFDPNEFIPSISAAKFQELQNDPDIPLLPRAYRSAYPPGS
                     TFKIAVGLAAFQSGTITTDDQYECVPEMNVGNTLFHNWKHKDRGSLNFVQALTESCDT
                     WFYQVGIRTGAGPILDWATRLGFGAKSGIPLKGEAEGRLPNDAYMKATHGRKILGGDI
                     ANLSIGQGDLLVTPLQMAQAMAIVANGGTLYQTRLVEQVQTVSNEIVAAYSVREKRTI
                     DASALTMKQIHTAMVDAVNAGAGTAHQASLDKVSVAGKTGTAQWGPKEKERTAAWFAG
                     FVPADHPQYAFAALYEGEVGSKVHGGSAAAPMIGKILRQLYNEKGEEEKPVKAEKAEP
                     EETDESD"
     gene            complement(5461..>6239)
                     /locus_tag="VNW28_06230"
     CDS             complement(5461..>6239)
                     /locus_tag="VNW28_06230"
                     /inference="COORDINATES: protein motif:HMM:NF020048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXA09561.1"
                     /translation="ISNISTTHVVASMVSFRDGVPDKNNYRRYRIRTVKGQDDFASMA
                     EVVRRRYARVLLQARAAYSDAAEFSQENPVEALARHPERSLVKSKDPGESSAGSATGS
                     LDSARDDNFVAVRLPDLIIVDGGKGQLSSACRELQRLGLHELPIIGLAKEFEEIYRPG
                     RALPLQLPPDSGALRLLQRIRDEAHRFANTYHQLLMKKRIGESLLDDCPGVSQNRKNL
                     LLRKFGSVQRLRKASVEEIASTEGIGQKLAEEVHRFLEQH"
BASE COUNT         1118 a         1973 c         1911 g         1237 t
ORIGIN      
        1 ccggctggcg aatcgcagtt ttgtccttcg cgaaaattgc aaaccgcgga ggaaggaatc
       61 agcgcctccg gctacagatt atcgctcggg gcgcgaccgg cgacgagcag ccatgagacc
      121 gggtcgctcc aggcgacaat cttttcgaag atgccgccgt tgttatcaat cggcagcgcg
      181 atcagatcgg cctgagcgcc gacgcggatt ttgcccagtg tctcctcttg atggagcgcg
      241 cgcgcgccgt tgacggtcgc gatttccaaa atttctcgtg gtgcgagcca cggatgcgcg
      301 tcgcgcacag cttgcatttc cgagaaaaga ctgagcgaag aattgctcgc gaggctgtcg
      361 gtcccaaggc agacgttgaa gcccagatcg cgcagcctgc gcagcgcgaa gggccggtgc
      421 tgaaaatagc ggctgctgcg cggacagtgc gcgatgtgga agcgcggtcc gaccgcgacc
      481 cgctcgaaat cttcctccgt gagctcattc agatgcacca agatccagcg ctcgtcgatc
      541 gtctgccggt cgaggatcgt cgccagcgat gtttttcccc ggccgcaatc gtccatcggt
      601 cggccgagac tttgcagcag atcgaacaac ggtcctccgc cggcgcgaaa catttccatc
      661 tcttcctctg actcggcgag gtgcatcgtg agcagcatgt cgctctgctc acccgccctc
      721 gcggcgaggc gcaataactt cggcgagacc gtatagggag cgtgcgggct caagccgaag
      781 ctgcccggcc aattctcttt gccccggaaa agcgcgagcg cattctcgat catctcctcg
      841 gccgccaagg tcggccgcac gtcgatcagt tcggcaaacc accacgttcg caatggcggc
      901 ggcgccattc gttgcaggag ttccggcatc gactcaatgt ttgcgatcgt tgttgtgccc
      961 cagcgttgcg cctccgcgaa accgtccgtg atcgaatgga gaaaatcctc ctccgaaagt
     1021 tcgcgacgcc ggtcgttgat ctgccggatc cattcggcaa acgatcgttg cggcgcgatc
     1081 gtgccgcgca gcgacgtgaa atcgagatga caatgcgcgt tgatcaggcc gggcaataga
     1141 accacttcgc ccagatcggt cacagcgcct ctctggcgtc cccgcacttc atcggagcgc
     1201 cccacgtccg cgatccggtc gtcgacgacc gcgaccgcgc cgttgtcgat cggcgggccg
     1261 tccatcgtga ttaaggttcg ggcgcggtag atcaaaattt ccgtcctccc gagcgcagtc
     1321 gaggggtccc ggggcgcgat cgttccggcg cctcagcgag atcccgaggc gcaacgactc
     1381 cggtagttcc aagatggcgg ggcgcgactc ttccggttac cccagcggga tccctcgact
     1441 ccgttgcgct ccgcttcact ccgctcggga tgacgactcc ttcaccacct tgcgcgcctc
     1501 ggccacgagc aggttgcacg cttcctggca aaggagcggt atgatgtgtt cgccccgctc
     1561 ggtttggtcc acgggcgcaa tgaatttatc gagcggcagg cggagcgacg gccgcgcccc
     1621 gctcgcgtcc cgcgcccaga gaatcgtccg caaacatccc ggctcgccgc cgcgcgagcg
     1681 acaaacgcgc ccgaccaatt cgtcgatttc gccatccgaa atcttcgccg ccacgcgata
     1741 cattccggtt tgccggtcga gcgtggcgcg cagcggcgtc gtcgccagct ggttcgcctc
     1801 ccaggccgcc agcgccgcca gccggccggg gtaaaagaga tcgagcgcga gccgcagggc
     1861 aggcaaatca gggagctcga gaacccagcc atgccggaga ttcggcgcgg tctttagcgg
     1921 acgatagttg cccgcgtcgt cctgccgcgc caattccgta gcgtcgttag gggaataaaa
     1981 aacctgcagg tccgcgcgcg cctcgtcttc gcgatgccgc agcgagaatc cgtcggtctt
     2041 gacgatcagc agttgcccga tgccgcgggc gccggatgca agccaatcgc gcagtgcccg
     2101 ctcagcgcgc ggcaacatgg cggtgctttt tcgccgtgca cagtttcccc gccaggagaa
     2161 cggcgacgtg ctcgaggaaa tcccgatcgt cttcgtcgaa cgcgtccagt ttgtcgctct
     2221 cgacatccag catgccgagg agtttcccgc tcgattctcc gatgatcggc accacgatct
     2281 cggactgagt gttccagaag gccggcagcc agcgcgggtc tttgcggacg tcgccgacga
     2341 tgagggtctc gcgcgattcc gccaccgcgc cgcaaagtcc ctgccccatc gggaaccgcg
     2401 cataggtcgg tggttcatcc ccggttccag cggcgatcac gaattcgttg cgttcaagct
     2461 tgtagacgcc gacccagcga taggagcgtt cggcgcggac catttccgcc gccttcttaa
     2521 tggtggctgg attcttcggc gcggccaaaa gaaaggccgc caaatcctgc aggagcttgg
     2581 tcgagttgtt tttcaaagta acggttacct agaacaattt acgcaatcgg gctgagatca
     2641 agggctgagg tgtcgaaagc tgccgcctgc tccacgatga tctcggcaaa tttcggctcg
     2701 gtcccgatcg cgctcgcgta ataaaggtcg cgtccgtaga tgcggtgcgg gttttgacgg
     2761 aagatctcgc gctgactcgc agcggcggtc gttccgcttt cgatcccgag caaaacggga
     2821 atgtcctggt agctgtggag cccgtcggaa ataaaaaacg gcacgaccac gacatacggt
     2881 gtcgtcgtga ggcgggccca atccgaaatg agcggcgctt cctccatgta ggcgttctgc
     2941 accgaggcgt aggcgcccag cgcgctgatt tttcccacct cgcgtttcgc cgccacggcg
     3001 ctgttctcat tcaaggccgt cccgtgaccg acgatgagca acgtcgtctc ggcctctgcc
     3061 acgccggggg cgacctcgcg cgcgcgccgc agcaaaagct ccgttacggc tgggtgattt
     3121 ccgaccggct cacaatattt ccagacctga ccgtcgggcc gcgtcgtcag gcgcccctcc
     3181 agctcgagtt cccgcggaat gacagtctcg gtaaaatatc cttcgctgat gaaattcggc
     3241 accacgtaaa cctcgcgaat ctcggcttcg cgaaaaaaaa ggagggcgtc gcggaggctc
     3301 ggctcctctt tccagaagca gcaggcgacc tcgggaaaca ctccgcggtc ccggatcgtc
     3361 gccgcctgcg ccaggctcgg cgcgcttgaa tccggattca ccgtcgagcc gtgaccgacg
     3421 atgagaagag ccgattgcgg ctgctgccaa tccatgagga ggagcgtaag tcactcccgc
     3481 gcccgacgaa agtgaccccg ttttgtcttc cgtttcgtta gtccgactcg tccgtctctt
     3541 cgggctctgc tttctctgct ttcactggct tctcctcttc gcccttctcg ttgtagagct
     3601 gccgcaggat tttcccgatc atcggcgccg cggccgaacc gccgtgaact ttgctgccga
     3661 cttcgccctc gtagagcgcg gcaaacgcat attgcggatg gtccgccggg acgaagccgg
     3721 caaaccacgc cgccgtccgc tccttttcct tcgggcccca ttgcgcggtg ccggttttcc
     3781 cggccaccga caccttatcc aggctggcct gatgcgcggt gccggccccg gcgttgacgg
     3841 catcgaccat cgcggtgtga atctgcttca tcgttagagc ggaggcatcg atcgtcctct
     3901 tctcgcgcac ggaataggcc gccacgattt cgttgctcac cgtctgcacc tgttccacca
     3961 ggcgcgtctg atataaggtc cccccattgg ccacgatcgc catcgcctgc gccatctgca
     4021 gcggggtcac gagcaggtcg ccctgtccga tcgagaggtt ggcaatatcg cctccgagaa
     4081 tttttcgtcc gtgggtcgcc ttcatgtatg cgtcgttagg caaccgtccc tccgcttcgc
     4141 ctttcagcgg aatcccgctc ttggcgccga agccgagtcg cgtcgcccaa tcgagaatcg
     4201 gccccgcgcc agtccggatg cccacctggt aaaaccaggt gtcgcacgat tcggtcagcg
     4261 cctggacgaa attgagcgag ccgcgatcct tgtgtttcca gttgtggaaa agcgtgttgc
     4321 cgacattcat ctccggcacg cattcgtatt gatcgtccgt tgtgatggtg ccgctttgaa
     4381 aagcggccag gcccaccgcg attttaaagg tggatcccgg gggataagcc gaacggtagg
     4441 cgcgcggaag gagcggaata tcgggatcgt tctgcaactc ctggaacttg gccgccgaga
     4501 tcgatggaat gaattcgtta gggtcaaagg tcggccagga ggcgagggcg aggatgtcgc
     4561 cgttgttcgg atccattatc acgatcgcgc cgcgcttggc tttagccgcg agcgctttct
     4621 ccgccatctc ctggaggtgc aaatcgagcg tcgtgacgac gttgtagcct gggaccggcg
     4681 gctcgacgat ccgctccgac gttttccggc cgtcgcggtc gaaggtcact ttgtacttcc
     4741 cgtgccggcc ggtcagcatg ctgttgaagg tctgctccag gccttcccgg ccctcggttt
     4801 ccggccagag cacctgccca ttctgaatga tgccgtccgc attgcgacct gtcttgccag
     4861 tgtatccgac gatctgcccc gccacgcttt cgttagggta aaagcgaatg tagagcggct
     4921 tgagcgagag cccgctggaa agcctggccg gcaacttcgt ccgttccgct tcgctcaggt
     4981 cctgggcgat ctccaggggc agaaaccccc ggttatgata gtggcgcaga atggcggcat
     5041 cggggatttt gagcgagcgg gcgagcgctc tctcggcgat cttgatccgc gtgcgcgcaa
     5101 actccagcgc ccgggcgtcg gacaaattga gcggggtggg aaaatcaatc gtgaggttgt
     5161 agctcagctt gttttgcgcg agcggcacgc cattgcgatc ggtgatctgg ccgcgcggcg
     5221 cggggatatc gagcaggtag gtccgcgcct gcttctgcgt ttcgaaagtc ggcttgatcg
     5281 tttcctccaa gggcgacgcc gtcggcgcgg tcggcgggtt ttccacgggt tgctgggcgc
     5341 aggccagcga cgcacagagg ggaagaatca tgcgcagatg acgcagcggc gcgccgattt
     5401 caggaaagaa cacgggtgtg gaaaatttca atcggcgtca atctgcgata accgcgaatg
     5461 ctagtgctgc tccaaaaagc gatgcacttc ctccgccaac ttctggccta ttccctcggt
     5521 cgacgcaatc tcttcgacgc tcgccttgcg caggcgttgc acagaaccaa atttgcgcag
     5581 gagaagattc ttgcggtttt ggctcacgcc cgggcagtcg tcgagcaggc tttcgccgat
     5641 ccgttttttc atcaggagct gatgataagt gttggcgaac cgatgcgctt cgtcgcgaat
     5701 gcgctggagg agacgcaacg cgccggaatc ggggggcaac tgcagcggga gcgcgcggcc
     5761 gggccggtag atttcctcga actctttggc gaggccgatg atcggaagct catgcaagcc
     5821 gagccgctgc aactcgcggc aggcggacga gagctggcct ttgcccccgt cgacgatgat
     5881 gagatcgggc aagcgcaccg ccacgaaatt gtcatcccga gcggagtcga gggatcccgt
     5941 ggcggagcct gcagatgact ccccgggatc cttcgacttc accaagctcc gctcgggatg
     6001 acgggcgagc gcttccactg ggttttcctg cgaaaattcc gccgcgtccg aataggccgc
     6061 gcgcgcctgc aggaggaccc gcgcataacg acgcctaacg acctcggcca tgctcgcgaa
     6121 atcatcctgg cctttgaccg tccggatgcg gtaacgccgg taattattct tgtccggaac
     6181 gccgtcgcgg aaggaaacca tcgaagcgac tacatgcgtg gtggagatat tcgagatgt
//
LOCUS       DATMNS010000182         5338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3347736, whole genome shotgun sequence.
ACCESSION   DATMNS010000182 DATMNS010000000
VERSION     DATMNS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3347736"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1856)
                     /locus_tag="VNW28_06235"
     CDS             complement(<1..1856)
                     /locus_tag="VNW28_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXA09562.1"
                     /translation="MAEKIAFDHYEVLTREDGSLFELGRGAMGITYKAFDTNLRVNVA
                     LKVINAKFLESEIAQQRFLREARAAAQLRHPNVASVFHLGTSSEAFFYAMEFVDGETV
                     ESFIKRQGPIDPVIALRITLQVARALAAAEKHHLVHRDIKPANLMLVREDDDFLVKVI
                     DFGLAKSIKHEGNDDLATLSLGGFVGTAHFASPEQLEEKEIDVRSDIYSLGVTLWYML
                     VGEAPFGGSLAQVMSQHLHKPPPLEKLQNLPASVRALVGNMIEKDPARRPQTPAALRA
                     ALEKCIADIQAAPQRGEQDFPTVVEAPPPPLPVEVPPAPVRKSSRMMIWALAAVVLTG
                     AGALIFLTTRKTEKILPPTQASSSAAPSPVASATPAISQATAAPSARSVSPAASLSGQ
                     ELSRTSLAAAEKYEAAQDWPRAIMAYVQLQKDFPESEVGRVRLELLLSRLQSEKGALR
                     EDNFEQLRAPLTAAAKLNVVSAMEILGDFLRKRDPKASFDWLCAAAARGHAHAMSEVG
                     LRYSNGAGVERDFVKAAQWFAQAREAGEVSAGTLLAECYLLGKGVTKNEGKAIDLLQD
                     AATAKDARAMDQLATCYHKGIGVGRDDHQSFRLYSAAAKLNYLDSVGNLGVL"
     gene            1958..2860
                     /locus_tag="VNW28_06240"
     CDS             1958..2860
                     /locus_tag="VNW28_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXA09563.1"
                     /translation="MPAKLRITPLAGEPFELTIGNTATIGRTSDNTVCLSGSPLVSRQ
                     HALLRCHDGYQYQIIDLGSRNGTLVNDQRVVMPMTLAPGARILIARNELVFEQIEDDF
                     SNAHLEVTLAGSMAGVASAIRPVALLVCDIRGFSSMAEKISSSHLAQQLGVWFREAGN
                     LVTKSGGTIDKFIGDALLAYWSEGEEGIDCAATLRIARDLLTLAGTIKWANGEAFQVG
                     IALHFGSVTCGNVGLDAQRDATIIGDVVNTVFRLESVMKELNQAVLLSADFGDRLPPG
                     EKLVDLGERKLKGKQQAVRIYGLG"
     gene            complement(2845..3447)
                     /locus_tag="VNW28_06245"
     CDS             complement(2845..3447)
                     /locus_tag="VNW28_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXA09564.1"
                     /translation="MKIGAVVLAAGGSARLGRPKQLLRYGGQSLVRRAARAALEAGCA
                     PVAVVVGPDRDKIAPELRDLAVIILPNESWRRGMGTSIRAGVEALKDCDGLLILGCDQ
                     PRVDSTLIRQLVARQEETQKPMVASAYAGTRGVPAVFGRSCFAELLSLGDKQGAKVLL
                     VARPHDVATVDFPGGAMDIDTPGDWERLASHPDLAAQPRP"
     gene            complement(3444..4349)
                     /locus_tag="VNW28_06250"
     CDS             complement(3444..4349)
                     /locus_tag="VNW28_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HXA09565.1"
                     /translation="MKEIHDILAEFERHRGEPFALATLVRARGSSYRRPGARMLIASG
                     GGTVGALSGGCLEDEVALRAREVMTTGVPILLSFDTRLRYGCDGTIDVFVERVRPELL
                     DQLAAAQGARRACRIETIFEGNTSLGSRIVSSPDDAPAGAFLQTIRPPLQLLLIGAGP
                     DSAPLRGLAEILGWQLFEAEEASSLPNESDEWTAAVVKTHNYGRDYAALEKLLPLGLP
                     YLALLGPRRRRDQLLHTLLEAGVIARSELFSPAGLDLGAESPEEIALAVVAEIQATFA
                     AAPGASLRDRRIPIHRAIECAAVLT"
     gene            complement(4552..4622)
                     /locus_tag="VNW28_06255"
     tRNA            complement(4552..4622)
                     /locus_tag="VNW28_06255"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4588..4590),aa:Gly,seq:ccc)
     gene            complement(4675..5259)
                     /locus_tag="VNW28_06260"
     CDS             complement(4675..5259)
                     /locus_tag="VNW28_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXA09566.1"
                     /translation="MRQNPKVRNGFTLIELLVVITVIAILTALLFPAFKGVQDQAKRT
                     QARNDVTQIVTAVNAFYTEYGTYPLAAGTADVTVGGSGDANNALFTILRGLNATVNPR
                     NIIFISPPDVKDPASPRSGIGRDGQYFDPWGKPYAVAIDGNYDGQINNPYGASGGAGS
                     SPIRQGVIAWSLGKDGSLGLSGNFTGSDDVISWQ"
BASE COUNT          996 a         1671 c         1652 g         1019 t
ORIGIN      
        1 taaagcactc cgaggtttcc gaccgaatcg agatagttca acttcgcggc ggcgctgtag
       61 agtcggaaag actggtggtc atcgcgcccg accccgatcc ctttgtgata acaagttgcc
      121 agttgatcca tcgcgcgtgc atccttggcc gtggccgcat cctgcaggag atcgatcgct
      181 ttcccttcgt tcttggtcac gcctttgccg agcagataac attcggcaag caaggtgccc
      241 gcgctcactt ccccggcttc ccgcgcctgc gcgaaccatt gcgcggcctt gacgaagtcg
      301 cgctccacgc cggcgccgtt ggaataacgc aaacccactt ccgacatggc atgcgcatgc
      361 ccccgcgccg ccgccgcgca aagccagtca aaggaagctt tcgggtcgcg cttgcgcaga
      421 aaatcgccca gaatttccat cgcgctcacg acgttgagct tcgccgcggc ggtcagcggc
      481 gcgcgcaatt gttcgaaatt gtcttcgcgc agcgcgcctt tttcggactg caatctgctg
      541 agcaacaatt cgagacgcac ccgccccact tcgctctcgg gaaaatcttt ctgcaattgc
      601 acgtaggcca tgatggctcg gggccaatcc tgggccgctt cgtatttctc ggcggcggcc
      661 agcgaggtcc gcgatagctc ctgcccgctc aacgaagccg cgggtgacac cgaccgagcc
      721 gacggggcgg cggtcgcctg actgatcgcg ggcgtcgcgg aagcgaccgg tgacggcgct
      781 gccgaactgc tcgcctgcgt cggcgggaga attttctccg ttttgcgcgt cgtgagaaaa
      841 atcagcgcgc cggccccggt aagaacaact gcggccagcg cccaaatcat catgcgcgaa
      901 gacttgcgga cgggggcggg cggcacctcc acgggcaagg gagggggcgg cgcctccacg
      961 accgtcggaa aatcctgctc gcctctttgc ggggcggcct ggatatccgc gatgcatttt
     1021 tccaatgcgg cgcggagcgc ggccggcgtt tgcggccgcc gggccgggtc cttctcgatc
     1081 atattgccga cgagggcgcg cacgctcgcc gggagatttt gcagtttttc gagcggcggc
     1141 ggtttgtgca gatgctggct catgacctgc gcgagcgaac cgccgaaagg cgcctccccg
     1201 acgagcatgt accaaagcgt cacgcctaac gagtagatgt ccgagcgaac atcgatctct
     1261 ttttcctcga gctgctccgg gctggcgaaa tgcgcggtgc cgacaaaccc gccgagcgag
     1321 agcgttgcga ggtcgtcgtt cccttcatgc ttgattgatt tggcgaggcc gaagtcgatc
     1381 accttgacga gaaaatcgtc gtcctccctg acgagcatca ggttcgccgg tttgatgtcg
     1441 cgatgcacga gatgatgctt ttccgccgcg gcgagcgcgc gcgccacctg cagagtgatg
     1501 cggagcgcga tcacgggatc gatcggtccc tggcgcttga tgaaggattc gaccgtctcg
     1561 ccatcgacaa actccatcgc gtaaaagaac gcttcgctgc tcgtcccgag atgaaagacg
     1621 gaggcgacat tcggatgacg cagttgggcc gcggcgcggg cctcgcgcag gaaacgctgc
     1681 tgcgcgattt cgctctcgag aaatttcgcg ttgatcacct tgagcgcgac gttcacccgc
     1741 aggttggtat cgaacgcctt gtaggtgatg cccatcgcgc cccggccgag ctcgaaaagc
     1801 gaaccgtcct cgcgagtcag cacctcataa tgatcgaaag caattttctc ggccatcggg
     1861 aaagaccgga ttatatcgcc cgattcttcg agggcgctat cggataaaga cgatcttgtt
     1921 gcacgcgatc tgctgccaga tactgcccgc tgctgccatg cccgccaaac ttcggatcac
     1981 gccgctcgct ggtgagccgt tcgaacttac gatcggcaac accgccacca tcgggcggac
     2041 gagcgacaac acggtctgcc tgagcggcag tccgctcgtc tcacgccagc acgcgctcct
     2101 tcgctgtcac gatggttatc aatatcagat tatcgatctt ggcagccgca acggcacgct
     2161 ggtgaacgac cagcgcgtcg tgatgccgat gacactcgcc cccggtgcgc gcatcttgat
     2221 cgcgcgaaac gaactcgttt tcgagcaaat cgaagacgat ttttccaacg cccatctcga
     2281 ggtcacactg gccggctcga tggccggcgt cgcctccgcc atccggccgg tggcattgtt
     2341 agtctgcgac attcgcggct tcagcagcat ggcggaaaag atttccagct cccatctcgc
     2401 gcagcaactc ggcgtctggt ttcgcgaagc cggcaacctc gtgacgaaga gtggtggaac
     2461 gatcgataaa ttcatcggcg acgcgctcct tgcctactgg agcgaagggg aggaaggaat
     2521 cgattgtgcg gcaacgctcc ggatcgcgcg cgatctcctt accctcgcgg gcacaatcaa
     2581 gtgggccaac ggcgaggcgt ttcaggtcgg gatcgcgctt cattttggct cggtcacctg
     2641 cggcaacgtc gggctcgacg cgcagcgcga cgcgaccatc atcggcgacg tggtcaacac
     2701 cgttttccgc ctcgaatcgg tgatgaagga actgaatcag gcggtgctgc tctcggccga
     2761 tttcggcgat cgtctgccgc cgggtgaaaa attggttgat ttgggcgagc gaaaattgaa
     2821 gggcaaacaa caagccgtcc gcatctacgg cctcggttga gccgcgaggt ccggatggct
     2881 tgcgagtcgc tcccaatctc cgggtgtgtc gatatccatg gcgcctccgg ggaaatcgac
     2941 cgtcgcgaca tcgtgaggcc gagcgaccag cagaactttg gcgccctgtt tgtcgcctaa
     3001 cgagagcagc tccgcgaaac acgatcgccc aaaaaccgcc ggcacgcccc gcgtgccggc
     3061 ataggcggag gcaaccatcg gtttctgcgt ttcctcctgc cgcgcgacga gctgacgaat
     3121 cagggttgaa tccacccggg gctggtcgca gccgaggatc aggagtccgt cgcagtcttt
     3181 tagtgcctcc acgccggccc gaatcgacgt gcccattccg cgtcgccacg attcgttagg
     3241 caaaatgatg acggccaggt cgcgcaactc aggggcgatt ttgtcgcggt cgggtccgac
     3301 gacgaccgcg accggcgcgc aacccgcctc gagcgccgcc cgggcggcgc gcctaacgag
     3361 actctggccg ccgtagcgca agagttgctt cggtcgcccg agccgggccg acccgccggc
     3421 ggcgaggacg acggccccga tcttcacgtc agcaccgcgg cacactcgat cgcgcgatga
     3481 atcggaattc ttcggtcgcg caacgacgcg ccgggcgccg ccgcgaaagt tgcctggatt
     3541 tccgccacga cggcgagagc gatttcttcg ggggactccg cgccgaggtc gagtccggcg
     3601 ggagaaaaca attccgagcg cgcgatcacg ccggcctcga gcagggtgtg caggagttga
     3661 tcgcgccggc ggcgcggacc gaggagcgcg aggtaaggca gcccgagcgg cagcagcttc
     3721 tcgagcgcgg cgtagtcgcg cccgtagttg tgcgtcttca cgaccgccgc cgtccattcg
     3781 tcggattcgt taggcaaaga agaagcctcc tcggcttcga acagctgcca gcccagaatt
     3841 tccgccagcc cgcggagcgg tgcgctgtcc ggccctgcgc cgatgaggag gagttgtaac
     3901 gggggccgga tcgtctgcag aaacgcgcca gccggtgcgt cgtccggcga ggacacgatc
     3961 ctgctgccta acgaggtatt gccttcgaaa atggtctcga ttctgcaggc gcgccgcgcg
     4021 ccttgcgcgg cggccagctg atcgagcaat tccggtcgca cccgctcgac aaaaacgtcg
     4081 atcgttccgt cgcagccgta acgcaaccgg gtgtcgaagc tgaggaggat ggggacgccg
     4141 gttgtcatca cctcccgcgc gcgaagcgcc acttcgtcct cgagacagcc cccgctcaac
     4201 gctccaaccg tgccgccgcc cgaggcgatc agcatccgcg cgcccggccg gcgataactg
     4261 gaaccgcgtg ccctaacgag tgtggcgaga gcgaacggtt cgccgcgatg ccgctcgaat
     4321 tcagcgagga tgtcgtggat ttccttcatc tcttgtcatg cgagcgcagt cgcgggatcc
     4381 tgggaggcgc caaaggcaaa ggagctgcgt gtttccccgc gggatctttc gactccactg
     4441 cgctccgctc aggatgacac ggctcgagtt cctcggacat cgtcttttct ccgcaccgag
     4501 ggtcgtgtct ggttccttgg atttggaatg ccaacgcgtt tcgaaggaat gagcgggtga
     4561 ggggaatcga acccccgtat gcagcttggg aagctgccgt tctaccattg aactacaccc
     4621 gcgaaaggat tttcaaattt cgatcgggat tggcgattgg caagccgcgg aaatttactg
     4681 ccaggaaata acgtcgtcgg agccggtaaa gttgccgctc agcccaaggc ttccgtcctt
     4741 ccccagcgac catgcgatca cgccttggcg gatcggactg gagccagcgc ccccgctggc
     4801 gccataagga ttgttaattt ggccgtcgta gtttccgtcg atcgcgacgg cgtaaggctt
     4861 tccccaagga tcaaagtatt ggccgtcgcg accgattccc gatcggggac tagccgggtc
     4921 tttgacgtca ggaggcgaga tgaagatgat gtttcgcgga ttaacggtcg cattcaggcc
     4981 gcgcaggatt gtgaagagag cattattggc atcgccgctt ccgccgaccg tcacatctgc
     5041 tgtgcccgca gcgagagggt aggtgccgta ttccgtataa aaggcattca ccgcggtcac
     5101 gatttgcgtc acgtcattcc tggcctgcgt tcgtttcgcc tggtcctgca cgcctttgaa
     5161 agcggggaaa agcagcgccg tcaggatggc gatgaccgtg atgaccacga gcagctcgat
     5221 caaagtgaag ccgttccgaa ctttcggatt ttgcctcacg tccatttttg catctgctcg
     5281 gggaagggac attgcgcgca gtcggagtcg tcttgcaagc aaaagtcttc gtagatct
//
LOCUS       DATMNS010000183         4363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2391636, whole genome shotgun sequence.
ACCESSION   DATMNS010000183 DATMNS010000000
VERSION     DATMNS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4363
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2391636"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..464)
                     /locus_tag="VNW28_06265"
     CDS             complement(117..464)
                     /locus_tag="VNW28_06265"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXA09567.1"
                     /translation="MKSLSHSLAGAFFLGAILFVATSAPAQTAPVALVEGYVYRSTGQ
                     PVPGVTVSLVHPSVGRSSPAMTNPGGYYYFSNVPMRGDPYYIEVYWGTELLYRNTFFA
                     NAPRVSLPTIVLP"
     gene            complement(464..1075)
                     /locus_tag="VNW28_06270"
     CDS             complement(464..1075)
                     /locus_tag="VNW28_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09568.1"
                     /translation="MNSGDGFILAMTTLDWTVVSSAVCGLVMVVGGICLLYKGAITLQ
                     QAGPDEAVSLQFQKLLRIQTRYPALGLFIIGLAFILVGMFYSPKINPVTLQGKVDGAD
                     PSGVTIHVTSPEWSTVAPDSDGNFNLIVYPDVRRVEVVVQAAGCKPESRRFTLDASKS
                     KGGVLALPKMAFEAVAAKPAVGTIVAPPPNAALPPLDAKTTFN"
     gene            1147..2499
                     /gene="rmuC"
                     /locus_tag="VNW28_06275"
     CDS             1147..2499
                     /gene="rmuC"
                     /locus_tag="VNW28_06275"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXA09569.1"
                     /translation="MVSQQGVSPLLYLLLGLTGGGLLATLGFLLVRRGPQIDPILSGK
                     IEAFREAQERDERSREEEAARLREETSRAAKAQREELSTAMRTLGETNLKSIAEIGAI
                     LKSQLETVATQTGKLTQSNEARLESLRSVVDLRLKQLQEDNAQQIEKMRATVDEKLQG
                     TLEKRLGESFQMVSERLERVHQGLGAMQQLASDVGGLQRVLTNVKTRGGWGEVQLGAL
                     LEEVLAPEQFARNVRTREGSGENVEFAIRLPGNENGVPVWLPIDAKFPVEDHQRLIAA
                     QESADPIATEQAMKNLETQLRKSAKDICAKYINPPQTTNFALMFLPTEGLFAEAIRRI
                     GLVEQVRRESKVIFAGPTTLAALLNSLQMGFRTLAIQKSSSEVWSLLATVRTEFGKFG
                     GLLDGVKKKLEQASNQIEDMARKSRTIEKRLNRVEELPSHPEPLLPDLLPNEETEGEE
                     "
     gene            complement(2536..4311)
                     /locus_tag="VNW28_06280"
     CDS             complement(2536..4311)
                     /locus_tag="VNW28_06280"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXA09570.1"
                     /translation="MKLTGLRPLLVALFLGVATKIWAQTAETPSPDADFDKLALDFTG
                     GYLAARPLQGIALGFHQYDGKIGDYSQLAIDAEVERLKRFQDQLAKIDPSKLSKGAAI
                     DRRILQTAIAGELFKIVDMDVFDKNPMTYAGALNVNIYIKRDFKPLEDRVRDIVTIED
                     QAANIMTAAKTNLLPVLPKPYVELAIQIANGSADFLEKDLTAALKDVKDESLMAAFQD
                     SNKKAAAALRDFASWLTRERLPKATADFALGAKKYQQMLAGTGLVDLPPDKILAIGLQ
                     RLKEEQDIFVAAAKIIDPGKKPIDVFKQIQSEHPQPGELVTDVAKDLDQIRQWVVDHH
                     IVAIPSDVRATVLATPQFARATTFASMDTPGPFEKKATQAYYYITPVEDNWTEQQKNE
                     WLTSFNFYTTDIVSIHEAYPGHYVQFLHLNASPATQVEKIFGNYAFIEGWAHYCEKMM
                     LDEGFGSAPNPTPEQKVRAAKYRMAQADEALLRLCRLCVSIKMHTQGMSVADAAKFFH
                     ENCYYEEKPSMSEAMRGTFDPGYLNYTLGKLQILKLRDDYKAQEGANFSLEKFHNELL
                     THGAPPIRLLREIMLKDKSKWGALL"
BASE COUNT          903 a         1238 c         1364 g          858 t
ORIGIN      
        1 agataaccta acgaacgagc attacgcgga ggtatttggt tttccggctt tgggtcgggc
       61 ggcgtacgga gggttccgcg tatccttcta gcggtggatg ggcgctcgcc gcccgctcag
      121 ggaagaacga ttgtcggaag cgatactcgc ggagcgttcg cgaaaaaagt gtttcggtag
      181 agcaactccg tcccccaata gacttcaata taataggggt ccccccgcat cggcacattg
      241 gaaaaatagt aataacctcc cgggttggtc atcgcaggcg agctgcgtcc gacggagggg
      301 tggacgagtg agacggtgac gcccggcacc ggttggcccg tcgaccgata gacgtaacct
      361 tcgacgagcg cgacgggggc agtttgcgcg ggcgcggaag tggcgacgaa caaaatcgcg
      421 ccgagaaaaa atgcaccagc gagggaatga gaaagcgatt tcattagtta aaagtcgtct
      481 ttgcgtcgag cggcggcagc gcagcattcg gcggcggagc cacgatggtg ccgacagccg
      541 gcttggctgc taccgcctcg aaagccatct ttgggagcgc caagacgccg cccttacttt
      601 tcgaagcatc gagtgtgaaa cgcctcgatt ccggtttgca gcctgccgcc tgcacgacga
      661 cctccactcg ccgcacgtcg ggataaacga tcaggttgaa gttcccgtcg gagtcgggcg
      721 caactgtgct ccactcgggc gacgtcacgt ggattgtcac acccgacgga tcggcgccgt
      781 ccactttacc ctgtagcgtg acgggattaa ttttgggcga ataaaacatc cccaccagaa
      841 tgaaagccag cccgatgatg aacagcccca gtgcgggata acgcgtttga attctgagca
      901 gcttctgaaa ctggagcgag acggcttcat ccggcccggc ctgctggagt gtgatcgccc
      961 ctttgtaaag caggcagatt ccgcctacga ccatcacaag gccgcaaacg gcgctgctta
     1021 caaccgtcca atcgagcgtc gtcatggcga gaatgaatcc atcgccagag ttcatagcgg
     1081 attcggcctc cggctggcaa tattatttct tgcggtcatg atcgttgctt gaagcgcgcg
     1141 gcgaccgtgg ttagtcaaca gggggtgagt ccgctgcttt atcttcttct cggcctaacg
     1201 ggcggcggac tgctcgccac tctcgggttt ctgctcgtgc ggcgcggacc gcagatcgat
     1261 ccgattttgt caggcaagat cgaggcgttt cgcgaggcgc aggagcgcga cgagcggtcg
     1321 cgggaagagg aggccgcgcg tctgcgcgaa gaaacatcgc gggcggcgaa agcgcagcgc
     1381 gaggagttaa gcacggccat gagaactctc ggcgagacga atctcaaatc gatcgcggag
     1441 atcggcgcga ttttgaaaag ccagcttgaa accgtcgcaa cacagactgg caagctgacc
     1501 caatcaaacg aagcacgtct cgagagtttg cgctcggtgg tcgacctgcg tttgaagcag
     1561 ctgcaggaag acaacgcgca acagatcgag aaaatgcgcg cgacggtcga cgagaaactg
     1621 caagggacgc tggagaagcg gcttggcgaa tcgtttcaga tggtgagcga acgcctcgag
     1681 cgcgtgcacc aggggctcgg cgcgatgcag cagctggcga gcgacgtcgg agggttgcag
     1741 cgcgtcctga cgaatgtgaa aacgcgcggc ggctggggcg aagtccaact cggggcgttg
     1801 ctcgaggagg tgctggctcc ggaacaattc gcccgcaacg tccggacacg cgaaggcagc
     1861 ggcgagaacg tcgagttcgc gatccgttta cccgggaacg aaaacggtgt gccggtctgg
     1921 ctgccgatcg acgcgaaatt tccggtggag gatcaccagc gcctgatcgc ggcacaggaa
     1981 agcgccgatc cgatcgcgac cgaacaggcg atgaaaaatc tcgagacgca attgcggaaa
     2041 agcgcgaaag acatttgcgc gaaatacatc aacccgccgc agacgacgaa cttcgccctc
     2101 atgtttctgc cgaccgaggg actctttgcg gaagcgatcc ggcggatcgg actggtcgag
     2161 caggtgcggc gggaaagcaa agtgatcttc gccggaccga ccacactggc ggcgttgctc
     2221 aacagcctgc agatgggatt ccgcacgctc gcgatccaga agagctccag cgaagtctgg
     2281 agcctgctgg ccacggtgcg gaccgagttc ggaaaatttg gcggcctgct cgatggcgtg
     2341 aagaagaaac tggaacaagc ctcgaaccag atcgaggaca tggcgcgcaa gtcgcgcacg
     2401 atcgaaaagc gcctaaaccg ggtcgaggag ttgccctcgc atccggagcc gctgctgccg
     2461 gacttgttgc ctaacgagga gacggaaggg gaggaatagg cgggccgtca cgccgcgttc
     2521 cgcaccgcga aacggctaca ggagcgcgcc ccactttgac ttgtccttga gcatgatctc
     2581 gcgcagcagc cgaatcggcg gcgcgccgtg ggtcaacagc tcgttgtgga acttctcgag
     2641 cgaaaaattc gcgccttcct gcgctttgta atcgtcgcgc agcttgagaa tctggagctt
     2701 gcctaacgtg tagttcaggt agcccgggtc aaacgttccg cgcatcgctt cggacatcga
     2761 cggtttctct tcgtagtagc agttctcatg gaaaaacttc gccgcatcgg cgaccgacat
     2821 tccctgggtg tgcatcttga ttgagacgca gagccggcag agtcgaagca gcgcctcgtc
     2881 cgcctgcgcc atgcgatact tcgcggcgcg caccttttgt tccggagtcg ggttcggcgc
     2941 gctcccgaag ccttcgtcga gcatcatttt ctcgcagtaa tgcgcccagc cttcgataaa
     3001 agcgtagttc ccgaaaatct tttcgacctg ggtcgccggc gaagcattca ggtgcaggaa
     3061 ctgcacgtaa tgcccgggat aggcctcgtg aatggagacg atgtcggtgg tgtaaaagtt
     3121 aaatgaggtc agccattcgt tcttctgctg ctcggtccaa ttatcctcaa ccggcgtgat
     3181 gtagtaatag gcctgcgtcg cttttttctc gaacggcccc ggtgtgtcca tcgaggcgaa
     3241 ggtcgttgcg cgagcgaact gcggcgtcgc cagcacagtc gcgcggacat cggacgggat
     3301 cgcgacaatg tgatggtcga ctacccattg ccgaatttgg tcgaggtctt tcgccacgtc
     3361 ggtcaccaac tcgcccggtt gcggatgttc gctttggatc tgcttgaaga cgtcgatcgg
     3421 ttttttcccc ggatcgatga tcttggcggc ggcgacgaaa atatcctgct cctctttcaa
     3481 gcgctgcagt ccgatggcaa ggattttgtc tggcggcagg tcgaccagcc ccgtgccggc
     3541 cagcatctgc tgatattttt tcgcacccaa agcgaagtct gcggtcgctt tcggcagccg
     3601 ctctctcgtc agccagctcg cgaaatcgcg cagcgcggcg gccgccttct tgttcgaatc
     3661 ctggaacgcc gccatgagcg attcgtcttt cacgtccttg agcgctgccg tcagatcctt
     3721 ctccaggaaa tcagccgagc cattcgcaat ctggatcgca agctcgacat agggtttggg
     3781 caggaccggc agcagattcg tcttggcggc ggtcatgatg ttggctgcct ggtcctcgat
     3841 cgtgacgatg tcgcgcaccc ggtcctccaa aggcttgaaa tcccgcttga tgtagatgtt
     3901 gacgttgagc gcgcccgcgt aagtcattgg gttcttgtcg aaaacgtcca tgtcgacgat
     3961 cttgaaaagc tccccggcaa tcgcggtttg cagaatccgc cggtcgatcg cggcgccctt
     4021 actcagcttg ctcgggtcga ttttggccaa ctgatcttgg aagcgtttca ggcgttctac
     4081 ttccgcgtcg atcgcgagct ggctgtagtc gccgatcttg ccgtcatatt ggtggaaacc
     4141 gagcgcgatg ccctgaagcg gccgcgcggc caggtagcca ccggtgaagt cgagcgcgag
     4201 cttgtcgaaa tcagcgtccg gcgacggcgt ctcggcggtt tgcgcccaaa tcttggtggc
     4261 aacgcccagg aaaagcgcga cgagaagcgg gcggaggccg gtcaatttca cgggcgcagt
     4321 ttaaaagcgg atggcagcct tgtcatgcct ccccattggc aaa
//
LOCUS       DATMNS010000184         6070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3707423, whole genome shotgun sequence.
ACCESSION   DATMNS010000184 DATMNS010000000
VERSION     DATMNS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6070
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3707423"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1053)
                     /locus_tag="VNW28_06285"
     CDS             complement(34..1053)
                     /locus_tag="VNW28_06285"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXA09571.1"
                     /translation="MKKKLKVLALFDAIGPTTIDQDLSAELKTEDWKTEANVLAALRA
                     LGYSTEHLAIFDDLDLLQQKLATFAPDALFNLADQFKNNRGFDQNIVSFLEMQGVPFT
                     GCGATGLVLCKHKGISKKILSYHRIHTPNFVVIPRGQRIARPRQLKFPLLVKPVKEEA
                     SYGIAQASFVETDEQFRERVSFIHEKHDGDVIAEEYIAGRELYVSLMGNLRLTVFPIR
                     ELVFREVPPDEPKIATYRAKWDEEYRKRWGLQNQFAEGLEPALVAQIEQTCKRIYRLL
                     TIDGYARIDLRLTAANEVYFIEANPNPILAEDEDFAQSAGKAGLAYPQLIDRIIRHGM
                     KTLRS"
     gene            complement(1056..2072)
                     /locus_tag="VNW28_06290"
     CDS             complement(1056..2072)
                     /locus_tag="VNW28_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09572.1"
                     /translation="MEAADWASLNDQELLERRISKLGLRLEGTALEPLIRQLYDELLA
                     RGLVLRPPCHIGDEWFVPVGIPAIFIPFFLVHERLRALEKTMMLEVEGGTAEWFMKLM
                     RHEAAHAYSYAYHLPRKKKWRLTFGRTSRDETPDFYQPRPFSRSYVVNLDDWYAQSHP
                     DEDFAETFAVWLTPGLDWRKRYTGWKALQKLEYVDELMRSLAGNPPLHLPDYRVADYD
                     CLNLKLKTYYARKRKLYEDTYPDFYDADLRLLFAAAAGPGRITASAYLRRRRRRLLNS
                     VCQWTKERKFRVNKLLARLIDRCDQLGLHVLNDDPEQDFRVTSFITTLVMNYLFTGKF
                     KRTK"
     gene            2290..2592
                     /locus_tag="VNW28_06295"
     CDS             2290..2592
                     /locus_tag="VNW28_06295"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HXA09573.1"
                     /translation="MKPLDVFRHGQHAVHVKSITYTAARENLASTINRVCEDHAPVVI
                     TRNRDQAVVMLSLAEYESLQETAYLLRSPKNARRLLAAVDTLQRGRGIKRKLKLDV"
     gene            2589..2846
                     /locus_tag="VNW28_06300"
     CDS             2589..2846
                     /locus_tag="VNW28_06300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HXA09574.1"
                     /translation="MNLVFAPQAWDDYLYWQKTDPQTISRINLLIKEIARDPCGGIGK
                     PEPLKHALQGYWSRRITSEHRIVYKKARDQILIAQLRYHYR"
     gene            complement(2886..3419)
                     /locus_tag="VNW28_06305"
     CDS             complement(2886..3419)
                     /locus_tag="VNW28_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09575.1"
                     /translation="MVRDIGVEICALAQVAVRFGEFKIRTGSGRSAGAKMRGLRNRHM
                     LKITMPKYSTAKTLRASSSTRKSARVKEPEEGAAAAELALKAHVLRSDPEILADMPEG
                     DRVMQEIVDMVNLTEAPEDAVASLVIDLLQYCEREKIDWTQEVMFRARKHLRSEPIDE
                     VQGDEFPEAERDRIAAS"
     gene            3498..5918
                     /locus_tag="VNW28_06310"
     CDS             3498..5918
                     /locus_tag="VNW28_06310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09576.1"
                     /translation="MFTDLRFALRQLFKSPGFTLLAVITLTLGIGLNTAIFSLINDLF
                     LRGLPFQDPDRLVDFYSGTKTAQALEWPLSLPRFQLYRQGQTLFDGFAADNGVAVTLT
                     GLGDPVQIGAFRVTANYFDVLGVRPMRGRTFLPDEEEGADVAIVSEHFWQSRLGGDPN
                     VLGRSITLDGLAHTIVGVIPNMPVPWVGPNANEVWTTKPFVIPGFSHERMMRGTAFLR
                     TVGRLKPGLTIEQARAALPALEQSYRAQNPGKIDADLKTRIAALPEDATGKLRPAFAT
                     LFAAVSFVLLIACSNVANLLLVRFSGRRREIALRMALGASRTSVLRLFILESLLVSAL
                     AGSLGALLAWQLIPLVPKITTNFLPLETGGVSISLPVLGFTVALSLLTGLAMGFYPAW
                     QSSRADLVDGLKEGGRGASGSLRQQRFRRILVGAQVALSVALLASAALLIASFVRLSR
                     QPLGFRPDRLWVAFLTFPQTRYPDEASRARFADRVQEALRVIPGFESATASGDFPLAG
                     GNGATLYTRPEGNVLPVPERASAPSHDVMPGYFHTWGIPILAGRDFNAHDLAGQPNVM
                     LISQAGARKVFGHENPVGKTLLVTSASTPAEIVGVVGDVRSGKIGQPNEMEFYRPWAQ
                     ENFPFMSITVQSPMKTAAVTHLVQRALTQIDPTMALALPQSMDEVVRQALGEARLMML
                     LLGIFAGVALLLATVGIYGAVAYTVAQRTGEIGVRMALGAQTLDVVRLVVGQGMKPVF
                     FGLIIGLAAALALGRLIAAQLYEVSAHSPFLLGGTLAILGLAALLACLFPARRASRLN
                     PVIALRTE"
BASE COUNT         1135 a         1849 c         1779 g         1307 t
ORIGIN      
        1 gccccgcatc cgtgcctaat gagaagtcac caatcagctc cgcagcgtct tcatcccatg
       61 ccggatgatc cggtcgatca gttggggata agccagacca gccttgcccg ctgattgcgc
      121 aaagtcttcg tcctccgcga ggatgggatt ggggttcgcc tcgatgaaat aaacctcatt
      181 cgccgccgtc agccgcaaat cgatacgcgc gtaaccgtcg atcgtgagca gacggtaaat
      241 gcgcttacag gtctgctcga tctgcgcgac gagcgccggc tcgagtccct cggcgaactg
      301 attctgcaaa ccccagcgtt tgcgatattc ctcatcccac ttcgcccggt aggtggcgat
      361 tttcggctcg tccggcggca cttcacggaa gaccagctcg cggatcggga agacggtcag
      421 ccgcaggttg cccatcaggc tgacgtagag ttcacggccg gcgatgtatt cctcggcgat
      481 cacgtcaccg tcgtgtttct cgtgaataaa agaaacacgc tcccggaact gctcgtcggt
      541 ctcgacgaag gaggcctgcg cgatgccgta ggacgcctct tctttcaccg gcttgaccag
      601 gagtggaaac ttaagctgcc ggggtcgtgc aatccgctgg ccgcgcggaa tgacgacgaa
      661 gttcggcgtg tgaatgcgat ggtaactcag gatctttttc gagatgccct tgtgtttgca
      721 aagcaccaga ccggtcgcac cgcagccggt aaagggcacg ccttgcatct cgagaaagga
      781 aacgatgttc tggtcgaagc cgcggttgtt cttgaactgg tcggcgaggt taaaaagagc
      841 atcgggcgca aaggtcgcca gtttttgctg caagagatcg aggtcgtcga aaatggcaag
      901 atgctctgtc gagtagccga gcgcgcggag cgcggccagg acgttcgctt cggttttcca
      961 atcctccgtt ttcaactccg cgctcaggtc ctggtcgatc gttgtcggac cgatggcgtc
     1021 gaaaagggcg aggactttaa gtttcttttt caccgttact tggtgcgctt gaacttgccg
     1081 gtgaaaagat aattcatcac cagcgtcgtg atgaaggagg tgacgcgaaa atcctgctcc
     1141 ggatcgtcgt tcagcacatg gagcccgagc tggtcacagc gatcgatcag tcgggcgagg
     1201 agtttgttca cccggaattt tctctccttc gtccattggc agaccgagtt caacagtcgt
     1261 cgtcgacgcc ggcgcagata cgcgctggcc gtaatccgtc ccggtccggc ggccgcggcg
     1321 aagagcagtc gcagatcggc gtcgtagaaa tccggatagg tatcttcgta gagtttgcgc
     1381 ttgcgcgcgt agtaggtctt gagcttgagg ttcagacaat cgtagtcagc cacgcgataa
     1441 tccgggagat gcagcggcgg attcccggcg agcgagcgca tgagttcatc gacgtattcg
     1501 agtttttgca gcgctttcca gcccgtgtaa cgcttgcgcc agtcgagccc cggcgtgagc
     1561 cagaccgcga aggtctcggc gaaatcttcg tccggatgac tctgcgcata ccagtcgtcg
     1621 agattcacca cgtaactgcg gctgaacggg cgcggctggt agaaatccgg cgtctcgtcg
     1681 cgtgacgtcc gcccgaaagt cagccgccat tttttcttgc gcggcaggtg gtaggcgtag
     1741 ctgtaggcgt gcgcggcctc gtggcgcatg agtttcatga accactcggc cgtcccgccc
     1801 tccacctcga gcatcattgt cttctcgagc gcgcgcagcc gctcgtgaac gagaaaaaac
     1861 gggatgaaga tcgccgggat gccgaccggc acgaaccatt catcgccgat gtgacagggc
     1921 ggacgcaaca ccagcccgcg cgccaaaagt tcgtcgtaaa gctggcggat gagcggctcg
     1981 agtgccgttc cttcaagtcg caaaccgagc ttggaaatgc ggcgttcgag caattcctga
     2041 tcgttcaatg atgcccaatc agccgcttcc atttcgcgca cgttatcagt ccgcgccggg
     2101 tgctgtcacg ccttcccgcc ggccggggag cgcactgcct gcccgccgtg gtgggcgact
     2161 cgtccagcgc ctgcgggagc gatgacgccc tcgccatcgc gaattttcaa aagacttcaa
     2221 tctgaatgaa aagggctaaa caaaaacgtt ctcaaaggca tggcatcgtg agcggctcat
     2281 gataaacgaa tgaaaccgct tgacgttttc cgacatggac agcatgctgt ccatgtgaag
     2341 tctattacct atacggcggc ccgagagaac ctcgcgagca cgatcaatcg agtttgcgag
     2401 gaccatgcgc cagtcgtgat tacaagaaat cgcgaccaag cggtggtgat gctttctctg
     2461 gcagagtatg agtcacttca ggaaacggcc tacctgctcc gctcaccgaa gaatgccagg
     2521 cggttgctgg ccgcggttga tactttgcaa agaggcagag gaattaaacg caagctcaag
     2581 ctcgacgtgt gaatctcgtt ttcgccccgc aagcgtggga cgattatctt tattggcaaa
     2641 agaccgatcc gcagacgatt tcgcgaatca accttctcat caaagaaatc gcgcgagatc
     2701 catgtggcgg gatcggcaaa cctgaaccgc tgaagcatgc gctccagggt tattggtcgc
     2761 ggcgaatcac ttccgagcac cgcatcgttt acaagaaggc tcgcgatcaa attctcatcg
     2821 cacagctgcg gtatcactat cgatgaattg gatgcgccgg agatccttca agctcctgca
     2881 atgccttaag aagcggcaat tcgatctcgc tctgcttcgg gaaactcatc accttgaact
     2941 tcgtcgatgg gttcactgcg gaggtgtttc cgcgcccgaa acattacctc ttgagtccag
     3001 tcaatttttt cccgctcgca atactgcaag aggtctatga caaggctggc gaccgcatct
     3061 tctggcgcct cggtcaggtt gaccatatcc actatttcct gcatcacccg gtctccctcc
     3121 ggcatgtcag cgagaatctc aggatcggat ctcaatacgt gagctttcag cgccagttcg
     3181 gcagccgcag ctccttcctc cggttctttg acgcgggcgc tttttcttgt gctcgaagac
     3241 gcgcgaaggg ttttcgcggt ggagtatttc ggcatggtga tctttaacat gtgacggttt
     3301 cgaaggccac gcattttggc tccggcactt cgtccagatc ccgtccggat tttgaactcg
     3361 ccgaaccgaa ccgctacctg cgctaaagca caaatttcga cgccaatgtc gcgaaccaca
     3421 gacaaaactc gacagcagcg gcgtctcttg tttgaatcca agcgcgcctg acggtgcatc
     3481 tcaaccttgc cagtcgtatg tttaccgacc ttcgttttgc tctgcgacag ctcttcaaat
     3541 cgcccggctt tacgctcctg gcggtcatca cgctcacgct cgggattgga ttgaacacgg
     3601 cgatctttag cctgatcaac gatctctttc tgcgcggtct tcctttccag gatccggacc
     3661 ggctcgttga tttttattcc gggacaaaaa ccgcccaggc cctggaatgg cccctttccc
     3721 tgccgcgctt tcaactctat cgccaggggc agacgctctt tgacggcttc gcggccgata
     3781 acggcgtggc cgtcacgctg accggcctgg gtgatccggt gcagatcggc gctttccggg
     3841 tgaccgcgaa ttatttcgac gtcctcgggg tgcggccgat gcgcgggcgg acttttttgc
     3901 ctgacgaaga ggaaggcgcc gatgtcgcca ttgtctcgga gcatttctgg cagagccgat
     3961 tgggcggcga tccgaacgtc ctcgggcgta gcatcacgct cgacggcctg gcgcacacca
     4021 tcgtgggcgt tatccccaac atgccggtgc cgtgggtcgg gccgaatgcc aacgaggtct
     4081 ggacgaccaa accgttcgtc attcccggct tctcccacga acgcatgatg cgcgggacgg
     4141 cttttcttcg cacggtggga agattgaaac ctggcctgac gatcgagcag gcccgggccg
     4201 ctttgcctgc cctggagcaa agttaccgcg cacagaaccc gggcaaaatc gatgccgacc
     4261 tgaagacgcg gattgcggct ctgccggaag acgcgaccgg caagttgcga ccggccttcg
     4321 ccaccctctt cgccgccgtc agctttgtcc tcctgatcgc ctgcagtaac gtcgccaatc
     4381 tgctcctcgt ccggttcagc gggcgacgcc gggagatcgc gctccgcatg gcgctcgggg
     4441 cttcacgcac cagcgtgttg cgtcttttca ttctcgaaag tctgcttgtc agcgcgctcg
     4501 cgggttcgtt aggtgcactc ctcgcctggc aactcattcc gctggtcccg aaaatcacga
     4561 ccaattttct cccgctcgaa accggcgggg tgagcatctc gctgcccgtg ctcggtttca
     4621 ccgttgctct ctcgctcctc accggcctcg cgatgggatt ctatccggcc tggcaaagct
     4681 cgcgcgctga cctggtcgat ggtctcaagg aaggaggtcg cggcgcgagc gggagcctgc
     4741 gccagcaacg tttccgcaga atcctggtcg gagcccaggt cgcgctttcc gtcgcgttgc
     4801 tcgccagcgc cgccttgctc atcgccagct tcgttaggct gagtcggcag ccgctcggtt
     4861 tccggcccga tcgtctctgg gtggcgtttc tcacctttcc gcagacgcgt tatcccgatg
     4921 aggcctcgcg cgcccgtttc gcggaccggg tgcaggaggc gttgcgcgtt atcccaggtt
     4981 tcgaaagcgc gacggcgagc ggagattttc cgctcgccgg cggcaacggc gccacgcttt
     5041 atacccggcc cgaaggcaac gtcctcccgg tcccggaacg cgcctccgct ccctcgcacg
     5101 atgttatgcc gggatacttt cacacctggg gaattccgat tctggccggc cgcgacttca
     5161 atgcccacga cctcgcaggg cagcccaatg tgatgctgat cagccaggcg ggcgcgcgca
     5221 aagttttcgg gcacgaaaac ccggtcggca aaaccttgct ggtgacgagc gcgagcacgc
     5281 cggcggaaat cgtcggcgtg gtgggcgacg tgcgttccgg gaaaatcggt cagcctaacg
     5341 aaatggaatt ttaccggccc tgggcgcagg aaaattttcc cttcatgagc atcacggtgc
     5401 agagcccgat gaagaccgcc gctgtcactc atctcgtgca acgggccttg acgcagatcg
     5461 acccgacgat ggcgctcgcc ctgccgcaat cgatggatga ggtcgtcagg caggcgctcg
     5521 gggaggcgcg cctcatgatg ctgttgctcg gcatttttgc gggcgtggcg ttgttgcttg
     5581 cgaccgtcgg gatctacggc gccgtcgcct acaccgtcgc acaacggacc ggcgagatcg
     5641 gcgtgcgcat ggccctcggc gcgcaaacgc tcgacgtcgt gcgtctcgtt gtcggccagg
     5701 gaatgaaacc ggttttcttt ggtttgatca tcggcctcgc cgcggctctc gcgcttggtc
     5761 gcctgatcgc ggcccagctt tacgaggtct cggcgcacag tccgttcctt ctcggaggaa
     5821 cgcttgcgat tcttggcctg gccgcgcttc tcgcctgtct cttccccgcc cgccgcgcga
     5881 gccggctcaa tcccgtgatc gcattgcgga cggagtgagt tcatgaacga ctcggcttcc
     5941 cgctcgggag ctgcaaccga gcctggagat ccggcgttcc aacagctcct ggtcgcttaa
     6001 cgatgcccga tcgattgctt taccggctta ctcgcgcgag cgctgcgcgc gtcaagggca
     6061 gataaggatc
//
LOCUS       DATMNS010000185         4278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_970718, whole genome shotgun sequence.
ACCESSION   DATMNS010000185 DATMNS010000000
VERSION     DATMNS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970718"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VNW28_06315"
     CDS             complement(<1..521)
                     /locus_tag="VNW28_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXA09577.1"
                     /translation="MLNRLWQAVNICSARRAFALLYQGHAQVVSADQQNNFATHDFAS
                     WLDVSLTAPEAEHEMVTTISWKIRVPRVIVLLLFDRLPKKEVKFTRHNVFERDNNTCQ
                     YCGKVYERSDLNLDHVLPRDKGGLTTWENVVCSCIPCNTRKGNRLPHEAHMTLIRKPK
                     RPKWRPFVHVTFSG"
     gene            759..4145
                     /locus_tag="VNW28_06320"
     CDS             759..4145
                     /locus_tag="VNW28_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXA09578.1"
                     /translation="MRIPSRLAASLLLLAVVLLPLRFAAGQKLPETTRLLRFPATNGE
                     QIVFCYAGELYTVPKEGGVARRLTSGPGYSSFPRFSADGKQLAFTSQYDGNTEVYVMP
                     GDGGVPKRLTTSATLGRDDISDRMGPNNLVMTWENTRPFVVFRSRMKSFDSFIGELNT
                     VGLDAELPQQLPVPRGGFVSFSPDDSKMAFNRIFREFRTWKHYRGGMADDVWIYDFKT
                     GATENLTNDPAQDICPMWGPDNHIYFVSDRDGRMNLFSIDLANKETKQLTTFKDFDIK
                     FPSIGKGAIVFEQAGYIWRYDLGSGQCAPVPIEIKEDLASGRSALVDASKHIESVSLA
                     PDGQRVIAVARADLFSVPAKDGTPRNLTKTSNAHERDAVWSPDGKWIAYNSDITGENE
                     LYVRAQDGKGQPQQVTKGADTYYYAMLWSPDSKKLLWSDRLQRLRYVDVASKAVTQVD
                     QDKYGEVGSYDWSPDSQWIAWSRPEDNGLSKVYLYSTASKKTVPVTDDWYSAGQSKFS
                     DDGKYLLFTSSRDFKPIFGEEDFADVYRDMERIYLVTLARETESPLAPRSDEVGKAEK
                     KGKDAEEKKPDDAAKKKDGKDEKKKPVVVKVDPDGLGNRIDAFSIAPGNYTDLRMVDD
                     RLFYLRRTVADDKDDDNEEGGGQDRNAHLCAYSLEDRKETVLGDVNSYEITADGKKML
                     VKIKKDYAIIDLPKDKLETKDHELKLAGLDMQLDRHAEWNQIYFEAWRQMRDFFYSPT
                     MNGVDWKAMRDKYAALLPFVNHRNDLTYLIGELIGELNNGHAYVAGGERPETPRIKLG
                     LLGAEFSRDAATRAYRIDRILPGENWEDDTKSPLTAIGLNVKVGDYILAINGTPVASL
                     PNIYDALIGTAGKQVILRINSQPNDQGARDITVVPIDDESPLYYLAWVRKNIDYVNRK
                     TGGQVGYVHIPDMGQPGLNQFTKLYFPQIRKKGLIVDVRGNGGGFVSPLVIDKLRRAL
                     VMVEMSRNGVGRTDPPNTFLGPMATLINEFSASDGDIFPFRFKTLGLGKLIGKRTWGG
                     VIGIRNPLPLADGGQLFKPEFAPYSKDGKGWVIESHGVDPDIVVDNDPAKEFRGEDQQ
                     LDRGIEEVQTEMKTKAYELPPIPPFPNRNPANGGSSSTNP"
BASE COUNT          920 a         1305 c         1211 g          842 t
ORIGIN      
        1 ccgctgaaag tgacgtggac aaacggccgc catttcggcc gcttcggctt gcgaatgagg
       61 gtcatgtgcg cctcatgcgg cagccggttg cccttgcgcg tgttgcacgg gatgcaggag
      121 cagaccacgt tttcccaggt cgttaggccg cctttgtcgc ggggcaggac gtgatcgaga
      181 ttcaggtcgc tgcgctcgta taccttcccg caatactggc aggtgttgtt gtcccgctcg
      241 aagacgttgt gccgggtgaa tttcacttcc ttcttcggga gtcgatcgaa gagcaggagc
      301 acaatcaccc gtggcacccg gatcttccac gagatcgtcg tgaccatctc gtgctcagcc
      361 tcgggcgctg taagagagac gtcgagccag ctcgcgaaat cgtgggtcgc aaaattattc
      421 tgctgatcgg ccgagacgac ctgggcgtgc ccctgataaa ggagcgcaaa agcacgacgc
      481 gccgagcaga tattcaccgc ctgccagagg cgattgagca ccaggacctg actgttaagt
      541 agggtgtgca gagtttgctt cgcgcggcct aaccaattcc gcctgctcga tgtggcgcga
      601 aattatcacc cggaaatttc gttgtcaacg taacgaactc gtgacgaccg ccgaggctcg
      661 ggccggcaat cgatcggctc tttcgcctcc gctcggatga aaggcggttg catccggccg
      721 attcaaacgc agctttctcg ttcttcattc atcacattat gcgcattcct tcacgcctcg
      781 ccgcttcact ccttctcctg gccgtcgttc tcctgccgct ccgtttcgcc gccggccaga
      841 agctcccgga aaccacgcgt ctcctgcggt ttcccgcgac caacggtgag cagattgtct
      901 tttgctatgc gggcgaactc tacaccgtcc cgaaagaggg cggcgtcgcc cgccgcctga
      961 cgagtggtcc cggctacagc tctttcccgc gcttctcagc cgatggcaag cagctcgcct
     1021 tcacttccca gtatgacggc aataccgagg tttacgtcat gcccggcgat ggcggcgtgc
     1081 cgaagcgcct caccaccagc gcgaccctcg gccgggacga tatttccgac cggatgggac
     1141 caaacaacct cgtcatgacc tgggaaaaca cgaggccgtt cgtcgttttt cgttcccgga
     1201 tgaagtcgtt cgattccttc atcggcgagc tcaacaccgt cggcctcgat gccgagttgc
     1261 cccagcaatt gcccgtgccg cgcggcggtt ttgtctcctt ctcaccggac gattcgaaga
     1321 tggcgttcaa ccgcatcttt cgcgaattcc gcacctggaa acattaccgc ggcggaatgg
     1381 cggacgacgt ctggatttac gacttcaaga ccggtgcgac cgagaatctg acgaacgatc
     1441 cagcccagga tatctgcccg atgtggggtc ccgataatca catttatttc gtctcggatc
     1501 gcgacggccg aatgaatctt ttctcgatcg acctcgcgaa caaagaaacg aagcagctga
     1561 ccaccttcaa ggatttcgac atcaagttcc cgtcgatcgg caaaggcgca atcgtcttcg
     1621 aacaggccgg ctacatctgg cgctacgatc tgggcagcgg gcagtgcgcg ccggtgccga
     1681 ttgagatcaa ggaagacctc gcctccgggc gctcagccct ggttgacgcg tcgaagcaca
     1741 tcgaatccgt cagcctcgcg ccggatggcc agcgcgtgat cgcggttgcc cgcgccgatc
     1801 tcttttccgt cccggccaag gacggcaccc cgcgcaatct gacgaaaacc tccaacgcgc
     1861 acgaacgcga cgccgtctgg tcgcccgacg ggaagtggat cgcctacaat tccgatataa
     1921 ccggcgagaa cgagctctat gtccgtgcgc aggacggcaa aggccagccc cagcaagtga
     1981 ccaagggcgc cgacacttac tattatgcga tgctctggtc gcccgacagc aagaagctgc
     2041 tctggagcga tcgcctgcaa agattgcgtt acgtggatgt cgcgagcaaa gcagtcaccc
     2101 aggtcgacca ggacaaatat ggcgaagtcg ggtcctatga ttggtcgccc gacagtcagt
     2161 ggattgcctg gtcccggccg gaggacaatg ggctctccaa ggtctatctc tattccaccg
     2221 ccagcaaaaa gaccgtgccc gtgaccgacg attggtattc cgccggccaa tccaagttta
     2281 gcgacgatgg gaagtatctc ctttttactt cgagtcgcga cttcaagccc attttcggcg
     2341 aggaagattt tgccgatgtc tatcgcgaca tggagcgcat ctatctcgtg actctggcca
     2401 gggagacgga atccccgctc gccccgcgca gcgacgaggt gggcaaggcc gagaagaaag
     2461 gcaaggacgc cgaggaaaag aaacccgacg atgctgccaa aaagaaggac ggcaaagacg
     2521 agaagaagaa accggtggtg gtcaaggtcg atcccgatgg cctggggaat cggatcgatg
     2581 ccttcagcat tgcgccgggt aactacacag atctgcggat ggtcgatgac cgtcttttct
     2641 acctgcggcg cacagtcgcc gatgacaagg acgacgataa tgaggagggc ggcggccagg
     2701 atcgcaatgc gcacctttgt gcctacagcc tggaagaccg caaagagacc gttctcgggg
     2761 acgtcaacag ctatgagata acggccgacg gcaagaagat gctggtcaag atcaagaagg
     2821 attacgccat catcgatctg ccaaaagaca agctcgagac caaggatcac gagctcaagc
     2881 tcgccggcct cgacatgcag ctcgatcggc acgccgagtg gaaccaaatc tatttcgaag
     2941 cctggcggca gatgcgcgac ttcttctatt cgccaaccat gaatggcgtc gattggaagg
     3001 ccatgcgtga caaatatgcc gctcttcttc ccttcgtgaa ccatcgcaac gatcttacct
     3061 atctcatcgg tgagctgatc ggtgaattga ataacggtca cgcctatgtc gcgggcggcg
     3121 aacgccctga gactccgcgg atcaagctcg gattactcgg cgccgagttt tcccgtgacg
     3181 cggccacccg cgcttatcgc atcgaccgca tcttgcccgg cgagaattgg gaggacgaca
     3241 cgaagtcgcc gctcaccgcg atcggcctca acgtcaaggt tggcgattac atccttgcca
     3301 tcaacggcac gccggtcgct tccctgccga acatttacga cgccctcatc gggaccgccg
     3361 gcaagcaggt cattttgcgg atcaactccc agcctaacga ccaaggggcg cgcgatatta
     3421 cagtggtccc gatcgacgac gagtcgcctc tttattacct ggcctgggtc cggaagaaca
     3481 tcgattacgt gaaccggaaa accggcgggc aggtcggcta cgtccacatt cccgatatgg
     3541 gccagcccgg cttgaaccag ttcaccaagc tttacttccc acagattcgc aagaaaggac
     3601 tcatcgtcga cgtgcgcggc aacggcggcg gcttcgtctc gcccctcgtc atcgacaaac
     3661 tccgccgcgc cctggtcatg gtcgaaatgt cgcgcaacgg tgtggggcgg accgatccgc
     3721 ctaacacttt cctcggtcca atggccacgc tgatcaacga gttctccgcc tcggacggcg
     3781 atattttccc gttccgtttc aagacactcg gtctgggaaa attgatcggc aagcgcacct
     3841 ggggcggcgt catcggcatc cgcaacccgc ttccgctcgc cgatggcggc cagctcttta
     3901 agccggagtt cgcgccctat tcgaaagacg gcaagggctg ggtcattgaa agtcacggcg
     3961 tcgatcccga cattgtggtc gataacgatc cggcgaagga gtttcgcggc gaagatcagc
     4021 aactggatcg cgggatcgag gaggtccaga ccgagatgaa gaccaaggct tatgagttgc
     4081 cgcccatccc gcccttccca aatcgtaacc cggcaaatgg cggatcgagt agcacaaatc
     4141 cctagtgaca aaaaagatgt ttttgccgaa tcagaaagtc gtttgcgtga acggtcactt
     4201 tccgctcggc attgagaagc tctatgacga gctgccaaaa gagggcacga cctacaccat
     4261 ccgcgatatc gttccggg
//
LOCUS       DATMNS010000186         2845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_257610, whole genome shotgun sequence.
ACCESSION   DATMNS010000186 DATMNS010000000
VERSION     DATMNS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2845
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257610"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..526)
                     /locus_tag="VNW28_06325"
     CDS             complement(32..526)
                     /locus_tag="VNW28_06325"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXA09579.1"
                     /translation="MAASHSPNEMPDSPSLQERYAPNNACWGCGPANAEGLRIRSFPR
                     GDEVVAEWKPEPRYEAFPGVLNGGIIGTLLDCHCNWTAAYWLMGQAGEDHPPCTVTAD
                     YAIKLLRPTPTSDLVFLSAKVVDSSAARATVEGTLSAGGKICATCRGTFVAVKEGHPA
                     YHRW"
     gene            complement(526..1668)
                     /locus_tag="VNW28_06330"
     CDS             complement(526..1668)
                     /locus_tag="VNW28_06330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HXA09580.1"
                     /translation="MSTETKPAYSPGLAGVIAGETEICWVDPNAGLMYRGYDIHEMAE
                     KASFEEVAYLLLHDDLPTTEQRQEFSRAIAAERPLPRQVLEMLRLMPKNTHPMDMLRT
                     GVSMLAPFDPDLNDHSHQANIRKAIRLIAKVSTLITDGYRIQQGENPLPERSDLTLAA
                     NLFYKLSGEVPQAWKIRMMDTILILYADHEFNASTFAARVTASTLADMYAAVTSACGT
                     LKGPLHGGANEESMKMLDEIKTPDRAEGWMMDALARKAKIMGFGHRVYKKGDSRVPIM
                     REIGRDLGKRVGKEQWVPICEKLEATMEREKQLCANVDLYAAPVFTMLEFPPALNTPI
                     FAASRVAGWCAHVVEQHDHNRLIRPRSLYTGPAARPYKGVSPNGAR"
     gene            complement(1740..2624)
                     /gene="prpB"
                     /locus_tag="VNW28_06335"
     CDS             complement(1740..2624)
                     /gene="prpB"
                     /locus_tag="VNW28_06335"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: protein motif:HMM:TIGR02317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HXA09581.1"
                     /translation="MRQLIAQGVVALPGVPNASMARQVERAGFDAVYISGAGLANCTA
                     GLPDVGLLTLNEVATLAGFIAHAVKIPAIVDADTGFGGAENVGRTIYELERAGLAGCH
                     LEDQEFPKRCGHLAGKRLVETEEMAAKIRAAVAAKRDCDFLVIARTDSRALENFDGAV
                     QRANAYVAAGADAIFPEALQTKEEFRDFAREVKVPLLANMTEFGKSPLLNVRELGELG
                     YRMVIFPQSAFRVAMKTTGDFFRALKETGSAEAWLDRMQTRQELYETLDYDPAAESWP
                     GYHADVEKRTSNPESFRG"
BASE COUNT          510 a          908 c          845 g          582 t
ORIGIN      
        1 ttttcgatct gcgcggcgca gggaaggctg cttaccatcg atgataggcc gggtgccctt
       61 ctttcaccgc cacaaaggtt ccgcggcaag tcgcacagat ttttccgccg gcactgagcg
      121 ttccctcgac cgtggcccgc gcagcggatg aatcgaccac tttcgcggaa aggaagacca
      181 aatcgctggt tggcgtgggg cggagcaact tgatcgcgta atcagcggtc accgtgcagg
      241 gcggatgatc ctcgccagcc tggcccatga gccaatatgc cgccgtccag ttgcaatggc
      301 aatcgagcag cgtgccgata atgccgccat tcaggacacc ggggaacgcc tcgtagcgcg
      361 gctccggttt ccattccgcg accacttcgt cgccccgcgg gaagctgcgg atgcgcagac
      421 cttccgcgtt cgccgggccg cagccccagc aggcattgtt cggcgcgtag cgctcttgca
      481 gggatggcga atccggcatc tcgttaggcg agtgcgacgc ggccatcacc tggcgccgtt
      541 cggcgaaacc cctttgtaag ggcgcgccgc tggtccggtg tagagcgagc gcggccggat
      601 caggcggttg tgatcgtgct gctcgacgac atgcgcgcac cagccggcaa cgcgggaggc
      661 ggcaaaaatc ggcgtgttca acgccggcgg gaattccagc atggtgaaga ctggcgcggc
      721 gtagagatcg acattcgcgc agagttgctt ctcccgctcc atcgtcgctt cgagcttttc
      781 gcaaatcggc acccactgct ccttgccgac ccgtttgccc aggtcgcgcc cgatctcgcg
      841 catgatcggc acgcgcgaat cgcccttttt gtaaacgcga tggccaaagc ccataatctt
      901 cgctttccgg gcgagcgcat ccatcatcca gccttccgca cggtccggcg tcttgatctc
      961 gtcgagcatt ttcatcgact cttcgttcgc tccgccgtgc agcggtccct tgagcgtgcc
     1021 acaggcgctg gtgacggcgg cgtacatgtc ggccagggtc gaagcggtga cccgcgcggc
     1081 gaaggtcgaa gcgttgaatt cgtgatcggc gtagaggatc aaaatcgtgt ccatcatcct
     1141 gatcttccat gcctggggca cctcgccgct gagtttgtag aagagattgg ccgccagcgt
     1201 caggtccgat ctttccggca gcggattttc gccctgctgg atgcgataac cgtcggtaat
     1261 gagagtggag accttcgcga tcaagcggat ggccttgcgg atattggcct ggtgcgagtg
     1321 gtcgttcagg tcgggatcga acggagcgag catcgacacc ccggtgcgca gcatgtccat
     1381 gggatgggtg tttttcggca tgaggcgcag catctccagg acctgcctgg gaagcggacg
     1441 ctccgcggca atggcgcggg agaattcctg ccgctgttct gtcgtcggca gatcgtcgtg
     1501 gagcaggaga taagcgacct cttcaaagct cgctttctcc gccatctcgt ggatgtcgta
     1561 gccgcgatac atcagaccgg cattgggatc gacccagcag atttcggtct cgccagcgat
     1621 cacgccggca aggcctgggc tgtaggcggg tttggtttca gtgctcatag actctcctgc
     1681 gtgcggttca tattcttaac gtccaacgtc caacgttcaa ggccttcgcg agcaggcgtt
     1741 caaccccgaa agctttcggg gttggacgtt ctcttctcca catcggcgtg atatcccggc
     1801 cagctttctg ccgccggatc gtaatcaagc gtctcgtaaa gttcctggcg cgtctgcatc
     1861 cgatcgagcc acgcttccgc cgagccggtt tccttcaagg cccggaaaaa atcgccggtc
     1921 gttttcatcg ccacgcggaa agcgctctgc gggaagataa ccatccgata tccgagctcg
     1981 cccagttctc tcacgttaag gagcgggctt ttgccaaact ccgtcatgtt ggcaagcagc
     2041 ggcaccttca cttccctggc gaaatcccgg aactcttcct tcgtttgcaa tgcttccggg
     2101 aagatcgcat ccgcaccggc ggcgacgtag gcattcgccc gctgcaccgc accgtcgaaa
     2161 ttttccagcg cccgcgaatc ggtccgcgcg atgacgagaa aatcgcaatc ccgtttcgcc
     2221 gcgaccgcgg cgcggatttt cgccgccatt tcctcggtct cgacgagtcg cttgccggcc
     2281 agatgcccgc agcgcttcgg aaattcctga tcttccagat ggcagccagc caaaccggcg
     2341 cgctccaact catagatcgt gcgcccaaca ttttcggcgc cgccgaaacc ggtatccgcg
     2401 tccacaatcg cgggaatctt caccgcgtgg gcgatgaagc cggcgagcgt ggcgacttca
     2461 ttcaaagtca gcagcccgac atcgggcaag ccggcagtgc aattcgccag ccccgcgcca
     2521 ctgatgtaaa cggcgtcgaa tcccgcgcgt tccacctggc gcgccatgga ggcgtttggc
     2581 acgcctggca gggcaactac gccctgcgca atcagctggc gcaagcggct cgttattccg
     2641 agcgaagtcg aggaatcccg cggcatagcc aaacagttcg ttaggctggc atctgcgcca
     2701 gcttcattaa ttcgtgaagg ttggacgtct cctccagttt ccaacccgcc gccaaaattt
     2761 tcccggcgcc ctcctcgccc agttccggga cgaccagcgc gttgaacttt ccttccagtt
     2821 gccggtcgct caccgggttg aaggc
//
LOCUS       DATMNS010000187         6515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_974841, whole genome shotgun sequence.
ACCESSION   DATMNS010000187 DATMNS010000000
VERSION     DATMNS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974841"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VNW28_06340"
     CDS             <1..1165
                     /locus_tag="VNW28_06340"
                     /inference="COORDINATES: protein motif:HMM:NF015279.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HXA09582.1"
                     /translation="IARYLINDKVSALMASPVRILTAVPICDGHDSAINTINLEFIRH
                     GIEVVYLGYHRSVEDIVRTAIQEDVGAVGISSYNGGHVEFFSEVARALRKQGADDIKL
                     FGGGGGTITEADAGVMRRNGVDKIFFAGTALTAMADFVRAHYGNTHRRRRKATPDMKL
                     ARALTEIEDRYAKNRRARKPAKKKSKSLGKTRVIGFTGPGGAGKTTLIDELVLRLLRR
                     APQARIAILSHDPSVVGEGALLGDRATMINSQNDRVFMRSMATRGQAGGLSPATGECL
                     ALLAGSGFDYVILETVGTGQEAMPFQNELVQKTVLVMNPDYGSRLQLQKIVMLDLADL
                     VVVNKSDQSRARTAVSEIEQRLEQNKRAQKLLSTVAKRHRDAGVDQLCDWVMD"
     gene            1345..3135
                     /locus_tag="VNW28_06345"
     CDS             1345..3135
                     /locus_tag="VNW28_06345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXA09583.1"
                     /translation="MLEEVKRARSEKKFGQQMVDRWNDVKAKTPIGTAPTGLKLPRLA
                     LPEIDEAGEITRYLLGEGLPGEFPYTNGAYREMYLEPLREPEVGSFGKAGQNGRNGHR
                     KNGKKAPALEKTRQTEEPTRLFSGFGLAEDTNKRFHYLTGHQRSARLSTAFDGPTLYG
                     IDSDADGVFGKIGEGGLAIDTYEDMVRLYDGFDMGAPDFSASMTISGPAPIIMAMYIA
                     AAKKRFGPKVVPNLRGTIQADIFKEVQAQNETIFPTEASLRFLCDMIEYTTREMPRWY
                     PVSISGYHIGEAGATPVQQAAYTVSNGFAYAEMLAARGIPVDQFAPRLSFFLDCGLDV
                     EYIALARASRRIWAIGMRDVFGAGPKGQMFKLHTQTSGRSLVAAEFKNNLTRTAAELM
                     LAYMNGTNSCHSNSADEPFTTPSEEWIRLAAHGQAILLDESGIFKHTMNMLTGSPGMK
                     AVERAVEAAMLEEFRQIEELGGVLPAIEHRYQRSQIQTAAHRYEEQIYNGTRPIIALN
                     KYNDRKDEMPEVKMVRTPRGKKQLQVDRLAKFKKRNAAQAERALDQLTAVVESGENFF
                     PTLIETVEVCSLGQITSRLQEVVGRFRPMV"
     gene            3184..3678
                     /locus_tag="VNW28_06350"
     CDS             3184..3678
                     /locus_tag="VNW28_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXA09584.1"
                     /translation="MPDPHSKNLRLHRLRDAPSTFFVTKSLRPKRPVLGSELRQINVE
                     AFAFAVQHERIYLRAFVVMPDHWHALLGLREPWTLPKFMHALMSHVGAQSSMRLTKHQ
                     TAWQDGYYETLIKTARQFMYVAIYIEENPVKKGLVTTPEEWEESSAARKDLVTDQWPW
                     VFDS"
     gene            complement(3725..4144)
                     /locus_tag="VNW28_06355"
     CDS             complement(3725..4144)
                     /locus_tag="VNW28_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09585.1"
                     /translation="MKIKAVLLLATVLCLGLPPLIQGKKATGSSWTGNYTDQKYCNGQ
                     AVFELNIVQEGGTITVDFDAAYHDGHGCAPEGSGSAKVVDKNTLQFTFTDSANNAGVG
                     TIKRVGDGVLISITPNRVADPRCVIFYGENIRLKPAR"
     gene            complement(4269..5453)
                     /locus_tag="VNW28_06360"
     CDS             complement(4269..5453)
                     /locus_tag="VNW28_06360"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXA09586.1"
                     /translation="MILEHPLSDAESAFLDRVAAFSRDEVMPHADNWDQDEKLPREIF
                     TKAGQIGMMGMAAPKEYGGGGMSNVTAALAIKELGKAYAALSMDIAAHNALAVGQINR
                     FGTEDQKKKYLPRLTNGDWIGAWALTEPNAGSDTGGIETKGTEEGGQWRINGMKKFIT
                     SGRTADLVVVMATTGQTDKGKNEISAFILMKDQVTPVRKIHTYGMRASDTAELRFENA
                     KAELLGERGRGREQALTVLDRGRIGIAALAVGIAEAAFERANSYANERKQFGHAIGDF
                     QAVQWMLVDSAMELEAAEVMTLHAAYLQDRGESTTKESAMAKLMASESAVRICDRSMQ
                     IHGGYGYSRDLPIERYLRDAKLCEIGEGTSQVQRLVIARHVLKEAKPLKLAHREPRAP
                     NT"
     gene            complement(5611..>6515)
                     /locus_tag="VNW28_06365"
     CDS             complement(5611..>6515)
                     /locus_tag="VNW28_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HXA09587.1"
                     /translation="EVSLVKAWKGDKSGNLIFRKTSRNFNPMIASCGRVCVAEVEHLV
                     EAGELDPDQIHTPGIFVDRIIQGAKYEKRIEFRTVRGGAAKKENPIRELMAKRAAQEL
                     RDGYYVNLGIGIPTLVANYIPQGMNVTLQSENGLLGIGPFPTEDEVDADLINAGKQTI
                     TTMPGSAFFSSADSFTMIRGGHIDLAILGAFEVTDKGDIANWMVPGKMVKGPGGAMDL
                     VAGVKRVVAVMEHCARDGSPKILKKCTLPITGLGVVNLIVTDLCVFEVKEGGGLVLTE
                     LHGDATVDDIRAKTGAQFEVALKS"
BASE COUNT         1277 a         1907 c         2030 g         1301 t
ORIGIN      
        1 gattgcccgg tatctgatta acgataaagt ttcggcgctc atggcttcac ccgtccgtat
       61 tctcaccgcc gttccgattt gcgatggcca cgacagcgcg atcaacacca tcaatctcga
      121 gttcattcgg cacgggatcg aggtcgttta tctcggctat catcgctcgg tcgaagacat
      181 cgtgcggacc gcgatccagg aggacgttgg cgcggttggg atctcgagct acaacggcgg
      241 acatgtcgaa tttttctccg aagtggcgcg cgcgttgcgc aaacagggcg cggacgacat
      301 caagcttttc ggtggcggcg gcgggacaat cacggaagcg gatgcggggg tcatgcgccg
      361 gaacggcgtc gataagatct tcttcgccgg cacggcgctg accgcgatgg cggatttcgt
      421 gcgcgcgcat tacggcaaca ctcatcgccg gcggcgcaaa gcgacgccgg acatgaagct
      481 ggcgcgcgcg ctgacggaaa tagaagatcg ttacgcgaaa aacaggcgcg cccggaagcc
      541 ggcgaagaag aaatcgaaat cgttaggcaa gacccgggtg atcggattta ccgggccggg
      601 cggcgccggg aagacgacgc tgatcgatga actggttctg cgcctgctcc gccgtgcgcc
      661 gcaagcgcgc atcgccatcc tctcgcacga tccgagcgta gtcggcgaag gcgcgctcct
      721 cggcgaccgc gcgacgatga tcaattcgca gaacgatcgc gtcttcatgc gcagcatggc
      781 gacgcgcggg caagccggcg gattgtcgcc ggcaacgggg gaatgcctcg cgttgctcgc
      841 agggtcgggt ttcgactacg tcattctgga gacagtgggc accggccagg aagcgatgcc
      901 cttccagaac gagctcgtcc aaaagacggt gctcgtgatg aatcccgatt acggcagtcg
      961 cttgcagctc cagaaaatcg tgatgctcga tctggccgat ctcgtggttg tgaacaagag
     1021 tgatcaaagt cgcgcgcgca ccgcggtttc tgaaatcgag cagcggcttg agcaaaataa
     1081 gcgtgcgcag aaattgcttt cgacggtcgc gaagcggcat cgcgatgcag gggtggatca
     1141 attgtgcgat tgggtgatgg attaggttcg tcatgtcgag cgtcgtcgag acatcccgtg
     1201 gagttaccct tgaagggtca cgcaccggga tccctcggct gcgctcggga tgacggggcg
     1261 gcgtgaactt gaagagcgaa gaaggaaaca aatatgagca agttgggacg agtcgttgag
     1321 gcagtggaaa attataatcg gttcgtgctt gaggaagtga agcgcgcgcg ttccgagaag
     1381 aaatttggcc agcagatggt cgatcgctgg aacgacgtga aggcgaaaac gccgatcgga
     1441 accgcgccca cgggattgaa gttgccgcgc ctcgccctgc ccgagatcga cgaagccggg
     1501 gagatcaccc gttatttgtt aggcgaaggt ctgcccggcg aatttcccta caccaacggc
     1561 gcctatcgcg agatgtatct cgagccgttg cgcgagccgg aggttggcag tttcggcaag
     1621 gccggacaga acggtaggaa tggtcatcgc aagaacggga aaaaggcccc cgccctcgaa
     1681 aagaccaggc agaccgaaga accgacgcgg ctcttctccg gtttcggttt ggccgaggac
     1741 acgaacaaac gttttcatta cctgaccgga caccagcgca gtgcgcgcct cagcacggcc
     1801 ttcgacggcc cgacccttta cgggatcgac agcgacgcgg acggcgtctt cgggaaaatc
     1861 ggcgagggcg gtctggcgat cgacacctac gaagacatgg tgcggcttta cgacggcttc
     1921 gacatgggcg cgcccgattt ttccgcctca atgacgatca gcggccccgc gccgatcatc
     1981 atggcgatgt atatcgccgc ggcgaaaaaa cgcttcggcc cgaaggtcgt cccgaatctg
     2041 cgcggcacga tccaagccga cattttcaag gaagtgcagg cgcagaatga aacgattttc
     2101 ccgacggaag cgtcgcttcg ttttctttgc gacatgatcg aatacacgac gcgcgaaatg
     2161 ccgcgctggt atccggtttc aatcagtggt taccacattg gcgaggcggg cgcgacgccg
     2221 gtgcagcagg cggcctacac cgtttcgaac ggtttcgcct acgcggaaat gctcgcggcg
     2281 cgcggcattc cggtcgatca atttgccccg cggctctcgt tctttttgga ttgcgggctc
     2341 gatgtcgaat acatcgcgct cgcgcgcgct tcgcggcgga tttgggcgat tgggatgcgc
     2401 gacgtgttcg gtgcgggacc gaaaggtcaa atgttcaagc tccacaccca gacgagcggc
     2461 cgatcgctgg tcgcggcgga attcaagaac aacctaacga gaaccgcggc cgagttgatg
     2521 ctcgcttaca tgaacggcac caactcctgc cactcgaaca gcgccgacga accgttcacc
     2581 accccgagcg aagaatggat ccggctggcg gcgcacggac aggcgatcct gctcgacgag
     2641 tccggcatct tcaaacacac catgaacatg ctgactggtt cgcccgggat gaaagcggtc
     2701 gagcgcgccg tcgaggcggc gatgctcgaa gagttccggc agatcgagga actgggcggc
     2761 gttttgccgg cgatcgagca tcgctatcag cggtcgcaga ttcagaccgc ggcgcatcgt
     2821 tacgaggagc aaatctacaa cggcacgcgg ccgatcatcg ccttgaacaa gtacaacgat
     2881 cgcaaagacg agatgccgga agtgaagatg gtgcgcacgc cgcggggcaa gaagcagtta
     2941 caagtcgatc gcctggccaa gttcaaaaag aggaacgcgg cgcaagcgga gcgcgccctc
     3001 gatcaactga ccgcggtggt ggaaagcggg gaaaatttct tcccgacgct gatcgagacg
     3061 gtcgaagtct gttcgttagg ccaaatcacc agccggctgc aggaagtcgt ggggcgtttc
     3121 cgtcccatgg tgtagcgacg acttcaatgt gggaggggcc tgtgtgcccc gattggtggc
     3181 gacatgccag acccccattc caaaaacctg cgtcttcacc gcctgaggga tgctccatcg
     3241 acatttttcg tcacgaaatc cttacgaccg aagaggccgg tgcttgggtc tgagcttcgg
     3301 caaatcaatg tggaagcgtt cgccttcgcc gtgcagcacg agcgtattta cctccgcgcg
     3361 ttcgtcgtca tgccggacca ttggcatgcg ttgcttggac ttcgcgaacc ttggacgctg
     3421 ccgaaattta tgcacgcgct catgagtcat gtcggagcgc agagcagcat gcgcctaacg
     3481 aagcaccaaa ctgcctggca ggatggctac tacgagactt tgatcaagac tgcgcgccag
     3541 ttcatgtatg tggcaatcta tatcgaggag aatccggtca agaaagggct cgtgacgacg
     3601 ccggaggaat gggaggaaag tagtgcggcc aggaaggatc ttgttaccga tcagtggcct
     3661 tgggtgtttg attcgtaagt cctcagtgcc ggcgatcggg gcacacaggc ccctcccaca
     3721 ttgtttatcg cgccggcttg aggcggatgt tttccccgta gaaaataaca cagcgcgggt
     3781 ccgctacgcg atttggcgtg atcgagatga gtacgccgtc gcccacgcgc ttgatcgtgc
     3841 cgacgccagc gttgtttgcg ctgtcggtaa aggtgaactg tagcgtgttc ttatcgacga
     3901 ctttggcgga tccgctgccc tcgggtgcgc agccgtgtcc gtcatggtaa gcggcgtcga
     3961 aatcaactgt gatcgtccca ccttcctgca cgatgttgag ctcaaagacc gcctgaccat
     4021 tgcaatattt ctggtcggtg tagttgccgg tccagctgga tcccgtcgct tttttccctt
     4081 ggataagtgg gggcaaccca aggcagagaa cggttgccag caagaggacg gctttaattt
     4141 tcatggccga cattgaacca gggtatcaca ggcttccagc ctgccaggta aagcgggcgt
     4201 ctcgcccgct gttcacacag cgtgcaggcg ggacgccccg cacgacgcga caggcgggga
     4261 cgcctgcgtt acgtgttcgg cgcgcgcggt tcgcggtggg cgagcttgag cggcttcgct
     4321 tccttcaaga cgtggcgcgc gatgacgagt cgctgcacct gcgaggtgcc ttcaccgatt
     4381 tcacaaagtt tcgcgtcgcg cagatagcgc tcgatcggca gatcgcggct gtagccgtag
     4441 ccaccgtgga tctgcatcga gcggtcgcag atgcgcaccg ccgattccga agccatcaac
     4501 ttcgccatgg cggattcctt ggttgtgctc tccccccggt cctgcaggta agcggcgtgc
     4561 agcgtcatga cttccgccgc ctccaattcc atcgcggaat cgaccagcat ccattgcacc
     4621 gcctggaaat cgccgatcgc gtgcccgaat tgctttctct cgttcgcata ggaattggcg
     4681 cgctcgaagg ccgcttccgc gatcccaacc gcgagcgccg cgatgccgat gcggccgcgg
     4741 tcgagcacgg tcagcgcctg ttcacggccg cggccgcgtt cgccgagcaa ttccgctttc
     4801 gcgttctcga aacgcaactc ggccgtgtcg ctcgcgcgca tcccgtaggt gtggattttt
     4861 cgcaccggcg tcacctggtc tttcatcagg atgaaggcac tgatctcgtt cttgcctttg
     4921 tcggtctggc cggtggtggc catgacgacg acgagatcgg cggtgcggcc actggtgatg
     4981 aattttttca ttccattgat ccgccactgg ccgccttcct cggtcccttt ggtctcgatc
     5041 ccgccggtgt cgctgcccgc gttcggctcg gtcagggccc aggcgccgat ccagtctccg
     5101 ttcgttaggc gcggaagata ttttttcttt tggtcctcgg tcccgaaacg attgatctgg
     5161 ccgacggcga gggcattgtg cgcggcgatg tccatggaga gcgcggcgta ggctttgccc
     5221 agttccttga tcgcgagcgc ggcggtcacg ttgctcatcc cgcccccgcc gtattccttc
     5281 ggtgcggcca ttcccatcat gccgatctgc cccgctttgg tgaaaatttc gcgcggcaat
     5341 ttttcgtcct ggtcccagtt atcggcatgc ggcatcactt cgtcgcggga aaaggcggcg
     5401 acgcggtcga ggaaggcgct ctcggcgtca ctcagaggat gttcgagaat cataggcgca
     5461 agatagtccg ccagcgtagc gctcccggat gttaggagcg aaaagaaaaa gcgcggcgcg
     5521 aagatgcccg ggtgacgcac gcgtcgcgcg agcgcaaccc tggcggaggc cagcaaggcg
     5581 cggagcggag aacacgcgcc tcgattcgcg ttagctcttg agcgcgacct cgaactgggc
     5641 gcccgtcttg gcgcggatat cgtccaccgt cgcgtcacca tgcagctcgg tgaggacgag
     5701 tccgccgccc tctttcacct cgaagacgca caggtcggtg acaatgaggt tcacgacgcc
     5761 caggcccgtg atcggcaacg tgcatttttt caaaatcttc ggactaccgt ccctggcaca
     5821 gtgctccatt accgccacga cgcgcttcac cccggcgacg aggtccattg ctccgccggg
     5881 ccccttcacc atctttccgg ggaccatcca gtttgcgatg tcgcccttat ccgtcacttc
     5941 gaacgcaccg aggatggcga gatcgatgtg tccgccgcga atcatcgtga aggagtcggc
     6001 gctcgagaaa aaggccgagc cgggcatcgt cgtgatggtc tgcttgccgg cgttgatcag
     6061 atcggcgtcg acttcgtcct cagtcgggaa cgggccgatg ccgagcaggc cgttctcgga
     6121 ttgcagggtg acgttcatac cttggggaat gtaattggcc accagtgtcg ggatgccaat
     6181 gccaaggttg acgtaataac cgtcgcgcaa ttcctgcgcc gcgcgtttcg ccatcaattc
     6241 gcggatgggg ttttctttct tcgccgcgcc gccccgcacg gtgcggaact cgatccgttt
     6301 ttcgtatttc gccccttgga taatgcggtc gacgaaaatg cccggcgtgt ggatctggtc
     6361 gggatcaagt tcacctgcct cgacgagatg ctctacttca gcgacgcaga ccctgccgca
     6421 ggacgcgatc atcgggttga agttccggga ggtttttcga aaaatgaggt tgccggattt
     6481 gtcgcccttc cacgctttca cgagcgaaac ctcgg
//
LOCUS       DATMNS010000188        15953 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4911251, whole genome shotgun sequence.
ACCESSION   DATMNS010000188 DATMNS010000000
VERSION     DATMNS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 15953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15953
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4911251"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1409
                     /gene="mnmG"
                     /locus_tag="VNW28_06370"
     CDS             <1..1409
                     /gene="mnmG"
                     /locus_tag="VNW28_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HXA09588.1"
                     /translation="FLRGLLHVGDRSQAGGRMADSSSPLSDNLRELGFRTGRFKTGTP
                     CRINARSIDFAACSKQLGDEPPSRFSYLPVDEEFEDDEISALNRVRNGSFHVEQMPCW
                     ITATTPETHEIIRRNLHRSPLYSGRIEGTGPRYCPSIEDKVVKFSDKPQHQLFLEPEG
                     RQTQEFYINGVSTSLPYEVQIEFIRSIPGLQNAQIMRPGYAVEYDYFPPTQLQSTLET
                     KLVSRLYFAGQINGTSGYEEAAAQGLIAGANAALELAGQPALVLDRSEAYIGVLIDDL
                     VTRGTQEPYRMFTSRAEHRLLLRQDNADQRLTAKAASLGLVGHRRMEILEKKSRLLQK
                     AREIAATTRLEGQTLGHSMKHPEFHVQTLPRGLLREVDAEIWELLETELKYEGYIRRQ
                     NEQLRATHLAESVRIPAQIDYSVVPGLRNEARQKLAGLRPESIGQAGRISGVTPSDLG
                     ILTIWLSKQNRTCLQSTS"
     gene            1413..2045
                     /gene="plsY"
                     /locus_tag="VNW28_06375"
     CDS             1413..2045
                     /gene="plsY"
                     /locus_tag="VNW28_06375"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXA09589.1"
                     /translation="MHYPILSYLIAVAIGYLLGSCPNGYLVSRTRGIDIRKQGSGNIG
                     ATNVLRVLGKKWGYLVFALDTLKGFVAVRLAFAVVLALTPHTTQRELIGIAGGLACIL
                     GHTFPIWLRFRGGKGVATSAGVLLGLMPIAVFSVFAVWLILFQVTRYVSVASITAAAA
                     LPVFVMLYLHLKLLTGASLLPFSILIAGVVIWRHRSNMRRLFHGKEQRFG"
     gene            2056..3051
                     /locus_tag="VNW28_06380"
     CDS             2056..3051
                     /locus_tag="VNW28_06380"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: protein motif:HMM:NF000942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HXA09590.1"
                     /translation="MGGIAILGTGGWGTALAVLWASHGNDIILWGHTPERVKEISRSR
                     QNPDYLPAVELPPNVCLTSSLADCTAADLIVFVTPSVALREVAAKLREELTSESAILM
                     SCTKGIEHGTGMRMSEILSERFPQNPVSVLSGPNLAVEVARGLPSATVLGCRDETVAE
                     QLQSTLGSSRFRVYTSDEIVGIELGGALKNIFAIPAGVSDGFGFGDNSKAALLTRSLA
                     ELFRLGVAMGGDARTFQGLSGAGDLIATCFSRHSRNRGLGEQLGRGKSLAEITRGMKT
                     VAEGIPTARSAFECARKLGVETPIIDQVYAVLHEGKSPAQAMQELLGRDQKSERG"
     gene            complement(3048..3644)
                     /gene="purN"
                     /locus_tag="VNW28_06385"
     CDS             complement(3048..3644)
                     /gene="purN"
                     /locus_tag="VNW28_06385"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXA09591.1"
                     /translation="MSRLALGILGSGKGSNCRAILEKIRLGELAADCRIVVSDVADAG
                     ILAAAREFGVPDVFLPPGKFRTKLEPEVEQQLVRLVRGAGVELIVLAGFMRVLKSTVL
                     EAFPGRIINLHPSLLPKFPGREAWTQALSAGEKITGCTVHYVDAGVDSGEIIAQREVP
                     ILPNDTPATLHERIQLAERELFPAVIADLAAKMAAAKS"
     gene            complement(3641..4126)
                     /locus_tag="VNW28_06390"
     CDS             complement(3641..4126)
                     /locus_tag="VNW28_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HXA09592.1"
                     /translation="MKTKILSLSELSRRADEVRQARQRLVLTNGCFDLLHVGHVRYLK
                     QARELGDFLAVAVNGDESVRALKGAGRPLNSAQDRAEVLAALECVDFVTIFPDLRATQ
                     VIKATRPAIYVKGGDYTLESLNAEEVGALQEAGVEIKTLPLVPGKSTSGLIARMHPPS
                     A"
     gene            complement(4207..4617)
                     /locus_tag="VNW28_06395"
     CDS             complement(4207..4617)
                     /locus_tag="VNW28_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09593.1"
                     /translation="MNHKRGVIALIVAFVFIFFFGFVWHGILMKSAYTETAALWRADP
                     DFNSHFWILLLGHAVMAFAFTGLYVSKVGVQSMATGLGYGIVFGVFCTGIDLVRFAVE
                     PLTTYILWMWIGGSLIGFAIMGALVGAIYKPQAA"
     gene            4907..6646
                     /gene="ggt"
                     /locus_tag="VNW28_06400"
     CDS             4907..6646
                     /gene="ggt"
                     /locus_tag="VNW28_06400"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HXA09594.1"
                     /translation="MLSPNRLAIAIVALLVLDFPLQAQIDRITGKNFATRSEVLARHG
                     MVCTSVPLATQVGLDILKRGGSAVDAAIAANATLGLMEPVSNGVGGDLFAIVYSAREN
                     KLYGINGSGRSPLGLSYDQMKAELAKLKRETIPPTGMLPISVPGCVDAWSELHRKFGN
                     LKLGDDLAPAIQYAEEGFPVTELIAYYWGFGPHIYKGLPGAFLETYTLDGKGRTPAKG
                     DIFKNPALANTLRLIGEKGRDAFYKGEIADKIDAFMQANGGYLRKADFERHTSTWVDP
                     VSTNYRGYDVFELPPNGQGIATLQILNILEGFDLKAMGRNSPETLHAMIEAKKIVWAD
                     RARFYADPAFAKIPLAGLLSKNYAAERRKLIDPNHAAKKVGAGNPPTLDQGDTIYLCT
                     ADHEGNMVSLIQSNYRGQGSGIVVPGLGFMFQDRGELFSMEPGHANAYGPGKRPFHTI
                     IPGFVMKDGKPWEAFGVMGGGMQPQGHVQVLTNQIDFGLNVQEAGDASRWQHEGDNEP
                     TGEKMENGGYVELESGIPYETARELRKRGHDVRLDVGGYGGYQAIKVEVHDGQRVYVG
                     ASESRKDGQAAGY"
     gene            6932..7246
                     /locus_tag="VNW28_06405"
     CDS             6932..7246
                     /locus_tag="VNW28_06405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09595.1"
                     /translation="MARQFKPVRFFVMMAAVAFIVCGVTSFFTHRAEHGRTSEERSAY
                     FIGEKAGEEAAPGGQLPTAAALNIMAQQSFERQGSGEKENWDLAFEHGYEDGFKKTHS
                     AP"
     gene            7352..7825
                     /locus_tag="VNW28_06410"
     CDS             7352..7825
                     /locus_tag="VNW28_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXA09596.1"
                     /translation="MQRITPFLWFDDQAEEAASFYVSVFKNSKVKAMTHYTGEEPSGQ
                     KGSVMTVDFELDGQDFVALNGGPRFKFTEAVSFVIDCETQEEIDYYWEKLTADGGEEV
                     QCGWLTDKYGLSWQVVPNKWAEWARNAAGMQRLMHALMPMKKLDIAALQKAYDGK"
     gene            complement(7883..8677)
                     /locus_tag="VNW28_06415"
     CDS             complement(7883..8677)
                     /locus_tag="VNW28_06415"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HXA09597.1"
                     /translation="MSDKPSKLKIADREFTSRLLVGTGKFASNELMRDALDASGTELV
                     TVALRRADLSGKGDPFANILDFIDPKRFLLLPNTSGARNADEAVRLARLAVTAGLPSW
                     IKLEIHPDPRYLLPDPVETLAAATILVKEGFTVLPYINADPVLAKRLQDVGTATVMPL
                     GSPIGSNRGIETRAQIEIIIEQATVPVVVDAGLGAPSQAAEALEMGADAVLVNTAIAI
                     ASDPVRMAHAFRKAVEAGRDAREIGLAETRTTASATSPLTAFFSEA"
     gene            8888..9637
                     /locus_tag="VNW28_06420"
     CDS             8888..9637
                     /locus_tag="VNW28_06420"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HXA09598.1"
                     /translation="MAQLETASIDLVVTSPPYNLGIHYGKFADTADRETYLDWCEQWA
                     AQIRRLLQPNGSLFLNVGSAPSNPMLPHELILRLRKIFVLQNTIHWIKAITIPDETGE
                     EISRGHFKPISSPRFLNDCHEYIFHLTPNGKTPLDRLALGVPYADKSNIARWSHTRGR
                     DRRCRGNTWFIPYETIQRRAKERPHPATFPVQLATNCIRLHGLTRTQSMLDPFLGIGN
                     SAAAARRAGLTNFVGFEIDEEYLAEARRRIG"
     gene            complement(9786..10814)
                     /locus_tag="VNW28_06425"
     CDS             complement(9786..10814)
                     /locus_tag="VNW28_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HXA09599.1"
                     /translation="MQLLTLKEIRQAVPQGALEARVHVQLETVAAKFTREQKPYCELT
                     LADAADRMTLRVWSDSPNFKACDAMQSGDFVEITGEFYQHQQYGLDARQWKSRPLTAE
                     ERAHLLQGPAELRAKQAADWEFIVATTAAIRDPRLYSLCAEFIAQYGARFRNTAGART
                     YHHARRGGLVEHTAQMMRVATEIAPQYPGLNLDLLVAGILFHDAGKLWENYLPETGFS
                     MPFDELGEMVGHIGIGLEVVNSLWQKIKADGRAAEWQKLSPPTEDCRLHLLHLIASHH
                     GEPQFGSPVYPKTPEALALNYIDNLDARLEMIFAGYAVAKPLAGRIFERVRPLPGNLI
                     TPLAKFSG"
     gene            complement(10927..11463)
                     /locus_tag="VNW28_06430"
     CDS             complement(10927..11463)
                     /locus_tag="VNW28_06430"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HXA09600.1"
                     /translation="MKTNQLRAAICLLAAGSLSLLTATAFAGEITKAIAVLHPTKGSN
                     VEGTVTFTKSGDEIKIVADVTGLTPGKHGFHIHEFGDCSSPDGKAAGGHFNPTNSPHA
                     GHDAAQRHEGDMGNLEADSAGKAHLELTDNMMTMSGEKSIIGRGVIVHEKEDDLKSQP
                     VGNAGARVACGVIGIAKP"
     gene            complement(11521..12405)
                     /locus_tag="VNW28_06435"
     CDS             complement(11521..12405)
                     /locus_tag="VNW28_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HXA09601.1"
                     /translation="MPNDSSRVALIQMQCGPEPEANFAKAVARVREAARQGAQIICLP
                     ELFRSQYFCQSEDHANFALAEPIPGPSTQSLGEVARETGAIIIASLFERRAAGLFHNT
                     AAIIGGHGELLGKYRKMHIPDDPSYYEKFYFTPGDLGFQAWPTPQGRIGVCICWDQWY
                     PEAARLTALRGAEILFYPTAIGWHPAEKAKYGYAQHSAWETMQRSHAIANGCFVAAVN
                     RVDHEAPAGGDGIEFWGQSFIVAPSGEILAKASVDREEIIFADVEWKRVNEHRTHWPF
                     LRDRRIDAYAGLEQRLLD"
     gene            complement(12407..12934)
                     /locus_tag="VNW28_06440"
     CDS             complement(12407..12934)
                     /locus_tag="VNW28_06440"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXA09602.1"
                     /translation="MDTNQKEVAVLKTSDGEMVVEFWPEVAPKTVENFKKLARQGFYD
                     GTAFHRIIKGFMIQGGDPNTKDLANESSYGTGGPGYKIPAEFNDHPHVRGVLSMARSA
                     DPNSAGSQFFICLGTASSLDHQYTAFGKVIKGLDVLDKIGDTPVTRSAMGEASKPTTR
                     VALESVKIAPAASVK"
     gene            complement(13156..13965)
                     /locus_tag="VNW28_06445"
     CDS             complement(13156..13965)
                     /locus_tag="VNW28_06445"
                     /inference="COORDINATES: protein motif:HMM:NF025341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HXA09603.1"
                     /translation="MRAEINGKPALLGVDTGAPVSAIAASRRTHFGMSAIPGTSKLPL
                     RLKINGGFNRVTIAHRLRLGALTLIDEPMVAIDLRGPAHAAHDFHEQELDGILGADIL
                     FPTQAVLDCKQQVLFMKVDPDVPGTVPGVNHRGWKNMPIRVTKGWNLYVDGQLNGKAA
                     QLMIDTGAFTTLIHRPFVREMHLAVRDTPYTSGAVNLEERGLQLATIRRFSVGPFLVK
                     GKEVGVMDLQGLIHGDLLKGAPPVAGLLGSEFLRRNHAIIDFGTRTLYLKL"
     gene            complement(14208..14354)
                     /locus_tag="VNW28_06450"
     CDS             complement(14208..14354)
                     /locus_tag="VNW28_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09604.1"
                     /translation="MRNVWEWVRHGWPVALAYVIAFFAMLSVRAWQPDVPHKERAIEI
                     HASR"
     gene            14614..>15953
                     /locus_tag="VNW28_06455"
     CDS             14614..>15953
                     /locus_tag="VNW28_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXA09605.1"
                     /translation="MSQPTATNKVKLVQGLGLLDSTMLVAGSMIGSGIFIVSADITRL
                     VGSPGWLLVVWAVTGALTITAALSYGELAAMMPRAGGQYIYLREAYGPLWGFLYGWTL
                     FLVIQTGTIAAVGVAFARFLGVFASIISPTQWVVPPIILSTNYAVSLSTQQLVAILMI
                     VFLTFVNTRGLEVGKLIQNVFTSAKTLSLLALIVLGLFIGRNASALSANFANFWMPHA
                     VSPIKSDLSFVASVSATGGALGLLIAICVAQVGSLFSADAWNNVTFTAGEVRNPRRNL
                     PLSLALGTGLVITLYLLANLAYLCVLPLDKIQHAPDDRVATAAMSVMFGGAGSAIMAV
                     AIMVSTFGCNNGLILAGARVYYAMAQDGLFFKSTGRLNARHVPAVALILQGVWTALLV
                     LPRTRLRDPMTGAPLLDASGVEQYGNLYGALLDYVVFAVLIFYVLTIAALFVLRR"
BASE COUNT         3171 a         4740 c         4835 g         3207 t
ORIGIN      
        1 cgtttttgcg cggcctgctc catgtcggcg accgcagcca ggcgggaggg cgaatggcgg
       61 atagttcttc gccactgagc gacaatctgc gtgagttggg ttttaggacc gggcggttca
      121 agactggaac tccttgccgc ataaacgctc gctccatcga tttcgcagct tgcagcaaac
      181 agttgggaga cgagcccccc agccggttta gctatcttcc tgtcgacgaa gaattcgagg
      241 atgacgaaat ttcggccttg aacagagtcc gcaacggaag tttccacgtg gaacaaatgc
      301 catgctggat cacagcgaca actccggaaa cccacgagat tattcggcgg aatttgcatc
      361 gctcaccgct ctacagcggg cgcatcgagg gcaccggccc aagatattgt ccctcgatcg
      421 aggacaaggt agtaaaattt tcggacaagc cgcagcatca gctcttcctg gagcccgaag
      481 gtcgacaaac ccaggagttc tatattaatg gggtttccac cagtctcccc tatgaggtgc
      541 agatagaatt catccggtcg attccggggc ttcaaaacgc acagatcatg cggcccggtt
      601 acgcggtcga gtacgactat tttcctccca ctcaactcca atccacgctg gagacgaaat
      661 tggtctcccg actctatttc gccggccaga taaacggcac ctcaggttat gaagaagccg
      721 ctgcccaagg cttgatcgcc ggcgccaatg cggctctaga gctcgcaggt cagccggctc
      781 ttgttctcga cagaagtgag gcctatatcg gcgtcctgat cgatgatctg gtcacccgtg
      841 gcacgcaaga gccgtatcga atgttcacca gccgagccga acaccgcctt ctcttgcgac
      901 aggacaatgc cgaccagcga ttgaccgcca aagccgcctc cctgggtctc gtcggacacc
      961 ggcgaatgga aatactcgag aagaagtcgc gtctgttgca aaaggcccgc gagatcgctg
     1021 caacgacccg attggaaggc cagaccctcg ggcactccat gaaacacccg gaatttcatg
     1081 tacaaacgct gccgcggggg ctactgcgcg aagtcgacgc cgaaatttgg gagctcttag
     1141 agaccgaact gaagtacgag ggttatatcc gtcgccagaa cgagcaattg cgggcgacgc
     1201 acctggcgga gtcggttcgg atccccgccc agatcgatta ctcggtggtt cctgggttac
     1261 gaaatgaagc ccgccagaag ttggcgggcc ttcgtcccga aagcatcggt caagccgggc
     1321 gcatcagtgg cgttacgccg agcgacttgg gaattctgac aatctggctg agtaaacaga
     1381 acagaacatg tttacaatca acatcttaat tggtgcatta cccgattctt agctatctta
     1441 tcgccgttgc cattggatac cttctgggct cttgccccaa cggatatctc gtttcccgca
     1501 cccgcggaat cgacatcaga aaacagggca gcgggaacat tggggcgacc aacgtactgc
     1561 gagtcctggg aaagaaatgg ggttacctgg tattcgcctt ggacaccctg aaaggatttg
     1621 tggctgtcag gcttgcgttt gcagtcgttc tggcccttac ccctcatacg acgcagcgag
     1681 agctgatcgg tattgcgggt gggcttgcct gtattctcgg ccacaccttt cccatttggc
     1741 ttcggttccg gggcgggaaa ggcgttgcga cctcagccgg cgtgctgctc gggctgatgc
     1801 ccatcgccgt cttctcagtg tttgcggtct ggctgattct ttttcaagtc acgcgttacg
     1861 tttccgtggc gtccatcacg gcggccgccg cgctgccggt cttcgtcatg ctttacctgc
     1921 acctcaaatt gctgacgggc gcgagtctgc tgccgttttc gatcctgatc gcgggcgtcg
     1981 tcatctggcg acatcgttcc aatatgcgac gtctttttca cggaaaagag cagcgtttcg
     2041 gctaacactg gcgcgatggg aggaatagcc attctgggaa cgggcggttg gggcacggcg
     2101 ctcgccgtcc tctgggcctc gcacggcaac gacatcattc tttggggtca taccccggaa
     2161 cgggtgaagg agatctcgcg cagccggcaa aacccggatt acctgcccgc ggtggaattg
     2221 ccgcctaacg tttgcctaac gagtagtctg gccgattgca cggcggctga tttgatcgtc
     2281 tttgtcacgc cctcggtcgc gctgcgagag gtcgcggcga aactccgcga ggaattgacc
     2341 agtgaatctg ccatcctgat gagctgcacc aaagggatcg agcatggcac cggcatgcgc
     2401 atgagcgaga tcctgtcgga aaggttcccg caaaatcctg tgtccgtcct ctccggacca
     2461 aacctcgccg tcgaagtggc ccgcggatta ccgagcgcga cagttctcgg ttgtcgcgac
     2521 gaaacggtcg cggaacaact tcagtcgacc ctgggaagtt ctcgtttccg cgtttacacg
     2581 agcgatgaaa tcgtcgggat cgagctgggc ggcgccttga aaaacatttt cgccatcccg
     2641 gcgggagtga gcgacggctt cggcttcggc gacaattcca aggccgccct cttgactcgc
     2701 tccctggccg agctctttcg tctcggcgtc gcgatgggag gcgatgcccg cactttccaa
     2761 gggctgagcg gcgcgggcga tttgattgcg acctgtttca gccgtcacag ccgtaatcgg
     2821 ggactcggcg aacagctcgg acgtggcaag agcctcgccg agatcacgcg cgggatgaaa
     2881 acggtggccg aaggaattcc caccgcccgc agcgcctttg aatgcgcgcg caaactcggc
     2941 gtggaaacgc cgatcatcga ccaggtttat gcggtcctgc acgagggcaa gtcgcccgcg
     3001 caagccatgc aggagttgtt aggccgggat caaaaatccg agcggggcta gcttttcgcg
     3061 gccgccatct tcgccgccag atctgcgatc acggcgggga aaagttcccg ctctgcgagt
     3121 tggatccgct cgtgcagagt cgccggcgtg tcgttaggca aaatcggcac ttcccgttgc
     3181 gcgatgattt ctccgctgtc caccccagcg tcgacataat ggacggtgca acccgttatc
     3241 ttttcgcctg ccgataaagc ctgggtccac gcttcgcgtc ccggaaattt tgggagcagt
     3301 gaaggatgca gattgatgat gcggcctgga aacgcctcca gcaccgtcga cttgaggacg
     3361 cgcatgaacc cggcgagaac gatcagctcc accccggcgc cgcgcactag cctaacgagc
     3421 tgttgctcca cttccggttc gagcttcgtc cgaaattttc ccggcggcag aaaaacatcc
     3481 ggcacgccaa attcacgtgc cgccgccagg ataccggcat cggccacatc cgagacgacg
     3541 atccggcaat cggccgccag ctcgccgagt cgaatttttt ccaggatcgc gcggcagttc
     3601 gaacctttgc cggaaccgag gatcccgaga gcgagccgac tcatgcgctc ggcgggtgca
     3661 tccgggcgat cagaccggag gtggatttac ccgggacgag cggcagcgtc ttgatttcga
     3721 cccccgcctc ctggagcgcg ccgacctctt ccgcgttcag gctttccaga gtgtagtcac
     3781 cgcctttgac atagatcgcg ggtcgcgtgg ctttgatgac ttgggtggcg cgcaagtctg
     3841 ggaaaatcgt cacaaaatcg acgcactcca acgccgccaa tacttccgcc cgatcctggg
     3901 cggaattgag cggacgtccc gcgcccttca acgctcgcac cgactcatcg ccattgaccg
     3961 ctacggccaa aaagtcgccg agttcccgcg cctgtttcaa ataacgcaca tgcccgacgt
     4021 gcaagaggtc gaagcagccg ttggtcagga ccagccgttg ccttgcctga cgaacttcgt
     4081 ccgcgcgccg actcagctcc gagagtgaaa ggatcttcgt tttcaaatcg cctgccgaaa
     4141 gaatggcaga gcgcgccgcg taaatcaaag ctcccccaga gaacgcgagc tgggagcacg
     4201 ccccggctag gcggcctgcg gcttatagat cgcgcccact aatgcgccca tgatcgcgaa
     4261 gccaatcaaa ctgccgccga tccacatcca aagaatgtat gttgtcaaag gctccaccgc
     4321 gaaacgcacg aggtcgatac ctgtgcaaaa aactccgaat acgatgccgt agcccaagcc
     4381 ggtcgccatg ctttggacgc cgaccttgct gacgtacagg ccggtgaagg caaaggccat
     4441 caccgcgtgt ccgagcagaa gaatccaaaa gtggctattg aaatccggat ccgcccgcca
     4501 gagcgcggcc gtttccgtat aagcgctctt catcagaatg ccgtgccaga cgaacccgaa
     4561 gaagaagata aaaacaaacg caacaatcaa cgcgataacg cctcgtttat gattcatggg
     4621 cctgtcatac tgagcgtcac gtcccgttgt cgatccaaac cgaaatggcg aaccgcgcta
     4681 agttcgcgtt ttgccaaagc gaatccagca aggaacgcga agaagaatcg ctttgtgttg
     4741 aagcgaaacg gccatcgagt tccgtttggc catgtcgatg caaagcaagc ccggcgagcg
     4801 caggcgcttg gaagtcccgg cccttcgcta ggggcgcgat ccgttcggtg taatcgaagc
     4861 cccagcgcga ggttttcttc acccaagaaa cgctttacgc ttgtcgatgc tttcacctaa
     4921 ccggctggct atagcgattg tcgcgcttct cgttctcgat tttccactcc aggctcaaat
     4981 tgatcggatc accggaaaga atttcgccac ccgctcggaa gtgctggcgc gacacggaat
     5041 ggtttgcacg agcgtgccgc tcgccacgca agttggcctc gacattctga aacgcggggg
     5101 cagcgcggtc gatgccgcga ttgcagcaaa cgcgacactc ggtttgatgg agccggtttc
     5161 gaatggcgtc ggcggcgatc tcttcgccat cgtttacagc gcgagggaaa acaaacttta
     5221 cggaatcaac gggagcggcc gttcgcccct cggcttgagc tatgaccaga tgaaagccga
     5281 gcttgcgaaa ttgaagcgcg agacgatccc gcccaccggc atgctgccaa tcagcgtgcc
     5341 gggttgcgtc gacgcgtggt cggagctgca caggaaattc gggaatctga aactcggcga
     5401 cgatctcgcg ccggccatcc agtacgcgga agaaggtttt cccgtaaccg aattgatcgc
     5461 gtattactgg ggtttcggtc cgcacatcta taaagggttg cccggcgctt ttcttgaaac
     5521 ctacacgctc gatggcaaag gtcgcacccc cgccaaaggc gacatcttca agaatccggc
     5581 gctggcaaat acgttgcggc tgatcggcga gaaaggtcgc gacgctttct acaaaggcga
     5641 gatcgcggac aagatcgacg cgttcatgca agcgaacggc ggttacctgc ggaaggccga
     5701 tttcgaaaga cacaccagca cgtgggtcga tcccgtctcg acgaattacc gcggctacga
     5761 cgtcttcgaa ttgccaccga atggccaggg catcgccacc ttgcaaatcc tgaacattct
     5821 cgaaggcttc gacctaaagg cgatggggcg taattcgccc gagacattgc acgccatgat
     5881 cgaagcgaag aagatcgtct gggccgatcg ggccaggttt tacgctgatc ccgcctttgc
     5941 aaaaattcca ctcgccggct tgctctcgaa aaattacgcg gctgagcggc gcaaattgat
     6001 cgacccaaat cacgccgcta aaaaagttgg ggccggcaac ccccccacgc tcgatcaggg
     6061 cgataccatt tacctgtgca ccgccgatca cgaaggcaac atggtctcgc ttattcaaag
     6121 caactatcgc ggccagggca gcggcatcgt cgtgcccggg ctcgggttca tgtttcagga
     6181 tcgcggcgaa cttttctcga tggagcccgg ccacgccaac gcttacgggc cgggcaaacg
     6241 cccgtttcat accatcatcc ccgggttcgt catgaaagac ggcaaaccgt gggaggcgtt
     6301 cggagtcatg ggcggcggca tgcaaccgca agggcacgtg caagtgctca ctaaccagat
     6361 cgacttcggc ttaaacgtgc aggaagctgg cgacgcctct cgttggcaac acgaaggcga
     6421 caacgaaccg accggcgaaa agatggagaa cggcggctac gtcgagctcg agagcggcat
     6481 tccctacgaa accgcgcgtg aactgcgcaa gcgcggccac gacgtgcgcc tcgatgtcgg
     6541 cggctacggc ggttaccagg cgatcaaagt agaagtacac gatggccaac gcgtttacgt
     6601 cggcgcgagc gagtcacgca aggacggaca agccgcgggt tactgagata gtccggccgc
     6661 atgatgcact cgcctagctc ggatcgccga agaaacgagg aagcgtaaag tgcagatctc
     6721 tcagaagcgg caacgcggcc gctatgttga tcgctcggat tcgttgttcc gccaattggc
     6781 cgaaatacaa ctaaggcaaa cgaccacgga gcaagtggcc cgtgactacc gagggcctct
     6841 tttagtgcca agcggaaagc ggcctggccc taagttgcag ctctcttgcc actttcattt
     6901 gtggatcgca tgttgcttgg atcgttagac catggccaga caattcaaac cagtgcggtt
     6961 cttcgtgatg atggcagcgg tggcatttat cgtctgtggc gtgacttcgt tctttaccca
     7021 tcgtgcggag catgggagga cgagtgagga gcggtctgct tatttcatcg gagagaaggc
     7081 gggagaggag gctgcgccag gaggacagct gcccaccgcc gccgccttga acataatggc
     7141 tcagcaatct ttcgagcggc agggctcggg cgaaaaggaa aattgggatc tcgccttcga
     7201 acacggttac gaagacggat ttaagaagac gcactccgca ccgtgagcaa tctggcaggc
     7261 ctataagtcg gcagcgactg gagcccagat tcatctcgtt cccgcaggcg tcttacactt
     7321 gcgcaggatt gggccgcagg cactttggcc gatgcaaaga attactccat ttttgtggtt
     7381 cgacgatcag gctgaggagg cggcgagttt ctatgtctcc gtgtttaaga attcgaaggt
     7441 caaagcgatg actcattaca ccggagagga gccttcggga cagaagggct cggtgatgac
     7501 ggtggacttc gagttggatg gacaggattt cgtggccttg aacggcggcc cgcgcttcaa
     7561 gttcaccgaa gcggtctcgt tcgtgatcga ctgcgagacg caggaggaga tcgattatta
     7621 ctgggagaaa ctcactgccg acggcgggga agaggtgcaa tgcggctggc tcacggataa
     7681 atacggtctc tcctggcagg ttgtgccgaa caagtgggcc gagtgggcca ggaatgcggc
     7741 cgggatgcaa cggctgatgc atgccttgat gccgatgaag aagctcgata tcgccgcgct
     7801 ccagaaggcc tacgacggga agtaacgccg gaacttcggg acgtcttgct tatgcaaatg
     7861 ccggcgagct gcggcctgtt cgttaggctt cggaaaaaaa agcggtcagc ggactggtcg
     7921 ccgaggcggt cgtgcgggtt tccgccaaac cgatttcgcg cgcgtcgcgg ccggcttcga
     7981 cggccttgcg gaaggcgtgc gccatgcgca ccggatcgga cgcgatcgcg atcgcggtat
     8041 tcacgagcac ggcatcggcg cccatttcga gcgcttcggc cgcctgactc ggggcgccca
     8101 agccggcgtc gaccaccacg ggcacggtcg cctgttcgat gatgatctcg atttgcgcgc
     8161 gcgtttcgat tccgcggtta ctgccgatcg gtgaaccgag cggcatgacg gttgcggtcc
     8221 caacatcctg caggcgtttg gccaggaccg ggtcggcgtt gatgtaggga agaacggtga
     8281 agccctcctt caccagaatc gtcgccgcgg cgagggtctc gaccgggtcg ggcagcaaat
     8341 aacgcggatc gggatgaatc tcgagcttga tccacgacgg cagacccgcc gtgaccgcga
     8401 gacgcgccag cctgacggct tcatcggcat tgcgcgcgcc gctcgtattc ggcagaagga
     8461 ggaaacgttt cgggtcgatg aaatcgagaa tgttagcgaa cgggtcgccc ttgcccgaga
     8521 gatcggcgcg gcgcagcgcg accgtgacca gctcggtgcc gctcgcgtcg agcgcgtcgc
     8581 gcatcagttc gttggaagcg aacttgccgg tgccgacgag caggcgcgaa gtaaattcgc
     8641 gatctgcgat tttgagtttc gacggtttgt ccgacattcg cttaatctat agcgcacaat
     8701 cttcctgtca tcctgagcga gcgcaccgga gtcgaaggat cccatggcgg attcaggaag
     8761 cgggggttgc agggagcccg gcgggatcta ctcggcgccg ctcggaatga cttgtactcg
     8821 ccgtcacatc tgtttagctt acgcggtgac ccggttcgat ttgcggcttg gcgattgcgt
     8881 agcgggaatg gcccaattgg agactgcttc gatcgatctc gtggttactt ctccgcccta
     8941 caatctcggg attcactatg ggaagttcgc cgataccgcg gatcgcgaaa cgtatctcga
     9001 ttggtgcgaa caatgggccg cgcagatccg ccggctactc cagcctaacg gatcgctttt
     9061 cctcaacgtc ggctcagcgc cctccaatcc gatgctgcca cacgagctga ttttgcgact
     9121 gcgcaaaatt ttcgtcctgc aaaacacgat ccattggatc aaggcgatca caattccaga
     9181 cgagactggt gaagaaatct cgcgcggcca tttcaagcca atcagttcgc cgcgcttcct
     9241 gaacgactgc cacgaataca tctttcatct caccccaaac ggaaaaacgc cgctcgaccg
     9301 tcttgcgctg ggtgttccct acgccgacaa atcgaacatc gcgcgctgga gccacacccg
     9361 cggccgcgac cggcgttgcc ggggcaacac ctggttcatt ccctacgaaa cgatccaacg
     9421 tcgggccaaa gaacgcccgc atccggcgac ctttccggtc caactggcga cgaattgcat
     9481 tcggcttcac ggcctcacga gaacgcagtc gatgctcgat ccgtttctcg ggattggaaa
     9541 ttccgcggcc gcggcgcgcc gcgccggcct aacgaatttc gtcgggttcg agatcgacga
     9601 ggaatatctg gccgaggcga ggcggcggat cgggtagcgc gcacgtctcg cgtgctggtc
     9661 gcggtgtttc accgcgacag gcctttgagt gcgcggctgc gcttgaaaaa ccagcaaggc
     9721 tttcaggcgc ggggagaaaa gttcatgacg gcgggacgcc gtcaccagca cacgggaggt
     9781 gcgcgctacc cggaaaattt cgccagcggt gtgatcagat tcccaggcag cggccgcacc
     9841 cgctcgaaaa tgcgaccagc gagcggcttc gccaccgcgt aaccggcgaa gatcatctca
     9901 aggcgcgcgt ccagattgtc gatgtaattg agggcgagcg cttcgggcgt tttcggataa
     9961 accggcgacc cgaactgcgg ttcgccgtgg tgcgaggcga tgagatgcag taaatgcagc
    10021 cggcaatcct ccgtcggcgg cgagagtttt tgccattccg ccgcgcgccc gtcggccttg
    10081 atcttttgcc aaagcgaatt gaccacctcg agcccgatgc cgatgtggcc gaccatttcg
    10141 cccagctcat cgaaaggcat gctgaagccg gtctcgggca ggtagttttc ccaaagcttg
    10201 ccggcgtcgt gaaagagaat cccggcgacg agcaggtcga gattcaagcc cggatattgc
    10261 ggcgcaatct ccgtcgcgac gcgcatcatc tgcgcggtgt gctcgaccaa accgccacgc
    10321 cgcgcgtggt gataggtgcg cgcaccagcg gtgttgcgaa agcgcgcacc gtattgcgcg
    10381 atgaattcgg cgcagagcga gtagagccgc gggtcgcgga tcgccgcggt cgtcgcgacg
    10441 ataaattccc aatcggccgc ctgcttcgcg cgcagctcgg ccggaccttg caaaaggtgc
    10501 gctcgttcct cggcggtgag cgggcgcgat ttccactggc gggcatccag gccgtattgt
    10561 tgatgctggt aaaattctcc tgtgatctcg acgaaatcgc cgctctgcat cgcgtcgcag
    10621 gctttgaaat tcggactgtc gctccagacg cgcagcgtca tgcgatcggc cgcatcggcc
    10681 agcgtgagct cgcaatacgg cttctgctcc cgggtaaatt tcgccgcgac cgtctcgagc
    10741 tgcacgtgga cgcgtgcctc gagcgcgcct tgcgggacgg cctggcggat ttccttgagg
    10801 gtcagcaatt gcatcggagg cattcttggc atcgggctgc gccttggaac agccgaaagt
    10861 gggaggggcc ttggcgcccc gatctgcgcc gcgcaaagag ggctcccacg ttagggactc
    10921 cgatcgttag ggcttggcga tcccgatcac gccgcaggcg acgcgggcgc cggcattgcc
    10981 gaccggctgc gatttcaagt cgtcctcttt ctcatgcacg ataacgccgc gaccgatgat
    11041 cgatttctcg ccgctcatcg tcatcatgtt gtcggtcagc tcgagatgcg ctttgcccgc
    11101 gctgtcggct tcgaggttgc ccatgtcgcc ttcgtggcgt tgcgcggcgt catggccggc
    11161 gtgcgggctg ttcgtcgggt tgaagtggcc gccggccgct ttcccatccg gcgacgagca
    11221 atcgccgaac tcgtgaatgt ggaacccgtg tttgcccggc gtcagtccgg tgacatcggc
    11281 gacgattttt atttcatcgc ctgatttggt gaacgtgaca gtgccttcca cgttgctgcc
    11341 cttggtggga tgcaagacgg cgatcgcctt ggtgatctca ccggcgaacg cggtcgccgt
    11401 gagcagagag agtgagcctg ccgcgagcag gcagatcgct gcgcgaagtt ggtttgtttt
    11461 catcggtcag actacgacga gtgcgacgaa acaaaagtcg acgcgctcgg gactcgtgat
    11521 tcaatccagc aaccgttgtt cgagacccgc gtaggcatcg atgcggcgat cgcgcaggaa
    11581 tggccaatgg gtacggtgtt cgttgacgcg cttccattcg acatcggcga agatgatttc
    11641 ttcgcgatcg acgctcgcct tggcgaggat ctcgccgctc ggcgcgacga tgaagctttg
    11701 cccccagaac tcgatcccgt cgccgccggc gggcgcttcg tggtcgacgc gattgaccgc
    11761 cgcgacgaag cagccattcg cgatcgcgtg gctgcgctgc atcgtttccc aggcggaatg
    11821 ctgggcgtag ccgtacttcg ctttctcggc gggatgccag ccgatcgcgg tcggataaaa
    11881 gagaatctcg gcgccgcgca gagccgttag gcgagcggcc tccggatacc actgatccca
    11941 gcaaatgcag acgccgatcc tgccctgcgg cgtcggccag gcctggaaac cgagatcgcc
    12001 cggcgtgaaa taaaatttct cgtaatagga aggatcatcc gggatgtgca tcttgcgata
    12061 cttcccgagc agttcgccgt gaccgccgat gatcgcggcg gtgttgtgaa agagaccggc
    12121 ggcgcgcctc tcgaagagcg acgcgatgat gatcgcgcca gtctcgcgcg cgacttcgcc
    12181 taacgactgg gtggacggcc ccgggatggg ctcggcgagc gcgaagttgg cgtggtcctc
    12241 cgattggcag aaatattgcg accggaaaag ctccggcaga caaataatct gcgcgccctg
    12301 ccgggccgct tcgcgcaccc gtgcgacggc cttggcgaaa ttggcctccg gctcggggcc
    12361 gcactgcatc tggatcagtg caacccgcga gctgtcgttg ggcattctac ttgaccgaag
    12421 ccgccggcgc gatcttcacg ctctcgagcg cgacgcgagt ggtcggcttg ctcgcttcgc
    12481 ccatcgcgct ccgggtgacg ggcgtatcgc cgatcttatc gagcacgtcg aggcctttga
    12541 tcactttacc gaaggcagtg tattgatggt cgagcgagct ggccgtgccg agacagatga
    12601 aaaactggct cccggccgag ttcgggtcgg cggaacgcgc catcgagaga acgccgcgga
    12661 catgcggatg atcgttaaat tccgccggga ttttatagcc cgggccgccg gtgccgtagc
    12721 tgctttcgtt cgccagatcc ttcgtgttgg gatcgccgcc ctggatcatg aatcccttga
    12781 taatgcgatg aaatgcggtc ccatcgtaaa agccctggcg cgccagtttc ttgaaattct
    12841 cgaccgtctt gggggccacc tcgggccaaa attcgacgac catttcgcca tcgctcgtct
    12901 tgagcacggc cacttccttt tgatttgtat ccatgggttt ctttgcttcg ggtttggttg
    12961 cgtcctggcc gcgaacatgc gccaggccaa gagagcagac gagcaggcag cagaacaaag
    13021 tgagtttcat ttcagacctg tggcatggat ggcgcggggt tgcattctat ttttcaggct
    13081 tcgtccttca tcttgctcct aatctcgtgg actcgagcag gagcaggatt aggagcagga
    13141 aggagaggga cgtgtttaca gcttcaaata gagggtgcgc gtgccgaaat cgatgatcgc
    13201 gtgattgcgg cgcagaaact ccgagcctaa caatcccgcg accggcggcg cgcctttcag
    13261 caaatcgccg tggatcaacc cctggaggtc catcacgccg acctcttttc ctttgaccag
    13321 aaaagggccg accgaaaagc gccggatggt cgccagttgc aagccgcgct cttcgagatt
    13381 gaccgcaccc gaggtgtaag gcgtgtcgcg caccgcaaga tgcatctcgc ggacgaacgg
    13441 ccgatggatg agggtggtga aagcgccggt atcgatcatg agttgggccg cttttccgtt
    13501 tagctggccg tcgacgtaga gattccagcc tttggtcacg cggatcggca tgtttttcca
    13561 gccgcgatga ttgactcccg ggacggtgcc gggcacgtcg ggatcgactt tcatgaacaa
    13621 gacctgctgc ttgcaatcga gcaccgcctg ggtcgggaac aggatgtcag cgccgaggat
    13681 gccgtcgagt tcctgctcgt gaaaatcatg ggccgcgtgg gccgggccgc ggaggtcgat
    13741 cgcgaccatc ggctcgtcga taagagtcag tgcaccgagt cgcagccggt gcgcgatcgt
    13801 gacacgattg aagccgccgt tgattttcaa acgaagcgga agtttggaag tgccgggaat
    13861 ggccgacatt ccgaaatggg tgcgacgcga ggctgcgatc gcgctgaccg gcgcacccgt
    13921 atccactcca agcagggccg gcttgccgtt gatctcggcc cgcaccaaaa ggtgattttg
    13981 gctcgaccgc ccgagaggca aagcctggaa gtctggcctc tccaaggctg gctgcggttg
    14041 cgaccggtca gcgccaaaag ccggagctac gccgagcagg accgcgaagg ccgagagagc
    14101 cgcggcccgc gttaaccatg atcttgcggc gtttgaccgg agttcgagaa tcttcacgcg
    14161 cccgatggta gcacgtctcg ccacgaaggc gaagtcagtt gctccagcta gcgggacgca
    14221 tggatttcga tggctctttc cttgtgcggg acgtcgggct gccacgcccg aacggagagc
    14281 atggcaaaaa aggcgatgac gtaggcgagg gcgacaggcc agccgtgccg cacccattcc
    14341 cagacatttc tcatcgtgct aaacgattcg cagtggcaac gccgcagtcc ggcgagcgtg
    14401 caggaaaggc gacgtcaaga tcaagtgccg cggccggtat gtcagcgatt ttgcgagccg
    14461 ccatgccagc gcatcggccc tcggcgaaac cgcttgcttc gcccctcgtc aaccggtatg
    14521 ccgggcggga gaaatcttat gccggagcgc ctttttcgtg ttgtccccta attcgttagg
    14581 cgaacatcgc ggcatttggc caactcaaac tccatgtcac agccaaccgc caccaacaag
    14641 gtaaagctcg ttcagggact cggcttgctc gactcgacga tgctcgtggc gggctcgatg
    14701 attggctcgg gtattttcat cgtctcggcc gacatcacgc gcctggtcgg ctctccgggc
    14761 tggctcctcg tggtgtgggc ggtgaccggc gcgctcacaa tcacggccgc actgagctac
    14821 ggtgagctgg cagcgatgat gccccgggct ggcgggcaat acatttatct gcgcgaagcc
    14881 tacggtccgc tctggggatt cctttacggc tggacgctgt ttctcgttat tcagactggc
    14941 acgatcgccg cggtgggggt ggcgttcgcg cggtttctcg gtgtcttcgc gtcgatcatt
    15001 tcgccgacgc aatgggtcgt cccgccgatc attctctcga ccaattatgc cgtcagtctt
    15061 tcgacgcagc aattggtggc gatcctgatg atcgtgttcc tgacgtttgt gaacacccgc
    15121 ggcctcgagg tcggcaagct catccaaaac gtcttcacct ccgccaagac gctttcgctc
    15181 ctggcgctca tcgttctcgg tctcttcatc ggacgaaacg ccagcgcact cagcgccaac
    15241 ttcgccaatt tttggatgcc gcacgcggta tcgccgatca aatccgacct atcgttcgtg
    15301 gcctcggtct cggcgacggg cggcgcgctc ggccttttga ttgccatctg tgtcgcgcag
    15361 gtcggctcgc tcttttccgc ggacgcctgg aacaacgtca cgtttaccgc cggtgaagtg
    15421 agaaacccgc gtcgcaacct gccgttgtca cttgcgctgg gcactggact ggtcatcacg
    15481 ctctacctgc tggccaatct ggcttatctt tgcgtgctgc cgctggacaa gatccagcat
    15541 gcgccagacg accgcgtcgc caccgctgcg atgtcggtga tgttcggcgg cgcgggctcg
    15601 gcgatcatgg cggtcgccat catggtctcg acgttcggct gcaacaacgg actgattttg
    15661 gccggcgcgc gggtctatta cgcaatggcc caggacggat tgttctttaa atccaccggc
    15721 cggctcaacg ccaggcacgt tcccgcagtt gcgctgatcc tgcaaggtgt gtggacggcg
    15781 ttgctcgtgc tgccgcgcac gcgattgcgc gatccgatga caggcgcacc gctgctcgat
    15841 gcctccggtg tcgagcaata cggcaacctc tacggcgcgc tgctcgatta cgtagtcttc
    15901 gccgtgctca tcttttatgt gctgacgatt gccgctttgt ttgtcctgcg gcg
//
LOCUS       DATMNS010000189         2524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4915724, whole genome shotgun sequence.
ACCESSION   DATMNS010000189 DATMNS010000000
VERSION     DATMNS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4915724"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..432
                     /locus_tag="VNW28_06460"
     CDS             88..432
                     /locus_tag="VNW28_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HXA09606.1"
                     /translation="MEHERFEGERTLMRIYIGESDKWHGKPLYEALVEFFRKEGLSGA
                     TVLRGVGGYGSTSRYHTEKILRLSQDLPLVVEVVEDSERVERVLPKLDEMIGGGLITL
                     EKVRVILYRAHK"
     gene            510..1580
                     /locus_tag="VNW28_06465"
     CDS             510..1580
                     /locus_tag="VNW28_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09607.1"
                     /translation="MKTRTVLDRTARFALFLLAPVVILAAGGYHVTHRLPILGDTGWD
                     YITADSQARRLYIPHGTEVVLLDLTNYRSSGKIDGLKGVHGVALAREFGHGFISATDP
                     GSVTMFDLKTLAVLNKERVGDDPNGIIYDPFTKRIFSADRGSQRLTAIDAKTGKVVGA
                     VDNLGGRTEHLASDEAGHVFLNMQDVGKLQKIDAQNLKVLETWQIAPPCGQPSSMDID
                     RAHNRVFIGCRGGGSLQPPAPALLAVVDASNGKVVATHPIGAGVDALEFDPQRGLIDV
                     STGGGDGALSIFHQDGPDQYSLVQTVRTLPGARTMALDHKTGKVYLPVADFGPAPEPT
                     PDNPRPRPRMVPGTFSVLVVAK"
     gene            complement(1585..2283)
                     /locus_tag="VNW28_06470"
     CDS             complement(1585..2283)
                     /locus_tag="VNW28_06470"
                     /inference="COORDINATES: protein motif:HMM:NF020922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 family protein"
                     /protein_id="HXA09608.1"
                     /translation="MAANRRAPTHDNGRISAETFRFFRDLSRNNRKEWMDENRERYKS
                     AIVQPFRGLLEELTPAVTKLFPGIDVSGRTGVNFSRINRDIRFAKDKTPYHPRMYLLF
                     PARGGKNRVPGELYLGVAAGIVTAGFRVYFDRNTKAAALASRIGQGPKWCAQQKRRTA
                     RRYESYWYSMEKGEWTKNDGWPLTPEDWKKLRAWVVRRKLSTAAAARPNFPEQVAGIF
                     RQVFPLFRFVCHCT"
     gene            complement(2290..>2524)
                     /locus_tag="VNW28_06475"
     CDS             complement(2290..>2524)
                     /locus_tag="VNW28_06475"
                     /inference="COORDINATES: protein motif:HMM:NF013199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09609.1"
                     /translation="TGEFVALDAETGKTLWQFKTGSSVNSTAITYTHNGRQYVSIASG
                     LGGALATRYGAAKVPAGGSIWTFALFPETSSKR"
BASE COUNT          516 a          765 c          728 g          515 t
ORIGIN      
        1 ggagcaagcg tgctgggcgg gctgatcgcc atgatgctgg gaatgcgagt gggcgacgcc
       61 ctctgagtcc cttccacgga ggactcaatg gaacacgaac gatttgaagg cgagcgcacg
      121 ctgatgcgca tctacattgg cgaatccgat aagtggcacg gcaagccgct ttacgaagcg
      181 ctcgtcgaat ttttccgcaa ggaaggactg agcggcgcga ccgtactgcg cggcgttggc
      241 ggctacggct caacaagccg ctatcacacc gagaaaattc tgcggctctc gcaggatttg
      301 ccgctagtcg tcgaagttgt cgaggattcc gagcgcgtcg agcgcgttct tcccaagctc
      361 gatgaaatga ttggcggcgg cctgattacg ctggagaagg tccgcgtaat cctctatcgc
      421 gcccacaaat agcgtgacgg tccgagagtt gtcattctga ggagcgcagc gacgaagaat
      481 ctctcttaat cacaacggag aagcgcatca tgaaaacacg cacagtcctc gacagaaccg
      541 cacgcttcgc gctattcctg ctcgcgcctg tagtgatctt ggcggctggc ggctatcacg
      601 taacccatcg ccttccgatc ctcggcgaca ccggctggga ctacatcacc gccgatagcc
      661 aagcccgacg attatatatt ccacatggta ccgaagtggt tttgctcgac cttaccaact
      721 acaggagcag cggcaagatc gatggactga aaggtgtgca cggcgtcgcc ctggcccgcg
      781 aattcggcca cggtttcatc agcgccaccg atccgggttc ggtcacgatg tttgacctca
      841 aaactcttgc ggtgctcaac aaggaacgcg tcggagacga ccccaacggg atcatctacg
      901 atccattcac caagcgtatt ttcagcgccg atcgtggcag ccagcgcctc acggcaatcg
      961 atgccaagac gggcaaagtt gtcggcgccg tcgacaattt gggcggcaga accgagcatt
     1021 tggcttccga cgaagccggc cacgtcttcc tgaacatgca ggacgtcggc aagctccaga
     1081 agattgacgc ccagaatctg aaagtgctgg aaacgtggca gatcgcgccg ccatgcggtc
     1141 agcccagcag catggatatc gatcgggcgc acaatcgcgt cttcatcggc tgccggggcg
     1201 gcggttcgtt acagcctccg gcgcctgcac ttttagcagt tgtcgatgcg tcgaacggta
     1261 aggtcgtggc cactcatccg attggagcgg gcgtggatgc gttggaattc gacccccaaa
     1321 gaggtttgat cgatgtttcc acgggcggcg gagacggcgc gctctcgatt tttcaccaag
     1381 atggcccgga tcagtattcg ttggtgcaaa ctgtaaggac cctgccggga gcgcgcacca
     1441 tggctctgga tcacaaaaca ggcaaagtgt atttgccagt tgcagatttc ggccctgccc
     1501 cagaaccgac gcctgacaac ccgcgtcccc ggccgcgcat ggtccccgga acgttttccg
     1561 tcttggtcgt tgccaaatag aaaatcacgt gcagtgacaa acgaagcgga atagcggaaa
     1621 aacctggcgg aagatccccg cgacctgctc cggaaaattc ggtctcgccg ccgctgccgt
     1681 cgaaagcttt ctgcgcacaa cccaggctcg cagctttttc cagtcctcag gcgtgagcgg
     1741 ccagccgtca ttcttggtcc actcgccttt ctccatcgag taccagtagc tctcgtatcg
     1801 acgcgccgtc cggcgtttct gctgagcgca ccatttcggc ccttgcccaa tccgcgaagc
     1861 gagcgcggcg gcctttgtgt tgcggtcgaa gtaaacgcgg aatcccgccg tcacaatccc
     1921 agcggcgaca cccaggtaaa gttcccccgg cacgcggttc tttcctcctc gagcaggaaa
     1981 aagcaaatac atccgcgggt ggtagggtgt cttgtctttg gcgaaacgga tatcgcgatt
     2041 gatgcgtgag aaatttactc ccgtgcggcc gctgacgtcg atcccgggaa atagcttcgt
     2101 cacagcagga gtcaactctt cgagcagccc gcgaaacggt tggacgatgg cgcttttata
     2161 gcgctcccgg ttctcgtcca tccattcttt gcggttgttg cggctgagat cgcggaagaa
     2221 tcgaaacgtc tccgcgctaa tccggccgtt atcgtgagtt ggcgctcttc ggttcgccgc
     2281 catcgccctc taacgcttgc tgctcgtttc gggaaacagc gcgaacgtcc agatcgaccc
     2341 gccggccggt actttcgccg ctccatagcg tgtggcgaga gcgccgccga gccccgaggc
     2401 gatgctcaca tattggcgcc cgttatgcgt gtaggtgatc gccgtcgaat tcacgctcga
     2461 tccggtcttg aactgccaaa gcgttttgcc ggtctccgcg tcgagcgcaa cgaactcgcc
     2521 cgtc
//
LOCUS       DATMNS010000190         4368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4916289, whole genome shotgun sequence.
ACCESSION   DATMNS010000190 DATMNS010000000
VERSION     DATMNS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4916289"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /locus_tag="VNW28_06480"
     CDS             complement(<1..932)
                     /locus_tag="VNW28_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HXA09610.1"
                     /translation="MNPTPILPENDPPAGTPERADLRPESINDAVIRLAGDSQDGIQS
                     AGAFLARLAGRSAQDVMTYMTIPATISGGPSIFQVRIGTGEVLSAGDEADFLVAFYQH
                     SYQDHIGFLKEGGVLLYDSDNVEPNLDDKRFVYVGVPITGLTVEALGGTAKDKGKNIF
                     VLGLISKIFNLDVEKLTTLIKEKFAGKDASIANTAIMAFTAGYAYPVGNVLTKQYKFE
                     TVAPKEGDRAQITMDGNQALAYGLIAAGVRYGAGYPITPWSSVMEILRSELPKYGGIF
                     VQAEDELGAVSLALGFSFSGYLSVTGSAGPGISL"
     gene            1214..2434
                     /locus_tag="VNW28_06485"
     CDS             1214..2434
                     /locus_tag="VNW28_06485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HXA09611.1"
                     /translation="MGGLVVKPRARILHGHDWVFSGEVLKVFGNPADGDVISLRDGRD
                     RLLGSAIYNSKSQIVARRFSRRKQELDLDFFQRRIAQAIEYRARRGIEPRLCRLVWSE
                     SDGLPGVIIDRYGDCLVLQTLTLAMDQRKELIVRALETHFCRASAPLAEGKEAGGAPA
                     LQSIIERNDAPVRRAEGMELQSGLLFGVAPDEIEIEVAGLKLQIDLLHSQKTGFYLDQ
                     LPNYALVAHHAAGRRVLDCFASQGGFALACARAGASEVVAVEAGAASLRAAEQNAVRN
                     QLAVRWVEQDVFQFLRGAEKAEEEFDLIILDPPSFTKSKGGLHDALRGYKELHVRAFK
                     LLSPHGMLATFSCSHHVGAEIFEKMIADALVDARRSARRLARYEQALDHPVLPTLPET
                     EYFRGTLLEMMPGR"
     gene            complement(2444..4270)
                     /locus_tag="VNW28_06490"
     CDS             complement(2444..4270)
                     /locus_tag="VNW28_06490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXA09612.1"
                     /translation="MRPATGVVRSRERYGGILIGRAAIARIFTTDFRISLSQRCKLRP
                     WSIPRLQSKVFFENQALHSELISRPEETLYQRVNRLREQFRAQFCSSTFPQLLHRFCW
                     AGAILLAVLLRGPTICSGLPYIDYIDEGYVLHQSIELLNHRSLDTKWYYYPSLPAYLT
                     AAALIAYSPVYRLVHGHGFRKDLPRDRDIHTSKGDNYDLISPPALIVAGRFVAIFLSV
                     GTVVLAGRIAGILAGDRVERLSLILVATCPALTSRASNIIVDTFATFFAVWSLFFCAC
                     LVKEPAMGDGRMSRRAAYAGAAAGLAFASKYTVAVVFAAVAFTLITLPVSKTFRLRLL
                     GLASGGFCFTAALMAPATVSNSAAVFRALLALAHGYTTMTSVPGYFGQAVSNYELGWP
                     LMIVGCLGLVLMLRSEIDRPVALGWILFGVILIGLFIGKPFQPFRNLLPLLPPFCIAG
                     AFAFHQLLGWSGRSGQAALWNPVIALVIGAIVINAGFSSFEPLRYRIFHRDSRIQAVD
                     WLQARVRKTDRVLAIAELGLLPSELSRIGASVQVASWSDAVALLQRKEFDYLISGFST
                     PRPATSASFSPRASFGNVVTPTEPYIWRTNDEWIRIFSRRPD"
BASE COUNT          969 a         1266 c         1336 g          797 t
ORIGIN      
        1 ttgagggaga ttccggggcc ggcactcccg gtgacggaga gatatcccga gaaggagaag
       61 ccgagggcca aagacactgc gccgagctcg tcctcggcct gcacgaagat gccgccgtat
      121 ttcggaagct cgctccgcaa gatttccatg acggacgacc acggcgtgat gggataaccg
      181 gcgccataac gcacgcccgc ggcgatcaat ccgtaggcga gcgcctggtt gccatccatg
      241 gtgatctggg cgcgatcgcc ttccttggga gcgaccgtct caaacttgta ctgcttcgtc
      301 aggacgttgc cgacggggta ggcgtaaccg gccgtgaacg ccatgatggc ggtgttggcg
      361 atgctcgcat ccttgcccgc gaacttttct ttgatcagcg tcgtcagctt ctcgacgtcg
      421 agattaaaaa tcttcgagat caggccgagg acgaaaatgt tcttgccttt gtctttcgcc
      481 gttccgccga gcgcctcgac ggtgagtccg gtgatcggca cgccgacgta aacgaagcgt
      541 ttgtcatcaa ggttcggctc gacgttgtcg gaatcgtaaa gcaggacgcc gccctctttc
      601 aaaaagccga tgtggtcctg ataactgtgc tgatagaacg caaccaggaa gtcggcctca
      661 tcgcccgcgg aaaggacttc acccgtgccg atgcggacct ggaagatgga cggtccaccg
      721 gagatggtag ccgggatcgt catgtaggtc atgacgtcct gcgcgctgcg tccggccagg
      781 cgcgcgagga aagctcccgc gctttgaatg ccatcctgcg aatctcccgc gaggcggatg
      841 acggcgtcgt tgatgctctc ggggcgaaga tcggcgcgtt cgggcgtgcc ggcgggcgga
      901 tcgttttcgg gcaggatggg tgttggattc acgcagcgag tagagtggcc gttaaaatga
      961 caagtttagc cgtgtactct gaggcaatgc ggccgtaatt gccagtacga atacgaacgg
     1021 cgccgatgtt tcggctggga actgccgggt aaaaaccgtc gcagcgaccg ccgaacgtcg
     1081 tccggagtcg ccgggtgtcg aggtggggtc ggtttgtggc gcggtcttgg cctgcttttc
     1141 gcgccgcggg gcgacagccg ccatcagcga cttccgcttg ctctggaatt ccctctgccc
     1201 gtcagtgagc gcgatgggcg gcctcgttgt taaacctcgc gcgcggattc tgcatggcca
     1261 cgattgggtg ttcagtggcg aggtcctgaa ggtgtttgga aatcccgccg acggcgacgt
     1321 gatttcgctc agggatggcc gcgaccgtct tctggggagc gcaatttaca attccaagtc
     1381 gcagatcgtc gcgcgcagat tttcgcgccg gaaacaggag ctcgatcttg atttcttcca
     1441 acgccggatc gcgcaggcga tcgaataccg ggcgcgtcgc gggatcgagc cccgactctg
     1501 tcgcctcgtt tggagcgaga gcgacggact ccccggcgtg atcatcgatc gctacggcga
     1561 ttgtctcgtg ctccagacgc tcacgctggc aatggaccag cgcaaagagc ttatcgttag
     1621 ggcgctggaa acgcattttt gtagggcaag cgctccgctt gccgagggaa aagaggcagg
     1681 cggagcgccc gccctgcaat cgattatcga gcgcaacgat gccccggtgc gccgcgcgga
     1741 ggggatggaa ttacaaagcg gcctcctgtt cggcgtcgcg cctgacgaga tcgagatcga
     1801 ggtcgccgga ttgaaattgc agatcgatct gctccattcc cagaagaccg gtttttacct
     1861 cgatcaactc ccgaactacg cgctcgtcgc gcaccacgcc gcggggcgcc gggtgctcga
     1921 ttgtttcgcc agccagggtg gattcgcgct cgcctgcgcg cgcgccggtg ccagcgaagt
     1981 cgtcgctgtg gaagccggcg cggcgagcct gcgcgcggcg gaacaaaacg ccgtgcgcaa
     2041 tcaactagca gtgcgctggg tcgagcagga tgttttccaa ttcctccgcg gggccgagaa
     2101 agcggaagaa gaattcgacc tgatcattct cgacccgccc tcttttacga aaagcaaagg
     2161 cggcctgcac gatgccttgc gcggttacaa ggagctgcac gttcgcgcct tcaaattgct
     2221 ttcgcctcac ggaatgctgg cgaccttctc ctgctcgcat cacgtcggcg cggaaatctt
     2281 cgaaaaaatg atcgccgacg cgctcgtcga tgcgcgccgc tccgcgcggc ggctggcgcg
     2341 ttacgaacag gcgctcgatc atcccgtgct tccgaccctg ccggagacag aatattttcg
     2401 cgggacgctg ctcgagatga tgcccggtcg ttaggcggca gaatcaatcg ggccgtctcg
     2461 agaaaatccg aatccactcg tcgttggttc gccaaatgta gggctccgtg ggagtgacca
     2521 cgttgccgaa actagcgcgc ggcgagaagg aggcggaggt ggcgggtcga ggcgtggaga
     2581 agccggaaat caggtagtcg aattcctttc gttggagcaa agccacggca tcggaccatg
     2641 atgcgacttg gaccgaggcg ccaatgcgac tcagctcgct tggcaataaa ccaagttcgg
     2701 caatcgccag gacgcgatcc gttttccgaa cgcgcgcttg caaccaatcg accgcctgaa
     2761 tgcggctatc gcggtgaaaa attcggtagc gcaacggttc gaaggaagaa aaacccgcat
     2821 tgatgacgat cgccccgatg accagcgcga tgaccgggtt ccaaagcgcc gcttggccgc
     2881 tccggcccga ccaacccagg agttgatgga atgcaaacgc gccagcaata cagaatggcg
     2941 gaagcaaggg caggagattg cgaaagggtt gaaaaggttt cccgatgaaa agaccgatca
     3001 gaataactcc gaaaagaatc cagccgagcg ccacgggccg atcgatctcc gaacgaagca
     3061 tcaggaccaa cccaaggcag ccgacaatca ttaatggcca acctaactcg tagttagaca
     3121 cagcctgccc aaaatatccg ggcacggaag tcatcgtcgt atagccatgg gcgagcgcga
     3181 ggagagcccg gaagacagcc gcggaattag acaccgtcgc cggtgccatc agggctgccg
     3241 tgaaacagaa tccaccgctg gccagcccca gcaggcgcag gcggaaggtt ttgctgacgg
     3301 gcaaggtgat gagcgtgaac gccaccgcgg caaagaccac ggccaccgta tacttggaag
     3361 caaaggccaa tcccgccgcc gcgccggcat aggccgcccg ccggctcatc ctgccgtctc
     3421 ccatggcagg ctctttcacc agacaagcgc agaaaaacag cgaccaaaca gcaaaaaacg
     3481 ttgcgaaagt atcgacaatg atatttgacg cccgactggt tagggcggga caggtggcga
     3541 caagaatcag cgataaccgc tcgacccgat ctcccgccag aatgcctgca atcctacccg
     3601 ccaggaccac cgtcccgacg ctcagaaaaa tcgcaacgaa gcggccggcg acaatcaagg
     3661 ccgggggtga gatgagatca tagttgtccc ccttggaggt gtgaatgtct cgatcccttg
     3721 gcagatcctt acggaaacca tgcccatgca cgaggcgata gaccggcgaa taagcaatca
     3781 acgcggcggc cgtgaggtaa gccggtagcg aaggataata ataccatttc gtatccaggc
     3841 tgcggtggtt aagaagctcg attgactgat gcagcacata tccttcgtcg atataatcga
     3901 tatagggcag ccccgaacaa atcgtcggcc cccgcaatag aaccgccaag agaatggctc
     3961 cggcccagca gaatctgtgg agcagttggg ggaacgtcga cgaacaaaat tgcgctcgga
     4021 attgttctct gagacggttc accctttgat ataaggtctc ctcgggtcgt gaaatcaatt
     4081 ccgaatgcag agcctgattt tcgaaaaaga ctttcgactg caatctggga attgaccaag
     4141 gccggagctt gcaccgttgc gacaaggaaa tgcgaaaatc tgtcgtgaaa attctggcaa
     4201 tcgcggcgcg gccaattaat atgccgccgt agcgttcgcg cgaccggaca acaccagtcg
     4261 ccgggcgcac tcaaaacgcc atggccgaac gcatccgtcc gatcgaacaa atgctggaac
     4321 tcaacgaaga cgacagtctt ggcaccgagc acctcgactc ccaggtgc
//
LOCUS       DATMNS010000191         8667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_614237, whole genome shotgun sequence.
ACCESSION   DATMNS010000191 DATMNS010000000
VERSION     DATMNS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614237"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VNW28_06495"
     CDS             <1..837
                     /locus_tag="VNW28_06495"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXA09613.1"
                     /translation="VDAHRKIQASSKEKEEEYFKAVPVTITTEGDKVIVRAIHQRDSL
                     GKQLWRMFGHTRTDARYTLRVPSNFNLDLDTSGGDISTHDLTGKMKIDTSGGDLNFGQ
                     IHGEIHADTSGGDITAKDCDGTTALDTSGGRIEVTGGRGQLNVDTSGGNVTVLNRLGD
                     TKVESSGGKLRLGNISGKLNAETSGGSVSAILPSPVTGDVRLETSGGSITVLTPANAA
                     LTIDAETSAGGVRSDLPIARSSTESDSLKGTLNGGGTKLVLRSSAGSIEIVAAEKQTA
                     QQ"
     gene            957..3350
                     /locus_tag="VNW28_06500"
     CDS             957..3350
                     /locus_tag="VNW28_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09614.1"
                     /translation="MNDFRYAVRQLIKNPGFATVAILTLALGIGACAAIFSVVNGVLL
                     RPLDYPQPDRIVVMRETNLPQFPIFSVSPPNYLDWQKQTKSYSAIAAYSGDQINLTGE
                     GEPQRLVGVKATADYFKVYGVQPVLGRTFLPDEDAVGKNHVAVLSNPFWQRVFGGEKN
                     VIGRAVQLNGEAYTVIGVAPPDFGQQSKVDVWTPMAFSAEERDNKYRGAHYLGVVGRL
                     KPGVTVVQAKAELEVLAKQLARQYPDSNKGWGVFMMSMLDYSVRDVRVVLYTLVGAVG
                     CVLLIACANIANLLLARATARHRELSIRAALGANRFRLVRQLLTESVLLALCGGAAGL
                     LVANWGLAALLALAPEDLPRASGIQLNATVLIFSFLLSIVTGVVFGVAPAWLAAHTDM
                     NESLKQGSRGSTDARGRLRGALVVIEVAFALVLLGGAGLLARSFMRLTHVDPGFNPNN
                     ATVLRLALPEKKYEKNEQQVAFADALLEKLHVLPGVQEVGLTHTLPLLGDWVLTFEIA
                     GRPKLAPSDLPNTNYYAVTPGYFHAMGIPLIRGRLFTPQDNAKAPRVAIINETMAKQF
                     FPNEDPIGKQINVQNGPDTFRQIVAIVGDIKQYGVDKETTAQTYEPFAQHPFNTLNVV
                     LRSTGSPAALIGALRPAVYSIDKDQPVGTIRPLTEILSETIAKQRFAMTLVAVFSIVA
                     LIIASVGIYGVMSYNVVQRRSEIGIRMALGAQTRDVLSLILGSGGKLVGIGLLVGLVA
                     TFLATRAMGSILFQTNAYDPLTLGAITLLLAAVALVACLLPAHRATRVNPTEALRAE"
     gene            3409..5865
                     /locus_tag="VNW28_06505"
     CDS             3409..5865
                     /locus_tag="VNW28_06505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09615.1"
                     /translation="MNDLRFALRQLHRSPGFTVIAVLTLALGIGANTAIFSVLESTLL
                     RPLPFPHADRLVRLYELSGESGERSTRLNLSPVAWQQWREQSGQIFEGIGAATGASLT
                     LGARAGEPARNIPAALISANFLDVVGLQPALGRNFVAAEDRPGAERVAIVGYDFWRQQ
                     LGGRRDVLGTTVVLDGIPCAVVGVMPKTYRHPYRAEVWLPLRTDFSAPRSQHHYLYGV
                     ARIRPGLTLGQAQAATDRMCAAIKETTHDPASSARTELMLLRDSFILDLRPKLLIIAG
                     AALCALLVAAANFAGLLLARAVEHGGEMALRAALGASRGRLVRQGLVQSLLLTAAGTI
                     GGLLLAGWFTPLLVALSPEGSDTTGSAIREFDYAVRLDWPVFAFAVGAMLLVGVGVGL
                     FPAWRAARTDLREALSSVARGATLDGSTRRWLSGLIVVEIAVAAVLLVGSLSLTEYFR
                     AIVRQPWGFATDHRLVFSAMFAERLFPSPEARRRAIDRSLEELRALPGVRSASVTAPP
                     PMDASWDTIPCAPEGSHPPAPSGVFFAYLRAIGPGYFTTMGQPLLRGRDFNADDRAGS
                     PPVCIVNASFAQRFWPDEDPIGRRVKEGRFDGSHPWLTVIGITGDTKAVADPRDGEVV
                     GTLYFSLSQAIAAGFDEMSFVVETAGPALAASPGARGAMARADNRIAAYNVTSLENAA
                     TDSWVTERFLFVLVSLFGLLGLVLAAIGVYGLLALQVARRFREFGVRVALGATGATLV
                     RLVAGQGVRLLGLGFVTGALGAWAGVRVLQHEWPGVPTGNPLIWLGAAALLSLGVALA
                     SWFPARRAGRIDPVVALRSE"
     gene            5898..8357
                     /locus_tag="VNW28_06510"
     CDS             5898..8357
                     /locus_tag="VNW28_06510"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09616.1"
                     /translation="MNDFRFALRQLRKSPGFTVIAVLTLALGIGANTAIFSVVNAVLL
                     KPLPFPAPEQLIAIGSTNTHDGTPRDQLNSLSYPDFFDFRKQNRTLASSAVYRDQSVA
                     FTDANGAQSFLQVKCSAEFFDVLGIKPTIGRGFARTDEQAGGGPGGFKVILSHDFWQK
                     HFGGEQNVLGRTVELDRRPFTVIGVLPAHFQFPIQTESIEFYVTVAEDASSSDGSKPV
                     TEERGNHTLEAIGRLKPGISIAQAQADLSAIAANLEKQYPDTNSYFGALVKPLREELV
                     GDVRTALYVLFGAVACVLLIANANVANLLLARASVRGREIALRAALGASRGRIVRQLL
                     TESVVLSALGGGLGLLVAQWGTAGLIHAVPQNIPRIGDIQLDAAVLAFTLFVSLATGV
                     IFGLVPAWQASHIDLNSALKSGLRTGGDSAHKGRVRNALVMAEVALALVLLICAGLLI
                     QSFARLGQVPTGLRPDRLLTARFDLPGSAYPKNENIIAFFQRLMPKIRALPGVEAAST
                     IMPLPLSSSNMVTDFDLADHPLPEGQRPAAPARVAGTDYFRTMGIPVRQGRVFDAHDG
                     FDTTPVVIVNELFASKYFPGQNAIGKQITPGFSTDDEKPKMREIIGVVGNVKHRSLRN
                     EDTPEMYLPQTQIPFDRMTLVVRTGVANPASLTGAIRAQLAAVDPNIPLTNVRVFEEY
                     ISRSLARPRFNALLLSIFAGTALLLTAIGIYGVMAYSVAQRTNEIGIRIALGAAQSSI
                     FRLIVGQAMVLVAVSVGIGLIGAVATTRLLNTLLFGVGAWDPLTFGAIVFLMAAVAFL
                     AAWLPARRAARVDPIIALRTE"
BASE COUNT         1450 a         2882 c         2675 g         1660 t
ORIGIN      
        1 gtcgatgccc accggaaaat ccaggcctcc tccaaggaga aggaggaaga atacttcaag
       61 gcggttccgg tcacgatcac caccgaaggc gacaaagtca tcgtgcgcgc catccaccag
      121 cgcgattcgt tgggcaagca gctgtggaga atgttcggcc acacccggac cgacgcgcgc
      181 tacaccctgc gcgtcccgtc caatttcaac ctcgatctcg atacctcggg tggcgacatt
      241 tccacgcacg acctcaccgg aaagatgaag atcgacacca gcgggggcga cctgaatttc
      301 ggtcaaatcc acggtgagat tcacgccgac acgagcggcg gcgatatcac ggcgaaggat
      361 tgcgatggca cgaccgccct cgacaccagc ggaggacgga tcgaagtgac gggtggccgc
      421 gggcaattga atgtcgatac ctcgggtggc aacgtcacgg tgctgaaccg tctcggcgac
      481 acgaaagtgg agagcagcgg cggcaaactg cggctcggca acatcagcgg taaactgaac
      541 gccgaaacat ccggcggatc ggtttccgcc attcttccct cgcctgtgac cggcgatgtg
      601 cgcctcgaaa cctccggagg cagcatcacg gtgctgacgc cagcgaacgc cgctctcacg
      661 atcgacgccg aaaccagcgc aggcggggtg cggagcgatt tgcccatcgc gcggagcagc
      721 accgagagcg attcgctcaa gggcacgttg aacggcggcg gcacgaaact tgtcctgcgc
      781 agcagcgccg gttcgatcga gatcgtcgcc gccgaaaagc aaactgcgca acagtaatga
      841 gcaccgttac acagccatcc tggctgtctc gtagtgcggg cgtctcgccc gccggatgca
      901 atgaacctgc gggcaagatg cccgcacgac cgatcaggcg ggacgcctga attacgatga
      961 acgattttcg ctatgccgtt cgccagttaa tcaagaaccc gggtttcgcc acggttgcaa
     1021 ttcttaccct cgccctcggg atcggcgcct gcgccgcgat tttctccgtc gttaacggcg
     1081 tcctcctgcg gccgctcgat tatccgcaac ccgatcgcat cgtcgttatg cgcgagacga
     1141 atctgccgca gttcccaatc ttctccgttt cgccgccgaa ctatctggac tggcagaagc
     1201 agacgaaatc gtattccgcc atcgcggcct acagcggcga tcagatcaac ctcaccggcg
     1261 aaggcgaacc gcagcggctc gtcggggtga aggcgaccgc agattacttc aaagtctacg
     1321 gcgtgcagcc ggtgctcgga cggacgttcc tgccggacga agacgcggtc gggaagaatc
     1381 acgtcgcggt tctgagcaat ccgttttggc aacgcgtctt cggcggcgag aagaacgtca
     1441 tcgggcgcgc cgtgcagttg aacggcgagg cctacacggt gatcggtgtg gcgccgccgg
     1501 attttgggca gcagagcaag gtcgatgtgt ggacgccgat ggccttcagc gcggaggagc
     1561 gcgacaacaa atatcgcggc gcgcattatc tcggcgtcgt cggtcgcctg aaaccgggcg
     1621 taaccgtggt gcaggcgaag gcggagctgg aggtgcttgc gaagcagctc gcgcggcagt
     1681 atcccgactc gaacaaaggc tggggcgtct tcatgatgtc gatgctcgac tacagcgtgc
     1741 gcgatgtgcg cgtcgtcctc tacacgctgg tcggcgcggt cgggtgcgtg ctcctgatcg
     1801 cctgcgcgaa tatcgcgaat cttttgctcg cacgcgcgac ggctcggcat cgcgagttgt
     1861 cgatccgcgc cgcgctcggg gcgaaccggt ttcgactcgt taggcaattg ctcaccgaaa
     1921 gtgtgctcct cgcgctttgt ggaggggcgg ctggtttgct tgtcgcgaac tggggactcg
     1981 ccgccttgct cgccctcgcg ccggaagatc tgccgcgggc cagcggcatt cagctcaacg
     2041 ccaccgtcct gattttttcc ttcctcctca gcatcgtcac cggtgtggtc ttcggcgttg
     2101 cgccggcctg gctcgccgcg cataccgaca tgaatgaatc gctcaagcaa ggctcgcgcg
     2161 gctcgaccga tgcgcgcggc cggctgcgcg gtgcgctcgt tgtgatcgaa gtagccttcg
     2221 cgttggtctt gttgggcggg gcgggattgc tggcgcgcag tttcatgcgg ctcacgcacg
     2281 tcgatccggg attcaatccg aacaacgcga ccgtcctgcg cctcgcgctg ccggagaaaa
     2341 aatacgagaa gaacgaacag caggtggcct tcgccgacgc gctcctcgag aagctgcacg
     2401 tgctgcccgg cgtgcaggag gtcgggctca cccacacctt gccattgtta ggcgattggg
     2461 tcctgacgtt tgaaatcgcc ggccggccga agctcgcgcc gagcgatttg cccaacacca
     2521 actactacgc ggtcacgccc ggttacttcc acgccatggg cattccgctc attcgcggcc
     2581 ggcttttcac tccgcaggac aacgccaagg cgccgcgcgt cgcgatcatc aacgagacga
     2641 tggcgaaaca attcttccct aacgaggacc cgatcggaaa acagatcaac gtgcagaacg
     2701 gtccggacac ctttcgccag atcgtcgcca tcgtcggcga catcaaacaa tacggcgtcg
     2761 acaaggagac gacagcgcag acttacgagc cgttcgcgca acacccgttc aacaccctca
     2821 acgttgtcct gcgcagcacg ggctcgcccg cggcgctgat cggggcgctc cgacccgcgg
     2881 tttattccat cgacaaagat cagccggtcg gcacgatccg gccgctcacg gaaattctct
     2941 cggagacgat cgcgaaacag cgcttcgcca tgacgctcgt cgccgtcttc tcgatcgtcg
     3001 cgctgatcat cgcgtcggtc gggatttacg gcgtcatgtc ttacaacgtc gtgcaacgcc
     3061 ggagcgagat cggcatccgc atggcgctgg gcgcgcagac gcgcgatgtg ctcagtctca
     3121 ttctcggcag cggtggcaag ctggtcggga tcggattgct cgtcggcctg gtcgcgacct
     3181 ttctcgcgac ccgcgcgatg ggctcgattc ttttccagac gaacgcctac gatccgctca
     3241 ccctcggggc gatcacgctc ttgctcgcgg cggtcgcgct cgtcgcctgt ctgctcccgg
     3301 cgcaccgcgc gaccagggta aatccgacgg aggctctgcg ggccgagtga agcctggcga
     3361 atcattcgag cgtcgtcatt ttttccaccg tcacaagccg cgaactcaat gaacgacctc
     3421 cgcttcgcgc tccgtcaact ccacagatcg cccggcttta cggtcatcgc cgtcctcacg
     3481 cttgcgctcg ggatcggcgc gaacaccgcc atcttttcgg ttctggaaag caccctgctc
     3541 cggccgctgc cctttcccca cgccgaccga ctcgttaggc tgtacgaact ttcgggcgag
     3601 agcggcgagc ggagcacgcg cttgaatctt tcgcccgtgg cctggcaaca atggcgcgag
     3661 cagagcggcc agattttcga gggcatcggc gctgccaccg gcgcgagcct caccctcggt
     3721 gcccgggcgg gcgaaccggc acgcaacatt cccgcggcgc tgatcagcgc aaactttctc
     3781 gacgtggtgg gattgcaacc ggcgctcggc cgtaacttcg tcgcggcgga agaccggccg
     3841 ggtgcggagc gggtcgcgat cgtcggctat gatttctggc ggcagcagct cgggggacgg
     3901 cgcgacgttc tcggcacgac cgtcgtcctc gatggaatcc cctgcgccgt tgtcggcgtg
     3961 atgccgaaga cctaccggca cccatatcgt gcggaggtct ggttgccgct tcggaccgat
     4021 ttttccgcgc ccagatcgca gcaccattat ctctatgggg tggcgcgaat ccggcccggg
     4081 ctcaccctcg ggcaggcaca ggccgcgacg gatcgcatgt gcgctgcgat caaagaaacg
     4141 acgcacgatc ccgcaagctc tgcgcgcacc gaactgatgc tcttgcgcga cagcttcatc
     4201 ctcgatctcc ggccgaaact cctcatcatc gcgggtgccg cgctctgcgc gctgctcgtc
     4261 gcggcggcga attttgccgg cctgctcctc gcgcgcgcgg tcgagcacgg cggcgagatg
     4321 gctttacgcg cggccctcgg cgccagtcgc gggcgactcg ttaggcaagg tctcgtccag
     4381 tcactcctcc tcaccgccgc cggcacgatt ggggggctgc tcctggccgg ctggttcacg
     4441 ccactcctcg tcgcgctcag tcccgagggc tccgacacca cgggcagcgc gattcgcgaa
     4501 ttcgattacg cggtccggct cgactggccc gtcttcgcct tcgcggtcgg ggcgatgctg
     4561 ctggttgggg tcggcgtcgg cctcttcccc gcgtggcgcg cggcccggac cgatttgcgc
     4621 gaagcgctga gcagcgtggc ccgcggcgcg accctcgacg gcagcacccg gcgctggctg
     4681 agcggactta ttgtcgtgga aatcgcggtc gccgcggtcc tgctcgtcgg cagcctgtcg
     4741 ctcacggaat attttcgcgc gatcgttagg caaccttggg gatttgcgac tgatcatcgg
     4801 ctcgttttta gcgcgatgtt tgccgaacga ctcttcccat cgccggaggc gcggcggcgc
     4861 gcgatcgatc gctcgctgga ggaactgcgc gcgctccccg gggtccgctc cgcgtccgtc
     4921 accgcgccgc cgccgatgga cgcgtcctgg gacacgattc cctgcgcgcc ggaaggaagt
     4981 catccgccgg ccccaagtgg agttttcttt gcctacctgc gcgcgatcgg cccgggctac
     5041 ttcacgacga tgggccaacc cctgctccgc ggccgcgatt tcaatgcgga cgatcgcgcg
     5101 ggttcgccgc cggtctgcat cgtcaacgcg tcgttcgcgc agcggttttg gcctgacgaa
     5161 gatccgattg ggagacgcgt gaaggagggc cgcttcgatg gctcacaccc ctggctcacg
     5221 gtcattggca tcaccggtga caccaaggcg gtggccgatc cgcgcgacgg ggaagtggtc
     5281 ggcactcttt atttttcgct ctcgcaggcg atcgcggcgg gattcgacga gatgagtttc
     5341 gtggtggaga ccgcgggccc ggcgctcgcc gcttcccccg gggcgcgagg cgcaatggcc
     5401 cgcgcggaca accgcatcgc cgcctacaac gtcacgtcgc tggaaaatgc tgcgacggat
     5461 tcctgggtga cggaacgttt tctttttgtc ctggtctcgc tcttcggatt gctcggactc
     5521 gtgctcgcgg ccatcggagt ttacggattg ctcgccttac aggtcgcgcg ccgcttccgc
     5581 gagtttggcg tgcgcgtcgc tctcggcgcg accggcgcga cgctcgttag gctggtcgcc
     5641 ggccagggcg tccgcttgct cggccttggt tttgtaacgg gcgcgcttgg tgcctgggcc
     5701 ggggtgcgcg ttctgcagca tgaatggccg ggcgtgccga cgggcaatcc gttgatctgg
     5761 ctcggtgccg ccgccctcct ctccctcggc gtcgcgctgg cctcgtggtt tcccgcccgc
     5821 cgcgccgggc gcatcgatcc cgtggtggcc ctccgttccg aatgaatttc taccttgcct
     5881 aacgaatcga actttacatg aacgacttcc gcttcgctct ccggcagctc cgcaaatcgc
     5941 ccggctttac cgtcatcgcc gtcctcacgc tcgccctcgg aatcggggcc aacaccgcca
     6001 tcttcagcgt ggtcaacgcc gttctcctca agccgctgcc ctttcccgcg cccgaacaat
     6061 tgatcgccat cggcagcacc aacacacatg acggcacgcc gcgcgatcag ctcaactcgc
     6121 tctcctatcc cgatttcttc gatttccgga aacagaatcg caccctggcg agcagcgcgg
     6181 tttatcgcga ccagtcggtc gcgttcaccg atgcgaacgg cgcgcaaagc tttctccaag
     6241 tgaagtgcag cgccgaattc ttcgacgtcc tcgggatcaa gccaacgatc ggacgcggat
     6301 ttgcccgcac cgacgagcag gccggaggtg gcccgggcgg gttcaaggtc attctgagcc
     6361 atgacttttg gcagaagcat ttcggcggcg agcaaaatgt gctcgggcgc acggtcgaac
     6421 tggatcgacg gcccttcacg gtcattggcg tgctgccggc gcactttcaa ttcccgatcc
     6481 agacggagtc gatcgagttc tacgtgacgg tcgctgaaga cgcctcatcc tcggacggat
     6541 caaagccggt gacggaagag cgaggcaacc acaccctcga agcgatcggg cgactcaagc
     6601 ctggcatttc gatcgcgcag gcgcaggccg atctctcggc catcgccgcc aatctcgaaa
     6661 agcaataccc tgataccaac tcctatttcg gcgcgctcgt gaagccgttg cgggaggagc
     6721 tggtcggtga tgtccggacg gcgctctatg ttttgtttgg cgccgtcgcc tgcgtcctcc
     6781 tcatcgcgaa cgcgaacgtc gccaatcttc tgctcgcccg cgcctcggtc cgcggcaggg
     6841 agatcgcctt gcgggcggcg ttaggcgcga gccgcgggcg catcgtgcgg caactgctca
     6901 ccgaaagtgt cgtgctctcc gcgctcggcg gcggtctggg cctgctcgtc gcgcagtggg
     6961 gcaccgccgg gttgatccac gccgtcccgc agaacattcc gcgcatcggc gacatccagc
     7021 tcgatgccgc ggtccttgcc ttcacccttt tcgtctcgct cgccaccggc gtcattttcg
     7081 ggctcgtccc ggcctggcag gcctcgcaca tcgacctgaa cagcgcgctc aagagcggcc
     7141 tgcgcaccgg cggcgacagc gcgcacaagg gccgggtgcg caacgcgctc gtcatggcgg
     7201 aagtggccct cgcgctcgtc ctcctcatct gtgcgggatt gctcattcaa agtttcgcgc
     7261 ggctcggcca ggtgccaacc ggactgcgcc cagaccgcct tttgacggcg cgcttcgact
     7321 tgccgggatc ggcctaccca aagaacgaaa acatcatcgc gttcttccag cggttgatgc
     7381 cgaaaattcg cgcccttccg ggcgtggaag cggcgagcac gatcatgccg ctgcccttga
     7441 gctcgagcaa catggtgacg gatttcgacc tcgctgatca tccgctgccg gaaggccaac
     7501 ggcccgccgc accggcgcgg gtcgcgggga cggattactt caggacaatg ggcattccgg
     7561 tgcggcaagg ccgggttttc gacgcgcacg acgggttcga taccacgccg gtcgtgatcg
     7621 tgaacgaact cttcgcgagc aagtatttcc ccgggcaaaa cgcgatcggc aaacagatca
     7681 cgcctggttt cagtaccgat gatgagaaac caaaaatgcg cgaaatcatc ggcgtggtcg
     7741 gcaatgtgaa acaccgctcg ctgcgcaatg aagacacccc cgagatgtat ctgccgcaga
     7801 cgcagattcc gttcgaccgc atgaccctcg tcgtgcgcac cggggtcgcg aatcccgcca
     7861 gcctgaccgg ggcgattcgc gcgcaacttg ccgcggtcga tccgaatatt cccctaacga
     7921 atgtaagggt ctttgaggag tacatctcgc gctcgctggc ccggccgcgt ttcaatgctc
     7981 ttctcctctc catcttcgcc gggaccgcgc tcctcctcac cgccatcggc atctacggtg
     8041 tgatggccta ttccgtcgcg cagcggacga acgagatcgg catccgcatc gcgctcggcg
     8101 cggcgcaatc ttccatcttc cggcttatcg tcggccaggc gatggtcctg gtcgcggtca
     8161 gcgtcgggat cgggctgatc ggcgcggtcg ccaccacccg cttgctcaac accctgctct
     8221 tcggagtggg tgcgtgggac ccgctgacct tcggcgcgat cgtgttcctg atggcggcag
     8281 tggcttttct cgccgcctgg ctgccggcgc gtcgcgccgc gcgagtcgat cccattattg
     8341 ccctgcgcac ggaatgaaac tcccgcctaa cgaggacgtc ttgtgttgac cgatttcaaa
     8401 tacgccctgc ggatgcgagg cgcacgacgt tttcacctac tcgagcgacg tcttattgtt
     8461 aggcgtgacc gccttgctcg ccagctatat cccggcgcgt cgcgccatga aagtcggccc
     8521 gatggtcgcc ctccgctatg agtgattttc gtttcacctt tcgtcagctg cgcaaatcgc
     8581 ccggctttac cttcgtcgcc gttctcactc tctcgttagg catcggcgca aacaccgcta
     8641 tctttagcgt gattaatgcg gtgttgc
//
LOCUS       DATMNS010000192        12196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_615893, whole genome shotgun sequence.
ACCESSION   DATMNS010000192 DATMNS010000000
VERSION     DATMNS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 12196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12196
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615893"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VNW28_06515"
     CDS             complement(<1..386)
                     /locus_tag="VNW28_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HXA09617.1"
                     /translation="MDETLNDSVDALLRTYSADGGINYLDAAATLPSRPAIDAACVEL
                     LSLLFPGFHGEPVIQPGDLPELTRSRIRSLRTRLRPEMCKSLAREGSNGAVEPEADRI
                     LTDFFSKLASVREMLWNDIDAAYEGDP"
     gene            581..2848
                     /gene="glgB"
                     /locus_tag="VNW28_06520"
     CDS             581..2848
                     /gene="glgB"
                     /locus_tag="VNW28_06520"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXA09618.1"
                     /translation="MATDAVSLLFHFMSSFAIHPLDPNEINALLRASHSDPFRVLGPH
                     RAGDDLVIRVFRPEARGIEIVAADDQAQVFPAERLHIEGIYQATIPGQSAGFSYFVKV
                     LAFDGSELLLRDPYSYGPIMGDVDLHLFAEGNHKQLYEKFGAHIRVVGGERGVYFAVW
                     APNAARVSVVGDFNGWDGRVHPMRKLLAGGVWEIFIPGVNEGAHYKFEIKTQTGALLL
                     KSDPFGFFSQHGAQTSSMVYNLERYQWSDDQWMAERPQKQWHRSAVSIYEVHLGSWRR
                     VAEEGNRFVSYLEFAQTLLPYVLEMGFTHIELLPIAEHPFEGSWGYQVTNYYAPTSRF
                     GTPDELRHFIDACHRAGIGVIMDWVPAHFPKDAHALAQFDGTALYEHADPRQGEHQDW
                     GTLIFNFGRNEVRNFLTANALFWLDKYHIDGLRVDAVASMLYLDYSRQPGQWIPNAFG
                     GRENLDAVFFLKQANELCYEGFPGVMTIAEESTAWPGVSRPTYLGGLGFGFKWNMGWM
                     HDFLHYMSLDPIYRRFHQGNVTFSIMYAFQEHFILVLSHDEVVHGKGSLISKMPGDEW
                     QKFANLRMFYAWMFAHPGKKLLFMGGEFGQVREWNHDRGLDWELTELPRHDGLRRLVQ
                     HLNHVYRNEPALWDRDDSHESFEWIDFHDADHCVVAFLRRSQEGELLVFAVNATPVVR
                     YNYRMGVPGDGFYREIINTDAETYGGSNVGNLGGLDAEAVEWQGRTHSIVVNLPPLAV
                     VAFRREAAGEPVTAS"
     gene            3059..3760
                     /locus_tag="VNW28_06525"
     CDS             3059..3760
                     /locus_tag="VNW28_06525"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXA09619.1"
                     /translation="MKKTDSAFTLIELLVVITIIAILASIALPVFNGVTERANQTKDL
                     SNAKQVALALKVFATDNNGFFPSKSPSGDYNSATPLAAGAVSNDAFWWLFPNYLQSED
                     VFAVASSQWSPASPDNKTDPATATTRVDTLKAGENTYSYVLGLTDTSNAAFPLLADGF
                     SQATPIAYETSKSLKGGVWAGKKAIVIFCDASGQIVKVDSNTPHVVKRPTGENLFASA
                     ADWLDATANPVINPQ"
     gene            complement(3775..5133)
                     /gene="xseA"
                     /locus_tag="VNW28_06530"
     CDS             complement(3775..5133)
                     /gene="xseA"
                     /locus_tag="VNW28_06530"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HXA09620.1"
                     /translation="MNSDTDLFPNIARVLTVAELTRAIRDLLASRFGAVWVQGEISNY
                     KRHPSGHQYFTLKDQRAALNCVIFRDTLPPSSTPLADGMQVQVYGNITVFEMRGQYQL
                     SVQILQSRGLGLLQAKFEALKRKLEAEGLFETARKRPLPKFPRRIGIITSPSGAAIQD
                     MLNILRRRAPGVEVLISPVRVQGTGAALEIASAIRELNAPNESWKALDVLVVTRGGGS
                     IEDLWEFNEEIVARAIFHSAIPVVSAVGHEIDFTIADFAADLRAPTPSAAAELIVPDA
                     RDLARRMQELKNSLEKCWRNFLAQARGRLSGVSDKVLFRELSQRLRNAQQQLDLSRES
                     FRRAVERRQITERTRLTNAQLALKAHHPARELMARKQELREIGRRLGECSKQRLSAAR
                     QRWERLSGMLRILGPEATLERGYSITRDAQGNVIRRVAEAKTRTRIKTRVRDGEFEST
                     VS"
     gene            complement(5314..6327)
                     /gene="ruvB"
                     /locus_tag="VNW28_06535"
     CDS             complement(5314..6327)
                     /gene="ruvB"
                     /locus_tag="VNW28_06535"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HXA09621.1"
                     /translation="MPAVVPSAPDPAFEISLRPPMFSEFTGQVKVCERLAVLVEAAKK
                     RGDVLEHILLSGPPGLGKTTLAHIIANAMGVNIKNTSGPVIEKAGELAGLLTSLEKGD
                     VLFIDEIHRLQPTIEEYLYPAMEDYKLDIIIDQGPQARSLQLRLPKFTLIGATTRSGM
                     LSAPLRSRFGMTARLDYYSAEEMQKIILRSAGLLNVEIDAAGAAEIATRTRGTPRTAN
                     NLLRWVRDYVQVKAEGRITADLADRALTMLEIDRDGFDQWDKRIIETLIYKFNGGPVG
                     LSSLAVAVGEEAGTLEEVNEPYLIMEGYIKRTAQGRVALPNAYRKLGLKAPAGPQSEL
                     FGS"
     gene            complement(6409..6816)
                     /locus_tag="VNW28_06540"
     CDS             complement(6409..6816)
                     /locus_tag="VNW28_06540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXA09622.1"
                     /translation="MKKTKKKIEFSSHTGNLALMRNCVRRFLEAFPFSERQRTLMVLG
                     VDEACTNIIRHAYQLRDDRLISLSLEGRNDCVCLRLRDYGKQAPPNAMQSRPPDLIKP
                     GGLGLYLIRNAFDQVDYVLRSRGTELVLTKNLE"
     gene            7084..7803
                     /locus_tag="VNW28_06545"
     CDS             7084..7803
                     /locus_tag="VNW28_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09623.1"
                     /translation="MSKWFIALLLLATPGFAQERPTAYQALRTIGIQLKRDLVNHVIS
                     VTGTNGDPQPPTWKILLDDPQARGGVREVEVGNGQIISERTPLRSSVEGSLGAVIDTS
                     KLNLDSSGAYTVARQTAAKSHVTFATADYALRADERGNPMWMVTLQSQGGESAGKIFI
                     GANHGTITRTEGFFSGGDRTAVVDEQSDEPNTMDADDADDGDHNIVERRVQQAFREAR
                     DDVKRTFHKVRRSFVDFFQDK"
     gene            7963..8898
                     /locus_tag="VNW28_06550"
     CDS             7963..8898
                     /locus_tag="VNW28_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXA09624.1"
                     /translation="MKVPLPEQTAWKNVSHGWQQLYGSFEDLGISIEWHDFQTSQPLD
                     WAESFRPGSIEFCLNLEGRGAIGCRSSASHDYGPSSSGYYAIGDEPLHATRQAHDRHR
                     FVTLEYSRPYLQTQVAESESELAPAMRAVIFPKKEESIVSPPQPMSAEQRAIVASLSE
                     PPVPKAARIIWYRSKALELMSHFLFEAKDPEFFCMRQKRVARDRVLRTKELLVRDLAN
                     PPTLEMLGQEVGCSPFYLSRIFSREVGLTIPQFLRNARMERAAELLRSGRYNVTEAAT
                     EVGYSSLSHFSKAFCETIGCCPVLYPAAKHIVLDR"
     gene            8905..9720
                     /locus_tag="VNW28_06555"
     CDS             8905..9720
                     /locus_tag="VNW28_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09625.1"
                     /translation="MSAAAASAGARRSGLFFLPTVTLWQREIVRFYRQRNRIIGALLT
                     PFVFWFLIGSGIGPSFRAGSSPNGVGYLEYFYPGTLILIMLFTAIFSTISIIEDRREG
                     FLQSVLVAPISPGSIVCGKILGGTTLALMQGFIFLLLAPFVGIHITPERLLVCLGTLF
                     VVGFGLTGLGFLVAWPMESTQGFHAIMNVFLIPLWLMSGALFPATNPHSWIFWAIHLN
                     PVTYGVAAVRRALYAADPGVLYLPSFTLSIGLSVAFGLIMYVIAYFLVQRKSS"
     gene            9717..11249
                     /gene="purH"
                     /locus_tag="VNW28_06560"
     CDS             9717..11249
                     /gene="purH"
                     /locus_tag="VNW28_06560"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HXA09626.1"
                     /translation="MKIKRALISVSDKSGVADFARDLEKQGVEIISTGGTASLLRQEG
                     IKTRDISSFTGFPEILDGRLKTLHPKVHGALLYLRGNNEHEAAAREHGIETIDLLAVN
                     LYPFEATIAKPGVNLAAAIEQIDIGGPAMLRSASKNYQSVTVVVDPADYQSVLDAMHD
                     REGETTLKLRERLAIKAFLKTSDYDRAIASYLNEKQETETSFSLSLPLVSRLRYGENP
                     HQTASLYGTFADHFEKLHGKDLSFNNLLDITAAADLIDEFSEPTVAILKHTNPCGVGT
                     DPDLREAWLKAFATDKQAPFGGIIICNRPLDLPVAKAISEIFSEVIIAPEFATDARAL
                     LQKKKNLRLMRTLQPEVPNEKPAMDVRSVRGGLLVQQKDAAELEGLETKVVTKRPPTK
                     QEMAAMLFGWRVVKHVKSNAIVYASADRTLGIGAGQMSRVDASRIAVWKAAESGLSLK
                     GSAVASDAFFPFADGLVAAAEAGATAAIQPGGSVRDEEAIAAADERGMAMVFTGVRHF
                     RH"
     gene            complement(11921..>12196)
                     /locus_tag="VNW28_06565"
     CDS             complement(11921..>12196)
                     /locus_tag="VNW28_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09627.1"
                     /translation="GRVAIAVAAAFIANHAHYSIITFVVGGILGAFLLLGLFDWALII
                     FSAIEGARLLASSIHLPSTGTTVLVVALTIFGIVVQSAMFRRHRRIA"
BASE COUNT         2319 a         3700 c         3677 g         2500 t
ORIGIN      
        1 ggatcgcctt cgtaggccgc gtcgatgtcg ttccagagca tctcccgcac actggcaagc
       61 ttggaaaaga agtccgtcag gatgcgatcg gcttcaggtt cgaccgcgcc gttgctgcct
      121 tcgcgagcaa ggctcttgca catttccggg cggagccggg tgcgaagcga gcgaatccgg
      181 ctgcgggtga gttcaggcag atcgcccggc tgaattaccg gctcgccatg aaaaccaggg
      241 aaaagaaggc tgagcaattc gacgcaggcg gcgtcgatcg ccggccgcga ggggagggtg
      301 gcggcggcgt cgaggtaatt gatgccgccg tcggcgctgt aggtgcgcaa caacgcgtcg
      361 actgaatcgt tcagagtctc gtccatctgg gcgaacatcg gtgaggtagg agggattgaa
      421 aatcgaaaag gtttgctcga tgtcgtcatc ctgagcggag cgcagcgcag tcgaaggatc
      481 ccgtcgcgta acgttccccc ctggtgcaag ctttaagatt actccgcggg atcgctcgac
      541 ttcgcttggg atgacggcgg ccttcgccgt cacaattgca atggcaaccg acgccgttag
      601 tttgcttttt cacttcatga gctcctttgc catccacccg ctcgacccta acgagatcaa
      661 cgcgctcctg cgtgcttccc attccgatcc tttccgggtg ctcggtccgc atcgcgcggg
      721 cgacgacctg gtgatccgcg tttttcgacc ggaggcgcgg gggattgaaa tcgtggcggc
      781 ggacgatcaa gcacaggtct ttccggccga acgcctgcac atcgagggaa tttaccaggc
      841 gacgattccc ggccagtcgg ccggtttttc ctacttcgtg aaggtgctcg cgttcgatgg
      901 ctccgaactg ttgctccgcg atccctacag ctacgggccg atcatgggcg acgtggatct
      961 gcatctcttt gcggaaggaa atcacaagca actctacgaa aaattcggcg cgcatatccg
     1021 cgtcgtcggc ggcgagcgtg gcgtctactt cgcggtctgg gcgccgaatg ccgcacgggt
     1081 cagcgtggtc ggcgatttca atggctggga tggccgcgtt catccgatgc gcaagctgct
     1141 cgcgggcggc gtctgggaaa ttttcatacc cggagtcaac gaaggcgcgc attacaaatt
     1201 cgagatcaag acgcagaccg gagcgcttct cttgaagagc gatccgtttg gatttttcag
     1261 ccaacacggt gcacagacct cgtcgatggt ctacaacctc gagcgctacc aatggagcga
     1321 cgaccaatgg atggcggagc gcccgcagaa acaatggcac cggagcgcgg tcagcattta
     1381 cgaagtgcat ctcggctcgt ggcggcgggt ggcggaggag gggaatcggt tcgtcagcta
     1441 tctcgagttt gcgcagaccc tgctgcccta tgtactcgaa atgggcttca cccacatcga
     1501 gctgctgccc atcgccgagc atcccttcga aggctcgtgg ggttatcagg tgaccaacta
     1561 ctacgcgccg accagccgtt tcggcactcc ggacgagctg cgccatttca tcgatgcctg
     1621 tcatcgcgcc ggcatcggcg taatcatgga ttgggttccc gcgcattttc ccaaggatgc
     1681 gcacgcgctc gcgcaatttg acggcacggc tctctatgag cacgccgatc cgcggcaggg
     1741 ggagcaccag gattggggca cgttgatttt taactttggc cgcaacgagg tgcggaattt
     1801 cctgaccgcg aatgcgctct tctggctcga caagtatcac atcgacggtc tgcgggtcga
     1861 tgcggtcgcc tcgatgcttt atctcgatta ctcgcgccag ccgggacaat ggatcccgaa
     1921 tgcgttcggc ggccgcgaga atctcgacgc cgtctttttc ctcaaacagg caaatgaact
     1981 ctgctacgaa ggtttccccg gcgtgatgac gatcgccgag gaatcaaccg cctggccggg
     2041 cgtgtcgcga ccgacgtatc tcggcggtct cgggttcggg ttcaaatgga acatgggctg
     2101 gatgcacgac ttcctgcact acatgtcgct cgacccgatc taccgccgct tccatcaggg
     2161 gaacgtcacc ttttcgatca tgtacgcgtt ccaggaacac ttcatactcg tgctcagcca
     2221 tgatgaggta gtgcatggca agggttcgct catctccaaa atgccgggcg acgaatggca
     2281 gaagtttgcc aatctgcgga tgttttacgc gtggatgttc gcccacccgg gcaaaaaact
     2341 gctcttcatg gggggcgagt ttgggcaggt gcgggagtgg aatcatgatc gcggtttgga
     2401 ttgggaacta acagaattgc cgcgccacga cgggctgcgt cgcctggtgc aacacctcaa
     2461 ccacgtctat cgcaacgagc ccgcgctctg ggatcgcgac gactcgcacg agagcttcga
     2521 atggatcgat ttccacgatg ctgaccactg cgtggtcgcg ttcctgcgcc gctcgcagga
     2581 gggcgagctg ctcgtcttcg cggtcaatgc gaccccggtc gtgcgctaca attaccggat
     2641 gggcgtgccc ggcgacggct tctatcgcga gatcatcaat accgacgccg agacttacgg
     2701 cgggagcaac gtcggcaacc tcggggggtt ggatgcggaa gcggtcgagt ggcaggggcg
     2761 cacgcattcc atcgtggtga atctgcctcc gctcgcggtc gtggccttca ggcgcgaagc
     2821 agcgggcgag ccggtgacgg cctcctgacc ggctggctgc aagggcgaaa aagcgcccct
     2881 gagcggatca ccgagccggt caagggtaat gtccgttcat tgtcagccgc gggcggctac
     2941 aacggcgaca ggccgcaacg ggcaatcgac gaacaccaac tgactaaaaa tactctatct
     3001 ttgttgcttg acgctccgtg ttctcgcacc cagtctggcc cttgttaatc ttcttatcat
     3061 gaaaaaaaca gattccgcct tcaccctcat cgaacttctt gtcgtcatca cgatcatcgc
     3121 gattctggcc agcattgcgc tgccggtatt taacggtgtg accgagcgtg ccaaccaaac
     3181 caaagacctt tccaacgcca agcaggttgc gctcgcgttg aaagtattcg cgactgataa
     3241 caacggattc ttcccgtcga agagcccgtc gggggattat aattcggcga cacctctcgc
     3301 ggccggcgcg gtctcgaacg atgccttctg gtggcttttc ccgaattacc tgcagtccga
     3361 agacgtcttt gccgtcgcct cgtcgcaatg gtcgccggct agtcctgata acaagacaga
     3421 tcctgcgact gcaacgactc gggtcgatac gctcaaggcg ggtgaaaata cgtattcgta
     3481 tgttctcgga ttaacggata cgtccaatgc ggcgtttcct ttgctggcgg atggtttcag
     3541 ccaggccacg ccgattgctt acgaaaccag caaatctctg aagggcggtg tctgggcagg
     3601 caagaaggcg atcgtcattt tctgtgacgc gagcgggcag atcgtgaagg tcgattcaaa
     3661 caccccccat gttgttaagc gcccaactgg cgaaaatctt ttcgcttcgg ctgcggactg
     3721 gcttgatgca acggccaatc ccgtcatcaa cccgcaataa gcggcggcgg ctggttagct
     3781 caccgtcgat tcgaattcac catcgcggac gcgggtcttg atccgcgtcc gcgttttggc
     3841 ttccgcgacc cgccggatga cgttgccttg cgcatcgcgc gtgatgctgt agccccgctc
     3901 gagggtcgct tctgggccga ggatgcgaag catacccgac aagcgttccc accgttgccg
     3961 cgccgcgctc aggcgttgct tcgagcattc acccagccgc cgcccgattt cccgcagctc
     4021 ttgctttcgt gccattagtt cccgggcggg atggtgcgct ttcagggcaa gctgagcatt
     4081 cgtcaggcgg gttcgctccg tgatttggcg gcgttccacc gctcgccgga acgactcccg
     4141 ggaaaggtcg agctgttgtt gtgcgttgcg cagccgttgg ctcagttcgc ggaacaatac
     4201 cttatccgaa acgccgctca gccggccgcg ggcttgggcg aggaaattgc gccagcattt
     4261 ctcgagcgag tttttcaact cctgcatgcg tcgcgcgaga tcgcgcgcgt cggggacaat
     4321 caactcggcc gccgcgctcg gagtcggcgc gcgcaaatcc gccgcgaaat ccgcgatcgt
     4381 gaaatcgatt tcatgaccca ccgcggaaac gaccggaatg gcggaatgga aaatggcccg
     4441 cgctacgatc tcctcattga actcccaaag gtcttcgatg ctcccgccgc cacgcgtcac
     4501 cacgagcacg tcgagcgcct tccaggattc gttaggcgcg ttcagttcgc ggatcgcgct
     4561 ggcgatttcc agcgccgcgc cggtcccctg cacccgcacc ggactgatca aaacctcgac
     4621 gcccggcgcg cgccggcgca ggatattgag catgtcctga atggcagcgc cgctcggcga
     4681 ggtgatgatg ccgatgcgtc gcggaaattt tggcagcggc cgcttgcgcg ccgtttcgaa
     4741 aagaccttcc gcttccagtt tgcgcttcag cgcctcgaat ttcgcctgca acaagccgag
     4801 cccgcgcgac tgcaaaatct gcacgctcag ctgatactgg ccgcgcatct cgaagacggt
     4861 gatgttgccg tagacctgca cctgcatccc atcggccagc ggcgtggagg agggcggaag
     4921 ggtgtcgcga aaaatcacgc agttgagcgc ggcgcgctga tccttaagcg tgaaatactg
     4981 gtggcccgag ggatgtctct tgtaattgga aatctctccc tgcacccaga ccgcgccgaa
     5041 acgactcgcg agcagatcgc gaatcgcgcg ggtcagttcg gcgaccgtca gcaccctggc
     5101 gatgttcgga aagagatcag tgtccgaatt catgaggtgg cgcgggaatc tggggagcgc
     5161 acgcgactcg cgtgctggtt ttggcgaccc gccaaaacat tctttgctgc ctctcgatga
     5221 ccgagcagtg cggacagaaa gttcgcgatg gcgagtcgcc atcgccagca cgcgagtcgc
     5281 gtgcgctccc cggggttttg cggtcgcgca ttcttacgaa ccaaacaatt cgctctgcgg
     5341 tccggccggc gccttcaaac ccagttttcg gtaggcgtta ggcaacgcga cccgcccctg
     5401 cgcggtgcgt ttgatgtagc cttccatgat cagatacggc tcgttcactt cctccagcgt
     5461 gccggcttct tccccgaccg cgaccgccag cgagctcaag ccgacggggc cgccgttgaa
     5521 tttgtaaatc agcgtttcga tgatgcgctt gtcccattga tcgaatccgt cgcgatcgat
     5581 ctcaagcatc gtcagggcac ggtccgccag gtccgccgtg atgcgcccct cggctttcac
     5641 ctgcacgtaa tcgcgcaccc agcgcaggag attgttcgcg gtccgcggcg tgccgcgcgt
     5701 gcgggtcgcg atctccgccg cgcctgcggc atcgatttcg acgttcaaga gacccgcgct
     5761 gcgcaggatg attttctgca tctcttccgc gctataataa tcgagccgcg ccgtcatccc
     5821 gaagcgcgag cgcaacggcg cgctcaacat cccggagcgc gtcgtcgcgc cgatcagcgt
     5881 gaacttcggc aggcgcaatt gcaggctccg cgcctgcggt ccctggtcga tgatgatgtc
     5941 gagtttgtag tcttccattg ccgggtagag gtattcctcg atcgtcggct ggagacgatg
     6001 gatttcgtcg atgaaaagga cgtcgccttt ttccaggctg gtgaggaggc cagcgagctc
     6061 gcctgctttc tcgatcaccg ggccgctcgt gttcttgatg ttcacgccca tcgcgttggc
     6121 gatgatgtgg gcgagcgttg ttttcccgag gcccggcggg ccgctgagca ggatgtgctc
     6181 gagcacgtcg ccgcgtttct tcgccgcttc gaccagcacc gcgagccgct cacagacttt
     6241 cacctgaccg gtgaactcgc tgaacatcgg cggccggagc gaaatctcga aggccggatc
     6301 aggtgcggat ggaacgacgg caggcatcag aagaagttag acaggattta caggattatc
     6361 aggatgaaga atttccggaa ttccaaatcc tgctaatcct gtctcctctt actcgagatt
     6421 tttcgtcagc accaattccg ttccgcggct gcgcaggacg tagtcgactt gatcgaaggc
     6481 gttgcgaatc aagtacaggc cgaggccgcc cggcttgatc agatccggcg gtcggctttg
     6541 catcgcgttg ggcggcgcct gttttccata gtcgcgcagc cgcaggcaga cgcagtcgtt
     6601 cctgccctca agggaaagcg aaatcaagcg gtcgtcgcgc agttggtagg cgtgccggat
     6661 gatgttggtg caagcttcgt ccaccccgag gaccatgagg gtgcgctggc gctccgagaa
     6721 cgggaacgct tcgaggaagc gccgcacgca attgcgcatc agagcgagat tgccggtgtg
     6781 actggagaac tcgatttttt tcttcgtttt cttcatcgcc ccaaaaccgc cccacccacg
     6841 aaggagtttg tcaggcttgc aagctggcga agatagcgcg tcgtcgcaac gagtttccgc
     6901 tgggcggtgg ggcgagactc ggtcgagcag gaaatgacga atgacgaatg tctaatgacg
     6961 aaggaagggc gaaacccgaa tgaccagcac ttcgcggcgc cgccttcggg ttttggaatt
     7021 cgtcatttcc cccgttatgc gatcggcact ggcaatccag cgcgagccat gcactagttt
     7081 tgcatgtcga aatggttcat cgcgctgctc ttgctcgcca cgccgggctt cgcgcaggaa
     7141 cggcccacgg cttaccaggc cctgcgcacg atcggcattc agctgaagcg tgacctcgtc
     7201 aatcacgtta tctcggttac cgggaccaac ggcgatccgc agccgccaac atggaagatt
     7261 ttgctcgatg atccgcaggc ccggggcggc gtgcgggagg tggaagtggg caacggccag
     7321 attatctccg agcgcacccc gttgcggtct tcggtcgagg gctcgttagg cgcggtcatc
     7381 gatacctcga agctcaacct cgattcgagc ggagcctaca ccgtcgcgcg acagacggcc
     7441 gccaagtcgc acgtcacttt cgcgaccgcc gactacgccc tgcgggccga cgaacggggc
     7501 aatccgatgt ggatggtgac gctgcaaagc cagggcggcg agtcggccgg caagattttc
     7561 atcggcgcca atcacggcac gatcactcgc acggaggggt tcttcagcgg gggcgatcgg
     7621 accgcggtgg tcgacgagca aagcgacgag cccaatacga tggacgcgga tgacgccgac
     7681 gatggtgacc acaacatcgt cgagcgccgg gtccagcagg ctttccggga ggcgcgcgac
     7741 gatgtgaagc gcactttcca caaagtgcgg cgttcgttcg tcgatttttt ccaggacaaa
     7801 tgaatcgcca gatttcgtta gggcgaaact tcgtcgagcc gggggaagac attgcctgcc
     7861 caaacacccg ccatccgggc tcgcccgagt ctcgccccgc cgcttgcctg agcacatgcc
     7921 gttcagtctt gcattcagcg gcgcgcgccc gaaaatgacg cgatgaaagt cccgctcccc
     7981 gagcaaacag cctggaaaaa tgtcagccac ggctggcagc agctttatgg cagcttcgag
     8041 gatttgggga tcagcatcga atggcacgat ttccagacca gccagccgct tgattgggcg
     8101 gagagtttcc ggccgggcag catcgagttt tgtctcaacc tggaaggccg cggcgcgatc
     8161 ggttgccgga gcagcgcctc gcacgattac ggcccgagca gctccggcta ttacgcgatc
     8221 ggcgacgaac cgctccacgc gacccgccag gcccacgacc gccatcgttt cgtcacgttg
     8281 gaatattccc gcccctacct gcagacgcag gtcgcggaat ccgagagcga gctggccccg
     8341 gcgatgcgcg cggtgatttt tccgaagaag gaagagagca tcgtctcgcc gccgcaacca
     8401 atgtcggccg agcagcgcgc gattgtcgcc agcctgagcg agccgccggt gccgaaagcg
     8461 gcccgcatca tctggtatcg gagcaaggcg ctcgagctga tgtcgcattt ccttttcgag
     8521 gcgaaggatc cggaattctt ttgcatgcgg cagaagcggg tcgcccgcga ccgcgtgctg
     8581 cggacaaaag aactgctggt gcgcgacctg gcgaacccgc cgactctcga gatgctcggg
     8641 caggaggtcg ggtgcagccc gttttacctc agccggattt tttcgcgcga agttggcctg
     8701 accatcccgc agtttctgcg caacgcgcgg atggagcgcg ccgccgaatt gctccgcagc
     8761 ggccgctata atgtgaccga ggccgcgacg gaagtcggct actcgagcct gagccatttc
     8821 agcaaggctt tttgcgaaac gatcggctgc tgcccagtcc tctatccggc ggcgaaacac
     8881 atcgtgcttg atcgttaggc cggaatgagc gccgcggccg ccagcgccgg agccagacgc
     8941 tccggtcttt ttttcctccc caccgtgacg ctttggcagc gcgagatcgt gcgcttctat
     9001 cgccagcgca accgcatcat tggtgcgctc ctcacgccgt tcgttttttg gtttctcatc
     9061 ggctcgggga ttgggccgtc gttccgcgcc ggctcgtcgc ctaacggtgt cggctacctc
     9121 gaatattttt atccgggcac gctcatcctg atcatgcttt tcaccgccat cttctccacc
     9181 atttcaatca tcgaggatcg gcgcgagggt tttctccagt ccgtgctggt cgcgccgatt
     9241 tcgccgggga gcatcgtctg cgggaagatt ctcggcggca ccacgcttgc cctcatgcag
     9301 ggcttcattt ttcttttgct cgcgcccttt gtcggcattc acatcacgcc cgagcggctc
     9361 ctggtttgtc tcggcaccct cttcgttgtc ggatttggcc tgaccggtct cggttttctc
     9421 gtcgcctggc cgatggaatc gacccagggc tttcacgcca tcatgaacgt cttcctgatt
     9481 ccgctctggc ttatgtcggg cgcgcttttc cccgcgacca atccacactc ctggatcttc
     9541 tgggccatcc atctcaaccc ggtcacctac ggcgtcgcgg ccgtcaggcg cgcgctctac
     9601 gcggccgacc ccggggtcct ttacctgccg agcttcacgc tttccatcgg actctcggtc
     9661 gcttttggat tgatcatgta tgtgatcgct tacttcctcg tgcagcgaaa atcttcatga
     9721 aaatcaaacg cgctctcatt tccgtttccg acaaatccgg cgtggccgat ttcgcgcgcg
     9781 acctggaaaa gcagggcgtc gaaatcatct ccaccggcgg gacggccagc ctgctgcgcc
     9841 aggaaggcat caagacgcgc gacatctcca gcttcaccgg ctttcccgaa attctcgacg
     9901 gccgcctgaa aacgctgcac ccgaaagtgc acggcgcgct tctctacctg cgcggcaaca
     9961 acgagcacga agccgccgcg cgcgagcacg ggatcgagac gatcgatctc ctcgcggtca
    10021 atctttatcc cttcgaagcc acgatcgcga agcccggcgt caacctggcc gcggcgatcg
    10081 agcagatcga catcggcggg cccgcgatgt tgcgcagcgc ctcgaaaaat tatcagtcgg
    10141 tcacggtggt ggtcgatccg gcggactacc agtcggtgct cgacgcgatg cacgatcgcg
    10201 aaggcgaaac gacgctgaaa ttgcgcgagc gcctggcgat caaggctttc ctcaaaacct
    10261 ccgattacga tcgcgcgatc gcgagctacc tgaatgagaa acaggaaacc gaaacctcgt
    10321 tttccctttc gttgccgctc gtcagccgct tgcgctacgg cgagaatccg caccagacgg
    10381 cgtcgcttta cgggacgttc gcggaccatt tcgaaaaatt gcatggcaag gacctttcct
    10441 ttaacaacct gctcgacatc acggccgcgg ccgacttgat cgatgagttt tctgagccga
    10501 cggtcgcgat cctcaagcac accaacccgt gcggcgtggg gaccgacccg gatctgcggg
    10561 aagcgtggct gaaagcgttc gcgaccgaca agcaggcgcc tttcggcggc atcattatct
    10621 gcaaccggcc gctcgatctt ccggtggcca aggcgatcag cgaaatcttc agcgaagtga
    10681 tcatcgcgcc ggagttcgcg actgacgccc gcgccctgct ccagaaaaag aaaaacctgc
    10741 gcttgatgcg gacgttgcaa ccggaagtgc ctaacgaaaa gccggcgatg gatgttcgtt
    10801 ccgtgcgcgg cggtttgctc gtgcagcaaa aggacgcggc cgaactcgaa ggtctcgaaa
    10861 cgaaagtcgt gaccaaacgt ccgccaacca agcaggagat ggccgcgatg cttttcggct
    10921 ggcgggtggt gaaacacgtg aaatcaaacg cgatcgttta cgcgagcgcg gatcgtaccc
    10981 tggggatcgg cgccggccag atgtcgcgcg tcgacgcctc gcgcatcgcg gtctggaagg
    11041 cggccgagtc aggcctgtcg ctcaagggca gcgcggtcgc gagcgacgcc ttcttccctt
    11101 tcgcggatgg cttggtcgcc gccgccgaag cgggcgcgac ggccgcgatt cagcccggtg
    11161 gctcggtgcg cgacgaagaa gcgatcgccg ccgccgacga acgcgggatg gcaatggtct
    11221 tcaccggcgt gcggcatttc cggcactaga ggcgactacc gcgcgggtgc tcctgttgcc
    11281 ttgtaataca cctttttaac ccaaagatga cgtttccggc gttttggttt tgtctcgccc
    11341 tgataggcat ggtattgggc gcgtttttct ttcggcggtt gcggttagaa tgttacgagg
    11401 aatggtgccg catcggtagc ccaagcgctt ttcgcgtcaa caataacgca aagcaaagct
    11461 ggtttataat gcggcatatt ttagccggtg attttgaaca tctttcgaac agacgcgtcg
    11521 ttaggctcgg tcgagtattg cgttactacc tctggctgta tatcattgct ttttgcgcgt
    11581 cgatggcctt gctcgtgttt gtcttggtca cataagcaat ggtcgttgtt atggctgccg
    11641 caaggcatcg aaacaaagaa ggcgtagaat cagccttctt ttttggacct cttgcttcgg
    11701 cggaataagc ccgtcaatcc ggggtcagtc ctaaggaagt aatcatttct cgtatgcagt
    11761 agacggctca actgagttct ttcggaacag caacgcccct gtaaccaact cctcccggta
    11821 aagcgccttc aaaaaagtgc gcgctggcgg gacgccagcg ccagcacccg aggcgggtgc
    11881 gctacccaga ctgacgagtg caaagggaag cgtctatggg tcaggctatc cgccggtggc
    11941 gccggaacat cgcggattgc acgacgatgc cgaagatagt tagtgccacg acgagaaccg
    12001 ttgtgccggt gctcgggaga tgaatgctgc tcgccagcaa gcgtgcgcct tcgatcgcgg
    12061 agaaaataat cagcgcccaa tcgaagagtc caagaaggag aaacgcgccg aggattccgc
    12121 cgacgacgaa agtgatgatg gagtaatgcg catggttcgc gatgaacgcc gccgcgacgg
    12181 cgatcgcgac gcgtcc
//
LOCUS       DATMNS010000193         8641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5277001, whole genome shotgun sequence.
ACCESSION   DATMNS010000193 DATMNS010000000
VERSION     DATMNS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5277001"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /gene="nusA"
                     /locus_tag="VNW28_06570"
     CDS             <1..1153
                     /gene="nusA"
                     /locus_tag="VNW28_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXA09628.1"
                     /translation="ASKKSVSASRDLHIDIDPKTGEIRALANLLVVETVTNPQDEIAL
                     DKARRIKPDAVVGEAVEVEVTPKNFGRIAAQTAKQAMMQRIRQVEKEMIYEEFKDRAG
                     EIVSGTVRRFDRSDVILDLGKFEAIMPQRERVVVEDYNVGDRLRAYVVAVENGIRGPE
                     IIISRSHPNFVRRLFELEVSEIADGTVEIRGIAREAGYRTKIAVFSDNPKVDPVGACV
                     GMRGSRVKNIVRELNNEKVDIIRWSSDPKEFVLEALKPAKVKNLVFDLEKKSATISVD
                     EDQLSLAIGKKGQNARLTSRLTGWEINIEKEAPSTTAVEQKVAHAAQTLATSLPITEE
                     QAMTLVKMGFTNLEGLRDAELADLVDILGVEEEKAREIYEAVHREAVSS"
     gene            1533..3551
                     /gene="infB"
                     /locus_tag="VNW28_06575"
     CDS             1533..3551
                     /gene="infB"
                     /locus_tag="VNW28_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXA09629.1"
                     /translation="MAPEPPKPVVESVSLIDPHTPKPKRVPSDDAVKRTILPPISRIR
                     ATPAAPEPAREAVVEIPAAAPSRAADAEVETETEDGRKIIHIKPPIIVKDLAAQLGLK
                     NFQLIRELMDDFNIFANPNLTVEPEVATKVCEKHGFVFEMERREKGGGVHKIEQVVVA
                     PPPPVIEKEEELKLRAPIITFMGHVDHGKTSLMDAIRKTRVAAGEAGGITQHIGAYTV
                     DHKGTRITFLDTPGHAAFTAMRARGANVTDIVVLVVAADDGLMPQTIEAIHHAKAAPH
                     VKIMVAINKIDLPSANIDRAKQQLQEHDLTPEDWGGDTIVVPVSATKGIGVEELLENM
                     VAQAEVMELKASPTATPRGTVIEAQVEAGRGPTATVIVQMGTLKVGEPFICGSYGGKV
                     KSLVDDQGETIKSAGPSTPVKVLGFTGLPNAGDEFLVMESERSAKLLSEERLATKRAE
                     KLSMPQRATLESLLEAADGKKVLRLVLKCDAQGSLEALTASLGQIQSKKIDLEMIHSG
                     VGPISESDILLASASNAVVVGFNVKVESNAVGAAKREGVQVKLYSIIYELIDQIKEAM
                     AGLLDPEHRETVIGHAEVKQVFQLSRGIVAGCLVTDGRIARTARARVLRRRQPVYDGG
                     LSTLRRFQDDVKEVRVGLECGIKLGDFNEYQVGDVIECYQLEQVAQKL"
     gene            3611..3964
                     /gene="rbfA"
                     /locus_tag="VNW28_06580"
     CDS             3611..3964
                     /gene="rbfA"
                     /locus_tag="VNW28_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXA09630.1"
                     /translation="MKHRLLRVNELLKRELSGLLVREMTFENLLVTVNQVDVTPDLKS
                     AHVYISVLGSKGRKEVLPKLEASRTALQADLSKHVVLKYTPHLVFHLDDSIERGSRVL
                     EIMQEIEPPPNAANE"
     gene            3961..5001
                     /locus_tag="VNW28_06585"
     CDS             3961..5001
                     /locus_tag="VNW28_06585"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HXA09631.1"
                     /translation="MNVTTAEPAAETAASPPAALTNSTFAEIAEVLRREERFVVLSHV
                     RPDGDALGSQIAMGLALQKLGRKVKVWNEEGFLEKFSFLPGGELVSLPPNEPEDFDVV
                     LALDTATRQRLGENCLAAIGRAKIWINIDHHPSNPRYGDLVHIDPLAPATGQILFELL
                     QSQDLPLDEPIAENLFAAISTDTGSFQYPNTTARTFEIAADILRCGIKVGELSQKLYE
                     SYPRRRIELLRVLLDRMRFDADGKVASFSLPLATANELGVIPEDNEGLIDHLRAIQGV
                     IIAIFFEELEDGKVRVSMRSKDERADVCEICLRFKGGGHKLAAGARVPGALEEVASRV
                     MEAACDVVNRLP"
     gene            5089..5811
                     /gene="truB"
                     /locus_tag="VNW28_06590"
     CDS             5089..5811
                     /gene="truB"
                     /locus_tag="VNW28_06590"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXA09632.1"
                     /translation="MALTATPDGVLLVDKAAGMTSHDVVALVRRQLQIKKVGHCGTLD
                     PIATGLLILTLGRGTKIQDLLMSEDKEYVGTLSLGKTTDTQDRAGQVTAERPVPELTG
                     ENVRAAFEKFCGDFYQMPPMVSAIKQGGVPLYKLARQGKVVEREPRLVHVYRYSIDRV
                     ASPEIDFTVLCSKGFYVRTYAHDIGEMLGCGAHLQELRRTRSGRFDLARAVTVEEIKT
                     ADPVDILSKILTLPEVSRMRGA"
     gene            5825..6781
                     /locus_tag="VNW28_06595"
     CDS             5825..6781
                     /locus_tag="VNW28_06595"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HXA09633.1"
                     /translation="MRILRSIPELAQIPGPVFLAIGVFDGVHLGHQAVIGSTVRHAGE
                     AGGTAVVVTFDPHPAKFLRPQESPRLLTATDHKIALLRELGVSHLLVLTFDQRLASTP
                     PEEFVRQLVAASKPLREICVGQEWSFGKNRAGNLALLKKLGVEMNFHVVGVVPVTRDG
                     VIVSSTAIRRAVESGDLALAAKMLGREYTILGTVEEGNRLGRSLGFPTANLSAHSEQF
                     PPNGVYAAEGRLDGRVMRGVVNLGVRPTVATDAPQRVLEFHLFDFDRDLYGVDIEVRF
                     LRYLRPERKFENLAALREQIARDTTEARQIFAAEAGGEVSPA"
     gene            complement(6778..7131)
                     /locus_tag="VNW28_06600"
     CDS             complement(6778..7131)
                     /locus_tag="VNW28_06600"
                     /inference="COORDINATES: protein motif:HMM:NF027794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5056 domain-containing protein"
                     /protein_id="HXA09634.1"
                     /translation="MGTELTMDEQLQEDWLDARLRQEMPYIDDDGFTARVVQKLPAPA
                     RRRSFRETILICVTLLASVVTYFVSDGGRFLVTAFYRLASMPVLFVGLVAICCALVLS
                     AVAAGAALSNVREQR"
     gene            complement(7116..7652)
                     /locus_tag="VNW28_06605"
     CDS             complement(7116..7652)
                     /locus_tag="VNW28_06605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXA09635.1"
                     /translation="MPLNDADLVARVLLEDDHHAFAELVRNHQSAVRGLLRQLTRSDF
                     ALADDLAQETFLRAYKNIRSFRGEAKFSTWLYRIAYNCFREEARKRKELVGIDEARLE
                     AEQDPQTVDPALKHDLMHALQLLPLPERSAILLCCQNGLSHDEAARVLDIPLGTVKTN
                     VLRGREKLKKTLAAWGPN"
     gene            complement(7662..8363)
                     /locus_tag="VNW28_06610"
     CDS             complement(7662..8363)
                     /locus_tag="VNW28_06610"
                     /inference="COORDINATES: protein motif:HMM:NF039701.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6249 domain-containing protein"
                     /protein_id="HXA09636.1"
                     /translation="MKITFLKIKLFTYFCCVVALACSAHGQSPTVPPGTPVAAMTPAA
                     SVISAAASPVVPVTSATAAPAVAPGASPDSDEDFDKAIEKKIRKHFNFDFDRGHSGEH
                     SEDDVPWIAVPIVLIVFLSIFGVPVVIVGLIMYFSFSKSRALHKTVRLMVEKGQPVPE
                     ALLNPPPVVRQRSDLRRGVVLLMVGAGLMVFFGAVNDWEGGVWSLGIIPFLIGLGYLL
                     VWRLDVHREDPSPKV"
     gene            complement(8444..>8641)
                     /locus_tag="VNW28_06615"
     CDS             complement(8444..>8641)
                     /locus_tag="VNW28_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09637.1"
                     /translation="NGSTLISNDDWQQTQEMEISQTGLAPNDIRESALAISLAKGNYT
                     GIARGKSDATGIAVVEVYNVL"
BASE COUNT         1812 a         2524 c         2709 g         1596 t
ORIGIN      
        1 cgcttccaag aaaagtgtca gtgcctcgcg cgatttgcat atcgatatcg atccgaagac
       61 cggcgaaatt cgcgccctcg cgaatctgct cgtggtcgaa acggtgacca acccgcagga
      121 cgaaatcgcg ctcgataagg cgcgccggat caaacccgac gcggtggtgg gcgaagcggt
      181 cgaagtggag gtgaccccga aaaatttcgg gcggatcgcc gcccagaccg ccaagcaggc
      241 gatgatgcag cgaatccggc aggttgagaa ggagatgatc tacgaggaat ttaaggatcg
      301 cgcgggcgag atcgtgagcg gcacggtgcg acgcttcgac cggtccgacg tgattctcga
      361 tctcggaaaa tttgaagcga tcatgccgca gcgcgagcgg gtcgtggtcg aggactacaa
      421 tgtgggggat cggttgcgcg cctacgtcgt cgcggtcgag aacggcattc gcgggccgga
      481 aatcatcatc tcgcgcagcc atccgaattt cgttaggcga ctcttcgaac tcgaggtgag
      541 cgagatcgcg gacggcacgg tcgaaattcg cgggattgcg cgggaagccg gttatcggac
      601 gaagatcgcc gtcttcagtg acaatccgaa agtcgatcca gtcggtgctt gcgtcgggat
      661 gcgcggttcg cgagtgaaaa acatcgtgcg cgagttgaac aacgagaagg tcgatatcat
      721 tcgctggagc tccgatccga aggaatttgt cctcgaggcc ctcaagccgg cgaaggttaa
      781 gaatctcgtt ttcgatctgg agaagaagag cgcgaccatt tcggtcgatg aagatcagct
      841 ctctctcgcg atcgggaaaa aagggcagaa cgcccgtctc acgtcgcgtc tgaccggctg
      901 ggagattaac atcgaaaaag aggcgcccag caccacggcg gtggaacaaa aagtggccca
      961 cgccgcgcaa acgctcgcca cttcgttgcc catcaccgag gagcaggcga tgaccctggt
     1021 gaaaatgggc tttaccaatt tggaaggatt gcgcgacgct gaactggccg atctggttga
     1081 tattcttggc gtggaagaag agaaggcgcg cgaaatttac gaggcagtgc accgggaagc
     1141 cgtctcttcg taaaatggcc gcgcagcgag accgctcacg gccatgagca gatttttgag
     1201 gaacattgat gccaacaaaa acgacgacca aaaccacggc gcccaaagcg ccggtccgca
     1261 aagcggcgaa gtccgctgcg cctgctaagt cgtcgattcg ttcccgtttg gcggccaagc
     1321 ccgcaaaaac atccaaggcg cccgggaaag aagaagtcgc cgccgttcca gagtccgacg
     1381 gggcagcagt ttcggtcgcg gtcgaacggg agggggccgg cgtgccgtca aagcagttgg
     1441 cgtctccagg caaacgggag gttgttgcac cgaaatcggc ttttgcccca attacggcga
     1501 aaaaacaacc ggccaagccc gccccagttg ctatggcacc ggaaccgccg aagccggtcg
     1561 tggaaagcgt ttcgctgatt gatccacaca ccccgaaacc gaagcgtgtt ccgtccgacg
     1621 acgcggtcaa gcgcaccatt cttccgccga tttcccgcat ccgtgcgacc ccggctgcgc
     1681 cagagccggc gcgcgaggcg gtggttgaaa taccggccgc cgctccgtcc agggcggccg
     1741 acgccgaagt cgagaccgaa accgaggacg gccgcaagat cattcacatc aagccgccga
     1801 ttatcgtcaa ggatctcgcg gcgcagctcg ggctgaagaa ttttcagctc atccgcgagc
     1861 tgatggacga cttcaacatt ttcgcgaatc ccaacctgac ggtggaaccc gaagtggcga
     1921 cgaaagtttg cgagaagcac ggcttcgtct ttgagatgga acgccgcgaa aagggtggcg
     1981 gcgtgcacaa gatcgagcag gtggtggtcg cgccgccgcc gccagtcatc gaaaaggaag
     2041 aagagcttaa gctgcgcgcg ccgatcatca cgttcatggg gcacgtcgac cacggcaaaa
     2101 cgtcgctcat ggacgcgatc cgcaagacgc gcgtcgcagc gggtgaagcc ggcggcatca
     2161 cccagcacat cggcgcttac acggtcgatc acaaagggac ccgcatcacc tttctcgaca
     2221 cccccgggca cgccgccttc accgcgatgc gcgcgcgggg cgcgaacgtg accgatatcg
     2281 tcgtgctcgt cgtcgcggcc gatgacggct tgatgccgca aacgatcgaa gcgattcatc
     2341 acgctaaggc cgcgccacat gtgaagatca tggtggcgat taacaagatc gatttgccgt
     2401 cggcgaatat cgaccgcgcc aagcaacagc tccaggagca cgacctcacg cccgaggatt
     2461 ggggcggcga cacgatcgtc gtgccggttt ccgcgaccaa ggggatcggc gtcgaagagt
     2521 tgctcgagaa catggtggcc caggcggaag tgatggagct gaaagcttcg ccgaccgcga
     2581 ccccgcgcgg cactgtgatc gaagcgcagg tggaggcggg ccgcggcccg accgcgacgg
     2641 tcatcgtgca aatgggcacg ctcaaggttg gtgagccttt tatttgcggc agctacggcg
     2701 gcaaggtgaa atcgttggtc gacgatcagg gcgaaacgat caagtccgcg ggtccctcga
     2761 ctccggtgaa ggtcctcggc tttaccggtt tgcctaacgc cggcgacgaa ttcctcgtta
     2821 tggaatcgga aaggtccgcc aagctcttga gtgaggaacg gctggccacg aagcgcgccg
     2881 agaaattgag catgccgcag cgcgccacgc tcgagagtct gctcgaagcc gccgacggca
     2941 agaaagtgtt gcgcctcgtt ttgaagtgcg acgcccaggg ttcgctcgag gcgttgaccg
     3001 catcgttagg ccagatccag agcaagaaga tcgatctgga aatgattcac tccggggtcg
     3061 ggccgatttc cgaatccgac atcttgctgg ccagcgcctc gaacgccgtc gtggtcggat
     3121 tcaacgtcaa ggtcgaaagc aacgccgtcg gggcggcgaa acgcgaaggc gtgcaagtca
     3181 aactttacag catcatttac gagctgattg accagatcaa agaagcgatg gcaggtctgc
     3241 tcgatccgga gcatcgcgag acggtcatcg gccacgccga ggtgaagcag gttttccaat
     3301 taagccgcgg gattgtcgcc ggctgcctcg tgaccgatgg gcgcatcgcg cggaccgcgc
     3361 gcgcccgtgt gctgcgccgg cgccaaccgg tttacgatgg cggtctctcc acccttcgcc
     3421 gttttcagga cgacgtgaag gaagtgcgcg tcggtctgga gtgcggtatc aagctggggg
     3481 acttcaacga atatcaagtc ggcgacgtga tcgagtgtta ccaactcgaa caggttgcgc
     3541 agaaactgta gccgggggcg ctaaccccgg ccggcgtcag ccccgccggc tacaacttgc
     3601 gtcactacca atgaaacacc gacttttgcg cgtcaatgaa ctcctgaaac gggagttgag
     3661 cggcctcctc gtgcgcgaga tgactttcga aaatttgctc gtcacggtga accaggtgga
     3721 cgtcacgccc gatctcaaat ccgcccacgt ctacatcagc gtgctcggtt ccaagggccg
     3781 caaggaagtc ctcccgaaac tggaagcgag tcgaaccgcg ctccaggccg acctttcaaa
     3841 acacgtcgtg ctgaaataca ccccgcacct tgtttttcac ctcgacgatt cgatcgaacg
     3901 cggctcccgc gtcctcgaaa tcatgcagga aatcgaacct ccgcccaacg ccgccaacga
     3961 atgaacgtca ccactgccga gccggcggcc gagaccgccg cctctccacc cgctgcccta
     4021 acgaactcaa cctttgcgga aatcgcggag gtcctgcgcc gggaggaacg cttcgtggtg
     4081 ctgagccatg tccggccgga cggggacgcc ctcggcagcc agatcgcgat gggcctcgcg
     4141 ctgcaaaagc tgggccggaa ggtgaaagtt tggaacgaag agggattcct cgaaaaattt
     4201 tcctttctcc cgggcggtga attggtttct ctcccgccta acgaaccgga ggatttcgac
     4261 gtcgttctcg cactcgacac cgcgacccgg cagcgcctcg gtgagaattg cctggcggcc
     4321 atcggccgcg ccaagatctg gatcaacatc gatcatcacc cgagcaaccc gcgttacggc
     4381 gatttggtgc acatcgaccc gctggctccg gctaccggtc aaattctttt tgaactgctc
     4441 cagagccagg atttgccgct cgacgaaccg atcgcggaga atctctttgc cgcgatttcc
     4501 actgacacgg gttcattcca gtatcccaat acgaccgccc gcacttttga gatcgcggcc
     4561 gacattctcc gctgcggaat caaagtgggc gagctgagcc aaaagcttta cgaaagctat
     4621 ccgcgccgcc ggatcgagct gttgcgcgtg ctgctcgatc ggatgcgttt cgacgccgac
     4681 ggcaaagtgg ccagctttag cctgccgctg gccacggcta acgaactcgg cgtcatcccc
     4741 gaggataacg aaggcttgat cgatcatctg cgcgcgatcc agggtgtgat tatcgcgatc
     4801 tttttcgaag aactggaaga cggcaaggtg cgcgtcagca tgcgctcgaa ggacgagcgc
     4861 gccgacgttt gcgagatctg tcttcgcttt aagggcgggg gtcacaaact ggcggccggc
     4921 gcgcgggtgc cgggagcgct ggaggaagtg gcgagtcgcg tgatggaagc tgcttgtgat
     4981 gttgttaacc gcctccccta actcggaggg acgtgcctcc gcacgtccag gacatgccga
     5041 agcatgcccc tccggagatt gttgtccgcg accacgattc gttttcccat ggcactaacc
     5101 gccacacccg acggagtcct gctcgtcgat aaagccgcgg ggatgacgtc ccacgatgtt
     5161 gtcgcgctcg ttaggcggca gctccagatc aagaaggtcg gtcattgcgg gacgctcgat
     5221 ccgatcgcca ccgggctgct cattctcacc ctcggccgcg ggacgaaaat ccaggatctg
     5281 ctgatgagcg aggataagga atacgtcggc acactgtcgt taggcaaaac gaccgacacc
     5341 caggatcgcg ccggtcaggt gaccgcagag cggcccgtac cggagttgac cggggaaaat
     5401 gtccgcgcgg ccttcgagaa attctgcggc gatttttacc agatgccacc gatggtttcg
     5461 gcaatcaagc agggcggcgt gccactttac aaactggcgc gccagggaaa ggtggtcgag
     5521 cgcgagccgc gcctcgtgca cgtttatcgc tacagcatcg atcgcgtcgc ttcgcccgag
     5581 atcgatttta cggttttgtg cagcaaaggt ttttacgtcc gcacctacgc gcatgacatt
     5641 ggcgaaatgc tcggctgcgg cgcgcattta caggagctgc ggcgcacgcg ttccggccga
     5701 tttgatctgg cccgcgccgt gacggtggag gagatcaaga ccgctgatcc ggtcgatatt
     5761 ctctcgaaaa ttctaaccct ccccgaagtc tcccgaatgc gcggtgccta acgacggttc
     5821 taacatgcgc atcctgcgtt ccattccgga gctggcgcaa atccccgggc cggtctttct
     5881 tgccatcggc gtcttcgacg gggttcatct cgggcatcag gcggtcattg gcagcaccgt
     5941 tcgtcatgct ggggaagccg gcggcacggc ggtcgttgtc acctttgatc cgcacccggc
     6001 gaaatttttg cggccccagg aaagcccgcg gctgctgacg gcgacggacc acaagatcgc
     6061 gctcctgcgc gagctcgggg tttcgcattt gcttgtcctg accttcgatc agcggctcgc
     6121 ttcgactccg cccgaagaat tcgttaggca actggtcgcg gcctcaaaac cactgcgcga
     6181 aatctgcgtc ggccaggaat ggtctttcgg caagaatcgt gccggcaacc tggcgttgct
     6241 gaaaaagctg ggcgtggaaa tgaatttcca tgtcgtcggc gtggtaccgg ttacgcgcga
     6301 cggcgtgatc gtgagcagca cggcgattcg ccgcgcggta gagagcggag acctggcgct
     6361 ggcggcgaaa atgctcgggc gcgaatacac gattctgggg acggtggagg aggggaaccg
     6421 tctcggccgc agtctcggct ttccgacggc gaacctgagc gcgcacagcg agcagtttcc
     6481 gccgaatggc gtttacgcgg cggaaggccg gctcgacgga agagtcatgc gtggggtggt
     6541 taatcttggg gtgcgcccca cggtggcgac ggacgctccg cagcgcgtgc tcgagtttca
     6601 tcttttcgat ttcgatcgcg atctttacgg ggtagacatc gaggtgcggt ttttgcgtta
     6661 cctgcggccg gagcggaagt tcgaaaacct ggccgcgttg cgtgaacaga ttgcgcggga
     6721 cacaaccgaa gcgcgccaga ttttcgcggc ggaagcgggt ggcgaggtga gtcccgccta
     6781 gcgttgctcg cggacgttag agagcgccgc gccggcggcg accgcgctca gcacgagcgc
     6841 gcagcaaatc gccaccagac caacgaagag cacgggcatc gaggcgagcc gataaaacgc
     6901 ggtgacgaga aagcggccgc cgtcggaaac gaagtaagtg accacgctgg cgagcagcgt
     6961 cacacaaatc aggatcgttt cgcggaatga gcgacgtctg gcgggggcgg gcaatttctg
     7021 cacgacccgc gcggtgaagc cgtcatcatc gatatacggc atctcctgcc gcagtcgggc
     7081 gtcgagccag tcttcctgga gttgttcgtc catcgtcagt tcggtcccca cgcggcgagc
     7141 gtctttttca atttctcccg gccacgcaag acgttggtct tcacggtgcc gagcggaata
     7201 tcgagaacgc gcgctgcttc atcatgcgat aaaccattct ggcagcaaag caaaatcgcc
     7261 gaccgctcgg gcaggggaag aagttggaga gcgtgcatga gatcgtgttt cagggcgggg
     7321 tcgacggttt gcggatcctg ctcggcctcg agtcgcgcct cgtcgattcc gaccagttcc
     7381 tttcgtttgc gggcctcctc gcggaagcag ttataggcga tgcgataaag ccaggtggaa
     7441 aatttcgcct cgccgcgaaa gctgcggatg tttttgtaag cgcgcagaaa agtttcctgg
     7501 gcgagatcgt cggccagggc aaagtcgctg cgcgtgagct gccgcaggag accacgcacc
     7561 gcggactggt ggttgcgcac cagttccgcg aaggcgtggt gatcatcctc cagtagaacg
     7621 cgtgcgacaa ggtcggcatc attgagcggc acgcagggag tttacacctt gggggaaggg
     7681 tcttccctgt ggacgtcgag tctccagacg agcaggtagc ccaagccgat gagaaacgga
     7741 atgatgccca ggctccagac gccgccttcc cagtcgttga cggcgccgaa gaagaccatc
     7801 aaacccgcgc cgaccatcag gaggacgacg ccgcgacgca aatcggagcg ctgtcggaca
     7861 acgggtggcg gattcaagag ggcctcggga actggctggc ctttctcgac cattaaccgg
     7921 accgttttgt gaagggcgcg gctctttgaa aagctgaaat acatgatcag gcccacgatc
     7981 acgaccggaa cgccgaaaat ggaaagaaac acgattaaca cgatcgggac cgcgatccac
     8041 gggacatcat cttcggagtg ctcacccgag tgaccgcgat cgaaatcgaa attgaagtgc
     8101 ttgcggatct ttttctcgat cgctttgtcg aaatcctcgt cgctgtcagg gctagccccg
     8161 ggagcgacag ctggggcggc ggttgcgctt gtgacgggca ctacgggcga agcggcggcc
     8221 gagatgacac tggcggcagg cgtcatcgcc gccaccgggg tcccgggcgg aacggttggg
     8281 ctttgaccgt gcgcggagca ggcgagagcg acgacacagc aaaagtaagt gaagagtttg
     8341 atcttgagga aggtgatttt catatttgtc ctttgctttg agatgcaacg ggcatgcctt
     8401 ttggattcaa cattttgagg tagcgcgaga tcccaggtgc cggctacagc acgttgtaga
     8461 cttcgacgac cgctattccc gtcgcgtcac tcttgccgcg cgcgatcccg gtgtaattgc
     8521 ctttggctag tgaaatcgcc agcgccgact cacgaatgtc attcggcgcc agaccggttt
     8581 ggctaatttc catttcctgc gtctgctgcc agtcatcgtt actgatcaac gtggaaccgt
     8641 t
//
LOCUS       DATMNS010000194         5359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3357955, whole genome shotgun sequence.
ACCESSION   DATMNS010000194 DATMNS010000000
VERSION     DATMNS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5359
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3357955"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VNW28_06620"
     CDS             <1..317
                     /locus_tag="VNW28_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXA09638.1"
                     /translation="ATIGPLVGLFGTVIGIIRSFGALGSELGPMRYVLLSKGISEALI
                     NTCGGLGIGITAMIFYAFFRGRAQRLVSDLESASTHIVALLSLQQSPRRERASALLDD
                     EF"
     gene            367..774
                     /locus_tag="VNW28_06625"
     CDS             367..774
                     /locus_tag="VNW28_06625"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXA09639.1"
                     /translation="MNFRSRTASPHPGIQLAPLVDVLLLLLIFFLMTWNAARNENELD
                     VKVPKASAAKEKTAPIGDVIVNVKSNGNVVVNRRTLSEPDLTTMLQGLVKLNPDQAVV
                     IRGDEAGAYKNIVNVLNICSQAGVTNVAFATAK"
     gene            771..3296
                     /locus_tag="VNW28_06630"
     CDS             771..3296
                     /locus_tag="VNW28_06630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA09640.1"
                     /translation="MKPRRRAATLLVAAAVLLLRREAPAQTPTPIRRAIPVQEPPVAR
                     ALPVATPPPATAPTIMRALPVPVETEPVAPSFTPSQSSAGGPPPESAEPPEQRQLNYA
                     NALFGRKLYDLAVPEYEKFLGLYPDSPNRATALFYLGEAYRALGRTPAARTSFQSVLQ
                     DFPESDLAGPASYGLAEIAFNEKDYASALPLFHRAAAKVKASELALSARYFEGRCLEN
                     LERRDEARDVYQQVIEVNNPNPYRDDSRFAVALIFLTNGRKADALKQFEALAEETSKP
                     SLKAEATVRAGLVAVDLVQSDKGKPDKAMIAKATALLQKGRALPGARRWSGIAGVGLL
                     RLEYQAGQYAQAVADYQRSKEQVPEEVLPEMMLLAANSQRQLSHFNEAQDLYRQIMQK
                     YPGREEAKDARYQHLIGLYNANDPALRDEIDDFIRDNPESERGDQAKLLKAEALYKEH
                     DFAAAALLYTALRDSKLSAKLRAESAFKLGWCFVQTKELANVIDAFAYFLQAFPDNPQ
                     VPSALAQRALAYQEDKQYESARADLDRLLKNYPEAREREAALQQKALILGQTDDALGM
                     TATFQQLLKEFPKSAAAAQAHYYIGKSAFEAKDYERAIAEMNKARQLNPEQYRTPAAL
                     RIMSSYFYLKQRAALAKEVNQFYAASPDGQVPAEILEWLGIDFYNARDYAAAAKYLTA
                     LSKAAIVSSVKPDFWFYLGDAEMKLNQPAEAEVALQKFLETVSDPAAKAKGLLALGAA
                     KIAAHKPDDAQKIADEIMTLQPEGRTNAQARLLAGDVEMERQQFEDAGKAFMSVALLY
                     DDPEITPQALAKAAKAYEKAGKLDEAQRVNAQLHQKYPDYAGG"
     gene            complement(3478..4008)
                     /locus_tag="VNW28_06635"
     CDS             complement(3478..4008)
                     /locus_tag="VNW28_06635"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09641.1"
                     /translation="MFAALYLPDFELQAALRHEPELQRKPLALVDDETAKATIMQLTP
                     GAAEAGVSAGMTPSQGLARCLRLLLKPRARAQEKIAGEILLHHAATLAPEIEATAPGV
                     CTVRFTSARNCRENIERVVRQLARLELTAQAGLAATPDLSFLAACLARPILQIDDPQK
                     FLGPLPIETLLHLPWS"
     gene            complement(4008..4688)
                     /locus_tag="VNW28_06640"
     CDS             complement(4008..4688)
                     /locus_tag="VNW28_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09642.1"
                     /translation="MAGFGMAASRIIDLRKLLAERFPQEALPPVDRLVTGLRLFDEAL
                     GGGLTKGAITELSSAPPSAGSASIIAALLQRACGERYFLALIDGRDSFAPPSLGNAAL
                     RHLLWVRCRKASEAMQAADLLLRDGNFPLVILDLVLNPLTELSRIPTSNWYRLQRLVE
                     PAPTAFLVFTPRSMISSAQWKLVLENRWTLLQLDVAVADLQAQLKIRLQRAQPNKWRS
                     DPKIARAG"
     gene            complement(4728..>5359)
                     /gene="lexA"
                     /locus_tag="VNW28_06645"
     CDS             complement(4728..>5359)
                     /gene="lexA"
                     /locus_tag="VNW28_06645"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HXA09643.1"
                     /translation="GFRQHVHMLTPGQKNVLQFIQAEQRTKGLTPSTREIQEHFGFAS
                     QTSVMQYIAALERKGFLQRHARKARALITAAPQSRVVDIPIYGEIPAGMATLAEQNIE
                     GHVALDLESLNASRSGRTFALRVRGDSMIGAHILDGDIVILEDRQSARNGDIVAALID
                     GETTLKRYVTERGRPYLKAENPRFPDLVPARELRIQGVMTSLVRKQSPG"
BASE COUNT         1094 a         1719 c         1590 g          956 t
ORIGIN      
        1 tcgcgacgat cgggccgctc gttggtttgt tcgggaccgt gatcggaatc atccgctcgt
       61 ttggcgcgct cggctccgaa ctcggtccga tgcgctatgt cctgctttcg aaagggatca
      121 gcgaagcgct catcaacacc tgcggcggtc tcgggatcgg catcaccgcg atgatcttct
      181 acgccttttt tcgggggcgt gcgcagcggc tcgtctccga tctcgaatct gccagcaccc
      241 acatcgtggc tctcctctcg ctccagcaaa gcccgcgccg ggaacgcgct tcggcgctgc
      301 tcgacgatga attttgagcg gcgttaggcg tcgaaacttt tgcgtccctc ccgggcgttg
      361 actcagatga acttccgcag ccgcaccgct tcaccccatc cgggtattca gcttgcgccc
      421 ctggtcgatg tcctcctgct gctcctcatc ttttttctca tgacctggaa tgccgcgcgc
      481 aacgaaaacg aactcgacgt gaaggtgccc aaagcaagcg ccgcgaagga aaagacggcc
      541 ccgatcggcg acgtgatcgt gaatgtaaag agcaacggca acgtggtcgt gaaccggcgc
      601 accctctccg agcccgacct cacgacgatg ttgcagggct tggtgaagct caatcccgac
      661 caggcggtcg tgattcgcgg cgacgaagcc ggcgcctaca agaacatcgt caacgtgctc
      721 aatatttgca gccaggccgg ggtcaccaac gtcgccttcg cgaccgcaaa atgaagccgc
      781 ggcgtcgcgc ggccacgctc cttgtcgcgg ccgcagtttt gcttttgcgg cgcgaagcac
      841 ctgcgcaaac gccgacgccc attcggcgcg ccatcccggt gcaggagcca cctgtcgcgc
      901 gcgccctccc cgtcgccaca ccgccgcctg ccaccgcgcc cacgatcatg cgcgcgctgc
      961 ccgtgccggt cgaaaccgaa ccggttgcgc cctcctttac cccaagccag tcgtccgctg
     1021 gcgggccgcc gccggaaagc gccgagccac cggagcaacg gcagctcaac tacgccaacg
     1081 cacttttcgg ccgcaagctc tacgatctcg ccgtcccgga atacgaaaaa tttctcggcc
     1141 tctacccgga ctcccccaac cgcgcgaccg cgctcttcta cctcggggaa gcgtatcgcg
     1201 cgctcggtcg caccccggcc gcgcgcacca gctttcaaag tgtcctgcag gattttcccg
     1261 agagcgacct ggccgggccc gcttcctatg ggctcgcgga gatcgccttc aacgagaaag
     1321 actatgcctc agcccttcct ctgttccatc gcgcggcggc gaaagtgaaa gcgtcggagc
     1381 tggcgctttc ggcgcgctat tttgagggcc gttgtctcga aaatctcgaa cgcagagatg
     1441 aagcgcgcga cgtctaccag caggtcatcg aagtaaacaa tccgaatccc tatcgtgatg
     1501 actcgcgctt tgccgtcgcg ctcatcttcc tcaccaatgg acgcaaagcc gacgccctga
     1561 aacaattcga agcgcttgct gaggagacga gcaagccgtc gctcaaggcg gaggcaacgg
     1621 tgcgagcggg cttggtcgca gtcgatctcg tgcaatcgga caaaggcaaa ccggacaagg
     1681 caatgattgc gaaagcgacc gccttgctgc aaaaaggccg cgccctgccg ggagcccgcc
     1741 gctggagcgg gatcgcgggc gtggggctgc tgcgtctcga gtatcaggcg ggtcagtacg
     1801 cgcaggccgt cgccgattat cagcgaagca aagagcaggt gccggaagag gtgcttcccg
     1861 agatgatgct gcttgccgcg aacagtcagc gccagctcag ccatttcaac gaagcgcagg
     1921 acctttatcg ccagatcatg caaaaatatc cgggccgcga ggaggcgaag gacgcgcgtt
     1981 accagcattt gatcggtctc tacaacgcca acgacccggc gttgcgcgat gagatcgacg
     2041 atttcatccg ggacaatccc gagagtgagc gcggtgacca ggccaagctg ctcaaagccg
     2101 aagcgctcta caaggagcat gatttcgccg ccgccgctct gctctataca gcgctgcgcg
     2161 attcgaagtt gtccgccaaa ctgcgcgctg aatccgcctt caaactcggc tggtgctttg
     2221 tgcaaacaaa ggaactggcc aacgtgatcg acgctttcgc ttattttctc caagctttcc
     2281 ccgataatcc gcaggtcccg tccgctttag cccagcgggc gctcgcctac caggaagaca
     2341 aacaatacga gagcgcccgg gccgatctcg accggctgct gaaaaactat cccgaggcac
     2401 gcgagcggga ggccgcactt cagcaaaaag ccctcatcct tggacaaacc gacgacgcgc
     2461 tcggaatgac cgcgacgttt caacagttgc tgaaggagtt tccgaagagc gcggccgcgg
     2521 cgcaggcgca ttattacatc ggcaaatcgg ccttcgaagc gaaggattac gagcgcgcga
     2581 tcgcagagat gaacaaggcg cgccagctga acccggagca ataccggacg ccggcagcgt
     2641 tgcgcatcat gtcgtcgtat ttctatctca agcagcgtgc cgcgctggcc aaagaggtga
     2701 accagttcta cgccgcttcg cctgacggac aggtgccggc ggaaattctc gaatggctcg
     2761 ggatcgattt ctacaatgcc cgggactacg ccgccgcggc caaatacctg accgcgctga
     2821 gcaaagccgc cattgtgagc agcgtgaaac cggatttctg gttttacctc ggcgacgcgg
     2881 aaatgaaatt gaatcaaccg gcggaggcgg aggttgcgct ccagaagttt ctcgaaaccg
     2941 tgagcgatcc ggcggcgaaa gcgaaagggc tccttgccct cggtgcagcc aagattgcgg
     3001 cgcacaagcc ggacgacgcg caaaaaatcg cggatgaaat catgaccttg caaccggaag
     3061 gccggaccaa cgcgcaggcg cgtctcctgg ccggagacgt cgagatggag cgccagcaat
     3121 ttgaagacgc gggtaaagca ttcatgagcg tcgcgttgct ttacgacgac ccggagatca
     3181 cgccccaggc gctcgccaag gccgcgaaag cctacgaaaa agcaggcaaa ctggacgagg
     3241 cacagcgggt caacgctcaa ctgcaccaaa agtatcccga ttacgccgga ggctagatta
     3301 gcctaacgat gacaaatcgt cgcggcggtt tccggggagc gcacgcgccc tcgcgtgctg
     3361 ggttcggcgc ctcgccgaac cctcgtcgcg ttcagggtgc gagtggcgcc tccccagcgt
     3421 gcaatcggcg aggcgccgat tgcggcacgc gagggcgcgt gcgctccccg gaaaccgcta
     3481 cgaccacggc agatgcaaca aagtttcaat cggcagcggc ccgagaaact tttgcggatc
     3541 atcaatctgc aggatcggtc gcgccaggca ggcggcgaga aaactaagat cgggcgtcgc
     3601 ggccagaccg gcctgcgcgg tcaattccaa ccgggcgagc tgcctaacga cgcgttcgat
     3661 attctcccgg caatttcttg ccgaggtaaa tcgcaccgtg caaacgccgg gtgcggtcgc
     3721 ctcgatctcc ggcgccaggg tcgccgcgtg atgcagcaaa atttcgccgg cgattttctc
     3781 ctgcgcgcgc gcccgcggtt tgaggagcag gcgcaaacag cgggccagcc cctggctcgg
     3841 cgtcatccct gcgctgacac cggcttccgc cgcgccgggc gtgagctgca tgatggtcgc
     3901 cttcgctgtc tcatcatcga ccagggcgag cggcttccgc tgcagctcgg gctcgtgccg
     3961 caaggccgcc tgcaattcaa aatccggcaa ataaagcgcc gcaaacatca gccggcccgg
     4021 gcgatttttg gatcgctcct ccatttgtta ggctgagcac gttgcaaccg aatcttcagt
     4081 tgcgcctgca agtcggccac ggccacatcg agctggagaa gcgtccagcg attttccaaa
     4141 accagtttcc attgcgcact gctgatcatg cttcgcgggg tgaaaaccag aaacgccgtc
     4201 ggcgccggct cgactaaacg ttgcaggcga taccagttgc tcgtgggaat ccggctcaat
     4261 tccgttagtg gattgagaac gagatcgagg atgacgagcg gaaaattccc gtcgcgcaaa
     4321 agcagatcgg ctgcctgcat cgcttcgctc gctttccgac agcgcaccca aaggagatgg
     4381 cgcaacgcgg cgttgccgag ggatggcggg gcgaacgaat cgcggccatc gatcagggcc
     4441 aggaaatagc gctccccgca ggcacgttgc agaagcgcgg cgatgatcga cgcgctgccg
     4501 gcactcggcg gcgcgctgct caattccgtg atcgcccctt tcgttaggcc accgcccagc
     4561 gcttcatcga aaaggcgcag gccggtgacc agccggtcga ccggcggcaa cgcttcctgc
     4621 gggaaacgtt ccgccaggag cttacgcaga tcgatgatcc tgctcgcggc catgccgaag
     4681 cctgccacga aatcgaaata tgttcaaaag aacagttaag ctaattctta acccggcgac
     4741 tgctttcgca ccagcgaggt catcactccc tggatgcgta actcccgcgc cggaaccaaa
     4801 tccgggaagc gtgggttctc cgctttcaaa tacggccgtc cgcgttccgt cacataacgt
     4861 ttcaaggttg tctcgccatc gatcaacgcc gccacgatgt cgccatttct tgcgctctgg
     4921 cgatcttcca ggatcacgat gtcgccatca agaatgtgcg caccgatcat ggaatcaccg
     4981 cgcacgcgca gggcgaaagt gcgcccgctc cgcgaggcgt taagcgattc gaggtcgagc
     5041 gccacatggc cctcgatatt ttgctcggcg agggtggcca tgccggcggg aatctccccg
     5101 tagatcggga tatcgaccac ccggctctgc ggcgcggcgg taatcaaggc gcgtgccttg
     5161 cgcgcatgac gctggagaaa ccctttccgc tcgagcgcgg caatgtattg catgaccgag
     5221 gtctgactcg cgaagccgaa gtgttcctga atctcccgcg tgctcggggt cagccctttg
     5281 gtgcgctgtt ccgcctggat gaattgcagg acgttcttct gcccgggcgt gagcatatga
     5341 acatgttggc gaaaacctc
//
LOCUS       DATMNS010000195         5090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1702881, whole genome shotgun sequence.
ACCESSION   DATMNS010000195 DATMNS010000000
VERSION     DATMNS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5090
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702881"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VNW28_06650"
     CDS             <1..375
                     /locus_tag="VNW28_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09644.1"
                     /translation="SAIEEKLIQVKMKSSEGNLVYPNELNEEFYTFTQSIEADTAPTQ
                     PQEEVFKQLAGQLEEQLKAWTQITTDDVPKVNALVKQADLPALTVASAAPPNESPTPK
                     PTPTAPTESPAPTASPTPSAKP"
     gene            447..1352
                     /locus_tag="VNW28_06655"
     CDS             447..1352
                     /locus_tag="VNW28_06655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HXA09645.1"
                     /translation="MSHSHQPQIDFGRAFALGVLLNTGFIVAEVVFGLLSHSLSLLAD
                     AGHNLGDVLGLLMAWAATGLERRSPTQRFTYGLRRTSVLAALCNAILLLVSVGAIALE
                     AVRRIREPAPVAGLTMIWVATAGVVVNGLTAWMFAGGRKNDLNIRGAFLHMAADAAVS
                     AGVVVAGFVILRTGWDLLDPISSLVIVVIILAGTWGLLRDAINLALDAVPRGIDPGAV
                     KVFLEQLPGVAAIHHLHVWGLSTTETACTAHVVKREPQLDDQLLKRIAHELHDRFGIE
                     HTTIQFERCDGPDCPTEHETHAAHD"
     gene            1327..2061
                     /locus_tag="VNW28_06660"
     CDS             1327..2061
                     /locus_tag="VNW28_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXA09646.1"
                     /translation="MKRTLLMTDVMTKAVLLAAGRGTRMRDLTNELPKPMIEVQGKPI
                     LQHIIEGLRAAGVQQFLIIVGYRAEVVRRAFGDGARLGVEINYATQVVQNGTGKVVEL
                     ARDFAGEDAFVLSYGDIIVDPSNYPRLVALEAGTEALVSIRPNEDVSQGGAVFVNERF
                     ELIDLREKPRPGEPTSPWYNAGIYAFRPSIFDFTARLQPSPRGEFELTDAVRDLAHSG
                     RKVQAIELHGAWADVRDPEILARLNR"
     gene            complement(2058..2885)
                     /gene="pssA"
                     /locus_tag="VNW28_06665"
     CDS             complement(2058..2885)
                     /gene="pssA"
                     /locus_tag="VNW28_06665"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HXA09647.1"
                     /translation="MRDPQNPKIYLLPNLMTAGNLFCGFAAVLRIYDGAQRIDLLEAA
                     SDFHDAIWFILAACIFDLLDGRLARLGGHESSFGREFDSLADIVSFGLAPALLVYQIV
                     LGEFQNLGWIIAFVYLACGALRLARFNCVAEAGSPTSPDKNFTGFPIPAAAGVIASLT
                     LFMLWLDAGQRTIGRWKYALPALMLFLSFMMFSKFRYPSFKAVNWRTTQSIPRFLFLI
                     ALLALTVKFYNWMAAVIFLTYLLYGFLRPFLSPKRRRVIEEEIGEEPPETETIEEIP"
     gene            complement(2888..3517)
                     /locus_tag="VNW28_06670"
     CDS             complement(2888..3517)
                     /locus_tag="VNW28_06670"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003685.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HXA09648.1"
                     /translation="MRLQTLFEARWIFAVLLVLTLASLWLVPWLALLFLLLISYTFVF
                     FRDPDRTVPADPHVVVAAADGVVVEIAELAENEVIKETMRRVAIFLSVFDVHTNRAPI
                     DGRIIYREHRAGLCLDARNPECSQKNEAMTWAFENPRATLVVRQITGAIARRIVGWSQ
                     VGDRLQKGERFGMIRFGSRTEVYLPLSAAVLVKVGDRVAGGASPIARLA"
     gene            3751..4383
                     /gene="upp"
                     /locus_tag="VNW28_06675"
     CDS             3751..4383
                     /gene="upp"
                     /locus_tag="VNW28_06675"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HXA09649.1"
                     /translation="MRADAVRTLDHPLAAAQLSVLRAKATPPNEFRTALQQLSVLLLI
                     EAARSWETRTLAQESPLKAFTAFVLARPVTLVPILRAGLGMQEGMMPLVPTASTGHVG
                     LYRDPKTLRPVQYYSRLPAHLPEAEVLVIDPMLATGQSAVAAVTLVKAAGARRLQFVC
                     VLGCPPGVAELQRAHPDVPIIAAAIDPELNDAGYIVPGLGDAGDRYFGTL"
     gene            4504..>5090
                     /locus_tag="VNW28_06680"
     CDS             4504..>5090
                     /locus_tag="VNW28_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09650.1"
                     /translation="MDPDEPRIEIFAPFGEAFELTKKILFQPFDIGKWFVIGFAAWLA
                     TFFNGAGFNYRTWNRNNDWNWSSKAHHHGAPFSLHDMPPWVIPVVVIGVLLVLALITV
                     LLWLNARGRFMFTDCIVRNRAAIAEPWREFQTEANRYFVFSIVITLASLVVFGSLGLI
                     IFIDWYWSNPVVPIALLILIGVAVVLAAVIVGLVMR"
BASE COUNT          992 a         1629 c         1538 g          931 t
ORIGIN      
        1 tccgccatcg aggagaaact catccaggtg aaaatgaaaa gttcggaagg gaatctcgtt
       61 tatccgaatg aactgaacga agaattctat actttcaccc aatccattga agccgacacg
      121 gcgcccaccc aaccgcagga ggaagtcttc aagcagctcg ctggacaact cgaagagcag
      181 ttgaaagcgt ggacgcagat cacgaccgac gatgtgccga aggtcaacgc cctggtcaag
      241 caagccgatc tgccggcgtt gacggtcgca tccgcggccc cgcccaatga atcaccgacc
      301 ccgaagccaa ctccaaccgc gcctacggaa tcaccggcgc ccaccgcatc gccgacaccc
      361 tcggccaagc catgaaggga gaggtgcgga agcacgtccc tccggaggaa gcacccgttt
      421 caggtttcga atcgtttcgt taggcgatga gccattcgca tcagccgcaa atcgatttcg
      481 ggcgcgcctt cgccctcggc gtgctcctca acaccggctt catcgtcgcg gaggttgtct
      541 tcggcttgct cagccattcg ctctcactcc tggccgatgc cggccataat ctcggcgatg
      601 tcctcgggct cctgatggcc tgggcggcga ccggcctcga acgccgcagc ccgacccagc
      661 gcttcaccta cggtctccgc cgcacctccg tgctcgcggc gctttgcaac gcgatcctcc
      721 tcctcgtcag tgtcggcgcg attgccctcg aagcggtccg ccgtattcgc gagcccgcgc
      781 cggtcgccgg cctaacgatg atctgggtcg cgacggcggg cgtggtggtc aacggtctca
      841 ccgcctggat gtttgccggc ggacggaaaa acgatctcaa cattcgcggg gccttccttc
      901 acatggcggc cgacgcggct gtttcggcgg gcgtggtggt ggccggattc gtcatcctgc
      961 gcaccggctg ggacttgctc gacccgatca gcagcctcgt catcgtcgtg atcatcctcg
     1021 ccggaacgtg gggcctgcta cgcgatgcga tcaacctcgc gctcgatgcc gtgccccgcg
     1081 ggatcgatcc cggcgcggtc aaggtttttc tcgaacaact gcccggcgtc gccgccattc
     1141 accatctcca tgtctggggt ctgagcacga ccgagaccgc ctgcaccgcc catgtcgtga
     1201 agcgcgaacc gcaactcgac gaccagttgc tcaagcggat cgcgcacgaa ttgcacgacc
     1261 gtttcgggat cgagcacacc accattcagt tcgagcgctg cgacggaccg gattgcccga
     1321 ccgaacatga aacgcacgct gctcatgact gacgtcatga ccaaggcggt cctcctggcc
     1381 gccggtcgag ggacgcggat gcgcgacctg actaacgagt tgcccaaacc catgatcgag
     1441 gtgcagggca aaccgatcct ccagcacatc atcgagggtc tgcgcgccgc cggcgtgcag
     1501 cagttcctca tcatcgttgg ttatcgggcc gaggtcgtca ggcgagcttt tggcgacggg
     1561 gcgcgactgg gcgttgagat caactatgcg acgcaggtgg tgcagaacgg caccggcaag
     1621 gtcgtcgagt tggcgcgtga tttcgccggc gaggatgcct tcgtcctgag ctacggcgat
     1681 atcatcgtcg acccgtcaaa ttatccgcgc ctcgtcgcgc ttgaagccgg gaccgaggcg
     1741 ctggtcagca tccggcctaa cgaggatgta agccagggcg gcgccgtttt tgtgaacgaa
     1801 cgcttcgagc tgatcgattt gcgggaaaaa ccgcgacccg gcgagccgac gagcccgtgg
     1861 tacaacgccg ggatctacgc ttttcgcccg agcattttcg atttcaccgc ccggctgcag
     1921 ccttcgcccc gcggcgaatt cgagctgacg gacgcggtcc gcgacctcgc ccattccggc
     1981 cggaaagtgc aggcgatcga gttgcacggc gcctgggccg acgtgcgcga cccggagatt
     2041 ctcgcccgcc tgaatcgtta ggggatttcc tcgattgtct cggtctccgg cggttcttcg
     2101 ccgatttcct cttcgatcac gcgacgccgt ttgggcgaga ggaaaggccg cagaaatccg
     2161 taaagcaggt aggtgagaaa aatcaccgcc gccatccagt tataaaactt cacggtcaag
     2221 gcgagaagcg cgatcaggaa aaggaagcgc ggaatcgatt gcgtggtgcg ccagttgacc
     2281 gccttaaagc tcgggtagcg gaatttgctg aacatcatga aggagagaaa aagcatcagg
     2341 gccggaaggg cgtacttcca gcgaccgatg gtgcgctggc cggcgtcgag ccagagcatg
     2401 aacaacgtaa gcgacgcgat cacgcccgcc gccgccggga tcgggaaccc ggtaaaattc
     2461 ttgtccgggc tggtgggcga gccggcctcg gcgacgcaat taaaccgcgc caggcggagc
     2521 gcgccgcacg ccaggtaaac gaacgcgatg atccagccga ggttctggaa ttcgccgagc
     2581 acgatctggt aaacgagcag ggccggcgcc agcccgaagg aaacgatatc ggccagcgaa
     2641 tcgaactccc ggccaaagga actctcgtga ccgccgagcc gcgcgagccg gccatcgagg
     2701 agatcgaaaa tacaggccgc gagaatgaac cagatcgcat cgtgaaaatc ggaggccgct
     2761 tcgaggagat cgatcctctg ggcgccatca taaatccgga gcacggccgc aaagccgcag
     2821 aagaggttgc ccgccgtcat caaattcggc aggagataaa tcttcggatt ttgcgggtcg
     2881 cgcatgatca ggccaaccgt gcaatcgggc tcgctccgcc ggcgacgcgg tcgcctacct
     2941 tgacgagcac cgccgcgctc agcggcaggt agacttcagt ccgtgaacca aatcgaatca
     3001 tcccgaatcg ctcgcccttt tgcaggcgat cgccgacctg cgaccagccg acgatccggc
     3061 gcgcaatcgc gccggtgatt tgcctcacga ccagggtcgc gcgcggattc tcaaacgccc
     3121 aggtcatggc ttcgtttttc tgcgagcact cggggttgcg cgcgtcgagg caaaggccgg
     3181 cccggtgttc gcggtaaatg atccggccat cgatcggcgc gcgattggtg tggacgtcaa
     3241 aaacagaaag aaaaatcgcc acgcggcgca tcgtttcttt gatgacttcg ttttcggcaa
     3301 gttcggcaat ctccacgacc acgccatcgg ctgcggcgac gacgacgtgc ggatccgccg
     3361 gcacggtccg gtcagggtcg cgaaaaaaga cgaaggtgta gctgatgagc aggaggaaaa
     3421 gcaacgccag ccagggcacg agccagaggc tcgccagcgt gaggacgagg agcacggcga
     3481 agatccagcg tgcttcaaaa agcgtttgca agcgcatgag cggggagagt ttcgagttcg
     3541 tcggtaggcg ggtcaagtgg atcgcacctc tagccgcctc cctgtgggag gcgcgggtct
     3601 cagcgtgaac tggacgatga ctcgggggcg cgtcagctgc ctcgcattgc cccctccccc
     3661 gcgcttcgca cagcgaagcg tctacaagcg ccaaccgcgt cgtgcacgtt ttcaggtgac
     3721 caaaggattc gcccgcgcca tcatccgagc atgcgcgcgg acgcagtacg aactcttgat
     3781 catcccctcg ccgccgcgca gctctcggtc ctgcgcgcca aggcgacgcc gcctaacgaa
     3841 ttccggaccg cgctgcagca actctcggta ctcctgctca tcgaagcggc ccggagttgg
     3901 gaaacgcgaa ctctcgccca ggaaagcccg ctcaaggcat ttaccgcttt cgtcctggca
     3961 cgaccggtca ctctcgtgcc gatcctgcgc gcgggtctcg ggatgcagga gggcatgatg
     4021 ccgctggttc cgacagccag caccggccac gtcggacttt accgcgaccc gaagacgctg
     4081 cgcccggtgc aatactattc ccggctgccc gcccatctcc cagaggcgga ggtgttggtg
     4141 atcgatccaa tgctggccac cggtcaaagc gcggtcgcag ccgtcacgct ggtgaaggcc
     4201 gccggagcga ggcggctgca attcgtgtgc gtcctgggtt gtccgccagg tgtggcggag
     4261 ttgcagcgcg cgcatcccga cgtgccgatt atcgcggccg cgatcgatcc ggaattgaac
     4321 gacgccggct acatcgtgcc cggactgggc gatgccggcg atcgctattt cggcacgctg
     4381 tgatttttct cgtgctcatg ctcgtaatcg aaatccgcca tcgagcacga gcaagagcgc
     4441 gatgacgagt gagtcgcgcg ttccgagctt gcctcaaccc cccgaacctg attggaattt
     4501 cccatggacc cagacgaacc gcggatcgaa atcttcgccc cattcggtga agcatttgaa
     4561 ttaacgaaga aaattctttt tcagccgttc gacatcggaa agtggttcgt catcggcttc
     4621 gccgcctggc tcgcgacctt cttcaacggc gccggcttca actatcggac ttggaatcgg
     4681 aacaacgatt ggaattggag ttcgaaagcg catcatcacg gcgccccctt ttccctccat
     4741 gacatgccgc cgtgggtcat tccggtggtc gtgatcggcg tcctcctcgt gctcgcgctt
     4801 atcaccgtcc tgctctggct caacgcgcgc ggtcgtttca tgttcaccga ctgcatcgtc
     4861 cgcaaccgcg ccgcaatcgc cgagccctgg cgcgaattcc agacggaagc caatcgctac
     4921 ttcgttttct cgatcgtcat cacactcgcc agtctggtcg tcttcggtag cctcggcttg
     4981 atcattttca tcgactggta ctggagcaac ccggtggtgc cgatcgcgct tctcatcctg
     5041 atcggagtcg ccgtcgtcct tgcagcagta atcgtcggcc tggtcatgcg
//
LOCUS       DATMNS010000196         5782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3359696, whole genome shotgun sequence.
ACCESSION   DATMNS010000196 DATMNS010000000
VERSION     DATMNS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3359696"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..274)
                     /locus_tag="VNW28_06685"
     CDS             complement(<1..274)
                     /locus_tag="VNW28_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HXA09651.1"
                     /translation="MADPMDETFMRAALAAARRGVGHASPNPAVGAVLVQGGRITATG
                     YHRASGLPHAEIECLATAPGCSLRSATLYVTLEPCSTTGRTPPCTKA"
     gene            643..1032
                     /locus_tag="VNW28_06690"
     CDS             643..1032
                     /locus_tag="VNW28_06690"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXA09652.1"
                     /translation="MNSLFDLPATGNFARQAQLFSGWAPALDLHQNNDNVVAIVELPG
                     MRKEDIDISLHDGMLTIAGERQSSTSDGENAERTERFSGKFRRSITLPTRVDAGKVTA
                     SYKDGILTVTLPKVEEAKPKKVEVTIA"
     gene            1084..1464
                     /locus_tag="VNW28_06695"
     CDS             1084..1464
                     /locus_tag="VNW28_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXA09653.1"
                     /translation="MNNTSVRENNGRAANRSESEKFVAPTASVSETADGYTLELEMPG
                     VNKEGLEISIEKNELSIVGRRSNPAINGTLVHRESRPHNYRRSFEIDPSIDSGKIVAR
                     MNQGVVTLTLPKAEAVKPRKITVS"
     gene            1602..2642
                     /gene="recA"
                     /locus_tag="VNW28_06700"
     CDS             1602..2642
                     /gene="recA"
                     /locus_tag="VNW28_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXA09654.1"
                     /translation="MATKTEEKPAAEDKLIAARNKNLDLAIQQIAKDYGDGAIMRLGD
                     EKIVDIDVIPTGNILIDRALGVGGFPRGRVVEVYGPESSGKTTLTLTVIAQAQKRGGL
                     AAFIDVEHALDPQYARRLGVDLDDLLVSQPSSGEEALRIVETLVRSNALDVIVLDSVA
                     ALVTKQELEGEIGDSTVGAQARLMSAAMRKLTSLISKARTCCIFTNQIREKIGVMFGN
                     PETTPGGKALKFYASVRIDIRRIGAIKQTDGVVTGNRTRVKVVKNKVAPPFTEAEFDI
                     MYNEGISSTGALLDLALEKQIVEKRGSWLSYKGAQLAQGRDAAKEVLKNDPALYEEIE
                     TAVKAKLDEEKK"
     gene            2827..5679
                     /gene="alaS"
                     /locus_tag="VNW28_06705"
     CDS             2827..5679
                     /gene="alaS"
                     /locus_tag="VNW28_06705"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXA09655.1"
                     /translation="MTSAEIRQSFLDFFREKKHTVVPSSSLLPDAPNLLFTNAGMNQF
                     VPIFLGQQRPSFDPPRAADTQKCIRAGGKHNDLDDVGLDTYHHTFFEMLGNWSFGDYF
                     KQEAIEWAWELVVRRWKFPPERLYATVYRPGLSEPADFDQEAHDHWHRLFTATGLDAA
                     VHVVNGNKKDNFWMMGDTGPCGPCSELHVDLTPLGDTRGALVNAGDPRCIEIWNLVFI
                     QFNANPDGTFSPLPARHVDTGMGFERVTAIIQGTKNFSDFTGTISNYETDIFRPIFDA
                     IEKFSGKTYTSTLPKSGAAAESEQEKIDVAFRVVADHIRTLSFAIADGIQPSNEGRGY
                     VLRRILRRAVRYGRSLGRHEPFFYRLVEVLALTMGDVFPELTENQAHIEATLKREEES
                     FNRTLDKGIALFEAEVARLQPPGSARDSRAVSGDPPETSPASETRYAKRRLPHFERPW
                     GKYAITFSTEGRRPLTAAERDIVLDSILFPAKRNHFELYVACVMPDHVHLLLEPQVES
                     QDGNGNAVFFPLTELLQNIKSVSAHRINKARGAVGPVWEKESFDRLIRSEADLQEKYL
                     YICKNPWESRIAQPNEDYRWLWTPESARAPGESPDAARESRALPGAHNATQISGAFAF
                     RLYDEQGFPLDLTELMARERGLTVDTEGFEKLMEEQRARGRSAQKKEVIAVSQGIPPW
                     TTTFVGYNDLRSEGGHYMLRGDQLFVEITPFYAEMGGQVGDTGHVQTLRGSTVEITNT
                     LRAPTGHYILRVGDPSAFEEPDDVPFRYIVNAPRRAAIERHHTVTHLLHWALHEVVSR
                     DATQKGSYVGPEKLTFDFSSAALTEPQVREVEKLVNEKIAENAPVSWTEIPYPEAKRR
                     ANIMQFFGDKYGDMVRVVQIGGVPNELNGYSMELCGGTHVRATGDIGSFRILSEGAIA
                     AGIRRIEAVAGHAVGEWAQAEAARQEEIWARGLARV"
BASE COUNT         1291 a         1811 c         1661 g         1019 t
ORIGIN      
        1 tagccttggt gcacggagga gtgcgtccgg tcgtcgagca gggctcaagc gtgacgtaga
       61 gggtggccga ccgcagcgag cacccggggg ccgtcgcaag gcactcgatc tctgcgtgcg
      121 gcaaaccgct cgcccgatga tagccggtgg ccgtgatgcg acctccctgc acgagcactg
      181 cgcccaccgc cgggttgggg ctggcgtgcc cgactccgcg ccgcgccgcg gcaagcgccg
      241 cccgcatgaa agtctcgtcc atgggatctg ccatcgccga accctaagca gctcgatcgc
      301 agatcgcaat ggattccgca tttcattcgc tgtttggaac aaccgtgaga agtaaccttc
      361 tggcacaacc accgatacca cagcggctat ctaattgcgc cgttatggcg cgattaacgt
      421 cggatctcgt gcgaatatat taatcgataa tatattcata tcaaaatact taactcgata
      481 aatatcaaga actcaatgcg tggcatccga tgagctagct aaacaaccac tgaacaaaca
      541 gtttagcaga aaggaaaaat cattatgagc ctaattcgtt accaatctcc cgaaatttca
      601 acctggcccg cgtttgctcg atgggctgat ctccgagacg agatgaacag cctcttcgac
      661 ctgccagcga cgggcaattt tgcccggcaa gcacagctct tcagcggctg ggcgccggct
      721 ctcgacctgc accagaacaa cgacaacgtt gtcgcgatcg tcgagctgcc cgggatgcgc
      781 aaggaggaca tcgatatctc cctgcatgac ggcatgctga cgatcgccgg ggaacgccag
      841 agttccacga gcgacgggga aaatgccgag cgcaccgagc gcttcagcgg caaattccgc
      901 cgcagcatca ccctgccaac gcgcgtcgac gccggcaaag tcaccgcctc ctacaaggat
      961 ggcatcctga ccgtcacgct cccgaaagtc gaggaggcaa agcctaaaaa agtggaagtc
     1021 acgatcgcat aatcacggcc ggcgaaccga gttcgccggc cccaattcag gaaaggaaat
     1081 tctatgaaca acacctcagt gcgcgaaaat aacggaaggg cggcgaaccg ctccgagagc
     1141 gagaagttcg tggcgcctac cgccagcgtg agcgagaccg ccgatggcta cacgctggaa
     1201 ctcgaaatgc cgggcgtgaa caaggagggc ctggaaatct cgatcgagaa aaacgaactc
     1261 tcgatcgtcg gtcggcgctc caacccggcc attaatggaa ccctcgtcca tcgcgaatcc
     1321 cgcccgcaca attatcgccg ctcgtttgag atcgatccct cgatcgatag cggaaaaatt
     1381 gtcgcccgga tgaatcaggg cgtcgtgact ctgacgctcc cgaaggccga agcggtgaaa
     1441 ccgcgcaaga tcaccgtctc gtaattccaa ttgggttagg taaacagaag ccgcgggtcg
     1501 cgaggtccgc ggctttttcc gttcccaact aatctttttc gccgcccgcg cgcttttcta
     1561 cttggcgcgc ccgtcgccgc gtcgtaccta gagagcccct tatggccacc aaaaccgaag
     1621 aaaaacccgc cgccgaagac aaactgatcg ccgcgcgcaa caagaacctc gacctcgcca
     1681 tccagcagat cgccaaggat tatggcgacg gcgcgatcat gcggctcggc gatgagaaaa
     1741 tcgtcgacat cgatgtcatc ccgaccggca acatcctgat cgaccgcgcg ctcggtgtgg
     1801 gcggatttcc ccgcggacgc gtggtggaag tctacggacc agaatcctcc ggcaaaacca
     1861 ccctgacgct gacggtgatc gcacaagcgc agaagcgcgg agggctcgct gctttcatcg
     1921 acgtcgagca tgcgctcgat ccgcaatacg cccgccgcct cggcgtcgac ctggatgatt
     1981 tgctcgtctc gcaaccgagt tcgggtgagg aagcgctgcg aattgtggag acattggtgc
     2041 gctcgaatgc cctcgatgtc atcgtgctgg actcggtcgc ggccctcgtc acaaaacagg
     2101 aactcgaagg agaaatcggc gactccacgg tcggcgcgca ggcgcggctg atgagcgccg
     2161 cgatgcgcaa acttacctcg ctcatctcaa aagcgcgcac ctgctgcatc ttcaccaacc
     2221 agattcgcga aaagatcggc gtcatgttcg gcaaccccga gacgacgccg ggcggaaagg
     2281 cgctcaagtt ctacgccagc gtccgcatcg atatccggcg catcggggcg atcaagcaga
     2341 ccgacggcgt cgtgaccggc aaccgcaccc gggtgaaagt ggtgaaaaac aaagtcgcgc
     2401 cgcccttcac cgaagcggag tttgacatca tgtacaacga gggaatttcc tcgaccggcg
     2461 cgctgctcga tctcgcgctc gagaaacaaa tcgtggagaa gcgcggctca tggttgagct
     2521 ataaaggcgc gcaactcgcc cagggtcgtg acgccgcgaa agaggtcttg aagaacgatc
     2581 ccgcgctcta cgaagaaatc gagacggcgg ttaaagcgaa gctcgacgaa gaaaaaaagt
     2641 agccgcaaaa acgaaccgtc gtgcatgatg ctcccgcctg ggtagcgcac gcgttccgcg
     2701 tgttggtcgc ggcattctgc cgcggcgaac ttttcgtaag gcacggggca cacagaaggc
     2761 gtgcgctcgc cgggcgatcg atcacacgcc gatgaaaaat tcctcattca cgctaaactc
     2821 cacccgatga cttccgccga gattcgccaa tcatttctcg atttctttcg cgagaaaaag
     2881 cacaccgttg tgccctcatc ctcccttctc cccgacgcgc ccaatctcct tttcaccaac
     2941 gcgggcatga atcagttcgt gccgattttc ctcgggcaac aacggccgag cttcgatccg
     3001 ccgcgcgccg ccgacaccca gaaatgcatc cgggccgggg gcaagcataa cgacctcgac
     3061 gacgtcgggc tcgacacgta tcaccacacc tttttcgaaa tgctcggcaa ctggagcttc
     3121 ggcgattact tcaagcagga agcgatcgaa tgggcgtggg agctggtcgt taggcgctgg
     3181 aaatttccgc ccgagcggct ttatgccacc gtctaccgcc ccggcctgag cgagcccgcc
     3241 gatttcgacc aggaggcgca cgaccactgg caccggctct tcaccgcgac cggcctcgac
     3301 gccgccgtcc acgtcgtgaa cgggaacaag aaagacaatt tctggatgat gggcgacacc
     3361 ggcccgtgcg gaccgtgcag cgagttgcac gtcgatctta ctccgctagg cgacacccgc
     3421 ggtgcgctcg tcaacgccgg cgacccgcgc tgcatcgaga tttggaacct ggttttcatc
     3481 cagttcaacg ccaatcctga tggcacgttt tctcccctgc ccgcccgtca tgtcgacacc
     3541 ggaatgggct tcgagcgcgt cactgcgatc atccagggca cgaaaaattt ctccgatttc
     3601 acgggcacga tctcgaacta cgagaccgac atctttcggc ccattttcga cgcgatcgaa
     3661 aaattcagcg gcaagactta cacctcgacg ctgccaaaat ctggcgctgc cgcggaaagc
     3721 gagcaggaaa aaattgacgt cgccttccgc gtcgtcgccg accacatccg caccctcagc
     3781 ttcgcgatcg ccgacggcat ccaaccgagc aatgaggggc gcggttacgt tttgcggcgc
     3841 atcctgcgcc gcgccgtccg ctacggacgt tcgttaggcc ggcacgagcc ttttttctac
     3901 cggctggtcg aagtcctcgc cctaacgatg ggcgatgttt tccccgagct gaccgaaaac
     3961 caggcgcata tcgaagcaac gctgaaacgc gaagaggaat ccttcaaccg cacgctcgac
     4021 aaaggaatcg ccctcttcga agccgaggtc gcgcgccttc agcctccggg gagcgcgcgc
     4081 gactcgcgtg ccgtttccgg cgacccgccg gaaacgtccc ccgcatctga aacgcgctat
     4141 gccaaacgcc gcctccccca ctttgagcgg ccgtggggca aatacgcgat caccttcagc
     4201 accgaaggcc gtcgtcccct gaccgccgcg gaacgcgata tcgtcctcga ctccatcctt
     4261 tttccggcga agcgaaatca cttcgaactt tatgtcgctt gcgtcatgcc cgatcacgtc
     4321 catctcctcc tcgagccgca agtcgagtcg caggacggga acggtaatgc cgtctttttt
     4381 ccgctcaccg aactcctgca aaacatcaaa tccgtctccg cccatcgaat caacaaagcg
     4441 cgcggggcgg ttggacccgt gtgggaaaag gaatcgttcg atcgtttgat ccggtctgaa
     4501 gccgatctcc aggaaaaata cctctacatt tgtaagaatc cctgggaatc gaggatcgca
     4561 cagccgaatg aggactatcg gtggctctgg acacctgaga gcgcgcgagc gcccggcgag
     4621 tcgccggacg cggcacgcga gtcgcgtgcg ctcccaggag cgcacaacgc gacgcagatc
     4681 tctggcgcgt tcgcttttcg gctttatgac gaacaggggt ttccgctcga tctgacggaa
     4741 ttaatggcgc gcgaacgcgg gctcacggtt gatacggaag gtttcgagaa attgatggag
     4801 gaacagcggg cccgcggccg gtcggcgcag aagaaggagg tgatcgccgt ttcgcaaggc
     4861 attcctccct ggacaacgac attcgtgggt tacaacgatc tccgtagcga gggcgggcac
     4921 tacatgcttc gaggcgatca gctgttcgtt gagatcacac ctttttacgc tgaaatgggc
     4981 gggcaggtcg gagacaccgg ccatgttcaa acgctcaggg gaagcacggt tgaaatcacc
     5041 aacacattac gcgcaccgac cggacattac attcttagag ttggagaccc gtcggccttc
     5101 gaagaacccg atgacgttcc cttccgttat atagtcaatg cgccgcgccg cgcggcgatc
     5161 gagcggcacc acacggtgac ccacctcctt cactgggcgc tgcacgaagt cgtgagtcgc
     5221 gacgcgacgc agaaaggcag ctacgtcgga cccgagaaac tgaccttcga tttctcgagc
     5281 gccgccctga cggaacctca ggtgcgcgag gtggagaagc tggtgaacga aaagatcgcg
     5341 gagaacgcgc ccgtttcgtg gacagaaatc ccctacccgg aagcgaagcg gcgggccaac
     5401 atcatgcagt tcttcggcga caaatacggc gacatggtgc gcgtcgtgca gatcggcggc
     5461 gtgcctaacg agttgaacgg ttactccatg gagctctgtg gtggcaccca tgtccgcgcg
     5521 accggcgata tcggatcgtt ccgcatcctg agcgaaggcg cgatcgcggc cgggattcga
     5581 cggattgagg cggtcgcggg ccacgcggtt ggcgaatggg cgcaggccga agccgcgcgc
     5641 caggaagaaa tctgggctcg cggccttgcc cgcgtttgaa acgacgaacg cggcggcgat
     5701 gacggccagc gtcgacaccc gcgccgcgca gcttgaaaaa atcgaaaacg aggtgcgcga
     5761 ttgggaaaag gcgaacgcca gg
//
LOCUS       DATMNS010000197         6345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1708025, whole genome shotgun sequence.
ACCESSION   DATMNS010000197 DATMNS010000000
VERSION     DATMNS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1708025"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VNW28_06710"
     CDS             <1..199
                     /locus_tag="VNW28_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HXA09656.1"
                     /translation="DSTIITNPSDSLDHAPSVQNVGSHMGLDATRKLPGEGYHRGWPE
                     LVRMNEETRKLVDALRRSRGR"
     gene            complement(270..671)
                     /locus_tag="VNW28_06715"
     CDS             complement(270..671)
                     /locus_tag="VNW28_06715"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HXA09657.1"
                     /translation="MKKYFLPAVSAAILLATGLSSCETPAGQGAGFGAFSGAATGALI
                     GAAASGTGEGAAIGAGAGALAGALLGAAVAEDQARYYHAPPDGYPYARPTATAGYVYS
                     PYAPHNVIDVRGVPQGALVRDPSTNGIFRRP"
     gene            complement(723..1586)
                     /locus_tag="VNW28_06720"
     CDS             complement(723..1586)
                     /locus_tag="VNW28_06720"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HXA09658.1"
                     /translation="MKAGSVTDAMPTDSSSAEQITRASKSNLAFAFVSLGAERRRDIT
                     TFYAFCRLIDDIADATDLATEEKARRLTVWRKALHGPVPGESPVAPAVRGLLGQYAIT
                     PAMLEEIIAGVEMDLTISHYETFAALREYCYRVASAVGLVSIEIFGYRNPACRDYAIE
                     LGLALQVTNILRDVRKDLASGRVYLPREDMARFAYSPEDLAARIHDDRFVRLMQFEAD
                     RAHGYFQNAATLLPREDRRSMVSAQIMASIYRALLRQMEEDRFRVFARDYGLSKFAKL
                     LHVTRQLLKLP"
     gene            complement(1564..2742)
                     /locus_tag="VNW28_06725"
     CDS             complement(1564..2742)
                     /locus_tag="VNW28_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09659.1"
                     /translation="MFSAVPRVYPLLGMFGGYLLVMRTNPVRVALRDGFRCTLRFKRL
                     WLLFALFAFAYSTFQFAVFTPLQSTDDLRLDQLAFWDSWHWPLFSQVWRESLLHMVEA
                     VAGIFDAAATTFPLAFIAALLLIVNWRGLHRSLVRALRKQFGLGGWLIYFGVLLGALA
                     SLLKPLIYWQLPDRARFLSPAHLLQTSAAVDTVAFVFEYLCAVYLQVYLITVCLAWIK
                     GLHFREGALLRFAMRRFSFVLRWLGLLVLASTLLLRLPLLIAYFRDVPGVLDYLPAGR
                     YAMSALIVSFAAMQVSLVLHNETLREAFQAHWQFVRRNAFRFGWFLLICALHYWFLTF
                     SDAVIRTAATERPLALILWPLCYDLARALVTGWLLASWVCFFRQCEIGRANRESWIRY
                     "
     gene            complement(2764..3855)
                     /gene="lepB"
                     /locus_tag="VNW28_06730"
     CDS             complement(2764..3855)
                     /gene="lepB"
                     /locus_tag="VNW28_06730"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXA09660.1"
                     /translation="MFQPRYIKHCHLLVRHAEKLIRYRRDRLSPATAAELQAQIEKVR
                     AAMKARDNRASHEESERLDALLTMHTPPHKDAVWRENVEVILVAIVVAIGIRSYFLQP
                     FKIPTGSMQPTLNGIIGHPTNEPAPNFLKQIVDFLVLGRNYINVVSEEDNTVENVTSK
                     KFLFFFTLSGIECDHQSFTVYAPPEILRQDFQVYPGNRYRRGQVIARGAIDTGDQVFV
                     DKCSYNFVPPSRGDVFVFRTNHILGIREDPEAGAPFYIKRLTGTPGDELRIDSPKLYI
                     NGEPATRFGIRRVMAAKDPYRGYAPGQAFLGAPDRSFVIPPHSYFAMGDNSYNSYDSR
                     YWGPVPEENLVGRGLFVYWPFNRHWGFIR"
     gene            complement(3962..5761)
                     /gene="lepA"
                     /locus_tag="VNW28_06735"
     CDS             complement(3962..5761)
                     /gene="lepA"
                     /locus_tag="VNW28_06735"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXA09661.1"
                     /translation="MSIELTRNFCIIAHIDHGKTTLSDRLLERTGTIHDRDRQDQLLD
                     SMDLERERGITIKAHPVAMRYTAKDGQEYRLNLLDTPGHVDFAYEVSRSLAACEGALL
                     IVDAAQGVEAQTVANVHLAHKQGLTIVPVINKIDLPNADLPSVHRQLEEILAIPAEEA
                     IDCSAKMGIGIEEILEAVVHRIPPPPRPGDEVLRALVFDSVFDIYRGVVAYVRVFSGK
                     VEANQQIKVMSTDTRYEVKEVGVFTPKMFAQATLNAGDVGYVIANIKTTSELKIGDTM
                     TDLRHPAEKPLPGFQEIHPMVFSGIYPINTADFEHLKTAISKLRMNDSAFVYQPESSV
                     ALGFGYRCGFLGLLHMEIIQERLRREYDMDIIATSPSVIYEVLTTRGEIVLVDNPAHL
                     PDPSVIEEIREPIVRAYILCPNECIGDILQLILEKRGTMDHTESLDTRRVMLHCELPL
                     NEILVDFNDKIKSISRGYASMDYEHAGYRASKLVKLDLLVNGEPVDAFSTIVHRDRAE
                     PRGRQLASKLKEVIPSQLYQVAIQAAIGGKIVARETISALRKNVTAKCYGGDITRKRK
                     LLEKQKEGKKRMKSIGKINIPQEAFIEVLKSSQ"
     gene            complement(5917..>6345)
                     /locus_tag="VNW28_06740"
     CDS             complement(5917..>6345)
                     /locus_tag="VNW28_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HXA09662.1"
                     /translation="TDLNCLSVMQFAVDILKVRHVIVCGHYGCSGVGAALRRERLGLS
                     DNWLRHVQDVRQKHERAVARAGNDAEAANRLCELNVIEQVANVCQTTIALDAWERGQE
                     LAVHGWIYGLQDGLLRDLNVTVAGAAETTAVYRAAIAALN"
BASE COUNT         1298 a         1886 c         2030 g         1131 t
ORIGIN      
        1 cgacagcacg atcatcacga acccgagcga ttcactcgat cacgcgccaa gtgtgcaaaa
       61 tgtcggctcc cacatgggtc tcgacgcgac ccggaaattg cccggcgaag gttatcaccg
      121 cggctggccg gagctggtgc ggatgaatga agagacgcgc aagctggtcg acgcactgcg
      181 ccgcagccgg gggcgttgac cccggccggg cgaatatcgc ctccgcgtct gccgggggtc
      241 accgcccccg gggctacaac cggatgtcgt tagggccggc ggaaaattcc gttggtcgac
      301 ggatcccgca caagcgcccc ctgcggcacg ccgcggacgt caatcacgtt atgcggagca
      361 tacgggctgt aaacgtaacc cgctgtcgcg gtgggacgcg cgtaaggata gccatccgga
      421 ggagcgtgat aataacgagc ctgatcttcc gcgaccgccg caccgaggag cgctccagcc
      481 aaggctcccg caccggcacc gatcgctgca ccctcgccgg tgccgctggc ggcggcgcct
      541 atcagcgcgc cggtggccgc gccgctaaac gcgccgaaac ccgctccctg gccggccggg
      601 gtttcgcagg aactcagacc ggtcgcgaga agaatcgcgg ccgagaccgc gggcagaaag
      661 tattttttca tagtgctttg gattcgagct gcgcaaatca gacggatgct ccgcccggtt
      721 attcaaggca acttcagcaa ctgccgggtc acatgcaaga gctttgcaaa cttgctcagt
      781 ccgtaatcgc gcgcaaagac gcggaagcga tcctcctcca tttgccgcaa gagggcgcgg
      841 tagatcgagg ccatgatctg cgcgctgacc atcgaacgcc ggtcttcgcg cgggaggagc
      901 gtcgccgcgt tttgaaaata accgtgcgcg cggtccgctt cgaattgcat cagcctaacg
      961 aatcggtcat cgtggatccg tgccgccaga tcctccgggg aataagcaaa gcgggccatg
     1021 tcctcgcgcg gcagatagac ccgaccgctc gccaggtctt tccgcacgtc gcgcaggatg
     1081 ttggtcacct gcaaggcgag cccgagctcg atcgcgtagt cccggcaggc cggattgcgg
     1141 taaccgaaaa tctcaatgct gacgaggccg accgcgctcg cgacccggta acagtactcg
     1201 cgcaaggccg cgaaggtttc gtagtgcgaa atcgtcaggt ccatttccac cccggcgatg
     1261 atctcctcga gcatcgccgg ggtgatggcg tattggccga gcaggccgcg cacggcgggc
     1321 gcgaccggcg actcgcccgg caccgggccg tggagcgctt tgcgccaaac ggtcaggcgg
     1381 cgcgctttct cctccgtggc caggtcggtc gcgtccgcga tatcgtcgat cagccggcag
     1441 aaggcgtaga aggtggtgat gtcgcgccgc cgttccgcgc ctaacgaaac gaaagcgaag
     1501 gcgaggttcg atttgctggc gcgggtgatc tgctcggcgc tggacgagtc ggtcggcatg
     1561 gcgtcagtaa cggatccagc tttcacgatt ggcccgccca atctcgcatt gccggaagaa
     1621 acagacccag ctcgcgagca gccagccggt gaccagcgcg cgcgccaggt cgtaacagag
     1681 tggccagaga ataagggcga gcggccgctc cgtcgccgcg gtgcgaataa cggcatcaga
     1741 aaaggtgagg aaccagtaat gcaaggcgca gatcaggagg aaccagccga agcggaaggc
     1801 atttcgccta acgaattgcc agtgcgcctg gaaagcttcg cgcaaagtct cgttatgcaa
     1861 gacgagcgaa acctgcatcg cggcgaaaga aacgatgagg gcggacatgg cgtaacggcc
     1921 ggcgggcaga taatcgagca cgcccggcac gtcgcgaaaa taggcgatga ggagtggaag
     1981 acgcaggagc agcgtgctcg cgagcacgag cagaccgagc cagcgcagga cgaaactaaa
     2041 acggcgcatg gcaaaacgga gaagcgctcc ttcccggaag tgcagcccct taatccacgc
     2101 caggcagacc gtgatcaggt aaacctgaag gtaaaccgcg cagagatatt cgaagacaaa
     2161 cgccaccgta tcgaccgccg ccgaggtctg gagaagatgc gcgggggaaa gaaagcgcgc
     2221 gcgatccggc agttgccaat agatgagcgg tttgaggagc gaggcgagcg cgccgagtaa
     2281 aacgccgaag taaatcaacc agccgccgag tccgaattgt ttgcggagcg cgcggaccag
     2341 gctgcgatgc aggccgcgcc agttaacgat caggaggagc gcggcaatga aggcgagcgg
     2401 aaaggtcgtc gcggcggcgt cgaagattcc ggcgaccgct tcgaccatgt ggagcaggct
     2461 ctcgcgccaa acctgggaaa agagcggcca atgccacgaa tcccagaagg cgagctggtc
     2521 gaggcgcagg tcgtcggtcg attgcagcgg ggtgaagacc gcgaactgga aggtggaata
     2581 ggcgaaggcg aaaagcgcga aaaggagcca gaggcgcttg aagcgcaggg tgcagcggaa
     2641 gccgtcgcgc agcgcgacgc gcaccgggtt cgtccgcatg acaagcagat agccgccgaa
     2701 catcccgagc agcgggtaga cgcgcggcac ggccgagaac atggggcgac gggaaatttc
     2761 tgcctaacga ataaagcccc agtgccggtt gaacggccag taaacgaaga gcccgcgacc
     2821 gaccagattt tcctccggca ccggccccca atagcggctg tcgtaactgt tatagctgtt
     2881 gtcgcccatc gcgaaatagc tgtgcggcgg aatcacgaaa gagcggtcgg gggcgccgag
     2941 aaacgcctgc cccggcgcgt agcccctgta gggatccttc gccgccatca cccgtcggat
     3001 gccgaaccgg gtggccggct cgccattgat gtagagtttg ggtgaatcga tccgcagctc
     3061 gtcgcccggc gtgccggtga gccgcttgat gtaaaagggc gcgcccgctt ccggatcttc
     3121 gcgaatcccc aggatgtgat tggtgcgaaa gacgaaaacg tcgccccggc tcggaggcac
     3181 gaagttgtag ctgcacttat cgacaaacac ctggtcgccg gtatcgatcg cgccgcgcgc
     3241 aatgacctgg ccgcgacgat agcggttccc cggataaacc tggaaatcct gccgcaaaat
     3301 ttccggcggt gcgtagaccg tgaaggattg gtggtcgcat tcgatgcccg acaaagtgaa
     3361 gaaaaaaaga aacttcttcg aggtgacgtt ctccactgtg ttgtcttcct ccgagacgac
     3421 gttgatgtaa ttgcggccca ggacgaggaa atcgacgatc tgtttcaaaa agttgggcgc
     3481 gggctcgttc gtggggtggc cgatgatacc gttgagcgtg ggctgcatcg agccggtcgg
     3541 aattttgaac ggctggagaa agtaggagcg aatgccaatc gcgaccacga tggcgacgag
     3601 gatgacttcg acgttctcgc gccagacggc gtccttgtgc ggcggggtgt gcatcgtcag
     3661 gagggcgtcg agtcgctccg attcctcgtg gctggcgcga ttgtcgcgcg ccttcatggc
     3721 cgcccgcacc ttctcgatct gggcctggag ctcggcggcg gtggccgggc tcaagcggtc
     3781 gcgccggtaa cgaatcaact tctccgcgtg gcggacgagg agatggcagt gcttgatata
     3841 gcgcggttga aacatgcggc gaaacgctag ccgaggaatg gggtttgcaa agagtattgt
     3901 aggcggcgaa gcgcggcctg agcgacgacg tcgaggcccg cgtcgcccag agggcgacgt
     3961 ctcattgcga cgacttcaag acctcgatga aggcttcctg cggaatgttg atcttcccga
     4021 tactcttcat ccgtttcttt ccttcttttt gtttctcgag cagtttgcgt ttgcgggtga
     4081 tgtcgccgcc gtagcatttt gcggtcacgt ttttgcgcag agcagaaatc gtttcccggg
     4141 ccacgatctt gccgccaatc gccgcctgga tcgcaacctg gtagagctga ctcggaatca
     4201 cttcctttaa tttcgacgcc agttgccggc cacgcggctc ggcgcgatcg cgatgcacga
     4261 tggtcgagaa ggcgtcgacc ggttcgccgt tcacgagcag atcgagcttt accagcttcg
     4321 aggcgcgata gccggcgtgc tcgtaatcca tcgaggcgta gccgcggctg atgctcttaa
     4381 tcttgtcatt aaaatcgacc aggatttcgt tcaacggcag ttcgcagtgc agcatgacac
     4441 gccgggtatc gagcgactcg gtatgatcca tcgtcccgcg cttctcgaga atgagctgca
     4501 ggatgtcgcc gatgcactcg ttagggcaaa gaatgtaagc gcgcacgatc ggctcgcgga
     4561 tttcctcgat cacgctcggg tcgggcaggt gcgccgggtt atccaccagc acgatctcgc
     4621 cgcgcgtcgt gagcacttcg tagatcacgc tcggggacgt cgcgatgata tccatgtcgt
     4681 actcgcgccg gagccgctcc tgaatgattt ccatgtggag caagccgaga aacccgcagc
     4741 ggtaaccgaa gccgagcgcg accgagcttt ccggctggta gacgaaggcg gaatcgttca
     4801 tccgcaactt gctgatggcc gttttcaaat gctcgaagtc cgcggtgttg atcgggtaaa
     4861 ttccgctgaa gaccatcggg tgaatttcct ggaaacccgg caacggcttc tccgcgggat
     4921 ggcgcaggtc ggtcatggtg tcaccgatct tcaactcgga ggtcgtcttg atattggcga
     4981 tcacgtaacc gacatcgccc gcgttgagcg tcgcctgcgc gaacatcttc ggggtaaaaa
     5041 cgcccacttc cttgacctcg tagcgcgtgt cggtgctcat caccttgatc tgttgattgg
     5101 cctctacttt gccggagaaa acgcgcacat aggccaccac gccgcggtag atatcgaaga
     5161 ccgaatcgaa gaccagcgca cgcaagactt catcgccagg ccggggcggc ggcggaatgc
     5221 gatgcaccac cgcctcgagg atctcctcga tcccgatccc cattttcgcg ctgcaatcga
     5281 tcgcttcctc ggcgggaatg gcgagaattt cctcgagttg ccgatggacc gagggcaggt
     5341 cggcgttggg cagatcgatc ttgttgatta ccggcacaat cgtgaggccc tgtttgtgcg
     5401 cgagatgcac gttcgcgact gtctgcgctt ccacaccctg ggcggcgtcg acgatgagga
     5461 gcgcgccttc gcaggccgcg aggctgcgcg aaacttcgta ggcaaaatcc acgtgccccg
     5521 gcgtatcgag caggttgagc cggtattcct ggccgtcctt cgcggtgtag cgcatcgcca
     5581 ccggatgcgc cttgatcgtg atcccgcgct cgcgctccag atccatcgaa tcgagcagct
     5641 ggtcctgcct gtcgcggtcg tggatcgtgc cggtgcgttc gagcaggcga tccgacaagg
     5701 tcgttttgcc gtgatcgatg tgcgcgatga tgcagaaatt gcgagtcagc tcgatcgaca
     5761 tgaaggggca cactatgcgg gcgcgccggg agatggtcaa cggaacggcc cggcggagaa
     5821 attaaccgcc gaggtcgcgg agagcgcaga ggagaaattg gatattgatc tgaactaaca
     5881 tcttctcttc ctcagcgtcc tctgcgacct ctgcggttaa tttaacgccg cgatcgcggc
     5941 ccgataaacc gcggtcgttt ccgccgcccc tgcgacagtg acgttcaggt cgcgcaggag
     6001 cccgtcctgt aatccgtaaa tccaaccgtg caccgccaac tcctggcctc gttcccaggc
     6061 gtcgagcgcg atcgtagtct ggcagacgtt ggccacttgc tcgatcacgt tgagttcgca
     6121 gagccggttg gccgcctcag catcattccc cgcgcgggcg accgcgcgct cgtgtttttg
     6181 tcgcacatcc tgcacgtgac gcagccaatt gtcgctcagg ccgaggcgct cgcgccggag
     6241 cgcggccccc acgccactgc aaccatagtg accgcagacg atgacgtggc gcaccttcag
     6301 gatatcgacc gcaaattgca taaccgacaa acagttgaga tcggt
//
LOCUS       DATMNS010000198         8127 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1932230, whole genome shotgun sequence.
ACCESSION   DATMNS010000198 DATMNS010000000
VERSION     DATMNS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1932230"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1997
                     /locus_tag="VNW28_06745"
     CDS             <1..1997
                     /locus_tag="VNW28_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09663.1"
                     /translation="GLALFLGVVAAILAWMGTAPFARRKVVVRSPEPPLPKSKRARRR
                     MKLPPAVVVATPRPPWHFWAWAVGIVFMMFAVRLFCLLLWVDGNHWKIQSPNNLGDLS
                     LHITYIKYFANGVPLWPDNPIHLLSSLRYPAGFDLFNSLLLLQHIDLISGLVWTGLLG
                     SAATFYGFYRWGGPFGVAGFLFNGGLAGYEFFKKFHFADYQADKLAWKSIPLAMFVTQ
                     RGLLYAIPAGLLLLLHWRRKYYPPATAEIPGAGRGLIPWWVEWSIYATMPLFHVHTFL
                     ALSAVLACWLLIGTWEMRKQIALLLAAAFGPASAIVWMITDHFQAKSILAWAPGWVQG
                     DASFATPAIGLWFVKIPAALGFWIYNFGLMLPAMIALVGVVGWRMWKVVAEGRSLKSR
                     LLVIVPLATIILIFAFFVLADHWGWDIRKVPVWIYCPALALVGVLAWPTLKILQPFRG
                     VIPDSFAFVVPAVAIFVFAIFVKTAPWGWDNTKLIVWAYFIALPFLWSELIARWKLLV
                     RAAVCFVLFASGFVSLFGGLAVGSPGFDLADRTEVDAVATVVRKLPIQARFAAFPTYN
                     HLLLLNGRKVVLGYPGHLWTQGLEYGAIEQQLQALMLGTPDWRERARALHARYLFWGE
                     EEMRAYGSSSQPWRGKAAVVATGSWGTIYDLESRARAAGQ"
     gene            complement(1896..2774)
                     /locus_tag="VNW28_06750"
     CDS             complement(1896..2774)
                     /locus_tag="VNW28_06750"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HXA09664.1"
                     /translation="MKWWLLLAAALTSGCGSLDRAPLPKVATAEAPRPAPGEEAGAAK
                     LRATDVVYFGLTKGSATDRQPAWQIVETWQRSGQRVALGWTELPATRQPSLDRWQRQE
                     ISTQQLLDQLSASERGDWLRRGLRPDLGQAALGSPGNLLRKIRAGETLSAEEHALLPR
                     DYRPRPEAFDNFVDRVSTSSRLRRYDVASLYRAHLAAEQMIAENIVRFMRANPNTKLI
                     VFLPDDAMINPREVADFAAQKAPLRQLILGRPNPPPGTQPQLLTRRPGAAFEVVNRPP
                     GTGRHDRRLAAPRLRA"
     gene            complement(2771..3550)
                     /locus_tag="VNW28_06755"
     CDS             complement(2771..3550)
                     /locus_tag="VNW28_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXA09665.1"
                     /translation="MHLPEFAIIIPACDEAACIGPVLDEFLGAVDPERFVIAVGVNGS
                     SDATAEIARKLPVLVAETERRGYGHGCQAAIDLTKNLFPSIRAYVFCAGDGATDPRDL
                     RSMAAAFEQGYDLVLGSRTMRLRNWRAMTVRHVFANAALGLWCGLLCGRRFSDLGPLR
                     MISRRLFEKIAPQEMTFGWTIEAQIAAAKHDATICEIPVTERRRIGGRQKVSGVTWWR
                     TLSIGCKIVAAGYRTWRDFPPLPAAGKPAFARSNGSRQREA"
     gene            complement(3591..4067)
                     /locus_tag="VNW28_06760"
     CDS             complement(3591..4067)
                     /locus_tag="VNW28_06760"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ family
                     protein"
                     /protein_id="HXA09666.1"
                     /translation="MPTSLTGDREVTSDHFPGLPHRPPFVFVRQLLRCEPGQEAQCSA
                     LFPRDDPMFGGHFPGNPLVPGVILTEALAQTAGIAAASGFPENERPLFLLSAIRTMKF
                     LAAVRPGEEVLLNAEKLAQVGELWQFKVEASVQGKPVAEGQLVLSLTPESSAAALE"
     gene            complement(4045..4512)
                     /locus_tag="VNW28_06765"
     CDS             complement(4045..4512)
                     /locus_tag="VNW28_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09667.1"
                     /translation="MKTRIFACLILLTTLAFTSCPTTNPKKDSKKPATKPAQPSEESA
                     DVDFQAFVGRLRKAVKAHDVNTLASMMTTDFAFVLGGTPAEDRQGPGVFQYWDENNLW
                     IELDGILSERFVPKENYMVAPPQFADPASEYTGYRAGIQRVNGSWKFAYFVNG"
     gene            complement(4570..5337)
                     /locus_tag="VNW28_06770"
     CDS             complement(4570..5337)
                     /locus_tag="VNW28_06770"
                     /inference="COORDINATES: protein motif:HMM:NF024660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HXA09668.1"
                     /translation="MKRDQGIEQEPEHGTEWIKIDLHIHTLDDPRDHLDYTAHQLLGR
                     ARDLGFGALAITLHDTVFDRPEVFADAAAMGILLIPAAEMRLEGADVVVLNVQPDEVE
                     NLCTFADLRRLRARRGASLFCLAPHPFFVFGASIGDRLLQEMDCFDAIEICHFHKGWF
                     DLNRRARQVAAEHGKPLLATSDAHRLHAFGAHYTSIPRPRALTVEEVLRGLREGPHRL
                     TSPSSSWRDLFSVFYFVFVKHPLLKRRASGRSHCCPA"
     gene            5476..5556
                     /locus_tag="VNW28_06775"
     tRNA            5476..5556
                     /locus_tag="VNW28_06775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5509..5511,aa:Leu,seq:gag)
     gene            complement(5662..6894)
                     /locus_tag="VNW28_06780"
     CDS             complement(5662..6894)
                     /locus_tag="VNW28_06780"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HXA09669.1"
                     /translation="MRRPSPWLILVVILLGALILREPRLQQVEDVFLNWFLENSEVVL
                     PPAQVTLVEIGRRDFQRMTPAEEAKPLPKGEAAHRSLSPLEYALFLQAVLEFQPTVVA
                     IEPIVIWPDRDKDQEQVFIDQAMRVPKLLIGIELGGKGKRDLAADDVASFPNVTGKRG
                     ELAEFSGVWRQPDDDIRLISTPGFTNLPNESTDRIRVPMLFGYRGEIVPSFPLQAIML
                     WLRATPADVKVELGSQIILPNGWKIPIQRDGTVTINPVAGQSVRRLTLNELLLAAQEH
                     EKQRPPSINVDNLKDQIVVLRIAEDPLQPPNIFSTAIATIQSNAYVQPTRWAVAWIIV
                     LAAALLSCFLWMISKTNLVLGAILFCAGYGLLNLATIARDHVWLPTFLPCALLSFLVL
                     VRLAGRPPAGPPEIAPTG"
     gene            complement(7093..8034)
                     /locus_tag="VNW28_06785"
     CDS             complement(7093..8034)
                     /locus_tag="VNW28_06785"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HXA09670.1"
                     /translation="MKKIVCLFVLLALTCGVHADDKKKKSAPSKKAPAGGGGGMHMPS
                     ASHGNPAHGLGQTGRTSSGSHAGTSTTNHLKTHSSTSGPASGRTGQAGAASHSSNPLL
                     RKSGQAGSTAHSSNPLTPNNNHQAGAKSTNPLAKKGASQAAMNGHNPNMNGKRAFAAQ
                     NAHGFNNRAGNWQHGAHANYHVRPYGEVFHNYHAVRHDRGWYSSHYDRVVVVGGGYYY
                     WDAGYWYPAWGYDPAFSFYVYDGPIYSYANLPPDQVTMNVQTELQDEGYYTGEVDGQV
                     GPKTRDALGVYQADHNLEVTSAIDEPTVEALGLTGTS"
BASE COUNT         1456 a         2478 c         2571 g         1622 t
ORIGIN      
        1 ccgggctcgc gctctttctc ggagtcgtgg ccgccatcct tgcctggatg ggaacggcgc
       61 cattcgcccg gcggaaagtt gtcgttcgtt cgcccgagcc gccgctgccg aaatcgaagc
      121 gcgcacggcg ccggatgaaa ctgcctcccg cagtcgtcgt cgccaccccg cgtccgccat
      181 ggcatttctg ggcctgggcg gtcggaatcg tctttatgat gttcgcggtg cgcttatttt
      241 gcttgctcct ctgggtggac ggcaatcact ggaagatcca gtcgcctaac aatctcggcg
      301 atctctcgct ccacattact tatatcaagt atttcgcgaa cggcgtcccg ctgtggcccg
      361 acaatccgat ccacctctta agcagcctgc gttatcccgc cgggttcgat ctcttcaatt
      421 cgctcctcct tctccagcac atcgatttga tcagcgggct cgtttggacg gggctgctcg
      481 gctcggcggc gaccttctat ggtttctatc gctggggcgg tccgtttggg gtggcgggct
      541 ttctctttaa cggcgggctc gccggctacg agtttttcaa gaaatttcac ttcgccgatt
      601 accaggcgga caagcttgcg tggaaaagca ttccgctggc gatgttcgtg acccagcgtg
      661 gcttgctcta cgcgatcccg gccggcctgc tcctcttgtt gcactggcga cgaaaatatt
      721 atccgccggc gactgcggaa attcccggcg cggggcgcgg cctcatcccg tggtgggtcg
      781 agtggtcgat ctacgcaacg atgccgcttt ttcatgtgca cacttttctc gcgctctcgg
      841 ctgtgctcgc ctgctggctg ctcatcggca cctgggagat gcgcaaacaa atcgcgctcc
      901 tccttgcggc cgccttcggg cccgcgagcg cgatcgtctg gatgatcacg gaccattttc
      961 aggcgaaatc gattcttgcc tgggcgcccg gctgggtgca gggcgacgcg tcgttcgcca
     1021 ccccggcaat cggcttgtgg ttcgtcaaga tccctgcggc cctcggcttc tggatttata
     1081 actttggcct catgctgccc gcgatgatcg cgctcgtcgg cgtcgttggc tggcgaatgt
     1141 ggaaggtggt agcggagggt cgcagcttga aatcccgact cttggtgatc gttccgctgg
     1201 cgacgatcat ccttatcttc gcgttcttcg tgctggcgga tcattggggc tgggatatca
     1261 gaaaggttcc cgtctggatt tattgccctg ccctggcgct cgtcggggtg ctcgcttggc
     1321 caacgctcaa aatcttgcag ccgtttcgcg gagtcatccc cgactccttc gccttcgtcg
     1381 ttcccgcggt ggcgattttt gtcttcgcca ttttcgtcaa gacggcgccg tggggctggg
     1441 acaacacgaa gctaatcgtc tgggcttact tcatcgcgct gccttttttg tggagcgagt
     1501 tgattgcgcg ctggaagctg ctggtgcgcg cggcagtttg ctttgttctc ttcgcttccg
     1561 gattcgttag tctcttcggc ggcctcgcgg tcggctcgcc cgggtttgac cttgcggacc
     1621 ggacggaagt cgatgcggtt gcgaccgtcg tccgcaagtt gccgatccag gcgcgcttcg
     1681 ccgctttccc gacctacaat catcttctcc tgctgaatgg acgcaaggtc gtgcttggtt
     1741 atcccgggca tctctggacg cagggcctcg agtacggggc gatcgagcag caactccagg
     1801 ccctcatgct cgggacgccc gattggcggg aacgagcgcg ggccctgcat gcgcgttacc
     1861 tcttctgggg cgaggaggag atgcgcgcgt atggctcaag ctcgcagccg tggcgcggca
     1921 aggcggcggt cgtggcgacc ggttcctggg ggacgattta cgacctcgaa agccgcgccc
     1981 gggcggcggg tcaataattg cggttgcgtt ccgggcggcg gatttgggcg accgaggata
     2041 agctggcgca agggcgcttt ctgggcggcg aaatcagcca cctcgcgcgg gttgatcatg
     2101 gcgtcgtccg gtaagaaaac gatcaacttc gtgttcggat tcgcgcgcat aaaccggacg
     2161 atattctccg cgatcatttg ttccgccgcg agatgcgcgc ggtagaggct cgccacgtcg
     2221 tagcgccgaa gacgcgagga ggtcgagacg cgatccacaa aattatcgaa tgcttccggg
     2281 cgcggccggt aatcccgcgg caagagcgcg tgctcctcgg cggagagcgt ttcgccggcg
     2341 cggatcttgc gtaaaagatt tcccggcgat ccgagggcgg cctgcccgag gtccgggcgc
     2401 aagccgcggc gcaaccagtc gccgcgttcc gacgcgctga gctggtcgag caactgttgc
     2461 gtcgaaattt cctgccgttg ccagcgatcg agcgacggct gccgcgtcgc gggaagctcc
     2521 gtccagccga gcgccacgcg ctgcccgctg cgctgccagg tctcgacgat ttgccaggcc
     2581 ggttgacggt cggtcgcgga tcctttcgtc aggccaaaat aaacgacgtc cgtagcgcgc
     2641 agcttcgccg ccccggcttc ctccccaggg gcggggcgtg gagcttcggc ggtcgcgacc
     2701 ttgggcaaag gcgcccggtc gagagatccg cagccgctcg ttagggcagc ggccagcagc
     2761 agccaccact tcacgcttcg cgttgccggc tgccgttgga acgcgcaaag gccggttttc
     2821 ccgcggccgg caagggcggg aaatctcgcc aggtgcgata gccggccgcg acgattttgc
     2881 agccgatcga aagcgtgcgc caccaggtga cgccggacac tttctgccgg ccgccaatcc
     2941 gccgtcgctc ggtgaccggg atttcacaga ttgtggcgtc atgtttcgcc gcggcgatct
     3001 gcgcctcgat cgtccagccg aaggtcattt cttgcggagc gattttttcg aacaggcggc
     3061 ggctgatcat ccggaggggg ccgagatcgc tgaagcggcg gccgcagagc aggccgcacc
     3121 agagacccag cgcggcgttg gcgaacacgt ggcgcacggt catcgcccgc cagttgcgca
     3181 gacgcatggt gcgactgccc agcaccaggt cgtagccctg ctcgaacgcc gcggccatcg
     3241 agcgcagatc gcgcggatcg gtcgcgccgt cgcccgcgca aaaaacgtag gcgcgaattg
     3301 acgggaaaag attcttcgtg agatcgatcg ccgcctggca gccgtggccg tagccccgtc
     3361 gctcggtctc cgccacgagt acgggaagtt ttcgcgcgat ctccgccgtg gcatcggagg
     3421 atccgttcac cccgacggca atgacgaatc tctccggatc cactgcgccg agaaattcat
     3481 cgagcacggg gccgatgcag gcggcctcat cgcaggcggg aataatgatc gcaaattcgg
     3541 gcaggtgcat ctcgtggcgt tccagtgtct aagagtagcg caagccggcg ctattccaac
     3601 gcggcggcag aactttccgg ggtcaaactg agaaccagct gtccttccgc gaccggcttc
     3661 ccctgcacgc tggcctccac cttgaattgc cagagttcgc ccacctgcgc cagcttctcc
     3721 gcgttcagca aaacctcttc cccgggccgg accgcggcaa gaaatttcat ggtgcggatg
     3781 gcggagagca ggaaaagcgg acgctcgttt tcgggaaacc cggaggccgc agcgatcccg
     3841 gcggtttgag caagcgcttc ggtcaggatg acgccgggaa cgagcggatt tcccgggaaa
     3901 tgaccgccaa acatcgggtc gtcgcgcggg aaaagagcgg agcactgcgc ctcctggccc
     3961 ggttcgcaac gaagcaattg cctaacgaaa acaaacggcg gccggtgggg gaggccaggg
     4021 aagtgatcgc tggtcacttc ccgatcaccc gttaacgaag taggcaaatt tccagcttcc
     4081 attgacccgc tgaattcccg cccgataacc ggtgtattcg gaagcgggat ccgcgaactg
     4141 cggcggcgcg accatgtaat tttccttggg cacaaagcgc tccgaaagaa tgccatcgag
     4201 ttcgatccaa aggttgttct catcccagta ttgaaaaacg ccgggtccct gccgatcctc
     4261 cgccggcgtc cctccgagca cgaaggcgaa atcggtcgtc atcatcgagg cgagcgtgtt
     4321 cacgtcgtgc gccttgaccg ccttgcgcag ccggccaacg aacgcctgga aatcgacatc
     4381 cgcactttct tcgctcggct gggccggctt cgttgcaggt ttcttggaat ctttcttcgg
     4441 attagtcgtc ggacagctcg tgaaagccag ggtcgtgagc aggatcaggc aggcaaaaat
     4501 tcgcgttttc ataacggaga agtcgaagcg taggtctgcc acaatcagcg cagcgacaca
     4561 agaccggcgt caggcaggac agcaatggct ccttcccgag gcgcgacgtt tcagcagcgg
     4621 atgcttgacg aaaacgaaat agaagacgct gaacagatcg cgccagctcg acgacggact
     4681 cgttaggcga tgcgggcctt cccgcaaccc acgcagcacc tcctcgaccg tcagcgcgcg
     4741 cggccgcggg atgctggtgt aatgcgcgcc gaaggcgtgc aggcgatgcg catccgaggt
     4801 cgcgagcagc ggctttccat gttccgccgc gacctggcgc gcgcggcgat tgaggtcgaa
     4861 ccagcccttg tgaaagtggc aaatctcgat cgcatcgaag caatccatct cctgcaaaag
     4921 gcgatcgcca atcgacgcgc caaagacaaa aaacgggtgc ggcgcaagac aaaaaagcga
     4981 ggcgccgcgg cgcgcgcgga gccggcggag gtcggcaaag gtgcagagat tttccacttc
     5041 gtcaggctgc acattgagca ccaccacatc cgcgccttcg agccgcattt ccgccgccgg
     5101 gatcaacaag atgcccatgg ccgcggcatc ggcgaacact tccggtcgat cgaaaaccgt
     5161 atcgtgaagc gtaatggcga gcgcgccaaa acccagatcg cgcgcccgcc cgagtaactg
     5221 gtgggcggtg tagtcgagat gatcccgggg atcgtccagg gtgtggatgt ggagatcgat
     5281 cttgatccat tccgtcccgt gttccggctc ctgctcgata ccctggtccc gcttcatctg
     5341 cgccaatcat agatgcagct tccgcgccct gctcggaact tcttcgggga aggagtgggc
     5401 cccaactacg agcacgaagc acgaaagagg acttgcggcg atgcgcgggc tgcgggaaaa
     5461 tcatgcttct ctcccgctcg ggtggtgaaa tggcagacac gtacgtttga ggggcgtatg
     5521 ccgcaaggca tgggggttca agtccccccc cgagcacgcc cctttttgct cttgctcgtc
     5581 tgcgtgctcc tgctcggtct ctcttctccc gcccgctcga ggatttcgat cacgaacacg
     5641 agtaagaggt cgtctggtcc cctaaccagt cggcgcgatc tcgggcggcc cggccggcgg
     5701 gcggcccgcc agcctaacga ggacgagaaa cgagagcagc gcacagggga gaaaggtcgg
     5761 cagccagacg tggtcccggg caatcgtggc cagattgagc aggccgtagc cggcacaaaa
     5821 aaggatcgcg ccgaggacga gattggtctt cgaaatcatc cagagaaaac agctgagcaa
     5881 cgccgccgcc agaacgatga tccaggccac ggcccagcgg gtcggttgca cgtaggcgtt
     5941 gctttggatt gtcgcgatcg cggtcgagaa aatgttgggc ggctggagcg gatcctccgc
     6001 gatccgcaga acgacgatct ggtccttcag attgtcgaca ttgatcgacg gtggccgctg
     6061 cttctcgtgt tcctgggcgg cgagcagcag ctcgttcagc gtcaggcgcc gcacactttg
     6121 cccggccacg ggattgatcg tgacggtccc gtcgcgctgg atcggaattt tccatccgtt
     6181 aggcaaaatg atctgcgaac cgagctccac tttgacgtcg gccggggtcg cccgcagcca
     6241 gagcatgatc gcctggagtg gaaacgaggg cacgatctcg ccgcgatatc cgaagagcat
     6301 cggcacccgg atgcggtccg tcgattcgtt aggcaagttg gtgaaccctg gggtggaaat
     6361 tagccggatg tcgtcgtcgg gctgccgcca gaccccggag aactccgcca gttccccgcg
     6421 tttaccggtc acgttgggaa acgaagctac gtcgtcggcc gcgagatcgc gtttgccttt
     6481 cccgcccagc tcgatgccaa tcagcagctt cggcacgcgc atcgcctggt cgatgaagac
     6541 ctgctcctga tccttgtcgc gatcgggcca aatcacgatc ggttcgatcg ccacgacggt
     6601 cggctgaaat tccaaaaccg cctggagaaa aagcgcgtac tcgagcggcg agagcgagcg
     6661 gtgagcggcc tcgcctttgg ggagcggttt cgcctcctcg gccggggtca ttcgttgaaa
     6721 atccctccgc ccgatttcga ccagtgtcac ctgggctgga ggcagcacga cctcggaatt
     6781 ttccaaaaac caattgagaa aaacgtcctc cacctgttgc aggcgcggct cgcgcaaaat
     6841 gagagcgccg agcaggatga ccacgaggat gagccagggc gaaggacgac gcattggcgc
     6901 agaagctagg gagcgcgcgc cgcaaaaaca aactgaaatt cgcgcggctc tggcttcggc
     6961 tccaggaatc cgtggcttcc cgtcacatga gagaaccgga ggatcgcgac gggtcgaatc
     7021 gccgcttcaa atcgggcgcg aggcctacaa ttgggcagtt tgacgctgat cgcagattgc
     7081 gccgcggccg aactaggaag ttccggtcaa accgagcgcc tccacggtcg gttcgtcgat
     7141 ggcagaggtg acttccaaat tgtgatcggc ctgatagacg ccgagggcgt cgcgcgtttt
     7201 ggggccgacc tgtccatcga cttctccggt gtagtagcct tcgtcctgta actcggtctg
     7261 gacattcatc gtcacctggt cgggcgggag attggcgtag ctgtaaatgg gaccgtcgta
     7321 aacataaaag gaaaaggccg ggtcgtaacc ccaggccggg taccagtaac ccgcgtccca
     7381 ataatagtag cccccgccta ccaccacgac ccggtcgtag tggctgctat accaaccgcg
     7441 atcgtggcgg accgcgtggt agttatgaaa gacttccccg tagggtcgga cgtgatagtt
     7501 cgcgtgtgcg ccgtgctgcc agttgccggc tcgattgttg aagccgtgcg cattttgcgc
     7561 agcgaacgca cgtttgccat tcatgttcgg gttgtggccg ttcatggcgg cctgcgacgc
     7621 gccctttttc gccaggggat tggtgctctt cgcgcccgcc tggtgattgt tgttcggcgt
     7681 gagcggattc gagctatgtg cagttgatcc ggcctgaccg ctttttctca ggagcggatt
     7741 ggaactgtgg ctggccgcgc ccgcctgccc ggttcttcca gacgccgggc cgctggtact
     7801 cgagtgggtt ttcaggtgat tggttgtcga tgtgccggca tgggatccac tcgaggttct
     7861 gcctgtctgc cccaggccgt gcgcgggatt gccatgtgac gcactcggca tgtgcattcc
     7921 acccccaccg ccggcgggcg cttttttgct cggggcggat ttcttttttt tgtcatcggc
     7981 atggaccccg caggtcaagg cgaggaggac gaacaggcaa acgatctttt tcatagagct
     8041 tctctgatgg tcccgagtgc aacaaagaga ggagaccatg gcaaggaaat tcttcgcggg
     8101 ctttgcgcgc tgcctgggtt cgttagg
//
LOCUS       DATMNS010000199         4377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1710479, whole genome shotgun sequence.
ACCESSION   DATMNS010000199 DATMNS010000000
VERSION     DATMNS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1710479"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VNW28_06790"
     CDS             <1..518
                     /locus_tag="VNW28_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09671.1"
                     /translation="AESAIVRTLAPGAYTVIVLGKNVPAGATGVALVDVYDLTPAGST
                     LANISTRGFVGTGNQVMIGGVIAGGTGVAPTNVIVRAIGPSLTPLGVTGALSDPVLQL
                     YDSNGNLTNSNDDWQSDPGAAQVQAAGIAPRDPRESAIFTTITGGGRTAVVSGKDGLT
                     GVGLVEVYYVP"
     gene            complement(603..2843)
                     /locus_tag="VNW28_06795"
     CDS             complement(603..2843)
                     /locus_tag="VNW28_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HXA09672.1"
                     /translation="MSYELESSYRPRGDQGQAIAKLLKSVEAGNRHQTLLGVTGSGKT
                     FTIANVIRALDRPTLIISHNKTLAAQLYSEFKQFFPRNAVEYFVSYFDYYQPEAYIPR
                     SDTYIEKDSSINEEIERLRLSATSALLSRRDTIVVASVSCIYGVTSPEDYLRMLLTVK
                     RGQQISREAVLGRLVDMLYERNDVNFVRGKFRVRGDVVEVYPATADEEAVRLEFFGDE
                     LDAITRFDPLTGHAQESLGVMTFFPAKQFVTPADKLNRALTSIRTELDQRIVQLESQG
                     KLLEAQRLKMRTEYDLEMLQEMGFCNGIENYSRHLSGRPPGSKPFTLLDFFPKDFLLV
                     IDESHATIPQIGGMYEGDKSRKTVLVEYGFRLPSALDNRPLNFPEFMGNANQLIYVSA
                     TPAEFEIKNSVVGNTGYFPHKRARIGEEEPVPFVVAGGNVGQALRLPPGDGPGGAPAV
                     QISRTDQPPDEFDVHTVGAPLVVEQIIRPTGLLDPKVTLKELKNQIDDTIELCRQRVE
                     RQERVLVTTLTKRTAEDLSDYLRDVGLKVRYLHSDIDTIERVEILRGLRAAEFDILVG
                     INLLREGLDLPEVSLVCILDADKEGFLRSQTSLIQTAGRAARHINGEVVLFADIVTPS
                     MQALISISDYRRSKQMEYNEKHGITPQTVRRAVQESLHTILRGREIAAAVIQEAGGDF
                     NLTELLRELEEEMQQASANLEFERAALLRDQIMEVKSGTGLTKIEPKRKPVKYTRGSG
                     RRRSRV"
     gene            2941..>4377
                     /locus_tag="VNW28_06800"
     CDS             2941..>4377
                     /locus_tag="VNW28_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HXA09673.1"
                     /translation="MKSKLFLKITSGLFVFISVARGDTFFVTNLGVNSITKYDASGGN
                     GTPFTSASINGPNGVVLDSAGNLYVSTNANTIEKFAPDGTDLGVFAQTGVNEALGLTF
                     DRSGNLYAANFGGNTVEKFAPDGTDLGVFAVVIRPTGLAFDAAGNLCVANFGNTIARF
                     SPNGTALASFTSLTLNNPEGLAFDSSGNLYAANNGSNTIQYFSPSGVDLGLLVTSGLS
                     APIGLAFDTAGNLYVVNSGNATIGRLVANGGGESTFASTGFSPAFLAVQKTPILINIS
                     TRLNILTGDNILDAGFIVVGSGSKTVLIRGLGPSLTAIGITGALADPIIELHTGSTNA
                     IIAENDNWKNNQQAAIEATGIPPANDAESALIATLTAGAYTVIERGKLNTTGVGLIEV
                     YDLGAGSGPELGNISTRGFVNTGDDVMIAGFIVASASGGSGQVIVRALGPSLASFGVA
                     NPLADPTLELHDGNGTLLAANDNWKTDQE"
BASE COUNT          806 a         1368 c         1354 g          849 t
ORIGIN      
        1 acgcggaatc ggccatcgtg cggacgctcg cgcccggcgc ttacacggtc attgtgctcg
       61 gcaagaacgt cccggccggg gcgaccggcg tcgctctggt ggacgtttac gatctcaccc
      121 cggcgggcag cacgctggcg aacatcagca cgcgcggttt cgtcggcacg ggtaaccagg
      181 tgatgatcgg cggcgtaatc gccggaggaa caggtgtcgc gccgaccaac gtgatcgtgc
      241 gcgcaatcgg accatcgctc actccgctgg gtgtgactgg cgcattgagt gacccggtgc
      301 tgcagttgta tgactccaac ggtaacctga ccaacagcaa cgacgactgg cagagcgacc
      361 ccggagcggc gcaagtgcaa gccgccggga tcgcgcccag ggacccgcgc gaaagcgcga
      421 tcttcaccac catcaccggc ggcggacgca ccgcggtcgt cagcggcaaa gacggcctga
      481 ccggtgtagg gctggtggaa gtctactacg tgccgtaaca ccggtggaaa cgcgccgcga
      541 acgcagcgct tcctgctgcg tgagaagccg gtgcgatttg cgtcttatcg attggtgcag
      601 actcaaaccc gcgagcgccg gcggccggag ccccgcgtgt atttcaccgg cttacgtttg
      661 ggttctatct tcgttaggcc agtgccactt ttgacttcca tgatctggtc gcggagcaaa
      721 gcggcacgct cgaactcgag gttggccgag gcttgttgca tctcctcctc caattcgcgg
      781 agaagctcgg tgagattgaa gtcgccgccg gcttcctgga tgaccgcggc ggcgatttcg
      841 cggccgcgca ggatggtgtg caggctttcc tggaccgcgc gccggacagt ttgcggggtg
      901 atgccatgct tttcgttata ctccatttgc ttgctgcggc gatagtcgga aatcgagatc
      961 agcgcctgca tgctcggggt cacgatgtcg gcgaaaagga cgacctcgcc gttgatgtga
     1021 cgcgcggccc ggcctgcggt ctggatgagc gaggtctggc tgcgcaggaa accctccttg
     1081 tcggcgtcga gaatgcagac taacgagacc tcgggcaggt cgaggccttc gcggagaaga
     1141 ttaatgccga cgaggatatc gaactcggcg gcgcgcaacc cgcgcagaat ttccacccgc
     1201 tcgatggtgt cgatgtcact gtgcaggtaa cgcaccttca acccaacgtc gcgcaggtag
     1261 tcggagagat cttcggccgt ccgtttggtc agggtggtga cgaggacccg ctcctggcgt
     1321 tccacccttt ggcggcagag ttcgatcgtg tcgtcgatct ggttctttaa ttccttgagg
     1381 gtgaccttcg ggtcgagcag gccggtcggg cggatgatct gctcgacgac caacggtgcg
     1441 ccgaccgtgt ggacgtcgaa ttcgtccggc ggctggtcgg tgcgagaaat ttgtacggcg
     1501 ggcgctccgc ctggcccgtc tcccggcggc aagcggagcg cctgccctac atttcctcca
     1561 gcaaccacaa agggcacagg ctcctcctcg ccaatccgcg cacgcttgtg cgggaagtag
     1621 ccggtgttgc cgacgacgct gttcttgatc tcaaactcgg ccggggtcgc gctcacgtag
     1681 atgagctggt tcgcgttacc catgaactcc ggaaagttga gcggccggtt gtcgagcgcg
     1741 ctcggtaacc ggaagccgta ttcgacgagc accgtcttgc gtgatttgtc gccctcgtac
     1801 atgccgccga tctgcgggat ggtggcgtgc gactcatcga tcacgaggag gaaatccttc
     1861 gggaagaaat cgaggagcgt gaacggcttc gaccccggcg gtcggcccga gaggtgccgc
     1921 gaataattct cgattccgtt gcagaagccc atctcctgca acatctcgag atcgtattcg
     1981 gtgcgcattt tcaggcgttg cgcttcgagc aattttccct gcgactcgag ctgcacgatc
     2041 cgctggtcga gctcagttcg gatcgaagtg agggcgcggt tcaacttgtc cgccggggtg
     2101 acgaattgtt tcgccgggaa aaaagtcatc acgcctaacg actcctgcgc gtgcccggtg
     2161 agcgggtcga agcgcgtgat cgcgtccaac tcgtcgccga agaactccag ccgcaccgct
     2221 tcctcgtcgg ccgtcgcggg atagacctcg accacatcgc cgcgcacccg gaattttccc
     2281 cgcacgaaat tcacgtcgtt gcgctcgtaa agcatgtcga cgagccggcc gaggaccgct
     2341 tcgcgggaga tctgctgccc gcgtttcacg gtcaggagca tgcgcaaata atcctccggc
     2401 gaagtgaccc cgtaaatgca ggagacgctc gcgaccacga tcgtgtcccg gcgggaaagg
     2461 agcgcgctcg tggccgagag ccggaggcgc tcgatctctt cattaatcga ggaatccttt
     2521 tcgatgtaag tgtcggagcg cgggatataa gcctccggct ggtagtaatc gaaatagctg
     2581 acgaaatact cgaccgcgtt gcgcgggaag aactgcttga actctgagta gagctgcgcg
     2641 gccagtgtct tgttatgcga aataatgagc gtcggccggt cgagtgcgcg gatcacgttc
     2701 gcgatcgtga acgtcttccc cgagccggtc acgccgagga gggtttgatg gcggttgccg
     2761 gcttcgaccg acttgagcag cttggcgatt gcctgcccct ggtcgccgcg cggccggtag
     2821 ctggattcga gctcgtagga cattcttttc aatatgcgcc atgccggcag gtggcacaac
     2881 gagccgcggt gcgcgtcctg ctctcaaggt cgcgctatct tccttgcacg gtccgcttct
     2941 atgaaatcca agctctttct gaaaatcacc tccgggctct ttgtcttcat ttccgttgcc
     3001 cggggcgaca cctttttcgt gaccaacctc ggcgtcaatt cgatcaccaa atacgacgcc
     3061 agcggcggga acggaacgcc ttttaccagt gcttccatca acggccccaa cggcgtcgtg
     3121 cttgactcgg cgggcaatct ctatgtctcg accaacgcca acacgatcga aaaattcgcg
     3181 cccgacggga ccgacctcgg cgtctttgcg cagacgggag tgaacgaagc gctgggactg
     3241 accttcgatc gcagtgggaa tctttacgcg gccaacttcg gcggcaacac ggtcgagaaa
     3301 ttcgcgcccg acgggaccga cctcggcgtt ttcgcggtcg tcattcgccc gaccgggctc
     3361 gccttcgatg ccgcgggcaa cctctgtgtc gcgaacttcg ggaacacgat cgcccgcttc
     3421 tcgccaaacg ggacggcgct cgccagtttc accagcctga ccctgaacaa tcccgagggg
     3481 ctcgctttcg attcgtcggg caatctttac gccgcgaaca acggctcgaa cacgatccag
     3541 tatttttccc ccagcggcgt ggatctcggc ctgctcgtga ccagcggatt gagcgcgccg
     3601 atcggtctgg ccttcgatac cgccggcaat ctctacgtcg tgaacagcgg gaacgctacg
     3661 atcggacggc tggtcgcgaa cggcggcggc gagtcgacct tcgcctccac cggtttcagc
     3721 cccgccttcc ttgccgtgca gaaaaccccc atcctcatca atatctcgac ccgtctgaat
     3781 atcctgaccg gcgacaacat cctcgacgcc ggcttcatcg tcgtcgggtc cggttcgaaa
     3841 acggtcctca tccgcggcct tggcccatcg ctgaccgcga tcggcatcac cggcgcgttg
     3901 gccgacccga ttatcgaact gcacactgga tcgaccaacg cgattatcgc cgagaacgac
     3961 aactggaaaa ataatcagca ggcagcgatt gaggccaccg gtatcccgcc ggccaatgac
     4021 gccgagtccg cgctcatcgc gaccctcacg gccggcgcct acaccgtgat cgaacgcggg
     4081 aagctcaaca ccaccggcgt cgggttaatc gaagtctacg acctcggggc cggctctggt
     4141 ccggagctgg ggaacatcag cacccgcgga tttgttaata ccggcgacga cgtgatgatc
     4201 gccgggttta tcgtggcgag cgccagcggc gggagcggcc aagtgatcgt gcgcgcgctc
     4261 gggccatcgt tagccagctt cggcgtcgcg aatccgctgg ccgacccgac gcttgagttg
     4321 cacgatggca acggcaccct tctcgccgcg aacgacaact ggaaaaccga tcaggag
//
LOCUS       DATMNS010000200         8049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1713359, whole genome shotgun sequence.
ACCESSION   DATMNS010000200 DATMNS010000000
VERSION     DATMNS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8049
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1713359"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1509)
                     /locus_tag="VNW28_06805"
     CDS             complement(<1..1509)
                     /locus_tag="VNW28_06805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXA09674.1"
                     /translation="MATATLNLTPKLTPKTTAQNESDSPSQSNAHLENTPATGAEYFE
                     IATRATNDAVRDWNVSTGGLTWARGLASLFGYERSPASEEIAFWFNHIHPEDLGRIQA
                     SLRASLASSEERWMGEYRFQRADSEYVYILERALILRDAHGAAVRLVGSLMDVTRRKQ
                     LQAEACRSQRMAAFGQLAGGVAHDFNNFLTTILGYSDLLLSETAVKGRVASQIKEIRD
                     AAGRASALTNQLLAFSRRQALEATVLEVNSLLTNLERSLLRPLGDNISIVCHLHRLKE
                     GAHIKVDAGQLTQIIVNLAVNARDAMPKGGDLTIETSTLKVSPETPPLCAGADLPSGE
                     YVAITVSDHGSGMTDEVKARLFEPFFTTKDDSHHSGLGLATSYGIVRQSGGHICVESA
                     VGEGTTFRIFLPRVAAPPPARYKKAGHRKVVTGTETVLVLEDEKSVRHLSVRVLRNLG
                     YKVLEAAHGDDAKRLITQRGAPKIDLLLTDMVMPEISGRHFADWLQKTSPKTK"
     gene            1629..2456
                     /locus_tag="VNW28_06810"
     CDS             1629..2456
                     /locus_tag="VNW28_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXA09675.1"
                     /translation="MPASSSADPRRPLQNQKALVTGANSGIGEACALALGAAGADVVV
                     NYVSRPEEAERVVGLIRDQGSAALALRADVSREDEVQTMFAGMVKEFGTIDILINNAG
                     LQQDAPFQEMTLQQWATVIGVNLTGQFLCAREAVREFLRRGVVPAVSRAAGKIICMSS
                     VHEVIPWGGHVNYASSKGGVMQLMKSMAQELAPKRIRVNSVAPGAIKTPINRVAWDTP
                     EALTTLLKLIPDQRIGEPEDIAETVVWLASDRSDYITGATIFVDGGMTLYPGFATGG"
     gene            2470..3483
                     /gene="lpxD"
                     /locus_tag="VNW28_06815"
     CDS             2470..3483
                     /gene="lpxD"
                     /locus_tag="VNW28_06815"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXA09676.1"
                     /translation="MNFKLRELAAMCAGEISGSADCEISGAASLAEATPGEITFYGNP
                     RYLAAFRKTRASAAFVPADFSEQIVAAQIRVADPTRAFEQVVRKFAPQPIIFPAGIHP
                     SAVIASGVSLGEKVSIGAHAVIEPGARIGDGTRVGAQSYVGHETTIGAECLVYPNVTI
                     RERSKIGDRVILHSGAVVGSDGFGFEMAAAGHKKVPQIGIVQIDNDVEIGANTTIDRA
                     RFGRTWIQEGAKIDNLVQIAHNVVIGKHSIIVAQVGIAGSVRIGAGVIIGGQAGIIGH
                     IEIDDGTLIGAQTGVSKSLHGGTWWATPAVPLKESMEHLAWVRRLGKLFARVKVLEDK
                     LER"
     gene            complement(3501..4058)
                     /locus_tag="VNW28_06820"
     CDS             complement(3501..4058)
                     /locus_tag="VNW28_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09677.1"
                     /translation="MKRSVLPLLLVVFAFAACETTHRVAVSTFRVVDAPAAYVRRKLD
                     VDEEPPPTTTATTQTNVYTDNNYPPAQPYPAQPPPSSPPPAPAQTQNRIATEERNRQA
                     EPKARPSATPRVTRTESASTAPRTAATSSSSQKPEFPYGKPVPGKPGYVFSPYDKNGG
                     YVDVTGYSPGQKVKDPYTGEIFLVP"
     gene            4361..7717
                     /locus_tag="VNW28_06825"
     CDS             4361..7717
                     /locus_tag="VNW28_06825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA09678.1"
                     /translation="MDDDLPKERTAFIFSDPAGRRWPRLRLTLLFAGIGAFMAAVFFV
                     QTLFVTPQLRLPFGLRQLKGQLKALQKANPAGQLPPNVPLWEKFIATRAAARKQPSPS
                     ATALPASARKSAGGEVRMAFYTNGDPYSYKSLEEHAAQLTHVCPEWFSLVDGLGNMQI
                     DADVRLPKLAAANRIALMPLLTNLVGDAWKPEAVENLAHGPPDRQERFIQNLLTNLRS
                     AKAGGVVLDWEQIDPAYQDDITKLLNKMADALHAEGRQLWLCVQPGQDLDYIDFENVS
                     EKVDRFVALLYDETSEEDSPGPIASRQWFESWLHALLKDADSQQWVIALGSYGYDWTS
                     GTRRAELISFPEAMSRASNAGVEPDTVAPPDHSSDFYYEDGNKEHSVWFLDVVSFLNE
                     LHAVRDQQAGGFAIYRLGTEDSAIWDAINLPRKFQLAGPVKTSLETLLGNETITDVGE
                     GEIVSVDETRSDGARKIEFGPDGYLSATYRGFPEFPTLYHQGAGGEHQVALTFDDGPD
                     PDWTPQVLDILKANGVKAAFFLVGVNAEKYPDLVRRIVAEGHEIGNHTYYHPNLAICW
                     PEHIRLELNATQLLLETITGRSTTLFRPPYNADTSPSSLAELTPLRIANELNYLVVLE
                     NIDPQDWARPGADVILQRIKQQRRDGSIILLHDAGGDRSQTIEALPRIIDWLRTRGDT
                     IVPLSTLIGSSRDAVMPPLSDGQRIPRFVSSAGFRVFHAVEEFLWAFMIVATALIVIR
                     TLVVIFLATRFRRHPAPPGFAPPVSVLIAAYNEEKVIAATLRAVLANDHPGPIEVLVV
                     DDGSADETSAEVEKIAQGDARVRVVRQPNRGKAVALQRGLSLVQHEFIVFLDADTHLQ
                     SDTLRLLVQPLSDEAVVAVSGHAKVGNLRSFIARCQALEYTCGFNLDRRAYDRWNCIT
                     VVPGAISALRRSAIAQAGGLSLDTLAEDTDLTLALHKDNGRIVYTPEAIAWTEAPETL
                     RTLARQRFRWAYGTLQCLWKHRDKVFNWRYRALGWFSLPSVWFFQILLVALTPLVDFF
                     LLASLPFGAWRAVLPFFLIFVAMDVILATLACLLEREPILRAWRIIPMRLIYRPLLSY
                     VIWKAILRALKGAWVGWGKLERTASVPVRV"
     gene            7717..>8049
                     /locus_tag="VNW28_06830"
     CDS             7717..>8049
                     /locus_tag="VNW28_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09679.1"
                     /translation="MMCSRAVFRLAAGLAFCCLSLGCRKQPAAATPVDPQAPVEVVLP
                     EKGAYTGAFVDFGDAEDAVALETIEDFEQMVGKHQAIIASSSYWGEQSFPTANLQMIW
                     RHGSLPLVF"
BASE COUNT         1442 a         2419 c         2595 g         1593 t
ORIGIN      
        1 cttggttttc gggctggttt tttgcagcca gtcggcgaaa tggcggccgc tgatttcggg
       61 catcaccata tcggtcagga gcaaatcgat cttcggcgcg ccgcgctgcg tgatgaggcg
      121 tttcgcgtcg tcgccatggg ccgcttccag cactttgtag ccgagattgc gcagcacgcg
      181 gacggaaaga tggcgcacgc tcttttcatc ctccagcacg agcacggtct cggtgccggt
      241 gacaactttt cggtggcccg cctttttgta gcgagccggt ggaggcgcgg ccacccgcgg
      301 caggaaaatg cggaaagtgg ttccctcgcc caccgcgctc tcgacacaaa tgtgaccgcc
      361 gctctgcctc acgatgccat agctggtggc gaggccgagt ccgctgtggt gcgagtcatc
      421 cttggtggtg aaaaatggct cgaagagacg cgccttgacc tcgtcggtca tcccgctgcc
      481 gtggtcggac acggtgatcg cgacgtactc tcccgatggc aggtcggctc cggcgcaaag
      541 tggcggtgtt tccggcgata ccttgagggt cgaagtttca atcgtcaggt cgccgccttt
      601 gggcatcgcg tcgcgcgcat taaccgccag attaacgatg atctgcgtga gctggccggc
      661 gtcgactttg atgtgcgcgc cttccttcag ccgatggagg tggcacacga tcgaaatgtt
      721 atcgccgagg ggccggagga gggaacgttc gagattagtg aggaggctgt tgacctcgag
      781 cacggtggct tcgagggcct ggcgccggct gaaggcgagc aattgattgg tcagggccga
      841 agcccggccg gcggcatcgc gaatttcttt gatctggctg gcgacccgcc ctttgaccgc
      901 cgtctcggat aggagcagat cgctgtagcc gagaatggtg gtgaggaagt tattgaaatc
      961 gtgtgccacg ccgccggcca gttgcccgaa cgcagccatg cgttgtgagc ggcaggcctc
     1021 cgcttgcaat tgcttgcgcc tggttacgtc catcagcgag cccacgaggc gcacggccgc
     1081 gccgtgcgcg tcgcgcagga tcagggcgcg ttcgagaatg taaacgtatt cgctgtcagc
     1141 gcgctggaag cggtattcac ccatccagcg ctcttccgag ctggcaaggg atgcgcgcag
     1201 gctggcctga atccgtccca gatcctcagg gtggatatga ttgaaccaga aagcgatctc
     1261 ttcgctcgcg ggcgagcgtt cgtagccgaa caggctggcc aggccgcgcg cccaggtgag
     1321 cccgccagtg cttacattcc aatcccgaac cgcatcattc gtagcccggg tagcgatctc
     1381 aaaatattcc gcgcccgttg ccggagtgtt ttcaaggtgc gcattgcttt gggatggaga
     1441 gtcggattca ttttgggcgg tagtttttgg agtcagtttc ggagttaaat tgagggttgc
     1501 agttgccatg cggtcacggc cacggccgca caaatactaa tagctagatc tgtgcccata
     1561 cgccggacgc cctgcgattg aattcccttg cctttccccg ggatcgaaaa tatgcctgcg
     1621 ctcgacgtat gcccgcttcc agctccgccg atcccaggcg cccactgcaa aaccaaaaag
     1681 cgctcgtgac cggcgcaaat tccggcatcg gcgaagcctg tgccctcgcg ctcggcgccg
     1741 ccggcgcgga cgtcgtggtc aattatgtct cgcgtccgga agaggcggag cgcgtggtcg
     1801 gattgattcg ggaccagggc agcgcggcgc tcgcgttgcg cgccgatgtc agccgcgagg
     1861 atgaggtgca gacgatgttt gccgggatgg taaaagaatt cggcacgatc gacatcctga
     1921 ttaacaatgc cgggctgcaa caggacgcgc cgttccagga aatgacgctg caacaatggg
     1981 ccacggtgat cggtgtcaac ctgaccggac agtttctttg cgcgcgcgaa gcggtgcggg
     2041 aatttctccg gcgcggcgtc gtcccggcgg tttcgcgcgc ggcgggcaaa attatctgca
     2101 tgagttcggt gcacgaagtg attccgtggg gcggccacgt gaattacgcg tcgtccaagg
     2161 gcggcgtcat gcagttgatg aagagcatgg cgcaggagct ggcgccgaaa agaatacggg
     2221 tgaacagcgt ggctccgggc gcgatcaaga ccccgatcaa ccgcgtcgca tgggatacac
     2281 ccgaggcgct gaccacgctg ctgaagttga tcccggacca gcgcatcggc gagccggaag
     2341 acatcgccga aaccgtcgtc tggctcgcct ccgaccggtc ggattacatc accggcgcga
     2401 ccattttcgt cgatggcggc atgacgcttt accctggttt cgccactggc ggctaacgaa
     2461 aagtgccttg tgaattttaa gctgcgagag ttggcggcga tgtgcgccgg cgagatatcg
     2521 ggcagcgccg attgcgaaat ctcgggcgca gcttctctcg cggaagcgac tccgggcgag
     2581 attacgtttt acggcaatcc gcgctacctc gcggcctttc gcaagacccg ggcgtccgcc
     2641 gcttttgtgc cggcggattt ctcggaacaa atcgttgcgg cgcaaatccg ggtggcggat
     2701 ccgacccggg ctttcgaaca ggtcgtgcgc aagtttgcgc cgcagccgat tatctttccg
     2761 gccgggattc atccgagcgc ggtcatcgct tccggcgtct cgttaggcga gaaggtgtcg
     2821 atcggcgccc acgcggtgat tgaaccgggg gcgcgcattg gcgacggcac ccgggtcggc
     2881 gcgcagagct acgtcggtca tgagacgacg atcggggccg aatgtctggt ctacccgaac
     2941 gttacgattc gggagcggag caagatcgga gaccgcgtca tcctgcacag cggcgccgtc
     3001 gtcggctcgg atgggttcgg ttttgagatg gccgccgcgg gccacaagaa ggtaccgcaa
     3061 atcggcatcg tgcagatcga caacgacgtc gagattggag cgaacacgac gatcgatcgg
     3121 gcgcgctttg gccgcacctg gattcaggaa ggagcgaaga tcgacaacct ggtgcagatc
     3181 gcgcacaacg tggtcatcgg gaaacactcg atcatcgtcg cgcaagtggg catcgccggc
     3241 agcgtgcgca tcggcgcggg cgtgatcatc ggcggccagg ccgggatcat cgggcacatc
     3301 gagatcgacg acgggacgct gatcggcgcg caaaccggcg tctcgaaaag cctgcacggc
     3361 ggcacgtggt gggcgacgcc ggcggtcccg ttgaaggagt cgatggagca tctcgcctgg
     3421 gtccggcggc tcgggaaact tttcgcccgg gtcaaggtgc tcgaggataa actggagcgc
     3481 taacggccgg gagccactcg ttagggaacg aggaaaattt ctccggtgta gggatccttc
     3541 actttctgcc cgggactgta gcccgtcacg tcgacgtagc cgccgttctt gtcgtaaggg
     3601 ctgaagacgt agcctggttt gccgggcacc ggcttcccat aggggaactc cggcttctga
     3661 gaagaagacg aagtggccgc ggtgcgcggc gcagtcgagg cgctttctgt gcgggtgacg
     3721 cgcggcgtcg cgctgggccg ggctttcggt tctgcttggc gattgcgctc ctccgtggcg
     3781 atccggttct gtgtctgcgc cggcgccgga ggcggggacg aaggcggcgg ctgggccggg
     3841 tagggttgcg ctggaggata attgttgtcg gtgtagacgt tggtttgggt cgtagcagtg
     3901 gtcgtgggcg gcggttcctc gtcgacgtcc agcttgcgcc gcacataggc agccggcgca
     3961 tcgaccacgc gaaaagttga aaccgctacc cggtgcgtgg tttcgcaggc ggcgaaggcg
     4021 aagaccacga gaagcagagg gaggacggag cgtttcatga tggtttggat cctattaaaa
     4081 ttcgatcggt aggacggttt gtgcgccgat aaattcattt cgcagagggc acgggatagg
     4141 ccgccgggct gcgatttcca tcccgatcga ccgcagctgt ggcgaaaagg agagcgaaag
     4201 ggcagatgcg catggatttg gaaaagatag tgttcttcgt gggtgaatgc cagttagtcg
     4261 gcccacgcgg cacccgggaa gttgaagcgg aggcggcgcg tggaaaacga gctttggtat
     4321 ttcccgcgcg accggttagg atatgcgccc caatgctttg atggacgacg atcttcccaa
     4381 agaacgcacc gccttcattt tctccgaccc cgccgggaga cggtggccgc ggctccgact
     4441 gacgctgctt tttgccggca tcggcgcctt tatggccgcg gtctttttcg tccagacgct
     4501 tttcgtcacg ccgcaactgc ggctgccctt tggcttgcgg caattgaaag gccagttaaa
     4561 ggccctccag aaagcgaacc cggccggtca gctcccgcca aacgtcccgc tctgggagaa
     4621 gttcatcgcc acgcgcgccg cggcccggaa gcagccttcg ccatcggcca ccgcgttgcc
     4681 cgcttccgcc cggaaatcgg ccggaggcga agtgcggatg gcgttttata cgaatggcga
     4741 tccctatagt tacaagtcgc tcgaagaaca tgccgcccag ctcacgcatg tctgcccgga
     4801 gtggttttca ctcgtcgatg ggctcgggaa tatgcagatc gacgccgacg tccgcctccc
     4861 caagctcgcg gccgccaatc gcatcgcgct catgccgctc ctgaccaatc tcgtcggcga
     4921 tgcctggaag ccggaagcag tcgagaatct tgcgcacggc ccgcccgatc ggcaggagcg
     4981 tttcatccag aatctgctga ccaatctgcg ctccgcgaaa gctggcggcg tggtgctgga
     5041 ctgggagcag atcgatccgg cctaccagga cgacatcacg aaattactta acaagatggc
     5101 ggacgcgctg cacgccgagg gccggcagct ctggctctgc gtccaacctg ggcaggacct
     5161 cgattacatc gatttcgaaa acgtctcgga aaaggtcgac cgcttcgtcg cgctgctcta
     5221 cgacgagacg tccgaggagg attcacccgg gccgattgcc tcacgccaat ggttcgaatc
     5281 ctggctgcat gccctgctca aggacgccga ttcgcagcaa tgggtcatcg cccttggcag
     5341 ctacggttat gattggacga gcgggacgcg ccgggcggaa ctgatcagtt ttcccgaggc
     5401 catgagccgt gcgagcaacg cgggggtgga gccggacacg gtcgcgcccc cggatcacag
     5461 ttccgatttt tattacgagg atgggaacaa ggagcattcc gtctggtttc tcgatgtcgt
     5521 cagtttcttg aatgagctgc acgcggtccg cgaccagcag gcgggcgggt tcgcgatcta
     5581 tcgccttggg acggaggact cggcgatttg ggacgcaatc aatctcccgc gtaaattcca
     5641 gctcgccggt ccggttaaga cgtcgctcga aacccttttg ggcaatgaga ccatcaccga
     5701 tgtcggcgaa ggcgaaatcg tgagcgtgga tgaaacccgc tcggatggcg cgcgcaagat
     5761 cgagttcggc ccggacggtt atctcagcgc cacctaccgc ggcttcccgg aatttccgac
     5821 gctctatcac cagggcgccg gcggcgaaca ccaggtcgcg cttacttttg acgatggacc
     5881 cgatcccgat tggacgccgc aggtgctcga tatcctgaaa gcgaacgggg tcaaagccgc
     5941 cttcttcctc gtcggggtga acgcggagaa gtatcctgat ctcgttaggc gaattgtcgc
     6001 cgaggggcac gagatcggca accacaccta ctatcatccc aaccttgcga tctgctggcc
     6061 ggaacatatc cggctcgagc taaacgccac ccaactgctc ctcgagacca tcaccggtcg
     6121 ttcgacgacg ctcttccgtc cgccttacaa cgccgacacc agcccgtcgt cgctcgccga
     6181 actcaccccg ctgcggatcg cgaacgaact gaattatctt gtcgtgctcg agaatatcga
     6241 cccccaggac tgggcgcggc ccggtgccga tgtcattctc cagcggatca aacagcagcg
     6301 tcgcgacggc agcatcatcc tgctgcacga cgccggcggc gaccgtagcc aaacgatcga
     6361 ggcgctcccg cggatcatcg attggctgcg gacgcgcggc gacaccatcg tgccgctgag
     6421 cacgctcatc ggcagctcgc gcgacgccgt catgccgccg cttagcgatg ggcagcgcat
     6481 tccccggttc gtcagcagcg ccgggtttcg cgtctttcac gcggtcgagg aatttctctg
     6541 ggctttcatg atcgtcgcta ccgcgctgat cgtcattcgg actttggtgg tgatttttct
     6601 cgccacccgt tttcgtcgcc atcccgcgcc gcccggtttt gctccgccgg tcagcgttct
     6661 gatcgcggcc tataacgagg aaaaggtcat cgccgcgacc ttgcgcgcgg tcttggcgaa
     6721 cgatcatccg ggccccatcg aagtgctggt cgtcgacgac gggtcggcgg acgagacctc
     6781 ggccgaagtt gagaaaatcg cgcagggcga tgcgcgggtt cgagtcgtta ggcaaccgaa
     6841 tcgcggcaag gcggtcgcct tgcagcgtgg cttgtcgtta gtccagcacg aattcatcgt
     6901 ttttctcgat gccgatacgc atctgcaaag cgacacgctg cgcctgttgg tccaaccgct
     6961 gagcgacgaa gcggtggtcg ccgtttccgg ccatgccaag gtcggcaacc tgcgcagttt
     7021 catcgcgcgc tgccaggcac tcgaatacac ctgcggcttc aatctcgacc ggcgcgccta
     7081 cgaccgctgg aactgcatca cggtcgtgcc cggggcgatc agcgccttgc gccgctcggc
     7141 gatcgcgcag gccggcgggc tcagcctcga tactctggcc gaggataccg atctcaccct
     7201 cgctttgcac aaagacaacg ggcggatcgt ttacacgccc gaagcgattg cgtggacgga
     7261 ggcgcccgag accttgcgga cgctcgcgcg gcaacgtttt cgctgggcct acggaacgct
     7321 gcaatgtctc tggaagcatc gcgacaaggt cttcaactgg cgctatcgcg cgcttggttg
     7381 gttcagcctg ccgagcgttt ggttcttcca aatcctgctc gttgcgctca ccccgctggt
     7441 cgattttttc ctcctcgctt cactcccctt cggcgcctgg cgcgccgtcc tgccgttctt
     7501 cctcatcttc gtcgcgatgg acgtgatcct cgcgacgctc gcctgtttgc tggaacgcga
     7561 accgattctg cgcgcctggc ggatcatccc gatgcgcctg atttaccggc cgctcctcag
     7621 ttacgtcatc tggaaagcaa tcctgcgggc gctcaaaggc gcgtgggtcg gctggggaaa
     7681 attggaacgg accgcctcgg tgccggtccg ggtttgatga tgtgttcccg ggcggtgttc
     7741 aggctggccg ccgggttggc gttctgctgc ttatcgttag gctgtcgcaa gcagccggcg
     7801 gcggcgacgc cggtcgatcc gcaggcgccg gtcgaagttg ttctgccgga aaagggcgcc
     7861 tacacgggcg cgttcgtcga tttcggcgat gcggaagacg cagtcgcgct cgaaacgatc
     7921 gaagacttcg agcagatggt cggcaaacac caggcgatta tcgcctcgtc gagctattgg
     7981 ggggagcaga gttttcccac cgcgaatctg caaatgatct ggcggcacgg ctctcttccg
     8041 ctcgttttc
//
LOCUS       DATMNS010000201         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3005877, whole genome shotgun sequence.
ACCESSION   DATMNS010000201 DATMNS010000000
VERSION     DATMNS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3005877"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(587..931)
                     /locus_tag="VNW28_06835"
     CDS             complement(587..931)
                     /locus_tag="VNW28_06835"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HXA09680.1"
                     /translation="MTGTFSDRPGNRDVTLIEAEALKAYENESGNKLSAAEARRNLLT
                     QGVRLNDLVGREFQVGAVRLRGLRLSEPCTHLMRLTHPETLRGLVHRGGLVAEVLCDG
                     ELKVGDEIKDDV"
     gene            complement(1097..2104)
                     /locus_tag="VNW28_06840"
     CDS             complement(1097..2104)
                     /locus_tag="VNW28_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXA09681.1"
                     /translation="MANKPREAMHEIQNDAVTRRTFLGLSLAGGATLLAGGPATIFGA
                     DASTTPSLATNPTWKLGGDLPVNRLGFGAMRITGDGIWGWPADRPNALKVLKRAVELG
                     VNLIDTADAYGPETSELLIAEALHPYPAGLVIATKGGLTRPGPGRWQPDGRPDYLKKC
                     VENSLKRLQLERIDLYQLHRIDPKVPMEDSLGALKEMQSAGKIRHVGLSEVAPEEIER
                     ARKVLPIVTVQNRYNIADRDWEKTLVYCEKEGLGFMPWSPIGGGRGLKPGGALAAVSK
                     AHHASVYQVALAWLLQRSPVMLPIPGTASLAHLEENVAAAKLKLTLEEWKAIDALAGH
                     S"
     gene            2433..2900
                     /locus_tag="VNW28_06845"
     CDS             2433..2900
                     /locus_tag="VNW28_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09682.1"
                     /translation="MRALFLCWIFLGCLLVSCRPHSQAGRVQVGLLDGAPVYHWANFP
                     SGYHDDIARALILREFQRQGFHLPERFVDEAVQEKIQKDYGGDPAKLLAELNLTGETL
                     ANYRQFTGEEIILKAMRKRETIVGHDGHPPISEKEWLTSLRQGARIEMIKGEQ"
     gene            complement(3198..>3761)
                     /locus_tag="VNW28_06850"
     CDS             complement(3198..>3761)
                     /locus_tag="VNW28_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA09683.1"
                     /translation="QRRHGGLGLGLAISKSIADGHGALLEAMSDGRSRGATFRLTIAV
                     VESPATAPARPEKGWSDENGERRGWRILLVEDHADTAAALRNLLARRGHRVSVAHDLR
                     SAREAARGDPFDLLISDVGLPDGTGAELMSQLRTNGMRGIAISGFGMSGDVEKSLAAG
                     FTEHLVKPVNFETLEAAMLRAMRVAAD"
BASE COUNT          697 a         1229 c         1051 g          784 t
ORIGIN      
        1 tcgtccaaaa ttccgggcaa ccagaatttc tagctgtggg tggcggtcat cgacccccgc
       61 accccagact tcctcgaccg cagcctctcc agtccgtcgg cgtgaccgca ggcactcccc
      121 cgttccctga tccccagctc cacttccgcc tttagccttt gctggcgcgg agctggtcga
      181 tcggcgccga atgaaatatg atgaaaccaa atcgcctcgg caggtgtctc aaataacgag
      241 attattttgc gaatcgctcg ccgcgatgcc gatcaagcga aaacatggaa ttgggtcagc
      301 ggcccgcacg aaacggggcc agctgcgatg gacgaatgtc gaattcgccg acttgccttt
      361 agaagggatc gacgagcttt cggagatgca caggccagcg gtggggactt tagcacgaac
      421 gcgcctgcta ctccttgccg ccctccgggt ttgggagttg aaacgcggta ttcgcgatta
      481 ggagcgcgga tggttcgaga acccgaaaat taggtgctct ttctactttc gcctttaaga
      541 gcccggtcgc ggtttcacat ccgtgacggc tacttttcaa tcttcttcaa acgtcatcct
      601 tgatttcatc tcccaccttc agttcaccat cgcagaggac ctcggccacc agcccgccgc
      661 gatgcaccag accgcggagt gtctcgggat gagttaaacg catcagatgc gtgcaaggtt
      721 cgctcaggcg caaacctcgc aggcgcaccg ctcccacttg aaactcccgt cctaccaaat
      781 cattcaaccg cactccttgc gtgagcagat tgcggcgcgc ctcggcggct gagagcttgt
      841 tcccgctttc gttctcgtaa gccttcagcg cctctgcctc gatcaaggtc acatcgcggt
      901 tccccggtcg atcggagaag gtcccagtca tcgctgcata acgatctccc tccaggcctc
      961 ggcccgcgaa cgctctgact cggttcagtg actgtgtcgg cgccgcggcc tgcgccgcaa
     1021 cgtagattcc gagtaactgt cccgatgtct tcatcgtgtg agggtaaccg ctaagtcgcg
     1081 gcccttaatt cttcctttac gaatggcctg ccaaagcatc gattgctttc cattcctcca
     1141 gagtcagctt gagcttggcg gcggcgacgt tttcttccag gtgggcgagc gacgccgtgc
     1201 cgggaatggg cagcatcacc ggcgagcgtt gcagcagcca ggcaagcgcg acctgataca
     1261 cactcgcatg atgcgccttg gacactgctg ccagcgcccc gccaggtttc aatccccggc
     1321 ctccgccgat cggtgaccac ggcatgaaac cgagtccctc tttctcgcaa tagaccagcg
     1381 tcttttccca gtcgcggtcc gcgatgttgt agcggttctg caccgtcacg atcggcagta
     1441 ccttgcgcgc ccgctcgatc tcctccggcg ccacctccga taatccgaca tgccgaatct
     1501 tcccggcgct ctgcatctcc ttgagcgcgc cgagcgagtc ctccatcggc actttcggat
     1561 cgatccggtg caattgatag agatcgatgc gctcgagctg tagccgcttg aggctgttct
     1621 caacacactt cttaaggtaa tcaggtcgac cgtccggctg ccaccggcca gggccggggc
     1681 gggtcaggcc gcctttggtc gcgatcacga gccccgccgg gtaaggatgg agcgcctcgg
     1741 cgatgagcag ctcgctggtt tccggcccgt aggcatccgc ggtgtcgatc aagtttacgc
     1801 cgagttccac cgcgcgcttc aacaccttga gcgcattcgg ccggtccgcc ggccaacccc
     1861 agataccgtc gccggtgatg cgcatcgcgc caaagccgag tcgattgacc ggaagatcgc
     1921 cgcccagctt ccaggtggga ttcgtcgcca ggctcggcgt ggtcgatgcg tcagcgccga
     1981 atatggtggc cggcccgccc gccagcaagg ttgctccgcc ggccagactc aatcccagaa
     2041 atgtccggcg ggtcacggcg tcgttctgta tttcgtgcat cgcttctcga ggtttgttag
     2101 ccatggcgcc caaagtacct ttgacgaaaa cagccgtcaa tggagttgga atgcagattc
     2161 aggacgggcc agaacaaaaa cgcctcattt ttcgcaagag tcgaaaaccg ggagactccg
     2221 cgctgcgggt ttgggagcga aacacggcat ccgaagcggg agcaataact aatcggtttc
     2281 ccgcgtcagc ctccgcctgc tgtcttacat cctcatcccc cggtttcttc cttcatcctt
     2341 ctgccttctt aatttaattc gcccgtcccc gttctccgcc tcccaaattt cagcatgtca
     2401 gcgttcagtt tttcagtatt ttccctcgtt tcatgcgcgc gctgtttctt tgctggatat
     2461 tccttggctg tctgctcgtc tcctgtcggc cgcactccca ggctggccgg gtgcaggtcg
     2521 ggttactcga cggcgccccg gtctatcact gggcaaactt tccgagcggg tatcacgacg
     2581 acatcgcccg cgccttgatt ctgcgtgagt ttcagcggca aggctttcat ctgcccgagc
     2641 gtttcgtcga cgaagccgtg caggaaaaga tccagaagga ttatggtggg gacccggcaa
     2701 agcttctcgc agagctcaac ctgaccgggg aaacgctggc caattaccgg cagttcaccg
     2761 gcgaagagat catcctgaaa gcgatgcgaa agcgagaaac gatcgtcggt catgatggcc
     2821 atcctcccat ctcggaaaag gaatggctga cttccctccg gcagggtgcg cgcattgaga
     2881 tgatcaaggg cgagcagtga cgaacgcggc aagactcaca cccctagagc gtgtcatgaa
     2941 cttgcagagg ctgtagccgc ttcgctgtgc gaagcgtggg tctgcgcgcc gcccaaggca
     3001 attgggcagc gctctccgcg tcgccgacag ggcgacggct acaggacgcc acaggaggcg
     3061 gattttgctc ggccacgcag tctccgcaag ttcatgacac acccctaagc cgccagcgtt
     3121 cgcgaatcag agcggcgcgg ccggcgagac ggcgagaagt tggtcgagcg cggcaaccgg
     3181 cttccgaaaa gcgggcttta gtccgctgct actctcatcg cccgtagcat tgcggcttca
     3241 agggtttcga agtttaccgg tttcacgagg tgctctgtaa atcccgccgc caggcttttc
     3301 tccacgtcgc cgctcatgcc gaagccgcta attgcaatcc ctcgcatgcc gttcgtgcgc
     3361 aactgactca ttaactccgc gcccgtgcca tccggcaggc ctacatcgct gatgagaaga
     3421 tcgaatggat caccccgcgc cgcctcccgc gccgagcgca agtcgtgcgc gacgctgacc
     3481 cggtggcctc gccgcgccag caaattcctc agcgctgcgg cagtgtcggc atgatcctcc
     3541 accaaaagga tccgccaccc gcggcgctct ccgttttcgt ccgaccatcc cttctccggt
     3601 cgcgccggcg cggtcgcggg agactcgacc accgctatcg ttaggcgaaa agtggcgccg
     3661 cgactccggc catcgctcat cgcttccagt aaggcgccgt gtccatccgc gatcgatttc
     3721 gagatggcca atcccaggcc aagaccgcca tggcgccgtt g
//
LOCUS       DATMNS010000202         6932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1220677, whole genome shotgun sequence.
ACCESSION   DATMNS010000202 DATMNS010000000
VERSION     DATMNS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220677"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..361
                     /locus_tag="VNW28_06855"
     CDS             <1..361
                     /locus_tag="VNW28_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXA09684.1"
                     /translation="VTYSAMVHEALAVAKEFSASGVEIEVVDLRSVKPLDTDTVLASV
                     ARTGRLLCVGESFPWGGATAEIIARVAAEGFHLLDAAPARINAKDTPIPYHPNLWSEH
                     RPNAKSIAAKVRQVLAE"
     gene            388..1650
                     /locus_tag="VNW28_06860"
     CDS             388..1650
                     /locus_tag="VNW28_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HXA09685.1"
                     /translation="MPKVPITMPQLGESMAEATIVAIKVQPGEKVVADQEIIEVETDK
                     AVMGVTTPCPGEIAKVDAEVKGVYAVGAVLGYVEASEADAARFVKNAPEPSTGEIAKE
                     VPGREEESTKKQNGSGSHFQVDDTSLPQVAAGSHFQVDQKGVLPVPAGAKGAGYLSPR
                     VKARMAELQLTQADLAAIQGSGNAGRVTIKDLETFLGGLEKQTAEDASAIRTGVADAM
                     RRSWTRPLATVGVSVCLDPVLEDRKKRDPKPGPALYVLRALALALAETPGAASRLIGG
                     RALRSESIDIGVAVEAQDGIMVPVIRNADKTSLADLTSRYNELVDLARRRRVTSEMTA
                     GGIASVSNFGTFGLDWGTPIPLPDQTLLLGLGAGKKAPTWSEEKKEFVPTTQAELTLS
                     FDHRSLDGGGAGRLLQRVTELLSDPAKL"
     gene            1918..2472
                     /locus_tag="VNW28_06865"
     CDS             1918..2472
                     /locus_tag="VNW28_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXA09686.1"
                     /translation="MNSTAAIDPSISMRNLLEQFPGAQRALFRRYHIGGCSSCGFSPD
                     ETLAGVCARNENLDVDEVTDHILASDAADRAMQIEPVELSQRLAAGETIHLLDIRTRE
                     EFETVQLPGAHLFTQESMQEILARWSRTDLLVIYDHQGTRSMDAAAYFQGHGFEKIKS
                     LRGGIDAWSAEVDPKLRRYHLENA"
     gene            2475..2942
                     /locus_tag="VNW28_06870"
     CDS             2475..2942
                     /locus_tag="VNW28_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HXA09687.1"
                     /translation="MSEELERRIEAAVKNPQNLGEMENADAIGTVGSPDCGDMLRMWV
                     KFKEEDGRKVIDRATFQTFGCQTAIAVASVATEMLAGKTVAEAMSMSGEDLAAPLGAL
                     PPVKIHCAQLVEGALRSALGGEVAPAAATAPTLLASFAPSSAPQTKLKIVLDP"
     gene            2960..3517
                     /gene="sufT"
                     /locus_tag="VNW28_06875"
     CDS             2960..3517
                     /gene="sufT"
                     /locus_tag="VNW28_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HXA09688.1"
                     /translation="MHENTEFTLSRDCEAVQIPSGTKTTIPAGTPGVVTQTLGGSYTI
                     ATYQGLARIAEKDLDALGLEKPATNNGATKPVDHAAAPADEKAVWDQLKQCFDPEIPV
                     NIVDLGLVYDCQLIHKPEGGSKVEVKMTLTAPGCGMGPAIAHDAQSKILSIEGVDEAD
                     VQLVWDPPWNQSMISEAGRMKLGMI"
     gene            3649..4176
                     /gene="rplI"
                     /locus_tag="VNW28_06880"
     CDS             3649..4176
                     /gene="rplI"
                     /locus_tag="VNW28_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HXA09689.1"
                     /translation="MPSTQIILTENVSGLGAEADVVRVRRGYARNFLLPTGKAHEVTA
                     RSMRQIDMLKAKRAEREARELNEAEDLARRIGKLKVTFTLHTGETGKAFGSITAHDLA
                     ERLKNEIGRDIDRHSLVLEHPIKTTGEHEVPIKLHHDVTAKFKFNVKSSEEPKPEAPV
                     EEPTEKSRGPHKRTK"
     gene            4177..5673
                     /gene="dnaB"
                     /locus_tag="VNW28_06885"
     CDS             4177..5673
                     /gene="dnaB"
                     /locus_tag="VNW28_06885"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXA09690.1"
                     /translation="MPGDSRGSSRAAEKTRKEPGNGSDRGWPERGGDAERRKLTASSP
                     DIHRALPHSVEAEQGVLGSMLISPREVIAEAVEKINESYFYIPAHQTIYNVLVDLWNS
                     SQGIDLITFTQVLRDRNLLDTVGGASFVTSLFTFVPTAANVGYYLEIVRDKYILREII
                     GACTEGARRSYEEQDEVENLLDEVEQRIYAVGEDRFKGQSLTMKDQVMDAIEAIEKLW
                     EQKGSLTGMATGFIEFDRMTSGLHPSEMIVIAARPSMGKTALAMNIVEHIAVHEKKPV
                     AVFSLEMSSQQLVQRMLCSRARVNLRKARDGFLADSDFDKLTTAAAKLAEAEIYIDDT
                     AGLNILELRAKARRLKSKHNIELIVIDYLQLLRSTSRRGQDNRQIEISEISAGIKGLA
                     KELNIPIIVAAQLNRNPENRTGDSKGRPRLSDLRESGSIEQDADLVGLLVRQEYYADT
                     EEEKDETEGKAELIIAKQRNGPTGDVPLTFRKEFTRFEDRAREEREPL"
     gene            5737..6477
                     /locus_tag="VNW28_06890"
     CDS             5737..6477
                     /locus_tag="VNW28_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXA09691.1"
                     /translation="MNCFPDSLGHGGATFLTTHWSVVLTAQGRSPEALEALNQLCRSY
                     WLPLYAFVRREGHSPEEAKDLTQDFFARLLQRGDFDAVRREKGRLRSYLLISLKHFLS
                     NERQRAGAIKRGHGKQPISLDEILAHQRSEPEPAVTMTAEQIYERRWALRVLEEVLAH
                     LGDEFRASGKTILFERLQDQLVDDPGRSSQAEIGRELGMSENAVKQAFHRLRERYRQL
                     LREEIAHTVATPGEIEDELRHLIAVLRT"
     gene            6494..>6932
                     /locus_tag="VNW28_06895"
     CDS             6494..>6932
                     /locus_tag="VNW28_06895"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09692.1"
                     /translation="MHYPVEMSNDHATGKCRTCRKCGARVLPDAPLGVCSACLLETGL
                     RLLSGEDDPPGRPSTPLLSDFGDYDLLGEIGRGGQGVVYRARQKSLNRMVALKVIGLG
                     HWATETHLKRFRREAEAAASLEHPRITPIYEIGELEGACYFSMK"
BASE COUNT         1506 a         2164 c         2110 g         1152 t
ORIGIN      
        1 cgtgacctac agcgcgatgg tgcacgaagc gctcgcggtc gcgaaggaat tcagcgccag
       61 cggcgtcgag atcgaagtgg tcgacctgcg aagcgtgaag ccgctcgaca ccgatacggt
      121 gctcgcctcc gtcgcgcgga cggggcggct gctctgtgtg ggcgaatcgt ttccgtgggg
      181 cggcgcgacg gctgagatta tcgcgcgtgt cgcggcggaa gggttccatc tgctcgacgc
      241 cgctcctgcg cgcatcaacg ccaaggacac gccgattccc tatcacccga atctctggag
      301 cgagcaccgg ccgaacgcga agtcgattgc cgcgaaagtc cgccaggtcc tcgccgagta
      361 gccatctctc aactctcaac ttctccaatg ccgaaagttc caattaccat gccgcagctc
      421 ggcgaatcga tggccgaggc gacgattgtc gcgatcaagg tacagccggg cgagaaagtc
      481 gtggccgacc aggaaatcat cgaagtcgag accgacaaag ccgtgatggg cgtgaccacg
      541 ccctgccccg gcgagatcgc gaaagtcgat gcggaggtga agggggttta cgcggtcggt
      601 gccgtgctcg gatatgtcga ggccagcgaa gcggacgcgg cgcgtttcgt aaaaaacgcg
      661 cccgagccat ccacgggtga gatcgcgaaa gaggtgcctg gtcgcgaaga ggaatccacc
      721 aaaaaacaaa acggaagcgg ctcgcatttc caagtcgatg acacgagtct gccgcaagtc
      781 gccgccggtt cgcatttcca agtcgatcaa aaaggcgtgc tgcccgtgcc ggcgggcgcg
      841 aagggcgcgg gttatctttc gccccgggtg aaggcgcgca tggccgaact gcaattgacc
      901 caggccgatc tcgccgccat ccagggaagc gggaacgccg gtcgcgtcac gatcaaggat
      961 ttggaaacat ttcttggcgg cctggagaaa caaacggcgg aagacgcgag cgcgatccgc
     1021 accggcgtgg ccgatgcgat gcggcgcagt tggacgcgac cgctcgccac cgtgggcgtc
     1081 tcggtttgtc tcgatcccgt gctggaagat cggaagaagc gcgatccaaa accggggcca
     1141 gcgttgtatg ttctgcgcgc gctcgcgttg gctctggcgg aaactccggg cgcagcctcg
     1201 cgtctgatcg gcggtcgggc gctccgctcc gagtcgatcg acatcggcgt cgcggtcgaa
     1261 gcgcaggacg ggatcatggt cccggtcatt cgcaacgcgg acaaaacttc gctcgctgac
     1321 ctaacgagtc gctacaatga gctggtcgat ctggcccgcc ggcgccgcgt gacttccgag
     1381 atgaccgccg gcgggatcgc gtccgtttcc aacttcggca ccttcggttt ggactgggga
     1441 acgcccattc ctctgcccga tcaaacgctg ctcctcggcc tcggcgccgg gaaaaaggcg
     1501 cccacctgga gcgaggaaaa aaaggagttc gttccgacga ctcaggccga actcacgctc
     1561 agcttcgacc accgcagcct cgacggcggc ggcgcggggc gtttgctcca gcgcgtgacc
     1621 gaactgctga gcgatcccgc gaagctctaa ggcgttgcga cagagtccgg gtagcgcact
     1681 gcctgcccgc cgtgtgcggg cgtctcgcgt gctggttgcg gtgttccaca gcaacgaact
     1741 tttcgtgaag ctcaagcgac tccaggagac caggacatcg agggaaagtt cgcgacggcg
     1801 ggacgccatc gccagcacgc ggaacgcccg cactcggcgg gcaggcagtg cgctacccag
     1861 gccccagccg cagcgcgtct tgcgaaaaag cggcccgccc ttaggtttag tgcgctcatg
     1921 aattccactg ccgcgatcga tcccagcatc tccatgcgga acttgctcga gcaatttcct
     1981 ggggcgcagc gcgccctttt ccggcgctat cacatcggcg gttgcagcag ttgcggcttt
     2041 agcccggatg agacgctcgc gggcgtttgt gcacgcaacg agaacctcga tgtcgacgaa
     2101 gtgaccgacc acatcctcgc cagcgatgcg gctgatcgcg cgatgcagat cgagcccgtc
     2161 gagttgtcgc agcgcctcgc cgccggcgaa accattcacc tcctcgacat ccgaacgcgc
     2221 gaggaattcg agacggtgca actgccgggc gcccatctct tcacccagga gtccatgcag
     2281 gaaattctgg cccgctggtc gcgcacggat cttctcgtga tctacgacca tcaaggcacg
     2341 cggagcatgg atgcggccgc ttattttcaa ggacacggct tcgaaaaaat caaaagcctg
     2401 cgcggcggga tcgatgcctg gagcgcggag gtggatccga agttgcgccg ctaccatttg
     2461 gaaaacgcct aacgatgagc gaggaactgg aaagacgcat cgaagcagcg gtcaaaaatc
     2521 cgcagaacct cggcgaaatg gaaaacgccg acgccatcgg gacggtcggc agccccgact
     2581 gcggcgacat gctgcggatg tgggtcaagt tcaaggaaga ggacggccgc aaggtgatcg
     2641 atcgcgcgac ttttcaaacc tttggctgcc agacggcgat cgccgtggcc agcgtcgcga
     2701 cggaaatgct cgccggcaaa accgtggccg aggcgatgtc gatgtcgggc gaggatctcg
     2761 ccgcgccgtt aggcgcgttg ccgccggtca agattcattg cgcccagctc gtcgaaggcg
     2821 cgttgcgctc cgccctcggc ggcgaggtcg cgccggccgc ggccactgca cctaccctgc
     2881 tcgcatcctt cgctccgtca tcggcccccc agaccaaact aaaaatcgtt ctcgatccct
     2941 agcctaacga ctccgaacca tgcacgaaaa cactgaattc accctcagcc gcgactgtga
     3001 agcggtccaa atccccagcg gcaccaagac cacgatcccg gccggcacac ccggcgtcgt
     3061 cacccaaaca ctgggcggca gctacacgat cgcgacctat cagggcctgg cgcgcatcgc
     3121 cgagaaggac ctcgatgccc tggggctgga gaaaccggcg acgaacaacg gcgcaacaaa
     3181 acccgtcgat cacgccgccg cacccgcgga cgaaaaggcg gtctgggatc aactcaagca
     3241 atgcttcgac ccggagattc cggtcaacat cgtggacttg gggctggtct acgattgcca
     3301 gttgattcac aaacccgaag gcggctccaa ggtggaagtg aagatgaccc tcaccgcgcc
     3361 cggttgcggt atgggccccg ccatcgccca cgacgcccag agcaaaatcc tgagcatcga
     3421 aggtgtcgac gaagcggatg tgcagctcgt ctgggatccg ccctggaacc aaagcatgat
     3481 cagcgaagcg ggacggatga agctcggaat gatttaacga ggatcctgcg ttcgggtccg
     3541 cgtcgcggcc tcggccacaa tgttgacatg aattcgtcgc gccttataat cccgtccctc
     3601 gcgccttcca accggacgga gcgcgtcgga gtgcagtcgt aaattctcat gccttctact
     3661 caaatcatcc tgaccgagaa cgtgtcaggc ctcggcgccg aagccgatgt cgttagagtg
     3721 cgtcgcggtt acgcgcgcaa cttcctcctg ccgaccggaa aggcgcacga agtcaccgcc
     3781 cgctccatgc gccagatcga catgctcaaa gccaagcgcg ccgagcgcga agcacgcgag
     3841 ttgaacgagg ccgaggatct ggcccgtcgc atcggcaaac tcaaggtcac cttcactctg
     3901 catactggtg aaaccggcaa agctttcggc tccatcacgg cccacgacct ggccgagcgc
     3961 ctgaaaaacg aaatcggccg cgacatcgac cgtcactcgc tcgtcctcga gcacccaatc
     4021 aagacaaccg gcgaacacga agtgccaatc aagttgcacc acgacgtgac ggcgaaattt
     4081 aagttcaacg tcaaatcttc cgaagaacca aaaccggaag ctccggtcga agaaccgact
     4141 gaaaaatccc gcggtccgca caaacgaaca aaataaatgc cgggagattc tcgaggctcg
     4201 agcagagccg ctgagaagac tcggaaagaa cccggaaacg ggtccgaccg aggctggccg
     4261 gaacgagggg gcgacgcgga acgccggaag ctcacagctt cttcaccgga tattcaccgc
     4321 gcccttcccc acagcgtcga agcggagcag ggtgtgcttg gctcaatgct catctcgccg
     4381 cgcgaggtta tcgccgaagc agtcgagaaa attaacgaga gctactttta cattccggcg
     4441 catcagacga tctacaacgt cctggtcgat ctctggaact cgagccaggg catcgacctg
     4501 atcaccttca cccaggtcct gcgcgaccgg aacctgctcg acacggtcgg cggagcgtcg
     4561 ttcgtcacca gccttttcac tttcgtcccg acggccgcga atgtcggcta ctacctcgag
     4621 atcgttcgcg acaaatacat cctgcgcgag atcatcgggg cctgcacgga gggcgcgcgg
     4681 cgctcctacg aagagcagga cgaagtcgag aacctgctcg acgaggtgga acaaagaatc
     4741 tacgccgtcg gggaagatcg tttcaaaggc caaagcttga ccatgaagga ccaggtgatg
     4801 gacgcgattg aggcgatcga aaaactctgg gaacagaaag gcagcctgac cgggatggcg
     4861 actggcttta tcgagttcga tcgcatgacg agcggcctgc atccctcgga gatgatcgtc
     4921 attgccgcgc gcccgagcat gggtaagacg gcgctggcga tgaacatcgt ggaacacatc
     4981 gcggtccatg aaaaaaagcc ggtggcggtc ttcagcctgg aaatgagcag ccagcaactc
     5041 gtgcagcgaa tgctttgttc gcgcgcgcgg gtcaaccttc gcaaagcgcg cgacggcttc
     5101 ctcgccgaca gcgatttcga caaactcacg accgccgcag ccaagctggc cgaggccgag
     5161 atctacatcg acgacaccgc gggcctcaac attctggaac tgcgcgccaa ggcccgccgc
     5221 ctcaagtcga agcacaacat tgagttaatc gtgatcgatt acctgcagct gcttcgctcg
     5281 acctctcgcc gcgggcagga taatcggcag atcgaaatct cagaaatctc cgccggcatc
     5341 aaaggactcg ccaaggagct gaacatcccg atcatcgttg ccgcgcagtt gaatcgaaat
     5401 ccggaaaacc gcaccggcga cagcaaagga cggccgcggt tgagcgatct ccgtgaatcc
     5461 ggcagcatcg agcaggacgc cgatcttgtc ggacttctcg ttaggcagga atactacgcc
     5521 gacaccgagg aggaaaaaga cgagacggaa ggcaaggcgg agctgatcat cgccaagcaa
     5581 cgcaacggcc cgaccggcga cgtgccgctt accttccgaa aggaattcac ccgcttcgaa
     5641 gatcgcgccc gggaagagcg cgagccactc tagcgattcg cccggcctgg gaatcgcctt
     5701 gcccccggag ggcgtctttc gttagactcg aagatcgtga actgcttccc ggattcgtta
     5761 gggcacggtg gcgcaacttt ccttacgaca cactggagtg ttgtgttaac ggcccagggg
     5821 cgctcgccgg aagcgctgga agcactcaac cagctctgcc gcagttactg gctgcctttg
     5881 tatgcgttcg ttaggcgaga agggcattca ccggaggagg ccaaggacct gacccaggac
     5941 tttttcgccc gtctgctgca acgcggagac ttcgacgccg tccgtcgcga gaaaggccgg
     6001 ctccgatcct atcttttgat ctcgctcaag cactttctca gcaacgagcg tcaacgcgcc
     6061 ggtgcgatca agcggggcca cggtaaacaa cctatttccc tcgacgaaat tctggcgcat
     6121 cagcgaagcg aaccggaacc ggctgtgacc atgacggcgg agcagatcta cgagcgacgc
     6181 tgggcgttgc gggtcctgga ggaagtgctc gcacatctgg gcgacgaatt tcgcgccagc
     6241 gggaaaacca ttctctttga gcgtctccag gaccagctgg tggacgatcc cggccgctcc
     6301 tcacaagccg aaatcggccg ggaattgggg atgagcgaaa acgcggtcaa gcaggcgttc
     6361 catcgcctgc gcgaacgcta ccgccaactt ttgcgcgagg aaatcgccca cacggttgcg
     6421 acaccgggag aaatcgaaga tgagctgcgg catctaatcg ccgtcctgcg cacgtaaccc
     6481 gggaagaaat ctcatgcatt atccggtgga gatgtcgaac gatcacgcca caggcaaatg
     6541 tcgcacctgt cggaaatgcg gcgcccgggt actgccggac gcgcccctgg gtgtttgctc
     6601 ggcctgcctc ctcgaaaccg ggctgaggct gctttccggc gaggacgatc cgccgggccg
     6661 accgagcacg ccgctgctga gtgacttcgg cgattacgat ttactcgggg aaatcggccg
     6721 tggcggtcaa ggcgttgtct atcgcgcccg acagaaaagt ttgaaccgca tggtcgcgct
     6781 caaagtgatc ggcctgggac attgggcgac cgaaacccac cttaagcggt ttcgccgcga
     6841 agcggaagcg gccgcaagtc tggagcatcc gcggatcact ccgatttacg agatcggcga
     6901 gctcgaaggc gcgtgttact tcagcatgaa ac
//
LOCUS       DATMNS010000203         5328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3365933, whole genome shotgun sequence.
ACCESSION   DATMNS010000203 DATMNS010000000
VERSION     DATMNS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3365933"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="VNW28_06900"
     CDS             complement(<1..1288)
                     /locus_tag="VNW28_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09693.1"
                     /translation="MFRSLFAPGLALFLLLDGPRARAVDAESPNEPTSNLSMLGRKPD
                     WSELEKYQATITHDEFVHLLSDFYCRRGYNPDLIKIEPDAVRILTASGTQNYFLLHFA
                     KSQADRLPLTQWWTAPSALLAPRDARPLAGLHIALDPGHLGGTWAKMEERWFKIGEAP
                     PVQEGDMTLRVAQLLAPRLESLGARVSLVRDKPEPITPYRPSDFEATARQFLEHAGIA
                     SPREDFDGPADPEKEHTVGWQRELLFYRNSEIRRRASLVNFRLQPDLVLALHFNAEPW
                     GDSNHPTLTTNNHLHLLVNGAYLPPELELDDERFEMLQKLLSRAFPEELGLAETIART
                     MAKRTGLPPYQYKTENVTPVGTSGYVYARNLMATRLYRCPTVYLEPYVMNSRDVFVRV
                     KAGDYEGTRRIAGKTRPSIYREYVDGVVDGLLAYFKK"
     gene            1327..2133
                     /locus_tag="VNW28_06905"
     CDS             1327..2133
                     /locus_tag="VNW28_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HXA09694.1"
                     /translation="MLTILFLGDIVGEPGRNAVIARLAELKRAHAIDFTIVNGENAAA
                     GRGITPKITIDLLRAGVSVITTGDHIWDRKEILPFIETEPRLLRPLNYPVGAPGAGTV
                     ILETAKGKVGVINVQARTFMQPILENPFPLLDAAVNAMRAETPVIVVDAHGETTSEKI
                     ALGRYLDGRVSAVLGTHTHVQTADEQIFENGTAFICDAGMCGPVHSILGRAIEPIVGR
                     FVSNLPAPFPVAGGEARLRGVLVEVDETTGRALRITRVDEPGSAVEAPAV"
     gene            2225..3061
                     /locus_tag="VNW28_06910"
     CDS             2225..3061
                     /locus_tag="VNW28_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA09695.1"
                     /translation="MKSLNRHFAAASLLLVLFLPSITFADSKADAYKYAIEGKEAAKK
                     GQWDKAIGAFRKAVEADPKEANNHNNLGIALRSAGKLDEAIKAFSDGIEAEPNNSASY
                     INRGVTYTSQKNYDKAIEDLDRGIKLSPDGVAGYRFRAFAYLATKDYKKAIEDYNVVL
                     KEKDDPAILDRRAFALWNLGEYDKAIADYDTIIKDKPNDKEGYLDRSYVYELKGDLAQ
                     GIADCDKVLSLDPANEDAKNRKARLEYKQQQAVATPTPTARPRRTLPPEATPTPKPKR
                     PS"
     gene            complement(3189..4964)
                     /locus_tag="VNW28_06915"
     CDS             complement(3189..4964)
                     /locus_tag="VNW28_06915"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXA09696.1"
                     /translation="MKKILCAAAVLLVSGFLTANGQTPTQTRPDISNKALLTKPGPSL
                     QGRAEATALHKLADDYYDWRNAQFPVRSSDAGLHNWDDRLTDYSAEKIAARAEYIRAL
                     LARVNAMQTAHWPKDERIDWILFRAQLESAAFDDRVWHSTSRDPQTYVGECSNGIFSL
                     LKKEYDTPRNRALAATKRLLAMPAALAQGEKNLQKPVRLFAQLAIASARAIDPLFNDS
                     LMTLATDLPPNEREELVKARDGALVALHGFADDLEKRLPSMVPFAPMGEKNYDFYLKH
                     VLLLPLDAGQVEMIGRVELARYRALEAWLPDPSLADPDPKRSKNIPPDQISFLKAYES
                     REAEMIQFLKDRRLVTLPDFLGKFEIRQLPEAFKPTSPGGFMNPPGVYDKDSTGFYFI
                     PTYSPDSKNFYIRAAIEDPRPILGHEGIPGHFLQLSIANHLTDEIRRQHDNNVFIEGW
                     ALYGEEMLMREGLYPLDSASQGQVLRLARYRSARIGVDVNLHTGKWNFEQAVKYFMEA
                     GGLDHEAAEGEAAGAASSPTQKISYIVGKWQILDLLGRYRDEQGKNFRLGQFHDALIK
                     NGSLPLSVIKWIMLDDPSDVEEALK"
BASE COUNT         1021 a         1574 c         1647 g         1086 t
ORIGIN      
        1 tctttttgaa ataggcaagc aaaccgtcga ccacgccgtc cacgtattcg cgataaatgc
       61 tcggccttgt ttttcccgcg atccgccgcg tgccttcgta gtctcccgcc ttcactcgca
      121 cgaacacgtc acggctgttc atgacgtagg gctccagata aaccgtcggg caacgataaa
      181 gtcgcgtcgc catcaggttg cgcgcgtaga cgtagccgct cgtgccgacg ggtgtgacat
      241 tctccgtctt gtactggtaa ggcggcagac cggtcctttt tgccatcgtc ctggcaatcg
      301 tctccgccag cccaagttcc tcgggaaatg cgcgactgag caacttctgc agcatttcaa
      361 atcgctcgtc gtcgagttct agttccggcg gcagataggc gccgttcacc agcaggtgca
      421 ggtggttgtt ggtcgtgagg gtcgggtgat tggaatcgcc ccacggctcc gcgttgaagt
      481 gcagggcgag cacaagatcg ggttgcaggc ggaaatttac gagactggcg cggcggcgga
      541 tttcgctatt gcgatagaaa agcagctcgc gctgccaccc cacggtatgc tctttttccg
      601 ggtccgccgg tccgtcgaag tcctcccgcg ggctggcaat tccggcgtgc tcgaggaatt
      661 gccgggcggt cgcttcgaaa tccgacggcc gatagggcgt gatcggctcc ggcttgtcgc
      721 ggaccaacga aactcttgcg cctaacgact ccaagcgcgg cgcgaggagt tgcgccaccc
      781 gcagcgtcat gtcgccttcc tgcaccggcg gggcctcgcc gatcttgaac cagcgctctt
      841 ccattttcgc ccaggtcccg ccgaggtggc cgggatcgag cgcgatgtgg agtcccgcga
      901 gcggtctcgc gtcgcggggc gcgagcaagg cgctcggggc ggtccaccat tgcgtcaatg
      961 gcaggcggtc cgcctggctt ttcgcaaaat gcaggaggaa gtaattctgc gtgccgctcg
     1021 cggtcagaat tcggactgcg tccggctcga tcttgatcag atccgggttg tatccacggc
     1081 ggcagtaaaa atcgcttaac aagtgcacga attcgtcgtg cgtgatggtg gcctggtatt
     1141 tctcgagctc gctccagtcg ggtttgcggc cgagcatgct cagattcgac gtcggctcgt
     1201 taggcgattc cgcgtccacc gcgcgcgcgc ggggcccgtc gaggaggaga aacagtgcca
     1261 acccaggggc gaagagcgag cgaaacatca atttgggatt tttgagttta atacgatgat
     1321 cgcgccatgc tcacgattct tttcctcggc gatatcgtcg gcgaacccgg gcgcaacgcg
     1381 gtcatcgcgc gcctcgcgga attgaagcgt gcacacgcga ttgacttcac gattgtcaac
     1441 ggcgagaacg ccgcagccgg ccgcggcatc acgccgaaga tcacgatcga cttgttgcgc
     1501 gccggagtct cggtcatcac gaccggtgac catatctggg atcgcaagga gattttgccc
     1561 ttcatcgaga cggagccgcg tttgttgcgg ccgctgaatt atccggtcgg cgcgcccggc
     1621 gccggcacgg tgatcctcga gacggcgaaa ggcaaggtcg gcgtgatcaa cgtgcaggcg
     1681 cgcactttta tgcaacccat tctcgaaaac ccgttcccgc tgctcgacgc ggcagtcaat
     1741 gccatgcgcg cggagactcc ggtcatcgtg gtcgatgcgc acggcgaaac gacgagcgaa
     1801 aaaatcgcgc tcggccgtta tctcgatggg cgggtctcgg ccgtgctcgg cacacatacc
     1861 cacgtccaga cggccgacga gcagattttc gagaacggga cggcctttat ctgcgatgcc
     1921 gggatgtgcg ggccggtgca ctcgattctc ggccgggcga tcgagccgat tgtcggtcgt
     1981 tttgtttcga atcttccggc acccttcccg gtggccggcg gcgaagcgcg cttgcgcggg
     2041 gtgctcgtcg aggtcgacga aacgaccggc cgggccctgc gcatcacgcg ggtcgatgag
     2101 ccgggttctg cggtcgaagc gccggcggtt tgattcgcca gcgcggggcc agcttccact
     2161 tgcggagaag attggttgcg accaaagtgc ccgcgcgtcc gtattataag ggaaaggcaa
     2221 cgatatgaaa agtttgaacc gtcacttcgc cgccgcttcg cttttgttgg tgctgttttt
     2281 gccctcgatc actttcgccg acagtaaggc ggacgcctac aaatatgcca tcgagggaaa
     2341 agaggcggcg aaaaaaggac aatgggacaa ggcgatcggg gccttccgca aggcggtcga
     2401 ggccgatcca aaagaagcca acaaccacaa caatctcggc atcgccttgc gcagcgcggg
     2461 gaaactggat gaggcgatca aggcgttcag cgatgggatc gaggcggagc caaataattc
     2521 ggccagctac atcaaccgtg gtgtcaccta cacttcgcag aagaattacg ataaggcgat
     2581 cgaggacctt gatcggggga tcaagctcag tccggacggc gtcgcgggct atcgctttcg
     2641 ggccttcgcc tatctcgcga ccaaggatta caaaaaggcc attgaggatt acaacgtcgt
     2701 cctgaaagag aaggacgatc ccgcgattct cgatcgccgt gcctttgcgc tttggaacct
     2761 gggggagtac gacaaggcga tcgccgatta cgacacgatc atcaaggaca agccgaatga
     2821 caaggaaggt tatctcgatc gcagctacgt ttacgagttg aagggcgacc tcgcacaagg
     2881 aatcgcggat tgcgacaagg tgctgagcct ggacccggcg aacgaggacg cgaagaatcg
     2941 caaagcgcgg ctcgaataca agcaacagca ggcggtcgca acgcccacgc caacggcgag
     3001 gccccgccgg accttgcctc cggaagccac tcccacccca aagccgaaac ggcctagtta
     3061 ggtaagagca aattgcagcc ggggacgccc gcctgtagtt cacggtcgcg ctgctatttg
     3121 gccgcagacc gttcgcccgg ccggagtcgg cggccccggc tccagtgtcg cgccgcgacg
     3181 ttgccccgtt acttcaatgc ctcttccacg tctgacggat catcgagcat gatccacttg
     3241 atgaccgaga gcggcaggct gccgttcttg atcagggcgt catggaattg gccaaggcga
     3301 aagtttttcc cttgttcgtc gcgatagcgg cctaacaaat ccaggatctg ccattttccg
     3361 acgatgtagc tgatcttctg cgttgggctg gaagccgcgc cggccgcttc gccttcggcc
     3421 gcttcgtggt cgagcccgcc ggcttccata aagtatttta ccgcctgctc gaagttccat
     3481 ttgccggtgt gcagattcac atccaccccg atgcgcgccg accgatagcg cgccagccgc
     3541 aagacttggc cctgcgaagc cgagtcgagc gggtagaggc cctcgcgcat cagcatttcc
     3601 tcgccgtaaa gcgcccagcc ttcgatgaag acgttgttgt cgtgttgccg gcgaatttcg
     3661 tccgtcagat gattggcgat cgaaagctgg aggaaatgcc ccgggatgcc ttcgtgtccg
     3721 aggatcggcc gcggatcttc gatcgccgcg cggatataga agttcttcga gtccgggctg
     3781 taggttggga tgaaatagaa gccggtcgag tccttgtcgt aaacacccgg tggattcatg
     3841 aaaccgccgg gacttgtcgg tttgaacgct tccggcaatt gccggatctc aaatttcccg
     3901 agaaaatccg gcagtgtcac cagccggcga tctttcagga actgaatcat ctccgcttcg
     3961 cgactctcgt aagccttgag gaatgaaatc tgatccggtg gaatattctt gctccgtttg
     4021 ggatcaggat ctgcgagcga gggatcgggg agccaagcct ccagcgcccg gtagcgggcc
     4081 agctccaccc gcccgatcat ttctacctgc ccggcgtcga gcgggagcag gagaacgtgt
     4141 ttgaggtaga aatcgtaatt cttctcgccc atcggcgcga aagggaccat cgacgggagc
     4201 cgtttctcga ggtcatcggc aaacccgtgc aacgccacga gcgccccgtc gcgcgccttg
     4261 acgagctctt ccctttcgtt aggcggaaga tcggtcgcga gcgtcatcag gctgtcgttg
     4321 aagagtgggt cgatcgcgcg cgccgaggcg atcgccagct gggcgaaaag ccgcaccggt
     4381 ttttgtagat ttttttcgcc ctgggcgagc gcggccggca tcgccaggag gcgtttcgtc
     4441 gcggcgaggg cgcggtttcg cggcgtgtcg tattcctttt tcaggagcga gaaaatcccg
     4501 ttgctgcatt cgcccacgta ggtctgtgga tcgcgactgg tcgagtgcca gacccgatcg
     4561 tcgaaggccg cactctcgag ttgcgcacga aagaggatcc aatcgatccg ctcatctttc
     4621 ggccagtgcg cggtttgcat cgcgttcacc ctggcgagca gggcgcggat gtattccgcg
     4681 cgcgcggcga ttttttccgc ggaataatcc gttaggcgat catcccagtt atgcagcccg
     4741 gcgtcgctgc tccgcacggg aaactgtgcg ttgcgccagt cgtaataatc gtccgccagc
     4801 ttgtgcaaag ccgtggcttc cgcgcggcct tgcagactgg ggccgggttt ggtgagcaac
     4861 gccttgttcg aaatatccgg cctggtctgg gtgggtgttt ggccgttggc cgtcaagaaa
     4921 ccggaaacca gcagaaccgc cgcggcgcag agaatcttct tcaccgtccc agcgtaagcg
     4981 cccacccgcc cgccgaaaag tccaaagcgc gcgcgggatg ctttcgaatc agtcttgctt
     5041 tcggctgggc gtagtcgcgc gcgagaatta cgaggccaag gccgtattta tggttccatc
     5101 gacgcggcca atgctaatca tttgctaatt ttgctgcaat tagaatagcc aatcgatgcc
     5161 acaacgttcc ctgggtaagt ctgtttccag cttcacccat ccgtctggag ttctgccgtc
     5221 ttagcgcgga aacgttgcgt ccggggagcg cctcggcgcc agggtggcgc gctggcttgc
     5281 gctttagttc gccagttcta acttgcccgc gtgaaaaaaa tcgaggcg
//
LOCUS       DATMNS010000204         2310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1002075, whole genome shotgun sequence.
ACCESSION   DATMNS010000204 DATMNS010000000
VERSION     DATMNS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2310
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1002075"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VNW28_06920"
     CDS             complement(<1..924)
                     /locus_tag="VNW28_06920"
                     /inference="COORDINATES: protein motif:HMM:NF037017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HXA09697.1"
                     /translation="MLKRLLVFLFGRFTWTAPPWLARSGRAVGSHRLVSAASFLLLAL
                     LAGGGWWGWHWYQARPKPLRVAVIADPIPVTKLEKDLKPAPLHVRFSASVARLEQIGK
                     EVSRGVHLDPNAEGKWSWTSDAELTFKPKQDWPAEQKFRVTFEKNFFPRRILLEREEV
                     EVTTPPFTDVVQEIQFYQDPKDPAIRQVVATLAFTHRVDRRDLEKHIALSMIGGSNVF
                     ADKPAPFFAVTYGLHDRLAYVRSVPLALPEHEDFMKIVVSKGVATTQGGAQTASDVDD
                     KVRVPDAFSFFKIDESNARVVRNADGDPEQVL"
     gene            1029..1649
                     /locus_tag="VNW28_06925"
     CDS             1029..1649
                     /locus_tag="VNW28_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXA09698.1"
                     /translation="MSRAEFLATRRSLVERLADWNDRKRWQQFFDSYWKLIHRAARQA
                     GLTDSEAQEVVQETLITVAKKIDQLKYDPALGSFKGWLLNITRWRIADQFRKRKRAGE
                     QSAKTRRDEAETATIERIADPAGFDLDAAWDEEWHRNLLQAATERLKRKVSGKQYQIF
                     DCYVLKQWPVRKVAKELRVSVAQVYLAKHRLGALLKREVARVERAR"
     gene            1776..>2310
                     /locus_tag="VNW28_06930"
     CDS             1776..>2310
                     /locus_tag="VNW28_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXA09699.1"
                     /translation="MRIPSIPDHEMLGIIGRGAYGEIWLARSLTGALRAVKVVYRDTF
                     ETERAFNREFEGMSAFEPISRAHDGFVDILHVGRSVAGGFFYYVMELADDQVSGAEIE
                     ITRYTPKTLKAELGHTLPADECLRLGLLLAEALGALHRAGLAHRDIKPSNIIFVQGAP
                     KLADIGLVATSGQRSFVG"
BASE COUNT          487 a          670 c          739 g          414 t
ORIGIN      
        1 caggacttgt tccggatcgc catccgcatt gcgcacgacg cgggcgttgc tctcgtcgat
       61 cttgaaaaaa ctgaaggcgt cgggcacgcg caccttgtcg tccacgtcgc tcgcggtctg
      121 cgcgccgccc tgcgtggtcg cgacaccctt gctgacgacg attttcatga agtcctcgtg
      181 ttccggcaac gcgagcggca cgctgcggac ataagcgagt cgatcgtgca gcccgtaagt
      241 gactgcgaaa aacggcgccg gcttatccgc aaaaacgttc gagccgccga tcatggaaag
      301 ggcgatatgt ttctcgagat ctctgcgatc gacgcggtgg gtgaaggcca aagtcgcgac
      361 gacctgccgg atcgcgggat ctttcggatc ctgatagaac tggatttcct ggacgacgtc
      421 ggtgaacggc ggggtggtca cttccacttc ctcgcgctcg agcaggatgc gcctggggaa
      481 aaagttcttc tcaaacgtaa cgcgaaactt ttgttcagcc ggccagtcct gtttcggctt
      541 aaaagtgagt tccgcatcgg aagtccacga ccacttgcct tctgcgttag ggtcgaggtg
      601 aaccccgcgc gagacttcct tgccgatttg ttcgaggcga gcgacggaag cgctgaaacg
      661 aacgtgcaac ggcgccggtt tgagatcctt ctccaatttc gtgaccggaa tcggatcggc
      721 gatgaccgcg acgcgcaacg gttttgggcg cgcctgatac caatgccagc cccaccagcc
      781 gccgccggcg aggagcgcga gaaggaggaa cgacgccgcg ctgaccaggc gatgcgatcc
      841 gacggcgcgg ccgctccgcg cgagccacgg tggcgcggtc caggtgaagc gaccgaacaa
      901 aaatacgagg aggcgtttga gcataaacga actggatgtt gcggtgaata cggggcgcga
      961 ggcgaggttc tttcaacgat ttgcaaaaaa cttcacgcgc attgggatcc gcgataaaac
     1021 ggagaggcat gagtcgcgct gaattcctcg ccacgcgtcg gagcctggtc gagcggctgg
     1081 ccgattggaa cgaccggaaa cgctggcagc agttcttcga ttcctattgg aaactcatcc
     1141 accgcgccgc gcgccaggcc ggcttgacgg acagcgaggc ccaggaagtg gtgcaggaaa
     1201 cgctcatcac ggtggcgaag aagatcgacc agctgaaata cgatccggcg ctcggatcat
     1261 tcaaaggctg gctcctgaac atcacgcgct ggcggatcgc cgatcagttt cgaaaacgaa
     1321 agcgcgccgg cgaacaatcc gccaaaacgc gacgcgatga agctgagacc gcgacgatcg
     1381 aacggattgc cgatccggcc ggcttcgatc tcgatgcggc gtgggacgaa gaatggcatc
     1441 gcaatctgct ccaggccgcg accgagcgcc tgaaaaggaa ggtaagtgga aagcagtatc
     1501 agatttttga ttgctacgtc ctgaagcaat ggccggtgcg gaaagtcgcg aaggaactgc
     1561 gggtgagcgt cgcccaagtg tacctggcca aacatcgcct gggcgcgctc ttgaaaaggg
     1621 aagtggcgcg tgtggaacgc gctcgttagg gctctctcct cgctatcgcg ctcgtgctca
     1681 tgatcgtgat cggcaaaaga gtcgattgcg attgcgagta cgagcacgac ggcggtcatg
     1741 gaccgccgct agagaagaag gtataacgac aaaacgtgcg cataccgtcg attcccgacc
     1801 acgagatgct gggcatcatc gggcgcggcg cgtatggcga aatctggctg gcacgcagcc
     1861 tcaccggcgc gctgcgggcg gtgaaggtag tgtaccgcga cacgttcgaa accgagcgcg
     1921 ccttcaatcg cgagttcgag ggaatgtcgg ccttcgagcc gatctcgcgc gcgcacgacg
     1981 gcttcgtcga tatcctgcac gtcggccgga gcgttgccgg cggctttttc tattacgtga
     2041 tggagttggc cgacgatcaa gtcagcgggg ccgagatcga gatcacgcgc tacacgccaa
     2101 agacgctgaa agcggaattg ggccataccc tgccggcaga cgaatgcctg cgcctcgggc
     2161 tcctgctggc cgaagcgctc ggcgcgctcc atcgcgccgg ccttgcccat cgcgacatca
     2221 aaccttcgaa catcattttc gtgcagggcg cgccgaagtt ggccgacatc gggctggtcg
     2281 caaccagcgg gcagcgctcg tttgtcggga
//
LOCUS       DATMNS010000205        14529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_288019, whole genome shotgun sequence.
ACCESSION   DATMNS010000205 DATMNS010000000
VERSION     DATMNS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 14529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14529
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288019"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..2948
                     /locus_tag="VNW28_06935"
     CDS             162..2948
                     /locus_tag="VNW28_06935"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXA09700.1"
                     /translation="MPASTVSTICTICGEPLQGRSDCLVCLLRGGLDRPNETASSPAF
                     GEFEIERRADGTLWELGHGAMGVTYRARDQVLHRDVALKVIDVPAGTGGAKTARERFL
                     REARATAALRHPNVAGVFQFGDSSEIDRCYYAMELVEGETLEALVRREGPVSADAALA
                     IAIQVTRALVAAAAHGLIHRDLKPANIMLVPNDSGAAAVEVKVIDFGLAKATAGVTEM
                     DLTHGAFVGTPTFASPEQFAGKPADARSDIYSLGMTLWYALTGEVPYPGRTIEEIRAS
                     RAQVALPVEQLAARKIPTPVIKLIRRILAADPPARPQSARALLAELENCRTDMQTTPR
                     RRRRRTAAVVIGLLVVSLAGLTNYRWRQPPPPAAAPREKSIAVLPFENLSKDETNAYF
                     AAGIQDDVLTSLAKIHAFKVISRTSAMAYEKPDGRKMREIGRVLGVENVLEGSVRREG
                     NRVLLNVQLIDARQDRHIWAERYDRTEADSIGLQGELATQIAAALEAKLAPEEVARLA
                     VRPTDNPEAYTLYLRALGRERAANRSTEDLDDAEHLYEEAIKLDPRFALAHARLSIVE
                     SSIVGMEGDPIRKAKARREAEEAIRLAPRLGEAHMALGLSLYWADKNYDAALKEFSTA
                     AATAPNEADIFNFIAGIYRRQGRWRDSLAAYQRALDLDPRDRRMLCLAAIDYTLVRDW
                     SAASACYHRALEIAPDSAPARIGLAWLEVFRNGDPAAGRKILQELPPGIDPDGEVTAA
                     RWDLAMLERDYTSAEKILTDFPSAEFPHVGREPKTFYLGRVALARGDTASAQRNFAAA
                     VPGFEEWIRESPNSASPHGRLGLLYAYQGRKEDALREGRRAVQLEPESKNAFHGAVAA
                     AELALIYARTGDADQAISLIDRLLATPGPAQPGFPENITLADLRLRWEWDPLRSDPRF
                     QKILAGPEPKTVYR"
     gene            complement(2997..3431)
                     /locus_tag="VNW28_06940"
     CDS             complement(2997..3431)
                     /locus_tag="VNW28_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HXA09701.1"
                     /translation="MFLVDTNVLVDVVNRDSEFHEPCRRTIEACRARPAPWYLSWPIC
                     YEFLRVCTHPRILPHPWSIGAAWHFLETLLDSPSAGMLLPTTQHAALLNEIIDAAPQL
                     RGNILHDLHTATLMREHGIKEIYTRDMDFHRFPFLTVLDPAA"
     gene            complement(3432..3680)
                     /locus_tag="VNW28_06945"
     CDS             complement(3432..3680)
                     /locus_tag="VNW28_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HXA09702.1"
                     /translation="MVSHLKTTLNIDDTVMAQLRREAARQNRTMSELVEAALRLLFHR
                     GKLPQKKKLAPLPRFHGGKPLVEIADRNALYDAMEERR"
     gene            3770..5827
                     /locus_tag="VNW28_06950"
     CDS             3770..5827
                     /locus_tag="VNW28_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA09703.1"
                     /translation="MDPRKFFAELQRRNVYRAGVIYAMSAWLLIQVATQVFPFFEIPN
                     WVVRSIVVVLVLGFPVSLALAWAFELTPEGLVKTEDVPLGKSIRWKTGRKLDFVIIGI
                     LTVAILFLLFLHFSPSHRATRDPAIPEKSIAVLPFENYSAEKENAFFADGIQDDLLTN
                     LAKIKALTVISRTSAMKYRDVSARDIREIGKSLGVANVLEGSVRREGSRVAVNVQLID
                     AFNDRHIWAHRYDRTIADSLGLEGELATEIADALRAQLTPDEKVRVAKKPTGNADAYV
                     LYLRALPYEQGPDTLLQDYRQAVELYTKAIALDPEFALARAHLASTCAEIFHFHEPLD
                     SWKARARSEAEAALRLQPDLGEAHFALGQCAYWFDADYERALAEFAIAQRALPNDSNI
                     GAYVAAIRRRQGRWEEALATYERMEKLDPQNPNIIRNVLFTNTSMRRWPQAAEAAARF
                     RAVAPDSVVAKIQSGYVEFMWKGDIGALQRWLGDIPPTDDPDGIVTCVRWEGAMLHRD
                     FVGAADILAKSPTRELSYLSGGLQPQSFLLGITAVASNDRAGAARNFAAAQPEVEKAV
                     QEAADAPACHANLGLLYAFMGKHDEAIREGRLAVELKPIAKDANDGAIVLCYLALIYG
                     RVGEKDQAIPLIDRLLKTPGAVDSVNYSITLNDLKYRWEWDPLRGDPRFQKLVGQTAP
                     PPK"
     gene            complement(5858..6631)
                     /locus_tag="VNW28_06955"
     CDS             complement(5858..6631)
                     /locus_tag="VNW28_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HXA09704.1"
                     /translation="MKNIVVVGYPKSGCTWATRLVAELVGCPVAGFWRLEKKEIAIEG
                     ENRVSDFRCYKSHHQLAELLLPVDEVKKNVIYVLRDPRDVAISGAHHFTFDPFPRLAR
                     LFHGGRLKKIYQRTLYPLVASQDYRLGRMTTALLEGCAQVHNWCRVSWREHWQPYQEA
                     GVPIVRYEDLLANPGEEARRILHHLGIERTSEEIATAVHNQSFARKKETLLQSGETGR
                     AKFLRVGKGGQWREKLPADLQRRFSEELRAELAAWHYPA"
     gene            complement(6648..8162)
                     /locus_tag="VNW28_06960"
     CDS             complement(6648..8162)
                     /locus_tag="VNW28_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HXA09705.1"
                     /translation="MHSPIITPAQMRAAEEAAFARGITAEALMDQAGERIAGALTRFF
                     PRPGKCIVFAGKGHNAGDALVAARWLAEAGWKIETRLTFSEKELSPLTSEKLGALRDQ
                     ECGRDLGAPKGVTPGIGAPRPRPHSQTIILDGLLGLGAHPPLREPIRSACREINSLRA
                     GAYVVAIDLPTGLDGDSGAADSECVRADFTITVGAAKRGLVADSAIDFVGRLEVVLLD
                     ELRAEDPGAGEAAIAEVLRDLLPRRRYSAYKNQFGRIGIVAGSRGFTGAAVMCSLGAL
                     RAGAGLVELFVPEEIYEIVATASTPEVMVKPVKSYAALLEEPIDVWAVGPGLGKARAA
                     EILALIRAAQQPMVVDADGLNILAERRAMLDELPGARLLTPHPGEMQRLFPTEKMSRA
                     EIATNFCAQHAVTLLLKGSRTIVAEAARPLSYNTTGNPGMATGGMGDVLTGVCAALLG
                     AKLAPRDAARLGAWLCGRAAELAVFNRHASEESLLPGDVLDHLGAAFSDLRETR"
     gene            complement(8164..9006)
                     /locus_tag="VNW28_06965"
     CDS             complement(8164..9006)
                     /locus_tag="VNW28_06965"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXA09706.1"
                     /translation="MAEKRLRAPKRAPLFALVAIALSLLPAPAWSASSNLPTVVIDAG
                     HGGFDRGGIPGQAVAEKSMTLDVALRLRRILHDAGYRVVMTRDSDVFVTLGDRVRIAN
                     SYGNAIFVCIHFNAATRAGANGIETYYYSNQSAALAANIHRQVVAGTTSENRGIRRRG
                     YFVLRRTSIPAVLVECGFLTNPTEARLALQSSYREQLAQRIANGVMNKRAPPNRAVVA
                     GLTHVVPPVEQPFNTNDFVRAEPERTGSSHHSSKSSKKSSRHKKSSTSTTKKKKKKTP
                     ESDE"
     gene            complement(9012..9875)
                     /locus_tag="VNW28_06970"
     CDS             complement(9012..9875)
                     /locus_tag="VNW28_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09707.1"
                     /translation="MTRLLALAVLFLATITARAQIEVDLKFKRLQYIAYEPVIATVTI
                     TNLAGREIELRDDTGQHWYGFEVTANEGRTLAPLRQRSEPPLKIAAGTSVTRKIDLTP
                     LFPITDRGVYHARANVYFADLNKFFYARAKVFEVTNARPIWQRTVGNPGADGVRTYSL
                     LTNRFPDHTSLYVRVEDKANSLVYATYSLGRLISFDEPHAEVDRNNQLHVLQCSAPRL
                     WSYSVVGLDGRLAKHTTYTQTRTSPHLRRAVDGTVAVAGGMVDAPVAPLASGKEGPKL
                     SDRPADLPLEN"
     gene            complement(10100..11239)
                     /locus_tag="VNW28_06975"
     CDS             complement(10100..11239)
                     /locus_tag="VNW28_06975"
                     /inference="COORDINATES: protein motif:HMM:NF024806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA09708.1"
                     /translation="MRAVSFLQGSRSQQTFVAAMALLVAVAVAQILSALYLSTRHPGE
                     APSVASQNAAVARLFKQSLPAPSAAPVTVPAVEGVPQATVRRGQAPPSSVAETLLRVA
                     RGFRDRGDTTNAIAKLQQATALDPGNPEILAELALTYESMQLFDRSNEVWRRLQSLGP
                     AVGPLYELAEVKLRVGVPAQSNALRANGSTAPPGAPPSDAAGIPDGSTFGISEVTLKT
                     EPEPDAETKLLLRVGVKARPDTPIDHTKVKIQVFFYDTVNNDQVVLTNADVSYEWVTP
                     GHDWADTGTEVLDVTYLRPKRNEPAPGTALANQPVEVPDSVEKAKLAARKPSPPALLP
                     PSESGPRAYLGYIVRVYYQDQLQAVRAEPARLLNLFPPPVTAPPQ"
     gene            complement(11380..11850)
                     /locus_tag="VNW28_06980"
     CDS             complement(11380..11850)
                     /locus_tag="VNW28_06980"
                     /inference="COORDINATES: protein motif:HMM:NF040320.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09709.1"
                     /translation="MAQSLPETTSKIDGPLVRQFSVFLPNKVGALLDIVKLLSARNTH
                     LVALSIAESTDSAIARIVVSDPETAENIFLEHNVPFGVCALVVVELREVATEVAKLLA
                     ALLMAEVNMHFSYPLLTRPHGRAALVLHVDDTECACSVLQGEGFKLLSQADISR"
     gene            12043..13428
                     /locus_tag="VNW28_06985"
     CDS             12043..13428
                     /locus_tag="VNW28_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXA09710.1"
                     /translation="MKQLPKAKIDEFKAHFRGEVLLPGDVNYNEVRQIWNAMIDRRPA
                     LIARCTSPQDVVHSVKFAREQDLLVSIRGGGHNIAGNAVCNDGLMIDLSLMKGVQVDP
                     NARRATVEPGCTLADFDAVAQTHGLATPLGINSTTGVAGLTLGGGFGWLSRRYGMTID
                     NLLSANVVMADGSQVRASETENAELFWGLRGGGGNFGIVTSFEFRLHPVGPNVLSGLI
                     VFPFDQAKSVITQFARFTETMPEELNVWMVTRKAPPLPFLPEAVHGKEIVALALCYAG
                     DPREGEKLIAPLRGFGTAHGEHVGVQPYVAWQQAFDPLLAKGARNYWKSHNFSRLSDG
                     AIDAIIEYAGRLPSPHCEIFIGTIGGQTTRVAPDAMAYSSRDANYVMNVHGRWESAAE
                     DEGVITWAREFFAKSKPFASGGAYINFLTEEETDRIESAYGPVYNRLVALKKKHDPTN
                     FFRMNQNIKPA"
     gene            13470..13856
                     /locus_tag="VNW28_06990"
     CDS             13470..13856
                     /locus_tag="VNW28_06990"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HXA09711.1"
                     /translation="MGRGPITGGCYCGQVRYRAKQEPISHANCHCANCRRAAGAQSVA
                     WITVAHSAFAFEKGSPRRYRADTGAWRTFCDQCGTSLTYESDTRPGEIDLTTGSLDHP
                     EDFPPNKDVFPEEKLPWVSLVDETRC"
     gene            14101..>14529
                     /locus_tag="VNW28_06995"
     CDS             14101..>14529
                     /locus_tag="VNW28_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09712.1"
                     /translation="MTHRQHFAIAGIFLLLATKAGAFDFEFARATSSAGQFSVVLPKP
                     FSELPANAGVNSAAAIKPKQSFVIGGKPAPRVVFIATKMVYGSTSDARSVVTSISGGE
                     PPGFRRAYIKKVQVAGLPGLEIKSLSPSTVGYRRVLLAGDT"
BASE COUNT         2759 a         4495 c         4586 g         2689 t
ORIGIN      
        1 cgcccgcttc ttccctgcgg agcgatgtca cccggttgcg ggtgcgctac cgcgaagcgc
       61 ttcgggcgga ggtgcgccgc acggtcgagc gcgagagcga ggtcgagggc gaattgcgcg
      121 aacttttgcg ggtgttgacc aaccgatgag agggaatcgg aatgcctgca tccaccgttt
      181 ccacgatctg cacgatctgc ggcgagccct tgcaggggag gagcgactgt ctcgtttgcc
      241 tgctgcgcgg cggattggat cggcctaacg agactgcgag ttcgccggct ttcggcgagt
      301 ttgagatcga gcggcgcgcg gatggcacgc tttgggagct gggccacggc gcgatggggg
      361 tgacttatcg ggcgcgcgac caggtgttgc atcgcgacgt cgcactgaag gtgatcgatg
      421 tgccggcggg aacgggggga gcaaaaacgg cgcgcgaacg gttcctgcgc gaagcgcgcg
      481 ccacggcagc tttacggcat cccaatgtcg cgggcgtttt tcagttcggc gactcgtccg
      541 aaattgatcg ctgctattac gcgatggagt tggtcgaggg ggaaactctt gaggcgctcg
      601 tcaggcgaga gggcccggtc tcagcggacg ccgcgctcgc aatcgcgatc caggtcacgc
      661 gcgctctcgt cgccgcggcc gcccacggtt tgattcatcg cgacctgaag ccggccaaca
      721 tcatgctcgt gcctaacgac tcgggtgccg cggcagtgga ggtcaaggtg atcgacttcg
      781 gtctcgccaa ggcaactgcc ggggtcacag aaatggacct gacccacggc gcgttcgtcg
      841 gcacgccgac cttcgccagc ccggagcagt ttgcgggcaa gccggcggac gcgcgctcgg
      901 atatttattc gttaggcatg acgctctggt acgcgctgac cggggaggtc ccgtatccgg
      961 gcaggacgat tgaggaaatt cgtgcctcgc gggcgcaggt ggccttgccc gtcgaacaat
     1021 tggcggcacg gaaaattccc acgccagtga tcaagcttat ccgccgcatc cttgcggccg
     1081 acccgcccgc ccggccgcaa tccgcgcggg cattgttagc cgaactggaa aactgccgga
     1141 cggacatgca gacaacgccg cgtcgccggc ggcggcgcac ggctgctgtt gtcattggcc
     1201 ttttggtcgt tagcctggct ggcctaacga actaccggtg gcgccaaccg cctcctccgg
     1261 ccgccgcgcc gcgcgagaaa tcgatcgcgg tcctgccctt cgaaaatttg agcaaagacg
     1321 aaacgaacgc ctatttcgcg gccgggattc aggacgatgt cctgaccagt ctggccaaga
     1381 ttcatgcctt caaagtaatc agccggacga gcgcgatggc ttatgaaaaa cccgacgggc
     1441 gcaagatgcg cgaaattggc cgggtgttgg gcgtggagaa cgtcctcgag ggcagcgtcc
     1501 ggcgcgaagg caaccgggtt ctgctcaacg tccaattgat cgatgcccgc caggaccgcc
     1561 acatttgggc cgagcgctat gatcggaccg aagccgactc gattggtctg caaggcgaac
     1621 tcgccacaca gattgcggcg gcgctcgagg cgaaactcgc tccggaagaa gtggcgcgcc
     1681 tggcggtcag gccaaccgac aatcccgagg cctacacgct ttatctgcgg gctctcggac
     1741 gagagcgagc ggccaatcgc tcgaccgagg atttggacga tgcggagcac ctttacgagg
     1801 aggcgatcaa actggatccc aggttcgctc tcgcacacgc gcgcctctcg attgtcgaga
     1861 gctccatcgt gggaatggag ggggatccaa tccgaaaagc aaaagcgcgc cgcgaagcgg
     1921 aagaagcgat tcggctggcg ccaagactgg gcgaagctca catggcacta ggcctttcct
     1981 tgtattgggc ggataagaac tatgacgccg cgctcaagga attttccacc gcggcggcga
     2041 ccgcgcctaa cgaggcggat attttcaact tcatcgcggg aatttaccgc cggcagggac
     2101 gttggcggga ctcgctcgcc gcctatcagc gagccttgga tctggatcct cgcgaccgca
     2161 ggatgctctg cttggcggcc atcgattata ccctggttcg ggattggtct gcggcgtccg
     2221 cctgttacca tcgcgctctg gagattgcgc ccgattcggc ccctgcgagg atcggtctcg
     2281 cctggctcga agtgtttcgg aacggcgacc ctgcggctgg gagaaaaatc ctgcaggaac
     2341 ttcctcccgg catcgatccc gatggcgaag tgacggcggc gcgctgggat ctggccatgc
     2401 tggagcggga ttatacttct gccgaaaaga tcctgaccga ttttccctcc gcagaatttc
     2461 cgcacgtcgg tcgagagccg aaaacattct acctcggccg tgtcgctctc gcccgcggcg
     2521 acactgcatc cgcccagcgc aactttgctg ccgccgtccc gggcttcgaa gagtggattc
     2581 gtgagagtcc gaacagtgcc tccccgcacg gtcggttggg actgctctat gcgtaccagg
     2641 gccggaaaga agacgcgctc cgggaaggtc gccgcgcggt ccagctcgag ccggagagca
     2701 aaaatgcgtt ccatggagcg gtcgcggcag ccgagctggc cttgatctat gcccggacgg
     2761 gcgacgcgga tcaggccatc agtttgatcg accgactcct tgcgactccc ggcccggccc
     2821 agcctggatt tccggaaaac atcacgctcg ccgacctccg tctgcgctgg gaatgggatc
     2881 cgttgcggag cgatccgcga ttccagaaaa tcctcgccgg gccggagccg aagacggtct
     2941 atcggtagcg gcggcggctc gttaggccag gcccgaaacg cacgccaggg aacgcatcag
     3001 gcggcgggat ccagcacggt gagaaagggg aagcggtgga agtccatgtc ccgcgtgtag
     3061 atttccttga tcccatgttc gcgcatgagg gtggcggtgt ggagatcgtg caaaatgttg
     3121 ccccgcagtt ggggggctgc gtcgatgatt tcatttaaca aagcggcatg ctgcgtggtg
     3181 ggcagcagca ttccggcgct cggcgaatcg agcaacgtct cgagaaaatg ccacgcggcg
     3241 ccgatcgacc acgggtgcgg cagaatcctg gggtgggtgc aaacgcgcag gaactcataa
     3301 caaatcggcc acgagagata ccacggcgca gggcgcgcgc gacacgcctc gatggtgcgc
     3361 cggcagggtt cgtgaaattc cgagtcccgg ttgacgacat caaccagcac gttcgtatcg
     3421 accaggaaca ttcagcgccg ctcttccatc gcatcgtaga gtgcgttccg gtcggcgatc
     3481 tccaccagag gctttccccc gtgaaagcgc ggcaacggag ccagtttctt tttttgcggc
     3541 agcttgccgc ggtggaagag gagccggagc gccgcctcca ccaactcgga catggtccga
     3601 ttttgccgcg ccgcttcccg gcgcaactgg gccatgaccg tgtcatcaat gttcagggta
     3661 gttttcagat ggcttaccat atcgctgaga tggcaatatg gtcaaacctg aaattgcctc
     3721 gacagccacg ggaaatgacg ctcttttgcg aacgcacgca cgcctgacca tggatcccag
     3781 gaaattcttt gccgagctgc aacggcgcaa tgtctaccgc gccggtgtca tctacgcgat
     3841 gtcggcctgg ctgctcatcc aggtggcgac ccaggttttc ccctttttcg agattccgaa
     3901 ctgggtcgtt cggtcgatcg ttgtcgtcct cgtcctcggt ttcccggtct ctctcgcgct
     3961 cgcctgggcc ttcgagctga cgcccgaagg gctcgtcaaa accgaggatg tccccctggg
     4021 aaaatcaatc cgctggaaga ccggccggaa actcgacttc gtcatcatcg gcatcctcac
     4081 ggtggcgatc ttgtttctcc tttttctcca tttctcgccc tcccaccggg cgacgcgcga
     4141 tcccgctatt ccggagaaga gcatcgccgt tttgcccttc gaaaattaca gtgcggaaaa
     4201 agagaacgcg ttcttcgccg atggcatcca ggacgatctg ctgaccaacc tggcgaaaat
     4261 caaggcgctg accgtgatca gccgcaccag tgcgatgaaa tatcgcgacg tcagcgcgcg
     4321 cgacatcaga gagatcggca aatcgttagg cgtggccaac gtcctcgagg gcagcgtccg
     4381 gcgggagggc agccgggtgg cggtcaacgt gcaattgatt gacgccttca acgatcgcca
     4441 catctgggcc catcgctacg accgcacgat cgcggattcg ttaggcttgg aaggcgagct
     4501 ggcgacggaa atcgccgacg ccctgagggc ccagctgacg ccggatgaaa aggtgcgagt
     4561 ggccaagaaa ccgaccggga acgcggatgc ctatgtgctt tacctccggg cgctccccta
     4621 cgagcagggc ccggacaccc tgctccagga ttaccggcaa gcggtcgagc tatataccaa
     4681 ggcgatcgcg ctcgacccgg agttcgcgct ggctcgcgcc catctcgcct ccacttgcgc
     4741 ggagattttc catttccatg agccgctcga ctcgtggaaa gcgcgcgccc ggagcgaggc
     4801 cgaagccgcc ctccgcctgc aacccgatct gggcgaagcg cacttcgcgc tcggccaatg
     4861 cgcctactgg ttcgacgccg attacgaacg cgccctcgcc gaatttgcca tcgcgcaacg
     4921 cgccttgcct aacgacagca acatcggcgc ctatgtcgcg gcgatccgcc gccggcaggg
     4981 gcgctgggag gaggcgctcg ccacctatga acggatggaa aagctcgacc cgcagaatcc
     5041 gaacatcatc cgcaacgttc tcttcaccaa cacctcgatg cgtcgctggc cgcaagcggc
     5101 cgaggcggcg gcgcgtttcc gggcggtggc gcccgactcg gtggtcgcga aaattcaatc
     5161 cggctatgtc gagttcatgt ggaaaggcga catcggcgcg ctgcaacgct ggctcggtga
     5221 cattcctccg acggacgatc cggatgggat tgtgacctgc gtgcgctggg aaggggcgat
     5281 gttacaccgc gacttcgtcg gcgcggccga cattctggcg aagtcgccga cgagagagct
     5341 ttcctatctc agcggcggat tgcagccgca gagtttcctg cttgggatca cggcggtggc
     5401 cagcaacgat cgggcgggcg ccgcgagaaa tttcgccgcc gcccagccgg aggtcgagaa
     5461 ggcggtgcag gaagcggccg acgcgcccgc ctgccacgcg aaccttgggt tactctatgc
     5521 cttcatgggc aagcacgacg aggcgattcg cgaaggccgg cttgccgtgg aactcaaacc
     5581 gatagcaaaa gacgctaacg atggtgcgat cgtgctttgt tacctcgcct tgatctacgg
     5641 gcgggtcggg gaaaaagatc aggcgattcc gttgatcgac cggttgctca agacgccggg
     5701 cgcggtcgac agcgtcaact acagcatcac cttgaatgat ctgaagtatc gctgggaatg
     5761 ggacccgctg cggggcgatc cgcggttcca aaaacttgtc ggacaaaccg cgccgccgcc
     5821 aaaatgagtt gtcaagcagg cgctccgccg gccggcgcta ggcaggataa tgccacgcgg
     5881 ccaactcagc gcggagttct tcgctgaaac gtctctgcaa atcggccgga agtttttcgc
     5941 gccattgccc gccctttccc acccgcagaa attttgcccg gcccgtttcg ccgctttgca
     6001 ggagcgtttc tttcttgcgt gcaaacgatt ggttgtggac cgcggtcgcg atctcttcgc
     6061 tcgtccgctc gatcccgaga tgatgcagga tgcgacgcgc ttcttccccc gggttcgcga
     6121 gcaggtcttc gtaccggaca atcgggaccc ctgcttcctg atacggttgc caatgttccc
     6181 gccacgaaac ccggcaccag ttgtggacct gggcgcagcc ctcgagcaat gcggtcgtca
     6241 ttcttcccaa ccgatagtcc tggctggcca ctagcggata aagcgtgcgc tgatagattt
     6301 ttttcagccg ccccccatgg aaaaggcggg ccagcctggg aaaaggatcg aaggtaaaat
     6361 gatgcgcccc ggagatcgcg acgtcgcgcg gatcccgcag cacatagatc acgttttttt
     6421 tgacctcgtc cacgggcagg agcagctccg cgagctgatg atgcgatttg tagcagcgaa
     6481 aatctgaaac ccgattttcg ccctcgatcg cgatttcttt cttctccagg cgccaaaagc
     6541 cggccaccgg gcaacccacc agctccgcca ccaaccgcgt tgcccaggtg catccgctct
     6601 tcgggtagcc gaccacgaca atgtttttca tgcagacgga ccgcggccta acgagtctcg
     6661 cgaaggtcgg aaaacgccgc cccgaggtgg tcgagcacgt cgccgggcag gagcgattct
     6721 tcgctcgcat gacgattgaa gacagcgagc tccgccgcgc gcccgcagag ccaggcgcca
     6781 agccgggcgg catcgcgcgg cgccagcttc gcgccgagga gcgccgcgca caccccggtg
     6841 aggacatcgc ccatcccgcc cgtcgccatc ccgggattgc cggtcgtgtt gtaactcagc
     6901 ggccgcgcgg cctcggcaac aattgtgcgg ctgcctttta ggagcagggt cacggcgtgt
     6961 tgcgcgcaaa aattcgtcgc gatttccgcg cgcgacattt tttcggtcgg aaaaagccgc
     7021 tgcatttcac ccggatgcgg ggtgagcagg cgcgcgcccg gcagttcgtc gagcatcgcg
     7081 cggcgctccg ccaggatatt gagaccatcg gcatcgacca ccatcggttg ctgcgccgca
     7141 cggatgagcg cgagaatttc cgccgctcgt gctttgccca ggcccgggcc gaccgcccag
     7201 acatcgatcg gctcttccaa gagcgcggcg taggatttga ccggcttgac catcacctcg
     7261 ggagtcgaag ccgtggctac gatctcgtag atttcttccg gcacaaagag ctccaccaag
     7321 ccggctccgg cgcgcagtgc gcctaacgag cacatgaccg ccgcgccggt gaagccgcgc
     7381 gagcccgcca cgatcccgat gcggccgaac tggtttttgt aggccgaata tctgcgccgc
     7441 ggcaaaaggt cgcgcagcac ttcggcgatt gccgcctcgc ccgcgcccgg gtcctccgcc
     7501 cgcagttcgt cgagcagaac gacttccagc cggccgacaa aatcaatcgc ggaatccgcg
     7561 accaaaccgc gcttcgccgc gccgaccgtg attgtgaaat cggcgcggac acactcggag
     7621 tcggcggcgc ccgagtcgcc gtccaggccg gtcggcaggt cgatcgcgac gacataggcg
     7681 ccggcgcgca gcgaattgat ttcgcgacag gcgctccgga tgggctcgcg cagcggcggg
     7741 tgcgcgccga ggccgagcag cccgtcgagg atgatggtct gggagtgtgg gcgcggcctg
     7801 ggtgccccga tccccggggt cacgcctttc ggggcgccaa gatccctccc acattcctga
     7861 tcgcggagcg cgcccaactt ctcactcgtt agggggctca actccttctc cgaaaaggtc
     7921 agacgcgttt cgattttcca acccgcttcc gccagccagc gcgcggcgac caaagcgtcg
     7981 cccgcgttgt gtcccttgcc cgcgaagaca atgcactttc cggggcgcgg gaaaaatctg
     8041 gtgagcgcgc ccgcgatccg ttcgcccgcc tggtccatca gcgcctccgc cgtgattccg
     8101 cgggcaaagg ccgcttcctc ggccgcgcgc atctgcgcgg gagtgatgat gggcgagtgc
     8161 aatttattcg tcggattccg gggttttctt ctttttcttt ttcgtcgtgc tggtggaaga
     8221 ttttttgtgg cggctgctct tcttcgagga tttgctcgaa tgatggctgg agcccgtccg
     8281 ttccggctct gcccggacaa aatcgttggt gttaaacggt tgctcgacgg gcgggacgac
     8341 atgggtcaga ccggccacca ccgctcgatt gggcggcgcc cgtttgttca tgaccccgtt
     8401 cgcaatccgt tgggcgagct gctcgcgata actgctttgc aacgcgagcc gcgcttcggt
     8461 cgggttcgtg aggaatccac attccacgag caccgccggg atgctggtgc ggcgtaggac
     8521 aaaatagccg cgccggcgga tgccgcgatt ttccgaagtc gtgcctgcga ccacctgccg
     8581 gtgaatgttc gcggccagcg ccgcgctctg gttgctgtag tagtaggtct caatcccgtt
     8641 cgcgcccgcg cgcgtggcgg cgttgaaatg aatgcagacg aaaatggcgt tgccataact
     8701 gttggcgatg cgcacccggt cgccgagggt gacgaagacg tcgctgtcgc gggtcatgac
     8761 cactcgataa ccggcgtcgt gcaggattct gcgcaagcgc agggccacgt cgagggtcat
     8821 gcttttctcc gccaccgcct ggccggggat cccgccgcga tcgaacccgc catgaccggc
     8881 gtcgatcacc acggtgggaa ggttgctgct cgcggaccag gccggtgcgg gcaggagcga
     8941 aagagcgatc gcgaccaggg cgaagagtgg tgcgcgcttt ggtgcgcgta atcgtttctc
     9001 cgccatcgcc attaattttc cagcggcaga tcagccggcc gatcggataa ctttggcccc
     9061 tcttttccgg aggctagcgg cgcgactggc gcgtcgacca ttcctccggc gaccgccacg
     9121 gtgccgtcga ccgcgcggcg gagatgcggc gaagtgcgtg tctgggtgta ggtggtgtgt
     9181 ttcgccaggc gtccatcgag gccgacgacg gaataggacc agagccgggg agcggagcat
     9241 tgcaggacgt gcaactgatt attgcgatcg acttcggcgt gcggttcgtc gaaggaaatg
     9301 agccggccta acgagtaggt cgcgtagacc aggctgttcg ccttgtcctc gacccgcacg
     9361 tagagagagg tgtgatcggg aaaacgattc gtcagcagcg aataggtgcg gacgccgtcg
     9421 gctcccgggt tgcccaccgt ccgctgccag atcgggcgcg cgtttgtcac ctcgaaaacc
     9481 ttggcgcgcg cgtaaaagaa cttgttcaaa tcggcgaagt aaacattcgc ccgcgcatgg
     9541 tagacaccgc ggtcggtgat cgggaagagt ggagtcagat cgatcttgcg agtgacggaa
     9601 gtgccggccg cgattttcaa aggcggctcc gagcgctggc ggagcggggc gagggtgcgg
     9661 ccttcgttcg cggtcacttc gaagccgtac caatgctggc cggtatcgtc ccgcaactcg
     9721 atctcgcgcc cggccaggtt ggtgatcgtg acggttgcga tcaccggttc ataggcgatg
     9781 tattggagtc tcttgaattt caagtccacc tcgatctggg cacgcgccgt gatggtcgcc
     9841 aaaaaaagca cagcgagtgc cagaagccgg gtcataggcg cgtaaaatac cgcaactccc
     9901 cggagatgaa aggcaggcgg ccgaaatcag ccgcgttttc cccaaagctt gatcttgtcg
     9961 agcacgccgg ttttgcagcg cgaaagcgcg cctgattccg tgctcgtcga gacgggcgag
    10021 cgtttcggcg atcgcgcgta cagcgcggag ggcgaagtct gcccgatctc cgccccgtga
    10081 tgaacgatca acaattgcgt cactgcggcg gggcggtgac aggcggcggg aaaagattga
    10141 gcaaccgcgc cggctcggcg cggaccgctt gcaattggtc ctggtaataa acccgcacga
    10201 tatagccgag gtaggcccgc gggccgctct cgctcggcgg gaggagcgcg gggggtgatg
    10261 gtttgcgcgc ggccagcttc gccttctcca ccgaatcggg cacttccacc ggctgattcg
    10321 ccagagcggt gcccggcgcc ggctcgttgc gcttggggcg caggtaggtg acatcgagca
    10381 cctctgtccc ggtatcggcc caatcatgcc caggcgtgac ccattcatag ctgacgtccg
    10441 cgttggtcag gacgacctga tcgttgttca ccgtgtcgta gaagaaaacc tggattttta
    10501 ccttggtgtg atcgatcggc gtgtcggggc gcgccttgac tccgacgcgc agcaaaagct
    10561 tcgtctcggc atcgggctcg ggctcggttt tcagtgtcac ctcggagatg ccgaaggtcg
    10621 atccgtcggg aatccctgcg gcgtcgctgg ggggcgcgcc aggcggggcg gtcgatccgt
    10681 tggccctgag cgcattgctc tgcgcgggca cgcccacccg cagcttgacc tcggccagtt
    10741 catagagcgg accaaccgcc gggcctaacg attggagacg cctccagacc tcgttcgagc
    10801 gatcgaagag ctgcatcgat tcgtaggtca acgccagttc cgccagaatc tcagggttcc
    10861 cggggtcgag tgcggtagcc tgctgcaact tcgcgatcgc gttggtcgta tcgccgcggt
    10921 cgcggaaacc ccttgccacg cgcaaaagcg tttcggctac cgaggacggc ggagcctgcc
    10981 ctcgccggac ggtcgcctgc ggcacgccct ctaccgcggg cacagtcacg ggcgcggcgg
    11041 aaggcgcggg caacgattgt ttgaagaggc gggcgaccgc cgcgttttgc gacgcaaccg
    11101 atggcgcctc gcccggatgc cgggtcgaga ggtaaagtgc gctgaggatc tgcgccacgg
    11161 caactgccac caggagcgcc atcgcggcga cgaaggtttg ctgcgatcga ctgccttgga
    11221 gaaaagagac cgcgcgcatc gggaacgttc tctaactgaa tgcgccgcaa ggtgtcaatg
    11281 agcgaagccg ctcctgcaca ctcgtcctcg tggtcttacg cgaacggttt gggatcgatc
    11341 acgatcacga gcacgaggac gaggacgagc gggagacctt caacgggaga tatccgcctg
    11401 gctgagcagc ttgaagcctt caccctggag caccgagcaa gcgcactccg tgtcgtctac
    11461 gtggaggacg agcgcggcgc gcccgtgcgg gcgggtgaga aggggatagc tgaaatgcat
    11521 gttcacctcc gccatgagga gcgcggcgag tagcttggcc acctcggtcg ctacctctcg
    11581 caactccacc accaccagcg cgcagacccc aaacgggaca ttgtgttcga gaaaaatatt
    11641 ctccgccgtc tccggatcgc tcaccacgat gcgtgcgatc gccgagtcgg tcgactccgc
    11701 gatgctgagc gcgaccaaat gggtattgcg ggcgctgagc aacttcacaa tgtcgagcag
    11761 tgctcccacc ttgttcggca ggaaaacaga gaattgcctg accagcggac cgtcaatctt
    11821 ggaagtggtt tcaggtaaac tttgggccat aaaaatcgcg atcaagcgag ccgacactat
    11881 gccgccgcgt gggaacgcaa cgcaaattaa tagtgcaggc tggagcggca gccgtgtcgg
    11941 gcgcagtccc cacaagagcg gctcgacacc gaggcaccgc ccggacatta atatgaacaa
    12001 ccgattgaaa ccgaaacttc acacaaaaga aaggagccag gcatgaagca attaccaaaa
    12061 gcaaagatcg acgagttcaa agcacacttt cgcggagagg tgcttcttcc gggagacgtg
    12121 aactacaatg aggtgcgaca aatctggaac gccatgatcg atcgcagacc cgcgctgatc
    12181 gcccggtgca cctcgccaca ggatgtcgtg cactcggtca agttcgcgcg tgagcaggat
    12241 ctgctcgttt ccatccgcgg cggcggacac aacatcgcgg gcaatgccgt ctgcaacgac
    12301 ggtctcatga tcgacctctc gctcatgaag ggcgtccagg tcgacccgaa cgcccgtcgc
    12361 gcgaccgtcg agcccggatg taccctcgcc gacttcgacg cggtcgctca gactcacggc
    12421 ctggcgacgc ctttgggcat caactcgacc acgggcgtag ccggcctgac gctcggtggc
    12481 ggctttggct ggctgagccg gagatacggc atgacgatcg acaacctcct ttccgcgaat
    12541 gtcgtcatgg cggatggcag ccaggtgcga gccagcgaaa cggaaaatgc cgagcttttc
    12601 tggggactac gtggcggcgg tgggaatttc ggcatcgtca ccagcttcga gtttcggctc
    12661 catcccgtcg ggccgaacgt gctgagcggg ctcatcgtct tcccgttcga tcaggcgaaa
    12721 tccgtgatca cgcaatttgc ccgtttcacc gagacgatgc cggaagagct gaacgtctgg
    12781 atggtcactc gcaaggcgcc tcctctgcct ttcctgccag aggccgtcca tgggaaagaa
    12841 atcgtcgcgc tcgccttgtg ctatgcgggt gatcccaggg aaggcgaaaa gctgatcgcg
    12901 ccgttgcgag ggttcggcac ggcgcacggc gagcacgttg gcgtgcagcc gtatgtcgcc
    12961 tggcagcagg ccttcgatcc gcttctcgcc aagggtgccc gcaactactg gaagtcgcac
    13021 aacttttcgc ggctgagcga cggcgcgatc gacgccatca tcgaatacgc cgggcgactg
    13081 ccgtcacccc attgcgagat cttcatcggc acgatcggcg gccagacaac tcgcgttgcg
    13141 cccgatgcga tggcctactc gagccgggac gcgaactacg tcatgaatgt gcacggccgc
    13201 tgggagtcgg ccgccgagga cgagggcgtt ataacctggg cgcgtgaatt tttcgccaag
    13261 tcaaagccat tcgccagcgg cggcgcctac atcaacttcc tcaccgaaga agagaccgac
    13321 cgcatcgagt ccgcctacgg tccggtctac aaccggctgg tcgcgctcaa gaagaaacac
    13381 gatccgacga atttcttccg gatgaatcag aacatcaaac cggcctgaaa gaacgtcgtt
    13441 ccgcctaacg atggcatcga ctcattaaga tgggacgcgg accgatcact ggcggctgtt
    13501 actgtggtca ggtgcgctat cgcgcgaagc aggagccgat ttcccacgcc aactgccact
    13561 gtgccaattg ccggcgcgcc gccggcgccc aatcggtcgc gtggattacg gtcgcgcact
    13621 ccgcttttgc gttcgagaag ggaagcccac gacggtatcg cgccgatacg ggagcgtgga
    13681 ggacgttctg tgaccaatgc ggaacctcgc tcacctacga gagcgacacg cggccgggcg
    13741 aaatcgacct gacgaccggc agcctcgacc atccggagga ttttccgccg aacaaggacg
    13801 tctttcccga ggagaaactg ccctgggtta gtttggtcga tgaaacaaga tgctagccgg
    13861 ccggcgcaat tgatgacccg ccgccaaatt tcctccggag gcctcgccat gattgcgatc
    13921 aagtgaggcg gagcgaattt ccagccgcga tttcggcttt ttccgcccgg tcgcaaatca
    13981 caatcatcgc cagactcgcc tcgtgcccgt ccaacccgct tttgccagcc aaccaacccg
    14041 agatatgcga aaaaatgttc gcctttaaag gcgaacgttc ttctatatct tgttaggtct
    14101 atgacgcacc gccagcactt cgctatcgcc ggaatctttt tgcttctcgc gaccaaagct
    14161 ggcgcctttg acttcgagtt cgcacgggct acctcgtcgg caggacaatt ttctgtagtc
    14221 cttccgaagc cattttccga gttaccggcg aatgccgggg taaattcagc cgccgcgatt
    14281 aaaccgaagc aatcatttgt catcggcggc aagcccgcac ctagagtcgt ctttattgct
    14341 acgaagatgg tctacggtag cacaagcgat gcacgatcgg tcgtgacgag catcagtggc
    14401 ggtgagccgc caggattcag gcgagcctac ataaaaaagg ttcaggtggc gggattgccg
    14461 ggtttagaaa tcaagagcct ttcgccgtca accgtgggat atcggcgcgt ccttcttgcc
    14521 ggcgacacg
//
LOCUS       DATMNS010000206        17191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2434397, whole genome shotgun sequence.
ACCESSION   DATMNS010000206 DATMNS010000000
VERSION     DATMNS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 17191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17191
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434397"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="VNW28_07000"
     CDS             complement(<1..914)
                     /locus_tag="VNW28_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HXA09713.1"
                     /translation="MGNNPELAPQPKSLADILAEELDGQLGTDETQELPAAEKQPRLR
                     AVYNRIHAEQPERSALCLSGGGIRSAIFSLGVLQGLASNNLLGQFDFLSTVSGGGYVG
                     GWLTAWIKNHPQGAAGVVEDLRRRPDLTLNPEPQPIRHLRAFSNYLTPKTGFTSVDSW
                     TLIATYIRNVFLNWLVLISWLAAAMMVPRLYLAAILLPPTGWASSPNYTSIMQQYDIA
                     LTALLSTCFVLVAVAMAYAIIDVPTTGNAGFPQRRFLEVRQIPLFIAALGLTEWWALF
                     CNIHGSQAFHSSAGLLRFVAFAIATYLAG"
     gene            1470..3263
                     /locus_tag="VNW28_07005"
     CDS             1470..3263
                     /locus_tag="VNW28_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09714.1"
                     /translation="MHPKHFFAELKRRNVYQIAVAYAVIAWLLIQVATQVFPFFDIPN
                     WIVRVVVLLLIVGFPIALVWAWAFERTPGGIKHERDVPRNESITRRSGRKLTALIVVV
                     AAAATGVMAVRFLRSQEESENAQPKAATENKSLAVLPFENLSADQSNAYFVSGMRDEI
                     LTSLAELRDLNVRSRTSTQRYPSHPENLEAVGRELEVATVLEGSVQKVADDVLINIQL
                     IDTRSRKQLWGQSYRRKTGDIFSVQGEVAEQVAEALKIRLAPAQEQRLLTPPTTSARA
                     HDLLLRAHALGAHSDEQSLEGKIAFLQEAVADDPHYALAWGDLAGAYLTIADAYRAPL
                     DVLGPMRHAALMAVKNDDGAGVGHIWLGAVSMLYDRNFPLAKSELERAVALDPNSSDA
                     HRWLGWYLARVERDFDAGRAELQRARALDPSYTWPLMFECYVAIARGDYASALQLAQQ
                     VMEIDPHFFYDVDPIASVYAAEGRWPDAIKRYESLPAGTITRPNFQLAICYARTGQSD
                     RARHILADLETLAQQRYVDRSHLAGIYAALGDKDKAFAALDQACADRSARVSTPRFYT
                     WLAPLFDDPRFAALEDKVAHSALASPLEVQP"
     gene            3395..4504
                     /locus_tag="VNW28_07010"
     CDS             3395..4504
                     /locus_tag="VNW28_07010"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09715.1"
                     /translation="MVAGDSFCNRAREKADLAQAIRNHEKLFVFSERRFGKTSLVRAV
                     LAGFSKRTTVCAYVDLWPTDNEATFVTAMAKAITQAMSSSVGKLLETGKSLFSNLAPS
                     VTLDEGGKPEVNFGVSRHTRIVPALDEVLETPAKIAAKHDRDVVIVLDEFQQVLEYGS
                     DRVEKKFRSVIQNHREVAYLFLGSRKHLIQKMFMDRSRPLYRAGGHYPLGPIAEEHWM
                     PFIRERFAGAGKKISQGLIHSICEQTQGHPFYTQHLSHALWELCEPGATVTEGLLKEA
                     LRLLLQRENYAYSTLWESLAMNQRKFLKGIASEAAPVKPFAGEFVRRSGLGSPSNAQR
                     AVDALLDRDVIDRENGSFLITDRFFRLWVQRVQLP"
     gene            4620..6665
                     /locus_tag="VNW28_07015"
     CDS             4620..6665
                     /locus_tag="VNW28_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09716.1"
                     /translation="MNSGNFFSELRRRNVYKVAVAYAVVGWLLIQVATQVFPFFEIPN
                     WVVRLVVLLIVLGFPIALVIAWAFELTPQGFQRTEIADKLPNHSARSRAWIYVVIVAG
                     LFSAGLFFLGRFAAAPAQSGAAEAPEKSIAVLPFENLSSDKENSYFTDGIQDEILTRL
                     SKIADLKVISRTSTQLYKSAPKNLPEIAKQLGVAHILEGSVQKSGDQVHINVQLIKAA
                     EDSHLWADSFDRKLTDIFSVETEVAKTIADQLQAKLTGQEEQVIAARPTENPEAYDAY
                     LRGLAYNLKTANSAANSRGAQKYLREAVQLDPKFALAWTLLSFVDARGYLTQTLQPTD
                     ALREEARQAATTALTLQPNLGEALHAKGYYHYACLKDYTTAVRYFEQARPFLPNSSRI
                     PESLAYVTRRQGQWDKSESYFNEAERLDPRNVYVLGQHGQSYIALRRFSAALRKFDQI
                     LTITPDDMDTIAVEASIAQAEGDLRRAAALLAPLHPPADDSNVLFTQVYQAILERRPA
                     PAIPHLTSLLAKPDPAWGYFTGELRFWLGWVQDVAGDHAAAQGSWRQARGELEPFLRE
                     QPDNYNLLFDLALTDMGLGDKAVALAMSERAMAAMPIEKDAFDGPAPIEILARVAAQV
                     GEPDRAIAALQKVLALPYEGPLGGGQAPLTPALLRLDPMFDPLRNDLRFQQLAAGQR"
     gene            6662..8533
                     /locus_tag="VNW28_07020"
     CDS             6662..8533
                     /locus_tag="VNW28_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA09717.1"
                     /translation="MNFFSELKRRNVYKVAIAYAVVGWLLIQIATQVFPFFEIPNWVV
                     RLVVLLIIVGFPIALIIAWAFELTPGGIMRTEAADRVPAAPRKTHTWLYLVVICGAFS
                     AALFFLGRYTASNHENTSGRLAKSIAVLPFENLSPDKDNAYLAEGIQDEILTRLAKIG
                     QLKVISRTSTAKYQSGPDNLREIAQQLGVGNILEGSVQKARDQVRVNVQLIDATNDAH
                     LWADSFDRRLTDIFALESEIAKAVADSLQAKLTGSEAHALAVHPTEDPEAHRLYLLGR
                     FFWNKRTGPDFRKAIGYFQQAIDKDPGYALAYAGLADAYVLLSGYSAATPKESLPEAK
                     AAAQKALELDESLGEAHAALAEAIFAYDFDVAGASREFRRALELNPNYATAHQWYAES
                     ALASQGRFDEAVAEMRRALELDPLSVVINADVGTILCTARRYDEAIEQLRKTVEMDPA
                     FYYAHWNLGQALELKGQLKEARAEYEKAITLSDDPLPRAFLAELCAKTGQKEKAREIL
                     AELRRVPKDTYVSPYNFALIYIGLGEKDEALRELENTYEDRDGYSIVFIKVDPMLDPL
                     RGDPRFEALVEKTGRPFAAAQSGTNGRIDRATFALCRVELTGANPFSDVSRGPGGKQ"
     gene            8665..8883
                     /locus_tag="VNW28_07025"
     CDS             8665..8883
                     /locus_tag="VNW28_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09718.1"
                     /translation="MDAFDLIAPRRQLAGSLIGGIAETQEMLDFCAELGIACEIELIP
                     IDQINEAYERMLKREVKYRFVLDMASLR"
     gene            8931..9440
                     /locus_tag="VNW28_07030"
     CDS             8931..9440
                     /locus_tag="VNW28_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09719.1"
                     /translation="MNWDAINAISQLVSSVAVVLSVLYLAKEVHRSTRVAKVAAQDSA
                     ASAVRDVTNTFMENADMSRIWGTGLEDIKTLSREDQARFFHAAHQFLKALETIHFHYV
                     NGLMDEQLWRGWQELLRHYIVAPGIARYWEIRDQLFSARFREFVSQLEPLRDRRTVGT
                     LLEQEDLPE"
     gene            complement(9711..11999)
                     /locus_tag="VNW28_07035"
     CDS             complement(9711..11999)
                     /locus_tag="VNW28_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09720.1"
                     /translation="MRGALARILALLAVLLLATAAFQVTSLPGSAGAERRRSEINGET
                     RASKESPPEDWFVTQRVTHGGIPAGALEKAGAQAAALAVASEQSGPQTRSRWRFVGPT
                     NIGGRVVDIAVDPVAADTIYVAAATGGVWKSTDRGANFTAIWPATNPQSMGALLITSN
                     GTLFAGTGEANPGGGSITYGGSGIYRSVDGGATWLLVGLTSSGAIGRLAVDPTNSQNI
                     FAAVAGQLYNHGGERGVYKSTDGGSTWTQVLAGDNDTTGAVDLAIDPTNPNRVFAAMW
                     DHFREPDLRTYGGVGSGVYRSIDGGTTWQRLTNGLPAAGTTIGRIGVALAVSNPQRLY
                     AIVNQTNGLFQGFYTSADGGNTWTKLPTSSTLSGAQSTYGWWFGRVWVDPLDQTHVFG
                     AGVYLCESKNSGSTFTGEFSPHADDHAMVWDLKVPGRVYLGNDGGTYRSDVNGSNDQW
                     TFAVSQPFTQFYSVDVSEQDSTRLVGGAQDNGVNRSYDGTPWNTYIGGDGEEALIDPS
                     DQNMVYGCSQYGDCLRSTNGGDSSTDFTSATVSSRRNWLTPLQFDPANPAVLYYAGNQ
                     VNRSSDHAVHWSVISPDLTGGPGRDPNYPFGTVTTVAGAKTDSNRVLAGTDDGRLWFT
                     TNLGTNWTQVTDSDVPGTWVTRVMVDPVNAQVAYATFSGFRSGVELPYVLKTADGGTT
                     WSSIVGNLPQAPVNDIVVVGSTLYVASDVGVYSSANGGRKWRAAATGLPNVPVTDLEY
                     RAASNALYAATFGRGMFMLALP"
     gene            complement(12141..13163)
                     /locus_tag="VNW28_07040"
     CDS             complement(12141..13163)
                     /locus_tag="VNW28_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HXA09721.1"
                     /translation="MAKKMRAVQVPEAKGPFEIVEMEIPEPPAGWVRVKVQACGICHS
                     DTLVKDGLWPGIQYPRVPGHEVIGTVDAVGSGVEGWKSGQRIGVGWHGGHCGYCDSCR
                     RGDFFACQIALLTTGISFDGGYAEYLIAPAKALASMPNELSGVESAPLMCAGVTTFNA
                     LRNSAARAGDLVAVLGIGGLGHLGVQYAAKMGFRTVAIARGKDKEPLARELGAAQYID
                     SQTQNPAAELQKLGGAKVVIATVTNADAMAATIGGLAPNGVFMVIGAAGPIQVDPLLL
                     ISGARSVKGWYSGTSIDSQDTLRFSALAGVKSRNEIYSLSQVGEAYDRMMSGKARFRV
                     VLDMAQ"
     gene            complement(13475..14329)
                     /locus_tag="VNW28_07045"
     CDS             complement(13475..14329)
                     /locus_tag="VNW28_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXA09722.1"
                     /translation="MPRRFLPPVLLLLAALCWSLGGVLIKSIDWPPMAIAGGRSAIAI
                     PVILLCIGRPRFTCSRAQIGGALGYAGTVILFVFATRMTTAANAIFLQYTAPIYVALI
                     GRWYLDERASRVDWLVIAVALLGIALFFFDRLTTAGMWGNIIALASGLAFASVALFLR
                     KEKAGSPIASIVLGNVIVALAGAPFMFQAPSLGEGSLWRLALLGTVQLGLPYVFYAAA
                     IKQVTALEATLIPLLEPVLNPLWVMLALGEKPGPWAIVGGLLVLVAVLGRGVIMMRTR
                     PPMIPSPD"
     gene            14381..15145
                     /gene="surE"
                     /locus_tag="VNW28_07050"
     CDS             14381..15145
                     /gene="surE"
                     /locus_tag="VNW28_07050"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXA09723.1"
                     /translation="MKRADLRELGGQDGAPYTARVTCAPMKFLVTNDDGIDAAGLTAL
                     VGAAETLGEPVVVAPAGPQSGVSHAVTWREGVRIEPRGENRFAIHGTPADCTRLGLLH
                     IVPDAKWILSGINHGANLGADVYYSGTVAAVREALLHGWPGIAFSQYRKSGVDCDWER
                     ATRWITPVLADLLAKPIEPGVFYNVNLPLLAPDAPDPEVVWCPLDPKPLPLNYRHEAE
                     SGLYYAGDYNLRDRTLGADVDVCFGGNIAVTAVRLL"
     gene            complement(15194..15358)
                     /locus_tag="VNW28_07055"
     CDS             complement(15194..15358)
                     /locus_tag="VNW28_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09724.1"
                     /translation="MDNEPNEEISNHASQQVTEPIKEPPLLKLRDLRPEKDPIGAGRR
                     RFPNAGAKRP"
     gene            complement(15388..16254)
                     /locus_tag="VNW28_07060"
     CDS             complement(15388..16254)
                     /locus_tag="VNW28_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09725.1"
                     /translation="MVVLLFLSLLAPADARTQRHRKPRPVAVTVALQPFLVARSVVHT
                     VADPLVRNAPRAIAVVATAPIRVAYRSERNREVYRGEMVEEDEAIARPAVPLAQSAGY
                     DQPLRVAYMVPRSQNPGNPEPPQDDQDLDQEQGAKPAPAKSVHGPTVAGSRAILRGGI
                     ASAPSRAPQSVKNAIWAANALHRKPYIWGGGHGSFYDRGYDCSGTVSFALHGAGAIAV
                     PLPSNDFMRYGERGRGRWFTIYARPGHTFAVIAGLRLDTTDFQDGGTTGPRWHADLRD
                     TRGYVARHPAGM"
     gene            complement(16515..>17191)
                     /locus_tag="VNW28_07065"
     CDS             complement(16515..>17191)
                     /locus_tag="VNW28_07065"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HXA09726.1"
                     /translation="VDPDNAIIAVDNSTQLPFMSNYTGLVMGTNSQGQTILYACDAGP
                     NNRVDMYDEQFNYLASFTDPNVAGQYPDYTASQVENVDGQLFVTFNVTTGAPIGGVVD
                     IFDTDGNLLTPNHFAANAGGAGPLVNPWGVAKAPAHFGQFSHAILIGNIDDGKINAFD
                     SSGHFLGAMQDRDGNDIVIPGLWDLIFTQSDGGGGSVELYFDAGPDLANFAGNGLVGV
                     ITQGKR"
BASE COUNT         3304 a         5345 c         5248 g         3294 t
ORIGIN      
        1 cccgcgaggt aagtcgcgat cgcaaaggcg acaaacctga gcaagcccgc gctggaatgg
       61 aacgcctggc ttccgtggat gttgcaaaac agcgcccacc attccgttag cccaagagcc
      121 gcgatgaaca agggaatctg ccggacctcg agaaaacgcc gctggggaaa tccggcattg
      181 ccggtcgtag gaacgtcaat gatcgcgtaa gccatcgcca ccgccacgag cacgaagcac
      241 gtgctcagca gcgcggtgag cgcgatatcg tattgctgca tgatactcgt gtaattgggc
      301 gacgacgccc aacccgtggg cggcaagagg atggcggcca gatagagccg cggcaccatc
      361 atggccgcgg ccaaccagga gatcagcacc agccagttca ggaacacgtt gcggatgtag
      421 gtcgcgatca gagtccagga gtcgaccgag gtgaaaccgg tcttcggtgt caggtaatta
      481 ctgaaggccc gcaggtggcg gatcggttgc ggctccgggt tcagagtaag atccggccgg
      541 cgtcgtaaat cctcgacgac gccggctgct ccctgaggat gattcttgat ccaggccgtc
      601 agccagccgc cgacatagcc accgcccgag acggtcgaga gaaaatcaaa ttgtccgagc
      661 aggttgttgc tcgccaaacc ctgcaggacg ccaaggctaa aaatcgcgct gcgaatcccg
      721 ccgccggaaa gacaaagcgc cgagcgctcc ggttgttcgg cgtgaatccg gttgtagacg
      781 gcgcgcaggc gcggttgctt ttcggcggcc ggcaactcct gtgtttcatc cgtgccgagc
      841 tgaccatcca gctcctcggc taggatatcc gcgagggact ttggttgcgg cgcgagttcc
      901 gggttattcc ccatgggcga tacctccctg cggccggttt aggctgccgc ggggaaagag
      961 acaagccaat ttcttttctc cgcgccgctg cgatcgagtc tcgcgcggga aaatcggcta
     1021 tgcattgggc taccatgaaa ccgctcccgg gtacctgacg cccgatggag gtttattcgg
     1081 ctccgcatgc ctaacgaacg ggacctcaaa gccgagattg gccacgtgct cttccacgac
     1141 atcgtgtgcg actcgaaatt gctcatcaac gaacagagca agctactgaa catttgaagg
     1201 gaatgggttg cgctcgtgct ggctatgcct ctggccgttt ggtttgggcg cagagaggcg
     1261 gagaggaccg gcccgatcgt gcctaacgac caacgtccgg tcgccttggc gagtttgtta
     1321 gacctcggat tcgcgaaaaa caattcccaa aggattacgg caaagaggac gcccggctgt
     1381 ctgcgcagcg ccgcatttga cgcagtcatt ggccgaggag cttcgatcga ctatggtcga
     1441 gccgcagagc agcgagccga gggtcagcga tgcatccaaa acatttcttc gccgaattaa
     1501 agcggcgcaa tgtttaccag atcgcggtcg cttacgccgt gattgcctgg ttgctcatcc
     1561 aggtcgccac gcaggtcttt ccctttttcg atattccgaa ctggatcgtt agggtggtcg
     1621 tcctgctgct gatcgttggt tttccgatcg ccttggtttg ggcgtgggcg ttcgaacgga
     1681 cgccaggtgg aatcaagcac gagcgagatg tcccgcgcaa cgaatcgatt acccgccgga
     1741 gcgggcgcaa actcaccgct ctgatcgtcg tcgtcgcggc cgcggcaacc ggggtgatgg
     1801 ccgtccgatt cttgcgttcg caggaagaga gcgaaaacgc acaacccaaa gctgcaaccg
     1861 agaacaaatc gctcgccgtc ctgccctttg aaaacctcag cgccgaccag agtaacgcct
     1921 atttcgtgag cggcatgcga gacgaaatcc taacctctct cgccgaattg cgcgatttga
     1981 acgtccgctc ccgcacctcg acgcagagat atccaagtca tcccgaaaat ctggaagctg
     2041 ttggccgcga gctggaggtc gcgacggtgt tggagggaag cgtgcagaaa gttgctgacg
     2101 acgttttgat caacatccag ctcattgata cgcggagccg caagcagctt tgggggcaaa
     2161 gctatcggcg caagacgggg gacatcttta gtgtgcaggg cgaagtcgcg gagcaagtgg
     2221 ccgaggcgct caagataaga ctcgctccgg cgcaggaaca gcggttgctc actccgccaa
     2281 cgaccagtgc gcgcgcccat gatcttcttc tccgcgccca tgccttggga gcgcattccg
     2341 atgagcagag tcttgagggg aaaatcgcct tcctgcagga agccgtggcc gatgatccgc
     2401 actacgccct ggcttggggc gacctggccg gcgcttatct caccatcgcc gatgcctatc
     2461 gggccccgct cgacgtctta ggcccgatgc gccacgccgc gctcatggcg gtgaagaatg
     2521 atgacggggc gggcgtcggc catatctggt taggcgccgt ctccatgctc tacgatcgaa
     2581 actttcccct ggcgaagagc gaattggaac gggcggtcgc gctcgatccg aattcctccg
     2641 acgcgcaccg ctggctcggc tggtatctcg cgcgggtcga acgcgacttc gacgccggcc
     2701 gcgcggaact gcaaagagcc cgcgcgctcg atccttccta cacctggcca ctcatgtttg
     2761 aatgctacgt cgcgatcgca cgcggcgatt atgcatcagc cttgcagctc gcccagcagg
     2821 tcatggaaat cgatccccac tttttttacg atgtcgaccc gatcgcgagt gtttatgccg
     2881 ccgagggtcg ctggccggat gcgatcaaac gttacgagtc gcttcccgct gggacgatca
     2941 cgcggccgaa tttccagctc gcgatctgtt atgcgcggac cggccagagc gaccgggccc
     3001 gacacatcct ggccgacctc gaaacgcttg cccagcaacg ctacgtggat cgctcccatc
     3061 tcgccgggat ctatgcggca ctcggcgaca aggacaaggc cttcgccgca cttgatcagg
     3121 cgtgcgccga tcgctccgcg cgggtcagca cgccccgctt ttacacttgg ctggcgcccc
     3181 tctttgacga tccgcgcttt gccgccttgg aagacaaagt cgcgcactcc gcgctggcgt
     3241 cgcctctcga agtgcagccg tgagcctggt cggctgggac gagccacgcg gtcgaggctg
     3301 gccggtcgct gcgcggattt gcggtctccg tttctttatg catgctgcgt aatgcatgac
     3361 gcgtaatcgg attaaccctt tcgaatatgg cggggtggtc gccggagatt ccttctgcaa
     3421 ccgggcccgg gagaaagcgg atctcgcgca ggcgatccgc aaccatgaaa aactgttcgt
     3481 tttctccgag cgtcgctttg gcaagacctc cctcgtgcgg gcggtactgg ccggcttctc
     3541 gaaaagaacg acggtttgcg cctacgtcga tctctggccg acggacaacg aggcgacatt
     3601 tgtgaccgcg atggccaaag ccatcacgca agcgatgagc tcgtcggtag ggaagctttt
     3661 ggaaaccggc aagagcctct tcagtaacct ggcgccgagc gtcacgctcg acgagggagg
     3721 aaagccggag gtaaacttcg gcgtctcccg gcatacccgg atcgttcccg ccctggacga
     3781 agtcctggaa accccggcga agatcgcggc caaacacgac cgcgacgtgg tcatcgtgct
     3841 ggacgagttc cagcaagtcc tcgaatacgg cagtgaccgg gtggaaaaga agttccgcag
     3901 tgtgatccaa aaccaccgtg aagtggctta cctctttctc gggagccgga agcatctcat
     3961 ccaaaaaatg ttcatggatc gttcccggcc gctctaccgc gcgggtggcc attacccgct
     4021 cgggccgatt gcggaagaac attggatgcc gtttatccgg gagcgatttg cgggggccgg
     4081 caagaagatc agccagggct tgatccattc catctgcgaa cagacgcaag gacatccctt
     4141 ttacactcag catctctctc atgcgctctg ggagttatgc gaacctggcg ccacggttac
     4201 cgaagggttg ctcaaggagg cgctccggct gctgctgcag cgcgaaaatt acgcctactc
     4261 gaccttgtgg gaatctctcg cgatgaatca gcgcaagttc ttgaagggga tcgcgagcga
     4321 agccgcaccg gtgaagcctt tcgccggcga atttgtgcgg cgttccggcc tcggctcgcc
     4381 ttccaacgcg caacgcgcgg tcgatgcgct cctcgaccgc gatgtcatcg atcgggagaa
     4441 cggctcgttc ctgatcaccg accgcttttt caggctgtgg gtgcaaagag tgcaactgcc
     4501 gtgagaccgc gcccgctccg gacagatgaa acagccaggt atgacgcaga gagcaggtct
     4561 catcggaggc gggttttgag tcccggagag gggaggcttt ggcgccggaa gcggggacca
     4621 tgaactcagg gaacttcttc agcgaattaa ggcggcgcaa cgtctacaag gtcgcggtcg
     4681 cgtatgcggt ggtgggctgg ctcctgattc aggttgccac ccaggtcttt ccttttttcg
     4741 agattccgaa ttgggtcgtt aggctggtcg ttctcctgat cgttcttggt tttccgatcg
     4801 cgttagtcat cgcctgggcg tttgaattaa ccccgcaagg atttcaacga acggagatcg
     4861 cggacaagct gccgaaccat tcggcccgta gccgggcatg gatttatgtt gtcatcgtgg
     4921 ctggtctgtt ctccgccggc ttgttcttcc tcgggcgctt cgccgcggcg ccggcgcaga
     4981 gcggcgcggc ggaggcgccg gaaaaatcga tcgccgtcct gccgttcgaa aacttgagca
     5041 gtgacaagga aaacagctat ttcaccgacg gcatccagga cgagatcctg acccgcttat
     5101 ccaaaatcgc cgacctgaag gttatctcgc ggacatcgac ccagctctac aaaagcgcgc
     5161 cgaagaatct tcccgagatc gcgaagcagc ttggggttgc ccacatcctc gaaggcagcg
     5221 tgcaaaaaag cggcgaccag gtgcacatta acgtgcagct gatcaaggcg gccgaagatt
     5281 cccacctttg ggccgatagt tttgatcgca aactgacgga cattttttcc gtcgaaaccg
     5341 aggtcgccaa gaccatcgcc gatcagttac aggccaaact gacgggtcag gaagagcagg
     5401 tcatcgccgc ccggccgacg gagaatcctg aggcctacga tgcctacctg cgcgggctgg
     5461 cctacaatct gaaaacggcc aactcagccg ctaactctcg cggcgcccaa aagtacctca
     5521 gagaagccgt acagttggat ccgaagtttg cccttgcctg gacgctgctc tcctttgtcg
     5581 atgcgcgtgg ctatctcacc cagactctgc aacccacgga tgccctccgg gaagaagcgc
     5641 ggcaggcagc gacgaccgct ctcactctgc agcccaacct cggcgaggcc ctccatgcca
     5701 agggttacta tcattacgcc tgcctgaagg attacaccac cgcggtgcgc tactttgaac
     5761 aggcccggcc gttcctgccc aatagcagcc ggattcctga atcgctggcc tatgtcacgc
     5821 ggagacaagg ccagtgggac aagagcgagt cctatttcaa cgaagccgag cggctcgatc
     5881 cgcgaaacgt ttacgtgctc gggcaacatg gccaatccta catcgctctc cgtcgttttt
     5941 cggcagcgct acggaagttt gaccagattc tcacaatcac accggacgac atggatacga
     6001 tcgcggtcga ggcgtccatt gcccaggccg aaggagacct ccggcgggcc gccgcgctcc
     6061 tcgctccgct ccacccgccg gccgacgatt ccaatgtctt attcacgcag gtttaccagg
     6121 ctatcctgga gcgccgtcct gcccccgcga tcccgcacct aacgagcctg ctcgccaaac
     6181 cagacccggc gtggggctac tttacggggg aactgcgctt ttggttaggc tgggtgcagg
     6241 atgtcgccgg cgaccacgcc gccgcccagg gaagttggcg ccaggcccgc ggcgagctgg
     6301 aacccttcct cagagaacag ccggataact ataacctcct cttcgatctc gccctgaccg
     6361 atatgggtct cggtgacaaa gcggtggcgt tagccatgtc ggagcgggcg atggccgcga
     6421 tgccgatcga gaaagacgcc tttgatgggc ctgccccgat cgagatcctt gcccgggtcg
     6481 cggctcaggt cggagaaccc gatcgcgcca tcgccgccct gcaaaaggtt ctcgcgctcc
     6541 cctacgaggg cccgctcggt ggagggcagg cgccgttgac ccccgcgctc ctccggctcg
     6601 atcccatgtt cgatccgctc cggaatgatc tgcggttcca gcaactcgcc gccggccagc
     6661 gatgaacttt ttctccgagc tcaaacgacg caacgtctat aaagtggcga ttgcctatgc
     6721 ggtggtcggc tggttgttga ttcagatcgc gacgcaggtt ttccccttct tcgagattcc
     6781 gaattgggtc gttaggctgg tcgtcctgct gatcatcgtc ggcttcccga tcgcacttat
     6841 tatcgcctgg gctttcgaac tcaccccggg cggaatcatg cggacggaag cggccgatcg
     6901 cgtcccggcg gcgcctcgaa aaacgcacac ctggctctac ctcgtcgtta tctgcggtgc
     6961 tttctccgcg gccttgtttt ttctcggccg ctacaccgcc agcaatcacg agaacacttc
     7021 cgggcgcctc gcgaaatcga ttgccgtcct gccttttgag aatctgagtc cggacaaaga
     7081 caacgcctat ctcgcggaag gcatccagga cgagatcctg acccgtctcg caaaaattgg
     7141 ccaactgaag gtcatctcgc gcacttcaac cgcgaaatac cagagcggac ccgacaacct
     7201 gcgcgagatc gcgcaacagc tcggagtcgg gaacattctc gaaggcagcg tgcaaaaggc
     7261 gcgcgaccag gtgcgcgtca atgtgcagtt gatcgacgcg accaacgatg ctcatctctg
     7321 ggcggatagc ttcgatcgca ggttgaccga catctttgcg ctcgaaagcg agatcgcgaa
     7381 agcggtggcc gacagtttgc aggcgaagct gaccggctcg gaagcgcacg ctctcgccgt
     7441 ccatccgact gaagacccgg aggcgcatcg gctttatttg ttaggccgct ttttttggaa
     7501 caagcgcacc ggtccggatt ttcgcaaagc catcggctat ttccaacagg ccatcgacaa
     7561 agatcccggc tacgctttgg cctacgcggg cctggccgat gcttatgtcc tgctttccgg
     7621 ctactccgcg gcgacgccga aggagagttt gccggaggcg aaggccgcgg cgcaaaaggc
     7681 gctggaactg gacgagagtc tcggggaagc acacgctgcc ctcgccgagg cgattttcgc
     7741 ctatgatttc gatgtcgccg gggcgagccg ggagtttcgg cgcgcgctcg agttgaatcc
     7801 gaactacgcg acggcccacc aatggtatgc cgaaagtgcg ctcgcctccc aggggcgttt
     7861 cgacgaggct gtcgccgaga tgcggcgggc gctcgagctc gacccgctct ccgtcgtcat
     7921 caacgcggac gtcggcacga tcctctgtac ggctcggcgc tacgacgagg ccatcgaaca
     7981 gttgcgtaag acggtcgaga tggaccccgc tttttattac gcgcactgga acctgggtca
     8041 ggcgctggaa ttgaaaggcc agctcaagga agcccgggcg gaatatgaga aggcgattac
     8101 gctgagtgac gatccgctgc cgcgggcgtt cctcgccgaa ctctgcgcga agactggcca
     8161 gaaagagaag gcgcgggaga ttcttgccga gttgcgtcgt gtcccgaaag acacctacgt
     8221 ctcgccctac aacttcgccc tcatttacat cggtctgggc gaaaaagatg aggctttgcg
     8281 ggagcttgag aacacctatg aggaccgcga cggatatagc attgtgttta tcaaagtcga
     8341 tccaatgctg gatcccttgc gcggcgatcc gcggttcgag gcgttggtcg agaagacagg
     8401 acggcctttc gccgcggcac agtcaggaac aaatgggcgg atcgatcgcg caacgttcgc
     8461 gctgtgccgt gttgagctta ccggagcaaa tcccttttcc gatgtcagcc gcggcccagg
     8521 aggaaagcaa taacgtgtta tcgacaagag gttatgccgc ctataacgca cgagaaccgc
     8581 tgagaccgtt ctcgtttgag cgccgcgagc cgaaagcgac tgacgtgcag ctcaagattc
     8641 gggatttcgg cggacgggat cgcggtggac gcctttgatt tgattgcgcc gcgccggcag
     8701 ctggctggtt ccctgatcgg cggcatcgcg gagactcaag agatgctcga cttctgcgcc
     8761 gaactcggta tcgcttgcga gatcgagctg attccgatcg accagatcaa tgaagcctat
     8821 gaacgcatgc taaagcgcga ggtgaaatat cgtttcgtcc tcgacatggc gtcgctgcga
     8881 tagcaaagac atgttatgtc actcgggtta gcccggcggc cacgacaaga atgaattggg
     8941 atgcgatcaa tgcgatttcg caactggtta gttcagttgc cgttgtcttg tcggtgcttt
     9001 atctcgccaa agaagtgcac cgcagtacgc gcgtcgcgaa ggtagccgcc caggattccg
     9061 ccgcctcggc agtccgggat gtgactaaca cgttcatgga aaatgcggat atgtcgcgca
     9121 tttggggaac gggtcttgaa gacatcaaaa cgttgtcacg ggaggaccag gcccgtttct
     9181 ttcacgccgc ccaccagttc ctcaaggcgc tggagaccat tcactttcac tatgttaatg
     9241 ggttgatgga cgaacagctc tggcgcggct ggcaggaact acttcggcac tatatcgtcg
     9301 cgcccgggat tgcccggtat tgggagatcc gcgatcagct tttttcggcg cgctttcgcg
     9361 aattcgtcag ccaactcgaa cctctccgcg atcgccgaac tgtcggcact ctgctggaac
     9421 aggaggacct tccggagtaa ggagagtcct tcccgcgcgc atctctctta tcgccaccgc
     9481 catctgctcg acggtctcga cctcctctac cacaaaccga tgtggtaacg ccctctgatc
     9541 aatcaattgc acaacccgcc caacacccgg cttcaaccag aaagagatgg gatcgtcgcc
     9601 tttggtcagg attttcgaga ggtgctggcg gcgcttgcgg cgtgttgctc aaagctgata
     9661 ggccggcacg ctcgttcctc gaagtggttt caacgcttcc tcagctttgg tcagggcagc
     9721 gccagcataa acatcccccg gccgaaggtc gcggcgtaga gcgcgttgga ggcggcccgg
     9781 tattcgagat cggtaaccgg gacgttaggc aggccggtcg cggcggcgcg ccactttcgt
     9841 ccgccgttgg ccgaactgta cacgcccacg tcggaagcca cgtagagcgt cgaaccgacg
     9901 accacgatgt cgttcaccgg agcctgggga aggttgccga cgatgctcga ccatgtggtc
     9961 ccgccatcgg ctgtctttag gacgtagggt aactccacgc cggaccggaa acccgagaag
    10021 gtggcgtaag cgacctgcgc gttcaccggg tcgaccatta cccgggtcac ccaggttcca
    10081 ggcacgtcgg aatcggttac ctgcgtccag ttcgttccca ggttggtggt aaaccagagc
    10141 cggccgtcat ccgtcccggc caacacgcgg ttcgaatcgg tcttcgctcc ggccactgtg
    10201 gtcacggtgc cgaacggata gttgggatcg cgcccgggtc ccccggtgag gtctgggctg
    10261 atgaccgacc agtgcacggc atggtcggag gatcggttga cctggttacc cgcgtaataa
    10321 aggaccgccg gattggccgg atcaaactgg agcggggtaa gccagttccg ccgggaggag
    10381 accgtggcgc tagtaaagtc ggtgctggag tcgccgccgt tggtcgagcg tagacagtcg
    10441 ccgtactggc tgcatccgta gaccatgttc tggtcggagg gatcgatcag cgcttcctct
    10501 ccgtcgccgc cgatgtaggt gttccatggc gtcccgtcgt aggagcggtt gacgccatta
    10561 tcctgcgctc cgccgaccag ccgggtgctg tcctgctcgc tcacgtccac gctgtagaac
    10621 tgcgtgaacg gctggctgac cgcgaaggtc cattggtcgt tcgagccgtt aacgtccgag
    10681 cggtaggtgc cgccgtcatt cccgagatag acgcggccgg gcaccttgag gtcccagacc
    10741 atggcgtgat cgtcggcgtg cggactgaac tctcccgtga atgtcgagcc gctgttcttc
    10801 gactcacaga ggtagactcc ggccccgaag acgtgcgtct ggtcgagagg atcgacccac
    10861 acccggccga accaccaacc ataggtcgat tgcgcccccg agagcgttga gctggtgggg
    10921 agtttggtcc aggtgtttcc gccgtcggcg gatgtataga agccttggaa gagtccgttc
    10981 gtctgattga caatcgcgta gagccgctgc gggttcgaca cggccagagc cactccgatc
    11041 cggccgatcg tagtgccggc ggccggaagc ccattcgtta ggcgctgcca tgtcgtccca
    11101 ccgtcaatcg atcggtaaac acccgacccg acgccgccat aagtccggag gtcgggttcg
    11161 cggaagtgat cccacatcgc cgcgaagacg cggttcgggt tggtgggatc gatcgcgagg
    11221 tcaacggctc ccgtggtgtc gttgtcgccg gccagcacct gtgtccaggt ggagccgcca
    11281 tccgtcgatt tgtagacgcc gcgctcgccg ccgtggttgt agagttgccc ggcgacggcg
    11341 gcaaagatgt tctgcgagtt agtcggatcc accgccagcc gaccgatggc gccagagctc
    11401 gttagtccaa cgagcagcca ggttgcgccg ccgtcgaccg atcgatagat gcccgatcca
    11461 ccgtaggtga tgcttccacc gcccgggtta gcttcaccag tgcccgcaaa cagggtcccg
    11521 ttcgaagtga tgagcagcgc acccatcgac tgcgggttcg tcgccggcca gatcgcggtg
    11581 aaattggcgc cccggtcggt gcttttccac acgcctccag tggctgcggc cacgtaaatt
    11641 gtatcagcgg ctactggatc cacggcgatg tccaccaccc ggcccccgat gttggtcggt
    11701 ccgacgaacc gccagcgcga ccgggtctgc gggcctgact gctcgctggc cacggcaaga
    11761 gcggctgctt gcgcgccagc cttttccagc gcgccggcag ggattccgcc atgagtcact
    11821 cgctgcgtaa cgaaccagtc ttccggcggg ctctctttgc tcgcgcgcgt ctccccgtta
    11881 atctcgctcc gtcgccgctc cgcgccggcg gacccgggga gcgaggtcac ctggaacgcc
    11941 gccgtcgcca gcagcaggac cgccagaagc gccaggattc gcgcaagtgc acccctcatc
    12001 gctcctccct ctcggacgaa tgattattca tgaggcgcat tgtgggcaga acagggcgac
    12061 agcgtcaagc tcgtcgatcg ctcaccaagg ctgacaacgg aactccgaac gctaaaagcg
    12121 gagcgaccgg ccgtttttca ttactgcgcc atgtcgagca cgacccggaa ccgcgccttg
    12181 ccgctcatca ttcggtcgta ggcttcgccc acttgcgaca aagaataaat ctcgttcctc
    12241 gatttcaccc cggcgagcgc actaaaccgg agcgtgtcct gggaatcgat cgacgtgccc
    12301 gaataccagc ctttgaccga gcgcgcgccg ctgatcagca ggagcggatc gacttgaatc
    12361 ggacctgccg cgccgatcac catgaagacg ccgttaggcg cgagaccgcc gatcgttgcc
    12421 gccatcgcgt cggcattggt gacggtggcg atcacgactt tcgcgccgcc gagtttctga
    12481 agttccgccg ccggattttg cgtctggctg tcgatgtatt gcgccgcgcc cagctctcgg
    12541 gcgagcggtt ccttgtcttt cccgcgcgcg atcgccaccg tccggaagcc catcttcgcg
    12601 gcgtattgca cgccgaggtg accgagcccg ccgatcccga gcaccgcgac caggtcgccc
    12661 gcacgcgcgg cgctgttgcg cagcgcattg aaggtcgtga ccccggcgca catcagcggc
    12721 gcgctctcca cccccgacaa ctcgttcggc atcgaggcca gcgctttggc cggagcaatg
    12781 aggtattccg cgtaaccgcc gtcgaaggaa atcccggtcg tcagaagagc gatctgacag
    12841 gcaaagaaat caccgcgccg gcaggaatcg caatagccgc aatgcccgcc gtgccagcca
    12901 accccgatcc gctgcccgct tttccatccc tccacgcccg agccgacggc gtcgaccgtc
    12961 ccgatcactt cgtgcccggg cacgcgtgga tattggattc ccggccacag cccatcctta
    13021 actaacgtgt cgctgtgaca aatcccgcaa gcctggacct tcacccgcac ccaccccgcc
    13081 ggcggctccg ggatttccat ttccacgatt tcaaacggcc ccttcgcctc cgggacctgc
    13141 actgcccgca ttttttttgc catgcttttt tatcggcctc ttctttcgaa aaacaagact
    13201 tcgattagaa ggtgatccgg ccgcaacgga tcgcgcctaa cgagcgggac cgtgcccgtc
    13261 tttaggaagg attttcgact ccgtccttcg gtaagcgtcc tcggctcgcg cccatcgggt
    13321 ccttctccgg tcgcaaatcg cggagtttgg atcgggaccc ttccggcgtc gtctccggcg
    13381 ctccctcgtc ccggctttcg cccgtctctt cgtcaggctg tatttccatt ctcccgattt
    13441 tcgtgcgcgc gagttttacc gtcaagattg cgctctaatc tggcgacgga atcatggggg
    13501 gcctggtccg catcatgatc acccctcgac cgaggaccgc gacgagcacg agcagcccgc
    13561 cgacgatcgc ccacgggccg ggtttttcgc cgagcgcgag catcacccag agaggattca
    13621 acaccggctc gaggagcgga atgagcgttg cctcgagtgc ggtgacctgc ttgatcgccg
    13681 cggcgtaaaa gacatagggc aaaccgagct gcacggtgcc gagcagcgcc agccgccaga
    13741 ggctgccttc gcctaacgac ggtgcctgga acatgaaagg cgcgccggcc agtgcgacaa
    13801 tcacgttgcc gagtacgatg ctcgcgattg gcgacccggc tttttccttg cgcaaaaaga
    13861 gcgcgaccga cgcaaacgca agtccgctcg ccagcgcgat gatgttgccc cacatcccgg
    13921 cggtcgtcag gcggtcgaag aagaaaaggg cgatcccgag cagggcgacc gcgatgacca
    13981 gccaatccac ccgcgaggcg cgctcatcca gataccaccg cccgatgagc gcgacgtaaa
    14041 tcggcgcggt gtattgcagg aagatcgcgt tcgccgccgt cgtcatccgg gtcgcgaaga
    14101 caaagaggat gaccgtcccg gcgtagccga gcgcgccccc gatctgcgcc cgggaacagg
    14161 tgaagcgggg tcgcccgatg cagagcagga tcaccggaat ggcgatcgcg ctccggccgc
    14221 cggcgatcgc cattggcggc caatcgatcg acttgatgag cacgccgccg aggctccagc
    14281 agagtgccgc gagcaggagc agaacaggcg gcagaaaacg gcgcggcatc ggaatgccag
    14341 ccctttacaa ccgcaacgtc gccgcgcaag cggccttctc ttgaaacgcg ccgatttacg
    14401 tgagctgggc ggccaggatg gcgcgcccta caccgcgcgt gtaacctgcg ctccgatgaa
    14461 atttctcgtg accaacgacg acgggatcga tgccgcgggc ctcacggccc tggtcggcgc
    14521 cgccgaaact ttgggcgagc cggtcgtggt cgcaccggcc ggaccgcaat ccggagtgag
    14581 ccatgccgtg acgtggcgcg aaggcgtccg gatcgaaccc cggggcgaga atcgtttcgc
    14641 catccacggc acgccggccg actgcacccg gctcggactg ttgcacatcg tgccggacgc
    14701 gaaatggatc ctgagcggga tcaatcacgg cgcgaacctg ggtgccgatg tctattactc
    14761 gggcacggtt gcggcagtgc gcgaagcgtt gctgcacggt tggccgggca tcgccttctc
    14821 gcaataccgc aagagcggcg tcgattgtga ctgggaacgc gccacgcgct ggatcacgcc
    14881 cgtccttgcc gacctgctgg ccaagccgat cgagccgggt gttttctaca acgtcaatct
    14941 gccgctgctt gcgccggacg cgcccgatcc ggaagtcgtc tggtgtccgc tcgatccgaa
    15001 acccttgccg ctcaattacc ggcacgaagc cgagagcggt ctctactacg cgggcgacta
    15061 caatctgcgg gaccgcaccc tgggagccga cgtcgacgtc tgcttcggcg gcaacatcgc
    15121 cgtgacggcg gtcaggttgc tgtgaccgcg gtatgcaccg ttccctaacg aatcagcgct
    15181 cgcgcttctc tcgttagggc cgcttcgcgc cggcgttggg aaaacgtctg cggccggcgc
    15241 caattggatc tttctcggga cgcaaatcgc gcagcttgag aagaggcggt tctttaatcg
    15301 gctccgttac ctgctggctc gcgtggttgc tgatttcttc gtttggttcg ttgtccattt
    15361 tttggatttt tgtgcgcttg aatttgccta catccccgcg ggatgtcgtg cgacgtagcc
    15421 acgggtatcg cgcaggtccg cgtgccagcg cggaccggtt gtgccgccgt cctggaagtc
    15481 ggtggtgtcg agccgcaacc cagcgatgac cgcgaaggtg tgaccggggc gcgcgtagat
    15541 ggtgaaccag cggccgcggc cgcgttcgcc gtagcgcatg aagtcgttcg agggaagcgg
    15601 cacggcgatc gcaccggcgc cgtgcagagc aaaagaaacc gtgcccgaac aatcgtaacc
    15661 acgatcataa aacgagccat gaccgccgcc ccagatgtaa ggtttgcgat gcagggcgtt
    15721 cgcggcccag atcgcgttct ttacgctttg tggcgcacga gacggggcag aggcgatccc
    15781 gccgcggaga atcgcgcgac tgcctgcgac ggtcggccca tggacggatt ttgccggagc
    15841 gggtttagcc ccctgctctt gatcgagatc ctggtcgtct tgcggtggtt cagggttccc
    15901 gggattctgc gaacgcggaa ccatgtaggc aacgcgcaac ggctggtcgt agccggccga
    15961 ttgcgccagc ggaacggcgg ggcgcgcaat cgcctcgtct tcctccacca tttcaccacg
    16021 gtaaacttcg cggttgcgct cgctgcgata cgcgacccgg atcggcgcag tggccacaac
    16081 ggcaattgcc cgcggcgcgt tgcgcacgag cgggtcggcg acggtgtgaa cgacggatcg
    16141 cgcgaccagg aaaggttgca gtgcgaccgt gaccgcgacc ggtctgggtt ttcgatgacg
    16201 ttgcgtgcgc gcatcggccg gcgcgaggag ggaaagaaac aagagcacga ccacgcacga
    16261 gaccgcgcct cgtcgcgcag cgggtatacc acagcccggc gtctgcacgc cttaagaaag
    16321 aagctgttct cgggccagct ccgcaggttt tcctggcagt ttagccccta aagaaagaaa
    16381 gcgacggcag cccagcaacg ggtcgcccgc tcggactgtg acgcagaacg agtcgtcggc
    16441 gaatccagac gcaaggcggc cagagaataa tctctggccg ccaatcgcgc ttctgatttg
    16501 aagatctgag ccgcctacct tttgccctgg gtgattacgc cgacgaggcc attgccggcg
    16561 aagttcgcaa gatccgggcc ggcgtcaaag taaagctcca cgctcccccc accgccatcc
    16621 gattgcgtaa agatgaggtc ccatagccca ggtatcacaa tgtcgttccc gtcacgatcc
    16681 tgcatcgcgc cgagaaaatg gcctgagcta tcgaacgcat taatcttacc atcgtcgatg
    16741 tttccgatca gaatggcgtg actgaactgc ccgaagtgag ccggggcttt ggccacgccc
    16801 catgggttca ccaaaggacc ggctccgccg gcgttcgcgg caaagtgatt tggcgtaagc
    16861 aaattgccgt ccgtgtcgaa tatgtccacc acacccccaa taggcgcgcc cgtggtcacg
    16921 ttaaaggtga cgaagagctg cccatcgacg ttctcgactt gagaagcggt gtagtccgga
    16981 tactggccgg cgacattcgg atcggtgaag gaggcgaggt aattgaactg ctcgtcgtac
    17041 atgtccaccc gattgttcgg gccggcgtcg caggcgtaga ggatggtctg cccctgactg
    17101 tttgtgccca tgacgagccc ggtgtagttg gacatgaagg gcagctgggt ggagttgtcc
    17161 acggcaatga tggcgttgtc cgggtccacg t
//
LOCUS       DATMNS010000207         2294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2800370, whole genome shotgun sequence.
ACCESSION   DATMNS010000207 DATMNS010000000
VERSION     DATMNS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2800370"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(290..1366)
                     /locus_tag="VNW28_07070"
     CDS             complement(290..1366)
                     /locus_tag="VNW28_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXA09727.1"
                     /translation="METLIEEPPAKASRVPRRYSASVLITVIALHLVFGSVAAIWVVS
                     RYSAARKLTFNAGPKSPNPSERALEHRVQLQKKMQSVSAPPAVPKRVLTTGAAKIVLP
                     PMPEIKAAENPPRSMMSASGTGTAFGTTGGNSLGGGGGGNGSQINFFGIRDVSTSVVI
                     MIDVSDSMFTRTGDAQYPSKLVRHGKEQNFQVVRDEAIKLMQSLTPQTRFGIVRWSGG
                     AHPWKTELVPATEENKQAAIAHIQNEVDYKTARPPRGKPGGTRHDLALEEAFSLKPET
                     IYMLTDGNATVSQPGGGLKPIPPQEIYKVAEEGQKELSKRAHLHVIYYLSGKEKPDEV
                     AMLRGLASRNGGKFQKVEAKGRKK"
     gene            complement(1505..>2294)
                     /locus_tag="VNW28_07075"
     CDS             complement(1505..>2294)
                     /locus_tag="VNW28_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HXA09728.1"
                     /translation="FTFASTFHVSPNDFEKDASGRLKRERLAGAQAGSDTKEYLVYHK
                     TSPESGPAGRYLVNDQGTPVYLVDPGINGIYEKRPDGSSVQKFSAPKATLMSYIIKGI
                     LSGQLPWGLVLLGVFISIVLELAGIPSLAFAVGVYLPISTSAPVWVGGFIRGLVDNYT
                     RRKHAGANFTEDQLIAEGDKSNGVLLSSGYIAGGTLAGVIFAFMNIPLKDKLDVIEKW
                     ATAHNPFFNGPHADLLALLPFAVLCVLLYLVGRDVLLAPKKRKI"
BASE COUNT          422 a          687 c          696 g          489 t
ORIGIN      
        1 gccgcttcgt cagatcgagc gaatttttct cgcgaccgag cgcgatcgag atgtctttca
       61 caaatttatg gaacttctcc ttcggcgatt tccgttcctc ttctttgatc tcgttgaggt
      121 tcaccttgtg catgggagaa aatttggcga ggagcggcca aaggcgagca aaatgtagcc
      181 gccccccctc tgggaggctt gggggtaggg atgaaaaatt tgaagaagcc tcagaacggg
      241 ccgcgcctgc acccgcgctt cgcagagcga agcgtctacc gggccggccc tacttcttcc
      301 gccccttcgc ttcgactttc tggaatttcc cgccgttccg actcgccagc ccgcgcaaca
      361 tcgcaacctc atccggtttt tccttgccgc tcaaatagta gatgacatgg aggtgcgccc
      421 ttttcgatag ttccttctgg ccctcctccg ccactttgta aatctcctgc ggcgggatcg
      481 gcttgagccc gccgccgggt tgcgaaaccg tcgcgttgcc gtcggtcagc atgtagatcg
      541 tttccggctt gagggaaaac gcttcctcga gcgccaaatc gtgccgtgtc ccgcccggct
      601 tgccccgcgg cggcctggcc gtcttgtaat cgacttcgtt ctgaatgtgc gcgatcgcgg
      661 cctgcttgtt ttcctcggtc gccggaacca gttccgtctt ccacggatgg gcgccgccag
      721 accagcgcac gattccaaag cgcgtttgcg gggtgaggct ttgcatcagc ttgatcgctt
      781 cgtcccggac gacttggaaa ttctgctcct ttccgtgccg gacgagcttg ctcggatact
      841 gcgcgtcgcc ggtgcgggtg aacatcgaat cagagacatc gatcatgatc acgacgctcg
      901 tgctgacatc gcggatgccg aagaagttga tctgggagcc gttgcccccg cccccaccgc
      961 ctaacgaatt accgcccgtc gtcccgaagg cggtgcccgt gccgctcgcg ctcatcatgc
     1021 tgcgcggcgg gttttccgcc gccttgattt ccggcatcgg tggcaaaaca attttggctg
     1081 cgccagtggt gaggacgcgt ttcggcaccg cgggcggggc gctgaccgac tgcattttct
     1141 tctgcaactg gacgcggtgc tcgagcgctc gctcgctcgg gttgggcgac ttcgggccgg
     1201 cgttaaaggt gagcttgcgc gcggcggaat atctcgagac gacccaaatt gcggcgacgc
     1261 tgccaaagac gagatgcagt gcgatgacgg tgatgaggac cgacgcggaa taacgacgcg
     1321 ggacgcggct ggctttagcg ggcggttctt cgatcagtgt ttccataccg agtcggtcat
     1381 cctaagccgc cgcgcggagc gagcaacgtt gtcgccgctt cgctctgcga agcgcgggat
     1441 gtgaaaattg caatccgcgc aaccctcgcg tcgcccacag ggcgacggct acacgggtca
     1501 cgcctcagat cttgcgcttt ttcggcgcga gcagcacatc gcgcccgacg aggtagagca
     1561 agacgcacag cactgcgaac gggagcagcg caagcaggtc ggcatggggt ccgttgaaga
     1621 acggattgtg cgctgtggcc catttttcga tcacgtcgag cttatctttc aacggaatgt
     1681 tcatgaaggc gaagatgact ccggccaggg tcccgcccgc gatgtagccg gacgacaaga
     1741 ggactccatt gctcttgtcg ccttccgcga tgagctgatc ttcggtgaag ttcgcgccgg
     1801 catgtttgcg acgggtgtag ttgtcgacca gcccgcgaat gaagccaccc acccaaactg
     1861 gcgccgaggt ggagatcgga agataaacgc caacggcaaa ggcgagcgaa gggattccgg
     1921 caagctcaag cacgatcgag atgaaaacgc cgagcagcac aagtccccag ggcaattgac
     1981 cactcagaat tcctttgatg atataggaca tcaaggtggc tttcggcgcg gagaacttct
     2041 gcacgctcga gccatcgggg cgcttctcgt aaatgccgtt gatgcccgga tcgacgagat
     2101 agaccggcgt cccctggtca ttgacgaggt aacgtccggc cggcccgctt tcgggtgagg
     2161 tcttgtgata aaccaagtac tctttcgtgt cgctgcccgc ttgcgcgccg gctagtcgct
     2221 ctcgtttcag ccggccgctc gcatcctttt cgaagtcgtt tgggctgacg tgaaaggtgg
     2281 aggcgaaagt gaaa
//
LOCUS       DATMNS010000208        40662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_644302, whole genome shotgun sequence.
ACCESSION   DATMNS010000208 DATMNS010000000
VERSION     DATMNS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 40662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40662
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644302"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2031
                     /gene="secD"
                     /locus_tag="VNW28_07080"
     CDS             <1..2031
                     /gene="secD"
                     /locus_tag="VNW28_07080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXA09729.1"
                     /translation="SPVGTDRILVQIPGLDPAKIIEARQQLSRVAKLEFRLVYPDNGE
                     RLRAIDAGTEVIPPEYRIETYKMQQAEGQKPVEERLLVKKKADLGGDRVTESNAYYGN
                     EGWTVQLKFDSEGAKKFGQITEANVGHRFAIVLDGVIQSAPSIRTAIYGGDAIITGHF
                     GEQEARGLASVLENPLQTPVSVEEERSVSPTLGLDSIRASIFAGLVGLAITLVLVMAY
                     YKFAGLIANLVLIVNIILLMGALTMFNFVLTLPGIAGIILTIGLAVDASVLIYERLRE
                     ELALGKSLKIAVQTAYEKAFSSILDANVTTLITAAILFWKASGPVKGFAISLTLGILA
                     SLFTALIVGRNLFSWFVDTGKVKRVSMLHLISSKNINFLGKGFIATMCSLAMLIAGGT
                     AFVIRGDRNFGVDFRGGDLLSLSAAKTVQVAQIREALKPIHLDNEPIQQSLEGNRNFI
                     TIRSPINTSDQVAKAIKEYIPNGEFKVEREERVGALVGGELARNSLWALGLGIFGILI
                     YVTFRFELSFAVGAIVALLHDVLMTVGVFSLMGRELTLTMVGAVLTIAGYSINDTIVV
                     YDRIREGLASGRRGSIEQIMNESINQTLSRTILTSTVTLIPILCLFLFGGAVLRDFSL
                     AIIIGVAVGTYSSVFIASPIVLWWTRARGGSASSLRREVTQKSAVTSPSSAS"
     gene            2125..2322
                     /gene="rpsU"
                     /locus_tag="VNW28_07085"
     CDS             2125..2322
                     /gene="rpsU"
                     /locus_tag="VNW28_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXA09730.1"
                     /translation="MPEVIVRKGEPVDRALKRLKNKLDAEGILEEVRRLRAFETPSQK
                     TRRKAKANAKRGRAKFRFNPS"
     gene            complement(2490..4076)
                     /locus_tag="VNW28_07090"
     CDS             complement(2490..4076)
                     /locus_tag="VNW28_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXA09731.1"
                     /translation="MVSFPIPLPDDPRKWDGWSRYDSPDLYERLCLDPSTNPSNELIE
                     ERFRHLVLWWQKKLPLKNQPSNPLAQLLRSGLEDSSRFLTEARVELLNPERRQKVDDE
                     LAAQAQEQAITEFNKYLAFALNDKTLTPQEEQTLLEFGREQDLADERMMELIEAELLA
                     CGGARLSRPSVEETAAAGNNRPALNPEDDFMRMLRLSGLGSDEMTDDQRDAFVNMAEN
                     LGIEPGDAEDMVDRYLEEIEGQAALPATKAVPGVVAPKPEPVPASTAPTLSAPAVIDP
                     NAERAKYSNFVNTIGAEIFLVPSGVFKMGSEAADAAPNERPITQVTLTRYYLSRHLIT
                     NAQYEMFDPSHARKRMARTGDRHPVVYVSSGDAMKFCQWLSVRERRRYRLPTEAEWEY
                     AARGSDGRKYPWGNHEGRGNLANFADRNTTFAWSDREIDDGHAETSPVGAYPLGSSPF
                     GIEDMAGNVWEWCLDFMEGYRGTPKINPRGAVSGSKRIYRGGSWKSRFHSLRATTRGA
                     NAPNYSCNDLGFRIVCECDM"
     gene            complement(4089..5651)
                     /locus_tag="VNW28_07095"
     CDS             complement(4089..5651)
                     /locus_tag="VNW28_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HXA09732.1"
                     /translation="MSNRKAVGIDLGTTFSAVAHIDAYGKPQIIPNSESERITPSVVL
                     FDGSNAIVGTIAKNNTVAEPEKIVDFVKREIGKAKEQFRREFSGVAYSAEELSALILK
                     KLKSDAEKYLKTPVTDAVITVPAYFNDAERTATITAGRLAGLNVLQIINEPTAAALAY
                     GLDKLDEDQTVFVFDLGGGTFDVTVMRIEGHRLEMVATNGDHRLGGKDWDDVIVNYVA
                     EEFDRAHGENPLLDLQSYQDLQSRALSAKIQLSSRERTTIVHTHNGKSIRLELTREKF
                     EEITRHLVEKCKAICEIVMAEAEMQWTEMNKILLVGGMTRMPMVRAMIAGLSSVPLAE
                     DVNPDEAVAVGAAIQAVLSLLNEEDATGEKMLPAETRQQFSSRDGGLIQVRNITSHTL
                     GVVLWDETQLEEYVFPMIKKMTPMPAEAKNSFGTATANMQRAIVRIVEGESTLPAECT
                     PLGICDVALPPFLPKGSPVELTYEYNANQVLEVEVEAAGNCASVSIERKTGLTAEELD
                     RATVALGQLIVA"
     gene            complement(5775..6458)
                     /gene="grpE"
                     /locus_tag="VNW28_07100"
     CDS             complement(5775..6458)
                     /gene="grpE"
                     /locus_tag="VNW28_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXA09733.1"
                     /translation="MSQAFEIEADFAVVMQQLSAQAATSPSAQEDARVPSASGGEISE
                     LTRAVQFLSRRIEVLEGSFSRKLEALQFQKLEEQLAAIRDTETVNQKLFDSLHKELIS
                     YRDNFVRESLQKPFIRDLLILFDDLSAIVGQFGDCPPGTDARGGSGQSHDNLKNTLHF
                     LLEILHRLEVNEIEQKETVDRTLHRVTSYEAAESPEEDGRIVRRVRTGFTWHDRILRP
                     EEVIAKRFQ"
     gene            complement(6466..7989)
                     /gene="cobA"
                     /locus_tag="VNW28_07105"
     CDS             complement(6466..7989)
                     /gene="cobA"
                     /locus_tag="VNW28_07105"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HXA09734.1"
                     /translation="MADKKKLGRCYLVGAGPGDLGLVTLRAREVIERAEVVVYDYLCN
                     PEMLQWAPEAVEIIYAGKQAGAHTLEQDEINQLLVEKARAGKQVVRLKGGDPFLFGRG
                     GEEAQALAAASIPFEVVPGVTSAIAAPAYAGIPVTHRGLASHVTFFTGHEDPEKSESS
                     IDFAALAQLGGTQVMLMGVERIEGIAQQMMSHGVRPDMPVALVRWGTTGRQQVVRGTL
                     QNIAQRVAEEGFAPPGVAVFGEVVSLGKELRWQEERPLSGKRIVVTRTRKQAGALSKG
                     LRNLGAEVIELPTIRIDPPSDLRGFAELVQDAHSYDWIVFTSANGVSAFFEMFYKLYD
                     DAREIGGARIAAIGPATAQRVREFHLKVDLQPEEFVAEAVVAAFKKEGDMENFRILIA
                     RAEEARDLLPKKLVALGAIVDVAFTYKTVPETSDRTAARSRLAAEGADMITFTSSSTV
                     ENFLALGLPWPQGMQVASIGPITSQTASDHGLTVEVEAARHDIPGLTEAICRYFKKH"
     gene            complement(7979..8884)
                     /gene="hemC"
                     /locus_tag="VNW28_07110"
     CDS             complement(7979..8884)
                     /gene="hemC"
                     /locus_tag="VNW28_07110"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HXA09735.1"
                     /translation="MGAPGRIILGSRGSDLALAQTRLVERALQAAWPELEVAIEVIRT
                     RGDEEPKTELLADRKAGRKGMFTREIEQALLVRRIDVAVHSAKDLPSEPREELELGAT
                     PPRGAAEDVLITKTGDGLATIPAGSVIATGSVRRQHQLVWKRPDLQTADLRGNVPTRL
                     RKLRENESWSGVILARVGLERLGLAVQGEILSPDDFVPAGGQGVIALQVRRADAKTRR
                     WIDPVNDRATLTCLRAEREFLRLLGGDCDSPVGVQALLNEGELVLRAQIFEGQTAAPR
                     VGTTTGSAADPEAIAAQLMKQLYGG"
     gene            complement(8885..9952)
                     /gene="hemA"
                     /locus_tag="VNW28_07115"
     CDS             complement(8885..9952)
                     /gene="hemA"
                     /locus_tag="VNW28_07115"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HXA09736.1"
                     /translation="MKKLFCVGLSHHTATVETRERFVGHAAVDRKLREEAGCTEALLL
                     TTCNRVEVYAAAEVPVATERIVRCLLGPGMTPAQDETEFFYRHEAQQCVQHLFRVASG
                     LDSMVIGETEILGQAKKAYAAARESGAAGPYLHRLFQSAFRVAKQVRTRTDITRGAVS
                     VGSVAVDLAEQIFGNFETRKVLLLGAGAASERTARALLKRGVRDIRVTNRSLDRAESL
                     AALICGRAVAMEEWETHCREVDILISSTAAGTPIVTRENLAPLLHDRLDRPLFIIDIA
                     VPRDVAPDVNEMEGVFLYDIDSLQSIAQQSLALRREQVAAGEEIIAGHVDDFSGWFVS
                     AGERMRRAAELPEASLRPSES"
     gene            complement(9949..10740)
                     /gene="ccsA"
                     /locus_tag="VNW28_07120"
     CDS             complement(9949..10740)
                     /gene="ccsA"
                     /locus_tag="VNW28_07120"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXA09737.1"
                     /translation="MDRTLLIVSTLCFFFAMVRTAVAIKARTYHAVGFNFFAIALGFV
                     FQTAFLSVRGHALGRCPLTNLFEVFVFLAWSVSVIYLLIGPAYRLSLMGAFTAPLVFG
                     LQAFALLVPIDRPHPMPVAPTPCLELHASVSLIAYGAFALACVAGLMYLLQERQLKTR
                     QLHSMFYHFPPLTDLFAAITRLLWLGFILLTVGIAAGFFTGQPLPYVKIAWSLGVWIF
                     YAAILLARHLGTTAPRRIAALCVVAFSAALTLLWGITFLSSTHTL"
     gene            10831..11034
                     /locus_tag="VNW28_07125"
     CDS             10831..11034
                     /locus_tag="VNW28_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09738.1"
                     /translation="MKTLARRSSGNKNTADEVEGLRRVLDQSYAQLRERARAEWQRKH
                     SDLDPSDACVRVCIRGNGQSVLT"
     gene            11248..11778
                     /locus_tag="VNW28_07130"
     CDS             11248..11778
                     /locus_tag="VNW28_07130"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF002636.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HXA09739.1"
                     /translation="MVADSIARLRAGIRDVPNFPKHGIVFKDITPILVNARLFQESID
                     VFLERCRTLPIDKVVGIDARGFLFGSAVAYALGVGFVPIRKKGKLPFKTASAAYSLEY
                     GDAEMEMHVDSIEPGEKIVLIDDLLATGGTSAAAAALIQKAGGELLEAQFLIELEFLH
                     GRVKLDPTPVVTFLKF"
     gene            complement(11795..12196)
                     /locus_tag="VNW28_07135"
     CDS             complement(11795..12196)
                     /locus_tag="VNW28_07135"
                     /inference="COORDINATES: protein motif:HMM:NF042458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6572 domain-containing protein"
                     /protein_id="HXA09740.1"
                     /translation="MSSGPAADPAAAPPGGLEQTGVIDFFAHEAKTDEVLLVMFEPRP
                     WDGSDEQLFQLQEKFNAYVSFLLDGELAEAHPELSDKNARIELRCAEMPLGRALDLLN
                     AIHDQLALQEIQVEVIVEDRSGRCGAGCACG"
     gene            12290..13153
                     /locus_tag="VNW28_07140"
     CDS             12290..13153
                     /locus_tag="VNW28_07140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09741.1"
                     /translation="MRKSPLALVALAIAGTLPASGQFTPGFVTNSGYWNDGKAEFNIY
                     DARIVRDGQPRQTEVLHILVREPFDLRQMVKPDNWQRPNVIQVLKMNQILHIPTGLYV
                     FQQMHSNFWRADNARLAKFSLTSNDSCGNTYKEARRGGEVFTCQWHTYWDGMAEGREK
                     VRLPPNGFFYDELPLRVRTIDFSKGSGDFAIQLAPTIINSKKDNLVWKPAKVHFESTN
                     RAIYVSVEHAAGTDRFMLGRGFPHLLREWAAADGSRLTLKRSLKIDYWNYHNLGDRKR
                     ALLNSLPARPD"
     gene            13217..13639
                     /gene="queF"
                     /locus_tag="VNW28_07145"
     CDS             13217..13639
                     /gene="queF"
                     /locus_tag="VNW28_07145"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HXA09742.1"
                     /translation="MAKKTVNKRLTLLGRSETRIPAAPSAATLETFANPARRPYWIRF
                     HSADFTSLCPITGQADFATITIDYLPGARCLETKSLKFYLASYRNERAFNESVANRIL
                     DDLVRACAPRKMTIEAEFAARGGITLTITASHPDVSAR"
     gene            13636..14319
                     /gene="queC"
                     /locus_tag="VNW28_07150"
     CDS             13636..14319
                     /gene="queC"
                     /locus_tag="VNW28_07150"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HXA09743.1"
                     /translation="MKRAVVLLSGGLDSATVLALCRAENYAAYALSFRYGQRHEREIA
                     AARRIAKSLGTTEHRIAEIDLRLFGGSALTADLAVPKARSEVEMAAGVPITYVPARNT
                     IFLSYALAWAEVLGATDIYLGVNAVDYSGYPDCRPEFIRAFERLANLATRAGVEGARF
                     EIHAPLLALSKAQIIQRGSELGVDFSLTHSCYDPTPDGLACGECDSCQLRRRGFREAG
                     REDSIRYAR"
     gene            14316..14516
                     /locus_tag="VNW28_07155"
     CDS             14316..14516
                     /locus_tag="VNW28_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09744.1"
                     /translation="MNLRARLGMFVLTRQEQRAIAFIVLMLVLGLATRHYRHRHPKSA
                     NSPNETVRAVPTPTLAPAKNPP"
     gene            complement(14553..15626)
                     /locus_tag="VNW28_07160"
     CDS             complement(14553..15626)
                     /locus_tag="VNW28_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HXA09745.1"
                     /translation="MTRNNRRVILRLTNRLDVGAITLLLLISLGPTQGVGAEEPSLGA
                     KIRKFLATPTPTPSHRPHKRVTKKSSPTPSPSPSAAPKSTAKRLHKSFPTPTPEEEET
                     PKPKRKNPSPSPSPSASPSPRKTPTPTPDEEETPKPKRKKPSPSPSPSASPSPRKTPT
                     PKPEESPSPEKRVAAVSPDEIEGYDKNPAAVQKLLGDALDLTKRNLGYLYGSADPAGG
                     GMDCSGFIYYLLRENGVPDVPRDASEQYVWVRKAGNFRAVLSRDLDSFELDELKPGDL
                     LFWTGTYSVERDPPVTHTMIYLGREKESGKPIMIGASDGRTFHGKERFGVSVFDFKTA
                     PSKDGPSSQGQPRFVGYGRIPGL"
     gene            complement(15716..17059)
                     /locus_tag="VNW28_07165"
     CDS             complement(15716..17059)
                     /locus_tag="VNW28_07165"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HXA09746.1"
                     /translation="MRDSHLQDFYAFLRFPSVSTDDAYAEKLGECARWLVEKLNGIGL
                     EAQLVSTPGHPVVWAKNKHQPGRKTVMLYGHYDVQPPDPLELWESPPFDPVLKDGYVY
                     ARGATDNKGQILAHILGIEEVMAEHGDLPVNLHLIIEGEEEIGSNNLPGFLQEHRAAL
                     KCDVAVVSDTGMIARGVPTLSYGLRGVTACEVKVTGAKMDLHSGVFGGAVANSVTALA
                     RLLATLHDDSGHVAINGFYDDVKPLEEWEREAWRKLPINADKEILAETGAPALFGENG
                     FSTLERLWARPTAEINGIGGGYQGKGTKTVIASHAMVKLTFRLVPNQNGDAILKLAHQ
                     HLEKHLPPGVTMEITDGHSGPWYLTDPHSQFGQAAQYALKEAFKAEVALIREGGSIPI
                     VSQFRDILGVETLLLGLALPNCRAHSPNENFPLENLEAGIKMNKAILQEIGTGGR"
     gene            17137..18453
                     /locus_tag="VNW28_07170"
     CDS             17137..18453
                     /locus_tag="VNW28_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXA09747.1"
                     /translation="MSAPVEPAVEKQLIRALGVPTLAANIVNSTVGAGIFALPALIAA
                     QLGTAAPLAFLACALAMVVFVTSFALAGSRVSLTGGMYAYVEVAFGRFGGFIAGVLYF
                     LTAILATSGIVSLLADTVGTISPLFANKPAQFLVVLSVLGSLAWINVRGVQSGARAVW
                     IVTIVKLIPLFIFVGAGIFFVRQADVLTMSWPGASALGRSVLILLFAFVGIEVALIPS
                     GEVKNPTRTVPRAIYLALAVTTALYLLIQLVAQGILGGSLGKFSAAPLAEAAARFLGG
                     PGRSLLLTGASISAFGFITSDILSSPRILFAFGRDRFLPQAFAHVHRRFHTPDVAIIV
                     YCTICAILSLSSSFQQLAILSNVAVLALYFLSCAAAVQLIRRDVRADGPPFAFRGAWI
                     MPCVGVVISLLILAQAKREELTLTAVVVVVAAIVFAVQRTVRKPAC"
     gene            18565..22278
                     /gene="smc"
                     /locus_tag="VNW28_07175"
     CDS             18565..22278
                     /gene="smc"
                     /locus_tag="VNW28_07175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HXA09748.1"
                     /translation="MHLQSLELFGFKSFADKTVFNFHEGVTAVVGPNGCGKSNVLDAV
                     RWVLGEQSAKALRGGEMADVIFSGTDSRKPFGYAEVSLTFTDCATELGVDWHDVRVTR
                     RVYRDGISEYLLNKTVCRLKDIQSLFADTGVGRSAYSIMEQGRIDQILSSRPEDRRAV
                     FEEAAGITKYKAQKREALRKLEATEANLLRLGDIIKEVKRQIGSLQRQAGKARRYQAV
                     LGDLQVLDTHHSRRQLDSLEAELERSREAIGRFEEQQHTTERAIEEHENEVEAQRGRL
                     EEVDARINDARAAVQGLQNQLTAHRNRIDFNRERAQELSALIERYELDVQTAETKRAQ
                     QETEIHDTDVLLAETERLLAAKQTELEQLSARAGEVRRQREGRERELQTLELAILKAE
                     NRLADLETEISGTIDRRTATEPRVAELAASLGELGGARDLVATQLAATRARADEQQEA
                     FQDLAAQVPAAEEELGRQQQRLREAETALMASDRALAERQSRLEILQQLNAEGEGLTE
                     GSQAVLRGLDDPARILPALNGALASLIEVEEKFIPAVEAALGRNLHAIVLQNSALATE
                     IIAAVTARKLGQTALIAPELSVGTPVDARILPPGALAWATEKIHASDHLIPLVRHLLG
                     EVALVADLEQAQKLKKKFPALAFATLAGELVSTQGVIFGGSVDGENSSLFARKAQIAA
                     LAAEHRALLEEQQNLRAQHDEIQRELDAAAERLDEIREQHQAARGDHSATALQVSALE
                     REWHEAGRKVDALGWEKTTLHQQLTSASERVAQLESEATGQREFLVAGRERQVALQTA
                     AEAARLRDEQLVEQLSELRLAGATERQRHEGLRSQRQPMAVRQAELAELIAARRADIA
                     NYQARREQQSTESAAAERGIEENSAQLSGAESAVAEIAETRAARLLEVNTLETDLRAR
                     RKALSDLHDARGKEEVRQTQMQLKIESIAEHVMRRYQVDLREFAPDSYAFTKTFRAQL
                     KQRGKSEPDDTEAFDEAPLREIIDQLTRQLDAMGPVNLDAVHEYDALEERHAFLETQN
                     NDLTAARRELLDVITRINQTTEKLFAETFAQVRINFREMFAELFGGGRADLTLLDEND
                     ALNCGIDIIAKPPGKQLQSVSLLSGGERTMTAVALLFAIYMVRPSPFCILDEMDAPLD
                     ESNINRFIRVLERFVAQSQFIIITHNKRTIAKADILYGVTMEERGISKLVGMRLTGGN
                     PEEETRPMRAEPRTEQRALELDDAPAENVALTR"
     gene            complement(22474..23790)
                     /locus_tag="VNW28_07180"
     CDS             complement(22474..23790)
                     /locus_tag="VNW28_07180"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HXA09749.1"
                     /translation="MKIVFLLLTACFLSSAPLIVIAQPTSISLTTAPETQKPLDPAAA
                     TRAWLATVPAEKRARSDAYFEGGYWLILWNFLLGAAISVFLLSSGLSARLRAFAERAT
                     RFRSLQIALYAVPYVLLVFVFSFPLGVYSEYFREHAYGLATQTFGPWFREQLIDLAIA
                     IIATTIALLVLYAVFRCARRTWWLWGTGAGVLFLAIATLIAPVFVAPLFNTYKPISDP
                     KISQPILAMASANEIPVRQVFEVDASRQSNRVSANVSGLFGTARISLNDNLLKQCTLP
                     EIRAVMAHEMGHYVLNHSFKLILYFALFIFGGFAVARAIFERAVRRWGGRWGVRDIAD
                     PAGFPLLVLIFSTFFFLATPLTNTAGRVTEREADAFGINTAREADGMAKAALKLGVYR
                     KLDPGPVEEFIFFDHPSGRARIRMAMDWKAANLPAGDAGAALSTAR"
     gene            23937..24251
                     /locus_tag="VNW28_07185"
     CDS             23937..24251
                     /locus_tag="VNW28_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09750.1"
                     /translation="MTDGVAANPPPANAVYGAYPTNYQDIVTNWLNAALVDPKSVKVK
                     WLGEPRPGELPVGKGQKVTGFLVDFSVNARNIFGAYTGSQKHTALIRDGQVVTATGFV
                     YH"
     gene            24255..24557
                     /locus_tag="VNW28_07190"
     CDS             24255..24557
                     /locus_tag="VNW28_07190"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HXA09751.1"
                     /translation="MEPNEKRGYVIVEIEVTDPARYEEYRRQAEATVVSQGGKYVVRG
                     GGLQTLEGDWQPKRLVVLEFPSVAQARAWWSCEEYRAPKKIRQQSAVTRMILVEGV"
     gene            complement(24559..27384)
                     /locus_tag="VNW28_07195"
     CDS             complement(24559..27384)
                     /locus_tag="VNW28_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09752.1"
                     /translation="MSFRKRLFVFLLVVLLCGALDALFAPVVVAHGVRLWFWWVARTQ
                     KLSVELGEVDAPFLREVTIRNLRVARAGDASREVSFRAARVVVDLNFRGWIFSRRARL
                     LRSINLDRPTAGIRLPAGAVSARKLDWRTLARLLPNNFRIEHLDFDLAVGETALSLRD
                     VFLTASAIESGKFLARQVTVSSPVLRQTFGNLRGATSWEGDRLTIAGIPLVEGLDLEG
                     LTIDLSHLGRRRLGLDLHLDTYGGTLRASFQGRANATFEVDVAGSAANLSLAQISKGM
                     GFLEPITGAVRASKFTFRGNPGEFLDATASLWIELTDFAWRARRADNVMLGATFYDRR
                     LEVDQLYVRQRRNELTVNGELPWPKRASDWARLPFRGQLNAFIPDLNGFAQFLGATTG
                     DFTGALSAEGELDSLAPEAHGRLALHGEGVTFRGVALDSLGAALQLRGTEATLENLEV
                     RHAEDFTRAQGAIDLASPHRFSGRLTGAINDLGAYAPLLPAAWRSSKIGGGVTFDWSG
                     DGTLAAHSGTMQFFAHGLQLPVAPLRLPLDVTLVGTYSPQDVFFRTFKLASARVSLGS
                     FLMLGSNFVELQALELTLDGMPRVSGTLFLPLSFERWRTSGSLLAALDEGQKFDVDLA
                     INHLDVAKLASVLGEDSAATGVVDGKLAAFGPLRSLQVTTSWRLENLGPATSSNSIDL
                     EGHYADGRAEVKTTAIFGVSDPVMLRAWLPLQLGKDRLAAGTVIEPAGQFSVAIDCPA
                     LFLDTLPNELRFGAESGLVTGGIAFFNTLELPSINGAAQILEARFKPPAPWPELTGLE
                     AQLQFKNTEAVIAPLRFEINSTPIGLRGRLTTTPVAFGLTLTPVDDALQLVGLPGGGA
                     NLSTVQLLGKGTSEDLPSLREAFVRGGIGSGALSLTITSEGPASDDAVSQATFYVHPR
                     APDARPLLLRMVSPGPPTVLQLAAPKP"
     gene            complement(27413..28108)
                     /locus_tag="VNW28_07200"
     CDS             complement(27413..28108)
                     /locus_tag="VNW28_07200"
                     /inference="COORDINATES: protein motif:HMM:NF025380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HXA09753.1"
                     /translation="MELQTSRPAAEKLPRRLGCPGAAVLIVFVLALLAAWLFSRVQKW
                     PARAVGQSVVELERLGRDARDTFVTLAHLQPRVIVNNRVYLEQTTTVAELSVLARRVE
                     VEHEMLHSWAGSTKRIKLHGTFVVKAGFDLRKKFSVNLRPEEILIELPHAQILSVEEQ
                     QIEVLALENGLWNRIVPDDIQTELALLPQLAREKSAALPAEAEQVFTRELREKFHPPQ
                     PVRAIFPAPSPGG"
     gene            28274..29125
                     /locus_tag="VNW28_07205"
     CDS             28274..29125
                     /locus_tag="VNW28_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HXA09754.1"
                     /translation="MKIGVRYETAYRYTAPVSFMTHEIRLFPRIDVFTRMHRMKFETN
                     AGADVRFGRDVFDNCVAACSFAAPATELRFQLELDLELLEKNPFDFLLTTDALTLPFS
                     YPSELHRVLAPFRESDAGRLLEIPRWERPQTGAPRPTVATLVELNERLHEGVGYERRE
                     EGPARLPAETLRIGRGACRDVALLLAAILRQCGLAARLTSGYLREAESDLRRAEGSLH
                     AWTEVYLPGAGWVGMDPTNGVFANHNFIPAAVGITPGDVTPIDGSFFPRQAGSAQMTS
                     RLELLSL"
     gene            29137..30192
                     /gene="recF"
                     /locus_tag="VNW28_07210"
     CDS             29137..30192
                     /gene="recF"
                     /locus_tag="VNW28_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HXA09755.1"
                     /translation="MLRDLQLRDFRCFAAIAVEFSPGFNFIVGANGQGKTTLLEAACV
                     LLRLQSQRSSSLAPTVRAGGRSFIVSGHAGEHLLQFYYSALRRKVAFDGVEQHDQAEY
                     LRLFRVVSFASHDLELIRGPGEMRRRYLDFIGTQTEARYRPVLRAYERALRSRNVLLK
                     SPHARPRELAAYTEPLVRAGTSLREMRATLVRRLAPCAEISHRAISGGHENLSLEYAP
                     GSEEDFAAQLARTRTEEGRLRLTIVGPHRDDLRLQVEAMPAAHFASEGQQRTIALALK
                     LAQAQVFAEDAATPPLLLVDDIFGELDAARRNALLAHLPNESQKLVTATALDWRAGDW
                     AGPVLELADGQIRRRDK"
     gene            30341..30835
                     /locus_tag="VNW28_07215"
     CDS             30341..30835
                     /locus_tag="VNW28_07215"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HXA09756.1"
                     /translation="MLDPNFRRCVLFISAHDENEGAVGVIINRPLDKHVSELVTDPTP
                     AALADVPVFLGGPVATNQLMFAAFDWKGRDSLSLDQNVALEEAHQLAERDRSAVRAFV
                     GYAGWTAGQLENELRQNAWVLQAPNRAALTPERLPKLWFEIMRGLGPWFKMLSAAPDD
                     PSLN"
     gene            30872..31999
                     /gene="ald"
                     /locus_tag="VNW28_07220"
     CDS             30872..31999
                     /gene="ald"
                     /locus_tag="VNW28_07220"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HXA09757.1"
                     /translation="MIIGIPKEIKQQENRVSLLPSAAYQLTKRGHTVLVEKSAGVGTG
                     YPDSDYAASGATIIDQARDLFQQAEMIVKVKEPLPAEYGLLRKGQILFTYLHLAASRP
                     LTEALLKSGVTGVAYETIQVGNRLPLLEPMSEIAGRMSVVMGAYFLAKHNGGNGVLLG
                     GVPGVLPGKVAVLGGGTSGVNAARMATGLGADVTILEVDLERMRFLDMTMHSANTLYS
                     SEAHLQELMPAVDLLIGAVLVPGAKAPKLITRDMLREMRPGSVVVDIAIDQGGCVETS
                     RPTTHLNPVYVEEGVIHYCVANMPAAYARTATQALTNITYRYVELLADLGLKEACERQ
                     PALTGGINTMDGRLTHRAVAEAHGMEYEPPFQPAPGGAFPS"
     gene            complement(32917..34773)
                     /locus_tag="VNW28_07225"
     CDS             complement(32917..34773)
                     /locus_tag="VNW28_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXA09758.1"
                     /translation="MTRVLGSAVQNFPIAPSMKRRKRKINPSIEDYAFLSDTQSGALV
                     SRDGSVDWLCLPRFDSGACFAALLGKKENGCWQFKPKGRIKKCARRYRGDSLILETEV
                     ETAEGAVRFIDFMPPRGTNPDIVRIIEGLKGRVTINMELIIRFDYGSIVPWVRQRHGG
                     LEAVAGPDALILRTPIPTRGQDLKTVAKFTVAQGDCVPFVLTWYASHELPPKEVDPGH
                     ALQETEIYWLQWAERYGRSGRWRDAVMRSLITLKGLTYAPTGGIVAALTTSLPEKIGG
                     VRNWDYRYCWLRDATFTLTALLGAGYEEEAKAWREWLLRAIAGSPAQLQIMYGVRGER
                     RLDEYELPWLDGYEKSRPVRVGNAASGQFQLDVFGEVVDAMFQAHRCGLEMNEADWRL
                     QVALLRFLETKWHEPDEGIWEVRGPRRQFTHSKVMAWVAFDRAVKLVEQYHCAPGQSL
                     ERWRKLRDKIHRSVCRFGYNKKKNAFTQSYGADVMDASILMLPLVGFLPPNDERVLGT
                     IVAVERELLENGLVLRYRPGEEKVDGLPGPEGVFLPCSFWLVDCYYLTGRGKDARLLF
                     ERLLDLRNDLGLLSEEYTPRKKRLLGNFPQAFSHVALVNSALRLCREGESRL"
     gene            complement(34800..35318)
                     /locus_tag="VNW28_07230"
     CDS             complement(34800..35318)
                     /locus_tag="VNW28_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXA09759.1"
                     /translation="MSGIKKSDFSIRLSARADVPAILALIRELAEYERAPQDVVATEA
                     ALEEALFGAHPAAEVLLGEEGGVPVCFALFFHNFSTWLGRRGLYLEDLFVRPEVRGRG
                     YGRALLVRLARIARERGCGRMEWAVLDWNEPAIQFYRKLGARPNEEWTIFRLTRDGIA
                     QLAQEPGGVVCE"
     gene            complement(35371..36495)
                     /locus_tag="VNW28_07235"
     CDS             complement(35371..36495)
                     /locus_tag="VNW28_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HXA09760.1"
                     /translation="MSTTARKLSQERKKIASLEIITDPQEAAEEAGLRYVSDESPGYT
                     RRRRGRTFLYFDTEGREIHDEARVMRLNRLAIPPAYTDVWICPSPNGHLQATGRDERG
                     RKQYRYHDRWREERDENKYEKMIVFGQALPGIRRRINRDLKRRGLPREKVLATVVQLL
                     ERSLIRIGNEAYAKENKSFGLTTMRNRHVEVKGAKVRFRFRGKSGQAHDIDTEDRRVA
                     RIIKKLQELPGQEIFQCLDAKGGQHQVTSDEVNQYLRETTGEEFTAKDFRTWAGTIMA
                     ATVLQAQEPFENKSQAKKNIKNAIGAVARVLGNTPAVCRKCYVHPAVLETYLDGNLIA
                     GLRKRREETLADSFGDLRAEEAAVLAFLQEKLAKKAEAWP"
     gene            36543..37043
                     /locus_tag="VNW28_07240"
     CDS             36543..37043
                     /locus_tag="VNW28_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXA09761.1"
                     /translation="MKILETERLLLYPLTTKDADFMLMLMNEPGFIKYVADRGLRTSA
                     DAADYISQKIIPSYARFGFGLYRVDLKESKTAIGICGLVKRDTLEEVDIGFSILQRFC
                     GKGYAYEAAAAVMRYGRNVLGLPRIVGVTAPDNRASIHLLEKLGLKRQRKIHLPGYGP
                     ESLLFG"
     gene            37178..38977
                     /locus_tag="VNW28_07245"
     CDS             37178..38977
                     /locus_tag="VNW28_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXA09762.1"
                     /translation="MNHQAFFPFAKLNLALIASLFFLAPVPRAVRAETASQESEPTEV
                     YATASDGTILHWVVYTPAGAGPWPAVLVIHGGGFKGGTPTSSPESVSCGRDLAAAGFI
                     AFSIEYRLAPPGSLLGQVTDGRFPDQPDDVKLAVLAARNDARCNGQVGSVGGSAGGYE
                     TAFVAGAGTIGQDRIDVGVSLSGAYDLSDFSPDPDIQVFADDVTNYVGCTTLDTGLLR
                     AASPAYLADSNTAPLFIVHTEQDPMPFSQVADMTTQLDALGVTNYQALTLAGNQHSFS
                     YWPTVKDQAIGFLGSWFAGVPPPPLPTPTPTATPTATPTPTPSPSPTATPDQTASPAP
                     PPVIAPTPTKMLLNVSTRVRVESGSSVMIGGFIITGDVAKKVAMRAIGPSLADAGVSG
                     VLADPVLELYDSTGSLIAQNDNCSSLPPDSIPADLKPTDGHESFIAVTLPPGSYTGVL
                     RGANGASGIGLFELYDLDPASSRISNISTRGDVGTGSDVMIGGFIIGGAEPTKVIVRA
                     IGPSLVAENISDALPDPLLELYDSNGSLIFTNDNWRTTQEQQIIDSGVPPSDDRESAI
                     VATLLPGSYTATVRDAGLATGIALIEVYNQESN"
     gene            complement(39048..39488)
                     /locus_tag="VNW28_07250"
     CDS             complement(39048..39488)
                     /locus_tag="VNW28_07250"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HXA09763.1"
                     /translation="MAEVQTTTQSGELTQRFIEFVMMQAQNAALFLGQIPNPQTGQAE
                     VNLEVARLFIDQLAMIQEKTRGNLTSEESTVLRNALSSLQMAFVEASQSGRPGRTPAP
                     ASGAAPDVEPGAPAAGSPPGEAPPSAVTEPETESKKKFTKTYGA"
     gene            complement(39526..>40662)
                     /locus_tag="VNW28_07255"
     CDS             complement(39526..>40662)
                     /locus_tag="VNW28_07255"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HXA09764.1"
                     /translation="GLLAETRATSGLTRLMAKVLVKGTKTRTAEQIADTIEAVGGTLG
                     SDAGNNSFNISLEVTKPDLRLGAEVLGDILLNATMPEKAVAREKEVQLAGLRDDEEQL
                     TTVARNILRAALFGQHPYALRAKGTPEAVAQLTQKDLLAFRTRYLVAKNGVISVFGSV
                     KAAEVKQLFEQILSAMPAGELALTDAPSPPRLAKTIAVEEVKEKAQGVLMVGYRGAGL
                     FSPDRYALELIDEASSDLGSRFFVRIREEMGLAYYVGTSQMQGLVPGLFAFYLGTDPA
                     KLKPVTAALLDEIGKLAAAGLTNEELTRAKKKLIGQQRIANQSNDSFAYMVALDELYG
                     LGFDHYRKLEDEVGLVTPDDVQRVAAKYFQQPYALATVRPASHI"
BASE COUNT         7900 a        12808 c        12154 g         7800 t
ORIGIN      
        1 agcccagtcg gcacggaccg aatcctggtg caaatccccg ggctggatcc ggccaagatc
       61 atcgaagcgc gccagcaact ctcccgggtc gcgaagctcg agttccggct ggtttatccg
      121 gataatggcg aacgcctgcg cgcgatcgac gccggaacgg aagttatccc gccggaatac
      181 cggattgaga cttacaagat gcaacaggcc gaaggccaga agccggtcga agagcgcctt
      241 ctggtgaaga aaaaggccga cctcggcggc gaccgcgtga ccgaatccaa cgcctactac
      301 gggaacgaag ggtggaccgt gcagttgaag tttgacagcg aaggcgcgaa gaaattcggc
      361 cagatcacgg aggcgaatgt cgggcaccgc ttcgccattg tgctcgatgg cgtgatccaa
      421 tcggcgccga gtattcggac cgccatctat ggtggtgacg ccatcatcac cggccatttc
      481 ggcgagcagg aagcgcgcgg tcttgccagc gtgctcgaga atccactcca gacgccggtc
      541 agcgtggagg aagagcgcag cgtctcgccg acgctcgggc tcgattcgat tcgggctagt
      601 atttttgccg gtctcgtcgg cctcgcgatc acgctcgtgc tcgtgatggc ttactacaaa
      661 ttcgccggcc tgatcgcgaa cctcgttttg atcgtgaaca tcatcctcct gatgggcgcg
      721 ctgacgatgt tcaacttcgt cctcactctt cccggcatcg ccggcatcat cctgacgatc
      781 ggcctcgcgg tcgacgcgag cgtgctgatt tatgagcgat tgcgggaaga actggccctc
      841 ggcaagtcgc tcaagatcgc ggtccagacg gcttacgaga aggcattctc gtccattctc
      901 gacgccaacg tcacgaccct aatcacggcc gcgattctct tctggaaagc gagcggcccg
      961 gtcaagggtt tcgcgatctc gctgacgctc ggcattctcg cctcgctctt cacggcgctg
     1021 atcgtcggcc ggaatctttt cagctggttt gtcgacaccg gaaaagtgaa gcgcgtttcg
     1081 atgcttcacc tcatctcgtc gaagaatatc aactttctcg gcaaaggttt cattgccacg
     1141 atgtgctcgc tcgccatgct gatcgcgggc gggaccgctt tcgtcatacg cggcgatcgc
     1201 aacttcgggg tcgacttcag gggcggcgac ctgctctcgc tgagcgcggc caagacggtg
     1261 caggtcgccc agattcgcga ggcactgaaa ccgatccacc tcgacaacga accgattcag
     1321 caatcgctcg agggcaaccg caacttcatc acgattcgga gcccgattaa taccagcgac
     1381 caggttgcca aagcgattaa ggaatacatc ccgaacgggg aattcaaggt ggagcgcgag
     1441 gaacgcgtcg gcgccctggt cggtggcgag ctggcgagaa attcgctttg ggcgctgggc
     1501 ctcggcattt ttgggatcct catttacgtc actttccggt tcgagctttc cttcgctgtg
     1561 ggtgccatcg tcgcgctgct ccacgacgtc ctgatgacgg tgggagtctt ttcgctgatg
     1621 ggccgggagt tgactttgac gatggtcggc gccgtcctca cgatcgccgg ttattcgatt
     1681 aacgatacga tcgtcgttta cgatcgaatt cgcgaaggct tggccagtgg gcgacgcggc
     1741 tcgatcgagc aaatcatgaa cgagagcatc aaccagacgc tcagtcgcac catccttaca
     1801 agcaccgtga cgctaatccc gatcctgtgt ctcttcttat ttggcggcgc ggtgctacgc
     1861 gacttttcgc tcgcgatcat catcggcgtg gcggtcggaa cctactcgtc ggttttcatc
     1921 gcttcgccga tcgtcctttg gtggacgcgc gcccgcggcg gcagtgcgag cagtttgcgc
     1981 cgcgaagtga ctcaaaaaag cgcggtcacc agtccctcgt cggcctccta aacccggagt
     2041 tgacgctgcg ggaaccgagc cttaggatcg cacgccaatc agcggggaag ctttggattt
     2101 aacaggatta ggagaaataa tcgcatgcca gaagtgatcg ttcgtaaggg tgagccggtg
     2161 gaccgagcct tgaagcggct taagaacaag ctggacgcgg aaggcatttt ggaagaagtg
     2221 cgccggctgc gcgccttcga aacgccctca caaaagaccc gtcgcaaggc gaaagccaat
     2281 gccaagcgcg ggcgcgccaa gttccgtttc aatccatcgt aaacagctcg ccgatagact
     2341 tctagacgct tcgatctgcg aagcgcaagg gtggccgtcg ctgctcttgt tggccccgtc
     2401 cattcgacgc ttactcgcgg cagtcctcga gcgattgggg tcgggtcggc aatcgaacgt
     2461 tttcccccta gtttcgcagc gcgaaacgtc tacatatcgc actcgcagac aatccgaaag
     2521 cccaggtcgt tgcacgagta attgggggcg ttggcacccc gcgtggtcgc gcgcaggctg
     2581 tggaagcgtg atttccagct tccgccccgg taaatccgct ttgaacctga gaccgctccc
     2641 cggggattga tttttggagt tccgcgatag ccttccatga aatcgaggca ccattcccag
     2701 acgttcccag ccatgtcctc tatcccgaaa ggactcgaac ccaagggata ggccccgact
     2761 ggcgaggtct cggcgtggcc gtcgtcgatc tcgcggtcgc tccaggcgaa agtggtgttg
     2821 cgatcggcga aattcgccaa gttgcccctc ccttcgtggt tgccccatgg atatttgcga
     2881 ccgtcgctgc cgcgagcagc gtattcccat tcagcttccg tcggcagccg gtagcggcga
     2941 cgttcccgca cgctaagcca ttggcaaaat ttcatcgcgt cgccgctgct cacataaacc
     3001 accggatgcc ggtcacctgt gcgggccata cgtttgcgcg cgtgactcgg atcaaacatc
     3061 tcgtattgcg cgttggtgat gagatgccgg gagagataat agcgggtcag cgtcacctgc
     3121 gttatcgggc gctcgttagg cgcagcatcg gcggcttcgc tgcccatttt gaagacaccc
     3181 gacggcacaa ggaatatttc agcgccgatc gtgttaacga aattggaata tttggcccgc
     3241 tccgcgttcg gatcgatgac ggctggcgcg gacagagtcg gcgccgttga ggccgggact
     3301 ggctccggtt tcggagcgac gactcccggg accgccttgg tcgctggcaa agcggcctgc
     3361 ccttcgattt cctcgaggta acgatcgacc atgtcctcgg cgtcgcctgg ttcgatgccg
     3421 agattttccg ccatgttcac gaaggcgtcg cgttgatcgt ccgtcatctc atcggagccc
     3481 agaccactga gccggagcat ccgcataaaa tcgtcttccg gattgagcgc cggtcgattg
     3541 ttccctgcgg ccgctgtttc ctccaccgag ggccggctga ggcgcgcccc accgcaggcg
     3601 agcagctcag cctcgatcaa ctccatcatg cgctcgtccg ccaggtcctg ctcccgacca
     3661 aactccaaca aggtttgctc ttcctgcgga gtgagggtct tgtcattcag ggcaaaagcc
     3721 aagtacttgt tgaattcggt gatcgcctgc tcttgcgcct gggcagccag ttcgtcatcc
     3781 accttttgcc ggcgctcggg gttgagcaac tcgacgcggg cttccgtgag gaagcgcgat
     3841 gagtcttcga gcccggagcg caataactgc gcgagcgggt tgctcggttg attcttaagc
     3901 ggcagcttct tttgccacca cagaaccaga tggcgaaagc gctcttcgat cagctcgttg
     3961 ctcggattgg tgctcggatc gaggcagagc cgctcgtaca aatctggcga atcgtaacgg
     4021 ctccagccgt cccacttcct cgggtcatcc ggtaaaggaa tcgggaagct gaccatccgc
     4081 gtgatcgctt aagcgacgat taattggccg agcgcgaccg tcgcacgatc caattcttcc
     4141 gcggtcagac cggtcttccg ttcgatcgag accgaggcac agttgccggc cgcctcgact
     4201 tcgacctcga gcacctgatt ggcgttgtat tcgtaggtca actctaccgg cgagcccttg
     4261 ggcaggaagg gcgggagcgc gacgtcacaa atcccgagcg gcgtgcattc cgccggcaat
     4321 gtgctttcgc cttccacaat ccgaacgatc gcccgctgca tgttggccgt ggcggtaccg
     4381 aacgaattct tcgcctcggc cggcatcgga gtcatcttct tgatcatcgg gaaaacgtat
     4441 tcctccagtt gggtctcatc ccaaagaacg accccgaggg tgtgcgaagt aatgttgcgc
     4501 acttggataa gcccgccgtc acgcgaagag aattgctggc gggtttccgc cggcagcatt
     4561 ttttcgccgg tcgcatcttc ttcgttcagg agtgacagga ccgcttggat ggccgcgccg
     4621 accgcgacgg cttcgtccgg attcacatcc tcggccagcg gaacggaaga gagtccggcg
     4681 atcatcgccc gcaccatcgg catgcgcgtc atgccaccga cgagcagaat cttgttcatt
     4741 tcagtccatt gcatctccgc ctcggccatc acgatttcgc agatcgcctt gcacttctcg
     4801 acgaggtgtc gggtgatctc ttcgaatttt tcccgggtca attccagccg gatgctcttg
     4861 ccattatggg tgtgcacgat cgttgttcgt tcgcggctgg agagctgaat tttcgcggat
     4921 aaggcccggc tttgaaggtc ctgatagctt tgcaggtcga gcaacgggtt ctcaccatgt
     4981 gcgcgatcga attcttccgc gacgtagttg acgatgacat cgtcccaatc cttgccaccg
     5041 agccgatggt cgccattggt ggccaccatc tcgaggcgat gtccctcgat ccgcatgacc
     5101 gtgacgtcga aggtgccgcc gccaagatcg aagacgaaaa cggtttggtc ctcgtccagt
     5161 ttgtcgagac cgtaagcgag cgccgcggcg gttggctcgt tgatgatttg caagacgttg
     5221 aggccggcca gtcgaccggc cgtgattgtc gcggtgcgct cggcatcgtt gaaataggcc
     5281 ggcacggtga tgaccgcatc ggtcaccgga gtcttgaggt atttctcggc atccgatttg
     5341 agctttttca ggatgagcgc cgagagttct tcggccgaat aagcaacccc gctgaactcg
     5401 cgacgaaatt gttccttcgc tttgccgatc tcgcgcttca cgaaatcgac tattttctcc
     5461 ggctccgcga cggtgttatt tttcgcaatc gtccccacaa tcgcattgct cccgtcgaac
     5521 aggacgacgg aaggagtgat tcgttcgctt tcggagttgg gaatgatctg cggttttccg
     5581 taggcgtcga tgtgcgccac ggccgagaag gtggtgccga gatcgatgcc aactgctttg
     5641 cgattactca tattttgaaa gcctcttttg cgctcttttg actggaatag ccactccccc
     5701 aaatgcagtt ttcatgcctc agccgctggt tatcgcagag ggtttcgctc cgcgggcgcc
     5761 catcgcgccc gctgctactg gaatcgcttg gcgatcactt cctccggacg aaggatccga
     5821 tcgtgccagg tgaagccggt cctcacccgc ctaacgatac gcccatcttc ctcagggctt
     5881 tccgccgcct cataactggt gacccgatgg agggtccgat cgaccgtctc tttttgctcg
     5941 atttcattca cctcgaggcg atgcaggatt tcgagcagaa aatgaagggt gtttttcagg
     6001 ttgtcatgtg actgcccgct cccgccgcgc gcgtcggtgc ccggaggaca gtccccgaac
     6061 tgcccgacga tcgcgctgag gtcatcgaac aaaataagga gatcgcgaat gaacggcttc
     6121 tggagggatt cgcggacgaa attatcgcgg tagctgataa gctctttgtg cagcgaatcg
     6181 aacaactttt gattaaccgt ctcggtgtcc cgaatcgcgg cgagctgttc ctcgagtttc
     6241 tggaattgta gcgcctccaa tttccgcgaa aacgagcctt ccagcacttc gatccgccga
     6301 ctcaggaatt gcaccgcgcg ggtcagctcc gaaatctcgc caccggatgc gctggggacc
     6361 cgcgcgtcct cctgggccga cggggaggtc gcggcctgcg cgctcaactg ctgcatcacg
     6421 accgcgaagt cagcctcgat ctcaaatgcc tggctcattc ccgggtcagt gctttttgaa
     6481 atagcggcag atcgcctcgg tcagcccggg aatatcgtgg cgagcggcct ccacctcgac
     6541 ggtcagtccg tggtcgctgg cggtttgcga tgtgattgga ccgatgctcg caacctgcat
     6601 cccctgtggc cagggcaaac caagggcgag aaagttttcg accgtcgatg agcttgtaaa
     6661 ggtgatcata tccgcgcctt ccgccgccaa ccgcgatcgc gctgccgtcc gatcgctcgt
     6721 ctccggcacc gttttgtagg taaatgcaac atcgacgatg gcccccaggg cgacgagctt
     6781 ctttggtagc aggtcccgcg cctcttccgc ccgcgcgatc agaatccgaa agttttccat
     6841 gtcgccctcc ttcttgaatg cggccaccac cgcctcggcc acaaactcct ccggttgcaa
     6901 atccactttc aagtgaaact cgcgcacccg ctgtgccgtt gccggcccga tggccgcgat
     6961 ccgcgcacct ccgatttcgc gcgcgtcgtc gtagagcttg taaaacatct cgaaaaatgc
     7021 gctcacccca ttcgcgctcg taaagacgat ccaatcgtag gaatgcgcat cctgcaccag
     7081 ttcggcgaag ccgcggaggt cgctcggcgg atcgatgcgg atggtcggca attcgatcac
     7141 ctcggcccca agattgcgca gacccttgct caacgcgccg gcctgtttcc gggtgcgcgt
     7201 caccacgata cgcttcccgg aaagcggccg ctcctcctgc caccgcaact ctttgcccaa
     7261 cgagacgact tcgccgaaaa cggccacccc tggaggcgca aagccttcct ccgccacccg
     7321 ctgggcgata ttttgcagcg taccgcggac cacttgctgc cggccggttg ttccccagcg
     7381 cacgagcgcg accggcatat ccgggcgaac accgtgactc atcatttgtt gcgcgatacc
     7441 ttcgatcctc tccaccccca tcagcatcac ctgggtaccg ccgagttgcg ccagcgccgc
     7501 gaaatcgatc gagctctccg atttctccgg gtcttcgtgg ccggtgaaaa acgtcacgtg
     7561 cgaagcgagc ccgcgatgcg ttaccgggat gcccgcgtag gccggcgccg cgatggcgga
     7621 ggtgactccc gggacgactt cgaaaggaat cgaggccgcc gcgagggcct gcgcctcttc
     7681 gccgccgcgg ccgaacaaga aaggatcgcc gcctttcagc ctaacgacct gcttcccggc
     7741 ccgcgctttt tcgaccagca gttggttgat ttcatcctgc tccagggtgt gcgcgccggc
     7801 ctgttttccg gcgtaaatta tttccaccgc ttccggcgcc cactgcagca tctccgggtt
     7861 gcagaggtaa tcgtaaacca ccacttccgc ccgctcgatc acttccctgg cgcgcaaggt
     7921 gaccagtccc agatcgcccg gtcccgcgcc gaccagataa caccgtccca gttttttctt
     7981 atccgccata aagttgcttc attagttggg ccgcgatcgc ttccggatct gcggccgaac
     8041 cggttgtcgt gccaacgcgc ggcgcggccg tttgcccctc aaaaatctgc gcccgcaata
     8101 ccagttcacc ttcgtttaac agcgcctgaa cacccaccgg cgaatcgcag tcgcccccga
     8161 gcaggcgcag gaattctcgc tcggcccgca ggcaagtgag cgtcgctcga tcgttcaccg
     8221 ggtcaatcca acgccgcgtc ttcgcatcgg cgcgccgcac ttgcagcgcg atcacgccct
     8281 gaccgcccgc cgggacaaaa tcgtccggtg acagaatttc gccctgcacc gcgagcccga
     8341 gccgctccag accgacccgc gccagaataa ctccgctcca gctctcgttc tcgcgcaact
     8401 tgcgcaaccg ggtcggtacg ttgccgcgta aatcggcggt ttgcaggtcg ggccgtttcc
     8461 agaccagttg atgctgacgc cggacgctgc ccgtggcaat cacgctgccc gccggaatgg
     8521 tcgccaggcc atctccggtt ttcgtgatca ggacatcttc cgctgcgccg cggggtggcg
     8581 tcgcccccag ttccaactcc tcgcgcggtt cgcttggcaa atctttcgcg ctatgcaccg
     8641 cgacgtcgat tcgcctaacg agaagggcct gctcgatctc acgcgtaaac attcctttgc
     8701 gcccggcttt gcggtccgcc aacaattcgg ttttcggctc ctcgtcgcct ctggtccgga
     8761 tcacctcgat cgctacttcc agttcgggcc aggcggcctg caatgcccgc tccaccagac
     8821 gcgtttgcgc cagcgcgaga tcgctgccgc gcgacccgag gattatcctg cccggcgcgc
     8881 ccactcagct ttcggaaggg cggagcgaag cttccggcaa ctcggccgcg cgcctcattc
     8941 gctcccccgc cgaaacgaac cagccgctaa aatcgtcgac gtgccccgca atgatctcct
     9001 ccccggcagc cacctgctcc cgtcgcagcg cgagtgactg ctgtgcgatc gattgcagcg
     9061 aatcaatgtc gtataaaaac acgccttcca tctcgttcac gtccggcgcg acgtcgcgcg
     9121 ggaccgcgat gtcgatgatg aacaacggcc ggtcgaggcg gtcgtgcagg agcggcgcaa
     9181 gattttctcg ggtcacgatt ggcgttcccg ccgccgtcga ggaaatcaaa atatccacct
     9241 cgcggcagtg cgtttcccac tcctccatcg cgaccgcccg gccgcagatc agcgccgcga
     9301 gcgactcggc ccggtccagc gagcgattcg tgacccggat gtcgcgcacg ccccgcttca
     9361 acaaagcccg cgcggttcgt tcgctcgctg cgccagcgcc cagcaacagc accttccggg
     9421 tctcgaagtt gccgaagatc tgttccgcca aatcgaccgc aaccgagcca accgaaaccg
     9481 cgccgcgagt gatatccgtc cgagtgcgca cttgcttcgc gacacgaaac gcggactgaa
     9541 aaagccggtg caagtaggga cctgcggcac cggattcacg cgcggcagcg taggcttttt
     9601 ttgcctggcc gaggatttcg gtctcgccga taaccatcga atcgagcccg gaagcgacgc
     9661 gaaacaggtg ctgcacacat tgctgcgctt cgtgtcggta gaaaaactcc gtttcgtcct
     9721 gcgccggagt catgccgggg ccgagcagac aacgcacgat gcgctcggtc gccaccggta
     9781 cctccgccgc ggcataaact tccacccgat tgcaggtcgt gagcaaaagc gcctcggtgc
     9841 agccggcttc ctcgcgcagc ttccgatcga ccgcagcgtg cccgacgaag cgctcgcgcg
     9901 tctcgacggt tgccgtgtga tgactgaggc cgacgcagaa aagttttttc acagtgtatg
     9961 ggttgacgaa aggaacgtaa taccccagag caacgtcagc gcggcgctga aggccacgac
    10021 gcagagcgcc gcgatccggc gcggggcggt cgtgcccaag tggcgcgcga gcaggatggc
    10081 ggcgtaaaaa atccacacgc ctaacgacca ggcgatcttg acgtaaggca atggctgacc
    10141 ggtgaagaat ccggccgcga tgcccaccgt cagcaaaatg aacccgagcc agagaagccg
    10201 ggtgatcgcg gcgaagagat cagtgagcgg cgggaaatga taaaacatcg aatggagctg
    10261 acgcgtcttg agctgacgct cttgcagcaa atacatcagc cccgcaacgc aggcgagcgc
    10321 gaacgcgccg taggcgatga gagaaaccga ggcatgcaat tccaggcacg gcgtgggcgc
    10381 gaccggcatt ggatgcgggc ggtcaatcgg cacgaggaga gcaaacgctt gcaagccgaa
    10441 gaccagcggc gcggtgaaag cgcccatcag cgaaagccgg taagccggcc cgatgagcag
    10501 gtaaatgact gaaacggacc aggccagaaa gacgaaaacc tcgaagagat tggtcaatgg
    10561 acagcgcccg agagcgtgtc cgcgcaccga aagaaaagcc gtctgaaaga cgaagccgag
    10621 cgcgatcgcg aagaagttga aaccgaccgc gtgataggtc cgcgctttga tcgcgaccgc
    10681 ggtccgcacc attgcgaaaa agaaacacag ggttgagacg atgagcagag tgcgatccat
    10741 cgccccggag tgtaaaaggc cgggcgacgc ttggcaatca gccttccgtt tcctgtctcg
    10801 gcgcgattcc tgctttccaa taggctgtcg atgaaaacgt tggcgcgcag atccagcggg
    10861 aacaagaaca cggcagacga agtcgagggc ctccgccgag tgcttgacca aagttacgcg
    10921 caattgcgcg aacgagcgcg tgcggaatgg caacgcaaac actccgatct cgacccgagc
    10981 gatgcctgtg tccgcgtctg tatccggggc aacggacagt cggtcctgac gtaatcgaca
    11041 cgcccgggct gggtctactt gccaccggtg ggaatgttca ccgcgaccgt ttcgaaattc
    11101 tccgaaacag cgacgcgacg catgccgttg aggaaacggc tgattttcca ccccctgaaa
    11161 ctgagcggag gctatgcccg ggaagatcgg ccgaaagacg ctgcgagtgc ccgttgacct
    11221 tttaactcga accggtagcc tgccgagatg gttgctgatt cgatcgctcg tttgcgtgcg
    11281 ggcattcgcg acgtgccaaa ttttccgaag catggcatcg tcttcaagga catcacgccg
    11341 atcttggtaa acgcccggct tttccaggaa tcgatcgatg tttttctcga acgctgccgc
    11401 accctgccaa tcgacaaagt ggtcgggatc gacgcgcgcg gttttctctt cggctccgcc
    11461 gtcgcctatg cgctcggggt cggcttcgtg ccgatccgca aaaaaggcaa gctcccgttc
    11521 aagacggcga gcgcggctta ttcgctcgaa tacggcgacg ccgagatgga aatgcatgtg
    11581 gatagcatcg agcccggcga gaagatcgtc ctcatcgatg acctgcttgc gaccggggga
    11641 acgtcggcgg cagccgcggc gctgatccaa aaagccggcg gggagttgct cgaggcgcag
    11701 ttcttgatcg agctggaatt tctacatggc cgcgtcaagc tcgacccgac accggtcgtg
    11761 acgtttctca agttttgacc gtggttaaac caaatcagcc gcaggcgcag ccggcgccgc
    11821 agcggccgct gcgatcttcc acgatcactt ccacctggat ctcctgcagc gcgagctgat
    11881 cgtggatcgc gttgagcagg tcgagcgcgc ggcctaacgg catctccgcg cagcgcagct
    11941 cgatccgcgc attcttgtcc gacagctcgg ggtgcgcttc ggccagttcg ccgtcgagca
    12001 agaacgaaac gtaggcgttg aacttttcct ggagctgaaa aagctgctca tcggagccat
    12061 cccacgggcg cggctcgaac atcacgagga gcacttcgtc ggtcttcgcc tcgtgcgcga
    12121 agaaatcgat gaccccggtc tgctcgaggc cgccaggtgg agcggcggcc gggtcggccg
    12181 caggcccgga agacatctca ggctccgttt cagatttctc tcccatcggg agcgcgacct
    12241 tacggaaagc ttggtgattt cgcaacgacc cgctaggata aattgtgcca tgcgaaaatc
    12301 acctctcgcg ctcgtcgcac tcgcgatagc cgggacgctg cccgcttccg ggcagttcac
    12361 tcccgggttc gtcaccaaca gcggctactg gaacgacggc aaggccgagt tcaacatcta
    12421 cgacgcgcgg atcgttcgag acggccagcc gcgccagacc gaggtgctcc acatcctggt
    12481 gcgcgagccc ttcgacctga ggcagatggt caaaccagac aactggcaac gccccaacgt
    12541 catccaggtg ctgaagatga atcagattct gcacatcccg acgggcctct acgtctttca
    12601 acagatgcat tccaactttt ggcgcgccga taacgcgcgg ctggccaagt tttccctcac
    12661 cagcaacgac agttgcggca acacctacaa ggaagcgcgg cgtggcggcg aggttttcac
    12721 ctgccaatgg cacacttact gggatggaat ggccgagggc cgggaaaaag tgcggcttcc
    12781 gcctaacgga ttcttttacg atgagttgcc gcttcgagtg cggacgatcg atttctcgaa
    12841 aggaagcggc gactttgcga tccagctcgc cccgaccatc atcaactcga agaaggacaa
    12901 cctcgtctgg aaaccggcca aggtgcactt cgaatccacc aaccgggcga tctacgtcag
    12961 cgtcgagcac gcggcgggga ccgaccgctt catgctcggc cgcgggttcc cgcatctcct
    13021 gcgtgagtgg gcggcggccg atggcagccg cctgacctta aaacgaagtc tgaagatcga
    13081 ttactggaac taccacaacc tcggcgaccg caagcgcgcg ctcctcaatt cgctcccggc
    13141 acgtccggac taacgaaggg cgaactttcg cttcaacttc ggattgaagc gcggttaacg
    13201 aactgtagct ttcccggtgg cgaagaagac ggttaacaaa cgactcaccc tcctcggtcg
    13261 gagcgagacc cggatcccgg ctgctccttc cgccgcaacc ctcgagacct ttgcgaatcc
    13321 cgcgcgccgg ccctattgga tccggtttca ctccgcggat ttcacctcgc tctgtcccat
    13381 caccggtcag gcggatttcg cgacgatcac gatcgactac ctgcccggcg cccgctgcct
    13441 cgaaaccaaa tcgcttaagt tctatcttgc ctcctatcgc aacgagcggg ctttcaacga
    13501 aagcgtcgcg aaccgcattc tggacgatct cgttagggcg tgcgcgccgc ggaagatgac
    13561 catcgaagcc gaattcgccg cccgcggcgg catcacgctc accatcaccg ccagtcaccc
    13621 ggacgtgtcc gcgcgatgaa gcgcgccgtc gtgctgctta gcggcggcct cgactcggcc
    13681 accgtgctcg cgctttgccg cgccgaaaac tacgcggcct acgcgctttc ttttcgctat
    13741 ggccagcgcc acgagcgcga aatcgcggcc gccaggcgca tcgcaaaatc gttaggcaca
    13801 acggagcatc gcatcgccga gatcgatctg cggctgttcg gcggatccgc cctgacggct
    13861 gacctggccg tgccgaaagc gcgctccgaa gtagaaatgg ccgcgggcgt cccaatcacc
    13921 tacgtgccgg cccggaacac catcttcctc tcctacgcgc tggcctgggc cgaagtgctc
    13981 ggcgcgacgg atatttatct cggcgtgaac gcagtcgatt acagcgggta tcccgattgc
    14041 cggccggagt tcatccgcgc gtttgaacga ctggcgaatc ttgccacccg cgccggcgtc
    14101 gagggcgcgc gcttcgagat tcacgccccg ctcctcgctc tttccaaggc gcagatcatt
    14161 caacgcgggt cggaactcgg ggtcgacttt tctctcacgc atagctgtta cgacccgact
    14221 ccggacggtc tcgcctgcgg ggaatgcgat tcctgccagc tccggcgccg aggctttcgc
    14281 gaagccggcc gggaagattc gattcgttac gcacgatgaa cctgcgcgcc aggctcggca
    14341 tgttcgtcct cacccgccag gaacagcgcg cgattgcatt catcgtgctc atgctggtgc
    14401 tcggcctggc gaccaggcat tatcgtcaca ggcatccgaa atccgcgaac tcgcctaacg
    14461 aaacagtccg ggcggtgccg acgccgacgc tggcgccggc caaaaacccg ccgtaaccct
    14521 ctctgtcccc tcgcgccgag tcgaagccgg cgctacagcc cggggatgcg gccgtagccg
    14581 acgaatcgcg gctggccctg agaggaaggc ccatctttcg agggcgccgt tttgaaatcg
    14641 aacacgctca caccaaaacg ctctttcccg tgaaaggtcc gaccatcgct cgctccgatc
    14701 atgattggtt ttcccgattc cttttccctg ccgaggtaaa tcatggtgtg cgtgactggc
    14761 ggatcgcgct ccacgctgta ggtgccggtc cagaaaagca gatcgcccgg cttgagttcg
    14821 tcgagttcga aggaatcgag atcacggctc agaacggcgc ggaaattccc cgctttgcgg
    14881 acccagacat attgttcgct ggcatcccgc gggacgtcgg gcacaccgtt ttcgcgcaag
    14941 aggtagtaga tgaatccgga gcaatccatc ccgccgcccg cgggatcggc ggagccgtag
    15001 aggtagccga ggttgcgctt cgttaggtcg agcgcgtccc cgagtagttt ctgcacggct
    15061 gcaggattct tgtcgtaacc ttcaatctcg tccggcgaga ccgcggcgac ccgcttctcc
    15121 gggctgggtg attcttcggg ttttggcgtg ggtgtttttc tcggactggg cgaggcgctc
    15181 ggggacggac tcggcgaggg ttttttgcgt ttcggtttgg gcgtttcctc ttcgtcggga
    15241 gtgggcgtgg gtgtttttct cggactgggt gaagcgctcg gggacggact cggcgagggg
    15301 ttttttcgtt ttggtttggg cgtttcctct tcttcgggag tgggcgtggg aaaactcttg
    15361 tgtaggcgct tcgcggtcga ttttggagca gcgctggggg acggcgacgg tgtcggactg
    15421 ctcttcttgg tcacgcgctt gtgcggccga tggctcggcg tcggggtcgg cgtcgcgagg
    15481 aatttcctaa tcttcgcgcc taacgaaggt tcttccgcgc ccaccccttg cgtcggcccg
    15541 agagagataa gaagaaggag ggtgatcgct ccgacgtcca gccgattcgt taggcgaaga
    15601 atcacgcgcc gattgttcct tgtcatgccg ggcgaagtag agggatttcg catccagaga
    15661 gatcggtccc gatcctgccg gtggacgcgt ttccggggag cgcgcgctcc ctcgcctacc
    15721 gcccgcccgt cccgatctcc tgcaggatcg ccttgttcat cttgatcccc gcctccaaat
    15781 tctcgagcgg gaagttttcg ttaggcgagt gcgcccggca gttgggcagc gcgagcccga
    15841 gaagcagcgt ctcgaccccg agaatgtcgc gaaactgcga aacgatcggg atgctgccgc
    15901 cttcgcggat gagcgcgact tcagccttga aggcctcctt tagggcgtat tgcgcggctt
    15961 ggccaaactg cgaatgcggg tcggtcagat accaggggcc gctgtgcccg tcggtgatct
    16021 ccatcgtcac tccgggcggc agatgttttt ccaaatgctg gtgcgccaat ttcaaaatcg
    16081 catcgccatt ttggttgggc acgaggcgaa aagtcagctt gaccatcgcg tgactcgcga
    16141 tcaccgtttt cgttcccttc ccctgatagc cgccgccgat gccgttgatc tccgccgtcg
    16201 gccgcgccca aagccgctcc agggtactga agccgttctc gccgaagagc gccggcgcgc
    16261 cggtctcagc gagaatttcc ttgtcggcat tgatcggcag cttgcgccag gcttcccgct
    16321 cccattcttc gagcggcttc acgtcgtcat aaaagccatt gatcgcgaca tggccgctat
    16381 cgtcgtgcag ggtggcgagc agtcgcgcca gggcggtgac cgaattggcc accgcgccgc
    16441 cgaaaactcc cgaatgcaaa tccatcttcg cgcccgtcac tttcacttcg cacgccgtca
    16501 cgccgcgcag tccgtagctc agcgtcggca cgccgcgcgc gatcatgccc gtgtcggaaa
    16561 ccaccgccac gtcacacttc aacgcggcgc gatgctcctg caagaaaccg ggaagattgt
    16621 tgctcccgat ctcttcttcg ccctcgatga tcaggtgcag gttgaccggc aggtcgccgt
    16681 gttcggccat cacttcctcg atgccaagaa tgtgggctag aatctggcct ttgttgtccg
    16741 tcgcgccccg cgcatagacg tagccgtctt tcagcacggg atcgaacggc ggcgactccc
    16801 aaagctcgag cggatcgggc ggttgcacgt catagtgtcc gtaaagcatg acggttttgc
    16861 ggcccggttg atgtttgttc ttcgcccaga ccaccgggtg acccggggtg gaaacgagct
    16921 gcgcctcgag gccgataccg tttagctttt cgacgagcca acgggcgcac tcgccgagct
    16981 tctcggcata tgcgtcgtcg gtcgagacac tgggaaaccg caggaaggca taaaaatctt
    17041 ggagatgaga atcgcgcatc acgaagagta gaacagcttg gcgcgacgac aactaacgac
    17101 ttgccggagc gacgcggctc gcgcagcctg cggcccatga gtgcgcccgt cgagcctgcg
    17161 gtggagaagc aattgatccg tgcgctcggt gtcccgaccc tggcggcgaa catcgtgaac
    17221 tcgacggttg gggcggggat tttcgcgctg ccggcactga tcgcggcaca actcggcacg
    17281 gccgcgccgc tcgcctttct cgcctgtgcg ctggcgatgg tcgtcttcgt cacttcgttc
    17341 gccctcgccg gcagccgcgt ctcgctaacc ggcggcatgt acgcctacgt ggaggtcgcc
    17401 tttggccgct tcggcggatt tatcgccggg gtgctctatt tcctcaccgc gatccttgcg
    17461 acctccggca tcgtgagtct gctggccgac acggtcggta ccatttctcc gctcttcgcc
    17521 aacaagcccg cgcaatttct ggtcgtcctc tcggtcctcg gatcgctcgc ctggattaat
    17581 gttcgcggcg tgcaatcggg cgcccgcgcg gtctggatag tcacgatcgt gaagctgatc
    17641 ccgctcttca tctttgtcgg tgcgggaata tttttcgtta ggcaggccga tgtcctgacc
    17701 atgtcctggc cgggtgcttc cgccctgggc cgcagtgtgc tgattctgct cttcgctttc
    17761 gtcgggattg aagttgcgct cattccgagc ggcgaggtga agaatccgac ccgcaccgtg
    17821 ccgcgtgcga tctaccttgc tctcgccgtc acgaccgcgc tttacctcct catccaattg
    17881 gtcgcgcagg gaatcctggg aggcagcctc gggaagtttt ccgccgctcc cctcgccgaa
    17941 gcggccgcgc gttttctcgg cggcccggga cgcagcctgc tgctcaccgg cgcaagtatt
    18001 tcggcttttg gtttcatcac cagcgatatc ctcagttcgc cccgaattct cttcgccttt
    18061 ggacgcgatc gctttcttcc gcaggctttc gcgcatgtcc atcggcgttt ccatacgccc
    18121 gacgtcgcga tcatcgtcta ttgtacaatc tgcgcgatcc tctccctcag ctcctctttt
    18181 cagcagcttg ccattctttc gaacgtcgcc gtgttggcgc tctatttcct cagctgcgct
    18241 gcggccgtcc agttgatccg ccgcgatgtc cgagccgatg gaccaccgtt cgcctttcgg
    18301 ggcgcgtgga tcatgccatg cgtcggagtc gtcattagcc ttttgatcct cgcccaggcg
    18361 aaaagagagg aactgacact gaccgccgtg gtggtggtgg tggcggcgat tgtttttgcg
    18421 gtccaacgca cagtcaggaa gccagcctgt tagccacccc gataccatat cttgtgcccg
    18481 gactgtcgcc cgccccaaga tagcgtttat tttccttgtc accgccggga gcgggtagta
    18541 tgcacctcct agttttccta ccgcatgcac cttcaatcgc tcgagctttt tgggtttaaa
    18601 tcctttgccg ataagaccgt tttcaatttc cacgagggcg tgaccgccgt cgtcgggcct
    18661 aacggatgcg gcaaatcgaa cgtcctcgac gccgtccgct gggtcctcgg cgagcaatca
    18721 gccaaggcgt tgcgtggcgg cgaaatggcc gacgtgattt tcagtggcac cgattcgcgc
    18781 aagcccttcg gctacgcgga agtgtccctc acgtttaccg actgcgctac cgagctcggc
    18841 gtcgattggc acgacgtgcg cgtcacgcgc cgcgtttatc gcgacggaat atcagaatac
    18901 ctgctcaaca aaaccgtctg ccggctgaaa gatatccaga gcctttttgc cgacaccggc
    18961 gtggggcgga gcgcctattc gatcatggag cagggccgga tcgatcaaat cctcagctcg
    19021 cgtccggaag atcggcgcgc ggtctttgaa gaagccgccg ggatcacgaa atataaagcg
    19081 caaaagcgcg aggcgctgcg caagctcgag gcgacggaag cgaatctgct ccggctcggc
    19141 gacatcatca aagaggttaa acgccagatc ggctcgctcc agcggcaggc gggaaaagca
    19201 cggcgctacc aggcggtgct gggcgatctc caggtgctcg atacgcatca ctcgcgccgg
    19261 caacttgatt cgctcgaagc cgaacttgaa cgttcgcgcg aagcgatcgg acgcttcgag
    19321 gaacagcaac acacgaccga gcgcgcgatc gaggaacacg aaaacgaagt cgaggcgcaa
    19381 cgcggccggc tggaggaagt cgacgcgcgg atcaacgacg cgcgggcggc ggtgcagggt
    19441 ctgcagaacc agcttacggc gcatcgcaac cggatcgatt tcaatcgcga acgtgcgcaa
    19501 gaattgagcg cgttaatcga gcgctacgag ctcgatgtgc aaaccgcgga aaccaagcgg
    19561 gcgcaacagg agacggaaat ccacgatacc gatgtgctgc tcgcggaaac cgaacgcctg
    19621 ctcgcggcga aacaaaccga actggagcag ttgagcgcgc gcgcgggtga ggtgcgacgg
    19681 cagcgggaag ggcgcgagcg cgaactgcaa acgctcgagc tggccatctt gaaggcggaa
    19741 aaccgactgg ccgatctgga aacggaaatc tccggaacga tcgaccgtcg caccgccacc
    19801 gagccacgcg tcgcggagct tgcggcatcg ttaggcgaac ttggtggggc gcgcgatctg
    19861 gtcgcaacgc aactcgccgc cacccgggcc cgcgctgatg agcaacagga ggcgttccag
    19921 gatttggccg cgcaagttcc cgccgcagaa gaagagcttg gccgccagca acaacggctg
    19981 cgggaggcgg aaacggcgct catggcgagc gaccgcgcgc tcgcggaacg gcagtcacgt
    20041 cttgagatcc tgcagcagct caacgccgag ggcgaaggcc tgacggaagg ctcccaggcg
    20101 gtcctgcgcg ggctcgacga tccagcccgc attctgccgg cgctgaacgg cgccctcgcc
    20161 tcgcttatcg aggtcgaaga gaaatttatt cccgcggtcg aagcggcgct cgggcgtaac
    20221 ctgcacgcca tcgtcctgca aaatagcgcg ctcgcgactg aaatcatcgc cgccgtcacg
    20281 gcccggaagc tcgggcaaac cgcgctcatc gcccccgagt tgagcgtggg tacgccggtc
    20341 gacgcccgca tcctgccccc gggcgcgctc gcctgggcga ccgaaaaaat tcacgcttca
    20401 gaccatctca taccactcgt taggcactta ttaggcgagg tcgcgctcgt ggccgatctc
    20461 gagcaggcgc aaaagttgaa gaagaaattc ccggccctcg ccttcgccac cctcgccggc
    20521 gaactcgttt ccacccaagg cgtcatcttc ggcggaagcg tcgacggaga aaacagttcg
    20581 ctctttgcgc gcaaggcgca gattgccgcc ctcgccgcgg agcatcgcgc gctcctcgaa
    20641 gaacaacaaa atctccgtgc gcagcacgac gaaattcagc gtgaactcga cgccgcggcc
    20701 gagcgcctgg acgaaatccg cgagcagcat caagccgcgc gcggggatca ttccgcgacc
    20761 gcgctgcaag ttagcgcgct cgagcgcgag tggcacgaag ccggtcgcaa ggtggacgcg
    20821 ctcggttggg aaaagacgac gcttcatcag caactcacca gcgccagcga gcgggtcgcg
    20881 caattggaaa gcgaagcgac cggccagagg gaattcctcg tggccggccg cgaacgccag
    20941 gtcgcgctgc aaaccgccgc cgaggcggcg cggttgcgcg acgagcaact ggtcgagcaa
    21001 ttgagtgaat tgcggctcgc gggcgcgacc gagcgccagc ggcacgaagg actgcgcagc
    21061 cagcgccagc cgatggccgt ccgccaggcc gaactcgccg agttgatcgc ggcgcggcgc
    21121 gcggacatcg caaattatca ggcccggcgc gagcagcaga gcaccgagag cgccgccgcc
    21181 gagcgcggaa tcgaggaaaa cagcgcgcaa ctttcaggcg ccgaatccgc ggtagcggaa
    21241 atcgccgaaa cgcgggcggc gcgcctcctg gaagtgaata cgctggagac cgatttgcgg
    21301 gcgcgccgga aagcgctcag cgatctgcac gacgcgcgcg gcaaggaaga ggtccgccag
    21361 acgcaaatgc agctcaagat cgaaagcatc gccgagcatg tcatgcgacg ttaccaagtc
    21421 gacctgcgcg agtttgcgcc cgacagctac gcattcacga aaactttccg tgcgcagctc
    21481 aaacagcgtg ggaaatccga gccggacgac acggaagcct tcgatgaagc gccgctccgc
    21541 gaaatcatcg accaacttac ccgccagctc gacgcgatgg ggccggtgaa cctcgatgcg
    21601 gtgcacgaat acgatgcgct ggaggaacgc catgccttcc tcgaaacgca aaacaacgat
    21661 ctcacggcgg cgcgccgcga actgctcgat gtcatcacgc gcatcaacca gacgaccgag
    21721 aaactcttcg ccgaaacttt cgcccaggtg cggatcaact tccgcgaaat gtttgccgag
    21781 ctcttcggcg gcggtcgcgc ggacctgacg ctgcttgacg agaacgacgc gctcaactgc
    21841 ggcatcgaca tcatcgccaa gccaccgggc aaacaattgc aaagcgtctc gctcctctcc
    21901 ggcggcgagc gcacgatgac cgcggtcgcg ctcctctttg ccatctacat ggtgcgtcca
    21961 agtcccttct gcattctcga cgaaatggac gcgccgcttg acgagtcgaa catcaatcgc
    22021 ttcatccgcg tgctcgagcg cttcgtcgcc caaagccagt tcatcatcat cacccacaac
    22081 aagcgcacga tcgcgaaggc cgacattctt tacggcgtga cgatggaaga acgtgggatc
    22141 agtaaactgg taggcatgcg gttgacgggg ggaaacccgg aggaagaaac gcgaccaatg
    22201 cgcgccgaac cacggaccga acaaagggcc ctcgaactcg acgacgcgcc agcggaaaat
    22261 gtcgctctca ctcgttaggc aggtagctct gcggcacacg ggatatgcag aatgccggtc
    22321 cgccggaggg acgcgctgcc gcgcgtccgg ttcgcaagag ttcgcattaa attaaacggt
    22381 cgagcaaatg agaaagcgtt tccttggcct gtttctctta accgccgcca ttgtctttga
    22441 ccccgggtca ccgaccccgg tgcaagcggc gccctaacga gcagtcgaaa gcgccgctcc
    22501 cgcgtcgccc gccggcagat ttgccgcctt ccaatccatc gccatccgga tgcgggcgcg
    22561 gccgctcggg tgatcgaaga agatgaattc ctcgaccgga cccggatcga gcttgcgata
    22621 gacgcccagc ttgagcgccg ccttcgccat gccatcggct tcgcgcgcgg tgttgatgcc
    22681 gaaggcatcg gcctcgcgct cggtcactcg ccccgccgtg ttggtgagcg gggtggcaag
    22741 aaaaaagaag gtcgaaaaaa tcaagacgag cagtggaaag cccgccgggt ccgcgatgtc
    22801 ccgtacgccc cagcgcccgc cccaccgacg caccgcccgc tcgaaaattg cgcgcgcaac
    22861 ggcgaaacca ccaaaaataa aaagagcaaa ataaaggatg agcttgaacg agtgattgag
    22921 cacgtaatgg cccatttcat gcgccatcac ggcgcggatc tccggcaggg tgcattgctt
    22981 gagcaggttg tcgttcagcg aaatgcgcgc ggtcccgaag agtccggaga cattcgcact
    23041 cacgcgattg ctctgacggg acgcatcgac ctcgaacacc tgtcgcacgg ggatttcgtt
    23101 agcgctggcc atcgccagga tcggctggct gatcttcggg tcggagatcg gtttgtaggt
    23161 attaaagagc ggggcgacga aaaccggcgc gatcagcgtg gcgatggcaa gaaaaagaac
    23221 ccccgccccg gtgccccata accaccacgt ccgccgggcg caccggaaaa cggcgtagag
    23281 aacgagcaaa gcgatcgtgg ttgcaatgat cgcgatcgcc agatcgatga gctgttcgcg
    23341 aaaccagggc ccgaaggttt gcgtcgccag cccgtaagca tgttcccgga aatactcgga
    23401 gtaaacgcct aacggaaagg aaaaaacgaa aacgagcagg acgtagggaa cggcataaag
    23461 cgcgatctgg agcgaccgga agcgggtagc acgctccgcg aacgcacgca agcgcgcgga
    23521 aagaccactg cttaatagga aaacggagat cgccgctccg agcaggaaat tccagaggat
    23581 cagccagtag cctccctcga agtaggcatc cgaccgggct cgtttctcgg ccgggacggt
    23641 ggccagccag gcgcgggtag ccgcggccgg atcgaggggt ttctgcgttt ccggcgcggt
    23701 cgttagggag atggaggtgg gttgcgcgat aacgatcagc ggggcggagc tcaaaaaaca
    23761 agcggtcagc agcaagaaaa cgattttcat gaagccggag gaataaacga attcgccccg
    23821 aaacagaagc ttgttccccc acggtctcga cttccgcagg ctcgcagtta tcttgttagg
    23881 tgaatcgggt gggcagagtt tttctggctt gggtggcgct ggcggcgttt tggcccgtga
    23941 ccgacggcgt cgcggctaat ccgccgccgg ccaatgcggt ttacggtgcc tacccgacca
    24001 actatcagga cattgtgacg aattggttaa acgccgccct ggtcgacccg aagagtgtca
    24061 aagtgaaatg gctgggcgaa ccgagaccgg gggaattgcc ggtcggcaag ggccagaaag
    24121 taaccggatt cctggtcgat ttctcggtca atgcgcgaaa cattttcggc gcctacaccg
    24181 gttcgcaaaa gcataccgcg ctgattcgcg acggccaggt ggtcaccgcc accggttttg
    24241 tctatcacta agaaatggag cctaacgaaa aacgcggata cgtcatcgtc gagatagaag
    24301 tgaccgatcc tgcgcgctac gaagaatatc ggcgccaggc cgaggccacc gtggtttccc
    24361 aaggcggaaa atatgtcgtc cgcggcggcg gcctgcagac attggagggc gactggcagc
    24421 cgaaacgact ggtcgtgctc gagtttccga gtgtcgcgca agcgcgcgcc tggtggagtt
    24481 gcgaagaata ccgcgcgccc aagaagatcc ggcaacagtc ggccgtcact cggatgattc
    24541 tggtggaagg cgtctgagct acggcttggg cgccgccaac tggagaacgg ttgggggccc
    24601 gggcgacacc attcggagga gcaaggggcg cgcatccggc gcccggggat ggacgtagaa
    24661 agtcgcttgg gagaccgcat catccgaggc cggtccttcc gatgttatcg tcaggctgag
    24721 ggccccggag ccgatccctc ccctaacgaa agcctcccgt aaagacggca aatcttcgct
    24781 cgttcccttt cccaggagtt gcacggtcga gagattggcg ccgccgccgg gcaggcccac
    24841 caactgcagc gcgtcgtcga ccggggtcag cgtcagtcca aaggccactg gagtagttgt
    24901 gaggcgcccc cgcagcccaa ttggagtcga gttgatttcg aaccgcaaag gtgcaatcac
    24961 cgcctcagtg tttttgaact ggagttgagc ctcgaggccg gtcagttccg gccatggcgc
    25021 cggcggtttg aagcgcgcct ccaggatctg agcagcgccg ttgatcgagg gaagctcgag
    25081 cgtattgaaa aacgcgatgc caccggtcac tagcccgctc tcggcgccaa accgcaattc
    25141 gttaggcaag gtgtcgagaa aaagcgcggg gcaatcgatg gcaaccgaga attgacctgc
    25201 cggctcgatc accgtcccgg cggcgaggcg gtcttttccc agctgcaagg gcagccaggc
    25261 gcggagcatc accgggtcgg acacgccaaa aatcgctgtt gttttcactt cggcccgacc
    25321 atcggcataa tgcccctcaa gatcgattga gttgctcgac gtggccgggc cgagattctc
    25381 gagccgccag ctggtcgtta cctggagaga gcgcagcgga ccgaaggcgg cgagtttgcc
    25441 gtcgacgacg ccggtcgcgg ccgaatcctc cccaagcaca ctcgcgagct tggccacgtc
    25501 gaggtgatta atcgccagat cgacgtcgaa tttctggcct tcatccaggg ctgcgagcag
    25561 gctgcccgag gtgcgccagc gctcgaaact gaggggcaga aagagcgtcc cgctgacccg
    25621 cggcatcccg tcgagggtca attccagcgc ctgcaactca acgaaattgc tcccaagcat
    25681 gaggaaactg cctaacgaca cacgggcact ggccagcttg aaggtgcgaa aaaaaacatc
    25741 ctgcggcgag taggtgccga ccagggtcac atcgagcggc agacgcaacg gcgcgaccgg
    25801 gagctgcagc ccgtgggcaa agaattgcat cgttccagaa tgcgcggcca gcgttccgtc
    25861 gccgctccag tcgaaggtca cgcctccgcc gattttcgac gaccgccacg ctgccggcag
    25921 aaggggcgcg taggcgccca aatcgttgat cgcaccagtc agacgcccgg aaaaacgatg
    25981 gggggaagcg agatcgatcg cgccttgcgc gcgggtgaaa tcttccgcat gtcgcacctc
    26041 cagattttcc agggtcgcct ccgtgcccct cagctggagc gcggcgccta acgaatcgag
    26101 cgcgacccca cgaaacgtca ctccctcccc gtgcagtgcc agccgcccat gcgcctccgg
    26161 agcgagcgaa tcgagttccc cttccgctga aagcgccccg gtaaaatcgc ccgtcgtcgc
    26221 cccgagaaac tgcgcaaagc cgttgaggtc ggggatgaag gcgttcaact gcccgcggaa
    26281 cggcaaccgc gcccaatcgc tcgcccgctt cggccagggc agttcgccat tcaccgtcaa
    26341 ttcgttccgc cgctggcgca cgtagagttg atcgacttcc aggcgccgat cgtagaaggt
    26401 cgcgccgagc atgacattgt cggcccgtcg cgcccgccag gcgaagtcgg tcagctcgat
    26461 ccagagcgac gcggtcgcat cgagaaactc gcctggattt ccgcgaaagg tgaacttcga
    26521 ggcgcgcacc gcgccggtga tcggctccag aaaacccatt cccttggaaa tctgcgccag
    26581 cgaaagattg gcagccgagc ccgcgacatc gacctcaaac gtggcgttcg cccgaccctg
    26641 aaaactggcg cgcagggtcc cgccataggt atcgagatgg agatcgagtc cgaggcgtct
    26701 cctgcccaga tgcgacaggt cgatggtcaa cccctcaaga tcgagtccct ccaccagcgg
    26761 gatgccagcg atcgttaggc ggtccccttc ccaggaagtc gcgccgcgga gattgccgaa
    26821 tgtctggcgc aaaaccggcg aactcaccgt gacctggcgc gccagaaatt ttccggattc
    26881 aatcgcgctc gccgtcagga agacatcgcg caaactcagg gccgtctccc cgacggcaag
    26941 atcgaaatcc aggtgctcga tccgaaagtt gttcggaagc agccgcgcca gcgtccgcca
    27001 atccagtttc ctagccgaaa ccgccccggc cggcagacga atgcctgccg tgggacgatc
    27061 cagattgatc gatcgaagca ggcgcgcccg gcgggaaaag atccaaccgc ggaaattgag
    27121 atcgacgacc acacgggcgg cccggaaaga aacctcgcgc gaggcatcgc cggcgcgcgc
    27181 gacgcgcaga ttgcgaatgg tgacctcgcg caggaaaggt gcgtccacct cgcccagctc
    27241 gacggagagc ttctgggtcc tggcgaccca ccaaaaccaa agcctgacgc catgcgcgac
    27301 gacgacgggc gcgaagagag cgtcgagcgc gccgcagagc agcacgacga gaaggaagac
    27361 gaacaaacgc ttgcgaaacg acatcagcct ggcagcctaa cgacgaacgc ccctacccgc
    27421 caggggacgg cgccggaaag attgcacgca ctggttgcgg agggtgaaat ttttctcgca
    27481 gctcccgggt gaaaacctgc tcggcttccg cgggcagcgc cgccgacttt tcgcgggcca
    27541 gttggggcag aagagcgagc tcggtctgga tgtcatccgg cacgatccgg ttccagagcc
    27601 cgttctcgag agcgaggacc tcgatctgct gctcctcaac ggagagaatc tgggcatggg
    27661 gcaattcgat caagatttcc tcagggcgga ggttgacgct aaattttttc ctcaaatcga
    27721 atcctgcttt gaccacgaac gtgccgtgca acttgatccg tttggtgctg ccggcccatg
    27781 aatgtaacat ttcgtgctca acttccaccc ggcgggcgag gaccgacagt tcggcgaccg
    27841 tggtcgtctg ttcgaggtag acgcgattgt tcacgatcac gcgtggctgc aggtgcgcga
    27901 gcgtgacgaa ggtgtcgcgc gcgtcgcgcc cgagacgctc gagctcgacc acgctctgac
    27961 ccaccgctcg tgcgggccat ttctgcaccc tggaaaagag ccaggcggcg agcagcgcaa
    28021 ggacgaaaac gatgaggacg gccgcgccgg ggcaacccag ccgccgcggt aacttttcgg
    28081 ccgcggggcg gctcgtttgt aactccatca gcccaaaaaa gtaccgcccc cacgcccgaa
    28141 ggacatgagg gcggctgttc cccccagaac aatctttcaa atctgaagcg gagatagccg
    28201 acctaactcc ggcatgcaag aaaatttttc tccccgggtg ggaaaaagtt ccagcctgtc
    28261 ctaatcttgt tccgtgaaaa tcggcgtgcg ctacgaaact gcctaccgct ataccgcccc
    28321 ggtcagtttt atgacccacg aaatccggct tttcccccgc atcgacgtct tcacccggat
    28381 gcaccgaatg aagtttgaga cgaacgctgg tgcggatgtg cgttttgggc gcgacgtttt
    28441 cgacaattgc gtcgccgcct gttcctttgc cgcgccagcg acggagctgc gttttcaact
    28501 tgagctcgat ttggagttgt tggaaaaaaa tccgttcgat ttcctgctca cgaccgacgc
    28561 cctcactctg cctttctcct acccatccga gttgcatagg gtactcgctc ccttccggga
    28621 aagcgacgcc ggtcggttgc tcgaaattcc gcgctgggag cggccgcaga ctggcgcacc
    28681 ccggccgacc gtcgcgaccc tcgtcgagct gaatgaaagg ctgcacgaag gtgtcggcta
    28741 cgagcggcgc gaagaaggac cggcgcgtct tccggcagag accctgcgca tcgggcgcgg
    28801 cgcctgccgc gatgtcgcgc ttctgctcgc cgcgatcctt cgtcaatgcg ggctggccgc
    28861 tcgcctaacg agcggatatt tacgtgaagc ggaatcggat ttgcgccgcg cggaagggtc
    28921 cctgcatgcc tggaccgaag tttacctgcc tggggcgggc tgggtgggga tggatccgac
    28981 caacggtgtt ttcgccaacc ataatttcat cccggccgcg gttggaatca cccccggcga
    29041 cgttacgccg atcgacggca gctttttccc gcgacaggca ggcagtgcgc aaatgacgag
    29101 ccggctggaa ctgctcagcc tctaagcgcg cgcgtcatgc ttcgcgatct gcaactgcgc
    29161 gatttccgct gcttcgcggc gatcgccgtt gaattctctc caggcttcaa tttcattgtg
    29221 ggcgcgaacg gccaggggaa aacgacattg ctcgaggccg cctgcgtcct cctgcgtttg
    29281 caatcgcaac gctcctcgag cctcgcgccc acggtgcggg ccggagggcg ctcgtttatt
    29341 gtctccggcc acgccgggga acacctattg caattttatt acagcgcgct gcggcggaaa
    29401 gtggccttcg atggcgtcga acagcacgat caggcggagt atttgcgcct tttcagggtg
    29461 gtttccttcg ccagtcacga cctcgaattg atccgcggcc cgggcgaaat gcgacgacgc
    29521 tatctggact ttatcggaac gcagacggaa gcacgttacc ggccggtttt gcgcgcctac
    29581 gagcgggcgc tccgatcgcg gaatgtgctc ttgaaatcgc cgcacgcgcg accgcgagaa
    29641 ctggcggcct ataccgagcc cctggtgcgg gcaggaacct cgttgcgcga gatgcgggcg
    29701 acactcgtta ggcggttggc cccttgcgcg gaaatcagcc accgcgcgat cagtggcggc
    29761 cacgaaaacc tctcgctcga atacgcgccg ggcagcgaag aggatttcgc cgcgcaactc
    29821 gcgcgaacgc ggacggagga agggcgcctc cggctcacga tcgtcgggcc acatcgcgac
    29881 gatttgcgcc tgcaggtcga agccatgcct gccgcgcact ttgcttccga gggtcaacag
    29941 cgcaccatcg cgctcgcctt gaagctggcc caggcgcagg ttttcgccga ggacgcagcc
    30001 accccgccgc tcctcctggt cgacgacatc tttggcgagc tggatgccgc gcggcgcaac
    30061 gctctgctcg cccatttgcc taacgagtcg caaaaattag tgacggcgac cgcgctcgat
    30121 tggcgcgcgg gcgattgggc gggaccggtt ttggaactgg ccgacggcca gattcgccgg
    30181 cgcgacaagt aatcaggacc gctgccccgc gacggtttca tcgaaccccc tctcagctgg
    30241 ggcttgcgaa cgggcttccc acgccttcta ttttcctatg cgccgccagg cggggaagcc
    30301 gcagaacttc acggggcagt tgctcgtcgc tcatcccagc atgctcgacc cgaacttccg
    30361 gcgctgcgtg ctcttcatct ccgcgcacga cgaaaacgaa ggcgcggtcg gtgtaatcat
    30421 caaccgtcct ttggataaac atgtctccga gctggtgacc gatccgacgc ccgccgcgct
    30481 cgccgacgtg cccgtgttcc tcggcggccc ggtggcgacg aaccagctga tgttcgccgc
    30541 gttcgactgg aagggacgcg attcgcttag cttggatcag aacgtcgccc tcgaggaagc
    30601 gcatcaactt gcggagcggg atcgcagtgc ggtgcgggcc tttgtcggct atgcgggctg
    30661 gactgcgggc cagctggaga acgaattgcg ccaaaatgcg tgggtgctgc aagcgccgaa
    30721 tcgtgcggcc ctcacaccgg agcgattacc caagctatgg ttcgaaatca tgcggggtct
    30781 cggaccctgg ttcaagatgc tgtcggcggc gccggatgat ccttctttga actagcttca
    30841 cgcggttttc gatgcgaagc agtgcggata tatgatcatc ggcatcccga aagagattaa
    30901 gcagcaggaa aaccgcgtca gcctgttgcc ttccgcggct tatcagctga ccaagcgcgg
    30961 ccacactgtg ttggtggaga aaagcgccgg cgtgggcacg ggttatcccg actccgatta
    31021 cgcggcatcg ggtgcgacga ttatcgatca ggcgcgcgac ctttttcagc aagccgaaat
    31081 gatcgtgaaa gtgaaggaac cgctcccggc cgaatacggg cttctgcgca agggacagat
    31141 tctcttcact tacctgcatc tggcggcgag tcggccgctg accgaagcgc tcttgaaatc
    31201 tggcgtgacg ggcgtggcct acgagacgat ccaggtgggc aaccgcctgc cactgctcga
    31261 accgatgagc gagatcgccg gacgcatgtc ggtcgtgatg ggcgcctatt tcctcgcgaa
    31321 acacaacggc ggcaacggcg tgctgctcgg cggcgtgccg ggcgtgctcc cgggaaaagt
    31381 ggccgtgctt ggcggtggca catcgggcgt caacgccgca cgaatggcga ctggcctggg
    31441 cgccgatgtc actattctgg aagtggatct cgaacggatg cgtttcctcg acatgacgat
    31501 gcactcggcc aacacgcttt actcgagcga ggcgcatttg caggaattga tgccggcggt
    31561 cgatctcttg atcggggcgg tgctcgtccc gggagcaaaa gcgccgaagc tgatcacgcg
    31621 cgacatgttg cgcgagatgc gcccgggcag cgtggttgtc gatatcgcga tcgaccaggg
    31681 cggctgtgtg gaaacctcgc gaccgacgac ccatctcaac ccggtttatg tcgaggaagg
    31741 cgtaattcat tactgcgtgg ccaacatgcc cgccgcctac gcaaggaccg cgactcaggc
    31801 gctcacgaat attacttacc gttacgtcga attgctcgct gatctcggtc tgaaggaagc
    31861 gtgtgaacgc caaccggccc tgaccggcgg aatcaacacg atggatggcc gcctgacgca
    31921 ccgcgccgtg gccgaagcgc acgggatgga gtacgagccg cctttccaac ccgctcccgg
    31981 aggcgcgttt ccctcctagg cgcagattct ggccgagagg aaagttccat ccgaaaataa
    32041 aaatgccaca gagccgaaaa cttcgcttgc tccgaaccaa tagttacccc aacatggccc
    32101 ttcatccaca tcctatgaag aaatatatct gcattctggc tagcgccgcg cttctcgtag
    32161 cgtgcgaaca aaaaaccgaa accgcggctc cggctgcatc tcccgccgag accacctcgt
    32221 cggagacgac aacgactacg actacgacca cgacgagccc cgctgaatct ccgatggcaa
    32281 ccacggctcc gatggaatca tcgagtccaa tggaagctgc cagcccggct gcgaccgagg
    32341 gcagcaccac ggagacgacc ccggcgccga ctccttagcc ccggcctagc ttcaaaggca
    32401 gaatctttct gcgggccttt gcccggagga agatttttgc ggtacgcgag atgcgctggt
    32461 cggccaatct gagtggctgc cgggaccgct cccatgtttc gccacggaag aggctggcgg
    32521 caatctcatc cgaaatgacc ggtttgctca acaaagcctt ggctactagc cacgccattt
    32581 cagactgaag ggttgaggtt aaaccattcg ctagttcaca gcataagcga tcgtgctgag
    32641 gagcgctgct ccgtcggcga ggtcgaggcg tgtgtctcgc acgacccgtg tcgcgtggga
    32701 tctcaaacga gcgcgaaaat ccaaaccgcc cagataattt ctgggaaccg ctcgacgccg
    32761 agtaggatca cgatgcgcac ggacccttcg atccgcccgc gcgccagcgg agtccgcagt
    32821 tgtggcgcca cgacccatcg gcgctggctt gccgccgggt tgctcgccgg cgtggtgccc
    32881 gcactttttg gccggtgcgc ttttcgttag gccgtctcag agacgcgatt cgccctcacg
    32941 acaaaggcgg agcgcgctgt tcacgagggc aacgtgcgaa aaagcttgcg gaaaatttcc
    33001 cagcagccgt tttttgcggg gcgtgtattc ctcggagagg agcccgaggt cattccgcaa
    33061 atcaagcagg cgctcgaaga gcaggcgcgc gtctttgccg cgcccggtga ggtaatagca
    33121 atcgaccagc cagaaagaac agggaagaaa aacaccctcc gggccgggta agccgtcgac
    33181 tttctcttcg cccggccgat agcgcaggac gagtccgttc tcgagcagct cgcgctcgac
    33241 cgccacgatg gttccaagca cccgctcatc gttaggcgga agaaagccga ccaacggaag
    33301 catgaggatg ctcgcgtcca tcacgtcggc cccgtaggac tgcgtgaacg cgttcttttt
    33361 cttgttgtaa ccgaagcggc aaaccgaacg atggatttta tcgcgcaatt tcctccagcg
    33421 ttcgaggctc tggccaggcg cgcagtgata ctgctcgacg agtttcaccg cgcgatcgaa
    33481 tgcgacccag gccatgactt tggaatgggt gaattgccgg cgcgggccgc gcacttccca
    33541 aatgccttca tcgggctcgt gccactttgt ttcgagaaag cgcagcagtg ccacctgtaa
    33601 gcgccagtcg gcctcgttca tctccagccc acagcggtgc gcttgaaaca tcgcgtcgac
    33661 gacttcgcca aaaacgtcga gctgaaattg gcccgaagcc gcgttgccaa cgcggacggg
    33721 ccgggatttt tcgtagccgt ccagccacgg cagttcgtat tcgtcgagcc gccgctctcc
    33781 gcgcaccccg tacatgattt gtaactgggc gggactgcca gcgatggcgc gcagaagcca
    33841 ttcgcgccat gcttttgctt cttcttcgta gccagcgccg agcagtgcgg tgagggtaaa
    33901 agtcgcgtcc cgcagccaac aatagcggta gtcccaattg cgcacgccgc cgattttttc
    33961 cggcaacgag gtagtaagcg cggcgacaat gccgcccgtc ggcgcgtagg tgagaccttt
    34021 cagcgtaatg agcgagcgca tgactgcgtc tcgccaccga ccggatcgcc cgtagcgctc
    34081 ggcccattgc agccagtaga tttccgtttc ctgaagggca tgccctggat ccacttcctt
    34141 gggcggaagt tcgtgggacg cataccaggt aaggacaaac ggcacgcagt cgccttgcgc
    34201 gacggtgaat tttgccaccg tcttgaggtc ttggccacgc gtcgggatgg gcgtgcgcaa
    34261 aatcagcgca tcaggccccg cgaccgcttc gaggccgcca tgccgctgcc gcacccaggg
    34321 tacgatggag ccatagtcga aacgaatgat caactccatg ttaatcgtca ctctcccctt
    34381 cagcccctcg atgatgcgga cgatgtcggg attcgtcccg cgcggcggca tgaaatcgat
    34441 gaaacggacc gcgccttccg ctgtctccac ctcggtctcc agaatcaaac tgtccccacg
    34501 ataacgccgt gcgcacttct tgattctgcc cttcggcttg aattgccagc agccgttttc
    34561 ttttttgcct aacaaagccg caaaacaggc gccggaatca aaacggggca ggcaaagcca
    34621 gtcgaccgag ccgtcgcgac taacgagtgc gccactctgt gtatcgctaa ggaaagcgta
    34681 gtcctcgatc gacgggttga tctttctttt cctcctcttc atcgagggtg cgatcgggaa
    34741 gttttgaacg gcgctcccca aaacacgcgt cattaattaa cagctcatga aaacacaaat
    34801 cactcgcaaa ctacgccgcc cggctcctgc gcgagctgcg cgattccgtc gcgcgtgaga
    34861 cgaaaaatcg tccactcctc gttcggcctg gcccccagct tgcggtaaaa ctggatggcc
    34921 ggctcattcc aatcgagcac cgcccattcc attcgcccgc acccgcgctc gcgggcgatt
    34981 cgcgcgaggc gcaccagcag ggcgcggccg taacctctgc cacggacttc cggcctaacg
    35041 aaaagatcct cgagataaag tccgcgtcgc cccagccagg tggagaaatt gtgaaagaaa
    35101 agcgcgaagc acaccggcac gccgccctcc tcaccaagta acacttccgc cgccggatgc
    35161 gcgccgaaga gcgcttcctc cagggccgct tccgtcgcca ccacatcctg cggcgcgcgc
    35221 tcgtattcgg ccagctcgcg gatcagggcc aggatggccg gcacgtccgc gcgcgcggaa
    35281 aggcgaatcg agaaatcgga tttcttgatc ccagacaccg gatacgagtt agctcttcgg
    35341 ccgcaaagcg caaccgggcg ttctcgccga tcacggccag gcttcagctt ttttcgccag
    35401 cttctcctgg agaaaggcga gcactgcggc ctcctccgcc cgcaagtcgc caaacgaatc
    35461 tgcgagcgtc tcctcgcggc gtttccgcag gcccgcgatc aagttaccgt ccagataggt
    35521 ttccaacacc gcggggtgaa cgtaacactt gcggcagacc gccggggtgt tgccgagcac
    35581 cctcgcgacc gccccgatcg cattcttgat gtttttcttc gcttgcgatt tgttctcgaa
    35641 gggctcctgg gcctgcagaa ccgtcgcggc catgatcgtg ccggcccacg tgcgaaaatc
    35701 cttcgcggta aactcttcgc cggtcgtctc gcgcaggtat tggttcactt cgtcggaggt
    35761 cacctgatgc tgtcccccct tggcgtcgag acactggaag atttcctggc cgggcaattc
    35821 ctgcagtttt ttgatgatcc gggccacgcg ccggtcttca gtgtcgatgt cgtgcgcctg
    35881 gccgctcttg ccgcggaacc gaaagcggac cttcgctccc ttcacctcga catggcgatt
    35941 ccgcattgtc gttaggccaa acgatttgtt ttcctttgcg tatgcttcat tcccaatacg
    36001 gatcaggctc ctttcgagga gctggaccac ggtcgcgagg actttttccc gcgggagtcc
    36061 gcgtcgcttc aggtcgcgat tgattcgtcg ccggatcccg ggcagcgcct gaccgaaaac
    36121 gatcatcttt tcgtatttgt tctcgtcgcg ctcttcgcgc caacggtcgt gataacggta
    36181 ttgtttgcgc ccgcgctcgt cgcggccggt cgcctggagg tggccattcg gcgatggaca
    36241 tatccagacg tccgtgtagg cgggcggaat cgcgagtcga ttcagacgca tgacgcgcgc
    36301 ttcgtcgtgg atctctcttc cctcggtatc gaagtagaga aacgtccggc cccggcgtcg
    36361 ccgcgtgtaa ccgggacttt catcgctcac gtaacgcagc ccggcctcct ccgccgcttc
    36421 ttgcgggtcg gtgataatct cgaggctggc gatttttttc ctttcctggg acagcttgcg
    36481 agcagtggtg gacacgcttg caatacgcgt gttgcatcgg caaaggtcgc ctcggcacgg
    36541 gcatgaagat tcttgaaacc gaaagattgc tcctctaccc gctgacgacg aaggacgccg
    36601 acttcatgct catgctcatg aatgaacccg gcttcatcaa atacgtcgcg gaccgcggtc
    36661 tgcggacaag cgcggatgcc gcggattaca tttcccaaaa gattattccg agctacgcgc
    36721 gattcggatt cggactttat cgagtggact tgaaggaatc caagacggcg atcgggatct
    36781 gcggattggt gaagagggac acgctcgagg aggtggacat cggattttcc attctccagc
    36841 gattttgcgg taagggttac gcctacgagg cggcggcggc ggtaatgcga tacgggcgca
    36901 acgtccttgg attgcctcgc atcgtcggtg tcaccgcgcc agacaaccgg gcttcgattc
    36961 atttgctgga aaaactcggc ctgaagcggc agcgaaaaat ccatttgcct ggatacgggc
    37021 cggaaagctt gctcttcggc taatcgccat cggcgcctcg acggcaatcc cacgggacga
    37081 ctgcggggac cactggcgaa acgaggccgc agcgcgaatt cgatcatccg tcgcggagcg
    37141 cggcatgggc attgctataa ttgtcatgat tatcataatg aaccatcaag cctttttccc
    37201 gtttgccaaa ctcaatctcg ctctgattgc ttctcttttc ttcctcgccc cggtcccccg
    37261 agcggtccgc gccgaaactg catcgcaaga atccgagccc acggaggtct acgccaccgc
    37321 gtccgacgga accatactcc actgggtcgt ttacacgcca gcgggtgccg gcccctggcc
    37381 tgcggtcctc gtcattcacg gcggcggctt caaaggcgga accccaacca gttcgcccga
    37441 atcggtcagc tgcggacgcg atttggccgc cgctggattc atcgcttttt cgatcgaata
    37501 ccggctggcg ccgccaggat cattgctcgg ccaggtgacc gacggacgtt tccccgacca
    37561 accggatgac gtcaaactcg ctgtgttggc agcgcgcaac gacgcccgct gcaacggtca
    37621 ggtcggttcc gttggcggtt cggccggcgg ctacgagaca gcctttgtcg cgggcgcggg
    37681 aacgatcggg caggaccgga tcgacgtcgg tgtcagcctt tccggagcct acgatttgtc
    37741 agactttagc ccggatcctg acattcaagt ctttgcggac gacgtgacga attacgtcgg
    37801 atgcacaacg cttgataccg ggttgttgcg cgccgcatct cccgcctatc tcgccgacag
    37861 caataccgca ccgctcttca tcgtccacac cgagcaggac ccgatgccct ttagtcaggt
    37921 cgctgatatg acgacccagc tcgatgccct gggtgtgaca aattaccagg ctcttacact
    37981 ggccgggaac cagcattcgt tttcctactg gcctacggtg aaagatcagg cgattggatt
    38041 tctggggtcc tggttcgcgg gcgtccctcc ccctccgctc cccactccca cgccaactgc
    38101 gacgccgacc gcaacgccga cgccaacgcc gtcgccttct ccgaccgcca cgccagatca
    38161 aacggcttcg cccgcaccgc cgccggtgat cgcccctact ccgaccaaaa tgctgcttaa
    38221 cgtctcaact cgggtgcgcg tcgagagcgg gagcagcgtc atgatcgggg gcttcatcat
    38281 caccggcgac gtggccaaga aagtggccat gcgagcgatc gggccatctc tggctgacgc
    38341 tggcgtgagc ggcgtgctcg ccgacccggt gctggagtta tatgactcca ccggaagcct
    38401 gatcgcacaa aatgacaatt gctcttcgct gccgccggac agcatccctg cggatttgaa
    38461 accgaccgac gggcacgaat ccttcatcgc cgtcaccctg cctccaggga gttacacggg
    38521 agtcctgcgc ggagcaaacg gcgcttccgg gataggcctc ttcgagcttt acgatctgga
    38581 tcctgccagc tcgcgtatta gcaatatttc cacccgcggc gacgtcggaa ccggcagcga
    38641 cgtaatgatc ggaggcttta tcatcggcgg cgccgagccg acgaaagtaa ttgtgcgagc
    38701 gatcggtccc tcgctcgtcg ccgagaatat ttcggatgcg ctgcccgatc cactcctcga
    38761 actatacgac agcaacggct cgctcatctt cacgaatgac aactggcgga ccactcagga
    38821 acagcaaatc atcgattcag gcgtcccacc cagcgatgac cgtgaatcgg cgatcgtggc
    38881 cacgctgctt ccgggaagtt acacggcgac ggttcgtgac gctgggctgg ccactggcat
    38941 agccctgatc gaagtctaca accaggagtc aaactaaagt tcttgagcgc cggcggaacg
    39001 caggtgtccc gtccgcgctc ccaggaaaat ggcacacccg cgccagccta ggcaccgtag
    39061 gtcttggtga acttcttttt cgactctgtc tcgggttcgg taacggcgga cggtggcgcc
    39121 tcgcccggcg gcgagccagc tgcgggagcg ccgggttcaa cgtcgggcgc tgctcccgaa
    39181 gcgggcgccg gcgtccgtcc cggcctcccg ctctgcgatg cctccacaaa agccatctgc
    39241 aggctcgaga gtgcattgcg cagcacggtt gattcttcgc tcgttaggtt gccgcgggtc
    39301 ttttcctgaa tcatcgccaa ttggtcgatg aagaggcgag cgacctccag gttgacctcc
    39361 gcctggccgg tctgcgggtt gggaatctgg ccgagaaaaa gtgcggcatt ttgcgcctgc
    39421 atcatgacga actcgatgaa gcgctgagtc aattctccgg actgggtggt ggtttggact
    39481 tcggccataa tgagtagtga ggttgaggag ttaggttagc aacggttaaa tgtgggacgc
    39541 tggcctcacc gtagcgagag cgtaaggttg ttgaaaatat tttgctgcca ccctttgcac
    39601 gtcgtctggc gtgaccagcc cgacctcgtc ttccagcttc cggtagtggt caaaaccgag
    39661 gccatagagc tcgtcgagcg ccaccatgta agcgaaggag tcgttgcttt ggttggcaat
    39721 tcgttgctgg ccgatgagct ttttcttcgc ccgcgtcaac tcttcgttcg ttaggccggc
    39781 agcggccagc tttccaatct catcgagaag cgcggccgtg accggcttga gcttggcggg
    39841 gtccgtcccg agataaaagg cgaacaaccc cggaaccagt ccctgcatct ggcttgtgcc
    39901 cacgtaatag gccagcccca tctcctcgcg gatgcggacg aaaaagcgcg atccgagatc
    39961 gctgctcgct tcgtcgatca gctccagcgc gtagcgatcc gggctaaaca ggccggcgcc
    40021 acggtacccg accatcagga cgccctgagc cttctctttc acttcctcga ccgcgatcgt
    40081 cttcgcgagc cggggcggtg acggcgcgtc ggtcagggcg agttcacccg caggcatcgc
    40141 gctgagtatt tgctcgaaaa gttgcttcac ctcagcggcc ttcacactgc cgaaaacaga
    40201 gatgacgcca ttcttcgcca cgagataacg cgtgcggaaa gcgagcagat ccttctgcgt
    40261 aagctgtgcg actgcttccg gtgtcccttt cgcccgcagg gcgtagggat gctggccgaa
    40321 aagcgcggcc cgcaggatgt tgcgcgcgac cgttgtcagc tgttcttcgt catcgcggag
    40381 accggcgagc tgcacttcct tttcccgggc gacggctttt tcgggcatcg tggcgttgag
    40441 caaaatgtcg ccaagcacct ccgcgccgag tcgcagatcg ggtttggtga cctcaagcga
    40501 aatgttgaag ctgttattgc ccgcatcgct cccgagggtg ccgccgacgg cctcgatcgt
    40561 gtcggcaatc tgctcggcgg tgcgggtttt ggttcccttc accaacacct tggccatcaa
    40621 gcgggtcagg ccgctggtcg cccgcgtctc ggcgagcaaa cc
//
LOCUS       DATMNS010000209         3650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1012403, whole genome shotgun sequence.
ACCESSION   DATMNS010000209 DATMNS010000000
VERSION     DATMNS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1012403"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VNW28_07260"
     CDS             complement(<1..559)
                     /locus_tag="VNW28_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXA09765.1"
                     /translation="MTKPLYALILAGGSGERFWPLSRRARPKQLLKLVSEKTLLEETI
                     DRLAGLVPNERILILTNVEQEAAVRELLPGLPPENIVAEPSKRDTAAAVALATAWVAA
                     RDHRATMLVLPADHIIKDQAAFQKTMSTAARAAETTGALVTIGIKPTWACPGFGYIEQ
                     GAPAPLPGGSAPNDIFRVVRFREKPN"
     gene            642..944
                     /locus_tag="VNW28_07265"
     CDS             642..944
                     /locus_tag="VNW28_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09766.1"
                     /translation="MSISLSIPKTAETLPLDGLLTLTQAAFGLGLGLLIAERLNRPAR
                     QATAIALVSLGTLAVIPLLVKKAMERINHPASDRVMRRRLRSIRGDSGYGSERDIY"
     gene            1105..2739
                     /locus_tag="VNW28_07270"
     CDS             1105..2739
                     /locus_tag="VNW28_07270"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HXA09767.1"
                     /translation="MTARPDSSTRNALRERCEAILQEEEILREGGGKVGQDRQRKMGR
                     LPARERIAHLLDRGADFFEIGLWAAYKMYEQWGSVPAAGAVAGIGQVAGIPCMIIAND
                     ATVKAGAFFPQTAKKLIRAQRIAFECALPIIYLVDSSGVFLPMQDEIFPDEDDFGRIF
                     RNNSVISAAGLPQFAAIMGNCVAGGAYLPVLCDKVLMTEGSGLYLAGPSLVKAAIGQI
                     VDAEELGGALMHAEVSGTVDFHEKDDPSCLKRLRSLVAHLPEAEAAKKNGAEKKPTAP
                     ANDPHAVYDLIDLEGQKQYDARDLLASVIDTDSLDEYKADYGKTLVTTYARIGGHSVG
                     IVANQRHQVRTKKAGIQMGGVIYSDSADKAARFVMDCNQTGLPIIFFQDVTGFMVGRD
                     AEQSGIIRSGAKLVNAVSNSIVPKITIVVGGSFGAGNYALCGKAYDPRFILAWPNARY
                     AVMGAAQASDTVFSVLARSRERGDKKATPEELEQLRAKVKETYEEQTDIRYGAARGWL
                     DAIIQPHETRDVLMKLLGYVARPMPKARFHMGVVQT"
     gene            complement(2845..3591)
                     /locus_tag="VNW28_07275"
     CDS             complement(2845..3591)
                     /locus_tag="VNW28_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXA09768.1"
                     /translation="MAFNNFPGQIGSRPVTAPGQLSGNLASVYVKDTLINIAARHIEG
                     VDFSASYDWDLHRYGQAQLGVNAVMFTLNETKTYPQSAYYNINGLDFPEGGGANPNYR
                     ITALARYSYEGASAAINMNYLPGLDNAQGRDPEHEDQFSFMKIGDYLTFDMRLQYEFR
                     AKPEAAAPSYSQDAKDSKGIVAGANSVAAPERTINPFSRLVDGLTVAFGCNNIFDRVP
                     PTVDGANNNTDLSVYDPYGRFLYFEVSKKF"
BASE COUNT          716 a         1097 c         1111 g          726 t
ORIGIN      
        1 cgttcggttt ttcgcgaaaa cgaaccaccc gaaagatgtc gttaggcgcg gagccgcccg
       61 ggagcggggc gggcgctccc tgctcgatgt agccgaaccc cgggcacgcc caggtcggct
      121 tgattccgat cgtaacgagc gcacccgtcg tctcggcggc gcgcgcggcg gtgctcatgg
      181 ttttctggaa ggcggcctgg tctttgatga tgtgatccgc gggcaggacg agcatcgtgg
      241 cccgatgatc gcgggccgcg acccacgccg tggcgagcgc caccgccgcc gcggtgtcgc
      301 gtttcgacgg ttcggccacg atgttttcgg gcgggagacc gggaagcaac tcccgcacgg
      361 ccgcctcctg ctcgacgttc gtcaggatga gaatccgctc gttagggacg agccctgcga
      421 gacggtcgat cgtttcttcg agcaaggtct tttccgaaac cagtttcaac aactgcttgg
      481 gccgcgcccg ccggctgagc ggccaaaaac gttcgccgct acctcccgcc aggatgagcg
      541 cgtagagcgg tttcgtcata gggaaaaaat gagcgagtga ggatctgacc gaagattttt
      601 cccgtttctt ttttcagcga cgggcgctat aaggttaggc gatgtccatt tcgttatcca
      661 ttccaaaaac ggcggaaacg ctgccgttgg acggactctt gaccctgacg caggcggcct
      721 tcggtctcgg cctcgggttg ctcatagccg aacgcttgaa ccggcccgcc cggcaggcca
      781 cggcgattgc gctcgtctcg ctcggcactc tcgccgtcat tccactcctc gtgaaaaagg
      841 cgatggaacg gattaatcac cctgcttccg atcgcgtcat gcgacggcgc ctgcgttcga
      901 tccgcggaga ttcgggttac ggatccgagc gcgacatcta ctaagaattc cggtgggcgt
      961 ttcgctctac gaaacgccgc cctccgcgtg gatcgcggat tgaccacgac atttttccct
     1021 cgcgcttcgc gaagcgaagc ctccccaaca tgcgccgctc gagccttgct tgatcgcccg
     1081 gcttcgccga agtaagtcca cccgatgacc gcccgtcccg actcctcgac ccgcaacgcc
     1141 ctgcgcgaaa ggtgcgaagc cattctccag gaggaagaaa tcctgcgcga gggcgggggc
     1201 aaggttggcc aggaccggca gcggaaaatg ggaaggcttc cggcccggga gaggatcgcg
     1261 cacctgctcg atcgcggtgc ggactttttc gaaattgggt tatgggccgc ctacaagatg
     1321 tacgagcaat ggggcagcgt ccccgcggcc ggcgcagtcg ccggcatcgg ccaggtcgcc
     1381 ggcatcccgt gcatgatcat cgcgaatgat gcgacggtga aagccggcgc cttttttccc
     1441 cagaccgcca agaagctcat ccgcgcgcaa cggatcgcct tcgaatgcgc gctgccgatc
     1501 atctacctgg ttgattcctc gggcgttttc ctcccgatgc aggacgaaat ttttccggat
     1561 gaagacgact tcggccggat ctttcgcaac aactccgtca tctccgccgc gggccttccg
     1621 caattcgcgg cgatcatggg caactgtgtc gcgggcggcg cgtatctgcc ggtgctctgc
     1681 gacaaggtgc tcatgaccga aggcagcggt ctctaccttg ccgggccgtc gttagtcaaa
     1741 gccgccatcg gccagatcgt cgacgcggag gagctcggcg gcgccctgat gcatgcggaa
     1801 gtcagcggca cggtcgactt ccatgagaaa gacgatccgt cttgcctgaa gcgtttgcgc
     1861 tcgctcgtcg cgcatctgcc ggaagccgag gccgcgaaga agaatggcgc ggagaaaaag
     1921 cccaccgctc cggccaacga cccccatgcg gtttacgatt tgatcgacct cgaaggccag
     1981 aagcaatacg acgcccgcga tctgctcgcc tcagttatcg atacggattc gctcgatgaa
     2041 tacaaagccg attatggcaa gacgctcgtc accacctacg cccgcatcgg cggacattca
     2101 gtcggcatcg tggccaacca gcgtcaccag gtccgcacga aaaaagccgg aatccagatg
     2161 ggcggcgtca tctactccga tagcgccgac aaggcggcgc gcttcgtcat ggattgtaac
     2221 cagaccgggt tgccgatcat ttttttccag gatgtgaccg gcttcatggt ggggcgcgat
     2281 gcggaacaaa gtgggatcat tcgcagcggc gcgaagctcg tcaacgcggt cagcaattcg
     2341 atcgtgccga aaatcacgat cgtggtcggc ggttcctttg gtgcgggcaa ctatgcgctc
     2401 tgcggcaagg cctacgatcc acgtttcatt ctcgcctggc cgaatgcgcg ctacgccgtg
     2461 atgggcgcag cgcaggcctc cgacacggtt ttctccgttc tcgcgagatc gcgcgaacgc
     2521 ggcgacaaaa aagcgacgcc cgaagagctg gagcagttgc gcgccaaggt gaaggaaacc
     2581 tacgaagagc aaaccgacat tcgttacggc gcggcgcggg gctggctcga cgccatcatc
     2641 cagccgcacg agacgcgcga cgtgctcatg aagttgttag gctacgtcgc ccgcccgatg
     2701 ccgaaggctc gtttccacat gggcgtggtc cagacctagc acggcgaagt cgcgccgaag
     2761 agacggcgct tcggcctcat ctagccatca tgaattgaga aatgagcgcg aatcacagac
     2821 caacactttt tcctacccta acgtttagaa ttttttagac acctcaaagt aaaggaaacg
     2881 gccatagggg tcgtaaaccg agaggtcggt attattgtta gccccatcaa cggtgggtgg
     2941 cactctatcg aagatgttgt tgcatccaaa agcaactgta agcccgtcca ccagacggct
     3001 gaagggattg attgtcctct cgggcgccgc gacactattt gccccggcca cgattccctt
     3061 ggagtccttg gcatcttgcg agtaggacgg cgcggccgcc tcgggcttgg cccggaactc
     3121 atattggagc ctcatgtcga aggtgagata atcgccgatt ttcatgaacg agaactggtc
     3181 ctcgtgctcc ggatcgcgac cctgtgcgtt gtccaagccc ggaagatagt tcatattgat
     3241 tgccgcgctc gctccctcat aactgtagcg ggccagggcg gtgattctgt agttcgggtt
     3301 ggcgccgcct ccctcgggaa agtcgaggcc attgatattg tagtaggcgc tctgcgggta
     3361 tgtcttggtt tcattcaggg taaacatgac cgcgttgacg cctaattgcg cctggccata
     3421 gcggtgcaga tcccaatcgt agcttgcgct gaagtcgacg ccttcgatgt ggcgcgcggc
     3481 gatgttaatc agcgtgtcct taacgtagac cgaggcgagg ttgccagata actggccggg
     3541 cgctgtcaca ggtctggatc cgatctgacc tgggaagtta ttgaaggcca caagattggc
     3601 ataaggttac gctggaccga gggcttgcac gctgtcaaga atgatagtgg
//
LOCUS       DATMNS010000210         4753 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5306254, whole genome shotgun sequence.
ACCESSION   DATMNS010000210 DATMNS010000000
VERSION     DATMNS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4753
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5306254"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2538)
                     /locus_tag="VNW28_07280"
     CDS             complement(<1..2538)
                     /locus_tag="VNW28_07280"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HXA09769.1"
                     /translation="MFSLALGCSLGLISVDGARPAKTKAASPSPTPAPAAAAAPSPTP
                     ANAAEPKGENKAQFDGKLWSGMKWREIGPFRGGRAIAIEGVPGEPDTYYFGGVAGGVW
                     KTTDGGGNWAPVFDKQHSTSSIGAIAVAPSDHNTIYAGAGEPALRGNITYGDGVYKSV
                     DGGKNWRNVGLKDSRHIGALIVNPDNPDIVFVAAVGHAFGPNAERGIFRTTDGGKTWT
                     KVLSKDENTGAIDVVFDPHNPNTLFAALWQARRQPWFFSSGGEGSGLYRSIDGGTTWQ
                     QLKGHGLPDGILGRIGVSVSGGDSDRIYAVIEAKEGGIFRSEDGGDNWTRINDDLRFR
                     QRAWYFSKIYADPRAPDTVYVLNTGLFRSVDGGKTFNLLPARHGDHHGLWIDPKNPDR
                     IANASDGGASISIDGGQTWTTENNQPTAQFYHVAVDNAFPYHIYGAQQDNSNVGIASR
                     SESGVIGREDWFQAGGGECGFVVPDPRDWHIIYSNSEGYITRYDKSKEQSQDISVWPL
                     DHSGHGASDLKHRFQWVSPLLLSPHDPETIYTAGEAVFKSTDHGESWTAISEDLTRND
                     KSKQQPSGGPIQNDITSVEYYDVVFALAESPLKKGMLWAGTDDGLVHVTTDDGATWAR
                     VTPNMPEWSLVSLIDPSPFDAAGAYVAVDRHRLDDFKPYIFKTTDTGRNWTAIVNGIP
                     DGAYVHAVREDPKRRGLLYAGTELGVYVSFDDGGHWQPLQMNLPTSPIHDLVVKDDDL
                     VVATHGRSFWVLDDLTPLRQVNAQLAQADMTLYQPQTAVRLHYPGEIDSRQPAGTNPP
                     SGAIIDYYFKEAPKGEVTLEILDAHGKPIRRLSSKENKEDEQPPEWPD"
     gene            complement(2694..3248)
                     /locus_tag="VNW28_07285"
     CDS             complement(2694..3248)
                     /locus_tag="VNW28_07285"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HXA09770.1"
                     /translation="MEIVFFPTPADLRKWLRQNHFTARELWIGFYKKHTEEQSITWPE
                     SVDEALCVGWIDGIRKRIDDASYMIRFTPRRPGSVWSAVNIRRAELLTREKRMQPAGR
                     AAFAARRENKSGIYSYEQRSKELPKPYASTLRKNRDAWKFFQAQPPSYRKTVGWWVVS
                     AKREETRRRRLQKLIMESARRKRL"
     gene            complement(3321..3671)
                     /locus_tag="VNW28_07290"
     CDS             complement(3321..3671)
                     /locus_tag="VNW28_07290"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXA09771.1"
                     /translation="MRPLHHPAARDISVEGILYALSDPVRMQIFAEIEGADCAQTCSN
                     FLIVAKKALPKSTLSQHFKILREAGLIRSERKGVEMHSTTRCAELKERFGPMIREIIA
                     AYRGKSGRPKGRKR"
     gene            3794..4540
                     /locus_tag="VNW28_07295"
     CDS             3794..4540
                     /locus_tag="VNW28_07295"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXA09772.1"
                     /translation="MNKLSNKVAVVTGASKGIGAAIAQALAAEGAAVVVNYASSKAGA
                     EAVVAAITNAGGKAIAVGGDVSKAAQAQGIVDAAIKNYGRLDILVNNSGVYEFSPIED
                     FTEEQFHRLFNINVLGLLLTTQAAIKHLGEGASIINIGSGVTRITPPNTAVYSGTKGA
                     VDAITGVFAREFGPKKIRVNSINPGIVQTEGTTSAGFIGSDFEQALVAQTPLGRAGQP
                     GDIASIAVFLASDDSAWLTGERLIASGGLR"
BASE COUNT          875 a         1477 c         1428 g          973 t
ORIGIN      
        1 atcgggccat tccggcggtt gttcgtcttc cttgttctcc ttgctcgaga gacggcgaat
       61 cggcttgccg tgcgcgtcga gaatctccag ggtcacttcg cctttcggtg cttccttgaa
      121 gtagtagtcg atgatcgcgc cggaaggcgg attggtcccg gctggctgcc gcgaatcgat
      181 ctcgcccgga taatggagac gcaccgcagt ctgcggttgg tacaaggtca tgtcggcttg
      241 cgcgagctgc gcgttgacct ggcgcaacgg cgtcaaatcg tcgagcaccc agaaggatcg
      301 gccgtgtgtc gccaccacga ggtcgtcgtc tttcaccacc agatcgtgaa tcggcgaagt
      361 cggcagattc atctgcaacg gctgccaatg tccgccgtcg tcgaaggaaa catagacccc
      421 aagttccgtg ccggcataga gcagcccgcg ccgcttggga tcttcgcgca ccgcgtgcac
      481 gtaggcgccg tccggaatcc cgttgacgat cgcggtccag ttccggccgg tgtcagtcgt
      541 cttgaaaatg tagggcttga agtcatccag ccgatggcga tcaactgcga cgtaggcgcc
      601 ggcggcatcg aagggcgagg gatcgatcag actaacgaga ctccattccg gcatgttcgg
      661 cgtgacccgg gcccaggttg cgccgtcatc tgtcgtcacg tggactagcc cgtcgtccgt
      721 cccggcccag agcatccctt ttttcaaggg cgattccgcg agcgcgaaaa caacgtcgta
      781 atattcgaca ctcgtgatgt cgttttggat cgggccgccg ctgggctgct gtttgctctt
      841 gtcgttgcgg gtcaggtcct cgctgatcgc ggtccagctt tcgccgtgat cggtggattt
      901 gaaaacggct tccccggcag tgtagatcgt ctccggatcg tgtggcgaaa ggagaagcgg
      961 tgagacccac tggaaccgat gtttcaaatc gctcgctcca tgcccggaat gatcgagcgg
     1021 ccagacactg atgtcctggg attgctcctt cgatttgtcg tagcgcgtga tgtagccctc
     1081 gctgttggaa taaatgatgt gccagtcgcg cgggtcgggc acgacgaagc cgcactcgcc
     1141 gccgccggcc tggaaccaat cttcgcgccc gatcacaccg ctttcgctgc gactcgcgat
     1201 cccgacgttc gagttgtcct gctgcgcgcc atagatgtga tatggaaacg cgttgtccac
     1261 cgcgacgtga taaaactgcg cggtcggctg attgttctcc gtcgtccagg tctggccgcc
     1321 atcgatcgag atgctggcgc cgccgtcgct cgcgttggcg atgcggtcgg gatttttcgg
     1381 atcaatccag agcccgtgat gatcgccgtg acgcgcggga aggagattga acgtcttgcc
     1441 gccatcgacc gaacggaaga gtccggtgtt cagcacatag accgtatcgg gcgcgcgcgg
     1501 gtccgcgtag attttgctga aataccaggc acgctggcgg aagcgaagat cgtcgttaat
     1561 cctggtccag ttatcgcccc catcttccga gcggaaaatc ccgccttcct tcgcttcgat
     1621 gacggcgtaa atccgatcgg aatctccacc cgaaacgctc acgccgatgc gcccgaggat
     1681 gccgtccggc aaaccatgtc ccttgagttg ctgccaggta gttccaccgt cgatcgaacg
     1741 ataaagcccg ctgccttcgc cgccgctcga gaaaaaccag ggctgccggc gggcctgcca
     1801 gagcgccgcg aagagcgtgt ttggattatg cgggtcgaag acgacgtcga tcgcaccggt
     1861 gttttcgtct ttgctcagga ctttcgtcca cgtcttgccg ccgtcggttg tgcgaaagat
     1921 cccgcgctcc gcatttggcc cgaaggcgtg cccgaccgcc gccacgaaaa cgatgtccgg
     1981 attatccgga ttgacgataa gcgcaccgat atggcggctg tctttcaggc cgacgttgcg
     2041 ccaattcttg cccccatcga ccgatttata aaccccgtcg ccgtaagtaa tattaccccg
     2101 cagcgccggt tcgccggctc cggcataaat cgtgttgtgg tcgcttggcg cgacggctat
     2161 cgcgccgatc gaagaagtcg aatgttgctt gtcgaagacc ggcgcccaat tgcctccgcc
     2221 gtcggtcgtt ttccagactc cgccggcgac tccgccgaaa taataggtgt ccggctcgcc
     2281 tggaacccct tcgatcgcaa tcgcccgacc cccgcggaag ggtccgatct cgcgccactt
     2341 cattccactc cagagtttgc cgtcaaactg cgctttgttt tcacctttcg gctcggccgc
     2401 gttggccgga gtcgggctcg gggccgcggc cgcggcgggc gcaggcgtgg ggctgggcga
     2461 ggcggccttc gttttggccg gcctcgcgcc gtcgacgctg atgagaccaa gggagcaacc
     2521 gagagccaaa ctgaacaaca gcgcacgctc tacgctcctg aaatgattaa gcatcgcaca
     2581 gtgtggaact gttcggctgg ccttcaactg gaatgtcttt ccagccggac gagcaacttt
     2641 ttccgtcggc ccgcgccacc gccccacggg ccgcaagggg cagggcagga aggtcagaga
     2701 cgttttcggc gggctgattc catgatcaat ttctggagtc gtctgcggcg cgtttcttcc
     2761 cgtttcgcgc tcaccaccca ccagccgaca gtttttcggt aagagggtgg ctgtgcctgg
     2821 aagaatttcc aagcgtcccg gttcttccgc agcgtgctcg catatggctt gggcagctcc
     2881 ttgcttcgct gttcgtacga atagatgcct gatttgtttt cccgtcgcgc ggcaaacgcc
     2941 gcacgtccgg ccggttgcat tcgcttctcg cgagtcagca gctccgcccg tcggatattg
     3001 acggcgctcc aaacgctgcc tggccggcgc ggcgtgaagc gaatcatgta gctcgcgtca
     3061 tcgatccgtt tccggatgcc atcgatccag cccacgcaaa gcgcttcgtc caccgattcc
     3121 ggccaggtga tgctctgctc ctcggtgtgc ttcttgtaaa agccaatcca gagttcccgc
     3181 gccgtgaaat gattttggcg aagccatttc cgcagatcgg ccggggtagg aaagaagacg
     3241 atttccatat cgcagctcgc tgcgatgtta ttctgcgcaa gagcgcagga tcgcgccaac
     3301 aactttcgcc ccgagggccg ttaacgcttc ctgcctttag gccggccgct cttcccgcgg
     3361 taagcggcga taatctcgcg gatcatcggg ccgaagcgct cctttaattc ggcgcatcgg
     3421 gtggtgctgt gcatctccac tcccttgcgc tcgctgcgga tgaggccggc ttcgcgcagg
     3481 atcttgaaat gttgcgagag ggtggatttg ggcagggcct ttttggccac gatcagaaaa
     3541 tttgagcaag tctgcgcgca gtccgcgcct tcgatctcgg caaagatctg cattcgcact
     3601 ggatcggaga gcgcgtagag gatgccctcg acgctgatgt cccgggcggc gggatggtgg
     3661 agtggtcgca caaatattcc ttagcaccga cttgacaaat agtccattag ttcacatgtt
     3721 gtgaactagt gaacaaaacg gcggcgctgc ccccggcgcg gcgattcgcc aaacaaccaa
     3781 ccaaacaaaa ttcatgaaca aattatcgaa caaagtcgca gtcgtcacgg gagcctcgaa
     3841 aggaatcggg gccgccatcg cccaggcact cgccgccgaa ggtgccgcgg tcgtcgtcaa
     3901 ctacgcctcg agcaaagccg gggcggaagc cgtcgttgcc gccattacca acgccggggg
     3961 caaagcaatt gccgtcggcg gtgacgtttc gaaggccgcc caggcccagg gaatcgtcga
     4021 cgcggcgatc aaaaactacg gtcgcctcga catcctcgtg aacaactctg gcgtctacga
     4081 gttcagcccg atcgaagact tcaccgaaga gcagtttcac cggctgttta acatcaacgt
     4141 gctcggactc ctgctcacta cccaggccgc gatcaagcat ctcggggaag gcgccagcat
     4201 catcaacatt ggctccgggg tcacccgcat cactcctccg aacaccgcgg tttactcggg
     4261 aaccaaaggc gccgtggacg ccatcacggg tgtgttcgcg agggaattcg ggccaaagaa
     4321 gatccgcgtc aattcgatca accccggcat cgtccaaacc gaaggtacga cctccgccgg
     4381 cttcatcggc tctgacttcg agcaggccct cgtcgcgcag accccgctcg gccgcgccgg
     4441 ccaaccgggc gacattgcct ccatcgccgt ctttcttgcc tcggacgact ctgcctggct
     4501 caccggcgag aggttgatcg cgagcggcgg tcttcgctaa tatcggaagg acgcaggttg
     4561 ccgccggggg ctgcaacggg cagttcccgg gaaatggggt cgccaaacag atatttagtg
     4621 gaccgtctct cccgggaagc aggcggagcg cccgctctgc aattcgggat cacgatggct
     4681 cggcggtgca gcggacgcgg cgggccgcgt agtaggaccc cagcacgacg agcgcggcga
     4741 tcgcctgcgc gat
//
LOCUS       DATMNS010000211        18069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1727457, whole genome shotgun sequence.
ACCESSION   DATMNS010000211 DATMNS010000000
VERSION     DATMNS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 18069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18069
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1727457"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VNW28_07300"
     CDS             <1..225
                     /locus_tag="VNW28_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dockerin type I domain-containing protein"
                     /protein_id="HXA09773.1"
                     /translation="VGNHSNTVLSPQWGLLIGDTTGDGTVNAGDIGQTKSQSGQPVTA
                     SNFREDVTVDGSINAGDIGLVKSKSGTGLP"
     gene            complement(560..748)
                     /gene="thiS"
                     /locus_tag="VNW28_07305"
     CDS             complement(560..748)
                     /gene="thiS"
                     /locus_tag="VNW28_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXA09774.1"
                     /translation="MNGERAESEDGGTVHDLIQRLALAPETTLVELNGAALRRREWAT
                     QNLRENDRLEFLRVAAGG"
     gene            complement(757..1875)
                     /gene="thiH"
                     /locus_tag="VNW28_07310"
     CDS             complement(757..1875)
                     /gene="thiH"
                     /locus_tag="VNW28_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HXA09775.1"
                     /translation="MSFAPILDQLPPAQSPLLRRFASLLEPNDEAVFERMARQARELT
                     GQNFGRTMRMFAPLYLSNECINNCRYCGFSRDNPILRVTLTVEQVVAEARHLAGQGFR
                     QVLLVAGEHPKFVSGSYLADCIRALVPLFPSISLELGPMETAEYKPLVEAGAEGLVVY
                     QETYHRGVYAEMHTAGPKRNFNWRLDCPERASASGFRRIGIGALFGLAPWRPEALSLA
                     AHLEYLLRHCWQSNLTVSLPRLRPAAGGFVPPFSLGERELAQLVCAFRICFPQVGIVL
                     STRERAPLRDALASLGVTIMSSGSHTEPGGYTGQGRHAVHQTVRGRILPPDAGIDALA
                     TGQFEVSDERSPAEVASVLRRRGFEPVWKDWDQALSTR"
     gene            1940..2611
                     /locus_tag="VNW28_07315"
     CDS             1940..2611
                     /locus_tag="VNW28_07315"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXA09776.1"
                     /translation="MTKPKFIPLPPREFLSDDEVLRRAADFHLEMARRRTVRDFSDRP
                     ISRAVIENCLRTAGSAPNGANLQPWHFVAVSDPGLKHEIRVAAEAEEKEFYEHRAPNE
                     WLEALAALGTNAHKPFLEIAPWLIAIFAQPYRLLPNGQRMKHYYATESVGIATGLLVA
                     AVHRAGLVSLTHTPSPMSFLNKLLHRPAHEKPFLLLVVGHPAAAAVVPDIERKPLAEI
                     SSFIG"
     gene            complement(2618..3217)
                     /locus_tag="VNW28_07320"
     CDS             complement(2618..3217)
                     /locus_tag="VNW28_07320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09777.1"
                     /translation="MKKFIVSLFAVMMFVAGSQAGPVETTGKEAQQSTSQSTEDWYRN
                     REWNIDLFGAYAFTGEPYRTDRYLDADHAWGGGIDANYMFSRYLGVGVEGFLLDLNDV
                     VGQASANLIFRYPIPNTRIAPYAYAGGGAIFNGSRVEDLVARDFRHDGDTEGMGQFGA
                     GFEVRVTPHIGIINDFSWNVVNGDHNNFGMVRSGLRFAF"
     gene            complement(3413..4987)
                     /locus_tag="VNW28_07325"
     CDS             complement(3413..4987)
                     /locus_tag="VNW28_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXA09778.1"
                     /translation="MIEHVKRILGLSRRKTGNKLIISVEKLERRVALLEDGQLEEYSV
                     ERDSSRNIAGSIYKGRVKNIEPGLKAMFVDIGLEKNAFLHFWDAIPAALDSGIEEIER
                     PDQKRSKKRLVARDIPEIYPVGSEVIVQVTKGPIGTKGPRVSTNLSFAGRYLVLMPFS
                     DRSGISRKIEDPKERARLRKILRELEIPDGIGVIFRTVGEGQRARYFVRDLRFLLEQW
                     SKIEQKIKELPAPARVFEEPDLVDRTVRDFLTDEIDEVLCDDHAAVERMAQMVGEISR
                     RAKSRIKYYDGAAPIFETFGVQKQIDDAFHRQVWLKCGGYIVIDETEALVAVDVNTGR
                     NKGGRDVEKTILQTNLEAADEIARQLRLRNIGGLIIGDFIDMKNRRDQQLVYSRMRER
                     LKRDKAKTHVLPLSSLGLMEMTRQRAQESLSDSIYQNCPYCEGRGVVKTTMSTSVEVH
                     RALNAVMRKYQDSIHDIRVILNPEVLKRLKEEDEELLVELERRYAGRLMFRADPTFHH
                     EKFIVTDANTGAELKT"
     gene            complement(5049..7322)
                     /gene="mrdA"
                     /locus_tag="VNW28_07330"
     CDS             complement(5049..7322)
                     /gene="mrdA"
                     /locus_tag="VNW28_07330"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXA09779.1"
                     /translation="MRRAKPALRLQFLGLCMLLGLGALVLRLWWVQVARGYEWTARVQ
                     SSSEATVRIPSIRGEIRDRNGVALVQNRASYEVDFYLPEMVKGYRQRYGRPPETRYRA
                     TISGMPKDLKEPDIVRIVNDGVVPRLEDLDLARDYNANQLQKHYRNDTEVPYSYIKDI
                     DFETMAKFSEHDVGLPGVDIAIKPVRSYVYGALASHLLGYVGAPNDTNTEQAKKYTFY
                     QGDAEGKSNIEKTMDEFLRGQPGIRVMRRNAKGTIDGLLREEPPKQGANVYLTLDARI
                     QAIAEEALRAVSRAGAVVVDPNNGNILAMASVPSFDPNTFIPSIKAKDWQALQKDEGD
                     PLVNRAISALPPGSTFKLITSFAGLRGPKDLSNAKYNCGGGVSYGDHFFRCWVAEKHY
                     THGTIGLADAIKVSCDSFFYQYGNAAGIQSIDVVGKMLGIGEESGLQLSGEQTGNMPG
                     PEWMQLHHPRERWTQAQTANVSIGQGYTLVSPLQLAMAYAAAANGGTCYYPRLVDKVL
                     NQDGSPMLDEHGKPAVSQTPRVRSDLRQEVSPQDIDLVRQGLWKVVNEDGGTGGRARL
                     KDVVVAGKTGTAQATDRGHEENVAWFACFAPFDHPKYVVAVMVQGASGHGGEVAGPIA
                     TRILERTLAQDAGKFDMHVAWLAPAHKANPLQLIKAVSYTGGNLGSEDEEGADTSQSA
                     VVQLANDNASPDVEPEADARGKVQNAQQPKRALRALPVATPAPPRPRNFFERLFNSRP
                     RPAAPTPTPAKRARLAY"
     gene            complement(7309..7866)
                     /locus_tag="VNW28_07335"
     CDS             complement(7309..7866)
                     /locus_tag="VNW28_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09780.1"
                     /translation="MIFFLVLVALVFVAQIAEIFIPPLEWMFNAHVYIVPVIVFYGAM
                     ALPLPLMLALAFIAGFLWDAMTVQVLDAHVEISFGWSILLYAVLAAIMHGLKPLFNRG
                     RWEVHCVLSGVCTSLILLAQYLMISFRRGSLIFNHEVWWQIGGPGLLAMLMAPLVFWS
                     LDWISRLTNNPYVPREEGFDEYASR"
     gene            complement(7997..8800)
                     /gene="mreC"
                     /locus_tag="VNW28_07340"
     CDS             complement(7997..8800)
                     /gene="mreC"
                     /locus_tag="VNW28_07340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HXA09781.1"
                     /translation="MSRSSIVAIVLFAAALLALLNIGPQTTRKIQATFFQVISPFLKT
                     GSGLERQISSVRAGLKSLEELERDNAALQVENRSLKATNEALRDVEHEVNRLRHALNY
                     RQRSLFRLVPAEVVSRDSSTWWRTVTINRGEEDGLAPEMPVLTDEGLVGRTTTVSANI
                     SVVLLVSDENCRVAASVEGTREQGIVTGERVAGSLTPRLDLNYLSKQAELKPGQKVYT
                     SGVGGVFPSGLFVGTVRSFEVRALDSQAKLAPAVDLAKLEDVFVVIGRK"
     gene            complement(8867..9907)
                     /locus_tag="VNW28_07345"
     CDS             complement(8867..9907)
                     /locus_tag="VNW28_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HXA09782.1"
                     /translation="MLNRLFGWFSSDIGIDLGTANTLVYVKDQGIVLREPSVVAVQAG
                     TMKVLAVGDEAKRMLGRTPANIVAVRPLKDGVIADFEVTEAMLRHFITKVHNSRVRAR
                     PRVVIAVPSGITEVEKRAVRESATHAGAREVYLIEEPMAAAIGVGLPVQDPAGNMIID
                     IGGGTTEVALISLSGIVFSRSVRVAGDELDEAIVQYMKRAYNLMIGERTAEEIKIKIG
                     SAYPSDKETSVEVKGRDLVAGLPKTLTITSQEVREALLEPISTIVDSVRITLERCPPE
                     LSADLVDRGLVLAGGGALLRGFDRLLSEETGLPVHVAEDPLSAVAEGTGKCLNEIKFL
                     RQVATADRQWRQ"
     gene            10182..10868
                     /gene="rpe"
                     /locus_tag="VNW28_07350"
     CDS             10182..10868
                     /gene="rpe"
                     /locus_tag="VNW28_07350"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXA09783.1"
                     /translation="MTSRPILIAPSILAADFAHLADDLERVLAAGADWLHLDIMDGQF
                     VDNISFGPGLVKTVRGLTSIPFDVHLMIERADHYLPRFVAAGANSITVHVEPEAKHDV
                     AKTLRLIRESGCRAGLTLNPATPFAAVEPHLFRIDLLLIMTVHPGFGGQSFQPAMMGK
                     VRRAAELRDSLGVNYDIEVDGGINPETAQLSLANGANVLVAGTSIFRSDDYAAAIRAL
                     RVPTSEPTIP"
     gene            10881..11327
                     /locus_tag="VNW28_07355"
     CDS             10881..11327
                     /locus_tag="VNW28_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXA09784.1"
                     /translation="MKNDQDNIQLSAVEDHDGILTIRGGGARRDWNGIHYKQGMSAKN
                     VGTTRLSANIATIPPGGVAYAHIHVGFELILYILEGKVRHEFGPGLKNVLENEAGDFI
                     YIKPDVPHEVFNLSKTEPVVAFVARSSADEWDNIIPYDRNQAAGTP"
     gene            complement(11524..13263)
                     /locus_tag="VNW28_07360"
     CDS             complement(11524..13263)
                     /locus_tag="VNW28_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXA09785.1"
                     /translation="MSPALSKSLVDILLASGVASREEASVLAANLNGNSWTADVLNSG
                     KVDEQRFLEAIGQFFSVPVASIDAKKIERATLLVLPSRFVFQHHILPIEIKENAVVLA
                     TYDLFNSTGRQLASQLLSKQAEWVLVPRGQILRAMKTLYGVGAETFDEILKTNRSFEA
                     LQDIETSTDLNADDPEASVVKFVNQIIREAIHERATDIHVEPLENDLRIRYRIDGILH
                     EVAVPPQLRLLQSAIISRLKVMAHMDIAERRLPQDGRINLESADQKIDVRVSTIPTVN
                     GESISLRLLSRDQQQAFGFERLDLSPEHTRIMKSLLAQPNGIILVTGPTGSGKSTSLY
                     CFLSSINSVQRRIITIEEPVEYRLPGVSQIDVKPEIDLTFAKGLRHILRQDPNVVMVG
                     EIRDVETADIAIRAAMTGHLVFSTLHTNDAVGGITRLIDMEVEPFLLASVVKAFLAQR
                     LVRTICPDCVETVEYPREYLEEIGFPIDKGTSFRRGAGCENCRQTGYQGRLAIYEICL
                     VTEPLKRLIMKKADGGELKQCAIAQRMQTLRQDGWRRVAENKTTIEEVVRVTQTDEVM
                     AETTTFAEPVGPR"
     gene            complement(13272..13577)
                     /locus_tag="VNW28_07365"
     CDS             complement(13272..13577)
                     /locus_tag="VNW28_07365"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HXA09786.1"
                     /translation="MADRDEYGLLHQGPTLDRTDFAPRHDWRFPYYFESGVSLISDPA
                     YVGALQSSLRRLGYYCGPIDGIFSDRVRDAIARMQKNYSLPVTGTLTVPVRRALRLP"
     gene            complement(13698..16010)
                     /locus_tag="VNW28_07370"
     CDS             complement(13698..16010)
                     /locus_tag="VNW28_07370"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HXA09787.1"
                     /translation="MLRRLFFGLVPPLFLAVVAFGQVSRPTPFIPLGGAGRPGLPNRP
                     VDMVTMQYPNSDVLDVLHLYENLTGKKLVMDNNVQGKVNIFIAKPVPREEAIKIIEMN
                     LALNGFSLVPAEGDIIEVIGINKNPRAAAVPIFSDPADIPPGAKVVSFLFKLNYADPQ
                     ELQQVLAQYLAGIGGQAPILALPKSSSLLVTQSADVLRKLVKMVEQIDTPPAEVKSEF
                     IKLERADATKVVDMMKEIFDKGNQTGQPGGNIRTIRGVVPPGVPQGSDDEGSAVLSED
                     SLVVGKIKLEADVRTNRIHVITRPINFPFIEKLIAEFDANVEFGKPVTRPLKYIAAAD
                     VLPVLVQALTEPGDQNQQGQGGQGQQQQGRNQRANPTPPNQSNTSSTSGTGGDSTLQV
                     TEELTTQPVDTAPQAVTVGNTKLIADARANSIIVLGNRDVVVKVSKILDEMDVRAPQV
                     ALSTVIGELQLTNDEEFGVDYFVKHSNSVVGVSNNNSRTDVPLPFGAAAAAASPGASA
                     APVNGILDPAGLINFNNILSSVGSGTNVYVSLAAGLSAIVHALDSTGKFKVISRPMVF
                     TSNNKKAIIASGTEIPVPVSTISNTSGSGFINTGLAQQSNIEFKKVALQLEVVPLINS
                     EKEVSLDILQKVDSLAGTTVVSGNSIPNIATRYIRSTVSAPNGATIVLGGLIMDNQQD
                     NRTGIPILGRLPVVGALFRSTTKTKMRNELIILMQPQVTLTKLESYRLRQRTQDRTHF
                     GPELEQDDCPDCPPLADGKQLPPPDLPPQR"
     gene            16101..16538
                     /locus_tag="VNW28_07375"
     CDS             16101..16538
                     /locus_tag="VNW28_07375"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXA09788.1"
                     /translation="MRNIQIRLLMSSLGAVLALSAVAAAAAPEPIRVVLEAQAAAWNR
                     GDIDGFMAGYARSSRTIFVSGNEVTRGWQTVRDRYAKKYDSHEKMGRLTFSGLTITKL
                     CPDVAIVLGNWRLRRRDDEPHGKFTLLFRHLPEGWRIVLDHTS"
     gene            complement(16611..17999)
                     /gene="mpl"
                     /locus_tag="VNW28_07380"
     CDS             complement(16611..17999)
                     /gene="mpl"
                     /locus_tag="VNW28_07380"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HXA09789.1"
                     /translation="MKQSRHFHFLGICGTAMGSVAAALRERGCVVTGSDEKVYPPMSD
                     FLRAKGIRLSEPYRAENLPDEAEVVVIGNAIKRGNPEVEAVLNRKLYYLSLPEVLKEF
                     FLRGRHNLVVTGTHGKTTTTALLTWMLTVGELEPGYMIGGLPRNLGRGALFHESNYFV
                     IEGDEYDSAFFDKRSKFIHYLPELLVINNIEFDHADIFPDLAAVMLSFRRLVNIVPQN
                     GMILLNGDDANCVEVTRDCLAPLVAVGFSENCAERIRDLSSSAKGSRFSLGETTFDSP
                     LFGEFNVRNAAMAAVAARFYGLTPEVIQTALTSFEGIARRQEVRGRARGVTVIDDFGH
                     HPTAIRETLGALRHRYPGQRLWAIFEPRSNTTRRAVFQQQLPEALQLADGVFIAQIAA
                     LEQIPPEERLHPEAVVAAIAAAGRMAFYERDAAAIVERIVPLLQPNDVVTIFSNGGFD
                     GIHGKLLSALRG"
BASE COUNT         3549 a         5572 c         5319 g         3629 t
ORIGIN      
        1 gtaggcaacc acagcaacac ggttctcagc ccgcagtggg gtctgctcat cggcgacacg
       61 accggcgacg gcacggtcaa tgccggcgac atcggccaga ccaaatccca gtcgggccag
      121 ccggtcaccg cctccaactt ccgggaagac gtgacggtcg acggcagcat caacgccggc
      181 gatatcggac tcgttaagtc aaaatcgggc accggtttgc cgtagcctcg gccttcggcg
      241 ctcatccgag gcagagtttg acctttatcc ggtcgcattt ttcgcgctcg ttgccccttg
      301 gcgaggatgt ttctgctgaa gagtcggcca cggatcttct ttaccttggt ggattgccga
      361 tgaaagctca tcggcggttt catcctcgga gcggtggcag ctccaagacc gtcgcaggat
      421 acgcaagcca ctgagattgc cagtactggc ccagcgccga ctaaactaac tctcgcgacg
      481 ccgcggccct cgtcacgttg ccgccgggaa tcatagccgc ctaacgaaca gtcaggatga
      541 cgccacttgc tgaccggcct cagccgcccg cggcgacgcg caggaattcg agtcgatcgt
      601 tttcccgcaa attctgcgtc gcccactcgc ggcgacgcag cgcggcgccg ttcaactcca
      661 ccagcgtcgt ctccggtgca agcgcgagcc gctgaatcag gtcgtggacc gtgccgccat
      721 cttcactctc ggcacgctcg ccgttcacga agattttcac cgcgtgctca gggcctggtc
      781 ccagtccttc caaaccggct caaagccgcg ccgacgcagg acactggcga cctccgccgg
      841 ggaacgctcg tcgctgacct cgaactgacc ggtcgcaagc gcgtcgatcc ccgcatcggg
      901 cggcaggatc cgaccgcgca ccgtctggtg gacggcgtgc cgcccctggc cggtgtagcc
      961 gcccggttca gtatggctcc ccgagctcat gatcgttacg cctaacgacg ccagcgcatc
     1021 gcgcagcggg gcgcgctcgc gcgtgctgag gacgatgccg acctgcggaa agcaaatccg
     1081 aaaagcgcaa accagctgcg ccaactcgcg ttcgcctaac gagaagggcg ggacaaatcc
     1141 tcccgccgcc ggccggaggc gcggcagact aacggtgagg ttggattgcc agcaatgcct
     1201 gagcaaatac tcgagatgag ccgcgagcga gagcgcttcc ggtcgccacg gcgccaaacc
     1261 gaacaacgcc ccgatcccga tccggcggaa accggacgcg gaggcgcgct ccggacagtc
     1321 gagtcgccag ttgaaattgc gcttcgggcc cgccgtgtgc atctctgcat agaccccgcg
     1381 atggtaggtc tcctgataaa cgacgaggcc ctccgctccc gcttcaacca gcggcttgta
     1441 ctccgccgtt tccattggtc ccagctcgag tgagatcgag ggaaaaagtg gcaccaacgc
     1501 gcgaatgcaa tcggcgagat aactgccgct gacaaacttc ggatgctccc ccgccaccag
     1561 cagcacctgc cgaaatccct gccccgccag atgccgcgct tccgcgacca cctgctcgac
     1621 cgtcagggta acgcgcagga tcgggttatc gcgcgagaac ccgcagtagc ggcaattgtt
     1681 gatgcattcg ttgctcaggt agagcggtgc aaacatgcgc atcgtgcgcc cgaaattttg
     1741 cccggtcagc tcgcgcgcct gccgtgccat ccgctcgaac accgcctcgt cgttaggctc
     1801 gaggagcgag gcaaaacgcc gcaacagcgg cgattgcgcc ggcggcaact gatcgaggat
     1861 gggggcaaag gacattccgg caatttcgct tctcccgccg gaatcgcaat tgcgccgcct
     1921 gcgttgtagg attttcgccg tgaccaagcc gaagtttatc cccctgccgc cgcgtgagtt
     1981 cctgagcgat gacgaagtgc tgcggcgggc ggccgatttt catttggaaa tggcgcgtcg
     2041 ccgcacggtg cgcgattttt ccgatcgacc gatctcccgc gcggtgatcg aaaattgcct
     2101 gcgcaccgcg gggagcgcgc cgaacggcgc caacctgcaa ccatggcatt ttgtggcggt
     2161 gagcgatcct ggcttaaagc acgaaatccg ggttgcggcg gaagcggagg agaaggaatt
     2221 ctacgaacac cgtgcgccta acgaatggct ggaggcgctg gccgcgctcg ggaccaacgc
     2281 gcacaagccc tttctcgaga tcgccccctg gctgatcgcg atcttcgcgc agccttatcg
     2341 ccttctgccc aacggacagc ggatgaagca ttactacgcg acggaatcgg tcggcatcgc
     2401 aaccggtctt ctggtcgcgg ccgttcaccg ggccggcctg gtctcgctca cgcacacgcc
     2461 gagcccgatg agcttcctga acaaacttct ccaccggccg gcacacgaaa agccctttct
     2521 cctcctcgtg gtcggtcatc cagcggcggc ggcggtcgtg cccgatatcg agcgcaaacc
     2581 gctcgccgag atcagttcgt ttatcggttg acgggcgcta gaacgcgaag cgcagtcccg
     2641 agcgcaccat gccgaaattg ttgtgatcgc cgttgaccac gttccagctg aaatcgttga
     2701 tgattccgat atggggcgtg acgcggactt cgaagccggc gccaaactgg cccatgccct
     2761 cggtgtcgcc atcatgccgg aagtcgcggg cgaccaggtc ttcgacgcgc gagccattaa
     2821 agatcgcacc gccgccggcg taggcgtaag gcgcaatccg cgtgttcgga attggatagc
     2881 gaaaaatcag attagctgag gcctgaccca ccacgtcgtt cagatcgagg agaaagcctt
     2941 ccacgcccac tccgagataa cgactgaaca tgtagttggc gtcgatgccc ccgccccagg
     3001 cgtgatccgc gtcgaggtag cgatcggtgc gatagggttc gccggtaaag gcataggcgc
     3061 cgaagagatc gatattccat tcccgattcc gataccaatc ctcggtggat tgagaggtcg
     3121 actgctgcgc ttccttgccg gttgtctcca ccggtccggc ctggctgccg gcaacgaaca
     3181 tcatgaccgc gaacaaactg acgataaatt ttttcattcc ctttttctcc ctggttaatt
     3241 gtttcgacag gcggcgccgg aattccctga aacgcgccct tcaacttcca aacggaatca
     3301 aacgtccttc tggatgtcat catccttgca gaggaagaca gcgccgcaac cgccccggtt
     3361 acgcggcttt tttaaccttc tccgaacaaa ggaattgact cggaaaaaat ttctatgtct
     3421 tcagttcggc gccggtgttc gcgtcggtga cgatgaattt ttcgtgatga aaggtcgggt
     3481 cggcccggaa catcaggcgg ccggcataac gccgctccag ttccacgagc aactcttcgt
     3541 cctcttcttt caagcgcttc aagacctcgg gattgaggat cacgcggatg tcgtgaatcg
     3601 agtcctggta ttttcgcatc acggcgttca aggcgcgatg gacttccaca ctggtgctca
     3661 tggtcgtctt gaccacgccg cggccctcgc aataggggca gttttgataa atcgaatcgc
     3721 tcaggctttc ctgcgcgcgc tgccgcgtca tctccatcaa acctaacgag gaaaggggta
     3781 ggacgtgcgt cttcgccttg tcccgcttca gccgctcgcg catccggctg taaaccagct
     3841 gttgatcgcg gcggttcttc atatcgatga aatcgccgat gatcagcccg ccgatgttgc
     3901 gcagacgcag ttggcgcgcg atctcgtcgg ctgcttcgag attggtttgc agaatcgtct
     3961 tctccacatc gcgcccgcct ttgttgcgcc cggtgttcac atccaccgcg acgagcgctt
     4021 cggtttcgtc gatcacgata tagcccccgc atttcagcca gacctggcga tgaaaggcgt
     4081 cgtcgatttg tttctgcacc ccgaaggttt cgaaaatcgg cgcagcgcca tcataatatt
     4141 tgatccggct cttcgcgcgg cgcgagattt cgccgaccat ctgcgccatc cgttcgaccg
     4201 cggcgtggtc atcgcacaac acttcgtcga tctcgtcggt cagaaaatca cgcaccgtcc
     4261 ggtccaccag atcgggctcc tcgaagacgc gcgcgggcgc gggcaattct ttaatcttct
     4321 gctcgatctt cgaccattgc tcgaggagaa aacgcagatc cctgacgaaa tagcgggcgc
     4381 gctgaccttc acccacggtg cggaagatga ctccgatccc gtcgggaatc tcaagttcgc
     4441 gcaggatctt gcgcaagcgc gcacgctctt ttggatcttc gatcttgcgc gagatgccac
     4501 tgcgatcgct aaatggcatg agcaccaggt aacgaccggc gaagctgaga ttggtcgaaa
     4561 cgcgcgggcc cttggtcccg atcggtccct tcgtcacctg cacgatgacc tcggagccaa
     4621 ccggataaat ttcgggaata tccctcgcca ccaggcgctt tttcgaccgt ttctggtcgg
     4681 gccgctcgat ctcttcgatg ccactgtcga gcgcggccgg gatcgcgtcc cagaaatgga
     4741 ggaaggcgtt cttttcgagg ccgatgtcga caaacatcgc cttcaggcca ggctcgatat
     4801 tcttgacccg gcccttgtag atgctgccgg cgatgttgcg cgagctgtcg cgctcgacgc
     4861 tatactcctc gagctggcca tcctcgagca gggcgacacg ccgctcgagt ttctccacgc
     4921 tgataatcaa tttgttgccg gtcttgcgac gcgacagtcc tagaattcgt tttacgtgtt
     4981 cgatcataat gctgcaaatt tttcgaagtc atcgcggggc ttccggtgct gaacttcatg
     5041 ggttaaattc aataggccag ccgcgcgcgt ttcgccggag tcggcgtcgg agcggcgggg
     5101 cgtggacggc tgttaaacag acgctcaaaa aagttgcgcg gacgtggcgg cgccggagtg
     5161 gcaactggca gggcgcgcag ggcgcgtttc ggctgctgcg cattttgtac tttgccccgc
     5221 gcatcggctt ccggttcgac atcgggcgaa gcgttgtcgt tcgccaattg cacaacggcg
     5281 ctttggctgg tatcggcgcc ttcttcgtcc tcgctgccga gattacctcc agtgtagctg
     5341 actgccttga tcaattggag cggattggcc ttatgtgcgg gcgcgagcca ggcgacatgc
     5401 atatcgaact ttcctgcatc ctgcgcgaga gtgcgttcga ggatgcgtgt agcgatcggg
     5461 cctgcgacct cgccaccgtg cccagaggcg ccctgcacca tcactgcgac gacatacttc
     5521 ggatggtcaa atggcgcgaa acacgcaaac cacgccacgt tttcctcgtg gccacggtcg
     5581 gttgcctgcg cagtgccagt tttcccagcc actacgacgt ctttcagacg cgcgcggcca
     5641 cccgttcctc cgtcttcgtt gacaactttc cagagccctt ggcgcacaag atcgatatcc
     5701 tgcggcgaaa cttcctgtcg cagatcggag cggacgcgcg gtgtttgcga aacggcaggt
     5761 ttgccatgct cgtccagcat cggcgagccg tcctggttca aaactttgtc gaccaggcgt
     5821 gggtaataac acgttccacc gttggcggcg gcagcataag ccatcgccaa ttgcagcggc
     5881 gaaacaagcg tgtagccctg gccgatcgaa acattggccg tttgcgcctg cgtccacctt
     5941 tcccggggat ggtgaagttg catccactca gggcccggca tattgccggt ttgttcgcct
     6001 gacagttgca ggcccgactc ttcgccgatt cccaacatct taccaacgac gtcaatcgac
     6061 tgaatgccag ccgcgtttcc gtactggtag aaaaacgagt cgcatgacac cttgattgca
     6121 tcggcaagtc cgatggttcc gtgcgtgtaa tgtttttcgg ctacccagca tcggaaaaag
     6181 tgatcgccgt aactcacacc gccgccgcaa ttgtatttcg cgttcgagag gtctttggga
     6241 ccgcgcaaac cggcgaacga ggtaattaac ttgaacgtcg atcccggcgg cagcgcgctg
     6301 atggcccggt tcacgagtgg gtcgccttcg tctttctgca gcgcctgcca atccttcgct
     6361 ttgatgctcg gaataaatgt attgggatcg aatgacggca ccgacgccat cgcgaggata
     6421 ttcccgttgt ttggatcgac aacgaccgcg ccggctcggc ttaccgcgcg caatgcttcc
     6481 tccgcgatcg cctggatgcg cgcatcgagt gtcagataaa cattcgcgcc ttgtttgggc
     6541 ggctcttccc gtaagagccc gtcgatcgtt cccttggcgt tgcgtcgcat cacccggatg
     6601 ccgggctggc cgcgcaaaaa ttcgtccatc gttttttcga tgttcgattt tccctccgcg
     6661 tcgccctggt aaaaagtgta cttcttcgct tgctcagtgt tcgtgtcgtt aggcgcaccg
     6721 acatagccta acaagtgcga agcgagcgcg ccataaacat aggaacgcac cggcttgatc
     6781 gcgatgtcca ctcccggcaa cccaacatcg tgctcggaaa atttcgccat cgtctcgaaa
     6841 tcgatgtcct tgatgtagga atagggaacc tcggtgtcgt tgcggtaatg cttttgcagc
     6901 tggttcgcgt tgtagtcgcg cgccaggtca agatcctcga ggcggggaac gacgccgtcg
     6961 ttcacgatgc gcacaatgtc cggctccttc agatccttcg gcatcccgct gatcgtcgcg
     7021 cggtagcgcg tctcgggcgg ccggccgtag cgttgccggt aacctttcac catctcgggc
     7081 aggtaaaaat cgacctcgta gctggcgcga ttctgcacca gagcgacgcc gttgcgatcg
     7141 cggatctcgc cgcgaatcga cgggatccgc accgtcgctt ccgagctgct ttggacgcgc
     7201 gccgtccatt cgtagccgcg cgccacctgc acccaccaca gccggaggac gagcgccccg
     7261 agcccaagga gcatgcagag gccgagaaac tggaggcgca gagcgggctt agcgcgacgc
     7321 atattcatcg aagccctcct ctcgcggaac atagggatta ttcgtcaggc gggaaatcca
     7381 gtcgagcgac cagaaaacga gcggggccat caacatcgcg aggaggccgg gcccgccgat
     7441 ttgccaccag acctcgtggt taaaaatgag ggagccgcgg cgaaacgaaa tcataagata
     7501 ttgggcgagg agaataagtg acgtgcagac gccgctgagg acacaatgca cttcccaccg
     7561 cccgcggtta aacaagggct tgagaccgtg catgatggcg gcaagcaccg cgtagaggag
     7621 tatcgaccat ccgaacgaga tttcaacatg cgcgtccagc acctgcaccg tcatcgcgtc
     7681 ccacaaaaaa cccgcgataa aagcaagcgc cagcatgagc ggaaggggca gcgccatcgc
     7741 gccataaaag acgatgaccg gcacgatgta aacgtgcgcg ttgaacatcc actcgagcgg
     7801 cggaatgaaa atttccgcga tttgcgcgac gaagacgagc gcgacaagga cgaggaagaa
     7861 gatcatggcg gaaatgacga agggcgaagg acgaaggacg aaggaatgac aaagcctgaa
     7921 cggccggcac gcgttcgggc ttcgtcattt gtcattcctt cgaccttcgt cattgggaat
     7981 tcggcattct cttctctcat tttctcccga tcaccacgaa gacatcttcc agcttcgcca
     8041 aatccacggc cggcgcgagt ttggcctggc tgtcgagcgc gcggacctca aagctgcgga
     8101 cagtcccgac aaaaagcccg gacggaaaaa ccccgccgac gcccgatgta tagacctttt
     8161 gacccggttt cagttccgct tgtttggaga gataattcaa gtcgagcctt ggggtcagac
     8221 tcccggccac tcgctccccg gtgacaatcc cctgctcgcg cgtgccctcg acactcgctg
     8281 ctacccggca gttttcgtcc gagacgagca gcaccacgga gatattcgcg ctcaccgtcg
     8341 tcgtgcgccc gacgagtccc tcatcggtca gcaccggcat ctccggcgcg agaccgtcct
     8401 cctcgccgcg gttgatcgtg accgtccgcc accaggtcga cgaatcgcgc gagacgactt
     8461 ccgccgggac gagccggaaa agcgagcgct ggcgataatt gagcgcgtgc cgcaggcggt
     8521 tgacctcatg ctcgacgtcg cgcagcgctt cgttcgtcgc tttcagcgag cggttctcca
     8581 cctggagcgc ggcgttgtcg cgttccagtt cctcgagcga tttcagcccg gcgcgcaccg
     8641 aactgatttg ccgctccaaa ccggaaccag tcttgaggaa aggcgaaatg acctggaaga
     8701 aggtggcttg aatcttccgc gtcgtctgcg gtccgatatt cagaagcgcg agaagggccg
     8761 cggcgaaaag gacgatcgcg acgatactgc tgcggctcat cgcagctcag cattcaaatc
     8821 tctgatttcg gattcgatca tctccaatca gcaaccagca atcgattcac tgccgccact
     8881 ggcgatcggc cgtcgcgacc tggcggagga acttgatctc gttcaaacat ttgcccgtcc
     8941 cctctgcgac cgcgctcaac ggatcttccg cgacgtgcac cggcagcccg gtctcctcgc
     9001 tcaacaagcg atcgaacccg cgcaggagcg cgcccccacc cgcgaggacc aacccgcgat
     9061 cgaccagatc ggccgacaat tccggcggac aacgctcgag cgtgatccgc accgaatcga
     9121 caatcgtcga gattggttcg agcaacgcct cgcgcacttc ctgcgaagtg atcgtcaggg
     9181 tctttggcag cccggcgacc aggtcccggc cttttacttc cacgctcgtc tctttgtcgc
     9241 tgggataagc ggaaccgatt ttgattttga tctcctccgc cgtgcgctcg ccgatcatca
     9301 ggttgtaggc gcgcttcatg tactgcacaa tcgcttcatc cagctcgtcg cccgccactc
     9361 gcacgctccg gctgaagacg atgcccgaga gtgaaatcag cgcgacttcc gtcgtcccgc
     9421 cgccgatgtc gatgatcata ttgccggccg gatcctgcac cggcagtccg acgccgatcg
     9481 ccgccgccat cggttcttcg atcaggtaaa cttcccgcgc cccggcgtgc gtggccgatt
     9541 cccgcaccgc gcgtttttcc acctcggtaa tcccggacgg cacggcgatc acaacccgcg
     9601 ggcgggcgcg tacccggctg ttgtgcacct tggtgatgaa atgccgcagc atcgcctcgg
     9661 ttacctcaaa atcggcaatg acgccgtctt tcaatggccg aacggccaca atattggccg
     9721 gcgtgcgccc gagcatgcgc ttcgcttcgt cgccgacggc aagcactttc atcgtgcccg
     9781 cctgcaccgc cacgaccgac ggttcgcgca ggacgatgcc ctgatccttg acgtaaacga
     9841 gtgtgttggc ggtgccgaga tcgatcccga tatcgctgga gaaccaaccg aaaagtctat
     9901 taagcatgag agaagccttg gaatggagtc cgccggccga cccattacaa gcgaaagctg
     9961 tgccgcacgt ccggaccggc gaaaaacccg cagtatcttg gaacggcgca gagtgccgtc
    10021 aagggaaagc tggccggaca gctggcggac aacgttcaac gtccaatctc caacgctcaa
    10081 cgtttaacgt ccgaatcgac ctggtcccgc tgtcctaaat tgaaagttga acgttgaacg
    10141 ttggacgttg aacgtttctc ctctccttcg atccacctcc gatgacctcg cgtcccatcc
    10201 tcatcgctcc ctcaattctc gcggccgatt tcgcgcacct cgccgacgac ctggaacgcg
    10261 tcctcgccgc cggcgccgac tggctgcatc tcgacatcat ggatggtcaa ttcgtcgaca
    10321 acatttcctt cggacccggg ctcgtaaaga cggttcgtgg cctaacgagc atcccgttcg
    10381 atgtccattt gatgatcgag cgagcggatc attacctccc ccgtttcgtc gccgccggcg
    10441 cgaactcgat tactgtccac gtcgagccgg aagcgaagca cgatgtcgca aaaacgctcc
    10501 gattgattcg ggaaagcggc tgccgtgccg gcctgacgtt gaatccagcg acgccttttg
    10561 ccgccgtcga gccgcacctc ttccgcatcg atcttctcct catcatgacc gtccatcccg
    10621 gcttcggcgg ccaatctttc caacccgcga tgatgggaaa agtgcggcgt gcggccgaac
    10681 ttcgcgattc gttaggcgtg aactacgaca tcgaggtcga tggcgggatc aatccggaga
    10741 cggcgcagct ttcgctcgcc aacggcgcca acgttctggt cgccggcact tccattttcc
    10801 ggtcggacga ttacgcggcg gcgattcgcg ccttgcgcgt tcccacctct gaaccaacga
    10861 ttccctagga aagatcgatt gtgaaaaacg accaagacaa catccagctc agcgcggtcg
    10921 aggatcacga cggcatcctg acgattcgcg gcggcggggc acgaagagat tggaatggca
    10981 ttcattacaa acagggcatg tcggcaaaaa acgtcggcac gaccaggctt tccgccaata
    11041 ttgcgacgat tccgccgggc ggtgtggcct acgcccatat ccacgtcggc ttcgagctca
    11101 ttctttacat tctcgaagga aaagtgcgcc atgaattcgg gcccggcctc aagaacgtgc
    11161 tcgaaaacga agccggcgac ttcatttaca taaaacccga cgtgccccat gaggttttca
    11221 acctgagcaa gaccgaaccg gtggtcgcgt ttgtcgcccg ttcctcggcc gatgaatggg
    11281 ataatattat cccgtacgat cgcaaccagg ctgccggaac gccatagtca aggcgcgcct
    11341 tcctgtcagt gaccgtctct gcccggcgtt gcctaacaag aggaacagga ccaggggcat
    11401 tttgatcgct cgattcaaac tgtcaggctt gcctgagtgc aagccttttt ggaggcggcg
    11461 gtacgccttc caagccgccc ggttggactg cgccccgagc cggccaagtc gagttcggcg
    11521 gtgctaccgc ggcccaaccg gctcggcgaa ggtggtggtc tcggccatca cttcgtcggt
    11581 ctgtgtcacc ctcacgactt cttcgatcgt ggtcttgttt tctgcgacac ggcgccagcc
    11641 gtcctgccgg agggtctgca tgcgctgggc gatggcgcat tgttttagtt cgcccccgtc
    11701 ggcttttttc atgatgaggc gcttgagcgg ttcggtcacg aggcaaatct cgtagatggc
    11761 gagccggccc tggtaaccag tctggcgaca gttttcgcaa ccggccccgc gcctgaagga
    11821 cgtgccttta tcaatcggaa agccgatttc ttcgaggtat tctctcggat attcgaccgt
    11881 ctcgacgcaa tcgggacaga tggtgcgaac aaggcgctgg gcgaggaagg ccttcacgac
    11941 cgaggcgagc aggaacggct cgacctccat gtcgatgaga cgcgtgatgc cgccgacggc
    12001 gtcgttggtg tgcagggtgc tgaagacaag gtgaccggtc atggcggcac gaatcgcgat
    12061 gtcggcggtc tcgacgtcgc gaatttctcc gaccattacg acatttgggt cctggcgcag
    12121 gatgtgacgc aggcccttcg cgaaggtgag gtcgatctcg ggttttacgt cgatctgaga
    12181 aacgccgggg agtcgatact cgaccggctc ctcaatcgtg ataatgcggc gctggacgga
    12241 gttgatgctg ctgaggaaac aatagagcga agtcgatttg ccggaaccgg tcgggccggt
    12301 aacgagaatg atgccgttag gctgggcgag gagcgacttc atgatccggg tgtgctcggg
    12361 gctaaggtcg agccgttcaa agccgaacgc ctgctgctga tcgcggctga gcaaacgcag
    12421 gctgatcgat tcaccgttca ccgtcgggat ggtggaaacg cggacgtcga ttttctggtc
    12481 ggcgctctcg agattgatcc ggccgtcctg cgggaggcgg cgctcggcga tgtccatgtg
    12541 cgccatcact ttcaagcgcg agatgatggc cgattgcaga agacgcaatt gcggcggaac
    12601 cgcgacttcg tgcaggatgc cgtcgatccg gtaacgaatg cgcagatcgt tctcgagcgg
    12661 ctcgacgtga atgtcggtcg cgcgttcgtg gatcgcttcg cgaatgattt ggttgacgaa
    12721 cttcacgacc gaggcctcgg ggtcgtcggc gttgaggtcg gtgctcgtct cgatgtcctg
    12781 gagcgcttcg aaggagcggt tggttttgag gatttcgtcg aacgtttccg cgcccacgcc
    12841 gtagagcgtt ttcatcgcgc ggaggatttg cccgcgcggc acaagcaccc actcggcctg
    12901 tttgctcagc agttgggagg caagctgccg gccggtcgag ttaaacaaat cgtaggtcgc
    12961 gagcacgacc gcgttctcct tgatctcgat cggcagaatg tgatgctgaa aaacgaagcg
    13021 gctcggcagg acgagcaagg tcgcacgctc gattttcttc gcgtcgatcg aggccaccgg
    13081 cacgctgaag aattggccga ttgcctcgag gaagcgttgc tcatcgacct ttcccgagtt
    13141 gaggacatcg gcggtccacg agttgccgtt gagattcgcg gccaggacgc tcgcctcttc
    13201 gcgggaagct actccgctgg cgagcaggat atcgacgagt gatttactga gggccgggct
    13261 catgccgcaa tctaaggcaa acgcaacgct ctgcgcaccg ggacggtgag agttccggtc
    13321 acgggaaggg aatagttttt ttgcatccgc gcgatcgcgt cgcggactct atcagagaaa
    13381 attccatcga tcgggccgca atagtagccg aggcgacgca aagaagactg gagggcgcca
    13441 acgtaagccg gatcggagat caggcttact cctgactcga aatagtaggg aaagcgccaa
    13501 tcatggcggg gcgcgaagtc ggttcgatcc agcgtcgggc cctggtgaag cagcccatac
    13561 tcatctcgat cggccaacgg ctcactagaa ttaaaaagtc tgtcaaagaa aattgactcg
    13621 tccgcaaaac tagcccgcgg tatacaaaag gacattgcca aaacaatgag agcaagaact
    13681 cggttcatga tgggacgcta tctttgtggc ggaagatcgg gtggtggcaa ttgttttccg
    13741 tcggccagcg gcgggcaatc gggacaatcg tcctgttcca gttcaggccc gaaatgggtt
    13801 cggtcctgcg ttctctgacg caggcgatac gactccaatt tcgtcagggt tacctgcggt
    13861 tgcatcaaaa taatcagttc atttcgcatt ttggttttgg tcgtgctgcg gaaaagagca
    13921 cccaccaccg ggagacgacc cagaatgggt attccagtcc ggttgtcttg ttggttgtcc
    13981 atgatcagtc cgcccagaac aatggtggcg ccgttcggcg ccgagacggt cgagcggata
    14041 tagcgggtcg caatgttcgg gatggaattg ccagagacca cggtggtacc cgccaggctg
    14101 tcaaccttct gcaaaatatc gagcgagacc tctttttcag agttgatcag cggcactact
    14161 tcgagttgga gcgccacttt cttgaactca atatttgatt gctgcgcgag cccggtgttg
    14221 atgaagccgc ttccgctcgt atttgagatc gtactcaccg gaacggggat ctccgttccc
    14281 gaggcgataa tcgccttctt gttgttgctc gtaaagacca tcggccgcga aatcaccttg
    14341 aatttccccg tcgaatcaag cgcatgcacg atggccgaga gaccagcggc gagagagaca
    14401 taaacgttgg tgccggatcc cacgcttgac agaatattat tgaagttgat caggccagcg
    14461 ggatcaagga ttccattaac aggggcggcg gaagcacccg gagaggctgc agcagcggct
    14521 gcgccaaatg gaagaggcac gtcggtcctg ctattgttat tcgaaacgcc caccaccgag
    14581 ttactgtgct tcacaaaata atcgacgccg aattcctcgt cgttggtcag ttgcaactcg
    14641 ccaatcacgg tgctcagggc gacctggggc gccctcacat ccatttcgtc gaggatcttg
    14701 gaaaccttca ccaccacgtc gcgattgccg agaacaatga tcgagttggc gcgcgcgtcg
    14761 gcgataagct tggtgttgcc gaccgtgacc gcctgcggcg cggtgtcgac cggttgcgtg
    14821 gttaactctt cggtcacctg cagcgtgctg tcgcctccgg tgccggaggt gctgctggtg
    14881 ttcgattgat tggggggcgt cggattggcg cgctggttcc gcccctgctg ctgctgcccc
    14941 tggcctccct gcccctgctg gttctgatcg cccggctcgg tcaacgcctg gaccaacacc
    15001 ggcagaacat cggccgccgc gatgtatttc agcggacgcg tcaccggctt gccgaactcc
    15061 acgttggcgt cgaattccgc aatcagcttc tcgataaacg ggaaattgat tggccgggtg
    15121 atgacgtgga tgcggttggt gcgcacgtcg gcttcgagct tgatcttgcc gacgacgagc
    15181 gaatcctccg aaagcacggc cgacccttca tcatcgctgc cctgcggtac cccaggcggc
    15241 acgacgccgc ggatggtacg gatatttccg ccgggctgtc ccgtctgatt ccctttgtcg
    15301 aaaatctcct tcatcatgtc gaccactttg gtcgcgtcgg cccgctccag tttgatgaat
    15361 tcgcttttca cttcagccgg gggggtgtcg atttgttcga ccatcttcac cagtttccga
    15421 aggacatccg cgctttgcgt tacgagcagg ctcgaagact tgggcagcgc caggatcggc
    15481 gcctgcccgc cgatgcccgc cagatattgc gcgagcacct gctggagttc ctgcgggtcg
    15541 gcgtaattca acttgaagag gaagctgacc accttcgcgc cgggcgggat atccgcggga
    15601 tcggagaaaa tcggcacggc cgccgcacgg ggattcttat tgattccgat gacctcgata
    15661 atatcgcctt ccgcgggcac gagcgaaaat ccgttcaggg ccaggttcat ctcgatgatc
    15721 ttgatcgcct cttcgcgcgg caccggtttg gcgatgaaga tgttcacctt gccctggacg
    15781 ttgttgtcca ttaccagctt cttacccgtg agattttcgt agaggtgcag gacatcgagc
    15841 acgtcgctgt tcgggtattg cattgtcacc atatcgacgg gccggttcgg caagccgggc
    15901 cggcccgcgc ccccgagcgg gataaatggc gttggcctgg acacctgccc aaaagcgacc
    15961 acggcaagaa aaagcggcgg gacgagtcca aagaagaggc gacgcagcac gcgcttatgt
    16021 aattgttcgc cgccccgagg tcaaccagtt ctccggggag aagattcgac tggttgtgga
    16081 aattgcggaa tgctcagctt atgcggaaca tccaaatacg gctgctcatg agttcattag
    16141 gcgccgtcct cgccctgtcc gcggtcgcgg cggcggccgc gccggagccc attcgcgttg
    16201 tgctcgaagc ccaggcggcg gcctggaatc gcggcgatat cgacggcttc atggcgggct
    16261 acgcgcgctc gtcccggacg atcttcgttt ccggcaacga agtgacccgc ggctggcaga
    16321 cggtgcgcga tcgttatgcc aaaaaatatg acagccacga gaagatgggc aggctgacct
    16381 tttccggcct aacgattacg aagctttgtc cggatgtcgc gatcgtcctc ggcaattggc
    16441 gcctgcggcg cagggacgac gagccgcacg ggaaattcac gctcctcttc cgccacctgc
    16501 ccgagggctg gcggattgtg ctggatcaca cctcgtgagc ccggagggac gcgcttccgc
    16561 gcgcccagga catgcgcaag catgtccctc cgaaggcggc gagacgccgc tcaaccgcgc
    16621 agcgcgctca gcaacttccc gtgaattccg tcgaagccgc cgttgctgaa gatcgtgacc
    16681 acgtcgttag gctggaggag cggcacgatc cgctcgacga tcgcggccgc gtcacgctcg
    16741 taaaatgcca tccggcccgc ggccgcgatc gcggcgacga ccgcctccgg gtgaagccgt
    16801 tcctccggcg gaatctgctc gagggccgcg atctgcgcga tgaaaacgcc gtcggccagt
    16861 tgcagagcct caggcaattg ctgctgaaag accgcgcgcc gcgtcgtatt cgagcgcggc
    16921 tcgaaaatcg cccaaagccg ctgccccgga taacgatggc gcagcgcgcc taacgtctca
    16981 cgaatagcgg tcggatgatg cccgaagtcg tcgattaccg tcaccccgcg cgcccggccg
    17041 cgcacttcct ggcggcgcgc gatcccttcg aagctggtca gggcggtctg gatcacttcc
    17101 ggagtgagac cgtagaagcg ggccgcgacc gccgccatgg cggcattgcg gacgttgaac
    17161 tcgccgaaga ggggcgagtc gaaagtcgtt tcgcctaacg aaaagcggga gcccttggcc
    17221 gaggagctga gatcgcgaat ccgttccgcg cagttctccg agaagcccac tgccacgagc
    17281 ggcgcgaggc aatcgcgcgt cacctcgacg cagttcgcgt cgtccccgtt gagcaaaatc
    17341 attccgttct gtggcacgat gttcaccagc cggcggaagc tcagcatgac cgctgcgaga
    17401 tcgggaaaga tatccgcgtg atcgaactcg atgttgttga tcacgagcag ctccgggagg
    17461 tagtgaatga acttggaacg cttgtcgaaa aacgccgagt cgtactcgtc gccttcgatc
    17521 acgaaataat tcgactcgtg gaaaagcgcg ccccgcccca ggttgcgcgg cagaccaccg
    17581 atcatatagc cgggctccaa ttccccgacc gtcagcatcc aggtgagcag cgcggtcgtc
    17641 gtcgttttcc cgtgcgtgcc ggtgacgaca agattgtgcc ggccgcggag gaagaattcc
    17701 ttcagcactt cggggagcga cagataataa agcttccggt tcaggaccgc ttccacctcc
    17761 gggttgccgc gcttgatcgc gttgccgatc accaccacct cggcctcgtc aggcaaattc
    17821 tccgcgcggt acggctcgct cagtcggatc cccttggcgc ggagaaagtc ggacatcggg
    17881 ggatagacct tttcgtccga cccggtcacg acgcagccgc gctcgcgcaa ggcggccgcg
    17941 accgaaccca tcgccgtccc gcaaattccg aggaaatgga aatggcggga ctgcttcacg
    18001 cccgcacctc taagctgacc ggcaaacttt tgcgagcgac agaccatgca gggcgcctca
    18061 gttcacggc
//
LOCUS       DATMNS010000212         3231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2805667, whole genome shotgun sequence.
ACCESSION   DATMNS010000212 DATMNS010000000
VERSION     DATMNS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2805667"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VNW28_07385"
     CDS             <1..499
                     /locus_tag="VNW28_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXA09790.1"
                     /translation="LSSVLLVFQLGQPRIFMAMARDGLLPQYFARIHPRFRTPHVTTI
                     WTGLAVGGVAMLTDIGSLADLTNIGTLFAFALVCSGVIVLRRKDPTRPRPFRTPFVPF
                     FPILGVLMCIALMLSLPIETWLRFFGWLIIGLAIYFLYSVRHSRLRHGIDTGETENEF
                     PPIVP"
     gene            complement(537..2471)
                     /locus_tag="VNW28_07390"
     CDS             complement(537..2471)
                     /locus_tag="VNW28_07390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXA09791.1"
                     /translation="MKYLLVLILAGLLASPVFGQGPSPAAPASPVAKADSAIPPKIPL
                     RDFFKNPVSRGYDLSPDGATLSFLQPWDSRMNVFIRPTAGGEAKRLTNEKDRDIRNYA
                     WKGNKFIVYAMDDKGDENFHLKRVDLKSGEVKDLTPFPKVRSDIIDDLADASETDMLI
                     TLNKRNPEIFDAFRLNVATGEMKMVAQNPGKVDRWITDHAGVIRAATQTDGVNTSLLT
                     RLDEKAPFKEVLKTNFRESVSPQFYTFDNKSIYATSNIGRDKAAVVTLDPSNGKELEM
                     IYQNPEVDVDALAYSKKRKVVIFAAFDTWKTERKFFDPQTEAIYKTLQEKLPGYEVEV
                     VANDKAEDKFIVVASNDRSPGSRNLFDVKTGMLTKLVDVAPWLKESELAPMKPIEYQS
                     RDGLTIHGYLTLPLGRSARNLPVVINPHGGPWARDTWGFNPEVQFLANRGYAVLQMNF
                     RGSTGYGRKFWEASFKQWGQSMQNDITDGVQWLVKQGVADPKRVAIYGGSYGGYATLA
                     GVTFTPDLYAAAVDYVGVANMFTFLKTIPPYWKPFLDMFHEMVGDPEKDKAMMEAVSP
                     VMHADKIKTPLFVAQGAHDPRVNKDESDQMVAALKKRGVEVEYMVKDNEGHGFHNEEN
                     RFDFYGAMEKFLAKYLKPAA"
     gene            2496..>3231
                     /locus_tag="VNW28_07395"
     CDS             2496..>3231
                     /locus_tag="VNW28_07395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HXA09792.1"
                     /translation="MLPLDSSVDATLAQVRGVAFHFVGQRIRLAGVTEAIQTFNLLGT
                     PLLATSYERLIAQTHALALEGATCAIDLTNTHIVTLRRQEAHFRELTSRFDYFVPDGM
                     PLIWCLNARGAGLRDRVYGPTFMRRCLEASPAPWKHYLLGGSVECVARLRTRFSPPAQ
                     IVGARDGYFAPDEETEILDEINRLEPDFIWIGLGTPKQQDWIYRHKAAIRRGVLFAVG
                     FAFDVNAGMKPDAPAWMQRLGLTWLFR"
BASE COUNT          569 a          976 c          937 g          749 t
ORIGIN      
        1 cctttcctcg gtgcttctcg tcttccagct cgggcagccg cgaatcttca tggcgatggc
       61 gcgggatggt ttgttgccac aatattttgc ccgcatccac ccgcgcttcc gcaccccgca
      121 cgtgaccacc atctggaccg gtttggcggt cggtggcgtg gcgatgctga cggatattgg
      181 ttcgctggcc gacctgacca atataggaac acttttcgcc ttcgccctcg tttgttctgg
      241 cgtaattgtc ttgcgcagga aagatcccac ccgaccgcgt cctttccgca ctcctttcgt
      301 gccgttcttt ccgattctcg gagtcctgat gtgcatcgct ctcatgctca gcctcccgat
      361 cgaaacctgg ttgcggttct tcggctggtt gatcatcggc ctcgccattt atttcctcta
      421 cagcgttcgg cacagccggc tccgccacgg gatcgacacc ggggaaaccg agaacgaatt
      481 cccgccgatc gtgccctagg aagacgcgcg aaaaaatccg cgttcgttag gcgaagctag
      541 gctgccggct tcagatattt cgcgagaaac ttctccatcg cgccgtagaa atcgaaacgg
      601 ttttcttcgt tgtgaaagcc gtggccttcg ttgtccttca ccatgtattc gacctccact
      661 ccgcgctttt tcagcgccgc caccatctgg tcggactcgt ccttgttcac ccgcggatca
      721 tgcgcgccct gcgcgacaaa gagcggcgtc ttgatcttgt ccgcatgcat cacgggcgag
      781 accgcttcca tcattgcctt gtctttttcc gggtcgccca ccatctcgtg gaacatgtcg
      841 agaaacggtt tccagtaggg cgggatcgtc ttcaggaagg taaacatgtt ggccacgccg
      901 acgtaatcga cagccgccgc gtagagatca ggcgtaaagg tgacgccggc gagcgtcgca
      961 tagccgccgt agctgccgcc gtaaatcgcg acccgcttcg gatcggcgac cccttgttta
     1021 accagccatt gcacaccgtc ggtgatgtcg ttctgcatcg attgtcccca ctgcttgaag
     1081 gacgcttccc agaacttgcg gccgtagccg gtcgagccac ggaagttcat ttgcagcacg
     1141 gcatagccgc ggttggcgag gaactgcacc tcgggattga agccccaagt gtcgcgcgcc
     1201 catggaccgc cgtgtgggtt gatcaccacc ggcagattcc tggccgatcg gccgagcggc
     1261 agcgtcaggt agccgtgaat cgttaggccg tcacggctct ggtattcgat cggcttcatc
     1321 ggcgcgagct cgctctcctt tagccaaggg gccacatcca ccagcttcgt aagcatgccg
     1381 gttttcacgt cgaacaaatt gcgcgacccc ggcgagcgat cgttgctcgc cacaacgatg
     1441 aatttgtcct cggccttgtc gttggcgaca acttccactt cgtagccggg caatttttcc
     1501 tgcagggttt tgtagatcgc ctccgtttgc ggatcgaaaa acttgcgttc cgttttccag
     1561 gtatcgaagg cggcgaaaat taccaccttt cgctttttcg aataggccag cgcatccacg
     1621 tccacctcgg gattttggta aatcatttcc agctccttgc cgttgcttgg atcgagcgtc
     1681 acgaccgccg ctttgtctcg gccgatgttg gaagtcgcgt agatgctttt gttatcgaag
     1741 gtgtaaaact gcggcgatac cgactcgcga aaattggtct tcaacacctc cttgaatggc
     1801 gccttctcat ccagccgggt gagcaggctg gtgttcacgc catcggtctg ggtggccgcg
     1861 cggatcacgc ccgcgtggtc ggtgatccag cgatccactt tgccgggatt ctgggccacc
     1921 atcttcatct cgccggtcgc gacgttgagc cgaaatgcgt cgaagatctc cgggttgcgt
     1981 ttgttcaggg tgattagcat gtcggtctcg gaggcgtccg cgagatcgtc gatgatgtcg
     2041 ctccgcacct tgggaaacgg cgtcaggtcc ttcacctcgc cgctcttcaa gtcgacgcgc
     2101 ttgagatgga aattctcatc gcccttgtcg tccatcgcgt agacgatgaa tttatttccc
     2161 ttccaggcgt agttgcgaat gtcgcggtct ttctcattcg ttaggcgttt cgcctcgccg
     2221 cccgccgtcg ggcgaatgaa gacgttcatc cgcgagtccc acggctgaag aaaggagagc
     2281 gtggcaccat ccggcgagag atcgtagccg cggctgaccg ggttcttgaa aaaatcccgg
     2341 agcggaattt tcggcggaat cgcggaatcg gccttggcga ccggtgaagc cggagccgcg
     2401 ggtgacggtc cctggccgaa gacgggactg gcgaggaggc cggcaagaat gagaacaagg
     2461 agatatttca tggcgttgtg agtgagatgc agcgcgtgct gcctttggat tcaagtgtcg
     2521 atgccacgct cgcgcaagtg cggggggtcg cttttcattt cgtcggccag cggatacggt
     2581 tggcaggcgt gacggaagcg atccaaactt tcaatctgct cggcacgccg ctgctcgcga
     2641 ccagttacga aaggttaatt gcccagactc acgccctcgc gctcgagggc gcgacctgtg
     2701 cgatcgacct gacgaataca cacattgtca ctctgcggcg gcaggaggcc cacttccgcg
     2761 agctgacgag ccggttcgat tatttcgtgc ccgacggcat gcccctcatc tggtgcctga
     2821 acgcgcgcgg cgccggcctg cgcgaccggg tttatggccc gactttcatg cgccgttgcc
     2881 tcgaggccag tcctgctccc tggaagcatt atttgttagg cggctccgtc gagtgcgtcg
     2941 cccggctgcg cacgagattt tcgccgcctg cccaaattgt cggcgctcgg gatggctatt
     3001 ttgcgcctga cgaggaaacg gaaatcctcg acgagatcaa tcggctggag cccgatttta
     3061 tttggatcgg gctcggcaca ccgaaacaac aggattggat ctaccggcac aaggccgcga
     3121 tccgacgcgg cgttttgttt gcggtgggat ttgcttttga cgtcaacgcc gggatgaaac
     3181 ccgatgcgcc cgcctggatg cagcgcctcg gactaacctg gcttttccgc g
//
LOCUS       DATMNS010000213         7300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4089268, whole genome shotgun sequence.
ACCESSION   DATMNS010000213 DATMNS010000000
VERSION     DATMNS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7300
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4089268"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VNW28_07400"
     CDS             <1..695
                     /locus_tag="VNW28_07400"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXA09793.1"
                     /translation="AEEIPLEILFEDDDLLVLNKPTGLVVHPGAGNQTHTLVNALLHH
                     CASLSGIGGKQRPGIVHRLDKETSGCLVVAKNDAAHQHLARQFAEREVKKIYLALVSG
                     VPKKPRGTIEAPIGRHPVQRKKMAVHPTRGRIAKTDYRVLQSGGGVSLVECAIHSGRT
                     HQIRVHLHYIGHPVVGDSLYGKKVAGPRQMLHAWKLGFTHPRTGERHFLEAPIPEDFR
                     RWRDAAFTPATR"
     gene            complement(676..2001)
                     /locus_tag="VNW28_07405"
     CDS             complement(676..2001)
                     /locus_tag="VNW28_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09794.1"
                     /translation="MPIVRQAWFWLALLAEILLLAALHRFDDWHYATMPVKFVETAVL
                     CGIGFFAAASAFAKLPLGRMGAVIFWSVAILLRTVALPLEPGDDIWRYQWEGRVQNAG
                     FNPYVFSPNDEQLAPVRAQFKDWNQINHRDFSAIYPPGTELIFAGLSRFAADPLFYKL
                     LFAAADLGAIAVLLRLIGGPARYADAAWYAWNPLVVYSFAGAAHFDSLMILAMLAGIL
                     CFVRSRVATDAAGQWRWAVLGAGALGAAISIKLVPVLILPVCVFALGRRAWALALSLG
                     IPALLSLPFGFPRVPIWQSLGRFIYIARLNDMFWWLIEESFWPNPHQKNYHYNVVIIV
                     AVVLVSLLFMRNWRRGLLWVMGTALILSPVLHPWYCTWILPLAAWRRADAWQVLSITV
                     FAYYLFWDERLFLLPWHSEPWLRSFILIPPIVAALLALRPKSPPNESPA"
     gene            complement(1988..4519)
                     /locus_tag="VNW28_07410"
     CDS             complement(1988..4519)
                     /locus_tag="VNW28_07410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA09795.1"
                     /translation="MSQTLPVRVRSLAKFFFEGDRKFFVKGATYGPFRPDAAGDYLGS
                     EEQARRDLTQMRALGINVVRTYHLPPRWFLDACSEAGVRVLITLPWAKHVEFLVRAKT
                     RREIVRAVRAAVALQAGHPAIFGYLVGNEIPTTMVRWLGVRRVTEFLETLIRVGREAD
                     PSVLFSYASYPPTEYLLPQNVDFYCFNVYLHDQRDFEKYLLRLQNLAEEKPLMLGEFG
                     MDTIRHSEEEQAEMLGWHVDSVVRCGLAGTIFFAWTDEWFTGETEITDWSFGLVRRDR
                     TPKKSFFSLQKKLGQDDASLPHRDLPQTPFVSVIVCSYNGAKTLAECLESLGKINYPT
                     YEAILVDDGSTDNTPEIAARYPKVRYIRQENHGLSYARNAGAAAAQGQVLAYTDSDCM
                     ADPDWLYYLIGTLVSGDYAGVGGPNVSPPAENWVQACVAAAPGGPSHVLLTDTVAEHI
                     PGCNMAFYRWAFDTVGGFDIEYRKAGDDVDFCWRLQQEGHVIAFSPTAIVWHHRRFTL
                     GAFRKQQAGYGEAESMLRFKHLIFFGPTGTAKWRGQIYGSPRFSWFINRPIIYHGIFG
                     EGFFQSIYPSPQSEIANYLSSIEWFVLTLFLFGLGIFLPVLRIVPYLMLGGTLCVALS
                     YMLRARIEPRFDTVAARLLVMFLAFAQPLVRGWNRYFTWLEFKRTPRGVIGTHEKMPS
                     GTAGRGSLRRRNYWSEAGVERNALLKSIFQLLEEEGWSYSADTGWKEWDIQIYGNFFW
                     SVVLQTVTEYHGGPKCLTRVRLRYRFVTTTVILNLLVLAMILYRDLNSAGVDLRILVP
                     YAIFLLFLGTRARRLKRRVAEIVDVAAYRLGLQRITRKRTPDADR"
     gene            complement(4578..5864)
                     /gene="hemL"
                     /locus_tag="VNW28_07415"
     CDS             complement(4578..5864)
                     /gene="hemL"
                     /locus_tag="VNW28_07415"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HXA09796.1"
                     /translation="MPAQSSELFARACRLIPGGVNSPVRAFRGLGREPFFVERAAGAN
                     IWDVDGKMYIDYVGTWGPAILGHAPGAVIEAIRAAAGRGVSFGIPNPLEVEMAELICR
                     WVPSIEKVRMVNSGTEATMSCVRLARGFTGRDKIIKFEGCYHGHVDSLLVRAGSGALT
                     HGQPDSAGVPEAFAGLTIALPFNDIEAVRETLRANPNEIAAIILEPIPANAGLYFPQP
                     GFLAALREECTQHGALLIFDEVMTGFRVARGGAQEVYGVRPDLTALGKVIGGGLPVGA
                     FGGRAEIMDRLSPLGPVYQAGTLSGNPLAMAAGLAQLRELERTDGWKALETLGAQLEE
                     AMRDAIGETKTPAVFHRIGSMFCLFFADEPVNDLAGARRSNLEKFARFFTHCLEAGIY
                     FAPSQFETGFISTAHRPDQIEATARVVREALARLSR"
     gene            6620..6691
                     /locus_tag="VNW28_07420"
     tRNA            6620..6691
                     /locus_tag="VNW28_07420"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6652..6654,aa:Cys,seq:gca)
     gene            complement(6796..7119)
                     /locus_tag="VNW28_07425"
     CDS             complement(6796..7119)
                     /locus_tag="VNW28_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09797.1"
                     /translation="MIERPDLARELAKPDAINAITPMQSAAITMRERAERHVARMAGV
                     TDKVLPHLESMQPAEILDSARNLERFDYVARRNCGLENQPVPGGLFSINVLANHSLVQ
                     FVGGA"
     gene            complement(7112..>7300)
                     /locus_tag="VNW28_07430"
     CDS             complement(7112..>7300)
                     /locus_tag="VNW28_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09798.1"
                     /translation="ADYVHSNMSRIPASTWAEIRTAYASGIGLRELARNMGIPAGDCA
                     RSRQARTLDAANRNREID"
BASE COUNT         1564 a         2222 c         2228 g         1286 t
ORIGIN      
        1 aggcggagga aatcccgctc gagatccttt tcgaagacga tgatctgctc gtgctgaaca
       61 agccaaccgg tctggtcgtt catcccggcg ccggcaacca aacgcacacg ctcgtcaacg
      121 cgttgcttca tcattgcgcc agcctttccg ggatcggcgg aaagcaacgg cccgggattg
      181 tgcatcgcct cgataaggaa acgagcggct gccttgtcgt ggccaagaat gacgccgcgc
      241 atcagcatct cgcccgccaa ttcgccgagc gcgaggtgaa gaaaatttac ctcgcactcg
      301 tctctggcgt cccgaagaaa ccgcgcggca cgatcgaagc gccgattggc cggcacccgg
      361 tgcaacgcaa gaaaatggcg gtccatccga cgcgcgggcg aatcgcaaag acggattacc
      421 gcgtgcttca atctggcggc ggggtcagcc tggtggaatg cgcgatccac agtgggcgca
      481 cccaccagat tcgcgtccac ctgcactata ttggccatcc cgtggtcggt gattctctct
      541 atgggaaaaa agtcgctggc ccgcggcaga tgttgcacgc ctggaaactc ggtttcaccc
      601 acccgcggac cggcgagcgc cattttctgg aagcgccgat cccggaagat tttcgacggt
      661 ggcgggatgc ggcctttacg ccggcgactc gttaggcggc gatttgggcc gcaacgccag
      721 gagcgcggcg acgatcggcg ggatgaggat aaagctgcgc agccacggct ccgaatgcca
      781 gggcagcaga aagagccgtt cgtcccaaaa gaggtaataa gcgaaaaccg tgatcgagag
      841 cacctgccac gcgtcggccc ggcgccacgc ggcgagcggc aggatccagg tgcaatacca
      901 cggatgcaga accggactca gaatcagcgc cgtgcccatc acccagagca ggccgcgacg
      961 ccaattccgc ataaagagga gcgacacgag cacaactgcg acgatgatca cgacgttgta
     1021 gtgataattc ttctggtgcg gattgggcca gaagctctcc tcgatgagcc accaaaacat
     1081 gtcattcaaa cgcgcgatat aaatgaatcg ccctaacgac tgccagatgg gcacgcgcgg
     1141 aaatccaaat ggcagactga gcagggccgg aattcccagg ctgagggcca gcgcccaggc
     1201 gcggcgcccg agcgcgaaaa cgcagaccgg caagatgagc accgggacga gcttgatcga
     1261 aatcgccgcc ccgagcgcgc cggcgccgag cacggcccag cgccattgcc cggccgcatc
     1321 ggtcgcgacc cgactccgga caaaacaaag aatcccggcg agcatcgcca gaatcatcaa
     1381 actgtcgaag tgcgccgccc cggcgaagct gtagacgacg agcggattcc aggcgtacca
     1441 ggcggcatcc gcatagcgcg caggcccgcc gatgaggcgc aagaggaccg cgatggcgcc
     1501 gagatcggcg gcggcaaaaa gcaatttgta gaagagcggg tcggccgcga agcgactcag
     1561 cccggcgaaa atcagttcgg tgcctggcgg atagatcgcg ctgaaatcgc ggtgattgat
     1621 ctgattccaa tccttgaact gcgcccgcac cggcgccaac tgttcgtcgt taggcgaaaa
     1681 cacgtaagga ttgaaacccg cgttctggac tcgcccctcc cattggtagc gccagatatc
     1741 gtcccccggt tcgagcggga gcgccacggt tcgcaggaga atcgccacgc tccagaaaat
     1801 cactgctccc atccggccca acggcagttt ggcgaaggcg gacgcggccg caaaaaagcc
     1861 gatcccgcag agcaccgcgg tctcgacaaa tttgaccggc atcgtggcgt aatgccagtc
     1921 atcaaaacgg tggagcgccg cgagaagcag gatctccgcg aggagcgcga gccaaaacca
     1981 cgcctgccta acgatcggca tcgggcgtcc tttttcttgt gatccgttgc aaaccgaggc
     2041 ggtaggcggc gacgtcgacg atttccgcca cccgccgctt caaacgccgg gctcgcgttc
     2101 ccagaaaaag caggaagatg gcgtagggaa ccaggatccg cagatcgacc ccggcgctgt
     2161 tcaggtcgcg gtagaggatc atcgcgagga cgagcagatt gaggatgacc gtcgtagtca
     2221 caaagcggta gcggagccgg actctcgtca ggcacttcgg tccgccgtga tattccgtca
     2281 cggtctggag gacgacgctc caaaagaaat ttccgtagat ttggatgtcc cattctttcc
     2341 agccggtgtc ggccgagtag ctccagcctt cttcttcaag gagctggaag atcgatttga
     2401 gaagtgcatt gcgctcgacc cccgcctcgc tccaatagtt gcgccgccgc agactccccc
     2461 ggcccgctgt tcccgaaggc attttctcat gcgtcccgat gacgccgcgc ggggtgcgct
     2521 tgaattccag ccaggtgaaa tagcggttcc aaccacgcac cagcggttgc gcgaaggcga
     2581 gaaacatgac gagcaatcgc gccgccaccg tgtcgaacct cggctcaatc cgcgcccgca
     2641 gcatgtagga gagcgcgacg cagagcgtgc cgcccagcat caggtagggc acgatgcgca
     2701 acacgggcag gaagattccc agcccgaaaa gaaaaagcgt gagcacgaac cactcgatac
     2761 tgctgaggta gttggcgatt tccgattgcg gcgacggata gatcgattgg aaaaatcctt
     2821 cgccaaaaat cccgtgataa atgatcgggc ggttgatgaa ccaactgaaa cggggcgagc
     2881 cgtagatctg cccgcgccat ttcgcggtgc ctgtcgggcc aaagaaaata aggtgcttga
     2941 aacgcagcat cgattcggcc tcgccgtatc cggcctgctg tttgcgaaac gcgcctaacg
     3001 taaagcggcg atgatgccag acgatcgcgg tcggactaaa agcgatgacg tggccttcct
     3061 gttgcaggcg ccagcagaaa tcgacgtcgt ccccggcctt gcgatactcg atatcgaagc
     3121 cgccgaccgt gtcgaaagcc cagcggtaaa aagccatgtt acacccggga atgtgctcgg
     3181 cgacggtgtc ggtaagaagc acgtggctcg ggccgcccgg cgccgccgcg acacaggcct
     3241 gcacccagtt ttccgccggc ggcgaaacgt tcggcccgcc gacgccggcg tagtcgccgc
     3301 tcacgagcgt gccgatcagg taatagagcc agtcgggatc cgccatgcag tcggagtcgg
     3361 tgtaagcgag gacctggcct tgggcggcgg cggcgccggc gttgcgcgcg tagctcaagc
     3421 cgtgattctc ctgccgaatg tagcggacct tcgggtagcg agccgcgatc tccggcgtgt
     3481 tatcggtcga gccgtcgtcc accaggatcg cttcgtaggt cggataattg atcttgccta
     3541 acgactcgag gcattccgcg agcgttttgg ccccgttgta ggaacagacg atgactgaga
     3601 cgaaaggcgt ttgcggcaaa tcgcggtgcg ggagcgaggc gtcgtcctgg ccgagcttct
     3661 tctgcaacga gaagaacgac ttcttcggcg tccggtcccg tcgcacgagc ccgaaggacc
     3721 aatctgtaat ctcggtttcg ccggtaaacc attcatccgt ccaggcgaaa aagatcgtcc
     3781 cggcaagacc gcaacggacc acgctgtcga cgtgccagcc gagcatttca gcctgctcct
     3841 cctcgctatg gcggatcgtg tccatgccaa attcgccgag cataagcggc ttttcttccg
     3901 ccaggttttg caggcgcaga aggtattttt caaaatcgcg ctggtcgtgc aggtaaacgt
     3961 tgaaacagta gaaatcgacg ttctgcggga ggagatactc cgtcggcgga taactggcgt
     4021 aggagaagag gacgcttgga tccgcttcgc ggccgacgcg aatcagggtc tcaagaaatt
     4081 ccgtcacgcg acgcacaccc agccagcgga ccatcgtggt cgggatctcg ttgccgacga
     4141 gatagccaaa aatggccgga tggcccgcct ggagcgcgac cgccgcgcgc accgcccgca
     4201 cgatctcgcg ccgcgttttg gcccgaacga gaaattccac atgcttcgcc cagggaagcg
     4261 tgatcaacac ccgcacgccg gcctcggaac aagcatcgag gaaccagcgc ggcgggagat
     4321 gatatgtccg cacgacgttg atacccaggg cacgcatctg cgtgaggtcg cgccgcgcct
     4381 gttcttcgct gccgagataa tcccccgctg cgtccggcct gaagggcccg taagtggcgc
     4441 ccttgacgaa gaacttccgg tcgccctcga agaagaactt ggcgagagat cgaacgcgga
     4501 ccggcaaagt ctgactcacg aggagctttt cctagtgcag ggcggtggcg gacgcaaaac
     4561 ttttgcgcgt cccgcgccta acggctgagt cgggccagtg cctcgcggac cacccgggct
     4621 gtcgcttcga tctggtccgg ccgatgcgcc gtagagataa acccagtctc aaactgcgac
     4681 ggcgcgaaat aaattcccgc ctcgagacaa tgagtgaaga aacgcgcaaa tttttcgagg
     4741 ttgctccgtc gcgcgccggc cagatcgttc accggttcgt ccgcaaaaaa gaggcaaaac
     4801 atcgagccga tacgatggaa aacagccgga gtcttcgttt cgccgatcgc atcgcgcatc
     4861 gcctcttcga gttgcgcgcc gagcgtttcg agcgccttcc aaccgtcggt gcgttcaagc
     4921 tcccgcagtt gcgcgaggcc cgcggccatg gcgagcgggt taccggaaag ggtgccagcc
     4981 tggtaaaccg gcccgagcgg cgaaagtcga tccatgatct ccgcgcgacc gccgaaagcg
     5041 cccaccggca ggccgccgcc gatgactttg ccgagcgcag tcagatcggg ccggactccg
     5101 tagacttcct gcgccccgcc acgcgcaacg cgaaacccgg tcatcacttc gtcgaagatg
     5161 aggagcgcgc cgtgctgggt gcattcctcg cgcagcgccg cgaggaagcc gggctgcgga
     5221 aagtagaggc cggcattggc cggaatcggt tcgaggatga tcgcggcgat ctcgtttgga
     5281 ttcgcgcgga gcgtctcgcg cacggcctcg atatcgttga aaggaagggc gatcgttagg
     5341 ccagcgaacg cctccggcac cccggcgctg tcgggctggc cgtgcgtgag ggcaccgctg
     5401 ccggcgcgga cgagcagaga atcaacatgc ccgtgataac agccctcaaa cttgatgatc
     5461 ttgtcgcgtc cagtgaaacc acgcgccaac cgcacgcacg acatggtcgc ctccgtcccg
     5521 ctgttcacca tccgcacctt ttcgatcgag gggacccagc ggcagattaa ttccgccatc
     5581 tcgacttcca gcgggtttgg aattccgaag ctgaccccgc gaccggccgc ggcgcgaatc
     5641 gcctcgatca ctgccccggg agcgtggccg agaatcgcag ggccccatgt gccgacgtag
     5701 tcgatgtaca ttttgccatc gacgtcccag atgttggcgc cggccgcgcg ctccacgaaa
     5761 aacggctcgc gaccgagacc acgaaaggcg cgcacggggg aattgacccc gccagggata
     5821 aggcggcagg cgcgggcaaa gagttcagag gactgcgcag gcatcgaact aacggtcaac
     5881 gtgcaacgta caacgttcaa cgttgaatgg atggagcccg tttgttaact acctcctgcg
     5941 cgcattccta cccctggctt cgcaacgacg gcggagcttc gtctcctggc taaaaatcgg
     6001 ctttttcccg gccgaggtca gaacaatgct gcggaaccct ctggaagagc gacgagggtc
     6061 tgatttactc tgaaaagcag aatcactgcg gtcctcccct gatcttgaag acgtttccgc
     6121 tgtaatcggc gaggtagatc tcgccgcggg cgtcttctcc cagcgaagtt agcgagccta
     6181 aagtgtaacc gccaatccgc gtgggaaaca gttggaagtg atctcttgga aattggaagc
     6241 gaccccctcc cccgataaac caaggtgaag actctgccca aagagttctg gggatccggg
     6301 ccgaacaatc accgaagaca tagaggccgc gcagatcgtg cactttcgtt ccgcgatagg
     6361 atcgacagga aacacgcgga gacgcaattt ttgatattac gcgtcaaggt gggccgcgga
     6421 gttgtgttgg ggtttcgtag gattcttaac ccctacggga gcctagccag gtcttaaagt
     6481 gaagcttttt actaaggttc ttccctgaat tgccagcaaa gaacttttaa ccgcggactt
     6541 tccgtgcgaa gcacgccggt ccagagggga ccaacgttga cacccccaga gggcgcgcct
     6601 acgctccggt tcgcaaatcg gcggggtagc caagtggtaa ggtcggggtc tgcaaaacct
     6661 ctattcgcgg gttcgattcc cgcccccgcc tgttttcaga ggaaaatcag cgcaaatcag
     6721 ggcgagaaaa tgcctaattc agaacagaaa tcagaacacg tccactgatt tcagccgtgt
     6781 ttgccgaagg tcgctttagg ctcctccgac gaactgcacg agcgagtggt tcgccagaac
     6841 gttgattgag aaaaggccac cgggcacagg ctgattttcg agtccgcaat tccgcctggc
     6901 gacgtaatcg aaccgttcca ggttgcgggc gctgtcgaga atctcggccg gctgcattga
     6961 ttcaaggtgc ggcaagacct tgtctgtgac gcccgccatt cgtgctacgt gccgttctgc
     7021 gcgctcgcgc atcgtgattg cggctgattg catcggggtg attgcattga ttgcatcagg
     7081 cttagccaat tctcgggcca ggtccgggcg ctcaatcaat ttctcggttg cgatttgctg
     7141 cgtccagcgt tcgcgcttgg cgcgagcgag cacagtcccc cgctgggatg cccatgttgc
     7201 gggccagttc gcgcagaccg atgccgctgg cgtatgcagt ccggatttcc gcccaggttg
     7261 atgctgggat gcggctcatg ttcgagtgaa cgtaatctgc
//
LOCUS       DATMNS010000214         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1232179, whole genome shotgun sequence.
ACCESSION   DATMNS010000214 DATMNS010000000
VERSION     DATMNS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232179"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..648)
                     /locus_tag="VNW28_07435"
     CDS             complement(16..648)
                     /locus_tag="VNW28_07435"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HXA09799.1"
                     /translation="MKQILVSCVFALLVSSTARAGTEEPVQTTETPEVDSNFAKGSKE
                     FQNVAGAFFFFDRGGNNRPSVDLAVDSLRLGLMLSNPHGTSLLAGNFELLGEVFAGPI
                     FEGPGNVTAGATLIIRYNFIQPRARLIPYLQIGAGGVYTDISEKASGGLVSLPVEFNL
                     QGVGGTRLMLNDRWSMVIEAGYRHISNATIKLPNYGVDSVGGNLGFGFFF"
     gene            complement(744..1631)
                     /gene="rfbD"
                     /locus_tag="VNW28_07440"
     CDS             complement(744..1631)
                     /gene="rfbD"
                     /locus_tag="VNW28_07440"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:TIGR01214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HXA09800.1"
                     /translation="MNAKPKIVVVGAGGRLGAALAREYASRDETIGFTHAQLDLGQPE
                     VLRRTLASLPFDVLINAAALTNVDYCEDHREEAMRLNAEAPRVLAEICGEKEARFVHV
                     STDYVFDGEKRAPYTEQDPADPISVYGESKRAGEERVLAANDRALVIRVSWVFGPDRA
                     SFVDAILKKARAEEQVAAVADKFSTPTYTPDIATLLRPLLDSAAVRGIVHLANRGACS
                     WQEYAQWALDCCHEFGVPMKARTIAAASLGEMTNFVARRPVYSVLATGKYEQLTGETP
                     RPWRAAVADYVREHYGKGK"
     gene            1807..2664
                     /locus_tag="VNW28_07445"
     CDS             1807..2664
                     /locus_tag="VNW28_07445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXA09801.1"
                     /translation="MRAIWKGSINFGLVYIPVSVYPATKEEKISFRQLRATDLSPIRY
                     KKVAEADQKEVPADQIVKGFEYEKGKWVTLSDEDFEKVQIESTHSVEITDFVEQAQIN
                     PKFFYKPYFLEPQKGGEKAYALLHKALSTSGRVGVAKVAIQKREYLAAVKPDGLFLIL
                     ELMHFAQEVLEPASLNSAEDKTVSPQELKMATALIDAMTTEWEPTKYKDQYQTALMQL
                     IEEKAQKRAPAAKPPAPRVQPNVIDMVAVLQQSLQRASHQKKSSAGRSAATKSTAALV
                     KRKRRSAAA"
BASE COUNT          611 a          868 c          791 g          494 t
ORIGIN      
        1 ttttcatagg attgtctaga aaaagaagcc gaaaccaaga tttccgccaa cggaatcgac
       61 gccgtaattc gggagcttga tggtcgcatt cgaaatgtgg cgataccccg cctcaatgac
      121 catcgaccag cgatcgttca gcatcagacg cgtaccgccc acgccttgga gattaaactc
      181 gaccgggaga ctgacgagtc cgcccgacgc tttctcggag atgtcggtgt agacaccacc
      241 ggccccgatt tgcaggtagg gaatgaggcg cgcccgcggc tggatgaaat tgtagcgaat
      301 gataagggtc gcgccggcgg tgacgttgcc cgggccttca aagatggggc cggcgaacac
      361 ttctccgaga agctcaaaat tgccggcgag gagactcgtg ccatgcggat tgctcaacat
      421 caaaccgagg cgcagactgt cgactgcgag atcgaccgaa ggccggttgt tgccaccgcg
      481 atcgaaaaag aagaaggcgc cggcgacgtt ctgaaattcc ttcgacccct tggcgaagtt
      541 actgtcgacc tccggcgttt cagtggtttg gaccggctct tccgtaccgg cgcgagcggt
      601 gctactgaca agaagtgcga aaacgcagga gacgagaatt tgtttcatct gcgcgcgata
      661 gaacgccaac ccggaagtat tgtcaaccgg cttggagacg ctgaaggaag ccggacggca
      721 actcgcggaa tcgggacgcc cggtcacttc cctttgccgt aatgctcccg aacgtagtcg
      781 gccaccgcgg cgcgccatgg gcgcggcgtt tctccggtca gctgctcgta tttgccggtc
      841 gccagcacgc tataaaccgg tcggcgagcg acaaaattcg tcatctcgcc taacgacgcc
      901 gcagcaatcg ttctcgcctt catcgggacg ccgaactcgt ggcagcagtc gagtgcccac
      961 tgcgcatact cctgccagct gcacgctccc cgattcgcca gatgaacgat cccgcggacc
     1021 gccgccgaat cgagcagtgg tcgcagcaaa gtcgcgatgt cgggcgtgta ggtcggcgtg
     1081 gaaaatttgt cggcgacggc cgccacctgc tcctcggcgc gcgctttctt cagaatcgcg
     1141 tccacgaaac tcgcgcgatc cggcccgaag acccacgaga cgcggatgac cagcgcgcga
     1201 tcgttcgccg ccaggacccg ctcctcgccg gcccgtttcg actccccgta gacgctgatc
     1261 gggtcggccg gatcctgctc cgtgtagggc gcccgctttt ccccatcgaa gacataatcg
     1321 gtgctgacat ggacgaatcg cgcctccttc tctccgcaga tttcggcgag cacccggggc
     1381 gcctcggcat tcaacctcat cgcctcttcg cgatggtcct cacaataatc cacattcgtt
     1441 agggcagcgg cgttgatcag gacgtcaaag ggcagggacg caagcgtcct gcgcagcact
     1501 tccggttgtc ccagatcgag ctgggcgtga gtgaacccga tggtctcgtc acgcgatgca
     1561 tactcccggg ccagcgccgc ccccagccgt ccaccggccc ccaccacgac gattttcggt
     1621 ttcgcattca cagaatcaaa aagacatgct ggtcaatcaa gctgatgaac cacaggtgtg
     1681 accggcaagc aaggtgcgac cttgcaacct gccagtaagc cacccgctct tccgggcaac
     1741 agacttcctg gccagcgcgt cgttagtctt gacatctggc ccgggaatag ctgacgttcc
     1801 taaactatgc gcgcgatctg gaagggcagc atcaatttcg gtctcgttta catccccgtc
     1861 tcggtctatc cggccacgaa ggaggagaag atcagtttcc ggcagctccg ggcgacggac
     1921 ctgagcccga tccgttataa aaaagtggcc gaggccgatc agaaagaagt ccccgccgac
     1981 caaatcgtca aaggctttga atacgagaaa gggaaatggg tcacgctgag cgatgaggat
     2041 ttcgaaaaag tccagatcga atccactcac tccgtcgaga tcaccgactt cgtcgagcag
     2101 gcgcagatca acccgaagtt cttttacaaa ccctacttcc tcgagccaca aaaagggggc
     2161 gaaaaagcct atgcgctctt gcacaaggcg ctcagcactt ccggccgagt cggggtcgcc
     2221 aaggtcgcga tccagaagcg cgagtatctc gcggcagtga aacccgacgg tctctttttg
     2281 atccttgaac tgatgcactt cgcccaagag gttctcgaac ccgcatcgct caacagcgcc
     2341 gaagacaaga ctgtctcccc gcaggaattg aagatggcga ccgcgttgat cgacgcgatg
     2401 accacggaat gggagccgac caaatacaaa gaccaatatc agaccgcgct catgcagttg
     2461 atcgaagaga aggcgcagaa acgcgctccc gcggccaaac cgcccgcgcc ccgcgtccag
     2521 ccaaacgtga tcgacatggt cgccgttctg cagcagagct tgcagcgcgc gagccaccag
     2581 aaaaaatctt cggctggtcg gtcggccgcg acgaagtcga ccgcggccct cgtcaagcgg
     2641 aagcgccgat ccgccgcggc ctagcgttgg ccctcgtttc gcctggcggc ccgcgcgggg
     2701 cgaaatcacg cggcaaatac ttctcgctcc ggccccgaag aacatgacgg cagggtaaca
     2761 gaat
//
LOCUS       DATMNS010000215        24156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1738629, whole genome shotgun sequence.
ACCESSION   DATMNS010000215 DATMNS010000000
VERSION     DATMNS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 24156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24156
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738629"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..5937
                     /locus_tag="VNW28_07450"
     CDS             121..5937
                     /locus_tag="VNW28_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HXA09802.1"
                     /translation="MEPVRAGCIEIRGARQNNLKGIDLDLPLGQLTVVTGPSGSGKSS
                     LAFDTIYAEGQRRYVETFSPYTRQFLERMDKPRVDEIRGIPPAIAIEQSNPVKSSRST
                     VGTMTEINDYLKLLMPRVARAFCPSCGREIRPETAKSIADEIMAQSPGKTGLITFWVG
                     VPAKTEPADFFAFLQQQGYLRVWLNGEVVRADSENRKVRLGARVQVIQDRLTISEEGR
                     IRLVESLETALRFGKDKINFVDLATKAEYPFSTGWHCAHCDLDIRPPTPGLFSFNNPL
                     GACPTCRGFGRTIGLDLDRAIPDRSLSIAQGAVRVFRGEEMGESQKDLLRACAREEID
                     VHLPFEELPKCDQDFVINGEKGAGEFSEEDYENDRWYGVRGFFRWLESKTYKMHVRVL
                     LSRYRAYTTCPRCRGGRFQPETLNYRLMTDDGVAFTLPEFAALPIGQAREFLSALALS
                     PNDSAAGMLRAEIVARLRYLCEVGVNYLTLDRATRTLSGGEVQRVNLTTCLGASLVNT
                     LFVMDEPSVGLHPRDVGRLVRVMHDLREKGNTLLVVEHEEAIIRAADHLLDLGPGRGE
                     SGGELVYSGRLADFLGSARDPRAGRGDSPRTSVRADPDRRVADQGTRVACAPQSLTRE
                     YLTGKKSIPVPKMRRPSAAAIRIKGAAEHNLKKIDVDIPLGVFTCVTGVSGSGKSTLI
                     HEVLYRNLLLARGLAGDEAGACKSVTGAHRVGEIVLVDQAPLSRTPRSTPILYLGVYD
                     RVRELFAGLPEAMAQGLTASAFSFNSGSGRCERCAGTGFEKIEMQFLSDLYVRCAECE
                     GRRFQPHVLKVKLAGRSIHDLLELTVSEAVQFFTALGEAERLAQPLAILEEVGLGYLR
                     LGQPLNTLSGGESQRLKLVKHLAETGNRKPETGDLFIFDEPTTGLHFDDVALLVRLFQ
                     RLVEAGNSIVVIEHNLEVIKCADWIIDLGPEGGEEGGRLVATGSPEDVAKIRASHTGQ
                     FLRRLLGSAGDSPKTAQRYALPNDDEEILRAAEEPCGESRIPQWRDGSRALPGVLATP
                     HHHAIAIHGAREHNLKNISLAIPRDQMVVITGLSGSGKSTVAFDLLFAEGQRRFLDSM
                     SPYARQFVEQLEKPDVDLIEGLPPTVAIEQRVTRGGGKSTVATVTEVYHFLRLLFAKA
                     GTQYCPDCDLPVEKQSVAAIVRQVETAARRGPLKVLAPLVKARKGFHTEVARWAERQG
                     FDTLYVDRALIPVARFRKLERFKEHTIDAVIGVIDAKRILKARNLVQRALDIGRGTAH
                     LLDAKNRLTVMSTEMSCPGCGRAFEELDPRLFSFNSPHGWCQACAGFGEIWNEKLQQN
                     EEETGESMLENELQAERQFETVDETEAVVCPQCHGSRLNPIARHVRLQGKTIDEFVAR
                     SAGEARGLIGQLRFRGTQKVIAADLVPEIEQRLRFMENVGLGYLALGRSAKTLSGGES
                     QRIRLAAQLGSNLRGVLYVLDEPTIGLHARDNLRLLETLGALREKGNSLVIVEHDEET
                     MRRADHIIDLGPGAGVDGGEVVAEGTLREIEKSAASQTGRCLRTPLCHPVRKSRRSLG
                     EVEDWLEIRGAQTNNLKNVDARFPVGRFSVITGISGSGKSTLMRSVLLPAVRQTLGSA
                     RDSRAVFGDSPKTSRGKKVRAGEVAIAGTRVACAPQISGADALEAVYEVDQSPIGKTS
                     RSTPATYIKVFDEIRNLFAQLPVSRVRGYSASRFSFNTEGGRCETCGGQGVLKLEMSF
                     LPSSYVPCEDCGGRRYNPQTLEVLYHDRSIGDVMDMTIGQAAEFFAANPKIARPLSLL
                     VETGLGYLKLGQPSPTLSGGEAQRLKLATKLTRGVGRAQNERLRKMRQPKSTLYLLEE
                     PTIGLHMADVELLLHVLHRLVDDGNTVIVIEHNLSVIAEADYVVDLGPEAGSLGGEIV
                     ATGTPEEVAKNRRSRTAPFLRAVLDRGLLAARERKAAL"
     gene            6077..7009
                     /gene="lgt"
                     /locus_tag="VNW28_07455"
     CDS             6077..7009
                     /gene="lgt"
                     /locus_tag="VNW28_07455"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXA09803.1"
                     /translation="MLAYYLHDFDPFIFHVWGDFGPRWYGMAYVAAFACGFWLLRVLA
                     QRGYTQLPAAQVGDFITWGALFGVMLGGRLGYVLFYRPDMLRDPLSIVRVWEGGMSSH
                     GGMLGLIAFTFWYAWRHKLPWTNLGDNLVVVAPIGLFFGRCANFINGELYGRVTTVPW
                     AMLFPKELLEPENAAEAERALLATQQVDPRLTTPEALVQGLHAEPQLRAVLRPILTPR
                     HPSQIYEALLEGVLLFTILWLVRTRTRQPDGVLTGLFFVCYAIFRIVVEYFREPDASL
                     IAGFTRGQFFSFFLIAIGLAFIIAGKIRNRRTAL"
     gene            7165..7929
                     /locus_tag="VNW28_07460"
     CDS             7165..7929
                     /locus_tag="VNW28_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXA09804.1"
                     /translation="MTDLFFPGFLAAGGLVFSFEHSTVAGKMVLLLLAIGSIFSWSVM
                     VTKMRVVQFARKQSDRFLAAFRQDRQPLRLFESNVRFPGSPVFQVYRAGCEELTFHLL
                     GSAELDETFRARLEIADKITPAQMHAVTAAMERAVGETALKLESQMILLATAVSGAPF
                     LGLLGTVWGVMDTFTGVAEAGSANLAAMAPGVSGALITTVTALCVAIPAMFGYNFLVT
                     SIRGMIVEMDNFAAELASEFEHKYVEHGGRVSDFRR"
     gene            8030..8446
                     /locus_tag="VNW28_07465"
     CDS             8030..8446
                     /locus_tag="VNW28_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXA09805.1"
                     /translation="MRRYSNRSSLTTLSEINITPLLDLAFVLLIIFMITTPLLENSKS
                     LIIPSSATTNAPINPAAVQTISIDRDSIIRLNEEEMDAATLESRLVELKRAMPEVAVV
                     IRPDRELPVQKLVSLMDALQRADITKVGIATQEEQK"
     gene            8449..9357
                     /locus_tag="VNW28_07470"
     CDS             8449..9357
                     /locus_tag="VNW28_07470"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXA09806.1"
                     /translation="MTRDARFWRNVTIVALIHVVVLLGLLWWSRTPTRVPKDVVWMEG
                     GAGTMAAAAETPAATPESAPVETPEPAQEEPTSEVAPTAKSEIEIPAPAATATPTPTP
                     TATPKPTPTPTAKPTPRPTPKPTPKPTPKPSATPKKKATPKPKPKPTPDKEKEAAAKE
                     LEKKKAIAKAALAKEKSDEGEEGSPEKPVKKGTAGKGGSGGKGSDAGGASEFGWYGNM
                     LHDRFYSEWVQPTSVVHSATLSVLLRIRIQKDGRISDYSIAKSSGNPVVDDSVTAAAA
                     RVKRVDPLPSGLGDDYYDVNINFELN"
     gene            9361..10545
                     /locus_tag="VNW28_07475"
     CDS             9361..10545
                     /locus_tag="VNW28_07475"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HXA09807.1"
                     /translation="MRRQRISDFKFQISSVMVTALLFGLTTGSARAVQDVPTITVSKG
                     DQINLAVSALGGGEGAAVTKVVQNDLALSGYFTFGGANSSYTVRGTASGGSLQGQVVD
                     HSGGTVLAKTYGGSTRENAHQFANDVIETLTGQKGIAGSKITFIATRSGKKEVYLADY
                     DGSNVRQLTHDNTISVHPSLSANGRRIAYTGYQSGYADVYVIDLASGARNRIVNYPGT
                     NSGATFSPDGSRLALTISKDGNPELYTVSASGGGARRLTHTRGVESGPTWSPDGDEII
                     YSSDEGGSPQLCRISASGGVGRPISTGRGYCTEPNWSPDGKKVAFNVRSGGEFQVAVL
                     DLPNGGTRIVGVGQDPVWGADSRHLIFSEGGALYLLDTVSSRKSKIVDGLGKITEPSW
                     SR"
     gene            10636..11835
                     /locus_tag="VNW28_07480"
     CDS             10636..11835
                     /locus_tag="VNW28_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006984027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXA09808.1"
                     /translation="MKAGFLEKLIGRLGRIGPEDVQNYLLRLAEEKGFLETIFNAIQE
                     GIIVTDSNGRITYLNHAAGEIFGLDGENAIGKPLAEQVRGLDWQALSHSDTVLSRDME
                     IFYPANRFINFYVVPLMIERRSPNEEPDELAEAVGHAMILRDITESRRSTQQTIESER
                     LSALTLLAAGVAHEIGNPLNSLHIHLQLMERKVKKLSGAEDKRALQESIDIARAEINR
                     LDSIVTQFLRAVRPSKPQLHPENVNTIVQEAVRFFRPEIKDRDIVVETELRSDLPPLE
                     LDRDQMKQAFYNAIKNSFEAMKRHGILRIRTDRDDTHVKVSFTDTGGGISAENLSRVF
                     DPYFTTKSGGSGLGLLIVRRIVREHGGELAIESAEGKGLTLTIRLPFLDRRVRMLPDR
                     EKMKDEG"
     gene            12092..13516
                     /locus_tag="VNW28_07485"
     CDS             12092..13516
                     /locus_tag="VNW28_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXA09809.1"
                     /translation="MQPTVLIVDDEKHTRDGLRSLLGEHYDTYVAADVAGALDVLERD
                     QIDLLLTDLRLGAEDGMTLIERALKLPHPPVCIMMTAYGSVDTAVEAMKRGAYDFVTK
                     PLNLDKVEMLIARALGSRRLEQENRSLRQEVDERFGLENLIGESVALREVLDTIRQVA
                     PSSANILLEGESGTGKELAAHAIHNLSRRHKGKFVTVHCAALSPQLLESELFGHERGA
                     FTGAHERRIGRFEQANGGTIFLDEIGEIDATTQIKLLRVMSEERAFERVGGSQTLHAD
                     VRLIAATNKNLETLVSEGKFRDDLYFRLNVVKITMPPLRERKEDIPLLVRSFLRHFCK
                     ANEKPLLDLTTDAMNALLAYDWPGNVRELRTAIEHGVVMANGAKVTLRDLPAAVRQAA
                     PAAGVRGHAFDEKASAFDLHATEKRLITQALATTRGNITAAAKKLGISRRTLHRKINE
                     MNAAQPNDHHESGKMDASSDDARE"
     gene            13503..15242
                     /locus_tag="VNW28_07490"
     CDS             13503..15242
                     /locus_tag="VNW28_07490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXA09810.1"
                     /translation="MPGSDDAVQRAVHALETGGMAVWIRRALAFLLIVGLALFYLLHE
                     FRGLATSQAMDQAQIGRELLHGHGWKTKFARPLAAGQLQRRGKDVAGKIWTDTYNAPL
                     PPLVDAIALLPVRSHLKMTTRDIVYLGDKMIALLSMVFFLASVVVLFLIACRLFDQYL
                     AVLGCALVLLGDTFWQYSLSGLPQMLLLLLFNLTLYVLVRAIEARAEGRAVLRLLGLA
                     GAGFGLLALSHALTIWIFLPTLIFAALYFRPRLWAAIWMLAPFLLLYTPWLLRNYLVC
                     GNPAGVALFSFFDQIGMSEAGHMRHLAIDYQGMSIGVLRDKISDNVAGQASRILQYFG
                     WSVVALFFFAALLHRFRRAVTATTRWFVFVMWGGAVVGMAVYGVNEEQGVAANQLHLL
                     FVPIMTCFGLAYLLVLWNRLQITSRLARFAFLTLFFVLCGGPMIFNLLFASAKTGVRW
                     PPYVAPYIAVINDWMKPEEITATDMPWAVAWYADRRAVWLPDTIRDFTDMSDYDLLGG
                     KINGLYLTPISGSQNTLKNIVEGEYKGWAPVILRSVDLKKFPLPWATLLPLDDECIFF
                     SDHDRRKASTDQP"
     gene            15242..16624
                     /gene="glmM"
                     /locus_tag="VNW28_07495"
     CDS             15242..16624
                     /gene="glmM"
                     /locus_tag="VNW28_07495"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXA09811.1"
                     /translation="MAAAKKIFGTDGVRGVANVEPVTAETALKLGRAAAHVFSALNPQ
                     AHPSGLRPKIVLGKDTRLSGYMLENALVAGITSLGVDVLLIGPLPTPGVAYITRSLRA
                     DAGIVLSASHNSYEDNGIKFFRHDGYKLDDAIERKIEQLVFTGEIDSVRPTAKKIGRA
                     TRIDDALGRYVEFAKASFPRGMSLEKMRIAVDVANGAGYKSTPCILGELGAELSIAHN
                     QPNGTNINAHCGSVYPQEIQRLVRESGATVGIANDGDADRVLLCDERGEVVDGDEILA
                     MASVDLLRNGQLQENTLVATVMSNFGLDEALAAHGGKVVRTKVGDRYVIEEMVRQNLN
                     LGGEQSGHIIFRDFTTTGDGIISALQILRIMQATGKPLSELKKCLTKYPQAQRNLLVR
                     EKPPLETLPEVMKLVQDTEKELRGNGRVLLRYSGTEAKIRLLIEGRERTQIDHQADRI
                     AGAIQSAIGL"
     gene            16781..17431
                     /gene="scpB"
                     /locus_tag="VNW28_07500"
     CDS             16781..17431
                     /gene="scpB"
                     /locus_tag="VNW28_07500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HXA09812.1"
                     /translation="MNLPQVIEALLFSAQRPLSTRELVAAIRGAGAADEFTPNEFAKT
                     TEAAVAAALEQLKIEYLEQQRAFQLVEKAEGWQLASDPQFARWVRQLFPAAKPARLTP
                     PGLETLAIIAYRQPITRADVEAVRGVAIESVLQTLMERGLVRIAGRAEVPGRPLLYET
                     TQFFLEHFGLRNLDELPNSEELKRRELPRAPMAPAEHGAAAESAPEQGEKIASEPA"
     gene            17519..18178
                     /locus_tag="VNW28_07505"
     CDS             17519..18178
                     /locus_tag="VNW28_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXA09813.1"
                     /translation="MKTFAFGCGGILLVLVLVAVVVGIAVAGKYNGLVKLDQSVNQSW
                     AQVQNVYQRRADLIPNLVQTVAGAADFEKSTLIAVTNARASVGQVKLDPNKAPTDAAQ
                     LEQFQAAQGQLSNALSRLLVVSERYPELRANQNFLALQAQLEGTENRISVERGNFNTT
                     VQAYNTAVRTFPTNLFAGMLGFQPRPFFNAQPGAEKAPEVKFNFGNTAPAPAATAAAT
                     P"
     gene            18266..19030
                     /locus_tag="VNW28_07510"
     CDS             18266..19030
                     /locus_tag="VNW28_07510"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HXA09814.1"
                     /translation="MKTRLLPGFFALFVCATGPLAAERMPSKPDRYFNDYAAAVDPAT
                     AAQLNEQLAQFERETSTQILVAVYRSMESESSVADYTQRIAQTWGVGQKGRSNGAVLF
                     VFVDDHQMFIQTGYGLEGALPDATCFDITHNLIAPFFKKGDYAGGLKAGVAAMMQAVK
                     GEYKGTGRTQKESKTGGSSGMPVGLIVFLIIVAFVIFANRSRSRGYGYSGFGGPFIGG
                     FGGGGWSGGGGSGGGGFSGFSGGGGSFGGGGAGSSW"
     gene            19035..19472
                     /locus_tag="VNW28_07515"
     CDS             19035..19472
                     /locus_tag="VNW28_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5130 family protein"
                     /protein_id="HXA09815.1"
                     /translation="MRTKHFLNKIEHARIVQAIAAAEKKTSGEIRVFIQHGAMDDAVA
                     AARAKFQKLGMTATRERNGVLIFVAPRAQKFAVIGDEGVHQRCGDVFWQQLVASMQTH
                     FKAENFTDAVVHAIERTGALLAEHFPREPGNHDELPNAVEEGL"
     gene            19828..20829
                     /locus_tag="VNW28_07520"
     CDS             19828..20829
                     /locus_tag="VNW28_07520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXA09816.1"
                     /translation="MPKTLVKDEESSTETYAKFVAEPFEAGYGHTVGNSLRRVLLSSL
                     EGAAITAVKITGAQHEFGTLPAVVEDVTDIVLNLKRVKFKASDHEARKLSLNVNKEGT
                     VTAGDIQLTQGYEVLNPKQVICTLDKKHKFEAELDVRVGRGFATGDENKRPDQAIGLI
                     PIDSIFSPVTRVKYAVENTRVGQRTDYDKLILEVWTDGRLTPDDALLQASAILRHHLD
                     VFVNFNEDVIEFDETPAGVSEENVKLKKLLNMSVNEIELSVRAANCLNNANITTVGQL
                     AQKTEAEMLKYRNFGKKSLNEIKDKLQQLGLGLGQKFDPGLVEIPLPADGANGADEE"
     gene            20863..21369
                     /gene="rplQ"
                     /locus_tag="VNW28_07525"
     CDS             20863..21369
                     /gene="rplQ"
                     /locus_tag="VNW28_07525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXA09817.1"
                     /translation="MRHQKKTVKLGLTAEHRKALLANQVCSLIEHQRIKTTLAKAKAV
                     RPLAEKMVTLGKNGSLHARRTALAVLRQKDAVKKLFDEIAPRSASRQGGYTRIIKLGQ
                     RKSDSAAVAFLEWVDAAEVVLEETTPAQKGRKRKAAKGEASPEGDAKPSGKAKKTEPN
                     EKETGAKE"
     gene            21575..21790
                     /locus_tag="VNW28_07530"
     CDS             21575..21790
                     /locus_tag="VNW28_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09818.1"
                     /translation="MTHLPNAKPVHEESTYQLLIQSEEKERGLMEAIVYLLLVIATTT
                     TIWQFSHVPVTFADLGASTQNSITAAL"
     gene            complement(22001..22456)
                     /locus_tag="VNW28_07535"
     CDS             complement(22001..22456)
                     /locus_tag="VNW28_07535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXA09819.1"
                     /translation="MGPKAVRIFVLADTHNHLPENLAALAEGADEIWHLGDLCAPSLL
                     VELENLGPPVTLVRGNCDSNFEWPLVVDLQRGDLRFRLVHIPPSQPPENVDVLLHGHT
                     HMPRNERRGGVLFLNPGCVTRPDRGMVPSVATLEIEADGKLIWHLTRLR"
     gene            22543..23760
                     /locus_tag="VNW28_07540"
     CDS             22543..23760
                     /locus_tag="VNW28_07540"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXA09820.1"
                     /translation="MSFNNFGLSPEVVRGTQAMGFSEPTPIQLRAFPIILSGKDLIGT
                     AQTGTGKTAAFALPIMTLLAHHGTFRCLVLEPTRELAAQVETAFRDYGRFTDLRVSVV
                     HGGVGYGKQKEEAAAGTDVVCATPGRLLDLLEQRAMNFRNVNILVLDEVDRMLDMGFL
                     PDVRRIVEQISTDRQTLLFSATLPPEIERLAAWVLREPELVEIGARRAPAETVTHAVY
                     PVAAEQKFDLLIALLERTNYDSALIFSRTKHGADRIASQLKQARHSVAVLHSNRTQRE
                     RVEALEGFKSGKYEVMVATDIAARGIDIAGVSHVINYDVPEHPEDYVHRIGRTGRAQN
                     VGDAFTLMNGGELPSLQAIERFIGQKIPRLKLEGFPYIYTVLFEPESGAGGKRAIGGG
                     RTHRGYSFGRRRR"
BASE COUNT         4709 a         7433 c         7520 g         4494 t
ORIGIN      
        1 gcattgtttt ctccctcgtc gttaggcact tattcgtccg aagtttgcgc tttcggccgg
       61 cagaatcgat gctcgtcggt ggcttccacc agcactgctc aatcccgcct ctccgccctt
      121 gtggaaccgg ttcgggctgg atgcatcgag atccgcggag cccgccaaaa caacctcaag
      181 ggaatcgatc tggacctgcc gctggggcaa ttaaccgtcg tcaccggacc gagcgggagc
      241 ggcaaatcaa gtctcgcctt cgatacgatt tacgcggaag gccagcgtcg ctacgtcgag
      301 actttcagcc cttacacgcg gcaatttctc gagcggatgg acaagccgcg ggtcgacgaa
      361 attcgcggca tcccgccggc aatcgcgatc gagcaatcga acccggtcaa gagctcacgc
      421 tcgaccgtcg ggacaatgac ggaaatcaac gattacctca agctgcttat gccacgggtg
      481 gcgcgcgcct tttgtccgag ctgcggccgg gagattcggc cggagacggc gaagtcgatc
      541 gcggatgaga tcatggcgca aagtccgggt aagacgggac tgatcacgtt ttgggtcggg
      601 gtgccggcga agaccgagcc ggccgatttt ttcgcctttc tgcaacagca aggctatctc
      661 cgcgtctggc tgaacggcga agttgtgcgg gccgacagcg aaaatagaaa agttcgcctc
      721 ggcgcgcgcg tccaggtgat ccaggatcgc ctaacgattt cggaagaagg ccgcatccgc
      781 ctcgtcgagt cgctcgaaac cgcgctccgc ttcgggaagg acaagatcaa cttcgtcgat
      841 ctcgcgacga aggcggaata tcccttctcc accggctggc attgcgccca ttgcgatctc
      901 gacatccgtc caccgactcc cgggctcttc agttttaaca atccgttagg cgcctgcccg
      961 acctgtcgcg gcttcgggcg cacgattggg ctcgatctcg atcgcgccat tcccgatcgt
     1021 tccctctcca tcgcgcaggg cgcggtgcgc gttttccgcg gcgaggagat gggtgaatcg
     1081 caaaaggacc tgctccgggc ctgcgcccgg gaagagatcg acgttcacct gccgttcgag
     1141 gagttgccga agtgcgacca ggatttcgtg atcaacggcg aaaaaggcgc gggcgaattc
     1201 agcgaggaag attacgagaa cgatcgctgg tacggcgtcc gcgggttttt tagatggctc
     1261 gagagcaaga cctacaaaat gcatgtccgc gttttgctca gccgttaccg cgcctacacc
     1321 acctgcccgc gctgccgggg cggacggttt caacccgaga cgctgaacta tcggctgatg
     1381 acggacgacg gcgttgcctt cactctgccg gaatttgccg ctctgccgat cgggcaggca
     1441 cgcgaatttc tgagcgctct cgcgctttcg cctaacgact cggcggctgg catgctccgg
     1501 gccgagatcg tcgcgcgctt gcgttatctc tgtgaagtcg gagtcaacta tctcaccctc
     1561 gatcgcgcga cccgcacctt gagcggcggc gaagtgcagc gcgtcaacct caccacctgc
     1621 ctcggcgcgt ccctcgtcaa caccctcttt gtgatggacg agccgagcgt aggcttgcat
     1681 ccacgcgatg tcggccggct cgtgcgggtc atgcacgacc tgcgcgagaa gggcaacacc
     1741 ctgctcgttg tcgagcacga ggaagcgatc attcgtgccg cggaccattt actcgatctc
     1801 ggccccggcc gcggcgaaag cggcggcgag ctggtctaca gcggcaggct cgcggatttt
     1861 ctggggagcg cacgcgaccc gcgtgccggt cgcggcgatt cgccgcggac ctccgtgcgc
     1921 gctgaccctg atcggcgagt cgccgatcag ggcacgcgag tcgcgtgcgc tccccaatcc
     1981 ctgacgcgcg agtacctcac tggaaagaag agcatcccgg tccccaaaat gcgtcgccct
     2041 tcggccgcag cgatccgcat caaaggcgct gccgagcaca atctcaaaaa gatcgatgtc
     2101 gatattccgt taggcgtttt cacctgcgtg accggcgtct ccggctctgg gaaatcgacc
     2161 ctcatccacg aggtgcttta ccggaatctg ctgctcgccc gcgggctggc gggcgacgaa
     2221 gccggcgcct gcaagagcgt gaccggcgcg catcgggtgg gcgagatcgt gctggtcgac
     2281 caggcgccgc tctcgcgcac gccgcgctcg acgccgattc tttatctcgg ggtttacgac
     2341 cgggtgcgcg agcttttcgc cggcctgccc gaggcgatgg cgcaaggcct gacggcgagc
     2401 gcgttctctt tcaactccgg gagcgggcgt tgcgagcgtt gcgcagggac cgggttcgag
     2461 aaaatcgaaa tgcaattcct gagcgacctt tacgtgcgct gcgcggaatg cgaaggccgg
     2521 cgctttcagc ctcacgtctt gaaagtaaaa ctggccggcc gctcgatcca tgatcttctc
     2581 gagctgacgg tgagcgaagc ggtccaattt ttcaccgcgc tcggcgaagc ggaacggttg
     2641 gcgcagccgc ttgccattct cgaggaggtc ggtctcggtt acctccggct tgggcagccc
     2701 ttgaacacgc tttccggcgg cgaatcgcag cggctgaaac tggtcaaaca cctcgccgaa
     2761 accggaaacc ggaaaccgga gaccggagat cttttcatct tcgacgaacc aaccaccgga
     2821 ctgcacttcg acgatgtggc tcttctcgtt aggctgtttc aacgcctggt ggaggcggga
     2881 aattcgatcg tcgtgatcga gcacaacctc gaggtcatca agtgcgccga ctggatcatc
     2941 gatctcggcc cggaaggcgg cgaggagggc gggcggttgg tcgcgacggg aagccctgaa
     3001 gacgtggcga aaattcgcgc gtcccacacc ggccagtttt tgcgccggct tctggggagc
     3061 gcaggcgact cgccgaaaac tgcccagcgc tacgccttgc ctaacgacga cgaggaaatc
     3121 cttcgcgccg ccgaagagcc gtgcggcgag tcgcgaatcc cgcaatggcg ggacgggtcg
     3181 cgtgcgctcc ccggagttct cgcaaccccg caccaccacg ccatcgccat ccatggcgcg
     3241 cgcgagcaca acctgaagaa catctcactc gccatcccgc gcgaccagat ggtcgtcatc
     3301 accggcctga gcggctcggg caagtcgacc gtcgcgttcg atctcctttt cgcggaaggc
     3361 cagcgccgct ttctcgacag catgtcgcct tatgcgcggc agttcgtcga gcaactcgaa
     3421 aaaccggatg tcgacctgat cgaaggcctc ccgccgacgg tcgcgatcga gcagcgggtg
     3481 acgcgcggcg gcggcaaatc gaccgtcgcg accgtcacgg aagtttacca tttcctccgt
     3541 ctcctcttcg ccaaagccgg cacgcaatac tgcccggact gcgatttgcc ggtcgaaaag
     3601 cagagcgtgg ctgcgatcgt taggcaggtg gagacggcgg cgcgccgcgg cccgctcaag
     3661 gtgctcgcgc cgctggtcaa ggcgcggaaa ggctttcaca ccgaggttgc gcgctgggcc
     3721 gagcggcagg gtttcgacac gctttatgtc gatcgcgcgc tcatcccggt cgcgcgattc
     3781 cgcaaactgg agcgcttcaa ggaacacaca atcgacgcgg tcatcggggt gattgacgcg
     3841 aagcgtatcc tgaaagcgcg caatcttgtg cagcgcgcgc tcgacatcgg ccgcggcacg
     3901 gcgcatctgc tggacgcgaa gaatcgcctc acggtgatga gcacggaaat gagttgcccg
     3961 ggttgcgggc gggcgtttga ggagctggat ccgcgcctgt tttccttcaa ctcgccgcac
     4021 ggctggtgcc aggcgtgcgc cggtttcggc gaaatctgga acgagaaact ccagcaaaac
     4081 gaggaggaga cgggcgaatc gatgctcgag aatgagctcc aggcggagcg acaattcgaa
     4141 acggtcgatg agaccgaagc ggtcgtctgt ccgcaatgcc acggctcgcg cttgaacccg
     4201 atcgcgcggc acgtgcggtt gcagggaaag acgatcgacg aattcgtcgc ccgatctgcc
     4261 ggtgaagcgc gcgggttgat cggccagttg cgttttcgcg ggacgcaaaa agtgatcgcg
     4321 gccgatctcg tgccggagat cgaacagcgc ctgcgcttca tggaaaacgt cgggcttggt
     4381 tatctcgcgc tcgggcgttc ggcgaaaact ctgagcggcg gcgaatcgca gcgcatccgg
     4441 ctcgcggcgc aactcggctc caatctccgc ggggtgctct acgtgctgga tgaaccgacc
     4501 attgggctgc acgcccgcga caacctgcgt ctgctcgaaa ctttgggcgc gctgcgggaa
     4561 aaggggaatt cgctcgtcat cgtcgagcac gatgaagaga cgatgcggcg agccgatcac
     4621 atcatcgatc tcggcccggg cgcaggagtc gatggcggcg aggtggtggc tgagggaacg
     4681 ctgcgcgaga ttgagaaaag cgcggcctcg cagaccggcc gctgtctgcg cacgccgctt
     4741 tgtcatccgg tgcgaaaatc gcgccgctcg ttaggcgaag tcgaggattg gctggagatt
     4801 cgcggcgcgc agaccaacaa cctgaagaac gtcgacgcgc gttttccggt cgggcggttc
     4861 tccgtcatca ccggcatttc aggatcgggg aaatccaccc ttatgcgttc ggtcttactc
     4921 ccagcggtgc ggcagactct ggggagcgca cgcgattcgc gtgccgtttt cggcgactcg
     4981 ccgaaaacct cgcgcggaaa aaaagttcgc gctggcgagg tcgccatcgc cggcacgcga
     5041 gtcgcgtgcg ctccccagat ctctggcgcg gacgcgctcg aggcggtcta cgaagtcgac
     5101 caatcgccca tcggcaagac ctcgcgctcg acgcccgcga cctacatcaa ggtcttcgac
     5161 gagatccgaa atctcttcgc ccagctcccg gtctcgcggg tgcgtggcta cagcgcgagc
     5221 cgcttttcct ttaacacgga aggcgggcgc tgcgaaacgt gcggcggcca gggtgtgctc
     5281 aagctggaga tgagttttct cccgagcagc tacgttccgt gtgaggactg cggcggccgg
     5341 cgttacaacc cgcagactct cgaggtgctt taccacgacc gcagcatcgg cgacgtgatg
     5401 gatatgacga tcgggcaggc cgcggaattc ttcgccgcca atccgaagat cgcgcgcccg
     5461 ctctcattgc tggtcgagac cggtctcggc tacctgaagc tcggacagcc gagtccgacg
     5521 ttaagtggcg gcgaagcgca acgcctcaag ctcgcaacga aattgacccg cggcgtcggc
     5581 cgcgcgcaaa acgaacggct gcgcaaaatg cggcagccca aatcgaccct ctatctgctc
     5641 gaggaaccga cgatcgggct gcacatggcc gacgtggagt tgctccttca tgtccttcat
     5701 cgactggtcg atgacgggaa caccgtcatc gtgatcgagc acaacctgag tgtgatcgcg
     5761 gaagccgatt acgtggtcga ccttggtccg gaggcgggtt cgttaggcgg ggaaattgtc
     5821 gcaaccggca cgccggagga ggtggcgaaa aaccgacgga gcaggacggc gccttttctg
     5881 cgcgcggttc tggaccgggg cctccttgcc gcgcgcgagc gaaaagcggc actgtagatc
     5941 cttcgctctg cgaagcgcgg gcgttgcgtt gattgcagag ggcactcagt cgcagcgccg
     6001 ccttctcccc gcgcctcgcc cagcggagcg tctacatttg ccgcccgcgc caatctcgcg
     6061 gaaactcgcg cgctcgatgc ttgcctatta tctgcacgat ttcgatccgt tcattttcca
     6121 tgtctggggc gacttcggac cacgctggta cggcatggcc tacgtggcgg ccttcgcctg
     6181 cgggttttgg ctcctgcgcg tgctcgcgca acgcggctac actcagctcc ccgccgcaca
     6241 ggtgggcgac ttcatcacct ggggcgcgct ctttggcgtg atgctcggtg gccggctcgg
     6301 ctacgttctc ttttatcggc ccgacatgct gcgcgatccg ctctccatcg tgcgggtctg
     6361 ggaaggcggg atgtcgagcc acggcggcat gctcggcctg atcgcgttca ctttttggta
     6421 cgcgtggcga cacaaactgc cgtggaccaa tctcggcgac aatctcgtcg tcgtggcgcc
     6481 gatcggttta ttcttcgggc gctgcgccaa tttcattaac ggcgaactct acggacgcgt
     6541 cacgaccgtg ccctgggcga tgttgttccc gaaggaattg ctcgaaccag agaacgcggc
     6601 ggaagcggag cgggccctgc tggcgacgca gcaggtcgac ccgcgcctaa cgactccaga
     6661 agcgctcgtt cagggcctgc acgccgagcc gcaactgcgc gcggtcctgc gcccgatcct
     6721 caccccgcgg catccgtcgc agatctatga ggcgctcctc gagggcgttt tgctcttcac
     6781 cattctctgg ctggtgcgca cgcggacccg gcagccggac ggcgtcctga ccggtctctt
     6841 cttcgtctgc tacgcgatct tccggatcgt ggtggaatat tttcgcgagc cggacgcgtc
     6901 gcttatcgcg ggctttacgc gcgggcagtt tttttccttt tttctgatcg cgatcgggct
     6961 ggctttcatc atcgccggaa aaatccgaaa tcgacgcacc gcgttgtaga cgcctccctg
     7021 tgggaggcgc ggtcttcgtg tcgcatgcag gtcaagtgga cgaagcagac cgtctccgcg
     7081 cttcgctgtg cgaagcggcg agaacagatt ttcatcaatc tggccgcttt cgtttcgcgc
     7141 gacttccgct attactaacg aaggatgacg gacctgtttt tccccggctt cctcgcggcg
     7201 ggcgggctcg ttttttcctt cgagcactcg accgtcgccg ggaagatggt gctcctcctg
     7261 ctcgccatcg ggtcgatctt cagctggtcg gtgatggtga cgaagatgcg ggtggtccag
     7321 tttgcgcgga agcaaagcga ccgttttctc gccgctttcc gtcaggaccg ccagccgttg
     7381 cgtttgtttg aatcgaatgt gcgtttcccg ggctcgccgg tcttccaggt gtatcgcgcc
     7441 ggttgtgaag aattgacctt tcacctcctc ggctcggcgg agttggacga gacatttcgc
     7501 gcccggctcg agatcgcgga caagatcacg ccggcgcaaa tgcatgccgt cacggctgcg
     7561 atggagcggg cggtgggcga gactgcgctc aagctggagt cgcagatgat cctgcttgcg
     7621 acggcggtga gcggcgcgcc gtttctggga ttgttaggca cggtctgggg cgtgatggat
     7681 accttcaccg gcgtggccga ggccggctcg gccaatctcg ccgcgatggc gccgggcgtt
     7741 tccggcgcgc tcatcaccac cgtgaccgcg ctctgcgtcg cgatcccggc gatgttcggc
     7801 tacaactttc tcgtcaccag catccgcggg atgatcgtcg agatggataa cttcgcggcc
     7861 gaactcgcct cggaatttga gcacaaatac gtcgagcacg gcggccgcgt gtcggatttt
     7921 cgaagatgag aagtggaatt gatcaggaaa acaggaaagc aggaaaggaa agagaacatc
     7981 actctttttc ctgcgttcct ggtttcctga ttcatccaaa accgaactga tgcgccgcta
     8041 ttccaaccgc agttctctca cgacgctctc cgagatcaat atcacgccgc tgctcgatct
     8101 cgcgttcgtt ctcctgatca ttttcatgat caccacgccg ctgctcgaga acagcaagag
     8161 cctgatcatt ccttcgagcg cgacgaccaa tgctccgatc aacccggcgg cggtgcagac
     8221 gatctcgatc gatcgcgatt ccatcatccg tctgaacgag gaagaaatgg acgccgcgac
     8281 tctcgagagc cggcttgtcg agttgaaacg cgcgatgccc gaggtggccg tggtcatccg
     8341 gccggaccgc gaattgccgg tgcagaaact cgtcagtctg atggacgcgc tgcaacgggc
     8401 cgacatcacc aaggtgggaa tcgcgacgca ggaagaacag aagtagcgat gactcgcgac
     8461 gcgcgtttct ggcgcaacgt gaccattgtg gcgctgatcc acgtggtcgt gttgctcggt
     8521 ttgctctggt ggagccgcac gccgacgcgc gtcccgaaag acgtcgtctg gatggaaggc
     8581 ggggccggga cgatggccgc ggcggcggaa actccggcgg cgaccccgga gtcggcgccg
     8641 gtcgagacgc cggaaccggc gcaggaagag ccgacgtccg aagtcgcgcc gaccgccaaa
     8701 agcgaaatag aaattcccgc cccggcggcg accgcgaccc cgactccaac tccaacggcc
     8761 actccgaaac ccactccgac cccaacggcc aaaccaaccc cgcgtccgac gcccaaaccc
     8821 acgccgaagc cgacgcccaa accgagcgcg acgccgaaga aaaaggcgac gccgaaacca
     8881 aaacccaagc cgaccccgga caaggaaaag gaggcagcgg ccaaggaact ggaaaagaaa
     8941 aaagcgatcg ccaaggccgc gctcgccaaa gagaaaagtg acgaaggcga agaaggatcc
     9001 ccggaaaagc cggtcaagaa aggaaccgcg ggcaagggcg gttcgggggg caaaggctcg
     9061 gacgcaggcg gcgcctcgga attcggttgg tacggcaaca tgctgcacga ccggttttac
     9121 agcgaatggg tgcagccgac ttccgtcgtg cacagcgcca cgctttccgt tcttctccgg
     9181 attcgcattc aaaaggacgg ccgcatttcc gattactcga tcgccaagtc gtccggcaat
     9241 ccggtggtgg acgattcggt gacggcggcg gcggcgcggg tgaaacgggt ggaccctttg
     9301 ccttccgggc tgggcgacga ttattatgat gtgaacatca acttcgaatt aaactagaag
     9361 atgcgcagac aacgaatttc agatttcaaa tttcagattt ccagtgtgat ggtcactgcg
     9421 ctgctttttg gcctaacgac tggctcagcg cgcgcggtgc aagacgtgcc gacgatcacg
     9481 gtgagcaagg gcgaccagat caacctcgcc gtctccgcgc tcggcggcgg cgagggagcc
     9541 gccgttacaa aggtggtgca aaacgatctc gcgctctccg gctactttac ctttggcggc
     9601 gcgaacagtt cctacaccgt gcgcgggaca gcgagcggcg gcagcctgca gggccaggtc
     9661 gtcgatcaca gcggcgggac ggtgctcgcc aaaacctacg gtggaagcac gcgcgagaac
     9721 gcgcaccaat tcgcaaacga cgtcatcgag acgctgaccg gccaaaaggg aatcgccggg
     9781 agcaagatca ccttcatcgc cacccggagc gggaagaagg aagtttatct ggccgactac
     9841 gatggcagca acgtcaggca gttgacgcac gacaacacga tcagcgtgca tccttcgctc
     9901 agcgcgaacg gccgccgcat cgcctacacc ggttaccaga gcgggtacgc ggatgtttac
     9961 gtgatcgatc tcgcgagtgg cgcgcggaat cgaatcgtga actatcccgg gacgaacagc
    10021 ggcgcgacct tctcgcccga tggcagccgg ctcgccctaa cgatcagcaa agacggaaac
    10081 ccggagcttt acaccgtgag cgcgagtggc ggcggcgcgc gccgcctgac gcacacccgc
    10141 ggggtcgagt cgggaccgac ctggtcgcct gacggggatg aaattattta ttcgtccgat
    10201 gaaggcggtt cgccgcagct ttgccggatt tcggcctccg gcggtgtggg ccgcccgatt
    10261 tccacgggcc gcggttattg caccgagccg aactggtcgc ccgacgggaa gaaagtggcg
    10321 ttcaatgtcc ggagcggagg cgagtttcaa gtcgcggtcc tcgatttgcc taacggcggc
    10381 acgcggatag tgggcgtcgg acaggacccg gtttggggcg ccgattcaag gcacctgatt
    10441 ttttcggaag gaggcgcgct ctaccttctc gacaccgtct cgagccggaa gagcaagatc
    10501 gtcgatggcc tcggcaaaat caccgagccg agctggtcgc ggtaggattg ccgccgctag
    10561 agcgcgaggg atttcccccg aaatgcatcc cgcggcgatc ctgttcatcc cgtaaatcct
    10621 gtctaagcta gcgccgtgaa agcgggcttt ctcgaaaagc tgatcgggcg cctcggccgc
    10681 atcgggcccg aagatgtgca gaattacctg ctccggctcg ccgaggagaa aggttttctc
    10741 gaaacgattt tcaacgccat ccaggaaggg atcatcgtga ccgattcgaa cggccggatc
    10801 acctacctga atcacgcggc cggcgaaatc ttcggactcg acggcgagaa cgcgatcggc
    10861 aaaccgctcg ccgagcaggt gcgcgggctc gattggcagg cgctctctca ctccgacacg
    10921 gtcctgagcc gcgatatgga gattttttac ccagccaatc gtttcatcaa tttctacgtc
    10981 gtgcccttga tgattgagcg gcgttcgcct aacgaggagc cggacgaact ggcggaagcg
    11041 gtcggtcacg ccatgatcct gcgcgacatt accgagagcc ggcgctcgac ccagcagacg
    11101 atcgagtccg agcgcttgtc ggcgctgacc ctgctggccg cgggcgtcgc gcacgagatc
    11161 ggtaacccgc ttaactcgct ccatatccat ctccaattga tggagcgaaa agtgaaaaaa
    11221 cttagcggag cggaggacaa gcgcgcgctg caggaatcga tcgatatcgc acgggccgag
    11281 atcaaccggc tcgattcaat cgtgacccag tttctccgcg ccgtccggcc gtcgaaaccg
    11341 caactccatc ccgaaaacgt gaacaccatt gtgcaggagg cggtgcgctt tttccgcccc
    11401 gagatcaagg accgcgacat cgtggtcgaa accgaattgc gctccgatct gccgccgctg
    11461 gaactcgatc gcgaccagat gaagcaggct ttttacaacg cgatcaaaaa cagtttcgag
    11521 gcaatgaagc ggcacggcat cctgcgcatt cgaaccgatc gagatgacac gcacgtgaag
    11581 gtcagtttta ccgacacggg cggcggaatc tcggcggaga atttgagccg ggttttcgat
    11641 ccctacttta cgaccaagag cggcggctct ggtctcggat tgctgatcgt taggcggatt
    11701 gtgcgggaac acggcgggga gctggcgatc gagagcgcgg aaggcaaagg gctgacgctg
    11761 acgattcgtt tgccgttctt ggatcgaaga gtgcggatgc tgccggacag ggaaaagatg
    11821 aaggatgaag gatgaaggcg gaaaaccgga gccgcgggcc gcgtgcgcct ggcccgctgg
    11881 tcttggcgcc tcgccaaaac aatctttttg gcaagcggtt gagttcttac agcgcgagcg
    11941 gtggccacgt ttcggaagag ttcgcgaccg cgagggcacc gtcgtcagcg tgcgaggcgc
    12001 acgcgctccc cggacaacgc cgaactgaaa gcactgtcca tcggcccgtc cgcttttctt
    12061 ttcatccttc cgccttcatc ttttcttccc aatgcaaccg acggttctga tcgtggacga
    12121 cgaaaagcac acccgtgatg ggttgcggag tttgttaggc gagcactacg acacctatgt
    12181 cgcggccgat gtcgcgggcg cgctcgacgt tttggaacgc gaccagattg acctgctcct
    12241 gaccgacctt cgcctcggcg ccgaggatgg catgaccttg atcgagcgcg cgctcaaact
    12301 gccgcatccg ccggtttgca ttatgatgac ggcttacggt tcggtcgata ccgcggtcga
    12361 ggcgatgaaa cgcggcgcct acgattttgt gaccaagcca ctcaatctcg acaaggtgga
    12421 gatgttgatt gcccgcgcgc tcggcagccg ccggctcgag caggaaaacc gctcgcttcg
    12481 gcaggaggtg gacgaacgtt tcggactgga gaatctaatc ggggaatcgg tcgcgctgcg
    12541 cgaagtgctc gacacgattc gccaggtcgc gccctcctcg gccaacatcc tgctcgaagg
    12601 cgagagcggc acgggcaagg aactggccgc gcacgcgatt cataatctga gccggcggca
    12661 caaggggaaa tttgtcacgg tgcattgcgc cgcgctttcg ccgcagctcc tcgagagcga
    12721 gcttttcggg cacgagcgcg gggccttcac cggcgcgcac gaacgccgga tcggacgttt
    12781 cgagcaggcg aatggcggca cgattttcct cgatgagatc ggtgagatcg acgcgaccac
    12841 gcagatcaaa ctcctgcgcg tgatgagcga agagcgagcc ttcgagcggg tcggtggatc
    12901 gcaaactttg cacgccgatg tgcgcctgat cgccgcgacg aacaagaatc tcgagacgct
    12961 ggtgagcgag ggaaaattcc gcgacgacct ctattttcgg ctcaacgtgg tcaagatcac
    13021 gatgccgccg ttgcgcgagc gaaaggaaga tattccgctt ctcgtgcgct ccttcctgcg
    13081 ccatttctgc aaggcgaatg agaaaccgct cctcgacctg acaaccgatg cgatgaacgc
    13141 cctgctggct tacgattggc cgggcaacgt gcgcgaattg cggaccgcaa tcgagcacgg
    13201 cgtggtgatg gcgaatgggg cgaaggtcac gctgcgcgat ctcccggccg ccgtgcgcca
    13261 ggccgcgccg gcggcgggag tgcgcgggca cgctttcgac gagaaggcga gcgcgtttga
    13321 cttgcacgcg acggaaaaga ggctcattac gcaggcgctc gcgacgacca ggggaaacat
    13381 caccgccgcg gcgaaaaagc tgggcatcag ccgccgcacc ttgcaccgca agatcaacga
    13441 aatgaacgcc gctcagccta acgaccacca cgagtccgga aagatggacg cttcttccga
    13501 cgatgcccgg gagtgacgac gcggtgcagc gcgcggtcca tgcgctggaa accggtggca
    13561 tggcggtctg gatccgccgc gcgctggcct tcctcctgat cgttgggctg gccctctttt
    13621 atcttctcca cgagttccgc ggcctggcca cttcgcaggc gatggatcag gcgcaaatcg
    13681 gccgggagct gctccacggt cacggctgga aaacgaagtt tgcgcgtccg ctcgccgcgg
    13741 gccagctgca gcgccggggc aaggacgtgg ccgggaaaat atggaccgac acctataacg
    13801 cgccgctccc gccgctcgtc gatgcgattg cgctccttcc ggtccgatct catctcaaga
    13861 tgacgacgcg cgacatcgtc tatctcggcg acaaaatgat cgcactcctg tcgatggtgt
    13921 tctttctggc ctcggtcgtg gttctgtttt tgatcgcatg ccggcttttt gaccaatatc
    13981 tcgccgtgct cggctgcgcc ctggtgcttc tcggcgacac cttctggcaa tactcgctct
    14041 cgggcctgcc gcagatgttg ctcctcttac ttttcaacct cacgctctat gtgctggtgc
    14101 gggcaatcga agcgcgggcg gagggtcggg ctgtgctgcg cttactcggg ttggccgggg
    14161 cgggctttgg cctgctcgcc ctctcgcatg ccctcaccat ctggatcttc ctcccgacgc
    14221 tgatctttgc cgcgctgtat tttcggccgc gcctttgggc ggcgatctgg atgctggcgc
    14281 ctttcctcct cctctacacg ccgtggctgc ttcgcaatta tctcgtctgc ggcaacccgg
    14341 ccggcgtcgc gcttttttcc tttttcgacc agatcgggat gagcgaagcc ggccacatgc
    14401 gccatcttgc gatcgactat caggggatga gcatcggcgt tttgcgcgat aagataagcg
    14461 acaatgtggc cgggcaggcc agccggattt tgcaatattt cggctggagc gtggtggcgc
    14521 ttttcttttt cgcggcgctc ctccatcgtt ttcgccgtgc ggtgacggcc acgacgcgct
    14581 ggttcgtctt cgtgatgtgg ggcggtgcgg ttgttgggat ggcggtttac ggcgtcaacg
    14641 aggaacaggg cgtcgccgcc aaccagctcc atctgctttt tgtgccgatc atgacttgct
    14701 tcggccttgc ctatctcctc gtgctctgga accgcctcca gatcacgagc cgcctcgcgc
    14761 gttttgcctt cctgaccctc ttttttgtct tgtgcggagg gccgatgatt ttcaacctgc
    14821 ttttcgcgag cgccaagacc ggcgtccgct ggccgcccta tgtggcgcct tacattgccg
    14881 tgatcaacga ttggatgaag cccgaggaaa tcaccgcgac cgacatgcca tgggcggtgg
    14941 cgtggtatgc cgatcggcgg gcggtctggc tgccggacac gattcgcgat tttacggaca
    15001 tgagtgatta cgatctgctc ggcggcaaga tcaacgggct ctacctgaca ccaatcagcg
    15061 gcagccaaaa caccctgaag aacatcgtgg agggcgaata caaaggttgg gcgccggtca
    15121 tcttacggag cgtcgacctg aagaaatttc cattgccctg ggcgacgctt cttccgctcg
    15181 atgacgagtg catctttttc agcgatcatg accggcggaa agccagcacc gaccaaccgt
    15241 gatggcggcg gcgaagaaaa ttttcgggac ggacggcgtg cgcggggtgg cgaacgtcga
    15301 gccggtcacg gccgagaccg cgctcaagct cgggcgcgcc gcggcgcatg tctttagcgc
    15361 gctcaatccc caggcgcacc cgtccggctt gcggccgaag atcgtcctcg gcaaggacac
    15421 ccgactttcc ggctacatgc tggagaatgc gctcgttgcc ggcatcacgt cgttaggcgt
    15481 agacgtgctc ctgatcggtc cactccccac gcccggggtg gcctacatca cgcgctccct
    15541 ccgggccgac gccggcatcg tcctctctgc ctcgcataat tcctacgagg acaacgggat
    15601 caaatttttc cggcacgacg gctacaaact ggatgacgca atcgagcgga agatcgagca
    15661 gctcgtcttc accggcgaga tcgattccgt gcgaccgaca gcgaaaaaaa tcggccgcgc
    15721 gacgcggatc gatgacgcgc tgggtcgtta cgtggagttc gccaaggcga gtttcccgcg
    15781 cggcatgtcg ctcgagaaga tgcgcatcgc ggtcgatgtc gcgaatggcg ccggctacaa
    15841 atcgaccccc tgcattttgg gtgagctggg ggcggaatta agtatcgcgc acaatcagcc
    15901 taacggaacc aacatcaacg cccattgcgg cagcgtctac ccgcaggaaa tccagcgact
    15961 ggtgagggaa agcggcgcga cggtcgggat cgcaaacgac ggcgacgccg atcgcgtgct
    16021 gctgtgcgat gagcggggcg aggtcgtgga tggcgatgaa attctggcca tggcctcggt
    16081 cgacctgctg cggaacggtc agttgcagga aaacactctc gtcgccaccg tgatgagtaa
    16141 cttcggtctc gacgaagcgc tcgccgcgca tggcgggaaa gtggtgcgca cgaaagtcgg
    16201 cgaccgttac gtgatcgagg aaatggtgcg gcaaaacctg aacctcggcg gtgagcagag
    16261 cgggcacatc atctttcgcg atttcaccac caccggcgat ggcatcatca gcgcgctgca
    16321 aatcctgcgg atcatgcagg cgacgggcaa accgctgagc gagctgaaaa aatgcctgac
    16381 gaagtatccg caggcgcaac gcaacctgct ggtcagggag aagccgccgc tcgagacgtt
    16441 gccggaagtg atgaagctgg tgcaggacac cgagaaggag ctgcgcggca acggccgcgt
    16501 gctgctgcgt tattcgggca ccgaggcgaa aatccggctg ctcatcgaag gccgcgagcg
    16561 gacccagatc gatcatcagg cggatcggat tgcgggtgcg attcagagcg cgatcggact
    16621 gtagccgctt cgctctgcga agcgcgggcc ttggcttgga ttgcggaaaa cgctccaaaa
    16681 gtcatggcgc ttaggcgacg gcgattttct cccgcgcttc gccccgcgaa gcgtctacag
    16741 ttgcgtgctg cgttacggcc tgctttcctg attcgccgcc atgaaccttc cgcaagtcat
    16801 cgaagcgctc ctcttcagcg cacaaagacc gctcagcacg cgcgagttgg tggccgcgat
    16861 ccggggcgcg ggcgcagcag acgaatttac gcctaacgag tttgcgaaaa ccaccgaagc
    16921 cgccgtggcc gcggcgctcg agcaactgaa gatcgaatac ctcgagcagc agcgcgcctt
    16981 tcaactcgtc gaaaaggcgg aaggctggca actggcgagc gacccgcaat ttgcccgctg
    17041 ggtgcgccaa ctttttcccg ccgcgaagcc ggcccgcctc accccacccg ggctcgagac
    17101 gctcgccatc atcgcctacc gccagccgat cacccgcgcc gacgtagaag ccgtccgcgg
    17161 agtcgcaatc gaaagcgtgc tgcaaactct gatggagcgc gggctggtca ggattgccgg
    17221 ccgcgccgag gtcccgggcc ggccgttgct ctacgagacg acccaatttt ttcttgagca
    17281 ctttggtctg cgcaatctcg atgagctgcc aaactcggag gaactgaagc ggcgcgagtt
    17341 gccgagggcg ccgatggctc ccgcggaaca cggcgccgct gcggagtccg ccccagagca
    17401 aggcgaaaag atcgcgagcg agccggccta gcggcagacc ggagcgtgcg tgaaggttct
    17461 cgttcgcgcg tgacatttag gtcgatccgg cgattcttaa cgcacctgaa tttatccaat
    17521 gaagacattt gcttttggtt gcggtggaat tttgctcgtg ctcgttctcg tggcggtggt
    17581 ggtcgggatc gcggtcgccg gcaaatacaa tgggctggtc aagctcgacc agagcgtcaa
    17641 tcaatcctgg gcccaggtgc agaacgttta tcaacggcgc gccgatctca ttccgaatct
    17701 cgtccagacc gtggccggcg ctgccgactt cgaaaaatcg acgctcatcg ccgtcacgaa
    17761 cgcgcgtgcc agtgtgggtc aggtgaaact cgatccgaac aaggcgccga ccgatgccgc
    17821 gcagctcgag cagttccaag ccgcccaggg ccagttgagc aacgcgcttt cgcgccttct
    17881 cgtggtgagc gaaagatatc cggagttgcg cgccaaccag aacttccttg ccctccaggc
    17941 gcagctcgag ggaaccgaaa accgcatctc cgtggagcgg gggaatttca atacaaccgt
    18001 ccaagcctat aacacggcgg tccgcacttt tccgacgaac cttttcgcag ggatgttggg
    18061 cttccagccg cggcccttct tcaacgcaca acccggcgcc gagaaagctc ccgaggtaaa
    18121 attcaacttt ggcaacacgg ctcccgcgcc ggccgccaca gctgcggcga cgccataatt
    18181 gctggcagcc cgagccccga aaacattcgg ggtcgaggga taccgtgaat acaaactgca
    18241 aggttttgac tgcgcccgga atggcatgaa gacgcgcctg cttcccggct tcttcgcgct
    18301 tttcgtttgc gcgaccggcc cgctggcggc ggaaaggatg ccgtccaaac cggatcgcta
    18361 cttcaacgat tacgcggcgg cggtcgatcc ggcgaccgcc gcgcaattga acgaacaact
    18421 ggcgcagttc gagcgcgaga cttcgacgca aatcctggtc gcggtttacc gatcgatgga
    18481 aagcgagtcg tcggtcgccg attataccca gcgcatcgcg caaacctggg gcgtcggcca
    18541 gaaaggtcgc agcaacggtg cggtcctctt cgtcttcgtt gacgaccacc agatgttcat
    18601 ccagaccggt tacggcctgg aaggagcgct gcccgacgcg acttgtttcg acatcacgca
    18661 caacctcatc gcgccgtttt ttaagaaagg ggattatgcg ggcggtttga aagctggggt
    18721 cgcggcgatg atgcaagcgg tcaaggggga atacaaaggc actggccgca cccaaaaaga
    18781 atcgaagacc ggtggctcgt ccggtatgcc agtcgggctg attgttttcc tgatcattgt
    18841 ggccttcgtc attttcgcca atcggtcccg aagccgcggc tacggctaca gcggcttcgg
    18901 cgggccgttc atcggtggct tcggcggcgg cggctggtcg ggtggtggcg gctccggcgg
    18961 tggcggcttc agcggcttca gtggtggcgg cggcagtttc ggcggtggtg gcgccggctc
    19021 cagttggtag gcccatgcgc acgaaacatt ttctcaataa gatcgagcac gctcgcatcg
    19081 tccaggcgat cgccgcggcg gaaaagaaga cctcgggcga gatccgtgtt ttcattcagc
    19141 acggggcaat ggacgacgcg gtcgcggcgg cgcgcgcaaa atttcaaaag ctcggcatga
    19201 ccgcgacacg cgaacgcaat ggcgtgctca tcttcgtcgc gccgcgtgcg caaaaatttg
    19261 cggtcatcgg cgatgaaggc gttcaccagc gttgcggcga cgtcttctgg cagcaactgg
    19321 tggcctcgat gcagacgcat ttcaaagcgg aaaactttac cgacgcggtc gtgcacgcga
    19381 tcgagcggac cggcgcactc ctcgcggaac attttccgcg cgagccgggc aaccacgacg
    19441 aattgccgaa cgcggtcgag gaaggcctgt agccgctgcc ccctggttgg cagcgcgagc
    19501 gtcagcgtca ctcactcccg aagctgcgaa cacccgtaat cccgtgcttc ccacagggaa
    19561 gcggctagag gcggcgtcgc acttgcaaag ccggcgcgat ggtgttcatt ccccgtcctg
    19621 cagcgagttt tttaaacgtt gcgccgttgg tccctgggtt tccgtcgctg gacgcgagac
    19681 cacgctcgcc aggggaaacc cggcgctttt ggaaggaaga agtaagaagg cagaaggatg
    19741 aagtgccgtc aggcgccaac ttttcttaat tcataattct tccttcatcc cttacaccct
    19801 atgtcagttc gttacggtcg ttttgaaatg cccaagaccc tcgtcaagga cgaggagtca
    19861 tccactgaga cgtacgccaa atttgtcgcc gaacccttcg aggccggcta cggacacacc
    19921 gtcggcaatt cgctccgccg cgtcctcctt tcctcgctcg aaggcgccgc catcaccgcg
    19981 gtgaaaatca ccggcgcaca gcacgaattc ggcacgctcc ccgcggtcgt cgaggacgtg
    20041 accgacatcg tcctcaattt gaagcgggtg aagtttaaag cctccgatca cgaggcgcgc
    20101 aagctctcgc tcaacgtgaa caaggaaggc accgtgaccg ccggcgacat ccagctgacg
    20161 cagggttacg aagtgctcaa cccaaagcag gtcatctgca cgctcgacaa gaaacacaaa
    20221 ttcgaagctg agctcgatgt ccgtgtcggc cgcggtttcg cgaccgggga cgaaaacaag
    20281 cgccccgacc aggccattgg cctgatcccg atcgactcca tcttttcccc ggtgacccgg
    20341 gtgaaatacg cggtcgaaaa cacccgtgtc ggccagcgca ccgactacga caaactcatc
    20401 ctcgaagtct ggactgacgg gcgcctcacg ccggacgacg cgctcctcca ggcctccgcc
    20461 atcctgcgcc atcacctcga tgtcttcgtc aatttcaacg aggacgtgat cgaattcgac
    20521 gaaacgccag cgggcgtgag cgaagaaaac gtcaagctga agaagctgct caacatgagc
    20581 gtgaacgaga tcgagctgag cgtgcgcgcg gccaattgtc tgaacaacgc gaacatcacg
    20641 accgtcggcc agctcgcgca aaagacggag gcggagatgc tgaagtatcg caacttcggc
    20701 aaaaagtcgc tcaacgaaat caaggacaag ctgcagcaac tcgggctcgg gctggggcag
    20761 aagttcgatc cgggtttggt tgaaatcccg ctgccggccg acggggcgaa cggcgcggac
    20821 gaggaatagt cgttaggcaa agcattcgcc ggagaagcag ttatgaggca ccaaaagaag
    20881 accgttaagc tgggacttac cgcggaacac cggaaggcgt tgctggcgaa tcaggtttgc
    20941 agtttgatcg agcaccaacg gatcaagacg accctcgcaa aggcgaaagc ggtccgtccg
    21001 ctggcggaaa aaatggtgac gttaggcaaa aatggctcgc tccacgcgcg ccggacggcg
    21061 ctcgccgttc tgcggcagaa agacgcggtc aaaaaattgt tcgacgagat cgcgccgcgc
    21121 agcgcgagcc gccagggcgg ctacacccgc atcatcaagc ttggccagcg caagagcgac
    21181 tcggcggccg ttgcttttct cgagtgggtg gatgcagcgg aagtggtgct cgaggaaacg
    21241 acgccggcgc aaaaaggcag gaagcgcaaa gcggcgaaag gcgaagcaag tcccgaaggg
    21301 gacgccaagc cgtcgggcaa ggcgaagaaa accgagccta acgaaaagga aacgggcgcg
    21361 aaagagtagc gcgtctgggg cgtcccccgg ctcgagagag tctcgcccca ccatcgtccc
    21421 gtttttggca ctgcctcatc ccgtcggcga cacactcgcg gtcgcgttgg gttcttgggt
    21481 cgtcgcacaa gcatctttct gtcagcgatt tacactttcg cggatcactt ggcataccgc
    21541 ttgcatgaga ctctggggtt aagaaaggac acccatgaca cacctaccca acgccaaacc
    21601 cgtgcacgaa gaaagcacct accaactgct catccagtcg gaggaaaaag agcgcggcct
    21661 gatggaagcg atcgtttacc tgctcctcgt catcgccacc acgacgacga tctggcagtt
    21721 tagccacgtg ccggtgacgt ttgccgacct cggggcgagc acccagaatt cgattaccgc
    21781 cgcgctttga ggggcggacc tgtggtcttc cgcccgtcat cgccgttctg tccggctcgc
    21841 aagagctcgc tgatacaatt gcgcgactgg actcgttgga agacttcacg ccgcgtcagc
    21901 caggggcatt agttgcggct tgtcagaagg aggaacgcgc tttccgtccg tcctgtccta
    21961 acttcgggac cagagagaag atcgacgcgc tgggtgttgc ctaacgaagg cgcgtcaggt
    22021 gccagattaa ctttccgtcc gcctcgatct cgagggtcgc gacgctgggc accattccgc
    22081 ggtccgggcg cgtgacgcag ccgggattaa gaaagagcac gcctccccgg cgttcgttgc
    22141 gcggcatgtg ggtatggcca tgcagcagga catccacgtt ctccggaggt tgtgatggtg
    22201 gaatgtggac gagacggaaa cgaaggtcac cgcgttgcag gtcgaccacc agtggccact
    22261 cgaaattgct gtcgcagttg ccgcgtacca gggttaccgg cggcccaaga ttttccagct
    22321 cgacgagcag gctgggcgcg cagagatcgc cgagatgcca gatttcatct gcgccctctg
    22381 ccagcgccgc aagattttcc gggagatgat tgtgggtgtc ggcgaggaca aaaatccgca
    22441 ctgctttcgg ccccatgcgc tcgttatcct gagcgaaacc aaggatcgcg caagacagaa
    22501 ctgacgttgc ttcccagcct aaccgatcta ctgttcaggc ctgtgtcttt caacaacttc
    22561 gggctttccc cggaagtcgt gcggggaaca caggcgatgg gcttttccga gccgacgccg
    22621 atccagctcc gcgcatttcc aatcattctt tccggcaaag atctaatcgg taccgcgcaa
    22681 accggcaccg gcaagacggc cgctttcgca ttgccgatca tgactttgct cgcccaccac
    22741 ggcacctttc gctgtctcgt tctcgagccg acgcgcgagc tcgccgcgca ggttgaaacg
    22801 gcttttcgtg attacgggcg cttcactgac ctgcgcgtct cggtcgtgca tgggggggtc
    22861 ggctacggca aacaaaagga ggaagctgcg gctggcactg acgtcgtctg cgcgaccccc
    22921 ggccgcttgc tcgatctgct cgaacaacgc gcgatgaatt tccgcaacgt caacatcctc
    22981 gtgctggacg aggtcgatcg catgctcgac atgggttttc tgccggacgt taggcggatc
    23041 gtggaacaaa tttccaccga ccggcagacg ctcctttttt ccgcgaccct gccgccggag
    23101 atcgagcggc tcgccgcctg ggttctgcgg gaaccggaac tggtggagat cggcgcgcgg
    23161 cgtgccccgg ccgaaaccgt tacgcacgcg gtctatccgg tcgcggcgga gcagaagttc
    23221 gatctgctca tcgcgttgct cgagcgcacc aattacgaca gcgcgctgat tttctcgcgc
    23281 acgaaacacg gtgcggaccg catcgcgtcg cagttgaaac aggcgcggca ttcggtcgcg
    23341 gtcttgcact cgaatcgcac tcaacgggaa cgcgtcgaag cgctcgaagg ctttaagtcc
    23401 ggcaaatacg aagtcatggt cgcgaccgat atcgcggcgc gcgggatcga catcgcgggg
    23461 gtgagccacg tgatcaatta cgacgtgccg gagcatccgg aggattacgt gcatcgcatc
    23521 ggccggaccg gccgcgcgca gaacgtaggc gacgccttta ccctgatgaa cggcggggaa
    23581 ttgccgtcat tgcaggcgat tgagcgcttt atcgggcaaa aaatcccgcg actgaagctc
    23641 gagggtttcc cctacattta caccgtgctc ttcgagccgg aatcaggcgc cggcggcaaa
    23701 cgtgccatcg gcggcgggcg gacgcatcgc ggctactcct tcggccggcg ccgtcgttag
    23761 gccagcgcag cgggttccgt gcgcgctgta gatgtcgccc tgcgggcgac acgggtctgc
    23821 gttcagtccc cgggtttggt tttcgagcga ccgccagcca cttccgcgct tcgcagggcg
    23881 agccgtctac aaggcaaacc cctattcggt gaaaagctca atcgcatcga agagcgattc
    23941 cgcgaccagt tcctcggcat ccactcaata ccgtccacag gagctgcatt gactccgccc
    24001 cacgcgatgc gcgacactcg ccgcgatgga gtgccccttc aatcacgagc agccgctgca
    24061 atactacggc gactatctcg cgatcgatcg gcttctcgcc ctgcaaaaac cgctcagcct
    24121 gcaggagggc aagctgctcg cgcacgatga gatgct
//
LOCUS       DATMNS010000216         7892 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3020323, whole genome shotgun sequence.
ACCESSION   DATMNS010000216 DATMNS010000000
VERSION     DATMNS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3020323"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..1248
                     /locus_tag="VNW28_07545"
     CDS             178..1248
                     /locus_tag="VNW28_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09821.1"
                     /translation="MFFRPVALALLLGPVAAAHAQQALPPEGAQVLRSSQSAQAAPDS
                     AWLDLRQNNVAHSKVQVAPPWVESVSFTPGKASNDPPTKSVFRIRLTRPDEDCQLLLF
                     RLYFDDQPEAQPEIIAWDESGTQLLRSGSLGQKTGLATSSTTIVPMIGVTSIDVEVAG
                     DGRTVRGAYLDWMKTSQVMRPLHAEQAKLMADPFGGPAPLQTGAEDAEVFGAVTAPLA
                     AETIPMGSSMQDGAAFQFGLEAQPLVALLTFEVASPQIEVPPEIYVNGEDVGPASLVL
                     PDLADPAYRGLSLSLLQTMQFQYTGWVRAQKVVPASILKAGTNDVIVIAGAGTSASAI
                     REMQIQLKYLWDKSDYLLEPLK"
     gene            1267..1677
                     /gene="gspG"
                     /locus_tag="VNW28_07550"
     CDS             1267..1677
                     /gene="gspG"
                     /locus_tag="VNW28_07550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HXA09822.1"
                     /translation="MRKNQSAFTLLEIMIVVAIIGLLLSAAIYKMAPALDVAKGTRVR
                     ADIQSIRTSLLTYSSMNGFYPTTEQGLRALVVRPDSDPQPASWRPLMDDVPKDPWGLE
                     YVYRCPGQKNPSGYDLFSAGPDRKPDTGDDDWGH"
     gene            complement(1750..2391)
                     /locus_tag="VNW28_07555"
     CDS             complement(1750..2391)
                     /locus_tag="VNW28_07555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09823.1"
                     /translation="MKTKNLALLLTWPLLCLTTSARADSDLPTTEKFARYEPMLKRSP
                     FAVATAGAPLASAPGFAKDLYVANAAHSTDGDFVTLASTTDKNLKEYLSTRETVDGYS
                     ISNIQWSTRVGETKVTISKDGQFATLTFNEALLSQSIQNPVAQIPPPPPQPMPARPAP
                     NVPMPVQRAMPVPQIPTPPPHTRPVIRRKTASQQSLRQPLRQPADVDSDFENP"
     gene            complement(2391..2939)
                     /locus_tag="VNW28_07560"
     CDS             complement(2391..2939)
                     /locus_tag="VNW28_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09824.1"
                     /translation="MIARWLAQMNQRERMLALTVGAILFLLINLAIWSALLGMSAGAR
                     SDFAAQRAARAEQKVYLEAEKTWKRRSEWLKKSQPTLTNPAEASSLLTQVKQIAGKYN
                     IQIDDPQIGAVETTPSHQSVSATFETKSGWVPLVHFFYDAERPEAFIVFETVNLMVDS
                     GDPTVMRGRFKIAKWFAPAGQK"
     gene            complement(2936..4081)
                     /locus_tag="VNW28_07565"
     CDS             complement(2936..4081)
                     /locus_tag="VNW28_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09825.1"
                     /translation="MHHVADLNEAAGLLNGGDDIVFALPVNAVLAQRLRLPTVDPAEF
                     DAMVRLQVEKAFPYPPEEVTSDFEIIEQNESDSVISSVAVHNPRLAELAGPLLSRGYI
                     PRQVTVYAAQRAASHAARGRALIIYREGEKLVSAISENGKLSLIRTVDAAEPKRLETD
                     LPQLALSAELQGIDTAFPTVLLDENCLPLRDTIERVFALRPDLMRTEAPPATVKLNLA
                     PESWRRQRAAAARQREWRRRLLWAGGAYAAAFLLFALYVVIIRLQVGHLDKVVRRDAP
                     RVEFVKNTQAAWKALSPAIDPHYYPIEVLLHLFESLPNPEVRITVYDQSARNVSVQGE
                     APSAALAYQFAEKVKKHPDLQDFTFSLETPRILPNDHAQFHLEGKTK"
     gene            complement(4173..5078)
                     /locus_tag="VNW28_07570"
     CDS             complement(4173..5078)
                     /locus_tag="VNW28_07570"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09826.1"
                     /translation="MKQGPFASRSGAALLLALWALFLLSAVLIAWALDIDSRIILAGN
                     SSRILEAEAMAASGAEVALDPSVDAGSTVLHGSPAPNQTYQARITGEGGRLNLNWILS
                     GENPQRLAILQKYLENKGIDINERERMMDCLLDWVDPDDIPRLNGAEADQDYHPRNAL
                     LQSVEELKQVKGWAEFTARPDWEADFTLYSSGPIDLAWASREVLLSLPGFNEQIVDRF
                     LEYRRGPDGIDGTEDDPVFESLEQVRLALGFTPEQFAQLSGLIGFKDPVVRVISVGKS
                     GNATRTIQMIIRKSGSPQVIRWKEL"
     gene            complement(5082..5708)
                     /locus_tag="VNW28_07575"
     CDS             complement(5082..5708)
                     /locus_tag="VNW28_07575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXA09827.1"
                     /translation="MSTVRVDQNSRRAFTLLEVTLAVVILGMMALAIYRFVSANLVAM
                     QASAALNEDNAQYAGLANLLAEELQDLPPGQGTLSGEPFKFEARSRDEMTWICSAGPG
                     LLTRYASGEYSVTLRLRPMPKGNRMQLGIVRESQDDGDSSGENQSWIPLIANLNSIEI
                     RYFDSRLNAWVDRWTDNTALPCLVRLTIDRAGSTVPWEAVLPLRRTPL"
     gene            complement(5689..6087)
                     /locus_tag="VNW28_07580"
     CDS             complement(5689..6087)
                     /locus_tag="VNW28_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09828.1"
                     /translation="MRRNKGLPGFALYEVLLGLTIFVVGVLALGRSVENCLRASSLSE
                     EENRVRQLLANRMAEVQASPVHPDAEKETKVDTGYGVVRLIQKTVPEQLEDADNTIID
                     GISRVTLSAEWSRGGVKQSKRLVFYVYRAG"
     gene            complement(6074..6547)
                     /locus_tag="VNW28_07585"
     CDS             complement(6074..6547)
                     /locus_tag="VNW28_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09829.1"
                     /translation="MPRRMGFTLIEITISVFILLLLLLLALPSLSGVLADRRLKTSLD
                     AMNQIVREAQKRSVEEHRPYLIEWQKKTVILRPESIAKGDSAAPAATLVLDKGHRFQL
                     RLPAALEKDPLAQWIFWPSGTCEPANVSFQGPNGSWEVNYSPLTARPEIVRYATK"
     gene            6712..7728
                     /locus_tag="VNW28_07590"
     CDS             6712..7728
                     /locus_tag="VNW28_07590"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HXA09830.1"
                     /translation="MISPAAQPVAPLITAAAPALRLRPMHKILLVLAALGVLGAMTWQ
                     GLATGGSPDPTRANTSPAVAVLDIGVLVFREGLECILVLAAITASMTGPQRDCRRPVA
                     WGAALAFLATLVTWMIAVRIMSALADNVSALNLQAATGLLAIVVLLVIMNWFFHKIYW
                     GGWIRAHNRRRKSLLTSGAATNSSQTRLIWGLLLLGFTSLYREGFEVVLFLQTYYLRL
                     GGGVVLKGALLGLALSGVVAILTFVLQQRLPYRNMLVTTGVLLGGVLLVMVGEQAQEM
                     QLAHWISTTPVPSLVSRVPDWAGLWFALFPTYETLIAQAIAALVVLGSYYAARRVRCT
                     AEPS"
BASE COUNT         1455 a         2507 c         2347 g         1583 t
ORIGIN      
        1 gtctacgaac gcgaactgga tcgcaccgtg gatgtgattt cggcgctcat cccgcccgtg
       61 attatcgtcc tgatcgcgat tgtcgtcggc ttcgtggttt acagtattct ctccgccgtc
      121 tttgagatga cgcacagttt acaggtccgg ccgcaatagc ctaacctcga agccaagatg
      181 ttttttcgtc ccgtcgcgct cgctctcctg ctcggtccgg ttgccgcggc tcacgcccaa
      241 caagccttgc cgccagaagg cgcgcaagtg ctccgctcga gccaatcggc gcaggccgcg
      301 cccgacagcg catggctcga tctgcgtcag aataacgtgg cgcactcgaa ggtgcaggtg
      361 gcgccgccct gggtggaatc ggtttcgttt acgcccggca aggcgtcgaa cgatccgccc
      421 acgaaaagcg tgttccgcat tcgcctaacg aggccggacg aagactgtca gctgcttctc
      481 ttccgtctct atttcgatga ccagcccgag gcgcaacccg agatcattgc ctgggacgaa
      541 tccggcaccc aattgctccg ctccggttcg ttaggccaga agaccggcct cgcgacttcc
      601 tccaccacca tcgtcccgat gatcggcgtg acgtcgatcg acgtcgaggt ggcgggcgat
      661 ggccgcaccg tccgcggcgc ttatctcgat tggatgaaaa ccagccaggt gatgcgcccg
      721 ctgcatgccg agcaagcgaa gctgatggcg gatccgtttg gcggcccggc gcccctccag
      781 acgggcgcag aagacgcgga ggtttttgga gccgtgaccg cgccgctggc cgccgagacg
      841 atcccgatgg gatcgagcat gcaagacggc gcggcctttc agttcggcct cgaagcgcag
      901 ccgctcgtcg cgctgctcac tttcgaagtc gcaagcccgc agatcgaagt gcccccggaa
      961 atctacgtga atggcgagga tgtcggtccg gcctcgctcg tcctgcccga cctggctgat
     1021 cccgcttatc gcggcctttc cctttcgctc ctgcaaacga tgcaattcca atataccggc
     1081 tgggtccggg cacagaaggt cgtgcccgcc tcgatcttga aggccggcac gaatgatgtg
     1141 atcgttatcg caggcgccgg aacttctgcc tcggcgatca gggaaatgca gattcagttg
     1201 aaatacctct gggataaatc ggattacctg ctcgagccgc tgaagtagcc ccgtcgccaa
     1261 ttatttatgc gcaagaatca aagtgctttc acacttctcg agatcatgat cgtggtcgcg
     1321 atcatcgggc tcctgctcag tgccgcgatc tacaagatgg ctcccgctct ggatgtcgcc
     1381 aagggcaccc gggtgcgcgc tgatatccaa agcattcgaa ccagccttct cacctactcc
     1441 agtatgaacg gcttttatcc cacgacggaa cagggactgc gcgccctggt cgtccgaccg
     1501 gatagcgatc ctcaacccgc gtcctggaga cctctgatgg atgacgtgcc gaaggatccc
     1561 tggggcttgg aatacgttta tcggtgtccc gggcaaaaaa atcccagcgg ctacgatctc
     1621 ttctccgccg ggcccgatcg caagcccgac acgggggatg acgattgggg ccattgacgg
     1681 gggagcggcg cctttagaag ggacaacgtc cggctcggga cttcagagcg ccgaggcgag
     1741 tcccgcgggt caggggtttt cgaagtccga atcgacgtct gccggttggc gcaatggctg
     1801 acgcagcgat tgctgagagg cggttttgcg gcgaatcacg gggcgcgtat gcggcggcgg
     1861 ggtaggaatt tgcggcaccg gcattgcccg ctggaccggc atcgggacat tgggcgccgg
     1921 ccgggcgggc atcggttgcg gtggcggtgg tggaatctgc gccaccggat tttgaatcga
     1981 ctggcttaag agcgcttcat tgaaagtaag ggtcgcgaac tgcccgtctt tcgaaatcgt
     2041 gaccttcgtc tcacccaccc gagtggacca ctggatattg gagatggaat aaccgtcgac
     2101 cgtttcccgg gtgctcaggt attccttgag attcttgtcg gtcgtcgaag cgagggtgac
     2161 aaaatcgccg tcggtcgaat gggcggcgtt ggcgacgtaa agatctttcg cgaaccccgg
     2221 cgcgcttgcc agcggcgcac cggcggtcgc gaccgcaaag ggcgaacgct tcaacatcgg
     2281 ctcgtaacgg gcgaacttct ccgtcgtcgg cagatcactg tcggcccgcg cgctggtcgt
     2341 taggcagagg agcggccagg tcaaaagcag cgcaagattt ttcgtcttca tcatttctgg
     2401 ccggccggtg cgaaccactt ggcgatcttg aagcggcccc gcatgacagt cgggtcaccc
     2461 gagtcgacca tcaagttgac cgtttcaaaa actataaaag cctccggtcg ctccgcatca
     2521 taaaaaaagt gcacgagggg cacccatccg cttttggttt cgaaggtggc cgagacggat
     2581 tgatgcgagg gcgtcgtctc gaccgcgccg atctgcggat catcaatctg gatgttgtat
     2641 ttgccggcga tctgtttcac ctgggtgagg agcgaggaag cttccgcggg gttagtcaaa
     2701 gtgggctggc ttttcttcaa ccactcggac cgtcttttcc aggttttctc ggcctcgagg
     2761 tagacttttt gttcggcccg cgccgcccgc tgggcggcga agtcggagcg cgcgcccgca
     2821 ctcatcccga ggagcgcgct ccagatcgcc agattgatga ggaggaaaag aatcgctccc
     2881 acggtgagcg cgagcatccg ctcccgttgg ttcatttggg ccagccagcg ggcgatcatt
     2941 tggtctttcc ttcgaggtga aattgggcgt ggtcgttagg caaaatccgc ggagtctcga
     3001 ggctgaaagt aaagtcctgc aggtcgggat gtttcttcac tttttcggca aactgatagg
     3061 cgagtgcggc cgagggcgct tccccctgga cggaaacgtt gcgcgccgac tgatcgtaaa
     3121 ccgtgatccg gacctcggga ttgggcaggc tctcgaagag gtgcaacaac acctcgatcg
     3181 ggtaataatg cggatcaatc gccggcgaaa gggctttcca ggcggcctgc gtattcttga
     3241 cgaactcgac tcgcggcgcg tcgcgccgga ccaccttgtc gaggtggcct acctgcagcc
     3301 gaatgatcac gacgtagagg gcgaagagca aaaacgccgc cgcgtaggcc ccgccggccc
     3361 agagcagacg ccggcgccac tccctttgcc tcgcagccgc ggcgcgctgc cggcgccaac
     3421 tctcgggcgc gaggttcagc ttcaccgtgg ccgggggagc ttccgtccgc atcagatcgg
     3481 ggcggagggc gaaaacgcgc tcgatcgtat cgcgcaacgg gaggcagttt tcatcgagca
     3541 ggacggtcgg gaaagccgtg tcgatcccct gcaactccgc gctcagggcc agctgcggca
     3601 gatcggtctc caaccgtttc ggctcggccg cgtcgaccgt gcggatgaga ctaagtttgc
     3661 cgttctcgct gatcgcggaa acgagtttct cgccttcccg ataaataatc agcgcgcgcc
     3721 cccgggcggc gtgcgatgcg gcgcgctggg cggcatagac cgtcacctgg cgcggaatgt
     3781 acccgcgact caggagtggc ccggccaatt ccgcgagcct cggattgtgg accgcgacgg
     3841 acgagataac gctgtcgctc tcgttttgct cgataatctc gaaatcgctc gtcacttcct
     3901 ccggcggata agggaacgct ttctccacct ggaggcgcac catggcgtca aactccgccg
     3961 ggtcgaccgt cggcaaacga agacgctggg cgaggactgc gttcaccggg agcgcaaaca
     4021 caatgtcgtc gccgccgttg agcagcccgg cggcttcgtt caagtcggcg acatgatgca
     4081 catcccaggt gccattgcct tcgcgtgccc ggaccagatt ccagccgctc ccggagggaa
     4141 ctataaaaac ggattcagat gcgggttcga tattacaatt ccttccaacg aatcacctgg
     4201 ggacttccgc tttttcgaat aatcatctgg atcgtgcgcg ttgcgttgcc ggattttccg
     4261 acgcttatca cgcgcaccac agggtctttg aatccgatca ggccggaaag ctgcgcaaat
     4321 tgctccggtg tgaatccgag tgcaagccta acctgttcga gcgattcaaa gaccggatca
     4381 tcctccgtgc cgtcgatccc atccggcccg cggcgatatt cgagaaatcg atcgacgatc
     4441 tgctcgttga atcctgggag ggagaggagg acttcgcgcg acgcccaggc gagatcgatc
     4501 gggccgctac tgtagagcgt aaagtccgcc tcccagtcgg gccgcgcggt aaattccgcc
     4561 cagcctttca cttgtttcag ctcctcgacg ctttggagga gtgcgttcct cgggtggtaa
     4621 tcctggtccg cctccgcgcc gttcagcctg gggatatcgt ccgggtcaac ccagtcgagc
     4681 aggcaatcca tcatccgttc gcgttcattg atatcgatgc ccttgttttc cagatatttt
     4741 tgcagaatcg cgagccgctg cggattttcg cccgacagga tccagttcaa attcaggcgc
     4801 ccaccttcac ccgtgatccg cgcctgatag gtctgattgg gggcaggcga gccgtgcaag
     4861 acggtcgagc cagcatcgac cgacgggtca agcgccacct cggcgccgga agcggccatc
     4921 gcttcggctt ccagaattcg gctcgaattc ccggcaagaa tgatgcggga atcgatgtcg
     4981 agcgcccacg cgatgaggac cgcgctgagc aggaaaagag cccagagcgc gaggagcaag
     5041 gcggcgccgg aacggctggc aaacgggccc tgtttcattt cctagagcgg cgtccgccgc
     5101 aacggaagga cggcttccca gggcaccgtg ctgcccgcgc ggtcaatcgt taggcgaacc
     5161 aaacacggca gcgcggtgtt atccgtccaa cgatcgaccc aggcattcaa acgcgaatcg
     5221 aaataacgga tctcgatgct gttgagattg gcgatcaacg gaatccacga ttggttttcg
     5281 cccgacgagt cgccgtcatc ctgtgactca cgcacgatcc ccaactgcat gcggttgccc
     5341 tttggcatcg gccggagccg gagcgtgacg gaatattcgc cggacgcata acgagtcagc
     5401 aaccccgggc ctgccgaaca aatccatgtc atttcgtcac gcgagcgcgc ctcgaatttg
     5461 aagggctcgc ccgagagggt cccctggccc ggcgggagat cctgcaactc ttcggccaac
     5521 agattggcca gtccggcata ctgggcgttg tcctcattca acgccgccga ggcctgcatc
     5581 gcgaccagat tcgcgctcac gaaacgataa atcgccagcg ccatcatccc aagaatcacg
     5641 accgcgagcg tgacctcgag gagggtgaag gcgcgacggc tgttctggtc aacccgcacg
     5701 gtagacataa aagaccagcc gcttgctttg ctttacaccc ccgcgcgacc actccgcgct
     5761 cagggtgacc cggctgatgc cgtcgatgat cgtattgtcc gcatcttcca attgttcggg
     5821 cacggttttc tggatcagcc taacgacacc atagccggta tccactttcg tctctttctc
     5881 ggcgtccggg tggacggggc tcgcctgcac ctccgccatc cgattcgcga gcagctggcg
     5941 cacccgattc tcttcctcgc tcagcgaact cgcccgcagg caattttcca ccgagcggcc
     6001 gagcgcgagc acgccgacca cgaagatcgt caggccgagc agcacctcgt agagcgcgaa
     6061 gccgggcaaa cccttatttc gtcgcataac gaacaatttc cgggcgcgcc gtcagaggcg
     6121 agtaattgac ttcccacgag ccgttcggcc cctggaagct gacgttcgcc ggctcgcacg
     6181 tgcccgatgg ccagaagatc cactgggcga gcggatcttt ttccagcgcg gccggcaacc
     6241 ggagctgaaa acggtggccc ttgtcgagca ccagtgtcgc ggcaggcgcg gccgagtctc
     6301 ctttggcaat cgattccggc cgcaggataa ccgtcttctt ttgccactcg atcaggtacg
     6361 gccggtgttc ctcgacgctg cgcttttgcg cttcgcgcac gatttgattc atcgcatcga
     6421 gcgacgtttt caggcgacgg tccgccagca ctccactcaa acttgggagg gcgaggagca
     6481 gcaggagcag caaaatgaaa accgaaatcg taatctcgat cagcgtaaaa cccatgcggc
     6541 gcggcatgag cgaattttac gccgacgatc ggaaatcaca accgacccct gtggccaggg
     6601 cgccggtgtg aaagcggccg gggttccctg gcggggctga aagcgcaatt taccgctaga
     6661 gaatagcaag cgcccggcag cgtttccgcg ttcgtgtgat aacatcgcta cgtgatttcg
     6721 cccgctgccc agccggtcgc tcctctcatc accgctgccg ctcccgcgct gcggctccga
     6781 ccgatgcaca aaatcctcct cgttcttgcc gcgctcggcg tgctcggggc gatgacctgg
     6841 cagggtctgg ctactggcgg ttcgcccgat cccacccgcg ccaacaccag ccccgccgtc
     6901 gccgtgctcg atattggtgt gctcgtcttc cgcgaagggc tcgaatgcat cctcgtcctg
     6961 gccgccatca ccgccagcat gaccggcccc cagcgcgatt gccgtcgacc ggtcgcctgg
     7021 ggcgccgccc ttgcttttct cgctactctc gtcacctgga tgatcgccgt ccgcatcatg
     7081 agcgcgctgg ccgataacgt ctcggccctc aacctgcaag cagccaccgg tcttcttgcc
     7141 atcgtcgtcc tgctcgtcat catgaactgg tttttccaca agatttattg gggcggctgg
     7201 attcgcgcgc acaatcggcg gcgcaaatct cttctcacca gcggcgccgc gactaatagt
     7261 tcgcagactc gtctcatctg gggcctgctc ctccttgggt tcacctcgct ttatcgcgaa
     7321 ggcttcgagg tcgtcctttt ccttcagact tattacctgc gtctcggcgg cggcgtggtg
     7381 ctgaagggcg cactcctcgg cctcgccctc tccggggtcg tcgccattct taccttcgtt
     7441 ctgcagcagc gactccccta ccgcaacatg ctcgtcacca ccggcgttct cctgggcggt
     7501 gtcctcctcg tgatggtcgg cgaacaagcg caggaaatgc aactcgcgca ttggatctcc
     7561 accacaccag tgccttcact cgttagtcgc gtgcccgatt gggccggtct ctggttcgcc
     7621 ctcttcccca cttacgaaac actcatcgcg caggcgatcg ccgcgctcgt cgtgctgggg
     7681 tcctactacg cggcccgtcg cgtccgctgc accgccgagc catcatgatc ctgaattgaa
     7741 aaacgggcgc tccgccttct tccccgggga gggacgattc actaaatatc tgtttggcgc
     7801 ccgcccgatt tcccgggagc tgcccgttgc agcccccggc ggcaacctgc gtccttccga
     7861 tattagcgaa gaccgccgct cgcgatcagc ct
//
LOCUS       DATMNS010000217         2907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2456035, whole genome shotgun sequence.
ACCESSION   DATMNS010000217 DATMNS010000000
VERSION     DATMNS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2907
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2456035"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VNW28_07595"
     CDS             complement(<1..403)
                     /locus_tag="VNW28_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09831.1"
                     /translation="MNAEQHRLEQNDHREERWHLWGPYLSERQWSTVREDYSANGDAW
                     NYFPHDHARSRAYRWGEDGIGGICDFKQRLCFAFAFWNERDPILKERLFGLTGPQGNH
                     GEDVKEIFFFEDNTPSHSYMRMTYRYPQAAFP"
     gene            571..1254
                     /locus_tag="VNW28_07600"
     CDS             571..1254
                     /locus_tag="VNW28_07600"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HXA09832.1"
                     /translation="MFTKKIRHWLSHLEAHHRLLISLSFALVVFLLVKSAFQWRIQLI
                     VTWDAFALCFLALAWARILTAQPRVAVRLTRLQPTSRKLIFVFVVAAACASLSAVAVL
                     LGMAKGMTGSRFATHIALALATVVMSWVVLHTVYSLHYAQLFYGEKKTGKSAGPAGGL
                     IFPGAQTEPDFLDFAYFSFVIGMTSQVSDVQISSPQIRRWALLHGIVSFAFNTAVLAL
                     SINVLSGLL"
     gene            1637..2623
                     /locus_tag="VNW28_07605"
     CDS             1637..2623
                     /locus_tag="VNW28_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXA09833.1"
                     /translation="MKSTQEITQQVQELGQWISPLREQVGHVVIGQKYLVDRLLLGLL
                     ANGHILLEGVPGLAKTLTVKTMAACIQTGFQRLQFTPDLLPADLIGTLIYNPRSGEFT
                     TKLGPIFSNLILADEINRAPAKVQSALLEAMQERQVTIGDKSYPLADPFLVLATENPL
                     EQEGTYQLPEAQLDRFMLKLNVGYPAKAEERRILDLMATSSPDLHVDPVVAPQQIIAA
                     RDLVNHIYIDDRVKDYIVDVVWATREPASYRLELEGLVRYGASPRATIYLALAARAYA
                     FLNGRGYVTPQDIKSIGPDVLRHRIIVSYEAEAESVTSETVIERIFAGLPVP"
     gene            2679..>2907
                     /locus_tag="VNW28_07610"
     CDS             2679..>2907
                     /locus_tag="VNW28_07610"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXA09834.1"
                     /translation="MSHQNYFVYILTNDDRHTVLYIGVTNDLERRANEHSLGRGSACA
                     KQYNAHKLIYFEAYPDPQSAIAREKQLKNWSR"
BASE COUNT          583 a          884 c          799 g          641 t
ORIGIN      
        1 agggaaatgc cgcctgcggg taacgatagg tcatccgcat gtaggagtgg ctcggcgtat
       61 tgtcctcgaa aaagaaaatc tccttcacgt cctcgccatg gttcccctgc ggaccggtga
      121 ggccgaaaag gcgctccttc agaatcgggt cgcgctcatt ccagaaagcg aaagcgaaac
      181 agagccgctg cttgaaatcg cagataccgc cgatcccatc ctcgccccag cgataagcgc
      241 gggaccgggc atggtcgtgc gggaaataat tccaggcgtc gccgttcgcg ctataatcct
      301 cgcgtaccgt gctccactgc cgctcactca ggtacggacc ccacagatgc cagcgctctt
      361 cacgatggtc gttctgttcg agacgatgtt gttcggcgtt catcaaagga cggtgattcg
      421 ttaggcgggt tcgccttctg attcaataat gaagtcggcg cctctgtcga tcaaaaccgc
      481 gtcccgccgt tgctccgcgg tccggttgca tttgttgtta agctccgagc gggttggatg
      541 tatggttgcc tcgctggcac gtcgaaaaaa gtgttcacga aaaagatcag acattggctg
      601 agccatctgg aagcgcatca tcggctgctc atttcgctca gcttcgccct cgtcgtcttt
      661 ctccttgtga agagcgcgtt tcaatggcgc atacagctca tcgtgacgtg ggacgctttt
      721 gcgctttgct ttctcgccct ggcctgggcg cgcattctca cggcccaacc gcgggtcgcg
      781 gtccggctga ccaggttgca gccgacgagc cgcaaactca tcttcgtttt cgtcgtggca
      841 gccgcctgcg cgagcctctc cgccgtcgcc gttttgctcg gcatggcgaa aggaatgacc
      901 ggcagccgct tcgcgactca catcgcgctg gcgctggcca ccgtggtgat gtcatgggtc
      961 gtgctgcaca cagtctattc gctgcattac gcgcaactct tttacggtga aaagaaaacc
     1021 ggaaaatcgg ccggtcccgc cggcggactg atttttcccg gcgcgcaaac cgagccggac
     1081 ttccttgact tcgcctactt ctccttcgtc atcgggatga cgtcgcaggt ctccgacgtg
     1141 caaatcagct cgccgcagat tcgccgctgg gcgctcctgc atggcatcgt ttccttcgcc
     1201 ttcaacactg ccgttctcgc cttgagcatc aacgtcctct ccggattgct ttaagcggct
     1261 gcactcggga aataggatct gaagatcctc cagttttgga acgacgcgca ccgccggact
     1321 tctgtgggca tttcattaat ttccccttcg attttgcgaa tgcacttggg tcgccctgtg
     1381 cacggttgac aggaaaatcg cgaatcgctc ccgcgactat gttccttccg cgaatggtgg
     1441 aaagaacatc atcgttgagg agcttcttca gcccgcgatc taatcgcagc taaattcaat
     1501 ttctagaagg gcgtcatgct tcagcttccc gccggctttc ttcaccacgc ggtgtcagtt
     1561 tggctttgtc ggccaccgga acctcgtttg ggcttttctt cgaacgcatt tcgtgcggat
     1621 ttgtctcatc atgcctatga aatcaacgca ggagattacc caacaggtcc aggagttggg
     1681 gcaatggatt tcgccactgc gcgaacaggt cggtcacgtc gtcattgggc aaaaatacct
     1741 cgtggatcgc cttttactcg gcctgctcgc caatggccac atcctcctcg aaggagtgcc
     1801 gggtctcgcc aaaacgctca ccgtcaaaac gatggcggct tgcattcaga ccggttttca
     1861 gcgcctgcaa tttacgcctg accttctccc ggcggacttg atcgggacct tgatttacaa
     1921 cccacgcagc ggcgagttta cgaccaagct cggcccgatt ttttcgaacc tgattctggc
     1981 cgatgaaatc aaccgcgcgc cggccaaggt gcagagcgcg ctgctcgaag cgatgcagga
     2041 acgccaggtc acgatcggcg acaagagcta tccgctcgcc gatccgtttc tcgtcctcgc
     2101 cacggaaaat ccgctcgaac aggagggcac ctaccaactg cccgaagcgc agctcgaccg
     2161 tttcatgctc aaactcaatg tcggttatcc ggcgaaagcg gaggagcgtc gcatcctcga
     2221 tctcatggcg acctcctcgc cggatctgca tgtagatccg gtggtcgcac cgcagcagat
     2281 catcgccgcg cgcgatctcg tgaaccacat ttacatcgac gaccgggtga aggattacat
     2341 cgtcgacgtc gtgtgggcga cgcgcgaacc ggcctcgtac cggcttgagc tggagggctt
     2401 ggttcgttac ggcgcctcgc cgcgcgccac gatctacctc gcgctcgccg cacgcgccta
     2461 cgcgtttctg aacggccgcg gctacgtcac gccgcaggac atcaagagca tcggccccga
     2521 cgtgctgcgt catcgcatta tcgtcagcta tgaagccgag gccgagagcg taacgagcga
     2581 gacggtcatc gaacggatct tcgccgggct gcccgtgcct tgagcgccgt catcccgagc
     2641 gcagtcgagg gatcccgtgg agtaagaaag aagctgtgat gagccatcaa aactatttcg
     2701 tttacatctt aacaaacgac gatcgccata ccgtgctcta catcggagtg accaacgatc
     2761 tcgagcgaag ggcgaatgaa cattcgttag gcagaggaag cgcttgcgcg aagcaataca
     2821 acgcacacaa gctcatttat ttcgaagcgt atcccgatcc gcagagcgca atcgctcgcg
     2881 agaagcagtt gaagaattgg agtcgga
//
LOCUS       DATMNS010000218         4468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2820135, whole genome shotgun sequence.
ACCESSION   DATMNS010000218 DATMNS010000000
VERSION     DATMNS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2820135"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VNW28_07615"
     CDS             <1..705
                     /locus_tag="VNW28_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HXA09835.1"
                     /translation="TPRFPIFGKLLDAEEKLSLQVHPGAAVAGALGGEPKTEMWYFAA
                     AEAGAEIYAGFRRGVERSRFEQALHNGDAAELVHRIAVKTGDAFFVPTGRLHAIGAGN
                     LLIEIQQNSDTTFRLFDWNRDARAGAQRPLQIEAALQSIDFDDFEPELIGPAGEQLVQ
                     CPHFAIEKWNLDGVRRASGRNTFALFVCLSGAVEASATRIAPGEFFLVPASAAATELQ
                     PAAPATSVLCITLPAR"
     gene            complement(737..1195)
                     /locus_tag="VNW28_07620"
     CDS             complement(737..1195)
                     /locus_tag="VNW28_07620"
                     /inference="COORDINATES: protein motif:HMM:NF027482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4864 domain-containing protein"
                     /protein_id="HXA09836.1"
                     /translation="MNRLAKISLLGFFFALCGAATLATHFARERTPPPATKELYSLVN
                     RQLSAFRAADFDGAYQHAATGVQQKFSRSQFELMIRRDFSPMTEPTQVEFGAVRVAGA
                     AALVQVFLTAPDGVVRGFLYSFTTEPDGWKIDGVQPLGAQPLRRLPGLRL"
     gene            complement(1198..1509)
                     /locus_tag="VNW28_07625"
     CDS             complement(1198..1509)
                     /locus_tag="VNW28_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HXA09837.1"
                     /translation="MRFEPATIEQIGSELAIQWNDGSETFLPLRFLRTACPCAACGGE
                     PDVMGELVRPSVSYTDQSFELRGWSLVGGYALQPTWGDGHNTGLYTYQYLRRIDPART
                     A"
     gene            complement(1548..2246)
                     /locus_tag="VNW28_07630"
     CDS             complement(1548..2246)
                     /locus_tag="VNW28_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXA09838.1"
                     /translation="MEEIAANLTRVEERIAEAAQKCGRSPDDIELVAVSKTHAAEKVQ
                     AAAEAGQMVFGESRVQEARAKIPLLPSRLRWHFIGHLQKNKIRHALPLFELFHGIDSL
                     ALAQDMQRIAEEDGMRPRILLEINLAGEASKHGFSPEALRRDLEATLSLGRLTIEGLM
                     TIPPLAPEAEAARPYFVALRKLRDQLEAEFNLRLPQLSMGMSSDYAIAIEEGATLVRV
                     GTAIFGERSRPKRD"
     gene            2360..3937
                     /locus_tag="VNW28_07635"
     CDS             2360..3937
                     /locus_tag="VNW28_07635"
                     /inference="COORDINATES: protein motif:HMM:NF045061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09839.1"
                     /translation="MRCAVGVCSLFLLAVATVILATEDGAVARKATPIREVQVPTWSQ
                     DDLNFFLHGSMGTEIFPEPVLRAFFKTYPELFPTTDLSHLGLIPDHDFGWPIGFSRKT
                     AVTHLGNLPAVGINCASCHVAQLTATTSDRPIRILGTTSAFDVEALFGSVLAATFKTS
                     DPANMKKFLALYLGAKPSVFEAAWRRQEGKIVAAMKDDPFGAKDIAPGRLHTIASADL
                     QPPFDKDTDLASRAHAMLQLFHNIRAVLHVPDQPPEKAPPASGPGRNDAFGILSAALL
                     NASQPYAPSKFGLVWNVDQRKWVHWDGNTRSPIARNLLASLGLGAPLYGHRADLNFAD
                     VQRQTDLTEEIRPPRYPFEIDPARAKRGAGHFAARCASCHTGPENDQRLHSVAEVGTD
                     PLRARLFNQTQADGFNRFLAGLELNGYVPPTEPGVRSTGKYFAASLGAVWARSPYLHN
                     GSVRTLADLLTRPNDRPKTFHRGSTRFDPTGLGYTDEGAYLFDTSTSGNSNAGHDYGT
                     DLSPNERQELIEYLKTL"
     gene            complement(4028..>4468)
                     /locus_tag="VNW28_07640"
     CDS             complement(4028..>4468)
                     /locus_tag="VNW28_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09840.1"
                     /translation="VHLIGTPHPSPAKAKQFREIDLVSVQVATKAAREAGVRHFVYLS
                     VAHPARAMQAFIAVRVEGETMLRASGMPATFVRPWYVLGPGHWWPYAVLPVYWLLERI
                     PQTRESAQRLGLITIGQMVTALVSAVENPPEQIRILDVPQIRQL"
BASE COUNT          802 a         1431 c         1415 g          820 t
ORIGIN      
        1 acgccgcgtt tcccgatctt cggcaagctg ctggacgccg aggagaagct ctcattgcaa
       61 gtgcatccag gcgccgcggt cgccggagct ctcggtggcg agccgaaaac cgagatgtgg
      121 tatttcgcgg ccgccgaggc gggcgcggag atttacgccg gctttcggcg cggcgtggag
      181 cgaagccgct tcgagcaggc tctccacaat ggcgacgcgg cggagctcgt gcaccggatc
      241 gcagtcaaaa cgggcgacgc ctttttcgtg ccgaccggtc ggttgcacgc gatcggcgcg
      301 ggcaacctgc tgatcgagat tcagcaaaac agcgacacca cttttcgcct cttcgattgg
      361 aaccgtgacg cgcgcgccgg ggcgcagcgc ccgttgcaga tcgaagcagc gctgcaatca
      421 atcgatttcg acgacttcga gccggagctg attggtccgg ccggcgagca gctcgtgcag
      481 tgtccgcatt tcgccatcga gaaatggaat ctggacggcg tccgccgcgc atccgggcgg
      541 aacacgtttg cgctcttcgt ctgtctcagc ggcgccgtgg aggcgagtgc gacccgcatt
      601 gcgccgggcg agtttttcct tgtcccggcg agtgcggccg caaccgaatt gcagccggcg
      661 gcgccggcaa ccagcgtgct gtgcatcacc ttgcccgctc gttaggggag ttcttctgga
      721 gcccgcctca ggcgggctac aggcggagcc ccggcagacg ccggagcggc tgtgcgccca
      781 acggctggac gccatcgatt ttccagccat ctggctcggt cgtaaaactg tagaggaagc
      841 cgcgcacgac gccatcaggc gcggtgagga aaacctggac cagggctgcc gcgcccgcca
      901 cgcgaacggc gccgaattcc acctgcgtcg gctcagtcat cggcgaaaaa tcgcgccgaa
      961 tcatgagctc gaactgggaa cgcgaaaatt tctgctggac cccggtcgcg gcatgctgat
     1021 aggcgccgtc gaaatcggcg gcgcgaaagg cggagagctg ccggttaacg agcgaataaa
     1081 gttccttcgt agccggcggc ggggtccgct cgcgcgcaaa gtgggtcgcg agggtggccg
     1141 cgccgcacag ggcgaagaaa aatccgagaa gagaaatctt ggccagccgg ttcatcgtta
     1201 ggcggtacgc gccgggtcga tccggcgcag atactgataa gtgtagagtc cggtattgtg
     1261 gccgtcgccc caggtcggct ggagcgcata accgccgacg agactccagc cgcggagttc
     1321 aaaactttgg tcggtatagc tgacgctagg gcgaacgagt tcacccatca cgtccggctc
     1381 gcctccgcag gccgcgcagg ggcaggcggt gcgcaggaag cggagaggca gaaacgtttc
     1441 gctcccgtca ttccattgaa tggcgagttc gctcccgatc tgttcgatgg tggccggctc
     1501 gaagcgcacg ctaaagccta acagggttct gcggtggagg gcaactctca gtcgcgtttc
     1561 ggtcgcgaac gctcgccgaa aatcgcggtg ccgacccgca ccagggtcgc cccttcctcg
     1621 atcgcgattg cgtaatcgct gctcattccc atcgaaagtt gcggcagcct gaggttgaac
     1681 tccgcttcga gttggtcgcg cagtttgcgc agcgcgacaa agtaagggcg cgctgcctcc
     1741 gcttccggcg cgagcggcgg gatcgtcatc agaccttcga tggtcagtct ccctaacgac
     1801 aaggtcgctt ccaggtcgcg ccgcagtgcc tccggcgaaa agccatgctt gctcgcttcc
     1861 ccggccaggt tgatctcgag caaaatgcgc ggccgcattc cgtcttcctc ggcgatccgt
     1921 tgcatgtcct gcgccagcgc gagcgaatcg atgccgtgaa acaactcgaa gagcggaagc
     1981 gcgtggcgga ttttgttctt ttgcaggtgc ccgatgaaat gccagcgcag gcgcgaaggc
     2041 agcagcggga tcttggcccg cgcttcctgg acccggcttt cgccaaagac catttgcccc
     2101 gcctcggccg ccgcctgcac cttctccgcc gcgtgcgtct tcgagaccgc gaccagctcg
     2161 atgtcgtcag gcgaacgacc gcacttttgc gccgcttcgg cgatgcgctc ctcgacgcgc
     2221 gtaaggttgg ccgcaatttc ttccatcggc gaattattca ttgcccgagg acggcggcaa
     2281 gcaatctccc gcgacggcgg gcttaccgaa ccaatcgatt gggattgaaa actggctttg
     2341 cgcccgataa atcgttgcca tgcgttgcgc cgttggggtg tgcagcttgt ttctgcttgc
     2401 cgtggcaacg gttatcctcg cgaccgaaga cggcgcagtg gcccggaagg ctacgccgat
     2461 tcgggaagtg caggttccga cctggtcgca ggatgatctg aattttttcc tgcacggctc
     2521 gatgggcacg gaaattttcc cggaaccggt cctgcgggcg tttttcaaaa cttatccgga
     2581 gctctttccg acgacggacc tgagtcatct tgggctgatt ccggatcacg actttggctg
     2641 gcctatcggg ttcagtcgca agaccgcggt cacacatctg ggcaatctcc cggccgtcgg
     2701 gatcaactgc gcgtcgtgtc acgtcgcgca gcttaccgcg acgacaagcg accgaccgat
     2761 tcgcattctc ggcacgacga gcgccttcga tgtcgaagcg ttgtttggct cggtgctggc
     2821 cgcgactttc aagacgtccg acccggccaa catgaaaaaa ttcctcgccc tttatctcgg
     2881 cgcgaagccg tcggtcttcg aggcggcgtg gcgccggcag gagggcaaga tcgtcgccgc
     2941 gatgaaggac gatccgttcg gcgcgaaaga catcgcgccg ggccggctgc atacgattgc
     3001 gagcgccgat ctgcagccgc ccttcgacaa agacaccgat ctcgcctcgc gtgcgcatgc
     3061 aatgctgcaa ctgtttcaca acatccgcgc ggtcctgcat gtgccggacc agccgcccga
     3121 aaaggcgccg cccgcttccg gccccggccg gaacgacgcc ttcggcattt tgtcggccgc
     3181 gctcctgaac gcctcgcagc cctacgcgcc gagcaaattc ggactggtct ggaatgtcga
     3241 tcagcggaag tgggtgcatt gggacggcaa cacgcgttca ccgatcgcgc gcaacctgct
     3301 cgcctcgctc gggctgggcg cgccgctcta cggtcatcgc gccgatctga attttgcgga
     3361 tgtgcagcgg cagaccgatc tcacggaaga aatccggccg ccgcgttatc cgtttgagat
     3421 cgacccggcg cgggcgaaac gcggcgccgg ccacttcgcg gcgcgctgcg cgagttgtca
     3481 taccgggccg gaaaacgatc agcgtctgca ttcggtcgcg gaagtcggga cggacccgtt
     3541 gcgcgccagg ctttttaacc agacgcaggc cgacggcttt aatcgcttcc tcgcggggtt
     3601 ggagctgaac ggctacgtgc caccaacgga acccggagtg cgatcgaccg gaaaatattt
     3661 cgcggcctcg ctcggggccg tctgggcgcg ctcgccctat ctgcacaatg gttcggtgcg
     3721 aacgctggcg gatctgctga ccaggcctaa cgaccgaccg aagacattcc atcgcggctc
     3781 gacccgcttc gatccgactg gactgggtta caccgacgaa ggcgcctacc ttttcgatac
     3841 cagcacctcc gggaattcga atgccggcca tgattacggg actgaccttt cgcctaacga
     3901 gaggcaggag ttgatcgaat atctgaaaac gctctgaccc gtggcctccg gagggacgtg
     3961 cttccgcacg tccggaagag agcctgcgcg tcgagttgat ccatgcgccg cccacaatgg
     4021 gcggcgtcta caattgacgg atctgcggca catcgaggat ccggatttgc tccggcggat
     4081 tctccaccgc cgagacaagc gcggtcacca tctgcccgat ggtgatcagc cccaggcgtt
     4141 gtgctgactc cctggtttgc gggattcgtt ccaggagcca gtaaaccggc agcaccgcgt
     4201 agggccacca gtgcccgggc ccgaggacat accatggcct aacgaacgtc gccggcatcc
     4261 cgctggcgcg cagcatcgtc tcgccttcca cccgcacggc gatgaacgcc tgcatcgcgc
     4321 gcgccggatg ggccacgctc aggtagacaa aatgccgcac cccggcctcg cgggccgctt
     4381 tcgtcgcgac ctgcaccgag acaaggtcga tttcccggaa ctgtttcgcc ttcgccgggc
     4441 tcggatgcgg cgtgccgatc aaatggac
//
LOCUS       DATMNS010000219        21357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5322246, whole genome shotgun sequence.
ACCESSION   DATMNS010000219 DATMNS010000000
VERSION     DATMNS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 21357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21357
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5322246"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VNW28_07645"
     CDS             <1..403
                     /locus_tag="VNW28_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09841.1"
                     /translation="QQLERTRAYVPTKVLLGDALGYFDPDSLYEMLHCIIGLTNNRFI
                     FLATSIPCDELKWSFYDTAERVRRYEKNQLLPGNTNDGVGRWWKKEELEQVGRRLGLK
                     VGLRELPPMLSTFRIDAAFVSGPEVVQRAAR"
     gene            407..1345
                     /locus_tag="VNW28_07650"
     CDS             407..1345
                     /locus_tag="VNW28_07650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09842.1"
                     /translation="MNYELEVRVPISPTPDFFRRVHFMAASLQRLEAKLGSYLLVVCV
                     GGDVQPYDLYEAERWSKNYPVIWRWADRRKFQRDSFWETSREIFRQPIHGRVVMCADA
                     DVIFVRDFSDLLRELEAAPAVAGVIAHAPPIRGPALAGLWPRLCEGYKVPLPPPVHEY
                     TGWNFMTRDQMTPVYYNFGMVLVPARLMEVLSPEMEPADDFVNANLETFFRFQIALTL
                     AIQKAGLPSRTLRLRDNFPNDPQFDERYPVELEEIRILHYLRCEIVHREKDFAHLDGV
                     AALIARTDLKGSNAVLRETLERLYPIVAAGEKAAVT"
     gene            complement(1338..2321)
                     /locus_tag="VNW28_07655"
     CDS             complement(1338..2321)
                     /locus_tag="VNW28_07655"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXA09843.1"
                     /translation="MNSPIKVAVTGAAGQIGYSLLFRIASGAMFGPDQPVHLSLIEIP
                     PALGALEGVVMELHDCAFPLLKGITPTADLDEGFRGVNWALLVGSIPRKAGMERGDLL
                     GINGKIFIGQGQAIAKNAASDVRVLVVGNPCNTNCLIAMNNAKSIPRDRWFAMTRLDE
                     NRAKAQLAQKAGVDITAVTNMTIWGNHSATQYPDFYNARINGKTADQMIGDEKWLKET
                     FIPAVQQRGAAVIKARGLSSAASAANAVVDTVRSLTTDTESGNWHSVAICSDGSYGVE
                     KDLISSFPVHTTGGRWEIVQNVVLNEFSRAKVDASVAELKEEKALVGELIK"
     gene            complement(2391..4136)
                     /locus_tag="VNW28_07660"
     CDS             complement(2391..4136)
                     /locus_tag="VNW28_07660"
                     /inference="COORDINATES: protein motif:HMM:NF014499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HXA09844.1"
                     /translation="MTISPNEKIAGVLAPLFALRTENDLGIGDVAGLRQFIAWARTIG
                     FKLVQLLPINETGGDHSPYNAISSRAIDPTTIELAPGVPEDLSAQAFAEVTAGIDLAA
                     LRRGRVKYGIVKPLKKKLLERAFEAFATGARTDAATKEAFASFCEAEKSWLADYTLFR
                     VLMERNGDRETWDEWRAEHSEIDAAHRWLDSLGEEERAPFAERREFYNYVQWIAHRQW
                     REVKKYAEGQGVALMGDIPFGISYYSADVFAHPDRFALDWSGGAPPEPYFKDDAFTQK
                     WGQNWGIPLYRWEVMRTRHFDWWRQRVRGVREIFHLFRIDHVLGFYRLYAFPWRPCHN
                     PAFLPLAWEEMRARTDGREPHFMPRDDRSWENCEANKREGEEYLRIVLEESGATRVVG
                     EDLGTVPDYVRPSLCSLGIAGFKIPQWENYPDQRSIPGSAYERLSVTTFATHDHKPLR
                     ALWEEAFETPSGSAEQARHDLAKIAEFAGLASPHAGMVYPQDFYPAIFEALFRSESWI
                     ALVMITDLLGRKERFNVPGTAANTNWTRRLSTTVGGLGRSRALKRQMRLVRELLEKSG
                     RIVIPSATGGSTEVG"
     gene            complement(4222..6288)
                     /locus_tag="VNW28_07665"
     CDS             complement(4222..6288)
                     /locus_tag="VNW28_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXA09845.1"
                     /translation="MSREYTLQRAPSAATINIDYGNELNEQQYAAVTAPPGPLLVIAG
                     AGSGKTRTLTYRVAYLLENGVDPRNILLLTFTNKAARQMLDRVANLLPVDASGLWGGT
                     FHSIGNRMLRRHGSALGYSSGFTIMDREDQKDLLNSVVAGSGIDPKEIRFPKGEVLAD
                     IFSFVVNTERPIEELLAEKFPYFLPLLEQIKEVHLRYERRKKSTNSLDFDDLLEKTLR
                     MLKEHEEIAAFYRRQFQFILVDEYQDTNKIQADLIDLLAAEHQNVMVVGDDAQSIYSW
                     RGANFQNILAFPRRYPGAQVFKIELNYRSVPEILQVANAAIAANLQQFRKQLSATRLT
                     NSLKPAIVALNDGGEQSQFIAQRILELRDEGIELHEIAVLYRAHYHAVELQLELARRG
                     IPYQITSGIRFFEQAHIKDVAAFLRFVANPRDEVAFKRMVRLLPGIGGKSAENLWRSW
                     EESLGEATPVVTAGADPGSVGAEISARIADPGYNFGERLRALPVGARSKKSWEQLAWT
                     LDEIAPRGRPNQPTEMIMSIVEAIYDDYAKANFANYELRKEDLTQLAAFARQFKDIHE
                     FLSQLALISNLDAEPATNQTGDTEAVNLSTVHQAKGLEFHSVFVIWLTDGMFPSTRSL
                     ETRDAIEEERRLFYVAITRAKDELYLTYPHLRLNAGYGDMFQRPSRFLKEIPLSLVED
                     WNVRRG"
     gene            6558..7004
                     /locus_tag="VNW28_07670"
     CDS             6558..7004
                     /locus_tag="VNW28_07670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA09846.1"
                     /translation="MKILNRDRAEFLVIDDDPAITRFLAAYLKQQGHTCATLTEGFQT
                     ASWLELHDCEVVVVDLNMPKVDGISLITYVREIQPSLPIIVFTGVGYDEEKMHAALRA
                     GANGYVSKNLPIEQLYCVLSRVLATCRQRARREALARARPALLGAA"
     gene            complement(7001..7405)
                     /locus_tag="VNW28_07675"
     CDS             complement(7001..7405)
                     /locus_tag="VNW28_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXA09847.1"
                     /translation="MRPQYLLVDGHSIIFAWPELRLLHERRTSLAREALIRQLRDYQD
                     WSGVRVVVVFDGRGTAVSHEAEPGEIQIFYSRHGQTADSIIERLTSKYAERFDLVVAT
                     GDYLEQETASASGAETVSPEGLRDLLQEARPR"
     gene            7801..8562
                     /locus_tag="VNW28_07680"
     CDS             7801..8562
                     /locus_tag="VNW28_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09848.1"
                     /translation="MALSLFFGFLNSSKLKGLRNESVSTTSARETADRARMASERKLK
                     AREVEFAKAASKATAAEAKATSAETDLSKTQNEKAELKSKLQASEGQIADLQKRVAEA
                     TVGEVPGASEGVSPNELKALLDDTKHQLVAAEQEKSILADKVRAAQDRVAAIEEEKKR
                     RESGVNAPGVHGTILAVNQAYNFVVLSMGDRQGVVPNSEMLVMRRGALIGKIRVSSVE
                     PTTSIGDIITNSLARGVQVQPGDTVIYAGSNNSSS"
     gene            complement(8810..10111)
                     /locus_tag="VNW28_07685"
     CDS             complement(8810..10111)
                     /locus_tag="VNW28_07685"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HXA09849.1"
                     /translation="MPQSPALPPGTTQRTLSIIMGGGAGTRLFPLTKDRSKPAVPLGG
                     KYRLVDIPISNCLNSGLRSIYVLTQFNSMSLHRHIQASYKFDNFSRSFVDILAAQQTP
                     EGAQWYQGTADAVRQNLRYFLEQPYDYFLILSGDQLYRMDFRTLLHQHIRSGAEITLA
                     TTPVQRAAASDFGIMLSGPDRRITRFEEKPKETAVLDELKMDRELLNSIESDPDEELF
                     QASMGIYVFNRQVLVDCLNSDLDDFGKHVIPRAIGERHVNAYIFKGYWEDIGTIRAFY
                     EANLDLTNLAPNYSFFEAQAPIYTHPRFLPGSKINGATLRQAIISDGCIISDAHIERS
                     VIGVRSIIRSGATIRNSVLMGGDHFEGAAPAKSPDLPPIGIGRNCVIDRAIIDKNARI
                     GDGVVITPEGKEANLDAPNYYIRDGIVVVPKDAVIPAGFWI"
     gene            10306..12177
                     /gene="glmS"
                     /locus_tag="VNW28_07690"
     CDS             10306..12177
                     /gene="glmS"
                     /locus_tag="VNW28_07690"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXA09850.1"
                     /translation="MCGIVGYVGRAEAAPILLDGLRRLEYRGYDSAGVAIVDRDHVET
                     RKCAGRIAGLSQLIAEHPPSGTYGISHTRWATHGKPTDQNAHPHFDESGKLALVHNGV
                     IENYQQIKDALIQESPHHFTSETDTEVLAHLVGKIYDDSAVGVVDPRRHKALLVEALR
                     TALQQVIGTYGIALVHADIPDFIVGARRGSPLVLGVGKDENFLASDVSAIVAHTRDAV
                     YLNDFDLVAVERDNFEISSLAGERGSHAISKVEFTAEDIKKGDYPHYMLKEIFEQPGS
                     VRDAMRGRLSVEECTAKLGGLNMTPAQLRDVGRVVLTGCGTALHAGMVGEYLIEQLAH
                     IPVEVEYASEFRHRNTPMTTDTLVFAISQSGETADTLGALRESKRKGHRTLGICNNVA
                     STIARESDGGVYMHAGPEIGVAATKSFTSQVVILTLIGLLLGRMRHLSTAEGTSISGA
                     LEALPEKIEQILKMSDQIREVAKKYAQATGMLFFGRQFNFPIAREAALKLKEITYLYA
                     EGHPSAELKHGVIALVKPELPSVFITPDDAVFSKNMNNIEQVRARKGPIIAVTTASGA
                     AKLKGIADDIITVPDADEIVMPVLTVIPLQLFAYHLAVALGRDVDKPRNLAKSVTVE"
     gene            complement(12316..12819)
                     /locus_tag="VNW28_07695"
     CDS             complement(12316..12819)
                     /locus_tag="VNW28_07695"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HXA09851.1"
                     /translation="MLTSKKVVNAINKQIGNEFGASLQYVAIAAYFSGDALPQLAEHF
                     YRQAAEENDHAMRFIKYVVDAGGRVVIPAIAAPQPTFKNAEEAIRLSLDQEIVVTRQI
                     NALVELARSENDFITNNFLQWFLTEQLEEVSSMDTLLKIVQRGANNLLSVEEYLARVG
                     VKGAGPS"
     gene            complement(12971..13225)
                     /locus_tag="VNW28_07700"
     CDS             complement(12971..13225)
                     /locus_tag="VNW28_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004946303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXA09852.1"
                     /translation="MGAILWTLFIGLVVGAIAKLLMPGKDPGGCIITILLGVAGAFVA
                     GYLGRVLGWYQPGQPAGFIASIIGAMLLLLIYRIIAGKRS"
     gene            complement(13273..14865)
                     /gene="serA"
                     /locus_tag="VNW28_07705"
     CDS             complement(13273..14865)
                     /gene="serA"
                     /locus_tag="VNW28_07705"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXA09853.1"
                     /translation="MSAPKVLIADSISQRGIDELAAGAALDVTVHTGLSEAELVQLIP
                     EFSALVVRSRTKVTAPILQAGKCLRVVGRAGVGVDNVDVEAATRRGVIVMNTPGGNTI
                     STAEHAFSLLVSVARKIPQAHSNLQAGKWDRKQFEGTELYNKTLGIIGMGRIGSELSR
                     RAIAFGMRVLAYDPYLSSSRARSLQVELVEELDELLPLADFLTLHTPLTPETHHLLNA
                     ARLTKTKRGVRLINCARGGLIDEVALLESLGNGQVAGAALDVFEQEPLPADSPLRGIP
                     NLILTPHLGASTVEAQESVGIEIAQAIRAALLEGTIRNAINLPNLDAKTLAIVGPHLR
                     FGEKLGRFLSQVSPKRVENLHINYSGKVNEVDTTAITRSILKGFLHLAGGEDVNEVNA
                     PAFAETLGMKVTESRLSAPGDYTDLLELTAEAEGKTLSVAGAFFGATPRIVSINSRPV
                     EARPHGVLLVLENTDRPGMVGRIGTLLGAHDVNIATMSLSRNQAGGRALTVLNLDTAP
                     PPELLVEIAASDDIQSAQVVEL"
     gene            complement(14885..16780)
                     /locus_tag="VNW28_07710"
     CDS             complement(14885..16780)
                     /locus_tag="VNW28_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXA09854.1"
                     /translation="MAEPFIATPGVHRPRNVDWKRAAALLYGDWGTSKAYVIGLAFLA
                     AGFSSLPIILAVCVVTALVGVNYIVICRHFPDGGGVYSAARSQGRLLAVVGALLLLAD
                     LTVTAALSGWAGLSYITSGAEQVVWIKMLRDHIGLTTIAVLIIMGWLNYFGPKHSGSL
                     AVVLSVPTAIVVLVLIGVSAPHLTTQFLEPRHQHFSALWVQFVGVILALSGAESIANL
                     TGVMKLDRGSTMDKPSVARTSLQAILPVAIEVVAGTALLGWAMLSLPSVLPQTLHLTD
                     KLQIRAAMGARNEDMLRFIGEQFGAATVSPWFGVMFGWVVGIVFFFLLVSAANTAIVA
                     MIGLLFMMARDGEMPRQFTRLNKYGVPIYPLFIAVGIPSLVLLTATGFTSLAGLYAIG
                     VVGAIAVNVGSCALNRTAGFTWYDRVLFGITFVILFLVELTLAHSKPDALFFVMCVLL
                     GGLALRAYTLKRQGLTTLTVTREVADMVSPQTLAAFAPRLEEGTKIMVAARGVTPVLN
                     FALEEAELRKAVLCVLYVREIAVYFSGGPATLGRVRWQDDPVAQAIMSSMLKQGQDRG
                     VCVLPVYAVSEDAAATILDLSATMGVDYLIIGTSQRSTLAQLLGGSLVTRVAAELPDT
                     IRLVIFG"
     gene            16925..17155
                     /locus_tag="VNW28_07715"
     CDS             16925..17155
                     /locus_tag="VNW28_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09855.1"
                     /translation="MRTTITLDRDVFEAARTLAASSGKKLGQVVSQLARRGLRAPPDV
                     AAKNGLPVFRVSPDAPVIPSDRARDLLADEET"
     gene            17152..17568
                     /locus_tag="VNW28_07720"
     CDS             17152..17568
                     /locus_tag="VNW28_07720"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HXA09856.1"
                     /translation="MKPALLDTNVLLALAWPNHQHHAETHQWFGAQSRHGWATCALTQ
                     LGFIRLSANPAYTPAAVSPQEAASLLERLLRHESHHFWPCPAATAPAIYSRTLGHQQT
                     NDAYLVEVVRRHKGRLVTLDRRASAHGDEVVFVIAG"
     gene            18281..18661
                     /locus_tag="VNW28_07725"
     CDS             18281..18661
                     /locus_tag="VNW28_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09857.1"
                     /translation="MIFTIGRAATILALCLSLGLHWVALQSVAWTTMLVENACHVPLS
                     DAVAKTFDGNHPCDLCHMVATGQKSEKKSEMLPTVGKMDLICTTRTLSWLPPWVSYDY
                     PRINSAIPERSQAPPVPPPRLLPG"
     gene            18780..19700
                     /locus_tag="VNW28_07730"
     CDS             18780..19700
                     /locus_tag="VNW28_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09858.1"
                     /translation="MKHFVLLFWLTCAACEFGATSRAYGHGFAGARFFPATLSTDDPF
                     VADEFSLPTVSSIITPDNGGTRDTEISSDIALRITPKWDIEFGETFITLNPSQGKVVN
                     GFDNLSIGSKCEFFENDEHETVASLGLDVDVGGTGSKQLGADTFSTWTPGLFFGKGLG
                     DLPQGLSLVRPVAVTGLVGVAVPGRASTDGSPNPAVLQLGFAFEYSIIYLQEQVRDIG
                     LRAPFDRLIPLVEVSLSDPLDRGESGLITGTVNPGVIWSGKYFQVGAEAVIPINSRTG
                     NNIGFIAQLHFYLDDLFPHSLGRPLFGGNK"
     gene            19697..20053
                     /locus_tag="VNW28_07735"
     CDS             19697..20053
                     /locus_tag="VNW28_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HXA09859.1"
                     /translation="MKRIILSFVLIVLAGSASLEAHAFLKGAEPSVGSTVQTAPNEVR
                     IRFTENIEPAFSKIQVFNLSGNEVDKRDVHLDRSDPAVLQVSLSPLGSGTYKVVWRVV
                     SVDTHVTEGSFSFHVR"
     gene            20078..21040
                     /locus_tag="VNW28_07740"
     CDS             20078..21040
                     /locus_tag="VNW28_07740"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HXA09860.1"
                     /translation="MLDALLIVARSAQIAASILFAGVFTFKVVALGRVGQSAGDAGQA
                     LDRQFFWLALWSLVAALFSALLWFWLVVASMSGSSLADALSGNAWQMVLSQTEFGRVW
                     QLRLAVIGLALALVAVGLPRKNSRHALTLALWPLALVLLVSLAWISHAAAARSQPLGL
                     LGDALHLCAAGGWIGGLWPLAIFLKRTGVSVSSGAPALHVLRRFSTLSLCSVGVLVVS
                     GLSNSWLLVGSVAALFTTRYGALLLFKLTLFALLLGFGARNRGIINTAAISPGLLAQL
                     RRNVLYEICLGLAVVAIVGWLGITPPARSGTSAENRTLAISSSR"
     gene            21124..>21357
                     /locus_tag="VNW28_07745"
     CDS             21124..>21357
                     /locus_tag="VNW28_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09861.1"
                     /translation="MSRREKLQAFVASGLLSAFLFALALAASPQLHQRLHADAGSPDH
                     ECAATLIAAGKYEQADAPPVVVAPQPALVFERIP"
BASE COUNT         4140 a         6381 c         6547 g         4289 t
ORIGIN      
        1 ccaacaactc gagaggacgc gagcctatgt tccgacaaag gttctcttgg gagacgctct
       61 cggttacttc gatccggact ccctttatga gatgcttcac tgcataattg gcctaacgaa
      121 taataggttc attttcctgg ccacgagtat cccgtgcgac gagttgaaat ggagtttcta
      181 tgacacggcg gaacgggtgc ggcgctatga gaagaatcaa ctcctgccgg gcaacacgaa
      241 cgacggggtg ggccgttggt ggaaaaagga agagctggag caagttggtc gccggctggg
      301 tttaaaagtc ggcctgagag agttgccgcc gatgctttcg acttttcgca tcgatgccgc
      361 ctttgtcagt gggcctgaag ttgtccagcg cgcggcgcgt tgaatgatga actacgagct
      421 ggaagtgcgt gttccgatca gtccgacgcc ggacttcttt cgtcgcgttc atttcatggc
      481 ggcatcactg caacgccttg aagcaaagct cggatcttat ttgctcgtgg tttgcgtcgg
      541 cggcgacgtg caaccatacg atctttacga agcggaacgc tggtcgaaga attatcctgt
      601 gatctggcgc tgggccgatc gccggaaatt ccagcgcgac agtttttggg aaaccagccg
      661 ggagattttc cggcagccga tccacggccg tgtcgtgatg tgcgcggatg ccgacgtgat
      721 cttcgtgcgc gatttttcgg acttgctgcg cgagttagag gcggctccgg ccgtggcggg
      781 cgtgatcgcg cacgctccgc cgatccgcgg tccggcgctg gccgggctct ggccgcgact
      841 ttgcgaggga tacaaagtgc ctcttccgcc gccagtccac gaatacaccg gctggaattt
      901 catgacccgc gatcaaatga ctccggtcta ttacaacttc gggatggtgc tggtgccggc
      961 gcgcttgatg gaggtgctta gcccggagat ggagccggcg gatgattttg tgaacgccaa
     1021 tctcgagacc tttttccgct tccaaatcgc gctcacgctc gcgatccaaa aagccggttt
     1081 gccgagccgt acgcttcgct tgcgcgacaa tttccctaac gacccgcaat tcgacgaaag
     1141 atatccggtc gagctggagg agattcgcat cctgcattat ctgcgttgcg agatagtcca
     1201 tcgggaaaag gattttgccc atctggacgg cgtcgcggca ctgatcgcca gaaccgacct
     1261 caaaggcagc aacgcggttc tgcgcgagac cctggaacgg ctctacccga tcgtcgcggc
     1321 cggcgaaaag gcggcggtta cttgatgagc tcgccgacca gcgctttctc ctccttcagc
     1381 tccgcgacgg aagcatcgac tttcgcgcgg ctgaactcgt tcagcacaac attctgcacg
     1441 atttcccagc ggccgcctgt cgtgtggacg gggaaggaac tgatgaggtc tttttcgacc
     1501 ccgtagctcc cgtcggaaca gatcgcgacg ctgtgccagt tcccgctttc ggtgtccgtc
     1561 gtcaggctgc ggaccgtgtc cacgactgcg ttggcggcgg aagcggcgga ggaaaggccg
     1621 cgcgccttga tgacggcggc gccgcgttgt tggaccgcgg ggataaaggt ttccttcaac
     1681 catttctcgt cgccaatcat ctgatcggcg gttttcccgt tgattcttgc gttgtagaaa
     1741 tcgggatact gcgtcgcgga atggttaccc cagatagtca tgtttgtgac cgcggtgatg
     1801 tcgacgcccg ctttctgcgc gagctgcgcc ttggcgcggt tttcgtcgag gcgagtcatg
     1861 gcaaaccagc gatcgcgcgg gatcgatttg gcgttgttca tcgcgatgag gcaattggtg
     1921 ttgcagggat tgccgacgac cagaacgcgc acatcagagg cggcgttttt cgcaatcgcc
     1981 tggccctggc cgatgaaaat tttgccgttg atgcctaaca aatcgccgcg ctccatcccg
     2041 gccttgcgtg ggatgctgcc gacgagcagc gcccaattta cgccccgaaa accttcgtcg
     2101 aggtcggcgg tcggcgtaat gcccttaagg aggggaaagg cgcagtcgtg cagttccatg
     2161 accacgcctt cgagcgcgcc gagcgccggc ggaatttcaa tcagcgacaa atgaaccggc
     2221 tggtcgggac cgaacattgc cccggaggcg atccgaaaga ggagcgaata tccgatctga
     2281 ccggcggcgc cggtaacggc gactttgata ggtgaattca tcatatttga taagcgcaga
     2341 taggctcggt gtcattccgg gcgaagccgc cggatcccga ggcggagatc tcagccaact
     2401 tcagtggatc ctccagtcgc gctcgggatg acgatgcgtc cggacttttc cagcaactcg
     2461 cgcacgagac gcatttgccg cttgagagcg cgactgcgac ccaatccgcc aaccgtggtg
     2521 gagaggcgtc gcgtccaatt ggtgttcgcc gcggttccgg gaacattgaa ccgctccttt
     2581 cgcccaagga gatccgtgat catgacgagc gcgatccacg attcgctgcg aaagagtgcc
     2641 tcgaaaatcg ccgggtagaa atcctgcggg taaaccatcc ctgcgtgagg ggaagcgagc
     2701 ccggcaaact ccgcgatctt cgcgagatcg tggcgcgctt gttcggccga gccggatggc
     2761 gtctcgaaag cctcctccca gagtgcgcgc aagggcttgt gatcgtgggt cgcaaaggtc
     2821 gtgaccgaga ggcgttcgta ggcgctcccc gggatcgatc gctggtcggg ataattttcc
     2881 cattgcggaa tcttgaagcc ggcgatgcct aacgaacaca aactcgggcg cacgtagtcg
     2941 ggcaccgttc cgagatcctc tcccacgacg cgggtcgcgc ctgattcctc gagcacgatg
     3001 cggaggtatt cttctccctc gcgtttgttc gcttcgcagt tttcccaact gcgatcatcg
     3061 cgcggcataa aatggggctc ccttccgtcc gtgcgcgcgc gcatttcctc ccaggccaga
     3121 ggcaggaacg ctgggttgtg gcacggacgc caggggaaag catagaggcg gtaaaatccg
     3181 agcacgtgat cgatgcggaa aaggtggaaa atttcccgca ccccgcgcac ccgctgccgc
     3241 caccagtcga aatggcgggt ccgcatcacc tcccagcgat aaagcgggat gccccagttc
     3301 tggccccact tttgggtgaa ggcatcgtcc ttgaaataag gctcgggcgg cgcgccgccg
     3361 gaccaatcga gcgcgaagcg atcgggatgc gcgaaaacgt cagcgctgta gtagctgatc
     3421 ccaaaaggaa tgtcgcccat cagcgcgacc ccctgcccct cggcgtattt ttttacctcg
     3481 cgccattgcc gatgggcgat ccattgcacg tagttgtaaa attcccggcg ctcggcaaag
     3541 ggcgcgcgtt cctcttcgcc taacgagtca agccagcggt gggcggcatc gatctcggaa
     3601 tgttccgccc gccactcatc ccaggtttcg cggtccccgt tcctttccat caggacacgg
     3661 aagagggtgt aatcggcgag ccaggatttt tccgcttcgc agaaggaagc aaacgcctct
     3721 tttgtcgccg catcggtccg cgcgccagtg gcaaaagcct caaaggcgcg ctcgagaagc
     3781 tttttcttca acggtttgac gatcccgtat ttcacgcggc cgcgtcgcag tgccgcgagg
     3841 tcaattcccg cggtcacctc ggcaaaggcc tgcgccgaga gatcttccgg cacgcccggc
     3901 gcgagctcga tggtggtcgg gtcgatcgcg cgcgagctga ttgcattata cggactgtgg
     3961 tcgcctccgg tttcgttgat cggcaggagt tgcaccagtt tgaagccgat ggtgcgggcc
     4021 caggcgatga attgccgcaa accggcgacg tcgccgatgc cgagatcatt ctccgtccgc
     4081 agggcgaaga gcggggcgag cacgccggcg atcttttcgt taggcgaaat agtcatgcgg
     4141 gcaatgacga agcacgaatg acgaaattcg agtcaatctc gaaggtcgaa tgacgcaaga
     4201 gcatctcctg tcttcgtcat tttatcccct ccgcacattc cagtcttcga caagggagag
     4261 cggaatctct ttcaggaaac gcgaagggcg ctgaaacatg tcgccgtagc cggcgttgag
     4321 gcgcaggtgc ggataggtga gataaagttc gtctttggcg cgggtgatcg cgacgtaaaa
     4381 aagacggcgc tcttcttcga tcgcgtcgcg ggtctcgagc gagcgggtgc tggggaacat
     4441 tccgtcggtc agccagatga caaagacgga gtggaactcg agtcccttcg cctggtgcac
     4501 ggtggagaga ttcacggcct cggtgtcgcc ggtctgattg gtcgccggct ccgcatcgag
     4561 attgctgatg agcgcgagtt gcgagagaaa ttcgtgaatg tctttgaact gacgcgcgaa
     4621 agcggcgagt tgcgtgaggt cttccttacg cagctcgtag ttggcaaaat tggccttcgc
     4681 gtaatcatcg taaatcgcct cgacgatcga catgatcatc tcggtcggct ggttcggtcg
     4741 tccgcgcggt gcgatttcgt cgagagtcca ggcgagttgc tcccaggatt tctttgaccg
     4801 cgcgccgact ggcagcgcac gcaggcgctc gccgaagttg tagccgggat cggcgatccg
     4861 ggccgagatt tcggccccga cgctgccggg gtcggcaccc gcggtgacca cgggagttgc
     4921 ttcgcctaac gactcttccc aggaacgcca gagattctcc gcgctcttgc cgccgatccc
     4981 cggcagcagc cgaaccattc gtttaaaggc gacttcatcg cgtggattag cgacgaagcg
     5041 aaggaaggcc gccacgtctt taatgtgggc ctgctcgaag aaacgaatgc cgctcgtgat
     5101 ctggtaggga atgccgcgac gcgcaagctc caattgcagc tccaccgcgt gatagtgcgc
     5161 ccggtaaaga acggcgatct cgtgcaactc gatcccttca tcgcgcagct cgagaatgcg
     5221 ctgggcgatg aactgcgact gctcgccgcc atcgttcagg gccacgatcg cgggtttcaa
     5281 cgagttcgtt aggcgggtcg cgctgagctg cttccggaac tgctggagat tggccgcgat
     5341 ggccgcgttc gccacctgca ggatctccgg cacgctgcgg taattgagct cgatcttgaa
     5401 gacctgcgcg cccgggtaac gccgggggaa ggcaaggatg ttttggaagt tggccccgcg
     5461 ccaggaatag atcgattgcg catcgtcgcc caccaccatc acgttctggt gttccgccgc
     5521 gagcaggtcg ataaggtcgg cctggatctt gttcgtgtcc tgatattcgt ctaccaggat
     5581 gaactggaat tgtcgccgat agaaggccgc gatttcctcg tgctccttca gcatccggag
     5641 cgttttctcc agcagatcgt cgaaatcgag tgagttagtc gattttttcc gccgctcgta
     5701 gcggagatgc acttccttga tttgctcaag cagcggaagg aaatagggaa acttttccgc
     5761 cagcaattcc tcaatcggcc gctcggtatt gacgacgaaa ctgaaaatat ccgccagcac
     5821 ctcacccttc gggaaacgga tttccttcgg atcgatgccg gagcccgcga cgaccgagtt
     5881 gagcaggtct ttctggtcct cgcggtccat gatcgtgaat ccgctcgagt atccgagggc
     5941 gctgccgtgc cgccgcagca tccggttgcc gatggaatgg aacgttccgc cccagagccc
     6001 gcttgcatcg acggggagaa gattcgcgac ccggtcgagc atctggcgcg cggctttgtt
     6061 cgtgaaggtg aggagcagga tgttgcgcgg gtcgacgccg ttttcgagta ggtaggcgac
     6121 acgataggtc agggtgcgcg tcttgcccga gcccgcgcca gcgatcacga gcagcggtcc
     6181 gggcggcgcg gtcacggccg cgtactgctg ctcgttcaac tcgttcccgt aatcgatgtt
     6241 gatcgtcgcc gcggagggag cgcgctggag tgtgtattcg cgggacattg gggggttacg
     6301 ctggagtctg ggtcacggct cttcaacgaa aagtttcgcc tccggcaaac cgcgctctcg
     6361 cctaacggaa aaattcgtgg tggatcgagc gggccggccc tctgcgactg aacgcagggg
     6421 cctgaaacaa gaggctgacg ggggatctgg gccgtccggt ttgaggaaaa aaacaggggc
     6481 cggggacagt tggcaaggtt actgcttatt acgattatag taattatgga atcacccgcc
     6541 gcaccagctt tctcgctatg aaaatcctca accgggaccg ggccgaattc cttgtgattg
     6601 atgacgaccc ggcgattaca agattcctcg ccgcctacct gaaacagcag gggcacacct
     6661 gcgcgacttt gacggagggc ttccagaccg cctcctggct cgaactgcac gactgcgaag
     6721 tggtggtggt cgatctaaac atgccgaaag tggacggaat ctcgctcatt acctatgtgc
     6781 gcgagatcca gccgagcctg cccattatcg ttttcaccgg cgtcggctac gatgaggaaa
     6841 aaatgcacgc ggctctgcgg gcgggcgcga acggctacgt gagcaagaat ctcccgatcg
     6901 agcaacttta ctgtgtcctt tcgcgagtcc ttgcgacgtg ccgacagcgc gcgcgtcgtg
     6961 aggcgctcgc tcgggcgcgc cccgcgctcc tcggcgcagc ctaacgagga cgcgcttcct
     7021 gcaagagatc gcgtaatcct tcgggagaaa cggtctcggc gccactcgcg ctcgctgttt
     7081 cctgctcgag gtaatcgccg gtcgcgacga cgagatcgaa gcgctccgca tatttgctcg
     7141 tcaaccgctc gatgatcgag tcggccgttt gcccgtggcg cgaatagaag atttgaattt
     7201 cacccggctc ggcttcatgc gagacggccg tgccgcggcc gtcgaagacc acaaccacac
     7261 gcacgccgct ccaatcttgg tagtcgcgca actgtcggat gagagcctcg cgcgcgaggg
     7321 aggtgcggcg ctcatggagc agtcgcagtt ccggccaggc aaaaataatg ctatgaccgt
     7381 cgaccaagag atattggggt cgcacccgtc acgatagaat ggcggggtcg ctggaatcaa
     7441 cccgcgtctg ccgggcggcg ttcttaagag gcggcggcgg ccggcttcct ggcgcgaggc
     7501 tttgctccgg tccggcgcgg cgctttcgcc gccacccgct tgcgttcgac ataagctttc
     7561 cgccgctttc gcttgacggt gactcggtgt tgttgaccca tgaggcagtt gctataggcc
     7621 ggatgcgcca acggcgcaat cggaaccgtg cgtcggtgcc gcaaacaaat tgtgaattgg
     7681 atgttgccat gtcaggcggg tcccgtctaa aaagagtgcc tcaccctcgt ctcagcgcga
     7741 cgttacccca gccccttgcc ccacatgccc aggatattaa tcggagtttc cgccttcctg
     7801 atggcgctct ccctgttctt cgggttcttg aattcgagca agttaaaggg cttgcggaac
     7861 gaaagcgtca gcaccacctc ggcgcgcgag acagccgatc gggcccggat ggcgagcgag
     7921 aggaagttga aggcgcgcga ggtcgagttt gcgaaggcgg cgagcaaggc gacggcggcg
     7981 gaagcgaagg cgacgagtgc ggaaaccgac ctgagcaaga cgcaaaacga gaaagccgaa
     8041 ctaaagtcca aattacaggc gagtgagggc cagatcgcgg acctgcagaa gcgggtggcg
     8101 gaggcgacgg tcggcgaggt gccaggcgca tcggaaggcg tttcgcctaa cgaattgaaa
     8161 gcgttgctcg atgacaccaa gcaccaactg gtggctgcgg aacaggagaa atcgattctg
     8221 gcggacaagg tgagagccgc gcaggaccgg gtcgcggcca tcgaggagga gaaaaagcgg
     8281 cgcgaatcgg gggtcaacgc gcccggcgtt cacggcacaa ttctcgccgt caaccaggcc
     8341 tacaatttcg tcgtcctcag catgggcgat cgacagggcg tggtgccaaa ctcggagatg
     8401 ctggtcatgc ggcgcggggc gctgattggc aaaatccgcg tctcctcggt tgaaccaacc
     8461 acttcgatag gcgatatcat caccaatagc ttggcgcgcg gcgtccaagt gcagcctgga
     8521 gataccgtca tttatgctgg aagtaacaat tcgtcatcct agggtccgct cctgcctaac
     8581 gaccttgctt gcggcggccg ccttgagttc gtgtgccacc gatgaaccaa aacagaccgc
     8641 tttgctgggc gaccccgacg cgcggcgcga gtcgagtatc ccgtggaacc ggccgcaggg
     8701 ctgggaaaac accgccggct tgccctctgg actcaatggc tccggcggcc aacccaatcc
     8761 caacaatccc gctggcgatt gaccgtcccc gccggaagga cgcgatcggc tagatccaaa
     8821 agccggcggg aatgaccgca tccttcggca ccaccacgat cccgtcgcga atgtagtaat
     8881 tgggcgcgtc gagattcgcc tctttgcctt ccggcgtgat caccacgccg tcgccgatgc
     8941 gcgcgttttt gtcgatgatc gcgcgatcga tcacgcaatt gcgcccgatg ccgatcggcg
     9001 gcagatcggg cgatttggca ggcgcggctc cttcgaaatg gtcgccgccc atgagcacgc
     9061 tgttgcggat cgtcgccccg ctccggatga tgctgcgcac cccgatcacg ctccgctcga
     9121 tgtgggcgtc ggagataatg caaccatcgg aaatgatcgc ctggcgcagc gtcgcgccgt
     9181 tgattttgct cccgggcagg aagcgcgggt gggtatagat cggcgcctgc gcttcgaaga
     9241 agctatagtt aggcgcgaga ttggtcaggt cgagatttgc ttcgtagaag gcgcggatcg
     9301 ttccgatgtc ttcccagtag cctttgaaaa tataggcatt gacgtggcgt tcgccgatgg
     9361 cccgcgggat gacgtgcttg ccgaaatcgt cgaggtcgct gttcaggcaa tcgaccagca
     9421 cctggcgatt gaaaacatat atgcccatcg acgcttgaaa cagttcttca tcggggtcgc
     9481 tctcgatcga attgagcagc tcgcggtcca tcttcagctc gtcgagcacc gctgtttcct
     9541 tcggcttttc ctcgaagcgc gtgattctcc ggtccggtcc gctcaacatg atcccgaaat
     9601 cggaggcggc ggcgcgctgt accggcgtcg tggccagcgt gatctcggcg ccggagcgga
     9661 tgtgctggtg caagagcgtc cggaaatcca tccgatagag ctgatcgccg ctcaggatga
     9721 ggaaatagtc gtagggctgc tcgagaaaat aacggagatt ttgccgaacc gcatcggcgg
     9781 tgccctgata ccattgagcc ccttcggggg tttgttgcgc ggcgagaatg tcgacaaagc
     9841 tgcgcgaaaa attatcgaac ttgtagctcg cctggatatg gcgatggagc gacatgctgt
     9901 tgaactgggt gagcacgtaa atcgagcgca gcccggagtt gaggcagttg ctgatcggga
     9961 tgtcgaccag ccggtacttt cctcctaacg gaacggctgg cttggaacga tccttggtca
    10021 gaggaaacaa gcgcgtcccc gcgccgcccc ccatgatgat ggagagggtg cgttgggtgg
    10081 tgccgggcgg cagagcagga gattgcggca tgcgaaaagt tcaacttaca cccgatcgaa
    10141 ctccattagg caagccgttc tactccgctt tcttcccaaa tcctgctccc gctcttgctc
    10201 gtgatccttg cggtcaggag caagagcagg gttggaaact cggctaccct acgccggcac
    10261 gccttgcgtc cggcgagcgt cggtgtcacg cttccgccct aatttatgtg tggcatcgtc
    10321 ggatacgtcg gaagagcgga agccgcgccc atcctgctcg atggcctgcg ccggctcgag
    10381 taccgcggct atgactccgc cggggttgcg atcgtcgatc gcgatcacgt cgagacgcgc
    10441 aaatgcgccg gccgcatcgc cgggctttcg caattaatcg ccgaacatcc cccttccggg
    10501 acctacggca tcagtcacac ccgctgggca acgcacggca aaccgaccga tcaaaatgcc
    10561 catccgcact tcgacgagag cggcaaactc gcgctcgtcc acaacggcgt gatcgaaaac
    10621 taccagcaga tcaaggacgc gctgatccag gaaagcccgc atcactttac atccgagacc
    10681 gacacggaag tgctggctca cctggtcgga aaaatttacg atgactccgc ggttggagtg
    10741 gtcgatccgc gccggcacaa ggcgttgctg gtggaagcgc tgcgcacggc cttgcaacag
    10801 gtcattggca cctacggcat cgcgctggtc cacgcggata ttccggattt catcgttggc
    10861 gcgcgccgtg gcagcccgct tgttctcggg gtggggaagg acgagaattt tctggcgagc
    10921 gatgtgagcg cgatcgtcgc ccatacccgc gacgcggttt acctaaacga cttcgatctg
    10981 gtcgcggtcg agcgcgacaa cttcgagatc agctcgctcg ccggcgagcg cggcagccac
    11041 gcgatcagca aggtcgaatt caccgccgag gacatcaaga aaggcgatta cccgcactac
    11101 atgctcaagg aaatcttcga gcaacccggc tcggtgcgcg acgcgatgcg gggccggctg
    11161 agcgtcgaag aatgcaccgc gaaactcggc ggcctgaaca tgaccccggc gcaactgcgc
    11221 gacgtcggcc gggtggtttt gaccggctgc gggacggcgc tgcacgccgg catggtcggc
    11281 gaatacctga tcgaacagct cgcgcacatc ccggtggagg tggaatacgc gagcgaattc
    11341 cgccatcgca acacgccgat gacgaccgac acgctggtct tcgccatcag ccagagcggc
    11401 gagactgccg acacgctagg cgcgctccgc gaaagcaagc gcaaaggcca tcgcacgctg
    11461 ggcatttgca acaacgtcgc gagcacgatc gcgcgggaaa gcgacggcgg cgtctacatg
    11521 cacgccggac cggaaatagg ggtcgcggcg accaaatcgt tcacctcgca ggtcgtcatt
    11581 ctcaccttga tcggattatt gttaggccgg atgcggcatc tctcgaccgc ggaaggcact
    11641 tccatcagcg gagcccttga ggcgttgccg gaaaagatcg agcagatctt gaaaatgagc
    11701 gaccaaatca gggaggtcgc aaaaaaatac gcccaggcga cgggcatgtt gttcttcggc
    11761 cggcagttca atttcccgat cgcgcgcgaa gcggcgctca agttgaagga gatcacctat
    11821 ctctacgccg agggtcatcc gagcgcggaa ctgaagcacg gcgtgatcgc gctcgtgaaa
    11881 ccggagctgc cttccgtttt catcaccccg gacgacgcgg tcttttccaa gaacatgaac
    11941 aacatcgagc aggtgcgcgc gcgcaaaggg ccgatcatcg cggtcacgac tgcgagcggc
    12001 gcggcaaaat tgaaaggcat cgccgacgac atcatcaccg tgcccgacgc ggatgagatc
    12061 gtgatgccgg tgctgaccgt cattccgctg caattatttg cctaccatct cgccgtcgcc
    12121 cttggccgcg acgtcgacaa accgaggaat ctggcgaaaa gcgtgaccgt cgagtaacgg
    12181 cacgctgttg caggctgtag ccgcttcgct gtgcgaagcg cgggtatcga cgccgttcct
    12241 gcgagagtcc ctcccattgc caaccgcatg cccacgtcgc ccacagcgcg gcggctacgg
    12301 ccgggcgaca acagtttaag acggccccgc gcctttgacg ccgacccgcg ccagatattc
    12361 ctccacgctc aggagattat tggcgccgcg ctgcacgatt ttgaggagcg tatccatcga
    12421 ggaaacttcc tcgagctgct cggtcaggaa ccattgcaga aaattattcg tgatgaaatc
    12481 gttctccgag cgcgccaatt ctaccagcgc gttgatctgg cgcgtgacca cgatttcctg
    12541 gtcgagtgag agccggatcg cttcctccgc gttcttgaag gtgggctgcg gcgcggcgat
    12601 cgcgggaatc accacccggc cgccggcatc gaccacatat ttgatgaagc gcatcgcatg
    12661 atcattttct tcggccgcct ggcgataaaa atgctccgcc agctgcggga gggcgtcgcc
    12721 cgaaaagtag gccgcgatcg cgacatactg cagggacgcc ccaaactcgt ttccgatctg
    12781 cttgttgatc gcgttgacca ctttcttgct tgtgagcatg ggaaccttct ccagttaaga
    12841 tttgttcgcg gtgccgctcg gcggacgaaa cgcccaagcg acggtggtgc aacatggacg
    12901 aagaatcctt cgttgccaat ttggaaccgg ggcgggacaa ttcaccgttt tccagccatt
    12961 cgttaggaga tcaactgcgt ttgccggcga tgatccggta gatgagcaga agcagcatcg
    13021 cgccgatgat cgatgcgatg aatcccgccg gctgccccgg ttggtaccag ccgagcacac
    13081 ggccgagata gccggccaca aaagcgcccg cgacgcccag caggatcgtg atgatgcagc
    13141 cgccgggatc cttccccggc atgaggagtt tggcgatcgc gccaacgacg agaccgatga
    13201 acagtgtcca gagaattgcg cccatgattg aggttaagaa tcgcgggccg caggggcaac
    13261 ggttttatgc tgctacaact ccaccacctg ggcgctctgg atatcgtcgc tcgccgcgat
    13321 ctcgacgagc aattcgggcg gcggcgcggt gtcgagattg agcacggtga gcgcgcgtcc
    13381 gccggcctgg tttcggctga gcgacatcgt cgcgatgttg acgtcgtgcg cgccgaggag
    13441 agtgccaatg cgcccgacca tgccgggacg gtcggtgttt tcgagaacca gcagaacgcc
    13501 gtgcggacgc gcttccaccg gccggctgtt gatgctcacg atccgcgggg tcgcgccgaa
    13561 gaatgcgccg gcgacggaga gcgtttttcc ttccgcctcc gccgtcagct cgagcagatc
    13621 ggtgtaatcg cccggcgcgc tgaggcggga ttcggtcacc ttcatgccta acgtttcggc
    13681 gaacgcgggc gcgttgactt cattcacgtc ctcgccgccg gcgaggtgga gaaagccttt
    13741 caggatcgaa cgcgtgatcg ccgtcgtgtc gacctcgttc actttgccgc tgtagttgat
    13801 atgcagattt tcgactcgct tcggcgacac ctgcgacaag aagcgcccga gcttttcgcc
    13861 aaaacgcaaa tggggcccga cgatggcgag cgttttcgcg tcgagattcg gcagattgat
    13921 cgcgttgcgg atcgtcccct cgaggagtgc ggcgcgaatc gcctgtgcga tttcgatccc
    13981 gacactttcc tgcgcctcga ccgtcgaagc gccgagatgc ggggtgagga tcaggttcgg
    14041 aatcccgcgc agcggcgaat cagccggcaa cggttcctgc tcgaaaacat cgagcgccgc
    14101 gcctgccacc tggccgttgc ctaacgattc gaggagcgct acctcgtcga tcaggccgcc
    14161 gcgcgcgcaa ttgatcaggc gcacgccgcg tttcgtcttc gtgaggcgcg cggcgttcag
    14221 gagatgatgt gtctctggcg tgagcggcgt gtgcagagtg agaaaatccg ccagcggcag
    14281 gagctcatcg agttcctcga ccagttccac ctggagcgat cgcgcccggc tcgaggagag
    14341 atacggatcg taggccagca cccgcattcc gaaggcgatc gcgcgccggc tcaactccga
    14401 accgatccgg cccatgccga taatgcccag cgttttgttg taaagctcgg tgccttcaaa
    14461 ctgcttccgg tcccatttgc cggcttgcag attggaatgc gcctgcggga tcttgcgcgc
    14521 cacggaaacg aggagcgaga aagcgtgttc cgcggtcgag atggtgttgc ctccgggcgt
    14581 gttcatcaca atcactccgc gccgtgtcgc ggcctcgaca tccacgttgt cgactccgac
    14641 gccggcgcga ccgaccacgc gcaagcactt acccgcctgt aaaatcgggg ccgtcacctt
    14701 tgtccggctg cgcaccacga gtgcgctgaa ctcgggaatc aactgcacca attccgcctc
    14761 gctcaagccg gtgtgcacgg tgacgtccag cgccgcgccg gccgcgagct catcgattcc
    14821 tctctgagaa atcgaatccg cgattaagac cttgggcgcg ctcacacgta aaggtggaga
    14881 cgatctaacc aaatatcaca agtcgtattg tgtcgggtaa ttccgccgcg accctggtga
    14941 ccaggctccc gcccaacagc tgtgcgagcg tgctgcgttg cgacgtgccg atgatcaggt
    15001 aatcgacacc catcgtcgcc gacagatcga ggatggtcgc ggcggcatct tcgctcaccg
    15061 cgtagaccgg caacacacaa acgccgcggt cctgcccctg tttcagcatc gacgacatga
    15121 tcgcctgcgc aaccgggtcg tcctgccatc tcacccgccc gagagttgcc ggccctccgg
    15181 agaaatagac ggcgatttct cgcacgtaaa gaacgcagag taccgccttg cgcagctccg
    15241 cctcttcgag ggcaaaattc aggaccggcg tgacgccgcg tgctgcgacc atgattttgg
    15301 tgccttcctc aagccgcggt gcaaacgccg caagggtttg cggcgacacc atatcggcga
    15361 cctccctggt cacggtcagt gtcgttaggc cctggcgctt gagcgtgtag gcgcgcaagg
    15421 ccaaaccgcc gaggaggacg cacatgacga agaaaagtgc atccggtttg ctgtgcgcca
    15481 acgtcagctc aaccagaaaa agaatgacga acgtgatccc gaaaagcacg cggtcgtacc
    15541 aggtaaaccc ggctgttcga ttgagagcgc aagagccgac attgaccgca atcgctccca
    15601 ccaccccaat cgcgtagagc ccggctagtg aggtaaaccc ggttgccgtc agcaatacca
    15661 gtgagggaat gccgactgcg atgaagagag gatagatcgg gacgccatac ttattcagcc
    15721 tcgtgaattg tcgcggcatt tcgccgtcgc gcgccatcat gaaaagcagg ccgatcatcg
    15781 cgacgatggc ggtattcgcc gcactcacca ggaggaagaa gaaaacaatc ccgacgaccc
    15841 agccgaacat gacgccgaac catggagaga cggtcgccgc gccgaactgc tcaccgataa
    15901 agcggagcat gtcttcgttg cgcgctccca ttgccgcccg aatctggagc ttgtctgtga
    15961 ggtgcagcgt ctggggaaga acgctcggca acgagagcat cgcccatccg agcaaggcgg
    16021 tgccggcaac gacttcaatt gccaccggca ggatcgcctg caacgaggtg cgcgcgacgc
    16081 ttggcttatc catcgtcgaa ccgcgatcca gcttcatgac tccggtcagg ttggcgatcg
    16141 actccgcgcc ggagagtgcg agaattacgc cgacgaactg cacccaaagc gcggagaaat
    16201 gttggtgtcg cggttcgaga aattgcgtcg ttagatgcgg tgcgctgacg ccgatcagca
    16261 ccaaaacaac gatggccgtc ggcacggaca ggacgacggc cagacttccg ctgtgctttg
    16321 gcccaaaata attgagccag cccataataa tcaacaccgc gatcgtggtc agcccgatgt
    16381 gatcgcgcag cattttgatc cagacgacct gttccgcgcc ggaagtgatg taactcaagc
    16441 cggcccagcc gctaagcgcc gccgtcaccg tcagatctgc cagcaaaagc agggcgccca
    16501 ccacggcgag cagtcggccc tgtgatcggg ccgccgaata aacgccgccg ccgtcgggga
    16561 aatggcggca gatgacgatg taattgacac ccaccagggc cgttaccacg cacaccgcga
    16621 gaatgatcgg caatgaagaa aaccccgcgg cgagaaaagc gaggccgatg acgtaagcct
    16681 tgctcgtgcc ccaatcgccg taaagcaaag cggcggcgcg tttccagtca acgttacggg
    16741 ggcgatgcac gccgggagtt gcgatgaacg gttcagccat gaaaagtgcg agggggagtt
    16801 accgacgcat cgccgcggcg ggcaagtaat ttctcggcaa cgccggagaa cgcgacggcg
    16861 gcatccgtgc aggcatagag cgatcggttg acattgatgt atcgccgtat caatgtatca
    16921 acgtatgcga accacgatta ccctcgatag agatgtcttt gaagcagccc gaaccctggc
    16981 cgcgtcctcc ggcaagaagc ttggccaggt tgtttcgcaa ttggcgcggc gcggacttcg
    17041 tgccccgccc gacgtggccg ccaagaatgg tttgccggtc tttcgcgtct cgcccgacgc
    17101 cccggtgatt ccgagcgatc gtgcgcgcga tttgctggcc gatgaagaga catgaagccg
    17161 gcgctcctcg ataccaacgt ccttctggcc ctggcctggc caaatcatca gcaccacgcc
    17221 gaaacgcacc agtggtttgg agcgcaatcg cggcatgggt gggcgacatg cgcgctcacc
    17281 cagctggggt tcatccgtct ctcggccaac ccggcttata cacccgccgc ggtttcaccg
    17341 caggaggctg cctccttact cgaacgcctg cttcggcatg agtcgcacca tttctggcca
    17401 tgcccggcgg cgacggcgcc tgcgatttac tctcgcacgc tcggccatca acagaccaac
    17461 gatgcttatc tcgtcgaggt ggttcgccgg cacaaaggtc ggctcgtcac tcttgaccga
    17521 cgcgcctcgg cccacggaga cgaagttgtt tttgtgattg ccggttagtc tgtcgccaag
    17581 tttgctggcc cttcgatctc ggcggtctgc gggaacatgt cgcgcaaatt ttctggcttt
    17641 tgtagtgctg cgtcgacgtc cgtgagatca ccttcagatc agcgatcttt gatccgtgtc
    17701 aagaaactgc tgctccttcg ggggcagtac cattcgaatg ctgcccgcga cgaaggaatc
    17761 tgcatcggcc acttcgaaat cacctgcgat agcaggggcc gggcggggcg tgcccaagcg
    17821 gatgaagtaa agggccaggc ggttatgagg catttggcat gacctcatct ctccagtccc
    17881 acactccgac tgtgtggcgt gggaacctcg cctaactacg tggaactgga ttttacacag
    17941 cggaatggct gctgccggtt acttgccttc tatcggtagc aggttgcggc gcgagcattg
    18001 ccgtccttcc gtttgaaaac ctgagtagcg ataaagaaaa tgcctgcttt gccgacggca
    18061 tccaggctga agtcctgacg cggcgatcct gtctgcgtct tatagggggt cgagatcgtc
    18121 gtcaagggta actactttga agaggcggtt ctgaggaacg accgtcaggg atcgttcccc
    18181 gagccgcttt cgcaacccgg cattgacatt caaatcctcg cgtgcgcgcg tggaggcgcg
    18241 tggaggtttg acgcctcgcg ccggagcggt aggcttaagc gtgatcttca cgatcggacg
    18301 tgcagctacg atcctcgcac tttgcctttc gttaggcctg cattgggtgg ctctccagtc
    18361 ggtcgcctgg acaacgatgc tggtggagaa tgcctgccat gtgccgctca gtgacgcggt
    18421 ggccaagact ttcgacggca atcatccctg cgacctttgc cacatggtcg ccaccggcca
    18481 gaaatcggag aaaaaatccg agatgctgcc gacggttggg aaaatggatc tcatttgcac
    18541 gacgcgcacc ctgagctggc tgccgccgtg ggtgtcctac gactatccgc ggatcaactc
    18601 cgcgattccg gagcgctcgc aagcgccgcc agtgcctcct cctcgcttgc tgccgggttg
    18661 attcctgctc gttaggcgga ctgcgccgcg cgtgtgcgcg ggtcgcggtt cctttctcgc
    18721 aacccgggaa cttttcgccg tggcgcgaac gtttccacat caccttggag gtataactca
    18781 tgaaacactt tgtcttactt ttttggctta cctgcgccgc gtgcgagttc ggcgcgacga
    18841 gccgggcgta cgggcatggc ttcgcgggcg cgcggttttt tccggcgacg ctctccaccg
    18901 acgacccctt tgtcgccgat gagttttcgc tgccaactgt ttccagcatt attacaccgg
    18961 acaatggcgg cacgcgggat acggagattt cttccgacat cgctttgcgc atcacgccga
    19021 aatgggacat cgagtttggc gagacattca ttacgctcaa tccttcacag ggcaaggtgg
    19081 tgaatggttt cgacaatctt tccatcggct cgaaatgcga gttctttgaa aacgacgaac
    19141 acgaaaccgt agcgtccctc ggcctcgacg tcgatgtcgg cgggacggga agcaagcaat
    19201 tgggagcaga tactttcagc acgtggacgc cgggactctt ctttggcaaa ggcttgggcg
    19261 atttgccgca aggattgtcg ttggtgagac cagttgcggt caccggcctc gtcggcgtcg
    19321 ccgtgccggg cagggcgagt acggacggat caccaaaccc tgctgttttg caattgggtt
    19381 ttgcgtttga atacagcatt atttacttgc aggagcaggt cagagacatc gggctgcgcg
    19441 cgccgttcga ccgcttgatt ccgctggtgg aagtttccct tagtgatcca ctcgaccgcg
    19501 gggagagcgg cctaattacc ggcacggtca atcccggcgt catctggtct ggcaaatact
    19561 tccaagtcgg ggccgaagcg gtgattccca tcaattcgcg cactggcaat aacatcggat
    19621 tcattgcgca gctgcacttc tatctggacg atcttttccc gcacagcctg ggccgcccgc
    19681 tcttcggagg aaacaaatga aacgtatcat cctcagtttc gttctaattg ttctggccgg
    19741 ttcagcctcc cttgaagcgc atgcgttcct caaaggtgcg gaaccgagcg tgggcagcac
    19801 ggtccagaca gcgccgaacg aggtgcgaat ccggtttacg gaaaacatcg aacccgcctt
    19861 cagtaagatt caggtcttca acctgtccgg gaacgaagta gacaagcgcg atgtgcatct
    19921 ggatcgctcc gatcccgcgg tcttgcaggt ttcattgtcg ccgctcggaa gcggaaccta
    19981 caaggtggtc tggcgtgtgg tctcggtcga tacacatgtg acggaaggaa gcttcagttt
    20041 ccatgttcgt taggccggaa atccgtcggg aaacctgatg cttgacgctt tgctcatcgt
    20101 cgcgcgcagc gcgcagatcg cggcgtcgat cctgttcgcg ggagttttta cctttaaggt
    20161 ggtcgcgctc gggcgcgtcg ggcagtccgc cggcgatgcc gggcaggcgc tggatcggca
    20221 gttcttttgg ctcgcccttt ggagtctggt cgcggcgctt ttctccgcgc tcctctggtt
    20281 ttggctggtg gtcgcgagca tgagcgggtc gtccctggcc gatgctcttt ccggaaacgc
    20341 ctggcagatg gtgctgtcgc aaacggagtt cggccgtgtc tggcagctgc gcttggcagt
    20401 gatcgggctg gcactcgcgc tcgtcgcggt cggcttgccc cgaaagaact cgcgccatgc
    20461 gttgacgctc gccctctggc cgttggcgct ggtattgctc gtctcacttg cctggatcag
    20521 tcacgccgcc gctgccagat cccaaccgct cggtctgctc ggcgacgcgc tccatctttg
    20581 cgccgccggc ggttggattg gcgggttgtg gccgctcgcg attttcctga aacgcaccgg
    20641 ggtctcagtc tcgtccggcg cacctgcgct ccatgtcttg cgtcgctttt ccacgcttag
    20701 cctttgctct gtcggcgtcc tcgtggttag cggcctttcc aatagctggc ttctggtcgg
    20761 ctccgtcgca gcgcttttca cgacccgcta tggcgcgctt ctcctcttca agctgacgct
    20821 ttttgccctc ctcctcggct tcggcgctcg gaaccggggg atcatcaaca cggccgcgat
    20881 ctctccgggt ttgctggcgc aactccgccg caacgtgctc tacgaaatct gtcttggcct
    20941 ggcggtagtg gcgattgtgg gatggttagg tatcaccccg ccggcccgtt ccgggacctc
    21001 ggccgaaaac aggacgttgg cgatttcttc ttcaaggtaa aaagcggatt ctggaaaatc
    21061 ggcgatcatc gctgcagaaa actcttgcgc gctgttcggt tcgatcacaa cgtagctctc
    21121 gccgtgagcc gccgcgaaaa actccaggcc tttgtcgcaa gcggattgct ttcggccttt
    21181 ctcttcgcgc tcgcgctcgc tgcttcgccg caactccacc agcggttgca tgccgacgct
    21241 ggttcgccgg atcacgagtg cgcggcgacg ctcatcgctg ccggcaaata cgagcaggcc
    21301 gatgccccgc ccgtcgtggt cgcgccgcag ccggcgcttg tttttgagag aattccg
//
LOCUS       DATMNS010000220         4458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1029410, whole genome shotgun sequence.
ACCESSION   DATMNS010000220 DATMNS010000000
VERSION     DATMNS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1029410"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(867..2114)
                     /gene="fabF"
                     /locus_tag="VNW28_07750"
     CDS             complement(867..2114)
                     /gene="fabF"
                     /locus_tag="VNW28_07750"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HXA09862.1"
                     /translation="MNRRVVITGMGVVTPLGNELEVFWKNLLEGRSGIVEISRFDTTG
                     YDCRIAGEIRDFDPRTVFNNPKEHKRTDRFSQLAMGATKNAMRDSGIDLEKIDRDRFG
                     VLVSSGIGGLKTLEDQHTALVTKGPSRVSAFTIPMLISNMASGLISMEFGLRGPNMCI
                     VTACATSNNAIGEAWRIIKFGDADIFLAGGAEAAIIPIGLGGFSAMKALSTRNDEPQR
                     ASRPFDRDRDGFVMGEGAGVMVVEELEHAKARGAKIWCELSGYGLSGDAYHMTAPPPD
                     GEGAARAMKMALGHASLNPADVDYVNAHATSTGLGDICETRAIKTVFGDYAKNGLAIS
                     STKSMTGHLLGGAGAIEMAACAMAIRDGRIPPTINLENPDPECDLDYTANVAREKRVR
                     VAVNNSFGFGGHNATLVVTEFKD"
     gene            complement(2211..3383)
                     /locus_tag="VNW28_07755"
     CDS             complement(2211..3383)
                     /locus_tag="VNW28_07755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HXA09863.1"
                     /translation="MWIGRNRKARVTYGFDEIALVPGRVTINPNEVDITWGLPRRNAE
                     PLEFKIPILASAMDGVVDVRFAIEMGELGGLAVLNLEGVQTRYKNPEEVLQKIIEADK
                     SEITALLQRIYQEPVQEDLIAARIREIKDAGVPVAVSSIPQRAESFGALAAEAGADVF
                     VVQSTVSTVRHISSEYKSLDLDRFCHELRIPVIVGNTVGFDATFEIMECGPAAVLIGV
                     GPGAACTSRGVLGLGVPQVTATVDCAAARDAYFKKTARYVPIITDGGMSKGGDVCKAL
                     ACGADAVMVGSAFAKALEAPGRGYHWGMATPHANLPRGTRIKVGATGTLRQILFGPAE
                     VDDGSQNLVGAITTCMGNVGASSLPEFQQTEIIIAPSIKTEGKLFQTVQSVGMGTR"
     gene            complement(3629..>4458)
                     /locus_tag="VNW28_07760"
     CDS             complement(3629..>4458)
                     /locus_tag="VNW28_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09864.1"
                     /translation="GARNYFRRARHHSPTVSAQSSAQPHGSLLFRWERPRPRRFAIAG
                     FLLASVGLHALCFYLFVVVYPPAISLLPPPAQVSVIAPTSSEARTFLNWLNAEDPALA
                     SQTQRPADARAFQLPKLQHVPSYLAVPPQLKELPPRQFTTSTPSGMPPAPVPRPPARN
                     PAPPAPAPTTLSFSDALRDRAVSPPDLRFRASLREAPESARFRVAVDSLGVVRYSFLE
                     QSSGDAALDEQARHDLVLCRFQRAAERNSGDGLVWATAWFEFGTDLELPPGATERAP"
BASE COUNT          841 a         1364 c         1412 g          841 t
ORIGIN      
        1 cccgagcctc caccgacgca gcctggttac cactgagcgt tttctcgaaa acggcatctt
       61 cggcctgtcg ccgcgcaatg gcccttgccc gctttggcgt ctgcgtagtt tcgggtacca
      121 atcaacccgc gatttcatgg cccgtctcag acccgcgtga gaattggctg cacttttggg
      181 ggcacgcgtg cgcggccatc tgggggcgca cgcgtgcccc caaaaagtgt agccaattct
      241 tttgttacgt tgccctggat cggcgatttg gaagtctgga agccgataag tcggacaagt
      301 tggctacgaa ctttcagccc gcgtcgcccg cagagcgaag tctacagggg cagatagcgt
      361 ggtggagttt ggcgcctccg actttgtggc gcgctgtaga cgcttcgctg tgcgaagcgc
      421 gggaggaagc ttcgctcgaa cgaaagattt tcccgtagtg aaacgcagac ccgcgtcgcc
      481 cacagggcga agtctacagg gaggtagcgg cgacctctca gtctttgaac tcggtcacga
      541 cgagggtggc gttgtggccc cccttcgaaa ccgaagcgcc gtggctaacg actcggacca
      601 cgtcggggtt tcgaaccgtt ccgagttgtc tcaacggccg taattaacac gacatcaacc
      661 gcagtgcgat catcgtcttt cttccacacg tatgcgatcc ggcctgaagg cgaaacggaa
      721 agaaaatcgg gatgcggaac gtgcaatttc tccgaccccg ccaacacgat attaaagggc
      781 tcaaagggag ccgcgtgcca aaattttctc aactcggtcg ccgtcataac gaaatcatcc
      841 cgtcccgcgc actcgcggtc aatgcctcag tctttgaact cggtcacgac gagggtggca
      901 ttgtggccgc cgaagccgaa tgagttgttc accgcgacac gcactctttt ctctcgcgcc
      961 acgttcgccg tgtaatcgag gtcgcattcc ggatcgggat tctcgaggtt tatcgtgggt
     1021 ggaattctgc cgtcccgaat cgccatcgcg cacgctgcca tttcgatggc gcccgccccg
     1081 cctaacaagt ggccggtcat cgatttggtc gaactgatgg cgaggccatt cttcgcataa
     1141 tcgccgaaga ccgtcttgat cgcgcgcgtc tcgcaaatat cgcccagccc ggtcgaagtg
     1201 gcgtgggcat tgacgtagtc gacgtccgcc ggattgaggc tcgcgtggcc gagcgccatc
     1261 ttcatcgcgc gcgccgcgcc ctcgccatcg ggtggtggcg ctgtcatgtg ataggcgtca
     1321 ccggagaggc cgtagccgga aagttcgcac cagatcttcg cgccgcgcgc tttggcgtgt
     1381 tcgagttcct cgaccaccat cacgccggcg ccttcgccca tgacgaatcc gtcgcgatcg
     1441 cgatcgaacg gtcgcgaggc acgctgcggc tcgtcgttgc gcgtgctgag ggccttcatg
     1501 gcggagaatc cgcccagacc gatcggaata atggcggcct ccgcaccgcc ggcgaggaag
     1561 atgtcggcat caccgaattt gatgatgcgc cacgcttcgc cgatcgcgtt gttggaagtc
     1621 gcgcacgcgg tgacgatgca catgttcggt ccgcgcaaac caaattccat cgaaatcaga
     1681 ccgctcgcca tgttgctgat gagcatcgga atggtgaacg cggaaacgcg cgacgggcct
     1741 ttggtgacca gcgcggtgtg ctggtcctcg agggttttca atccgccgat cccgctgctg
     1801 acgaggacgc cgaagcgatc gcgatcgatt ttttccagat cgatgccgga atcgcgcatc
     1861 gcgtttttcg tcgcgcccat ggcgagctgc gagaagcgat cggtgcgctt gtgctccttg
     1921 ggattgttga aaaccgtcct cggatcaaaa tcgcgaatct cgccggcgat gcggcaatcg
     1981 tagccggtgg tgtcgaagcg cgagatctcg acgatgccgc tgcggccttc gaggaggttt
     2041 ttccaaaaga cttcgagctc gttgcccaac ggagtgacaa cgcccattcc ggtgatgacg
     2101 actcggcgat tcatgacgaa gtgatgagtg acaagtgatg cgtggcgagc ggggtggacg
     2161 cgacgatttc agttacctgt cactcatcac ttggcacttg tcactctccg tcaccgagtg
     2221 cccatcccga cgctctggac ggtctggaag agtttgccct ccgtcttgat cgaaggcgcg
     2281 atgatgattt cggtctgttg gaattccgga agggaactgg cgccgacgtt gcccatgcag
     2341 gtggtgatgg cgccgaccag gttctgactg ccatcgtcca cttcggccgg gccgaacagg
     2401 atttgccgca gcgtgccggt cgcgccgacc ttgatgcgcg tgccgcgcgg gaggttggcg
     2461 tgcggcgtgg ccatgcccca gtggtagccc cggccgggcg cttccagcgc tttggcgaag
     2521 gcgctgccga ccatcacggc atccgcgccg caggccagcg ccttgcagac gtcgcctcct
     2581 ttgctcatcc cgccgtcggt gatgatcggc acgtagcgcg cggttttttt gaaataggca
     2641 tcgcgcgccg cggcgcaatc gacggtcgcc gtcacttgcg ggacgccgag gccaagcacg
     2701 ccgcgcgagg tgcaagccgc acccggaccg acgccgatga ggacggcagc cgggccgcat
     2761 tccatgattt caaaggtggc gtcaaaaccg accgtgttgc cgacgatgac cggaatccgc
     2821 agctcgtggc agaatcgatc gaggtcgagc gatttatatt ccgaagaaat atgccgcacc
     2881 gtggaaacgg tgctttgcac gacaaacaca tcggcgcccg cctccgcggc gagcgcgccg
     2941 aaactctccg cgcgttgcgg aatcgagctg accgccaccg gaacgcccgc gtcttttatt
     3001 tcgcgaatcc gcgccgcgat cagatcttcc tggacgggct cctggtagat tcgttgcagg
     3061 agcgcggtga tctcggattt gtcggcctcg atgatttttt gcagcacttc ctccgggttc
     3121 ttgtagcggg tctgcacccc ttcgaggttg agcacggcga ggccgccgag ttcgcccatc
     3181 tcgatcgcga accgcacatc gaccacgcca tccatcgcgc tggccaggat cggaatcttg
     3241 aattcgagcg gctcggcatt ccgccgcggc aggccccagg tgatgtccac ttcgttcggg
     3301 ttgatcgtga cgcgacccgg gacgagcgca atctcgtcga agccataggt cacgcgcgct
     3361 tttcggttcc ggccaatcca cattcccatg atttaaatcc gatccagttt aaaacgttcg
     3421 ttcaggctgc cgcagcgcgg acaacgcggc agaagcgtcg gcgtccgatc ctcgaactcg
     3481 caagcgcaaa ttacgcaccg cagccggtag cgcagcgccc gctgctcacg gcgtcgacga
     3541 ttccactcgc caaatatcca gaggagaaaa accgccgcaa gaaaaagcag cagataaatc
     3601 gtgaccagcg ccgcgaggct gacccgaatc acggcgcacg ctccgtcgcg cccggcggca
     3661 attcgagatc ggtgccgaat tcgaaccagg cggttgccca gacgaggccg tcgccggaat
     3721 ttctctccgc ggctctctgg aaacggcaga gcacgagatc atgtcgtgcc tgctcatcga
     3781 gggcggcatc gccggaggat tgttccagaa aactgtagcg gacgacgcct aacgaatcga
     3841 ccgcgactcg aaagcgcgcg ctctcggggg cttcgcggag cgacgcgcga aaccttaaat
     3901 cgggaggcga gaccgctcgg tcgcgcagcg cgtcggaaaa agacagggtg gtaggcgcgg
     3961 gcgcgggcgg ggccgggttc ctcgccggcg gcctcgggac cggcgccggc ggcatccccg
     4021 atggagtgga agtggtgaac tggcgcggtg gcaattcctt caactgcgga gggacggcga
     4081 gataggacgg aacgtgctgg agttttggca actggaaggc gcgcgcgtcg gctgggcgct
     4141 gggtttgcga agcgagcgcc ggatcctccg cgttcagcca attcagaaag gtccgcgcct
     4201 cgctggaggt cggcgcgatc acgctcacct gcgcaggtgg ggggaggagc gaaatcgccg
     4261 gcgggtaaac caccacgaag agatagaaac aaagcgcgtg cagcccgacg gaagcgagaa
     4321 gaaatcccgc aatcgcgaac cggcgcgggc gcgggcgttc ccaccggaag agcaaactcc
     4381 cgtgcggttg cgcggagctt tgcgcgctca cggttgggga gtggtggcga gcgcgacgga
     4441 agtaattccg cgctccag
//
LOCUS       DATMNS010000221         3674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3023491, whole genome shotgun sequence.
ACCESSION   DATMNS010000221 DATMNS010000000
VERSION     DATMNS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3674
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3023491"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1146
                     /gene="hflK"
                     /locus_tag="VNW28_07765"
     CDS             130..1146
                     /gene="hflK"
                     /locus_tag="VNW28_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HXA09865.1"
                     /translation="MQITTSSGSSASPFPPGFKFRPFRFVLGIVGVIGILLTLFSLLF
                     GFVSIPTNSVGVKLRFGAYHSTEGPGLAYAIPYVDEISIVPTQRLLKLEFGFTTPQAT
                     NGFQGDQQPEDTEMMITGDLNTALVPWVVQYRITDPKQYLFGAREPEQTLRDLSESVM
                     REVIGDRTVDEVLTIGRHDIETAALGRLAKLSSEYGLGLQIQQVQLATVRPPPAVQAA
                     FNEVNQAQQEKQTAINQAWAIYNDAVPNARGQAKEREKQAEAYAFRRVNGAKGDTEKF
                     LLLLTEYAKAPEITRRRLYFESMEAILPTLERKVIVDDSIKGVLQTLPLMAPAITPTP
                     TPQP"
     gene            1372..2406
                     /gene="hflC"
                     /locus_tag="VNW28_07770"
     CDS             1372..2406
                     /gene="hflC"
                     /locus_tag="VNW28_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HXA09866.1"
                     /translation="MDTIYRPQLPTRFVGFMQNPVLAIIGVVVLFVGLKWGTFLFTVH
                     QYETAIVTRFGKAIRVETTPGLKFKVPMIDAVHRFDNRILAWDGPPTETPTRDKLYLI
                     VDCFARWRISDPLLYYNRLNDERSALSRLDDILGSETRTAVASNDLVEIVRVTKGRQP
                     VRDEELEKSGTVLPSNLPDIELGRSAIEEQITRRARQKIEGFGIELLDERFKRSKYNP
                     AVAEKIIERMSSERHQIASKFRSEGRGEAANINGQKESDVASIQSAATRNALKIEGEA
                     DAEAARIYTAAYASPEAQNLYSFVRRLDVARHGFSQGTTAVLSTESDFGNVLKGIGAA
                     GIMPAPTPAR"
     gene            2447..2623
                     /locus_tag="VNW28_07775"
     CDS             2447..2623
                     /locus_tag="VNW28_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09867.1"
                     /translation="MTERNCSFLLCALATLLGCALSGCAADRQAQDDNPYHLEPAGSE
                     VHGEAGVSYGQSVH"
     gene            2693..3625
                     /locus_tag="VNW28_07780"
     CDS             2693..3625
                     /locus_tag="VNW28_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXA09868.1"
                     /translation="MIADPIKVERVTTDSKIFAYSRWDALPALAGLFHCAFFFALFFL
                     YPHTPLWLMLILGFIYALMINANINGVAHNFIHNPFFRAPLLNRLFGIVESVACCFSQ
                     TYYDVVHLQHHKGNSDRKNEAGETIDWLSIYRHGHDDEAENPWSYVFLSFFRDNPAAI
                     KRELRKRGASEVRWGNIELATVVLVMLTMFFLNWRYVVFYFLPFFYLGHCFSYLNGYF
                     RHYGADPDKPIAWGVSSYGKIYNWLFFYNGYHAEHHFRPKVHWTKIEEFHQQIADLQQ
                     QEGVRVIERAHMLGFLDPNLPPRKQGREPATLAS"
BASE COUNT          754 a         1147 c         1047 g          726 t
ORIGIN      
        1 aatctcgttg ctcgctggat cggcggcttt gagatagagg tggaatagca aaagagacca
       61 cattttctcg acgcctgttc cgcgcgcccg cttgcgtgcc gtgataggat cccttcgttc
      121 gttcccgtta tgcaaatcac cacctcttcc ggctcgtcgg cttcgccctt tccgcccggg
      181 ttcaagttcc ggccgttccg ctttgtcctc ggcatcgtcg gagtcatagg aatcctgctc
      241 acgctgttct cgcttctctt cggattcgtt tccattccga ccaactcggt cggcgtgaag
      301 ttgcgattcg gcgcctatca cagcacggag gggccgggtc tggcctacgc gattccttat
      361 gtcgatgaga tcagcatcgt gccgacccag cgcttgctga aactggagtt cgggttcacc
      421 acgccgcagg cgaccaacgg attccaaggc gatcagcaac cggaggacac cgagatgatg
      481 atcacggggg acctcaacac cgcgcttgtc ccgtgggtgg tgcaataccg gatcaccgat
      541 ccgaagcagt atcttttcgg cgcgcgcgaa ccggagcaga ctttgcgcga tctttcggaa
      601 agcgtgatgc gcgaggtgat cggcgaccgc acggtggacg aagtcctgac catcggccgg
      661 cacgatatcg aaacggcggc gctcgggcgg ttggcgaaat tgtcgtcgga atacgggctc
      721 ggtctgcaga tccagcaagt gcagttggcc acggtgcgtc cgccgccggc ggtgcaggcg
      781 gcgttcaatg aagtgaacca ggcgcagcag gagaaacaga ccgcgatcaa ccaggcctgg
      841 gcgatttaca acgacgccgt gcccaacgcc cggggccagg cgaaggaacg cgagaaacaa
      901 gcggaggcct acgcctttcg ccgggtcaat ggcgccaaag gcgacaccga aaaatttttg
      961 ctgctcctca cggagtacgc caaggcaccg gagatcacgc gacgccggct ttatttcgaa
     1021 tcgatggagg cgatcctgcc gacgctcgaa cgcaaggtga tcgtggatga cagcatcaaa
     1081 ggagtgctgc aaaccctgcc gctgatggcc cccgcaatca cgccgacccc gacgccgcag
     1141 ccctgaggag acggaggggt gagaaatgac gaattccgaa tgaccaaggc cgaaggaatt
     1201 ttaaattccg aagtccaaga ccggggcgtg cagaccgccg catcggtcat tggtccttcg
     1261 tcattccttc gtaaaccgtc attggccatt ggttattttc ccccgcctct tcgccgacag
     1321 aaaacttaac ctgtctgcat caaactaaag aattcaagga ctctaaccat catggacacc
     1381 atttatcgcc cgcaacttcc tacccgcttc gtcggtttta tgcagaatcc ggtgctcgcg
     1441 atcatcggcg tcgtcgtcct ctttgtgggg ctgaaatggg gcacgttcct tttcaccgtg
     1501 catcagtacg agaccgccat cgtaacccgt tttggcaagg ccatccgggt ggagaccacc
     1561 ccaggcttga aattcaaggt gccgatgatc gacgccgtgc atcgcttcga caatcgcatc
     1621 ctcgcctggg acggcccgcc cacggaaacg ccgaccaggg acaagcttta cctcatcgtt
     1681 gattgtttcg cgcgctggcg aatcagcgac ccgcttctct attacaatcg cttgaacgac
     1741 gagcgcagcg ctctctcgcg cctcgacgat atcctcggca gcgaaacccg caccgccgtt
     1801 gcctcaaacg atctggtcga gatcgtgagg gtaacgaaag gacgccagcc ggtgcgcgac
     1861 gaagagctgg aaaaaagcgg taccgtgctc ccgagtaatt tgcctgacat cgagcttggt
     1921 cgcagcgcga tcgaggaaca gatcacccgg cgcgcgcgcc agaaaatcga aggcttcggc
     1981 attgaactgc tcgatgagcg attcaagcgg agcaaataca atccggccgt agccgagaaa
     2041 atcatcgagc ggatgtcgag cgagcggcac caaatcgcct cgaagtttcg ctccgagggg
     2101 cgcggcgaag cagccaacat caacggacaa aaagagagcg atgtcgcctc catccagtcg
     2161 gccgcaaccc gcaacgcgct gaagatcgaa ggcgaagccg atgccgaggc ggcgcgcatc
     2221 tacaccgcgg cctacgcgtc gcccgaagcg caaaacctct actcgtttgt ccgccggctc
     2281 gacgtagcgc gccacgggtt tagccagggc acgacggccg tcctctcaac ggaaagcgat
     2341 ttcggcaacg tgctgaaagg gatcggcgcg gccgggatca tgcccgcgcc cacgccggcg
     2401 cgctaaattg cgcgggtcgc aagagcgcgt agaatgcgga cgcgcgatga ccgagcgaaa
     2461 ttgctccttt cttctctgcg ctctggcgac gctcctgggc tgcgccctca gtggttgcgc
     2521 ggctgatcgc caggcgcagg acgacaatcc ctatcacctc gagcccgctg gcagcgaggt
     2581 gcacggcgaa gccggcgtct cttacggcca gagcgtgcat tagcgcccct tgatttatcc
     2641 gccctgtttc acgagacttt ttgcccccag accattgccg gcggctcttt ccatgatcgc
     2701 tgatcccatc aaagtagaac gcgtcacgac cgattcgaaa atcttcgcct acagccgctg
     2761 ggatgcgctg ccggctctgg cgggcctttt tcattgcgcc ttcttcttcg cgctcttctt
     2821 tctctacccg catacgccgc tctggctgat gcttattctt gggttcatct acgccttgat
     2881 gatcaacgcg aacatcaacg gcgtggcgca taattttatc cacaacccgt tctttcgcgc
     2941 gccgctcctc aaccgccttt tcgggatcgt cgaatcggtc gcctgctgct tctcgcagac
     3001 ctattacgac gtcgtccatc tccagcacca caagggaaac agcgaccgga aaaacgaggc
     3061 gggcgagacg atcgactggc tctcgatcta ccgccacggg cacgatgacg aggcggaaaa
     3121 tccgtggagc tatgtcttcc tcagcttctt tcgcgacaat cccgcagcga tcaaacgcga
     3181 gctgcgcaag cggggcgcga gcgaggtgcg ctggggcaac atcgaactgg cgacggttgt
     3241 gctcgtcatg ctgacgatgt tcttccttaa ctggcgttac gtcgtttttt atttcctgcc
     3301 cttcttttac ctcgggcact gcttcagcta cctgaacggc tatttccggc attacggcgc
     3361 cgaccccgac aagccgatcg cctggggcgt gagcagctac ggtaaaattt ataactggct
     3421 ctttttctac aacggctacc acgcggagca tcatttccgg cccaaggtgc actggacgaa
     3481 aatcgaagag ttccaccagc agatcgccga cctgcaacag caggaaggcg tgcgcgtgat
     3541 tgagcgcgcc cacatgctcg ggttcctcga tccgaatctc ccgccgcgaa aacagggccg
     3601 cgagccggca acactggcgt cgtaattgta gccgccgccc tgtgggcggc gcggcgcagg
     3661 acgtcgctac tgga
//
LOCUS       DATMNS010000222         2239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4463105, whole genome shotgun sequence.
ACCESSION   DATMNS010000222 DATMNS010000000
VERSION     DATMNS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4463105"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VNW28_07785"
     CDS             complement(<1..260)
                     /locus_tag="VNW28_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HXA09869.1"
                     /translation="MKKASLPKARPKNGLTSKSDEVNAIELLAALMAFKRGDFTARLP
                     NDWTGVAGKIADTFNDVIATNQRMTRELERIGTVVGKQGRIT"
     gene            454..723
                     /gene="rpsN"
                     /locus_tag="VNW28_07790"
     CDS             454..723
                     /gene="rpsN"
                     /locus_tag="VNW28_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HXA09870.1"
                     /translation="MAKTSWINRNERKRKTVAKYAALRAELKAKKDYIGLSQLPRDAS
                     PTRVVNRCQVTGRRRAFIRRFKISRLTFRELASAGLIPGVTKSSW"
     gene            772..2157
                     /locus_tag="VNW28_07795"
     CDS             772..2157
                     /locus_tag="VNW28_07795"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HXA09871.1"
                     /translation="MTSIFHFFAFALVAHGVSTQWDSPAILLGKLATIAALVALNGFF
                     VAAEYSIIKVRSSQLDTLIDEGERRAIFARHVRAHLDSYLSATQLGVTLASLGLGWAG
                     EQFLASILQPFFGLIHVDSLAIVTTISVVLAFLGITFLHIVFGELAPKYVAITNPLPV
                     SLRLVRTLAMFHLIFRPFIWLLNKSSNFFLQKMLRLKPVSGAELAHSEEELRLILDQS
                     EKSDEVSSIGRDILVNALDMRRRVVRDIMTPRGEVVYLDIEESFDENVKKALESRHTR
                     FPLCRGHLDNTIGLIHIKELVPLMRDPHPDLAKIKRELIPVPEMMSLEKLLNFFLVRH
                     AHLAVVVDEYGGTVGIVTMENVLEELVGDIQDEFDTEKAEFRKINDHEFMVNGGLGLY
                     ELRDLANIEVENSDVSTVGGYVTHLLGHLPKQGEQVPIEDYIVTVSQTDGRRVGQLHF
                     KKMADDDGQTE"
BASE COUNT          430 a          671 c          657 g          481 t
ORIGIN      
        1 tgagtgatcc ggccttgttt tccgaccacg gtgccaatcc tttccagctc ccgcgtcatt
       61 cgctggttgg tcgcaatgac atcgttgaag gtgtcggcga tcttgcccgc cacccctgtc
      121 cagtcgttgg gcaaccgggc cgtaaaatcg ccgcgcttga acgccatcaa cgctgcgagc
      181 agctctatgg cattgacttc atcgctcttg gacgtcaaac cgtttttcgg ccgcgcttta
      241 ggtagagagg ctttcttcat ggatggaagt gcacaagcct aaatcgcgac aaaacagcga
      301 tccgaccggg tccgatgcag atccagccgc tgattttaag ccctggatca gagcggatta
      361 agcctttcgc ggaaaacaac cggtccatgc gggctggccg aacgagaaaa tcggttgcgc
      421 caggcggttt tcccgctaac ttccgcgttc cttatggcga agacttcgtg gatcaatcgc
      481 aacgagcgca agcgcaagac ggtggcgaaa tacgccgccc tgcgcgctga gctcaaggcg
      541 aaaaaggatt acatcggcct ctcccagctc ccgcgcgacg ccagccccac ccgcgtggtc
      601 aatcggtgcc aggtcaccgg ccgccgccgc gcgttcattc gccgcttcaa gatttcccgc
      661 ctgacctttc gcgagctcgc ttccgccggt ctcattcccg gtgtgacgaa gtcgagctgg
      721 tagcttggcc cgcgctgggc cccgcggggc gcgacgattt gcgcccgccc gatgacctcc
      781 atttttcatt tttttgcttt cgccttggtc gcccatggcg tctccacaca atgggattca
      841 cctgccatct tactgggtaa attggcgacc atcgccgcgc tcgtggccct gaacggtttt
      901 ttcgttgccg cggagtattc catcatcaaa gtgcggagca gccagctcga cacgttgatc
      961 gacgagggcg agcggcgagc catttttgcc cgtcacgtcc gcgcgcatct cgattcctat
     1021 ctttccgcga cgcagctggg cgtgacgctg gccagccttg gattggggtg ggccggcgag
     1081 caattcctcg cctcgatcct gcaaccgttc ttcggactga tccatgttga ttccctggcg
     1141 attgtgacca cgatttccgt ggtgctcgcc tttctcggga tcaccttttt gcacatcgtc
     1201 tttggggagc tggcgccgaa gtacgtcgcg atcacaaatc cgctgccggt ttcgctccgg
     1261 ctggtgcgga cgctggcgat gtttcatctg atctttcggc cgttcatctg gctgctgaat
     1321 aaatcgtcca attttttcct gcaaaagatg ctgcgcctca agccggtcag cggtgccgag
     1381 ctggcgcata gtgaggagga gctgcgcctg attctcgacc agagcgagaa atccgacgaa
     1441 gtctcctcca tcggtcgcga cattctggtc aacgcgctcg atatgcggcg ccgggtcgtg
     1501 cgcgacatca tgacgccgcg tggcgaagtg gtctatctcg atatcgaaga aagcttcgac
     1561 gagaacgtga aaaaggcgct ggaatcaagg catacccgct tcccgctgtg ccgcgggcat
     1621 ctcgacaaca cgatcggcct gatccacatc aaggaactgg tgcctttgat gcgggacccg
     1681 cacccggacc tggcgaagat caagcgcgag ctgatcccgg tgccggaaat gatgtcgctc
     1741 gagaagttgc tcaacttctt cctcgttagg cacgcgcatc tcgcggtggt ggtggatgaa
     1801 tatggcggca ccgtcgggat cgtgacgatg gaaaacgtgc tcgaggaatt ggtcggcgat
     1861 atccaggacg agttcgacac cgaaaaggcg gagttccgca aaatcaacga ccacgagttc
     1921 atggtcaacg gcgggctcgg cctctacgag ttgcgggatc tcgcgaatat cgaggtggaa
     1981 aattccgatg ttagcacggt cggcggttat gtcacgcact tgctcggtca tctcccgaag
     2041 cagggcgagc aggtgccgat cgaggattac atcgtcaccg tttcacagac cgatggacgc
     2101 cgcgtcggcc agctgcattt caaaaaaatg gcggacgacg acggccagac ggagtagcgg
     2161 gcgtcccgga gcaacgcgtg cccgactggt ggagcgcgct aaaactggtc gaaatccgcg
     2221 acgacgtgga tcgtcccag
//
LOCUS       DATMNS010000223         2154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5333329, whole genome shotgun sequence.
ACCESSION   DATMNS010000223 DATMNS010000000
VERSION     DATMNS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5333329"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..323)
                     /locus_tag="VNW28_07800"
     tRNA            complement(251..323)
                     /locus_tag="VNW28_07800"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Glu,seq:ttc)
     gene            complement(372..1664)
                     /locus_tag="VNW28_07805"
     CDS             complement(372..1664)
                     /locus_tag="VNW28_07805"
                     /inference="COORDINATES: protein motif:HMM:NF016311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HXA09872.1"
                     /translation="MIRFFYNLLFPLALLFFLPGYAVKMLRRGNYRNKFGQRLGFYDR
                     ATRAALAAGPQTWLHAVSVGEVMIALKLASKMKEHEPALRIALTTTTTTGFALANKQA
                     PGWIEVLYTPLDFWPIMSRAFEIIRPTRVLLVEAEVWPNLTAIAHWQKIPLALVNARL
                     SPRSEQRFRKFNYFVRPYFQKLDLICVSEPDDVARWVSLGVDSVRIHPVGSIKFDPEN
                     DTPRPGTPRAVLDNFGIDPTRPILLAGSTHPGEEEIFARIFLALRREFPDLFLVIAPR
                     HVERTRAIAAQLKVFGLTTVRRTEPDPATASDCLLLDTTGELRDWFAVATIVFIGKSL
                     TARGGQNPVEAIVADRPVVFGPHMENFASLARALVAAGGALQSDDEVALTKILGNLLR
                     DAPARERLVRLAREVLDIHRGATERTAQLLENLAARAV"
     gene            complement(1849..>2154)
                     /locus_tag="VNW28_07810"
     CDS             complement(1849..>2154)
                     /locus_tag="VNW28_07810"
                     /inference="COORDINATES: protein motif:HMM:NF015559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HXA09873.1"
                     /translation="QTGLSGRVRKQDVFDFLSHASTAEPHDLIFADPPYDQSKLGDAF
                     TRLLLRNDRLPARLTNEGIFVLEKRPAERLPTTDFWEIVRRKTYGATETIFLRKKSP"
BASE COUNT          421 a          589 c          712 g          432 t
ORIGIN      
        1 ccgactacga attgtcttgg gttgagacgg tgacgcttgc cgccgaggtg ataggcgacg
       61 cgaaaataag tgccttgcgg tttttgatcg cggtaaattt tgaccgcgca ggagccgcgt
      121 ttaacgacaa tcgggaattt cggtttcttc gtgttcatag cacgaatttg cgctaattct
      181 gtccgaaaag tgtccgaaat tgcaagcaaa atctttcgct cgctggcatt ttacctatga
      241 gaataaatag cgaccctaac gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc
      301 ctaggcctct ggacgatagg gtcaggaaga gcgcccaata tccggagcag tcacccgcca
      361 tgcaaggtat ttcaaacggc gcgtgccgcg agattttcga gtaactgcgc ggtccgctcg
      421 gtcgcgcccc gatggatatc gagcacttcg cgagcaaggc ggacgagccg ttcccgggcc
      481 ggcgcgtcgc gcaggagatt gcctaaaatc ttcgttaggg cgacttcgtc gtcggactgc
      541 agcgcgccgc ccgccgccac cagagcgcgg gcgagggacg cgaagttttc catgtgcggg
      601 ccaaagacca ccgggcgatc ggcgacgatt gcttcgaccg ggttttggcc gccacgcgcg
      661 gtcaggcttt tgccaatgaa gacgatggtc gcgacggcga accaatcacg cagctcgccg
      721 gtcgtatcga ggaggaggca atcgctcgcc gtcgccgggt cgggctcggt ccgtctcacc
      781 gtcgttaggc cgaagacttt tagctgggcg gcgatcgcgc gcgtccgctc gacatgccgt
      841 ggggcgatga cgaggaagag atcgggaaat tcgcgccgca gcgcgagaaa aatccgggca
      901 aagatttctt cttcgccggg atgggtgctg ccggcgagca ggatcgggcg tgtcggatcg
      961 atcccgaagt tgtccagaac ggcgcgcggc gtcccgggcc gcggcgtgtc gttttctgga
     1021 tcgaatttaa tgctgccgac gggatgaatc cgcaccgagt ccacgcctaa cgacacccag
     1081 cgcgcgacgt catccggttc tgacacgcag atcagatcga gtttttgaaa gtagggccta
     1141 acgaaataat taaactttcg gaagcgttgc tccgaccgcg gcgagagccg tgcattgacg
     1201 agtgcgagcg gaatcttttg ccagtgcgcg atggcggtca ggttgggcca gacttccgct
     1261 tcgacgagca agacgcgcgt tgggcggatg atctcgaagg cgcggctcat gattggccaa
     1321 aagtcgagtg gagtgtagag cacttcgatc cacccgggcg cttgcttgtt ggccagcgcg
     1381 aacccggttg tcgttgtggt ggtaagggcg atgcgcagcg ccggttcgtg ttccttcatc
     1441 ttgctcgcga gcttcagcgc gatcatcacc tcgccgacgc tgaccgcgtg caaccaggtc
     1501 tgggggccgg cggccagggc ggctcgcgtg gcccgatcgt agaatccgag ccgctgaccg
     1561 aacttgtttc ggtagttgcc gcgccggagc atcttcaccg cgtaacccgg gagaaaaaaa
     1621 aggagggcca gcgggaaaag caggttgtag aagaagcgga tcatcagtgc ccgggtagcg
     1681 cacgcgtctc gcgtgctggc gacggtgttc caccgtcgcg aacttttcga gagattggag
     1741 tcgttggggc aacgcgaacg gaaagaaaaa gttcgccgcg gtggaacacc gcgaccggca
     1801 cgcgagtcgc gtgcgctacc cggaggttcg gcgacccgct ggatgacttc atggtgattt
     1861 tttccgcagg aagatcgtct cggtcgcgcc gtaggttttg cgccgcacga tttcccaaaa
     1921 gtccgttgtc ggcaggcgtt cggctggtcg tttttcgaga acgaaaattc cttcattcgt
     1981 taggcgagca ggcagacggt cgttgcgcag aagcaacctg gtgaaggcgt cgccaagttt
     2041 cgattggtcg tagggcgggt cggcgaagat cagatcgtgc ggctcggcgg tactggcgtg
     2101 gctgagaaaa tcaaagacgt cctgtttccg aacgcggccg ctcaggccgg tttg
//
LOCUS       DATMNS010000224        21201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3027646, whole genome shotgun sequence.
ACCESSION   DATMNS010000224 DATMNS010000000
VERSION     DATMNS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 21201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21201
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3027646"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /locus_tag="VNW28_07815"
     CDS             complement(<1..652)
                     /locus_tag="VNW28_07815"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HXA09874.1"
                     /translation="MLSIRMRAALICLAFLLASGLQGAPLTRAEVTRIINVVDVVVPR
                     KGAHPAVLHEVIKDEIGLRTGSKSRSELMFEDHTLTRIGPETYFSFKAGTRDITLQQG
                     TILLQVPKGLGGVRLRTAGVTAAIAGTTMMMEYVPAKHLKVLVLEGSLRLSRNGAYGD
                     SLLLTPGKMVIMRPSAKRIPDPVSVDLAHVIKTSSLVNLTDKKDAQPLPGMALIKKE"
     gene            860..1798
                     /locus_tag="VNW28_07820"
     CDS             860..1798
                     /locus_tag="VNW28_07820"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXA09875.1"
                     /translation="MMTRDSSELAAASLSVRFWGVRGSIPSPGPETAGYGGNTSCVEL
                     RVGNEVLVLDAGSGIRRLGIALAHELVGNPLTTTLLISHTHWDHIQGLPFFRPAYSRA
                     SQIRILGAPGTRTKLRAALTSQMDPIQFPVSIECLAGISAIDEFAAGTTTIGSFVVRT
                     IALNHPGGCTGFRIDAGGTSVAYLPDHEPYRSSTIAFDSVVAAATGQAETEFLDFLEG
                     CNLLILDSQYDRSEYPGHVGWGHGCLEDSVALALRAGVRSLVLFHHDPDHDDQKIDSM
                     VQQARQQVADAGSRLQVHAAREQEQLVPRARHQLAA"
     gene            1857..3818
                     /locus_tag="VNW28_07825"
     CDS             1857..3818
                     /locus_tag="VNW28_07825"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXA09876.1"
                     /translation="MSLKRILLLSGIATLCFAALFLEDRWQPLVLFRLRNMARDAVAR
                     AGRTTPPNPALVFLAIDSDSVGLDATTDVKQLYGLSDKQSVEAHALELMSQAWPWSRE
                     VYALVLRRLVDAGAKVVAFDLTFPTATPEDPPFRLALDRYKEHVVIGSNFISSASRGF
                     ATVDASLTRPPESLVPETQPMDDRVAFTNFWPEEDEVVRQAQFRITFEQVQGNLPGPK
                     TERFLSFAAQTLIKAGRADAVPGGLDERLFRFTAPPRIGFRPHSLFEIFVPDYWQHNY
                     QSGEFFRGKIVVIGAEGNWQHDEHPTPFGSMPGPELHLNAINAALHHEFISEMSPLAV
                     VSFTIAAGLLAIFLSLTIRSPWLRLLALIVGDAGWAWFAIFVFNHASVYLPIATPYLQ
                     LNLTLLLGLAADFTLERVEKKRVRQALERYVSRDVVREMLDKPKLYEQSLGGVLKRVT
                     ILFSDIRGFSKVSAQSDPQVLVRQLNEYLTAMVECVFRFGGTLDKFMGDAVMAVWGNV
                     RTAGAGDDAVAAVEAALAMRAELARLNQKWRAEGLPELKIGVALNHGAVVAGNIGSPQ
                     RMEFTVIGDAVNVTWKMQELTKRVGAELIVSKTVEALIVEHFELHPLGQFALHNLPGA
                     WEVFAISEPIAAPRERAPRPLPQNAAKET"
     gene            complement(3805..6906)
                     /locus_tag="VNW28_07830"
     CDS             complement(3805..6906)
                     /locus_tag="VNW28_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09877.1"
                     /translation="MKSRFRILFFLLPILALGEYASAEQDFSNLLRWRNIGPYRGGRT
                     RAVSGVPSQPNVFYCAQVDGGVFKTNDYGRTWRPIFDDQPTGSVGAIAVSVSNPEVIY
                     VGSGEGLHRPDLSVGDGIYKSTDTGKTWAHLGLRDGQQIAQIAVDPHNPDHVLVAVAG
                     HPYGPNEERGIFLSRDGGKTFAKTLYQDENTGAADVQIDPQNPETVYAALWEAREGPW
                     ENAEWNGTSGGIFKSTDGGKTWKRLTKGLPNDIVQANIAIAANAPQTLVAAVATLHAG
                     KLYRSDDAGESWTLATTDERPGARIGGGDLPLVRIDPQNPKVVYAASVVCWKSTDGGK
                     TWDGWRGAPGGDDYQNVWINPANSNIVLLGSDQGAIITVNGGASWSSWYNQSTAQLYH
                     VSADNAFPYRLYSGQQESGSVGITSRGPDGAITFRDWHPVAAEEYGYVVADPLDPDII
                     YGGKLTRYDRRTHQAQSILPQPFRTSDFRMIRTQPILFSPLDPHLLLFATNYLWQTRD
                     GGQHWTQLSPDLTRATFEVPASVGKFRSQPTAQPRQRGVIYAVGPSPLDKNRIWAGTD
                     DGLVHLTTDDGKTWNDVTPSQIGPWWKVSVMEASHFDQNTAYAAVNTIRLDDLRPHIY
                     RTSDAGKTWKEIVQGISPNENVNTVREDPQRRGLLFAGTERAVYVSFDDGENWQSLRL
                     NMPATSIRDLIVKEDDLAIATHGRGFWIMDNITPLRQRQPNEKATCLFQPEIALRVRW
                     NFNTDTPLPPDEPVGENPPDGAMIDYSLGKDVAGPVTLEIKDGQGKVLRRYASTDPLP
                     TPDPKLKIPRYWVRPPQALSAEPGLHRFFWDLHGEPLAGVEPQYPMRAVAHETAPQPT
                     APWVVPGNYSVALTTGGKSFTQPLIVKMDPRVKASAADLAKQFELSEALTETRVTLQP
                     IGRSFKALAAELGKAKERLGKNAARGAVAALRQKLEGFTNPADVRPGEPLELDVLRKV
                     ETLFGDLQGVDGVPTPAQQAAVAALQRDARSVVERWRALPPEVAALNAQLQAGGLEPI
                     KFP"
     gene            complement(7097..7660)
                     /locus_tag="VNW28_07835"
     CDS             complement(7097..7660)
                     /locus_tag="VNW28_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXA09878.1"
                     /translation="MMIGAGILKGMAVTGRNFLGSYFEKDRLTTVQYPEERDVLPENS
                     RNFPFLIYDTNDPEAGLRCVACKICEKECPPQCIYIEKSADKKPDYMGKPQFYPATFD
                     IDISVCMSCQICVEVCPFEAIKMDKVYELSRRERFDALLMRKSDLAKSNSYYHSIHPL
                     EAEQVDDALAAAAAKKKPAPTPAPSPG"
     gene            complement(7657..8817)
                     /locus_tag="VNW28_07840"
     CDS             complement(7657..8817)
                     /locus_tag="VNW28_07840"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-quinone oxidoreductase"
                     /protein_id="HXA09879.1"
                     /translation="MSGTGLHELDQAPPRRTSRLDGELLEVSLGPQHPSTHGVFRMNV
                     ALEGEMVRKLKPVFGYLHRNHEKIGENTSYLGSMPYTDRLDYICSMSNNWGYALSVER
                     LAGIEVPERAEYLRIILAELTRLVNHASLLGFLLSDMGAWGTPLMYAFRERENILDLF
                     ESLSGSRMMCDYMRFGGCRCDLPTGWLGRARKIVDGFPRFLDEFERLITGNEILIART
                     QNVGKLSPELAINAGITGPMLRASGVNYDIRKVDGYGFYPRFKFRVPLGEHGDTYDRL
                     MMRVLELRETLGLLRQAMDDLPAGPIMNPKAKLRAFKPPLGEAYGRIEGPKGELGFYL
                     ISDGSGNPYRYRVRPPSFINLTILEDLCLGHTVADVMVILGSVDIVMGEVDR"
     gene            complement(8814..9482)
                     /locus_tag="VNW28_07845"
     CDS             complement(8814..9482)
                     /locus_tag="VNW28_07845"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXA09880.1"
                     /translation="MMEALDEIKFRIEAAIPGARLEIIPNPGPANQSSLVLDHAHAST
                     VARFLRDDPTLRLDYCSNVTGVDWLERTAKKKVRVKKLVEGEEKEVEETQEEFFPGYL
                     EAVYHLYSMAHKHGPVIVRLRTRDRAEGTPLPSLTPVWRSAEFQEREIYDLYGIRFDG
                     HPDLRRILMWDEFTDHPMRKDYREPDDYEYEPTPHDDVLEKAKQHYPSRPAQDGTENI
                     AAQT"
     gene            complement(9582..10211)
                     /locus_tag="VNW28_07850"
     CDS             complement(9582..10211)
                     /locus_tag="VNW28_07850"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF011394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HXA09881.1"
                     /translation="MASAPPIDQGLRSELQKQGIWVTSMQELYNWGRRNSIWPLQFGL
                     ACCAIEMIATAAARYDLARFGGEVFRPSPRQADLMIVAGTVTKKMAPQIVRLYNQMPD
                     PKYVISMGACAISGGPFKQGYNVLKGIDRYIPVDVYIPGCPPRPEALLHAFMELQRKI
                     DGQKLTGTGKADFLDRTVPSEFPVPAFGAHDLEPPANPDTFHPPKIERH"
     gene            complement(10211..10585)
                     /locus_tag="VNW28_07855"
     CDS             complement(10211..10585)
                     /locus_tag="VNW28_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXA09882.1"
                     /translation="MTPSPYFFLVIFGAVAFVFPLLPLALAWLWRHFFQPPKPGARKN
                     ATYECGVESLGEAQIQFHSQYYLYAIIFLIFDVEAIFLVPFAVAFTRLPFGAFLAILV
                     FLLLLLEGLVWAWGKGCLSWVK"
     gene            complement(10728..12089)
                     /gene="gdhA"
                     /locus_tag="VNW28_07860"
     CDS             complement(10728..12089)
                     /gene="gdhA"
                     /locus_tag="VNW28_07860"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HXA09883.1"
                     /translation="MKDYVTELMAGVKAKNPAEPEFHQAVQEVVESLALVLERHPEYR
                     KAKILERMIEPERLIMFRVPWEDDKGEIHINRGFRIEMNSAIGPYKGGLRFHRSVNLG
                     ILKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSFMAELFRHIGAYT
                     DVPAGDIGVGGREIGFLFGMYKKLRNEFTGVLTGKGLSWGGSLIRPQATGYGAVYFAA
                     EMLKTRKEGLEGMTCLISGSGNVAQYTIEKLINMGAKPVTVSDSSGYIYDEAGINDQK
                     LSFIMDLKNVRRGRIAEYAEKFKGAVHIPFDPNLDHNPLWNHKAQCAFPGATQNEING
                     KDAANLLRNGVYVISEGANMPTTIEGVNQFLDAKILYGPGKAANAGGVATSGLEMAQN
                     SMRISWTRDEVDSRLQTIMKAIHRACYQTSETYGTPGNYVNGANIAGFLKVANAMMDQ
                     GVV"
     gene            12363..13589
                     /gene="nuoH"
                     /locus_tag="VNW28_07865"
     CDS             12363..13589
                     /gene="nuoH"
                     /locus_tag="VNW28_07865"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXA09884.1"
                     /translation="MLKKADLAFFHRNVSGFLDQIFILGKRWVLSFLAGAPDWVGQIA
                     SSLLNIIALLGVFLSLFALMSLFERKILARIQNRYGPNRVGPFGLLQPVADGIKMLIK
                     EDIVPARADKVVHFLAPILIAAAAILALGIIPYGRNMTPFTIDGGILFFFAVGSTNEV
                     GVFMAGWGSNNKFSMLGAMRAIAQMVSYELPLIITVLPVVMVTGSLTPDAIVAAQGGY
                     SLGVLPHWFVFTPWGAAAFILFFVSGLVESNRTPFDVPEGESEIVAGHMTEYSGFKYA
                     IFFLAEYFGMFAVSGLAVTLFLGGWHAPLRVLEFVPSYLWFFLKLGVLLFIFIWIRGT
                     LPRVRVDQMMSFAWKFMLPMAFTCILAAAVWHYAGHRLAGWFLSLVVVLLVYLALSRL
                     LGAGKKFAPRTYRFAE"
     gene            13586..14068
                     /locus_tag="VNW28_07870"
     CDS             13586..14068
                     /locus_tag="VNW28_07870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXA09885.1"
                     /translation="MNSAAFFLIAVLTLSAALAAASFRQLVHAALSFAVALVGVAAFY
                     FLLGAEFVGLVQIFVYVGAVAVLIVFTILLTHREPEEHRGFNWSGAVIALAVFAGLLW
                     AIVRTPALYVAAQPGTAITVRQIGEALMSDYVWPLQCVGLLLTAALIGALVLVLEEKR
                     "
     gene            14211..14525
                     /gene="nuoK"
                     /locus_tag="VNW28_07875"
     CDS             14211..14525
                     /gene="nuoK"
                     /locus_tag="VNW28_07875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXA09886.1"
                     /translation="MTPTLATFLIISAALFCLGLLAALSRRHAIFILIGIEMMLAAAG
                     LNFIAFWRFGPQPAPPTGVMVTIFAIAIAAAEAAVGLALVIAVYRHYRTTSVDRIDQL
                     KG"
     gene            14522..16495
                     /gene="nuoL"
                     /locus_tag="VNW28_07880"
     CDS             14522..16495
                     /gene="nuoL"
                     /locus_tag="VNW28_07880"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXA09887.1"
                     /translation="MSNWIVVHLWLIPAVPLAASLVILGLAKSRRAAAAGLAIAGQVV
                     ALLLSLDAFWPTLQTPGDRVFQNFTWFTFGEQTLRLGFVLDPLAAVMLVMITLVSLCI
                     FVFSVGYMAADKNFTRFFAYLSFFSAAMLGVVIANSLLLLFVCWELVGLASYLLIGFW
                     IHKPSAAAAAKKAFITTRIGDIGFFLGILWLSWRSGTLLFYDGGNGALESAGLLSLGA
                     SATFIALLIFCGAAGKSGQFPLHVWLPDAMEGPTPVSALIHAATMVAAGVFLVARVYP
                     LFSLGAINGVTPSLTVVVWIGVITALISALIALAQPDIKRILAYSTVSQLGLMMVSLG
                     VGGVAAGIMHLLAHGFFKALLFLGSGSVIHGCHEEQDIRRMGGLRGLMPVTFFTYAIG
                     MLALSGVPLFFSGAWTKEEILHATSQWAPSRVPHYLMLAGVILTALYMTRQMIYVFFG
                     ERRAAAARAHESPPVMTLPLIVLAICAVGFSVVLTPAWPWLHDYLSGERATYNLSLLV
                     QPMLFVSLALVAAGVGLGIGFYRSASEIDPLERALPALFRFLENRMWLDELYDWTILS
                     WARFAARLSDFLDRYLWDGLVRLVGGLGRVGGGLTKGLDERGINAGVDDATRVTRGLG
                     RFVSAGHSGQVQVYLSVIAVGMLALLLLYAWLT"
     gene            16483..17955
                     /locus_tag="VNW28_07885"
     CDS             16483..17955
                     /locus_tag="VNW28_07885"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXA09888.1"
                     /translation="MAHLISGLIFIPLLGALFLSVVPSTLARPAALGCNFITGCLALS
                     IWYHFDAANPALQMVQRHVWIPAIGAQALAGIDGLSLLLVLLTSLIFPFAFCAGRMSR
                     GFYSIMLVAQAALYGTFTAQNFILWFLFYEMSLIPAFLLIRIWGGEKRDRAATKFFLY
                     TFLGSVALLLSFLYIYFVRGTFDFAELASLGKNGLLEGNIRWLAFAGIFLGLAVKVPI
                     FPFHTWLPDAYEAAPTGASMVLTGLLSKMGVYGFIRLLLPLFPNEIRIAGPWLLGLAV
                     CSIVFASLAAWAQSDLKRMVAYLSINHLGYCMLGLFAVTATTSAPPVEAQAALSGVFL
                     QIFNHGLTAAALFYFVGLLEERRGARGLGDFGGLMQRTPLLCAWMSVAMFSSLGLPGL
                     NGFVGEFLIFKGSFALAAGYTAVAVLGLLFTAIVFLRAMQALFSGTLAESCSQFPDLR
                     LGEKLVVVPVTLLMFAVGLYPQFVLHIFNSTVAQMARLSG"
     gene            18257..19714
                     /locus_tag="VNW28_07890"
     CDS             18257..19714
                     /locus_tag="VNW28_07890"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXA09889.1"
                     /translation="MIAWAIYLTFAGALLALLLPRILARWIALVTTAGGFALGLAALF
                     ATNADFAHFTTIVRVPWVPALGMNYHLAADGISLTMTLVTGLSATAAVLFSWKVENRP
                     NEFFFWLLLVVAGSYGVFLSADLFLFFVFYELVIVPKYFLIAIWGSTNKEYGAMKLTL
                     YSFLGGALVFIGILVAYVTAGTLDLPQLAHFQFAPQLQAWAFPVLFLGFAVLGGIWPL
                     HTWAPTGHVAAPTAGSMLLAGIVMKLGAYGALRVALNLFPLGFQMWSKWIAVFAVIGI
                     VYAAAVALRQSDLKFVIGYSSVSHMGFVLLGLATANVLGVSGAVLQMFSHGVIGALLF
                     ALAGAVYYRTHTRNLNDLSGMGLSRAMPFLTFAFVVASAASMGIPGFSGFAAEITILI
                     GAWKAFPLAVGITGLGMVLVAAFTLRALKKSFFGENPTGRVWPETSAPSAAATLAEKL
                     GASLLIGATLAVGLCPNLLLDRIMPAVETMRFLHR"
     gene            19711..>21201
                     /locus_tag="VNW28_07895"
     CDS             19711..>21201
                     /locus_tag="VNW28_07895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HXA09890.1"
                     /translation="MSYLELLQLAAPEAIVVVTALVVLTIGLVGGRQEAGDTLAATPV
                     LGSLVAAFGLAIAAAAIVRLPGHAMLFHGMLVISPLNSLFQVIVLVLAFSTVVLTRGE
                     HSLPNKSEYLAMILLATVGLMLLIGSEELLMIFIGLELTGLSLYVMTAFDKASQPSAE
                     AGLKYFLFGSMASAFTLFGLSFIYGFTGTTSLAAIGPRLAEQALPPLLFVGIVMTLIG
                     FFFKIAAAPFHLWAPDAYQGAPISTAGFIASASKVASFVVLGKILLIGFGSVPGSAAW
                     HGMVAGWMPILAVLSALSILIGNLVALVQKNVRRLLAYSAVAHGGYTLLGLIAGGRAG
                     FSATLFYTTIYAVTLVGAFGVVGFVRRENGGDDLADFAGLRVRSPLLAGCMAVFLLSL
                     AGLPPLAGFFGKFYLFSVALRAGENEGLLWLVVLALFGSLISLYYYLIVLKVIFLDAN
                     AKPAPAVARYDFEVRADYLSKVVLLVLAGTVLLLGIIPGTLVARILA"
BASE COUNT         3432 a         6701 c         6384 g         4684 t
ORIGIN      
        1 tttccttctt gatcaacgcc atgcccggca gcggctgagc atcttttttg tcggtcaggt
       61 tgactaacga ggaggtctta atcacgtgcg cgaggtcgac cgagacggga tcgggaatgc
      121 gctttgcgct ggggcgcatg attaccatct tgccgggtgt gagcagaagc gagtcgccgt
      181 acgcgccgtt gcgcgaaagg cgcaggctgc cttccaaaac gagcacctta aggtgtttcg
      241 cgggcacata ttccatcatc atcgtggtgc cggcgatcgc cgcagtgacg ccggccgtcc
      301 gaaggcgcac cccgccgagt cccttcggaa cctgcaataa tatcgtgccc tgctgcaggg
      361 taatgtcgcg ggtgcccgcc ttgaagctaa agtaagtttc cggtccgatg cgggtcaggg
      421 tatggtcttc aaacatcagc tccgagcgtg atttgctgcc cgtacggagt ccgatctcat
      481 ccttgatgac ttcgtggagg accgcggggt gagcgccttt ccgcggcaca acaacgtcga
      541 cgacgttgat gatcctggtt acttccgccc gggtgagcgg ggcgccttgc aagccggaag
      601 ccagaagaaa agcgaggcag atgagggcag cgcgcatgcg aatggaaagc atccaatagc
      661 cattgagcag caagcatacc ctaccgcgcc atttattagg gcacgccggg ttgaatttta
      721 gcagggacta ccctgtcgcc gaaccgctaa ggcggcggcc ggcgaagagg gaggcatggc
      781 tgatgctatg aactgccgga gaactcctcg ccaacactta tgcgcctaac gaccggcaac
      841 attccgcctg cgcgctccca tgatgacgcg tgattcatcc gaactcgctg cggcgtcgct
      901 ctccgtccgt ttctggggag tgcgcggttc gatcccttcc ccgggaccgg agacggcggg
      961 ctacggcggc aacacttcct gcgtcgagct gcgagttggg aatgaagtcc tcgtcctgga
     1021 tgccgggagt ggcattcggc ggttggggat cgccctcgcg cacgagctcg tgggaaatcc
     1081 gctcacgacc actttgctca tttcgcacac ccactgggat catattcagg gactgccctt
     1141 cttccggcca gcctacagtc gcgccagcca gatccggatt ctcggcgccc cgggaacccg
     1201 gacgaaactg cgcgctgcgc tcaccagtca gatggatcca atccagtttc cggtttccat
     1261 cgagtgcctc gccggcattt cggcgatcga cgaatttgcc gctgggacga ccaccatcgg
     1321 cagctttgtc gtgcgcacca tcgcgctcaa ccacccggga gggtgcaccg gttttcggat
     1381 cgatgccggc ggcacgtccg tcgcctatct gcccgatcac gagccctacc gcagctcgac
     1441 catcgctttt gattcggtcg tggcggcagc gaccggccaa gccgaaacgg agttcctcga
     1501 ttttctcgaa gggtgcaacc tcctgatcct cgattcgcaa tacgaccgga gcgagtatcc
     1561 cggacacgtc ggctggggcc acggctgcct cgaggacagc gttgcgctcg cgctgcgggc
     1621 cggtgtccgc agtctcgtgc tctttcacca cgatcccgac cacgacgatc aaaagatcga
     1681 ctcgatggtg cagcaggctc gccagcaagt cgccgatgcc ggatcgcgct tgcaagtgca
     1741 tgccgcccgt gaacaagagc agctcgtgcc ccgcgcccgg caccaattag cggcttaaac
     1801 cgccgcgtcc atcgctctgc acgagtgcgg gtacgttttc cgctcacctc cctctcatgt
     1861 cgctgaagcg cattcttctt ctcagcggca tcgccacgct ctgttttgcc gccctctttt
     1921 tagaggatcg ctggcagcct ctcgtccttt tccggctgcg caacatggcg cgcgacgcgg
     1981 tcgcccgggc tggtcgcacc acgccgccta atcccgcgct ggttttcctc gcgatcgatt
     2041 ccgattcggt cggcctcgac gccacgaccg acgtcaaaca gctctatggt ttaagtgaca
     2101 agcagtcggt cgaagcgcac gccctggaat tgatgagcca ggcctggcca tggtcgcgcg
     2161 aagtttatgc tctcgtcctg cgccgcctcg tcgacgccgg cgcgaaagtg gtcgccttcg
     2221 acttgacgtt cccgacggcc actccggagg acccgccatt tcggctggcg ctcgatcgct
     2281 acaaggagca cgtcgtgatc gggagcaatt tcatctcgtc agcctcccgc ggctttgcga
     2341 ccgtggacgc gagcctcacg cgcccaccgg agtcgctcgt tccggaaacg cagccgatgg
     2401 atgatcgcgt cgccttcacc aatttttggc ccgaggaaga tgaagtggtt cggcaggcac
     2461 aattccggat cacctttgag caggtgcagg gcaatctgcc cggcccgaag accgagcgct
     2521 ttctttcctt cgccgcgcaa accttgatca aggccgggcg agcggatgcg gtgcctggcg
     2581 gattggacga gcgccttttc cgattcaccg cgccgccccg catcgggttc cggccgcatt
     2641 cgttgtttga aatttttgtg ccggattatt ggcagcacaa ttaccagtcc ggcgaattct
     2701 tccgtggcaa aatcgtggtc atcggggcgg aaggaaactg gcagcatgac gagcatccca
     2761 cgccgttcgg ttcgatgccg gggccggaac tgcacctcaa cgcgattaac gccgcgcttc
     2821 accatgagtt catctcggag atgtcgccgc tcgcggtcgt cagctttacg atcgcggcgg
     2881 gcctgctcgc gattttccta tctctcacca tccgctcgcc ctggctgcga ctgctggcac
     2941 tgatcgtcgg cgacgcaggc tgggcctggt tcgcgatttt tgtctttaac cacgcttcgg
     3001 tttacctccc gatcgccact ccctaccttc aactcaatct gactctgctc ctcggtctgg
     3061 ctgcggactt taccctcgaa cgagtagaga aaaaacgtgt ccgccaagct ttggagcgtt
     3121 atgtctcgcg cgacgtcgtc cgggaaatgc tcgataaacc gaagctctac gaacagtcgc
     3181 tcggcggcgt cttgaagcgg gttacgatcc tcttctccga cattcgcgga ttctccaaag
     3241 tcagcgctca gagcgaccca caggtcttgg tcaggcaact taacgaatac ctcaccgcga
     3301 tggtggaatg cgtttttcgt ttcggcggga cgcttgacaa gtttatgggt gacgcggtca
     3361 tggcggtctg gggaaatgtg cgcactgcgg gcgcgggcga cgatgccgtc gcggccgtcg
     3421 aggcggcgct cgcgatgcgc gcggagctgg cccggttaaa tcaaaaatgg cgggcggaag
     3481 gtttgccgga gctcaagatc ggcgtcgcgc tcaatcatgg cgcggtcgtg gccggcaaca
     3541 tcggttcgcc gcagcgcatg gagttcaccg tcattggcga cgcggtcaac gtgacttgga
     3601 agatgcagga actgaccaag agagtcggcg cggaattaat cgtgagcaaa accgtcgagg
     3661 cgctcatcgt ggagcatttc gagctgcacc cgctcgggca gttcgcgctc cataatttgc
     3721 cgggcgcctg ggaagtgttc gccatttccg agccgatcgc tgcgccgcgt gaacgagcgc
     3781 cgcgtccgct gcctcaaaac gcggctaagg aaacttgatc ggctcgaggc cgccggcttg
     3841 gagctgcgcg ttcaaggccg ccacctccgg cggaagcgcg cgccagcgct cgaccaccga
     3901 tcgcgcgtcg cgctgcagag cggccaccgc ggcttgttgc gccggagtcg gcaccccatc
     3961 gacaccctgc agatcgccga aaagtgtttc gaccttgcgc aggacatcca actcgagcgg
     4021 ttccccggga cgaacgtccg cgggattggt gaatccttcc agcttctggc gcagcgccgc
     4081 gaccgctccc cgggccgcgt ttttcccgag gcgttctttg gccttgccga gttccgccgc
     4141 cagcgccttg aaactccggc cgatgggctg gagggtgacg cgcgtttccg tcagggcttc
     4201 cgacagctcg aactgttttg ccagatccgc ggccgaggcc ttgacccgcg gatccatttt
     4261 cacgatcagc ggctgggtga aactcttgcc gcctgtcgtt agggcgaccg aataatttcc
     4321 gggcacgacc cagggcgcgg tcggttgcgg tgcggtttcg tgcgccaccg ccctcatcgg
     4381 gtattgcggc tcgacgcccg ccagcggctc gccgtgcaga tcccagaaga aacgatgcag
     4441 gcccggttcg gccgacaagg cctgcggcgg ccgcacccag tagcgcggga tcttcaattt
     4501 cggatccggc gtggggagcg ggtccgtgct cgcataacga cgcagcacct ttccctgccc
     4561 gtccttgatt tcgagcgtga ccgggccggc gacatctttg cctaacgaat aatcaatcat
     4621 cgcgccatcc ggcggatttt cgcccaccgg ttcatccggc gggaggggcg tatcggtgtt
     4681 gaaattccag cgcacccgca gcgcaatctc tggttggaaa agacacgttg ccttttcgtt
     4741 aggctggcgt tgccgcaacg gcgtgatgtt gtccatgatc cagaaaccgc gtccatgggt
     4801 cgcgatcgcg agatcgtcct ccttcacgat gaggtcgcgg atcgaagttg cgggcatgtt
     4861 caggcggagc gactgccagt tctcgccatc gtcgaacgag acgtaaaccg cccgctccgt
     4921 cccggcgaac aggagaccgc gccgctgcgg atcttcgcgc accgtgttca cgttttcgtt
     4981 aggcgaaatt ccctgcacga tctctttcca ggttttaccc gcgtcgctcg tccggtaaat
     5041 gtgcggccgg agatcatcga gccggatggt gttgaccgcc gcataggcgg tgttctggtc
     5101 gaaatgactc gcctccatca ccgaaacctt ccaccacgga ccgatctgcg acggtgtcac
     5161 gtcgttccag gttttgccgt cgtcggtcgt gaggtgaacc aagccgtcat ccgttcccgc
     5221 ccagatccga tttttgtcga gcggcgacgg cccgaccgcg tagatcacac cgcgctgcct
     5281 cggttgcgcg gtcggttggg aacgaaactt cccgacgctc gccggcactt cgaaggtcgc
     5341 gcgcgtcagg tcggggctga gctgggtcca atgctgcccg ccatcgcgcg tttgccagag
     5401 gtaattcgtg gcgaaaagaa gcaggtgcgg atcgagcggc gaaaaaagaa tcggttgcgt
     5461 ccggatcatg cgaaaatcgc tggtccgaaa cggctgcggc aggatgctct gcgcctggtg
     5521 ggtgcggcga tcgtagcgcg tcagtttccc gccgtagata atgtcgggat cgagcggatc
     5581 cgccacgaca tagccgtatt cctccgccgc caccggatgc caatcgcgaa aagtgatcgc
     5641 gccgtccggt ccgcggctcg tgatgccgac cgaaccgctt tcctgttgtc cgctgtaaag
     5701 gcgatagggg aacgcattgt ccgcgctcac gtgataaagc tgcgcggttg attggttgta
     5761 ccacgaactc caactcgcgc cgccgttcac cgtgatgatc gcaccctggt cgctcccgag
     5821 gagaacgatg ttcgagttgg ccggattgat ccagacgttc tgataatcat cgcccccggg
     5881 cgcaccgcgc cagccgtccc aggtcttgcc gccatcggtc gatttccagc agacgacgct
     5941 cgccgcataa acgacctttg gattttgcgg atcgattcgg accaagggca aatcgccgcc
     6001 gccgatccgc gcccccgggc gctcgtccgt cgtggccagg gtccagcttt cgcccgcatc
     6061 atcggaccga taaagcttcc cggcgtgcag cgtggcaacg gcggccacga gcgtttgcgg
     6121 agcgttcgcc gcgatcgcga tattggcctg gacgatatcg ttaggcagac ccttcgtcag
     6181 cctcttccag gtcttgccgc catcggtcga tttgaaaatc cctccgctgg ttccgttcca
     6241 ttccgcattt tcccacgggc cttcccgcgc ttcccaaagc gccgcgtaaa ccgtttccgg
     6301 attctgcggg tcaatctgca catccgccgc gccggtgttt tcatcctggt agagtgtttt
     6361 cgcaaaagtc ttcccgccat cgcgtgagag gaagatgccg cgctcctcgt taggcccgta
     6421 gggatgcccg gcgaccgcca cgagcacgtg atcagggttg tgcggatcga ccgcgatctg
     6481 cgcgatctgc tggccgtcgc gcaagccgag atgcgcccac gtcttgcccg tgtcagttga
     6541 tttataaatg ccatcgccga ccgaaagatc gggtcgatgg agcccttcgc cgctcccgac
     6601 atagattacc tcggggttgg aaactgaaac cgcgatcgcg cccaccgagc cggtcggttg
     6661 gtcatcaaaa atcggccgcc aggttcgccc gtaatcgttc gtcttgaaaa cgccgccatc
     6721 cacctgcgcg cagtaaaaga cattaggctg cgacgggacg cccgaaaccg cgcgcgtccg
     6781 gccgccgcga tacggcccga tgttgcgcca gcgcaggaga ttcgagaaat cctgctctgc
     6841 cgaagcgtat tcacccagag cgaggatcgg gagcaggaag aaaaggatgc ggaagcggct
     6901 tttcacgacg gcatgataag ctgctcggcg cgtcgcacca gcttccgggt ggcgcacagg
     6961 cctgcccgcc gtgtgcgggc atctggcgtg ctggtcgcgg cgttccgccg cgacgaattt
     7021 gcgctttccg gaggtgtggc ttctcagaaa ggttcgcgag cgtgggatac cgtcgccagc
     7081 actcgggacg agtgcgctac ccaggcgacg gcgccggcgt gggcgccggt tttttctttg
     7141 ccgcggcggc ggcgagcgcg tcatccacct gctcggcttc cagcgggtgg atgctgtgat
     7201 aataactgtt cgacttggcc aggtcgctct tgcgcatcag cagcgcgtcg aaacgctcgc
     7261 gccggctcag ctcgtaaacc ttgtccatct tgatcgcctc gaacggacaa acctcgacgc
     7321 agatctggca gctcatgcag accgagatat cgatgtcgaa ggtggccgga taaaactgtg
     7381 gtttgcccat gtaatcgggc ttcttgtccg cgcttttctc gatgtagatg cactgcggcg
     7441 ggcattcctt ctcgcagatc ttgcaggcga cgcagcgcaa cccggcctcc gggtcgttcg
     7501 tgtcgtaaat gaggaaggga aagttgcgcg agttttccgg gagcacgtcg cgctcctccg
     7561 gatattgcac cgtcgttagg cgatcttttt cgaagtaact cccgaggaag tttcgccccg
     7621 tcaccgccat gcccttgagg atccccgcgc caatcatcat cgatccactt ctcccatcac
     7681 aatatcgacg ctgccgagaa ttaccatcac gtcggccacg gtgtgcccga ggcagaggtc
     7741 ttccaggatc gtgaggttga tgaaactcgg cggacgcacc cggtagcggt acgggttccc
     7801 gctcccatcg ctgatcagat aaaatcccag ctcgcccttg ggcccttcga tccggccgta
     7861 ggcttcgccg agcggcggct tgaaggcgcg aagcttcgct ttcggattca tgatcggtcc
     7921 cgcgggcaaa tcgtccatcg cctggcgcag gaggccgagc gtctcacgca actcgagcac
     7981 gcgcatcatg agccgatcat aggtgtcgcc atgctcgccc agcggcaccc ggaatttgaa
     8041 gcgcggataa aatccgtagc cgtccacttt gcgaatgtcg taattcaccc cggaggcgcg
     8101 caacatgggg ccggttatac cagcgttgat cgccagttcg ggcgaaagtt tcccgacgtt
     8161 ctgggtgcgg gcgatgagga tttcgttgcc ggtgatgagc ctttcgaact cgtcgaggaa
     8221 gcgcgggaat ccatccacga ttttgcgcgc gcgtcccagc cagccggtcg gcaggtcaca
     8281 acggcaaccg ccgaaacgca tgtaatcaca catcatgcgc gagccggaaa gcgactcgaa
     8341 taggtcgaga atattctcgc gctcgcggaa ggcatacatc agcggcgtgc cccacgcgcc
     8401 catgtcgctg agcaaaaacc cgaggagcga agcgtgattc accaggcggg ttagctcggc
     8461 gaggatgatg cgcagatatt cggcgcgctc gggcacttcg atgccggcca gtctctcgac
     8521 gctgagcgcg tagccccagt tgttgctcat cgagcagatg taatcgagcc gatcggtata
     8581 cggcatcgag ccgaggtagc ttgtgttttc gccgatcttt tcgtgattac gatgcaggta
     8641 cccgaagaca ggcttgagct tgcgcaccat ctcgccttcg agcgcgacgt tcatccgaaa
     8701 gacgccgtgg gtcgacgggt gctggggacc cagcgaaact tcgagcagct cgccgtcgag
     8761 gcgcgaagtc ctgcgcggcg gcgcctgatc gagttcgtgg agaccggtcc cgctcatgtc
     8821 tgcgccgcga tgttttctgt gccgtcctgc gccggacgcg aaggataatg ctgcttcgcc
     8881 ttttccaaca cgtcgtcgtg cggcgtcggc tcatattcgt aatcgtcggg ttcccgataa
     8941 tccttgcgca ttggatgatc cgtaaattcg tcccacatca ggatgcggcg caggtcggga
     9001 tgcccgtcga aacggatgcc gtagagatcg tagatctcgc gttcctggaa ttccgcgctg
     9061 cgccagaccg gcgtgagcga tgggagcggc gtcccttcgg cacgatcgcg cgtccgcaac
     9121 ctaacgatga ccggtccgtg cttgtgcgcc atcgagtaga ggtgatagac cgcttccaag
     9181 tagccaggaa aaaattcctc ctgtgtctct tcgacttcct tttcctcacc ctcaacgagc
     9241 tttttcaccc ttaccttttt cttcgccgtc cgctcgagcc aatcgacgcc ggtcacgttg
     9301 gagcaataat ccagacgcag ggtcggatcg tcgcgcagga agcgcgcgac ggtggaggcg
     9361 tgcgcgtgat cgagcacgag cgacgactgg ttcgcggggc cgggattggg aatgatttcg
     9421 agacgggccc cgggtatcgc cgcttcaatg cggaatttta tttcgtcaag ggcttccatc
     9481 atgtcatccc gagcgccgtc gagggatccc gtggcgtgac gttaaagctg catcacggga
     9541 tccctcgact ccgctgcgct ccgctcggga tgacgtgctc atcaatgccg ctcgatcttc
     9601 ggcggatgaa acgtgtccgg gttcgccggc ggctccagat cgtgcgcccc gaaggccggc
     9661 accgggaact cgctcggcac cgttctgtcg aggaagtcgg ctttccccgt gccggtcagt
     9721 ttctgtccgt cgattttgcg ctgcaattcc atgaaggcgt ggaggagcgc ctccggccgt
     9781 ggcggacaac cagggatata aacatcgact gggatgtagc ggtcgatgcc tttcaagacg
     9841 ttgtagccct gcttaaacgg cccgccggaa atggcgcagg cgcccatcga gatgacgtac
     9901 ttcggatcgg gcatctggtt gtagagccta acgatctgtg gagccatctt cttggtcacc
     9961 gtgccagcga cgatcatgag gtcggcctgg cgcggcgagg gccggaagac ttcgccgccg
    10021 aagcgcgcca gatcgtagcg tgcggccgcg gtcgcgatca tctcgatcgc gcagcaggcg
    10081 aggccgaact ggagcggcca gatcgaattg cgccggcccc aattgtagag ctcctgcatc
    10141 gaggtcaccc agatgccttg tttttgcagc tcgctccgga gcccttgatc gattggcggc
    10201 gcgcttgcca ttacttcacc caacttaaac accctttgcc ccaggcccaa accagccctt
    10261 ccagcaggag aaggaggaaa accagaatcg cgaggaacgc gccgaagggc aacctggtaa
    10321 aggcgaccgc aaagggcacg aggaaaatcg cctcgacgtc gaaaatcaaa aagatgatcg
    10381 cgtaaagata atactgggag tggaactgaa tctgcgcctc gcctaacgat tcaacgccgc
    10441 attcgtaggt cgcattcttt cgcgcccccg gcttgggcgg ctgaaagaaa tgccgccaga
    10501 gccaggccag cgccaggggc aagagcggga agacgaaggc gaccgcgcca aaaatgacga
    10561 ggaagaaata aggagacggg gtcatggaat ggaaatgacg aatgttgaat gtcgaatgac
    10621 gaaggaattt cgaaacgcgc atgacgagag ctgcctgcag tcgttcgtca tttggtcatt
    10681 ccttcggatt tcgtcattcg agcttcgtca ttccggccgc cggccggtca aaccacgccc
    10741 tggtccatca ttgcgttcgc gactttgagg aagccggcga tgttcgcgcc attcacgtag
    10801 ttgccgggtg tgccgtaggt ttcggacgtt tggtaacagg cgcggtggat tgccttcatg
    10861 atggtttgca ggcggctgtc cacctcgtcg cgcgtccagg aaatgcgcat gctgttttgc
    10921 gccatctcga gaccggaggt ggcgacgcca ccggcgttcg cggctttgcc gggaccgtaa
    10981 agaattttgg cgtcgaggaa ctggttcacg ccttcgatcg tggtcggcat gttcgcgccc
    11041 tcggagatga cgtagacgcc gttgcgcagc aggttggcgg cgtccttgcc gttgatctcg
    11101 ttctgggtcg cgcccgggaa ggcgcactgc gccttgtgat tccaaagcgg gttgtgatcg
    11161 agattggggt cgaatgggat gtgaaccgcg cctttgaatt tctcggcgta ctcggcgatc
    11221 cggccgcggc gcacattctt gaggtccatg atgaagctga gcttctggtc gttgattccc
    11281 gcttcgtcgt agatgtaacc gctcgaatcg gacaccgtca cgggcttggc gcccatgttg
    11341 atcaacttct cgatcgtgta ttgcgcgaca ttgccgctcc ccgagatgag gcaggtcatg
    11401 ccttcgaggc cttccttgcg ggtcttgagc atctcggcgg cgaaatagac cgcgccgtag
    11461 ccagtcgcct gcgggcggat gagcgacccg ccccagctca ggcccttgcc ggtcaggacg
    11521 ccggtaaatt cgttacggag ttttttgtac atcccgaaga ggaaaccgat ctcgcggcct
    11581 ccgacgccga tgtcgcccgc cggcacgtcg gtgtaggcgc cgatgtggcg gaagagttcc
    11641 gccatgaacg actggcagaa gcgcatcact tcgttgtcgc ttttgccttt gggatcgaag
    11701 tcggagccgc ccttgccgcc gcccatcggc agcgtcgtca gggcattctt aaagacctgc
    11761 tcgaaggcga ggaatttgag gatgccgagg ttgaccgaac gatgaaagcg cagcccgcct
    11821 ttgtagggcc cgatcgcgct gttcatctcg atgcgaaagc cgcggttgat gtgaatctcg
    11881 cccttgtcgt cctcccacgg gacgcgaaac atgatcaggc gctccggctc gatcatgcgc
    11941 tcgaggatct tcgccttgcg gtattcggga tgacgctcga ggacgagcgc gagagactcg
    12001 acgacttcct ggaccgcctg gtgaaattcc ggttcggccg ggttcttggc cttcaccccg
    12061 gccatcaact ctgtcacgta atctttcata aatgattgag atctgtccgc cacttccgcc
    12121 cgagcctgcc gctctggcct aacgaatgat tagaaaaaca caaaagtctc gatctgtcgc
    12181 tgccgcgccc accggcgccg cgccatggcc tcgaacccga aaagatggag tcatcgcgca
    12241 tcaccgtatg ccggaggcgg tttaaaacag aagaacaact ttgtccagca aacggcgcga
    12301 accttccatc tggcccggaa gggatttcgt tccggtgcgc gcgcgggaag gcgattccgg
    12361 tcttgcttaa aaaagcagac ctcgctttct ttcatcgcaa cgtgagcggc tttctcgacc
    12421 agattttcat tcttgggaag cgatgggttc tctcgtttct tgccggcgca ccggactggg
    12481 tcggtcagat cgcttccagc ctgctcaaca tcatcgcgct gctcggcgtc tttctcagcc
    12541 tcttcgctct catgtcgctc ttcgagcgaa aaatcctcgc ccgcattcag aaccgctacg
    12601 ggcccaatcg cgtcggccct ttcggtctcc tccagccggt ggccgacggg atcaagatgc
    12661 tgatcaagga ggacatcgtg cccgcgcgcg ccgacaaggt cgtccatttt cttgccccca
    12721 tcctgatcgc cgccgcggcc atcctcgcgc tcggcatcat cccctacggc cgcaacatga
    12781 cgccgttcac aattgacggc ggcatcctct ttttcttcgc cgttggttcg acgaacgagg
    12841 tcggtgtttt catggccggc tgggggagca acaacaagtt ttccatgctc ggcgcgatgc
    12901 gcgcaatcgc gcagatggtg agctacgagc tgccgctcat catcaccgtc ctgccggtgg
    12961 tgatggtgac cggttcgctc acgcccgacg cgatcgtggc cgcgcagggc ggctactcgt
    13021 taggcgttct cccgcactgg ttcgttttca cgccctgggg cgcagctgcc ttcatcttgt
    13081 tttttgtctc ggggctggtg gaatcgaatc gcacaccttt cgacgtgccg gagggcgaat
    13141 cggaaatcgt cgccggccac atgactgaat actccggttt caaatacgcc atcttttttc
    13201 tcgcggaata ttttgggatg ttcgccgtca gcgggctggc ggtgacgctc tttctcggcg
    13261 gctggcacgc gccgctcagg gtcctggagt tcgttccgtc ctatctctgg tttttcctca
    13321 aactgggcgt gctcctcttc attttcatct ggattcgcgg gacgctgcca cgcgtccggg
    13381 tcgaccagat gatgagcttc gcctggaaat tcatgcttcc gatggcgttt acctgcattc
    13441 tcgccgccgc ggtctggcat tacgcgggcc atcgtctcgc cggctggttc ctgtcattgg
    13501 tcgtggtcct gctggtttac ctcgcgctct cgcgtcttct cggggccgga aaaaaatttg
    13561 ccccgcgcac ctatcgtttt gccgaatgaa cagcgccgcc ttctttctca tcgcggtgct
    13621 caccctcagc gccgccctcg ccgccgcctc ttttcgccag ctcgttcacg ctgcgctcag
    13681 tttcgcggtc gcgctggtcg gtgtcgccgc cttttacttt ttgttaggcg cggaattcgt
    13741 cggcctggtc cagatctttg tctacgtcgg cgcggtcgcg gtcctgatcg ttttcaccat
    13801 tttgctcacg caccgcgaac cggaggaaca tcgcggtttc aactggagcg gggcggtgat
    13861 cgcgcttgcc gtctttgccg gtttgctttg ggcgatcgtg cggacgcccg cgctttatgt
    13921 cgcggcgcaa ccggggacgg caatcaccgt ccggcaaatc ggtgaagcgc tcatgagcga
    13981 ctatgtctgg ccgctgcaat gcgtcggtct gctcctgacc gcggcgttga ttggcgccct
    14041 cgttctcgtc ctcgaggaga aacgatgatg caggcgcgac gcgcaattcc tggtccgggg
    14101 agcaggtgcg cctcgcacgc cggcgatggc gcccccgcct tcgcgaactt tttccccgat
    14161 ggtttcctgg cgaggcgttg gcgctcccca gtcccaattc caaccgcgcc atgaccccga
    14221 cgctcgccac cttcctcatc atctccgccg cgctcttttg cctcggcctg ctcgccgcgc
    14281 tcagccgccg gcacgcgatc ttcattctta tcgggatcga gatgatgctc gccgcggcgg
    14341 gattgaattt tatcgccttc tggcgtttcg gtccgcaacc cgcgccgccc accggtgtca
    14401 tggtgacgat cttcgcgatc gcgatcgcgg ccgcggaggc ggcggtcgga ctcgcgctcg
    14461 tcatcgcagt ctaccggcat tatcggacga cgagcgtcga ccggattgac cagcttaaag
    14521 gatgagcaac tggatcgtcg ttcatctttg gttgattccc gcggtgccgc tggcggcctc
    14581 gctcgtcatt ctcggcctgg cgaagtcccg ccgcgcggcg gccgccggac tcgccatcgc
    14641 cgggcaggtg gtcgcgctcc ttctctcgct cgacgctttc tggccgacgt tgcagacgcc
    14701 cggtgaccgc gtcttccaaa acttcacctg gttcaccttc ggcgaacaaa cgcttcggct
    14761 gggttttgtc ctcgacccgc tcgccgcggt catgctcgtc atgatcacgc tcgtcagtct
    14821 ctgcattttt gttttcagcg tcggctacat ggcggcggac aaaaatttca cccgcttctt
    14881 tgcctacctc tcgttttttt ccgccgcgat gctcggagtg gtcatcgcga acagtttgct
    14941 cctcctcttt gtctgctggg aattggtcgg gctcgcctct tatctcctga tcggtttctg
    15001 gattcacaaa ccgagcgcgg ccgccgcggc gaaaaaggcc ttcatcacca cgcggatcgg
    15061 cgacatcggg ttctttctcg ggattctctg gctctcctgg cggagcggca cgctgctctt
    15121 ttacgatggc ggaaacggcg cgctcgagag cgccggtctg ctctcgttag gcgcgagtgc
    15181 caccttcatc gccctcctca ttttctgcgg cgccgcgggg aagtcggggc aattcccgct
    15241 ccacgtctgg ttgcctgatg cgatggaagg tccgaccccg gtgagcgcgc tcatccatgc
    15301 cgccacgatg gtcgcggccg gcgtgtttct ggtcgcgcgg gtctaccccc tcttttcgtt
    15361 gggcgcgatc aacggggtca caccttcgct caccgtcgtc gtctggattg gcgtgatcac
    15421 cgcgctcatc tccgccctga tcgcgctcgc ccagccggac atcaagcgca tcctcgccta
    15481 ttcgactgtc tcgcagctcg gcctcatgat ggtgtcgtta ggcgtaggtg gagtggcggc
    15541 gggcatcatg catctgctcg cccacggttt tttcaaggct ctcctcttcc tcggctcggg
    15601 ttccgtcatt cacggctgcc acgaggaaca ggacatccgc cggatgggcg gcttgcgcgg
    15661 actcatgccg gtgaccttct tcacctacgc gatcggcatg ctggcgctga gcggcgtgcc
    15721 gctctttttt tccggcgcct ggacgaagga ggaaatcctg cacgcgacct cgcagtgggc
    15781 tccctcgcgg gtgccgcatt acctcatgct cgcgggcgtc atcctgaccg cgctctacat
    15841 gacccggcag atgatctatg tcttcttcgg cgagcggcgc gccgccgccg cgcgcgcgca
    15901 cgagagcccg ccggtcatga cgctgcccct gatcgtgctc gcgatctgcg cggtcggttt
    15961 cagcgtcgtg ctcactccgg cgtggccgtg gttgcacgat tatttgtctg gggagcgcgc
    16021 gacctacaat ctctcgctcc tcgttcagcc gatgcttttc gtctcgctcg ctctcgtggc
    16081 ggcgggagtt ggattgggaa tcgggttcta tcgctcggcc agcgagatcg atccgctcga
    16141 gcgcgcgctc cccgcgctct ttcgttttct cgaaaatcgg atgtggctgg atgaactcta
    16201 cgattggacg attctcagct gggcgcgatt cgccgcccgg ctctccgatt ttctcgatcg
    16261 ctatctttgg gatggcctcg tccggctggt cggcggattg ggccgggtcg gcggtggcct
    16321 aacgaaaggc ctggacgagc gcgggatcaa cgccggggtc gacgatgcca ccagggtgac
    16381 ccgtggtctc ggccgcttcg tctccgcggg ccactcgggc caggtccagg tttatctcag
    16441 tgtcatcgcg gtgggcatgc tcgcgcttct ccttctttac gcatggctca cctgatcagc
    16501 ggcttgattt tcattcctct ccttggggcg ctttttctca gcgtcgtccc atcgacgctg
    16561 gcgcgcccgg ctgcgctggg ctgcaatttc atcaccggct gtctcgccct ttcgatctgg
    16621 tatcacttcg acgctgccaa cccggcgttg caaatggttc agcggcacgt ctggattccg
    16681 gcgatcggcg cgcaggcgct cgccgggatc gacggcctga gcctcctcct cgtcctgctc
    16741 acttcgctga tttttccgtt cgccttttgc gccggccgca tgagccgcgg tttttattcc
    16801 atcatgctcg tcgcccaggc ggcgctctat ggcaccttca ccgcgcaaaa tttcattctc
    16861 tggttcctct tttacgagat gagcctgatt ccggccttcc tcctcatccg gatctggggc
    16921 ggcgaaaaac gcgaccgcgc ggcgaccaag tttttcctct atacctttct cggcagcgtc
    16981 gcgctcctgc tctcgtttct ttacatctac ttcgtcaggg gaacgtttga tttcgcggag
    17041 ctggcgtcgt taggcaagaa cgggctgctc gagggaaata ttcgctggct cgcctttgcg
    17101 ggcatttttc ttgggctcgc ggtcaaggtg ccgatttttc cttttcacac ctggctgccc
    17161 gacgcctacg aggctgcgcc gaccggggca tcgatggtcc tgaccggcct gctctcgaaa
    17221 atgggcgtct acggtttcat tcggcttctc ctgccgcttt ttcctaacga aatcaggatc
    17281 gccggtccgt ggctcctcgg gctcgcggtt tgctcgatcg ttttcgcctc gctcgcggcc
    17341 tgggcgcagt cggatctgaa acggatggtc gcctaccttt cgatcaatca tctcggctat
    17401 tgcatgctgg gcctcttcgc cgtgaccgcg acgacgtccg ccccgccggt cgaagcgcag
    17461 gccgccttga gcggcgtttt cctgcaaatc ttcaaccacg gcctgaccgc cgccgcgctc
    17521 ttctacttcg tcggactgct cgaggagcgg cgcggggcgc gcgggctcgg cgacttcggc
    17581 ggattgatgc aacggacgcc gctcctctgc gcctggatga gcgtggccat gttctcgtcg
    17641 ttaggcttgc ccggccttaa cggcttcgtc ggggaattcc tcatcttcaa gggctcgttt
    17701 gcgctcgccg ccggttacac cgcggtcgca gtcctcgggc tgctcttcac cgcgatcgtt
    17761 ttcctgcgcg cgatgcaggc cctcttcagc gggacgctcg cggaaagttg cagccagttt
    17821 cccgatcttc ggctcggaga aaagttggtc gtcgtcccgg ttacgctgct gatgttcgcg
    17881 gtcggcctct acccgcaatt cgttctccac atctttaact ccaccgtcgc gcaaatggcg
    17941 cggttgtcag gctgagtctt gcgatgagca aagagatcaa cgagttccga ttttcaaggt
    18001 cacgacctgt caccctgaag cgatgcgcgc attgtctttg cgctttcagc gaacggcgtg
    18061 tcgccatgct cccgggcgtc gcgcgtgcat ttgcagatgc tgcggagcgc acgcgactcg
    18121 cgtgctggcg atggcgaccc cgccgtcgcg aacttgctcc tagccgtcgg acatctgatt
    18181 tcggaagtat tgtcacgaca cgcttttcgg cgagtcgccg aaaagagcac gcgagtcgcg
    18241 tacgctcccc aatccgatga ttgcctgggc gatttacctc acttttgcgg gtgcgcttct
    18301 cgcgctcctt ctcccgcgca tcctggcgcg ttggattgcg ctcgtcacca cggcgggcgg
    18361 attcgccctc gggttggccg cgctcttcgc gacgaatgcc gatttcgccc acttcaccac
    18421 gatcgtgcgc gtgccctggg tgccggcgct cgggatgaat taccacctcg cggccgacgg
    18481 catcagtttg acgatgacgc tcgtgaccgg gctgagtgcg accgccgccg ttcttttttc
    18541 ctggaaggtc gaaaaccggc ctaacgaatt cttcttctgg ctgctgcttg tcgtggccgg
    18601 ctcctacggt gtttttttaa gcgccgatct ctttctcttc ttcgtcttct acgagctcgt
    18661 gatcgtgccg aaatatttcc tgatcgcgat ctggggttcg accaacaagg aatacggcgc
    18721 gatgaagctg acgctctact ccttcctcgg cggcgcgctg gttttcatcg gcatcctcgt
    18781 cgcctatgtg acggcgggaa cactcgattt gccgcagctc gcgcactttc aattcgctcc
    18841 gcagctccag gcctgggcgt tcccggtcct gtttctcggg ttcgccgttc tcggcggcat
    18901 ctggccgttg cacacctggg cgccgaccgg tcacgtcgcc gcgccgaccg ctggctcgat
    18961 gttgctcgcc gggatcgtga tgaagcttgg cgcttacggc gcgctccggg tcgcgctcaa
    19021 tctctttccg ttaggcttcc agatgtggag caaatggatt gcggtcttcg cggtcatcgg
    19081 gatcgtttac gcggcggcgg tcgcgctccg ccagagcgac ctcaaattcg tcattggcta
    19141 ctcgagcgtg agtcacatgg gctttgtcct gctcgggctg gccacggcca acgtgctcgg
    19201 cgtttcgggc gcggttttgc aaatgttctc gcacggcgtg atcggcgcgc ttctcttcgc
    19261 gctcgccggt gcggtttatt atcggacgca cacccgcaac ctgaacgacc tctccgggat
    19321 gggtctcagc cgggcgatgc cgtttctcac ctttgcgttc gttgtcgcgt cggccgcttc
    19381 catgggcatt ccgggtttca gcgggttcgc ggcggaaatc acgatcctga tcggcgcctg
    19441 gaaagctttt ccattggcgg tggggatcac tggccttggc atggtgctgg tcgcggcttt
    19501 caccctgcgc gcgttgaaga aatcgttctt cggcgagaac ccgaccggcc gcgtgtggcc
    19561 ggaaacgagc gcgccgagcg cggcggcgac cctggcggaa aagttgggcg ccagtctctt
    19621 gattggcgca acactcgcag ttggactttg tcccaatctc ctcctcgacc ggatcatgcc
    19681 ggcggtggag acgatgcggt ttttgcaccg atgagttacc tggaacttct tcagctggcc
    19741 gcgccggaag cgatcgttgt tgtgacggcg ctggtcgttt taacgatcgg gctcgtgggc
    19801 ggccggcagg aagcgggcga cacgctcgcc gccacacctg ttcttgggtc gctcgttgcg
    19861 gcgttcgggc tggcgatcgc ggcggccgcg atcgtcaggc tgccgggaca cgcgatgctc
    19921 tttcacggga tgctcgtgat ctcgccgctc aattcgcttt tccaggtgat cgtcctcgtg
    19981 ctggcgtttt ccaccgtcgt cctgacacgc ggcgaacatt ccttgcctaa caagagcgaa
    20041 tatctcgcaa tgatcctcct cgccacggtc gggctcatgc tcctcatcgg gagcgaagag
    20101 ctgctcatga ttttcatcgg gctcgagcta accgggctct cgctctacgt catgaccgcc
    20161 ttcgacaagg cgagtcagcc gtcggcggaa gccggcctga aatatttcct tttcggcagc
    20221 atggcgagtg cgttcaccct cttcgggctg agtttcatct atggcttcac cggcacgact
    20281 tcgctggccg cgatcgggcc gcgcctcgcc gagcaggcgc tgccgccgct ccttttcgtc
    20341 gggatcgtca tgaccctgat cgggttcttc ttcaaaatcg cggcggcgcc ttttcatctc
    20401 tgggcgccgg atgcctacca gggcgcgccg atctcgacgg ccgggttcat cgcttccgcc
    20461 tcgaaggtcg cgtcctttgt tgtcctcggg aaaatcctcc tcatcggttt cggttccgtg
    20521 cccggcagcg cggcctggca tgggatggtc gcgggctgga tgccgattct cgcggtcctt
    20581 tccgcgctct caattcttat cggcaacctc gtcgcgctcg tgcaaaaaaa tgttcgccgg
    20641 ctgctcgcct actcggcggt agcgcatggc ggttacactc tgctcgggct gatcgcgggc
    20701 gggcgcgccg gattttccgc gacgcttttc tacaccacga tctatgcggt cacgctcgtg
    20761 ggcgctttcg gcgttgttgg attcgttagg cgcgaaaatg gcggcgacga tctggccgat
    20821 ttcgccgggc tgcgggtgcg ttcgccactc ctcgccggct gcatggcggt cttcctcctc
    20881 tccctcgcgg gcctgccgcc gctcgccgga tttttcggca agttctacct cttcagcgtc
    20941 gctctgcggg ccggcgaaaa tgaaggcctg ctctggctcg tcgttctcgc gctcttcggc
    21001 agcctgattt cgctttatta ttacctcatc gtcctcaagg tcattttcct cgatgcgaac
    21061 gcgaagcccg cgccggcggt cgcgcgctac gattttgaag tgcgcgccga ttacctgtcg
    21121 aaagtggtgc tgctcgtgct cgccggcacc gtgctcttgc tcgggattat tccgggaacg
    21181 ctggtcgcgc ggatccttgc t
//
LOCUS       DATMNS010000225         6269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_5336707, whole genome shotgun sequence.
ACCESSION   DATMNS010000225 DATMNS010000000
VERSION     DATMNS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6269
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5336707"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VNW28_07900"
     CDS             <1..302
                     /locus_tag="VNW28_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HXA09891.1"
                     /translation="PLGREVEAGGEVGRHEILTHCYWREAGPEFRHVNVMAVAHGTDK
                     ALLLEHKAAIDRHLAAAGIPVSYTNVFWGGRSEIKPSEISPLIYRQWLAELGAEA"
     gene            complement(263..910)
                     /locus_tag="VNW28_07905"
     CDS             complement(263..910)
                     /locus_tag="VNW28_07905"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HXA09892.1"
                     /translation="MSRPDRLIAADLRASLGEGVIGRRIIVLESTASTNDFLRQMLTP
                     ELPEGFVVFAEEQTAGRGQRGNRWASASHLGLWFSLLLRPILSLPESVCLTNWAAQAI
                     ATAVRNETGLEPAIKLPNDVYLSDRKIAGVLVETKARAGAQCDAIVGLGINLNQARED
                     FPAELQERAGSIAMALGRKIDRSDFAIALLRELERTRAAALSLQASAPSSASHCR"
     gene            complement(897..1751)
                     /gene="nadC"
                     /locus_tag="VNW28_07910"
     CDS             complement(897..1751)
                     /gene="nadC"
                     /locus_tag="VNW28_07910"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HXA09893.1"
                     /translation="MNFSDEDYQLIDLALREDIGSGDVTSQYFVDETDQASARIIAKE
                     RAIVAGADVAAEVFRRVDPVLRIEVLQPDGAAVAGGVAVIEIRGLARSILTAERVALN
                     FLQHLSGIATLAHQFVEAAGATGAKILDTRKTTPGLRKFEKAAVVAGGGANHRFGLYD
                     MVLVKDNHLLVRTGLARLGAAIERVRQERPGLRIEVEADNLDQVRALLEVKGVEVVLL
                     DNMSASQMREAVAIGKGKVKFEASGGVNIRNVRQIAATGVDFVSIGALTHSARALDFS
                     LELTHVAA"
     gene            complement(2065..2973)
                     /locus_tag="VNW28_07915"
     CDS             complement(2065..2973)
                     /locus_tag="VNW28_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HXA09894.1"
                     /translation="MRLLLVDGHYYVYRSYFAILHLSNSRGEPTNAIYGFTKSLRLML
                     KHLQPAFGAVVWDRGVPQRRTELQPGYKETRKEMPPPMVPQLDYIQQLTPWLGFQNIS
                     LPDTEADDLMGCYAVEARARGIEVVLATNDKDLYQLVNSTVKIYTTAKGDLASPKDAF
                     ALLGEEEVMAKWEVPPPLIGDVLAIAGDAVDNIPGVGIGRKAAAKLLREFGSLDALLR
                     GLDRIKTPKTREKLETARERVLQNRKMVALDCHLPLPVPIDALRITPDYPRLIASLEK
                     NEFKSLLQDVRDEAARCGTQTQGELL"
     gene            3169..4272
                     /locus_tag="VNW28_07920"
     CDS             3169..4272
                     /locus_tag="VNW28_07920"
                     /inference="COORDINATES: protein motif:HMM:NF025597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HXA09895.1"
                     /translation="MADQWIVRVQGREYGPVDLDELREWKREGRLIRENEIREPDSER
                     WIQAGELPEIFSDEAAPPGPEEVVRLLAFGEIFATGWRVYRQGFGHFFALALLVSAPA
                     FLLQIAVPYLEMPKDQGSVAPVIISVAVAFAMLVLLVITWPFSLACMQLLAADLCAGR
                     SPTLRELLTRAKPLWTRMFTLGLIVYGSYILWAILPIIVAAGLAAGATTFGSLLLMLL
                     LLIFALYMVARLFINFLFWQQAGALGGRDALGALRESKDLARGGTDRPRRQRPLYRGA
                     MIASLWLLVIIILNAGIELPVVLFRMRGVTTPEQAVTMLQTMATTNSLDFVSGLTTFL
                     SGLLHAALRGWLAAIFVVLYFDTKRTAPPPPNE"
     gene            4299..4772
                     /locus_tag="VNW28_07925"
     CDS             4299..4772
                     /locus_tag="VNW28_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09896.1"
                     /translation="MPPLDPSFAFSFVVPKLQLGNALVFEALLHQSRYYAQPRSRARN
                     AAHALHHPDNRRVASGVQLRCLLRHRGALPQVLQRKEGAENSRLVILDSHLHAVVAAP
                     ALSAVLRDLKSFTAQQILAQVGRDWLLNPLAYYRAAHKQTAHQVWQGGASAGNHE"
     gene            complement(5020..>6269)
                     /locus_tag="VNW28_07930"
     CDS             complement(5020..>6269)
                     /locus_tag="VNW28_07930"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXA09897.1"
                     /translation="VQAARRAHRNVWSKMPGRERGKFLYRIARLVQEKARELAVLESM
                     DGGKTIKESRDIDLPLVAAHFFYYAGWADKLEYAFPGRTPHSLGVAGQIIPWNFPLLM
                     AAWKLAPALAAGNTCVLKPAETTSITALHLAEIFQEAELPDGVVNIITGAGSVGKALV
                     EHDDVNKIAFTGSTAVGKMLTRAVAGTKKSLTLELGGKAANIIFEDAAIDQAVEGIVA
                     GIYFNQGHLCCAGSRLLVQEGVFSAVVRKLRDRISSLRVGNPLDKNTDIGAINSRMQL
                     DKICALVESGLQQGAELVQPRLRLPAKGYWYPPSFFTGVTASHRIAQEEIFGPVLSVM
                     TFRTPEEAIERANNTPYGLSAGVWTDKGSKIFKIVSRLRAGVVWANTYNKFDPTSPFG
                     GFKESGFGREGGLHGLRPYLAFG"
BASE COUNT         1160 a         1950 c         1909 g         1250 t
ORIGIN      
        1 tgccgttagg ccgcgaggtg gaagccggtg gcgaggtcgg gcggcatgag attttgaccc
       61 attgttattg gcgcgaagcc gggccggaat ttcgccacgt caacgtcatg gcggtcgcgc
      121 acgggacgga caaggcgctc ctcctcgagc acaaggccgc gatcgaccgg cacctcgcgg
      181 cagccggcat tccggtttcc tacacgaatg ttttctgggg tgggcggagt gagatcaagc
      241 cgtctgaaat ttcgccgctg atttaccggc aatggctggc ggagctcggc gctgaggctt
      301 gaagcgagag ggccgcggcc cgggtccgtt ccaattcacg gaggagcgcg atcgcaaagt
      361 cggaccggtc aatttttcgc cccagggcca tggcgattga tccggcgcgc tcctgtaact
      421 cggccggaaa atcttcccga gcctggttca aattgatgcc cagcccgaca atggcgtcgc
      481 actgtgcgcc cgccctggct ttcgtctcga ccaggactcc ggcaattttt cgatcgctca
      541 gatagacgtc gttaggcaat ttgatcgccg gctcgaggcc ggtctcattg cggaccgcgg
      601 tcgcgatcgc ttgcgccgcc caattcgtta ggcagaccga ctcggggagc gagaggatcg
      661 gccgaagcag aagcgaaaac cacaggccga gatgggacgc cgaggcccag cgatttccgc
      721 gctgaccgcg gccggcggtc tgctcttcgg caaacacgac gaagccctcg ggcaactccg
      781 gggtgagcat ctggcggagg aaatcattcg tcgaggcggt cgattccagc acgatgatgc
      841 gccggccgat gacaccttcc cctaacgacg ccctaaggtc ggccgcaatc agccggtcag
      901 gccgcgacat gcgtcagctc cagcgaaaaa tcgagcgcgc gcgcggaatg agtaagtgcg
      961 ccgatcgaga caaaatcgac gccggtcgcg gcgatttgcc ggacattgcg gatgttcacg
     1021 ccgccgctcg cttcgaactt gaccttccct ttgccgatcg cgacggcttc gcgcatctgg
     1081 ctggcactca tgttgtcgag caggaccacc tccacgccct tgacttcgag gagcgcgcgc
     1141 acctggtcga ggttgtcggc ctccacttcg atccgcaggc cgggccgctc ctgccgcacc
     1201 cgttcgattg cggcgcccag tcgggcgagg ccggtgcgca cgaggaggtg gttgtctttc
     1261 accaacacca tgtcgtaaag cccgaagcga tgattcgccc cgccgcccgc cacgaccgct
     1321 gctttctcaa atttccgcaa gcccggtgtg gttttgcgcg tatcgagaat ttttgcgccc
     1381 gtggcgccgg ccgcttcgac gaattggtgg gccaaggtcg cgatcccgct aaggtgttgc
     1441 agaaaattga gcgcgacccg ttcggcggtg agaatcgagc gcgcgaggcc gcggatttcg
     1501 atcacggcca cgccgccggc gacagcggcc ccatctggtt ggaggacttc aatgcgcaag
     1561 accgggtcga cccgacgaaa aacctcggcc gccacgtccg cgcctgcgac gatagcccgc
     1621 tctttcgcaa tgatgcgcgc ggaagcctgg tccgtttcgt ccacaaaata ctggctggtg
     1681 acgtcgccgc taccgatgtc ctcgcggagg gccaggtcaa tcaactggta gtcttcgtcg
     1741 gaaaaattca taccccggaa agcctgcggc attgaagggc gcggcggcgc gtcccaacag
     1801 atgcacaatg gggaaagaat gccgggcaac aagcccgccg agacaaaaaa aagatccggg
     1861 cagggcacgc cgcggagaaa ccttcgaatg cgagggcgtg ccaccgcccg aatcgcctta
     1921 accagtgaag atagacagcg gggctcgggg cgcgttcaaa agatttccgg tagaagctgc
     1981 cctcagggcg acgccggggt gcctcccgcc cgcatggttc gattttggtc cacgctcaca
     2041 ctcgcgcttc gccgagcgaa gcgtctacaa gagttcgccc tgcgtttgcg ttccgcagcg
     2101 agccgcctcg tcgcgcacat cctgcaggag cgatttgaat tcgtttttct ccagcgatgc
     2161 aatcaagcgc gggtaatcgg gcgtgatgcg caacgcgtcg atcggcaccg gcagcggcag
     2221 atgacagtcc aacgcgacca ttttgcggtt ttgcaggacg cgctcgcgcg ccgtctccag
     2281 tttctcgcgc gttttcggag tcttgatccg gtccaggccg cgcaagagag cgtcgagact
     2341 cccgaactcg cgcagcaact tggccgcggc tttgcggccg atcccgaccc cggggatgtt
     2401 gtcgacggca tccccggcga tggcgaggac gtcgccgatg agcggcggcg ggacctccca
     2461 tttcgccatc acctcttctt cgcctaacaa tgcgaaggca tccttcggcg aagcgagatc
     2521 gcccttcgcg gtcgtgtaga ttttcacggt cgaattgacg agctgataga gatctttgtc
     2581 gttcgtcgcg agcacgacct cgattcctcg ggcgcgcgcc tcgaccgcgt agcagcccat
     2641 caaatcgtcc gcttccgtgt ccggcagcga aatgttctgg aagccgagcc agggcgtgag
     2701 ctgctggatg tagtcgagct gcgggaccat gggtggcggc atttccttgc gcgtttcctt
     2761 gtaaccgggc tgtagttccg tccggcgttg aggaactccc cgatcccaga cgacggcgcc
     2821 gaacgcgggc tgcaaatgtt tcaacatgag ccggagcgat ttggtgaacc catagatcgc
     2881 gttggtcggt tcaccgcggg aattagagag atgcaggatc gcgaaatagg agcggtaaac
     2941 gtaatagtgg ccgtcgacta gcaggagtct cacccggtta ccatatcgca ggcgggtgag
     3001 aaatgactaa ttccgaatga cgaaacacca aatgacgaac gcagcggcct gcacgctccg
     3061 actctcggac ttcgtcattc ggaattcctt tgtccttcgt ccttcggaat tcgtcattca
     3121 tcattcctca ccctagtcat tccccgaaat cgcgattaag tgagcccgat ggcagaccaa
     3181 tggatcgtgc gggtgcaggg ccgggaatac ggcccagtcg acctcgacga actgcgcgaa
     3241 tggaagcgcg aagggcggtt gatccgcgag aatgaaatcc gcgagccgga cagcgagcgc
     3301 tggattcagg ccggcgagtt gccggaaatt ttctcggatg aagcggctcc gcccgggccg
     3361 gaggaagtcg ttaggctgct cgcctttggc gaaatctttg ccaccggctg gcgggtttac
     3421 cggcagggat tcgggcattt tttcgccctc gccctgctcg tctcggcccc cgctttttta
     3481 ctccaaatcg cagtgcccta cctcgagatg ccaaaagacc aggggtcggt cgcgccggtt
     3541 atcatcagcg tggcggtcgc gttcgccatg ctggtgctgc tcgtgattac ctggcccttt
     3601 tcccttgcct gtatgcagtt gctggcggcg gatctctgcg ccgggcgcag cccgaccctg
     3661 cgcgaactgc tcacccgcgc caaaccgctc tggactcgga tgtttaccct cgggctgatc
     3721 gtttacggca gttatattct ttgggcgatc cttcccatca tcgtggcggc ggggctcgcg
     3781 gcgggcgcga ctacgttcgg cagcctgctg ctgatgctgc tcctcctgat tttcgcgctc
     3841 tacatggtcg cgcgactttt cattaacttc ctcttctggc aacaagccgg cgcgctcgga
     3901 ggacgggacg cgctcggggc attgcgtgaa agcaaggacc tggcacgcgg cggaacggat
     3961 cggccgcgca ggcaacgacc gctttatcgc ggggccatga ttgcatcgct ctggctgctc
     4021 gtgattatca ttttgaacgc cggcatcgag ctaccggtgg ttctctttcg gatgcgcggc
     4081 gtgaccaccc cggagcaagc cgtgacaatg ctccagacga tggcgacaac gaattcgctc
     4141 gattttgttt ccggcctaac gacttttttg agcggcctgc tgcacgcggc cttgcggggt
     4201 tggctcgcgg cgatcttcgt tgttctttat ttcgacacga agagaaccgc gccacccccg
     4261 cctaacgagt agcaaaggct cagatccggc cgcaagccgt gccgccgctc gatccctcgt
     4321 tcgctttctc gttcgtcgtt cccaagctcc agcttgggaa cgcacttgtc tttgaagctc
     4381 tgcttcatca gtctcggtac tatgcgcagc cgcgatcgcg tgcacgaaac gcagcacacg
     4441 cacttcatca ccccgacaat cgtcgagtgg cttccggtgt tcagctccgc tgcttgttgc
     4501 gacatcgtgg tgcgctccct caagttttgc agcgaaaaga aggggctgaa aattcacggc
     4561 tggtcattct cgacagtcac ctccatgcag ttgtcgctgc gccggcgttg tccgcggtct
     4621 tgcgcgacct gaagagtttt accgcgcagc agattctcgc gcaggtgggc cgcgactggc
     4681 tgctcaaccc gctggcttat tatcgcgccg cgcataagca gacagcccac caagtctggc
     4741 aggggggcgc atccgcaggc aatcatgagt gatgccatga tggagcaaga gctgagtatt
     4801 tgcacaataa tccggtgaaa gccgcgctgg tggcttcacc ggaacattgg cgctattcct
     4861 ccgcgcacca gtggctgccg ggggcgcaaa cggtgttgcg gtgcgatggg tggcgctaga
     4921 ggaagaagct ggatcttcca agccgaccgc gttcccaagc tggagcttgg gaacgaggtt
     4981 ttcgatgacc aaatagccaa gaagacgccc gcctaacgac taaccgaacg ccagatacgg
     5041 ccgcaaaccg tgcagcccgc cctcgcgacc gaagccgctc tccttgaagc cgccaaacgg
     5101 actggtcggg tcaaacttgt tgtaggtgtt cgcccagacg acgcccgccc gcagccggct
     5161 cacgattttg aaaatcttgc ttcccttgtc ggtccaaacg ccggcgctca agccgtaagg
     5221 tgtgttgttc gcgcgctcga ttgcctcctc cggtgtacgg aatgtcatta cgcttaagac
     5281 cggtccgaat atttcctctt gcgcgatccg atgcgaggcg gtgacgccgg tgaaaaaact
     5341 cggcggatac cagtagccct tcgcgggcag gcggagccgt ggctggacca actctgcgcc
     5401 ttgttgcaac ccgctctcga ccagtgcgca gatcttatcg agctgcatcc gcgaattgat
     5461 cgcgccaatg tcggtattct tgtcgagcgg gtttcccacg cggagcgagg aaatccggtc
     5521 gcgcaacttg cgaacgaccg ctgaaaaaac gccctcctgc acgagcaaac gcgaacccgc
     5581 gcagcagagg tgcccctggt tgaaatagat gcctgcgaca attccttcaa ctgcctggtc
     5641 gatcgccgcg tcctcgaaga tgatgttcgc cgccttgccg ccgagctcca gggtgaggct
     5701 tttcttcgtc ccggcaaccg cgcgtgtcag cattttgccg accgcggtcg aaccggtaaa
     5761 cgcaatttta ttgacgtcgt cgtgctcgac cagcgcctta ccgacagagc cggcgccagt
     5821 gatgatattg acgacaccat cgggcagctc cgcttcctga aaaatctccg ccagatgcag
     5881 tgcggtgata ctggtcgtct cggctggttt gagcacgcag gtgtttccgg ccgccagcgc
     5941 cggagcgagc ttccacgccg ccatcaggag ggggaagttc cagggaataa tctgccccgc
     6001 cacgcctaac gagtgcggcg tgcgtccggg aaaagcgtac tcgagtttat ccgcccagcc
     6061 cgcgtagtaa aaaaaatggg cggccaccag cggcagatcg atgtcgcggc tttccttgat
     6121 cgtcttgcca ccgtccatcg actcgaggac ggcgagctcc ctggcttttt cctgcacgag
     6181 gcgcgcgatg cggtagagga atttcccccg ctcgcggccc ggcattttac tccagacgtt
     6241 gcggtgcgcg cgccgcgccg cctgcaccg
//
LOCUS       DATMNS010000226         3510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_677648, whole genome shotgun sequence.
ACCESSION   DATMNS010000226 DATMNS010000000
VERSION     DATMNS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_677648"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="VNW28_07935"
     CDS             <1..1051
                     /locus_tag="VNW28_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HXA09898.1"
                     /translation="DASGRAAFLARKLGYFCQNSEHPINAAWARFTGVKDWDSYEWRQ
                     RFPDYAGAARTGRGWATNHLMGQGWWCWIIPLKGGDFSAGIVYDSRLFTLPEGANLGE
                     RLHQHILQHPVGREIFADAKIIPGDVRAYSALPYHSTNVCGDGWALVGDAAGFLDPLY
                     SPGLDLCAYTTSLVSDLLVRALGGEDVAGRLKYYNEQFPTTYRCWFETLYQDKYYYMG
                     EADLMSAALLLDVGLYFVGLVRPIYADPEGAFLQLPFEGASGRLFAALMKFYNRRLAA
                     LAKNRIAHGVTGVNNAGWRELYDGFVPDFRLQKLIRKGLFRWWKAELRNLRFLFASPK
                     PTPRATAPAAATVEA"
     gene            1051..1956
                     /locus_tag="VNW28_07940"
     CDS             1051..1956
                     /locus_tag="VNW28_07940"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXA09899.1"
                     /translation="MFAFWRRRFAVRGVLWRHYLDFGLTNVPFYLQPVMIFFWTIFFF
                     FLAAPARRAILSNLRVVLPGSSPLLNLGRAWLTLYNFAWTISDAANDRLAQTEFAYEM
                     EGAEFLDQLAAAQGAVLLTAHMGNYDLGAAIFARRFRREIRMVRAPESDEQTAQHLGA
                     SLERAGAGAVKVAYNTSSMALPFELLNAIRQGEIVSIQGDRAVEPGSQRAAQLFGQVV
                     RLPDGPFMLAFVTEAPIFPLFIVRASYHRYKIIARAPIYCRSTGRGREPALEAAMEAW
                     CRTLESVVAAHWSQWFSLVPIFPAR"
     gene            1953..2435
                     /locus_tag="VNW28_07945"
     CDS             1953..2435
                     /locus_tag="VNW28_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09900.1"
                     /translation="MNDPATREIGYAFALPRLVARLGGRKVRRAELSRGEAYGVGWLV
                     FGIACVCAARALLPFIRPLPGQLLVALLLPFATWIAFLLLYFLNWLIVAFLRRLDLYS
                     GRTNNSFQHFVIISLITFLALRLAQDESGWLKSLGMLWLGLVGLNLLALLVLKLRHES
                     "
     gene            2425..3201
                     /locus_tag="VNW28_07950"
     CDS             2425..3201
                     /locus_tag="VNW28_07950"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXA09901.1"
                     /translation="MKVEWRRQLILGFALVAALFFLAVFFFHPWLALGALFLSHLLIL
                     YPTLLANCQWWGPVVTHFETPRREVWLTLDDGPDPIHTPRMLELLRRAGAQATFFVVG
                     QRATKFPAEMGAIRAAGHEIANHTFSHPSGTFWCLPPGRIATEIDRCDFSARYFRAPA
                     GLKNFFVHPALARRNLQPIGWTVRGFDTVSRDPAAVAARIMQGVKPGVILLLHEGHRT
                     ATDPDFHPCCLELVLLALTKANYRCVLPGPDQLRSRAGEK"
     gene            3206..>3510
                     /locus_tag="VNW28_07955"
     CDS             3206..>3510
                     /locus_tag="VNW28_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09902.1"
                     /translation="MIVERRAPPERLHGAGELISVEGGQDFLAGRKMERHLDVPGRHL
                     GETFGQMSEPVTRVAVARTIADDEQAARRDARREPGEEPRLFVRRKIMQDVEEDDVTV
                     "
BASE COUNT          536 a         1137 c         1110 g          727 t
ORIGIN      
        1 ggacgcgagc ggccgggccg cctttctcgc gcgcaaactc ggctattttt gccagaattc
       61 cgagcatccg atcaacgctg cctgggcccg ttttaccggg gtgaaagatt gggacagcta
      121 tgagtggcgg cagaggtttc cggattacgc cggtgccgcg cgcaccggcc gcggctgggc
      181 gacgaatcat ctcatgggcc agggctggtg gtgctggatc atcccgctga agggcggcga
      241 tttcagcgcg gggatcgttt acgacagccg tttgttcact ttgcccgagg gcgcgaatct
      301 cggcgaacgg ttgcaccagc acatccttca gcatccggtc ggccgcgaga ttttcgcgga
      361 cgcgaagatc attcccggcg atgtccgggc ttactccgcg ttgccgtatc acagcacgaa
      421 tgtttgcggc gatggctggg cgctggtcgg cgacgcggcc ggattcctcg atccgcttta
      481 cagtcccggc ctggatctct gcgcctacac cacttccctg gtgagcgacc tgttggtgcg
      541 cgcgctcggc ggcgaagacg tggcgggccg gctgaagtat tacaacgaac aattcccgac
      601 gacttaccgc tgctggttcg agacgctcta ccaggacaaa tactattaca tgggcgaggc
      661 ggacctgatg tcggccgcgt tgctcctcga cgtgggcctt tatttcgtgg ggctcgttag
      721 gccgatctac gccgacccgg aaggcgcctt cctccagttg ccgttcgaag gcgcgtccgg
      781 ccggctcttc gccgcgctga tgaagtttta taatcggcgg ctggcggcgc tggcgaaaaa
      841 ccggatcgcg catggcgtca cgggagtgaa caatgccggc tggcgcgagc tctacgacgg
      901 attcgtgcct gattttcggc tccaaaaatt gatccgcaaa ggtctctttc gctggtggaa
      961 ggcagagctg cgcaatctgc gttttctctt cgccagcccg aaaccaactc cgcgcgcgac
     1021 cgcgccggcg gcggccaccg tcgaagcctg atgttcgctt tctggcggcg ccgtttcgcg
     1081 gtgcgcggtg ttctctggcg gcactatctc gattttggcc taacgaacgt cccgttctac
     1141 ctccagccgg tgatgatctt cttctggacg atcttctttt tcttcctcgc cgcgccggcc
     1201 cggcgcgcca ttctgagcaa cctgcgggtc gtcctccccg gctccagccc gctgctcaat
     1261 ctcgggcgcg cctggctgac cctttacaat tttgcctgga cgatttccga cgcggccaat
     1321 gaccggctgg cgcaaaccga gttcgcctac gaaatggagg gagcggagtt tctcgaccag
     1381 ctcgcggcgg cccagggcgc ggttttgctc accgcccaca tgggaaacta cgatctcggg
     1441 gccgccattt tcgcgcggcg tttccggcgc gagatccgga tggtccgcgc gccggaatcc
     1501 gacgaacaaa ccgcgcaaca tctcggcgcc tcgctcgaac gcgcgggggc gggcgcggtc
     1561 aaggtggctt acaacacctc gagcatggcg ctccccttcg agctcctgaa cgccatccgg
     1621 cagggggaga tcgtttccat ccagggcgat cgcgcggtcg agcccgggtc gcagcgcgcg
     1681 gcgcagctct ttggtcaggt cgttaggctg ccagacgggc ctttcatgct cgccttcgtc
     1741 acagaggccc cgatttttcc gctcttcatc gtgcgggcgt cgtatcaccg ctacaagatc
     1801 atcgcccgcg cgccgatcta ttgccggagc accgggcgcg ggcgcgagcc cgcgctcgag
     1861 gcggcgatgg aggcgtggtg ccgcaccctc gagagcgtgg tcgccgcgca ctggtcgcaa
     1921 tggttttcgc tcgttccgat ctttcccgcg cgatgaatga cccagccacg cgcgagatcg
     1981 gttacgcctt tgccctgccg cggctcgtcg cgcggctcgg cggacgcaaa gtgcgccggg
     2041 ccgaactcag ccgcggggaa gcttacggag tcggctggct cgtcttcggg atcgcctgcg
     2101 tctgcgccgc gcgggccctt cttcctttca tcaggccact ccccgggcaa ctgctggtcg
     2161 ccctgctcct gccgtttgcg acctggatcg cgtttctcct cctctacttc ctcaactggc
     2221 tgatcgtcgc gttcctgcgt cgactcgacc tctactccgg acggacgaat aattcgttcc
     2281 agcatttcgt catcatctcg ctgattacgt ttctcgcact ccgcctcgcg caggacgaaa
     2341 gcggctggct gaaatcgtta ggcatgctct ggctcggact ggtcggcctg aatcttctcg
     2401 ccctcctcgt gctgaaactt cggcatgaaa gttgagtggc gccgccaatt gatccttggt
     2461 ttcgcgctgg tcgcggcgct cttctttctg gccgtcttct tcttccaccc gtggctcgcg
     2521 ctgggcgctc ttttcctttc gcacctgctc atcctctacc cgacgttgct cgcgaactgc
     2581 cagtggtggg gacctgtggt cacgcatttc gagacgccgc gccgcgaggt ctggctcacg
     2641 ctcgacgacg gtccggatcc gattcacacc ccgcggatgc ttgaacttct gcgccgcgcc
     2701 ggcgcgcagg cgaccttttt cgtcgtcggc cagcgcgcga caaagttccc ggcggaaatg
     2761 ggagcgatcc gggccgctgg tcatgaaatc gcgaaccaca cgttctcgca tccgagcgga
     2821 acgttctggt gccttccgcc cgggcgcatc gccacggaga tcgatcggtg cgacttctcc
     2881 gcccgttatt ttcgcgcgcc ggccggcctg aaaaatttct tcgttcatcc cgcgctcgcc
     2941 cggcgcaacc tgcaaccgat cggctggacc gtgcgcggat ttgatacggt gagccgggat
     3001 ccggcggcgg tcgcggcccg gatcatgcag ggggtgaagc cgggcgtgat tctccttctg
     3061 cacgaagggc atcgcacggc gaccgatccg gattttcatc cgtgctgtct cgagcttgtg
     3121 ctgctcgccc taacgaaagc gaattatcgc tgcgtcctgc ctggcccgga ccagctgcgg
     3181 tcgcgcgccg gcgaaaaatg aagaggtgat tgttgaacgg cgagcgcccc cagagcggct
     3241 gcacggtgcg ggagaactga tcagcgtcga aggcggccag gattttctcg cgggtcggaa
     3301 aatggagcgg cacttggatg ttccaggtcg tcatctgggc gaaacgttcg gccagatgag
     3361 tgagccagta acgcgcgttg cggtcgcgcg gacaatcgcg gatgacgagc aggccgcccg
     3421 gcgcgacgcg cggcgcgagc cgggcgagga gccgcgcttg ttcgttaggc gaaagataat
     3481 gcaggacgtc gaagaggacg acgttaccgt
//
LOCUS       DATMNS010000227        10017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2838154, whole genome shotgun sequence.
ACCESSION   DATMNS010000227 DATMNS010000000
VERSION     DATMNS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 10017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10017
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2838154"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VNW28_07960"
     CDS             <1..1122
                     /locus_tag="VNW28_07960"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXA09903.1"
                     /translation="YIQANFEDGDVSAFEGRFGLSALKDRFRLRRARINLSGDFAEQF
                     DFKVEGDFEQSDSAITALKSVSTTTGKTTTVSNATRTEFSATDVFINWHGIPEANIKV
                     GQWKAPFGLEQLTPDPKLFIIERSLPTGALTPERQVGVQVWGKPLTNLWPQETDLVTY
                     YAGVFNGNGRNVNTNDNNNFMYVGRLELLPFKGKVAGQEVSLKLGGDVLNSRDDAGTI
                     ISPSGNLKVNTDGSLAAYTLPGAAERTAWSVDAWLNIGPFDLIGEYLSEDVNGRTVNG
                     VAPGFTDFSPSGWYVQGSYFIIPKKFQAAVRWEALNPGQLGQDGIHSITGGLNYYIHG
                     DALKVMANYVHTWSDFRAANPTFGDDQFDEVILRLQLMF"
     gene            1222..2205
                     /locus_tag="VNW28_07965"
     CDS             1222..2205
                     /locus_tag="VNW28_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR01728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXA09904.1"
                     /translation="MKIKPMKLHTLAALIGAAILCATSLNAEEKTTIRFGHFPNVTHA
                     QGVIAHALSRQGKGWFEQRLGPNVEIQWFTYNAGPSAMEAIFAGSLDVTYVGQGPAIN
                     AHLKSNGEEIRVISGAANAGAALIVKPDGGIKTAADFRGKKVATPQMGNTQDISCRAW
                     LKAQGFNVTQTGGDVLVVPTANPDQLALFQSGGVDAVWTVEPWVTRLEKEANGKVFME
                     DKDIITTWLVSSVKFLKDHRDLAKNIAAANKELTDWIQSHPAEAQQLLVGELKAETRT
                     EVSADTVTRSLKRIKLTTEVSPSFVKKAVLDGKNTGFLKGSTDTSKLIETL"
     gene            2246..3097
                     /locus_tag="VNW28_07970"
     CDS             2246..3097
                     /locus_tag="VNW28_07970"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA09905.1"
                     /translation="MYVTHVIPWHSRATSSAKEELEIIAPSKLSIENVSKSFKTATGT
                     VQALDRVSLKVGEGEFVCLVGASGCGKSTLLNIIAGLEKPDSGLVMADGKPVMSPGRE
                     RLVMFQEAALFPWLDVLGNVLFGLRLKPGLRDKDRLDVAKYYLELVGLTRFEHANIHE
                     LSGGMKQRVALARALAPNPRVLLMDEPFAALDALTREQLYGDIQRIWKARNKTIVFVT
                     HNVREAACLGDRVLLFSPHPGRVQEEFTIELPRPRDINSVDLAGFAGRIMRSLKGFTK
                     AEGEVAE"
     gene            3094..3864
                     /locus_tag="VNW28_07975"
     CDS             3094..3864
                     /locus_tag="VNW28_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09906.1"
                     /translation="MKRFASAAAFFGGLIILWQLLYDAHIWSPVLVPSPASVANYLRG
                     AAEDGTLGQATLITIRRLLIGYVIGIIFGLPLGLLTARWNVLRDTVGTLSLGLQTLPS
                     VCWVPLALLWFGQTEAAMLFVVVMGTLWSVVIATDTGVRNVPPIFRRAALTMGSKNMH
                     LWLRVILPAALPFVVSGMKQGWAFAWRSLMAAEIFVTILTGFGLGQLLHFGREQQAMD
                     QVIGIMVIIVVIGLLADKIFFSPIEQFLHRRWGTGASQ"
     gene            3874..4170
                     /locus_tag="VNW28_07980"
     CDS             3874..4170
                     /locus_tag="VNW28_07980"
                     /inference="COORDINATES: protein motif:HMM:TIGR01003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXA09907.1"
                     /translation="MVHSRREPVHASQQITIVNKLGLHARPAAQFVRRANAFRSEIWV
                     VTAAGRFSALSLIDVMRANLDCGMVATLEAHGTDAQEAVERLAKLVKELPDYDD"
     gene            complement(4167..5054)
                     /locus_tag="VNW28_07985"
     CDS             complement(4167..5054)
                     /locus_tag="VNW28_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase-like
                     domain-containing protein"
                     /protein_id="HXA09908.1"
                     /translation="MRPGKFLLLLCLLATAGLTTGASLPFSTVFQGREKFDRLVAQAQ
                     TNDWKSLPIGERTAAVGRAVVGTRYKSFTLEIDDHIEAPSANFNGMDCWTFFEIALGF
                     ARMLDEPQAQWTPATLLHYIELDRYRDGQCTGEYLSRLHYLEDWLADNDRRGLVKDLT
                     RDLGGVRVNHAAHEMTFGWRHYRYLRENPSLLAPLGRMEARVTARPLYQIPKNRVARI
                     ESGLKSGDIIGIVSRDRPGLYSTSHVGLACRTADGVLHFMHASSPQNYGRVVIDSRLS
                     SYLYRYGTDTGILVARPRR"
     gene            complement(5104..5610)
                     /locus_tag="VNW28_07990"
     CDS             complement(5104..5610)
                     /locus_tag="VNW28_07990"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXA09909.1"
                     /translation="MKRILVPVDFSPATDCVLALAHELARALQAELHLVHVREIAAVP
                     VFPAATIGYPGIGLPEMGMAGGLPVGSPGLGVADFPNEKEKSQLGALQDQLARSGLRA
                     FAHEPEGAVVEEILKVASEISADLMVMGSHGHGAVYNLLVGCVTEGVLKAGQCPVLLV
                     PAPPAAVK"
     gene            5708..6880
                     /locus_tag="VNW28_07995"
     CDS             5708..6880
                     /locus_tag="VNW28_07995"
                     /inference="COORDINATES: protein motif:HMM:NF017321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HXA09910.1"
                     /translation="MQDLFNEAEAETETKPDTAAPLASRMRPRSLDEFVGQEHILAPG
                     KLLRRAIEADRLPSVILSGPPGTGKTTLAQIIAAMTESKFERLSGVESNVAEMRRVVA
                     AATNRRRTSGKKTILFVDEIHHFNKVQQDILLPDVESGNLRLIGATTYNPFFYVNSAL
                     VSRSQVFQLESLGAEDLGKLIDRALQDNERGLGRYRVKMDPEARRHLAKLSDGDARKC
                     LNALEIGVLTTPPNEKGIIHFDLQVAEESIQTKAVVYDRAGDAHYDTISAFIKSMRAS
                     DEKGAIYWLAKMLHAGEDFRFIARRLVIFASEDIGLADAGALPLAIATQQAVEFVGMP
                     EARIPLAHATVYLCRAPKNREAYESLSAASEEIEAEKTRRVPEDLKNKHFPVNPEH"
     gene            6882..7598
                     /locus_tag="VNW28_08000"
     CDS             6882..7598
                     /locus_tag="VNW28_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09911.1"
                     /translation="MKLTHKISKLAQPLTYRRAARRLRRKLHPIRIGPLLARVDQDGL
                     RALQARHDSPPPDAPSSWHHYSKYLDLKKYLPLNIRRVQDLNLHRTPPREILDIGCGG
                     GFFLFVAQAQGHRGLGLDTPGIPVFDDLVELLGVERIIYTIKALEPLPALGRKFDLIT
                     AYSTAFHGGRDQSWRWGAKEWEFLVADLEQHLNPGGQIFFGLNPAYGGDYYTPEILEM
                     FLRRGAAVERENVLFPPKRS"
     gene            complement(7607..7879)
                     /locus_tag="VNW28_08005"
     CDS             complement(7607..7879)
                     /locus_tag="VNW28_08005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXA09912.1"
                     /translation="MKAHLIKAACEVITDQKMLVNVISRRVRQLAFGHRPLVACPPGM
                     GLADVALTEVIEGKLTYESTLGAKETDAVKTVVPFPAKKATQKKAA"
     gene            complement(8242..8616)
                     /locus_tag="VNW28_08010"
     CDS             complement(8242..8616)
                     /locus_tag="VNW28_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09913.1"
                     /translation="MISIFTSWLWMGVIFHRFQKRTPQTWRAEGNGSYAMSAAIHFAA
                     CIALATVFLFVARMPGNLFAGGIHGALRFAALTWMAVAAPFAIEAAIFINLHPWVVVG
                     QVLDWLTTSVLACAIAAWWTRT"
     gene            8994..9737
                     /locus_tag="VNW28_08015"
     CDS             8994..9737
                     /locus_tag="VNW28_08015"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXA09914.1"
                     /translation="MAFKDHFSRQATDYAKFRPQYPRALFQFIAALAPNDRVALDGAT
                     GNGQAAVALAEFFQKVIALDASPDQIARAQPNERVEYRVAAAESTGLAAHSCAALTVA
                     QALHWFERDAFYAEARRILLPGGVLAVWAYNELRITPGVDALVRRFHDDIVGPYWPVE
                     RKIVGRGYRNLPFPFAEIEPPELEIEVRWSLRHLLGYLRTWSATQRFLAAEGRDPVDE
                     VARDLEAAWGDAATERPAIWPLTIRIGRA"
BASE COUNT         2111 a         2990 c         2968 g         1948 t
ORIGIN      
        1 tacatccagg caaacttcga agatggcgat gtctcggcct tcgaggggcg attcggtttg
       61 agtgcgctga aagaccgttt tcgtctgcgc cgcgcgcgca tcaacctgag cggtgatttt
      121 gccgagcagt tcgacttcaa ggtcgaaggc gactttgagc agagcgactc tgcgatcacg
      181 gccctgaagt cggtcagtac gacaaccggc aagacgacca cggtgagcaa tgcgacgcgg
      241 acggagtttt ccgcgaccga cgtcttcatt aactggcacg ggattccgga ggcgaacatc
      301 aaggtggggc aatggaaagc cccctttggt ctggaacaac tgacgcccga tcccaagctc
      361 ttcatcatcg agcgcagtct gcccaccggt gccctcacgc cggagcgcca ggtcggagtg
      421 caagtttggg gcaagccact gaccaatctg tggccgcagg agacggacct ggtgacctac
      481 tacgctggcg tctttaacgg caacggccgc aacgtcaaca ccaatgataa caacaatttc
      541 atgtatgtgg gtcggctgga attgctgccc ttcaagggca aggtggcggg ccaggaggtg
      601 agcttgaagc tgggcggcga tgtcctcaac agccgcgacg atgccggaac gatcatctcc
      661 ccctcgggta acttgaaggt caacactgac ggctcactcg cagcgtacac gctgccgggc
      721 gcggccgaaa gaacggcctg gagcgtggac gcgtggttaa acatcggtcc ctttgatctg
      781 atcggcgagt atctctccga agatgtcaac ggccggacgg tgaacggggt ggcccccggc
      841 tttaccgact tcagtccgag tggctggtac gtgcaaggca gttacttcat tatcccgaaa
      901 aagttccagg cggcggtccg ctgggaagcg cttaaccctg ggcaattggg ccaagacggg
      961 atccactcca ttaccggcgg cctcaactac tacatccacg gggatgctct caaagtgatg
     1021 gcgaactacg tgcacacctg gtccgatttt cgcgccgcca acccaacctt tggcgatgac
     1081 cagttcgacg aagtcatcct gcgcctccag ttgatgttct agtttaagag acccaagaac
     1141 tttctgtcgc gctcgtgtct cagcgatcgc gctccaaatg accatgccca gcttcgggtt
     1201 ttttgtaacc acattcagct tttgaagatc aaacctatga aactacacac actcgcagca
     1261 ctcatcggcg cagcaatcct ttgcgcgacg agtctaaacg ccgaagagaa aaccaccatc
     1321 cgcttcgggc atttcccgaa tgttacccac gcccaaggcg tcattgctca cgcactctcg
     1381 cgtcaaggca aagggtggtt cgaacaacgg ctcgggccga acgtcgaaat tcagtggttc
     1441 acttacaacg ccggaccttc cgcgatggaa gcgatcttcg ctggatcact cgacgtcact
     1501 tacgtcggcc agggaccagc catcaatgcg catttgaaat caaatggcga agagatccgt
     1561 gtcatctcag gcgcggcaaa tgcaggagcg gctctcatcg tgaaacccga cggcggcatc
     1621 aaaacggcag cggatttccg gggcaagaag gtcgcgacgc cgcaaatggg caacacgcag
     1681 gatatctcat gccgggcatg gctaaaggcc cagggattca acgtcacgca aaccgggggc
     1741 gacgtgttgg ttgttccaac cgccaaccct gatcaactgg ccttgttcca gagcggcggg
     1801 gttgatgcgg tctggaccgt ggagccctgg gtaacacgac tcgaaaagga agccaatggc
     1861 aaggtcttca tggaagacaa ggacatcatt accacctggc tcgtttcaag tgtgaaattt
     1921 ctgaaggatc accgcgatct cgcgaaaaac atcgcggcgg cgaacaagga gcttaccgac
     1981 tggatccaat ctcatcccgc tgaggcacaa cagctactgg tgggcgagtt gaaagctgag
     2041 acaagaacag aggtttccgc ggataccgtc acgcgttctt tgaagcgaat caaactcact
     2101 accgaggtct caccctcatt cgttaaaaag gcggtgctgg atggaaaaaa tacgggcttc
     2161 ctgaaagggt ccaccgacac ttcgaagctc atcgaaacac tctaagcaga ttgcccattc
     2221 gaaaaggcac aaactcctct tcgccatgta tgtcacccac gtaataccct ggcattccag
     2281 agccacctct tcagccaaag aagagttaga aatcatcgca ccgagtaagc tttcgatcga
     2341 aaacgtctcg aagagtttca agaccgccac tgggacggtg caggcgctcg atcgcgtgag
     2401 cttgaaagtg ggcgaaggcg aattcgtctg tctcgtcggc gcgagtggtt gtgggaaatc
     2461 cactttgctc aatatcatcg ccgggttgga gaaacccgac agcggtttgg tgatggcgga
     2521 cgggaaacca gttatgtcgc ccgggaggga gcgtctcgtg atgttccagg aggcggccct
     2581 ctttccatgg ctcgacgtct tgggaaatgt gctcttcggg ctgagattaa aaccagggct
     2641 acgggacaaa gaccgccttg atgtagcgaa atactatctc gaacttgttg gactaactcg
     2701 cttcgaacat gccaacatcc atgagttatc gggcggaatg aaacagcgcg tcgctttggc
     2761 ccgagcgctt gctccgaacc cacgggtgtt gttgatggat gaaccgtttg cggcgcttga
     2821 cgcgctgacc cgagagcaac tttacggcga catccagcga atctggaagg cgcgaaacaa
     2881 aacgatcgtt tttgttacgc ataacgtccg cgaagccgcg tgcctgggcg atcgagtgct
     2941 tctcttttcg ccccatccgg gacgcgtcca ggaagagttc acgatcgagt tgccgcgtcc
     3001 gcgcgacatc aacagcgttg atctcgctgg tttcgccggt cgaatcatgc gctccctaaa
     3061 gggctttact aaagcggaag gagaagtagc ggaatgaaac gatttgcgag tgcggccgcc
     3121 tttttcggtg gcttgatcat cctttggcag ttgctctatg acgcgcacat ttggtcacct
     3181 gtgctcgtgc cttctcctgc ttccgttgcg aattacttgc gcggtgcggc tgaagacgga
     3241 acgcttggac aggccaccct tatcaccatc cgccgtttgc tgatcggcta cgtcataggt
     3301 ataatttttg gtttaccctt ggggttgtta acggcgcgct ggaatgtatt gcgcgacacc
     3361 gttgggacac tctcgctcgg cttacaaacg ttgccaagtg tttgctgggt gccgctggcg
     3421 cttctctggt tcggacaaac tgaggccgcg atgttgtttg tcgtggtcat gggtaccttg
     3481 tggtcggtcg tcattgctac tgataccggc gtgcgcaacg ttccaccaat tttccgccgt
     3541 gcggccctca ccatgggttc aaaaaacatg cacctgtggt tgagagttat tctgcctgcg
     3601 gcgctgccgt ttgtggtgag cgggatgaag caagggtggg ccttcgcctg gcgatccttg
     3661 atggccgcag aaattttcgt caccattctg accgggtttg gtcttggtca gctcctgcac
     3721 ttcggacgcg agcagcaagc catggatcag gtcatcggca tcatggtgat aatcgttgtc
     3781 atcggcttgc tcgcagataa aattttcttt tcgccgatcg agcagttcct tcatcgacgc
     3841 tggggcaccg gcgccagcca ataaccgatg ttaatggtcc actcccggcg tgaacccgtg
     3901 cacgcctctc aacaaataac gatcgtcaac aagcttggcc tgcacgcccg gccggcggcc
     3961 caattcgttc gccgcgctaa cgcgttccgc tctgaaatct gggtcgtgac ggcggccggc
     4021 cgattttcag cgttgagtct gatcgatgtg atgcgcgcca acctcgactg tggcatggtc
     4081 gccaccctgg aggcgcacgg caccgacgcc caggaagccg tggagcgact tgccaagctc
     4141 gtcaaagaac tccctgatta cgacgattag cgccgcggtc gcgcaacgag aattccggta
     4201 tcggtaccat agcggtagag ataactcgaa agccgcgaat cgatcacgac tcgcccatag
     4261 ttttgcggtg atgaggcgtg catgaaatgc aggacgccat ccgccgttcg gcaggccaat
     4321 ccgacgtgcg aggtcgaata aagccccggc cgatcgcgcg agacgatgcc tatgatatcg
     4381 ccgctcttca gcccggactc gatccgggcc acgcggtttt tcggaatctg atagagcgga
     4441 cgcgccgtca ctctggcttc catccggccg agcggcgcga gcagagaggg attctctctc
     4501 aggtaacgat agtgccgcca gccaaaggtc atctcatgcg cggcgtgatt gacgcgcacg
     4561 ccgccaagat cccgggtcag atccttcacc aggccgcgcc gatcgttatc cgccagccag
     4621 tcctcgagat aatgcagccg ggaaaggtat tcacccgtgc actggccgtc gcgataacga
     4681 tcgagctcga tataatggag cagcgtggcg ggagtccact gcgcctgcgg ctcatcgagc
     4741 atgcgggcga agccgagcgc gatctcgaag aacgtccagc aatccatccc attgaaattg
     4801 gcggagggcg cctcgatgtg gtcgtcgatc tcgagggtga aggatttgta gcgcgttccg
     4861 accacggcgc ggccgaccgc ggcggttcgc tccccgatag ggagcgattt ccaatcgttt
     4921 gtctgggcct gcgccaccag ccggtcaaac ttttcgcgac cctggaagac cgtggaaaag
     4981 gggagcgagg ctccagtcgt taggccggcg gtcgctaaga gacaaagaag aagcaggaat
     5041 tttccgggac gcatctgctg aaaccctgcg gacgaaagcc gtccggcgca aggcggtcgg
     5101 cgcttatttc actgcggcag gcggagcggg cacgagcagg accgggcact gccccgcctt
     5161 taaaacacct tcggtcacgc agccgacgag caggttgtaa accgcaccgt ggccatgcga
     5221 acccatcacc atcagatcgg ccgaaatctc gctcgccacc ttgaggattt cctccaccac
     5281 cgctccctcc ggctcatggg cgaacgcgcg cagtccgctc cgcgccaact gatcctgcag
     5341 cgcgccgagc tggctctttt ccttttcgtt agggaaatcg gcgactccca ggccggggga
     5401 cccgactggc aggccgcccg ccatccccat ttccggcaag ccgattcccg gatagccaat
     5461 cgtggcggcg ggaaaaacgg gcaccgcggc gatttcgcgc acgtgcacaa ggtggagctc
     5521 ggcctgcagc gcgcgcgcca gctcgtgcgc cagcgccagg acgcaatccg tcgcggggga
     5581 aaaatccact gggacgagga ttcgtttcat gctcggggag ccgcttcgcc ggctcgcgaa
     5641 aggatgcggg aacgtgttgc cgtggtcaag cggctgacaa acgagctgtg cacccttatt
     5701 ctccggcgtg caggacctct tcaacgaggc ggaagctgaa acggaaacga aacccgacac
     5761 cgctgcgccg ctcgccagcc ggatgcgccc gcgctcgctc gacgagttcg tcgggcagga
     5821 acacatcctt gcccccggca aactgctccg gcgcgcgatc gaggccgatc ggctcccgtc
     5881 ggtcatcctc tccggcccac ccggcacggg caaaacgacg ctggcccaaa tcatcgcggc
     5941 catgacggaa tcgaaatttg aacgcctgag cggggtggag agcaatgtcg cggagatgcg
     6001 ccgggtcgta gccgccgcga ccaaccgccg ccgcacctcg ggaaagaaga cgattctgtt
     6061 tgtggacgag attcatcatt tcaacaaagt gcagcaggac attttgttgc ccgacgtcga
     6121 gagcggaaac ctgcggctga tcggcgcgac gacttacaat ccgttttttt acgtcaacag
     6181 cgcgctcgtc tcgcgctcgc aggttttcca gttggagtcg ttaggcgcgg aggatctcgg
     6241 gaagttgatc gatcgcgcgc tccaggataa cgaacgcgga ctgggccggt atcgcgtgaa
     6301 gatggatccc gaggccagac gccacctcgc gaagctgagc gatggcgacg cgcggaaatg
     6361 cctcaacgcc ctcgagattg gcgtgctcac gaccccgcct aacgaaaaag gcattattca
     6421 tttcgacctc caggtggccg aggagagcat ccagaccaaa gccgtcgtct acgatcgcgc
     6481 gggcgacgcg cattacgata cgatcagtgc ttttatcaag agcatgcgcg cttccgacga
     6541 gaagggcgcg atctactggc tcgccaaaat gcttcacgcc ggggaggact ttcgttttat
     6601 tgcacggcgc ctcgtcattt tcgcgagcga agacatcgga ctggccgatg ccggggcctt
     6661 gccgctcgcc atcgcgacgc aacaggcggt cgaatttgtc gggatgccgg aggcgcgcat
     6721 tcccctcgcc cacgctacgg tctacctctg tcgcgcgccg aaaaaccgtg aagcctacga
     6781 atcgttaagc gcggcgagcg aagaaatcga ggcggaaaaa accaggcgcg tccccgaaga
     6841 tttgaagaac aaacactttc ccgtaaatcc ggagcactag cgtgaaactc acgcacaaga
     6901 tcagcaaact cgcccagccc ctgacctatc ggagggctgc gcgacggctg cgccggaagc
     6961 ttcatccgat tcggattggg ccccttctgg cgcgggtcga ccaggacggc ttgcgcgcac
     7021 tgcaggccag gcacgattcg ccgccgcccg acgccccgag cagctggcat cattactcga
     7081 aatacctcga tctgaaaaaa tatctcccgc tcaatatccg gcgcgtgcag gacctgaatc
     7141 tgcaccgcac cccgccgcgc gaaatcctcg atatcggttg tggtggagga tttttcctct
     7201 ttgtcgccca ggcgcagggt catcgcggcc tcggtctaga cacacccggc attccggtct
     7261 tcgacgacct ggtcgagcta ctcggcgtcg aacgcattat ctacacgatt aaggcgctcg
     7321 aaccgttgcc ggcgctcggc cgcaaattcg atttgatcac cgcatactct accgcgtttc
     7381 acggtggccg ggaccagtca tggcgctggg gcgcgaagga atgggaattt ctcgtcgccg
     7441 acctggaaca gcatctgaat ccgggtggcc agattttctt cggactcaat ccggcctacg
     7501 gaggcgatta ttataccccg gagattctcg agatgtttct ccgtcgcggg gcggcggtgg
     7561 agcgggagaa cgttcttttc ccgccgaagc ggagctagag gtcgagctac gccgcctttt
     7621 tttgcgtggc ctttttcgcc gggaaaggga ccacggtttt gactgcgtcg gtttcttttg
     7681 ctccgagcgt ggactcgtag gtcagtttcc cttcgatcac ttcggtcagc gccacatcgg
     7741 ccaaacccat tccgggagga caggcgacca gcggacgatg accgaaggcg agttggcgga
     7801 cgcgccggga gatgacgttg accagcattt tctggtcagt gattacttcg caggcagctt
     7861 tgatgagatg agctttcata agataacgaa ccgagcgtcc gcggctaacg acgagttatt
     7921 ccccgcacag ggattggaaa ccgggaggtg ggttccgatg aaaatcgcag cagagaaact
     7981 tccgcattat cgtctttccg cgccaaaaca caacgggaat gtccgaggcg gctctgcgcc
     8041 ctacaaccgc gagagcatcg ccgcgagcag atcgacggca aagcaaacca gacaaggatc
     8101 acctgcgaga agcgcaaatt gcgcgccgcc cagtaatcgg ccgctacctg cggatcgcgc
     8161 tcgaggagcg gcgaccggga agtccgactt ctgtcgccag gagtaaatcc gaggtcttgt
     8221 tagtgacctg ccagcgcggc actaagtccg cgtccaccag gccgcgatcg cgcaggcgag
     8281 cacggaggtc gtgagccaat caagcacctg gccgaccacc acccagggat ggagattgat
     8341 aaagatcgcg gcttcgatcg cgaacggagc cgccaccgcc atccaggtaa gcgccgcgaa
     8401 gcgcagcgcg ccgtggatcc cgcccgcaaa caagttgcct ggcatccgtg cgacgaatag
     8461 aaagacggtc gcgagcgcga tgcaggccgc gaaatggatc gcggcggaca tcgcgtagct
     8521 gccatttccc tcggcccgcc aggtctgcgg agtgcgcttt tggaagcgat ggaaaatcac
     8581 tcccatccaa agccaactgg tgaaaattga aatcaccccg gcaattgcac cgggaacgag
     8641 agccagctgc agtgttctca tatcgtcgga agctgaccca gctttgacgc gccaagcgcg
     8701 attgtcacgc ccgaaatggc ctcgcgcggc gcgcggtagt gttcgtcgat tgtccgttgc
     8761 gggttgccgc cagtcgtagc cgggatggat gacccccggg gcgcaccgaa ctgtggggaa
     8821 cgctcgcccc ccgggatcac cgtcttgcct accaactcac gcccgcgttc agagcgaatc
     8881 gaccaattga caatcaagga accccctttc cagtctcgcg cgctggccgg ataacaactc
     8941 gcggtgaaag cctttgagtc ccttccgtct cgtgcgagat gaagccagga ggcatggcgt
     9001 tcaaagacca tttttcacga caggcaacgg actacgcgaa gtttcgcccg caatatccgc
     9061 gggcgctctt ccagttcatc gccgcgcttg cgcctaacga ccgggtggcg ctcgatggcg
     9121 cgacgggcaa cgggcaagcc gcggtcgcgc tggccgagtt ttttcaaaaa gtgatcgcgc
     9181 tcgacgcgag cccagaccaa atcgcgcggg ctcagcctaa cgaacgagtg gaatatcgcg
     9241 tcgccgcggc cgagtcgacc ggtttggccg cccattcctg cgccgcgctg accgtagcgc
     9301 aggcgctgca ctggttcgag cgggacgcat tttatgcgga agcccggcgc atcctgctac
     9361 cgggaggagt actggcggtc tgggcctata acgagttgcg aatcaccccg ggagttgatg
     9421 cgctcgtgcg acgttttcac gacgacatcg tcggacccta ctggccggtg gaacgaaaga
     9481 tcgtcgggcg cgggtatcga aatctgccgt tccccttcgc ggaaatcgag ccaccggagt
     9541 tggaaatcga ggtgcgatgg tcgttgcgcc acttgctcgg ctaccttcgg acgtggtcgg
     9601 cgacacagcg tttccttgcg gcggaaggcc gcgatccggt ggacgaggtc gcacgcgatt
     9661 tggaggctgc ctggggggat gcggcgacgg agcggcccgc tatttggccg ctcacgattc
     9721 ggatcgggcg agcttgagct acccttgccc atcatcccgc tcctgatcct tctccgtcaa
     9781 aggcggttgg agcacgatta cgagcaggag caagccggga aaatgaagag ggcgggtctt
     9841 gcgacccgcc ctccctttcc gttgagcagc ttctccctag cttttgctct gcgaaactac
     9901 ttcgttcagc ttgaagatcg gcaggaacat cgcaatcacg atgccaccca cgatcacccc
     9961 gaggaacacg atcaggagcg gctcgatcaa cgaggtcatc gcatcaagca tcgcttc
//
LOCUS       DATMNS010000228        12399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4986830, whole genome shotgun sequence.
ACCESSION   DATMNS010000228 DATMNS010000000
VERSION     DATMNS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 12399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12399
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4986830"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VNW28_08020"
     CDS             <1..692
                     /locus_tag="VNW28_08020"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HXA09915.1"
                     /translation="ERFRALALAAIQDINRRGKLALVVGGSGLYLKALTHGLAPLPPG
                     DSKLRAELNLRSLDELNARLAAVDPLGAAKVDRRNKRRVVRALEIFAQTKTLASAQRS
                     QWAGESPGAPGVFIFRERSELYARIDRRVEEMFREGVAEEVGRIGSLSATAAKTLGLD
                     QVRQLLAGQISATQCRAAIQQATRRYAKRQLTWFRRQPNLEPLNLSLLKDHAAAVDWI
                     LQRVSLLPPLQ"
     gene            689..1987
                     /gene="hflX"
                     /locus_tag="VNW28_08025"
     CDS             689..1987
                     /gene="hflX"
                     /locus_tag="VNW28_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXA09916.1"
                     /translation="MIETQPHRKQERALLIGLEKEGVSKWDLRDSLDELRELASSAGA
                     QVVDTVTQKLQKPTAPFYIGKGKAELIKESFQDQEVTSVIFDNELSPAQGRNLENLFS
                     RKVLDRTQLILDIFAQRARSREGRLQIELAQLQYLLPRLTRMWTHLSRQSGGIGTRGP
                     GETQLEVDRRRVQERIARLERDLNEVRKHRAVQRQGRKRHQWPVAAVVGYTNAGKSTL
                     LNLLTGADLLAEDKLFATLDPTTRSLILPNKQRVLLTDTVGFLRNLPHTLIESFKATL
                     EEVVEADLLIHVVDLSHPRLDEHMAAVDAVIKEIGAYGKQTLIIFNKIDALADKDLAQ
                     FYLEKFPGSVAISARTGEGVDGLVPALQNVLASWRLRCRYRLPQRESALLAEVHRVGH
                     VLELRYEGEFVELVAHVPPQLEQRLSPFLDHSPAAAPSAT"
     gene            1994..2287
                     /locus_tag="VNW28_08030"
     CDS             1994..2287
                     /locus_tag="VNW28_08030"
                     /inference="COORDINATES: protein motif:HMM:NF021193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HXA09917.1"
                     /translation="MKWYWKWVHRLGLANRPRVRKLIVAVIGSTVLLFGLALIVLPGP
                     AVLVVPLGLALLATEFAWARRLIRLGGVFSGRAWRQWWRKGPASEESLGHDES"
     gene            2274..2939
                     /gene="nth"
                     /locus_tag="VNW28_08035"
     CDS             2274..2939
                     /gene="nth"
                     /locus_tag="VNW28_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXA09918.1"
                     /translation="MTRAERAERLVKMLPKVYPDAHCELDFRTPLQLLIATILSAQCT
                     DKRVNLVTPALFARYRTAADYAAAPPAELEKAIQSTGFFRNKTKSIRAATAAIVREHG
                     GRVPQTMAELHALPGVGRKTANVVLGNAFHKDEGVVVDTHVARLSERLGLTNQKEPEK
                     IERDLMRLIPKKHWTNWSHWLIWHGRRRCFARQPDCAHCELFQLCPAGKKFIRAGTAR
                     ASV"
     gene            complement(2931..3083)
                     /locus_tag="VNW28_08040"
     CDS             complement(2931..3083)
                     /locus_tag="VNW28_08040"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09919.1"
                     /translation="MTGARNRLMDLETLSDHDLEEFQKEFERLRKRSKATAAAVSKMS
                     RKTGSD"
     gene            3164..4060
                     /locus_tag="VNW28_08045"
     CDS             3164..4060
                     /locus_tag="VNW28_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09920.1"
                     /translation="MYTMSDAVLHLNLDDAWHLPTLALPSADARDWGPRLRFSARPRE
                     IEQFQREWVQRLPPFLVYGSGDFHHLTALWLRRLTRLTTVVSFDNHPDWDIRPPRWCC
                     GGWVNRALELPQVEKVSVWGCGNFECWWPGRIFGNNRAERAGSLEVHPWADDRSAKER
                     QRRGAILRHNWREKFARFVEQLGAAQVYVTVDLDCLRAEDAVANWESGRFRTEDVAWA
                     IESLGKGGRIVAGDICGAWSPALYARRKQRFASEMDHPKLPPRDPAEARRTNEAALRR
                     LLPALTERNEDHSQGDDQRADR"
     gene            complement(4004..4348)
                     /locus_tag="VNW28_08050"
     CDS             complement(4004..4348)
                     /locus_tag="VNW28_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXA09921.1"
                     /translation="MTPFAALLIFVTVGCFVSGQILLKHAMDITARANYSRGHATLVF
                     AAAIAVFAVNFFVNIGLLQRFDLSYFFPFQGLSVILIALAGAFVLKEKLTLRLTIGSL
                     IITLGVVLVSLS"
     gene            complement(4345..4776)
                     /locus_tag="VNW28_08055"
     CDS             complement(4345..4776)
                     /locus_tag="VNW28_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09922.1"
                     /translation="MRSTAEPAGPGLIRRWFLNPWIQLSICVLLGTAAELFLKLGAVE
                     TANPANAWSWTGLTGLRSAWVWWGIIASVVSLFNWLATLRKLPLTIAFPVGNVVHIFV
                     PLGSWIFLGEAIVTRRWFGIALLVLGLMIMAKPAAKLEERL"
     gene            complement(4815..5915)
                     /locus_tag="VNW28_08060"
     CDS             complement(4815..5915)
                     /locus_tag="VNW28_08060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXA09923.1"
                     /translation="MQDCPAWREAFLDQCKDHRYYELIGTTLTDNFDYFFLVLEDNAG
                     RVRAIQPLFFVRQNLTEGVAGPIRATVDRIRRKFPRFLTMKALMVGSTAGEGHLGACR
                     PEDAEWVAEALHSCLKVFAREARASLVVLKDFPARYRAPLRNFSANGYTRIASMPMTR
                     LSLHYRNFDEYVASLGKATRKNLRRKFRKTAQSAPIQCEVLTDVTPVIDELYPLYLQV
                     HERSKLRFERLTPEYFCRLGREMPERTRFFVWRQEGKAIAFSLCLVHDGTIYDEYLGL
                     DYRVAFDLHLYFYTMRDIITWALAQGLHSYCSSPLNYDPKLHLGCELVPLDLYVLHTG
                     ALLNPIFRHALRFLQPTKHDPVLQQFANAHEL"
     gene            complement(6085..7206)
                     /locus_tag="VNW28_08065"
     CDS             complement(6085..7206)
                     /locus_tag="VNW28_08065"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HXA09924.1"
                     /translation="MNLLWLSTPLFDLGRYHISLLGIVAFVGLMALGLISAKALQSAV
                     VRRFLSRLKLEANFVAIITTILSLAALVFFFVGAINGLGIPLAWSDPLPGIPISLLQL
                     FLLVGLLILVFWLSARTKRFLFNRFLVHSGLDRSLQYAISQIASNVVLVVGIFIVLQN
                     TGIHLEALTVFAGAIGVGIGFGLQNITSNFISGLVILAERPIAIGDRVEVAGVTGQVQ
                     KIRARCTVVVTNDNITTIVPNQKFIDSPVTNWTYGDPRVRFRIPVGVAYGSDVEKVRR
                     TLIEAGQEDPHVLENPAPSVFFVGFGDSSLKFELVVWSNEMSNRPNRFRSDLNFAIHR
                     KLGAAGIEIPVPQRDLHVKSGLLKVESAPGKPAPITRTE"
     gene            complement(7319..7567)
                     /locus_tag="VNW28_08070"
     CDS             complement(7319..7567)
                     /locus_tag="VNW28_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXA09925.1"
                     /translation="MSDKPVEEKVKDIIVEQLGVNPEQVTPNASFIEDLGADSLDIVE
                     LVMAFEEEFSVEVPDEDAEKLQTVGDVVKYIEDKAAKE"
     gene            7742..9331
                     /locus_tag="VNW28_08075"
     CDS             7742..9331
                     /locus_tag="VNW28_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09926.1"
                     /translation="MPEENDAPDLLDLKFLPAWVKETPNENLYAHFAGEEAAAVGQRD
                     RRGPDRRGGERGERGPRPTREKGSRRDPRERARGPADQRPREPRRPEEPAPPAPAVEV
                     RFVPDARVLENVLAQIKAGHLAYSVFSLARMFLEKPERYDVRLKAETAALFQLGENGQ
                     VASDQRVLENGAFLNEKDHFYRTEVTQSDPIKGNFTTVARCRLSGVLLGPTNHHAYQP
                     QLRNLYEQRFSRRMSFQDYQRQVEIVSNPEAVERWKEEARQVTTYVTLQEEPPITFQN
                     PAETERHFRQTYLPGLLRQSLELTLDGVVSRRLADRSLGRVIEDGWAQEARSPTKIMQ
                     ELIGAFRQGGLHIFRHRKAMLFVSPIRARPFHHESSSVSSTVAAILAALSANPGINRK
                     QLLERLPPDTAGEPESVEKKKLALASDLHWLINEGHVIEFNDGSLDLTRTKAPPANAR
                     AQARSAAPNGIAEGLASPNDSKNTSETSAAPAVEEHPAEIAREGDRVPEGEALPPAVA
                     EITPDGAPEGSSPAKNALGAG"
     gene            complement(9328..9597)
                     /gene="rpmB"
                     /locus_tag="VNW28_08080"
     CDS             complement(9328..9597)
                     /gene="rpmB"
                     /locus_tag="VNW28_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXA09927.1"
                     /translation="MSKVCSITGSKPSRGSVIHRRGMAKKKGGVGRHVTKNVPRTFQP
                     NLREQRIWVAELKKFVRVKTTARGLKTINKNGAYATLKKAGLIAA"
     gene            9964..11010
                     /locus_tag="VNW28_08085"
     CDS             9964..11010
                     /locus_tag="VNW28_08085"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXA09928.1"
                     /translation="MSHKTNHKLRLGIVGAGEVVRRRHWPALQRQSDIELVAVCNSSY
                     ESAEKFCRENAPHATPMQNWAELLGMADLDIVWIGTPPYMHSAVTVSALEAGKHVFCQ
                     ARMAMDLAEAEEMLSVSRRFPELVTMLCPPPHGMRGDLLVQKLLAEKTLGRPYHLRLQ
                     SLDSAFLDPDAPAHWRQRVEMSGLNTLTLGIYVEVLQHWFGQITGVFARGRILQPMRQ
                     GYEVIIPDLLTVLCTFANGMEGVLEFSGVDAHAPADRLEVYGDLGTLTYDFGADAVHA
                     GQIGDRALHVVELTPDLETNWHVEDDFIAAVKSRGRINPHPDFEDGVRYMRVVQAVAD
                     SRARGEWVAINDRA"
     gene            complement(11026..>12399)
                     /gene="mgtE"
                     /locus_tag="VNW28_08090"
     CDS             complement(11026..>12399)
                     /gene="mgtE"
                     /locus_tag="VNW28_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXA09929.1"
                     /translation="QSLIDARNFSALRELFSEMPPADVAEIILDLPEDEQVIIFRILP
                     HALAADVFEYLAVDVEAQQKLLRGMAHEQVVSILNEMSPDDRTALLEELPSAAARQLI
                     RLLTPEERRIAQALLGYPEGSVGRLMTPDLVAADANWTVKEVLDYIREHGQDSETLNV
                     VYVVDDRGKLVDDIRIREFLLKPLAAKVSDLMDENFIALNVTDSQEEAVNVFRKYDRT
                     ALPVVDSTGVLVGVVTIDDMLDVAEEEATEDIQKFGGMEALDEPYMRIPLWRMVQKRA
                     GWLVILFLGEMLTATAMANYEDEIAKAVVLALFLPLIISSGGNSGSQASTLIIRAMAL
                     GEVTLRDWWRVASRELQTGLSLGLILGVIGTVRVAVWSIVGEQYFHRSLYGPHWPLVA
                     LTVGAALVGVVLWGSLAGSMLPFLLRRLGADPATSSAPFVATLVDVTGLIIYFSIALL
                     IMRGAML"
BASE COUNT         2465 a         3729 c         3901 g         2304 t
ORIGIN      
        1 cggagagatt tcgcgctctg gcgttggccg caattcagga catcaaccgt cgcgggaaac
       61 tggccctcgt ggttggcggg agcgggctct acctcaaggc cctgacccac ggcctcgcgc
      121 cgctgccgcc gggggattcg aagttgcgcg cggaacttaa cttgcggtcg ctcgacgaac
      181 tgaatgcgcg tctcgccgcc gtcgatccgt taggcgcggc gaaggtcgat cgacggaaca
      241 aacggcgggt tgttagggcg ctggagattt tcgcgcagac aaagacgctg gcttccgcac
      301 agcggtcaca atgggcgggc gaatcacctg gtgcgcccgg cgttttcatc ttccgggagc
      361 gctctgaact ttacgcgcgc atcgatcgcc gggtggaaga gatgtttcgc gaaggggtgg
      421 cggaagaggt cggccgcatc ggttcactga gcgcgaccgc cgcgaaaacc ctcgggctgg
      481 atcaggttcg gcaacttctt gcgggtcaaa tcagcgcgac ccagtgtcgc gcagcgatcc
      541 agcaggcgac ccgccgttat gccaaaagac agttgacctg gtttcggcgg caacctaatc
      601 ttgaaccact gaatttgtca ttgttaaagg atcacgcagc cgccgtcgat tggatcctgc
      661 aaagggtttc ccttttgcct ccccttcaat gattgagacg cagccgcatc ggaaacagga
      721 gcgcgcgctc ctcatcggac tcgaaaaaga aggtgtttcg aaatgggatt tgcgcgattc
      781 gctcgacgag ctgcgggagc tggcgagctc ggcgggcgcg caggtggtcg acaccgtgac
      841 ccaaaaattg cagaagccga ccgcgccgtt ctacattggc aaagggaaag ccgagttgat
      901 caaggagtcg ttccaggatc aggaagtgac ctcggtcatc ttcgacaacg agctctcacc
      961 ggcgcagggc cggaacctgg aaaatctctt ctcgagaaaa gtgctcgatc gcacccagct
     1021 gatcctcgat atcttcgcgc agcgcgcccg gagccgcgaa ggccggctgc aaatcgagct
     1081 cgcgcaactg caatatctcc tcccgcgcct aacaagaatg tggactcacc tttcccggca
     1141 gagcggcggc atcggcacgc ggggcccggg cgaaacccag ctcgaagtcg atcgccggcg
     1201 ggtgcaggag cggatcgcgc ggctggagcg cgatctcaac gaagtgcgca agcatcgcgc
     1261 ggtgcagcgc cagggccgga agcgtcacca gtggccggtg gctgcggtcg tcgggtatac
     1321 caacgcgggc aagtcgaccc tccttaatct cctcaccggc gccgatctgc tggcggagga
     1381 caaacttttc gcgaccctcg atccgacgac gcggagcttg attttgccta acaaacagcg
     1441 cgtcctgctg accgacacgg tcggcttcct gcgcaatctt ccgcacactt tgatcgagtc
     1501 gttcaaggcc acgctcgagg aggtggtcga ggccgacctg ctcatccacg tcgtcgatct
     1561 gagtcatccg cgtctcgacg aacacatggc cgcggtcgat gccgtgatca aggagatcgg
     1621 cgcttacggg aagcaaacgc tgattatttt caacaagatc gatgcgctgg ccgacaagga
     1681 tttggcgcag ttttatctcg agaagtttcc cggcagcgtc gccatctccg cgcgcaccgg
     1741 tgaaggcgtc gacggactgg tcccggcttt gcagaatgtg ctcgcctcct ggcggctgcg
     1801 ctgccgttat cggctcccgc agagggaatc ggccctgctc gcggaagtgc atcgggtcgg
     1861 tcatgtcctt gagttgcggt atgaaggcga gtttgttgag ttggtcgcgc acgttcctcc
     1921 ccaattggag caaaggctct caccgttcct tgaccattcg ccggcggcgg ccccctccgc
     1981 gacctaacga gcggtgaagt ggtattggaa atgggtgcat cgcctcggcc tcgccaatcg
     2041 gccgcgcgtc cgcaaactga tcgtcgcggt catcggctcg acggtgctct tgtttggtct
     2101 ggccttgatc gtcctgccgg gccccgcggt cctggtcgtg ccgcttggtc tggcgttgct
     2161 ggccacggaa tttgcctggg cgcgccggct catccggctg ggcggtgttt tttcgggcag
     2221 agcgtggcgg cagtggtggc gcaaggggcc cgcttccgag gaatcgttag gtcatgacga
     2281 gagctgagcg agcggagcga ctggtcaaaa tgctgccgaa ggtttatccc gacgcgcatt
     2341 gcgagctcga tttccggacg cccttgcaac tcctgatcgc gaccattctc tccgcgcaat
     2401 gcaccgacaa gcgggtcaac ctggtgacgc cggcgctctt cgcccgttat cggacggcag
     2461 cggattacgc ggcggcgcca ccggcggaat tggaaaaggc gatccaatcg accggcttct
     2521 tccgaaacaa gaccaaaagc atccgagccg cgaccgcggc gattgtgcgg gagcacggcg
     2581 gccgggtgcc gcagaccatg gccgagctgc acgcgctccc cggggtgggc cggaagacgg
     2641 ccaacgtggt tctcggcaac gccttccata aggacgaggg cgtggtcgtc gatactcacg
     2701 tggcgcgtct ttcggagcga ctcggcctaa cgaatcagaa agagccggag aaaatcgagc
     2761 gcgacctgat gcggctgatc cccaaaaaac attggaccaa ctggagccac tggctcatct
     2821 ggcacgggcg ccggcgctgt ttcgcgcgcc agcccgattg cgcgcactgc gaattgttcc
     2881 agctttgccc cgcgggtaaa aaattcatcc gcgcaggaac ggcgcgggct tcagtctgac
     2941 ccggtcttgc ggctcatctt gctcaccgcc gcggcggttg ccttcgagcg tttccggagc
     3001 cgctcgaact ctttctgaaa ttcttcgaga tcgtgatccg agagagtttc caaatccatc
     3061 aggcgattgc gggcgccggt catgctgcgg atgcttgcct ggcgatagta attattgaat
     3121 cgctttaacg gtgcggcctg cgcgctcggt gcgttttttt ctcatgtata caatgtccga
     3181 tgcggtcctt catctgaatc tcgatgatgc ctggcatttg cccacgctcg cgttgccgag
     3241 cgccgacgcc cgcgattggg ggccacggct gcgcttctcc gcgcggccgc gcgaaattga
     3301 gcaatttcag cgcgagtggg tccagcgttt gccgcctttt ctcgtttacg gttccggcga
     3361 ttttcatcac ctgacggcgc tctggctccg ccggctgact cgcctaacga cggtcgtctc
     3421 gttcgataat catcctgact gggacattcg accgccgcgg tggtgctgcg gtggctgggt
     3481 caatcgcgcg ctcgagttgc cgcaggtgga aaaagtgagc gtgtggggtt gcggcaactt
     3541 cgaatgctgg tggcccggcc ggatctttgg caacaaccgg gccgaacgcg ccggaagtct
     3601 ggaagtgcat ccttgggcgg atgacagaag cgcaaaggag cggcagcgtc gcggcgcgat
     3661 cctgcgccac aactggcggg aaaaatttgc ccgctttgtc gagcagcttg gcgccgcgca
     3721 ggtttacgtg acggtcgatc tcgattgcct ccgcgcggaa gacgcggtcg cgaattggga
     3781 aagcggccgt ttcaggaccg aggatgttgc ctgggccatc gagtcgttgg gaaagggcgg
     3841 ccgcatcgtc gcgggcgaca tctgcggcgc gtggtcgccc gccctttatg cgcggcggaa
     3901 acagcgcttc gcttccgaaa tggaccatcc gaagcttcct ccgcgcgatc cggccgaagc
     3961 ccggcggacc aacgaggccg cgctgcgccg tttgctgccg gctctaactg agcgaaacga
     4021 ggaccactcc cagggtgatg atcaacgagc cgatcgttag gcgaagggtc aatttctcct
     4081 tcaggacgaa cgcgccggcg agggcaatca ggatcacgct caggccctga aaagggaaga
     4141 aatagctgag gtcgaagcgc tggagcagtc cgatattgac aaagaaattc actgcaaaga
     4201 cggcgatggc ggcggcgaaa acgagcgtcg cgtggcctcg ggaataattc gcacgcgccg
     4261 tgatgtccat cgcgtgcttg agcaggatct gcccggagac aaagcagccg acggtgacga
     4321 aaatgaggag ggcggcgaaa ggtgtcacaa gcgttcctcc agcttcgcgg cgggcttggc
     4381 catgatcatc aggccgagca cgagaagcgc gatcccaaac cagcgccggg tcacgatcgc
     4441 ctccccgaga aagatccaac tgccgagcgg aacgaagatg tgcacgacgt tgccgaccgg
     4501 aaacgcgatc gtgagcggga gtttccgcag ggtggcaagc caattaaaaa gactaacgac
     4561 cgaagcgatg atgccccacc agacccaggc cgagcgcagt cctgtcagcc cagtccacga
     4621 ccaggcgttc gccggattcg cggtctccac cgcgcccagt ttaagaaata attcggcggc
     4681 cgtgccgagg aggacgcaga tcgacaactg gatccacgga ttcaaaaacc agcgccgaat
     4741 caggcccggc cccgccggtt ccgctgtgct ccgcatttag tgcaccgtct cggcgttgcc
     4801 tctgatagag tgcactaaag ctcgtgcgcg ttggcgaact gctgcagcac cggatcgtgt
     4861 ttggtcggct ggaggaagcg gagcgcgtgg cggaagatcg gattgagcaa cgcgccggtg
     4921 tgcagcacgt aaagatcgag cgggacgagc tcgcagccga ggtgcagctt gggatcgtaa
     4981 ttaagcgggc tgctgcagta ggagtgcaga ccttgcgcga gcgcccaggt gatgatgtcg
     5041 cgcatcgtgt aaaaatacag atggagatcg aaggcgaccc ggtaatcgag cccaaggtac
     5101 tcatcgtaaa tcgtgccgtc gtgcaccagg caaaggctga aagcgatcgc cttgccttcc
     5161 tgccgccaga cgaagaagcg cgtccgttcc ggcatctccc ggcccagccg acaaaaatac
     5221 tcgggcgtca gcctctcgaa acgcaacttc gagcgttcat ggacctggag gtaaagggga
     5281 taaagctcgt cgatcaccgg cgtgacgtcg gtcagcacct cgcactgaat gggcgcgctt
     5341 tgggccgtct tgcgaaactt gcgacgcaga ttcttgcggg tcgccttgcc taacgacgcg
     5401 acatattcgt cgaagttgcg gtagtgcagg gagagtcgtg tcatcggcat gctggcgatc
     5461 cgagtgtagc cgttggcgga gaagttgcgg agcggcgcgc gataacgcgc cgggaaatcc
     5521 ttcaacacca cgagcgaggc gcgggcctca cgggcaaaca cttttagaca ggaatggagc
     5581 gcttccgcca cccattctgc atcttcggga cggcatgccc cgagatggcc ttcgcccgcg
     5641 gtgctgccga ccatcagcgc cttcatggtg aggaaacgcg gaaacttgcg ccggatccgg
     5701 tcgaccgtag cgcgtatcgg accggccacg ccttcggtca gattctggcg cacgaaaaag
     5761 agcggctgaa tcgctcgcac ccgccccgca ttatcttcga gaacgaggaa aaaataatcg
     5821 aagttgtcgg tcagggtcgt cccgatcagc tcgtaataac ggtggtcctt gcactgatcg
     5881 agaaacgctt cacgccaggc ggggcaatcc tgcaattccg cctgggtgag aatcgtggca
     5941 aagccctgcg gaatccggac ggacgtgccg gatggcccgc gttttagccg tcgtgaagag
     6001 aggatgggag cgccgatacg aatatgtcca gtagcaagag tgcgcgaaat ttcaagcgca
     6061 gagccgcgcg tgtctcggat ttcctcattc cgtccgggtt atcggcgcag gtttgcccgg
     6121 cgcggattcc accttcaaaa gaccactctt aacatggagg tcgcgctgcg ggaccgggat
     6181 ttcgatcccg gctgcgccga gtttccggtg gatcgcgaaa ttcaggtcgc tgcggaaccg
     6241 attgggccgg ttgctcattt cgttgctcca gacgacgagt tcgaatttga gcgagctgtc
     6301 gccgaatccg acgaagaaaa cgctcggcgc cgggttctcc aggacatgcg ggtcttcctg
     6361 ccccgcctcg atcagcgtcc ggcggacctt ttccacgtcg cttccatagg ccactccgac
     6421 tggaatccgg aagcgcaccc ggggatcgcc ataagtccag ttcgtgaccg gtgaatcgat
     6481 gaacttctgg ttaggcacga tggtggtgat gttgtcattc gtcaccacca cggtgcagcg
     6541 cgcccgaatt ttctgcacct gaccggtgac gccggcgact tccacgcggt caccgatcgc
     6601 aatcggccgc tccgccagaa tgacgagacc gctgatgaaa ttgctcgtga tattttgcag
     6661 accgaagccg atgccgacac cgatcgcgcc ggcgaagacc gtgagcgctt cgagatgaat
     6721 accggtgttt tggaggacga tgaaaatacc gacgacgagg acgacgttgg aagcgatttg
     6781 cgagatcgcg tactggagcg agcgatcgag tccgctgtgg acgaggaagc gattaaagag
     6841 aaagcgtttg gtgcgcgcag agagccagaa gaccaggatg agaaggccga cgaggagaaa
     6901 gagctggaga agactgattg ggattccggg cagcgggtcg ctccaggcga gggggatgcc
     6961 gagaccgtta atcgcgccca cgaagaaaaa gacgagcgcg gcgagactga gaatcgtcgt
     7021 gatgatcgcg acgaagttgg cctcgagttt gaggcggctc aggaaacgcc gcacgaccgc
     7081 gctctggagg gccttggcgg agatcagccc gagcgccatc agcccgacga aggccacgat
     7141 cccgaggagg gagatgtggt agcgcccgag atcaaacaag ggggtgctta accaaaggag
     7201 attcaccctg ccattctctc tcgggggacg gcgctctgca aagcacgcaa caaaaaacac
     7261 ggccgaggcc gtgtttcaag ttgcgtgggc aagcacgctg agagacagcg gagcggcttt
     7321 attctttggc tgccttgtcc tcgatgtatt tcaccacgtc gcccacagtc tgcagcttct
     7381 ctgcatcttc atcgggcact tccacggaaa attcctcttc aaaggccatc accaactcga
     7441 cgatgtcgag cgagtccgct cccaagtcct cgatgaacga ggcatttggg gtcacctgct
     7501 ccggattcac gcctaattgt tcgacgatga tgtccttcac tttttcttcg accggtttat
     7561 ccgacataca aattattctc cttggttcga ttctgtaggg tgacggctta aaggcgtcgc
     7621 ggcaagttgg caactaaatt aattggattg gttgtcaatt tattttgcac gatgaagccg
     7681 gttggagtcg gcaaaacgcg ccgcggattt tacaaccgcc cgccttcgcg taaagactct
     7741 gatgcccgag gaaaacgacg cgccggatct tttggatctg aagtttctgc ccgcctgggt
     7801 gaaggagacg cctaacgaga atctctatgc ccattttgcc ggcgaagagg ctgcggcggt
     7861 cggtcaacgc gaccgtcgtg gtccggatcg gcggggcggc gagcggggcg agcgtggccc
     7921 gcggccgacc cgggagaaag gaagccgacg cgacccgcgc gagcgcgccc gcgggcccgc
     7981 ggatcagcgg ccgcgcgagc cccggagacc ggaggaaccg gccccgcccg cgccggccgt
     8041 cgaagtgcgc tttgtgccgg atgcgcgggt cctggaaaat gtccttgcgc aaatcaaggc
     8101 gggccatctc gcctactcgg tcttttcgct ggcgcgaatg tttctcgaga aaccggagcg
     8161 ctacgatgtg cgtttgaaag cggaaacggc tgcacttttt cagctcggcg aaaacggtca
     8221 ggtcgcgagc gaccagcgag tgctggagaa cggtgcgttc ctcaacgaaa aggatcactt
     8281 ttatcggacc gaggtgacgc agtctgaccc gatcaagggc aatttcacta ccgtcgcgcg
     8341 ctgccgcttg agcggggttt tgttaggccc gaccaaccat cacgcttatc agccgcaact
     8401 gcgcaatctc tacgaacagc gtttcagccg gcgaatgagt tttcaggatt accagcggca
     8461 ggtcgagatt gtgagcaacc ccgaggcggt cgaacgctgg aaggaggaag cgcgccaggt
     8521 gaccacctat gtgacgttgc aggaggagcc gccgattact ttccagaatc cagcggagac
     8581 cgaacgccat tttcgccaga cctatctccc tggcctgctc cgtcagagct tggaattgac
     8641 gctcgacggc gtcgttagtc gccggctggc cgatcgctcg ttaggccggg tgatcgagga
     8701 cgggtgggcc caggaagctc gctcaccgac gaagatcatg caggaattga tcggcgcttt
     8761 tcgccagggc gggctgcaca ttttccggca tcgcaaggcg atgctcttcg tctcgcccat
     8821 tcgcgcgcga ccctttcacc acgagagctc ctcggtctcc tcgaccgtag cggccatcct
     8881 tgcggcgttg agcgccaatc ccgggataaa tcgcaagcag ttgctggaga gattgccgcc
     8941 tgacaccgcc ggggaaccgg agagcgtcga gaagaagaaa ctggcgctcg cctccgatct
     9001 gcactggctg atcaacgaag ggcacgtgat agaattcaac gacggctcgc tcgacctgac
     9061 gcgaaccaag gctccgcctg caaatgctcg agcgcaggcc cgctccgctg cgcctaacgg
     9121 gatcgcggaa ggtctcgctt cgcctaacga ctcgaagaac acgagcgaga cgagcgcggc
     9181 ccccgcggtc gaggaacatc ccgcggagat tgcgcgcgag ggggacagag tgccggaggg
     9241 agaagcgttg ccgccggcgg tggcggagat tacgccagat ggcgcacccg aaggttcgtc
     9301 cccggcgaag aacgctctgg gcgcgggcta agccgcgatc agcccggctt ttttcaacgt
     9361 cgcgtaggcg ccgtttttgt tgatcgtctt caagccgcgc gcggttgtct tcacgcgcac
     9421 gaatttcttc agctccgcga cccagatccg ttgttcgcgc agattgggtt gaaaggtgcg
     9481 cggcacgttt ttggtcacgt gacggcccac gccgcccttc ttcttggcca tgccgcgacg
     9541 gtggatcacg ctgccgcggg aaggtttcga tccagtgatg ctgcaaactt ttgacataaa
     9601 ccggtttgag aggggcgtaa gctgccgtcg gtggccagag cggtcaagcg attccttgcc
     9661 cgcgggagct ttggacttca cgaagcgaat ccttcgtcgc aggggtcggt gaacgcggtc
     9721 acacgcgatg gcggcgacgc gcctcctgcc gggaatcccg gctccaaccc tggctcgtgc
     9781 tgtaagcgaa ccgacgaaca gagagccaac gaacgctgcc ggagttcact cgcgaaagca
     9841 ttcggagttc actcgcgaac gcattcaggg gctcactcgc gaattctttc ggagttgaac
     9901 agcggccgcc aaccgtggtc aaatacgcct ctccccggat ttcgcgcgat cgggaaccgt
     9961 cttatgagcc acaaaacgaa tcacaagctg cggctcggaa ttgtcggggc aggagaagtc
    10021 gtgcgccggc gccactggcc ggcgctgcaa cgccagtcgg acatcgaact ggtcgcggtt
    10081 tgtaactcgt cctacgaaag cgcggagaag ttctgccgcg aaaacgcgcc gcacgccacg
    10141 ccgatgcaga actgggcgga attgctgggc atggccgatc tcgacattgt ctggatcggg
    10201 actccgccct acatgcattc cgccgttacg gtttcggcgc tcgaggcggg gaaacacgtt
    10261 ttttgccagg cgcgcatggc gatggatctg gccgaggcgg aggaaatgct ctcggtctcg
    10321 cgccgctttc ccgaactggt gacgatgctc tgcccgccac cccacgggat gcgcggggat
    10381 ctgctcgtcc aaaaattact cgcggagaaa actctcggcc gcccatacca cttgcgcctt
    10441 cagagtctcg acagcgcctt tctcgacccc gatgcgccgg cgcactggcg gcagcgcgtg
    10501 gaaatgagcg gcctcaacac gctcaccctc gggatctacg tcgaggtctt gcaacactgg
    10561 ttcggccaga tcactggcgt tttcgcacgc ggcaggattc tgcagccgat gcggcagggt
    10621 tacgaagtca tcattccaga cctgctgacc gttctctgca ctttcgccaa cgggatggaa
    10681 ggtgtcctcg aatttagcgg cgtcgacgcg cacgcaccgg cggaccgatt ggaggtttac
    10741 ggcgacctcg gcacgctgac ctacgatttc ggtgccgacg ccgtgcacgc cggacagatt
    10801 ggcgatcgcg cgctgcacgt cgtcgagttg acccccgacc tggagactaa ctggcatgtg
    10861 gaggatgact tcatcgcggc ggtgaaatcg aggggtcgca tcaatccgca tccggatttc
    10921 gaggatggcg tgcgctacat gcgcgtcgtg caggccgtgg ccgactctcg cgcgcgcggc
    10981 gaatgggtcg cgatcaacga tcgcgcctaa cgaaggcggg cgccctcata acatcgcgcc
    11041 ccgcatgatg aggagcgcga tgctgaagta aatgatcagc ccggtgacgt cgaccagggt
    11101 cgcgacgaag ggcgcggatg aagtcgccgg gtcggcgcca agccgccgca aaagaaaggg
    11161 cagcatggag cccgcgagag agccccagag caccaccccg acgagggccg cgccgacggt
    11221 caaggcgacg agcggccaat gcggcccgta aagactgcga tgaaaatatt gctccccgac
    11281 gatcgaccag acggcgaccc gcaccgtgcc gatcaccccg agaatcaagc ctaacgataa
    11341 gccggtctgt aattcccggc tggccacccg ccaccaatcg cgcaacgtca cctctcccag
    11401 ggccatcgcg cggatgatca aggtcgacgc ctgcgagccg gagttgccgc cactcgagat
    11461 gatcaaaggg aggaagagcg ccagcacgac cgccttcgcg atttcatcct cgtagtttgc
    11521 catcgccgtc gccgtcagca tttcgccgag aaacagaata accagccagc ccgcccgctt
    11581 ctgcaccatt cgccagagcg ggatgcgcat ataaggctcg tccaacgcct ccatgccgcc
    11641 gaatttctgg atgtcctcgg tcgcttcctc ctccgcgacg tcgagcatgt cgtcgatcgt
    11701 caccactccc accagcaccc cggtggaatc gaccacgggc agcgcggtcc ggtcatactt
    11761 gcggaagacg ttgaccgctt cctcctgcga gtcggtcacg ttcagagcga tgaaattctc
    11821 atccatcaga tcgctcacct tcgcagccag cggcttgagg aggaactcgc gaatccgaat
    11881 gtcgtcgacc agctttcccc ggtcgtcgac gacgtagacg acgttcaacg tctccgagtc
    11941 ctggccgtgc tcgcgaatgt aatcaagcac ctccttgacc gtccaattgg cgtcggccgc
    12001 gaccaggtcc ggcgtcatca aacgtccgac actgccctcc ggataaccga gcaacgcctg
    12061 cgcgatccgt cgttcctccg gcgtcagcag acggatcagc tggcgcgcgg cagcgctcgg
    12121 caattcttcg agcaacgccg tgcgatcgtc cggcgacatt tcgttgagga tcgaaacgac
    12181 ctgctcatgg gccatcccgc ggagcagttt ttgttgcgcc tcgacatcga cggcgaggta
    12241 ttcaaaaacg tccgcggcga gcgcgtgcgg caggatgcgg aagatgatta cctgttcgtc
    12301 ttccggcagg tcgagaatga tctcggccac gtccgccggt ggcatctcgg aaaagagttc
    12361 gcgcagcgcg gagaaattcc gcgcgtcgat caggctctg
//
LOCUS       DATMNS010000229         4274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4468135, whole genome shotgun sequence.
ACCESSION   DATMNS010000229 DATMNS010000000
VERSION     DATMNS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4468135"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /gene="queG"
                     /locus_tag="VNW28_08095"
     CDS             <1..576
                     /gene="queG"
                     /locus_tag="VNW28_08095"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HXA09930.1"
                     /translation="WFFLAEIFTTLELASDPPQVARCGSCTRCIDACPTAAITAPHQL
                     DARRCISYLTIELKGSIPLEFRPLIGDRIYGCDDCLDACPWNRFAQVARENAFAARPA
                     VVQMRLRDFLALDDAQFREMFRGSPIKRIKRRGFLRNVCVALGNAGTPEDLPALRKAA
                     QDDEPLIAEHARWAIERIETRLSRPLCAPRL"
     gene            740..2092
                     /locus_tag="VNW28_08100"
     CDS             740..2092
                     /locus_tag="VNW28_08100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXA09931.1"
                     /translation="MHQAAAPSPTVRRKRKSRRKLYIWLGVGGLIFLLIIGSVISSKR
                     EKPIEITTEKATRRTIVETVSATGKVQPETEVKISPEVAGEIIELPVEDGKEVKKDDL
                     LLKIRPDSYKAILEQQQAAISTAQSVSGQQKAAMTKAEQDLAQAQDLFHKKLISQTEM
                     LAAQTTYDAAKSMYQSGLHSIEGAQASSSQARDQLSKTTIYSPLDGIVTVLNSKLGER
                     VVATNQFAGTEVMRVADLNHMQAVVDVNENDVVHVKLGDSAKVQIDAYGDRKFSGKVE
                     QIANTGTTTGTGTQEEVTNFQVKIQLDKPGVRLRPGLSCTAEIQTSMVKDVVAVPMQS
                     VTIRTGDSNLSPEEIEQKKKLAAAKENADNNAQVENERLAKQEKKEEREKLRKVIFLK
                     DGSKAKMVEVTTGIADDASVEIKSGVKPGDEVISGSYSAISRKLKDGAKVVMEKVEKK
                     "
     gene            2183..2929
                     /locus_tag="VNW28_08105"
     CDS             2183..2929
                     /locus_tag="VNW28_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA09932.1"
                     /translation="MNISEGMRAPGPLVIDIENITKEYVMGEEIIHALRGVSLQIRRN
                     EYLAIMGPSGSGKSTLMNMLGCLDTPSSGRYEFNGKNVSEMDGDELATIRNREIGFVF
                     QTFNLLPRSTSLRNVELPLIYAGIDPEEREERATQALADVGLGDRLTHKPNELSGGQR
                     QRVAIARALVNHPSIILADEPTGNLDSKTGEEIMALFESLYQQGNTVILVTHEADIAR
                     HARRRVHLRDGVVESDELAAVATGSPVAVS"
     gene            3029..>4274
                     /locus_tag="VNW28_08110"
     CDS             3029..>4274
                     /locus_tag="VNW28_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA09933.1"
                     /translation="MKRFLYELTESWKIAAAQMAANKTRSMLTALGVIIGIVAVTLMG
                     TAINGISTGFDKSMAVIGDDVLYISQRPWTQMNDWWNYRDRKKIRTEYADKINHAIAT
                     TPNSNLMIAVPTMSLLRSVKAGDNSVDGVFILGTTTDYTLISTLDCSEGRFFNELESR
                     GGAEVCVVGFDVADAAFAGRSALEKKVLINGHAFKVIGTLSRQGTFLGLFSLDSIVVI
                     PLPAFKKYFSAKSEADVRVKVRDKTHMADAIDELTGIMRRLRSLPPEKKDDFSINEQQ
                     AFKSTLDAVKTTIAIAGLFITGLSLFVGAIGIMNITFVSVKERTKEIGTRKALGARRR
                     TILLQFLIESTALCLLGGVIGLTVAYLLCFGIGKAFPSFPIQFSFGLVLASMLVSILT
                     GLFSGFAPAWSASRLDPVTALRY"
BASE COUNT          956 a         1302 c         1258 g          758 t
ORIGIN      
        1 tggtttttcc tcgcggaaat ttttaccacg ctcgaactgg cgtccgatcc accgcaggtg
       61 gcgcgttgcg gatcgtgcac gcgctgcatc gatgcctgcc cgaccgccgc catcaccgcg
      121 ccacatcagc tcgacgcgcg ccgctgcatt tcctacctga cgatcgagct caaggggagc
      181 atcccgctcg agttccggcc gttgattggc gatcggattt acggctgcga cgattgtctc
      241 gatgcctgcc catggaatcg cttcgcgcag gtggcgcgcg agaacgcgtt cgcggcgcgg
      301 cccgcggtcg tgcaaatgcg gttgcgcgat tttctcgcgc tcgatgacgc gcagtttcgg
      361 gaaatgtttc gcggctcgcc gatcaagcgg atcaagcgtc gcggcttctt gcgcaacgtt
      421 tgtgtcgcgc tcgggaacgc gggcacgccg gaagatctgc cggcgctgcg taaggcggca
      481 caggacgacg agccgctcat cgcggagcac gccaggtggg cgatcgaacg gatcgaaacg
      541 cgcttgagtc gtccgttgtg cgcgccgcga ttgtagggcg ggcgctccgc ctgccgtccc
      601 ataacgatga tgcgcggatg gcacgcgaag cgcttgccct acatggacgt tctggcgcgc
      661 gtctggtttc ggattgaaca gggaaatatt tgcaaaggtc taaagagccg ctaattctga
      721 gcccttcacc cgtcttccga tgcaccaagc cgccgcgccc tcacccaccg ttcgccgcaa
      781 gcgcaaatcc cgtcgtaaac tctatatctg gctcggcgta ggcggattga ttttcctcct
      841 gatcatcggc tccgtcatct cgagcaagcg cgagaagccg atcgagatca cgaccgaaaa
      901 agcgacccgc cgcacgatcg tcgaaaccgt ttcggcgact ggcaaagtgc aaccggaaac
      961 ggaagtaaag atttcgcccg aggtggccgg agagatcatt gagctgccgg tcgaggacgg
     1021 caaggaagtg aaaaaagacg atctgctttt gaaaatccgg cccgactcct acaaagccat
     1081 cctcgaacag caacaggccg cgattagcac cgcgcagtcg gttagcggac agcagaaggc
     1141 ggcgatgaca aaagccgagc aggacctcgc gcaagcgcag gatcttttcc acaagaagct
     1201 gatctctcaa accgaaatgc tcgctgcgca gaccacctac gacgcggcga aatcgatgta
     1261 tcaatcgggc ctgcacagta tcgagggcgc gcaggccagc tcgagccagg cgcgcgatca
     1321 gctttcgaaa acgaccatct attcgccgct cgacggcatc gtcacggtcc tcaacagcaa
     1381 gctcggcgaa cgcgtcgtgg cgacgaatca atttgccggc actgaagtca tgcgggtggc
     1441 cgatctcaac cacatgcaag ctgtcgtgga cgtgaatgag aacgacgttg tgcacgtcaa
     1501 actgggcgat tcggcgaagg tccagatcga cgcctacggc gaccggaaat tcagtggcaa
     1561 agtcgagcag atcgccaaca ccggcacgac gaccggcacc ggcacgcagg aggaagtgac
     1621 caatttccag gtcaaaatcc agctcgacaa acccggcgtc aggctccgtc cgggcctcag
     1681 ttgcacggcc gagatccaga ccagcatggt gaaagacgtc gtcgccgtcc cgatgcaaag
     1741 cgtcaccatt cggaccggcg acagcaacct cagcccggaa gagatcgaac agaaaaagaa
     1801 actggcggcg gccaaagaaa acgccgacaa caacgcccag gtcgagaacg aacgcctcgc
     1861 caagcaggag aaaaaagaag agcgcgagaa gctgcgcaag gtcattttcc tgaaagacgg
     1921 cagcaaggcg aaaatggtgg aggtcacgac cgggatcgcg gatgacgcga gcgtcgagat
     1981 caagagcggt gtgaaaccgg gcgacgaagt gatctcgggc agctacagcg cgatcagccg
     2041 caagctcaag gacggcgcca aagtggtgat ggagaaggtg gagaagaagt gagcgtttta
     2101 accgcagaga tcgcagaggt cgcagagaat tacatgaatc agaatttttc ctctgcgctc
     2161 tctgcgtcct ctgcggttaa acatgaacat ttctgaaggc atgcgagccc ccgggccgct
     2221 cgtcatcgac atcgagaaca tcacgaagga gtacgtgatg ggcgaggaaa tcattcatgc
     2281 cctgcgcggc gtgagtttgc agatccggcg caacgaatac ctcgccatca tgggtccgag
     2341 cggaagcggc aaatcgaccc tcatgaacat gctgggttgt ctcgatacgc cgagttccgg
     2401 gcgctacgag ttcaacggca aaaatgtctc cgagatggat ggcgacgaac tggccacgat
     2461 ccgcaatcgc gagatcggtt tcgttttcca aaccttcaac ctgctccccc gctccaccag
     2521 tttgcgcaac gtcgagctgc cgctgattta cgccgggatc gatcccgaag agcgcgagga
     2581 gcgcgcgacc caagcgctgg ccgacgtcgg gctgggcgac cgcctgacgc acaagcctaa
     2641 cgaactgtcc ggcggccagc gccagcgcgt tgccatcgcc cgcgcactgg tcaatcatcc
     2701 ctcgatcatt ctggccgatg agcccaccgg gaacctcgat tccaagaccg gcgaagaaat
     2761 catggcgctc ttcgaaagcc tctatcaaca gggcaacacc gtcattctcg tcacgcacga
     2821 ggccgacatt gcccgccatg cccggcgccg cgtgcacctg cgcgacggcg tggtcgagag
     2881 cgacgagctg gccgcggtcg ccacggggag cccggtggct gtttcttaac cgcagaggac
     2941 acagattgca cagaggaact aaattctttg ttccaaaaaa actctgcgtc ctctgcgctc
     3001 tccacggtta atattttccc ggcacagtat gaaacgcttt ctctacgagc tgaccgaaag
     3061 ctggaagatc gccgccgccc aaatggcggc gaacaagacg cgctccatgc tgaccgcgct
     3121 cggcgtcatc atcgggatcg tggcggtcac gctgatgggg accgcgatca atggcatttc
     3181 caccggcttc gacaaaagca tggccgtgat cggcgacgac gtgctctaca tctcgcagcg
     3241 accctggacg caaatgaacg actggtggaa ctaccgcgac cgcaaaaaaa tccggaccga
     3301 gtacgcagac aaaatcaacc acgcgatcgc cacgacgccc aactccaacc tgatgattgc
     3361 ggtgccgacg atgagtctgc tgcgcagtgt gaaagcgggc gacaacagcg tggacggcgt
     3421 cttcatcctc ggcacgacca ccgattacac tttgatctcg acgctcgatt gcagcgaagg
     3481 ccgcttcttt aacgagctgg aatcgcgcgg cggagcggaa gtctgcgtcg tcggctttga
     3541 cgtggccgac gcagccttcg ccggacgcag cgcgctggag aagaaagttc tgattaacgg
     3601 ccacgccttc aaagtgatcg ggacgctctc ccgccagggc acctttctcg gactattcag
     3661 tctcgattcg atcgtggtca tcccgctgcc cgcgttcaaa aaatatttca gcgccaagag
     3721 cgaagcggat gtgcgagtca aggtgcgcga caagacacac atggcggacg cgatcgacga
     3781 gctgaccggt atcatgcgcc ggctgcgttc cctgccgccc gagaagaaag acgatttcag
     3841 catcaacgaa caacaggcct tcaagagcac cctcgacgcg gtgaaaacaa ccatcgcgat
     3901 cgccggcctc ttcatcaccg gcctgtcgct ttttgtcggg gcgatcggga tcatgaatat
     3961 caccttcgtg agcgtgaagg agcgaacgaa agagatcggg acgcgcaaag cgctcggcgc
     4021 gcgccggcgc accattctcc tgcaatttct gatcgaatcg accgcgctct gcctgctcgg
     4081 tggcgtgatc ggcctgacgg tcgcttatct gttatgcttc ggaatcggca aagccttccc
     4141 gtcgttcccg atccagtttt ccttcgggct cgtcctcgcc agcatgctgg tctcgattct
     4201 gaccggattg ttttccggct tcgccccggc ttggagcgcg tcaaggctcg atccggtcac
     4261 agcgctgcgg tatg
//
LOCUS       DATMNS010000230         6395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4468683, whole genome shotgun sequence.
ACCESSION   DATMNS010000230 DATMNS010000000
VERSION     DATMNS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6395
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4468683"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VNW28_08115"
     CDS             complement(<1..231)
                     /locus_tag="VNW28_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09934.1"
                     /translation="MKQRLAILFLCSAFCAATAQAAEREYIVVVGGPSLHIWEQYKGP
                     VAHDHWWANFVHSARVRTEQLRAQLGPDAMITW"
     gene            complement(285..1091)
                     /locus_tag="VNW28_08120"
     CDS             complement(285..1091)
                     /locus_tag="VNW28_08120"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HXA09935.1"
                     /translation="MFWPVVACVLLPWLLVYLGLHVVRRGIIFIDIAMAQMASLGICV
                     AVLLHLNLEGWPAFLIALGFTLVGAAIFSVTGKRTSQIPQEAVIGISYVVAAAAAVLL
                     LSRAAEGDEEIKNMLVGNILLVSPLDVWKCFGLFLSVGVVHFVLRRSFLLVSFERDTA
                     YERGLRVRWWDFLFYALFGLVVTSFVRIAGVLLVFSYLIVPAVCGISLARSLGRRLLI
                     GWFVALAGGVAGLFLSFQWDLPSGAAIVCTFGVLLILIGSVGLLLRRRAD"
     gene            complement(1279..2187)
                     /locus_tag="VNW28_08125"
     CDS             complement(1279..2187)
                     /locus_tag="VNW28_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HXA09936.1"
                     /translation="MKKIKLLCSGGALFAGLLLSAEAKINVVATLSDYGSIAREIGGN
                     NADVTVLAKPTEDPHFVDARPSFVVKLRQADVLIDGGAELEIGWLPPLLQNARNPKIE
                     AGQPGRVEASQGIRLMEVPAQLTRAAGDVHAMGNPHFMVDPIIAKAVAQHIANAFAAV
                     DPAHAADYQANEKKFEATINAKLQEWGAKLLPFSGQSLVAYHDSWVYFAHRFGFNISV
                     FLEPKPGIPPSPSHLAEVIAQMKAQKIRAIIVEPYHNRKIAEKVAESTGAKVVDFAQF
                     PGALPNTDTYVQLVDALVNRLAAALK"
     gene            complement(2192..3772)
                     /locus_tag="VNW28_08130"
     CDS             complement(2192..3772)
                     /locus_tag="VNW28_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09937.1"
                     /translation="MKKLFIVLAALGAQAAVFAQSPSPAPSPNDVEVLRQQVQALTET
                     VKALQQQVKEQQDTLAKMNPEPGTLAANQSPAATPQPAASAPPLFPTTDESVVASAPQ
                     SSAPPAPNSPAAVPVNGSFPTTDASVTTSPETISSTGAGASLTAPITIAGGGKTYMNL
                     SFDSVFSLAYSSDRDLDQLEVGDHDPQQRGFNARNAEIALDGAVDPYFEGFANIVLKL
                     NNDNETEIELEEAFMQTTDLPWGLQVKGGQFFSAFGRINPTHPHTWDFADDPLVHGRF
                     LGSDGLRGVGAQISWTVPVAWYSQFVFGVQNGRGNTGYSFRNPGDNGVFYGRLTTDRE
                     ARGLQDFVYVPRWENSVDLSPTQTILAGVSGAFGSNDTGANTRTQIYGADLLYKWKSP
                     RAEGGFPFVKWQSEAMYRRFEAGRGVDDSFPVAETFHDWGMYSQIIWGFKKGWTAGVR
                     GDYLHMEDSAFTDDPARQSRSRVSGDMTWYPTEFSKIRLQYNHDFLEANDFLAAREAD
                     SLFFQFEFILGAHGAHKF"
     gene            complement(3914..4174)
                     /locus_tag="VNW28_08135"
     CDS             complement(3914..4174)
                     /locus_tag="VNW28_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09938.1"
                     /translation="MGAFLFAIALSDAPRLHEHFHKLLGADHECAVTIVLSGACDHLA
                     GGAPGAAPVALLSTSVFVPQYLQPLAAGLEFSRLEHAPPALA"
     gene            complement(4308..5615)
                     /gene="chrA"
                     /locus_tag="VNW28_08140"
     CDS             complement(4308..5615)
                     /gene="chrA"
                     /locus_tag="VNW28_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HXA09939.1"
                     /translation="MVERQHPSFGEAFRFWLRLGFISFGGPTGQIAIMQTELVEKKRW
                     ISQARFLHALNYCMLLPGPEAQQLATYIGWLLHKTWGGIVAGAFFVIPSIFILWGLSF
                     VYAAYGNLPWIAAIFYGLKPAVMAIVAVAVIRIGRKALRNEVMWTLAALAFVAIFFFK
                     VPFPLIILGAGLIGFLGGYLWKSKFDVVSGHAKAGNTSVLDDETESPAHTQPSFPRAI
                     KVTLVWLVLWLAPVMAVGLWLGWNHTLFQEGIFFSKAAVVTFGGAYAVLPYVTQQALF
                     HYGWLKPGQMMDGLGLAETTPGPLIMVLQFVGFMGGWQHPQGLSPLFAATVGALLTTW
                     VTFTPCFLWIFLGGPYIEKLRGNIKLTTALSAITAAVVGVVLNLAVWFALNALFPKHH
                     VVDWFALTITIVAFVGMLKWRWDVVPVVVGSGVLGLLYEFALH"
     gene            complement(5648..>6395)
                     /locus_tag="VNW28_08145"
     CDS             complement(5648..>6395)
                     /locus_tag="VNW28_08145"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HXA09940.1"
                     /translation="KKIRDSGGDCTLVRAQEIESLTQEKVISFFNSARDQEYVALKKT
                     LQSFLTKRKKSSAESAPSELERLTRQFRELREIDFFDSPRGHEVAMLLRRAEETPRKT
                     LPRLDSKDYRGKNWQTRPRPEIDRVGSAWLICKFIDPEAKFVFASDPPLHPGIIPFDM
                     LDVEFSHHDAFCTFETLIRRFDIDDKAVRKIGEMIHDADLDDAKFQRVECIGIDRVLK
                     GWGKSPMTDQEILTAGFACFDGLYEFLQRR"
BASE COUNT         1387 a         1882 c         1958 g         1168 t
ORIGIN      
        1 ccaggtgatc atcgcgtcgg gaccgagctg ggcgcgcaac tgctcggtgc gcacccgcgc
       61 cgaatgaaca aaattcgccc accaatgatc gtgcgcgacc gggcctttgt attgctccca
      121 gatgtgaagc gacgggccgc ccacgacgac aatgtattcg cgttccgccg cctgggccgt
      181 ggccgcacaa aaggcgctgc aaagaaaaag aatggcgagc cgctgtttca tgatttgtcg
      241 cgagcactat ctatgcgcgg aacggaagtg cgcgcaaggg aggatcaatc cgcccggcga
      301 cgcaggagca ggcccaccga cccaatgagg atgaggagca cgccgaaggt gcaaacgatc
      361 gccgcgcctg acggcaggtc ccattgaaag gagaggaata acccggcgac gccacccgcg
      421 agggcgacga accagccgat gaggagacgc ctgcctaacg aacgggcaag actgatgccg
      481 cagaccgccg gcacgatgag gtaggaaaaa acgaggagca cgccggcgat gcggacgaaa
      541 ctggtgacga ccagaccaaa gagggcatag aacaggaaat cccaccagcg cacccgcaag
      601 ccgcgctcat aggcggtgtc gcgctcgaaa gagacgagca gaaacgagcg gcgcagcaca
      661 aagtggacga cgccgacaga gagaaaaagt ccgaaacatt tccagacatc gagcggactg
      721 acgagcagga tgttgccgac gagcatgttt ttgatttcct cgtcgccttc cgccgcgcgg
      781 ctcaggagaa gcaccgccgc cgccgccgcc accacgtagc tgatgccgat gaccgcctct
      841 tgcggaatct ggctcgtgcg cttgccggtg acggagaaaa tcgcggcgcc gaccagagtg
      901 aagccaagcg cgatgaggaa agccggccag ccttccaggt tcaagtggag caggaccgcg
      961 acgcaaatgc ctaacgaagc catttgcgcc atggcgatat cgatgaagat aattccccgc
     1021 ctaacgacgt ggaggccgag atagaccagc aaccacggca acaggacgca ggccacgacg
     1081 ggccaaaaca tgacatccca cataatcaga tgaccggcgg cgctttcgta tttccgggca
     1141 gcgcacccgc ctcgcgcgcg gcgatcggcg ccccgccgat cgcttccttg gacgcgacgc
     1201 tggatgcgaa gatcatttcg gcggggcgcc gaaatgggca cgcgaggcgc gtgcgctccc
     1261 cggagcagac tggcgctttc atttcagggc cgcggccagg cggttcacga gggcgtcgac
     1321 cagctgcacg taggtgtccg tgttcggtaa cgcgccgggg aattgcgcaa aatcaaccac
     1381 cttcgcgccg gtcgattcgg cgactttctc ggcgattttt cggttgtgat acggctcgac
     1441 gatgatcgct ctgatcttct gcgccttcat ctgcgcgatc acctccgcga gatgcgaggg
     1501 cgagggcggt atccctggtt ttggttccag gaagacgctg atgttaaagc caaaacgatg
     1561 cgcgaaatag acccaggagt cgtgatacgc gaccaggctt tgtccgctga agggcaacaa
     1621 ctttgcgccc cattcctgca gcttggcgtt gatcgtcgcc tcaaactttt tttcgtttgc
     1681 ctggtagtcg gccgcgtgcg cgggatcgac cgcggcgaag gcattcgcga tatgttgcgc
     1741 gaccgccttg gcgatgatcg gatcgaccat gaaatgcgga ttgcccatcg cgtgcacgtc
     1801 gcccgcggcg cgcgttaatt gcgctggcac ctccatcaac cgaatcccct gcgacgcctc
     1861 cacccgcccg ggttggcccg cctcgatctt cggattacgc gcgttctgca ggagcggcgg
     1921 caaccagccg atttcgagtt ccgcgccgcc gtcgatcagc acgtcggcct ggcgcagttt
     1981 gaccacgaaa ctgggccgcg catcgacgaa atgcggatcc tcggttggtt tcgcgagcac
     2041 cgtgacgtcc gcgttgttgc cgccaatctc gcgcgcgatc gagccatagt cggacagcgt
     2101 cgcgacgacg ttgattttgg cctcggccga aaggaggagc ccagcgaaga gcgcaccgcc
     2161 gctgcaaaga agtttgattt ttttcatagg cttagaattt gtgggcgccg tgcgcgccga
     2221 gaatgaactc gaactgaaag aagagggagt cagcctcgcg cgcggcgaga aagtcgtttg
     2281 cctccaggaa atcgtggttg tactgcaaac ggattttcga aaactcggtc ggataccagg
     2341 tcatgtcacc cgagacacgc gagcggctct ggcgagcggg atcgtcggta aaggctgaat
     2401 cctccatgtg cagatagtcg ccgcgcactc cggcggtcca cccttttttg aaaccccaga
     2461 tgatctgcga gtacattccc caatcgtgga aggtttcggc caccgggaat gaatcatcga
     2521 ccccgcggcc ggcttcaaag cggcgataca tcgcctcgct ttgccacttg acgaagggaa
     2581 acccgccctc cgcgcgcggc gatttccatt tgtagagaag gtcggctccg taaatctgcg
     2641 tgcgcgtgtt ggcgccggtg tcgttcgagc cgaaggctcc ggaaacgccc gccaggatgg
     2701 tctgcgtcgg gctcaggtcg accgagtttt cccagcgcgg gacgtaaacg aaatcctgca
     2761 atccgcgcgc ttcccgatcg gtcgtcaggc gaccgtaaaa gacgccattg tcgcccgggt
     2821 tgcggaacga gtagccggtg ttaccgcgcc cgttctgcac tccgaaaaca aactgcgaat
     2881 accaggcgac cggcaccgtc caggagattt gcgcgccgac tccgcgcagc ccgtcggacc
     2941 cgagaaagcg cccatggacg agcgggtcgt cggcgaaatc ccaggtgtgc ggatgggttg
     3001 gattgattcg gccaaaggcg gagaaaaatt gcccgccctt gacctgcaaa ccccatggca
     3061 ggtcggtcgt ctgcatgaac gcctcctcca gctcgatctc cgtttcgttg tcgttgttga
     3121 gtttcaggac gatgttggcg aagccctcga aataagggtc gaccgcaccg tcgagcgcga
     3181 tttccgcgtt gcgcgcattg aagccgcgct gctgcgggtc gtgatcgccg acctcgagtt
     3241 ggtcgaggtc gcgatcgctc gaataggcga gtgagaacac gctgtcgaac gacaggttca
     3301 tgtaggtttt tcctccgccc gcgatcgtga tcggggcggt gagcgaagcg ccggcgccag
     3361 tggaggagat ggtctcgggg gaggtcgtga ccgaggcgtc ggtcgttggg aatgaaccat
     3421 tcacgggaac ggcggctggg ctgtttggtg cgggcggcgc ggacgattgt ggcgccgagg
     3481 cgacgactga ttcgtcggtc gtggggaaga gcggcggcgc gctcgccgcg ggctgcggag
     3541 tcgccgcagg gctttggttg gccgccaacg tgcccggttc cgggttcatt ttcgcgaggg
     3601 tgtcctgctg ctctttcacc tgttgctgca atgccttcac tgtctcggtg agggcctgga
     3661 cttgctggcg caaaacttcg acgtcgttag gcgaaggcgc cggcgacggg ctctgcgcaa
     3721 aaactgccgc ctgcgcgccc agcgccgcca ggacgataaa taactttttc acgcttcatc
     3781 tccattgatc gctccgggct tagccggatg tttctcaatc acgaacgagt gcgtccggcg
     3841 cgttttggac tcagcgcggc gcgggaacgc tcgggcgacg ctgccgcgcg gctggcgcgg
     3901 tcaaatccaa tcgttaggcg agcgccggcg gcgcgtgctc gagacgggaa aattccaggc
     3961 ccgcggcgag cggctggagg tattgcggaa caaaaaccga tgtcgagagc agcgcgaccg
     4021 gagcggcccc gggcgcgcca cccgccagat gatcacaggc gccggataga acgattgtga
     4081 ccgcgcattc gtgatctgct ccgagcaact tgtgaaagtg ttcgtgcagg cggggcgcat
     4141 cggagagcgc gatcgcaaaa agaaacgcgc ccatgattcc caacgcggaa acgcggcgcc
     4201 agggcgagac cgcaccgcga agaaaaccca accgcatgcg aacgagttag ccaccgcggc
     4261 cgcattaggc aagcgggatt tatcctgttt gcccaaaatc ccgcttctta atgaagggca
     4321 aactcgtaga gaagaccgag aacaccgctg ccgacgacga ctggaactac gtcccacctc
     4381 catttcaaca ttccgacgaa agcaacgatc gtgatggtca gtgcgaacca gtcgacgacg
     4441 tgatgtttgg gaaagagcgc attcaaagcg aaccagaccg cgagattcaa gacgactccg
     4501 acaaccgcag cggtgatagc ggaaagtgcg gtggtcagtt tgatgttgcc gcgcaatttc
     4561 tcgatgtaag gcccgcccaa aaaaatccag agaaagcagg gcgtgaacgt gacccaggtg
     4621 gtgagcaatg cgccgaccgt ggccgcgaat aacggcgaca gaccttgcgg atgctgccat
     4681 ccgcccataa aaccaacaaa ttgcagtacc atgataagcg gcccaggtgt cgtctcagcg
     4741 agaccaagcc cgtccatcat ctgccccggt tttagccagc cgtaatgaaa gagggcttgt
     4801 tgcgtgacgt agggcaagac cgcatacgcg ccgccgaagg tgacgacggc tgctttgcta
     4861 aagaaaatgc cttcttggaa aagggtgtga ttccagccta accacaatcc gactgccatg
     4921 accggcgcga gccaaaggac aagccagaca agcgtgactt tgatggcacg ggggaaagaa
     4981 ggctgagtgt gcgccgggga ttccgtttca tcgtcgagga ccgaagtgtt gccggccttg
     5041 gcatgaccgc tgacgacatc aaatttcgat ttccaaaggt agccgccgag aaatccgatc
     5101 aggcccgcgc caaggataat aagcgggaaa ggcactttga agaagaagat cgcgacaaag
     5161 gcgagcgcgg cgagcgtcca catcacttcg ttcctgagcg cttttcgccc gatgcgaatg
     5221 accgcgaccg cgacgattgc catcaccgcg ggtttcagcc cgtagaagat cgcggcaatc
     5281 cacgggaggt ttccataagc ggcataaacg aaacttagtc cccaaagaat aaagattgag
     5341 ggaatgacaa aaaaggcccc ggccactatg ccgccccagg ttttgtgaag cagccagccg
     5401 atgtaagtgg cgagctgctg cgcctcgggg cccggaagga gcatgcagta attgagggcg
     5461 tgcagaaagc gcgcctggct gatccatcgc tttttctcga ccagctcggt ctgcataatc
     5521 gcgatctggc cggtcggccc gccgaacgag atgaagccga gccttaacca aaatcggaac
     5581 gcttccccaa agctcgggtg ttgtctttcg accaccgagc gaccttcact ttgtgaggct
     5641 tcccggttca tcgacgttgc aggaattcgt aaaggccgtc gaagcaagca aagcccgcgg
     5701 tgagaatctc ttgatcggtc atggggcttt ttccccagcc tttgagcacg cgatcgatcc
     5761 cgatacattc cacgcgctga aatttcgcgt cgtccaaatc agcatcgtga atcatttcgc
     5821 cgattttgcg cacggccttg tcgtcgatgt caaagcggcg gattaacgtt tcaaatgtgc
     5881 aaaaggcgtc gtggtgcgaa aactccacgt ccaacatgtc gaatggaata atgccgggat
     5941 gcaacggcgg gtcggacgcg aacacaaact tcgcttccgg gtcgatgaat ttgcaaatca
     6001 gccaggccga gccaacccgg tcaatctccg gccgcggacg ggtctgccag tttttgccgc
     6061 gataatcctt cgaatcgagc cgcggcaacg ttttcctcgg agtctcttca gcccttctca
     6121 agagcatcgc gacctcgtgt ccgcgcggac tatcaaagaa atcaatctcg cgaagctcgc
     6181 gaaactggcg ggttaacctc tccagctccg aaggcgcgct ttccgcgctg gatttctttc
     6241 tcttggtcag gaacgactgc aaagttttct tcagcgcgac atactcctga tcgcgcgccg
     6301 aattaaaaaa agagatcact ttttcctggg tcagactttc gatctcttgt gcacggacga
     6361 gcgtgcaatc accgccgcta tcgcggatct ttttc
//
LOCUS       DATMNS010000231         2754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1055934, whole genome shotgun sequence.
ACCESSION   DATMNS010000231 DATMNS010000000
VERSION     DATMNS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055934"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VNW28_08150"
     CDS             complement(<1..591)
                     /locus_tag="VNW28_08150"
                     /inference="COORDINATES: protein motif:HMM:NF015780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09941.1"
                     /translation="MKTERRAIPAVGKLLEALSVADLPRLLVTQLVRQHLALLRRVGN
                     VPPFETIVRDLEAEIIRLGQTRLQPVINGTGIVLHTNFGRAPLSPAAVQRVAEIAAGY
                     SNLEYDIAGGGRGARGAYLERALALTCGSEAATVVNNGAAALVLIVRHCTKKKREVII
                     SRGELVQIGGGFRVGEMLETSGARLREIGATNKTTLD"
     gene            complement(743..1735)
                     /gene="selD"
                     /locus_tag="VNW28_08155"
     CDS             complement(743..1735)
                     /gene="selD"
                     /locus_tag="VNW28_08155"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002098.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HXA09942.1"
                     /translation="MSQQALAQVLRQLPQYPNRDVLVGSATADDAGVFRLDRDRALVQ
                     TTDFFTPIVDDPFAYGQIAAANALSDIFAMGGRPITALNILGIPIDLVSEKTIAAILR
                     GGLEKAREVGCAIIGGHTIRNPEPIYGLAVTGIVSPKKMLTNAKARPGDRLVLTKPLG
                     TGIATTGIKNGLTSRKLSAAAIQLMSRINTVGAELAERGLVRAATDVTGFGLLGHLAS
                     MCRASGVSAEIDAPEVPAISEEIFELIARGCIPGGTEQNLKTARAFTEWKRTSRRQSI
                     LLTDAQTSGGLLLAVAPRKLDAVLRLLSEHETLCRAVIGKILPSRKPLIRISTS"
     gene            complement(1978..2068)
                     /locus_tag="VNW28_08160"
     tRNA            complement(1978..2068)
                     /locus_tag="VNW28_08160"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2031..2033),aa:Sec,seq:tca)
     gene            complement(2224..2412)
                     /locus_tag="VNW28_08165"
                     /pseudo
     CDS             complement(2224..2412)
                     /locus_tag="VNW28_08165"
                     /inference="COORDINATES: protein motif:HMM:NF021758.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
     gene            complement(2632..2748)
                     /locus_tag="VNW28_08170"
     CDS             complement(2632..2748)
                     /locus_tag="VNW28_08170"
                     /inference="COORDINATES: protein motif:HMM:NF044379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09943.1"
                     /translation="MEYISADEYVEVTPKSLRLRKRVLDATARKRAANAVAA"
BASE COUNT          514 a          805 c          910 g          525 t
ORIGIN      
        1 gtccagcgtc gttttattgg tggcgccgat ttcgcgcagc cgcgccccgc tcgtttccag
       61 catctcgccg acgcggaatc cgccgccgat ctgcaccagc tcaccgcggg aaatgatgac
      121 ctcgcgtttt ttcttcgtgc aatggcgcac gatcaggacg agcgcagccg cgccgttgtt
      181 cacgacggtg gccgcttcgc tgccgcaggt gagggcgaga gcgcgctcga gataagcacc
      241 gcgcgcgccg cggccaccgc ccgcaatgtc gtattccaga ttgctgtagc ccgcggcaat
      301 ttccgcgacg cgttggacgg cggcgggact gagcggcgca cggccgaaat tcgtgtgcag
      361 aacgatgccg gtgccgttga tgaccggttg gaggcgagtc tggccgagac gaatgatctc
      421 ggcttcgaga tcgcgcacga tcgtttcaaa cgggggtaca ttgccgacgc gccggagcaa
      481 ggcgagatgt tgcctaacga gctgggtgac gagcagacgc ggcaaatcgg caacgctaag
      541 tgcctcgagt agtttcccga ccgcggggat ggcgcggcgc tcggttttca tctcgggaag
      601 cggtcgccgc ggcgacccgg tttctgcgac tcgcggaaac catctttggt gatggacggc
      661 gcgctgggaa aagttcgcga cggcggggtc gccgtcgcca gcacgcgagt cgcgtgcgct
      721 cccaggagct gccgcgaccc gctcatgatg tggaaatgcg aatcagtggt ttgcgcgagg
      781 gaagaatttt tccgatgacc gcgcggcaca gtgtctcgtg ttcgcttagc aggcgaagga
      841 cagcgtcgag tttccgcggc gccaccgcca gcagcaaacc gccgctggtt tgcgcgtcgg
      901 tcagcagaat gctttggcgg cggctcgtcc ttttccattc cgtgaacgca cgggccgtct
      961 tgagattttg ctcggtgccg cccggaatac agccgcgcgc gattaactcg aaaatttctt
     1021 cgctgatcgc cgggacctcg ggcgcgtcaa tctccgcgct cacgccgctc gcccggcaca
     1081 tggaggcgag atgaccgagg aggccgaagc cagtgacgtc ggtcgcggcc ctaacgagac
     1141 cacgttcggc cagctccgcg cccaccgtgt tgattcgcga catgagctgg atggcggcgg
     1201 cagaaagttt gcggctcgtc aggccattct tgattccagt ggtggcgatg ccggtgccga
     1261 gcggtttcgt gaggacgagt cggtcgccgg gccgcgcctt cgcgttcgtc agcattttct
     1321 tgggcgagac aatgccggtc acggcgagac cgtaaatcgg ttccgggttc cgaatggtgt
     1381 gtccgccgat gatggcgcat ccaacttcgc gcgctttctc gaggccaccg cgcaggatgg
     1441 cggcgattgt tttttccgaa acgaggtcga tgggaattcc caggatgttc aaagcggtaa
     1501 tcggccggcc gcccattgcg aagatgtcgg agagcgcgtt ggcggcggcg atctggccgt
     1561 aggcgaacgg gtcgtcgacg atcggggtga agaagtcggt tgtttgcaca agggcgcggt
     1621 cgcgatcgag gcggaaaacg ccggcgtcat cggccgtggc ggagccgacg aggacatcgc
     1681 gattcggata ctgcgggagc tgacgcaaaa cttgcgccag ggcctgttgg ctcagcttgg
     1741 aagctcagcc ggcgcaggaa gagagcgagg tcagtttacg gatttgctcg cgcgacatgc
     1801 ggatcacctc ctttcgaacg ggccggaaat ttctcaggaa cgcaggaacg caggaaaagg
     1861 gaaggttgga gaggaccgac caaccccgca cccgtcaagg ccaaacggcg aattaggcaa
     1921 aagccaagcc cctgttttct attcctgctt tcctgctttc ctgagagatc tttggggcgg
     1981 agaggatagg aatcgaacct acctcgatcg gtgaggaccg acggcgattt tgaagatcgc
     2041 gagggccacc aggcaccctt cactctccgg aaaaataaag aatcgctcag gaacgcacga
     2101 acgcaggaag gggaaagctg gcaaggatca accgacactc tcgagtagga ggccaaaccg
     2161 cgaagcctgc aaaaattcag ccccgccgtc tgtttcctgc tttcctgctt tcctgagaga
     2221 tccttagcct tttgctttct tgatctgcct aacgagggcg tcgaaatccg gccattcgcc
     2281 ggagtccagc ttggaatgga gcgttttgcc atgggcactg acttcgaagg cgccgccgct
     2341 cgatgggacg agagtgagcg acgcgatgcg atcgccgagc tcgagaatct tagccgccaa
     2401 actgacggct ttgggcgcgt agtttcaaac gctgcaatac tcgatccgga cttcggtttt
     2461 catcgctccc tcctttcccc tcacgagatt aacgggaagc aaacgcgggt caagacgtag
     2521 ccgcttcgct ctgcgacgcg caggagtggc gtccacttga ggatcggcct cgtgtttccc
     2581 gcggggaaac gtctacaaca agcgaagggg aacgcgtcgg atggccgctc gtcaggcggc
     2641 gacggcgttg gcggcgcgtt tgcgggccgt cgcgtcgagc acccgcttgc gcaggcgcag
     2701 ggacttgggg gtgacctcga cgtactcgtc ggcgctgatg tactcgatcg cccg
//
LOCUS       DATMNS010000232         3553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4469973, whole genome shotgun sequence.
ACCESSION   DATMNS010000232 DATMNS010000000
VERSION     DATMNS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4469973"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..495)
                     /locus_tag="VNW28_08175"
     CDS             complement(<1..495)
                     /locus_tag="VNW28_08175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXA09944.1"
                     /translation="MKKILLSLLQVAVTIAVLIWVFHDPHKRAQMAVALRMADYRWVV
                     AALAAYFLVEFAAAVRWWILLRVQRIHLSMPRVSGLFLIGMFYNQFLPGGTGGDIMKS
                     YLLLKETPGKATGALLAVVFDRMVGLVALISITGALIALRYDFLTRLPETKHLIWLLV
                     AILAS"
     gene            complement(492..1406)
                     /locus_tag="VNW28_08180"
     CDS             complement(492..1406)
                     /locus_tag="VNW28_08180"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXA09945.1"
                     /translation="MLSFSTCWNSSRHTAGDEMLREIKTELGFDLIELGHGIRISLMP
                     GIQEMFEAGEVRFSSLHNFCPLPVEVMGASPDCYTFSNTSPRERQRAIRQTLQTIDFA
                     ALFGAPFVVLHLGRVPIKPVTEPLIALAQAGEMYSRAYVRRKVDAVAKREAAAPAYLE
                     NVKECLKPIMEHAAAKNIRLGIEGRRGYEEIPSEREIIPLLDELDSPQVGYWHDFGHL
                     QIKENLGFVDHEEWLRAIGPRTLGCHVQDCVWPAQDHQPPFAGNVDLEKLVPLLPPTC
                     LFVWEMSPRRTVEEIRHSVTVWKERFGE"
     gene            complement(1422..2702)
                     /locus_tag="VNW28_08185"
     CDS             complement(1422..2702)
                     /locus_tag="VNW28_08185"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXA09946.1"
                     /translation="MKAGAKSWRREALALLHAGPFRRYLIGTSISDTGTWMQVMAQGW
                     VMSTLTDKAIMLGMVNFAAGLPMIFLTMFGGSVADQFDKRRILVWTQVVQIVLALVVG
                     FLIFTGRIQIWHIIAVAGLLGIAFAFEHPALSALVPELVRREEIAGAVALDRAVFHGA
                     RLVGPSVGGVIVAIWGAAMAFFVNAFSFVAFIIALLSLPPRPVGSQEEEAQRASGIKD
                     GFRYIRADKPSLAMVGMIASMTIFIFPTVSVMMPLYVRDVLHFGPNLLGYLMAFSGVG
                     SVCGAITLVSIPPPRRLGGMMAAALAVSVSALGLSRVHAFVFAAASLVVISFGLSLIF
                     GLSNTIVQERAPSHLRGRVSAVMGLSFFGLMPIAGLGVTSLADAIGMRTALVVSAIGY
                     GVAAIFILSRIGRRLNDTPTARTSATVPEVATVS"
     gene            complement(2771..>3553)
                     /locus_tag="VNW28_08190"
     CDS             complement(2771..>3553)
                     /locus_tag="VNW28_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09947.1"
                     /translation="ISVTSETDQATGVAFPVGATLFGVGKLYRDYSGPETGGAPVDQR
                     LFFTHTPGHNNLLLDHRIVPLDMTAPVAEEPRAFEENLENPQALPASGADAQATPIFA
                     TGGAELSNGEKTPWNWWRLNYTGNPSQRSPFWIVRVPKEILNGHGDIFNPNAIDMMAA
                     LFRLSNLLDGKEAPPIQINSKPGAVGQPLAEAVTLAAPGPLPNNKAATAPVPAAGLPA
                     AQASPPTPLAHAAPSTARRPREHKKRPRKSVYLHLVGTAPAK"
BASE COUNT          734 a         1097 c         1147 g          575 t
ORIGIN      
        1 gctggcgaga atcgcgacca gcagccagat gagatgtttt gtctcgggaa gccgggtcag
       61 gaagtcgtag cgcagcgcga tcaaggcgcc cgtgatgcta atgagcgcga ccaacccgac
      121 catgcgatca aagacgaccg cgaggagcgc gccggtcgcc ttgcccggtg tctccttgag
      181 caggagatag ctcttcatga tgtcaccgcc cgtgcccccg ggcagaaact gattataaaa
      241 catcccgatc aggaaaaggc ccgagacgcg cggcatgctg agatggatcc gctgcaccct
      301 gagcaggatc caccaacgca cggcggcggc gaactccacc agaaaatagg cggccaatgc
      361 ggcgacgacc cagcgataat cggccatcct cagcgcgacg gccatctgcg cgcgtttgtg
      421 cgggtcgtga aagacccaga tcagcacagc gatcgtgacc gcaacctgga gaagcgaaag
      481 gagaattttt ttcattcgcc gaagcgctct ttccaaacgg tgacggagtg gcggatttct
      541 tccaccgttc tgcgcgggct catttcccag acaaataagc aggtcggcgg caggagcgga
      601 accagttttt ccaagtcgac gttaccggca aagggaggct gatggtcctg cgccggccag
      661 acgcaatcct ggacgtgaca gcccagcgtg cgcggcccga tcgcgcgcaa ccactcctcg
      721 tgatcgacaa agccgaggtt ttccttgatc tggaggtggc cgaagtcgtg ccaatagccg
      781 acctgcggcg agtccagttc gtccagcagc ggaatgattt cgcgttcgct cgggatttcc
      841 tcgtagcccc ggcgaccttc gatgccgagc cgaatatttt tcgcggccgc gtgctccatg
      901 attggcttca ggcattcttt gacgttctcg agataagccg gcgcggccgc ttcgcgcttc
      961 gccaccgcgt cgactttccg ccggacgtat gcgcgcgaat acatttcgcc cgcttgggcc
     1021 agcgcgatga gcggctcggt cacgggcttg atcggcaccc gaccaagatg gagcacgaca
     1081 aagggcgccc cgaagagcgc cgcgaaatcg atcgtctgca acgtctgccg gatggcgcgc
     1141 tggcgctcgc gcggggaggt gttcgagaaa gtgtagcaat cgggcgacgc ccccatcact
     1201 tccaccggca acgggcagaa attgtgcagg ctgctaaagc gcacctcgcc cgcttcgaac
     1261 atttcttgga tgcccggcat gagcgagatg cggatgccgt gaccgagctc gatgaggtca
     1321 aacccgagtt cggttttgat ctcgcgcagc atttcatcgc cggcggtgtg gcggctggaa
     1381 ttccagcagg tggaaaagga aagcatgcgt ttgcctaacg actaggacac cgtcgccact
     1441 tccgggaccg tcgcgctcgt ccgcgcggtg ggcgtgtcgt tcagccgccg gccgatccgg
     1501 ctgagaatga aaatcgccgc caccccataa ccaatcgccg agacgacgag cgcggtgcgc
     1561 atcccgatcg cgtccgccag gctggtcaca ccgaggccgg cgatcggcat gaggccgaaa
     1621 aagctcaggc ccatcacggc cgagacgcgg ccgcggagat gcgaaggcgc gcgctcctgc
     1681 acaatcgtgt tcgagagacc gaagatgagg gagaggccaa acgaaatcac gacgagcgac
     1741 gcggcggcga aaacgaacgc gtgcacccgg ctgagcccga gcgcggaaac cgagaccgcg
     1801 agcgccgcgg ccatcatccc accgaggcgg cgggggggag gaatcgaaac caaagtgatc
     1861 gcgccgcaga ccgagccgac gcccgagaat gccatcaggt atccgagaag attcggaccg
     1921 aaatggagga catcgcgcac gtaaagcggc atcatgacag agacagtggg gaaaataaaa
     1981 atcgtcatgc tggcgatcat gccgaccatc gcaaggctcg gtttgtcggc acggatatag
     2041 cgaaaaccgt ctttgatccc actggcgcgt tgcgcctcct cctcctggct cccgaccggg
     2101 cgcggcggca gggaaaggag cgcgatgatg aaagcgacaa aggagaacgc gttgacaaaa
     2161 aatgccatcg cggcgcccca gatggcaacg attacgccgc cgaccgaagg accgaccagc
     2221 cgggcgccgt gaaaaaccgc gcgatcgagg gccaccgccc cggcgatttc ctcccgccta
     2281 acgagttccg gaacgagggc ggaaagcgcg ggatgttcga aggcaaaggc aatgcctaac
     2341 aatcccgcga cggcgatgat gtgccagatc tggatgcgcc cggtgaatat gaggaaaccg
     2401 acgaccaggg cgagcacgat ttgcactacc tgcgtccaga ccaggatccg gcgcttgtcg
     2461 aactgatcgg cgaccgaacc gccaaacatg gtgaggaaaa tcatcggcag gccggcggcg
     2521 aaattgacca tcccgagcat gatcgccttg tccgtcagcg tgctcatgac ccagccctgg
     2581 gccatgacct gcatccaggt gccggtgtcg gaaatggagg tgccgatgag atagcggcgg
     2641 aatggccccg cgtggagcaa ggccagggct tcgcgtctcc aggatttggc tccggccttc
     2701 atcggaagca acagatacgc ggtgaagggc ggagccgcaa cgcaccgcca gcaaggcaag
     2761 ccgggccgtc ttatttcgcc ggcgcagtcc cgacgaggtg cagataaacg gacttccggg
     2821 gccgcttttt gtgttcccgc ggccgccgcg cggtcgaggg cgcggcgtgt gcgagcgggg
     2881 tgggtggcga cgcctgagca gccggcaggc cggcggcggg aaccggcgcc gtggccgcct
     2941 tattgttagg caaaggcccc ggcgcggcca gagttacggc ctccgccaac ggctgcccaa
     3001 cggccccggg tttggagttg atctgaatcg gaggcgcctc cttgccatcg agcaggttgc
     3061 tcaagcggaa gagcgccgcc atcatgtcga tcgcgttcgg attgaagata tcgccgtgcc
     3121 cgttgaggat ttccttcggc actcgcacga tccaaaaggg cgagcgctgc gatggattgc
     3181 cggtgtaatt cagccgccac cagttccagg gtgttttctc tccgttgctc agctctgcgc
     3241 cgccggtcgc gaaaattggg gttgcctgcg cgtccgcgcc gctcgccggc agcgcctggg
     3301 gattttccag gttttcctcg aacgcacgcg gctcctcggc gaccggcgcc gtcatgtcca
     3361 gcggcacaat ccggtgatcg agcagcaagt tattatggcc cggagtgtgc gtgaaaaaca
     3421 acctttgatc gaccggcgcg ccgcccgtct cgggacctga gtagtcgcgg tagagcttgc
     3481 ccacgccgaa gagcgtcgcc ccgaccggaa aggccacccc ggtggcctga tcggtctccg
     3541 aggtcaccga gat
//
LOCUS       DATMNS010000233         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4993411, whole genome shotgun sequence.
ACCESSION   DATMNS010000233 DATMNS010000000
VERSION     DATMNS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4993411"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1423)
                     /locus_tag="VNW28_08195"
     CDS             complement(<1..1423)
                     /locus_tag="VNW28_08195"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXA09948.1"
                     /translation="MSKPSCRPGLAGLLMLIGLSSAAATPSPTPATPTPSPAPVLYSA
                     QTLAELKQLQQAALVSDYAYRQVAHLADNIGPRLSGSAQAQKAVEYVAGELKALGLEV
                     QLEKVMVPHWVRGEETAALVQFPGQAPKTTQKIVLTALGGSVATPATGLTAEVVVVRD
                     FEELASLGKEKVNGKIIVFNHAFDRQMAAEGHGGEAYGEAVVYRSDAPSAAARLGAVA
                     ALVRSVGGAAYRLPHTGATHYATDAPKIPAAAVTEEDADLIAALAPQGPVRMHLVLTP
                     QELPDALSYNVIGDLKGTEHPEQVIIVSGHLDSWDLGTGAIDDGAGVAVAMQVPNLVR
                     QLGLHPRRTIRVIAWMNEENGSRGGKTYAQEHAGEIANHFAAIETDLGTDHPLGINLV
                     GRPELKSFLVPVAGVLENSGAGLLAVKDEAGSDIDPLAKLGVPNFSPIQDSRFYFNYH
                     HTAADTLDKIVPRELAETAAINAV"
     gene            1545..>2229
                     /locus_tag="VNW28_08200"
     CDS             1545..>2229
                     /locus_tag="VNW28_08200"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXA09949.1"
                     /translation="MPNDTLVSLDLPGLAKLRSGKVREVFDLGETLLFVATDRLSAFD
                     VILPDPIPDKGAILNQISAFWFERIQLAPNHFVTADFARFPIELQPLREQLAGRSMIV
                     RKTKPLPVECVVRGYLAGSGWKDYQATGSVCGHKLPSGLALGAELPKPIFTPSTKNES
                     GHDLNIDWAQCCAMMGEPAAEKVRAWSLQIYGAGKEHAVACGIIVADTKFEFGLIGDE
                     LLLIDECLTP"
BASE COUNT          391 a          691 c          691 g          456 t
ORIGIN      
        1 gaacagcatt gatggccgcg gtttcggcca gttcgcgcgg cacgatcttg tcgagcgtgt
       61 cggctgcggt gtgatggtag ttgaaataga aacggctatc ctgaatcggg ctgaaattcg
      121 ggacgccgag cttcgccaga ggatcgatgt cggatccggc ttcgtctttc acggcgagca
      181 ggccggctcc ggaattttcc agcactcccg caactggcac gaggaacgat ttcagctccg
      241 gtcggccgac cagattgatc ccaagcggat gatcggtccc caaatcggtt tcgattgccg
      301 caaaatggtt cgcgatctcg ccggcgtgtt cctgggcgta ggtcttgccg ccgcgcgaac
      361 cgttttcttc attcatccag gcgatgacgc ggatcgtccg gcgcgggtgg aggccgagtt
      421 gtctgacgag gttcggcact tgcatcgcga ccgcgacgcc cgcgccatcg tcgatcgcgc
      481 cggtgccgag atcccaggaa tcgaggtggc ccgagacaat gatgacctgc tccggatgtt
      541 cggttccttt caaatcaccg atcacgttgt agctcagcgc atcgggcagt tcttgcggag
      601 tgaggacgag gtgcattcgc accggtcctt gcggcgccag cgccgcgatc aggtcggcgt
      661 cttcttctgt taccgcggcc gcgggaattt tcggcgcgtc cgtcgcataa tgggtcgcgc
      721 ccgtatgtgg gaggcggtac gccgcgccgc cgaccgaacg aacgagtgcc gccaccgcgc
      781 caagtcgggc ggctgcgctc ggggcgtcgc tgcggtaaac gaccgcctcg ccataagctt
      841 cgccgccgtg tccttcggcg gccatttgtc tatcgaaggc gtggttgaaa acgataattt
      901 tgccgttgac cttctctttg cctaacgaag cgagttcctc gaaatcgcgc accacgacga
      961 cctccgcggt gagacccgtc gccggcgtcg ccacgctgcc gccaagggcg gtgaggacaa
     1021 ttttctgagt cgtcttcggc gcctgccccg ggaactggac gagcgctgcc gtctcttcgc
     1081 cgcgcaccca atgcgggacc atcaccttct ccagttggac ctccagaccg agcgccttca
     1141 gttcgccggc gacatactca accgcctttt gcgcctgggc cgagccgctc agccgcgggc
     1201 cgatgttgtc tgcgaggtgc gccacctgcc gataggcgta gtcgctcacg agggcggcct
     1261 gctgcaattg cttcaactcc gcgagcgtct gcgccgagta gagaaccggg gccggagagg
     1321 gcgtcggcgt cgcgggagtc ggagagggcg tcgccgcagc ggacgacaaa ccgatgagca
     1381 taagcaagcc ggcaagcccc gggcggcagg aaggtttgga catgccgcat tctgccgaga
     1441 aaccccgcaa tctcaatctc cttgctggag gcgggtcgaa tgatcgatct ctgattggcg
     1501 acaagcactc gttctcgttt atcttggccc cgacccgcta ttctatgcct aacgacaccc
     1561 tcgtttcact cgatctgccc ggcctggcca aactccgcag cggtaaggtg cgagaagttt
     1621 tcgacctcgg cgagaccttg cttttcgtcg ccaccgaccg cctctccgcg ttcgacgtga
     1681 ttctgcccga cccgatcccg gacaaaggcg cgatcctgaa ccaaatctcc gctttctggt
     1741 tcgagcgtat tcaactcgcg ccaaatcatt ttgtcaccgc cgactttgcc aggtttccga
     1801 tcgagttgca gccgttgcgg gagcaactgg cggggcgctc gatgatcgtg cgtaagacca
     1861 aaccgttgcc ggtcgaatgc gtcgtgcgtg gttatctcgc cggctccggc tggaaggact
     1921 atcaggcgac aggcagcgtc tgcgggcaca aacttccatc tggcctggca ctgggcgcgg
     1981 agttaccgaa gcccattttc acgccttcga ccaagaacga gtccggccac gatctcaaca
     2041 tcgactgggc gcaatgctgc gcgatgatgg gcgagcccgc ggcggagaag gtgcgcgctt
     2101 ggagtttgca aatttacggt gcgggcaaag agcacgccgt tgcctgcggg atcatcgttg
     2161 cggatacgaa gttcgagttt ggtcttatcg gcgatgaact gctcttgatc gacgaatgtt
     2221 taacccctg
//
LOCUS       DATMNS010000234         3216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2486170, whole genome shotgun sequence.
ACCESSION   DATMNS010000234 DATMNS010000000
VERSION     DATMNS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2486170"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..616)
                     /locus_tag="VNW28_08205"
     CDS             complement(173..616)
                     /locus_tag="VNW28_08205"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HXA09950.1"
                     /translation="MKKRVLFICIHNSARSQMAEAWLNEICGEEFEAQSAGLEPGALN
                     PLAVKAMAEVGLDISQKGTQAVFDVWKSGQLFAYVITVCDESSAEKCPIFPGPATRLH
                     WGFPDPSVLTGTPEEKLAAVRVIRDDIRTKIERWCEDVRATANTR"
     gene            complement(635..988)
                     /locus_tag="VNW28_08210"
     CDS             complement(635..988)
                     /locus_tag="VNW28_08210"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXA09951.1"
                     /translation="MKLVQIYQCFCEPTRLRILHLLAQSPLCVCHFQDALREPQVKIS
                     KHLAYLRRRGLVQTKRNGNWIIYSLPATLSSELETNLKCLQNCASSDPVFKKDRQRLA
                     QLQSSCSAPAGIFAK"
     gene            complement(992..1441)
                     /locus_tag="VNW28_08215"
     CDS             complement(992..1441)
                     /locus_tag="VNW28_08215"
                     /inference="COORDINATES: protein motif:HMM:NF040413.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6428 family protein"
                     /protein_id="HXA09952.1"
                     /translation="MKLNSLQSLLRAHPDCLPRFILPHGAQIPAHFHLTEVGHVVKKF
                     VDCGGTFRAREACVLQTYVADDFDHRLNAKRFADILDLGRTVLPNDDLEVEVEWDCCV
                     VSQYPIAAGRVVGDRLEFQLESKHTDCLARQKCGCAPAHTTGTTACC"
     gene            complement(1503..>3216)
                     /locus_tag="VNW28_08220"
     CDS             complement(1503..>3216)
                     /locus_tag="VNW28_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HXA09953.1"
                     /translation="VVTVLVLLGQVLEGRARSRTGSAIRALLDLAPSTARLVRAGEDR
                     DVLLDEIKKGDHLRVRPGEKIPVDGRVIEGRTSIDESMLTGESMPVTKAADDRVTGGT
                     VNQTGSLLIEAERVGRDTVLAQIVEMVGQAQRSRAPIQGLADKVAAWFVPAVIASAVL
                     TFFVWWLVGPEPRFAHAIVNAVAVLIIACPCALGLATPMSVMVGVGRGAQAGVLIKKA
                     EAIERMEKVRTLVVDKTGTLTQGRPAVTEILPNDSISAEELLALAAAVESRSEHPLAA
                     AIVAARNSGVPPDPSRGLPARSQNQPAGRRQDASGGTPELRNFQSVTGGGVNGLVGGR
                     RVLVGKAQFLRDAKVSGVDQLERDAARLQEEGNSVVFVAIDGRAAGLIAIADPIKDST
                     PGAIEALHKLGIKIIMLTGDNESTARRVADQLGIDEVEAGVEPQHKNDRVRHLREGGE
                     VVAMAGDGINDAPALAAADVGIAMGTGTDVAMESAGITLLQGDLRGIEKAIRLSRALM
                     RNIRQNLFFAFVYNSLGIPIAAGILYPWFGILLSPMIAGAAMSLSSVSVITNALRLRH
                     LRLN"
BASE COUNT          570 a         1004 c         1007 g          635 t
ORIGIN      
        1 aggacgatca gcggttggtt gtccggccgc gtccgcagct cctcgacccg ctcttccgtc
       61 gtttcgtctc cgacctggaa ggtcgcagcc gacgcgcgga cgtagtggtt gtagatcgcg
      121 ttgatcgctt ccgcgtcagc gacggtggcg aggcggggtt gtgtcgtttt tgtcaccggg
      181 tattcgccgt tgcgcggacg tcctcgcacc atcgctcgat cttcgtgcga atgtcgtccc
      241 ggatgacgcg gacggcggcg agcttctcct cgggtgttcc agtcaggacg gaaggatcag
      301 gaaaacccca gtgcaggcgc gtcgccggcc cgggaaagat cggacatttt tccgcgctcg
      361 attcatcgca gaccgtgatg acgtaggcga acaactggcc ggacttccag acatcaaaaa
      421 cggcctgggt tcctttttgc gaaatgtcga ggcccacttc cgccatcgct ttgactgcaa
      481 gcgggttgag cgctcccggt tcgagcccgg cgctctgtgc ttcgaattcc tcgccgcaga
      541 tttcattcaa ccacgcttcc gccatctggc tccgggcgct gttgtggatg cagataaaaa
      601 ggacgcgctt tttcatggga ttatggctgg atggttactt tgcgaaaatg ccggccggtg
      661 cagagcaact tgactggagc tgggccaaac gttgccggtc cttcttgaaa accggatcgg
      721 acgacgcgca attctggaga cacttcaaat tcgtttccag ctcggacgaa agcgtggccg
      781 gcagtgaata aatgatccag tttccgttcc gcttcgtttg cacgaggccg cggcggcgga
      841 gataggcgag atgtttggag attttcacct gcggctcgcg cagggcatcc tggaagtgac
      901 agacgcacaa cggcgattgc gcgagcaaat gcagaatgcg cagccgggtc ggctcgcaga
      961 aacactggta gatctggacc agcttcatcc cctaacaaca ggcggtggtg ccggtcgtgt
     1021 gggctggtgc gcagccgcat ttttgccggg cgaggcagtc ggtgtgtttg ctttcgagct
     1081 ggaattcgag tcggtcgccc accacgcggc cagccgcgat cggatattgc gagaccacgc
     1141 agcaatccca ttccacttcc acctcgagat catcgttagg caaaacggtc cgaccgaggt
     1201 cgagaatgtc ggcgaagcgt ttcgcattga gtcgatggtc gaaatcgtcc gccacatacg
     1261 tctgcaggac gcaggcctcg cgcgcgcgga aagtgccgcc gcaatcgaca aattttttca
     1321 cgacgtgtcc gacctcggta agatggaaat gcgccgggat ctgtgcgcca tgcggcagaa
     1381 tgaaacgcgg aaggcaatcg ggatgcgcgc ggaggagaga ctgcaggctg tttaatttca
     1441 tcccggcgag tctatatgct catagaagca tgtagtcaac cgcctcggtt tccgctgagt
     1501 aatcaattca ggcgcaggtg acgcaggcgg agcgcgttcg tgatcacgga aaccgaactc
     1561 aggctcatcg ccgcgccggc gatcatcggg ctgaggagaa ttccgaacca cggatagaga
     1621 atgccggccg caattggtat gcctaacgaa ttgtaaacaa aggcgaagaa aaggttctgc
     1681 cggatgttgc gcatcagcgc gcggctcagt cgaattgctt tctcgattcc gcgcaggtcg
     1741 ccttgcagga gggtgatccc ggcgctctcc atcgccacgt cggtgccggt gcccatcgcg
     1801 attccgacat cggcggccgc cagggccggc gcgtcattga tcccgtcgcc cgccatcgca
     1861 accacctctc cgccctcgcg gagatgacgg acgcgatcgt tcttgtgctg cggttcaacg
     1921 ccggcttcca cttcgtcgat cccgagctga tcggcgacgc ggcgcgcggt gctttcgttg
     1981 tcaccggtca gcatgatgat tttgatcccg agtttgtgga gcgcttcgat cgcacccgga
     2041 gtcgagtcct tgattggatc ggcgatcgcg atgaggccgg cggcgcgacc atcgatcgcg
     2101 acgaagacga cgctgttccc ttcctcctgg agtctggccg cgtcccgctc gagttgatcc
     2161 actccgctta ctttcgcgtc acgcagaaac tgcgccttgc cgacgagcac gcgccggccg
     2221 ccgaccagac cgttcacgcc gccgccggtc accgattgga aattccgtaa ctcaggcgtc
     2281 ccgcctgacg cgtcctgccg gcgtcccgcc ggctgattct gcgaccgggc tggaagcccg
     2341 cgcgacggat caggcgggac gcctgagtta cgggccgcga cgatcgcggc ggcgagcgga
     2401 tgttcgctcc ggctttcaac cgccgccgcc agggccagca actcctccgc cgagattgag
     2461 tcgttaggca agatttccgt cacggccggc cggccttgcg tgagcgtgcc ggtcttgtcg
     2521 acgaccagtg tccgcacttt ttccattcgc tcgatcgctt ccgccttttt gatcaggaca
     2581 ccggcttgcg cgccgcggcc gaccccgacc atgaccgaca tcggtgtcgc aaggccaagc
     2641 gcgcaggggc aggcgatgat cagaaccgcg accgcgttca cgatcgcgtg ggcaaaccgc
     2701 ggctcgggtc cgacaagcca ccagacgaaa aatgtgagga ccgcgctggc gatgaccgcc
     2761 gggacaaacc acgccgcgac tttgtcggcc aggccctgga tcggcgcgcg gctgcgttgg
     2821 gcttgcccga ccatctcgac gatttgcgcg aggacagtgt cgcggccgac gcgctccgct
     2881 tcgatcagca ggctgccggt ttggttgacg gtgccgccag tcacgcggtc atcggccgct
     2941 ttcgtgaccg gcatcgactc gcccgtgagc atcgattcat cgatgctcgt ccggccttcg
     3001 atcacccggc catcaaccgg gattttctcc ccggggcgca cgcggaggtg atcacctttt
     3061 tttatttcgt cgagcagaac gtcgcggtcc tccccggcgc gcacgaggcg cgcggtgctg
     3121 ggcgcgaggt cgagcagcgc gcggatcgcg cttcctgtcc gactgcgggc gcgaccttcc
     3181 agcacctggc ctaacaagac caggaccgtg accacg
//
LOCUS       DATMNS010000235         5291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_691989, whole genome shotgun sequence.
ACCESSION   DATMNS010000235 DATMNS010000000
VERSION     DATMNS010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_691989"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="VNW28_08225"
     CDS             complement(<1..200)
                     /locus_tag="VNW28_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HXA09954.1"
                     /translation="MSAKPDPQRMEKIVSLCKRRGFIFQASEIYGGLNGVWDYGPLGV
                     ELKRNLKDYWWRVMVRERDDVVG"
     gene            complement(294..740)
                     /locus_tag="VNW28_08230"
     CDS             complement(294..740)
                     /locus_tag="VNW28_08230"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXA09955.1"
                     /translation="MSEHKMTLTWKRATPDFEYKTYNRDHTWSFDGGHEMAATAAPVY
                     LGNPKLVDPEEAFVASLSSCHMLTFLAVACKKKFMLDEYVDEAVGHMEKNADGKLAIT
                     RVELHPKLKFSGDHEPTEKDIEEMHHFAHENCFIANSVKTEVTVAQ"
     gene            877..2028
                     /locus_tag="VNW28_08235"
     CDS             877..2028
                     /locus_tag="VNW28_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HXA09956.1"
                     /translation="MTNEDLQALLTAMLGTGEGVSDCLFINGKPPLVERHGALHELPM
                     EEAGATLQPAHIDAMAELLIAGSERLRSDLDTTGSCDTSYGLEAVARFRVNIYRQNGQ
                     IGIVMRKLQSIVPTIESLGLPPIFSEIVKEKNGIIFVTGSTGSGKTTTQAAMLNELNR
                     TQSVHIVTLEDPIEFLHPHGKAVFSQRELGKDFPDFATGLRAAMRQAPKVILIGEIRD
                     RETMEIALTASETGHLVFCTLHTINAGQSINRILGLFTTDEEKQVRERLADTLRFIVS
                     QRLVNKLAGGRLLVTEIMGSSLRTRETLLYGEGENRTFQEIIEAGSTLGWHSFDQSLM
                     RAFEEDLISEETAFLFCTHKNKMRRDLEMAKKMRYPVDEEPSGLKMASL"
     gene            2037..2636
                     /locus_tag="VNW28_08240"
     CDS             2037..2636
                     /locus_tag="VNW28_08240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA09957.1"
                     /translation="MNTSDNDDRSRQELRLAQLEIKVLREQLLKSAQEAQRARDELEE
                     LTREIAPTTPAKSVIVPMTPSHGSPSAPPCNGKPRVLCVEDSEPNFRLIENVLRERPG
                     TDLVWADTGERGLEMAREQAPHLVLLDLDLPDIHGSQVLERLQAQPATAATPVIVISA
                     DATPSQIERMLSAGARNYLTKPFDIRRFLCMFDEIFAGA"
     gene            2698..4350
                     /locus_tag="VNW28_08245"
     CDS             2698..4350
                     /locus_tag="VNW28_08245"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HXA09958.1"
                     /translation="MELRPLAWKVLAAGRGRRILLAVVILALLLGGVLLRRTTWEETT
                     HLRFQRDIVNGFYWGSQTVALGQRLSPGENPDSWKAFARGYVGLYDQVGDDAYEGNYG
                     LDYPPLRLLVMSIWAKEVRAKFPGAEDGTPDYVRPLLHVNLLCEMVTAAGIFLLVRRG
                     LRRASGATDSPLFRRVSPRRRATVCGLLAASVVWLDPSLILDTHAWPQWDVWILPFFL
                     FAALAALKRRWFWCGCLLAAGGMLKGQLLFVAPFFLFWPLWQKRWVRALRLVAGFAFA
                     SALFVLPWLLRNASSWCLVATVAGLTAAVLFRRHSRNAAAWTAGVTAVAAFMVGAVNG
                     GSFGWLTIGFLYGSEHYPYLFISSCYNLPSLLADWGWSLKAPLWSLNLGALHLSLTLQ
                     WALRLFYLLALVLCALGAARHARHREPRLLIALAAPWLLMFALLAQMHERYLLWGAVL
                     SAVALGVSLRLSVLHFLFTAMSAAMITHVLLVDKKLDPTLWVIDLLERVRPYASWLLL
                     GCVAIWVWDTFSSRQPSLRLRPVSTLPDEEVGLPLATAAEKV"
     gene            4452..4526
                     /locus_tag="VNW28_08250"
     tRNA            4452..4526
                     /locus_tag="VNW28_08250"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4484..4486,aa:Val,seq:cac)
     gene            4922..>5291
                     /locus_tag="VNW28_08255"
     CDS             4922..>5291
                     /locus_tag="VNW28_08255"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXA09959.1"
                     /translation="MKSDNLNIRSEPPQGPLSDLERSEFYLREGERLAHMGSWSLRAD
                     GIFDYWSPETFVLFGFDPSEGIPTLPKWLTVLHPDDRDLLAKTIERIFHESVRVDVTY
                     RVDHPKQGKRIMHSTGEPVVE"
BASE COUNT          980 a         1670 c         1540 g         1101 t
ORIGIN      
        1 ccgacgacgt cgtcgcgctc gcggaccatc acgcgccacc agtagtcctt caggttgcgc
       61 ttcaattcca ccccgagcgg gccgtaatcc cagacgccgt tcaggccgcc gtagatttcg
      121 ctcgcttgga aaatgaaacc gcgtcgcttg cacaggctga cgatcttttc catccgctgc
      181 ggatcgggct tagcactcac aggttagacg ctgaatctgg aagccaggaa accaggagtg
      241 ataacaacgg aaaagaatcc tcctggcttc ctggtttccc gatgatttcc gatctattgc
      301 gcgaccgtca cctcggtttt gaccgagttg gcgatgaaac agttttcgtg cgcgaaatgg
      361 tgcatctcct cgatgtcttt ctcggtcggc tcgtggtcgc cggagaattt tagttttggg
      421 tgcaattcga cccgcgtgat ggccagtttg ccgtcggcgt ttttctccat gtggccgacc
      481 gcttcatcca cgtattcgtc gagcatgaat ttcttcttac aggccaccgc aaggaaagtg
      541 agcatgtggc agctggagag cgaggcaaca aacgcttcct cggggtcgac cagcttcggg
      601 ttgcccaggt aaaccggcgc agccgtggcc gccatttcat ggccgccgtc gaagctccag
      661 gtgtgatcgc gattgtaagt tttgtactcg aaatcgggcg tagcgcgctt ccaggtgagg
      721 gtcattttgt gttcggacat gcctctagat atgtgcccga ctgcaattcg caagcggccg
      781 tgattccgtc cttcggtgga acaaatcttg cactaaagaa gccgctggaa cgcctcccgg
      841 gcaggtaaaa actgcccccg ggccagccgg ctagaaatga ccaacgaaga tctgcaagca
      901 ctcctgaccg ccatgctggg aactggcgag ggcgtgtccg actgcctttt tatcaacggg
      961 aaaccgccgc tcgtcgagcg ccacggcgcc ttgcacgaac tccccatgga ggaggcgggc
     1021 gccaccttgc agcccgcgca catcgacgcg atggccgaac tgctcatcgc cgggagcgaa
     1081 cggttgcgct cggacctgga cacgaccggt tcctgcgaca ccagctatgg actcgaagcg
     1141 gtcgcccgct ttcgcgtcaa catttaccgc cagaacggcc agatcggaat cgtcatgcgc
     1201 aagttgcaat cgatcgtgcc aacaatcgag tcgttaggcc tgccgccgat cttcagcgaa
     1261 atcgtgaagg agaagaacgg gatcattttc gtcacgggca gcaccggtag cggcaagacg
     1321 accacccagg cggcgatgtt gaacgagctc aaccggacgc aaagcgtgca catcgtcacg
     1381 ctcgaggatc caatcgagtt tctccacccg catggcaaag cggtcttcag tcaacgtgaa
     1441 ttgggcaagg attttcccga ttttgccacc ggcttgcggg cagcgatgcg gcaggcgccc
     1501 aaagtgatcc taatcggtga aatccgcgac cgggaaacaa tggaaatcgc cctgaccgcc
     1561 tcggaaacgg gccacctcgt cttctgcacg ctgcacacga tcaatgccgg tcagagcatc
     1621 aaccgcatcc tcggcctctt cacgacggac gaggaaaaac aagtgcgcga acggctggcc
     1681 gacacgctgc gtttcatcgt cagtcaacgc ctcgtgaaca agctcgcggg tggccgcctt
     1741 ctcgtcacag agatcatggg cagcagcctg cgcacgcgcg aaacgctcct ctacggcgaa
     1801 ggcgagaacc gcaccttcca ggaaatcatc gaggcgggca gcacgctcgg ctggcacagc
     1861 ttcgatcaat ccctcatgcg cgccttcgag gaggacctga tttccgaaga gaccgctttc
     1921 cttttctgca ctcataaaaa caaaatgcgc cgcgatctcg agatggcgaa aaaaatgcgc
     1981 tacccggtcg acgaagagcc atccggttta aaaatggcct ctctctaagt atcaacatga
     2041 acacctccga taatgacgac cgttcccggc aggaactccg cctcgcccaa ctcgaaatca
     2101 aagttctacg cgagcaattg ctcaagtcgg cccaggaagc gcagcgcgcc cgggacgagt
     2161 tggaggagtt gacgagggaa attgcgccga ccacgcccgc gaagtcggtc atcgtaccga
     2221 tgaccccgtc gcatggatcg ccatccgccc ccccgtgcaa cgggaagccg cgggtcctct
     2281 gtgtcgagga cagcgagcca aacttccgct tgatcgaaaa cgtccttcgg gaacgtcccg
     2341 gcaccgatct cgtctgggcc gataccggcg agcgaggtct cgaaatggct cgcgaacaag
     2401 cgccgcatct cgttctcctc gaccttgatc tgcccgacat ccatggcagt caggttctcg
     2461 agcgcctcca ggcgcagccc gcaacagcag ccacacccgt cattgtgatc agtgccgatg
     2521 ccacgccgag ccaaatcgag cggatgcttt cggccggcgc gcgcaattac ctgaccaagc
     2581 cgttcgatat tcgccgcttc ctttgcatgt tcgacgagat cttcgccggc gcctgagaag
     2641 cgcggcgagc gaactttatt tgccgccgcg ctccgccaag tcgatggtgc gcgaccgatg
     2701 gaattgcgcc cccttgcctg gaaggttctc gccgcaggtc gtgggcggcg aattctgctc
     2761 gcggtggtga ttctcgcgct cctcctcggg ggcgtgctgc tgcgccgcac tacctgggag
     2821 gagacgaccc acctgcgttt ccagcgcgac atcgtcaacg gtttctattg gggcagccag
     2881 acggtggcgt tggggcagcg cttgtcgccc ggcgagaacc ctgattcctg gaaggccttt
     2941 gcgcgcggct acgttgggct atacgaccag gtgggcgacg acgcctacga aggaaattac
     3001 ggactggact atccaccgct gcgcctgctc gtgatgtcga tttgggcgaa ggaggtgcgg
     3061 gcgaaatttc ccggcgccga ggacggcacg ccggattacg tgcgcccgtt gctgcacgtc
     3121 aatctgctct gcgagatggt gaccgcggcc ggtatcttcc ttctcgttag gcgcggattg
     3181 cgacgggcct cgggcgcaac cgactcgcct cttttccggc gtgtctcccc gcgccggcgc
     3241 gccacggttt gcgggctcct cgcggcgagc gtcgtctggt tggatccctc gctcatcctc
     3301 gacacgcatg cctggccgca atgggatgtc tggatcctcc cgtttttcct ttttgccgcc
     3361 ctcgccgccc tgaaacgacg ctggttctgg tgcggttgtc tcctcgctgc cggaggaatg
     3421 ttgaaggggc aactgctctt cgtggcgccg ttttttctct tctggcccct ctggcaaaag
     3481 cgttgggtgc gcgccttgcg tctggtcgct ggcttcgctt tcgcctccgc gttgtttgtc
     3541 ttgccctggc tgttgcgcaa cgccagttcg tggtgcctgg tcgcgaccgt cgccggcctg
     3601 acggcggcgg ttctcttccg gcgacactcc cggaatgcgg ccgcatggac ggccggcgtc
     3661 accgccgtgg ctgccttcat ggtcggagcc gtcaacggcg gcagcttcgg ctggctgacg
     3721 atcggtttcc tctacggcag cgaacattat ccctatctct tcatcagctc ctgttacaac
     3781 ctcccatcgc tcctcgccga ttggggttgg tctctcaagg cgccgctctg gtcgcttaac
     3841 cttggcgctc tccatctctc gttgaccttg caatgggctt tgcgcctttt ctatctcctt
     3901 gccctcgtgc tttgtgcgct cggcgccgcc cgccatgcgc gccaccgcga accgcgcctc
     3961 ttgatcgcgc tggccgcgcc ctggctcctg atgtttgcct tgctcgcgca aatgcacgag
     4021 cgctatctgc tctggggcgc ggtgctgagc gccgtcgcgc tcggcgtgag cttgcgcctg
     4081 agcgtgctcc acttcctatt cacagcgatg agcgcggcca tgatcacgca cgtgctcctg
     4141 gtcgacaaaa agctcgatcc gaccctgtgg gtgatcgatc tgctcgagcg agtgcgcccc
     4201 tacgcatcgt ggctcttgct cgggtgcgtc gcgatctggg tttgggacac gttttccagc
     4261 cgccagccga gcctgcgcct gcgacccgtg tccactttgc ccgacgaaga ggtgggcctt
     4321 ccgctcgcga ccgctgccga gaaagtgtaa gcgagcggtt gtggccggcg acgcaggagc
     4381 aaaaaattgc cgcgtcgctt cctcggcttg actcctcact cctcactcgt cacttatcgt
     4441 ttctgcagat cgcgcgatta gctcagtggt agagcgcttg cttcacacgc aagaagtcgc
     4501 aggttcgaac cctgcatcgc gcaccattct ctggtcgcat aaattttttc aggattgcgc
     4561 ctgcgattgt tcttggcgat cgcagtcgcg cacgcgcgga acgatgctcc acagcctcag
     4621 acagaacttg gccgccacca ccggggctct ccaactacga cgaggctgga ctcgccactc
     4681 gtcgtttcaa tcgcgcggaa cccgctgaca tttcgatcga tccccataaa gtgtacaacc
     4741 tgcctgagtt gatcgacttc gcggagcgga ccaaccccga gacgcgagtc gcctgggacc
     4801 gcgccggcag gcagccgccg cggttggcct tggctttgcc aaagggtagt aactgagcaa
     4861 ttcctcgcgc acgtcgggaa tcggcggtac tctgtcccgt accaatcgac gcgggcgaaa
     4921 agtgaaaagc gacaacctga acattcgatc tgagccgccg caaggaccac tgagcgatct
     4981 cgagcgaagt gaattttacc tgcgtgaagg ggaacgattg gctcacatgg ggagctggtc
     5041 gctgagggcc gatggcatat tcgattactg gtcgccggaa acgttcgttc tcttcggttt
     5101 cgatccatcg gaaggcattc ctacgctccc gaaatggctg accgttctgc atcctgacga
     5161 tcgcgatctc ttagccaaaa cgatcgagag aatctttcac gagagcgttc gcgtcgatgt
     5221 gacgtatcgg gttgaccatc cgaaacaggg aaaacggatc atgcacagta cgggagagcc
     5281 agtcgtcgag g
//
LOCUS       DATMNS010000236        20790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3039660, whole genome shotgun sequence.
ACCESSION   DATMNS010000236 DATMNS010000000
VERSION     DATMNS010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 20790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20790
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3039660"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VNW28_08260"
     CDS             complement(<1..208)
                     /locus_tag="VNW28_08260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09960.1"
                     /translation="MRKLKPLPVVAPGRNLAFGSIMAGTGNDEGEANANRELVDALRR
                     SKLFRDYERVFSEATGLPLALRPLE"
     gene            complement(221..1192)
                     /locus_tag="VNW28_08265"
     CDS             complement(221..1192)
                     /locus_tag="VNW28_08265"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HXA09961.1"
                     /translation="MKRIALLTSGGDAPGMNAAIRAVTRRALYHGAQVMGVRRGYAGL
                     IAGDFVPLGPRDVGRILQLGGTMLGSARCAEFRAAEGITRAVESLGKHSVEGLVVIGG
                     NGSQAGAHALAQLGVPVLGVASTIDNDLCGTDVSIGVDTALNIALEAIDRLKVTALSH
                     QRGFLVETMGRNCGYLGLMAGLAGGADAIVIPEVPRDPESVAAELRSAYARGKSHALV
                     VVAEGAAYNAEKLGAYFAEHRERLQFELRVTKLGHVQRGGAPTAFDRVLATRLGAAAA
                     DHLLAGSQGVLVGMQRSEITVTSLGEIIGRQKPLNLELFPLAYSLAQ"
     gene            1316..1762
                     /locus_tag="VNW28_08270"
     CDS             1316..1762
                     /locus_tag="VNW28_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXA09962.1"
                     /translation="MKRILVPVDFSAPSEQALLCARRLAQFFGGEITLLHVVQKIPVA
                     PFPEVPPYLDYVEEDFEEAEKNLHALAVAEEAATSLKIPTVVRTGLPAHEIVEAAREF
                     DSDLIVIATHGYTGWKHFCIGSTAERVVRAAPCPVFVVREKEHDLN"
     gene            1771..2325
                     /locus_tag="VNW28_08275"
     CDS             1771..2325
                     /locus_tag="VNW28_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXA09963.1"
                     /translation="MDEPLLEPLESRVGLHPFLVGLRPHHIKLLADCAMVKSFDPGEY
                     LFRQGEFANRFYLIEQGQVLLEALDHEGNCVVVEEVGAGKLVGWSWLFPPYIWHFDAR
                     ATEPTTALFFYGTILREYCAKDPSLGFELFKRMSQVMVERLQSARTRLLTSRSSRAAV
                     KDSGRGRRALPVAKTKTRRTNDRA"
     gene            2931..3842
                     /locus_tag="VNW28_08280"
     CDS             2931..3842
                     /locus_tag="VNW28_08280"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXA09964.1"
                     /translation="MIHILDTQHLGRPGIIAATALETNDGIALFDTGADSAFTNISTA
                     MHAAGFAPNDVRHVFLSHIHLDHAGAAWRFAEAGATIHVHPRGARHLIDPARLMESAT
                     RIFGDEMELLWGEMRRIPEEQVHITNDEVAIRAGQFAVRSLATPGHASHHNVYHWDDH
                     LFGGDVAGVRLDGGPPVPPFVPPELQVESWLESIERMRALRATRLYLPHFGLVEGDLG
                     GHFDALAERVQRWAIWFRDELRKGRTESELIADFAQYEARDILESGAAEERVADYERA
                     DPSFMAVTASIRYWQKYHPEAMGIGRD"
     gene            complement(3908..5917)
                     /locus_tag="VNW28_08285"
     CDS             complement(3908..5917)
                     /locus_tag="VNW28_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA09965.1"
                     /translation="MISRHNDDVALPDPSADRAPSRSAALKQFFVELKRRKVYRVAVA
                     YVVVAWVLIQVATQVFPFFEIPNWIVRLVVLALIVGFPVALVLSWAYDITPSGIKRTE
                     DTAPAPVASIPEKSIAVLPFENLSDDRENTFFADGVHDDVLSSLARIADLKVISRTSV
                     QQYRTGARNLREVGLALGVAHILEGTVRRAGNRVRVNVQLINACTDAHIWAESYDREL
                     TDLFAIQSELAERIVGALRANLSPREKAGLQVPPTTSLEAYDIYLRARDLFRWSGIGD
                     PRENGEKALPLLEQALARDPQFALAHYLTSRIHGELFWFGYDKSWERLAKSKAAAETA
                     LRLRPEMGEGHLALAFYHYYSARDYEAAIRELTLAQRALPNEADVASALGMIERRRGR
                     WPESLLHLERARQLDPRNISALWNLSETLTYLRRYAEADRVLEEIASIAPEAHLIPVA
                     RAELSLRERGDTTPLREALGRVPQEFDPGGAITTIAVRLALMERDYDEAERRLGTCTE
                     CKYNDVGLCGFVGALDDYTVPRDWYVGLIARGHGKEDEAARAFAAAKEAVLAEAVESP
                     NDPKVLIMRALIDTMLGRAEEAIATGERAVQLLPISADALDGPLLATNLAAIYAQLGR
                     REQALAALETLVRQTGGPTPGTLRVEPEWDPLRGDPRFQGLLTSG"
     gene            complement(6213..9299)
                     /locus_tag="VNW28_08290"
     CDS             complement(6213..9299)
                     /locus_tag="VNW28_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HXA09966.1"
                     /translation="MIPITEKLLSGAGGWEAMKTARDLVQNGRVSAAAYEPPLLAGEV
                     REGQKRYRAGLRVRATNDLENICTCRESRAWGKICAHSLAVGLAHLASAPPAAPVTTS
                     VAPEAVPAPFVSLGEIDARALALHFILPPNFRAAWAKRQIMVCVEAELEGRRLPLAAL
                     PREDRFGCDEFDLIAVNSLGSDVATVNLLSDEAFLRWLPTLRGHPRLSFGKSAVARVL
                     AEVYRPKINLRGTADGLELAAKLAENESALLAGTDAWLLRDGSFLEIGNNLPPRLTSV
                     WREPLRLGGERADEFLALDLPLWREVADVEIPAGIKLPMVEEAPPAFSLKLEGSLQHL
                     RAILRCSYGDRPAFPPMFEPDRRFAFRDPKSPDRFFLRHLAAESAAVARLAEAGFARD
                     AEAFELRDSGRIVRFFAFEYRALPPTWEISIAPRLEKASKELEPVAPTIEIVRSGEDW
                     FELKYSVATGAGEGIPAAEVQRLLRSGQNQTRLKNGRIAVLNPDALDDFEQVLRDADP
                     RQSQPGVYRLNRIQAAYLTNTADEIGAKLIDAAEALRVGDVPAVAGQLGPLGRQLRDY
                     QLTGVAWLTALAAQNLGGILADEMGLGKTVQTLAFLRLQRGQGPALIVCPTSLLANWQ
                     REVERFTPELKVLLIEGTERAEKIARLPGYDIGLTSYGLLRHDAEFYPDLALGAVVLD
                     EAQHIKNPDTQNAQAAFRLKARQRFVLTGTPMENSVRDLWSLMKFVAPGYLGSRTDFR
                     ERYEKPLSEGRASARPAPVLQRRLARRLRPFLLRRRKVDVARELPEKIEQVVPCELGP
                     TQRATYNALLRGIQQGMGASGENDGATRMKMLTGLLRLRQTCCDLRLLGLPPNEQTDT
                     SAKLDLLDELLEEIIDGGHRVLVFSQFVKMLDLVRERLAASGTEHCYLAGLTKNRQQE
                     VDRFQRDETIPVFLISLKAGGVGLNLAAADTVIHFDPWWNAAVEAQATDRAHRIGQTR
                     VVTAYKLIARDTVEEKILNLQKRKRELASAMLETEEPLMSGLTTNDLAELLGLESK"
     gene            9382..10305
                     /gene="rarD"
                     /locus_tag="VNW28_08295"
     CDS             9382..10305
                     /gene="rarD"
                     /locus_tag="VNW28_08295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HXA09967.1"
                     /translation="MQSTRRANDTSGLVAGIAAFVTWGIVPIYWKLLRFLPASEILAH
                     RFVWTCVFMVLLLSWQRRWPEVRGNLRSRRTALYCAASGIAITINWFVFIWAVNAGRV
                     LETSLGYFMTPLVNVLLGAIFLRERLTRAQLLSVLLAAGAVFYLTFGFGRLPWIALTL
                     CFSFGLYGLFRKISGAAPVPGLFLETTVILPVAIGWLIYLARTHELHFGAATPGLSLL
                     LVSTGIVTGVPLLWFAHAARHLRLTTLGFLQYLAPSCSFFLGVFVYHETFRRAQMLTF
                     GLIWIALAIFTADAVARWRLTRPSALEPVEM"
     gene            10466..10990
                     /locus_tag="VNW28_08300"
     CDS             10466..10990
                     /locus_tag="VNW28_08300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09968.1"
                     /translation="MWSEDDFQYAIENTEVILAPQGKIATFGHTSFRFYLISELMDEV
                     NEVRVRDGRIHAERPQILTADHYSRLLLEGFGKKAQRYIEQLRAHAQKFAVVRYGFQF
                     RKTDVMEKTVRASLASVIEQTKAEVERSDEPLSAVIQGVDDAWEVCLLKFTIDLIERS
                     SGGNLGDFRRRGLM"
     gene            11105..12331
                     /locus_tag="VNW28_08305"
     CDS             11105..12331
                     /locus_tag="VNW28_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09969.1"
                     /translation="MKETRPLFLLDPVSRLTTSIIKAGSRLLPAEVILCCAIWFPNGT
                     QAGESRPKPAVGVTGVIVQSRFGGQIFGYDIDATGTEGVLCEAQTLQGGLVLAAVETF
                     DQSTGRILKVLAKSMDQGSDFITLGVVGNSVGLVEREHVKALFKVIRTFSVINPLDTN
                     RFTATWTPPVGTGHLVEEVSRNQGIPNVAVWAEDITSHFQPFVFSSNVAANTFGPVMT
                     ITDQDFTSGANPGLAYDPVNNRAILGHATLGNPFVPGKIATVNLATGVFTKFTGVGLG
                     DVNGMAYDPVTGTACTTSEIDFSVEFYNIATQTGFAEPLPGAQNQFYSGADVEFDPVN
                     RLFLVAQPNSSTASSGSSIHVYDVSGNLIESINGFSFSNAFNVVAAHIALNPGNRLGF
                     IDGPDAGVTQVQSFTY"
     gene            complement(12428..14353)
                     /locus_tag="VNW28_08310"
     CDS             complement(12428..14353)
                     /locus_tag="VNW28_08310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXA09970.1"
                     /translation="MTNLTRERFRIISIGVVAALAAALVRAVLFRFVGSAIPFLTFFP
                     AVIVAALWGGTLAGVITSMLSLLLASFWMGENPLALDSGKLLNLSFFLLTCVLIVWAV
                     RRAHWARLESVALGAKLAESEARFRYLADNIPQLAWMARPDGHIFWYNRRWFEYTGTE
                     PVEMDGWGWQKVHDPSFLPEVIAKWKHALQTREPWEDTFPLRRRDGVYRWFLSRAMPL
                     RDGDGKVAFWFGSNTDITEQRMHGEERQRLLESERAARSEADRASVLKDEFLATVSHE
                     LRTPLNAILGWAQLLRAGDDNDTMREGLETIERNARAQTRIIEDLLEMSRIISGKTRL
                     DVQPTDLAAVIESAIDALRPAAAARQVRITKVLDPVQAISGDPARLQQVLWNLLANAI
                     KFTPKGGRVSVVLERVNSYVEIVVSDNGEGISPEFLPYVFDRFRQQDPSQTRRHGGLG
                     LGLSIVRNLVELHGGSVRVESEGRGNGSAFTIMLPLTSLRQSDSVRAHPSASQSDGWS
                     VNVPLDGVRVLVVDDERDSLELVRRLLEECKADVLLASSAADGLRLASEQPPDVIVSD
                     IGMPEKDGLEMMRELRARSAQSAKIPAIALTAFARSEDRTRAMLAGYQVHLSKPVEPQ
                     ELIAAVANLAGRTGASR"
     gene            complement(14487..15422)
                     /locus_tag="VNW28_08315"
     CDS             complement(14487..15422)
                     /locus_tag="VNW28_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXA09971.1"
                     /translation="MKTNDIAVERLTTGSKLFAYTRWDAIPLVAALLHCAYFFGLFYL
                     FPRTPLWIMLILGFIYAVSISWNINGISHNFIHNPYFRSPLLNRLFSILESITVGFSQ
                     IFYECIHMQHHRGNADRPDEKGETIDWISIYKHGEEGEAEHPFKYAFFSFFREDPKTV
                     YRELKRKNPREAFWGVVELTLFVTTFVILGILNWRYICFLIPFYYLGHSLSYLNGYYR
                     HYGGNPDEPIAWGVSSYEKLYNWTWFYNGYHAEHHFRPKVHWTRMQAFHDQIAAEQRA
                     AGVRVIKPPHALGFLDADLPRHRKPQEQLGSQSIG"
     gene            complement(15517..16227)
                     /locus_tag="VNW28_08320"
     CDS             complement(15517..16227)
                     /locus_tag="VNW28_08320"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXA09972.1"
                     /translation="MESGAPKTSRPLDRANVAAVIPAYHEAVHVRGVAERARAQLDRV
                     LVIDDGSGDATAARAREGGAEVIVHPENRGKGEAIKTGLRHWLAQAEIEFILLFDADG
                     QHLPEEISRFFAAVAASEASLFIGTRMHDVRHMPFARRTVNRYMSRRISRLCGQEVPD
                     TQCGYRMIHRTLAPHLLAGTSRFDYETEMLIIASRAGFRIASVPISTIYSDEVSSIHP
                     VRDSIRFFKLMRRYEKTA"
     gene            complement(16230..17117)
                     /gene="lipA"
                     /locus_tag="VNW28_08325"
     CDS             complement(16230..17117)
                     /gene="lipA"
                     /locus_tag="VNW28_08325"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXA09973.1"
                     /translation="MSCAKIDPALHQARKPPWIKVRLPSNPVFFSTKALISDLRLHTV
                     CESAQCPNRWECWSQGTATMMIAGERCTRACGFCAVTTAKPFALEEDEPQRVAEAVRR
                     LKLKHVVITAVARDDLKDGGAEHFARTIRAIRAMDPSIIIEVLVPDFHAQEWCIQLVL
                     DAGPEIFNHNLETVERLTPAVRSRAKYRTSLEVLRRAKELAPDIVTKSGVMLGLGETE
                     PELFQAMDDLREVGCQVLTLGQYLRPTPQHLPVVGYITPEQFDLYGDIARGKGFAHVA
                     SGPLVRSSYHAADFHPVVH"
     gene            17299..17868
                     /locus_tag="VNW28_08330"
     CDS             17299..17868
                     /locus_tag="VNW28_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09974.1"
                     /translation="MEGQDNEMTHGQENGAIDESGKVDRIRDILFGSQMRDYDGRFQK
                     LEERLTREADELRGDLQRQLEALEGFMKGEAESMTNRVKTEQSERGQACERLAREMAE
                     TARALELKIGNLDSQAAKDIRELREQLLEQSKALSAQLKEKHDHLKAQLDHEAGQIRD
                     AMTGREALAEMLTEVALRLKKEFRVPGTS"
     gene            17872..19986
                     /locus_tag="VNW28_08335"
     CDS             17872..19986
                     /locus_tag="VNW28_08335"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXA09975.1"
                     /translation="MHPRMRSAQPRKPVDDLAELRRLINQPEQDKLRDLRDRLDDKEQ
                     RAHEVASILPEAVTLSGDRSEELTRALRPAVEGSVRESIEKRPEVFIDALHPIIGSIV
                     RRSIAESLRTLLQSLNQALEQSFSWQGLKWRFEAFRTGRSFAEVVMLRSLIYRVEQIF
                     LIHRETSLSLLHLSADPAVAQDSDMVAGMLSAIQDFSHDSFQTGKDEALEEFRIGELQ
                     VWIAPGAHAYLAAAIRGNPPRELRASLEDAIERVHVLRGSALAKFNGDAAQFESLRSE
                     LEPCLRAQYQPQKTAGRTNRAWFALAAGAGLVVAALIAATLSEMRWRDFVRRLNSAPG
                     IAVTQAEKGWISKSRVAFLRDPEVADPAALARAAGINPSRIYFEWKDYLALDDASVRR
                     RFERRFGKPAGTKLELTNGALQISGTVPYEWLARVQQDGVQSPGVTSITDKDVKVTYD
                     PDLALGRFQRAFPPPAGVTAKVENGTLLLAGTAPYEWLAPVRENAVKLPGINRISEEH
                     LQVAYDPKLVLQRFSDRFSLPDTVNATMKDGILVLSGEAAHAWLLRVRPGAPTVPGIK
                     TIDERSLTDLDVRAFQQSKSVIESAFVYFLLNKDNFATEGFAALSRLPDEIRRCLTAA
                     NRLGLEIQIEVRGSADAVGSGKRNAELSQRRAEAVRDFLVNCGFASSLFKTMGLGAPA
                     TAGQPAPEQADRRTSFKIVPKS"
     gene            19983..20483
                     /locus_tag="VNW28_08340"
     CDS             19983..20483
                     /locus_tag="VNW28_08340"
                     /inference="COORDINATES: protein motif:HMM:NF012300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rab family GTPase"
                     /protein_id="HXA09976.1"
                     /translation="MIQKKVCLLGTSGVGKTSLVAQFVHSMFSDKYLTTVGVKIDKKT
                     LPVDDTEVMLVIWDLAGDDDFQRLQTSYLRGTSGYLLVADGTRRVTLDQALELQKRVA
                     ETLGATPFVLALNKADLAPQWEVDEAQISALAAQNFTIIKTSAKEGAGVEESFAHLAR
                     RMMAPA"
     gene            20489..>20790
                     /locus_tag="VNW28_08345"
     CDS             20489..>20790
                     /locus_tag="VNW28_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09977.1"
                     /translation="MPDAESPAQSVLEALGFVLFARESPGTLRLLGKAPAWLGALWPS
                     LRESGGTLPVADASPFLENFLVDAEECWGKGGGERAKSGPWVENGSGAEVELEATAL"
BASE COUNT         4001 a         6422 c         6497 g         3870 t
ORIGIN      
        1 attccagcgg gcgcaaggcg agcgggaggc cggtcgcttc gctaaagacg cgttcatagt
       61 cgcgaaacaa tttcgagcgc cgcaacgcat cgaccaactc gcggttggcg tttgcttccc
      121 cttcgtcgtt gccagtgcca gccatgatgg aaccgaaagc aaggttcctc cccggcgcaa
      181 ctacgggcaa gggctttagc tttctcatcg gccgaaattc ttactgcgcg agactatagg
      241 cgagcgggaa gagttcgaga ttgagcggct tttgccggcc gataatctcg cctaacgacg
      301 tgaccgtgat ctcggaacgc tgcatgccga cgagcacacc ctgcgagcca gcgagcagat
      361 gatcggcggc cgccgcgccg agacgggtgg ccagaacacg atcgaaggcg gtcggggcgc
      421 cgccgcgctg gacgtgcccg agcttcgtca cccgcaactc gaactgcagc cgctcgcggt
      481 gctcggcaaa ataggcgccc agcttttcgg cgttataggc ggcgccttcc gcgaccacca
      541 ccaacgcgtg cgatttgccg cgcgcgtagg cgctgcgcaa ctcggcggcg accgattccg
      601 gatcgcgcgg cacctcggga atcacaatcg catccgcgcc gccggccagg cccgccatga
      661 gaccaagata gccgcagttg cgccccatcg tttccaccag gaaaccgcgc tgatgcgaca
      721 acgccgtcac ctttaagcgg tcgatcgcct cgagcgcgat attgagcgcg gtgtcgacgc
      781 cgatcgagac gtccgtcccg caaagatcgt tgtcgatggt cgaagccacg ccaagcacgg
      841 gaacgccgag ctgcgcgagg gcgtgcgcgc cggcctgcga gccgttaccg ccgatcacga
      901 ccaggccttc gacgctgtgt ttgcctaacg actcaaccgc ccgggtgatt ccttccgcgg
      961 cgcgaaactc cgcgcagcgc gcgctcccca gcatcgtccc gccaagctgc aggatgcgcc
     1021 ccacatcgcg cggcccgagc gggacgaagt cgcccgcgat caatccggcg tagccgcggc
     1081 gcacgcccat gacctgcgcg ccgtgataaa gcgcgcgccg tgttacggcg cggatcgcgg
     1141 cgttcatccc gggtgcgtcg ccgccgctgg tgagaagcgc gatgcgtttc ataagcgcgc
     1201 caaaaaacga ccggactttc gcaaggcatg cgtagcaaag cggcggatcg acttcatagt
     1261 ctgagacgtc atggaaccag gcactttacg caggcgagcg ccggccgcga ttcacttgaa
     1321 aaggattctg gtgccggtcg acttctctgc cccttcagaa caggcgctgc tttgcgcgcg
     1381 ccgcctcgcg caattcttcg ggggcgagat cacgctcctc cacgtcgtcc agaaaatacc
     1441 agtcgcgcct ttccccgagg tgccgcctta tctcgattac gtcgaagagg atttcgagga
     1501 ggcggaaaaa aatctgcacg cgctcgccgt cgcggaggag gccgccactt cccttaaaat
     1561 cccgacggtg gttcgaaccg gcctcccggc acacgagatc gtcgaggcgg cccgggaatt
     1621 cgattccgac ctgatcgtga tcgcgaccca cggttatacc ggctggaaac acttttgcat
     1681 cgggagcacg gccgagcgcg tggtgcgggc ggcgccctgc ccggtttttg ttgtgcgcga
     1741 aaaggagcac gacttaaatt gaacgcgctt atggatgagc ccctcctcga gccgctcgag
     1801 agccgggttg gattgcaccc ttttctcgtt ggcctgcggc cgcatcacat caagctgctg
     1861 gccgattgcg cgatggtgaa gagcttcgat cccggcgaat atcttttccg ccagggtgaa
     1921 tttgccaacc gcttctattt gatcgagcaa ggccaggtcc tgctcgaggc gctcgaccac
     1981 gagggcaact gcgtcgtcgt cgaagaggtc ggcgcaggca agctggtcgg ctggtcgtgg
     2041 ctcttcccgc cctacatctg gcatttcgac gcgcgcgcga ccgaacccac caccgcgctc
     2101 tttttctacg gcacgatcct gcgcgaatat tgcgcgaagg atccgtcgtt aggcttcgag
     2161 cttttcaagc gcatgagcca ggtaatggtg gagcgcttgc aatccgcccg cacgcggctc
     2221 ctcaccagcc gctccagccg ggccgcggtc aaagactccg gcagaggtcg ccgcgccctg
     2281 ccggtggcca agaccaaaac gagacgaact aacgatcgcg cgtgagaatc cgcgcgctca
     2341 ctggcggact gcatttctgc tcgtcgcttc tcgtcatcct ggagggtttg cctaacgacc
     2401 taaaggtctc gcgcgatctg attgtcgcgt agtttctgag ccggagcaca gaaggaccga
     2461 tcctggagca gagcaaatac cacatgctct ctccgaggga ttgagcgccc gcgcccgcaa
     2521 cattccgtcc aaatgtcaaa tgtatacgac cccgtccgcc acgcacagag gagctgtagc
     2581 cgcttcgctg tgcgaagcgc gggtatgtgt gtggcaaggc ggttgtcgcg tcgtaccgcg
     2641 tcgcccacag ggcgacggct acaggaggtg gattttgctg gccgcgctaa cccgcaagtt
     2701 catgacagcc tctcgaggac gtaaagtcag cgcagcgagc cgctttggtt tggcggagag
     2761 caaactttac ggggatactt cagtcgttag attgtgggcc tgaaggcgag cgatctggtc
     2821 accatttccg cccgtgcggc ctaacgaaaa gcaaaaaaaa catcagcgat cacattcggc
     2881 tgcgcctcag tgaaggctct tcgctccgct caggatgaca gtcgttccga atgattcaca
     2941 ttctcgacac ccaacatctc gggcggcccg gcatcatcgc cgcgacggcg ctcgagacga
     3001 acgatgggat cgcgcttttc gacaccgggg cggactccgc tttcacgaat atctccaccg
     3061 cgatgcacgc agcgggattt gcgcctaacg acgttcgcca cgtcttcctc agccatattc
     3121 atctcgatca cgcgggcgcg gcgtggcgtt tcgccgaggc gggcgccacg attcatgtcc
     3181 atccgcgagg cgcgcgacac ctgattgatc cagcgcggtt gatggaatcg gcgacgcgca
     3241 tcttcggcga tgaaatggag ctactctggg gagaaatgcg ccggatcccc gaggagcaag
     3301 tgcatatcac caatgacgag gtcgcgatcc gggccggtca atttgccgtg cgctcgctcg
     3361 ccactcccgg gcacgcgtcg caccataatg tctatcattg ggacgaccac ctctttggcg
     3421 gcgatgtcgc cggggtgcgc ctcgacggcg ggccgcccgt gccgccattt gttccgcccg
     3481 aactacaggt ggagtcgtgg ctcgaatcga tcgagcggat gcgtgcgctc agggcgacgc
     3541 gtctttacct gccgcatttc ggtttggtcg agggcgacct gggcgggcac ttcgacgcgc
     3601 tcgccgaacg ggtacaacgc tgggcgatct ggtttcgcga tgaactgcgt aaagggcgga
     3661 ctgaatccga gctgatcgcg gatttcgcgc aatacgaagc gcgtgacatt ctcgaatcgg
     3721 gcgcggcgga ggaacgggtg gcggattacg aacgcgcgga cccaagtttc atggccgtga
     3781 cggcttccat tcgctattgg cagaagtatc atccggaagc gatgggaatt ggacgggatt
     3841 aacaggattg aactggatta agacaagaaa aaatccgaat gatcccgtct cgatttccca
     3901 agcctgatta gccggacgtc aggagtccct gaaaccgtgg atcgccccgc aatggatccc
     3961 attccggctc gacgcgtagc gtaccggggg tcggaccacc cgtttgccta acgagcgttt
     4021 ccagcgcagc cagagcttgc tcacgccgcc cgagctgcgc gtagatcgcc gccaggttgg
     4081 tcgcgaggag cgggccatcg agcgcatccg cggaaatcgg caacagttgc acggcgcgct
     4141 cgccggtggc gatcgcctcc tccgcccggc cgagcatggt gtcgatcaac gcccgcatga
     4201 tgagcacctt cgggtcgtta ggcgattcga ccgcttcggc cagcaccgcc tctttggcgg
     4261 cagcgaaggc gcgcgccgcc tcgtcttctt tcccgtgacc gcgagcgatc aggccgacat
     4321 accagtctcg cggcacggta taatcatcga gcgcaccgac gaatccgcac aagccgacat
     4381 cgttatactt gcactcggtg caggtgccga ggcgccgctc ggcctcgtcg taatcgcgtt
     4441 ccatcaaggc gagccgcacc gcgatcgtcg tgatcgcgcc acccggatcg aactcctgcg
     4501 gaacccgccc caaggcctca cgcaacggtg tcgtgtcacc acgctcacgc agggaaagtt
     4561 ccgctcgcgc gacgggaatc aggtgcgctt ccggcgcgat cgacgcgatt tcttccagca
     4621 cgcggtcagc ctccgcgtag cgccgcaaat aagtgagcgt ctcggagaga ttccagagcg
     4681 cggagatatt ccgcggatcg agttgccggg cccgttcgag atggaggagc gactccggcc
     4741 agcgcccccg ccggcgctcg atcatgccga gggcgctggc cacgtcggct tcgttaggca
     4801 aagcgcgttg cgcgagcgtc agctcgcgaa tcgccgcctc gtaatcacgc gccgaatagt
     4861 aatgataaaa agcaagggcg aggtgtccct cgcccatctc cgggcgcaga cgcagggcgg
     4921 tctcggcggc ggctttcgat ttcgccagac gctcccaact cttatcgtag ccaaaccaga
     4981 agagctcgcc gtgaatccgc gaggtgagat aatgggcgag ggcaaactgc ggatcgcgcg
     5041 ccaacgcctg ctcgagtaag ggcagcgctt tctcgccgtt ctcgcgcgga tcgcctatcc
     5101 cgctccagcg aaagagatcg cgcgcccgga gatagatgtc gtaggcctcg agactcgtgg
     5161 tgggcggcac ctgcaaaccc gccttctccc gcggcgagag atttgcccgg agcgccccga
     5221 cgatgcgctc ggcaagctca ctttggatgg caaagagatc cgtcagttcc cgatcgtaac
     5281 tctccgccca aatatgcgcg tcggtgcagg cattgatgag ttgcacgttg acccgcaccc
     5341 ggttgcccgc ccgccgcacc gtgccttcga gaatatgggc gacgccgagc gcgagtccga
     5401 cctcgcgcag gttgcgcgcg ccggtccggt attgttgcac cgaggtccgg ctgatgactt
     5461 tcaggtcggc gatgcgcgct agactggaaa gaacgtcatc gtgcacgccg tcggcgaaaa
     5521 aggtgttttc gcggtcgtcg ctcaggtttt caaacgggag gaccgcgatg cttttctccg
     5581 gaatcgaagc gaccggcgcg ggcgcggtgt cctcggttcg tttgatgccc gagggcgtga
     5641 tgtcataggc ccaggacaaa accaaagcga ccgggaagcc gacgatgagc gcgaggacga
     5701 ccagcctaac gatccagttt ggaatttcaa aaaaaggaaa gacctgggtg gcgacctgga
     5761 tcaggaccca ggcgacgacg acgtaggcca cggcgacgcg ataaaccttt cgtcgtttca
     5821 gctcgacgaa gaattgtttc aaagccgcgc tccgggaggg ggcgcggtcg gcgctcgggt
     5881 cgggaagggc cacgtcatcg ttatggcgcg agatcatgcc agcggcaagg ccgtgcggtg
     5941 gtggcgcatt ccgtagcgca agcttcctct cgcggattcc agccagtaag ctggcaattg
     6001 gggagaacgg gcgcacctaa ggtctgggga gcgcaggcgc cctcgcgtgc tggttgcggc
     6061 gcctcgccgc aaccggcttt gccggacacg cgcaaagaca ggcgaaagtt cgcgacggcg
     6121 agggcgccat cgccggcacg cgaggcgcgt gcgctccccg gaaaatagcg cgcgacgctc
     6181 ggcgtgagaa gttagctcac gctctgcgcg gctcatttcg actcaaggcc cagcaattcg
     6241 gcgaggtcgt tcgtcgtcag gccgctcatg agcggttcct cggtctcgag catcgcgctc
     6301 gccagttcgc gttttcgttt ttgcaggttg agaatttttt cctcgaccgt gtcgcgggcg
     6361 atcagcttgt aggcggtcac gacccgcgtc tggccaatcc gatgggcacg atcggtcgcc
     6421 tgcgcttcga ccgccgcgtt ccaccagggg tcgaaatgga tgacggtgtc ggccgccgcg
     6481 aggtttaagc cgacgccgcc ggctttcagg ctgatgagga agaccgggat cgtttcatcc
     6541 ctttgaaaac ggtcgacttc ctgctgccga tttttcgtta ggccagcgag ataacaatgc
     6601 tcggtgccgc tcgccgcgag ccgttcccga acgagatcga gcattttgac aaactgactg
     6661 aaaacgagga cgcgatgtcc gccatcaatg atttcctcga gcagctcgtc gaggagatcg
     6721 agtttggctg aagtgtcggt ctgttcgttg gggggaaggc cgagaaggcg cagatcgcag
     6781 cacgtctgac gcaagcgcag cagcccggtc agcattttca tccgcgtcgc gccgtcgttt
     6841 tctccgctgg cccccatccc ctgctggatc ccgcgcagga gcgcgttgta ggtcgcccgc
     6901 tgggtcggcc caagctcgca cgggacgact tgctcgatct tctccgggag ctcgcgcgcg
     6961 acgtcgactt tgcgccggcg gaggagaaac ggtcgcaagc gacgagccag acggcgctgc
     7021 aggactgggg ctggacgtgc ggaagcgcgt ccttccgaca gcggtttctc gtaacgctcg
     7081 cggaaatcag tccggcttcc cagataaccg ggagcgacaa atttcatcag cgaccaaaga
     7141 tctcggacgg agttttccat cggggtgcca gtaaggacga aacgctggcg ggccttgaga
     7201 cggaaagccg cctgagcgtt ttgcgtgtcg gggttcttga tgtgctgcgc ttcgtcgagc
     7261 acgacggcgc cgagcgccag gtccgggtag aattcagcat cgtgccggag caggccgtaa
     7321 ctcgttaggc caatgtcgta gcccgggagg cgcgcgattt tttcggcgcg ctccgtccct
     7381 tcgatcagca gcactttcaa ttccggagtg aatcgctcca cctcgcgctg ccagttggcg
     7441 aggagcgaag tcgggcaaac gatcagggcc ggaccttggc cgcgctgcag gcgcagaaag
     7501 gcgagggtct gcacggtctt gccgagaccc atctcgtccg ccaggatacc gcccagattt
     7561 tgcgcggcga gcgccgtgag ccaggcgacg ccggtcagtt gatagtcgcg caattgccgg
     7621 cccagcggcc cgagttgccc agccacggca ggaacgtcgc ccacgcgcaa agcctcggcc
     7681 gcgtcgatta atttcgctcc gatttcatcc gctgtattcg ttaggtaagc ggcctggatc
     7741 ctattcagcc ggtaaacgcc cggctggctc tggcgcggat cggcatcgcg cagcacttgt
     7801 tcgaagtcgt cgagcgcatc cggattcagg accgcgatcc tcccgttctt cagccgcgtt
     7861 tggttttggc cggaacggag gagccgttgc acctcggccg ccggaatccc ctcacccgcc
     7921 ccggtcgcga ccgaatattt cagctcgaac caatcctcgc cggagcgcac gatctcgatc
     7981 gtcggcgcca cgggttccag ctccttgctc gccttttcca ggcgcggcgc gatggagatc
     8041 tcccaggtcg gcggcagagc ccgatattcg aaggcaaaga agcgcacgat gcgtccggaa
     8101 tcgcgcagct cgaacgcctc cgcgtcgcgc gcaaagcccg cctccgcgag gcgggcgacc
     8161 gccgcgctct cggcggcaag atggcgcagg aagaagcgat cggggctctt cggatcgcga
     8221 aaggcgaagc gcctgtccgg ttcaaacatt ggcggaaaag cgggacgatc gccatagctg
     8281 caacgcagga tcgcgcgcag gtgttgcagc gaaccttcca gcttcaggct gaaagccggc
     8341 ggcgcctcct cgaccatggg aagcttaatg cccgcgggaa tttccacgtc cgccacttcg
     8401 cgccagagcg gaagatcgag agcgaggaac tcatccgccc gctctccgcc gagtcgcaat
     8461 ggctcgcgcc agacactcgt taggcgcggc ggcagattgt ttccaatctc gagaaaactg
     8521 ccgtcgcgca ggagccaggc gtcagtcccg gcgagcaacg cgctttcgtt ctccgccaat
     8581 ttcgccgcca gctcgaggcc gtcggcggtc ccgcgcagat tgattttcgg ccgataaact
     8641 tccgccagaa cgcgggccac cgccgacttg ccgaagctga gtcgcgggtg cccgcgcaag
     8701 gttgggagcc agcggagaaa cgcctcgtcc gagagcagat tgaccgtggc gacgtccgag
     8761 cctaacgaat taacggcgat caaatcaaat tcatcgcagc caaaacggtc ttccctgggc
     8821 aaagcagcga gcggcagccg gcgtccttcg agctccgcct cgacgcaaac catgatctgg
     8881 cgcttcgccc aggcggcgcg aaaattcgga ggcagaatga agtgcaacgc gagcgcgcga
     8941 gcgtcgattt cgcctaacga aacaaagggg gcgggcaccg cctccggcgc gacgctcgtg
     9001 gtcaccggcg cggccggagg ggccgaagcg agatgagcca gcccgaccgc aagggaatgc
     9061 gcgcagatct ttccccacgc gcgggattcg cgacaggtgc agatgttctc gagatcgttg
     9121 gtcgcgcgca cgcgcagacc cgcgcgatag cgtttttgtc cctcgcgcac ttcacccgcg
     9181 agaagcggcg gctcgtaggc cgcggccgag acccgcccgt tttgcacgag atcgcgcgcg
     9241 gtcttcatcg cttcccagcc gccggcgccg gaaagcaatt tctcggtgat gggaatcacc
     9301 gcgcagcgtc cacgctccgc tcgatcgacg caatgacggc tcgtttgctc gtctctcaat
     9361 tccgtgaaat agaagcgcgc gatgcaatcg acccgccggg ctaacgacac ctcggggctg
     9421 gtggcgggga tcgcggcttt cgtcacctgg gggatcgtgc cgatttactg gaagctgctg
     9481 cgctttctcc cggcgagtga aattctcgcg catcgttttg tctggacgtg cgttttcatg
     9541 gtcctgctcc tgagctggca acgccgctgg ccggaggtgc gcggcaacct gcgttcgcgc
     9601 cggaccgcgc tctattgcgc ggccagcggg attgcgatca cgatcaactg gttcgttttt
     9661 atctgggcgg tgaatgcggg gcgcgtgctg gagacgagcc ttggttattt catgactccg
     9721 ctcgtgaacg tcctcctggg cgccatcttt ttgcgcgagc gactgacccg cgcccagctt
     9781 ctttccgtgc tgctcgccgc cggcgcggtg ttttacctca ctttcggctt cgggcggctg
     9841 ccttggatcg cgctcaccct ttgtttttcg tttgggctct atgggctgtt tcgcaaaatc
     9901 tcgggcgcgg cgcccgtgcc cggacttttc ctggaaacaa ccgtcattct gccagtggcg
     9961 atcggctggt tgatctacct cgcccgcact cacgagctgc atttcggggc agcaacccca
    10021 ggtctttcgc ttttactggt gagcaccggc atcgtgaccg gggtgccctt gctgtggttc
    10081 gcgcatgccg cgcggcatct tcgcctaacg acactaggtt tcctgcaata tctcgcgccg
    10141 agctgcagct tttttctcgg cgttttcgtt taccacgaga ccttccggcg agcgcagatg
    10201 ctaacctttg gactgatctg gatcgcgctc gccatcttca ccgccgatgc cgtggcgcgc
    10261 tggcgcctga ccagaccgag cgcgctcgag ccggtcgaga tgtagccgct tcgctgcgcg
    10321 aagcgcggga gcaagcctgg tcgagagaaa ggcgcaggac aaatcacatc gagagccgcg
    10381 cctcccacag ggaggcggct acaggggcgt cacaggggcg ccccggagac gcgaggacgc
    10441 tgttttgtgg tatggtcgcg ccgcgatgtg gagcgaagac gactttcaat acgcgatcga
    10501 gaacacggaa gtgatcctcg cgccgcaggg gaagatcgcg accttcggcc acaccagctt
    10561 ccggttttat ctgatcagcg agctgatgga cgaggtgaac gaagtgcggg tgcgcgatgg
    10621 gcggattcac gcggagcgtc cacagatcct cacggcggac cattactccc ggctcctgct
    10681 cgagggtttc ggcaaaaagg cccaacgcta catcgagcaa ctgcgcgcgc acgcgcagaa
    10741 gttcgcggtg gtgcgctacg ggttccaatt ccgtaaaacc gacgtcatgg aaaagacggt
    10801 gcgcgcttcg ctcgcctccg tcattgagca aacgaaggcg gaggtggagc gatcggacga
    10861 accactcagc gccgtgattc aaggcgtcga cgacgcgtgg gaagtctgcc tgctcaagtt
    10921 cacaatcgat ctcatcgaac gctcgtccgg cggcaatctc ggcgattttc gtcgccgtgg
    10981 attgatgtaa aggccgcaac ttatttctgg ccgccaacgg ctgccgcttc gctacccggc
    11041 cgagtaactg tttcgttacg tttggccgat tgaggatcgt ggttcgaaga aggacgatct
    11101 cgtcatgaaa gaaacacggc cgctgttttt gcttgatccc gtttcacgtc tcaccacatc
    11161 catcatcaaa gctgggtcca gactgctgcc ggccgaagtc atcctttgct gcgccatttg
    11221 gtttccgaac ggcactcagg ctggcgaatc gaggccgaaa cctgcggtcg gcgttaccgg
    11281 tgtgatcgtt caaagcaggt tcggggggca gatctttggc tacgacattg acgcgaccgg
    11341 gacggaaggt gttttgtgcg aggcacagac gctgcagggc ggcctcgtcc tcgcggccgt
    11401 cgagacattt gatcaaagca ccggccgaat tcttaaggtc ctcgccaaaa gcatggatca
    11461 agggagtgac ttcattaccc tgggcgtagt tggcaattca gtgggcctgg tcgaacgcga
    11521 gcatgtgaag gctttgttca aagtcatccg caccttttcg gtgatcaatc cgcttgacac
    11581 caaccggttc accgccacct ggactccgcc ggttggaacg ggtcacctcg tcgaggaggt
    11641 cagccgcaac cagggcatac caaatgtcgc ggtctgggcg gaagatatca cgagccattt
    11701 tcaaccgttc gtcttcagct caaatgtcgc ggccaatacc tttggaccgg tgatgaccat
    11761 cacggatcag gatttcacct ccggcgccaa ccccggcctg gcctatgacc ctgtgaacaa
    11821 ccgtgcgatt ctcgggcacg ccaccctcgg caatcctttt gttcccggca aaatcgccac
    11881 ggttaatctc gccacggggg ttttcaccaa gttcaccggg gtcggcctcg gtgatgtcaa
    11941 tggcatggcc tacgatccgg tgactggcac tgcctgcacc acgagcgaaa tcgactttag
    12001 cgtggagttc tacaacatcg ccacccagac tggattcgcc gaaccactcc caggcgcgca
    12061 gaatcaattc tatagcgggg ccgacgtgga gttcgacccg gtgaaccggc ttttcctcgt
    12121 ggcccagccc aactcgagca cggcgtcaag cggcagcagc attcacgtct acgatgtttc
    12181 cggcaacctg atcgagtcga tcaatggttt cagtttctcg aacgccttta atgtcgtggc
    12241 ggcacacatc gccctgaacc caggcaaccg cctgggcttt atcgacggcc cggacgccgg
    12301 cgtgacgcag gttcaatcgt tcacctattg aagcgccggc cgtgaccgat gtcttcaatg
    12361 ccgtgacgat cacggagcgg aatcagatca tcacactttt ggattcgctc tagagagata
    12421 ctctcactca gcggctcgct ccggtacgcc cggctagatt cgcgaccgct gcgatcagct
    12481 cctgcggttc gaccggtttt gagagatgca cctgataacc ggccagcatg gcgcgggtgc
    12541 gatcctccga acgcgcgaag gcggtcagcg cgatggcggg gatcttcgcg ctctgcgccg
    12601 accgggcgcg cagctcacgc atcatctcga gaccgtcttt ctccggcatg ccgatgtcgc
    12661 tgacgatgac atcgggcggt tgttcgctcg cgagccgcag gccatcggcg gcggaagagg
    12721 cgagcagcac gtcggctttg cactcctcca atagccgtcg caccaattcg agcgagtcgc
    12781 gttcgtcgtc caccaccagg acgcgcaccc catctaatgg cacattcacc gaccaaccgt
    12841 cggactgact cgcggaggga tgggcccgga ctgagtcgct ttgccggagc gatgtcaagg
    12901 gcagcatgat cgtaaaggcg gagccgttcc cacgaccttc gctttccacc cgcacactgc
    12961 caccatgcaa ttcgacgagg ttcctcacga tcgataaccc cagaccaagg ccgccgtggc
    13021 gccgtgtctg tgaggggtcc tgctgccgga aacgatcgaa aacatagggc aaaaattccg
    13081 gcgaaatccc ttcgccgttg tcgctcacga cgatttccac gtacgagttc acccgctcca
    13141 aaacgacact cacccgcccg cctttgggag tgaacttgat tgcgttcgcg agcaggttcc
    13201 aaagaacctg ttggagtcgc gccggatcgc cggaaatcgc ttgcaccggg tcaagcacct
    13261 tcgtgatcct gacctgcctt gcggcggcgg caggccggag cgcgtcgatg gccgattcaa
    13321 tgactgcggc aagatcagtt ggttggacat cgaggcgagt tttgccggaa atgatccggc
    13381 tcatctcgag cagatcctcg atgatgcgtg tctgcgcgcg cgcgttgcgc tcgattgtct
    13441 cgaggccttc gcgcatggtg tcgttgtcgt cacccgcgcg caggagttgg gcccagccga
    13501 gaatggcgtt gagtggcgtc cgcaactcgt gggaaacggt ggccagaaat tcgtctttta
    13561 agacgctggc gcgatcggcc tccgagcgcg cggcgcgttc gctctcgagc aggcgctggc
    13621 gttcctcgcc atgcatgcgc tgctcggtga tatcggtgtt ggacccaaac cagaaggcga
    13681 ctttgccgtc gccatcgcgc aaaggcatgg cgcgggaaag aaaccaacgg taaacgccat
    13741 cgcggcgacg caggggaaag gtgtcctccc agggctcacg cgtttggagc gcgtgtttcc
    13801 attttgcgat gacctcgggc aggaaactcg ggtcgtgcac tttttgccag ccccaaccgt
    13861 ccatctccac tggttcagtc ccggtgtatt caaaccaccg tcgattgtac caaaagatgt
    13921 gcccatccgg ccgcgccatc caggccagtt ggggaatgtt atccgcgaga taacgaaagc
    13981 gtgcttcgct ctcggccagc ttcgcgccga gcgcgaccga ctcgagtcgc gcccagtgcg
    14041 cccgccgcac cgcccaaacg atcaggacgc aagtgagcag gaagaagctg agattgagca
    14101 gctttcccga atccaacgcc aggggattct cgcccatcca gaacgaagca agcaagagcg
    14161 acaacatcga ggtgatgacc ccggccaagg tcccacccca aagagcggct acgatgaccg
    14221 ccgggaagaa agtcagaaac ggaatcgccg aaccgacaaa gcgaaagaga accgcccgca
    14281 ccagagcagc ggccaaggcg gcgacgactc cgatcgagat aatgcggaat cgttccctcg
    14341 tcaggttggt cacagcggtt caatctccgt ccctttgaat cgtatctgca ccgcccccag
    14401 cgcgtatcgc gcaatggagc gacggccccc cggtgaaccc cgtttccgct gagttggcct
    14461 cgccggcggg agccagcggc catcaactaa cctatggatt gcgagcccag ttgttcctgg
    14521 ggcttgcgat gccgcggtaa atccgcgtcg agaaacccga gcgcgtgcgg aggcttgatc
    14581 acacgcacgc cggcggcgcg ctgttccgcc gcgatctgat cgtggaaggc ctgcatccgc
    14641 gtccaatgta ccttcggccg gaaatggtgc tccgcgtgat agccgttata gaaccacgtc
    14701 cagttgtaga gcttctcgta gctgctcacg ccccaggcga tcggctcgtc gggattcccg
    14761 ccataatgcc ggtaatagcc gttcaagtag gagaggctgt gcccgagata ataaaacggg
    14821 ataaggaaac aaatgtaacg ccagttcaga atgccgagga tgacgaaggt cgtgacaaaa
    14881 agcgtcaact cgaccacgcc ccagaatgct tcccgcggat tctttcgttt cagctcgcga
    14941 tagaccgtct tgggatcttc gcgaaagaag ctgaagaagg cgtatttgaa agggtgctcg
    15001 gcctcgccct cctcgccgtg cttgtaaatg gaaatccagt cgatcgtctc tcccttttcg
    15061 tcaggccgat cggcgtttcc acggtggtgc tgcatgtgaa tgcactcata gaaaatctgg
    15121 ctaaacccga cggtgatcga ctccaggatg ctgaagagcc ggttgaggag cggcgaccgg
    15181 aaatagggat tgtgaatgaa gttgtgcgaa atgccgttga tgttccagga aatgctgacg
    15241 gcatagatga atccgaggat gagcatgatc caaagcggcg tgcgcgggaa aagataaaac
    15301 agaccgaaaa aatacgcaca gtggaggagc gccgcgacca gcgggatggc gtcccagcgc
    15361 gtgtaggcga agagtttcga acccgtcgtc aggcgttcca ctgcgatgtc gtttgttttc
    15421 attacaaagt cctccgcggc agccactgaa accaccctgc tcttccctgc ctgcggttaa
    15481 gggaatctct gcgagacccc caaggcgcgt caagtcctac gccgtcttct cgtagcgccg
    15541 catcagcttg aagaaccgga tcgagtcgcg caccgggtgg atgctgctca cttcgtcgga
    15601 gtagatcgtg ctgatcggca ccgaggcgat gcggaatccc gcgcgactgg cgatgatgag
    15661 catttcggtt tcataatcga aacgactcgt gcccgcgagg agatggggag cgagcgtccg
    15721 atggatcatg cgatagccgc attgcgtgtc gggcacttcc tggccacaga gccggctgat
    15781 gcgccgactc atgtagcggt tgaccgtccg cctggcgaaa ggcatgtgac gcacgtcgtg
    15841 catccgggtg ccgatgaaaa ggctcgcctc actcgcggcc acggcggcaa aaaagcggct
    15901 gatttcctcc ggcaaatgct ggccatcggc atcgaagagg aggatgaatt cgatctccgc
    15961 ttgggcgagc caatgacgca aaccggtttt gatcgcctcc cctttccccc ggttctcggg
    16021 gtgcacgatg acttccgcgc cgccttcccg cgcgcgggct gcggtcgcgt cgcccgagcc
    16081 gtcgtcgatg acgaggacac gatcgagttg cgcgcgggcg cgttctgcga ccccgcgcac
    16141 gtggaccgcc tcgtgatagg ccgggatgac ggcggcgaca ttggcgcgat ccagcggccg
    16201 actggttttc ggcgccccgc tctccatcgt tagtggacga ccggatgaaa atccgccgcg
    16261 tgataggagc tgcggacgag cggcccggag gcaacgtggg cgaagccttt gccgcgcgcg
    16321 atgtcgccgt agagatcgaa ttgctcgggc gtgatgtaac cgacgaccgg gaggtgttgc
    16381 ggcgtcggac gcagatattg gcctaacgtc agcacctggc agccgacttc gcgcaaatcg
    16441 tccatcgcct ggaaaagttc cggttccgtt tcgcccaggc cgagcatgac gccgcttttc
    16501 gttacgatgt ccggcgcaag ctccttcgcc cgacgcaaga cctcgagcga ggtgcggtat
    16561 ttcgcccggg aacgaaccgc cggtgtgagg cgctcgaccg tttccaggtt gtgattgaaa
    16621 atctccggcc cggcgtcgag cacgagttgg atgcaccact cctgggcatg aaaatccggc
    16681 acaagaactt cgatgatgat ggagggatcc atcgcgcgaa tggcgcgaat ggtgcgcgcg
    16741 aagtgttccg cgccgccgtc cttcaaatca tcgcgcgcca cggcggtaat gacgacgtgc
    16801 ttgagtttca agcgcctgac cgcctcggcc acgcgctgcg gctcgtcttc ctcgagcgcg
    16861 aacggcttgg cggtcgtgac cgcgcaaaag ccgcaggccc gggtgcaacg ttcgcccgcg
    16921 atcatcatcg tcgcggtccc ctgactccag cattcccagc ggttcgggca ttgcgcgctt
    16981 tcgcacacgg tatgcaaacg cagatccgag atgagcgcct tggtcgagaa aaagacgggg
    17041 ttggaaggca gccgcacttt gatccagggc ggcttccgcg cctggtgcaa tgcgggatcg
    17101 atcttggcgc aggacatgtt cggcgatttt cggcagttct aactcttagg cagggaggcc
    17161 gcaaatccct cttaacacta aagctcaaaa cttaaagctg cgagctgaga gaatggttgc
    17221 ttccaccgcc caaatctgat tacgatgcgg ccgaacattt ttccgcagcg aagtttagca
    17281 aacaccagga gttggaccat ggaaggacaa gataacgaaa tgacacacgg gcaggagaac
    17341 ggcgcgatcg acgaaagcgg caaggtcgat cgcatccgtg acattctttt tggctcgcaa
    17401 atgcgcgatt acgacggccg ctttcagaaa ctcgaggaac gcctgacgcg tgaagcggac
    17461 gagttgcgcg gcgatttgca gcgccaactc gaggcactcg agggcttcat gaaaggcgaa
    17521 gcggaatcga tgaccaaccg cgtcaagacc gagcagtcgg aacgcggaca ggcctgcgag
    17581 cggctcgcgc gtgagatggc tgaaaccgcg cgcgctctgg agttgaagat cggcaatctc
    17641 gacagccagg ccgccaagga tattcgcgag ttgcgcgagc agttgctcga gcaatcgaaa
    17701 gccctgagcg cgcagctgaa ggagaagcac gatcatttga aggcgcagct cgatcatgaa
    17761 gccggccaga tccgcgacgc catgactggc cgcgaagccc tggccgagat gctgaccgag
    17821 gtcgcgctcc ggttgaaaaa agaattccgc gtcccgggca catcatagaa gatgcaccct
    17881 cggatgcgga gcgcgcagcc gcgaaagccg gtggatgacc tggctgagtt gcgccggctg
    17941 atcaaccagc cggagcagga taagctgcgc gacctgcgcg atcggctcga tgacaaagag
    18001 cagcgcgctc acgaggtcgc gtcgatcctg ccggaagccg tcactctgag cggcgatcgc
    18061 agcgaagagc tgacgcgcgc cctgcggccc gcggtcgagg gttcggtgcg cgagtcgatc
    18121 gagaagcgcc ccgaggtttt tatcgacgcg ctccatccga tcatcggctc gatcgttagg
    18181 cgatcgatcg cggaaagcct gcgcacgctc ctccaatcgc tcaaccaggc actcgagcaa
    18241 agcttttcct ggcagggatt gaagtggcgc ttcgaggctt tccgcaccgg ccggagcttc
    18301 gcggaagtcg tcatgctgcg cagcctcatt tatcgggtgg aacagatctt tcttattcat
    18361 cgcgagacga gcctctcgct cctccacctg agcgccgacc cggccgtcgc gcaggattcc
    18421 gacatggtcg cgggcatgtt gagcgcgatt caggatttct cgcacgactc cttccagacg
    18481 gggaaggacg aggcgttgga agagtttcgc atcggcgagt tgcaggtttg gattgctccg
    18541 ggggcacacg cttatctcgc ggccgcgatc cgcggcaatc cgccgcgcga actgcgcgcc
    18601 tctctcgagg atgcgatcga gcgggtgcat gtcttgcgcg gttcggcgct ggcgaagttt
    18661 aatggcgatg cggcgcagtt cgaatcgctc cgatcggagt tggaaccgtg tctgcgcgcg
    18721 caatatcagc cgcagaagac ggccggccgg accaaccggg cctggtttgc gcttgcggcc
    18781 ggcgcggggt tggtcgtggc cgccctgatc gccgccacgc tgagcgaaat gcgctggcgg
    18841 gatttcgtta ggcgactgaa ctctgcgccg gggatcgcgg tgacgcaggc ggaaaaaggt
    18901 tggatctcaa aatcgcgggt tgcatttcta cgcgatcccg aagtggccga cccggccgcg
    18961 ctggcgcggg ccgccgggat caatcccagc cgtatctact ttgagtggaa agattacctg
    19021 gcgctcgatg atgcgagcgt ccggcggcgt ttcgagagac gattcgggaa accggccggc
    19081 acgaaacttg agctgacgaa tggcgcactg cagatcagcg ggaccgtccc ctacgaatgg
    19141 ctggcccgcg tgcagcagga cggcgtccaa tcgcccggcg tcacctccat cacagacaag
    19201 gatgtgaaag tgacctacga tccggatttg gccctcggga gatttcagcg cgcgtttccg
    19261 ccgccggcgg gtgtgaccgc gaaggtggag aacggaacgc ttctcctcgc cggcacggct
    19321 ccttacgaat ggctcgcgcc ggtgcgcgaa aacgcggtca aattgcccgg gattaaccgg
    19381 atctcggaag aacatttgca ggtcgcctac gaccccaaac tcgtgctcca gcgttttagc
    19441 gatcgcttta gcctgccgga cacggtcaac gccacgatga aggatggcat ccttgtcctt
    19501 tccggcgaag cggcgcatgc ctggttgctg cgggtgcgac caggtgcgcc gaccgtcccg
    19561 ggcatcaaga cgatcgacga acgcagcttg accgatctcg acgtgcgcgc gttccagcaa
    19621 tcgaagtcgg tcatcgagag cgcgtttgtt tattttcttt tgaacaaaga caacttcgcc
    19681 acggaaggat ttgccgcgct ctcgcgttta ccggatgaga tccgtcgctg cctgacggcg
    19741 gccaatcggc tcggactcga aattcaaatc gaggtgcgcg ggtccgcgga tgcggtcggc
    19801 agcgggaaga ggaatgccga actgagccag cgtcgggcgg aagcggtccg cgactttctg
    19861 gtcaactgcg gtttcgcgtc atccctcttc aagacaatgg gcttgggcgc gcctgccaca
    19921 gcgggtcagc cagcgccgga acaagcagat cgccgcacgt cgttcaagat cgtcccgaag
    19981 tcatgattca gaaaaaagtc tgcctgctgg ggacttccgg ggtcggcaag acaagcctcg
    20041 tggcacagtt cgtccacagc atgttctcgg ataaatacct cactacggtg ggcgtgaaga
    20101 tcgacaagaa aacgctgccg gtggacgaca ccgaagtcat gctcgtgatc tgggacttgg
    20161 cgggcgacga cgatttccag cggttgcaaa cctcctatct gcgtggcaca agcggctacc
    20221 tgctggtggc ggacgggacg cgacgggtca cgctcgatca ggcgctcgaa ttgcagaagc
    20281 gagtcgcgga aaccctcggg gcgacgcctt tcgtgctcgc gctgaacaag gccgatctgg
    20341 caccgcaatg ggaagtggat gaggcacaga tcagcgcgct cgccgcacag aatttcacga
    20401 tcattaaaac gagcgcgaag gaaggcgccg gtgtggagga gtccttcgcg caccttgccc
    20461 gccggatgat ggcgcccgcc taacgatcat gcccgacgcc gaatcgcccg cccaatctgt
    20521 cctcgaagcg ctcggcttcg tgctcttcgc gcgcgagagc ccgggcactt tgcgtttgtt
    20581 aggcaaagca cccgcctggc tcggcgctct ctggccctcg ctccgcgaaa gcggcgggac
    20641 tctgccggtc gcggacgcct cgcccttcct ggaaaacttc ctggtcgacg ccgaagaatg
    20701 ctggggcaaa ggtggcggcg agcgcgcgaa gtcgggaccg tgggtggaaa acgggagcgg
    20761 cgcggaggtc gaactggagg cgaccgcgct
//
LOCUS       DATMNS010000237         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_700515, whole genome shotgun sequence.
ACCESSION   DATMNS010000237 DATMNS010000000
VERSION     DATMNS010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700515"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VNW28_08350"
     CDS             complement(<1..187)
                     /locus_tag="VNW28_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09978.1"
                     /translation="MAPESENNLPLEIGHVLFIDIVGYSKLLIEEQKERLGQLTKIVL
                     GTAQVRDSTDEQLVRLPT"
     gene            complement(498..1514)
                     /gene="purM"
                     /locus_tag="VNW28_08355"
     CDS             complement(498..1514)
                     /gene="purM"
                     /locus_tag="VNW28_08355"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HXA09979.1"
                     /translation="MTKKAYARAGVDVDLGNRLKRRIQGFVRQTHGPEVLGKIGGFGG
                     LFAPNFTGLREPVLVSSVDGVGTKLKIAFALDRHDTVGADLVNHCINDIAVVGAKPLF
                     FLDYIGAEKLESRVFAQILRGFSKACRAGGCALIGGETAQMPGMYRRGEYDLAGCIVG
                     VVERARMIDGSRIRPGDVILGLPSNGLHTNGYSLARKIFFEQMRLKPAARIPGLRLSL
                     GEELLRTHRNYQPLLAKVPPGKLKGLAHITGGGLLDNLPRILPKNCDARIEIRKWTVP
                     PVFQIMQDRGRVLREEMYQVFNMGIGMAAIVSPNDAAAVAKQLRAKPIGRIERGRGIT
                     RLSS"
     gene            complement(1612..3117)
                     /gene="purF"
                     /locus_tag="VNW28_08360"
     CDS             complement(1612..3117)
                     /gene="purF"
                     /locus_tag="VNW28_08360"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXA09980.1"
                     /translation="MSIFAAFMTLEELALPSPPPYPMHECGIFAVFGHPNAAVLTYYG
                     LFALQHRGQESAGIVTSNGPGSSFLLHRDMGLVSQVFGQDALQRLKGSRALGHTRYST
                     TGSSTLKNAQPFVVDCFRGQLAIAHNGNLINADVLRDELERKGSIFQTTADSEIILHL
                     LAQPSNNGGNILTALRRIEGAFSLIIMSEREIIAVRDPFGFRPLSLGKLDGAYILASE
                     SCAFDLIHAEFIREIEPGEVLIISEDGLRSEWPFREEKPAFCMFEYVYFARPDSIIGG
                     VNVAKVRTEMGRRLAREFPVEADIVVPVPDSGNYAALGFAHELKIPYEHAFVRNHYIG
                     RTFLQPSQLIRDFDVRVKLNLIKEAVEGKRVVVVDDSIVRGTTARARVVNLREAGAKE
                     VHMRVSCPPHRFACHYGIDFPDPEKLIANRKTMPEICEYLGVDSLGYLGLEGMIEATG
                     KPANSFCLACFTGKYPLPVDPELDKFIMEKREARAKALAEEAQPTLFAGLK"
BASE COUNT          619 a          985 c          978 g          630 t
ORIGIN      
        1 cggtcggcag cctaacgagt tgttcgtcgg tcgagtcgcg cacctgcgcc gtcccgagca
       61 cgattttcgt cagctggccg aggcgctcct tttgttcttc gatcaagagc ttcgagtaac
      121 cgacaatgtc aataaacagc acgtgcccga tctcgagcgg aaggttgttc tcagattcag
      181 gcgccacgtc gttattttac aggggccgaa agcccttcac gatcattttc cgtcaccgaa
      241 gactcaacgg cgcgtgggtc gttcgagcac gccgcgtttg gagcgtccaa tgcaaagcgc
      301 aaggcgatcg cttagccgcg cgagcgcttc gggggagctg cgggcgaagt gcgccgattc
      361 gccgatgaca ttacactccc gataacccaa gaatccatct gatactgcca tctgatagat
      421 ctcaccagtt acgctcggtg tcttctccga tccggcgttc gaacgtcact tgcgaggtcc
      481 atcgccgtct gcacggctta ggacgacaag cgggtgattc cgcgaccccg ctcgatccgc
      541 ccgattggtt tggcgcgcaa ctgcttcgcc acggccgcgg cgtcgttagg cgaaacaatg
      601 gccgccatcc cgatccccat gttgaagacc tgatacatct cctcgcggag aaccctgccg
      661 cgatcctgca taatctggaa aacaggcggc actgtccact tgcgaatctc gatccgcgcg
      721 tcgcaattct tcggcaaaat gcgcggcagg ttgtcgagca agccgccgcc ggtgatatga
      781 gccagacctt tcagctttcc gggcggcact ttggcgagca atggctggta gttgcggtgc
      841 gtccggagca gctcttcccc taacgacaag cgcaagccgg gaatccgggc ggctggcttc
      901 aggcgcattt gctcgaagaa aattttccgc gccagggaat agccattggt gtgcaagccg
      961 ttcgacggca gcccgagaat cacgtcgccc ggcctgatcc gcgaaccgtc gatcattcgc
     1021 gcccgttcga ccacgccgac gatgcagccg gcgagatcgt attcgccgcg tcgatacatc
     1081 ccgggcattt gcgccgtctc gcccccgatc aaggcacaac cgcccgcgcg gcaggctttg
     1141 ctaaaaccgc ggaggatttg ggcgaagacc cgcgactcca gtttctccgc cccgatgtaa
     1201 tcgagaaaaa agagcggctt cgcgcccacc accgcgatgt cgttgatgca gtgattgacg
     1261 agatcggcgc cgacggtgtc gtggcgatcg agcgcgaaag cgatctttaa cttcgtgccg
     1321 acgccgtcga cgctcgagac cagcaccggc tcgcgcagcc cggtaaagtt cggcgcgaac
     1381 aagccgccga agccgccgat tttgccgagc acctccgggc cgtgtgtttg cctaacgaat
     1441 ccctggatcc tgcgcttgag tcgattgccg agatcgacgt cgactccggc gcgagcgtag
     1501 gcttttttcg tcattccgag cggagcgcag cggagccgag gaatcccgtg atgtcagcgt
     1561 taagccgacg ccccgggatc cctcggctgc gctcgggatg acggactcgc attacttcag
     1621 cccggcgaac aaggtcggct gcgcttcttc ggcgagcgcc ttggcgcgcg cttcgcgctt
     1681 ttccatgatg aatttgtcca gctcgggatc gactggcagc ggatatttgc ccgtaaaaca
     1741 ggcgaggcag aaactgttcg ccggtttccc ggtcgcttcg atcatgccct cgagaccgag
     1801 gtaaccgaga ctgtcgaccc cgagatattc gcagatctcc ggcatcgtct tccggttcgc
     1861 gatcaacttt tccggatcgg gaaaatcgat cccgtaatgg caggcgaagc gatgcggcgg
     1921 acagctcacc cgcatgtgca cttccttcgc gccggcttcg cgcaggttga ccacgcgggc
     1981 ccgcgcggtc gttcccctaa cgatcgagtc atcgaccacc acgacgcgtt ttccttcgac
     2041 cgcttccttg atcaggttga gtttgacgcg cacgtcgaag tcgcgaatca gctggctcgg
     2101 ctgaagaaag gtgcgcccga tgtagtgatt gcggacgaag gcgtgctcat agggaatctt
     2161 gagttcgtgc gcgaaaccaa gcgcggcgta attcccggaa tcgggcacgg gcacgacgat
     2221 gtcggcctcg accggaaatt cgcgggccag ccgccgaccc atttcggtcc gcactttcgc
     2281 gacgttcacc ccgccgatga tgctgtcggg tcgcgcgaaa taaacgtact cgaacatgca
     2341 aaaggccggc ttctcctccc gaaacggcca ttccgagcgg aggccgtctt cgctgatgat
     2401 gaggacttca cccggctcga tttcgcggat gaactcggcg tggatgagat cgaaggcaca
     2461 gctttcggaa gcgaggatgt aagcgccgtc gagcttgcct aacgacaagg gccgaaaccc
     2521 aaacgggtcg cgcacggcga tgatttcgcg ttcgctcatg atgatgagcg agaacgcgcc
     2581 ttcgatccgg cggagcgcgg tcaggatgtt gccgccattg ttggaaggct gagcgagaag
     2641 atgaaggatg atttcgctat ccgcggtcgt ctgaaagatc gaacccttgc gctcgagttc
     2701 gtcgcgcaga acgtcggcgt tgatcaagtt tccgttatgc gcgatcgcga gctgcccgcg
     2761 gaagcaatcg accacgaagg gctgcgcatt cttcaacgtg ctcgagccgg tggtggaata
     2821 gcgagtgtgg ccgagggcgc gcgagccttt gaggcgttgc agggcgtcct gcccgaaaac
     2881 ctgcgagacc aggcccatgt cgcgatgcag caggaagctg gagccggggc cattcgaagt
     2941 cacaatgccc gcgctttcct ggccgcgatg ttggagcgca aagaggccgt agtaggtcag
     3001 gacggcggcg ttcggatgcc cgaagacggc gaaaatgccg cactcgtgca tcgggtaggg
     3061 cggtggcgat ggcagggcaa gctcttccag cgtcatgaag gcggcaaaga tactcattct
     3121 gcgcgcaact tcatcttccc gccccgattt catttccctc tccagccgcc atcccgacgg
     3181 attggcgggc ttacgtgcgc gcatcggcag gt
//
LOCUS       DATMNS010000238        10647 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_349384, whole genome shotgun sequence.
ACCESSION   DATMNS010000238 DATMNS010000000
VERSION     DATMNS010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 10647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10647
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349384"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1188)
                     /locus_tag="VNW28_08365"
     CDS             complement(<1..1188)
                     /locus_tag="VNW28_08365"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HXA09981.1"
                     /translation="MRFLFFGLLVASLVGWLFTYQRQRWRIRALERSNEEIQVEESLV
                     FDFLHGLGEAFRDVIRPHDLHRLIVESSVRILDAHGGALYMVDRTGTKLVPAFISKGC
                     PPLVEVPDRILQQAATAPIALDGFLRSHPISAGEGLVGRVWQKGEALLLGDLADAPEL
                     SKLRDTAHGTASVMVAPLLYGRQNLGVLAVANGPMSATFSPSEFIVFKSISEQSAFAL
                     YNAIVYSEANEKKRLDHDLQIARDIQRILLPSQPPTIEGFDIAGINIPARQVSGDYFD
                     YIKVDDDRLGVVIADVSGKGVPASLIMAICRSVLRAEARLSQSPAEVLRKVNRQLYPD
                     IKEDMFISMAYVILDFKNHHVTLSRGGHDAPLLYRGATGEVEVLKPPGMALGIDSGDV
                     FDRI"
     gene            complement(1204..1725)
                     /locus_tag="VNW28_08370"
     CDS             complement(1204..1725)
                     /locus_tag="VNW28_08370"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXA09982.1"
                     /translation="MQSSIQVGVNGPVVWVRVAGRGSFLESGSLKEFAREMVNRGYRE
                     FVFDLEECVMMDSTFMGTMAGMALRLKELGGGHLRVVHCGQRSRDLLSGLGLDQIFEI
                     QADGMTAPECEELAAKRGEASGKAEAAKRDQAGTMLEAHEALCRAAPENLTRFKDVLE
                     YLKQDLQHESPAK"
     gene            complement(1902..2414)
                     /locus_tag="VNW28_08375"
     CDS             complement(1902..2414)
                     /locus_tag="VNW28_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09983.1"
                     /translation="MRNLLVLLCLAFGGCAGTIPRPYQVETPKPLPVALDRDFEIRKT
                     GQYFLDPAPKPITGQIDQSVTFERGYRMYGAVTAVDQHQRFGNYFDFFWRARRSADVR
                     VRLEYRQEKLHALVQAREVHYLHARGNHKTEFAIVGDDFFDDGRVIAWRASLIVDGRV
                     AAVTRSYLWE"
     gene            2621..5308
                     /locus_tag="VNW28_08380"
     CDS             2621..5308
                     /locus_tag="VNW28_08380"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXA09984.1"
                     /translation="MISVHYEQGVFLPNENIWLDPSEAKPFAFVSHAHSDHIAPHDEI
                     IVSERTSRLLQARLPGKRHEHVLPFGRPATVRGLEVTLLPAGHIFGSAQFFLESDAGS
                     LLYTGDFKLRRGRSAEATEWKQADTLIMETTYGRPRYRLPPTELVVQQIVAFCREALE
                     EGATPVLLGYSLGKAQEILCSLAEANLRPMLHGSVYRMTRIYEQFDQAFCQYERYDAK
                     GVAGKVLICPPSANRSRMLEKIPKKRVAMISGWAVEPNAVYRYQVDAAFPLSDHADYD
                     DLLRYVELVQPKRVLTLHGFAADFARDLRERGLEAWALSEENQLELTLRSAPPRKVRS
                     VTPSAGAGSRPAAVRSEFAELAQLGEQIAATPAKLEKIRLLSEYLRALDPEGLPIAAI
                     FLTGKPFAQTDQRTLQTGWAIIFRALLGASRRGDAELRRIARSHGDASKSAREALEGR
                     TTPEPFSLGEAHALFEALHKTRGPAGKTELLQARLQKLSALEAQYVVKILTGDLRIGL
                     REGLVEEAIARAFDAPLDDLKEAHMLLGDIGQTALLAARRELDRAELTLFRPLKCMLA
                     SPEPTAEAIWARFADKSQTTYVEDKFDGIRAQLHRGVDRVELFSRDLRRVTGQFEELA
                     EGAGGLGDEVVLDGEIIAFERGEKLTFFDLQKRLGRKSESADLFAAPTADVPVVFVAF
                     DLLWLNGRSLLQTPLRERRELLRGLTMPAQFQIAEVSPAGSAEEIELAFQLARRRLNE
                     GLMIKDPESPYTPGRRGLSWFKLKKELATLDVVVVAAELGHGKRNHVLSDYTFAVRDE
                     ESGMLLPIGKAYSGLTDVEIAELTEHFRRNTTSDHGRYLEVRPDTVLEIAFDSIQPST
                     RHASGLALRFPRIKAIRRDKTPDAIDTLAYARQLADATR"
     gene            complement(5305..5595)
                     /locus_tag="VNW28_08385"
     CDS             complement(5305..5595)
                     /locus_tag="VNW28_08385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09985.1"
                     /translation="MRRILLLPLFAALAAFVSSCGKRPTEAERNAEIERQVQERLNAE
                     HRADEQKKLAQRQAEPDAREQALVAEENRFAANATQAAAELSSARTAVADQP"
     gene            complement(5665..5871)
                     /locus_tag="VNW28_08390"
     CDS             complement(5665..5871)
                     /locus_tag="VNW28_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09986.1"
                     /translation="MELPGLPARSPGTSRAPASGLKLMLAILTVFALLAGYGQWQRLQ
                     RPKIETVTIIPVPGAPASPSPNER"
     gene            complement(6016..6630)
                     /locus_tag="VNW28_08395"
     CDS             complement(6016..6630)
                     /locus_tag="VNW28_08395"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXA09987.1"
                     /translation="MNPWYFTIYHLTRLIARVLFRFRVIHPERVIQEGPVILAMNHES
                     YLDPPFAGIACRRAIYFLARKSLLDVPVLGWVLPKLNVIPVDQEGGDRSALKALIRTL
                     RVNHGALVFPEGSRTLDGQLQPPQPGLGFVIAKTLAPVVPMRIFGAHRALPRGGGKLR
                     FCPITIVVGEPLHFTAADLAGIGREVYPRLSARVMEAIAALKLE"
     gene            complement(6627..7289)
                     /gene="cmk"
                     /locus_tag="VNW28_08400"
     CDS             complement(6627..7289)
                     /gene="cmk"
                     /locus_tag="VNW28_08400"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HXA09988.1"
                     /translation="MNAPSAHKIIAIDGPAASGKSSVARELARRLGFVYVNSGAMYRA
                     ITWLVLERGIPADDAAAIEQLAGSAAIACELRDRDSFVRIDGVDPEPFLREDRVNDNV
                     SLVSSVPRVREILVDHLRRYAVTNDLVMEGRDIGSVVFPETPFKFYIDASPEIRLRRR
                     AAQGQRDQVTARDEADSSRRASPLIIAEDAHVIDSSNLTIDGVVGEIIGRLKLKGLTI
                     AR"
     gene            complement(7314..8666)
                     /gene="aroA"
                     /locus_tag="VNW28_08405"
     CDS             complement(7314..8666)
                     /gene="aroA"
                     /locus_tag="VNW28_08405"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HXA09989.1"
                     /translation="MDDLRIKRAPRIEAEITVSGDKSISHRAVLIASLANGPCVLRGF
                     SRSADCMNTVVAMRALGITIEQPEPEVLIVHGKKRKLTAPQADINCGNSGTTMRLLAG
                     MLAGQPFKSRLVGDGSLSERPMARILEPLRLMGAKIAAEGEHETAPLRIEGAPLHGIK
                     YSMPVASAQLKSALLFAGLFAKGKTTVQEPVPTRNHTELMLNYFLIRTHKDETGVSVF
                     GDHVPESRDFSIPGDISSAAFWLVAAAAQPGGHLLVREVGLNETRTGLLGVLVRMGAQ
                     VREAIEDVDQVEPRGIVEITGARLKATVIQGKEIPQLIDELPILAVAGALANGTTIIR
                     HAQELRVKETDRIAAIAHNLRSMGGQVVELTDGLEIHGGTPLHGTRLSSFGDHRIAMA
                     FAVAGMFAEGETVIQDVDCVASSYPGFEKMLSELGNPKGFRTTTPVISSLSHSRAEEV
                     "
     gene            complement(8809..9195)
                     /locus_tag="VNW28_08410"
     CDS             complement(8809..9195)
                     /locus_tag="VNW28_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09990.1"
                     /translation="MKKSISLALLTTLFVAACSEPEPPPRRPHATTYQQAPETQYPTQ
                     QPFNQGTPPPPEVTTTPPPPPQTAATAAPVKPTKGDVPYGIPVPDKPGFVKSPYAPDA
                     GLVDVRGFPPGSEVRDPYTQKIFLVP"
     gene            9317..10501
                     /locus_tag="VNW28_08415"
     CDS             9317..10501
                     /locus_tag="VNW28_08415"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused response regulator/phosphatase"
                     /protein_id="HXA09991.1"
                     /translation="MLFESKKEMERREEVILVIDDDRGSRRMLLRALGEAGYACRESA
                     SGVEALEMLHAEAPSLLLLDFDMPELNGAEVLSRLREDADPAIAQLPAIMLTGFGGRA
                     TEVHCLEAGANDFVTKPINLPVLRARIDTQLRLQSLRRQLQQQNDELEAWRKDLERDL
                     AAARLTQRSLIPQKPPLLPGWEVAAVYRPVIQVGGDIYGWLRMTDGRTLFWIADATGH
                     GAAAALLTTLAKLLFHHGSGEHARPAEIMEAVNNDFRSIFGARSFMTAMCVALEAQTG
                     CASVVGAGHPPLIVARRAGAAELVTSIAPPLGLLEKSHFEETTIALQPGDGFLLYTDG
                     LYGASKVAAERWTPTRLQALMTDDHSSAQALLGHLLERAAPDKGEPQADDLAALVVRR
                     GS"
BASE COUNT         1986 a         3249 c         3451 g         1961 t
ORIGIN      
        1 tatcctatcg aacacgtctc cgctgtcaat tccaagggcc atcccgggcg gcttcaggac
       61 ctccacttcg cccgtcgcgc cgcggtagag caagggggca tcgtggccgc cacgagagag
      121 cgtgacgtgg tggtttttga aatcgagaat cacgtaggcc atgctgataa acatgtcttc
      181 cttgatatcg ggatagagct ggcgattgac cttccgtaaa acctcggccg gcgactggct
      241 gaggcgcgct tccgcccgca gcacgctccg gcaaatcgcc atgatcagcg acgccggcac
      301 gcctttcccg gaaacatcgg cgatgactac gccgagccgg tcgtcatcta ccttgatgta
      361 gtcgaaataa tcgccgctca cctggcgcgc tggaatgttg attccggcga tatcgaagcc
      421 ttcgatggtg gggggttggg aggggagcag gatgcgctgg atgtcgcgcg cgatttgcag
      481 gtcgtgatcg agccgtttct tttcgttcgc ctccgaatag acgatcgcgt tatagagggc
      541 gaaggcggat tgctcggaga tcgatttgaa aacgataaat tcgctcgggc tgaacgtcgc
      601 gctcatcggg ccgttggcca cggcgaggac gcccaggttc tgccggccgt agagcaacgg
      661 ggcgaccatg acggacgccg tcccgtgggc ggtgtcgcgc aatttcgaca actccggcgc
      721 gtcggcgagg tcgccgagca gcagcgcttc gcccttttgc cagacgcggc ccacgaggcc
      781 ctcgcccgcg ctgatcggat gggagcggag gaaaccatcg agcgcgatcg gggcggtcgc
      841 agcttgctgc aggatacggt cgggcacttc caccagtggc gggcagccct tggaaatgaa
      901 ggctggcact agcttcgtcc cagttcgatc gaccatgtag agcgcgccgc cgtgcgcatc
      961 cagaatgcgg accgagctct cgacgatcaa ccgatggaga tcgtgcggcc ggatgacatc
     1021 gcgaaacgct tcgccgaggc cgtggaggaa atcgaagacg aggctttcct ccacctggat
     1081 ctcctcgttt gagcgctcga gcgcgcggat gcgccagcgt tgccgttgat aggtaaacag
     1141 ccagccgacg agcgaggcga caagcagccc aaagaaaaga aaacgcatgg agccgaaggc
     1201 ggactacttc gctgggctct cgtgttggag gtcttgcttg aggtattcga ggacgtcctt
     1261 aaaccgggtc aagttttccg gcgccgcccg gcagagcgcc tcgtgcgcct cgagcatcgt
     1321 gccagcctgg tcgcgtttgg ccgcctccgc tttcccagac gcctcgccgc gctttgccgc
     1381 cagctcttcg cactccggcg ccgtcatccc gtcggcttgg atctcgaaaa tttgatcgag
     1441 tcccaacccc gaaagcagat cacggctcct ctgaccgcaa tgaactacgc ggaggtggcc
     1501 acccccaagt tccttcaggc gcagggccat tcccgccatc gttcccatga aagtcgaatc
     1561 catcatcacg cactcctcca agtcgaagac gaattcgcga tagcctctat tcaccatctc
     1621 ccgggcaaac tccttcaggc tgccgctctc gaggaagctg ccccgccccg ccacccgcac
     1681 ccagacgact gggccgttga cgcctacttg aatcgaggac tgcacgacta actaaagata
     1741 aaatttcgcc tgagactgtc aatccagcgg gccgctttat ccggcctgaa cattttgggc
     1801 tcctccgcct gacggggacg ccgaagcggt tcgggttggg agcggcggca agacatttgc
     1861 atcatcaaaa agaacaggag tggaaatgcg ccatttgccg ctcactccca aagatacgat
     1921 cgggtgaccg cggcgacccg gccgtccacg atgagcgagg cacgccaggc gatgacccgt
     1981 ccgtcgtcga aaaaatcatc gcctacgatc gcaaactccg tcttatgatt cccgcgagcg
     2041 tggaggtaat ggacctcgcg cgcctgcacc agggcgtgca acttttcctg ccggtactcg
     2101 agtcgcaccc ggacatcagc ggagcggcgg gcgcgccaaa agaagtcgaa gtaattccca
     2161 aaacgctggt gttggtcgac cgcggtgacc gcgccgtaca tgcgatagcc acgctcaaaa
     2221 gtgaccgatt gatcgatctg cccggtgatc ggcttcgggg cgggatcgag gaaatactgc
     2281 ccggtcttgc ggatctcgaa gtcgcggtcg agcgcgacgg gcaacggttt cggcgtttcg
     2341 acctgatagg ggcgggggat tgtcccggcg cacccgccga aggcgaggca aaggaggacc
     2401 agcaggtttc tcacgacggg agcatcaaac gagatgagcg caccggtgtc aatcgccggc
     2461 catcgctggc ctgagtcgtc tcctcccgct cgtgctcgtg atcgaaaaag ttgagggcga
     2521 tcgattacga ttacgagcat gagcacgagg atgaggggaa gccgggcgag ggtcccgctg
     2581 ctccattcgc gcgcgcggcg ggatgccgta aattttccgc gtgatttcgg tgcactacga
     2641 gcagggcgtt tttttgccta acgaaaacat ctggctcgat ccgtcggaag cgaaaccgtt
     2701 cgcctttgtc tcgcacgcgc acagcgacca catcgccccg cacgacgaga tcatcgtctc
     2761 ggaacgaacc tctcgattgc tgcaggcgcg gctccccggc aagcgccacg agcacgtgct
     2821 cccgttcggc cggccggcga cggtccgcgg gctggaggtc acgctcctgc ccgccggcca
     2881 catctttggc tcggctcagt tctttctcga atccgacgcc ggcagcctgc tctacaccgg
     2941 cgatttcaag ctgcggcgcg gccgttctgc ggaggcgacg gaatggaagc aagccgatac
     3001 cctcatcatg gaaaccacct acggccggcc gcgctatcgt ctcccgccga ccgagttggt
     3061 ggtgcaacaa atcgtcgcct tctgccgcga agcgctcgaa gaaggcgcga cgccggtgct
     3121 tctcggctat tcgttaggca aagcgcagga gattctttgc tcgctggcgg aggcgaacct
     3181 ccggccgatg ctccatggct cggtctatcg gatgacgcgg atttacgaac aattcgacca
     3241 ggccttttgc caatacgagc gctacgacgc caaaggggtc gcgggcaaag ttctcatttg
     3301 tccgccgagc gcgaaccgct cgcgcatgct cgagaagatt ccgaagaagc gcgtcgcgat
     3361 gatcagcggc tgggcggtcg agccaaatgc cgtttaccgt tatcaggtcg acgccgcctt
     3421 tcctctctcg gatcacgccg actacgacga cctgttgcgc tacgtggaac tggtgcagcc
     3481 gaagcgtgtc ctgaccctgc acggattcgc cgccgatttc gcgcgcgacc tgcgtgagcg
     3541 cgggctcgag gcgtgggcgc tgagcgagga aaatcagctc gagctcacct tgcgcagcgc
     3601 gcctccacgg aaggttcgct ccgtcacgcc gagcgcaggc gcgggatccc gacctgccgc
     3661 ggtgcgctcc gaatttgccg aacttgcgca gctcggtgag caaatcgccg ccactcccgc
     3721 caagctggag aagattcgcc tcctctccga atacctgcgc gcgctcgacc cggagggact
     3781 ccccattgcc gccattttcc tgaccgggaa accatttgcg caaaccgacc agcggacgct
     3841 gcaaaccggt tgggcgatca tcttccgcgc gctcctcggc gcgagccggc ggggcgacgc
     3901 cgaattgcgc cggatcgcgc gcagccatgg cgacgcaagc aaaagcgcac gcgaagcgct
     3961 cgaggggcga acgacgcccg aacctttttc gttaggcgag gcgcacgccc ttttcgaagc
     4021 attgcacaag acccgcgggc cggccggcaa gaccgaactg ctccaggcgc ggttgcaaaa
     4081 actctccgcg ctcgaagcgc aatacgtcgt caagatcctg accggcgatc tgcgtatcgg
     4141 cttgcgcgag ggactggtcg aggaagcgat cgcgcgcgct ttcgatgccc cgcttgatga
     4201 tttgaaggaa gcccacatgt tgttaggcga catcggccag acagccctcc tcgcggcgcg
     4261 acgcgaattg gatcgcgcgg agctgactct cttccgtccg ctcaagtgca tgttggccag
     4321 cccggagccg accgcggaag cgatctgggc gcgcttcgcg gataagtccc aaacgaccta
     4381 tgtggaagac aagttcgacg gcatccgcgc ccaactgcac cgcggcgtgg atcgggtcga
     4441 gcttttctcg cgcgatttgc gccgggtgac ggggcaattc gaagagctcg ccgagggggc
     4501 gggtggcctg ggtgacgagg tcgtcctcga cggcgagatc atcgccttcg agcgcgggga
     4561 aaagctcacc tttttcgatc tgcaaaagcg gctcggacga aaaagcgaga gcgccgattt
     4621 atttgccgcg cccacggccg atgtgccggt tgttttcgtg gcgtttgact tgctctggtt
     4681 gaacggtcgt tcgctcctcc agacgccgtt gcgcgagcgc cgcgaactgt tgcgcggcct
     4741 aacgatgccg gcgcaatttc aaatcgcgga ggtttctccg gccggatccg ccgaggagat
     4801 cgagttggct ttccagctgg cgcggcggcg cctgaacgaa ggtttgatga tcaaggaccc
     4861 ggagagtccc tacacgcccg gccggcgtgg tctgtcgtgg ttcaagctga agaaagagct
     4921 ggccacgctc gatgtggtgg tggtcgccgc ggaactcggg cacggcaagc gcaaccatgt
     4981 tttgagcgat tacaccttcg cggtgcgcga cgaggagagc gggatgcttt tgccgattgg
     5041 caaggcttac agcggcctga cggatgtcga gattgcggaa ctgactgagc attttcgccg
     5101 gaacacgact agcgaccacg gccgctacct cgaggtaagg ccggacacgg tgctcgaaat
     5161 cgccttcgat tccatccagc cgagcacgcg gcacgcgagc gggctggcgt tgcgcttccc
     5221 ccggatcaag gcgatccgcc gcgataagac gccggacgca atcgacaccc tggcctacgc
     5281 gcgccagctg gcggacgcga ctcgttaggg ttggtcggcc acggcggtgc gagcggagga
     5341 caactcggcg gcggcctgag tcgcgttcgc ggcgaaacga ttttcttcag cgacgagcgc
     5401 ctgttcgcgg gcgtcgggct cagcctggcg ctgcgccagt tttttttgtt cgtcggcccg
     5461 gtgctcggcg ttaaggcgct cttgcacctg ccgttcaatt tcggcgtttc tttccgcttc
     5521 ggtcggtcgt ttcccgcagg aactaacgaa agccgcgagc gccgcgaaga ggggcaggag
     5581 caagattctt ctcacaaaac agtgagaccc ggtttggcgc aggttattcg aagtaaaccg
     5641 gccgggaagc gcgtcggcgg ctcgttagcg ctcgttaggc gaaggtgatg ctggagcgcc
     5701 cggcacggga atgatcgtga cggtttcaat tttgggccgt tggagacgct gccattgccc
     5761 gtagcctgcg agcagggcaa agacggtcag gatcgccagc atcagtttca atccgctcgc
     5821 cggcgcgcgg ctggtgccgg gggaacgagc gggtagtccg ggcaattcca tcggtcagta
     5881 tcgcggaccc tggcgcgggt cgcaaacccg gtcggcggat agactcgtgc tcgtgctcgt
     5941 aaaatcgtaa tcgatctctc cagtgaaaag gatcgagcac gagcacgagc acgagtcgcg
     6001 accgagcggc tcgggttatt ccaacttcag cgcggcgatc gcttccatca cccgcgcgct
     6061 gaggcggggg tagacttcgc gcccgatgcc cgcgaggtcg gccgccgtga agtgaagcgg
     6121 ctcgcccacc acgatcgtga tcggacaaaa acgcagtttc ccgccgccgc gcggcaaggc
     6181 ccgatgcgct ccgaaaatcc gcatcggcac gacgggcgcg agagtctttg caatcacgaa
     6241 accaagcccg ggctggggtg gctgcaattg gccgtcgagt gtgcgcgagc cctctggaaa
     6301 aacgagcgcg ccgtgattca cgcgcagggt tcggatgagc gctttcaacg cgctgcgatc
     6361 gccgccctcc tggtcgactg ggatgacatt cagtttcggc agcacccaac cgagcacggg
     6421 gacatcgagc aacgatttgc gcgccagaaa atagatcgcg cgccggcagg cgatgccggc
     6481 gaagggcgga tccaaatagc tttcgtgatt cattgccagg atgaccggcc cttcctggat
     6541 gacgcgctcc ggatgaatga cgcgaaagcg aaaaaggacc cgcgcgatca ggcgggtgag
     6601 gtgatagatc gtgaaatacc agggattcac cgcgcgatgg ttagtccctt tagtttcaag
     6661 cggccgatga tttcgccgac cactccatcg atcgtcaggt tggagctgtc gattacatgc
     6721 gcgtcctcgg cgatgatcaa aggggaggcg cgacgcgacg aatccgcttc gtcccgcgcg
     6781 gtaacttggt cgcgttgtcc ctgcgccgcg cgtcggcgca agcgaatttc cggcgaggcg
     6841 tcgatgtaaa acttgaaggg cgtctcggga aaaacgaccg agccgatatc gcggccttcc
     6901 atcacgagat cgttcgtcac ggcgtaacga cggagatgat cgaccaggat ttcccgcaca
     6961 cgcggaacgc tgctgacgag cgaaacattg tcattcacgc gatcttcgcg caggaacggc
     7021 tcgggatcga cgccgtcgat ccgcacgaac gagtcgcggt cgcgcagctc gcaggcaatc
     7081 gcggcgctcc cggcgagctg ctcgatcgcc gccgcatcgt cggcggggat tccgcgctcg
     7141 agcacgagcc aggttatcgc ccggtacatc gcgccggagt tgacgtaaac aaagccaagg
     7201 cggcgcgcca gctcccgggc gacactgctt ttcccggaag cggccgggcc gtcgatcgcg
     7261 atgatcttgt gcgcggaagg ggcgttcatc gcgagaccgg cgcgcggggg cggctatacc
     7321 tcctccgcgc gcgagtgcga gagtgaactg atgaccggtg tggtggtgcg aaaccccttc
     7381 ggattaccca actcgctgag catcttctca aaacccggat aggaactcgc gacacaatct
     7441 acgtcctgga tgaccgtctc gccttccgcg aacatacccg cgacggcgaa cgccatcgcg
     7501 atgcggtggt cgccgaaact cgaaagccgc gtgccatgga gcggagtgcc accgtggatc
     7561 tcgagtccgt cggtcaactc gaccacttgt cctcccattg agcgcaggtt gtgggcgatg
     7621 gcggcgatcc ggtcggtttc cttgacgcgc aattcctggg cgtgccggat gatggtagtt
     7681 ccgttggcga gcgcgccggc gacggcgaga atcggcaact catcgatcag ttgcgggatt
     7741 tctttaccct ggatgacggt cgcctttaaa cgcgcgccgg tgatttcgac gatgccgcgc
     7801 ggctcgacct gatcgacgtc ctcgatcgct tcgcgcacct gcgcacccat gcgcacgagg
     7861 acaccgagca agccggtgcg ggtttcgttc aggccaactt cccgcaccag gagatgaccg
     7921 cccggttggg ccgccgcggc gacgagccag aaggcggcgg aagaaatatc gcccgggatg
     7981 ctaaagtcgc gggactccgg cacgtgatcg ccgaagacac tcacgccggt ttcatccttg
     8041 tgcgtgcgga tgagaaagta attgagcatc agctccgtgt ggttgcgcgt ggggacgggc
     8101 tcttgcacgg tggttttccc cttggcaaag aggccggcga aaagcagggc gctcttcaac
     8161 tgcgcgcttg ccaccggcat cgaatacttg atcccatgga gcggcgcgcc ctcgatccgc
     8221 agcggcgcgg tttcgtgttc gccctcggct gcgattttgg cccccatcaa acggagcggc
     8281 tcaaggatgc gcgccatcgg gcgttccgag agcgagccat cgccgacgag ccggctcttg
     8341 aacggctggc cggccagcat gccggccagc agtcgcattg tagtgccgga gttgccgcaa
     8401 ttaatatcgg cttgcggggc ggttagtttc cgtttcttgc cgtgcacgat gagcacttca
     8461 ggctcgggtt gttcgatcgt gatcccgagc gcacgcatcg cgacgacggt gttcatgcaa
     8521 tcggcgctgc gcgaaaagcc gcgcaacacg cacgggccgt tggccagcga ggcgatcagg
     8581 acggcgcgat gcgaaatact tttgtcgccc gagacggtga tctccgcctc aattcgcggc
     8641 gcgcgtttta tgcgaaggtc atccatgttt ttgggaaaaa ggcgccggtc gcgggcttac
     8701 cggccagcgt ggagaagaat cccaagttgc gtgtaaatgt ccagtgcggc ggcgcgaaaa
     8761 cggcgcaccg ccggtccgcg gagggtgcga cacggggacc gcggttcgtc agggaacgag
     8821 gaagattttt tgcgtgtagg gatcgcgcac ctcggagccg ggtgggaaac cccgcacgtc
     8881 gaccagcccg gcgtcgggtg cgtaagggct cttgacaaag cccggcttgt cgggcaccgg
     8941 aatgccgtaa ggtacgtcgc ccttcgtcgg cttgaccggc gcggccgtgg ccgcggtctg
     9001 cggcggcggc ggcggggttg tagtgacttc cggtggcggc ggcgtgcctt gattgaaggg
     9061 ttgctgggtc gggtattgcg tctcgggcgc ttgctggtag gtggtggcat gcggacggcg
     9121 cggcggcggc tccggttccg aacaagcggc aacgaaaagg gtcgtgagga gcgcgagcga
     9181 gatcgatttt ttcatacggg ttttgccttg agcgatgtga ccagtttgta gtgcattcgg
     9241 tcgcgagtga caaagaatat ttgcagcaga tcgcgcggcc cattcgtttc cggacaaaaa
     9301 tacaaattca cgggcgatgc ttttcgaatc aaaaaaggaa atggagcggc gcgaagaagt
     9361 cattctggtg attgacgatg accgtgggag tcggcggatg ttgctgcggg cgctgggcga
     9421 agccggctat gcctgccgcg aatcggccag tggggttgag gcgctggaga tgctccacgc
     9481 agaggcgccg tcgctgctcc tgctcgattt cgatatgccg gaactgaacg gggcggaggt
     9541 gctttcccgc ctccgcgagg atgccgaccc cgcgattgcg caattgccgg ccatcatgct
     9601 caccggcttc ggcggcaggg caaccgaagt gcattgtctg gaggcgggcg cgaatgattt
     9661 cgtcaccaag ccgatcaacc tcccggtcct gcgggcgcgg atcgatacgc agttgcggct
     9721 gcaatcgctc cgtcggcagt tgcagcagca gaacgatgag ctggaggcgt ggcgcaaaga
     9781 tctggagcga gacctggcag cggcccgtct tacccagcga tcgctcatcc cgcagaagcc
     9841 accgcttttg cccggctggg aagtggccgc ggtctatcgc ccggtcattc aggtcggagg
     9901 cgatatctac ggctggttgc ggatgacgga tggtcgcacc ctcttctgga tcgcggatgc
     9961 gaccggccat ggggcggcgg cggccttgct caccaccctc gcgaagttgc ttttccacca
    10021 cggcagcggg gagcacgcgc gcccggcgga aatcatggag gcggtgaaca acgatttccg
    10081 cagtattttc ggcgcgcgct ccttcatgac ggcgatgtgc gtcgcgctcg aggcgcagac
    10141 ggggtgcgcg agtgtcgtcg gagcgggcca tccgccgctc attgttgcgc gccgcgcggg
    10201 ggcggccgaa ctggtcactt cgatcgcccc cccgttaggc ttgctcgaga agtcgcactt
    10261 cgaggaaacg accattgccc tgcaaccggg cgacgggttc cttctctaca ccgatggcct
    10321 ttacggcgca tcgaaagtgg cagccgagcg ctggacgccc acgcgtttgc aggcgctgat
    10381 gaccgacgat cactcgagcg cgcaggcttt gctcgggcat ttactcgagc gggctgcgcc
    10441 ggataagggg gagccacagg ctgacgatct ggcggcgctg gtcgtgcggc gcgggtcctg
    10501 agcccgtaat attttttttc gccggagtaa aattagtatt gctctcgtgc agcccaacga
    10561 ttaatcagcc tctatgccca aatccacagc caaaaaaacg ccctcgaaac gcaaacccaa
    10621 cgcagctttt atgaagcccg ttcagcc
//
LOCUS       DATMNS010000239         8306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2497956, whole genome shotgun sequence.
ACCESSION   DATMNS010000239 DATMNS010000000
VERSION     DATMNS010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2497956"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            277..2037
                     /locus_tag="VNW28_08420"
     CDS             277..2037
                     /locus_tag="VNW28_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA09992.1"
                     /translation="MNTVWRVFAYLKRYPWMAAGTLGCAIVSTLMIIVFPAVTKFIID
                     DVIAGHRPALLLPMVGLALAAFFLQDVLNGLRILLNNNFEQKVIFDLRSDLFRHIERL
                     PLRWFDNRATGDLITRVVEDVNSVERVLIDGIEQGVVAILQVAIVSVALFYFNGSLAW
                     CVLAPVPLLAGGALAYTLTGPSRYRPQRRAASALNALLLDDLAGIRQIKSFVREKQEH
                     LRFNRASETLRQATLRIMRAWALYSPSMDFLNSLGLILAIGFGGRSVLRGEMQVGSLV
                     AFITLTRFLYEPVTRLHQLNQLVQAGRAAGERVFEILDEPEEPGLREGETVTEIEGEI
                     RFGDVSFAYAKGLPALSNISFHARPGETIALVGATGAGKTTLVNLLARFYEYTSGAIT
                     IDGKPIREFGLHALREMIGMVTQESFLFNGTIRENLLLGKPDATDTELLEAAEAANAR
                     AFIDRLPTGLGSVVGERGVKLSVGEKQRLSIARALLKDPPILILDEATASVDTTTERL
                     IQQALENLMFHRTCIVIAHRLSTIVRADQILVLDHGRIIERGTHAGLLSLGGKYARLC
                     EQSFLETTGEIDAAPEFVAN"
     gene            complement(2124..2585)
                     /locus_tag="VNW28_08425"
     CDS             complement(2124..2585)
                     /locus_tag="VNW28_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HXA09993.1"
                     /translation="MIRFRRTLALLCLSGFLAFHAPAQSPSPAAETSATSELLSLTRK
                     IDAQNVKIDALSQQILRLQQEIERPKATAAGEASSPVPAPAVGGSTHVVARGETLTSI
                     AKLHKVGIDELQKFNHIENDRKLQIGQTLVIPGAPGVAAPSPSPSATPPNE"
     gene            2665..2841
                     /locus_tag="VNW28_08430"
     CDS             2665..2841
                     /locus_tag="VNW28_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like protein UBact"
                     /protein_id="HXA09994.1"
                     /translation="MPERTEKPVAPVPWGPQQGDGGGPKSPDVSKPDTRDLLKKMRKV
                     DPNQSKRYRQRTGQ"
     gene            2838..3641
                     /locus_tag="VNW28_08435"
     CDS             2838..3641
                     /locus_tag="VNW28_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome subunit alpha"
                     /protein_id="HXA09995.1"
                     /translation="MSDLLGAAAFDFPALLRRHGLLNEASLLPPASTSHPASTFTPTQ
                     ATTILAFKFTGGVLVAGDRRATAGNTVVYDRADKVLEIDRHSIMAIAGVPATAWEMAR
                     VLEHSLQYFRRTQLQEMSVDGKVRALSKLLRDNFGFVLQGVGVVVPIFATYDFAGDGQ
                     ARLYFYDAMGAQFEVAEYAATGSGSPAVRGVLFYENRWGKKRLQKLSEEDAVTVALRA
                     LDTAAEADTATGGVDRHAKIFPLIKIISHAGIKTLPEEQIAARFKRTVA"
     gene            3638..4417
                     /locus_tag="VNW28_08440"
     CDS             3638..4417
                     /locus_tag="VNW28_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA09996.1"
                     /translation="MIEEPYRWVEAIANRREYIETQLAPGSPIAALSYRDGILFVTVS
                     RARQKLFEIYDRIALGAIGHPGDIERLRMAAIELASTEGFTRSAGDVSLRRLAFYSLS
                     PVLKTAFEQVYGAPYLARMIFAEVGASPAEDLFLRVEYDGAIAAGGASADTQPPRFSA
                     ISGTTPSAELMEELLRRDHDSDCTLGAALKVALDAWSAGSMRAGDDEVKEIPGHAALL
                     KHRQEKLKSATVEACVLERSGANAIRYRSIADGELKELTAA"
     gene            4596..5363
                     /locus_tag="VNW28_08445"
     CDS             4596..5363
                     /locus_tag="VNW28_08445"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXA09997.1"
                     /translation="MFPWLGVLLLTVSFVWFVRLPVPMHPASAPRQPRVRGAQPLVVV
                     LDPGHGGQDSGAICGGLAEKDLALDVALRTELLLRADGCATVLTRANDRYLSLAERAA
                     VGNREENSLFVSIHFNDGTRATASGVETYYAPQQSASHFGFLSWLPFLQRTEASPLTG
                     KSESLASCIQAALIARTGAVNRGIKAEQFYVIANVRHPAALVEGGFLTNKSDVTRLAT
                     AEYRQQIAAAISDGVHRYREAIRQGEPTLALAAAQPE"
     gene            5393..6115
                     /locus_tag="VNW28_08450"
     CDS             5393..6115
                     /locus_tag="VNW28_08450"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HXA09998.1"
                     /translation="MQRIVFAFLALLVVGAGALRADQTTSAVQQALKDQGFYYGDVTG
                     EKTADTTAAIRRYQIRNGLQITGEIDAETLRSLGVGGAGARAAAQPRATTPPEEETEA
                     NSGDTATQSAEISPPPPPAGYAPQGTGGLRPEASGVFQGTPYEIAPPDLQRHVIVGAQ
                     TLLARYGYYRSGIDGEFGPGTAVAVRTFQSRAGLVPDGRLNMETLGALGLLPGQHAPG
                     FSVPRRRLQGRPIYRGQWIPGT"
     gene            complement(6212..7015)
                     /locus_tag="VNW28_08455"
     CDS             complement(6212..7015)
                     /locus_tag="VNW28_08455"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HXA09999.1"
                     /translation="MGFRGIIAIWLSASLASISPTATPPAWEKTVTSLPRGDFPDPRP
                     FVATYNFGWNDLVAATADVQFDKSPTRLQFQGVGQTVGVARALWKFDVRHRAVTDAIT
                     LRPIEMSQVEERSRKTVTDDLVFTADGVERTRIDTKTNKRSELKTFTFPGGVFDMHSA
                     LLYLRSQPLRESDVYRLVVYPATSPYLATLTVSGHPSITIGAGTYKAIKLDVQLYKIG
                     KNGELEPHKKFRRASVWVSDDADRLLLRVEAGIFIGTVFAELQSVRFRL"
     gene            complement(7186..7791)
                     /locus_tag="VNW28_08460"
     CDS             complement(7186..7791)
                     /locus_tag="VNW28_08460"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXA10000.1"
                     /translation="MKKLILVAVLSAFALPVGAFAQGMKVGTIDMNRAFKEYNKTKDA
                     ETKINDAKNAAKKEYDDRADAYKKALDEINKLNQQLEAPALSAEAKTQKAKDRDEKIA
                     SIKNMEREINDFRQTREKQLQEQAVRMRDGIVKEITDVVMAKVKADNMDLVFDSSGVS
                     LNGVPMLMFSKPEMDFTDSVVAQLNKPAPKAAATPAAKAKP"
     gene            complement(7850..>8306)
                     /locus_tag="VNW28_08465"
     CDS             complement(7850..>8306)
                     /locus_tag="VNW28_08465"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXA10001.1"
                     /translation="EASQYFLFPYDIILTLNGETAVIDNWGSGDHVAIFDRLFLGGAN
                     DLRGFNFRDVGPKDMNGEPLGGNTLARFTIETTIKVIDRVRAAVFYDTGFVNVNAYDF
                     GTSHLASDVGFGLRLDLPIGPLKIDYGIPIQKDNNSGGGKVQFSVGYQF"
BASE COUNT         1506 a         2382 c         2761 g         1657 t
ORIGIN      
        1 cgccgcgaat gccgcggccg gcgacgcact cgatctcggt cgtctcgacg aggggaaaat
       61 catccggcgg ctggccgtgg tggccgtagt agttgtggcc gggagaggta aagatttggt
      121 cgatgatcat gcggaagggg atcctggaaa acgttcaacg ttctacgttg aacgttcaac
      181 tcgcgagagc tttcggagtg caacgtccaa tcggggagca actcgttttg cgttgaacgt
      241 tggacgttgg gcgtcggacg ttggaccgtt ccgcacatga acaccgtctg gcgcgtcttc
      301 gcctacctca aacgctatcc ctggatggcc gccgggacgc tggggtgcgc gatcgtgagc
      361 acgctcatga tcattgtctt tccggcggtg acgaagttca tcatcgacga cgtcatcgcg
      421 ggacatcggc ccgccttgct gctcccgatg gtcggtctcg cgctggccgc ctttttcctg
      481 caggatgtgc tcaacggcct gcggattttg ctcaacaaca acttcgagca aaaggtcatc
      541 ttcgatctgc ggagcgatct tttccggcac atcgaacggc tgccgctgcg ctggttcgat
      601 aatcgcgcga cgggcgacct gatcacgcgc gtggtggagg acgtgaactc ggtcgagcgg
      661 gtcctgatcg acgggatcga acagggtgtc gtcgcgattt tgcaggtcgc gatcgtgagc
      721 gtcgcgctct tttactttaa cggcagcctc gcctggtgtg tgctcgcgcc ggtgccgttg
      781 ctcgccggcg gcgcgctcgc ttacacgctc accggcccga gccgctatcg tccgcaacga
      841 cgcgccgcct cggccctgaa cgcgctcctg ctcgacgacc tcgccgggat tcggcagatc
      901 aaatcgttcg ttagggaaaa acaggaacac ctccggttta atcgggccag tgaaaccctg
      961 cgccaggcca ccctgcggat catgcgcgcc tgggcgctct acagtccgtc gatggatttt
     1021 ttgaattcgt taggcttgat tctcgcgatc ggcttcgggg gacgctcggt cttgcggggc
     1081 gagatgcagg tcggctccct cgtcgccttc attaccctca cgcgattcct ctatgaaccg
     1141 gtgacgcggt tgcaccagtt gaatcaactc gtgcaagccg gccgcgcggc cggcgagcgc
     1201 gttttcgaaa ttctcgacga accggaagag ccggggctgc gcgaaggcga gacggtaacg
     1261 gagattgagg gcgaaattcg cttcggcgac gtaagcttcg cctatgcgaa aggcctgccg
     1321 gccttgagca atatttcatt tcacgcgcga ccgggcgaaa cgatcgcgct ggtcggcgca
     1381 accggtgcgg gcaaaacgac gctcgtgaat ctgctcgcgc gcttttacga atacacctcc
     1441 ggcgcgatca cgatcgacgg aaaaccgatt cgcgaatttg gtctgcacgc gctgcgggaa
     1501 atgatcggga tggtgacgca ggagagcttt ctttttaacg ggacgatccg cgaaaacctt
     1561 ctcctcggga agccggacgc gacggacacc gagctgctgg aagcggccga ggcggcgaac
     1621 gcacgcgctt tcatcgaccg gctgccgacc ggcctcggca gcgtggtcgg cgaacgcggg
     1681 gtgaaactga gcgtcgggga aaaacagcgg ctttcgatcg cgcgcgcact gttgaaggat
     1741 ccgccgatcc tgattctcga cgaagccacc gccagtgtcg ataccacgac cgagcgcctg
     1801 atccagcagg cgttggagaa cctgatgttt catcgcacct gcatcgtgat cgcgcaccgg
     1861 ctctcgacca ttgtgcgggc ggaccagatc ctggttttgg accacgggcg tatcatcgag
     1921 cgcgggacgc atgccggact gctatcgtta ggcggaaaat atgcgcgcct ctgcgagcag
     1981 agctttctgg agacgaccgg cgagatcgac gccgcgccgg aattcgtggc gaactgagca
     2041 acgcccttcc cggggcgagc gcgacgacgc taacttccaa ttcgagctgg gggccgaaaa
     2101 atgctaattc gccgccgagg ccatcactcg ttaggcggag tggcgctggg ggaaggggag
     2161 ggcgcggcga cgccgggcgc accgggaatc accagggttt gtccgatctg cagcttccgg
     2221 tcgttctcga tgtgattgaa tttctgcagt tcgtcgatgc cgactttgtg cagcttcgcg
     2281 atcgaggtca gcgtttcgcc gcgagcgacg acgtgggtgg atccacccac ggcgggcgcg
     2341 gggaccggcg acgacgcttc gcccgccgcc gtcgctttgg gacgctcgat ctcctgctgc
     2401 aaacggagga tttgctgcga gagagcgtcg atcttgacgt tttgcgcgtc gatctttctg
     2461 gtcaggctga ggagttcgct cgtggccgaa gtttccgcgg cgggactggg actttgggcg
     2521 ggcgcgtgga acgcgaggaa gccgctcaaa caaaggagcg caagcgtgcg gcggaagcga
     2581 atcatgggca agcttgacat tttcgtcgga tgctggcaag cgcgccagtt tgcgttcgtg
     2641 acgcattaac ggtagcgttt ttccatgcca gagcggacgg aaaaaccggt ggcgcccgtg
     2701 ccttggggac cgcagcaggg tgacggcggc gggcccaagt cccccgacgt ttccaagccg
     2761 gacacgcgcg atttgttgaa gaaaatgcgc aaggtggacc cgaaccaatc gaagcgctac
     2821 cggcagcgca ctggccagtg agcgacctat taggcgcggc ggcgtttgat tttccggcgc
     2881 tcttgcggcg gcacggcttg ctgaatgaag cgtcgctcct cccgccggcg agcacgagtc
     2941 acccggcatc aactttcacg ccgacgcagg cgaccacgat cctcgccttt aagtttaccg
     3001 gcggcgtttt ggtcgccggc gaccggcggg cgacggccgg caacacggtg gtctacgatc
     3061 gcgccgacaa ggtgttggag atcgaccggc actcgatcat ggcgatcgcg ggcgtgccgg
     3121 cgacggcctg ggaaatggcg cgtgtgctcg agcattcgct ccaatatttt cgccggactc
     3181 aacttcagga gatgagcgtg gatggcaagg ttcgcgcgct ctcgaaactg ctgcgggata
     3241 attttggctt cgttctgcag ggcgtcggcg tcgtcgtgcc gatttttgcg acctacgatt
     3301 ttgcgggcga cggtcaggcg cggctttatt tctacgacgc gatgggcgcg cagttcgagg
     3361 tcgcggagta cgcggcaacc ggctcgggtt cgccggcggt gcgcggggtc ttgttttacg
     3421 aaaaccgctg gggaaaaaaa cggttgcaga agctcagcga agaagatgct gtgaccgtcg
     3481 ccctgcgggc cctcgacacg gcagcggagg ccgacacggc gaccggtgga gtggatcgtc
     3541 atgcgaagat cttcccgctg atcaaaatca tctcgcacgc cgggatcaaa acattgccgg
     3601 aggagcagat cgccgcgcgt ttcaagcgca ccgtcgcatg atcgaggagc cctatcgttg
     3661 ggtcgaggcg atcgcgaacc ggcgcgaata catcgagaca cagctcgcgc cgggcagtcc
     3721 gatcgcggcg ctgagttatc gcgatgggat tctcttcgtg accgtcagtc gggcgcggca
     3781 aaaactcttc gagatttacg atcgcatcgc gctcggggcg atcgggcatc cgggggacat
     3841 cgagcggctg cggatggcgg cgatcgagct ggcgagcacg gagggcttca cccgttcggc
     3901 gggcgatgtg tcgctgcgac ggctggcttt ttattcgctc agcccggtct tgaaaactgc
     3961 cttcgaacaa gtctatggcg cgccctatct ggcgcggatg atttttgcgg aagtgggcgc
     4021 gtcgccggcg gaggatctat ttttgcgagt ggaatacgac ggtgcgatcg cggccggcgg
     4081 agcgtccgcg gacacccagc caccgcgctt tagcgccatt agcgggacaa cgccttcggc
     4141 cgagttgatg gaggaactgt tgcgtcgcga ccacgattcc gactgcactt tgggcgcggc
     4201 tctaaaagtg gcgctcgacg cgtggagcgc gggcagtatg cgggcgggcg acgacgaagt
     4261 gaaagagatc ccggggcacg ccgcgctgct gaagcatcgc caggaaaagc tgaagagcgc
     4321 gacggtagag gcctgtgttt tggagcgaag cggcgcgaac gcgattcggt atcgttcaat
     4381 cgccgacggg gagttaaagg agctgactgc tgcctaacga attagtaatg agtgattcca
     4441 tcggcgtcgc cgtggcgttg tagccggggg cggtgacccg accttttttg gcgataaacg
     4501 ctctgcgatg ccgggggggc gacgcccccg gctgcgaccg cgtgcctcgc gcttgcttgc
     4561 gaagcagcgg ttgagtaaga ggaaatatgc ggcgcgtgtt cccgtggctc ggggtccttc
     4621 tcctgacggt ttccttcgtc tggttcgtcc gactaccggt gccgatgcat ccggcgtccg
     4681 cgccgcgcca acccagggtg cgcggcgcgc aaccgctggt cgtggtgctg gatcctggac
     4741 acggcggaca ggattcgggc gcgatctgcg gggggctcgc ggaaaaggac ctggcgctcg
     4801 acgttgctct gcgcacggag ttgctcttgc gtgcggacgg ttgcgcgact gtcttgacgc
     4861 gcgccaacga tcgctatctg tcgcttgcgg aacgggccgc agtcggaaat cgcgaggaga
     4921 actcgctttt cgtcagcatc catttcaacg atggaacacg cgcgacggct tccggggtgg
     4981 agacctatta tgctccgcag cagtcggcgt cgcactttgg ctttctctcc tggctgccgt
     5041 tcttgcaacg aaccgaggcc agcccgctga cgggaaaaag cgaaagcctg gccagctgca
     5101 ttcaggcggc tctgatcgcg cgcaccggcg cggtcaatcg cgggatcaag gcggagcagt
     5161 tttacgtcat cgccaacgtg cggcatccgg ccgccttggt ggaaggcggc ttcttgacca
     5221 acaaatctga cgtgacgaga ttggcgaccg cggaatatcg tcagcagatt gccgcggcga
     5281 tcagtgacgg cgtgcatcgc taccgggaag cgatccggca gggcgagccg acgctggcgc
     5341 tggccgccgc ccagcctgaa taacgggaaa cataccgcgt cgaatcccac ctatgcaacg
     5401 aatcgtcttc gccttcctcg ctcttctggt ggtcggcgcc ggcgcgctgc gggcggatca
     5461 aacgacctcg gccgtgcaac aggcgctgaa ggaccaggga ttctattacg gagacgtgac
     5521 cggggagaag acggcggaca cgacggcggc gattcggcgc taccagatcc ggaacgggct
     5581 ccaaatcacg ggagaaatcg atgctgaaac gttgcgatcg ttaggggtgg gcggcgcggg
     5641 agcgcgggcc gcggcgcagc cgcgcgccac gacgccgcca gaagaagaga cggaggcgaa
     5701 cagtggagac acggcgacgc agtccgcgga gatttcgccg ccgccaccgc cggccggtta
     5761 cgcaccgcag ggaacaggcg gattgcgtcc tgaagcgagc ggtgttttcc aggggacgcc
     5821 gtacgaaatt gcgccgccgg atttgcaacg gcacgtgatt gtgggggcgc agacgctcct
     5881 cgcccgctac ggctattatc ggagcgggat cgatggtgag ttcggtcccg gcacggcggt
     5941 ggcggtgcgg acattccagt cgcgcgccgg gctcgtgccg gacggtcggt taaacatgga
     6001 gacgcttgga gcgctcggtt tactcccggg gcaacacgcc ccgggatttt ccgttccgag
     6061 acggcggctg caagggcggc cgatctatcg cggacaatgg attcccggta cttagtcgtg
     6121 atgcgccaac gctcaaagct gtagacgagg gcgccgaccc tggcttcgcc aagcttggtc
     6181 ttcttacccg ccgggattca gcaactccag gctacaatcg gaaacggacc gactggagtt
     6241 cggcaaagac cgtgccgata aaaatgcccg cctcgacccg gagcaagagt cgatcggcgt
     6301 cgtcggaaac ccacacgctc gcgcgccgga atttcttgtg cggctcgagc tccccgtttt
     6361 ttccgatctt gtagagttgc acgtcgagtt tgatcgcttt gtacgtgccg gcgccgatcg
     6421 tgatggaggg atggcccgag acggtgagcg tggcgaggta ggggctggtt gcgggataga
     6481 ccacgagacg atacacatcg ctctcccgca ggggctggct gcggagatag aggagcgcgg
     6541 agtgcatgtc gaagactccg cccggaaaag tgaacgtttt cagttccgac cttttgttcg
     6601 tcttggtatc gatccgggtg cgctccactc cgtcggccgt gaagaccaga tcgtcggtca
     6661 cggtttttcg gcttcgctct tcgacctggc tcatctcaat cggtcggagg gtgatcgcat
     6721 cggtcaccgc gcgatggcgc acatcaaatt tccagagtgc gcgggcaacg ccaacggtct
     6781 ggccgacgcc ttggaattgc aggcgagtgg ggctcttgtc gaattggacg tcggcggtgg
     6841 cggcgaccag atcgttccag ccgaagttgt aggttgcgac gaatggtcga ggatcgggga
     6901 aatctccgcg cggcaagctg gtgacagtct tctcccaggc cggaggagtg gcggtcggcg
     6961 aaatactggc caggctcgcg cttaaccaga tcgcgataat cccacggaaa cccaccggtc
     7021 gaccatccga tgccggttgg cgacttgcaa atggctgtcg ccggggtcgc gccctggcat
     7081 tctggtgatg gcgcgagcgc ttctgggatt accgactcca gcgatcaggg cgaaaaaaaa
     7141 gcgcaggccg tcggcgtgcg cttttccttg aaattgattc gctcgttagg gcttcgcctt
     7201 ggcggcggga gtggcggccg ctttcggcgc tggtttgttc aactgcgcga cgaccgaatc
     7261 ggtaaaatcc atctcgggct tggaaaacat gagcatcggg acgccgttca ggctcacgcc
     7321 tgaggagtcg aagacgagat ccatgttgtc ggctttaacc ttggccatga cgacatcggt
     7381 gatttccttc acgatcccgt cgcgcatccg cacggcctgt tcctgcaact gcttctcgcg
     7441 ggtctggcgg aaatcgttga tctcccgctc catgttcttg atgctggcga tcttttcatc
     7501 gcgatctttc gctttttgcg tcttcgcctc ggcgctcagg gccggggctt cgagttgctg
     7561 gttgagtttg ttgatttcgt ccagcgcctt cttgtaggca tcggcgcgat cgtcatattc
     7621 ctttttcgcg gcgttcttgg cgtcgttgat tttcgtctcg gcgtccttcg ttttattata
     7681 ttctttgaag gcacgattca tgtcgatggt gccgaccttc ataccttgcg cgaaggctcc
     7741 aaccgggagc gcgaaagcag acagaacggc cacgaggata agctttttca tagatgtagg
     7801 gtgagacagg taattgggat ggcgttagac tgcaagtccc accttttgtt caaaattggt
     7861 agccgacgct gaactggact ttgccgccgc ccgagttgtt gtccttttgg atggggatgc
     7921 cgtagtcgat cttcaacggc ccgattggga gatcgaggcg caagccgaag ccgacgtcgg
     7981 aagcgaggtg cgaggtgccg aagtcgtagg cattgacgtt gacaaagccc gtatcgtaga
     8041 aaacggcggc gcgcacgcgg tcgatgacct tgatcgtggt ttcaatcgta aagcgggcga
     8101 gtgtgttgcc gccgagcggt tcgccattca tgtctttcgg cccgacgtcg cggaagttga
     8161 agccccggag gtcattcgcg ccaccgagga acaagcggtc gaaaatagcg acgtgatccc
     8221 cgctccccca gttgtcgatg accgcggttt ctccattgag cgtgagaatg atgtcgtaag
     8281 gaaaaaggaa atattgcgag gcttcc
//
LOCUS       DATMNS010000240         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4113022, whole genome shotgun sequence.
ACCESSION   DATMNS010000240 DATMNS010000000
VERSION     DATMNS010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4113022"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="VNW28_08470"
     CDS             complement(<1..366)
                     /locus_tag="VNW28_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HXA10002.1"
                     /translation="MALAMIAPPLWSQEDRKEADRLENCGTVIKEIMDIPDDIPQDLI
                     DKAECLIVYPSVLKAAFVVGGSYGRGSMTCRTGEHYTGPWSAPSMMALEGGSIGFQIG
                     GQATDFVLLVMNPRGAHSML"
     gene            644..2236
                     /gene="dacB"
                     /locus_tag="VNW28_08475"
     CDS             644..2236
                     /gene="dacB"
                     /locus_tag="VNW28_08475"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HXA10003.1"
                     /translation="MALSGLPVAPGGAQSNPVREPACSATKPDGGREERSPKRAAAAA
                     ARFAGRAATLLAASPASKGDWGLLIVDAETGETLFERNADGYFVPASNMKLFTTALAL
                     ARLGPDYKFHTTLETRGTISPDGTLSGDVLLVGRGDPNLSNRRFPFELKEEFDGPPEK
                     ALIELAEALRAGGVKEISGDVIGDDSYFPRERYPNGWEIDDMVWEYGAAISSIVVDDN
                     TVALTLTPGERMGDPVLASVAPLTPDFALENDVTTSAAEVKSDLTLTREPGANLVVVR
                     GTLPAKGAPRKLVLAIEEPALHAASLLAGLLSERGVKLDGKIRAVHVADTDRTPRTIL
                     AEHVSVPLGDSVKLVNKISQNLHTEMLLRTAVRQNGLWSTPEEMLKTPQDFYAAAGIA
                     PDDVMQTDGSGLSRHDLVTPRAIATLLRYAQGQTWFAPYYRSLPVAGVDGSLQDRMKA
                     TVAAGRIHAKTGSVEHVRTLSGFAETPSGRRLIFSFLGNNQGGGNHEAADVLNGLCVA
                     MIEEFDEAAAAIVPAHERKKQR"
     gene            2885..>3757
                     /locus_tag="VNW28_08480"
     CDS             2885..>3757
                     /locus_tag="VNW28_08480"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10004.1"
                     /translation="MRAESRRSVPGIMHGASATGQTVFLEPFETVEVNNQLVQLAEDE
                     AAEILRILRELTERLQVVREPLLVAAETIAELDGVFARGRFARDFDASMPEFSDASEL
                     RLVEARHPVLEDKLRREERAVVPLTLALGGAEKVLVISGPNTGGKTVALKTTGLAALA
                     AQSAIPVAAQRAVLPVFDRVLVDIGDEQSIAADLSTFSAHMLNLKTMLGLATPASLAL
                     VDEMGTGTAPEEGAALAVALLDEFRAKNCIVLATTHHDRLKTYASTTPGVVNAAVEFD
                     DVNLRPTYRLMVGVP"
BASE COUNT          715 a         1076 c         1248 g          718 t
ORIGIN      
        1 gagcatggag tgagctcccc gcggattcat caccagcagc acgaaatcgg tggcttgccc
       61 gccgatctgg aagccgatgc tcccgccttc cagagccatc atcgagggcg cgctccacgg
      121 gccggtgtag tgttcaccgg tgcggcaagt catggagccg cggccatagc tgccgcccac
      181 gacaaaggcg gcctttaaaa ccgagggata aacgatgagg cattcggctt tgtcgatgag
      241 atcctgcggg atgtcatcgg ggatatccat aatctccttg atcaccgtgc cgcagttttc
      301 aagccggtcg gcttcttttc tgtcttcttg ggaccacagc ggcggcgcga tcatcgccaa
      361 cgccatgaga caggaactga ttcgcttagt caaatttcac ctcgctgaag gttactgggc
      421 taagtaatga taggttgctc cggctttagg cgcaacctgc agtttgtcga ggaccgcggg
      481 tgctgccggt ttcaggcccg cgtgacctgt aggtccaggc gcgctgttca gaaaatgttg
      541 cgcgggcttt cgactatact ttcacctacg gcggtttatc ggcagaggat acatgggctt
      601 aggaaaccgt tttgcatggt gggccttggc gctgacagcg gcgatggcct tatcgggatt
      661 gcccgtggcg cccgggggcg cgcagagcaa tccggtgcga gaaccggctt gctcggcgac
      721 caagcccgac ggaggacgcg aagagcgttc tcccaagcgg gctgccgcgg ccgccgcgcg
      781 ttttgccggg cgggccgcga cgctgctggc cgcctcgcct gcgagcaaag gcgattgggg
      841 cctgctgatc gtagatgccg agaccggaga aaccctcttt gagcgaaacg ccgacggcta
      901 ttttgttcct gcttcgaaca tgaagctttt taccaccgcc ctggcgttag ccaggctggg
      961 tccggactac aaatttcaca ccaccctcga aacgcgcggc acgatctcgc cggacggcac
     1021 gctttctggc gatgtcctac tggtcgggcg aggcgacccg aatctctcga accgcagatt
     1081 tcctttcgag ctgaaggaag agtttgacgg gccgccggaa aaagcgctga tcgaactggc
     1141 cgaggcgctt cgggcgggcg gcgtgaaaga aatttcgggc gacgtgatcg gcgacgacag
     1201 ctattttccc cgcgagcgct atccgaacgg ctgggaaatc gacgacatgg tctgggagta
     1261 cggcgccgct atatcctcga tcgttgtgga tgacaacacc gtggcgctga cgctgacgcc
     1321 tggcgaaaga atgggagatc cggtgctcgc atcggtcgcg ccgctgacgc ccgattttgc
     1381 tttagagaac gacgtgacca cctcggctgc ggaagtgaaa tcggatttga cgctgacgcg
     1441 cgagccgggc gcgaatcttg tggtggtgcg cggcacgctc cccgcaaaag gcgcgccacg
     1501 caagctggtg ctggcgattg aagagccggc gctgcatgcc gcgtcgctgc ttgcagggtt
     1561 gctctccgaa cgcggtgtaa agctcgatgg aaagattcgc gccgttcatg ttgcagatac
     1621 cgaccgaacg ccacggacga ttctcgccga gcacgtttcc gtgccgctcg gcgattccgt
     1681 gaagctcgtc aacaagatca gccaaaacct gcacaccgaa atgctgctgc gaaccgcggt
     1741 gcggcaaaac ggcttgtgga gcactccgga agaaatgctg aaaactccgc aggactttta
     1801 tgccgcggca ggaatcgcgc cggacgacgt catgcaaacg gatggctcgg ggctctcccg
     1861 gcacgacctg gtcacgccgc gcgcaatcgc gactctgctc cgttacgcgc aggggcaaac
     1921 gtggttcgcc ccgtactatc gatcgctgcc ggtggcggga gtcgacggct cgctgcagga
     1981 ccgcatgaag gctacggtag cggccggaag aattcatgcg aagacgggat cggtcgagca
     2041 tgtgcggacg ctctcgggtt tcgcggaaac ccccagcggg cgccgcctga tcttttcgtt
     2101 tctcggcaac aaccagggcg gaggaaatca cgaagcggcc gatgtcttga acgggctttg
     2161 tgtggccatg attgaggagt ttgatgaggc cgccgctgcc atcgtgcctg cgcacgagcg
     2221 caaaaaacag agatgaaccc tcgcgggggc catcgggact ctttctaacg aaatgggaag
     2281 cagtcgaaaa acaaaaatgt cgcgaacggc acaagacgta ctggagtttg acaagctgct
     2341 cgaacttttg cggttgagga ccacttcggc gccggggcgg cgggctgtgg atgcgctgga
     2401 gtttttaacg aaccgcgtgg cgctagaagc ggcgtttagg ttgattcgcg aagcgcggga
     2461 atggctgcgc gggggcggcg agttgggatt cggcgggctg gccgatcccg agggatggct
     2521 cgaaaaaata gcggggccag gtattgttct ggaggcccgg gagctcttgg atgccgcttc
     2581 gctcctggaa accgcggcag ggttgcgcgg acagttccgg gaagaagcgg cgaaatttcc
     2641 cttgttggcc gcgcgcgtcg cttcgctggg ggattttcgg gaggcgctgg cggccatccg
     2701 tcggtgcgtg ttgccgaacg gcgagattgg cgaccaggct tcctcggcgc tgcggcgcat
     2761 tcgcgcgagc atcaccgaga cgcgggaagc gattcagaaa acgctcaaac aaatcctgcg
     2821 atcgcgccat gcggaggccg gggaagatta cgtcacgctg cgcaatgaaa gatttgtaat
     2881 tcccgtgcgc gcggaaagcc ggcgcagtgt gccgggcatc atgcatggag ctagcgctac
     2941 cgggcagacc gtgtttctcg agccgttcga gaccgtcgaa gtgaacaacc agctcgtgca
     3001 gcttgccgag gacgaagccg cggagatcct gagaattctg cgtgaactga cggaacgatt
     3061 gcaggtcgtt cgcgaaccct tgctcgtggc ggcggagacc attgcggagc ttgacggcgt
     3121 gttcgctcgc gggcgctttg cgcgcgattt cgatgcgtcg atgccggagt tttctgacgc
     3181 ttcggaattg cgtttggtcg aagcgcgaca tcccgtgctg gaagacaagc tgcgccggga
     3241 agagcgcgcc gttgtgcccc tgaccctggc gctgggcggc gcggaaaaag ttttggtcat
     3301 cagcggaccg aacaccggcg gcaagaccgt cgcgctgaaa acgaccgggc tcgcggcgtt
     3361 ggccgcgcag tctgctattc ccgtggcggc gcaacgtgcg gtgttgccgg tttttgatcg
     3421 cgtgctggtg gacatcggcg acgagcagtc catcgccgcc gacctttcga cgttttcggc
     3481 gcacatgctg aatttgaaga ccatgctcgg actggcgacg cccgcatcgc tggcgctcgt
     3541 tgacgaaatg ggcaccggca cggcgcccga agagggtgcg gcgctggcgg tggcgctgct
     3601 cgatgaattt cgcgccaaga attgcatcgt gctggccacc acccatcacg accgattgaa
     3661 aacttacgct tcgaccacgc cgggcgtggt caacgcagct gtcgaatttg acgacgtaaa
     3721 cttgcggccg acctaccgcc tgatggtcgg cgtgccg
//
LOCUS       DATMNS010000241         2763 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3042665, whole genome shotgun sequence.
ACCESSION   DATMNS010000241 DATMNS010000000
VERSION     DATMNS010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3042665"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1670)
                     /gene="ligD"
                     /locus_tag="VNW28_08485"
     CDS             complement(<1..1670)
                     /gene="ligD"
                     /locus_tag="VNW28_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HXA10005.1"
                     /translation="MPVGKTKSTHVRAGKPLKEYAGKRDFARTPEPAGSPNRGEGREF
                     VVQKHAARRLHYDLRLEMDGVLKSWAVTKGPSLTVGEKRLAVRTEDHPIAYLNFEGNI
                     PKGEYGAGSMIVWDRGRWEPEGDPQKGLAKGHLAFTLDGTRLKGRWHLVRMQPRSGEK
                     TEPWLLIKSDDEFGQHIGDREITDEEATSQISGRTNEELAAAGELRNDHAARVGVAKA
                     RKASLPNVGKTSGARAGLLPAFLEPSLASPCERPPSGPKWIHEIKYDGYRMQARIDGR
                     TIRLLTRKKLDWTERFRSIADALKAFGLGSAWIDGEIVVEDASGLSSFNDLQADLKTG
                     RPDRFRYFVFDVLYCEGLDLTKVVLKDRKDLLQRLFTILPPGSPIRFGEHLDVDGPTM
                     LEHACRFGLEGIISKRADLPYRPGRGDHWLKSKCVERQEFVILGYIPSTVTSRAVGSL
                     ALGYYDNQNLVYAGRVGTGWSQEQARALRDELEIISAIKPSFAKPLPAGTEKGVRWVE
                     PRLVCEIEYRGWTRDRLLRAAAFKGLRDDKSAEEIVLEAAPKGSKSRVP"
     gene            complement(2149..2358)
                     /locus_tag="VNW28_08490"
     CDS             complement(2149..2358)
                     /locus_tag="VNW28_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10006.1"
                     /translation="MPDAYVIEVSGRTAGIVARDSQNTSFNFYSALPRFNAIEGQRFS
                     DPLAAERAARMLAKHGSLPQRRETE"
     gene            complement(2446..>2763)
                     /locus_tag="VNW28_08495"
     CDS             complement(2446..>2763)
                     /locus_tag="VNW28_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10007.1"
                     /translation="PAYEVQCAWLAKTVDCVVNGNAQETPSQGWKTFLRNHAEGIASI
                     GLFVVRKDCGGIPGSYAMLEAVVDPNPPNHAEAKRFSPKFLLNDTVAPAERVIPALAI
                     GFC"
BASE COUNT          499 a          861 c          798 g          605 t
ORIGIN      
        1 ggaacgcgcg acttcgatcc cttcggcgcc gcctccagaa cgatttcctc agcagacttg
       61 tcatcccgga ggcccttgaa ggccgccgcc cgaagcagtc tgtcccgggt ccaaccgcgg
      121 tattcgatct cgcagacgag gcgcggctcg acccaacgga cgcctttttc ggttccagcg
      181 gggagcggtt ttgcgaaaga tggcttgatt gcgctgatta tctcaagctc atcgcgcagc
      241 gctctcgcct gctcttggga ccagcctgtc ccgacccggc cggcgtaaac aaggttttga
      301 ttgtcataat acccgagcgc cagtgatccg acggcccggc tagttaccgt cgaggggata
      361 tagcccagga ttacgaactc ctgtctctcg acgcatttac tcttgagcca atgatcgccg
      421 cggccgggtc gatagggcag gtccgcgcgt ttggaaataa tcccttcgag tccgaatcgg
      481 catgcgtgct ccaacatggt tggcccgtcc acatcgagat gctcgccaaa gcgaatgggc
      541 gagccgggcg gcaaaatggt gaagagccgc tgcagcaggt ctttgcggtc cttgagaacc
      601 actttcgtta gatctaaccc ttcgcagtag agaacatcga atacgaagta tcggaaccgg
      661 tccgggcgac cggttttcag atcggcctgc aaatcattga agctcgatag gccgctcgca
      721 tcctcgacga cgatctcccc gtcgatccac gccgagccaa gaccgaatgc cttcaatgcg
      781 tccgctatgc tgcgaaagcg ttcagtccag tcgagctttt tgcgcgtcaa tagacggatt
      841 gtgcgcccgt cgatccgcgc ctgcatgcgg tagccgtcat atttgatttc atggatccat
      901 ttcgggccgc tcggaggtct ttcacacggc gatgcgaggc tcggctcgag gaaagccggg
      961 agcagtcccg cgcgagcgcc ggaagttttg ccgacgttcg gtagggaagc ttttcgcgct
     1021 ttcgccaccc cgactctggc cgcgtgatcg ttccgtaact cacctgcggc cgcgagctct
     1081 tcgttcgttc ggccgctgat ttgtgatgtt gcctcctcgt cagtgatttc ccgatcgcct
     1141 atatgctggc cgaattcatc gtccgacttg atgagcagcc atggctcggt cttctctccg
     1201 gatcgcggct gcatccgaac caggtgccaa cgccctttga gccgcgtgcc gtcgagcgta
     1261 aacgccagat gccctttcgc aagccccttt tgcggatcgc cctccggctc ccatcggccg
     1321 cggtcccaga cgatcatcga gcccgcgcca tattcgccct tcggaatgtt gccctcgaaa
     1381 ttgaggtatg cgatcgggtg gtcttcggtg cggaccgcaa gccgcttttc gccgaccgtg
     1441 aggctcgggc ctttggtgac cgcccagctt ttcaagactc cgtccatctc gagccgcaga
     1501 tcgtaatgga gccgtcgcgc cgcgtgtttc tgcacgacga attctcggcc ctcgcccctg
     1561 ttaggcgaac ctgcaggctc cggagtccgg gcgaagtcgc gcttcccggc atattctttg
     1621 agtggcttcc ccgctcggac gtgtgtgctt tttgtcttgc cgaccggcac ggctggtctc
     1681 cgtgcattgc ctcggcgagg cggacggcca tttggtcgcg ggacaggtgg tttcctcgcc
     1741 cggcgagtat gaatgtgcgc ttcatggcgc cctccctcgg tttgcctcca cccgttctcg
     1801 cgcgggctcc catcggtttc ttaagactgc cccgccaaaa gcgcaaactt ctaagcggcg
     1861 cgcggagctt tcagggcgcc gccgtcaatc ttcactcctg tccctgtcgg aaatcaaatc
     1921 cgatcatgct gatggtgtaa tccgccgaga atccgtgtcg ggagaatgca cctggcagcc
     1981 tgaacatccc gtcgtaccgg cgcatccttg agatatgtcc gggcggctct gtcacattac
     2041 caatcagagc gcgtaacgaa gagacaccgg aacgcatcgc cctccgcgaa atcggcgaga
     2101 cattagcgct ccccagatgc cgatccttcg ttcctacttg tccgctgatt attccgtttc
     2161 gcggcgctgc ggcaggctcc cgtgtttcgc aagcatgcgc gccgcacgct cggctgccag
     2221 agggtcggaa aaacgctggc cttctatggc gttaaagcgg ggtagcgcgg aatagaaatt
     2281 gaaactggta ttttgggagt cgcgggcgac aattcccgca gtacggccgg aaacttcgat
     2341 gacataggcg tcgggcatgt aagtttcctt ctgctggcct cagtgaacac acaaatttca
     2401 ggccgcacaa gccgcttgct ctgccagtcc gccgggcacc tccggctaac agaacccaat
     2461 ggctaacgcg ggaatgacac gctctgcagg cgcaaccgtg tcattcaaaa ggaatttggg
     2521 ggaaaatcgt ttggcttcgg catggttcgg ggggttggga tcgactacgg cttcgagcat
     2581 cgcataggag ccgggaatgc cgccgcaatc cttccgaacg acaaacaggc ctatcgatgc
     2641 gatcccctcg gcgtggttgc ggaggaacgt cttccaccct tgagatgggg tctcctgtgc
     2701 gttgccatta acaacgcagt caactgtttt ggcgagccag gcgcactgga cctcgtacgc
     2761 cgg
//
LOCUS       DATMNS010000242         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_708586, whole genome shotgun sequence.
ACCESSION   DATMNS010000242 DATMNS010000000
VERSION     DATMNS010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708586"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VNW28_08500"
     CDS             <1..532
                     /locus_tag="VNW28_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10008.1"
                     /translation="IYGSGARKFSVIFVRTLPRQFVGDETTSALLIGLILASRFRFTF
                     IEAGQELMEKLGDDVTDAEFQLACRQIIYDIERMEHESSEFGMNSPDLFQLAFGPENH
                     EIVNGFYEIWFPVRKDLFALLERRLGDPSTASRENVREEIKKFTNTVSPYNRRFLEMC
                     LQRYTLYLKGWLKKPE"
     gene            complement(651..1775)
                     /locus_tag="VNW28_08505"
     CDS             complement(651..1775)
                     /locus_tag="VNW28_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA10009.1"
                     /translation="MVAERADAPAVARAPASEPVVRIEGLNHYYGEGEARNQVLFDNR
                     IEIPAGQLVVMTGPSGAGKTTLLTLIGALRSIQEGRIEVLGRDLSRLGGGELVGVRRD
                     IGFIFQMHNLFDALSAFENVKMAAQLGDTPPAEMRQRGAGILERLGLGHRIDYKPRFL
                     SGGERQRVAIGRALVNHPRLVLADEPTAALDKDSTVNVINLLKQTTVEHGSAVMMVTH
                     DHRIIDCADRLVHMVDGRIMSDVVLHDALRICEFLRPIDLFKTLTPRQLTDVAERMTK
                     RHYSTGEIIIREGEPGEEFFLISEGQIDVIRAEHEVARLRPGDFFGEVALISGEPRNA
                     AVVAVTEVDTYVLGKTDFQTAIATSQSFRDQLYRVYFMRH"
     gene            complement(1787..>2797)
                     /locus_tag="VNW28_08510"
     CDS             complement(1787..>2797)
                     /locus_tag="VNW28_08510"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXA10010.1"
                     /translation="SLEVIRGLDGDIFIQSAHKYQFATRDPFPSSELDAARSAPGVAS
                     ARPLYADWFDFFWKNPFDGKIFLVRAFGFDPGAPVFLFPEVDANREKLKEGSTIIVDR
                     RARGFLGMDRGPTEAELNGANVKIVGSFALGPDFQSDGTVMMSDRTFASLLRGPAGNQ
                     SEVDLGVIKVRSGDDPATVRQALSKTLPSTIAVFTKDELMEFERKFQAAVSSAGPIFA
                     MGTIVGFVVGMLISYQVTYTDLSDQLPQYATLKAMGYRTGYLLRVVLEQAAFNALAGW
                     IPAWLLSILLYYVVGRVALLPLHMSVKVTLLSLGLTLGMCLISAVLAVRRVIKADPAE
                     VF"
BASE COUNT          609 a          817 c          814 g          557 t
ORIGIN      
        1 tatttatggt agcggagcgc gaaaattttc agtcatattc gtacgaacct taccaagaca
       61 gttcgtaggc gatgaaacga cctcggcgtt attgatcggg ctaattttgg cttcgagatt
      121 tcgcttcacc tttatcgaag cgggtcagga gctgatggaa aagctcggcg acgatgtcac
      181 agacgccgag tttcagctgg cctgtcgcca aataatctac gatatcgagc gtatggagca
      241 tgaatcgagt gaattcggaa tgaatagtcc ggatctcttt caactggcct tcggccctga
      301 aaatcacgaa atagtgaatg ggttttatga gatttggttc ccggtaagaa aggatctctt
      361 tgccttgtta gaacggcgcc tgggagatcc gagtaccgcg tcccgcgaga atgttcgaga
      421 agagatcaag aaatttacta atacagtgag cccttacaat cggcgttttc tggaaatgtg
      481 cctacagaga tatacacttt atctcaaggg ttggttgaaa aaacctgagt gatctacgat
      541 tgagccgaaa gccgtcgtct cgatggacgc gccgcggtcg tccagcgaat attcaattgc
      601 ccccacacgc gccttcgccg ggacggcagg aatggcgcaa ttcttcaagg tcaatgccgc
      661 atgaaataga cgcggtaaag ctggtcgcga aagctctggc tggtggcgat cgcggtctgg
      721 aaatcggttt tgccgagcac ataggtgtcg acctcggtga cggcgacgac ggcagcgttg
      781 cgcggttcgc cgctgatcag cgcgacttcg ccgaagaaat cacccggcct caggcgcgcg
      841 acctcatgct cggcgcggat cacgtcgatc tggccttcgg aaatcagaaa gaactcctcg
      901 ccgggttcgc cctcgcgaat gatgatttca ccggtggagt aatgtcgctt cgtcatcctt
      961 tccgcaacgt cggtgagttg tcgcggcgtc agggtcttga acaaatcgat cgggcggagg
     1021 aactcgcaaa tccttagcgc atcgtgcagg acgacgtcgg acatgatcct cccgtcgacc
     1081 atgtggacga ggcgatcggc gcaatcgata atccggtgat cgtgggtgac catcatcacc
     1141 gccgagccat gctcgactgt cgtctgcttg agcagattga tgacgttgac tgtcgaatcc
     1201 ttgtcgagcg ccgcggtcgg ctcgtcggca agcaccagcc tcgggtggtt caccagggcc
     1261 cggccgattg cgacccgctg gcgttcgccg cctgacagaa atctcggctt gtaatcgatg
     1321 cggtggccga gcccgaggcg ctccaggatc ccggcgccgc gctgccgcat ctcggctggc
     1381 ggagtgtcgc cgagttgcgc cgccattttg acgttctcga aggcggacag cgcgtcgaac
     1441 aaattgtgca tctgaaaaat gaaaccgatg tctcgccgca ccccgacgag ttcgccaccg
     1501 cccaaccgcg acaaatcccg gccgagcacc tcaatcctcc cctcctggat cgaacgcaac
     1561 gctccgatca aggtcaagag ggtagtcttg ccggcccccg agggaccggt catcacgacc
     1621 agctgacccg ccgggatctc gatccggttg tcgaacaaga cctggtttcg cgcctcgcct
     1681 tcaccgtaat agtggttcaa cccctcgatc cggacgacgg gctcggatgc gggtgcccgc
     1741 gcgaccgcag gcgcatcggc ccgttcggcg accatcgcag cgcacatcag aacacctcgg
     1801 cgggatcggc cttgatcacc cgtcgcacgg cgagcacagc tgagatgagg cacatgccca
     1861 gggtcaagcc gaggctcagt aaagtaactt tcacgctcat gtgcagaggc aacagcgcga
     1921 cgcggccgac gacatagtag agcagaatgc tcagcagcca tgccgggatc cacccggcaa
     1981 gggcgttgaa cgccgcctgt tcgagtacga cgcgcagcag ataaccggtt cgatatccca
     2041 tggctttcaa tgtcgcatat tgaggcagct ggtcggaaag atcggtatac gtgacctgat
     2101 atgaaatcag catcccgacg acaaagccga cgatggtccc catggcaaag atcggtccgg
     2161 ccgaggacac ggccgcctga aatttgcgct cgaattccat cagctcatcc ttggtgaaga
     2221 ctgcgatcgt gctgggcagc gtcttgctca gcgcctgtcg cacggtcgcc ggatcatcgc
     2281 cggaacgcac ctttatcact ccgagatcaa cctcggactg gttaccggcg gggccccgga
     2341 gcagactggc gaaggtgcgg tcgctcatca tgaccgtacc atcgctctga aaatcggggc
     2401 cgagggcaaa gctgccgacg attttgacgt tggcaccgtt cagctcggcc tccgtcggac
     2461 cgcggtccat cccgagaaaa ccccgggccc ggcgatcgac gataatggta ctcccttcct
     2521 tcaacttctc ccggttcgcg tctacctctg gaaacaggaa gactggcgca cccggatcga
     2581 agccgaaagc gcggacgaga aaaatcttac cgtcgaatgg gtttttccaa aaaaagtcga
     2641 accagtcggc gtagagcggc cgtgcgctcg cgacgcccgg tgcgctccgc gcagcgtcga
     2701 gttccgaact gggaaacggg tccctggtgg caaattggta cttatgcgcg ctctggatga
     2761 agatatctcc gtcaagcccg cgaataacct caaggga
//
LOCUS       DATMNS010000243         7988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1986515, whole genome shotgun sequence.
ACCESSION   DATMNS010000243 DATMNS010000000
VERSION     DATMNS010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7988
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1986515"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VNW28_08515"
     CDS             complement(<1..558)
                     /locus_tag="VNW28_08515"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HXA10011.1"
                     /translation="MAAEQSSNAELLEIVAAAEPIARQLQALRQGTRPVAFSHVVAAA
                     RSFLVALIAGKIPKTLWVICPNARTQELLHESLTNWRPDALFLPEAEFLGVENILPDQ
                     EIAAERLALLSRVEESNRPQVIVATRASLDQPAPRRGLLRATALRLRRAASQPLERLV
                     DSLGEAGYERVAQVTTRGQFAVRGGI"
     gene            complement(581..1597)
                     /gene="aroF"
                     /locus_tag="VNW28_08520"
     CDS             complement(581..1597)
                     /gene="aroF"
                     /locus_tag="VNW28_08520"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXA10012.1"
                     /translation="MIIITQPTVTDAQIDHIVARIEEFGLRPQVIRGETRVVIGVIGP
                     EDLIRGKPIAAIPGVESVVPVMKPYKLVSQENGSLPPPVLIGGVKIGSGQRVVLISGP
                     CSVESKQQLLSIANSVKEAGARVLRGGAFKPRTSPYSFQGLGEDGLRFLAEARAETGL
                     PIITEIMDTRDLPVIEKYADCLQIGARNMQNFALLKEVGRSRLPVLLKRGLSATINEL
                     LMSAEYILSEGNPNVLLCERGIRTFETMTRNTLDLNAVPILKAKTRLPVVVDPTHGIG
                     LRDYVPQMALAAVAAGADGLMIEVHNEPDRARSDGEQALRPEIFSDLVRRIRAVAEAV
                     GREL"
     gene            complement(1670..2293)
                     /locus_tag="VNW28_08525"
     CDS             complement(1670..2293)
                     /locus_tag="VNW28_08525"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXA10013.1"
                     /translation="MSLDYSRLRPFVALGALAFLALAGCAEEDPRLAYGQTQYLGGVY
                     GNEPVASRAPNDNVSYWDGDGVPGKPSIKISLGEQRAYFYKSGQLVGVSQLSTGREGK
                     NTPVGKYRITEKDIDHASSIYGDYVDAGDNVVVPNIDKNVDPKPPGTHYKGAPMPYYM
                     QIAPGFGMHAGYLPGYPASHGCIRMPEFMAEDFFKSVEKGTPVSITP"
     gene            2438..2797
                     /locus_tag="VNW28_08530"
     CDS             2438..2797
                     /locus_tag="VNW28_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HXA10014.1"
                     /translation="MPTYEYACQKCGKNFDAYQSMRDEAYKICPKELCRQSKWGKGKV
                     TRLLGTGAGLIFKGSGFYITDYRSNSYKEAAKKDSSSGGEKSSGESAAGKIEGKPKAP
                     AAAAQNSDAKTRKDSDS"
     gene            2989..3534
                     /locus_tag="VNW28_08535"
     CDS             2989..3534
                     /locus_tag="VNW28_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10015.1"
                     /translation="MLGKVLLGACVAAALIEMILPPDLPPPPKSYGFAPMIGMDMVSA
                     ISSHQAASLVYSEEQINSYIGSNLHRKDSPAKEGVFPLVRIFVQLDEGICRINIERQF
                     YGLSIYSGGSYRVAIGNGKVSGEATGGYIGHMPIHPKLMKYADLLLQKAWDALARERN
                     SVARFAGIEFHPQSVTLIVTR"
     gene            3622..3765
                     /locus_tag="VNW28_08540"
     CDS             3622..3765
                     /locus_tag="VNW28_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10016.1"
                     /translation="MLPSTLTPPTAPLISNPGSNRQSERDQPSRRDCQHESFLMFVVF
                     FLY"
     gene            3845..5146
                     /locus_tag="VNW28_08545"
     CDS             3845..5146
                     /locus_tag="VNW28_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10017.1"
                     /translation="MPAPSRTLLFLAAVSCLTAGAAFPAGKPSSTSASRQFLVYGGEV
                     SLRGAMCDLAERTKANLLRLLALRDNWKTPLIINLDYPWANFPDAPTVQLDISQLGFG
                     LKLQLNLLVDDKFKGRAVQRELLRAILIEMMYRDRGNIAAGTPYVAPPDWLVDGMLKL
                     QPGHDSDADARLLQIIVASNRIMPLEDIVRQRRPQLGAASRKLDDACAMALLQLLLDS
                     PGGRQKLAQFIVDLPAAPNDALADLRVHFPETLGQSPRRWWTLSVAHLSATDRYEMLS
                     AAQTAARLDRLLRFSITAPDGTSRDYSLGDYETFRKLPGHRAVLRQVSQQLQVLSARA
                     HPSYRLLVQEDYELAELLARGKTRKVRARLERVASYREVIEHQAREIDDYLNWYEATQ
                     TKAMSGAFSQLLEAARAGDEAQPRRRDPISIYLDSIEMEMN"
     gene            complement(5389..5652)
                     /locus_tag="VNW28_08550"
     CDS             complement(5389..5652)
                     /locus_tag="VNW28_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10018.1"
                     /translation="MGRTADSADQFARAIELAGENAAYHFNLANVEFMLRHDLSAAWR
                     VDEPDLLRRALAEFREASRLSPRDMEYARAYAESFYGTPDADW"
     gene            6009..6446
                     /locus_tag="VNW28_08555"
     CDS             6009..6446
                     /locus_tag="VNW28_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HXA10019.1"
                     /translation="MQKIGFAEALDAIVATDRRYHRDAYVFLRDALDYTTKQQKKSKG
                     AAVRHVAGPELLEGVRLYALKEFGPMALTVFSYWGIERCEDIGRMVFNLIGAGIFGKT
                     EQDTLEDFKSVYSFHDAFVKPFLPETAMTPTKFPPALPAPKLS"
     gene            6484..>7988
                     /locus_tag="VNW28_08560"
     CDS             6484..>7988
                     /locus_tag="VNW28_08560"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HXA10020.1"
                     /translation="MLISARLNPAVPGSSPAITFPPVTAQQWTLPNGLTIIVQTDRGA
                     PVASVQAWCGTGSINEGRHLGAGLSHILEHMLFKGTKTRTTNAIAQSVQDEGGYINAY
                     TSFDRTVYWIDVPKEGVTRALDILSDAMMNSTLPPAEYSKEQEVIRREFAMSYDDPDR
                     MVGQLMFKTAYQRHPYRLPVIGEIQIFDQLTQQDVLRYYQTRYVPNNLTFVVVGDVDA
                     GAIHAQLRQYFAAYPARSLEPVFIPEEPPQQGRREVNEEFATELTRLALAWHIPEITH
                     PDVPALDLLSTILGDGRSSRLYREVREEAGLVFGISAFSYTPGDPGVLGVDATLDPTK
                     RAEAERLILQIIAKLGAEGVTAEELAKAKKISLGHSLGLLTTMRGQASDLGSNWLVTR
                     NLNFTRDYLGAMQKVSREEIQRAAAQYLVEDNLTVVSLNPRGTLAAKTPETTAGSAGE
                     VEKFELSNGLRLLVREDSRLPLVSMVAVFRGGLLAETRATSGLTRLMAKVLV"
BASE COUNT         1571 a         2460 c         2418 g         1539 t
ORIGIN      
        1 aatcccgccg cggacggcga actgtccgcg cgtcgtaacc tgcgcgaccc gttcgtagcc
       61 ggcttcgcct aacgaatcga cgaggcgctc gaggggttgc gacgcggcgc ggcgcaggcg
      121 caaagcagtc gcccggagca ggccgcgccg gggcgccggc tggtcgagac tcgcgcgcgt
      181 tgccacgatg acctggggcc ggttactttc ttcgacccgg ctgagaagcg cgagtcgttc
      241 ggccgcaatc tcctggtccg gtagaatatt ttcgacgccg agaaactcag cctcgggcag
      301 gaagagcgcg tcgggtcgcc agttcgtgag gctttcatgc aacaactcct gggtgcgggc
      361 gtttgggcag ataacccaga gggtcttggg gatcttaccg gcaatgagtg cgaccagaaa
      421 agaacgtgcc gctgcgacga catgcgaaaa cgcgaccggc cgcgtaccct gcctgagtgc
      481 ctgcaactga cgcgcgatcg gctcggcagc ggcgacgatc tcgagaagtt ccgcgttgct
      541 cgattgttcc gccgccatcc tgtcgccgtg cggaaatgcg ttagagctcg cggccgacgg
      601 cttcggcgac cgcccgaatt cgcctcacga ggtcgctaaa gatttcgggg cgcagtgcct
      661 gttcgccgtc gctccgcgcg cggtcgggct cgttgtgcac ctcgatcatc aacccgtcgg
      721 ctcccgcggc gaccgctgcc agcgccatct gcggaacgta atcgcgcaaa ccaatgccgt
      781 gcgtcggatc gactaccacc ggcaggcgcg tcttcgcttt cagaatcgga acggcgttca
      841 aatcgagggt gttgcgcgtc atggtttcaa aggtgcggat gccgcgctca caaagcagga
      901 cgttggggtt tccttcgctc aggatatatt ccgcgctcat caggagttca ttgatcgtcg
      961 cgctcaagcc gcgcttcagc agcaccggca gccggctgcg cccgacttcc ttcaagagag
     1021 caaaattctg catgttgcgc gcaccgatct gcaaacaatc cgcgtacttt tcgatcaccg
     1081 gcagatcgcg cgtgtccatg atttcggtga tgatcggcaa gccggtctcg gcccgcgctt
     1141 cagcgagaaa gcggagtcca tcttcgccca ggccttggaa actgtaaggc gaggtccgcg
     1201 gcttgaaagc tccaccacga aggacgcgcg cgccggcctc ttttactgag ttcgcgatcg
     1261 agagcaactg ttgtttgctc tcgactgagc agggtccgga gatcagcacg acgcgctggc
     1321 cgctgccaat tttcactcca ccgatcaaca ccggcggcgg caaggacccg ttctcctggg
     1381 aaacgagttt gtagggcttc atcacgggga cgaccgactc gacccccggg atggcggcga
     1441 tcggtttgcc ccggatcagg tcttcgggac cgatcacgcc gatcacgacc cgcgtctcgc
     1501 cccggatcac ttgcgggcgc agaccgaatt cctcgatccg cgcgacgatg tggtcgattt
     1561 gcgcatcggt cactgtgggt tgagtgatga tgatcatggc gcgaagagca ggagtgtaac
     1621 ctctgcgcga cggggggcaa cccttgtgct acgtggcgaa aaccgaccgc tagggagtaa
     1681 tgctgacggg ggttcctttt tcgacagact tgaagaaatc ctccgccatg aattccggca
     1741 tccggataca gccgtgcgat gccggatagc ccgggaggta accggcgtgc atgccgaagc
     1801 cgggagcaat ttgcatgtaa tagggcatcg gtgcgccctt gtaatgggtg ccgggcggtt
     1861 tcggatcgac attcttgtca atgttaggca cgacgacgtt atcacccgca tcgacgtaat
     1921 caccgtaaat gcttgaggca tgatcgatat ctttctccgt gatcctgtac ttgccgactg
     1981 gcgtattttt gccctcccgt ccggtggaca attgcgatac tccgaccagc tgcccacttt
     2041 tgtaaaaata ggcgcgctgc tctccgagac tgatcttgat cgacggtttc ccgggaacgc
     2101 catccccatc ccaatacgag acattgtcgt tcggcgcgcg cgaggcgacc ggctcatttc
     2161 cgtacacgcc gccgagatat tgcgtttgcc cgtaagccag ccggggatct tcctccgcgc
     2221 agcccgcaag ggcgaggaaa gcgagggcgc cgagcgccac gaacgggcgc aatcgagagt
     2281 aatcgagaga cattttcact agcgggaagc ggaactaacg cgggagcttt tcgaaagtca
     2341 agacagcagg gcgcgggccg aacgcggggg gacggcttga attctgctta attttgctga
     2401 gactggcgac cgtcgcccaa ttatctggtt cgaacccatg cccacctacg aatacgcctg
     2461 tcaaaaatgc ggaaagaatt ttgatgccta tcaatcgatg cgcgatgaag cctataagat
     2521 ctgcccgaaa gagttgtgtc gccaaagcaa atggggcaaa gggaaagtca cgcggttact
     2581 ggggaccggt gccgggctga ttttcaaggg ctctggtttc tacatcaccg actaccggag
     2641 caactcttac aaagaggcag ccaagaaaga ctcatcgtcg ggtggcgaga aaagcagtgg
     2701 ggaaagtgcc gcgggcaaga tagagggcaa acccaaagcc ccggctgcgg ccgcgcagaa
     2761 ttcggacgcg aaaaccagga aggattccga ctcgtgacca agctcgcttg ttcagattgc
     2821 cagtacgaaa acgagtcaga gcgaatctac tgccacaact gcggtgcgcg cctcgaccgg
     2881 agcgcaatcg cccgagcgaa cgtcgccgag ggtgttgatg agagcacgca aaagcatctg
     2941 aagaagatgt tcgattcggg aagcgggcgc gcaggtcggg cggccgttat gttgggaaag
     3001 gtactgctcg gcgcctgcgt cgccgcggcc ctcatcgaga tgattctacc gccggacctg
     3061 ccgccgccgc ccaagagtta cggtttcgcg cctatgattg gcatggacat ggtctcagcc
     3121 atctccagtc atcaagctgc ctcactggtt tacagcgaag agcagatcaa cagctatatc
     3181 ggttccaacc tgcaccgcaa ggacagcccc gccaaggagg gcgtcttccc gctcgtccgc
     3241 atctttgtgc aactcgacga gggaatctgc aggatcaaca tcgaacgcca attctacgga
     3301 ctctccattt acagcggggg ctcgtatcgt gtcgccatcg gcaacggcaa agtcagcggc
     3361 gaggccaccg gcggctacat cggccacatg ccgatccatc cgaagctcat gaagtatgcc
     3421 gacctcctcc tgcaaaaggc gtgggacgcc ctggcgcgcg agcgcaactc cgtcgcaagg
     3481 ttcgccggca tcgagtttca tccgcagtcg gtcacgctga tcgtgacgcg gtaaagaaac
     3541 cctgcggccg gcgagagaac ccaggttagc cgaccccttg gccgaggccc atcgtggcca
     3601 gcagttgacg cgaatgggct tatgctgcca tctactctca ctccgcccac cgcgccgctc
     3661 atctccaacc ctggctccaa tcggcagagc gaacgcgatc aaccctcccg ccgcgactgc
     3721 cagcacgaaa gctttctcat gttcgtcgtt ttcttccttt attaaactcg gataaaacag
     3781 cacaaaggag gctaatttcc gcgctgcgat tgcaaaaaaa tcatatcctt cgccttcctc
     3841 cccaatgccc gcgccgagcc gaactctcct tttcctcgca gctgtcagtt gcctgaccgc
     3901 cggtgccgcg tttcccgcgg gcaagccgag cagtaccagc gcctcgcgcc agttcctggt
     3961 ctacggcggc gaggtctccc tccgcggggc gatgtgcgac ctcgcggaac gaaccaaggc
     4021 aaacctgctg cgtttgcttg ctctccgcga caactggaag acacctctca tcatcaacct
     4081 cgattatccg tgggccaact tcccggatgc gcccacggtc caactcgaca tcagccagtt
     4141 gggatttggc ctcaaactcc agctcaatct gctcgtcgac gacaaattca agggccgtgc
     4201 cgtgcagcga gaattgctcc gggcgatttt gatcgaaatg atgtatcgcg accggggcaa
     4261 catcgctgcc gggacgccct atgtcgcgcc gcccgactgg ctggtcgatg gcatgctgaa
     4321 gctgcaaccc ggtcacgatt ccgatgccga cgcgcgactg ctccagatta tcgtcgcttc
     4381 gaacagaatc atgcctctcg aagatatcgt caggcagcgg cgcccgcagc tcggtgcggc
     4441 gtcgcgcaag ctggacgacg cctgcgccat ggcgctcctc caactcctgc tcgattcccc
     4501 cggcggccgg caaaagctgg cgcagtttat cgtcgatctg cctgccgccc cgaacgacgc
     4561 actggccgat ttgcgcgtgc atttcccgga gacgctcggc caatccccgc gcagatggtg
     4621 gaccttgagc gtggcccatc tctccgcgac cgatcgttat gagatgttaa gtgccgcgca
     4681 gacggccgcc cggctcgatc gcctgttgcg cttctccatc acggctcccg acggcacatc
     4741 gcgcgattac tcgttaggcg actacgagac cttccgtaaa ctgccgggcc accgggccgt
     4801 tctccggcag gtcagtcagc aactgcaagt gctgagcgcg cgcgcgcatc cctcataccg
     4861 gttgcttgtg caggaggatt atgagctggc ggaactcctc gcgcgcggca agacgcgcaa
     4921 ggtgcgcgcg cggctcgagc gcgttgcgag ctatcgggag gtgatcgagc accaggcgag
     4981 agagatcgac gattatctca attggtatga agccacccag accaaggcaa tgagtggggc
     5041 cttttcccaa ttgctcgagg cggccagggc gggggacgaa gcgcagccgc ggcgccgcga
     5101 tcctatttcg atctatctcg attcgatcga gatggagatg aattgaggct cacagcaggc
     5161 gggtcgcgcg gtgacacgcg ggagaaagcc ggcttgagtg tccaactgat ccgcctgtgc
     5221 caggagcttc cgtttcaaac cgtcgtgccg cgggtcgacg attcgattga ggcaacggcg
     5281 cgcttcggtg gcgtcggtgc ggtgcaagct gatccgggcg agttgcaagt aggcaaaatc
     5341 tccctggggc gaaagagcga gcacgtgctt ccacgcggcc gcggcctctc accaatccgc
     5401 gtcgggcgtg ccgtagaagc tttcggcgta agcgcgggca tattccatgt cacgaggcga
     5461 aagccggctc gcttcgcgaa actccgcgag cgcccggcgg agcaagtccg gctcgtctac
     5521 ccgccaggcc gcgctcaggt cgtgtcggag catgaattcc acattcgcga gattaaaatg
     5581 ataagcggca ttttcgccgg cgagctcgat cgcgcgggca aactggtccg cggagtccgc
     5641 cgtgcgtccc atgcgcagat aggctccacc taacgaaatt ggcgatcgtg gcgttctccg
     5701 gcgcgagccg ttgcgcggag ccccactgca tgatcgccgc gcgcagattt ccgttttcgg
     5761 agagaaactc cgcgtaggcc aggtgccctg ggaccgatgt cggatgctcc tgaagaaatt
     5821 tctcgtgtgc catttgggcg agttgcgggg accagccgaa aaaatggttc tcggccgggg
     5881 ccgtacgggc gagcgcgaag agcagagcga ggaggagcac cgaccgcata ttacgctctc
     5941 caccatttgc tcccgcggcg gatgctgatt atcatagcga ctcgctcgtc ctgagcagtt
     6001 cctttgccat gcagaaaatc ggatttgctg aagctctcga cgccatcgtc gcgaccgacc
     6061 gtcgttatca tcgcgacgcc tacgtcttcc tgcgcgatgc gctcgattac acgaccaaac
     6121 agcagaaaaa atccaagggc gccgcggtcc gccacgtcgc gggtccggag ttgctcgaag
     6181 gggtgcggct ttacgcgctg aaggaattcg gcccgatggc gctgaccgtt ttttcctact
     6241 ggggaatcga gcgctgcgaa gatatcggca gaatggtttt caacctgatc ggcgccggca
     6301 tttttggaaa aacggaacag gacacgctcg aggatttcaa atccgtctac agttttcacg
     6361 acgcgtttgt gaagccgttt ctgcccgaga ccgcgatgac gccgacaaaa tttccgccgg
     6421 ccctgcccgc gccgaagctt tcatgagcgg gacggttcgc gccggtggcc cgcctttcct
     6481 gccatgttga tcagcgctcg actcaacccg gcggtgccgg gcagctctcc ggccatcacc
     6541 tttccgccag ttaccgcgca gcagtggacg ctgcctaacg gcctaacgat catcgtgcaa
     6601 accgaccggg gcgcgccggt cgcgagcgtg caggcctggt gcggtaccgg cagcatcaac
     6661 gaagggcggc atctcggtgc cggtctttct catatcctcg agcacatgct cttcaagggg
     6721 actaagaccc gcacgaccaa cgcgatcgcg caaagcgtcc aggacgaggg cggctatatc
     6781 aatgcctaca cttcctttga tcgcacggtt tactggatcg atgtgccgaa ggaaggggtc
     6841 accagggcgc tcgacatttt gtccgatgcg atgatgaact cgaccctccc gccggccgag
     6901 tacagcaagg agcaggaggt gattcggcgt gaatttgcga tgagctacga cgatcccgat
     6961 cggatggttg ggcagctcat gtttaaaacg gcctaccaac ggcatccata tcgcctgccg
     7021 gtcatcggcg agattcaaat tttcgaccaa ctcacccagc aggatgtgct gcgctactat
     7081 cagacccgct acgtgcccaa caatctgacg tttgtcgtgg tcggagatgt ggacgcgggt
     7141 gcgatccacg cgcagttgcg gcaatacttt gccgcctacc ccgctcgctc gctcgagccg
     7201 gttttcatcc cggaggagcc gccgcaacag ggccggcgcg aggtgaatga agagtttgcc
     7261 acggaattga cgcggctcgc gctcgcctgg cacatcccgg agattaccca tcccgacgtg
     7321 cccgctctcg acctgctctc gacgattctc ggcgacggcc gcagctcgcg actctaccgc
     7381 gaggtgcgcg aagaagcggg cctcgttttt ggcatttccg cgttttccta cacgccgggt
     7441 gaccccggcg tgctcggggt cgatgcgacg cttgatccaa ccaagcgggc ggaggcggag
     7501 cggctgattt tgcaaatcat cgccaaattg ggagccgagg gtgtaactgc cgaggagctg
     7561 gcgaaggcga aaaagatttc gctgggccat tcgttaggct tactcaccac catgcgcggc
     7621 caggcctccg acctgggctc caattggctg gtcacccgga atctcaattt tacgcgcgat
     7681 tacctgggag cgatgcagaa agtctcgcgg gaggaaatcc agcgtgccgc cgcgcaatat
     7741 ctggtggagg acaatctgac ggttgtctcc ttaaatcccc gtggcacgct ggccgcaaag
     7801 acgcccgaga caaccgccgg ttccgccggc gaggtggaga aattcgaact ctcgaatggc
     7861 ttgcgtcttc ttgtgcgcga ggattcgcgg ctgccgcttg tttcgatggt cgccgttttc
     7921 cgcggcggtt tgctcgccga gacgcgggcg accagcggcc tgacccgctt gatggccaag
     7981 gtgttggt
//
LOCUS       DATMNS010000244        13486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4488732, whole genome shotgun sequence.
ACCESSION   DATMNS010000244 DATMNS010000000
VERSION     DATMNS010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 13486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13486
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4488732"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VNW28_08565"
     CDS             <1..408
                     /locus_tag="VNW28_08565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10021.1"
                     /translation="TPPLGNIAAPRALPVEGTPPGEARVAATPPTTLEAAKPSATEPE
                     VRRAELVHPEDLIASQTSANRVQITPVRKTYLRVTVDRDPQPAFDGWLDPSDPPLSFR
                     GRHVRVKVLERGAVQITKNGAVLAPGDADVTFE"
     gene            392..1780
                     /gene="rimO"
                     /locus_tag="VNW28_08570"
     CDS             392..1780
                     /gene="rimO"
                     /locus_tag="VNW28_08570"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HXA10022.1"
                     /translation="MSPLNSAPEADPSLTTVGMISLGCAKNLVDAEIMLGSVRERGMR
                     ITPHAAEADVLVVNTCAFIDSAKEESIEAILEAHQQRGLTSRPEQKLIVSGCMSQRFA
                     QELREQLPEVDAFIGLDQVRELGAIIERVLSAQSEPGENFVTARPTYIPDWDTPRFRL
                     TPAHSVYLKIAEGCNHPCSFCVIPQMRGRHRSRPPASVLAEIRRLVAEGVKEINLISQ
                     DTTYYGMDLWSAKAGPRQPVDSSRGPTLAALLRAIEEIEGDFWVRLLYTHPAHWSDEL
                     IETIAQCGKVARYIDMPLQHIDEAMLGRMRRETSRAHIEDLIVRLRAGVPGIALRTTF
                     IVGFPGETEAEFESLLEFIERVRFERLGIFKYSQEDGSRAAQMPGQISAAQKNARQRR
                     AMTLQQKIAREIAEAKVGCALKLLVDQPFIARTEADAPDVDTRVLLAEAAPVGEFVRR
                     KITGARGYDLLA"
     gene            complement(2105..2581)
                     /locus_tag="VNW28_08575"
     CDS             complement(2105..2581)
                     /locus_tag="VNW28_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10023.1"
                     /translation="MSKASAERPSKAQQNCSGTDSANPEGRGPATNGGAARMTPADGP
                     LAGWTLPNRTVAFVGESGVVATRAVSLRGPVCAPGFSVGVFIGNMPGLGDAGRGPDGG
                     GWAGGLGPASDSGAFTSVGEEEDGGLTPAGRGEIFGGRIAPGGTGASGRRMGGAGG"
     gene            2615..2944
                     /gene="trxA"
                     /locus_tag="VNW28_08580"
     CDS             2615..2944
                     /gene="trxA"
                     /locus_tag="VNW28_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HXA10024.1"
                     /translation="MENSAAITIDESNFDQEVTKSAQPVLVDFWAEWCGPCKMIAPIL
                     DEIAKEKAGTVKIAKVNVDHNQSLSFRYNIRAIPTLLFFKDGQVRDQVTGIASKKDLL
                     NRLAALA"
     gene            2972..3103
                     /locus_tag="VNW28_08585"
     CDS             2972..3103
                     /locus_tag="VNW28_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10025.1"
                     /translation="MTSSEYPLPDVVAAVYLTKQQDAGFAATAGGIPAFKINRAREW"
     gene            3087..5510
                     /locus_tag="VNW28_08590"
     CDS             3087..5510
                     /locus_tag="VNW28_08590"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXA10026.1"
                     /translation="MLASGNTVVRFAIAEEMLQIDYSCDDIDRERTVFVALKTDSGSG
                     SAVVPFGEYFEGSTVFLPFQANKLYLLRMGAGTGSAWQRTWENWKWSERREADKEMDF
                     EVGAGGCGIRLPLATLGKKVSVILYCKDFTGNKNWGRLCGAQDPLVVPGEGDKYIPHY
                     CEVDLGARDAPAVKVRGRLGHDGSRPRIYQLFVRLFGNNNETRRPNGSLATNGVGKFN
                     DINDAALASLQKLGISHIWLTGVLQQATGTDYSDIGQPVDDPDLLKGIAGSPYAIKDY
                     FDVCPDYAVDPRKRLAEFKTLLERLHQHRLKALIDFVPNHVARCYHSDIKPEINFGTN
                     DDRAHFFDPRNNFFYLPPNEDGPPLKMPTWQDGVALSPTCKLEGMSCDGRFEGELDHG
                     KVTGNNVVSWKPSLGDWYETVKLNYGFDFTDPAKQRRAYPSAWQPDAPVPDTWTKMDQ
                     VITYWQAMGVDGFRCDMAHMEPPEFWKWLIGRARERSPDVFFMGEAYDSDPAKVPGSD
                     PVISLLHGGRSNVMFDLLDAGFDAVYDDPSYKALKNIYDGPGWANDLDHARSDDFIFE
                     NSVRYAENHDEVRLAGRDHWGGLGMGVGFPVAAILYGIGRGSLMLYNGQEVGEPAEGA
                     EGFGGDDARTTIFDYWSMPELVKWTNGHEYDGARLSPEQRELRAGYGRLLAVTSEAAF
                     CEGAFFGLNPANNQNPKFGRIGMEPAGGHWLYAFLRFDPVSRQRFLVVVNLHPREELR
                     ETRILFPDEAIDFLRFEPNDNKTLHLAERLAGELELEINRSTLAGPDGLALPALAPLT
                     PYFFEISMC"
     gene            complement(5538..7118)
                     /locus_tag="VNW28_08595"
     CDS             complement(5538..7118)
                     /locus_tag="VNW28_08595"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXA10027.1"
                     /translation="MRAKYDVVILGAGHNGLVAAAYLSRAGLSVLLLEKNDYIGGATT
                     SQKIFPDYDAWLSRYSYLVSLFPEKIIRDLNLNLELRRREIASYTPYAKDGKHKGLLL
                     SNVSEEISRRSIFELTGSAADFDGMKRFYGLAAVFAEQVWDTMLSPLAAKEDFRRRFD
                     SSDAGREAWRSLVEEPLGRAVERYLQDDLVRGLVFTDAKIGVFTHPHDESLLQNRCFL
                     YHLIGNKTGEWKVPVGGMGNVARKLEQAARAHGAEMLTRADLRRLELSGKRLVEFECD
                     GQAQAVEARFLLVNFGLNVLAKIRGQPYQPDVTNEGSVFKINMLLHRLPKLKDNSHAV
                     TDAFRGTFHIDEGYEQMKASYRQAAHGQLPEKVPSEIYCHTLTDDSILSPALRTKGFH
                     TLTLFGIDTPWPLFAAENDAMRQRAQARFVAGLNGWLAEPLEDCLAQASDGSLCIESK
                     SPVDIENALGLYHGNIFQTALTYPFAETKEETGKWGVETEYENVYLCGSSAQRGGAVS
                     GIPGHNAAMKVLERVRTS"
     gene            complement(7149..7925)
                     /locus_tag="VNW28_08600"
     CDS             complement(7149..7925)
                     /locus_tag="VNW28_08600"
                     /inference="COORDINATES: protein motif:HMM:NF025175.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4184 family protein"
                     /protein_id="HXA10028.1"
                     /translation="MPWTLSHPAAVLPLRRFTPWPLDFAALVAGSMTPDLGYYIDRFD
                     LSTFAHTLPGSFVACLPTGVIFLLVFYLFCKPFCYALPAPHRQVLLPLCPVFPKGLTR
                     WAIIIFSLLLGTWTHNFWDAFTHEHGWFVDRITWLQQPVLQVPSTTICVYLALQELST
                     VVGFVIVAIAYARWLRRQPVDRSKASESDRWRYLLWAAIVIVSLMISLPPALRIASEF
                     HGFLFYRSLAFQTAIDSPDIAIPLSLLVTTVIYAHRHRRT"
     gene            complement(7939..8886)
                     /locus_tag="VNW28_08605"
     CDS             complement(7939..8886)
                     /locus_tag="VNW28_08605"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXA10029.1"
                     /translation="MVLSIGSNDDIASVVRPDRRPKVVRRAIKNRLGKSRGGGMVLRM
                     ATERDYVLGTHEEEISRLGLQHRVWRPVMLDCWQRAGITVGSRVLDLGAGPGYATADL
                     AEIVGPMGAVVAVERSSNFVRRLEERCHARLFNNVQIRELDLMTDKLPKGPFDFSWCR
                     WVASFVTNPALLVEKLGPVMHPGSIAIFHEYAHYLTWRFVPRLPNQERFAQEVKASWT
                     AAGGKADVGLDLPTYLVQNGFVVRSAIPRIYCLHPGDHMWRWPASFVGIGLARLQELG
                     RIDASFAEKVKKEVAEASANPNALLITPLVLEILAEKVS"
     gene            8907..9161
                     /locus_tag="VNW28_08610"
     CDS             8907..9161
                     /locus_tag="VNW28_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10030.1"
                     /translation="MAAALLGAGMWIHPGSAAVAISINIGDRPYYDGPTYWESGYEWV
                     WVPGYQSGPRWVHGHYERRGDFHAEHAKDHHKHRHPNDRH"
     gene            9453..9965
                     /locus_tag="VNW28_08615"
     CDS             9453..9965
                     /locus_tag="VNW28_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXA10031.1"
                     /translation="MIKFLVGIVVGICLVLLAGYLFVARGGIHMSTAGGSLPMEHYLA
                     QTALSASIGKSAQEQSPLPSDEANLLAGAHIYQQSCGGCHGRLDGKPSGLAKRVYPNI
                     PPLLPPGEGVTDDPVGETHWVIKNGIRFSAMPSFDGKLTENELWQVSLLLRNANKLSA
                     PVQEALRSKE"
     gene            complement(10080..11159)
                     /locus_tag="VNW28_08620"
     CDS             complement(10080..11159)
                     /locus_tag="VNW28_08620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXA10032.1"
                     /translation="MKSDLLLNIADYARAARTKLPQEVCDYYEGGALDEVTLRENTDG
                     WTRLKLYYRVLAGVAARDLRTTVLGQPVSMPILVAPTAFHKLACEAGEKATARAAKAA
                     GTLFILSSLSNTAMELVFAQAGSPRWFQLYVYKDREITRELVQRAEAAGAEAIVLTVD
                     APGLGTRERDARSSFRLPDGLVMENLAPLGKGNFPEVQGSGLAAYARANFKSDLGFDD
                     LDWLCQSTCLPVVVKGVCRGDDARRAAEHGAKAVVVSNHGGRQLDTAPATCEVLPHVV
                     EAAGDLCEIYVDGGIRRGSDVLKALALGARAVLVGRPVLWGLCVGGEDGALQVMEILR
                     RELDEAMLLCGCSKLGDIDRALLQP"
     gene            11315..12337
                     /locus_tag="VNW28_08625"
     CDS             11315..12337
                     /locus_tag="VNW28_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXA10033.1"
                     /translation="MRIVIAAKRLLEKLSVMNKATNTLNGKRLLQKRLLFGLLGAIAL
                     SAVGLLSAESSPSESPPARAASETAGKANPLKPPAQGGIPVAFLVSEGAVIIDFCGPW
                     EVFQDTNVPGRQDAPFRLYTVAEATAPIRASGGMTIVPDYALKDAPAPKVIVIPAQSK
                     PSEAVLAWIRESTKNTDVTMSVCTGAFVLAKTGLLSGKAATTYHGAFRSFAAEFPDIQ
                     LRRGARYVEEGNLASAGGLSSGIDLALRVVERYFGREVAQKTAYDMEYQGKGWMNPDS
                     NQTYAKEPISTMEHPLCPVCQMEVDPATAPKSVYKGKTYYFCSKADKDQFDAAPDKFA
                     DTAKKL"
     gene            12337..13431
                     /locus_tag="VNW28_08630"
     CDS             12337..13431
                     /locus_tag="VNW28_08630"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXA10034.1"
                     /translation="MLLRKPAIRSRRGNCSAKKEQVQTRTRRVLFIAAPGTEILDLVG
                     PLQVFARAADIYRKQDPRSPPIYSVEVITISSRASVVTNCGLRIAAHNTFRRVRGEID
                     TLLIAGGSAIENDEIDLEVVQWIRKVSRCIRRIGSVCTGALLLARAGLLDGRQAATHW
                     KWCELLALKYPRTRVDPNPIFIRDGNVYTSAGVTAGMDLALALVEEDHGSRLALKVAR
                     NLVLYMRRPGGQSQFSAALSMQLTDRKPLLELESWVLDNLNKPLNVPVLAARVGMSPR
                     NFARIFPREMKTTPAKFVERLRVEAARRRLEESRNSLETIAGECGFGNVNSMRNVFQR
                     TLRIAPGQYRHHFRSLRHLPRGAKVRAGNT"
BASE COUNT         2770 a         4125 c         4035 g         2556 t
ORIGIN      
        1 accccgccgc ttggaaacat tgccgcgccc cgcgctcttc cggtggaagg cacgcccccg
       61 ggcgaggcgc gcgtcgcggc gacgccgccg accaccttgg aagcggcgaa gccttccgcg
      121 accgaaccgg aggtacgccg cgcggagctg gtccatcccg aagatttgat cgcgtcacag
      181 acaagcgcca accgcgtgca aattacgccg gtccggaaaa cgtatcttcg cgtgacggtc
      241 gatcgggatc cgcagccggc cttcgacggc tggttggatc cgtccgatcc gccgctctcg
      301 tttcgcgggc ggcacgtgag ggtaaaagtg ctggagcggg gggcggtgca gatcacaaaa
      361 aacggagcgg tgctcgcgcc gggcgatgcc gatgtcacct ttgaatagtg cgcccgaggc
      421 cgaccccagc ctaacgacgg tcgggatgat cagtctcggc tgcgccaaga acctggtcga
      481 cgccgagatc atgctcggga gcgtgcggga gcgggggatg cggatcacgc cgcacgccgc
      541 ggaggccgat gtgctggtgg tgaacacctg cgcgttcatc gactcggcga aagaggaatc
      601 gatcgaagcc attctcgagg cgcatcagca acgcggccta acgagtcggc cggagcaaaa
      661 gctgatcgtg agcggctgca tgtcgcaacg tttcgcgcag gagctgcgtg aacagttgcc
      721 ggaagtcgac gccttcatcg ggctcgatca ggtgcgcgag ctgggcgcga ttatcgagcg
      781 cgtgttgagc gcccagagcg agccgggaga aaatttcgtc accgcacggc cgacctacat
      841 ccccgattgg gacacgccgc gcttccgcct caccccggcg cacagcgttt atttgaaaat
      901 cgccgagggc tgcaatcacc cgtgcagttt ctgtgttatc ccgcagatgc gcggccggca
      961 tcgcagccgg ccgcccgcct cagtccttgc ggagattcgc cgcctcgtgg ccgaaggggt
     1021 gaaggaaatc aacctgatca gccaggatac cacttactac ggaatggact tgtggagcgc
     1081 gaaggccggg ccgcgccagc cggttgactc gtcacgcggt ccgacgctcg cggcgttact
     1141 ccgcgcgatc gaggagatcg agggcgattt ttgggtccgc cttttgtaca cccatccggc
     1201 gcactggagc gacgaactga tcgagacgat cgcgcaatgc ggcaaagtgg cgcgctacat
     1261 cgacatgccg ctgcaacata tcgacgaagc gatgctcgga cggatgcgcc gtgagacttc
     1321 gcgcgcgcat attgaagatc tgatcgttag gctacgcgcc ggggttccgg gaatcgcctt
     1381 gcgtaccact ttcattgttg gattcccggg cgaaacggag gcggaatttg aatcgctcct
     1441 cgaattcatc gagcgcgtgc gattcgagcg actcgggatt ttcaaatact cgcaggagga
     1501 cggctcgcgc gcggcgcaaa tgcccgggca gatttccgcg gcgcagaaga acgcccggca
     1561 gcgacgggcg atgacgctgc aacaaaaaat cgcgcgtgaa attgccgagg cgaaagttgg
     1621 atgcgcgctg aaacttttgg tcgaccaacc gttcatcgcg cggaccgaag cggacgcccc
     1681 ggacgtcgac acccgcgtcc tgctggccga agccgccccg gtcggcgaat tcgttaggcg
     1741 aaaaatcacc ggcgcgcgtg gttacgacct gctggcgtga gcgattggag attgacactg
     1801 cgggcaatgc ctgagagaca cgttgccggc gcagtcgcgg agtccaattt tatccttttc
     1861 ttatggccga tctgagcgaa ccgaaaaaag aaaccgttcg gcttacgtta ccgcaagcgc
     1921 ctgctctgcc ggtggaaaag gaaacaccgc gcagcagctt gccggatcga ccaccgggtt
     1981 cgcccgcggc ggacacatcg ccggaggcga gcgtactggc aacttcgccg gctcccttcc
     2041 gcccgccgcc gccgccatcg gttgcgccaa agccctggcc gcctcccgga ggcgcgttgg
     2101 ctaatcatcc gcctgcgcca cccatgcgcc ggccgctcgc tcccgtcccg ccgggtgcga
     2161 tccgtccgcc gaatatttca cctctccccg ccggcgtcag gccgccgtct tcctcttcgc
     2221 cgacggaagt gaacgcaccg ctgtcgctcg ccgggccaag gccgcccgcc caaccaccac
     2281 catccgggcc gcgcccagcg tcgcccaagc ccggcatatt cccgatgaaa acgccaacgg
     2341 aaaaccctgg cgcgcagact ggcccgcgca aggaaaccgc ccgcgttgct accaccccgg
     2401 attcgccgac gaaagcgacc gtgcggttgg gcaacgtcca gccagcgagc ggtccgtcgg
     2461 ccggggtcat ccgggccgcc ccgccgttag tcgccggtcc gcggccttcg gggttggctg
     2521 aatccgtccc actgcaattt tgctgggcct tgcttggcct ctccgcgctg gccttgctca
     2581 ttcaaatctg gacctatttt tcttaaaaaa gctcatggaa aactccgccg ccataacgat
     2641 cgacgaatca aattttgacc aggaggtaac gaaaagcgcg caacctgtgc tggtcgattt
     2701 ctgggccgaa tggtgcggcc cgtgcaagat gattgcgccg atcctcgacg agatcgcgaa
     2761 ggaaaaagcc ggcaccgtca agatcgcgaa agtgaacgtc gaccacaacc aaagcctgtc
     2821 gtttcgctac aacattcgcg cgatcccgac cctcctcttc ttcaaggacg gacaggtgcg
     2881 tgaccaggtg accggcatag cgagcaagaa agacctgctc aaccgcctcg cggcgctcgc
     2941 ctaaagagcg ggcgcctgcg tcagtcatcc tgtgacctcg tcggaatacc cgctgccaga
     3001 cgtggtggcg gcggtttacc taacgaaaca gcaagacgct ggatttgccg cgaccgcggg
     3061 cgggattccc gcgttcaaga tcaaccgtgc tcgcgagtgg taacaccgtc gttaggtttg
     3121 cgatcgcgga ggagatgctg cagatcgact acagctgcga tgacattgac agggaacgca
     3181 ccgtcttcgt tgccctcaag acagactcgg gaagtggttc ggcggtggtt cccttcggcg
     3241 agtacttcga gggctcgacc gtcttcctgc cgtttcaggc gaataaactc tacctcttgc
     3301 ggatgggagc cgggacggga agcgcctggc agcgaacttg ggagaactgg aagtggagtg
     3361 aacgccggga ggccgacaag gagatggact tcgaagtcgg agccgggggc tgcgggattc
     3421 gtcttccgct cgcgacgtta ggcaaaaagg tcagcgtcat cctctactgc aaagacttta
     3481 ccgggaataa aaactggggc cggctctgtg gagcgcagga tccgttggtt gtgccggggg
     3541 agggcgacaa atacatcccg cattattgcg aggtcgatct cggcgcgcgc gacgcaccgg
     3601 cggtcaaagt ccgcggccgg ctcggtcacg acggaagtcg gccgcggatt tatcaactct
     3661 tcgtcaggct gtttggcaac aacaacgaga cgcgccggcc gaacggtagc ctggcgacga
     3721 acggcgtggg gaaattcaac gatattaacg acgccgcgct cgcctccctg cagaaactcg
     3781 gcatctcgca tatctggctg accggcgttt tgcagcaggc gaccggcacc gattattccg
     3841 acattggcca gccggtcgac gatccggacc tgctcaaggg aatcgcgggc agcccgtatg
     3901 cgatcaagga ttacttcgat gtttgccccg attacgcggt cgacccacgg aagcgcctgg
     3961 cggaattcaa gactctgctc gaacgcctcc atcagcatcg actcaaagcc ctgatcgatt
     4021 ttgttcccaa ccacgtcgcg cgctgttacc actccgacat caagccggag atcaacttcg
     4081 ggacgaatga cgatcgggcg cactttttcg atccgcggaa caacttcttc tatctcccgc
     4141 ctaacgaaga tggtcccccg ctgaagatgc ccacctggca ggacggcgtc gcgctcagtc
     4201 cgacctgcaa gttggaaggc atgagctgcg acgggcgttt cgaaggggag ttggaccacg
     4261 gcaaagtcac gggcaacaac gtcgtcagtt ggaaaccaag tctcggcgat tggtacgaga
     4321 cggtgaaact aaactacggc ttcgatttca ctgaccccgc caaacagcgg cgcgcctatc
     4381 ccagtgcctg gcagcccgac gcgccagtcc ccgatacctg gacaaaaatg gaccaggtca
     4441 tcacctactg gcaggcaatg ggagtcgatg gctttcgctg cgacatggcg cacatggaac
     4501 cgcccgaatt ttggaaatgg ctcatcggcc gggctcgcga gcgttcgcct gacgttttct
     4561 tcatgggcga agcctacgac agcgatcccg cgaaagtccc gggcagcgat ccggtcattt
     4621 ccctgctcca cggtggcagg tcgaacgtca tgttcgacct cctcgacgcc ggcttcgacg
     4681 ccgtctacga cgacccgagc tacaaggcgt tgaagaacat ctacgacggc ccgggctggg
     4741 ccaacgatct cgatcacgcg cgctccgacg atttcatttt cgaaaattcg gttcgttatg
     4801 ccgagaacca cgatgaagtc cgtctcgccg gtcgcgacca ttggggcggc ctcggcatgg
     4861 gggtcggatt tcccgtggca gccatccttt acggaatcgg gcgcggctca ttgatgctct
     4921 acaatggaca ggaagtcggc gagccggcag agggcgctga gggattcggt ggcgacgacg
     4981 cgcgcacaac aatcttcgat tactggtcga tgccggagtt ggtgaagtgg actaacggac
     5041 acgaatacga cggcgccaga ctttcccccg agcagaggga attacgcgcg ggatacgggc
     5101 gcctgcttgc cgtgaccagc gaggcggctt tctgcgaagg ggcttttttc ggtttgaatc
     5161 cggcaaacaa ccaaaatcca aaatttggcc ggatcggaat ggaaccggcc ggcggccatt
     5221 ggctctacgc gttcctccgt tttgatccgg tctcgcggca gcgcttcctc gtcgtggtga
     5281 atctgcatcc gagagaggag ttgagggaaa cgcgcatcct ttttcccgac gaggcgatcg
     5341 acttcctgcg ctttgaacct aacgacaata agactcttca tctcgctgaa cgcctcgcgg
     5401 gcgagctcga gttggagatc aaccgctcca cgctggccgg cccggatggc ctggcgctcc
     5461 cggcactggc gccgctgact ccctactttt ttgaaatcag tatgtgttaa ggggcgataa
     5521 tgcccgcgcg cagtgcccta ggacgtcctg acccgctcaa gcactttcat ggcggcgttg
     5581 tgaccgggga taccgctgac cgctccgcca cgttgggcgc tggagccgca gagatagaca
     5641 ttttcgtact cggtctcgac cccccacttt cccgtctcct cttttgtctc ggcaaaagga
     5701 taggtgagcg cagtctggaa gatattaccg tgataaagac cgagcgcatt ttcgatgtcg
     5761 accgggcttt tgctttcgat gcagagactt ccatcgctgg cctgcgccag gcaatcctcc
     5821 agcggctctg ccagccaccc attcagaccc gccacgaatc gcgcctgagc ccgttgcctc
     5881 atcgcatcat tctccgcggc aaagagcggc caaggagtat caatcccgaa aagagtcagg
     5941 gtatgaaagc cttttgtgcg cagtgcggga gacaaaatgc tgtcatcggt cagggtgtga
     6001 caataaattt cgctgggcac tttctccggc agttgaccgt gcgccgcctg ccgatagctc
     6061 gctttcattt gctcatagcc ttcgtcgatg tggaaagtgc cgcgaaaagc gtcggtcacc
     6121 gcgtgcgagt tatccttcag ttttggcagg cggtggagca gcatgttgat cttgaagaca
     6181 gagccttcat tcgtgacatc gggttggtag ggttggccgc ggattttcgc cagcacattc
     6241 aaaccgaagt taaccagcag aaatcgcgct tcgaccgcct gcgcttgacc gtcgcactca
     6301 aattcgacga gccgcttccc cgaaagttcg agacggcgaa gatcggcgcg cgtcaacatc
     6361 tcggcgccgt gcgcgcgcgc ggcctgctcc agcttgcgcg cgacgttgcc catcccgccg
     6421 accgggacct tccattcgcc agtcttattg ccgatcaggt gataaaggaa acaacggttc
     6481 tggaggagtg actcatcgtg cggatgagtg aagacaccga tcttggcgtc cgtgaaaacc
     6541 agcccgcgta ccagatcgtc ctggaggtaa cgttcaacgg cgcgtccgag cggctcctcg
     6601 accaggctgc gccaagcctc acgcccggcg tcgctcgagt cgaagcggcg gcggaagtct
     6661 tccttcgctg cgagcggcga taacatcgtg tcccaaacct gttcggcaaa aacagccgcc
     6721 aggccgtaaa accgcttcat cccgtcgaag tcggctgcgc tgccggtcaa ttcgaatatc
     6781 gaccgtcgac ttatttcttc cgaaacgttg ctcaggagca aacctttgtg ctttccgtcc
     6841 ttggcgtacg gcgtatagga agcgatctcc cggcggcgca attcgagatt caggttcagg
     6901 tcgcgaatga ttttttccgg aaaaaggctg accagataag aataacgcga gagccacgcg
     6961 tcatagtcgg gaaagatttt ttgcgaggtc gtggctccgc cgatgtaatc gttcttctcc
     7021 aacaaaagga cacttagtcc cgctcgcgaa aggtaggcgg cggccaccag tccgttatgg
     7081 ccggctccca ggatgacaac atcgtatttt gctctcatgc tcgagttttg gctttggatt
     7141 ctagccggtc aggttcttcg gtggcggtgc gcataaatga cggtggtgac aagcagactc
     7201 aaagggatgg cgatgtcggg agagtcaatc gccgtctgaa aggcgagcga gcgataaaaa
     7261 aggaagccat ggaactctga cgcgatgcga agggcgggcg gcagcgaaat catgagcgat
     7321 acgatgacaa tggccgccca aagcagatag cgccagcgat cggattccga ggccttcgac
     7381 cgatcgacgg gttggcggcg gagccatcgc gcataagcga tggcgacaat cacaaatcct
     7441 acgacggtgc tgagttcctg aagagcgagg tagacgcaga tggtcgtcga ggggacctgc
     7501 aaaactggct gttgcagcca ggtgatgcgg tcgacaaacc aaccatgttc atgggtgaag
     7561 gcgtcccaga aattatgagt ccatgtcccg agtaagaggg aaaaaataat gatcgcccac
     7621 cgtgtcaggc ccttgggaaa aactgggcaa agcggcagga gcacctgccg gtgaggcgcg
     7681 ggaagggcat aacaaaacgg cttgcaaaac aggtagaaga cgagcaggaa aatcactccc
     7741 gtaggcaaac aggcgacgaa acttcccggc aaagtgtggg caaaagtcga cagatcaaag
     7801 cggtcgatgt agtagccgag atccggtgtc atgctcccgg cgacgagggc ggcgaagtcg
     7861 agcggccacg gagtgaagcg ccgcaggggt aggacagcgg caggatgaga aagcgtccag
     7921 ggcatcccag cgcgattctt aggaaacctt ctcggcgagg atttccagga cgaggggcgt
     7981 aatcaacaac gcgttgggat tcgcactcgc ctcggccact tccttcttca ccttctcggc
     8041 aaagctggca tcgatgcgtc ccagctcttg cagccgcgcc agacctatcc cgacgaatga
     8101 tgccggccag cgccacatgt ggtcacccgg atggaggcaa taaatccggg gaatggctga
     8161 gcgaacgaca aagccgttct gcacgagata ggtcggcaga tccaggccga catcggcttt
     8221 ccctcccgcc gcggtccaac tcgccttcac ctcctgcgcg aagcgctcct ggttcggcag
     8281 ccgggggacg aagcgccagg tgaggtaatg cgcatattca tggaagatcg cgatacttcc
     8341 cgggtgcatc acgggcccga gcttctccac gagcagtgcc ggattggtca cgaacgaagc
     8401 cacccagcgg caccaggaga aatcgaatgg acccttgggc aacttgtcgg tcattagatc
     8461 cagttcgcga atctggacgt tgttgaataa gcgggcatgg cacctttctt ccagccggcg
     8521 cacgaaatta ctcgagcgtt cgaccgcgac gaccgcgccc atcgggccca cgatctcagc
     8581 caaatcggcc gtcgcataac cgggcccggc gccgaggtcg agcacgcggc ttccgaccgt
     8641 gatgccggct ctctgccagc aatccaacat gacgggacgc cagacccgat gctgcaaccc
     8701 gagccgggaa atctcttctt cgtgtgttcc gaggacgtaa tcgcgttccg tggccatgcg
     8761 cagaaccatt ccaccgccgc ggctttttcc cagacgattt tttatcgccc gacgaacgac
     8821 cttcggccgg cggtccggcc ggacgaccga agcgatgtcg tcattcgaac caatgcttaa
     8881 caccatgaaa aagacgaatt atcacgatgg cagcggcgtt actcggggca gggatgtgga
     8941 ttcatccggg ctcagcggcg gtcgcgatct caattaacat cggcgatcgg ccttactacg
     9001 atgggccgac ttattgggaa agcggttacg aatgggtctg ggtgcccggc tatcaaagcg
     9061 gccccagatg ggtccacggg cactatgagc gccggggcga cttccacgcc gagcacgcca
     9121 aggatcatca taagcatcgc caccccaacg accggcacta atcagcgcgt cagaagaagc
     9181 cagaccgctg gcgtggagcc tggactcgcc gcggcgagtc tctcggaggg gcgcgcctcc
     9241 gcgcgtccag cctcccgccc ttgaattgcg actcaactct tttgattatg ctgcgccagg
     9301 aggtggcttc ccgcgcgaga acgcatgctg tttgctccgg aacaactgaa gatgcgcgtc
     9361 cgaaagcggt cgcgtttcgc gcggtcgcaa gttctgtgcg atgatcccga cgcgcgctgt
     9421 tacggtatct cattccgtcg ccgcattttc ctatgattaa attcctggta ggcatcgtgg
     9481 tcggaatctg tctcgttctt ctggcaggtt atctttttgt ggcgcgcggc ggcattcaca
     9541 tgtcgaccgc cggcgggtcg ttgccgatgg agcactatct cgcgcagacg gccctatcgg
     9601 cctcgatcgg caagagcgcg caagagcaat ccccgctgcc gtccgacgag gccaacctcc
     9661 tcgccggcgc gcacatttat cagcaaagct gcggcggctg ccacgggcga ttggatggga
     9721 aaccctcggg actagcgaag agagtttatc caaatattcc gccgcttctt cctccgggcg
     9781 agggtgtgac tgacgatccg gtcggagaga ctcactgggt gataaagaat ggcatccgct
     9841 ttagcgcgat gccttccttt gatggaaagc tgaccgagaa cgagctttgg caggtaagtc
     9901 tacttttgcg taacgccaat aagttgtccg cgccggtgca ggaggcgctg cgcagtaagg
     9961 aataggtcag gatcgacgcg actactctgc gccgggggat gagcttccgc ccagcgacct
    10021 ttcctccggg gctggcgcgc ttggcgcgcc gctcccggcg cctcgccggg aggaagtttt
    10081 caaggctgca acaacgcccg gtcgatatcg cccagcttcg agcagccgca aagcagcatc
    10141 gcttcgtcga gttcgcgtct caggatttcc atcacttgca aggcgccatc ttctccaccg
    10201 acgcacaatc cccacagcac cggtcggccc accaacaccg cccgcgcccc gagcgcaagc
    10261 gccttgagca catcgctccc tcgacgaatt ccaccatcga cataaatttc gcaaagatcg
    10321 cccgccgcct ccaccacgtg cggcagcacc tcgcaggtgg cgggcgcggt atcgagctgg
    10381 cgtccgccgt gatttgaaac caccaccgct ttcgcgccgt gctcggctgc ccggcgcgca
    10441 tcgtcgccgc ggcacacgcc cttcaccacc accgggaggc aagtggactg gcataaccaa
    10501 tccaggtcat cgaacccgag gtcggatttg aaatttgctc gcgcataggc ggccaggccg
    10561 gatccttgca cctccggaaa attgcccttg cctaacggcg ccagattttc catcaccagg
    10621 ccgtccggca ggcgaaagct gctccgcgca tcgcgttccc gcgtacccag gcctggcgca
    10681 tcgaccgtca agacgatcgc ctccgcccca gccgcctcgg cccgctgcac cagttcgcgc
    10741 gtgatctccc gatctttgta aacatagagt tggaaccagc gcgggctgcc cgcctgcgca
    10801 aagaccaact ccatcgcagt gttcgagagc gaactgagaa tgaacaaagt cccagccgcc
    10861 ttcgcggccc gcgcggtcgc cttctctccc gcttcgcagg ccagtttatg aaaagcggtc
    10921 ggggcgacga ggatcggcat cgagaccggt tgaccaagca cggtcgtgcg caggtcgcgc
    10981 gcggcgactc cggccagaac ccgatagtaa agcttcaggc gcgtccagcc atccgtgttc
    11041 tctcggagcg tcacttcatc cagcgccccg ccctcatagt agtcacacac ttcctgcggg
    11101 agtttggtcc gcgccgcccg tgcgtagtcg gcgatattga gcaggagatc ggatttcatc
    11161 ttaggacgat gacaagatgc tcccccagta tgccgtcaac tgaatttcca ctttttgcta
    11221 tctaatttcc gccaggcgtc cggccgcggg acgcagcacc ggactgcttt cggggaaagc
    11281 cttttttctc ccggaaagat gtggcagtaa tatgatgaga attgtcattg ctgccaaacg
    11341 cttgctggag aagctgagtg tcatgaataa agcgacaaat accttgaatg gaaaaaggtt
    11401 gctccagaaa agattgctct tcggtttgct gggcgcgatt gctctttccg ctgttggcct
    11461 gctttccgcg gagtccagcc cgtctgaatc cccgcctgct cgcgccgcga gcgaaacagc
    11521 cggcaaagcg aatccactca aacctcccgc ccaaggcggt atccccgtag cctttctcgt
    11581 ttcggaaggc gccgtgatta ttgatttctg cgggccgtgg gaagtcttcc aggatacaaa
    11641 cgtccccggg cgccaggatg ctcccttccg tctttacacc gtggccgagg cgaccgctcc
    11701 cattcgcgca agtggcggga tgacgattgt ccccgactac gcgctaaaag atgctcctgc
    11761 gcccaaagtc attgtcattc cggcacaaag taagccgagt gaggcggtgc tggcatggat
    11821 tcgcgaatcg acaaaaaaca ccgatgtgac catgtcggtc tgcacgggtg catttgttct
    11881 ggccaagacc ggcctcttgt cgggcaaagc cgcgaccaca tatcacggag cgtttcgcag
    11941 ctttgccgcg gagttccccg atatccagct caggcgcgga gcccgctacg tcgaagaagg
    12001 taatctcgcg agcgccggcg ggctttcctc ggggatcgat ctcgcgctgc gcgtggtcga
    12061 gcgttacttc ggccgcgagg tcgcgcagaa gacggcctac gacatggaat accagggtaa
    12121 gggctggatg aatccggatt caaatcagac ttacgccaag gaacccatct ccacgatgga
    12181 gcatccactc tgcccggtgt gccagatgga agttgacccg gcaaccgctc cgaagtcggt
    12241 ctacaaaggc aagacttact acttctgttc caaggcggac aaggaccagt tcgacgccgc
    12301 tcccgataag ttcgcagaca ccgctaagaa gctttgatgc tactcaggaa acctgccatt
    12361 cgatcgagaa gagggaactg ttcagccaag aaggagcagg ttcaaacgcg gacgcggcgg
    12421 gtgctgttca ttgcggcgcc cgggaccgag atactcgatt tggtcggacc attgcaagtc
    12481 tttgcccgtg cggcggacat ctaccgcaag caagaccccc ggtcacctcc gatctattcc
    12541 gtcgaagtca taaccatctc ctcccgggcc tcggttgtca caaactgtgg cctacgcatc
    12601 gcggctcata acacgtttcg gcgggtgcgc ggcgagatcg acaccttgct catcgccggc
    12661 gggagtgcga tcgagaacga tgagatagac ctggaggtgg tgcaatggat ccgaaaggtg
    12721 tccagatgta ttcggcgtat cggctccgtc tgcaccggcg cgctgctcct ggctcgagcc
    12781 ggcctgctcg acggccgcca ggcggcaacc cactggaaat ggtgcgagct tctcgccctg
    12841 aagtatccgc ggaccagggt tgatcctaat ccgatattta tccgcgatgg gaatgtctac
    12901 acatccgccg gggtgacagc aggcatggat ctggccctgg ctctcgtgga agaagaccat
    12961 ggttcgcggc tggcactaaa ggtggcgcgc aacctcgtcc tttacatgcg ccggccagga
    13021 gggcagtccc aatttagcgc cgctctctcc atgcaactga ccgatcgaaa gcccttgctc
    13081 gagttggagt catgggtgct cgacaaccta aacaagccgc tgaatgtgcc ggtattggcg
    13141 gcgcgggtag ggatgagtcc tcgcaatttt gcccgaatat ttcccagaga gatgaagacc
    13201 acgcccgcga agtttgttga gcgtttgcgc gtcgaagcag cgcgccgccg cctggaagaa
    13261 agtcgaaaca gcctggaaac gattgctggc gagtgtggct ttgggaatgt caattcgatg
    13321 cgcaatgttt tccagcgaac gctccgaatt gctcccggac agtatcgtca ccactttcgc
    13381 agtctgcgcc acctgccgcg gggagcaaag gtcagggccg gcaacacctg aaccccccgt
    13441 cacaatggcg accttttttc agtggccgcg attggtgctc gcattc
//
LOCUS       DATMNS010000245         2299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3425010, whole genome shotgun sequence.
ACCESSION   DATMNS010000245 DATMNS010000000
VERSION     DATMNS010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3425010"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VNW28_08635"
     CDS             <1..792
                     /locus_tag="VNW28_08635"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HXA10035.1"
                     /translation="EPDVGAAFLARLDEIRAQIDALLATGDQGRIFREGVRVVIYGPT
                     NAGKSSLLNRLLGFPRAIVSDLPGTTRDTIEEMVKLRGIAFRLTDTAGLRTGETDAVE
                     NEGIARTERSLASADLLLQVVDASAAKPAYFVARPADRAALLLLNKSDLAEHEDWKNC
                     DALRISCLAQDGLSGLEDAILSKVSEQRWDVPSAVAINTRHRDCLRRARGACEQARSA
                     FVQNLAPELVAVDLRGALQAVGEVIGHADMEQILDALFATFCIGK"
     gene            789..1820
                     /locus_tag="VNW28_08640"
     CDS             789..1820
                     /locus_tag="VNW28_08640"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HXA10036.1"
                     /translation="MKSRASGCYQRLVRPLLFRMDPEAAHHFALRLLQAAPSIPGSRA
                     LLRLFAPVPRSVTLFGLNFPNPVGLAAGFDKNGVAIPAWEALGFGFVEIGTVTAQPQP
                     GNPRPRIFRYPKQEALINRLGFNNDGADAVAQRLRKLRQSRLRPSIPIGVNLGKSKAT
                     PLEEAAADYHYSFRQLHSVADYIVLNVSSPNTPGLRTLQEHDALVALLRVITDENTRL
                     PAPRPILLKIAPDLSDEALTEIVAAGEEFGLAGLIATNTTLDHSAVVGEGDQSGGLSG
                     DPLRQRSTDVVRFLRSRTHLPIIGAGGIGDRASAQEKLQAGAQLIQVYTGYIYRGPSL
                     LREIIAATS"
     gene            1935..>2299
                     /locus_tag="VNW28_08645"
     CDS             1935..>2299
                     /locus_tag="VNW28_08645"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXA10037.1"
                     /translation="MRNLLPKILAAVFFVLLAGELSAQTGSLTLAWNANPENNIQGYL
                     MDAGTTSGVYDREVDVHDVTTVTVPNLTVGTTYYFVVKAYNTEGLVSLPSAEVSAVAP
                     APSPTPTPTPTPTPTPTPTP"
BASE COUNT          414 a          737 c          710 g          438 t
ORIGIN      
        1 gagcctgacg tgggcgccgc ttttcttgcg cgactcgacg aaatccgcgc gcagatcgac
       61 gcgctgctcg ccactggcga ccagggccgc atctttcgcg agggcgtgcg ggtcgtcatc
      121 tatggcccga cgaatgcggg aaaatcgagt ctgctcaacc gcctgctcgg ctttccgcgc
      181 gctatcgtga gcgacttacc gggcacgact cgcgatacga tcgaggaaat ggtcaagctc
      241 cgcgggattg cattccgcct gacggatacg gcggggctgc gcaccggtga gaccgatgcg
      301 gttgaaaacg aaggcatcgc gcggaccgag cgctcgctcg ccagcgctga tctcctgtta
      361 caggtggtcg atgcgagcgc cgccaagccg gcgtatttcg tcgcgcggcc ggccgatcgc
      421 gccgcactct tgctcctgaa caagagcgac ctggcggaac atgaagattg gaagaattgc
      481 gacgcactgc ggatttcgtg ccttgcgcag gatggcctct ccgggctgga ggacgcgatt
      541 ttgtccaagg tgagcgaaca acgctgggat gtgccgagcg cggtcgcgat caacacgcgg
      601 catcgcgatt gcctgcgccg cgcgcgcggc gcctgcgagc aggcccggag cgcctttgtc
      661 cagaatctgg cgccggaatt ggtggcggtt gatctgcggg gcgcgctcca ggcggtcggg
      721 gaggtcatcg gccacgccga catggagcag atcctggacg cgctcttcgc caccttctgc
      781 atcggaaaat gaaaagtcgg gcaagcggtt gctaccagcg cctcgttagg ccgcttcttt
      841 tccggatgga cccggaggcg gcgcatcatt ttgccctgcg cctcctgcag gcggctccgt
      901 ccattcccgg cagccgcgcg ctgttgcgtc tgtttgcccc ggtaccgcgg tctgtcacgc
      961 tcttcggttt gaattttcca aacccggtcg gtctcgccgc cggcttcgac aaaaacggcg
     1021 tggccattcc ggcctgggag gcgctcgggt ttggctttgt cgaaatcggc accgtcaccg
     1081 cccaacctca gccgggcaat ccgcggccgc gcatttttcg ttacccgaag caggaagcgc
     1141 tgattaaccg gctcggcttt aataacgacg gcgcggacgc cgtcgcgcaa cgcttgcgga
     1201 agctgcgcca gagccggctc cgaccgtcga tcccgatcgg cgtcaacctc gggaagtcga
     1261 aggcgacgcc gctcgaggaa gccgccgccg attaccacta cagcttccgg cagctccatt
     1321 ccgtcgccga ttacatcgtg ctcaacgtca gctcgccaaa tacgccaggc ttgcgcactc
     1381 tgcaggaaca cgacgcgctg gtcgcgctgc tccgtgtcat cacggatgaa aatacgcgct
     1441 tgccagcgcc caggccgatc ctgctgaaaa tcgcgcccga tctttccgac gaagcgctga
     1501 cggaaatcgt cgcagccggc gaggaattcg gtctcgccgg attgatcgcc acaaatacca
     1561 cgctcgatca ctccgccgtg gtgggcgaag gggaccagtc cggcggcctg agcggcgatc
     1621 ccttgcggca acgttcgacc gatgtcgttc ggtttctgcg cagtcggacg catttgccga
     1681 tcatcggtgc tggcggaatc ggcgatcggg cgtccgcgca ggagaagttg caagccggtg
     1741 cgcaactcat tcaggtctac accggctaca tctatcgcgg tccgtcgctc ctgcgggaaa
     1801 tcatcgcagc gacatcgtag cggcggtcgg cgaggaaaga ttttcggcgg gtcgcgccga
     1861 gggtcggcac gttttccagc ccgtcttgcg gcatggacat tgctgtaatt gtcataacta
     1921 ccgtgtttac tggaatgaga aacttgttgc caaaaatcct tgccgccgtg ttctttgtgc
     1981 tcctcgccgg tgaactgtca gctcaaaccg gaagcctcac cctggcttgg aacgccaacc
     2041 cggagaataa tatccaaggc tatctgatgg acgcgggaac gaccagcggt gtctatgacc
     2101 gcgaggtgga cgtccatgat gtcacgaccg tgacggtccc gaatcttacc gtggggacca
     2161 cgtattattt cgtggtcaaa gcctacaaca cggagggcct ggtgagtctt ccgtcggcgg
     2221 aagtctctgc ggttgcgccc gcaccgagtc caacacccac acccacgccc acgccaactc
     2281 caaccccaac tccgactcc
//
LOCUS       DATMNS010000246         5963 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3773272, whole genome shotgun sequence.
ACCESSION   DATMNS010000246 DATMNS010000000
VERSION     DATMNS010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5963
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3773272"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1650
                     /locus_tag="VNW28_08650"
     CDS             223..1650
                     /locus_tag="VNW28_08650"
                     /inference="COORDINATES: protein motif:HMM:NF020390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 domain-containing protein"
                     /protein_id="HXA10038.1"
                     /translation="MPQPAEKWSFESAAHLLNRAGFGGTPEEIEAAHAAGLQAAVYNL
                     LHPPNEPGDNGTHPWTVPEDVRARMREVRKLMAQPDEKRQKLHALRREQRDEMLDLRT
                     WWLGRMKETRAPLREKITLFWHGHFATSAQKVTNVYWMWRQNDTLRQNALGNFNTLLH
                     EMSRDPAMMVYLDLAQNRKAHPNENWAREVMELFTLGIGHYSETDVRESARAFTGYEI
                     NLTNQQFRFNPAQHDASRKSFMGRIGDFSGDDILDIIVRQPACPEWIGRKLWRFFVED
                     EPSPQMVGAVADSLRENHFELRPVLREIFSSAEFYSARIIRSQIKSPVQFLVQSCKLL
                     ETELPPRGIAQNALKQMGQILLTPPNVKGWDGGKSWISTSTLLFRYNFANYLVNGTAN
                     QPNVPPMLQRAPLDPTKIIPGALRDKPEDLIAHLGRRLYQAPLDGKQTETFLAYLKTR
                     APDRGDETMRRLLHLMMSTPQYQLT"
     gene            1663..2982
                     /locus_tag="VNW28_08655"
     CDS             1663..2982
                     /locus_tag="VNW28_08655"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HXA10039.1"
                     /translation="MNQTLQTRRRFLRTSFLGAAASWTLPVFLEKTFLTLDAIAADSL
                     TQTATGKDGTILVVLQLAGGNDGLNMVVPFADDAYFRARPKLALPADKILRLNSYAGL
                     NPKLTGLKSLFDEGHLAVIQGVGYPNPNRSHFRSTEIWQTATDSDRTEREGWLGRYFD
                     NCCRGSDPTVGVAIGSEMPQSFTARTPTGVVFSRPEQFRFKSGEKTGGRMSEEEIFYR
                     QLNQPNDTTITASDGGSIGAIGGATSSDLSAIDFLQRTALDAQISSDKILEIARKSKS
                     TVPYPSGQLANSLSLIARMIGGGLTTRVYYASQGGFDTHARQLNAHDRLMTELNGALS
                     AFVTDLKQQGNFERVLLITFSEFGRRVNENANGGTDHGAAAPMFALGGRVKPGLFGKY
                     PSLTDLDSGDLKFNTDFRSVYGTVLEQWLAAPSAAVLGRKFPPLPII"
     gene            complement(3001..4005)
                     /gene="aroF"
                     /locus_tag="VNW28_08660"
     CDS             complement(3001..4005)
                     /gene="aroF"
                     /locus_tag="VNW28_08660"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF006421.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXA10040.1"
                     /translation="MLIVMTARATEAELAGVVRAITQLGFRAHPMPGAIRTVIGLTGN
                     TGAVDPAHFENMPGVAEAIAISKPYKLVTREFRPEGTVIEIGDAKIGDGGLAIIAGPC
                     AIESRAQAFAIAESVQKSGARFFRGGAFKPRSSPYAFAGLGEEALEIMAEIRERFGLK
                     IVTEAVDEAGLEMVERFADMIQIGARNMQNFSLLRRAGRSRRPVLLKRGLAATLEEWL
                     LAAEYIMVEGNYQIVLCERGVRTFTEHARSVLDLAAVPAVRRISHLPVIVDASHATGR
                     KYLVTPLARAGVAVGADGLMVEVHDHPERALSDGAQALELDEYEQMVRAVRAIHDALA
                     "
     gene            complement(4127..5218)
                     /gene="prfB"
                     /locus_tag="VNW28_08665"
                     /pseudo
     CDS             complement(4127..5218)
                     /gene="prfB"
                     /locus_tag="VNW28_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            complement(5310..>5963)
                     /locus_tag="VNW28_08670"
     CDS             complement(5310..>5963)
                     /locus_tag="VNW28_08670"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10041.1"
                     /translation="AEHRFVGVKSGLLDQVTSIFGRAQHALFFDARSEEVRTIPFPPE
                     LSLVIAESGTKRELAEGLYDQRCEETRAAAAALGLVALRDISPNELAKRDLPPLLRRR
                     AAHIVGENERVQRALILLAENDARGCGALMNESHESSRQNFENSTAELDLLVEIARDL
                     PGVYGARLTGGGFGGATVTLCENVASAGIADELAQTYNRRFGLKARLFICAVADGAR"
BASE COUNT         1147 a         1946 c         1748 g         1122 t
ORIGIN      
        1 tcgagcaaga gggagagcgg tcgcgcgccc gctacccaga tgccgagcaa cgcaatcgag
       61 cccgcgatcc ccagcgcact cctggccaag attcgcatcc cgtgaagtca tatccgccac
      121 ggcgcgagtc gtccatgccg atctcgaatc tccgcgacgg cggagtgtct cacctttgta
      181 accggaccct cttcccgccg gtcatctgtc gaaaccatgc tgatgccaca accagcggag
      241 aagtggagtt tcgaaagcgc ggcgcacctc ttgaatcgcg ccggtttcgg cggaacgccg
      301 gaggagatcg aagcggccca tgcggccggc ctgcaggcgg ccgtttacaa tttgctccat
      361 ccccctaacg aaccggggga caatggaacc catccttgga ccgtccccga ggacgtgcgg
      421 gcgcggatgc gggaagtgcg aaagctgatg gcgcaaccgg acgagaaaag acagaagctt
      481 cacgcgctgc gacgcgagca acgggatgag atgctcgact tgcgcacctg gtggctcggg
      541 cggatgaagg agacgcgcgc gccgctgcgc gagaagatca cgctcttctg gcatgggcac
      601 ttcgcgacca gcgcgcagaa ggtgacgaac gtctactgga tgtggcgcca gaacgacacc
      661 ctccggcaaa acgcgctcgg gaatttcaac accctgctcc acgaaatgtc gcgcgatccg
      721 gcaatgatgg tttacctcga tctcgcgcaa aacaggaagg cgcacccgaa cgagaactgg
      781 gcgcgcgagg tgatggagct tttcactctc gggattggcc attacagcga aaccgacgtg
      841 agagaatcgg cccgcgcctt taccggctat gaaatcaacc tgacgaatca gcaattccgc
      901 ttcaatcccg cccagcatga tgccagccgg aagagtttca tggggcgcat tggcgacttc
      961 tcaggcgacg acatcctcga cataatcgtc cgccaaccgg cctgcccgga gtggattggg
     1021 cggaaactct ggcgcttttt cgtcgaggac gaaccttcac cgcagatggt cggcgcagtc
     1081 gccgattcgc tccgggaaaa tcacttcgaa ttgcgtccgg tcttgcgcga gatttttagc
     1141 tccgccgaat tctattccgc tcgtatcatc cgctcgcaaa tcaagagccc ggtgcaattt
     1201 ctggtccaga gttgcaaact gctcgagacc gagttgccgc cgcgcgggat cgcgcaaaac
     1261 gcgctcaaac aaatggggca gatccttctc acgccgccga acgtcaaagg gtgggacggc
     1321 ggtaaatcat ggattagcac ctcgacactc ctttttcgct ataactttgc caactacctg
     1381 gtcaatggga cggccaacca gcccaatgtc ccgccgatgc tgcaacgcgc gccgctcgac
     1441 ccgacgaaga tcatccccgg ggcgctgcgc gataaaccgg aggacttgat cgcccatctg
     1501 ggccgccggc tctaccaggc accactcgac gggaaacaga ctgagacctt tctggcctac
     1561 ctgaaaactc gcgcgccgga tcgtggcgac gagacgatgc gccgtttgct ccacctgatg
     1621 atgagcacgc cgcagtacca attgacctaa cgcctaacga aaatgaacca aaccctgcag
     1681 acccgccgtc gctttctccg cacttccttt ctcggcgcgg ccgcttcctg gacgctgccg
     1741 gttttcctcg agaaaacctt tttgaccctc gacgcgatcg cggccgattc cctcacccag
     1801 accgcgacgg gcaaggatgg caccatcctt gtcgtgctcc aactggccgg cgggaacgac
     1861 ggtttgaaca tggtcgtacc ttttgctgac gatgcttatt tccgcgcgcg gccgaaactc
     1921 gctcttccgg cggacaaaat cctcaggctc aattcctacg ccggactgaa tccgaagctc
     1981 accggcctga aatccctttt cgacgaaggc cacctcgcgg tcatccaagg cgtcggttat
     2041 cccaatccga accgctccca ttttcgctcg accgaaatct ggcagaccgc caccgactcc
     2101 gaccggacgg agcgcgaagg ctggctcggc cgttacttcg acaactgttg ccgcggatcc
     2161 gatccgacgg tcggcgtggc gataggaagt gagatgccgc agtccttcac cgcgcgcacg
     2221 ccgaccgggg tggttttctc gaggccggaa cagtttcgct tcaagagcgg cgagaaaacc
     2281 ggcgggcgga tgagcgaaga ggagattttt taccgccaat tgaaccaacc taacgacacc
     2341 acgattaccg cgtcggacgg cgggtcgatc ggcgcgattg ggggcgccac ttcgtcggat
     2401 ttgagtgcga tcgattttct gcagcggacc gcgctcgacg cgcaaatcag ctccgacaaa
     2461 atcctcgaga tcgcccgcaa atcgaaatcg accgttccct acccatccgg ccaactcgcc
     2521 aatagcctga gcctgatcgc gcgcatgatc ggcggcggcc tgacgactcg cgtctattat
     2581 gccagccagg gcggatttga cacgcacgcg aggcagttga atgcacacga tcgcctgatg
     2641 acggagctga atggcgcgct gagcgctttc gttaccgatt tgaaacaaca gggaaatttt
     2701 gagcgcgtcc tcctgatcac cttcagcgaa tttggccggc gcgtgaacga gaacgcgaac
     2761 ggcgggaccg accatggcgc ggccgcgccg atgtttgcgc tcggcggccg ggtcaaaccc
     2821 gggctcttcg ggaaatatcc aagcctgacg gatctggata gcggcgacct gaaattcaac
     2881 acggattttc gcagcgttta cggcaccgtc ctcgaacagt ggctcgcggc accgagtgcc
     2941 gcggtgctgg ggcgaaaatt cccgcccctg ccgattattt aggcggcggc ccgagcaacg
     3001 tcaggcaaga gcgtcgtgaa tggcgcgcac tgcgcgcacc atttgttcgt attcgtccag
     3061 ttcgagcgcc tgcgcgccat ccgacagggc gcgctccggg tggtcgtgca cctcgaccat
     3121 caaaccatcc gcgcccaccg ccacgccggc acgggccagc ggcgtgacca ggtatttcct
     3181 tcccgtggcg tgcgaggcat cgacgatcac cgggagatgt gaaatccggc gcacggccgg
     3241 cacggcggcg agatcgagca cgctccgggc gtgttcggtg aaggtgcgca cgccgcgctc
     3301 gcaaagcacg atctggtaat taccctccac catgatgtat tcggcggcga gcagccattc
     3361 ctccaacgtt gcagccaggc cacgtttcag gagcaccggg cggcgcgaac ggccggcgcg
     3421 tcggagcagg gagaaattct gcatgttgcg cgcgccgatc tggatcatgt cggcgaagcg
     3481 ctcgaccatt tccagccccg cctcgtccac cgcttcggtc acgatcttca agccaaagcg
     3541 ttcgcggatt tccgccatga tctcgagcgc ttcttcgccc agcccggcaa acgcgtaggg
     3601 cgagctgcgc ggcttgaaag cgccaccccg gaaaaaacgc gcgccacttt tttgcaccga
     3661 ctccgcaatc gcgaacgcct gcgcccgcga ttcgatcgca cacggcccgg caatgatcgc
     3721 caggccgcca tcaccgattt tcgcgtcgcc gatttcaatc acggtcccct ccggccggaa
     3781 ttcgcgagtg acgagtttgt agggtttgct gattgcgatt gcctcggcca cgcccggcat
     3841 gttttcgaaa tgcgcgggat cgaccgcgcc ggtgtttccg gtcaggccga tgaccgtccg
     3901 gatcgctcca ggcatggggt gggcgcgaaa gccgagttgc gtgatggccc gcacgacgcc
     3961 ggccagttcg gcctcggtcg cacgggcggt cattacaatc agcataaatt caggagtcga
     4021 tcacggggtg ctcctgcagt ctcgttcggc acttggacgt cgcaagctcg ctcgcgtttc
     4081 gcaacactgc tgggaccagc gcccgcctca ggcggactac aactcgttag gcgtcgtcgc
     4141 ccgtcccctc gcccttcgtc gccttctgcc cgcgcaactt ggcctggatg aaaagatcaa
     4201 tgccgccatc catcaccgcc tgcacgttgc ttgtctccgc gccggtgcgg tgatctttca
     4261 ccatctgata cggctgaaaa acatacgatc gaatctggct cccccatgcg acgtcgcctt
     4321 tctcgccata ttgccggtcg atctcagcgc gctttttgtc ctgctcgatc tcgtaaagtt
     4381 ttgccttgag cattttcatc gcgagctcac ggttgcggcc ctggctgcgc tccgcctggc
     4441 aggcgacgac gattccgctc ggcttgtgca cgatgcgcac cgcggtttcg actttgttca
     4501 cgttctgacc acccttgccg ccggcgcgga aggtgtcgat ttcgaggtct gcgggattca
     4561 cttcgatcgg cgcggagtcc gcgatttccg gcacgacatc aacgctcgcg aaggaggtgt
     4621 gccgccgctt gttcgcgtcg aagggcgaga tgcgcacgag acgatgcacc ccgcgctccg
     4681 ggttcaaata gccgtaggca tattcgccgc tcacgagcag cgtgaccgat ttgatcccga
     4741 cttcttcgcc ctgctgaagg tcgatcgttt gcgcgctgaa accgctccgc tcgatccagc
     4801 gctgatacat ccgcagcagc atgtcggccc aatcgcacga ctccgtcccc ccggcgccgg
     4861 agtgaatcgt gacgaacgcg ttcgagcgat cattttcgcc ggagagaaat tgtcggagct
     4921 caaatgcctc gagcttttgc acgaagcgcg catgctcggt cgcgatctct ttctccgccg
     4981 cggcgcgcgc cgccggatca gtttcctccg ccgccagttg ttgcagcgct tcgaaatctt
     5041 ccgcctcgcg ctcgagctcg agcagcgggt tgatcaaacc gcgcaggcgc gaaacctcct
     5101 cgacttcgcc ctgggcgcgc tcgcggcttg accagaaatc cgcggccgcc atccgcgttt
     5161 cgagttccgt caggcgggct tgcagcttcg gcaggtcaaa gaaacctccg cagttcgccg
     5221 acgcgcgcgc gcaattcttc tgcgttgatt gcttccactt ccgttgccat gactaaagat
     5281 aatcggacgc gctctcgtgc caaatcagat caacgggcgc catccgcgac ggcgcaaata
     5341 aacagacgcg ccttaagccc gaagcgacgg ttgtaggtct gcgccagctc atccgcgatc
     5401 cccgcggaag cgacgttttc gcaaagggtc accgtggcac cgccaaagcc gccgccggtc
     5461 aatcgcgcgc cataaacgcc tggaagatcg cgcgcgatct cgacgagcag atcgagttcg
     5521 gcggtgctgt tctcaaaatt ttgccgcgag ctttcgtgcg attcgttcat cagcgcaccg
     5581 cagccgcggg cgtcgttctc cgcgagcagg atgagggcgc gttgcacccg ttcgttttca
     5641 ccaacaatgt gcgccgcgcg ccggcggagg agtggcggca gatcgcgttt ggctaattcg
     5701 ttaggcgaaa tgtcgcgcaa cgcaaccaga ccgagcgccg ccgcggcggc ccgcgtttcc
     5761 tcgcatcgct ggtcgtaaag gccttccgcc agctcacgtt ttgtgcccga ctcggcgatg
     5821 accagggaga gctccggcgg aaatggaatc gtgcgcactt cctcgctccg cgcatcgaag
     5881 aagagggcat gctgcgcgcg gccgaaaatg gacgtgacct ggtcgagcaa gccggatttc
     5941 actccgacga accgatgctc ggc
//
LOCUS       DATMNS010000247         3295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3068552, whole genome shotgun sequence.
ACCESSION   DATMNS010000247 DATMNS010000000
VERSION     DATMNS010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3068552"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VNW28_08675"
     CDS             <1..763
                     /locus_tag="VNW28_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXA10042.1"
                     /translation="PRAKILAHPGMLYVAIGIIGATVMPHNLYLHSSIVQTRKFERTP
                     VGQREAIKFGTIDSSAALMFALFINAAILVLAASAFHWSGHQDVAEIQDAFKLLSPLL
                     GVGMASVLFAIALLASGQNSTLTGTLAGQIVMEGFLNFRIAPWLRRVITRGLAIVPAV
                     LVIGIFGEKETTQLLIASQVVLSMQLGFAVWPLMRFTGEKKKMGEFANGFWIKLFGWA
                     TALLIIALNVKLLFDTFLPAAWTGAFYGALGLPAP"
     gene            872..2335
                     /locus_tag="VNW28_08680"
     CDS             872..2335
                     /locus_tag="VNW28_08680"
                     /inference="COORDINATES: protein motif:HMM:NF018983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016977 - chitosanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 75 protein"
                     /protein_id="HXA10043.1"
                     /translation="MRKFRLSTLRRVTLLLFFALFGAIVLRWISLERLRLSLQRLGPP
                     LLPSPSVTPELIPTPTRPPVIAGKLDTAKLWSGITVHAQVDPTPGREASVERLDPQSY
                     VLDLRLNVRVPTPNRTLEELAQVTPELPKLLPHLGATLTPDPVSPLFQQLYDLKVQTL
                     REDLVRLDQLLSRHNFYDCQTILQLRDPATKRTGILLQADMDVDADGSDADRVPEIDG
                     RSANFKPFTSYRWKKKMARPNPFLPAAEEKLRRYETEFAQKGLPMERNRDLRIGIQRM
                     KAEVDSLKRFSFLIAATDPYIVLPGVFARAKDAGRVGDYAVVLFGDQIYPAIVGDVGP
                     SDKVGEASLRIAKEINVLATPYNRPVSDLKVTYLIFPETADSPFGPPDLEKIQARCEK
                     LVQEIGGASVPLHHWQNLIPTPTPTPSATPAPTPSPSATPSPNESATVSPSPVVAPTF
                     AFPTPPESPSPSGSPSPASPPSLLPTSSPLASLAPSP"
     gene            complement(2392..3102)
                     /locus_tag="VNW28_08685"
     CDS             complement(2392..3102)
                     /locus_tag="VNW28_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10044.1"
                     /translation="MTIFLTFFARTTISPAMSKLVVALLSVTFFLAGCAEAPQSQSTG
                     THSSAAAASVAAVRTQPAASKRSVWVEPPTGSHIGGGFARASGNTGSNDEPGLISAIN
                     SINQANAHQREQPFVLAAFSQVSGLSQQQVLTLQNQTRLPLGDLLAFNTIARNRQPKV
                     QELAALKASGKSWAALAGANAMNIATVAQTVRTANDLAIRSYLSASENSPGTVQQLQN
                     VGARTQTTGPGGSRPVGP"
BASE COUNT          601 a         1049 c         1001 g          644 t
ORIGIN      
        1 tccgcgcgcg aaaatcctgg ctcatcccgg catgctttat gtggcgatcg ggatcatcgg
       61 cgcgaccgtc atgccccata atctgtatct gcattcgtcg atcgtgcaga cgcgcaaatt
      121 cgagcgcacg ccggtgggcc agcgcgaggc gatcaagttc gggacgatcg attcgagcgc
      181 cgcgctcatg ttcgcccttt tcatcaacgc cgcgatcctc gtcctcgcgg cctcggcgtt
      241 ccattggtcg ggacaccagg acgtggcgga aatccaggat gctttcaagc tgctcagccc
      301 acttctcggg gtcggcatgg cgagcgtcct cttcgccatc gcgctcctcg cttccggtca
      361 aaactccact ctcaccggga cgctggccgg gcagatcgtg atggagggat tcctcaattt
      421 ccggatcgcg ccctggctgc ggcgggtgat tacgcgcgga ctggcgattg tcccggcggt
      481 gttggtcatc ggcatcttcg gggaaaagga gacgacccag ttgttgatcg cgagccaggt
      541 ggtgttgagc atgcaacttg gtttcgccgt ctggcccctg atgcgtttca ccggcgagaa
      601 gaagaagatg ggcgaattcg cgaacggctt ttggatcaag ctcttcggct gggcaaccgc
      661 gcttctcatc atagcgctga atgtaaaact gctcttcgac acctttctgc cggctgcgtg
      721 gaccggcgca ttttacggcg cgctcggtct gcccgcgccg tgatcgcctg cctaacgaaa
      781 ccgcccggct agacagcacg cgcgaactgg cttaacgagt ctcgcagcaa ccgctcatgt
      841 tgggttcaac cagaatttgc ttcctcttcg cgtgcgcaag ttccgcctaa gcacgctgcg
      901 ccgggtcacc ctgctgcttt ttttcgcact cttcggcgcg attgtcctgc gctggatttc
      961 gctcgagcgt ctgcgcctga gtctgcagcg tctcggtccg ccgctgctcc cgtcgccgag
     1021 cgttaccccg gagctcatcc cgacgccgac gcgaccgccg gttatcgccg ggaaactgga
     1081 cacggcaaag ttgtggagcg gcatcacggt ccacgcgcaa gtcgatccga cgccgggccg
     1141 cgaggcctcg gtagagcggc tcgatccgca gagttacgtg ctggatttaa gactgaatgt
     1201 gcgcgtgccg acgccgaaca gaacgctgga ggaactggcc caggtgacgc cggaactgcc
     1261 gaagctgctg ccgcacctcg gcgcgacgct gacaccggat cccgtctcgc cactttttca
     1321 gcaactctac gacttgaaag tccagacgtt gcgggaagat ctggtgcgac tggatcaact
     1381 gctttcccgt cacaacttct acgattgcca gaccatcctg cagttgcgcg atccggcgac
     1441 gaagcgcacc ggcattctgc tccaggccga catggacgtc gacgcggatg gttccgacgc
     1501 ggatcgcgtg ccggagatcg acggacgctc ggcgaatttc aagcccttca ccagctaccg
     1561 ctggaagaag aaaatggcgc gccctaatcc gttcctcccc gcggcggaag agaaattacg
     1621 ccgctacgaa accgagtttg cgcaaaaagg cctgccgatg gagcggaacc gcgacctgcg
     1681 gatcgggatc caaagaatga aggccgaggt cgactcgctc aagcgcttca gtttcctcat
     1741 cgcggcgacc gatccctaca tcgtgctccc gggagttttc gcgcgcgcga aggatgccgg
     1801 acgggtcggc gactacgcgg tcgttctctt cggcgatcag atttacccgg cgatcgtggg
     1861 tgacgtcggg ccgagcgaca aggtcggtga agcctcgctc cggatcgcga aggagatcaa
     1921 cgtgctggcg acgccctaca accgcccggt gagcgacctc aaagtaacct acctgatttt
     1981 tcccgagacg gctgactcgc cgtttgggcc acccgacctg gagaagattc aggcgcgttg
     2041 cgaaaagctc gtgcaggaaa tcggcggtgc gagcgtgccg ttgcatcact ggcagaatct
     2101 aatcccgacg cccacgccca ctccgtcagc cacgcctgcg ccgacgccgt caccgagcgc
     2161 gacaccgtcg cctaacgaat cggcgacagt gtcgccttcg ccggtcgttg ccccaacttt
     2221 cgctttcccg acgccgcccg aatcgccctc gccctcaggc agcccttcgc cggcatcccc
     2281 gccgtcgctg ctgccgacaa gttccccgct cgcctctctc gcgccgagcc cctaacgagg
     2341 agccacgcgg tggacgcgtt ttgaggcctg gtcgcgcggc agccgccgca actacggccc
     2401 gactgggcga gagccccccg ggccagtggt ttgcgtgcgc gctcccacat tctgcaattg
     2461 ctgtacagtc cccgggctat tttccgaggc gctcaaataa gaccggatcg cgagatcgtt
     2521 ggcggtgcgc acggtctggg cgacggtggc gatgttcatg gcgttggctc ccgcaagtgc
     2581 ggcccaggat ttgccacttg ccttgagagc ggcgagctct tgcaccttgg gctgacgatt
     2641 gcgggcgatg gtgttgaaag ccagcaggtc accgagcgga agacgcgtct gattctggag
     2701 agtcaatact tgttgctggc tcaggccgct gacctgggag aaagccgcga ggacaaatgg
     2761 ttgctcacgc tgatgagcat ttgcctggtt gatcgagttg atcgcactga tcagccccgg
     2821 ctcgtcattg ctccccgtat tgcccgaggc gcgcgcgaaa ccgcccccga tatggctgcc
     2881 tgttggcggc tcgacccaaa ccgaacgttt tgatgccgcc ggctgagtcc ggaccgccgc
     2941 aacgctggcg gcggcggcac tcgaatgcgt tcccgtggat tgagattgag gtgcttcggc
     3001 gcaaccggcc aggaagaaag taaccgagag aagagcaacg accagtttcg acatggcagg
     3061 cgagatagtc gtgcgcgcga aaaacgtcaa gaaaattgtc atcaaaacgt aacaattgag
     3121 cttttgcccg ggtccttttc cctcgttaga aagaggcgtg gtgatcaacc gcacgaatcg
     3181 ctatcgcgca gcgtggatct ccgacgtcca cctcggcact cgtgggagca acgctgcggc
     3241 gctcctcgcg ttcttgaagg agtacgagtt cgacacgctc tacatcgtgg gtgac
//
LOCUS       DATMNS010000248         4710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3433151, whole genome shotgun sequence.
ACCESSION   DATMNS010000248 DATMNS010000000
VERSION     DATMNS010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3433151"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..684
                     /locus_tag="VNW28_08690"
     CDS             196..684
                     /locus_tag="VNW28_08690"
                     /inference="COORDINATES: protein motif:HMM:NF016213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HXA10045.1"
                     /translation="MELFWWGVTLVLMAVGLIGTVLPIFPGTTVILAAAIMHRFMLGP
                     ARSIGGWSIALLVALTLVSYALDLVGGLYGARRFGASRWGVAGAVLGAITGLFFGLPG
                     LLVGPIIGAVAVEIICGKRLVAAGRAGWGTLLGNLAGMLGKLVIAFAMVSWFLVAVPA
                     PF"
     gene            complement(681..1244)
                     /locus_tag="VNW28_08695"
     CDS             complement(681..1244)
                     /locus_tag="VNW28_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10046.1"
                     /translation="MFRFHLFFSISCCVLALSSTRAAGLSKSETRFVYVDARGKVQST
                     SIISRFTGKTKNPFPHLDPSLARAATIAEERAHAHSKMCCWRYVKEALLASGAVDSYP
                     KSELARQAGDELVQRYGFKRLAVYDPYQAPIGAVLVYGSGRAAGHVEIRTAHGFASDF
                     RTPTPSRRPLIGVYAKTSSETKGNRFF"
     gene            complement(1567..2826)
                     /gene="fahA"
                     /locus_tag="VNW28_08700"
     CDS             complement(1567..2826)
                     /gene="fahA"
                     /locus_tag="VNW28_08700"
                     /EC_number="3.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004334 - fumarylacetoacetase activity
                     [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetase"
                     /protein_id="HXA10047.1"
                     /translation="MATSLRSFIEVAPGSHFSLQNLPYGIFRPGDDPARAGVAIGDLV
                     LDLAVLEEGGYFRALDFGPRAIFAGDSLNAFLALGRPAWRKTREVLQHLLAAETATLR
                     DDHALRARAFHQQKEITMQLPARIGDYTDFYSSYHHAFNVGTMFRGPENALMPNWKWL
                     PIAYHGRASSVVPSGTEVRRPHGQIKPPDAEAPIFSASRALDFELETAFFVGPPNELG
                     QPVPIAEATDHIFGLVLMNDWSARDIQTWEYQPLGPFLAKNFCTSISPWVVTLEALEP
                     FRKPLPAQDPPPLPYLRAPNDFTFDIRLEAQLQTAKMSAPQTITRSNFQNLYWSMAQQ
                     LAHHTVGGCNLRPGDLLASGTISGPAEDGRGSMLELTWRGANPLQLPGGETRKWLEDG
                     DRLTISGWCEGDGYRVGFGEVTGRIVG"
     gene            complement(2829..3737)
                     /locus_tag="VNW28_08705"
     CDS             complement(2829..3737)
                     /locus_tag="VNW28_08705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10048.1"
                     /translation="MSSASLRALLGHAIDYAGLFPPADLALEPALRDQAGYVRSPDRW
                     MLGAFILPVAKFAAARAQLKQFEREHPLQISALGPKTAKAADFRRALEKAAKAIKEFS
                     TKHEGVVSIAQFEMPLPPGPVADSLGVARATLGDLALNVFWEAPAAAAASTIEALASS
                     GAGFKLRTGGVSADAFPTDGQIARALVAAVRHRVPIKFTAGLHHPVRLFHQSVGTKMH
                     GFLNVLGAGVLAAEHRWSEGQTQEMLADESPGSFAFEEEAFRWRDWKITTAQITARRR
                     LVTSLGSCSFDEPRDDLRALGLLERS"
     gene            3850..>4710
                     /locus_tag="VNW28_08710"
     CDS             3850..>4710
                     /locus_tag="VNW28_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXA10049.1"
                     /translation="MEIELEKEVSRSDDPGDPTGNTVVTPRMREHDPVPRPFDVRSVA
                     LTGLFLLAIFYTIYFLRALLLPLVLALLLSYLLRPIVRGLVRIKIHPVIGSALLLLSL
                     VGAITYSVSLLAAPAAGWLEKAPYSLRQLQDRLTPFKKPMAKVAQASGAIENLTATES
                     VAARTPTVELKQHPIAEGLYAHTSEFLVSALTMLILLYFLLAYDGVFLAKLIKLMPTL
                     SDKKRAVSIAHEIEAQISRYLFTVTIINIGLGTAVGVVVGLLGLPNPSLWGVLVAVLN
                     FIPYLGALTGI"
BASE COUNT          882 a         1452 c         1469 g          907 t
ORIGIN      
        1 actttggcga gcgcgtttgg cggcagattt ggagttggcc atggatcaga attttgtgga
       61 cgggcaggct cgcggacgac cgaaacgttg tcaaggcggg cgcagctcgc agccgggagc
      121 ggtgacaccg gttggccggc acggcatcga gcgacttgat ggccgggatt accaactccg
      181 gttacagtcc gcgcgatgga actcttctgg tggggcgtga cgctggtcct gatggccgtc
      241 ggcctgatcg gcacggtgtt gccgatattt ccaggaacaa ccgtcatcct cgccgcggcg
      301 atcatgcacc gcttcatgct cgggccagcg agaagcattg gcgggtggag catcgccctg
      361 ttggttgcgc tgaccctcgt ctcctacgcg ctcgacctag tcggcgggct ttacggcgcg
      421 cggcgtttcg gcgcgagccg ttggggcgtt gccggtgcgg ttctcggggc gatcactggc
      481 ctgttcttcg gcctgcccgg actccttgtt gggccgatca tcggcgccgt cgcggtcgaa
      541 ataatctgcg gcaaacgcct cgttgccgcg ggccgcgccg gctggggtac gttgctaggc
      601 aatctcgccg ggatgctggg caaactcgtc atcgcgtttg cgatggtgag ctggttcctg
      661 gtcgccgtgc cggcaccgtt ttagaaaaat cggttcccct tcgtttctga ggaggttttc
      721 gcgtagaccc cgatcaacgg ccggcgcgag ggcgtcggtg tccggaaatc gctcgcgaat
      781 ccgtgcgcgg tgcgaatctc aacatgcccg gcggcacggc cggatccata caccagcacg
      841 gcgccgattg gagcctgata ggggtcatag acagcgaggc gcttaaatcc gtagcgctgc
      901 accagttcgt cgccagcctg tcgcgcgagt tccgatttcg gataggaatc gaccgcgcca
      961 gaagcaagca atgcttcttt cacgtaacgc cagcaacaca ttttcgaatg cgcgtgagcc
     1021 cgctcctcag cgatcgtcgc ggcgcgggcc aggctcggat cgaggtgcgg aaagggattc
     1081 ttcgtcttgc cggtaaaccg cgagatgatg gacgtcgact gcactttgcc ccgggcatcg
     1141 acgtagacaa aacgcgtctc cgatttcgag agaccggcgg ctcgtgtgct cgagagagcg
     1201 aggacgcagc agctaatgga aaagaaaagg tgaaaacgaa acacgcgaag gagctttatg
     1261 caggcaacaa tggtgccgcg caaccataaa tttttcgcaa ggcgcattta atttttggtg
     1321 gcgcgatcag ttccgcggga aaaacccggg ggtgaggcgg cttttcgcgc tcggccgcat
     1381 tctggaacga ttacgccgct ctcggctcct agaaaacctg ttgaaacgtt acctggagca
     1441 tggccaccga cagcgaaaat taagaagcgc ccggaccaaa tcggaggaac tgctaggaaa
     1501 gcccgtttgc aggatggcct ttctttttgt ccgagatctc acctttgggg gcggggcaca
     1561 ggtgagtcaa ccaacaatgc ggccggtcac ttcgccgaag ccgacccggt agccgtcgcc
     1621 ttcgcaccac ccgctgatcg ttaggcgatc gccgtcctcg agccatttgc gcgtctcgcc
     1681 gcccggcaat tgcaacgggt tggctccgcg ccaggtcagt tcgagcatgc tgccgcgtcc
     1741 gtcttccgcc gggccactga tcgtcccact ggccagcaaa tcaccgggcc ggaggttgca
     1801 tccgcccacg gtgtgatgcg cgagctgttg cgccatgctc cagtagaggt tctggaaatt
     1861 gctccgcgtg atcgtttgcg gcgcgctcat cttcgcagtc tgcaactgcg cttccaaccg
     1921 aatatcgaag gtaaagtcgt taggcgcgcg caggtaaggg agcggcggag gatcctgcgc
     1981 cggcagcggt ttgcgaaaag gctcgagcgc ttccagcgtt accacccagg gcgaaatgct
     2041 ggtgcagaaa ttcttcgcca agaacgggcc gagcggttgg tattcccacg tctggatgtc
     2101 gcgcgcactc cagtcattca tcaggacgag cccgaaaata tgatcggtcg cctccgcgat
     2161 tggcaccggc tgaccgagct cgttcggcgg tccgacgaag aaagcggtct cgagctcgaa
     2221 gtcgagagcg cggctcgcgc tgaaaatcgg cgcctcagcg tcgggcggct tgatctgacc
     2281 gtgcggccgg cgcacctcgg tcccgctcgg gacaacggaa ctggcgcggc cgtggtaggc
     2341 gatcggcagc catttccaat tcggcatgag cgcgttctcc ggcccgcgga acatggtgcc
     2401 gacgttgaag gcgtggtgat aggaagaata gaaatcggtg taatcgccga tccgcgccgg
     2461 caattgcatc gtgatttctt tttgttgatg aaatgcccgg gcgcgcaagg cgtggtcatc
     2521 gcgcagggtg gcagtttcgg ctgcgaggag atgttgcaac acctcgcgcg ttttgcgcca
     2581 ggccggacga ccgagcgcca aaaacgcatt cagcgaatca ccggcaaaga tggcgcgcgg
     2641 gccaaaatcc agcgcgcgaa aatagccgcc ctcctcgagc accgcgaggt cgagcaccaa
     2701 gtcgccgatc gccacgccgg cgcgcgccgg gtcgtcgcca ggccgaaaga ttccgtaggg
     2761 cagattctgg agcgaaaaat gcgatccggg cgcgacctcg atgaaggacc ggagtgatgt
     2821 ggccatggtc aggatctttc caaaagtccg agcgcccgca ggtcgtcgcg cggctcatcg
     2881 aagctgcaac tgcctaacga cgtcaccagg cggcgacgcg ccgtgatttg cgccgtcgtg
     2941 attttccaat cgcgccagcg gaaggcctcc tcctcgaagg caaacgagcc tgggctttcg
     3001 tctgccagca tttcctgcgt ctgcccctcg ctccagcgat gctcggccgc gagcacgccg
     3061 gcgccgagga cgttgaggaa accgtgcatt ttcgtgccga cactttgatg aaaaagtcgc
     3121 accggatgat gcaacccggc ggtaaacttg atcggcacgc gatgccgcac cgcggcaacc
     3181 agagcgcggg cgatttgccc gtcggtcggg aaggcgtccg cgctcacgcc gccggtgcgg
     3241 agcttgaaac ccgcgccgct gctggcaagc gcctcgatgg tcgaggcggc ggcagcggcg
     3301 ggcgcttccc aaaagacgtt caacgccagg tcgcccaggg tcgcgcgcgc cacgcctaac
     3361 gaatccgcga ctggccccgg tggcaacggc atttcaaact gcgcgatcga gaccaccccc
     3421 tcgtgctttg tcgaaaattc cttgatcgct ttcgccgcct tctccagcgc gcggcggaag
     3481 tccgccgctt tcgccgtttt cggtccgagg gcggagattt ggagcggatg ctctcgctcg
     3541 aattgcttca gttgcgcgcg ggccgcggca aatttcgcaa ccggcaaaat gaaagcgccg
     3601 agcatccagc gatccggcga acgcacatag cccgcctggt cgcgcagcgc cggctcgagc
     3661 gcgagatcag cgggcgggaa gagccccgca taatcaatcg cgtggccgag cagcgctcgt
     3721 aaagaagcag acgacatcgg gagaactcaa cggcgcaatc gtcgcgttcg tcaaccaatg
     3781 gaattctttt tggaaccgaa tctgacactt tgaagcgtaa gtccgtcgcg gcgcacgatt
     3841 catcggacag tggaaatcga gctcgaaaaa gaagtctccc gttccgacga ccccggagat
     3901 cccaccggga acacggtcgt cacaccgcgg atgcgagagc acgaccccgt gccgcggccg
     3961 tttgacgtgc gctcggtcgc actgaccggg ctctttctgc ttgcgatctt ttacacgatc
     4021 tacttcctgc gggcgctcct tcttccgctc gtcctcgccc ttctcctgag ctacctcctc
     4081 cgaccaatcg tgcgcggtct ggtgaggata aaaatccatc ccgtgatcgg cagtgccctg
     4141 ctcctcctct cgcttgtcgg cgcgattacc tacagtgttt cacttctggc ggcgcccgcg
     4201 gccggctggc tcgaaaaggc gccttacagt ttgcgacagt tgcaagatcg tctcacgcct
     4261 ttcaaaaagc cgatggccaa ggtcgcgcag gcgagcggcg cgatcgagaa cctgacggcc
     4321 accgaatccg tcgccgccag gacgccgacg gtcgaactca aacagcaccc aattgcggaa
     4381 gggctttacg cgcacacctc ggagttcctg gtgagcgcct taacgatgct catcctgctt
     4441 tacttcctgc tcgcctacga tggcgttttc ctcgccaagc tgatcaagct catgccgacg
     4501 ctttcggaca agaaacgggc ggtctccatc gcgcacgaaa tcgaagccca gatctcgcgt
     4561 tatcttttta ccgttacgat catcaatatc ggtctcggga ccgcggttgg ggtagtagtg
     4621 ggattactgg gcctgcccaa cccgtcgctc tggggcgtgc ttgtcgcggt gctcaatttc
     4681 atcccctatc tcggcgcgct caccgggatt
//
LOCUS       DATMNS010000249         2750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3439783, whole genome shotgun sequence.
ACCESSION   DATMNS010000249 DATMNS010000000
VERSION     DATMNS010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3439783"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VNW28_08715"
     CDS             complement(<1..217)
                     /locus_tag="VNW28_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10050.1"
                     /translation="MKSKSAAEAGRKPSSLAPGRTLTRNGKGKRRGGCRPLEKDSPNP
                     PDDRYRTLFDANPTPMWIYDLETLRFLE"
     gene            398..1933
                     /locus_tag="VNW28_08720"
     CDS             398..1933
                     /locus_tag="VNW28_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HXA10051.1"
                     /translation="MLSRIYSAAVYGVDAYEVEIEVNAAGGSPNIVIVGLPDAAVKES
                     RDRVTTAISNSGYYWPRGRTTINLAPADIKKEGPSFDLPIALGMIAAAQEIEDELFDQ
                     FSFVGELALNGAVRAVKGVLPVALEARRRGKRGVIVPEANAAEAAMVEGIDVYGMQNL
                     RQTFQFVRGETPLQPVRGDLSQFFATHQTYDVDFADVKGQGHVKRAVEVAVAGGHNIL
                     MIGPPGSGKSMLSKRIATIIPPMALEEAIEATKIHSIAGLLTPEQSFVATRPFRSPHH
                     TISDVGLLGGSAIPAPGEVSLAHHGVLFLDELPEFKRSTLEVLRQPLEDGRVTISRAA
                     GTITFPSEFMLVAAMNPCPCGYFGDLKRECRCSPVQVQRYRQRISGPLLDRIDLHIEV
                     PAVEYRDISSERAEEHSMAIRARVGLARERQQTRFHSDKKTTCNARMTSRAIKVHCKL
                     SDDSQELIRVAMTELNLSARAYDRILKVSRTIADLEGAAAISPEHVSEAIQYRTFDRT
                     LWV"
     gene            2088..2399
                     /locus_tag="VNW28_08725"
     CDS             2088..2399
                     /locus_tag="VNW28_08725"
                     /inference="COORDINATES: protein motif:HMM:NF025577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4326 domain-containing protein"
                     /protein_id="HXA10052.1"
                     /translation="MKSKQTKPHRVVRRRTRNWKMPADTVYVGRPTVWGNPFVVGSEL
                     IGGEKLTAAKAVALYRQYARDAFNLRDLRACLRGKDLACWCPLGQPCHADVLLELANL
                     A"
     gene            2538..>2750
                     /locus_tag="VNW28_08730"
     CDS             2538..>2750
                     /locus_tag="VNW28_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10053.1"
                     /translation="MFKTIHSFFPAQVRHLALFCAPLLLLSASAADESSSPSTSATSM
                     TENPLLKESALDFHYPPFDRIKDADFA"
BASE COUNT          515 a          866 c          803 g          566 t
ORIGIN      
        1 cttcgagaaa gcgcaacgtc tcgaggtcgt aaatccacat cggcgtcggg ttcgcgtcga
       61 agagggtgcg atacctgtcg tccggaggat tcggcgagtc cttttccagg ggccggcagc
      121 ctcctcgcct tttgcccttt ccgtttcggg tgagggttcg gcctggcgcc aggctgctcg
      181 gtttgcgacc ggcctccgca gctgatttgg atttcatctc gattcgatcg ggcggcgtta
      241 acctgggatt ttttacgtcg agggtgtcga aagcaggatt tcggccaacc ggtgtgccgc
      301 aagccttcgt cgacgttggc cgcgaccgag atcgccccca gtattttttt caaccgcagt
      361 ttggacttcg tccgcgtctt ctgacactgt cgcacccgtg ctctccagga tttattccgc
      421 agctgtttac ggcgtggacg cttacgaggt cgaaatcgag gtcaacgccg ccggcggcag
      481 ccccaatatc gtcatcgtcg gcctgccgga tgcggccgta aaggagagcc gtgaccgggt
      541 cacgaccgcg attagcaaca gtggctacta ttggccgcgc ggccggacga cgatcaatct
      601 cgcgccggct gacatcaaga aggaaggacc gagtttcgat ctgccgatcg ctctcggcat
      661 gatcgcggcg gcccaggaga tcgaggacga acttttcgac cagttcagtt tcgtcggcga
      721 gctggcgctc aatggcgcgg tccgcgcggt gaagggcgtg ctgccggtgg cgctcgaagc
      781 gcgccggcgc ggaaagcgcg gcgtgattgt gcccgaagcg aatgcggccg aagccgccat
      841 ggtcgaaggg atcgatgtct atggcatgca gaacctgcgc cagacttttc agttcgttag
      901 gggcgaaact ccactgcaac ctgtgcgtgg cgatctcagc caatttttcg ccacccacca
      961 gacgtacgac gttgattttg ccgacgtcaa aggccagggc cacgtgaaac gtgcagtcga
     1021 ggtcgcggtc gcgggcggac acaatatttt gatgatcggg ccgcccggct ccggcaagtc
     1081 gatgctttcc aaacgcatcg ccaccatcat cccgccgatg gcgttggagg aagcgatcga
     1141 ggcgacaaaa attcacagca tcgcgggtct gctcacgccc gagcagtcgt ttgtcgcgac
     1201 gcgtcctttt cgctcgcccc atcacaccat ctcggatgtg gggttgttag gcggctcggc
     1261 cattcccgcg ccgggcgaag tcagcctggc gcaccatggc gtcctctttc tcgatgaatt
     1321 gccggagttc aagcgttcca ccctcgaggt gttgcgccag ccgctcgaag atggccgcgt
     1381 caccatctcg cgcgcggccg gcacgatcac ttttccctct gagttcatgc tcgtcgccgc
     1441 gatgaaccca tgcccgtgcg gttatttcgg cgatctcaag cgcgaatgcc gttgcagtcc
     1501 ggtgcaggtg caacgttacc gccagcgcat ttccggaccg ctgctcgacc ggatcgatct
     1561 gcatatcgaa gtgccggcgg tggaataccg cgatatctcg agcgagcgcg cggaggagca
     1621 ttccatggcg attcgcgcgc gcgtcggcct ggcccgcgaa cggcaacaga cccgcttcca
     1681 ttccgacaag aagaccacct gcaacgcgcg gatgacatca cgcgcgatca aagtgcactg
     1741 caaactaagc gacgactccc aggaattgat ccgggttgcg atgaccgagc tgaacctgag
     1801 cgcgcgcgct tacgatcgaa tcctaaaagt ttctcgcacc atcgccgatc tcgagggcgc
     1861 cgccgcgatc tcgccggaac acgtgagcga agcgatccag tatcgcacct tcgaccgcac
     1921 cctttgggtt taacaaaggg ccaggggcga attgcagacg cctgcctgtg ggaggcgcgg
     1981 gtctcaggcc gtgccgcgcg atgagtcata tgcgaacgaa actctcgcgc ttcgcaaagc
     2041 gaagccgaca agccttgcga ccgcgtttgc agcggcgagt ttcctgcgtg aagtcgaaac
     2101 agaccaaacc gcatcgcgtg gtccgtcggc ggaccaggaa ttggaaaatg ccggccgaca
     2161 cggtctacgt cggccgtccc accgtctggg gcaatccatt tgtcgtcggc tccgagttga
     2221 ttggcggcga aaagctgacg gcggccaagg cggtcgcgct ctatcgtcag tatgcgcgcg
     2281 acgccttcaa cctccgcgat ttgcgcgcct gtttgcgcgg gaaagacctg gcctgctggt
     2341 gtccgctcgg ccagccctgc catgctgatg ttctgctcga gctcgcgaat ttggcctaac
     2401 gcattccctg gaccgattgc ctccgaacct accgatggct tgatctgtga aacacctttc
     2461 tcgatccaaa acccgatgta aatagtcagg ttcgatacct cagcaaccca ccctgcaatc
     2521 caacttccta acgaaatatg ttcaaaacca tccactcttt ttttcccgcg caggtccggc
     2581 atctcgccct cttctgcgcg ccgctccttc tcctttccgc gtccgccgcg gatgagtctt
     2641 cgtcaccttc cacaagtgcc acatccatga ccgaaaaccc gttgctcaaa gaaagtgcgc
     2701 tcgattttca ttacccgccc ttcgaccgga tcaaggatgc agatttcgcg
//
LOCUS       DATMNS010000250         4360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2013038, whole genome shotgun sequence.
ACCESSION   DATMNS010000250 DATMNS010000000
VERSION     DATMNS010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4360
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2013038"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VNW28_08735"
     CDS             complement(<1..1578)
                     /locus_tag="VNW28_08735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HXA10054.1"
                     /translation="MQKELTVRGLIIGVFITLVFTAANVYFGLKAGLTFSTSIPAAVI
                     SMAILRAFKDATVQENNIVQTVASAAGTLSSIIFVLPGLIMIGWWTGFPFWISFLICA
                     LGGILGVMYSIPLRRALVTNSDLPYPEGVACAEVLKVGSGGDKEHAADIEHGRAGLLA
                     VLWGSIVSAVFAVVVATQVFAADVAQTFRIGRRGAASGYDFSLSFALLAIGHLCGLSV
                     GIAMLIGAGIGWGWLVPHYSALTGDLTTAAGALAQSTWSHKVRFIGAGTIGVSAIWTL
                     LKLVKPVASGLAGAMAASRARKAGQADTLPITERDIPIGIVGVITLVCMLPIGWVLGY
                     FANTSGLGSHVTTLVIGGVFYVFLMSFFVSAVCGYMAGLIGSSNSPLSGIGILVVIGA
                     ALLLVVGVKPYVGPEATKALMAFALFTTAVIFNVAAIANNNLQDLKTGQLVDATPWKQ
                     QVALIIGVLAGAVVIPPVLNLVNQAYGFVGAPGAELRLHPLPAPQAGLISSLAQGVIA
                     ADIDWSLIRMGAYIGVAI"
     gene            complement(1639..1806)
                     /locus_tag="VNW28_08740"
     CDS             complement(1639..1806)
                     /locus_tag="VNW28_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10055.1"
                     /translation="MMESPFRETDGTAPVPPNRAAGLTNRDMDAGDDFLPRIERVTLG
                     EAIQLRRRYLS"
     gene            complement(1945..4104)
                     /gene="recQ"
                     /locus_tag="VNW28_08745"
     CDS             complement(1945..4104)
                     /gene="recQ"
                     /locus_tag="VNW28_08745"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HXA10056.1"
                     /translation="MELRAALKKHFGYSAFRPLQEEIVRDALAGRDVFALMPTGGGKS
                     LCFQLPALLREGLTIVISPLISLMKDQVDALQASGVAATFLNSALDATQARSRLRGLY
                     NGEFHLLYVAPERLMLESFIEKVKEWNVAQIAIDEAHCISEWGHDFRPEYRELVRLRE
                     LLPKVPIMALTATATERVRGDILEQLKLREPRCYVASFDRPNLTYRVVPKSAPYDQLL
                     EFLRTRPNDSGIVYCASRKTADSVAAKLNADGIAAKPYHAGLEAKERAQNQEMFLRDD
                     VRVVSATIAFGMGINKPNVRFVVHYDLPKNIESYYQETGRAGRDGLPSDCVLLFSASD
                     VVKQTRFIEEKSESEARIAREQLRQMVHYAETRECRRTTLLRYFAEERPHQSCNGCDN
                     CLNPRETFDGTVAAQKFLSCVLRVHQKSGFAFGLNHIVEVLTGADTEPIRQRGHNKIS
                     TYGIGSELKRDAWQALGRELLRLGLVEVAPGKFATLQVTEAGRAALRGRTPITLTKQV
                     EKPTAKVKQRAGEIECDETLFEKLRGVRRVLADARNVPAYVIFSDVALREMARSYPTT
                     PIEFRRIPGVGEQKLKDFAEPFLAAIADYLQSNPRQAFAAPNELRSRAAARLNDSEAE
                     TLRRFQAGESIDQIARRRGFVDRTIQDHLVKAIEAGAPLALEQFFTSAQAQELASAMS
                     QLGPRGLTGLGEELGGKYELAELRLFRALAARERRSA"
     gene            complement(4152..>4360)
                     /locus_tag="VNW28_08750"
     CDS             complement(4152..>4360)
                     /locus_tag="VNW28_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA10057.1"
                     /translation="FMFPFRGMPPWAQAIGEFFPLTHFLRIVRGILLKGNAFNDVLLQ
                     LWQIALFALIALIIGVKRYRQTLD"
BASE COUNT          854 a         1356 c         1374 g          776 t
ORIGIN      
        1 gatggcgacg ccgatatagg cgcccatccg gatcaggctc caatcgatat ccgcggcgat
       61 cacgccttgg gcgagcgacg agatcaggcc ggcctgaggt gctggcaatg gatgcagccg
      121 caattctgcg cccggcgcgc cgacaaaacc gtaggcctga ttcaccagat taagcaccgg
      181 cggaatgact actgcgcccg cgagaacacc gatgatgagc gcgacctgct gtttccacgg
      241 agtcgcatcg accagctgac ccgtcttcaa gtcttgcagg ttgttgttcg cgatcgcggc
      301 tacgttgaag atgacggcgg tggtgaaaag cgcgaaagcc atcagcgcct tggtcgcttc
      361 cggccccaca taaggtttca cgcccacgac aagcaacagg gcggcgccga tcacgaccag
      421 aatgccgatg ccggagagcg ggctgttcga tgaaccgatc aggccggcca tgtaaccgca
      481 gaccgccgaa acgaagaagc tcatcaagaa gacatagaag acgccgccga tgacgagcgt
      541 tgtgacgtgc gagccgagcc cgctcgtgtt tgcgaagtag ccgagcaccc agccgatcgg
      601 gagcatgcaa acgagtgtga tgacgccgac gatgccgatc ggaatgtcgc gttcggtgat
      661 cggcagcgtg tccgcttgtc ccgctttccg cgcgcgcgac gcggccatcg cgccggccag
      721 gccgctcgcg acaggcttca ccaacttcag cagcgtccag attgcggaaa cgccaatcgt
      781 cccggcgccg atgaatcgca ccttgtgact ccaggtgctt tgcgccagtg cgcccgccgc
      841 agtcgtcagg tcgccggtga gtgccgagta gtgcgggacg agccaacccc agccgatgcc
      901 ggcgccgatg agcatggcaa tgccgacgga caatccacac aggtgaccga tcgcgagaag
      961 cgcgaaggaa agagaaaaat cataaccgct ggccgcgccg cgccggccga tccggaacgt
     1021 ctgcgccaca tcagcggcaa atacttgcgt cgcgacgacg accgcaaaca ccgcagaaac
     1081 gatcgatccc cacaacaccg cgagcaaccc cgcgcgtccg tgttcgatat ccgcggcgtg
     1141 ctccttatcg ccgccgctgc cgaccttgag cacttcggcg caggcgacgc cttccgggta
     1201 aggcaggtcg gagttcgtga ccaacgcgcg gcgcagcgga atcgaataca tcacacccag
     1261 aatcccgccg agcgcgcaga tcaggaaaga aatccagaag ggaaacccgg tccaccagcc
     1321 gatcatgatc aaacccggca agacaaagat gatcgaagac aaagtgcccg cggccgaggc
     1381 aacggtctgc acgatattgt tctcctgcac cgtcgcatct ttgaaggcgc gcaggatcgc
     1441 catcgaaatc accgccgccg gaatcgaggt cgaaaaagtc aggccggcct tgagaccgaa
     1501 gtaaacgttg gccgcggtga agaccaacgt gataaagact ccgatgatca gcccgcggac
     1561 ggtaagttcc ttttgcatgt tggttgtgat gttctccgag cgacgcgagc ggaggatagg
     1621 gtggaatctc cgcgagggtc aagacaagta gcgccggcgg agttgaatcg cctctccaag
     1681 tgtgacgcgc tcgatcctgg gaagaaagtc gtccccggcg tccatgtcgc gattcgttag
     1741 gccggcggcg cggtttggag ggacgggcgc cgtcccgtcc gtctctcgaa acggggactc
     1801 catcatcgcg ccattgtttg gcgtgagcga tctcaggctt cgcaggagtt agcgtttact
     1861 tgaaagcccg tggcgacgag tcgcaggccc tggccgccaa acgaacgcaa caatcgctgg
     1921 cggtttttca ccgccctcca agggctaagc cgagcgccgc tctcgagcgg ccagtgcgcg
     1981 aaagaggcgc aactcggcaa gctcgtactt tccgccgagt tcctcgccca accccgtgag
     2041 gccgcgcggg ccgagctgcg acattgcgct cgccagttcc tgcgcctgcg cggaggtaaa
     2101 gaattgctcc agcgcgagag gcgcgccggc ttcgatcgct ttcacgagat gatcctgaat
     2161 cgtgcggtcc acaaatcccc gccggcgcgc aatctggtcg atcgattcgc cggcctggaa
     2221 gcggcggagc gtttccgctt cgctgtcgtt caaccgcgcc gctgcgcgcg aacgtaattc
     2281 gttaggcgcg gcgaatgctt gccgcggatt cgattgcaga tagtcggcga tcgcggccag
     2341 gaaaggttcg gcaaaatcct tcagcttctg ttcgcccacg cccgggatgc gacggaactc
     2401 gatcggcgtc gtcgggtaag agcgcgccat ttcgcgcagg gccacgtcgg agaaaatcac
     2461 gtaggccggc acgttgcgtg cgtcggccag cacgcggcga acgccgcgca gtttttcgaa
     2521 gagcgtttcg tcgcactcga tttcaccggc acgttgcttc actttcgcgg tgggtttctc
     2581 gacctgtttc gtgagagtga tcggggtgcg gccgcgcagc gccgcgcggc ccgcctcggt
     2641 cacctgcagg gtcgcaaact tcccgggcgc gacctcgacg aggcccaggc gcaggagttc
     2701 gcgtccgagt gcctgccagg cgtcgcgttt cagttcgctc ccgatgccgt aggtcgaaat
     2761 cttgttgtgg ccgcgctggc ggatcggttc cgtgtcggcg ccagtcagca cctcgacgat
     2821 gtggttgagg ccgaaggcga atccgctttt ctggtgcacg cgcaggacgc aggagagaaa
     2881 tttttgtgcg gcgaccgtgc cgtcgaaggt ctcccgtggg ttgaggcagt tgtcgcagcc
     2941 gttgcaggat tggtgagggc gttcctccgc gaaatagcgc aggagggtgg tgcggcggca
     3001 ttcgcgggtc tcggcgtagt gcaccatctg gcgcaactgc tcacgcgcga tccgcgcttc
     3061 gctctcgctt ttctcctcga tgaaacgggt ttgcttgacc acgtcgctcg cgctgaaaag
     3121 gaggacgcaa tcgctcggta atccatcgcg cccggcacgt ccggtctcct ggtagtagct
     3181 ctcgatgttt ttcggcaggt cgtagtgcac aacgaagcgg acgttcggtt tgttgatccc
     3241 catcccgaag gcgatcgtcg cgctcaccac ccgcacgtcg tcgcgcagga acatctcctg
     3301 gttctgcgcg cgctcttttg cttccaagcc ggcgtgatac ggcttcgccg cgatgccgtc
     3361 ggcattcagc ttcgcggcca cggaatcggc cgtcttgcga ctggcgcaat aaacaatccc
     3421 gctgtcgtta ggccgcgtgc gaaggaactc gagcaactga tcatagggcg cagacttcgg
     3481 cacgacccgg taagtcaggt tgggccgatc gaaactggcg acgtaacacc gcggctcgcg
     3541 cagcttgagc tgctccagga tgtcgccgcg cacgcgctcc gtcgcggttg cggtcagggc
     3601 catgatcggg accttgggca agagctcccg cagcctgacc agttcgcgat attccgggcg
     3661 gaaatcgtgg ccccattcgc tgatgcaatg cgcttcgtcg atcgcgatct gcgcgacgtt
     3721 ccactctttc accttctcga taaacgactc gagcatgagc cgctccggcg ccacgtagag
     3781 gaggtggaac tcgccgttgt agagtccgcg cagccgcgag cgcgcctgcg tggcatcgag
     3841 cgcggaattc aaaaacgtcg ccgcgacccc gctcgcctgg agcgcatcga cctggtcttt
     3901 catcagcgag atgagcggcg aaatcacgat cgtcaggccc tcgcgcaaca gcgccggcaa
     3961 ttgaaaacat agcgacttcc cgccgcccgt cggcatgagg gcaaagacat cgcgtcccgc
     4021 cagcgcgtcg cgcacgatct cctcctgcaa cggacggaag gccgagtagc cgaagtgttt
     4081 cttgagagcg gcgcgtagtt ccacagtccg gcagttttta cttccgcagc cgccggggca
     4141 agaacggagc gctaatcgag cgtctgccga tagcgtttga ctccgatgat gagggcgatg
     4201 agcgcgaaaa gcgcaatctg ccagagttgc aggaggacgt cgttgaaggc gtttcctttc
     4261 aataagatcc cgcgcacgat gcgcagaaaa tgggtgagcg ggaaaaattc gccgattgcc
     4321 tgggcccacg gcggcatgcc gcgaaatggg aacatgaatc
//
LOCUS       DATMNS010000251        10569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4133258, whole genome shotgun sequence.
ACCESSION   DATMNS010000251 DATMNS010000000
VERSION     DATMNS010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10569
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4133258"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VNW28_08755"
     CDS             <1..660
                     /locus_tag="VNW28_08755"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HXA10058.1"
                     /translation="TLSVKSRVGEPSEVGWLIAGAIIEALADRDPAINGVFHGLFDDQ
                     HAFEKGPKTVGDIWEILPYENFLVTGELLPIELKVVMEEVFESRETRSLAGFEMRTEG
                     KGSERRLTSLCLAERQPLDPAKRYRIALNTFDARSAGHRFMKLRSLLERSAAKCAFHP
                     VQTRDALIDYFRRHKVVYRHPRERPPAPLVIPGAVERSWGTGFEVTSAGSLDSAALRS
                     G"
     gene            708..1286
                     /locus_tag="VNW28_08760"
     CDS             708..1286
                     /locus_tag="VNW28_08760"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXA10059.1"
                     /translation="MLPDAFFKTDPLQCAKNLIGTQLIWEKCAGLVVETEAYLVKNDE
                     ASHTFRRPSTRAFIERNKPGAAYIYFNYGVHWMLNVLVKGGPRDGLILIRALEPQHGL
                     AIMRKRRGVEDVRELCSGPGKLTQALAISQRHHEIDLSSDPAHCFGPRPDPHVLVVAD
                     PRIGISRARDFPWRFTLRDSPFVSRPVQKAAR"
     gene            1321..2460
                     /gene="mnmA"
                     /locus_tag="VNW28_08765"
     CDS             1321..2460
                     /gene="mnmA"
                     /locus_tag="VNW28_08765"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXA10060.1"
                     /translation="MNGKKERVLLGMSGGVDSSVAGYLLREQGYDVVGVTMKVWPQDC
                     LSRAEDKCCGPQAIADARGVAHSLGIPYYVVDEADQFERVVIDYFSSEYQAGRTPNPC
                     VMCNEKLKFGSLWEKARALDCAYIATGHYAIIEHPAGVTDPGDNGADWPSRAVLRKGA
                     DPRKDQSYFLFSLRQAQLRHALTPLGKMAKPEIRAIARSLGLRVAEKVDSQEICFVPG
                     NDYRAFLRSHLGDTEFQRGGIYDLSGNFLAEHEGIELFTIGQRKGLPGGSPRPRYVVD
                     IDAMSNRVIVGEAEDLLVEEFEIDRLNWSSRAPSDEPRAVTVKIRYSHPGAAATLIPL
                     AGDRALVRLEEPQKAVTPGQAAVCYEDDVVVAGGWICRHAAVTTA"
     gene            2467..2790
                     /gene="cutA"
                     /locus_tag="VNW28_08770"
     CDS             2467..2790
                     /gene="cutA"
                     /locus_tag="VNW28_08770"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HXA10061.1"
                     /translation="MADEVLLVLSTFPDVATARRIGRQLVEEKCAACANILPQVESIY
                     WWEQKVESAHETLVFFKTTADRYEALQTTLRQLHPYDVPEIIAVRIGEGLPEYLRWVS
                     ETCPG"
     gene            2799..3884
                     /locus_tag="VNW28_08775"
     CDS             2799..3884
                     /locus_tag="VNW28_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HXA10062.1"
                     /translation="MIIREKEPANLEMPFGQLAGFFTPNEQFYIRAHFPVPEIDPANW
                     RLRLEGAVREARAWSLEDLRKLPATTVPATLECAGNSRVFLVPKVKGTQWELGAVGNA
                     EWTGVSLGEILRVAGLKEGALEVVLEGADSGPISDPPRPAGKIHYARSVPLGKALEGV
                     LLAYEMNGQPLTPSHGFPLRAVVPGWYGMAAVKWLQRIIVTDRPYNGYYQTVDYAFWQ
                     RDEAGVRLVPITEMQVKAAIARPGINEVIVAGTSYKVTGAAWTAEAEIVKVELSTDGG
                     SSWQTASLGKEKARHSWRLWEFDWSVPKESGRSVLMARATDSRGRTQPMGRDLDRGSY
                     EINHCLPIEVVIQASRLSGRAGVSPAR"
     gene            complement(3937..4479)
                     /locus_tag="VNW28_08780"
     CDS             complement(3937..4479)
                     /locus_tag="VNW28_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HXA10063.1"
                     /translation="MRDIVYFDLETQRTANDAGGWERKGNMQMSIGVTYSTRSGRYEI
                     FDEARVHDLIERLRRADLVVGFNVLNFDYHVLMGYTVLDLAHELPTADLMLDMERKLG
                     HRLGLDTIAQATLGVRKTAASLDAIRWWREGRLLEIAEYCCFDVKVTRLLHEHGCRHK
                     ELFFNDRFARKQRVAVDWKL"
     gene            complement(4539..5825)
                     /locus_tag="VNW28_08785"
     CDS             complement(4539..5825)
                     /locus_tag="VNW28_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10064.1"
                     /translation="MAGTGDPGYSFAHRMRIALLFVTLFALTSTQAQLTAPSPTVSPT
                     VSAPPSLPPRHVPLRFALPPLQGTISLGIYDGAGKLVRVLHREDAIADFTAGNDALET
                     EWDGNDEEGHPLPNGKYNARGYVVGDLKVEGIDYFFNDWVKDEKSPHIHRLDQLWMEN
                     GELRVDAELAGGKATAFVCDQATGAILREVAPTGGTHCNQVPAPPNIVHAIDCAAGKD
                     GTTWFVDALDENKSREVKQLSKQHEVLRRLDYVGDDPRPERIEASTSSEKIFLVEQSR
                     ILQRLRGLALVRILSNAAEGPVSDWKSLFDKKIIEHQNFSLVSGRPVAASANTAGELE
                     KVTQSLVPNPLQHDQPGKIELAVGLDADGSFLRTSDGLPLRTISETPNLTRTLINSNG
                     SKSVDVFQDDGAVVEQYRVSGLDQMVAFDCGEFELK"
     gene            complement(5836..7416)
                     /locus_tag="VNW28_08790"
     CDS             complement(5836..7416)
                     /locus_tag="VNW28_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HXA10065.1"
                     /translation="MPSASLALILHAHLPFVRHPEHDDFLEEDWLFEAITETYLPLLR
                     LLRRLARDEVAFRLTISLTPTLCAMLRDELLQQRYLRHLERLNALAHQEIERHRNDPP
                     LLELAKFYAEFFADARVFFEETLRRDVVRAFRELQDAGFLEIIASAATHAFLPLLQDF
                     PEAQRAQIRLGCDSYRAMFGREPSGFWLPECGYQHGLDSLLQEANIRWFVLDTHGLMF
                     ARPGPRHAIFAPCYTPAGPAAFARDHESNREVWSAESGYPGDPVYRDFYRDIGFDRTE
                     EELRPFLRPAGIRKFSGIKYHRVTGRTDEKELYHPAWAMAAADAHADDFLAKRRACFR
                     ELKEMNFDPIVVCPFDAELFGHWWFEGPRFLESFLRRAASEQRDFQLATPSAFLAAHP
                     SQQVVRPNPSSWGEEGFSSVWLEECNAWIYPHLHAATRRMTEIARRPHDTTAPTIERA
                     LQQAARELLLAQASDWAFLIKTRSAPEYATQRTKDHLLRFNRLYEQLSAGQIDLEFLG
                     QCELRNNIFPGLNWRYYL"
     gene            complement(7504..8667)
                     /locus_tag="VNW28_08795"
     CDS             complement(7504..8667)
                     /locus_tag="VNW28_08795"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXA10066.1"
                     /translation="MEISTRASQLTPSLTLSIDSKAKAMKSEGIDICGFGAGEPDFDT
                     PEHIKAAAIAALEGGFTKYTPSSGLPELRQAISEKFAADNGLEYRPSQIIVSNGAKHS
                     CYNAILATCEAGDEVIIPSPYWVSYPDMVRLAGAEPVMVQTSERHGWKMTAEEFENAM
                     TPRTKMVIMNSPGNPTGSVYTREELAAIVEVAAEEDIYILSDEIYEKLVYDDAKHVSI
                     ASLSKPAYELTITVNGFSKSYAMTGWRLGYMGAPEAIAKAVDSIQSHSTSNPCSFAQK
                     GAVAALKGDQQPLSDMHDEFDMRRNYMFDRISKIANITAVKPQGAFYVLVNISQLGLS
                     SQNFSDRLLSKANVAVIPGVAFGDDRTIRLSYATSIDVIKKGLDRFQDFCRTL"
     gene            complement(8713..9789)
                     /locus_tag="VNW28_08800"
     CDS             complement(8713..9789)
                     /locus_tag="VNW28_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXA10067.1"
                     /translation="MQAEELSYVIVTPHSMRKSRTGGIVARLISRTGLDLVGGRMFAP
                     GEELVRRYTEGIVTAQDPRHRATQVLIRDYVLKNFTGLRYGQRPRVLLLVFRGLGAVE
                     KIRHVVGHIVHERTSGETIRDTYGDYITDEAGKVTYFEPGVLASLDAESVEENLKLWA
                     EFSDRDGGLLDGVISYPTGTKVEKSLVLIKPDNFKFPNVRPGGVIEVFSRTGLYIIGF
                     KVHRMSVAQAEEFYGPVLAVLEDKLGAVKGREAWEDIVEFMSGGRPSTTSDEQRLAPG
                     TEKCIAIVYQGEDAVRKIRDVLGPTDPKKAPPGSIRKEFGQTIMVNAAHASDSAENAK
                     HEMEIVQVRENNLKPLIENFYARP"
     gene            complement(9887..>10569)
                     /locus_tag="VNW28_08805"
     CDS             complement(9887..>10569)
                     /locus_tag="VNW28_08805"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HXA10068.1"
                     /translation="TRTQAARLMRDFQGSAKQLPHDWRQEYLSPQIHAPVRVGKRLLV
                     VAETIPSDRPQLIVPAAGAFGTGEHATTAMCLRLLEEATRKSSPGWRLLDAGTGTGIL
                     ALAARRFGAGAVLGLDSDPVAVAHARQNARLNHISRVRFLAGDVLTWKPVAPYDLITA
                     NLFSELLIAALPIFRRTLRSKGRLIVSGILRAQAASVVHALGCSQFRLKQQRRRGKWV
                     ALLASRKT"
BASE COUNT         2002 a         3122 c         3433 g         2012 t
ORIGIN      
        1 accctgagtg tgaaaagtcg agtcggtgag ccgagcgaag tcgggtggct gattgccggc
       61 gcaatcatcg aagcgctggc cgatcgcgac ccggccatca atggcgtatt tcatggtttg
      121 ttcgatgacc agcacgcttt cgagaaaggg ccaaaaacgg tgggcgatat ctgggagatt
      181 ttgccctatg aaaattttct cgtcacgggc gagctcctgc cgatcgagct gaaggtggtg
      241 atggaagagg tgttcgagag ccgcgaaacc cgcagtctcg ccggcttcga aatgcggacg
      301 gagggaaagg ggagtgagcg ccgcctaacg agcctttgcc tggccgagag gcagccgctc
      361 gatcctgcga aacggtatcg catcgctctc aacactttcg acgcgcgcag cgccgggcat
      421 cgcttcatga agttgcgtag tctcctcgag cggtcggcgg cgaagtgcgc gtttcatccg
      481 gtgcaaacgc gcgacgcgct gatcgattat tttcggcgac acaaagtcgt gtatcgacat
      541 ccgcgcgagc gtccgccggc accgctcgtc atcccaggcg cggtcgagag atcctggggc
      601 acaggctttg aggtaacttc cgcgggatct ctcgactccg ctgcgcttcg ctcgggatga
      661 cgggccggcg ggcgcgaggt attttgcgaa cggcttattt tcccgacgtg ctgcctgatg
      721 cctttttcaa aaccgacccg ctccagtgcg cgaaaaacct gatcggcacc cagctgatct
      781 gggagaaatg cgccggcctg gtggtcgaga cggaggcgta tctggtaaag aacgacgaag
      841 cctctcacac cttcaggcga ccgagcacgc gtgcctttat cgagcggaac aagccgggcg
      901 cggcctacat ttatttcaac tacggggttc attggatgtt gaacgtgctc gtgaagggcg
      961 gcccgcgcga cggtctcatt cttatccgcg cgctcgaacc gcagcacggt ctggcgatca
     1021 tgcgcaagcg gcgcggcgtc gaggacgtgc gggaactttg ttccggcccg ggaaaactga
     1081 cccaggcgct cgcgatctcg cagcgacacc acgagatcga tctttcctcc gatccggcac
     1141 attgttttgg tccgcgaccc gatccgcacg tcctggtcgt ggccgacccg cggatcggga
     1201 tcagtcgagc gcgcgatttc ccatggcgtt ttaccctgcg cgacagcccg ttcgtcagcc
     1261 ggccggtgca aaaggccgcg cgttgaccag cccggcaatc gcgctgaatt taggcgctcg
     1321 atgaacggaa agaaagagcg cgtcctgctc ggcatgagcg gcggggtcga ttccagcgtc
     1381 gcgggctatt tgcttcgcga gcagggatac gacgtcgtcg gcgtgacgat gaaggtctgg
     1441 ccgcaggatt gtctctcgcg ggccgaggac aagtgctgcg gtccgcaggc catcgccgat
     1501 gcgcgtggcg tggcccactc gttaggcatc ccgtattacg tggtcgacga agcggaccaa
     1561 ttcgagcgcg tcgtaatcga ttacttttcg agtgaatacc aggccggccg cacgcccaat
     1621 ccctgcgtga tgtgcaacga aaagctcaaa ttcggcagcc tctgggaaaa ggcgcgcgcg
     1681 ctcgattgcg cttacatcgc gaccgggcac tacgccatca tcgagcaccc tgccggggtc
     1741 accgaccccg gcgacaacgg cgccgattgg cccagccggg ccgtgctgcg caaaggtgcc
     1801 gacccgcgca aggaccagtc gtatttcctt ttcagcttgc gccaggccca gctccggcat
     1861 gcgcttacgc cgttaggcaa aatggcgaag ccggaaatcc gcgcgatcgc ccgatcgtta
     1921 ggcctgaggg tcgccgagaa ggtcgacagc caggaaatct gtttcgttcc ggggaacgat
     1981 tacagggcgt tcctgcgctc gcatctcggc gacacggaat ttcaacgcgg cgggatctac
     2041 gatctttccg gcaactttct cgcggaacac gaaggcatcg agctgttcac gatcggccag
     2101 cggaaaggct tgcccggcgg ctcaccgcga ccgcgttacg tggtcgacat cgatgcgatg
     2161 agcaatcgcg tcattgttgg cgaggccgag gatttgctcg tggaggaatt cgagatcgat
     2221 cggctcaact ggagcagccg cgctccttca gatgaaccgc gcgccgtcac ggtgaagatc
     2281 cgctacagcc atcccggcgc cgccgccacc ctgatcccgc tggcgggcga tcgcgcactc
     2341 gttaggctgg aggaaccgca aaaggcggtc acgcccggcc aggccgcggt ttgctacgag
     2401 gacgacgtgg ttgtcgccgg cggctggatt tgccgccacg cggcagtcac gacggcctga
     2461 cgatccatgg cggacgaagt cctgctcgtc ctgagcacct ttccggacgt cgccacggcg
     2521 cggcgcatcg ggcgtcaatt ggtcgaggaa aagtgcgccg cctgcgccaa cattctaccg
     2581 caagtcgagt cgatctactg gtgggagcag aaagtggaga gcgcgcacga gaccctggtt
     2641 ttcttcaaga cgaccgccga ccgctacgaa gcgctgcaga cgaccctgcg ccaactgcat
     2701 ccttacgacg tgccggagat tattgcggtc cggatcgggg aggggctgcc ggaatatttg
     2761 cgctgggttt ccgagacctg cccgggatag ttaagaacat gatcattcgc gaaaaagagc
     2821 cggccaattt ggaaatgccc ttcggccaac tcgcgggctt cttcacgcct aacgagcaat
     2881 tttacatccg ggcgcacttt cccgtcccgg agatcgatcc ggcgaactgg cgactacggc
     2941 tcgaaggcgc ggtcagggag gcacgtgcgt ggagtctgga agatctgcgc aagctgcccg
     3001 cgacgaccgt cccggcgacg ctggagtgcg ccggcaacag tcgcgttttt ctcgtgccca
     3061 aggtgaaggg aacgcaatgg gaactgggcg cggtcggcaa cgccgagtgg accggagtgt
     3121 cgttaggcga aatcctccgg gtggccgggc tgaaagaggg cgcactcgaa gtcgtcctgg
     3181 aaggcgcgga ctcgggcccg atcagcgacc cgcccaggcc ggcgggcaag attcattacg
     3241 cgcgcagtgt tccgttaggc aaagcgctcg agggcgtcct tctcgcctac gagatgaatg
     3301 gccagccgct cacgccttca cacggcttcc cgcttcgcgc ggtcgtgccg ggctggtacg
     3361 gaatggcggc agtgaagtgg ctgcaacgca tcatcgtcac cgaccgcccc tacaacggct
     3421 attaccagac ggtcgactac gccttttggc aacgcgacga ggccggggtg cgcctcgtcc
     3481 cgatcacgga aatgcaggtc aaagcagcca tcgcccggcc ggggataaac gaagtcatcg
     3541 tcgcgggcac gagctacaaa gtgacgggcg ccgcctggac ggccgaggcg gagatcgtca
     3601 aggtcgagct gagcacggat ggggggagtt cctggcaaac ggcgtcgtta ggcaaggaga
     3661 aggcgcgcca cagctggcgg ctttgggaat ttgattggag cgtgccgaag gagtcgggcc
     3721 gcagtgtgct catggcgcgc gcgaccgatt cgcgcgggcg cacccagccg atggggcgcg
     3781 atcttgatcg cggcagctac gagatcaacc actgcctgcc gatcgaggtc gtaatacagg
     3841 cgtcccgcct gtcgggtcga gcgggcgtct cgcctgctcg ttagggcaac gtgcgggcgg
     3901 gacgcccgct cgacggaaca ggccagaggc cctgtgttac agcttccagt cgactgcgac
     3961 ccgctgtttt cgggcgaagc gatcgttaaa gaaaagttcc ttgtggcggc aaccgtgttc
     4021 gtgcaagagc cgcgtcactt tcacatcgaa acagcagtat tccgcaatct ccaagaggcg
     4081 accttcgcgc caccagcgaa tcgcgtcgag actcgccgcg gtcttgcgca cgcctaacgt
     4141 cgcctgggcg atcgtgtcaa ggccgaggcg atgcccgagt ttgcgctcca tgtcgagcat
     4201 caggtcggcc gtcggcaact cgtgcgccag atcgagcacg gtgtagccca tcaggacgtg
     4261 ataatcgaaa ttaaggacgt tgaaaccgac aacgagatcg gcccggcgca ggcgttcgat
     4321 caaatcgtgg acccgcgctt cgtcgaaaat ctcatagcgg ccgctgcgcg tgctgtaggt
     4381 gacgccgatc gacatttgca tgttgccttt gcgttcccag ccgccggcgt cattcgcggt
     4441 gcgttgcgtt tcgagatcga agtagacgat gtcgcgcatc agggaagttg agagttgggc
     4501 gacgagagtt gagagacgaa gcgcagaacg cgagtctatt atttcagctc aaattcgccg
     4561 caatcgaaag ccaccatctg gtcgaggccg gagacgcggt attgctccac cactgcgccg
     4621 tcatcctgaa agacgtcaac ggatttgctg ccgttggaat tgatcagcgt ccgcgtcagg
     4681 ttcggcgtct cgctgatcgt gcggagtggc agaccatcgc tcgttcgcag gaagctgcca
     4741 tcggcgtcca gcccgacggc cagctctatc ttgccgggtt gatcgtgctg caaaggattt
     4801 gggaccaggg attgggtgac tttttccagc tcgccggccg tgttcgcgga cgctgcgacc
     4861 ggcctgccgc tcacgagcga gaaattttga tgctcgataa tttttttgtc gaacaggctt
     4921 ttccagtcgg aaaccggccc ttcagccgcg ttgctcagga tgcggacgag cgccaagccg
     4981 cgcaatcgct gcaggattcg gctttgctcc acgagaaaga ttttttccga cgaagtagaa
     5041 gcctcgattc gctccggcct tggatcgtca cccacgtagt caagccggcg tagcacttcg
     5101 tgctgcttcg acaattgttt gacctcacgc gatttgttct catcgagagc gtccacgaac
     5161 caggtcgtgc cgtctttgcc cgccgcgcaa tcaatcgcgt gcacgatgtt tggaggggcc
     5221 gggacctgat tgcagtgcgt ccccccagtc ggggcgacct cgcgcaggat ggcaccggtc
     5281 gcttgatcgc agacgaacgc ggtcgctttc ccgccggcca gctccgcgtc gacccgcagc
     5341 tcgccgtttt ccatccagag ctggtcaagc cggtggatgt gcggtgattt ttcgtccttc
     5401 acccaatcat tgaaaaaata atcgatgcct tcgaccttca aatcgccgac cacgtaaccg
     5461 cgcgcgttgt acttcccgtt aggcaaagga tgcccctcct cgtcgttgcc gtcccattcc
     5521 gtttcgaggg cgtcattccc ggcggtgaaa tcggcgatcg cgtcttcgcg atgcaaaacg
     5581 cgcaccaatt tgccggcccc atcgtaaatc ccaaggctga tcgtcccctg caacgggggc
     5641 aaggcgaaac gcagcggcac atggcgcggc ggaagcgagg gcggcgctga gaccgtcggc
     5701 gagacggtcg gcgacggcgc agttaattgc gcctgagtgg aagtgagcgc aaaaagggtg
     5761 acgaaaagaa gagcgatgcg catgcggtga gcgaagctgt agccgggatc gccggtcccg
     5821 gccaacgaaa ttcggctaga ggtaatatcg ccagttgagt ccgggaaaaa tgttatttcg
     5881 cagttcacat tggccgagaa actcgaggtc gatttgtccg gcgctgagtt gctcataaag
     5941 tcggttgaaa cggaggaggt gatcttttgt gcgctgcgtc gcgtactcgg gcgcgctccg
     6001 ggttttaata aggaatgccc aatcgcttgc ttgcgcgagg agaagttcgc gcgcggcctg
     6061 ttgcagcgcg cgctcgattg tgggcgcggt cgtatcgtgc ggccggcggg cgatctccgt
     6121 catccgccgc gtggcggcgt ggaggtgcgg ataaatccag gcattgcact cctccagcca
     6181 gacgctgctg aagccctcct cgccccagct cgaaggattt ggcctaacga cctgttgcga
     6241 cggatgcgcg gccagaaaag cggacggcgt ggcgagttga aaatcgcgct gctcgctggc
     6301 cgcccggcgg agaaagctct cgaggaagcg cggtccctcg aaccaccaat ggccaaaaag
     6361 ctcggcgtcg aatggacaca cgacgatcgg atcgaagttc atttctttca gctcccggaa
     6421 acatgcgcgg cgtttggcga gaaaatcgtc ggcgtgcgcg tcggccgcgg ccatggccca
     6481 cgcggggtga tagagttctt tctcgtcggt ccggccggtc acgcggtgat atttgatccc
     6541 gctgaatttc cggatgccgg ctggccggag gaaagggcgg agctcttctt ccgtgcggtc
     6601 gaaaccgatg tcgcgataaa aatcacgata aaccgggtcg cccggataac ccgactcggc
     6661 actccagacc tcgcgattcg attcgtggtc gcgcgcgaaa gccgccgggc cggcgggcgt
     6721 gtagcagggg gcgaagatcg cgtggcgcgg cccaggccgg gcaaacatca ggccgtgggt
     6781 atcgaggacg aaccagcgaa tattcgcttc ctggagcaga gaatcgaggc cgtgctggta
     6841 gccgcattcc ggcagccaaa aaccgctcgg ctcgcgcccg aacatggcgc gatagctatc
     6901 gcagccgagg cgaatctgcg cgcgttgcgc ctcgggaaaa tcctggagca gggggagaaa
     6961 ggcgtgcgtc gcggcgctgg cgatgatctc gagaaacccg gcgtcctgca gttcgcgaaa
     7021 cgccctaacg acatcacggc ggagcgtttc ctcgaagaag acacgcgcgt cggcaaaaaa
     7081 ctccgcgtag aatttcgcca gctccaggag cggcggatca ttccgatggc gctcgatttc
     7141 ctggtgggcg agcgcgttca gccgctcgag gtgtcggaga tagcgctgct ggagtaactc
     7201 gtcgcggagc atcgcgcaga gggttggtgt gagcgagatc gtgagccgga aggccacttc
     7261 gtccctggcc agccggcgga gcagccggag gagcgggagg taggtctcgg tgatggcttc
     7321 gaagagccag tcttcctcga gaaagtcgtc gtgttccggg tgcctgacga aaggcaaatg
     7381 cgcgtggagg atgagcgcga gcgaggcgga tggcacgtgt catcccgggc gcagccgagg
     7441 gatcccgctg cgctggctta aaggtttctc cacgggatcc ctcgactgcg ctcgggatga
     7501 cgtctagagc gtgcggcaga aatcctggaa ccgatcgaga ccctttttga tcacgtcgat
     7561 actcgtcgcg tagctcaggc ggatggtgcg atcgtcgccg aaagccaccc cgggaatgac
     7621 cgcgacgttc gccttgctga gcagacgatc agagaaattt tgcgagctca acccgagctg
     7681 gctgatattg acaagaacgt agaacgcgcc ctgcggtttg accgcggtga tgtttgcgat
     7741 cttcgagatg cggtcgaaca tgtaattgcg ccgcatatcg aactcgtcgt gcatgtcgga
     7801 gagcggctgc tggtcgccct tgagcgcggc gaccgcgcct ttttgcgcga acgagcacgg
     7861 gttcgaggtg ctgtggcttt ggatggaatc gaccgccttg gcgatcgctt ccggcgcgcc
     7921 catgtaaccg agccgccagc cggtcatcgc gtaggatttg ctaaagccgt tgactgtgat
     7981 cgtcagttcg taggccggct tcgagagcga cgcgatgctg acgtgcttcg cgtcgtcgta
     8041 aacgagcttc tcgtagattt cgtcggacaa aatgtagatg tcttcctcgg cagcgacctc
     8101 gacgatcgcg gccagctcct cacgggtgta aaccgagcca gtcgggttgc ccgggctgtt
     8161 catgatcacc attttggtcc gcggggtcat cgcgttttcg aattcctcgg cggtcatttt
     8221 ccagccgtgg cgctcgctcg tttgcaccat caccggctcg gcgccggcga gccgcaccat
     8281 gtcgggatag ctgacccaat acggcgaggg aatgatcact tcgtcgcccg cctcgcaggt
     8341 cgcgaggatg gcattgtaac aggaatgttt cgcgccgttg ctcacgatga tctggctcgg
     8401 ccgatactca agtccgttgt cagccgcgaa tttctccgag atcgcctggc gcaattcggg
     8461 caacccggag ctgggcgtgt atttcgtaaa gccgccctcg agtgcggcga tcgccgccgc
     8521 cttgatgtgt tccggtgtgt cgaagtcggg ctcgccagcg ccgaagccgc aaatatcgat
     8581 gccttcgctc ttcatcgcct tcgccttgct gtcgatcgaa agggtgagcg agggagtgag
     8641 ttgagatgcg cgggttgaga tttccatggc caaagtattc atcatgaggt ttcgaaaaaa
     8701 ggggagcgca agctatgggc gagcgtagaa gttttcaatc aaaggtttca ggttgttctc
     8761 gcgcacctgc acgatttcca tctcgtgctt cgcgttctcg gccgaatcgg aagcgtgcgc
     8821 ggcgttgacc atgatcgtct ggccgaattc cttgcgaatc gagcccggcg gcgccttttt
     8881 cggatcggtc gggccaagca cgtcgcggat tttgcgcacc gcgtcttcgc cctggtaaac
     8941 gatcgcgatg catttctcgg tgcccggcgc cagacgttgt tcgtcgctcg tcgtgctcgg
     9001 ccggccgccg gacatgaatt cgacgatgtc ttcccaggcc tcgcgtcctt tgacggcgcc
     9061 gagcttgtct tccagcacag ccagcaccgg cccgtagaat tcctccgcct gggccacgct
     9121 catccggtgc accttgaagc cgatgatata aagcccggtg cgggagaaga cctcgatcac
     9181 cccgcccggc cggacattcg gaaatttgaa attatccggc ttgatcagga cgagcgattt
     9241 ttccaccttc gtcccggtgg gatacgaaat cacaccatcg aggaggccgc cgtcgcgatc
     9301 cgaaaactcc gcccagagct tcagattctc ctcgaccgac tcggcatcga gcgacgcgag
     9361 cacgcccggc tcgaaatagg tcaccttgcc cgcttcgtcc gtgatgtaat cgccgtaggt
     9421 gtcgcggatc gtctcgccgc tggtgcgttc atgcacaatg tggccgacca cgtgccgaat
     9481 tttttccacc gcgccaaggc cgcgaaaaac caggagcagg acgcgcggac gctgaccgta
     9541 gcgtaggccg gtgaaatttt tcaggacata atcgcggatc aatacctggg tggcgcgatg
     9601 ccgcggatcc tgcgcggtca caatcccctc ggtgtagcgc ctaacgagtt cctcaccggg
     9661 ggcaaacatg cgcccgccga ccagatcgag cccggtgcgg gagatgaggc gggcgacgat
     9721 gccgccggtg cgcgatttgc gcatcgagtg gggcgtgacg atgacgtagg agagttcttc
     9781 ggcctgcata agggatcctg agcggctgaa cggattaagc cgcagggcgc aggcaatcga
     9841 agtcgctcgg acggacggaa ggtagctgcg gctaacgagc gggcaactag gttttccggg
     9901 aggcgagaag tgccacccat ttcccgcgac ggcgttgttg cttgaggcgg aattgggagc
     9961 agccgagtgc atgcactacg gaggcggcct gcgcccgcag gatgccggaa acgatgaggc
    10021 ggcctttcga tcgaagggta cggcggaaaa tgggcagcgc ggcgattaac agttcgctaa
    10081 agaggtttgc ggtgatgagg tcgtaaggcg cgactggttt ccacgtcagg acatcgccgg
    10141 cgaggaacct cacccgcgag atgtgattga ggcgcgcgtt ctggcgggcg tgagcgacgg
    10201 cgacggggtc gctgtcgagg ccgagaacgg cgcctgcgcc gaatcgccgc gcggcaagag
    10261 ccagaatgcc ggtgccggtc ccggcgtcga gcaggcgcca gccgggagaa gattttcgcg
    10321 tggcttcctc gagcagccgc aggcacatcg cggtcgtcgc gtgctcgccc gtgccgaacg
    10381 cgcccgcggc cgggacaatg agctgcggtc ggtcgctcgg gatggtttcc gcgaccacca
    10441 gtagacgctt gccgacccgg acaggcgcat ggatttgggg tgacaaatac tcttgtcgcc
    10501 aatcgtgcgg caattgcttc gcggagcctt ggaagtcgcg catcaaccgt gcagcctgag
    10561 tgcgggtgg
//
LOCUS       DATMNS010000252        21764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2014398, whole genome shotgun sequence.
ACCESSION   DATMNS010000252 DATMNS010000000
VERSION     DATMNS010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 21764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21764
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014398"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VNW28_08810"
     CDS             <1..464
                     /locus_tag="VNW28_08810"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXA10069.1"
                     /translation="VSDFAVGTLVAVDGKLIFGHFPNVVARDQATGALLWSVPHYGSA
                     DQGSPAVADGLVFVETNLSNVVALDENTGNLVWTGSARSPSGPSVANGVVYASNYSGE
                     WDAYNETDGTLLWSVTNSDGCFGPCANTVPIVSRGMLYLAEGQLRAYKVPN"
     gene            complement(515..1540)
                     /locus_tag="VNW28_08815"
     CDS             complement(515..1540)
                     /locus_tag="VNW28_08815"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXA10070.1"
                     /translation="MPNRFFTAAIEVDPELIPGARNAIRDCLRLQPGERITIITDEAT
                     REIAAALQSEVELVGSVYAVFVLEECARRPLTTMPQVILDDLATSQVSIFCAQSQFGE
                     LSSRMQMSEVVNRHRIRHGHMVNISRQIMLEGMRADFAAIDAHSQLLVEQARRTKRVT
                     CRTPHGTDFVAELSPKLKWIKTSGIITPDKWGNLPGGEIFTSPGNTNGTFVVDGVVGD
                     YLCQKYGDIQKTPLTIVVKENRIHSLVCDNRELLEEFRAYTSTDENSNRVGEFAVGTN
                     TACTRVIGNILQDEKIPGIHIAFGHPYAEHTGQTWISKTHIDCVGRDFDIWFDDEQIM
                     ARGKFLV"
     gene            complement(1591..2739)
                     /locus_tag="VNW28_08820"
     CDS             complement(1591..2739)
                     /locus_tag="VNW28_08820"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HXA10071.1"
                     /translation="MSAADHVFTLGIEEEFQIIDPETRELRSHIQQILGEGKVTLNEQ
                     IKPEMHQSVVELGTDVCGDIHDARRQVLRLRSELAAMAGEGGMKIASAGTHPFSHWFD
                     QHITEGERYAEIVKDMQQVARANLIFGLHVHVGVPDRAIAIHVMNQARYFLPHLYALS
                     TNSPFWVGRNTGLKGYRLKVFERFPRTGTPDAFESLSEYEDFLKLLIKTGCVDNAKKI
                     WWDIRLHPFFDTLEIRICDAQSRVDDTLALAAVIQAIVAKLHKLLYHNTTFRVYRRRL
                     IDENRWRASRYGLDGRMIDFGREAEVDTRKLLGELLDFIATEVDELGSGAEIAHVEKI
                     MREGTGADRQIAAWAGQHNMHDVVDLIVNETYEGLTVPQRRAATEVQA"
     gene            2888..3322
                     /locus_tag="VNW28_08825"
     CDS             2888..3322
                     /locus_tag="VNW28_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXA10072.1"
                     /translation="MQLSLHPRHHGFTLIEIMIVVSLIALLAMIAVPSFLRARQRAEA
                     VAVLNELRLLDAAIAQYAVDNNKTTGAEITIVDLQPYLKKGGALYNTGQDVLGNDFGT
                     FAVDSLPLVPTATWRALADVADKAFWSPYYQDQVAPADPSTR"
     gene            complement(3445..6198)
                     /locus_tag="VNW28_08830"
     CDS             complement(3445..6198)
                     /locus_tag="VNW28_08830"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXA10073.1"
                     /translation="MRPSFAIRGNLDLIDDNYRRWEKDPESVDSSWRAFFEGFELGNT
                     PQRNGAAVAAPPRAEPGESPLQTRVDGLVYAYRTLGHTIARVNPLAEHRPENPLLTLR
                     ELGFSEKDLDLQVASKFFLDNKKMTLRAMRATLDEIYADAIGTEFQHIQNPRIRNWVR
                     DRLETRPAKHQASTQVEIALLRLLLESEAFEVFLHTRYVGQKRFSLQGAESLMVILDT
                     ILHRSPSAGIEEICMGMAHRGRLNVLANFLQKSLKVLFTEFSTNYIPDLVAGDGDVKY
                     HLGYHTIRQLASGAEIEIRLAANPSHLEAVDPVVEGTARARQRIRGDTEHRRKVLPLL
                     VHGDAAFAGQGIVAETLNMSQLHGYSTGGTVHVVVNNQIGFTTLPEDARSSTYATDIA
                     KMIEAPIFHVNGDDPIAVRFVTETALDFRQEFGRDAVIDMYCYRRYGHNEGDEPLFTQ
                     PDLYARIANRPSVAQLYKRELIETSVLSAEAAAQLEKDLQDRLEGALEEVKALERSRE
                     GRRDRFNESTAVFQPKYSGKSRPTAISPELLKQIVDGLTRVPDDFNVLPKVKRITIDR
                     QREAYETGGPYDWGFAEALAFGSLLLEGVPVRLSGQDSRRGTFSHRHSFLYDAKSGQP
                     YLPLLHLAPNQARICIYNSLLSEAAVLGFDYGYSLDYPNMLCLWEAQFGDFANGAQTI
                     IDQFIVSAESKWQRPSGIVLLLPHGYEGQGPEHSSARLERFLQACAEDNIQVCNLTTA
                     AQYFHVLRRQMKRDFIKPLIIMTPKSLLRFEQASSRTEDFTAGSFSEILGAPEVAPPE
                     KIERVILCSGKVYYDLLNHREAEKIASAAIVRLEQLYPLAEEKLRAVLAPFSRAQKVV
                     WCQEESQNMGAWTYLEPRLRAMLGREILYAGRNASASPAVGALAVHKREQACVINDAF
                     TLQ"
     gene            6291..6827
                     /locus_tag="VNW28_08835"
     CDS             6291..6827
                     /locus_tag="VNW28_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10074.1"
                     /translation="MTAEEVYESVTNGGTSDFAAAVAIFDRFGPGSWCLIGGLAVNCY
                     VEPVYTVDADFVMVAREMGKVQAALTAAGFALQTFPHSVNARRSGSKLSLQLTTDARY
                     QDFLGRAERREVLGLRVPVATLDDVVQGKVWAWQDEERRLTKRKKDELDLLRLGEAYS
                     HLSAVLPPEIVRQLETKS"
     gene            6824..7996
                     /locus_tag="VNW28_08840"
     CDS             6824..7996
                     /locus_tag="VNW28_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXA10075.1"
                     /translation="MKITRRETASSGVLGAALGFLSLLRLSSAVFAQADPASEPSVLA
                     VAKVLPAVVNINTERTVRRQVRDPFDDFAAQFFGAYRNRPREIRQRLQSLGTGFLVDP
                     AGYIVTNEHVVERAADLKIEVITNDNKSFKARYITGDAKKDLAFIKIDSDQPLPFVNL
                     HDISPNLLGQTVIVVGNALGYGSSISRGVLSGVKRDITIDNVDYHNLLQTDAAINPGN
                     SGGPLIDLSGRLVGISSAKMAFTPQGIPTQGLGFAIPAEIVRQNVEQFKKIAEKQPPP
                     KPGATEEQTGSSNADRLFGLQLQDLTDDLAAALGLASGKGVLISAVEPDSPADEAGIE
                     RGLVLFRIGKYEVNSVKEVEKLLAQADSGSSVDFALGIIGRGGRGRRIETVNLEAR"
     gene            8060..8995
                     /locus_tag="VNW28_08845"
     CDS             8060..8995
                     /locus_tag="VNW28_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA10076.1"
                     /translation="MIKVENLTKRYAGQTAIDSLNFEVGKGEIMGFLGPNGAGKSTTM
                     RILSSFMPPTSGRASVAGFDIFEKSLQARAHLGYMPENVPLYNDMRVTEYLDYRAALK
                     GVPHRRINERVGDVKELCGLKDVEKKLIGALSKGYRQRVGLADALVHEPDLLILDEPT
                     SGLDPNQIRQVRELIKNLGRQHTILLSTHILPEVEMTCSRVIIINKGRIEACDTPENL
                     LNQMRTATGVLLEAQTGTDNGVEELKKISGVKDVNLTTEGDWQIFSLRAEAGAEVRPE
                     VFRLASERRWKVRELSQRKATLEDVFVEITHADEI"
     gene            9151..9906
                     /locus_tag="VNW28_08850"
     CDS             9151..9906
                     /locus_tag="VNW28_08850"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA10077.1"
                     /translation="MRKFFTLLSREIRSYFYSPIAYIVLVFFLLVSGVDFYFQVSFMN
                     QRAIPYTVQEAFFNSVFFWFAFVLIFPLITMRLFAEEFKLGTIEPLMTAPVRDWQVVL
                     SKYFGAFFFYIVLWIPTGLYFVIFQALTHQPAAGSTGAYLGGYLMLLLLGMFYLSIGC
                     FASVLTKNQIVAAIISFATITLLFFLGLVSFILLDISSDTRQLLGYFSAIEQMGTLSR
                     GEIDTRPMVLYLSMTIVLLTFTYQAFQTRKWRL"
     gene            9910..11406
                     /locus_tag="VNW28_08855"
     CDS             9910..11406
                     /locus_tag="VNW28_08855"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HXA10078.1"
                     /translation="MADETSSPKKAIKIHRFQIGLNVILQVTIIIVLAAMVNYLGFEH
                     YRRWDLSRDKKYALSDKTKNFLDSIKGKVRITVFFGSQDPITSDVQNLLTEYQYASHG
                     KIDVEYVDPERNLTRAKELFDKYKVVTDESVLIVDYQGRSKTVKASEMADMDQGNPLA
                     GEQPKVAAFKGEQAITAALIDIVEGKKNIVGWVTGHKEPPLSGESPVSVLKTFIENEN
                     ITLRELNLFEVPAIPADLKAIVIAGPQYDFSDREMKMLREFWNKDGRILLLLDPAAKT
                     PKLLAFLGELGVKEDDDRLMAMVKTGIEEVARVRDVVAHFLSDNPVTKRLADVHAPFF
                     GSTSSLTLESQQVAAANIHLAPLAQAEKGYWGETDYYSNDEALLQFDPAKDKGEPLTI
                     AASVEKGGSADERVQINSARLVVVANSTFIQDNALTQDQQGLDFVSASVNWLLSREQM
                     IGIAPKIPQTLTFVLDEKALASLRWIILVLMPLVPAILGFAVWWRRRA"
     gene            11419..13311
                     /locus_tag="VNW28_08860"
     CDS             11419..13311
                     /locus_tag="VNW28_08860"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HXA10079.1"
                     /translation="MKTRTTLLLLILTVGLGLWIKYFESKKPNTAEAQREAGNVVNFD
                     REKLEGIVIQNGDDRIELRRQNGKWRLTEPVKDQADGAAVDNLIGDIDTWRRDGTVPA
                     KEVTADKGRMNEFGLVQPKLRIRLIGPNAPPEILFGKDAALEGKMYVRLADSKDVIIA
                     AQSVRTDISKKPDDFRDRKLTDLTTAQVTRTVLKSPAGEIELAKKNEHWEIVKPLEAR
                     GDDQKVDDLLAQVTNARIQEFVADDRGDLHTYGLSEPRGSITIYGADDKSASRTGSSR
                     GEEGRTLQLGAVAEKMKDAIYARFLPRNAVYALPKKTEEILTVRPNDLRDRHLVRLDT
                     NNLDRINLEATGQPKIVLARKDQNWTISSRNNQPANGDEVRRLIDTLNDQQVTRFVAD
                     TASELPKYGLDHPQLRLTFSSFASENTAETAAGEHPFLTLSFGKVEGDDVYARVGEEP
                     FIVAVNHELLGQVWIEPTQWQELSVFKFAPNEIHRISRMTNHEESLVRSGPQSWQWSK
                     GEGAMDNADLQSLLNTLASLRAVRWVGATNPAQGFDKPTLVLIFTTSPDDKATHKLTI
                     GHRTDDAMWFAKVDGRDGTFVVSDPDFGAFKLPLTKATAASPTPVATPIGRVPSSATA
                     APSPGE"
     gene            complement(13312..14013)
                     /locus_tag="VNW28_08865"
     CDS             complement(13312..14013)
                     /locus_tag="VNW28_08865"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HXA10080.1"
                     /translation="MNHLPISNLVDRDPEKVRAMFGRVARRYDLANHLLSGGLDFWWR
                     DRASEIVRGWNPRRVLDLATGSGDLALTIAQKLPQAEITGADFCAEMLATARAKGLTR
                     TIVADALHLPFPAARFEVVTVAFGLRNMADWRAALAEMARVLVPGGHLLVLDFSLPRG
                     FLRPIYRFYLHRCLPWLAGLVTGEKQAYDYLGASIEKFPSGPAMCELMRASGFRAANA
                     QPLSGGIVTIYCASA"
     gene            complement(14144..14641)
                     /locus_tag="VNW28_08870"
     CDS             complement(14144..14641)
                     /locus_tag="VNW28_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10081.1"
                     /translation="MSDLKHLSREAIPAALEKAERYRLLNEPAEAESICLDVLHAEPE
                     NQEAIITLLLALTDRFSKGYGVSDAQAGQLLARVKGDYERAYYMGILAERRAKAKLAQ
                     GTPGAHHYAYHVLREAMEFYEKAEAIRPPGNDDALLRWNTCARIIAKNKLVPRDEDKS
                     EPPFD"
     gene            complement(14634..15422)
                     /locus_tag="VNW28_08875"
     CDS             complement(14634..15422)
                     /locus_tag="VNW28_08875"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HXA10082.1"
                     /translation="MKTATFRIWRGNAAGGEFRDYETGVSEGTVVLDAIHHIQATEAN
                     DLACRWNCKAGKCGSCSAEVNGMPRLMCMTRLSELPLDKPVTVEPIKAFPLLKDLITD
                     VSWNFRVKKKIRKFKPRPPDAPDGTWRMQQEEIDRVQEFRKCIECFLCQDVCHVLRDH
                     RKYDEFIGPRFLIYVAALEMHPLDTEDRLEELRATQGIGYCNITKCCTKVCPESIHIT
                     DNAIIPLKERVVDDYYDPLGWLWKIVRRRSQRVSSSSPHEPDHV"
     gene            complement(15628..17439)
                     /locus_tag="VNW28_08880"
     CDS             complement(15628..17439)
                     /locus_tag="VNW28_08880"
                     /inference="COORDINATES: protein motif:HMM:NF005866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HXA10083.1"
                     /translation="MPDYETFTHDVLVIGTGGAGLRAAIEASAAGVSVGMVSKCLLGK
                     AHTVMAEGGIAAALANVDDRDNWKVHFADTMRGGQYVNNWRMAELHAREAPERVRELE
                     AWGAVFDRTKDGRILQRNFGGHKYPRLAHVGDRTGLEMIRTLQDHGIHQGIDVHMEVC
                     VLALLKDGARISGAFGYDRERGRFRIFRANAIILATGGIGKAYKITSNSWDCTGDGHA
                     LAYHAGAELIDMEYIQFHPTGMVWPPSVRGMLVTEGVRGEGGILTNNDGQRFMFDFIP
                     ENYKAQTADNEEEGWRYTQGDKEARRPPELLTRDHVARCIVREIKEGRGTPHGGVYLD
                     ISWIKKRLPNAEEHIKRKLPSMYHQFKQLGDVDITREPMEVGPTTHYIMGGVRVDGDT
                     QMSSVPGLFAAGECAAGINGANRLGGNSLSDLLVFGQRAGEFAAKFAQGQSLGEIDEA
                     IVKKLEAEALQPFANRDQDNPYTVQRDLQEMMQHNVGIVREESEMQSALEHLKVLWER
                     AREVGVTGHRDFNPGWHTALDLQNLLTVSEAITRAALERKESRGAQFREDYPEKSAEF
                     GNVNTIVWKGEDGGMRIRRDPIPEMREELKRVIEEMG"
     gene            complement(17452..18255)
                     /locus_tag="VNW28_08885"
     CDS             complement(17452..18255)
                     /locus_tag="VNW28_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10084.1"
                     /translation="MTQAELTLSRRSFGQTMRADIWWAQPLVVFLGLGAFVVYSTWAA
                     LQGRNYYFGPYLSPFYSPEIFGASPHSWFGAKPVWWPAWLIFSPSLLVLWAPGGFRLT
                     CYYYRGAYYKAFWADPPACTVGEPRSTYLGERSFPLIMQNVHRYFLYLALLFILVLAH
                     DVWEALWFIDPATGRTSFGMGVGTLVLAVNVVLLGGYTFGCHSLRHLVGGFLDQFSKA
                     PACYRGYACVSCLNRRHMLWAWMSLFWVAFSDVYVRLCAVGVWHDLRFF"
     gene            complement(18335..20386)
                     /locus_tag="VNW28_08890"
     CDS             complement(18335..20386)
                     /locus_tag="VNW28_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10085.1"
                     /translation="MRTFFQELKRRRVYRVALAYVIGASATIQLVGTVFPAFHAPDWT
                     MQAFLVLVALGFPVALVLGWVFEVSHGGLRRTTDDGAGQATDYRRLLVLGVTGLLVAG
                     VAVAGYAVWHPWRKPPDTDRSSVIPEKSVAVLPFDNLSDDDENAHFAEGVHDEILTDL
                     AKVSDLKVISRTSTMQYRSGAERNLRDIGRELGAAHVVEGTVQRVGNRVRVSAQLIDA
                     RTDAHLWAEHYDRDLANVFALESDLAETIVAQLRSKLSPQEKAAMEVQPTSDPVAHDL
                     YLRANALMSGPLFNAQGTENLLKAVDLLEKAVARDPNYFLAYSRLARAHDQIYLTGPD
                     HTPARLALADAAIKAAQRLRPDAGETHLALAEHLYCGFLAYDEARQELEIARVSLPNE
                     PLIFELAGFIDRRQGRWDASTWNLAHALDLDPRDTYILQQLAVSYQLLRRYADAAGLL
                     DRALKIRPGDMSLRVARAGIDLHQRADPRPLHSTIEKIVTANPEAAAGLADEWLFLSL
                     CEREPDTADRAVAAMREEGYNNEGVLFSRPWCRALVARVRGDATGERAAFVAAREEVE
                     RSLREAPDQGQSLCVLAMIDAALGRKDDAIREGRRAVDLLPLDKDSINGALAIEYLAV
                     TYAWTGETDQAIEQLKRAAAIPSDVSYGQLRLHPFWDPVRGDPRFDQIVASLAPAAGK
                     H"
     gene            complement(20410..21363)
                     /locus_tag="VNW28_08895"
     CDS             complement(20410..21363)
                     /locus_tag="VNW28_08895"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HXA10086.1"
                     /translation="MARKWQWLGTFLFPPDSGCWLSLLRVGLGFQIILYAVSLRADWS
                     DLYGARGYGLITRALVEAAFSGENFYTPRLGWLVFVAKHVGLTEATTLSLVWVSLVVS
                     GLLLITGWSSRLAAGVSWFLYLCSVKSGAFFAYGVDNFTTIGLFYLMIAPLPDPWSLD
                     SRLRKRRRINPERLGFHRRVLQLHLCVIYFFSGISKSLGADWWNGNSVWRALTRPPFD
                     LVPPGLLMRFEYLLPAIGILVCLLETGYPVFIWMRRTRAVWLTGILGMHISIGLTMGL
                     YLFALIMIVLNLAAFGPDLICQFQNPFRVRKKTSDIPPLPV"
     gene            complement(21370..>21764)
                     /locus_tag="VNW28_08900"
     CDS             complement(21370..>21764)
                     /locus_tag="VNW28_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10087.1"
                     /translation="ANCSGIEVGYSYFAPVIPGNCKLVFELHYPDGRVEYDLPVVGGS
                     AAGGRVSALLDYLQVIHYLPLREALLRSLAESVRREHPDATLIRAVLGVANLVNADEY
                     RAGKRISYRALFAYDFRFHPKTRPPVLP"
BASE COUNT         4464 a         6639 c         6472 g         4189 t
ORIGIN      
        1 ccgtttccga tttcgcggtc gggacgttgg tggcagtgga tgggaaactc atcttcggac
       61 acttcccgaa cgtcgtcgcg cgagaccagg ccaccggcgc gctcctttgg tcggtcccgc
      121 actacggaag cgctgaccag ggttcccccg cggttgccga cgggttagtc ttcgtcgaga
      181 ccaatctctc aaacgtcgtc gcgctcgacg aaaacaccgg caatctcgtc tggacgggct
      241 cggcccgcag cccctcgggg ccatctgtgg ctaatggcgt cgtctacgcg tcgaattaca
      301 gcggggaatg ggatgcctac aacgagaccg acggcacgct gctttggtca gtcaccaaca
      361 gcgacggctg cttcggccct tgcgccaata ctgtcccgat cgtctcccgc gggatgctct
      421 acctggccga gggacagcta cgagcctata aggtaccaaa ctgatcggag agggcagcga
      481 ggtcagcgcg tgaatcttgc aggccgccga agggctagac caggaatttg ccgcgcgcca
      541 tgatttgctc gtcgtcgaac cagatatcga agtcgcggcc gacgcagtcg atgtgcgttt
      601 tggaaatcca ggtttgcccg gtatgctcgg cgtagggatg accgaaggcg atgtggatcc
      661 cggggatttt ttcgtcctgc aaaatattgc cgatgacgcg ggtgcaggcg gtattcgtgc
      721 cgacggcaaa ttctccgacg cgattgctgt tctcgtccgt gctggtgtag gcgcgaaatt
      781 cctcgagtaa ctcccggttg tcgcagacta acgaatgaat gcggttctcc ttcaccacaa
      841 tggtgagggg cgtcttctgg atgtcgccat acttttggca aaggtaatcg ccgaccacgc
      901 cgtcgaccac aaacgtgccg ttggtgttcc cgggtgaggt gaaaatctcg cccccaggca
      961 ggttgcccca tttgtcgggc gtgatgatgc cgctggtttt gatccacttc agcttcggcg
     1021 agagttcggc gacgaagtcg gtcccatgcg gagtccggca ggtgacgcgt ttggtgcggc
     1081 gggcctgctc gacgagaagc tggctgtgcg cgtcgatcgc cgcaaaatcc gcccgcatgc
     1141 cctcgagcat gatctggcgg ctgatgttca ccatgtggcc atggcgaatg cgatgccggt
     1201 tgacgacttc gctcatttgc atgcgggagc tgagttcgcc aaactggctt tgcgcgcaga
     1261 aaatggagac ctgcgacgtc gcgagatcgt cgaggatgac ctgtggcatg gtggtcagcg
     1321 gccgccgggc gcattcttcg aggacaaaga cggcatagac ggagccgacg agttcgactt
     1381 cactctggag cgcggcggca atctcccgcg tcgcctcgtc ggtaatgatg gtgatgcgtt
     1441 cgccgggttg gagtcggagg caatcgcgaa tcgcgttgcg cgccccgggg atcagctcgg
     1501 gatcgacctc aatcgcagcg gtaaagaaac ggttaggcat ggcgcggaat gttcaggatg
     1561 gcgcgaaaag ccacgcgagg caattcgcct tcaagcctgc acttcggtcg cggctcttcg
     1621 ttgcggcacg gtcaggcctt cgtaggtctc gttgacgatc aagtcgacca cgtcgtgcat
     1681 gttgtgctgg cccgcccagg cggcgatttg ccggtcggcg ccggtgcctt cccgcatgat
     1741 cttttcgacg tgcgcgatct ccgcgccgct cccgagttca tccacttccg tcgcgatgaa
     1801 atcgaggagt tcgccgagca atttccgagt gtcgacttcg gcttcgcggc cgaaatcgat
     1861 catcctgcca tcaagcccat agcgcgaggc gcgccagcga ttctcgtcga tcaggcgacg
     1921 gcgataaacg cggaaggttg tgttgtgata gagcaattta tgcagcttgg ccacgatcgc
     1981 ctggatgacg gcggccagcg cgagagtgtc gtcgacccgc gattgcgcgt cgcaaatccg
     2041 gatctccagc gtgtcgaaaa aaggatgcaa gcggatgtcc caccaaatct tcttcgcgtt
     2101 atcgacgcag ccggtcttga tgaggagctt gaggaaatcc tcatactccg aaaggctctc
     2161 aaaggcgtcg ggcgttccag tgcgcggaaa gcgttcgaag accttgaggc ggtaaccttt
     2221 caggcccgtg ttgcggccca cccagaaggg ggagttggtc gagagcgcat aaagatgggg
     2281 cagaaaatag cgcgcctgat tcatgacatg gatcgcgatc gcgcgatcgg gaacgccaac
     2341 atgcacgtgc agaccgaaaa tgaggttggc ccgcgccacc tgttgcatgt ccttcacgat
     2401 ctcggcgtag cgttcgccct cggtgatgtg ttgatcgaac caatgggaaa acgggtgggt
     2461 cccagcggaa gcaatcttca tcccgccctc gcccgccatc gccgccagct cgctccgcaa
     2521 ccgaaggacc tgccggcggg catcgtgaat atcaccgcag acatccgtgc cgagctcgac
     2581 aaccgactgg tgcatctcgg gtttgatctg ttcattcagc gtcaccttgc cttcgccgag
     2641 gatttgctgg atgtgcgagc gcagttcgcg cgtctctgga tcgatgatct ggaactcctc
     2701 ctcgatgccg agggtgaaaa cgtgatcggc ggcgctcatt cctcaggaac aaccgccaac
     2761 tctggttgcg gcgcaagccg gagagcgcgc cataaagagg gccgcgaccc ttttctccgc
     2821 agctgaaatt cactgcaacg cccacgtttg gcggagaaat tgcttatgac gacgcaactg
     2881 cccgaagatg caactctccc tgcacccccg tcaccacggt ttcaccctca ttgaaatcat
     2941 gatcgtcgta tcgctcatcg cgttgctcgc gatgatcgcc gtgcccagtt ttctccgggc
     3001 ccgccagcgc gcggaggccg tcgcggtttt gaatgaactc cgcctgctcg acgccgccat
     3061 cgcccagtac gcagtcgata ataacaaaac gaccggcgcg gagattacca tcgtggatct
     3121 gcaaccctac ttgaagaagg gcggagcgct ctataacacc ggccaggatg tcctcggcaa
     3181 cgatttcggc accttcgcgg ttgactccct cccactcgtc ccgaccgcga cgtggagggc
     3241 tctcgccgac gttgctgaca aggctttctg gtcgccttac tatcaggatc aagtagcacc
     3301 ggccgaccct tcgacgcgct agagcagtga gtcattaggt tatgagacga ccgcgtcgcc
     3361 ctctcaacct ggacatgcgg aagcatgtcc ctccggaagg gactcgccgc ggcgggccca
     3421 gaaacccaca gaacaatgac tcacttactg gagcgtaaag gcgtcattga tcacgcaggc
     3481 ctgctcgcgc ttatgcacgg cgagcgcacc gaccgccggg ctcgcgctgg cgttgcgccc
     3541 ggcgtagaga atttcccggc ctaacatcgc ccgcaggcgc ggctcgagat aggtccacgc
     3601 tcccatgttt tgcgattcct cctggcacca gacaactttc tgcgccctgc taaagggcgc
     3661 gaggacggcg cgcaacttct cctccgccag cggatagagc tgctccagcc taacgatggc
     3721 ggcgctggcg attttctccg cctcccggtg gttgaggaga tcgtagtaga ctttgccgga
     3781 gcagaggatg acacgctcga tcttctccgg cggcgccacc tccggcgcgc cgaggatttc
     3841 cgaaaaactt cctgccgtaa aatcctccgt gcgcgacgaa gcctgctcga agcgaagcag
     3901 gctcttcggc gtcatgatga tgagcggctt aatgaagtcg cgcttcatct ggcgacgcag
     3961 gacgtgaaaa tattgcgccg cagtggtgag gttgcagacc tgaatgttgt cctcggcgca
     4021 ggcctgcaga aagcgttcga ggcgcgcgct ggaatgctcc ggtccctgac cttcgtagcc
     4081 gtgcggcagg aggagaacga tgccgcttgg tcgttgccat ttcgattccg cggagacgat
     4141 gaactggtcg atgatggtct gcgcgccgtt cgcaaaatcg ccgaactgcg cttcccagag
     4201 gcagagcatg tttggataat cgagcgaata gccgtaatcg aaaccgagca cggccgcctc
     4261 cgaaagaagg ctgttgtaaa tgcagatccg cgcctggttc ggggcgagat ggaggagcgg
     4321 gagatacggc tggccactct tcgcatcgta aagaaaagag tggcgatggc tgaaggttcc
     4381 gcgccggcta tcctgccccg acaggcgcac cggcacgccc tcgagcagaa gcgaaccgaa
     4441 ggcgagcgcc tcggcaaatc cccagtcgta cggtccgccg gtttcatacg cttcgcgctg
     4501 ccggtcgatc gtgatccgtt tcactttcgg caggacgttg aaatcgtccg gcactctcgt
     4561 taggccgtcg acaatttgct tcagcagttc cgggctgatc gccgtcggtc tggatttgcc
     4621 ggagtatttt ggctgaaaga ccgcggtcga ctcgttgaaa cgatctctcc ggccctcgcg
     4681 cgatctctcg agcgccttca cttcttcgag ggcgccctcg aggcgatctt gcagatcttt
     4741 ttcgagctgg gccgcggcct ccgcgctcag cacgctggtc tcgatcaatt cccttttgta
     4801 aagctgcgcg accgacggcc ggttcgcgat cctggcgtag agatcgggct gggtgaagag
     4861 cggttcgtcg ccttcgttat gcccgtagcg gcgatagcaa tacatgtcga tgactgcgtc
     4921 gcgcccaaac tcctggcgaa aatcgagcgc cgtttcggtg acgaagcgga ctgcgatcgg
     4981 gtcgtcgccg ttcacgtgga agatcggcgc ctcgatcatc ttcgcaatgt cggtcgcgta
     5041 ggtcgaagag cgcgcgtctt ccggcagggt cgtgaaacca atctggttat tgaccacgac
     5101 atgcaccgtc ccgccggtgc tgtagccgtg gagttgcgac atgttgaggg tctccgcgac
     5161 gatcccctgg ccggcaaacg ccgcgtcacc gtgcacgagg agcggaagga ctttccggcg
     5221 atgctcggtg tcgccgcgaa tgcgctggcg cgcgcgcgct gtcccctcga ccaccggatc
     5281 gaccgcctcg aggtggctcg gattagccgc gaggcgaatc tcgatttccg cgccggaagc
     5341 gagctggcgg atggtgtgat agccgaggtg atatttcacg tcgccatcgc ccgccacgag
     5401 gtcgggaatg tagttcgtgc tgaattcggt aaagaggacc ttgagcgatt tttggagaaa
     5461 attggcgagg acattgagcc ggccgcgatg ggccatcccc atgcagattt cctcgatccc
     5521 agcgctcgga gagcgatgca ggatggtgtc gagaatgacc atgagcgact ccgcgccctg
     5581 aagcgaaaaa cgcttctggc cgacgtagcg ggtgtgcaaa aaaacttcga acgcttccga
     5641 ttcgaggagc agccggagga gcgcgatctc gacctgcgtc gaggcctgat gcttcgccgg
     5701 ccgcgtttcg agtcgatcgc gcacccaatt cctgatgcgt ggattttgga tgtgttgaaa
     5761 ctcggtgccg atggcgtcgg cgtagatctc atcgagcgtc gcgcgcatcg cgcgcagcgt
     5821 catcttcttg ttgtcgagga agaatttcga agcgacctgg aggtccaggt ctttctcgct
     5881 gaaaccgagc tcgcgcagcg tgaggagcgg attttccggc cgatgttcgg ccagcggatt
     5941 cacccgcgcg atggtgtgcc cgagggtccg gtaggcgtag accaatccat cgacccgggt
     6001 ctggagcggg ctctcgcctg gctcggcgcg cggcggcgcg gcgactgcag cgccgtttcg
     6061 ctgcggagta tttccaagct caaagccttc gaagaacgcc cgccaactgc tgtcgactga
     6121 ctccggatcc ttctcccaac ggcggtaatt gtcatcgatc aggtcaagat tgccacggat
     6181 cgcgaaggaa gggcgcatgg tcccagtacc ttgcaggcgt ttgcccgggc ggcaagttga
     6241 cactgccggg tcagcttcga tgttcgcctg caaaagccag gcccgcgagc atgacagcag
     6301 aggaagttta tgaatccgtc acgaacggcg gtacgagcga ttttgcagct gccgtggcga
     6361 ttttcgatcg cttcggtccg ggttcgtggt gtttgatcgg cggcctggcc gtgaactgtt
     6421 acgtcgagcc ggtctacacg gtcgatgccg atttcgtgat ggtcgctcgc gaaatgggca
     6481 aggtccaggc agccctgacc gccgcggggt ttgcactgca gacgtttccg cattccgtca
     6541 acgccagacg ttcggggagc aaactcagct tgcagttgac gaccgacgcg cgctatcagg
     6601 attttctcgg acgggcggag cggcgggaag ttctcggcct aagagtcccg gtggcgacac
     6661 tggacgatgt ggtccaaggc aaggtctggg cctggcagga cgaggaacgt cgcctaacga
     6721 aacgaaagaa ggacgagctc gatctgttgc ggctgggcga agcgtattcg catttgtccg
     6781 cggtgctgcc gccagagatc gttaggcaac tggaaacgaa atcgtgaaaa tcacgcgacg
     6841 tgaaacagca agttccggag ttctcggcgc cgcgctcggg ttcctttcgc tgctccgcct
     6901 ctcttccgcc gttttcgccc aggccgaccc tgcgtccgag ccctctgtgc tcgcggtcgc
     6961 gaaagtcctg cccgcggtgg tcaacatcaa caccgagcgg accgttcggc ggcaggtgcg
     7021 cgatcccttc gatgatttcg ccgcgcagtt tttcggcgct tatcgcaatc gtccgcgcga
     7081 gattcggcag cgattgcaga gcctggggac cggatttttg gtcgatccag cgggttacat
     7141 cgtgaccaac gaacacgtcg tcgagcgcgc ggcggacctc aagatcgagg tcatcacgaa
     7201 cgacaacaaa agcttcaaag cgcgctacat cacgggtgat gcgaaaaaag atctcgcttt
     7261 catcaagatc gacagcgacc agccgctccc tttcgttaac ctgcacgaca tctcgcccaa
     7321 tctgctcggc cagacggtca tcgtggtggg caatgcgctt ggctacggca gttcgatcag
     7381 ccgcggcgtc ctgagcggcg tgaagcgcga catcacgatc gataacgtcg attaccacaa
     7441 cctgctccag accgatgcgg cgatcaatcc gggcaacagt ggcgggccgt taatcgatct
     7501 ttccggacgc cttgtcggaa tcagctccgc gaaaatggcc tttactcccc aaggcatccc
     7561 gacccagggc ctcggtttcg ccatcccggc ggagatcgtt aggcaaaatg tcgagcagtt
     7621 caaaaaaatc gcggaaaagc agcccccgcc caaacccggc gccaccgaag agcagaccgg
     7681 ctcctccaat gcggatcgtc tcttcggatt gcagttgcag gatttgaccg acgacctcgc
     7741 cgccgcgctc ggtctcgcga gcggcaaagg cgttttgatc agcgcggtcg agccggacag
     7801 cccggccgac gaggcgggga tcgaacgggg gctcgtcctc tttcgcattg ggaaatacga
     7861 ggtgaattcg gtcaaggaag tggaaaagct gctggcccag gccgacagcg gctcgagcgt
     7921 cgatttcgcg ctcgggatca tcggccgcgg cgggcgcggg cggcgcattg aaacggtcaa
     7981 cctcgaagcg cgctaaaaat tttgccgaaa aatttcaaac acttcgcttc ctggggtagt
     8041 cagtaagact caccgccgca tgatcaaagt cgaaaatctc accaagcgct acgccgggca
     8101 gacggcgatc gatagcctca atttcgaggt cggaaaaggc gagatcatgg gtttcctcgg
     8161 gccaaacggg gccggcaaga gcacgacgat gcgcattctc tcgagcttca tgccaccgac
     8221 ctccggtcgc gcctccgtcg caggcttcga cattttcgag aagtccctcc aggctcgcgc
     8281 gcacctcggc tacatgccgg aaaacgtgcc cctctataac gatatgcgcg tgaccgaata
     8341 cctcgattat cgcgccgcgc tcaagggcgt cccacatcgc cgcatcaacg agcgcgtcgg
     8401 cgacgtgaag gagttgtgcg ggttgaaaga cgtcgagaaa aaattaattg gcgcgctttc
     8461 caagggttac cgacaacgag tcggcctggc cgacgctctc gtccacgagc ccgatctcct
     8521 gatcctcgac gagccgacga gcggcctgga tcccaatcaa attcgccagg tgcgcgagct
     8581 aatcaagaac ctggggcggc agcacacgat cctgctttcg acccatatcc tgcctgaagt
     8641 cgagatgacg tgcagccgcg taatcatcat caacaagggc cggattgaag cctgcgacac
     8701 cccggagaat ttgctcaacc agatgcggac ggcgaccggc gtgctgctcg aagcgcagac
     8761 cgggacggat aacggggtcg aggaactgaa gaaaatttcc ggggtgaagg atgtcaacct
     8821 aacgaccgaa ggcgactggc agattttttc cctgcgcgcc gaagccggcg ccgaagtccg
     8881 gccggaggtg ttccgcctgg cgagcgagcg gcgctggaag gtgcgcgagt tgtcgcaacg
     8941 caaggccacg ctggaagacg ttttcgtgga gattacgcat gcagatgaga tatgagccat
     9001 ttcgtcatcc cgagcaccgc cgagggatcc cgtcgcatct cgtgcagaca acgccacgga
     9061 cttccatgct ttccttgcgg ccgaacttaa tggtcacgcc acgggatccc tcggctgcgc
     9121 tcgggatgac gcattttgcc taacgagaca atgagaaaat tttttactct cctttcccgc
     9181 gaaatccgga gctatttcta ttcgccaatc gcctacatcg tcctcgtctt tttccttctt
     9241 gtcagcgggg tcgactttta cttccaggtc tcgttcatga accagcgggc gattccctac
     9301 accgtgcagg aagcgttctt taactccgtc ttcttctggt tcgctttcgt cctcatattc
     9361 ccgctcatta cgatgcggct ttttgccgag gaatttaaac tcggcacgat cgaaccactc
     9421 atgaccgccc cggtacgcga ctggcaggtc gtgctctcga aatattttgg cgcattcttc
     9481 ttttacatcg tcctctggat tccgaccggt ctctatttcg tcattttcca ggcgctcacc
     9541 catcagccgg cagccggctc gaccggcgcc tatctcggcg gctatctcat gctcctgtta
     9601 ctcgggatgt tctatctttc gatcggttgt ttcgcctccg tcctgacgaa gaaccagatc
     9661 gtggctgcga tcatttcctt cgccaccatc acactgcttt tctttctcgg gctggtttcc
     9721 ttcatcctcc tcgatatcag ttcggacacc cggcaactgc tcggctattt ctccgcgatt
     9781 gaacaaatgg ggacgctttc ccggggcgag atcgataccc ggccgatggt cctctacctc
     9841 agcatgacga tcgtgctcct gaccttcacc taccaggcgt tccagacgcg gaaatggaga
     9901 ctctaagcca tggccgacga gaccagttcg cctaagaagg cgatcaagat ccatcgtttc
     9961 cagatcgggt tgaacgtcat cctgcaggtg accatcatta tcgtgctcgc ggcgatggtg
    10021 aactatctcg gcttcgagca ctaccggcgg tgggatcttt cgcgcgacaa aaaatacgcg
    10081 ctctcggaca agacaaagaa ctttctcgac agcatcaagg gcaaggtgcg aatcaccgtg
    10141 ttttttggct cgcaagaccc gatcaccagt gacgtgcaga atctgctgac cgaataccag
    10201 tatgcctcgc atgggaagat cgacgtcgaa tacgtcgacc cggaacgcaa cctgacgaga
    10261 gcaaaggaac tcttcgataa atataaagtg gtgaccgacg agagcgtgct gatcgtcgat
    10321 taccagggtc ggagcaaaac cgttaaagca tcggaaatgg ccgacatgga tcagggtaat
    10381 ccgttggccg gcgaacagcc gaaagtcgcc gctttcaagg gcgaacaggc gatcaccgcc
    10441 gccctcattg acatcgtgga ggggaagaaa aacatcgtcg gctgggtgac cggtcacaag
    10501 gagccgcccc tcagcggaga gagcccggtc tcggtcctga agacgtttat cgagaacgag
    10561 aacatcacct tgcgggagct gaacctgttc gaagtgccgg ccatccctgc tgatttgaaa
    10621 gcgattgtga tcgccgggcc gcagtacgat ttctccgatc gcgaaatgaa aatgctgcgc
    10681 gaattctgga acaaggatgg gcggattttg cttctcctcg atcccgcggc caagacgcca
    10741 aagctgctcg cctttctcgg cgagctcggc gtcaaagagg atgacgatcg cctgatggcg
    10801 atggtcaaga ccggcatcga ggaagtcgca cgggtgcggg acgtggtcgc tcattttctc
    10861 agcgacaatc cggtcacaaa acggctggcc gatgtacacg ccccgttctt cggctcgacc
    10921 agttcgttaa ccctcgagtc acagcaggtc gcggcagcca atattcatct cgcaccgctg
    10981 gcccaggcag agaagggcta ttggggcgag acggattatt attcgaatga cgaggcgctg
    11041 ctccaattcg atccggcgaa agacaagggc gagccgctta cgatcgcggc ttccgtcgag
    11101 aagggcggga gtgccgatga gcgcgtccag atcaattccg cgcgcctcgt ggtggtggcg
    11161 aattccactt tcattcagga caacgccctc acccaggacc agcagggcct ggacttcgtt
    11221 tccgccagcg tcaactggct cctcagccgc gaacaaatga tcggcatcgc gccgaaaatt
    11281 ccgcagacgc tcacctttgt cctcgatgaa aaggcgctgg ccagcctgcg ctggatcatc
    11341 ctggtgctga tgcccctggt gccggcgatt ttgggatttg cggtgtggtg gcgacggcgc
    11401 gcctaacgac tgcccggcat gaaaaccagg acgacgctcc ttctcctcat cctcaccgtc
    11461 gggctcggcc tttggatcaa atatttcgaa agcaagaaac cgaacacggc cgaggcccag
    11521 cgcgaggccg gcaatgtcgt gaacttcgat cgcgagaagc tcgaaggcat cgtgatccag
    11581 aacggggatg accgcatcga gctccgccgg cagaatggaa aatggcggct gaccgagccg
    11641 gtgaaagacc aggcagacgg agcggccgtc gataatttga tcggcgacat cgatacctgg
    11701 cggagggacg gcaccgtgcc cgccaaagag gtgaccgcgg ataaggggcg gatgaatgaa
    11761 ttcggtctcg tgcagccgaa gttgcggata cggttaatcg ggccaaacgc gccgcccgag
    11821 attctttttg gcaaggacgc cgcactcgag ggaaaaatgt atgtgcgcct cgctgattcg
    11881 aaggatgtta tcatcgccgc tcagtcggtg cgcacggaca tctcgaaaaa gccggatgat
    11941 ttccgcgatc ggaaattgac cgacctgacg accgcacagg tcacccgtac ggttttgaaa
    12001 agcccggccg gcgaaatcga actggcgaaa aagaacgagc actgggaaat cgtgaagccg
    12061 ctggaggcgc gcggcgacga ccagaaggtc gacgacctgc tggcgcaggt gacgaacgcg
    12121 cgcatccagg aattcgtggc cgatgaccgg ggcgacctgc atacctacgg gctttccgag
    12181 ccgcgcggtt cgatcaccat ctacggcgcg gacgacaagt ccgccagtcg gacgggctcg
    12241 tcccgcggcg aggaaggccg caccctgcag ttgggcgccg tcgcggaaaa gatgaaggac
    12301 gctatttacg cgcggttcct gccgcggaat gcggtttatg ccctccccaa aaagactgaa
    12361 gaaattctaa ccgttcggcc taacgattta cgggatcgtc atctcgtgag gctcgatacg
    12421 aacaatctcg accgaatcaa cctcgaagcg accggacagc cgaagatcgt gctcgcccgc
    12481 aaagatcaaa actggacgat ctcgagccgc aacaatcagc cggccaacgg cgacgaagtg
    12541 cgccggctga tcgacaccct gaacgatcag caggtgacca ggtttgtcgc cgacacggcg
    12601 tccgagctgc cgaaatacgg gctcgaccac ccgcaactcc gattgacctt cagctccttc
    12661 gcctcggaaa atacggcgga gacggcggcg ggggagcatc cgttcctgac cctgtcgttc
    12721 ggcaaggttg agggggacga cgtctacgcg cgggtcggcg aagagccttt tattgttgcg
    12781 gttaatcacg agcttctcgg gcaggtctgg atcgagccga cgcaatggca ggaactgtcc
    12841 gtcttcaagt tcgcgcctaa cgagattcat cgcatctccc gcatgaccaa ccacgaagaa
    12901 tcgctcgtcc ggagcggtcc gcagagttgg caatggagca aaggcgaagg cgcgatggat
    12961 aacgccgacc ttcagtcgct cctcaacacg ctcgcctcct tgcgcgccgt gcgctgggtg
    13021 ggcgcgacaa acccggcgca gggtttcgac aagccgacgt tggtcctgat cttcaccacg
    13081 tcgcccgacg acaaggcaac gcacaaactg accatcggcc atcgcaccga cgatgcgatg
    13141 tggttcgcga aggtcgacgg acgggacggg actttcgtcg tgagcgaccc ggattttggc
    13201 gcatttaaat tgcccctgac gaaggcgacc gctgcctcgc ccacgcccgt ggcaacaccg
    13261 attggtcgcg tcccaagttc ggccaccgca gcgccatcac ccggtgagta atcaggccga
    13321 cgcgcagtag atcgtgacta tgccacccga gagcggctgg gcattggcgg cgcggaagcc
    13381 actcgccctc atcaattcac acatcgccgg gccgctcgga aacttctcga tcgacgcgcc
    13441 cagataatcg taggcctgtt tttcgcccgt cacgagcccg gcgagccacg gtaaacagcg
    13501 gtgcaggtaa aaccgatata tgggccgcaa gaatcctcgc ggaagcgaaa aatcgaggac
    13561 caacaaatgt cctcccggca ccagcacacg cgccatctcg gccagcgccg ctctccagtc
    13621 cgccatgttg cgaagcccga acgcgaccgt cacgacctcg aagcgtgccg cggggaacgg
    13681 aaggtgaagc gcgtccgcca cgatggttcg ggtcaggccc ttggcacgcg ccgtcgccag
    13741 catttccgcg caaaaatcgg ccccagtgat ttccgcctgc ggcaactttt gagcgatcgt
    13801 tagggcgaga tccccactgc ccgtcgcgag atcaagcacc cgccgcggat tccagccgcg
    13861 cacgatctcg gacgcgcgat cgcgccacca aaaatcgagg ccgccactga gcaggtgatt
    13921 cgccaggtcg tagcggcgcg cgacgcgccc aaacatcgca cggaccttct ccggatcacg
    13981 atcgacgagg ttcgaaatcg gaagatggtt cacgttccct gttctagcgg cggttcgcgg
    14041 cgatcgccaa tttttctgct ccggagcgac gcttccgcac gtccccacgg cacttcccgg
    14101 ggcgaaagat cgaagacgtg cggcagcacg tcgctccggg aagctaatcg aagggcggct
    14161 cgctcttgtc ctcgtcgcgc gggaccagtt tgttcttcgc gatgatgcgc gcgcaggtgt
    14221 tccagcggag caacgcatcg tcgttccccg gcggccggat cgcctcggct ttttcgtaaa
    14281 actccatcgc ttcgcgcaag acgtgatagg cgtagtgatg cgcgccaggc gtgccttgcg
    14341 cgagcttcgc cttggcccgc cgctcggcca gaatgcccat gtagtaggcg cgttcgtaat
    14401 cgcctttcac ccgggccagc aattggccgg cctgcgcgtc gctcacgccg taacctttgc
    14461 tgaagcgatc ggtcagcgcg aggaggagcg taatgatcgc ctcctgattt tccggctccg
    14521 cgtggagaac atcgaggcag atactttccg cctcggccgg ttcgttgagc aggcgatagc
    14581 gctccgcctt ttccagggcg gcggggatcg cttcccgcga aaggtgcttc aggtcagaca
    14641 tggtcgggtt cgtgaggcga ggaagaagat acgcgctgac tccggcgccg cacaatcttc
    14701 cagagccagc ctaacggatc ataataatcg tccaccacgc gttccttcaa gggaatgatc
    14761 gcgttgtcgg tgatgtggat gctctcggga caaaccttgg tgcagcactt ggtgatattg
    14821 cagtagccga tcccctgcgt cgcgcgcaat tcctcgagcc gatcctccgt gtcgagcggg
    14881 tgcatctcga gcgccgcaac gtaaatcaaa aaacgcggcc cgatgaactc gtcgtacttg
    14941 cgatgatcgc gcaacacatg gcagacatcc tggcaaagaa aacactcgat gcatttccgg
    15001 aattcctgga cgcggtcgat ctcctcctgc tgcattcgcc aggtcccgtc cggcgcatcg
    15061 ggcgggcgcg gcttgaactt cctgatcttt ttcttcaccc gaaaattcca ggagacatcc
    15121 gtgatcaaat ccttaagcaa aggaaacgcc ttgatcggct cgacggtgac cggcttgtcc
    15181 agcggcaatt cgcttaaccg ggtcatgcac atgaggcgag gcatcccgtt gacctcggct
    15241 gaacaggaac cgcacttgcc tgctttgcaa ttccagcggc aggcgaggtc gttcgcttcc
    15301 gtcgcctgaa tgtgatggat ggcgtcgagc acgaccgtgc cttcggagac gccagtttcg
    15361 taatcgcgaa actccccgcc cgcggcgttt ccccgccaga ttctgaaggt ggcggttttc
    15421 atatgagcgc gggttgcact ggcccgggta gcgcacgcgt ctcacgtgct ggttgcggcg
    15481 ttccgccgca atgaactttg tcagccaact cttttcgaaa tgaacgtcgc caaaaggcaa
    15541 tcgccagcgg aatgaaaagt tcgtcgcggt gaaacatcgc gaccggcacg cgagacgcgt
    15601 gcgctaccca ggcaagatta cctgcgttca tcccatctcc tcgatcacgc gtttcaattc
    15661 ctcccgcatt tcgggaatcg gatcgcgccg aatccgcatc ccgccgtctt cgcctttcca
    15721 gacaatcgtg ttcacgtttc caaactccgc gcttttctcg gggtaatcct cgcgaaattg
    15781 cgcgccgcgg ctttccttcc gttcgagggc ggcgcgcgtg atggcttcgg acaccgtgag
    15841 caggttctgc aggtcgagcg ccgtatgcca gccgggattg aagtcgcgat ggccggtcac
    15901 gccgacctcc ctggctcgtt cccacaacac ttttaaatgc tcgagagcgg actgcatttc
    15961 gctttcctcc cgcacaatcc cgacattgtg ctgcatcatc tcctgcaaat cccgctgcac
    16021 ggtgtaggga ttgtcctggt cgcgattcgc gaatggctga agcgcctcgg cctccagctt
    16081 tttcacgatc gcctcgtcga tctcgcctaa cgactgaccc tgggcgaatt tcgcggcgaa
    16141 ctcgccggcc cgctggccaa agaccaaaag atcggagagc gaattgccgc cgagccggtt
    16201 cgcgccgttg atccctgcgg cgcattcgcc ggccgcaaag agccccggca cactggacat
    16261 ctgcgtgtcg ccatcgaccc gcacaccgcc catgatgtag tgcgtggtcg gcccgacctc
    16321 catcggctcg cgcgtgatgt ccacgtcgcc cagttgtttg aactgatgat acatgctcgg
    16381 cagcttgcgc ttaatgtgtt cctccgcgtt cggcagccgt ttcttgatcc aggaaatgtc
    16441 gagatagacc cccccatgcg gcgttccgcg tccttccttg atctctcgca caatgcagcg
    16501 ggccacgtga tcgcgggtca gcaattccgg cgggcggcgc gcctctttgt cgccttgcgt
    16561 gtagcgccag ccttcttctt cgttgtcggc cgtctgcgct ttgtagttct ccggaatgaa
    16621 gtcgaacatg aaacgctggc catcgttgtt ggtcaaaatt ccgccttcgc cgcgcacgcc
    16681 ttcggtcacg agcatcccgc gcacactcgg cggccagacc atcccggtgg gatgaaactg
    16741 gatgtattcc atgtcgatga gctccgcacc tgcgtgatag gcgagggcgt ggccatcacc
    16801 ggtgcagtcc caactgttgc tcgtgatttt gtatgctttg ccgatccccc cggtagcgag
    16861 gatgatcgcg ttcgctcgaa aaatccggaa ccggccgcgc tcgcgatcgt agccgaacgc
    16921 gccgctgata cgtgccccgt ccttcagcag agcgaggaca caaacctcca tgtggacatc
    16981 gatcccctga tgaatcccgt ggtcctgcag ggtgcgaatc atctccaggc cggtccgatc
    17041 cccgacatgc gcgaggcgcg gatacttatg gccgccaaaa tttctttgca gaatccggcc
    17101 atccttggtg cgatcgaaaa cggcgcccca ggcctcgagt tcgcgcaccc gctcgggcgc
    17161 ctcccgggca tgcaactccg ccatccgcca attgttcacg tactgaccgc cgcgcatcgt
    17221 gtcggcgaaa tgcaccttcc agttatcgcg gtcatccacg ttggcgagcg cggccgcgat
    17281 cccgccctcg gccatcacgg tgtgcgcttt cccgagcaga cacttcgaaa ccatgccgac
    17341 cgagacgccg gccgcggaag cttcaattgc cgcgcgcaaa ccggccccgc ccgtgccgat
    17401 gaccagaacg tcgtgcgtga aagtttcgta gtcgggcatg cggcgcgaag cttaaaaaaa
    17461 tcggagatcg tgccacacgc ccacggcgca aagtcggacg tagacgtcgg agaacgcgac
    17521 ccagaacaaa ctcatccagg cccagagcat gtgccgtcga ttcaaacaac tgacgcaggc
    17581 ataaccgcga tagcacgccg gtgctttcga aaactgatcc aggaatccgc cgacgagatg
    17641 acgcagggag tggcagccaa aagtgtaacc tccgagcaaa accacattca ccgccagcac
    17701 cagcgtgccg acacccattc cgaaggaagt tcggccggtc gcgggatcga tgaaccacag
    17761 cgcttcccaa acatcgtgtg cgagcacgag gatgaaaagg agcgcgaggt agagaaaata
    17821 acggtgcaca ttctgcatga tgagcgggaa ggagcgctca ccgagatacg tgctgcgcgg
    17881 ctcgccgacg gtgcaggccg gcggatccgc ccagaaagcc ttgtagtagg cgccgcggta
    17941 gtaataacag gtgagacgaa aaccacccgg cgcccagagc acaagaagcg agggagaaaa
    18001 aatgagccag gcaggccacc aaactggttt cgcgccaaac cagctgtgcg gcgaggcgcc
    18061 aaaaatctcc ggcgaataaa atggggaaag atagggcccg aaataataat tgcggccctg
    18121 cagcgccgcc caggtcgagt aaaccacgaa cgctcccagg ccaagaaaaa cgacgagcgg
    18181 ctgcgcccac cagatatcgg cccgcatcgt ctggccaaac gagcggcggg aaagagttaa
    18241 ttctgcttgt gtcatcgcgg cgaactctcg gttcgctcgg ttagataacg acggtgcgag
    18301 gggttgacaa gaatttcccc gatcctcctg gtcttcaatg cttccccgca gcgggtgcga
    18361 gcgaggccac aatctggtcg aagcgcggat cgccccgcac cgggtcccaa aatggatgca
    18421 ggcgaagctg cccgtaactc acgtcgctcg ggatcgccgc ggcgcgtttc aactgctcga
    18481 tggcctggtc ggtctcgccc gtccatgcat aagtcacggc cagatattcg atcgcgagcg
    18541 cgccattgat cgaatctttg tcgagcggca gcagatcgac cgcgcgccgg ccttcccgaa
    18601 tcgcgtcgtc cttgcgccca agtgcggcgt cgatcatggc cagcacacaa agcgactgac
    18661 cctgatccgg cgcctcccgt aaacttcgct cgacctcttc gcgggcggcg acgaaagccg
    18721 cgcgttcccc ggtcgcatcg ccccgcacgc gcgcaaccag agcccgacac cacggacgcg
    18781 aaaaaagaac gccttcgttg ttataacctt cctccctcat cgcggccacg gcacgatccg
    18841 cggtgtcggg ttcgcgttcg caaagggaca ggaaaagcca ttcgtcggcc agaccggcgg
    18901 cggcttccgg gttggccgtc acgatcttct cgatggtgga gtgcaggggc ctgggatcgg
    18961 cgcgctgatg cagatcgatc ccggcccggg ctacccgcag actcatatcg cccggccgaa
    19021 ttttgagcgc gcggtcgagg agaccggcgg catcggcata gcgccgcaac aattgataac
    19081 taacggcgag ctgctgcagg atgtaggtgt cgcgcgggtc gagatcgagg gcatgcgcaa
    19141 gattccacgt cgaggcatcc caccgaccct gccggcgatc gataaacccg gccagttcga
    19201 aaatcagcgg ttcgttaggc agagaaacgc gggcgatttc gagctcttgc cgcgcctcgt
    19261 cgtaggccag aaacccgcag taaagatgct ccgccaaggc gagatgcgtt tcgccggcat
    19321 cgggccgcaa gcgctgcgcg gccttgatcg cggcatcggc cagggcgagg cgcgccggcg
    19381 tgtgatcggg tccggtgaga taaatctggt cgtgcgcccg ggcgagtcga gaatatgcga
    19441 ggaaataatt cggatcacgg gcgaccgcct tttccagcag gtccacggcc ttaagaagat
    19501 tttcggtccc ttgcgcgttg aaaagcgggc ctgacatcag ggcattcgcg cggaggtaaa
    19561 gatcgtgcgc gaccgggtcg gaggtcggtt gcacttccat cgcggccttt tcctgcggcg
    19621 agagtttgct ccgcaactgc gcgacgatgg tttccgcaag gtcgctctcg agagcaaaca
    19681 cgttggccag gtcgcggtcg taatgttccg cccaaagatg tgcgtcggtc cgcgcgtcga
    19741 ttaattgggc gctgacccgc acgcgattgc cgacacgctg caccgttccc tcgaccacgt
    19801 gcgccgcgcc caattcgcgg ccgatatcgc gcagattgcg ttccgcaccg ctgcgatact
    19861 gcatggtgga agtccggctg atcaccttga gatcggaaac cttcgccagg tcggtgagaa
    19921 tttcgtcgtg cacgccctcg gcgaagtgcg cgttctcgtc atcgtcggag agattgtcga
    19981 agggaagtac ggccacgctt ttctccggga tcactgagga ccgatcagtg tccggcggtt
    20041 tcctccaggg atgccacacc gcgtaaccgg cgaccgcaac gcccgccacc aacaggcctg
    20101 tcacgccgag aaccaaaagc cggcggtaat ccgtcgcctg acccgcgcca tcgtcggttg
    20161 ttcgccgcaa gccgccatga ctcacttcga aaacccagcc caggacgagc gcgaccggaa
    20221 agcccagcgc gaccaggacg agaaaggcct gcatcgtcca atccggggcg tggaaagccg
    20281 gaaacaccgt cccaaccagc tgaatggtgg ccgaagcgcc gattacatag gccagcgcca
    20341 cgcgataaac gcgtcgccgc ttgagctcct gaaagaaagt cctcatcccg actcttctga
    20401 ctcagtttcc tataccggca gaggcggaat atcgctcgtt ttcttccgaa cccgaaacgg
    20461 gttttggaat tggcagatca ggtccggccc gaaagcggcc aggttgagca cgatcatgat
    20521 gagggcgaag agataaagac ccatcgtgag gccaatcgag atgtgcatgc ccaggatccc
    20581 ggtcaaccaa accgcccgcg tccttctcat ccagatgaac accggataac cggtctccag
    20641 caggcaaacc agaatcccga tagcaggcag caggtattcg aagcgcatga gcagtcccgg
    20701 tggcacgaga tcaaacggcg gccgggtcag tgcgcgccag acgctgtttc cattccacca
    20761 atcggcgccg aggcttttgg agatgccgct gaaaaaatag atcacacaga ggtggagctg
    20821 caaaacgcgg cgatgaaagc ccaaccgctc cggatttatc ctccgcctct ttcgaagccg
    20881 cgaatcgagc gaccatgggt cgggcagggg ggcgatcatc aaataaaaca aaccgatcgt
    20941 cgtaaagttg tcgactccgt aggcgaagaa agccccgctt ttaacgctgc agagatacaa
    21001 aaaccagctc acgcctgccg ctaatcggga gctccatcct gtgattaata aaagcccact
    21061 cacgaccaaa ctcacccaga cgagcgagag ggtagtggcc tccgtgagac caacgtgctt
    21121 ggcgacaaaa accagccatc ccagcctggg agtgtagaag ttttcgccgg agaaagccgc
    21181 ttcgaccagc gcccgagtga ttaagccgta gcctctcgcg ccgtaaagat cactccaatc
    21241 tgcgcgcaat gatactgcat agaggatgat ctgaaacccg agaccaacac gaagcaagct
    21301 aagccagcaa ccggagtcag gtgggaagag aaacgtccca agccattgcc attttctggc
    21361 catctcagtt taaggcagaa ccggagggcg agtcttagga tgaaatcgga agtcgtaagc
    21421 aaacaacgcc cggtaagaaa ttctcttccc cgctcgatac tcatcggcat tgacgaggtt
    21481 tgccactccc aagaccgctc gaatcaacgt cgcgtcggga tgttcccggc ggacagactc
    21541 tgcgaggctt ctgagcaatg cttcgcggag gggcagatag tgaattacct ggaggtaatc
    21601 gagcagggct gagacccggc cacccgccgc ggatccgccc accacgggaa ggtcgtattc
    21661 cactcggccg tcaggataat gcagctcgaa gaccagtttg cagttgccgg gaataaccgg
    21721 agcgaaataa ctgtaaccaa cctcgattcc actgcagttt gcat
//
LOCUS       DATMNS010000253         9942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3082248, whole genome shotgun sequence.
ACCESSION   DATMNS010000253 DATMNS010000000
VERSION     DATMNS010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3082248"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..824
                     /locus_tag="VNW28_08905"
     CDS             396..824
                     /locus_tag="VNW28_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXA10088.1"
                     /translation="MAEHSGKDVLSSDVEIKGSIKFQKELLIDGKVEGEINSDGVLTI
                     GENADIHGEIKTKSITVYGKVHGNITVGERCELKSRCTLQGDLKAARLVIEEGATFIG
                     KSEVTSGKSVAGSNSAGSRPEIVRNDESPAPSKAAFASRS"
     gene            847..1695
                     /locus_tag="VNW28_08910"
     CDS             847..1695
                     /locus_tag="VNW28_08910"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXA10089.1"
                     /translation="MAKSPAKLSVECPHCGFKQMEYAAAKNTLCRQCGKHFVVVTQRV
                     EPKPNLELGGVVAAQSLLRRFEGLWKRPRINSVACFDCGAAQELNSAATSTICPSCSV
                     HMDLRDYKINAVFSRAIRTHGEVHVTSSGDLSSSSVICRSALIEGKLRGSLHCLEKVE
                     FRAPAKVQGKLTAPLVVIGKKADVQFFRQLQVGSIEIRGRMSGEIVAQTLVTIRSTGS
                     LDGNVVARSINVEKGGTFSGLLVIGQKTWEQAEMLLPVVEGTKPRTPGNSRRPPPHPF
                     GLPATS"
     gene            1706..2128
                     /locus_tag="VNW28_08915"
     CDS             1706..2128
                     /locus_tag="VNW28_08915"
                     /inference="COORDINATES: protein motif:HMM:NF028108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5069 domain-containing protein"
                     /protein_id="HXA10090.1"
                     /translation="MQKLLPRSPYERLGGYVHLPRLIDKARLHRQGLLDGYNYKTVGF
                     DKHLLAFLKIDPDAFEKTANELDDDAAILDWVRRTGVQHSPAEIAQWNQAMIARHPDT
                     AVKNARFLHFLKEAGGEGRKDIRTYFDLIEFDEGRLKD"
     gene            complement(2210..2962)
                     /locus_tag="VNW28_08920"
     CDS             complement(2210..2962)
                     /locus_tag="VNW28_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXA10091.1"
                     /translation="MNVLILAAGYATRLYPLTENKAKPLLEVAGKPMIEWVLDNLAPI
                     PDLETIYVVTNDKFAQDFQAWADRYALAQPKLTFKIVNDGSRSDADKLGAIGDIRLVI
                     DREELTSAPLIVVAGDNLFSEPLADFAARARGSAATLATYDVGDLEAIKKYASIQTDA
                     AGIITHFEEKPEKPESTLTGIALYYFSPETLELFTTYIAAGNNPDQPGRFIQWLYPRR
                     PVKTYQIKGTWYDIGSKETLEEANQIFAQFAK"
     gene            complement(3032..5779)
                     /gene="glnD"
                     /locus_tag="VNW28_08925"
     CDS             complement(3032..5779)
                     /gene="glnD"
                     /locus_tag="VNW28_08925"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HXA10092.1"
                     /translation="MSDHQDKVLQHAERELAPARDAAPPHLLPLYQKFLKIENHRLRL
                     RHQAGGGGREVCASRASLIDVLLRHLFAAASASAEEGNGAKVVPVSLLALGGYGRGEL
                     NPGSDIDVMFLHPHTGDEVPASLAHTVEQVLYFLWDIGLKVGHSTRSVREAVAQANRE
                     MLTKTAMLEARHLAGDAALFQRFRTRFRADCVQGHEKEYIAGRMADQTERHQKFANNI
                     YLQEPNVKRGCGGLRDYQNLLWITYFKEGALTTTHLVGKDWLSAGDGRRIDLAYDFLL
                     RVRTQLHYTNERTTDVLHLSLQPEIARRLGYAQKTPVARSEAFMKDYYAHTRNIFRLT
                     ERLTERFATGTATTTRRLFFPLLPRRKPREEHFENFFSRGGQLHVSDPELFTRQPEQL
                     LKAFEHVQERKLELSAELTDLLSRRLGLVTREFRYGKAPREIFARILRRKGEVARILR
                     MMHEVDFLGRYLPEFGPLTCLVQHEYFHRYTADEHTLVCLEKLDALRDTENPKLQPYR
                     KLFEAMADPYVLYLALLLHDTGKGVGARPHSEASALFAQGVAARLQLSSEQRRSLVLL
                     VDHHVTLSTIAQQRNVDDPVTIIEFANIVKSKANLDALMLLTLADGQGTTDTWSDWKE
                     SLVWHLYHATARYLQDQEGFIAQGKIERDRLRNAVADQLAPDFADEIEAHFDYMPDNY
                     FRTFRAEEIVAHLALFRTLWRRIYLEDEPSFAPALRWEALPEQGHSVVSICTWDRQHL
                     LASIAGALAVASLNILSADIFIRGDNLVLDVFRVRNEKLGPVTDAREIVTVESTLRTA
                     LQTERFNFAPLLAGVRETAVRAPELDFPTRITIGNESHPSCTLIQVETPDRLGLLYDL
                     VACLARNNVYITLSRISTEKGAAIDTFYVTDATTRGKITETKRIEQVQRQLHEATLAP
                     A"
     gene            complement(5901..7298)
                     /locus_tag="VNW28_08930"
     CDS             complement(5901..7298)
                     /locus_tag="VNW28_08930"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXA10093.1"
                     /translation="MTTENRRILIIDDERPILMTLEALLGRHGYQAEMAANASTGMRL
                     LQSTAPALVLLDLQLPDADGLQMLEQIKADSPDTQVIILSAHDSLNNAIESIKRGAFH
                     FISKPYAPEELLSLVEKALEKQSLLRETIELRATTQRLKKRLAQAETRLAPVFKSKVM
                     QEIDELISAMAPSEANVLITGESGVGKEVIANVIHGRSRRAEKAMVKLNCAAFPQAMI
                     EGELFGYMKGAFTGATSDFRGMIAEAEGSTLFLDEISEMPADLQTRFLRVLQEREFRP
                     LGSTKTLRADFRVVAATNRPVHQALAENRLRSDLYYRLNTFQIELPPLRERREDIPPL
                     VASFVKRFALQLGKAEPAISPEAFQKLLEYGWPGNVRELQNAIEYAVVLARQDQITVK
                     ELPAEVRLPVVLQKTERAAQQAQSPARSLNDMERNAILDALAQCHGNKKKAAELLGIQ
                     RPTLYNKMKRYAIQL"
     gene            complement(7329..7976)
                     /locus_tag="VNW28_08935"
     CDS             complement(7329..7976)
                     /locus_tag="VNW28_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10094.1"
                     /translation="MTKFIGQLNHDLRNYLNAVELHAAFLGEIAEGPELKNEIKRLRE
                     MTGELGAHLHRLAHSLAAIEPNLMRYPAVEFIEDLRAKLALDQPERSATIEWQISLGG
                     EALEIDPQLLQEAFVELLTNAGTHDRAEGALVFAAGRSDQTVEFTLCEPKTKFDGTTE
                     NWGGRPFRQMRNGHYGLGLFRARSIFEAHHGNLRAQFDPAGSVLRTTVSLPLLVS"
     gene            complement(8076..9647)
                     /locus_tag="VNW28_08940"
     CDS             complement(8076..9647)
                     /locus_tag="VNW28_08940"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HXA10095.1"
                     /translation="MPANSLWQRFQEYYLRYDDLGFSLDISRMRFPDGFLEKMQPRVE
                     QAFAAMRALEDGGIANPDEGRMVGHYWLRNPALSPNEQLRADIVETNARIKQFAGAVH
                     GGQVANERGERFTHVLLVGIGGSALGPQFIAEALGATRDPMDIFFFDNTDPDGFDRVL
                     EKIGTGLAQTLAVVISKSGGTKETRNGMLEAAAAFTKAGLQFPKHAVAVTGVGSELDK
                     VAEKAGWLARFPMHDWVGGRTSVMSAVGLVPAALEGFAIDDFLKGAAAMDAHTRQPER
                     ATNAAMLLALMWFYAGDGRGQKDMVILPYKDRLALFSKYLQQLVMESLGKEKDLAGQV
                     VHQGIAVYGNKGSTDQHAYVQQLRDGVPNFFATFIEARQDRAVGRFEVEEGVTSGDYL
                     QGFLRGTRAALYENGRDSITLSIPQVDAFQVGALIALYERAVGFYGSLVNVNAYHQPG
                     VEAGKKAATRILQLQPKVRAQLSATGITADGIAQAIGADPEDVYHVLRHLAANDSSLQ
                     AGGEGPGSEKFSRIN"
     gene            complement(9739..>9942)
                     /locus_tag="VNW28_08945"
     CDS             complement(9739..>9942)
                     /locus_tag="VNW28_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HXA10096.1"
                     /translation="KGGTEDWRKVLKEATGEDLSTRAMVDYFQPLMAWLEEQNKGRKI
                     GWDEDKQSDHEDKEREEGRKEKD"
BASE COUNT         1902 a         3005 c         3043 g         1992 t
ORIGIN      
        1 cttcgtcgga tgacgcgcgc aaaattctcc gccggcatga ggtgcagcgg ttcctggctg
       61 gcgttccggt accggctgaa gcgttctgct tcacgctgaa tcggatgccg tcgcaagcgc
      121 ctagatttct gccacttgcc aggtaaaatg gggcctgcga ggcgtctcaa atgcgaaatt
      181 tacgttaaaa atcgttgcct ttgtctttcg taaacgttga cattaacaat tccgcctcct
      241 aataatgctg cgtgttaaaa aaatttatcc cacagaagcc cgcgccgccg gccgagatct
      301 ctttgcccgg ctcgacttat gctgaggaca atcactttcc ttctcatcga gaagaagcac
      361 cgaaatcacc aaccatgacc gagcaccaaa aatatatggc cgaacattct ggcaaagacg
      421 tcctctcgag tgatgtcgag atcaaaggaa gcatcaaatt tcaaaaggag ttgctgatcg
      481 acggcaaagt cgaaggggaa atcaattccg atggcgtgtt gacgatcggc gagaacgccg
      541 acattcacgg cgagatcaag accaagtcga tcaccgtcta tggaaaagtg cacggcaaca
      601 tcacggtcgg cgaacgctgc gaattgaaat cgcgctgcac gctccagggc gacctgaaag
      661 cggcccgcct ggtgatcgag gaaggcgcca cttttatcgg caaatccgaa gtgaccagcg
      721 ggaagtcggt ggccggctcc aacagcgctg gaagccgtcc cgagatcgtg cgtaatgatg
      781 agtcgccggc accctcgaaa gcggccttcg cgagtcgttc ctaacgagta gccacgcggt
      841 aagtctgtgg ccaagtcgcc tgccaagctc agcgtggaat gtccacactg cggcttcaag
      901 cagatggaat atgccgcagc gaagaacacg ctttgccggc aatgcggcaa acatttcgtc
      961 gtcgtcaccc agcgggttga gccgaaaccg aacctcgagc tcggcggggt cgtcgccgca
     1021 caatccttgc tccggcgttt cgagggcctc tggaaacggc cacgcatcaa ctccgtcgcg
     1081 tgtttcgatt gcggcgcggc gcaggaactg aactccgccg cgacctccac gatttgtccc
     1141 tcgtgcagcg ttcacatgga cctgcgcgat tacaaaatta acgctgtctt cagtcgggcc
     1201 attcgcaccc acggcgaggt gcacgttacc tcctcgggag accttagcag cagcagtgtc
     1261 atctgccgct cggccctcat cgagggcaaa ctgcgcggca gtctgcactg cctcgaaaag
     1321 gtggaatttc gggcgccggc caaggtccag ggcaaattga ccgcgccact agtcgtgatc
     1381 gggaaaaaag cggacgtcca attttttcgc caattgcagg tcggatcgat cgagattcgc
     1441 gggcgaatgt ccggtgagat cgtcgcccaa acgctggtca cgatccgaag cacgggctcg
     1501 ctcgacggca acgtcgtggc ccgctcgatc aacgtggaaa aaggcggaac gttctccggc
     1561 ctcttggtca tcggccaaaa aacgtgggaa caggcggaaa tgctgctgcc ggtcgtcgaa
     1621 ggaacgaaac cgcgcactcc gggcaattcg cgtcgcccgc ctccgcaccc cttcggcttg
     1681 ccggcgacct cctagtttgc ggcgaatgca aaaactgctt ccgcgcagtc cttacgagcg
     1741 gctgggtggt tacgtccatt tgccacgctt gatcgacaag gcgcgcctac accgccaggg
     1801 gttgctcgac ggctacaact acaaaaccgt cggctttgac aaacatctcc tcgccttcct
     1861 caaaatcgat ccggacgcct tcgagaaaac ggcgaacgag ctggacgatg acgcggcgat
     1921 tttggactgg gttcgcagga cgggcgtgca gcattcgccg gcggagatcg cgcaatggaa
     1981 ccaggcgatg attgcgcgcc atcccgacac cgcggtgaag aacgcgcgct ttctccattt
     2041 tttgaaggaa gccggcggcg aaggtcgcaa ggacattcga acctacttcg acctcatcga
     2101 gttcgacgaa ggccggctga aggattgaat gtgggagggg cctctgcgcc ccgatccgtt
     2161 gtggcaccct tcgccgatcg gggcgcaaag gcccctccca cattcggcgt tacttcgcga
     2221 attgcgcgaa gatctggttc gcttcttcca gcgtctcttt gctcccgatg tcgtaccagg
     2281 tccccttgat ttgatacgtc ttcaccggcc gccgcgggta gagccattgg ataaaacgac
     2341 cgggctgatc gggattgttg cccgcggcaa tgtaggtggt gaaaagctcg agggtttccg
     2401 gcgagaaata ataaagggcg attcccgtca aagtgctctc gggcttttcc ggcttctctt
     2461 cgaagtgcgt gatgataccg gcggcgtccg tctggatgct ggcgtatttc ttgatcgcct
     2521 cgaggtcgcc gacgtcgtag gtcgcgagcg tcgcggccga gccgcgcgcc cgcgcggcaa
     2581 aatcggccag cggctcgcta aataaattgt cgcccgccac cacaatgagc ggcgcgctcg
     2641 ttagctcttc ccgatcgatg acgaggcgaa tgtcgccgat ggcgccgagt ttatcggcgt
     2701 cgctccgcga accatcgttc acgatcttga aggtcagctt tggttgcgcc agcgcgtagc
     2761 ggtcggccca ggcttgaaaa tcctgggcga atttgtcgtt cgtcaccacg tagattgtct
     2821 cgagatcagg gatcggggcg agattatcga gcacccactc gatcatcggt ttgcccgcca
     2881 cttcgagcag cggcttggct ttgttttccg ttaggggata aaggcgggtg gcgtagccgg
     2941 cggcaagaat gaggacgttc atatgaaagt ggcagcggaa ggtaaacgca tcggtcgttt
     3001 cggcaaaacg gcgccccgcc ccggcccatc gttaggcggg cgcgagcgtg gcctcgtgca
     3061 gttgccgctg cacctgttcg atccgtttcg tctccgtgat ctttccgcgg gtcgtcgcgt
     3121 cggtcacgta aaaggtgtcg atcgcggcac ctttctcggt gctgatacgc gagagggtga
     3181 tgtagacatt gttccgcgcc aggcaggcga cgagatcgta aagcaggccg agccggtcgg
     3241 gcgtctccac ctggatcaag gtgcagctgg gatggctctc gttgccgatc gtgatgcgcg
     3301 tcgggaaatc gagctccggc gcgcgcacgg cggtctcgcg gacgccggcg aggagcggcg
     3361 cgaaattgaa gcgctcggtc tgcagggcgg tgcgcagggt cgattccacg gtgacgatct
     3421 cacgcgcgtc ggtcaccgga ccaagctttt cgtttcgcac ccggaaaaca tcgagcacca
     3481 gattgtcgcc gcggatgaaa atatccgcgc tcaggatatt cagagaggcg acggccaacg
     3541 cgcccgcgat gctggccaaa agatgctggc ggtcccaggt gcagatcgag accacgctat
     3601 ggccctgctc cgggagcgct tcccagcgga gggccggggc gaaggaaggc tcgtcttcca
     3661 ggtaaatcct gcgccaaagc gtgcgaaaca gagcgaggtg cgccacgatt tcttccgcgc
     3721 gaaaggtccg gaaataattg tccggcatgt aatcgaagtg cgcttcgatc tcatcggcaa
     3781 aatcgggtgc gagttggtcg gcgacggcat tgcggagacg gtcgcgctcg attttgccct
     3841 gggcaatgaa tccctcctgg tcctgcaggt aacgcgcagt ggcgtggtaa agatgccaga
     3901 cgagcgattc cttccaatcc gaccaggtgt cagtcgtgcc ctggccatcg gcgagggtga
     3961 gcaacatgag ggcgtcgaga ttcgccttcg acttcacgat attcgcgaac tcgatgatgg
     4021 ttacggggtc atcgacattt cgctgctgcg cgatggtcga gagggtgacg tgatgatcga
     4081 ccaggaggac gagcgagcgg cgttgctcgg aagagagttg gagccgcgcg gcgactccct
     4141 gggcgaagag ggcgctcgcc tcggaatgtg gccgcgcccc gactccttta ccggtgtcgt
     4201 gcaagagcag ggcaagatac agaacatacg gatcggccat cgcctcgaac agtttgcggt
     4261 agggctgcag tttcgggttc tcggtatccc gcaacgcatc gagtttctcg aggcagacga
     4321 gcgtgtgttc atccgcggta taacgatgaa aatattcgtg ctgcacgagg caggtgagcg
     4381 gcccaaactc ggggagataa cgaccgagga aatcgacctc gtgcatcatc cggagaatgc
     4441 gcgccacttc gcccttgcgg cgaagaatac gcgcgaaaat ctcccgtggc gccttcccat
     4501 aacgaaactc gcgcgtgacc agaccaaggc gtcggctgag gagatcggtc aactcggcgc
     4561 tcagctcaag cttgcgctcc tgcacgtgct caaacgcttt cagcaactgc tccggttgcc
     4621 gggtgaagag ctccggatcg gagacgtgca gttgtcctcc gcggctgaag aagttttcga
     4681 agtgttcctc gcgcggcttg cgccggggaa ggagcggaaa gaaaagccgc cgcgtcgtcg
     4741 tggccgtccc ggtggcgaag cgttccgtca gtcgctcggt aagacggaaa atattccgcg
     4801 tgtgcgcata ataatccttc atgaacgcct cgctccgggc gaccggcgtt ttttgggcgt
     4861 aaccgagccg gcgcgcaatc tccggttgca ggctcaggtg caggacatcg gtggtgcgct
     4921 cattggtgta gtgcagctgc gttctcaccc gcagcaagaa atcataggcg agatcgatcc
     4981 ggcggccgtc gccggcgctt agccagtctt tcccgaccag atgtgtggtc gttagggcgc
     5041 cttccttgaa gtaggtgatc cagagaagat tttgataatc gcgcaggccg ccgcagcccc
     5101 gcttcacgtt cggctcctgg agataaatat tgttggcaaa tttttggtgg cgttccgtct
     5161 gatcggccat gcgccccgcg atgtattcct tctcgtgtcc ctgcacacaa tcggcccgga
     5221 agcgcgtgcg gaagcgctgg aaaagcgccg cgtcgcctgc caggtgccgg gcctcgagca
     5281 tcgcggtttt ggtcagcatt tcgcgattgg cctgcgcgac cgcctcgcgc accgagcggg
     5341 tggagtgccc gactttgagg ccgatgtccc agaggaaata aagaacctgc tcgaccgtgt
     5401 gagcgagcga tgccggcacc tcgtcgccgg tatgcgggtg gagaaacatg acgtcgatgt
     5461 cgctccccgg gttgagttcg ccgcggccgt aaccgccgag cgcgagcaga ctcaccggga
     5521 caactttcgc cccgttccct tcctccgccg aagcgctcgc cgccgcaaaa agatggcgca
     5581 gcaggacatc gatgagcgaa gcgcggctcg cgcagacctc gcgtccgccg cccccggcct
     5641 ggtgccgcag gcgcaggcgg tgattttcga tcttgagaaa cttctggtag agcgggagga
     5701 gatgcggcgg cgcggcgtcg cgcgcagggg cgagttcgcg ctccgcgtgt tgcaaaactt
     5761 tatcctgatg atcggacacg ggagggaatc taagttcgaa atccgaatgt cgaaatccga
     5821 aacaacggat tgcagggcgg gcgctccgtc tgcctgagaa agtcatcggg cagcaggcca
     5881 gggcaggcgg cgcgcccgcc ttacaactgg atcgcgtagc gcttcatttt gttgtagagc
     5941 gtcggccgct ggatgccgag caactcggcg gctttctttt tgttgccgtg acattgggcc
     6001 agcgcgtcga ggatggcgtt gcgctccatg tcgtttaggc tccgcgccgg gctctgcgcc
     6061 tgttgggcgg cccgctcggt tttttgcaac accaccggca gccgcacctc ggcgggtaat
     6121 tccttgaccg taatctggtc ctgccgcgcg aggacgaccg catactcgat cgcgttttgc
     6181 agctcgcgca cgtttcccgg ccatccgtac tcgagtagtt tctggaatgc ttccggagag
     6241 atcgccggct ccgctttccc gagctggagc gcgaagcgtt tcacgaacga ggcgaccagc
     6301 ggcgggatgt cctccctccg ttcgcgcagc gggggcagct cgatctggaa agtgttcaat
     6361 cgatagtaga gatcggagcg gagccggttc tccgcgagcg cctggtgcac cgggcgattg
     6421 gtcgcggcca ccacccggaa atccgctcgc aacgtcttcg tgctgcctaa cgggcgaaat
     6481 tcccgctcct gtaaaacgcg cagaaatcgg gtctgcaaat ccgccggcat ctcggaaatc
     6541 tcgtcgagaa aaagcgtgct tccttcggct tccgcgatca tcccgcggaa atcactcgtc
     6601 gcgccggtga aggcgccttt catgtagccg aagagttcgc cctcgatcat cgcctgcggg
     6661 aaagccgcgc aattcaattt caccatcgct ttttcggcgc gacggctccg gccgtgaatc
     6721 acattggcga tcacttcctt gcccaccccg ctctcgccgg tgatgaggac gttggcctcg
     6781 gacggcgcca tcgccgaaat cagttcatcg atttcctgca tcaccttgct cttgaaaacc
     6841 ggcgcaaggc gcgtctcggc ctgcgcgagc cgcttcttta atcgctgggt ggtggcgcgc
     6901 agctcgatcg tctcgcggag gagcgattgc ttctcgagcg ctttctcgac cagactgagc
     6961 agctcttccg gcgcatacgg cttgctgatg aaatgaaacg ccccgcgctt gatcgactcg
     7021 atcgcgttgt tcaacgaatc gtgcgcgctc agaatgatga cctgcgtgtc gggagagtcg
     7081 gctttgattt gctcgagcat ctgcaggccg tcggcgtccg gcagttgcag gtcgagcagg
     7141 accagcgccg gcgcggtgct ctggagtaaa cgcatcccgg tggatgcatt cgcggccatc
     7201 tcggcctgat aaccatgccg gccaagcaac gcttcgagcg tcatcaggat gggacgctcg
     7261 tcgtcaatga tcagaatgcg gcggttctcc gtggtcatcg aaaatctccc ggccggcggc
     7321 gcgacagtct aggaaacaag caggggcaga gagacggtcg tccgcaggac cgagccggcc
     7381 ggatcaaatt gggcgcgaag attgccatgg tgcgcctcga aaatactgcg cgcacggaag
     7441 aggccaagac cataatgccc attccgcatc tgtcggaacg ggcgcccgcc ccagttttcc
     7501 gtcgtgccgt cgaacttcgt cttcggttcg cagagggtaa actcgaccgt ttgatcgctc
     7561 cggcccgccg caaaaacgag cgcgccttcc gcccggtcat gggtgccggc gttggtcagg
     7621 agttcgacga acgcttcctg caaaagttgc ggatcaattt ccagggcttc gccgcctaac
     7681 gaaatctgcc actcgatcgt ggccgatcgt tccggttgat cgagcgcgag tttggcgcgc
     7741 agatcttcga taaattccac cgccggatag cgcatcaggt tcggctcgat cgccgccagc
     7801 gagtgcgcaa gccggtgcag gtgcgcgccc aactcgcccg tcatttcacg cagtcgtttg
     7861 atctcgttct ttaattccgg cccttcggca atttcaccca ggaacgccgc gtgcagttcg
     7921 accgcgttga ggtagttgcg cagatcgtga ttcagctggc cgatgaactt tgtcacctcc
     7981 gtccacgaaa tgttcgggtc cgcttgcgcc atgaaagagc cccgagcgtt gccggttcgc
     8041 gggcggaacg aatctaggcg ctgagtcacg gtccgctagt tgatccgcga aaatttttcg
     8101 ctgccgggcc cctcgccccc ggcctgaagc gacgaatcgt tggccgcgag gtggcgcagg
     8161 acgtggtaaa catcctccgg atccgcgcca atcgcctgcg cgattccgtc ggccgtgatg
     8221 ccggtcgcgg aaagttgcgc gcggaccttc ggctgcaatt gcaggatccg cgtcgccgcc
     8281 ttttttccgg cctccacgcc gggttgatga taggcgttca cgttcacgag cgatccgtag
     8341 aacccgaccg cgcgctcata aagcgcgatc aatgcgccga cctgaaaggc atccacctgc
     8401 ggaatgctga gggtaatcga gtcgcgcccg ttctcataca gtgcggcgcg cgtgccgcgc
     8461 aggaagccct gcaaataatc accgctcgtg acgccttcct cgacttcgaa ccggccgacc
     8521 gcgcgatcct ggcgcgcctc gatgaaggtg gcgaaaaaat tcggcacgcc gtcccgcaat
     8581 tgctgcacgt aggcgtgttg atcggtcgag cctttgtttc cgtaaacggc aattccctgg
     8641 tggacaacct ggcccgcgag atccttctct ttgcctaacg actccatgac gagctgctgg
     8701 aggtacttgc tgaagagcgc gagccggtct ttgtagggca ggatgaccat gtctttttgt
     8761 ccgcggccgt cgccggcata aaaccacatc agcgcgagca gcatggccgc gttggtcgcg
     8821 cgctccggct ggcgcgtgtg cgcatccatc gccgccgcgc cctttagaaa atcgtcgatc
     8881 gcaaaaccct ccagcgccgc gggcaccaac ccgacggcgc tcatcaccga ggtgcggccg
     8941 ccgacccaat cgtgcatcgg aaagcgcgcc agccagccgg ccttttctgc gactttatcg
     9001 agttcgctcc cgacgcccgt caccgccacg gcgtgcttgg gaaattgcaa cccggctttc
     9061 gtgaaagccg cggccgcctc cagcatcccg ttgcgcgttt ctttcgtgcc cccggatttc
     9121 gagatcacga cggcaagcgt ttgcgccaac ccagtgccga ttttctcgag cacccggtcg
     9181 aagccatcgg gatcggtgtt gtcgaaaaag aaaatatcca tcggatcgcg tgtcgcaccg
     9241 agggcttcgg cgatgaactg cggtccaagc gcggagccgc cgattccgac cagcagaacg
     9301 tgagtgaagc gctcgccccg ttcgtttgcc acctggccac cgtggacggc acccgcgaac
     9361 tgtttgatgc gcgcgttggt ttcaacgata tcggcgcgca actgttcgtt aggcgagagc
     9421 gccggattgc gcagccagta gtgcccgacc atgcggcctt cgtcgggatt ggcgatgccg
     9481 ccgtcctcga gcgcgcgcat cgcggcgaag gcttgctcga ccctgggctg catcttctcg
     9541 aggaagccat cgggaaaccg catccggctg atgtcgaggg agaagccgag gtcgtcgtag
     9601 cgcaggtagt attcctggaa tcgctgccag agagaattcg cgggcatagg ggagcttaac
     9661 caacaatcaa attgtagggc aagcgctccg cttgccgaga ataaagacag ggcaggcgaa
     9721 gcgcccgccc tacaatgcct aatccttctc tttccgccct tcttcgcgct ccttgtcctc
     9781 gtggtcgctt tgcttgtctt cgtcccagcc aatcttgcgg cccttatttt gctcttcgag
     9841 ccaggccatc agcggctgga aataatcgac catcgcgcgc gtggagagat cttcgcccgt
     9901 cgcttccttc agcacttttc gccagtcttc ggtgccgccc tt
//
LOCUS       DATMNS010000254         8493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3082511, whole genome shotgun sequence.
ACCESSION   DATMNS010000254 DATMNS010000000
VERSION     DATMNS010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3082511"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VNW28_08950"
     CDS             complement(<1..296)
                     /locus_tag="VNW28_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10097.1"
                     /translation="MTFFDELKRRKVYRVAVAYVIAAGGIIQLASAVFPAWELPNWSL
                     RLVIVLLLVGFPIALILAWAFDITPTGIQTTPALPAAGDATSRHRRRNVFLLGAA"
     gene            complement(358..2382)
                     /locus_tag="VNW28_08955"
     CDS             complement(358..2382)
                     /locus_tag="VNW28_08955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA10098.1"
                     /translation="MSSFFDELKRRKVYRVAVAYVIAAGGIIQLASAVFPAWELPDWS
                     LRLVIVLLLIGFPIALILAWAFDVTPTGIQATPALSTSPHRRRNVFFLGAAGLVVAVT
                     AGFFLLPHVAAKKVDKSIAVLPFENLSEDKDNAFFADGIQDDILTNLSKIGDLKVISR
                     TSVMGYRGQEKNVRQIGKELGVSAILEGSVRREGNRVRVNVQLINAENDQHLWAEDYD
                     RDLTDVFAIQTDLAQKIARELQAQLSPSEEAQMTRKPTENGEAYLAFVEAHNLQTSLE
                     DRGKLNQAAQLYERAIQLDPKFVLAIANLSIVHSWVYHNFEPTDAERDLAKKYADRAL
                     ELAPDSPEAHLAGGFSLYYGTRDYEGALRELRLAQVGLPNDAGVYLLIGAIQRRQGKW
                     KESTANLEKAVSLDPNDTWPLQNLFFNYQMQRDFEAANRVIERALAINPKSVSLWGLK
                     AQLAINARGDLTVAQNALAKFEQAQAPGEVPKNLDSVTVAEYGLGKANILTFRRKYPE
                     ALAGLRQIPQEALTSLPDAFCEARLLEGILLEKMGQGAEARVAFSKAKESAEAAVREA
                     PNEASRHAKLGRALAYLGEKEAAITEAKRATELLPESVDAFSGPGMTQALAEVYMVTG
                     DHAKAIELVDGLLSRPGELTVAFLKIDPILDRLRDDPEFQKVLAKHEAKS"
     gene            complement(2460..3395)
                     /locus_tag="VNW28_08960"
     CDS             complement(2460..3395)
                     /locus_tag="VNW28_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXA10099.1"
                     /translation="MASTINFLRLLADPTRLRLLLLLEEEELSVAELQDVLGMGQSRI
                     SSHLAQLKRAGVVEDRRAGKNVYYGLTSGRKLNPQHAKVSELTRELAREMPETQRDRT
                     ALKIVLRKRQDKAREYFDQLAGRFGRSYCPGRSWQALAHTMIALLPPLTVADLGAGEG
                     TLSQLLAKKARQVIAVDNAPKMVEFGSQLAQRHGFKNLEYRLGDIEDPPIKINSVDLA
                     FLSQALHHAIHPERAIAAAHRILKKGGRLIVLDLLSHRFEKARELYADHWLGFSEVQL
                     HQWLEKAGFTQIEVSVVSREKESPHFQTVFASGMK"
     gene            3551..4750
                     /gene="metK"
                     /locus_tag="VNW28_08965"
     CDS             3551..4750
                     /gene="metK"
                     /locus_tag="VNW28_08965"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HXA10100.1"
                     /translation="MLRRFIFASESVGEGHPDKVCDTISDAVLDACLRHDPKSRVACE
                     TFAKSHTVIVGGEITIPSLPHGTSLEAIVPIDQIVRAAVREIGYVNDDDVFHADKIDI
                     QNIITNQSADIAQGVDAREAEGKGHAEQGAGDQGLMFGFACDETPELMPAPIMFAHRL
                     GRELTRIRKSGKARWLRPDAKSQVSVIYDGGRPVGVSNVVVSTQHSKDVKHSEIRSFI
                     IEEVIRKVLPNEMLTDATEYLINPTGNFVIGGPEGDTGLTGRKIIVDSYGGWGRHGGG
                     AFSGKDPSKVDRSAAYMARWVAKNVVAAGLATRCEIQFAYAIGYPDPVSMNINTFDTN
                     TVPEEAIERAVNETFSFKPAHIIAQLDLLRPIYSKTTNYGHFGKDDPDITWERTDKTA
                     ALKKAAR"
     gene            4770..6203
                     /gene="ahcY"
                     /locus_tag="VNW28_08970"
     CDS             4770..6203
                     /gene="ahcY"
                     /locus_tag="VNW28_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXA10101.1"
                     /translation="MSSKTISKIQTNDYKVADISLADWGRKEISIAEKEMPGLMAIRE
                     KYAPGKPLQGVRVTGSLHMTIQTAVLIETLVDLGANVRWASCNIFSTQDHAAAAIAKA
                     GVPVFAWKGESLAEYWWCTNQAISFPEGKGPQLVVDDGGDVTLLIHKGYQLEEGSDWA
                     KSPASSKEEQCVKDLLLEVQRENPFRWHELVKEWRGVSEETTTGVHRLYKMQQQNQLL
                     VPAINVNDSVTKSKFDNLYGCRHSLVDGLNRALDVMIAGKVAVICGYGDVGKGCAQSL
                     RGQGARVVITEIDPINALQAAMEGFEVTTIEDTLGRGDIYVTTTGNVDVITLAHMERM
                     KDQAIVCNIGHFDNEIQVEPLNERKDVQRTNIKPQVDKYTFPDGHEIFMLAEGRLVNL
                     GCATGHPSFVMSNSFSNQVLAQLDLWRNKDTYQIGVYVLSKKLDEEVARLHLQKIGIK
                     LTTLTKKQADYLGVAVEGPYKSEHYRY"
     gene            6291..6731
                     /locus_tag="VNW28_08975"
     CDS             6291..6731
                     /locus_tag="VNW28_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10102.1"
                     /translation="MRLPFLTLTILLSVITLAAPSFGAGGDQQESELEAARHQREHPA
                     QSATPPVAVAQAPYNLGQAIFNGTYKFGKAKANHVPEKAHRLGILQGGLPAAEQKHFH
                     PQELAGHLNDREMNALEYYIQVKFGAFVTVAPSWAKEEPPIKVK"
     gene            6814..7272
                     /locus_tag="VNW28_08980"
     CDS             6814..7272
                     /locus_tag="VNW28_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10103.1"
                     /translation="MRPLFVRLSAVALVTTIPALTFGGMGYTTPKITSITEPNGLHGE
                     NPPANPATHVVIAQAPYNLGQAIFNGTYKFGKAKVNHVPEKAHRLGILQGGLPAGEQK
                     HFHPQELAGHLNDREMNALEYYIQVKFGVFVTVAPSWAKEEPPIKVINSK"
     gene            complement(7334..8434)
                     /gene="cax"
                     /locus_tag="VNW28_08985"
     CDS             complement(7334..8434)
                     /gene="cax"
                     /locus_tag="VNW28_08985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015368 - calcium:monoatomic cation
                     antiporter activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HXA10104.1"
                     /translation="MRKSSWSLTLLDLLLVAGPVAIALRYVPAWRNETLLFLVAAIAI
                     VPLAGWMGRATEQLAARSGSGIGGLLNATFGNAAELIIGLVALSKGLTGVVKASIAGS
                     IIGNLLLVLGASLLVGGLRHPKQTFNQTAARVSATSLGLAGIALLIPTVFHVAAAGPS
                     AGRTATIEARLSVAIVLVLFLTYFLSLLFHLVTHRELFEGSSLAGQDDETPWPLWQSV
                     TALAIATAFVAWLSEFLVGSIEAARRTLGLPEIFVGVVVIAFVGNAAEHSTAVWSAWK
                     NKMDLSLGIATGSSLQMALFVTPVLVLASYGFGRPMNLEFSLPEIASIIFSIWIVGKI
                     SGDGESNWYEGVQLLAVYVIIALLFFFLPASP"
BASE COUNT         1877 a         2571 c         2461 g         1584 t
ORIGIN      
        1 gccgcgccta acaaaaagac gttgcgccgg cggtgccgac tggtcgcgtc gccggcagcc
       61 ggaagagcgg gcgtcgtctg gatgccagtt ggggtaatgt cgaaagccca agccaggatg
      121 agcgcgatcg gaaatccgac cagcaggagc acaatgacaa ggcgcagcga ccagttgggc
      181 aattcccacg ccggaaaaac cgctgacgcg agttgaatga tgccgccggc cgcgatcaca
      241 taggcaacgg cgacgcggta aaccttgcgc cgcttcagtt cgtcgaaaaa ggtcacgccc
      301 aatcaatccg tttgctgccg gggaattcaa gcgcagacaa tggccgataa agcggcgcta
      361 actctttgct tcgtgtttcg caagcacctt ctggaactcc ggatcgtcgc gcaatcgatc
      421 caagatcgga tcgatcttga gaaaggccac ggtcaactcc cccggccggc tgagcaatcc
      481 atccaccaac tcaatcgctt tcgcatggtc cccggtcacc atgtagacct cggcgagcgc
      541 ctgggtcatc cccggcccgg aaaaggcatc gaccgattcg ggcaacaatt ccgtggcgcg
      601 ttttgcttcg gtaatagccg cctctttttc cccaaggtaa gcgagtgcgc gccccagctt
      661 cgcgtggcgc gaagcctcgt taggcgcttc ccgcaccgca gcctcggccg attcctttgc
      721 tttcgagaaa gcgacgcgtg cttcggctcc ctggcccatt ttctcgagca ggatgccctc
      781 gaggaggcgg gcctcacaga atgcatccgg cagagaggtc agcgcttcct gcggaatttg
      841 gcggagcccc gcaagagctt cgggatattt gcgccgaaac gtcaaaatat tcgccttccc
      901 gagcccatac tcggcgaccg tcacggaatc cagattcttg ggcacttcgc ccggggcttg
      961 tgcctgttcg aatttggcca gagcattttg cgcgaccgtg aggtcaccac gcgcgttgat
     1021 cgccagttgg gctttaagtc cccagaggct gactgatttc ggattgatgg ccagagcgcg
     1081 ctcgatgacc cgattcgccg cctcgaaatc acgctgcatc tggtaattga aaaacagatt
     1141 ctgcaacggc caggtgtcat tcggatcgag gctgacggct ttctccaggt tagcggtcga
     1201 ttctttccac ttcccctggc ggcgctgaat cgcgccgatc aggagataga ccccggcgtc
     1261 gttaggcaag ccgacctggg caaggcgcaa ttcgcgcagc gcgccctcgt aatcccgggt
     1321 gccgtagtag agcgagaaac cgcccgcgag gtgcgcttcg ggcgagtcgg gcgccaactc
     1381 cagagcgcgg tcggcgtact ttttggccag gtcacgctcg gcgtcggtcg gctcgaagtt
     1441 gtgataaacc cagctatgca cgatcgagag attggcgatg gcgaggacaa acttggggtc
     1501 gagctggatg gcacgctcgt aaagttgcgc cgcttgattc aatttgccgc ggtcctccag
     1561 cgacgtctgc aagttatggg cttcgacgaa agcgagatag gcctcgccgt tctcggtcgg
     1621 cttgcgggtc atctgcgcct cttcgcttgg ggaaagttgc gcctgcaact cacgcgcgat
     1681 cttttgcgcc aggtcggtct ggatggcgaa gacatccgtc aggtcgcgat cgtaatcctc
     1741 cgcccacagg tgctgatcat tctcggcatt aatcaactgc acgttcaccc gcacccggtt
     1801 gccctcgcgc cgcacgctcc cttcgaggat ggctgagacg cccaattcct ttccgatctg
     1861 gcgcacgttc ttctcttgcc cgcgatatcc catgaccgag gtgcgcgaga tcaccttcaa
     1921 gtcgccgatc ttcgagaggt tcgtcaggat gtcatcctgg atgccatccg cgaaaaaggc
     1981 gttgtcctta tcctcactga gattttcgaa cggcaggacc gcgatcgatt tgtccacctt
     2041 cttcgcggcg acgtgcggca gaaggaagaa gccggccgta accgccacga ccagcccggc
     2101 cgcacctaag aaaaagacgt tgcgccgccg atgcgggctg gtcgagaggg caggcgtcgc
     2161 ctgaatcccg gtcggggtaa cgtcgaaagc ccaggcgagg atgagggcga tgggaaagcc
     2221 gatgagcaag agcacaatca cgagccgcag cgaccagtca ggcaattccc acgccggaaa
     2281 gaccgccgac gcgagctgaa tgataccgcc cgccgcgatc acgtaagcga ccgcgacgcg
     2341 atagaccttg cgccgcttca gttcgtcaaa gaaggaactc atcgccccct aatctcagtt
     2401 ggcccatggg tccggtgcaa gtcttcccgg cgtcgcaagt gcacgccgtc aagacctgct
     2461 cacttcatgc cggaggcgaa gaccgtctgg aaatgtggac tctccttctc gcgcgagacc
     2521 acgctcactt cgatctgcgt aaagcccgct ttctccagcc attgatgcag ctgcacttcg
     2581 ctgaagccaa gccagtgatc ggcgtaaagt tcacgcgcct tttcgaaacg atggctcaac
     2641 agatcgagca cgatgagacg gccacccttc ttcaaaatgc ggtgggccgc ggcgatcgct
     2701 cgctcgggat ggatcgcatg gtgcagggcc tgactcaaaa acgcgaggtc gaccgaattt
     2761 attttgatcg gcggatcctc gatgtcgcct aaccgatatt cgagattctt gaaaccatgg
     2821 cgctgcgcga gttgcgatcc gaactcgacc atcttcggcg cgttatcgac cgcgatcacc
     2881 tgacgcgcct tcttcgcgag caactgcgaa agggtcccct cgcccgcccc gagatcggcc
     2941 acggtgagcg gcgggaggag cgcgatcatg gtgtgagcca gcgcctgcca cgagcggccg
     3001 ggacagtagc tgcggccgaa ccggcccgcc agttgatcga agtactcccg cgccttgtcc
     3061 tgtcgtttac gcaggacgat ctttagggca gtccgatcgc gctgcgtctc gggcatttcg
     3121 cgggccagtt cgcgggtcag ctcgcttact ttcgcgtgct gcggattgag cttccggcca
     3181 ctcgttaggc cataatagac attcttcccg gcgcgccgat cctccaccac gccggcgcgt
     3241 ttcagctgcg cgagatggct cgagatgcgc gattgcccca tcccaagaac atcctgcaac
     3301 tccgcgaccg agagctcctc ctcctcgagc aggagaagga gccggagccg ggtcggatcg
     3361 gcgagcaagc gcaggaaatt tatggttgac gccatgaact gagaccacga tatatcgacg
     3421 tatcatgatt ggtcaatatg tccattgctg cctgctcgca gactcaaggg ttcaagtttc
     3481 agagttccgc gcgctgtggc cgtccctccc ctgaatcctg aaaccctgaa tcccaaaccc
     3541 ttttcttcat atgcttcgac gtttcatttt tgcttccgaa tccgttggcg aaggccatcc
     3601 ggataaagtt tgcgacacca tctccgacgc cgtgctcgat gcctgcctcc ggcacgaccc
     3661 gaaaagccgc gtggcctgtg agacctttgc caagagtcat acggtgatcg tgggcggcga
     3721 aatcacgatc ccaagtctgc cgcatggcac ttcgctggag gccatcgtgc cgattgatca
     3781 gatcgtgcgc gctgccgtgc gcgagattgg ctacgtcaac gacgatgacg tttttcacgc
     3841 cgacaagatc gacatccaga acatcatcac caaccaaagc gcggacatcg cacaaggggt
     3901 cgatgcgcgc gaagccgaag gcaaggggca cgccgagcaa ggcgcgggcg accagggctt
     3961 gatgtttggt ttcgcctgcg acgaaacgcc ggaattgatg ccggcaccga tcatgttcgc
     4021 ccaccgcctc ggccgcgaac tcacgcgcat tcgcaaaagc ggcaaggccc gctggctgcg
     4081 accggatgcc aagtcgcagg tctcggtcat ctatgacggt ggccggccgg tcggcgtctc
     4141 caacgtcgtc gtctcgaccc aacactcgaa ggacgtgaag cattcggaga ttcgcagctt
     4201 catcattgaa gaggtcatcc ggaaggtatt gcctaacgaa atgctgaccg acgcgaccga
     4261 gtacttgatc aaccccaccg gcaactttgt tatcggcggg ccggagggcg acactggcct
     4321 gacgggccgc aaaatcatcg tcgacagcta cggaggctgg ggtcggcacg gcggcggcgc
     4381 attttccggc aaggatccga gcaaagttga tcgcagcgcg gcttacatgg cgcgctgggt
     4441 ggcgaagaat gtcgtggcgg cgggcctcgc cacgcgttgc gaaatccaat tcgcttacgc
     4501 catcggctat ccggaccccg tcagcatgaa catcaacact ttcgacacca ataccgtgcc
     4561 ggaggaagcg atcgagcggg cggtcaatga gacgttctcc ttcaaacctg cgcacatcat
     4621 cgcgcaactc gatttgctgc ggccgatcta ttccaagacc acgaactatg ggcatttcgg
     4681 aaaggatgat ccggatatca cctgggaaag aaccgacaag accgccgcgc tgaaaaaagc
     4741 ggcaagataa ttttcatctt catcaattta tgagctcaaa aacgatcagc aaaattcaaa
     4801 cgaacgacta caaagtcgcg gacatcagcc tggccgattg gggccgaaag gaaatctcga
     4861 tcgcggaaaa agagatgcct ggcctgatgg cgatccggga aaaatacgcc ccgggcaaac
     4921 cgctccaggg cgtgcgcgtg accggatcgc tgcatatgac gattcagact gccgtcttga
     4981 tcgaaacgct ggtcgatctc ggcgcgaatg ttcgttgggc gagctgcaat attttctcga
     5041 cgcaagatca cgccgcggcc gcgatcgcca aggcgggcgt gccggtcttc gcctggaagg
     5101 gcgaaagcct ggccgaatac tggtggtgca ccaaccaggc gatttcgttt cccgagggca
     5161 agggtccgca gctcgtcgtc gacgatggcg gcgacgtcac gctactgatt cacaaaggct
     5221 atcaactgga agagggcagc gattgggcga aatcgcctgc gagcagcaag gaagagcaat
     5281 gcgtcaagga tttgctcctc gaagtgcagc gcgagaatcc gttccgctgg catgagctgg
     5341 tcaaggaatg gcgcggcgtt tcagaagaga cgacaaccgg cgtgcatcgg ctctacaaga
     5401 tgcagcagca aaatcagctg ctcgtgcccg cgatcaacgt caacgattcg gtcacgaaat
     5461 cgaaattcga caacctttat ggctgccgcc attcgctggt cgacgggctc aaccgcgcgc
     5521 tcgacgtcat gatcgccggc aaagtcgcgg tcatctgcgg ctacggcgat gtcggcaaag
     5581 gctgcgcgca atcgctccgc ggccagggcg cgcgtgtcgt catcaccgag atcgacccga
     5641 ttaatgcgct ccaggcggcg atggaaggtt tcgaagtgac cacgatcgag gacacgttag
     5701 gccggggcga catttacgtc actaccacgg gcaacgtcga cgtcattacg ctcgcccaca
     5761 tggagcggat gaaggatcag gcgatcgttt gcaacatcgg ccatttcgac aatgagattc
     5821 aggtcgagcc gctgaacgaa cgcaaagacg tccagcgcac gaacatcaag ccgcaggtcg
     5881 acaagtatac cttcccggat ggacacgaga ttttcatgct ggccgaaggc cgcctggtga
     5941 atctcggctg cgccaccggg catccgagtt ttgttatgtc gaactccttt tccaaccaag
     6001 tgctcgcgca gctcgatctc tggcggaaca aggacactta ccaaatcggc gtttacgtct
     6061 tgtcgaaaaa actcgacgag gaagtggcgc gactgcactt gcaaaagatt gggatcaaac
     6121 tcactacgct cacgaaaaaa caggcggatt atctcggcgt cgcggtcgaa ggaccctaca
     6181 aatccgagca ctatcgctat tgagcgggcg ggctggaact ggggggaacg cgtcgcacgc
     6241 cgaaggcaag gaaatgcgtg ctttttttgc gcaagttagt attttggcgc atgcgattgc
     6301 cattccttac actcacaatt ctcctctccg tcataaccct cgccgcgccc agcttcgggg
     6361 cgggtggcga ccagcaagaa agcgaactgg aggccgcgcg ccaccagcgc gaacatccgg
     6421 cccagtccgc gacgccgcct gtcgcggtgg cacaggcacc ctataacctt ggccaggcga
     6481 tttttaacgg cacctacaaa ttcgggaagg cgaaggccaa tcatgtcccc gaaaaggcgc
     6541 atcgtcttgg tattctgcag ggcggtttgc cggcggccga gcagaagcac tttcaccctc
     6601 aggagctggc cggccatttg aacgaccggg agatgaacgc cctcgaatat tatatccagg
     6661 taaaattcgg agcgttcgtg acggtcgcgc cctcctgggc caaagaagaa ccgccgatca
     6721 aagtcaagta agcgtcgcac cctaacgacg agcaaccgtt ccgcggcgga gaaagagcgt
     6781 gcatcactcg tgagagccca atatcttaga cgaatgcgcc cgctgttcgt gagactgtct
     6841 gcggttgcgc tcgtaacaac cattccagca ctcaccttcg gcggaatggg atacacgacg
     6901 ccgaaaatta cgtctatcac ggaaccgaac ggtttgcacg gcgagaaccc gccggcgaat
     6961 cctgcgaccc acgtcgttat cgcgcaggca ccgtataacc ttggccaggc gatttttaac
     7021 ggcacctaca aattcgggaa agcgaaggtc aatcatgtcc ccgaaaaggc gcatcgtctt
     7081 ggtattctgc aaggcggttt gccggcgggc gagcagaagc actttcaccc tcaggagctg
     7141 gccggccatt tgaacgaccg ggagatgaac gccctcgaat attatatcca ggtaaaattc
     7201 ggagtgttcg tgacggtcgc gccctcctgg gccaaagagg aaccgccgat caaagtgatc
     7261 aattccaagt aagcgtccaa ccctgacgac tgccacgccg aggaatcaat tcggcgacaa
     7321 ttcctccttg ccactagggc gacgcaggta ggaagaaaaa gagcagcgcg atgatgacgt
     7381 aaaccgcgag cagctgcacg ccttcatacc agttgctctc gccgtcaccg ctgatttttc
     7441 cgacgatcca gatcgagaaa ataatcgaag caatctccgg cagagaaaat tccaaattca
     7501 tcggccttcc aaacccgtaa gaggccagca cgagcaccgg cgtgacgaaa agtgccattt
     7561 gcaggctgga cccggtcgcg atcccgagac tgagatccat tttgtttttc caggcgctcc
     7621 agaccgctgt cgagtgctcg gcagcgttcc cgacaaaggc aattacgacc acaccgacaa
     7681 agatttcggg caaacccaaa gtgcgccgcg ctgcctcgat ggatccgact aggaactcgc
     7741 tcagccacgc cacgaatgcc gttgcgatgg cgagtgcagt gaccgattgc cagagtggcc
     7801 agggcgtctc gtcgtcctgt ccggccagcg aactcccttc gaaaagctcg cgatgggtga
     7861 cgaggtgaaa tagaagcgac agaaaatagg tcaaaaaaag gaccaggacg atggccacgg
     7921 aaagccgggc ctcgatcgtc gccgtccttc ccgccgaggg tcccgccgcc gccacgtgaa
     7981 agacggtcgg gataaggagc gcgatgcccg ccagcccaag tgaggtcgcc gaaacgcgcg
     8041 ccgcagtttg gttgaaagtc tgtttcggat ggcgcaaccc accgacgaga agcgaagcgc
     8101 cgagcacgag caaaaggttg ccaatgatcg aacccgcgat cgacgctttg acaacgccag
     8161 tgagtccttt gctcagcgcg accagaccga tgatcagttc tgccgcgttc ccgaaagtcg
     8221 cgttaagcaa accgccgatg ccggaaccgg agcgcgccgc cagttgctcg gtcgcgcgac
     8281 ccatccagcc ggccaacggc acgatcgcga tcgccgcgac gaggaagaga agggtttcat
     8341 tcctccacgc cggcacgtag cgcagcgcga tcgcgactgg cccggcaaca agcaacaaat
     8401 cgagcaacgt tagcgaccaa ctggattttc gcacgcgcgc catgcatcat aggagcttgg
     8461 cccgccccac cgctcaagaa acttcgtcgt cct
//
LOCUS       DATMNS010000255         4502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3447512, whole genome shotgun sequence.
ACCESSION   DATMNS010000255 DATMNS010000000
VERSION     DATMNS010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4502
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3447512"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VNW28_08990"
     CDS             <1..303
                     /locus_tag="VNW28_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10105.1"
                     /translation="KAPFGQARGMPLAAVQFSLPTGATPAEWNAAPALCSDGSLIREH
                     VKGVAKLIHVERLGKQQVDAEIFVGVDILLREMSREDGNLAAEILHTQLAHEFETR"
     gene            complement(745..1173)
                     /locus_tag="VNW28_08995"
     CDS             complement(745..1173)
                     /locus_tag="VNW28_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10106.1"
                     /translation="MNYNELIQLYFDRSNAMQGYWNLYVLIVGGLLAFSSLRKQSAAV
                     TTALVCLLFALFAYNNLDAMKDVTAQRFALLDAIRQFDASGGATTNPKPVRDLLEPTL
                     TPATYASVKMTHLTSDILTIVALVAMEIRRRNLRDGARVP"
     gene            complement(1186..1767)
                     /locus_tag="VNW28_09000"
     CDS             complement(1186..1767)
                     /locus_tag="VNW28_09000"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXA10107.1"
                     /translation="MSVLFLVVYGSCIWITARRSDVGVLYFRWERAIPFVPFMILPYL
                     SIDLFFIAAPFLFRTDRELRVFVWRVSVAIVVAGCFFLFVPLRYAFPRPHADGWLGFL
                     FDWFRGLDAPFNLFPSLHAALLLFLVDAYARHLRGFVRSAVMSWFVLIGLSPLLTHQH
                     HLIDILGGFALAAGCFYLIGGKFSLALGEGRPS"
     gene            complement(1818..3542)
                     /locus_tag="VNW28_09005"
     CDS             complement(1818..3542)
                     /locus_tag="VNW28_09005"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HXA10108.1"
                     /translation="MEAAEQTMTSWDGTELFYRAWLPKGKRAEQALFLFHRGHEHSGR
                     WQETVEALGLEDVAIFAWDARGHGRSPGARGAARDLTDVIKDVATFVRHIEERHGIAC
                     ENMIVLAHSLAAVTVGAWVHDYAPPIRALILATAAFDVRLYVPLAIPALRLHQRTVGA
                     GQVTSYVRAKMLTHDPIEAARYAADPLIFRQIAINVLLDLHDTARRLLADAGAIHVPT
                     LMLAAGRDWVVSLDAQREFFNRLSSPLKRMHVFPAMYHSIFHEKDRAQVIERLREFIV
                     ERFAQAPLAVSLRAADKYGHTWEEYEKLKLRGGPQFLAIKAALKIGGRLSKGIDLGWQ
                     SGFDSGRSLDYVYENRAQGSSRLGKFIDSNYLNSIGWRGIRQRKVNLEKALCEAIEKL
                     HADGRAVRILDIAAGAGRYVLETMRALREIPISATLRDNELENIEAARALAAEFGLEN
                     VAVEQGDAFDRAALAALRPRATIGIISGLYELFPSNDTVLESLGGLAAVLEPGGYLIY
                     TNQPWHPQLEFIARVLRNRAGERWIMRRRTTAEIDELVRAAGFEKCAMEIDRWGMFSV
                     TLARRALT"
     gene            complement(3529..4257)
                     /locus_tag="VNW28_09010"
     CDS             complement(3529..4257)
                     /locus_tag="VNW28_09010"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXA10109.1"
                     /translation="MRRWGYASALVFERTAVERLRRFPREPDRFVSSLRALLAMVIRG
                     LLRIYLRFEIIGRENLPNDESFVLVANHASHLDTLCLLAALPLRRLPRAFPVAAEDYF
                     FRGLPRTWFAAVVMNAMPFGRRMRVRENLAICAQLLAQPGTVLIIFPEGTRSMTGETQ
                     PFKSGIGALLAGRAVNVLPCQVQGAFRAWPKGRRFPRPAKVRLIIGAPRNYAACSLEK
                     SDLCAIASELQGAVRRLGEADGSG"
     gene            complement(4254..>4502)
                     /locus_tag="VNW28_09015"
     CDS             complement(4254..>4502)
                     /locus_tag="VNW28_09015"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXA10110.1"
                     /translation="LPWALWFTFPHFGPADLLAVGLIVGVGGQLGDLSLSVIKRDLAA
                     KDWGAAIPGHGGVLDRIDSLIFVAPLFMAMAGHYYPLR"
BASE COUNT          942 a         1446 c         1378 g          736 t
ORIGIN      
        1 aaggctccgt ttggtcaagc acgcggcatg ccgctcgcgg cggttcagtt ttccctcccg
       61 acgggggcga cgccagcgga gtggaatgcc gctcccgctt tatgcagtga tggaagcttg
      121 atccgcgagc acgtcaaggg cgtggcgaag ctcatccatg tcgaacggct tggaaagcaa
      181 caggtcgacg ccgagatctt cgtaggcgtg gacattctgc tccgtgagat gagccgagag
      241 gacggcaatc ttgccgccga aattttgcac acgcagttgg cgcacgagtt cgagacccgt
      301 tagccgaggc atactgtgat cagtcaggac cacgtcgaac ggtcgcggag aagcggcgat
      361 tttggcgagc gcctcttcgc cgttcgccgc ggtcgtcacc ttgcagcgcg gcccgcataa
      421 gaccagcgcc agcaactgag caaccgcggt ctcgtcctcg acagcaagga ttttcacacg
      481 ttccttcatg tctcggcact aaactagcaa gagacgcgcc gcgggcgggc cggcaacgat
      541 ttttccggcg cggcggaaca ttctcctgcg attttggaaa aagccgcaaa ccctcggtgc
      601 gatgacaggg acgaggacgc cccttcaccc gctttccgtc gcgtggcctg cgaaacgcac
      661 acgatcgcgc gctccactaa aatcgaattt tcccgacctc ctcggagcgc cccgctcggc
      721 caggcgataa atcaggcaaa atcgttaagg cacgcgcgct ccgtcccgca ggttgcgtct
      781 ccgaatttcc atcgcgacga gagccacaat ggtcagaata tcgcttgtca ggtgggtcat
      841 tttcacgctc gcatacgtgg ccggcgtgag cgtcggttcg agcaggtcgc gcaccggttt
      901 cggattggtc gtcgcgccgc cactggcatc aaactgccgg atcgcatcga gcagggcgaa
      961 acgctgcgcc gtgacgtcct tcatcgcgtc gaggttgttg taggcgaaaa gtgcgaacaa
     1021 gagacagacc agcgcggtgg tgacggcagc gctctgtttg cgcaaggatg aaaacgccag
     1081 gagaccgccg acgatcagga catacagatt ccaatatccc tgcatcgcgt tcgagcgatc
     1141 gaagtaaagc tgaatcaatt cgttgtagtt catggctggg cgcgatcatg acggtcgccc
     1201 ctcgcccaaa gcgaggctaa actttccgcc gatcagataa aaacaacccg cggccagggc
     1261 aaagccgccg aggatgtcga tcaggtgatg ctgatgagtg agcaggggtg aaaggccgat
     1321 caagacgaac cacgacatca ccgccgagcg cacgaacccg cgcaaatgac gcgcgtaggc
     1381 atcgaccaaa aaaagcagca acgccgcgtg cagtgacgga aaaaggttga acggcgcgtc
     1441 gagcccgcga aaccaatcga agaggaagcc gagccatccg tctgcgtgcg gtcgcggaaa
     1501 cgcgtagcgc aaggggacga acaagaaaaa acaaccggcc acgacgatgg cgacggacac
     1561 gcgccagacg aaaacccgca attcccgatc ggtccgaaaa agaaagggcg ccgcgatgaa
     1621 aaacagatcg atcgagagat aagggagaat catgaaggga acgaacggga tcgcgcgttc
     1681 ccaccgaaag tagaggacgc cgacatcgct gcgtcgcgcc gtgatccaga tgcagctccc
     1741 gtaaaccacc agaaagagga ccgacaaccc cacggaagcc gcgaacgccc tgatgcgcga
     1801 aactgagacg ggcgctttca ggtgagcgcg cgccgcgcga gcgtgacgct gaacattccc
     1861 caccgatcaa tttccatcgc gcatttctcg aagccggccg cgcgcaccag ttcgtcgatc
     1921 tccgccgtgg tgcggcgccg catgatccat cgttcccccg ctcgattgcg caggacacgg
     1981 gcgatgaact ccaactgcgg atgccagggc tgattggtat aaatcaagta gccgccgggc
     2041 tcgagcacgg cggccaatcc gcctaacgac tcgaggacag tgtcattgga tgggaacaat
     2101 tcgtaaagcc cggaaataat tccgatcgtc gcgcgcggtc tgagcgcggc gagggccgcg
     2161 cggtcgaagg cgtccccttg ctcgacggcg acattttcga ggccaaactc cgccgccagg
     2221 gcgcgcgccg cctcgatatt ctccagctcg ttgtcgcgca gggttgcgct gatcggtatc
     2281 tcccgcaggg cgcgcatcgt ctccagcaca taacgaccgg cgccggccgc gatatcgaga
     2341 atgcggaccg cccgcccgtc ggcgtgcagt ttttcgatcg cctcgcacaa tgctttttcg
     2401 aggttcacct tgcgctgccg gatcccgcgc cagccgatgc tgttgagata gttcgagtcg
     2461 atgaactttc cgagccgcga tgaaccttgc gcccgatttt cgtaaacgta atcgagcgat
     2521 cgccccgaat cgaacccgct ctgccagcca agatcgatgc ctttgctgag acgcccgccg
     2581 atcttcagcg cggccttgat ggcgagaaat tgcggaccac cgcgcaactt cagtttttca
     2641 tattcctccc aggtgtggcc gtatttgtcg gccgcgcgga gcgagaccgc gagcggcgcc
     2701 tgcgcgaagc gctcgacgat gaattcgcgc aatcgctcga tcacctgcgc ccggtccttt
     2761 tcgtgaaaga tggaatgata catcgccgga aaaacgtgca tccgcttcag cggcgaggag
     2821 aggcggttga agaactcccg ttgcgcatcc agactaacga cccaatcccg gcccgcggcg
     2881 agcatgagcg tcgggacgtg gatcgccccg gcgtccgcca gcaggcgtcg cgcggtgtcg
     2941 tggaggtcga gcaatacatt gattgcgatc tggcgaaaaa taagcggatc ggccgcgtag
     3001 cgggcggcct cgatcggatc gtgcgtcaac atcttcgccc gcacgtagct cgtcacctgg
     3061 cccgcgccga cggtcctctg atgcaagcgc aacgccggga tggcgagcgg aacgtagagc
     3121 cgcacgtcga acgccgccgt cgccaaaatg agcgcgcgaa tcggcggcgc gtaatcgtgc
     3181 acccacgcgc cgaccgtgac cgccgcgagg ctgtgcgcga gcacgatcat gttttcgcac
     3241 gcgatgccgt gccgttcctc gatgtgccta acgaacgtcg ccacatcctt gatcacgtcg
     3301 gtcagatcgc gcgcggcgcc gcgtgccccg ggggagcgcc cgtgaccacg cgcgtcccag
     3361 gcaaagatcg cgacgtcttc gaggccaagc gcctcgaccg tttcctgcca gcgcccagag
     3421 tgctcgtggc cccgatggaa aaggaagagc gcctgctccg cccgcttccc tttcggcaac
     3481 cacgcccgat agaacagctc cgtgccgtcc cagctcgtca tcgtttgctc agccgcttcc
     3541 atcggcctct cccaatctcc tgaccgcacc ctgcagctcc gaggcgatcg cgcagagatc
     3601 gctcttctcg aggctgcacg ctgcatagtt gcgcggtgcg ccgattatca gccgcacttt
     3661 cgccggtcgc gggaaccgtc gccccttcgg ccaggcgcga aacgcgccct gcacctgaca
     3721 gggcagtacg ttcacggcgc gtcccgccag gagcgcgccg atgcccgatt tgaacggttg
     3781 ggtttcgccc gtcatcgacc tcgtcccttc cgggaaaatg atgagcacgg tcccgggttg
     3841 cgcgagcaat tgcgcgcaga tggcgagatt ctcccgcacc cgcatgcgcc gcccgaaggg
     3901 catcgcattc atcaccaccg cggcaaacca ggtgcgcggc aggccgcgaa aaaagtagtc
     3961 ctctgccgcg accggaaagg cgcgcggcag acggcgcaaa ggcagcgccg cgagcaggca
     4021 aagggtgtcg agatggctgg cgtggtttgc caccaggacg aacgattcgt cgttaggcaa
     4081 attttcccgg ccaatgatct cgaagcgaag gtagatgcgg agcaggccgc gaataaccat
     4141 cgcgagcagc gcgcgcaaac tgctcacgaa ccggtccggt tcgcgcggaa agcggcgcag
     4201 gcgctcgacc gccgttcgct cgaaaacgag cgcggacgca taaccccatc ttctcatcgc
     4261 aacggataat agtgtccggc catcgccatg aacagcggcg cgacgaaaat caggctgtcg
     4321 atgcgatcga gcacgccgcc gtggcccgga atggccgcgc cccaatcctt cgccgcgaga
     4381 tcacgcttga tcacgctgag cgaaaggtcg ccgagctgcc cgccgacgcc gacgatcagg
     4441 ccgacggcga gcaggtccgc cggcccgaag tgcgggaacg tgaaccacag cgcccacggc
     4501 ag
//
LOCUS       DATMNS010000256         3405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3783567, whole genome shotgun sequence.
ACCESSION   DATMNS010000256 DATMNS010000000
VERSION     DATMNS010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3783567"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(334..1494)
                     /gene="nifS"
                     /locus_tag="VNW28_09020"
     CDS             complement(334..1494)
                     /gene="nifS"
                     /locus_tag="VNW28_09020"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR03402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HXA10111.1"
                     /translation="MSAGSEVIYLDNNSSTRIDPEVLEEMMPFLTTHYGNPSGSHRFG
                     AQVKAATERAHQQVAALLGCQPNEVVFTSGGTESDNTALHSALQMSPERRHVVTTSVE
                     HNAVLNYCEAIVRRGCSVTIVEVDERGHLDLAELEAAIRPDTAVVSVMWANNETGDIF
                     PLARISEICRAKGVFFHTDAVQAVGKMPIDLTKLPVHFLSLSGHKLHACKGVGALYVS
                     RKSRFQPLLVGGPQEGGRRAGTDNVASIVGLGKAAELAVATLEEENTRVRALRDRFEA
                     TLLAELDDVSVNGDPGARLPNTSNLAFNGVDAQAILLQLDREGVCCSLGSSCTTGAVQ
                     PSHVLRAMHLTNERARSSLRFSFGRFNTEAELDKVVEIIPRAIRKLRQLAVS"
     gene            complement(1532..1873)
                     /locus_tag="VNW28_09025"
     CDS             complement(1532..1873)
                     /locus_tag="VNW28_09025"
                     /inference="COORDINATES: protein motif:HMM:NF019214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HXA10112.1"
                     /translation="MHTFQFLADGNFLGLSLNGWKFVGWAGNIVFGARFIIQWIASER
                     ARKSVIPAGFWECSAAGSLLLLIYFAFYRRDSVGVLSALFPLPIYLRNLYFRYSHHHA
                     THPGNEPRAEE"
     gene            1964..3061
                     /gene="hisC"
                     /locus_tag="VNW28_09030"
     CDS             1964..3061
                     /gene="hisC"
                     /locus_tag="VNW28_09030"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXA10113.1"
                     /translation="MDSVWDQANPHLRDLALYQPGKPIEETARESGLRVDQIVKLASN
                     ENPLGPSPRARAAMQAALAGANLYPDGGGFYLCAALADKQKVAPENIILGNGSNEIIE
                     FIGHAFLRPGAELVTSENAFIAYRLVASLFGATAIEVPDREYRFDLEAVRQAITPRTR
                     LVFIANPNNPTGTLVRQEAIDGFMAGLPNDVVVVFDEAYFEYLDDAPDTLRLVREGRN
                     AIVLRTFSKIHALANLRIGYGMARPELIRVLQKAREPFNTNGVAQAGALAALTDEAHQ
                     AESKRITDEGRRYLEGEFTAMGLAFVPSVANFVLVKVGDGKAVFKALLRHGVIVRALS
                     GYKFPEWVRITVGTMEENRTCIAALREVLAA"
     gene            3058..>3405
                     /locus_tag="VNW28_09035"
     CDS             3058..>3405
                     /locus_tag="VNW28_09035"
                     /inference="COORDINATES: protein motif:HMM:NF021882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HXA10114.1"
                     /translation="MMPDETTATIAAVSTPLGEGAIALVRLSGPQAIAVADQVFRSKQ
                     KPSAAGSHLQQFGEIVAEEERVIDQVMLSVHRAPASYTGEDVVEISCHGGVLVTARVW
                     EACLQAGARAARPG"
BASE COUNT          676 a         1047 c         1084 g          598 t
ORIGIN      
        1 gttccacaag ggcggagagg cgggattgag cagtgctggt ggaagccacc gacgagcatc
       61 gattctgccg gccgaaagcg caaacttcgg acgaataagt gcctaacgac gagggagaaa
      121 acaatgcgac cgcccgcgca cgcccgatac tcaactgcgc tttcgcaccg tcgcgcggat
      181 agcaaattgc gaaagagtgc gtcgcgaatt ccacgccagc ccgttcactt tgaactggaa
      241 gtaggccttg gcagacgttt cgctctgcga agcgcggaaa ggggcgccgg tctggatgcg
      301 atagcctcgc tcgcgcctcc caaaaggggg cgtctaggac acggccagct ggcgcagctt
      361 gcgaatcgcg cgcgggatga tctcgaccac cttgtccagc tccgcctcgg tgttgaagcg
      421 gccaaaggag aatcgaaggc tgctccgggc ccgctcgttc gtcaggtgca tcgcccgcag
      481 gacgtgcgac ggctgcaccg cgccggtggt gcaggatgag cctaacgaac aacagacgcc
      541 ttcccggtcg agctggagca gaatggcctg cgcatcgacg ccattgaaag ccaggttcga
      601 ggtgttcggc agccgcgcgc ctgggtcgcc gttgaccgac acgtcgtcga gctcggcgag
      661 caaggtcgcc tcaaagcggt cgcgcaatgc gcggacccgc gtgttttctt cctcgagggt
      721 tgcgaccgcc agttccgccg ctttcccgag cccgacgatc gaagcaacgt tatcggtccc
      781 ggcgcgccgg ccgccctcct gcgggccgcc gacgaggagc ggctggaagc gcgacttccg
      841 actgacatag agcgcgccga cacccttgca cgcatgcaat ttatggcccg agagggagag
      901 gaaatggacc ggcaatttcg tcaggtcgat tggcattttg ccgaccgcct gcaccgcatc
      961 ggtgtgaaag aagacgcctt tcgcgcggca aatttcggaa atcctggcga gcggaaaaat
     1021 gtcaccggtt tcattgttcg cccacatgac cgagacgacc gcggtgtcgg gccggatggc
     1081 ggcttccagc tccgccaggt cgagatgacc ccgctcgtcg acctcgacga tcgtcacgga
     1141 gcaaccgcgc cgaacgatcg cctcgcagta gttcaggacg gcattgtgct cgaccgaggt
     1201 cgtcaccaca tggcgtcgtt cgggcgacat ctggagcgcg gagtggagcg ccgtgttgtc
     1261 cgactccgtc ccgccgctcg tgaaaacgac ttcgttgggc tggcagccga gcaaagccgc
     1321 gacctgttgg tgggcccgct cggtcgccgc cttcacctgc gcgccaaagc gatgtgagcc
     1381 cgacggattc ccgtagtgcg tggtcaggaa cggcatcatt tcctccagca cttccggatc
     1441 aatccgggtg ctcgagttat tatcgagata gattacttca cttccggcgc tcatggccaa
     1501 aggttaccga gtttctccga cgtcgcacca tctactcttc cgcccgcggc tcattcccgg
     1561 gatgggtggc gtggtgatgc gaataacgaa aatacaggtt ccggagatag atcggcaatg
     1621 gaaaaagcgc actcaagacg ccgactgaat cgcggcggta aaacgcgaag taaatgagca
     1681 aaaggagaga cccggccgcg ctgcactccc aaaatccggc gggaatgacg ctcttgcgcg
     1741 cgcgctcgga ggcaatccac tggatgataa agcgggcgcc gaaaacgatg ttgcccgccc
     1801 aaccgacaaa tttccagccg ttgagcgaaa ggccgagaaa gtttccatcg gccaggaatt
     1861 ggaaggtgtg catagagggg aaggttccct caggatggcg cgggctgaaa cagctatttc
     1921 cctggactgt tcaggcttgg aatttcgctt aacgttgccg gcaatggact ccgtctggga
     1981 ccaggccaat ccacacctgc gcgacctcgc gctttaccaa ccgggcaaac cgatcgagga
     2041 aaccgcgcgc gagtcaggtc tgcgagtcga ccagatcgtc aagctggcct cgaacgaaaa
     2101 cccgctcggc ccctcgcccc gcgcgcgggc cgccatgcag gctgcgctcg ccggcgcgaa
     2161 cctttacccc gatggcggcg gcttttatct gtgcgcggcg ctggcggata aacagaaggt
     2221 cgcgccggaa aatatcatcc tcgggaacgg gtcgaacgaa atcatcgagt ttatcggaca
     2281 cgcttttctc cggccggggg cagagctggt gacctccgaa aacgccttca tcgcctaccg
     2341 gctggtggcg tccctcttcg gcgccaccgc gatcgaagtc ccagaccgcg aatatcgctt
     2401 cgacctcgag gccgtccggc aggcgatcac cccgcgcacc cggctggtct tcatcgccaa
     2461 tccaaacaac ccgacgggaa cactcgttag gcaagaggcg attgacggtt tcatggccgg
     2521 cctgcctaac gacgtcgtgg ttgtcttcga tgaggcttat tttgagtatc tggacgacgc
     2581 gcccgatacc ctgcggttgg tgcgcgaagg tcgcaacgcc atcgtcctgc ggacattttc
     2641 caaaatccac gcgctcgcca atctccgaat cggctacggc atggcgcgcc cggaattgat
     2701 ccgggtgctg caaaaagccc gcgagccatt taacaccaat ggcgtcgccc aggccggggc
     2761 cctggcggcg ctgaccgatg aggcacatca ggcggaatcg aaacggatca cggacgaagg
     2821 ccggcgctat ctggagggag aattcaccgc catggggctg gctttcgtcc ccagtgtcgc
     2881 caatttcgtg ctcgtcaaag tgggcgatgg caaagcggtt ttcaaagcgc tcctccgcca
     2941 cggcgtgatc gtgcgcgcgt tgagcggtta caaatttccc gagtgggtgc gcatcaccgt
     3001 cggaacgatg gaagagaatc gcacatgcat cgccgcgctg cgcgaggtgc tggcggcatg
     3061 atgccagatg agacaacggc gaccatcgcc gcggtctcga cgccgctggg agaaggcgcc
     3121 attgcgctcg tcaggttgtc cggcccgcag gcgatcgcgg tcgcggatca ggtttttcgc
     3181 agcaaacaaa agccgtcggc ggccggatcg catttgcagc aattcggcga gatcgtcgcg
     3241 gaggaggagc gggttatcga tcaggtgatg ctctcggtcc atcgcgcgcc ggcgagttac
     3301 accggcgaag acgtggtcga gatcagttgc cacggcggcg tgctggtgac ggcgcgggtt
     3361 tgggaagcct gccttcaggc aggcgcgcgc gcggcccggc cgggg
//
LOCUS       DATMNS010000257         5142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1306217, whole genome shotgun sequence.
ACCESSION   DATMNS010000257 DATMNS010000000
VERSION     DATMNS010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306217"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(508..1983)
                     /locus_tag="VNW28_09040"
     CDS             complement(508..1983)
                     /locus_tag="VNW28_09040"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HXA10115.1"
                     /translation="MPVVPFHRFSLRTKVAFAMAIVAALVVAAILGTNYHFRRAQLLQ
                     EFQAFVRGAAGTTALALHGEEINSIRAPNDATSPAFQSARAILDQSRRINGLAENEMY
                     ILRPVGATSPLETEFVVMLQKKTFIGTSYILLEANRDQFLKAWKTRLPTSSDVYEDEN
                     GTWISGYGPILNQAGEPVAIVEADARISRFVARQREELLLALAIGCGAFCVAMIPGLL
                     LARNITRGLNKLSSGIRRFQAGENDVQVSVTTGDELQHLGDVFNEMIISLGEKLALLP
                     YVSRFTAEAVRRSRKDPQWLAGMEQEVIVLFADLRGFTSWCETREASFLVRELNRLLA
                     VQADAVVSAGGDVDKFIGDAVMAVFLGGEGSAEKVFACARELVAAIRREIEAEGWPLA
                     LGVGIHRGRAVVGSIGSDTRRDFTAIGHTVNLASRLCDRAGKWQILVSEPFYKMLPAE
                     KQAEFERTEPMEFKHVSQMVPTYRFTVTVAGGVVPGSVPQL"
     gene            complement(2095..2670)
                     /locus_tag="VNW28_09045"
     CDS             complement(2095..2670)
                     /locus_tag="VNW28_09045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXA10116.1"
                     /translation="MESINRPVFLPVILGTARQGRISEHAARFVFEEVSQRPDMTTEL
                     IDIRNLRLSIADAGEALKDMQFSATVSRADGLILVVPEYNHSFPGLLKHVLDSNLKEY
                     IHKAVGVCGVSAGPFGGARMIQSLLPVLRELGLVTIFSDVYFGTAGKLFDSATGNITD
                     PAYVGRLKKFVDELVWMARALRFARENIPEL"
     gene            2990..>5142
                     /locus_tag="VNW28_09050"
     CDS             2990..>5142
                     /locus_tag="VNW28_09050"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA10117.1"
                     /translation="MIAQYSRLFRWHVLRYLAQHPLLATLNVATVALGVALYLAIQIA
                     NQSANHAFAASIDVVAGKAQLEVNAPAGDLPDELLPKIARQSGVAAATPIVRGFVTLP
                     RFPGEYLDLLGIDVFTNEPFRTFQLTDFQTQTFDLQQWLRGPRTIALSEEFARRHHLR
                     AGDEIAVQNNGATKYLKIGFLLRRDDSGADLDSHFAAIDIGWAQEFLGRSGTLDSVSL
                     RLTNNADSKAVAGELRKIVPADVTVAPPAQRGEEVDKMLSGFQLNLQAMSLVSLLVGM
                     FLVYNTIEASVVRRRNEIGILRSLGATRSEVRWLFLGEAIVLGLVGIALGLVGGYFLA
                     RALVGTVAETISSLYVLISVQNISIAPWIWMSAVLLGLASVFVAAWLPAQAAATTDPV
                     QALHGGARIERSVHLSRRWIFGGLFSLLVSVLLSVLALQTGPPWLGFGAAFFVLAGFS
                     LMAPELIARFSLAVRSDWQRRIEPLLAAQNLGRALLRNSITIASLAAAVAMTVGVAVM
                     VFSLRQTVTSWIDQTLIADLFIGPAANEIAGPTSFMPPEAIRFLEADPAVEAVDTFRE
                     VDLPFRDQTISVGVVRGTNRRSLRFLHGKNDQIMPRFYSEQCVLVSESFVRRYQVTDG
                     GTLKLPTPSGMVAFPIAGVFYDYASDQGTVFLSERNFQRLWHDDRVNSVAVYLKKNAS
                     PAAIADTFRQRFSHRGEFSIYSNRSLRTRIFEIFD"
BASE COUNT          962 a         1623 c         1550 g         1007 t
ORIGIN      
        1 gtggaccaag cgcgaagttt attgcgtgaa atatcatcac cacaacaatg cgcgttgcat
       61 tttcaactgt cgtttcccga actggcgaag cgcgaactgt agccgggtcg atgacccagc
      121 ccgcggttcc tcgaggctgt catgaacttg cgggtttgcg cggccagcaa aatccacgtc
      181 ctgtagccgt caccctgtgt gcgacgcggg tgcgatggtc agcccccttg ccgcgcgcat
      241 accccatgct tcgcgcagcg aagcggctac aactgcgcgg gcgcatgctt tcccggcgcg
      301 ttccacccct gtagccgtcg ccctgtaggc gacgcgagta cgacgcgcca accgccttgc
      361 cacacacacg taccggcgct tcgcacagcg aagcggccac agctcgtggc ccggccgtga
      421 agtccgggac acgcacactt aacgctctgt tgcaaaatgc atgacggcct ctaaacacgt
      481 gcacgctacc gggatcaccg atcccgccta caactgtggc acactgccgg gaacaacgcc
      541 cccggctaca gtgacggtga agcgatacgt cggcaccatc tgagacacgt gcttgaattc
      601 catcggctcg gtccgttcga actcggcctg cttttccgcc gggagcattt tgtaaaatgg
      661 ctcgctcacc aggatctgcc atttcccggc ccgatcgcag aggcgcgagg ccaggttcac
      721 cgtgtggccg atggcggtaa agtcgcgccg ggtatcggaa ccgatcgacc caaccactgc
      781 gcggccccga tggatgccga cgccaagggc cagcggccag ccttccgcct cgatctcccg
      841 ccgaatggct gcgaccaatt cccgggcgca ggcgaacact ttctcggcgg aaccttcgcc
      901 gcccagaaaa accgccatca ccgcgtcgcc aatgaatttg tccacgtcgc cgccggccga
      961 gacgaccgcg tccgcctgga ccgcaaggag ccggttgagc tcgcgcacga ggaaggaggc
     1021 ttcgcgcgtc tcgcaccagc tggtgaagcc gcgcagatcg gcaaagagca cgatcacctc
     1081 ctgttccatg ccggcgagcc attgcggatc cttcctgctc cggcgcaccg cctccgcagt
     1141 gaagcgcgag acatagggca ggagggcgag cttttcgcct aacgagataa tcatttcgtt
     1201 gaagacgtcg cccaggtgtt gcagttcgtc gccggtcgtc acgctcacct ggacgtcatt
     1261 ttccccggct tggaatcgcc gaatgcccga agaaagtttg tttagtccgc gggtgatgtt
     1321 gcgggcgagg agcaggcccg gaatcatcgc cacgcaaaac gcgccgcagc cgatcgccag
     1381 cgcgagcaaa agttcttccc gttggcgcgc gacgaatcgg ctgattcgcg cgtccgcctc
     1441 gacgatcgca acgggctccc cggcctgatt caggatcggt ccgtagccgc tgatccaggt
     1501 gccattttcg tcctcataca cgtcgctgct cgtcggcagg cgtgttttcc aagccttgag
     1561 gaattggtcg cgattggcct ccagcagaat gtaggaagtg ccaatgaacg tcttcttctg
     1621 caacatcacg acgaattccg tttccaacgg ggaggtcgca cccaccgggc gcaggatgta
     1681 catttcgttt tccgcaagac cgttgatccg gcgggattga tcgagaattg ccctcgcgct
     1741 ttggaaggcc ggcgaggtag cgtcgttagg cgcacggatc gagttgatct cctcgccatg
     1801 cagagcgagc gcggtcgtcc cggccgcacc acggacaaag gcctggaatt cctgcaggag
     1861 ttgcgcgcgc cggaaatgat agttggtgcc gagaattgct gccacgacca gcgccgccac
     1921 gatcgccatg gcgaaggcga ccttggtgcg aagcgaaaaa cgatgaaatg gaacaacggg
     1981 catgtccgaa gtgcggcggg cgcgtcaacg atcacgcgtc tccggattct aagtcgagca
     2041 ggaacgtcgc atcctgacaa gcgcgccgtt gtcgccgagg accagcgctc ccggctacaa
     2101 ctccggaata ttttcccggg cgaaacgcag cgcacgcgcc atccagacga gttcgtcgac
     2161 gaatttcttg agtcggccca cgtaggccgg gtcggtgata tttcccgtcg ccgaatcgaa
     2221 caacttgccg gccgtcccga agtagacgtc cgagaagatc gtgaccaggc ccaactcgcg
     2281 cagaaccggc aacaaacttt gaatcatgcg ggcgccgccg aagggtccgg ccgagacgcc
     2341 gcagacgccg acggctttgt gaatgtattc cttcaagttg ctgtcgagca catgcttgag
     2401 caggccggga aagctgtggt tgtactcggg cacgacaaga atgagtccat ccgcgcggct
     2461 gaccgtcgcg gaaaactgca tgtccttgag cgcctcgccc gcgtccgcga tcgagagccg
     2521 gaggttgcgg atgtcgatca attcggtcgt catgtcgggc cgctgggaaa cctcttcgaa
     2581 gacaaaacgg gcggcgtgtt cgctgatgcg accctgacgc gcggtgccga gaataacggg
     2641 aagaaagacg gggcggttga tcgattccat aagggcttgg tcggcgccga aggcagaaac
     2701 ttacacgctg gcgttgtaac cgggaccggt gctccaggca atcactcaaa aaatctgtcc
     2761 tcccatcgtg gaacggccgg gatcaacgac cccggctaaa aactaacgaa ggacgccaag
     2821 ccgcgcagat tcgattgcgg gtccgctccg cgccgggctc gtcgtagccg gacgaaacag
     2881 tgctgcgccc accacgcatg acgcagccct gggcgtggaa gtgcaaggcc tgcgggcagg
     2941 ctgacgcatt cgccgccggc tattttctca gcatcttctc aagcaccact tgatcgcgca
     3001 atactctcgc ctcttccgct ggcacgttct ccgttacctg gcgcagcatc cgctcctcgc
     3061 aacgctcaac gtcgcgaccg tcgcgctcgg ggtcgcgctc tacctcgcga tccagatcgc
     3121 caaccagagc gccaatcacg ctttcgccgc gagcatcgac gtggtggcgg gcaaagcgca
     3181 actcgaagtg aacgcacccg ccggggatct acccgacgaa ctactcccga agatcgcgcg
     3241 gcagtcgggc gtcgcggcgg cgacgcccat cgtgagggga tttgttactc tgcccaggtt
     3301 tcccggtgaa tacctcgacc tgctcggaat cgacgtcttc acgaacgaac cgtttcgcac
     3361 tttccagctg acggattttc aaacgcaaac cttcgacctg cagcaatggc tgcgcggccc
     3421 gcggacgatc gccctgagcg aagagttcgc gcggcggcac cacctgcgcg cgggcgatga
     3481 gatcgcggtg cagaacaatg gggcaacgaa atacctgaag atcggattcc ttctccgccg
     3541 cgacgattcc ggggcagacc tcgattcgca ttttgcggcg atcgacatcg gctgggccca
     3601 ggaatttctg ggccggagcg gaacgctcga ctcggtttcg cttcgcctaa cgaacaacgc
     3661 ggattcgaaa gcagtcgccg gcgagctgcg caaaatcgtc ccggcggatg tcaccgtggc
     3721 gccgccggcg cagcgcggcg aggaggtgga caaaatgctt tccggtttcc agctcaactt
     3781 gcaggcgatg agcctcgtct ccctccttgt ggggatgttc ctggtctaca atacgatcga
     3841 ggcctcggtc gtgcgacgac ggaacgaaat cgggatttta cgttcgttag gcgcgacccg
     3901 ctccgaggtc cgctggctct tcctcggcga agcgatcgtc cttggactgg tcgggatcgc
     3961 gcttggattg gtcggtggat atttccttgc tcgtgcgctc gtcgggacgg tcgcggagac
     4021 gatttcctca ctctatgtcc tgatcagcgt acaaaacatc tcgattgcac cctggatttg
     4081 gatgagcgcc gtcctgcttg gattggcctc ggtcttcgtc gccgcctggc tgccggcgca
     4141 agccgcggcc acgaccgatc cggtccaggc cctgcatggc ggcgcccgaa tcgagcgttc
     4201 ggtgcacctg tctcggcgct ggatatttgg cgggctgttt tcgttgctcg tgtcggtcct
     4261 gctttctgtc ctcgctcttc agacgggtcc gccctggcta ggtttcggag cggcgttttt
     4321 tgtcctcgcc gggttttccc tgatggctcc tgaattgatc gcgcgcttca gcctggccgt
     4381 gcgatccgac tggcagcggc ggatcgagcc gctcctcgcc gcccagaatc tcggtcgcgc
     4441 gctgctccgc aattccatca ccattgcgtc gctcgcggcg gccgtggcga tgaccgtcgg
     4501 cgtggcggtg atggtttttt cattgcgcca gaccgtcacc tcctggatcg atcaaacctt
     4561 gattgccgac ctcttcattg gtccggcggc gaatgagatc gccggtccaa catcgttcat
     4621 gccaccggaa gcgattcgtt ttctcgaagc ggatccggcg gtggaagcgg tcgatacttt
     4681 ccgcgaagtg gatctgccgt ttcgcgatca gacgatttcg gtcggggtgg tgcgggggac
     4741 caaccggcgc agtcttcgtt tcttgcacgg gaaaaacgac caaatcatgc cccgttttta
     4801 cagcgagcag tgtgtgctcg tctcggaaag cttcgtccgc cgctatcagg tgacggacgg
     4861 cgggacgctg aagttgccga cgccgtccgg catggtggcg ttccccatcg ccggagtctt
     4921 ttacgattac gccagtgatc agggcaccgt cttcctgagc gaaaggaatt tccaaagact
     4981 ctggcatgac gatcgggtca acagcgtggc ggtctacctg aaaaagaacg cttcgcccgc
     5041 ggcgatcgcg gacacatttc gccagcgttt cagccatcgc ggcgagtttt cgatctactc
     5101 caatcggtcg ttgcgcacgc gaattttcga aatcttcgat ca
//
LOCUS       DATMNS010000258         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1306465, whole genome shotgun sequence.
ACCESSION   DATMNS010000258 DATMNS010000000
VERSION     DATMNS010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306465"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1122
                     /locus_tag="VNW28_09055"
     CDS             136..1122
                     /locus_tag="VNW28_09055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXA10118.1"
                     /translation="MNSSTPLLSFCIPTRNRTGFLREFLESVAAQITPEVEVVISDDG
                     STDDTPAVIECFRTRIPQLFARRVDPPLRYDRNLLHVVSLARGKYCWLFGDDDRLEPG
                     GLAAVLEVLKVEPDPAGLTTDRVSYDSALANKLPVRALKQQQSALFQNAEEAFLRLLD
                     RLGFLSCQIVNRALWNEIVSRENLEPYFTGYVQLYVIARLLVQAPNWRFLAQPCVAFR
                     ADNDSFRALGQLGRLKMDVCGYETIVGDVFGRDSRLYHEAMAEVARTHARHHIVTAKR
                     AGAPSSFFWQALKLCVQHYGRYASFWLQTFPVLIMPRSIMLLLRSWYQRLRP"
     gene            1126..1938
                     /locus_tag="VNW28_09060"
     CDS             1126..1938
                     /locus_tag="VNW28_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10119.1"
                     /translation="MDTAIVGVCCDPRQRRILEVTFPSWREYGKRHGLPVIILERSHA
                     GQDFYWNKHLLYRVPELRAARRLLFLDNDVFVNPNARALLEEWDSPLIGATAESTQAG
                     WSPEFIARYYAEYAVDQSSPVADLQIINTGVLVIPHEQAEFLEGVYQRWRQRKNAREK
                     SAKSKDPFVHAADQPHVSYALQAEHRCKDFGPRSNTLWWHWYRQHVSSRQMPFLLRSK
                     AAALTRDRIPRALWRALFRRERATFARGLASADFLHVAGSKSSLFLGENYGQ"
     gene            1944..2636
                     /locus_tag="VNW28_09065"
     CDS             1944..2636
                     /locus_tag="VNW28_09065"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXA10120.1"
                     /translation="MWLTRLRVQLKSVLPRESLLYRLGHRGYQLIVRLLDRFGRHNAP
                     REITENSIFIREEELPMCSIFPKEILDLTLELFHPRSVLDVGCGTGISLDYFKSKGIS
                     VRGLEGSALAISKARNAPLILQHDLNEPINLEEGFDLVWCFEVAEHIHPSYVGALLAT
                     LTNHAERIVLSAARPGQGGLGHLNEQPPDYWIGRMQERGFALSHETGKYRELPDHFAE
                     NILIFERIDGPR"
     gene            2623..3510
                     /locus_tag="VNW28_09070"
     CDS             2623..3510
                     /locus_tag="VNW28_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10121.1"
                     /translation="MGLDSDGTRFLFAAQAGGVSFRNTAMLGRQNFFPAVGPLQQLLD
                     LRKPGLSAVQFLADSHGYAEHLFRFLGAEEVVSLDNSDYEGAAIVTDLNAPIAATLKN
                     RFTALLDGGCLEHVFNLPQAIKNSMEMLRVGGHFLAITPANNFCGHGFYQFSPELFFR
                     VFSEPNGFAMRAVLTKEKERWFRVIDPEVFRGRVEIQNNRPTYLFVLAKKLETRDVFV
                     QSPQQSDYARQWEGKAKKNMPLPAAVPSFSAKMRAILPTKWKEALRPFAANISIRHRY
                     SCYQPIDEVKLLRGEFTID"
     gene            3688..4521
                     /locus_tag="VNW28_09075"
     CDS             3688..4521
                     /locus_tag="VNW28_09075"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXA10122.1"
                     /translation="MTAKFQEFESLTHCPGCGSDCVGKALEPDVGQCCNCRLYFRNPR
                     PTQAEIARSYNTGGTFDAWQAEEPARAVMWERRLAIVRHFAGGGRLLDVGTGDGRFLA
                     TARPAGYEVAGTEVSEAGASYARERGFDVRLGQITELDLPSESFDLATIWHVLEHVPD
                     PGAVLRKVHSLLRPGGILVVAVPNEENFFIRRRLGAAKSSPFDPLKFGGEIHLTYFRP
                     GTLRSTLRAAAFEVREFGVDDVYHVRDLKMRLKLPFQRALARMLNWHFAVAMYAVCQR
                     K"
BASE COUNT          906 a         1474 c         1359 g         1019 t
ORIGIN      
        1 cactcggcgc gcgatcatga ccaccgcgcc gcccgaattg gtcttcgcca cgtgtcgctg
       61 tggcagcttt cgccgcctcg ctcagcgcag gcagcggcag cctaatggcc aggctctccg
      121 cttaacctcg cgcccgtgaa ctcttccaca ccgcttctct ctttctgcat cccgacgcgc
      181 aaccggaccg gatttctccg tgaatttctg gagagtgtcg ccgcgcagat cacgcccgag
      241 gtggaggtcg tcatttccga tgacggatcg accgatgaca cacccgcggt gattgaatgt
      301 ttccgcacgc gcattccgca gctcttcgcg cggcgggtcg atcctccgct gcgctacgac
      361 cgcaacctgc tccacgtcgt ctcgctggcg cgcggaaaat attgctggct gtttggggac
      421 gacgaccgcc tcgagcccgg cggacttgcc gcggttctgg aggtgctgaa ggtcgaacca
      481 gacccggctg gcctaacgac cgaccgtgtt tcctacgaca gcgcgctggc gaacaagctc
      541 ccggtgcgcg ccttgaagca gcaacagagc gcgcttttcc aaaacgccga ggaagctttc
      601 ctgcgcttgc tcgaccgcct ggggtttctt tcctgccaga tcgtcaaccg cgctctctgg
      661 aacgagatcg ttagccgaga aaacctcgag ccctatttca ccggctacgt tcagctttac
      721 gtcatcgcgc gcctgctcgt gcaggctcca aactggcggt tcctcgccca gccctgcgtc
      781 gcctttcgcg ccgacaacga ttccttccgc gccctcgggc aactgggtcg cttaaaaatg
      841 gatgtctgcg gctacgagac gatcgtgggc gacgtcttcg ggcgagactc ccggctctat
      901 cacgaagcca tggccgaggt cgcgcgcact catgcccgac accacatcgt aacggcaaag
      961 cgcgccgggg cgccgtcctc gtttttttgg caggcgctca agctttgcgt gcaacactac
     1021 gggcgctacg ccagcttttg gctgcaaact tttcctgtcc tcatcatgcc gcgatcgatc
     1081 atgctcttgc tgcgcagctg gtaccaacgt ttacgcccct aaccgatgga cacggccatc
     1141 gtgggagtct gctgcgatcc gcggcaacgg cgcattctcg aagtcacttt tccgtcctgg
     1201 cgcgagtacg gaaaacggca cggcttgccc gtgattattc ttgaacgctc gcatgcaggc
     1261 caagatttct attggaacaa acacctcctc tatcgtgtgc cggagctgcg cgcggcgcgg
     1321 cgactcctct ttctcgataa cgacgtcttc gtcaatccaa acgcccgggc tctgctcgaa
     1381 gaatgggatt cgccgttgat cggcgcgacc gcggagagca cgcaggccgg ctggtcgccg
     1441 gaattcatcg cacgctatta cgctgaatat gcggtcgatc agtcttcgcc ggtggcagac
     1501 ctgcaaatca tcaacaccgg cgtcctcgta atcccgcatg aacaggccga atttctcgag
     1561 ggcgtctacc agcgctggcg acagcgaaag aacgctcggg aaaagtcggc gaagtcgaaa
     1621 gacccgttcg ttcacgccgc cgatcagccg cacgtctcct acgcccttca ggcggaacac
     1681 cgctgcaaag acttcggccc gcgctccaac acgctctggt ggcattggta tcgtcaacat
     1741 gttagttccc ggcaaatgcc ctttcttctc cgttccaagg cggctgccct gacgcgcgac
     1801 cggatcccga gagcgctttg gcgcgctctc tttcgtcggg agcgcgccac gttcgctcgc
     1861 gggctggcga gcgccgattt cctgcatgtc gccggcagca aatcctcgct ttttctggga
     1921 gagaattacg gccagtgaac gcgatgtggc tcacgcggct gcgagtgcag ctaaagagtg
     1981 tgcttcccag agaatcgctg ctctatcgtc ttggccatcg tggctatcaa ctcatcgtta
     2041 ggctgttgga tcggttcgga cgacacaacg caccgcgtga gatcacggaa aactcgattt
     2101 ttatccgcga agaagagctg ccgatgtgct cgattttccc gaaggaaatc ctcgacttga
     2161 cgctcgaact ttttcatccg cggtcagtcc tggatgtcgg ctgcgggacc gggatcagcc
     2221 tcgactactt caagagcaaa ggaatttccg tccggggttt ggaaggttcg gcgttggcca
     2281 tctccaaagc ccgtaacgcc ccgctgatcc tgcaacatga cttgaacgaa cccatcaatc
     2341 tcgaagaagg ctttgatctg gtttggtgtt tcgaggtcgc cgagcacatc cacccgagtt
     2401 acgtcggagc attgctcgcc accctgacaa atcacgccga acgcatcgtt ctctccgccg
     2461 cgcggcccgg gcaaggcggc ctcggtcact tgaacgagca accgcccgat tactggatcg
     2521 ggcgaatgca agagcgcggc ttcgcgctca gccacgaaac cggaaaatat cgcgaactac
     2581 cggaccactt cgcagaaaac atcctgattt ttgaaaggat cgatgggcct cgatagcgac
     2641 ggaaccaggt ttctcttcgc cgctcaggca ggcggggttt cttttcgcaa caccgccatg
     2701 ttggggcggc agaatttctt tcccgcggtg ggtccgctgc aacaacttct cgatctgcgc
     2761 aaaccgggat tatcagcggt ccaattcctt gccgattccc atggctacgc agaacacctg
     2821 ttcaggtttc tcggcgctga ggaggtcgtt tcgctcgata actccgatta cgaaggcgct
     2881 gcgatcgtca cggatttgaa tgcgccgatc gccgcgacgc tgaaaaaccg gttcaccgca
     2941 ctcctggacg gcggttgtct tgaacatgtt ttcaatctcc cccaggcgat caagaacagc
     3001 atggagatgt tgcgcgttgg cggccacttc ctcgccatta ctccagcaaa taatttttgc
     3061 ggtcatggct tctaccaatt cagtcccgaa ctcttctttc gtgttttttc ggagccaaac
     3121 ggctttgcga tgcgcgctgt cctgaccaag gaaaaggaac gctggtttcg cgtcatcgat
     3181 ccggaggttt tccgcggccg ggtggaaata caaaacaacc ggcccaccta tctgtttgtt
     3241 ctggcgaaga aactcgaaac tcgtgacgtc tttgtgcaat ctccgcagca aagcgattac
     3301 gcgcggcagt gggaggggaa ggcgaagaag aacatgccgc tgcccgccgc agtgccatct
     3361 ttttcggcga agatgcgggc aattcttccc acgaaatgga aggaagccct gcgacctttt
     3421 gccgccaaca tctcgatccg gcaccggtat tcgtgttatc aaccaatcga cgaggtaaag
     3481 ctcctccgag gcgaattcac aatcgattga ttccagggaa atgaactcgt tttatctcgt
     3541 taggcaaaaa ttggcgatag ctgccaccac cgtccgagcc tgttctaatt tgctgatcaa
     3601 cgcgcgaacg agactcgctg tcggcgctca tatccgagcg tctctcctgc ctcgccgggc
     3661 acggccgagg aaacgaggaa aacagacgtg actgcgaaat tccaagagtt cgaaagcctg
     3721 acacattgcc cgggctgcgg cagcgactgt gtcgggaaag cgctcgagcc cgacgtcgga
     3781 caatgctgca actgccggct ctactttcgc aatccgcgcc cgacgcaggc ggagattgcc
     3841 cgctcttaca atacgggcgg aactttcgac gcgtggcagg cagaggaacc ggcgcgcgcc
     3901 gtgatgtggg aacgacgttt ggcgatcgtt cgacattttg ccggcggcgg ccggctgctc
     3961 gatgtcggaa cgggcgatgg ccggtttctc gccacggcgc gaccagccgg ttacgaggtc
     4021 gcggggacgg aagtgtcgga agcgggtgcc agttacgccc gggagcgcgg cttcgatgtt
     4081 cgtctcgggc agatcaccga acttgacttg ccctcggaga gtttcgatct ggcgacgatc
     4141 tggcacgtgc tcgagcatgt acccgatccg ggcgccgtcc tgcgcaaagt gcattcgctg
     4201 ctgcgtcccg gtggcatcct ggtggtggcc gtgcctaacg aggaaaattt tttcattcgg
     4261 cggcgcctgg gcgcggcgaa aagcagccct ttcgatccgc tcaagttcgg cggggagatc
     4321 catctcactt acttccggcc ggggacgttg cgctccacgc ttcgcgctgc ggcgttcgag
     4381 gtgcgggagt ttggggtgga cgatgtttat cacgtgcgcg acctgaagat gcgattgaag
     4441 ctaccatttc aacgggccct ggcgcggatg ttgaactggc attttgcggt ggcgatgtat
     4501 gcggtttgcc agcgaaaatg aacggcaatc gggcaccggc caagagctca tcgctggccg
     4561 attgcagcgc gcagcaacca ccatgaatct ctccgctccg atgacgaccc tgctccaccg
     4621 aatcgccacc cgtatctatg gctcggctgt ggcctggtcc ttcgttttca ctgccatccg
     4681 tgccggtgga aacctgctcg tgctgcctct gatgctgcac aagctcacgc ccgacgatct
     4741 cggtctttgg tatgtctt
//
LOCUS       DATMNS010000259         3397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3089736, whole genome shotgun sequence.
ACCESSION   DATMNS010000259 DATMNS010000000
VERSION     DATMNS010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3089736"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..681)
                     /locus_tag="VNW28_09080"
     CDS             complement(304..681)
                     /locus_tag="VNW28_09080"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXA10123.1"
                     /translation="MILDTNAVSALAMGDAGIIQALAGRERHHLPVIVIGEYDFGLTG
                     LRRSRELQKWLALLVSENIVLGVELATAAYYARLRAELRRAGTPIPANDIWIAALARE
                     HGLPIISRNAHFDHVRGIERLTW"
     gene            complement(678..938)
                     /locus_tag="VNW28_09085"
     CDS             complement(678..938)
                     /locus_tag="VNW28_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10124.1"
                     /translation="MKTTLEIPEPLFRKAKATAAQKGQTLKQFVTEALHDKLGSATRG
                     QKAVGWRVALGQLTPALKKAAREVDAIINAPGFRTIDPNDWK"
     gene            1019..1414
                     /locus_tag="VNW28_09090"
     CDS             1019..1414
                     /locus_tag="VNW28_09090"
                     /inference="COORDINATES: protein motif:HMM:NF020031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXA10125.1"
                     /translation="MHEYQGKAILAANGFKIPRGRSANAADEAVVAAGELKGEVVIKI
                     QAWTTGRAGIGGVAFAKKPEEARAHAERMLAMKVGQVPVEMVLVEEKIAIEREFFLSF
                     AIDDAARAPVVIFSAGGGTGIEERAAESE"
     gene            1453..1929
                     /locus_tag="VNW28_09095"
     CDS             1453..1929
                     /locus_tag="VNW28_09095"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HXA10126.1"
                     /translation="MKTKTLSTSGCIALLCAALLSTATAADPALEEKIRDLDAEWAAA
                     ASAGNVDKTVSYYAANAVVLPANAPVANTKEAIHAQWKKDLEGMVSGSWKANRVEVAE
                     SGDMAWVSGTFDWVSKDASGKKVTDHGKYLEVFQKQADGSWKCTADCWNSDLSATP"
     gene            1984..2397
                     /locus_tag="VNW28_09100"
     CDS             1984..2397
                     /locus_tag="VNW28_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HXA10127.1"
                     /translation="MSDFAELTAFAKRYAEAWCRQHPGNVAAFFAENGSLRVNDAAPA
                     IGRAAIAETARGFMRAFPDMIVTFDKLERQGDRTAFHWTLTGTNTGPGGLGKKVRISG
                     YELWTIDQNGLIAESKGHFDHAEYERQLEHGADEP"
     gene            2360..2863
                     /locus_tag="VNW28_09105"
     CDS             2360..2863
                     /locus_tag="VNW28_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10128.1"
                     /translation="MNASSSTERTSHDSGMNLADLANLGQVVGAIGVIITLVYLALQI
                     RGSTKVASAQARHSLSEFILRISIFRAEHADRFAKLESGVELTAGDRYFQYWSHVQLL
                     LHAETYFHHHELGLMPHTHWRGYARYMSGYVGSAGFREVWDDIGPSFSEDFARWLNGL
                     IASHSSA"
     gene            3060..>3397
                     /locus_tag="VNW28_09110"
     CDS             3060..>3397
                     /locus_tag="VNW28_09110"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HXA10129.1"
                     /translation="MQRRDQISMPRRAAIITALRTPVGRHAGALASVRPDDLAAHIIK
                     AVVARSGIDPALIEDVYFGATNQAGEDNRNVARMAALLAGLPASVPGSTVNRLCASGL
                     EAVNIAARMI"
BASE COUNT          675 a         1048 c         1042 g          632 t
ORIGIN      
        1 cgcgcggccg gcatccttga tcacgtcacg cagcgctttg ccctcctttt tgagattgcg
       61 cctaaagtcg gttcggcccg cgccgagaat gtaaactggt tcgttcatgg ctacctgaaa
      121 cctagactac gcctgctgcg ctgtcatccc caccagtctc gccggatcgg gggcgttgcc
      181 tttgtgaaaa agccggaaga ggcgcggtcc cacgccgagc gcactagcaa cgttacgttt
      241 cttaaagtcg ttaacctgcg agtgggcccg cggttcgaga actaggggtt gccgtcgaaa
      301 ctgctaccaa gtcagtcgct cgattcctcg aacgtgatca aagtgcgcgt tacggctgat
      361 aatcggcagg ccatgctcac gggccagcgc agcgatccag atgtcgttcg ctgggatcgg
      421 tgtgccggcg cgtcttagct cggcgcgcaa gcgcgcgtaa taggccgccg tggcgagttc
      481 caccccgaga acaatgttct ccgaaaccag aagtgccaac cacttctgca actcccggct
      541 gcgccgcaaa ccggttagcc cgaaatcgta ttcaccaatc acgatcaccg gcaaatggtg
      601 acgctcgcga cctgcgagag cttgaatgat tccggcgtcc cccatcgcca gcgcggagac
      661 cgcattggtg tcgaggatca tttccaatcg tttgggtcga tcgtccggaa gcctggcgca
      721 ttgatgatcg catccacctc gcgcgctgct tttttcaggg ccggcgtcag ctgcccgagc
      781 gccacgcgcc agccgaccgc tttctggccg cgcgtcgcgc tgcccagctt gtcgtgcaaa
      841 gcttccgtca cgaattgctt caacgtctga cccttctggg cagcggtcgc tttggccttg
      901 cggaaaagcg gctcgggaat ctccagcgtg gttttcacga gcacgagtca accatattta
      961 tgggtgcctt gcaattcccg cggccggggc tcgaccccac gggccagcca tggcatgact
     1021 gcacgaatat cagggcaaag cgattctcgc cgccaacggg ttcaagattc cgcgcggccg
     1081 gtctgccaac gctgccgatg aagccgttgt cgcggcgggg gaactcaagg gcgaggtcgt
     1141 gatcaagatt caggcttgga ccaccgggcg ggccggcatt ggcggcgtgg ccttcgcgaa
     1201 gaagccggag gaagcacgcg cccacgcgga gcggatgctg gcgatgaaag tcggccaggt
     1261 tccggtcgag atggtgctgg tggaagagaa gatcgcgatc gaacgggagt tctttctttc
     1321 ctttgcgatc gacgacgcgg cccgcgcgcc cgtcgtcatt ttttcggcgg gtggcgggac
     1381 cggaatcgag gagcgcgcgg ccgagtcgga ataagattga cggcccaaca aattcgcggc
     1441 ccgatgggag cgatgaaaac aaaaacgtta tccaccagcg gctgcatcgc cctcctttgc
     1501 gctgccctgc tcagcaccgc aacggcggca gatccagcac tcgaagagaa aattcgcgac
     1561 ctcgacgccg aatgggcggc agcggcctcc gccggaaatg tcgacaagac ggtttcctat
     1621 tacgccgcca acgccgtcgt tctgccggcc aacgcaccgg tcgcaaatac gaaagaggcg
     1681 attcacgcgc agtggaaaaa agatctcgaa gggatggtca gcggaagttg gaaagcgaac
     1741 cgggtggaag tcgcggagtc gggcgacatg gcctgggtga gcggcacctt cgactgggtc
     1801 tcgaaagacg ccagcggcaa gaaggtgacg gatcacggca aatatctcga ggtcttccag
     1861 aagcaggccg acgggagctg gaagtgcacc gccgattgct ggaattccga tctttccgcc
     1921 acgccctaac gtcgccattt cacccgccgg gcggcgggag tcaagcaacc acgcctaacg
     1981 agcatgagtg attttgctga gctcaccgcc ttcgcgaagc gctacgcaga ggcgtggtgc
     2041 aggcagcatc ccgggaacgt ggcggcgttc ttcgccgaga acggatcgct ccgcgtgaat
     2101 gacgcagcgc ctgcgatcgg acgcgcggcg atcgcggaaa ccgcgcgagg attcatgcgc
     2161 gctttcccgg acatgatcgt gacgttcgac aagcttgagc ggcaaggaga tcgaaccgcc
     2221 tttcactgga cgttaaccgg aaccaacacg ggaccgggcg gcctgggaaa aaaggtgcgc
     2281 atcagcggct atgaactttg gacgatcgac cagaacggat tgatcgcgga gtcgaaaggc
     2341 cacttcgatc atgcggaata tgaacgccag ctcgagcacg gagcggacga gccatgacag
     2401 cggcatgaac ctcgcagacc tcgctaatct cggacaggtc gtgggagcga tcggcgtcat
     2461 catcacactc gtttacctcg ctcttcaaat tcggggcagc acgaaggtcg cgagcgctca
     2521 ggcgcgtcat tctctctccg agttcatcct gcgcatttcc atttttcgag ccgagcacgc
     2581 cgatcgattt gccaagcttg aaagcggcgt ggaactcacc gcgggagatc gttacttcca
     2641 atactggagt catgtccaac tcctcctgca cgccgagacc tattttcatc atcacgaact
     2701 gggactcatg ccccacaccc actggcgcgg gtatgcgcgc tacatgtcgg gctacgtggg
     2761 atcggcggga ttccgagaag tctgggacga tattggcccc agcttctcgg aagattttgc
     2821 ccgctggctc aatgggctga tcgccagcca ctcgagcgcc tgacctacgc ggtcgcgtga
     2881 tcggcgacgg cctgcgcgac ttcgagcgtg ctgttgttcc caccctgtcg taggtccgca
     2941 ctttgcctcc cgcgatgact ttcgcgatcg ccagcgccaa aatcgacagc aacgcgggcc
     3001 actccagtat ttcaacagtc tcctagactt gggcagatag ttcccgtagt agattgcgcg
     3061 tgcagcgacg cgatcaaatc tcgatgccaa ggcgagcggc gatcatcacg gctctccgaa
     3121 cacctgttgg tcggcatgcg ggcgcattgg cgtcggtgcg ccccgacgat ctggccgccc
     3181 acatcatcaa ggccgtcgtg gcgcgatccg gcatcgaccc cgcattgatc gaggatgtct
     3241 acttcggcgc caccaaccaa gccggcgagg acaaccgcaa cgtcgcgcgc atggccgcgc
     3301 tgctggcggg cctccccgcg tccgtgccgg gttcgacggt gaatcgcctc tgtgcgtcgg
     3361 ggctggaagc cgtcaacatc gcggcgcgca tgatcga
//
LOCUS       DATMNS010000260         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3100694, whole genome shotgun sequence.
ACCESSION   DATMNS010000260 DATMNS010000000
VERSION     DATMNS010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3100694"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1809
                     /locus_tag="VNW28_09115"
     CDS             <1..1809
                     /locus_tag="VNW28_09115"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HXA10130.1"
                     /translation="NLIAAQAKQRIDLTAEKAYTLSRGTRAILAKLDTPVQIRLYCTR
                     DTKTMPVTLANYAQRVEDLLGEYKQASKGKIEVQQLDPTPDSDAEDSAKLDGVDAQPL
                     RGGERAYLGLSVSMLDQKQAIPLLTPDRERLLEYDISRAIARVMVAKKPVVGVMSALP
                     VMGEMNPMMMMRREQNESRPWAFISELQRDFDVKQVELTTDKIPDEIKVMIVIHPKGI
                     SDATQFALDQFVLRGGQLVAFVDPLCALDRQEASNGGLPPPSSSTLDKLFKAWGVDFH
                     AGQVVADLEFAASVRGERNPTILALNETAMNKDDVLTADADNVLMVFSGVFTGTPAAG
                     LTKTDLIKTTTRSQLIDSMMASFGATQIANDFKASGTQYPLAIRLAGRFKTAFPEGKP
                     KPAQPEPAQPKPAEKPADANFLKESTHPTTVVLAGDADMIQNAVAVRELQTLGGQPLI
                     IPVNGNLGFAQGAVEQLAGDSDLIAVRSRASRERPFTVVQKMQADAEANYRSKIKELE
                     TSLADTQRKMNELQRSKDAGQRFILSPEQQEELTNFRKTEADVKAQLKEMRRKLRAQI
                     DSLENRIKWINIAGMPVAVILVGFLLAALKRQRAGE"
     gene            1806..>2549
                     /locus_tag="VNW28_09120"
     CDS             1806..>2549
                     /locus_tag="VNW28_09120"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HXA10131.1"
                     /translation="MKRNQLALILVLLVALGGAALFVRTRDSASWSNSAAGSSEKILS
                     FSLNDVSRVTIKTSGTELNLVKQEGTWRVAERAEYPADFGKVAGLIRKLWELRATQDV
                     KAGPSQLGRLELLEPGSPSDSGTLIELKDGSAKTMGSLLLGKKQNQMANRPFGEVSLS
                     VGRYVMAPARAEHVYLVAETFDEVQPKPELWLSRDFLKVENPASIALAGISPGLNWKV
                     SRPTAAGPWTLLEAKPGEELDSKKINSLTS"
BASE COUNT          567 a          805 c          752 g          425 t
ORIGIN      
        1 aatctcatcg ccgcgcaggc gaaacagcgg atcgatctca ccgccgagaa agcctacacc
       61 ctttcgcggg gcacccgggc catcctggcc aaactcgata ccccggtgca aatccgttta
      121 tattgcaccc gggatacgaa gacgatgccg gtcacgctcg cgaactacgc tcagcgggtg
      181 gaggatttgc tcggcgaata caagcaggct tccaagggga aaatcgaagt ccagcagctc
      241 gacccgacgc cggattccga cgcggaagat tcggcgaagc tcgatggcgt ggacgcgcag
      301 cccttgcgcg gcggcgagcg agcttatctc ggcctgagcg tcagcatgct cgaccagaaa
      361 caggccatcc ccctgctcac tccggaccgc gagcgcctcc tggaatacga tatttcgcgc
      421 gccatcgcgc gcgtcatggt cgcgaaaaaa ccggtcgtcg gcgtaatgag cgcgctcccg
      481 gtgatgggcg agatgaatcc catgatgatg atgcgccgcg aacaaaacga gtcgcgtccg
      541 tgggccttca tcagcgagct gcaacgcgac ttcgacgtga agcaggtcga gctcacgacc
      601 gataagatcc cggacgagat caaggtgatg atcgtgattc acccgaaagg aatatccgac
      661 gccacgcagt tcgcgctcga ccaattcgtc ctgcgcggcg gccaactggt cgcgtttgtc
      721 gatccgctct gcgcgctcga tcgacaagag gcttcaaacg gcggattgcc gccgccgagc
      781 agttcgacgc tcgacaaatt gttcaaggcc tggggcgtgg acttccacgc ggggcaggtc
      841 gtcgcggatc tcgaattcgc cgccagcgta cgcggagagc gtaacccgac cattctcgcg
      901 ctgaacgaaa ccgcaatgaa caaggacgac gtgctgaccg ccgacgcgga caacgtgctc
      961 atggtcttca gcggcgtgtt caccggcacg ccggcggccg gtttgaccaa gacggacctc
     1021 atcaagacca cgacgcgctc gcaactgatc gactcgatga tggcttcctt tggcgcgacg
     1081 cagatcgcta acgatttcaa agccagcggg acgcagtacc cgctcgcgat ccgtttggcc
     1141 ggtcgtttca aaaccgcatt cccggagggc aaaccgaagc cggcccagcc ggaaccagct
     1201 cagccgaagc cggcagagaa acctgccgac gcaaactttc tgaaggagtc cactcatcca
     1261 accaccgttg tcctggcggg tgacgccgac atgatccaaa acgcggtcgc agtgcgcgaa
     1321 ctgcagacgc tcggcggaca accgctcatc attccggtga atggcaacct cggcttcgcg
     1381 cagggcgccg tcgagcaact ggccggcgac agcgacctca tcgcggtgcg cagccgcgct
     1441 tcacgggagc gcccgttcac tgtcgtccag aaaatgcagg cggatgcgga agcaaattac
     1501 cgcagcaaaa tcaaggaact cgaaaccagc ctggccgaca cccagcgcaa gatgaacgag
     1561 ctgcaacgga gcaaggacgc cgggcaacgc ttcattcttt cccccgaaca acaggaggag
     1621 ttgacaaatt tccgcaagac cgaagctgat gtgaaagcgc agttgaaaga gatgcggcgc
     1681 aaactgcggg ctcaaatcga ttcgctcgag aatcggatca agtggatcaa tatcgcgggc
     1741 atgccggtcg cggtgatcct cgttggattc cttcttgccg ccctcaagcg gcagcgcgcc
     1801 ggagaatgaa aaggaatcag ctcgccctca tcctcgtcct gctcgtcgcc ctcggtggcg
     1861 ctgccctttt tgtccgcacc cgcgactccg cgtcctggag caacagcgcg gccgggtcga
     1921 gtgaaaagat tctttcgttc tcgctcaatg acgtttcgcg ggtgaccatc aaaaccagtg
     1981 gaaccgaact caacctggtc aagcaagagg gcacctggcg ggtggcggag cgcgctgaat
     2041 accccgccga tttcggaaag gtggccggct tgatccgcaa gctctgggaa ttgcgcgcca
     2101 cgcaggatgt caaagccggc ccttcgcagc tagggcgtct ggagctactc gagcccggca
     2161 gcccttcgga cagtggcacg ttgatcgagc tcaaggacgg cagcgcgaaa acaatgggct
     2221 cgttgttgct cgggaaaaag caaaaccaaa tggcaaaccg gcctttcgga gaagtgtcgc
     2281 tctcggtcgg gcgctatgtc atggcgcccg ctcgcgccga acacgtctac cttgtggcgg
     2341 agaccttcga tgaggtccag cccaaacccg aactctggct cagccgcgat tttctcaagg
     2401 tggaaaatcc cgcatccatc gccctcgccg gaatttctcc gggcctgaat tggaaagtct
     2461 cgcgcccgac cgccgccgga ccctggacgt tacttgaggc gaaacccggc gaagaactcg
     2521 acagcaagaa gatcaacagc ctcaccagc
//
LOCUS       DATMNS010000261         3649 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3101665, whole genome shotgun sequence.
ACCESSION   DATMNS010000261 DATMNS010000000
VERSION     DATMNS010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3101665"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1116)
                     /locus_tag="VNW28_09125"
     CDS             complement(22..1116)
                     /locus_tag="VNW28_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10132.1"
                     /translation="MHSSTSNFERVIPTRPWRGITVSVVMILAVATAAWEIYCRSLGY
                     GPSLNDTGDLWAEARRRVEPESLVIVGDSRALFDSDLDQLEEGLGKRPIQLAQPGSTA
                     FPVLADLINDQSFHGTILCSVVPRLFFAPSGSPPMNRSEKSVRRAHTQTYAQRASHDL
                     GIYLEEWCACMKQDDLTLGALLGRLPIPNRSNAQVPPPLPPYFGSLDRERRARMVEQC
                     ARPGELQERVKHRWARLFTPPPPPSFVPQEVFAKKMQAAFEARFADTKALVDKLRARG
                     GRIVFVRYPVSGDLKAFEDQTTPRGPTWDRLLKETAAPGIYFEDFPELAGFTCPEWSH
                     LSAGDSVEFSRRLVPHLRTALDAGSVASAR"
     gene            complement(1091..2512)
                     /locus_tag="VNW28_09130"
     CDS             complement(1091..2512)
                     /locus_tag="VNW28_09130"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HXA10133.1"
                     /translation="MLFNSLTFVVFFVVVVSAYWSMRSWPARKNLLVVASYVFYGAWN
                     PPFAALLFGTTAMDFYLGRIMGKQERPEARKVWLVISVAVNLSMLGFFKYGNFLLENF
                     QWVLSRGGIAYHPPHLDLFLPIGISFYTFHSLSYTLDIYRGVLKPTRSLRDFVLAVSF
                     FPQLVAGPIVRAGDFLPQLAAPPKPQQGRFFWGLLLMTLGLFEKVVLADTLLAGSADT
                     IFGYGGPLVALDSWMGVMAFAGQIFFDFAGYSICAIGAALCLGFHLKDNFRFPYAAIG
                     FSDFWRRWHISLSTFLRDYLYIPIGGNRAGAARAMVNLMIVMFIGGLWHGAAWTFVVW
                     GLLHGSYLALERLTKVIVGEPGWANHLSVRLFLGLGTYFAVCVAWVFFRASDFTVAAR
                     MLSGMFGGHPHGDALLSTRELLQIGVVTVFMIAVHWSLRDTTIEAAVNRMPVWVLTGL
                     WTLMAAAILLTQGNSNAFIYFQF"
     gene            complement(2635..3390)
                     /locus_tag="VNW28_09135"
     CDS             complement(2635..3390)
                     /locus_tag="VNW28_09135"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXA10134.1"
                     /translation="MFKAFRTWCEEVLRIPGDPEPPPGDEARTQRFRAAPNYYRYLLG
                     LWAVKTAAVFIVLVTMEMGAIVELFKAPQQKLPGALVVGLPQIIVLFFFIGRLLSLAL
                     VRLDFEKRWYLVTDRSLRLREGIVHVREMTITFANIQNISISQGPLQRLLGIADLRVD
                     TAGGSAPRRRERKGMPQSLHTAFFRGVDNADAIRELIQTRLRELKDSGLGDHEEAARA
                     TPPAALRVPSAQLLAALREVAAEATALREAVGR"
     gene            complement(3383..>3649)
                     /locus_tag="VNW28_09140"
     CDS             complement(3383..>3649)
                     /locus_tag="VNW28_09140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10135.1"
                     /translation="IQIQTASGSADAELVIEGFKEYEAIRDFLYTRMRGYHAPAKAAP
                     VQTAAALEESADPNAEVISLLRTIRDELRQTRESLGKPPATGHV"
BASE COUNT          805 a         1083 c         1174 g          587 t
ORIGIN      
        1 tcatgcagca gacttcggtc gttaacgagc ggacgcaacc gatcccgcgt cgagcgcggt
       61 gcggaggtgc gggacgagcc ttcggctaaa ttcgacggaa tctccggcgg aaagatgcga
      121 ccattcggga caagtgaatc cggccagctc cgggaaatcc tcgaagtaaa tgcccggcgc
      181 ggcggtctcc ttgaggaggc gatcccaggt cggtcctcgc ggggtcgtct ggtcttcgaa
      241 ggctttgaga tcgccgctca ccggatagcg cacgaagacg attcgcccgc cacgggcacg
      301 caatttatcg acgagtgctt tcgtgtcggc aaagcgggct tcgaaggcgg cctgcatttt
      361 tttcgcgaag acttcctgcg ggacaaagct cggaggtggc ggcggagtga agagccgagc
      421 ccagcgatgc ttcacccgtt cctgcaattc tcccggccgc gcgcattgtt ccaccatgcg
      481 ggcgcggcgc tcgcgatcga gcgagccaaa gtagggcgga agcggcggtg gcacctgagc
      541 gttggagcga ttcggaatcg gcaaccgacc gagcaacgcg ccgagggtaa ggtcatcctg
      601 cttcatgcag gcgcaccatt cttcgagata gatgccgaga tcgtgactcg ctcgctgggc
      661 atacgtctgg gtatgtgcgc ggcgcaccga tttctcgctc cggttcatcg gaggcgaccc
      721 cgatggggca aagaagagcc gcgggacgac gctgcaaaga atggtgccgt gaaaactttg
      781 atcattaatc aagtcggcca ggacgggaaa cgcggtgctc ccgggctgag cgagttgaat
      841 ggggcgcttg cccaatcctt cttcgagttg gtcgaggtcg gagtcgaaga gcgcgcgcga
      901 gtcgcccacg atgacaagcg actccggctc gacgcggcgg cgtgcttcgg cccagagatc
      961 gccggtatcg ttcaggctgg gcccgtagcc gagcgaacgg caatagattt cccaggccgc
     1021 ggtcgccacg gccaggatca tcacgacact gaccgtgatg ccgcgccacg gccgggtagg
     1081 gattacccgc tcaaaattgg aagtagatga atgcattgct gtttccttgg gtgagcagga
     1141 tggccgcggc catcaacgtc cagaggccgg tcaggaccca gaccggcatg cggttgacgg
     1201 cggcttcgat cgtcgtatcg cggagcgacc aatgaaccgc gatcatgaaa acggtcacaa
     1261 ccccgatctg caaaagctcg cgcgtgctga ggagcgcgtc gccgtgcgga tgtccgccga
     1321 acatgccgct cagcatccgt gccgcgaccg taaaatcgga ggcgcgaaag aacacccacg
     1381 cgacgcaaac cgcgaagtag gtgccgagac cgagaaacaa tcttaccgac agatgattcg
     1441 cccaacccgg ctcgccgacg atgactttcg tcaggcgctc gagcgcgaga taggaaccgt
     1501 ggagcaaacc ccagacgaca aaggtccagg ccgcgccatg ccagagaccg ccgatgaaca
     1561 tcacgatcat gagattgacc atggcgcgcg cggccccggc acggttgccc ccgatcggga
     1621 tgtaaaggta atcgcgcagg aaagtagaaa gcgagatatg ccagcggcgc cagaaatccg
     1681 aaaaaccgat cgcggcatag ggaaaacgga agttgtcctt taggtgaaac ccgagacaca
     1741 aggccgcgcc gatcgcgcag atcgagtagc cggcgaaatc gaaaaagatc tggcccgcga
     1801 aggccatcac gcccatccac gagtcgagcg caacgagcgg cccgccataa ccaaagatcg
     1861 tatcggccga gccggcgagg agcgtatcgg cgagcacgac tttttcgaaa aggcctaacg
     1921 tcatcaagag caggccccag aagaaacggc cctgctgtgg tttcggcggc gccgcgagtt
     1981 gcgggagaaa atcacccgcg cgcacgattg ggccggccac gagttgcgga aagaaggaaa
     2041 ccgcgagcac gaaatcgcgc agggaacgcg tcggcttcaa caccccgcga taaatgtcga
     2101 gcgtgtagga gagcgaatgg aaggtgtaga acgaaatccc gatcgggaga aagagatcga
     2161 gatgcggcgg gtggtaggcg atcccgccgc gcgacagaac ccactggaaa ttctcgagga
     2221 gaaaattccc gtatttgaaa aaaccgagca tgctcaggtt gaccgcgaca ctgatcacca
     2281 gccagacctt gcgcgcttcc gggcgttcct gtttccccat gatccggccg agataaaaat
     2341 ccattgccgt cgtgccgaag aggagtgcgg cgaaaggcgg attccacgcg ccataaaaaa
     2401 cgtagctcgc gaccacgagc agatttttgc gcgccggcca cgagcgcatc gaccaatacg
     2461 cgctcacgac cacgacaaaa aaaacgacga acgtcagcga gttgaagagc acgcgcccgc
     2521 tttatagagg cacggacgag gacgacaagc agaattcacc ggtctagttt gccaggaagc
     2581 gacgaatcgg agatcaaggc ggagcgatgc cgcgccgaga ggccctccca ttgcctaacg
     2641 gccaaccgct tcccgcagcg cagtagcttc cgccgcgacc tcgcgcaagg cggcgagaag
     2701 ttgcgcggaa ggtacgcgca acgctgcagg tggcgtcgcg cgcgccgctt cttcgtggtc
     2761 gcccagaccg gaatccttca gctcgcgcag gcgggtctgg atcaactcgc ggatcgcgtc
     2821 cgcgttgtcg accccgcgaa aaaacgcggt gtgcaggctt tgtggcatcc ctttgcgttc
     2881 gcggcgcctg ggggccgaac cgccggcggt gtcgacccgc aaatcggcga tgccgaggag
     2941 gcgctggagc ggaccctgcg agatcgagat gttctggatg ttggcgaagg tgatcgtcat
     3001 ttcgcgcacg tgcacgatgc cttcgcgcag gcgcaggctg cgatcggtca cgaggtacca
     3061 gcgtttctcg aaatcgagcc taacgagcgc gagggaaagg agccggccga tgaaaaaaaa
     3121 gaggacgatg atttgcggaa gaccgacgac gagggctccg ggcagctttt gttggggcgc
     3181 cttgaagagc tcgacgatcg cccccatttc catcgtcacc aggacgatga acacggccgc
     3241 cgtcttcacg gcccagagtc cgagaagata ccggtagtaa ttgggtgcag cccgaaagcg
     3301 ctgggtgcgc gcttcatcgc ccgggggcgg ttccggatcg cccggaatgc gcaggacctc
     3361 ttcgcaccag gtgcggaagg ccttaaacat gacctgtcgc cggcggcttg cctaacgact
     3421 cccgggtttg gcgcagctcg tcgcgaatcg tccgcaggag cgaaatcact tccgcgttcg
     3481 gatccgcgct ctcctcgagc gcggctgccg tctgcacggg cgctgctttc gccggcgcgt
     3541 gataaccacg catccgggtg tagagaaaat cgcggatcgc ttcgtactcc ttgaatcctt
     3601 cgatcacaag ctccgcatcg gccgagccgg acgcggtctg gatttggat
//
LOCUS       DATMNS010000262         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2035064, whole genome shotgun sequence.
ACCESSION   DATMNS010000262 DATMNS010000000
VERSION     DATMNS010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2035064"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VNW28_09145"
     CDS             <1..387
                     /locus_tag="VNW28_09145"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXA10136.1"
                     /translation="FKNHHVTLSRGGHDAPLLYRGATGEVEVLKPPGMALGIDSGDVF
                     DRITGDISVPLERDDCLVLYTDGVTEALDANGDEFGPERMIRSIRASASEGAQGVITR
                     LTSDVRNFAGSHPQHDDITLIVVRKT"
     gene            384..956
                     /gene="grpE"
                     /locus_tag="VNW28_09150"
     CDS             384..956
                     /gene="grpE"
                     /locus_tag="VNW28_09150"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXA10137.1"
                     /translation="MKNSKTDRRRIPVTGEAMPNDTAATTAATGPDAPDNNDDPTAGL
                     QADLDRFRDLALRSQADFDNFKKRATREKEDAIKYANSSLLERLIAIVDNFELGLSAA
                     KSEGEESPIYSGMNMVLKQLTDFLAENGLQPIAAEGEMFDPNLHEAIAHEPSDGVPEG
                     VVLRQMRRGYRLKDRLLRPSSVVVSSGSAA"
     gene            1057..2190
                     /gene="dnaJ"
                     /locus_tag="VNW28_09155"
     CDS             1057..2190
                     /gene="dnaJ"
                     /locus_tag="VNW28_09155"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXA10138.1"
                     /translation="MTTKRDYYEVLSVERSASGEEIKRSYRKLAVKFHPDKNPDDPHA
                     EEKFKELGEAYDVLMDEQKRAAYDRYGHAAFTQGGRAGGFHDPFDIFREVFSGAGGTG
                     GIFETFFGGGGQAGHDGTQRGSDLRYDMQITLEEAAFGVEKEIEVRKLDTCDKCRGSG
                     AAPGSRAVTCTTCRGRGQVVSSRGFFQVSQTCPRCRGLGEVIEKPCEVCDGEGRVEKT
                     SRIKIKIPAGIAHGSRLRSTHNGEAGVRGGDQGDLYIVVHIKEHPIFQREDDNLFCEV
                     PISFATAALGAEVPVPTLEGKASLKVPAGTQSGQSFKLRGKGVVQVNGRERGDLYARL
                     LVEVPTDLNAEQRQKLEEFAALCGENNTPLHKSFFERAREFFR"
     gene            2240..3094
                     /locus_tag="VNW28_09160"
     CDS             2240..3094
                     /locus_tag="VNW28_09160"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HXA10139.1"
                     /translation="MHRFHLAPNDWPSATLTGSEAHHARSVLRLQPGDRVVLFDGCGR
                     EATAEIAALEATAVLVRILHEAVSPPLRARITLAQAIPKGKNMDLIVQKAVEIGAAEI
                     FPLLSARSVVQLDPESAAQKQAKWQTTALEAAKQCGQNWLPHVHTPRTPNDFFREIAS
                     GVSLAGGEAGVSPANPQRVASSDQPPPRLARPSLPFDLRLVGSLQPGALHLKRILADY
                     AQAHEGARPANVLMLIGPEGDFTPAEIGLARSHGCAPLTLGPIILRVETAAIYCLSIL
                     SYELLSER"
     gene            complement(3163..3645)
                     /locus_tag="VNW28_09165"
     CDS             complement(3163..3645)
                     /locus_tag="VNW28_09165"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXA10140.1"
                     /translation="MQLFTRLGLAAIVVSGLVTAAQAQISQFSISYDGEQWRNSGSTV
                     PSVLTLQLSLDGTTWVSLGAAVNFTSPINTATAGLLDGNALANRVAGIGGTFTPSTPI
                     PNGSVFYLRWADPDDTGADAGLAVDNFTFSAVPEPSIYVLLGVGILLCGQRFLRGRRS
                     "
BASE COUNT          783 a         1260 c         1179 g          690 t
ORIGIN      
        1 ttcaaaaacc accacgtcac gctctctcgt ggcggccacg atgccccctt gctctaccgc
       61 ggcgcgacgg gcgaagtgga ggtcctgaag ccgcccggga tggcccttgg aattgacagc
      121 ggagacgtgt tcgataggat aacgggcgac atttccgtcc ccctcgaacg cgatgattgc
      181 ctcgtgcttt acaccgacgg cgtcaccgag gcgctcgatg cgaacggaga tgaatttggc
      241 ccggagcgaa tgatccgatc catccgcgcc agcgcgagtg aaggggcgca gggggtgatc
      301 acgcgcctga ccagcgatgt ccgcaatttc gcgggtagcc atccgcagca tgatgacatc
      361 actttgattg tagttcgaaa gacatgaaaa attccaaaac cgaccgccgc cgcattcccg
      421 tcaccgggga ggcgatgcct aacgacaccg cggccacgac tgccgcgacc gggccggacg
      481 ctccggacaa taacgacgac ccgaccgccg gtttgcaggc cgatctcgat cgctttcgcg
      541 acctcgcgct gcgcagccag gccgacttcg acaacttcaa gaagcgtgcg acccgcgaga
      601 aggaggacgc catcaagtac gccaatagct cgctcctcga gcgcctgatc gcgatcgtcg
      661 ataatttcga gctcggcctc tccgccgcga aaagcgaagg cgaagaatcc cccatctatt
      721 ccggcatgaa catggtcttg aagcagttga ccgacttcct cgccgagaac ggcctccagc
      781 ctatcgcggc cgagggtgaa atgttcgatc ccaacctgca cgaagcgatc gcgcacgaac
      841 cgagtgacgg cgtgcccgaa ggcgtggtct tgcggcagat gcggcgcggt taccgcctca
      901 aggatcggct cctccggccc tccagcgtcg tcgtgtcgag cggatcggca gcctagaagt
      961 tcttctctcg tgcccgcgct cgtgatcgtc atcgtgctcg aaaacatttt acagatcgat
     1021 tacgagcacg cgtaagagca tgcgtaagat ttcctcatga ccacgaagcg cgattactac
     1081 gaagtcttga gcgtcgagcg cagcgcctcc ggcgaggaga tcaagcgttc ctaccgcaag
     1141 ctcgcggtta aattccaccc cgacaaaaat ccggacgacc cgcacgcgga agagaaattc
     1201 aaggagcttg gtgaagccta cgacgtcctg atggacgagc aaaaacgcgc cgcctacgat
     1261 cgctacggac acgccgcttt tacccagggt ggtcgcgccg gcgggttcca cgatccgttc
     1321 gatatcttcc gcgaagtctt cagcggcgcg ggcggcacgg gcggcatttt cgagaccttt
     1381 ttcggcggcg gcggccaagc cgggcacgat ggcacgcagc gcggaagcga tctgcgctac
     1441 gacatgcaga tcacgcttga agaagcggcc ttcggcgtcg aaaaagaaat cgaagtgcgc
     1501 aagctcgaca cctgcgacaa gtgccggggg agcggggcgg cgcccggctc gcgcgccgtc
     1561 acctgcacaa cctgtcgtgg gcgcggccag gtggtcagct cgcgcggatt tttccaggtc
     1621 tcgcagacct gcccgcgctg ccgcggcctc ggcgaagtga ttgaaaaacc gtgtgaagtt
     1681 tgcgacggcg agggtcgggt cgaaaaaacc agccggatca aaatcaaaat ccccgccggc
     1741 atcgcccacg gttcgcgcct gcgctcgacc cacaacggcg aagcgggtgt gcgcggcggc
     1801 gaccagggcg acctctacat cgtcgtccac atcaaggaac acccgatttt tcagcgcgag
     1861 gacgacaatc ttttctgcga ggtgccgatc tccttcgcga cggcggcgct gggcgcggag
     1921 gtgccggtgc cgacgctgga agggaaagcg agcctgaaag ttcccgccgg cacccagagc
     1981 ggacagagtt tcaagctgcg gggcaaaggc gtggtccagg tgaacggccg cgagcgcggc
     2041 gacctctacg cccgcctgct ggtggaagtg ccgaccgatt tgaacgccga gcagcgccag
     2101 aagctggaag aattcgccgc gctctgcggc gagaataaca ccccgctgca caaaagcttc
     2161 ttcgagcgcg ccagggaatt cttccggtaa ggaaaaggat gaactaagag ggacgagttc
     2221 cgcttcttct tcttttttca tgcaccgctt tcaccttgcc cctaacgact ggccttcggc
     2281 caccctcacc ggctccgagg cgcaccacgc gcgcagcgtc ctgcgcctcc aaccgggcga
     2341 tcgggtcgtc ctcttcgatg gctgcggccg cgaagcgacc gccgagatcg ccgcgctcga
     2401 ggccaccgcc gtactggtgc gcatcctgca cgaagcggtc tccccgccgc tgcgcgcccg
     2461 gatcacgctc gcccaggcca ttccgaaggg aaagaacatg gatctcatcg tgcagaaagc
     2521 ggtcgagatc ggcgccgccg agatttttcc tctcctttcc gcccgcagcg tggtgcagct
     2581 tgacccggag agcgccgcgc agaagcaggc caaatggcag accaccgcgc tcgaggccgc
     2641 gaaacaatgc ggccagaact ggctcccgca cgtgcacact ccgcgcacgc ctaacgattt
     2701 cttccgcgag atcgcatccg gcgtctcgct cgccggtgga gaggcgggcg tgtcgcccgc
     2761 caatccgcag cgggtcgcat cgtcggacca gccacctccg cgcctggcac ggccgtcgct
     2821 accctttgac ctgcggctag tcggttcgct ccagccgggc gcgttgcact tgaaaaggat
     2881 cctggccgat tacgcacagg cgcacgaagg cgcgcgcccg gctaatgttc tcatgctcat
     2941 cggaccggaa ggcgatttca cgcccgcgga gatcgggctc gcgcgctcgc acggctgcgc
     3001 gccgctcaca ctcggcccca tcatcctgcg ggtggagacc gccgcgattt actgcctgag
     3061 cattctttcc tacgaactgc tatcggagcg ttaggcagtc gttgcgcaac aaaaaacccc
     3121 gcaccggctg acgcggggtt tttcgcagga gatagagatc cgttaggacc gcctgccgcg
     3181 caggaagcgc tgaccgcaaa gcagaatgcc cacaccgagg agcacataga tggacggctc
     3241 gggaaccgcc gagaaggtga agttgtcgac cgcaaggccg gcatcggccc cggtgtcatc
     3301 cgggtcggcc cagcgcaggt aaaacacgga tccgttcgga attggcgtgg acggcgtaaa
     3361 ggttccgcca attcccgcta cccgattcgc caaagcgttt ccgtcgagaa gacccgccgt
     3421 agcggtattg attgggctgg tgaaattgac ggccgcccca agcgaaaccc acgtcgtccc
     3481 gtcaagactc agttgcagcg tcaagacact aggaacggtg gaaccactgt tgcgccactg
     3541 ttcaccatcg taagaaatac tgaactggct gatttgagct tgcgcggcgg tcacgagtcc
     3601 cgagacgacg atggcagcta atccgagcct cgtaaataat tgcattctga ctttttccgg
     3661 tcttttggct tatttgtctc tctgagcgcg ccgatcatgt cagcccgccg tccggttggc
     3721 aatacttttc ttccattgat ttgaagttcc tatcgtcttt gagcagccgt tgcggcgccc
     3781 ccgggcaggc ggctagtgtc gcggaagaac tggcttgggc gcttaaccac aaaaaaagcg
     3841 cctcggtttc ccgaagcgct ttctgaaaat gttattctcg gttgccgctt agcgtccccg
     3901 ccgctggccg cc
//
LOCUS       DATMNS010000263         9063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3465675, whole genome shotgun sequence.
ACCESSION   DATMNS010000263 DATMNS010000000
VERSION     DATMNS010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9063
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3465675"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            636..1595
                     /locus_tag="VNW28_09170"
     CDS             636..1595
                     /locus_tag="VNW28_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXA10141.1"
                     /translation="MRVFSFVGPPQSPKSGRILVGTASWSDPGFVERWYPPKLPAGDR
                     LPWYAEHFEMVEVNSTFYAVPEVRLVERWVRATPNEFVFDVKLHRLLSRHAATLRSLP
                     PALQRVAEADAGGKVKLTADLERALLEQIVAAIEPLRAAQKFGAFLLQLSPAFSPRKH
                     DLAELDGLLARLAPLGAVVELRNRHWTEDGHLTKTLEFFRRHHTTFALVDAPAEAHFT
                     IMPSDLDAVTRRALAYLRLHGRDAQAYLRGRTVAERFSYDYSDEEIGAVAERAKKLAR
                     EAEAVHVVFNNNALDFAPHAALRLRAALGQITKGPPREGDLFR"
     gene            1608..2108
                     /locus_tag="VNW28_09175"
     CDS             1608..2108
                     /locus_tag="VNW28_09175"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HXA10142.1"
                     /translation="MDQPPSSQPGVWARLRRSASGLHFRLVTIEDTPHSIALGVAIGI
                     FFGFTPLWSLKTLLSIAVAWVCKSNKIAAAISVQLHDLILPFMPAIYLWEYKFGYWAM
                     RGHLPRRVNFRTLEFRDYVHWEMFFSVGRPLLIGSLIIGLPSAALVYVICRALVTSHR
                     ATRAEP"
     gene            2212..2889
                     /locus_tag="VNW28_09180"
     CDS             2212..2889
                     /locus_tag="VNW28_09180"
                     /inference="COORDINATES: protein motif:HMM:NF025724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4410 domain-containing protein"
                     /protein_id="HXA10143.1"
                     /translation="MKPLIAIASSLLLCSCADMVVTKSAVSSSSGRATVEAKDFDPKG
                     GVHYETCGVGAANPAAIYIRPFCIDSAIFRGDEAPSDGEMPIRKALAPVAFANDLREE
                     LGKMAPSLVIAENEVPTIGWLVEGQFDLVDGGDPLARFFFGPFGAGQSMLALHVKVTE
                     VATHHVIYEFDMAGGSHEQGPLGTVRASGLGKATPFDLRNAAERVLVALSSDPYRYGA
                     RASLSLR"
     gene            2976..3944
                     /locus_tag="VNW28_09185"
     CDS             2976..3944
                     /locus_tag="VNW28_09185"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXA10144.1"
                     /translation="MIVFSRKILLLAAVLALFSGAPRALHGAEATITVDAKTGLILDK
                     LQSDKKRQVGSLTKIATAMVVLDWAAKESGDLGQVAVIPEAAFAGTSENNVGFQPGDT
                     VTLRDLLYAALVQSDNIAAYTLADHVGQALLRRVPPSGEAARGTPVDYFVLQMNALAQ
                     HLGMMRTRFLNPSGLDAKEKPFSTAADIARLTRYAMNNAGFRFYVSQKERGIEIGRGA
                     GRLRYLLRNTNELLGRDEIDGVKTGRTLRAGDCVVLSAARPSEVVQQGATTLVTPRRL
                     IVVVLGSPDRFAEGAALLARGWSLYDRWAAAGRPVSPKTTLGGPNE"
     gene            3944..5020
                     /locus_tag="VNW28_09190"
     CDS             3944..5020
                     /locus_tag="VNW28_09190"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HXA10145.1"
                     /translation="MKPRIGVLTSGGDCPGLNAVLRGVVLAAEKLRWEVVGFKDGFEG
                     LLPPGHYMILDRARTAGITPLGGTIIGTTNKGHFVAKVGAGNRAIVAREVIEQARSVL
                     GHLHVTALIIVGGDGSMTTALQLQGEGINCIGVPKTIDNDLEATAMTFGFDSAVACVA
                     DALDRLHTTATSHKRIMVLEVMGRHAGWIALHGGIAGGADAILIPEIPFDYEKLADFI
                     NRREAAGSRSSMVVVAEGATPKDGQQVLHQTEAGEYRFGGIGEVVAHELAARTGKDTR
                     CCVLGHLQRGGAPTTLDRILGTRFGVKAVQLINEGHFGSMVSYQNYQVQHVPIADAVN
                     RLRLVQTSSQMVETARDVEISFGD"
     gene            complement(5119..5784)
                     /locus_tag="VNW28_09195"
     CDS             complement(5119..5784)
                     /locus_tag="VNW28_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HXA10146.1"
                     /translation="MSASGPPLFGAIFDWDGVIIDSAGLHEQSWRRLAQELGKTIAAD
                     SFIRGFGMKSARIIEEIHGWAKEPAEIARLTNRKEALYREIVARSEIAPLPGVVAWLE
                     RLRAADVPCAVASSTQRLNIDTVLERIGLKVAFREIVSAEDVVHGKPNPEVFQKAATR
                     LGVAAERCVVFEDAHVGIEAAHAARMKVVAVPTTHPVEQLAAADLVVRRLDELTVEQV
                     ARL"
     gene            complement(5781..7301)
                     /locus_tag="VNW28_09200"
     CDS             complement(5781..7301)
                     /locus_tag="VNW28_09200"
                     /inference="COORDINATES: protein motif:HMM:NF036494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXA10147.1"
                     /translation="MRTTALFLIALLAAFAPAEAKKAPVPVKTLPPMAAQAAVQAKEV
                     SLVRVNVTGQPFDYFRPWQKKAPFSKRALGAVLAHDRVLVTADLVENQNYVELERAES
                     GEKTAANVVVVDYEANLALLEPSDKKFLNGLQPLDLALDTVVGDRLAAWQLEATGTLV
                     VTEGIVTTIGVTRYPADVSDFLTYRLSIPMQYRDNSYTVPLVKNNKLAALLLRFDPRS
                     QTLDAIPAPIIAHFLKDASGPKYEGFPSLGISFFPTRDPELRAYAGATGKPGGIYVTS
                     VEPGQPADKAGLQRGDIVTAVGDHAVDENGNYVDALYGKLEVSNLLTGKAYVGESVAL
                     HVQRAGKPLTINVKLEHRAEEDYVVPPYNIDDPPPYYVLGGLIFQELSRQYLKEWGAN
                     WQKDAPQDFVYLDKFQSELFPLGKRRVVILSQVLPANSTIGYDDFSYLVVKKVNGKVI
                     NSLGDLAEAVKTPVNGFHVIETADDPKQLELEASEVAAEADALQKNYGLPALQRLP"
     gene            complement(7304..8803)
                     /locus_tag="VNW28_09205"
     CDS             complement(7304..8803)
                     /locus_tag="VNW28_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXA10148.1"
                     /translation="MHRFRFLICALALFLVVEAHGQPNGQIQNSLVRITSTEVSPDYR
                     APWNSGSIGRGVGAGFVIDGQRILTNAHVVSNSRYLTVEREGDPNKYPATVQFVAHDC
                     DLATVQVAAPNFWKGMKPLSFGGIPALESTVSAYGYPIGGERMSVTTGIVSRIDFNLY
                     THSAIDSHLAVQISAQINPGNSGGPVMQTGKVVGVAFQGYSGDIAQGVAYMIPTPVIR
                     RFLKDIEDGHYDRYVDLGITYLKLQNPAQRHYLGLKDDDRGVLVGTVIDAGPCGKLLK
                     SGDVLLSIDGYPIASDAFVEYEGERVQMPEVVERKFKGDTVKLDLWRNQQPMSVVITL
                     GSVWPYFMQGHSYDIRPRYVLYGGLLFQPMSLDLLEAYRIGDLRVRHFFDFFVTEQIY
                     LQHPEVIILTNILPDPINTYLLPFRSSIVDEINGQKIGTLQDLARAFEQPADRFVVKM
                     VGDGPPLVLDPKQVEAARERIKTRYNVFQEQQLDEQPPAKPAVAAQTNS"
BASE COUNT         1658 a         2741 c         2897 g         1767 t
ORIGIN      
        1 ccccggcaac cagttttttg gcgcggacag ttggccggcc ccgccaacct gcccccaacc
       61 aaaccctctc gtggcggatt accgcgtcta cattttgctc aacgaggtgg accgacgcta
      121 catcgggctg agcgaaaacc cactgctccg actccagcaa cacaacgctg gtctctcgaa
      181 gtggaccagg aatagaggtc cgtggcggat catttggcaa agtcaggaat tgtcgcttac
      241 cgatgcgaga aaaattggaa aacaaactga agaggcagaa gggcggccga ggtgtcgatc
      301 acgtaatcgg atcgtcaggc ccataatccc gcctcggagg cgggaccgcg cgttcaaatc
      361 cgcccccgca accagttttt taggactgcg cctcggcgcc cacccgcgga gccgacgaaa
      421 tctttacgac ttcaatcttg gcgggtaatc cggcgaggga ttgcgcgcaa agatggccag
      481 cgaagcccgc ggcgagccag ccgatggtgg ccaggcaatc caatcgagtg aagcgatcaa
      541 acctcacgaa acggcattaa atggccgcgg gcgatccgga gcaagcttgt ttggcgcccg
      601 ccgcggtcgt tcggcgtggt tgcgtttgcc cgccggtgcg cgttttctct tttgtggggc
      661 cgccgcaatc tccgaaatct ggccgcatcc ttgtcggcac cgcgagctgg tcggatcccg
      721 gttttgttga gcgctggtat ccgccgaagc ttccggcggg cgatcggttg ccgtggtacg
      781 cagagcattt cgagatggtc gaagtgaact cgaccttcta cgccgtgcct gaggttcgtc
      841 tcgttgagcg ttgggtgcgc gccacgccta acgaatttgt tttcgacgtc aagttgcacc
      901 gcttgctttc ccgtcacgcc gccacactga ggtcgctccc gcccgcgctc cagcgcgtcg
      961 cggaagctga cgcgggaggg aaggtgaagc tgacggcgga cttggagcgc gcgttgcttg
     1021 agcagatcgt cgcggcgatc gagccgttgc gcgccgcgca gaaattcggc gcttttttgc
     1081 tgcaactttc gcctgccttt tcgccgcgga aacatgatct cgcggaactg gacggcctcc
     1141 tggcgcggct cgctccgtta ggcgcggtgg tggagttgcg gaatcgccat tggacggagg
     1201 atggccacct gacgaagacg ctggagttct tccggcgcca tcacacgacc ttcgcgctgg
     1261 tcgatgcgcc ggctgaggcg catttcacca ttatgccgag cgacctcgat gcggtgacgc
     1321 gtcgcgcgct ggcctatttg cgcctccacg gccgcgacgc gcaggcctac ctgcgcggcc
     1381 ggacggtggc cgaacgcttc tcctacgatt acagcgacga agaaatcggc gcggtcgcgg
     1441 agcgggcgaa gaagctcgcg cgcgaggccg aggcggtgca cgttgttttt aacaacaacg
     1501 ccctcgactt cgcgccgcat gccgccttgc gcctgcgcgc ggcgctcggg cagatcacga
     1561 aaggtccgcc gcgagagggg gatttgtttc gttaggcgag caagaccatg gatcaaccac
     1621 cttccagcca gcccggcgtc tgggcacgac tccgtcgttc ggccagcggg ctccattttc
     1681 ggctcgtgac gatcgaggac acgccgcact cgattgcgct cggggtggcg atcggaatct
     1741 tctttggctt caccccgctc tggagtctta agacgctgct ttcgattgcg gtcgcgtggg
     1801 tctgcaagag caacaagatc gcggccgcga tcagcgtcca gttgcatgat ctcatcctgc
     1861 ccttcatgcc ggcgatctat ctttgggagt acaagtttgg ctattgggcg atgcgcggtc
     1921 atctgccgcg gcgagtgaat tttcgcacct tggaatttcg cgactacgtg cactgggaga
     1981 tgtttttttc agtcggtcgg ccgctcctca tcggatcgct catcatcggg ctgccctccg
     2041 cggcgctcgt ttatgttatt tgccgcgccc tggtcacaag tcatcgggcg acgcgcgctg
     2101 agccgtagaa ggcccagatt ttcgcggcga cggcacggga gttgcatcat ctcaacgaag
     2161 ttgccggctg ctgtcgtgga gacggaggac ggtgatctgc aaagccaaat tatgaaacct
     2221 cttatcgcta tcgcctccag cctgttgctt tgttcctgcg ccgatatggt cgtcacgaag
     2281 agtgccgtgt cgtcctcgtc gggtcgcgcc accgtcgagg ccaaggattt tgatccaaag
     2341 ggcggcgtgc attatgagac ctgcggggtg ggagcggcaa atccggctgc gatttacatc
     2401 cgcccatttt gcatcgattc cgccattttc cggggcgatg aggccccttc tgatggagaa
     2461 atgccgattc gcaaagcgct tgctcccgtc gcctttgcga atgatttgag agaagagctg
     2521 ggcaagatgg cgccgtcact cgttatcgcc gagaacgaag ttccgaccat tggctggctg
     2581 gtggaaggtc agttcgatct cgtcgatggg ggcgacccgc tcgctcgctt cttcttcggt
     2641 ccatttggcg cgggccaatc gatgctcgcc ttgcacgtca aggtgaccga ggtggcaacg
     2701 catcatgtta tttatgaatt cgacatggcc ggcggaagcc acgagcaggg gccactcggc
     2761 acggtccgcg ccagcggttt gggcaaagcg actcccttcg atctgcggaa tgcggccgag
     2821 cgtgtgctcg tggcgctcag ctcggatccc taccgttacg gcgcgcgggc gtcgctttca
     2881 cttcgttaag tggaaagcgc ccttcagagc gcgcggcgga tgattgcggt tgccaagcga
     2941 atcgcgccag ttaacatgca gcggttttga ccgccgtgat cgttttctct cgcaaaatcc
     3001 tgctcctcgc cgcggttctg gcgcttttct cgggcgcacc gagggccctg catggggccg
     3061 aggcgaccat cacggtcgac gccaaaaccg gcttgattct cgacaagctg cagtccgata
     3121 agaagcgcca ggtcggcagc ctgacgaaaa tcgcgaccgc gatggtcgtc ctcgattggg
     3181 ccgcgaaaga aagcggcgat ctcgggcagg tggcggtcat tcccgaagcc gcctttgccg
     3241 ggacgagcga aaacaatgtc ggttttcaac cgggcgacac ggtcacgcta cgcgacctgc
     3301 tctacgccgc gctcgtgcag tcggacaaca tcgcggccta caccctggcc gatcacgtgg
     3361 gccaggcgct gctgcggcgc gtcccgccga gcggggaggc ggcgcgcggc accccggtcg
     3421 attattttgt tctgcagatg aacgcgctcg cgcagcatct cgggatgatg cgcacgcgtt
     3481 tcctcaaccc gagcggtctc gacgcgaaag agaagccctt ctcgacggcc gccgacatcg
     3541 cccgactaac gagatacgcg atgaacaatg ccggttttcg tttttacgtc tcgcagaaag
     3601 aacgcggaat cgaaatcggc cgcggcgcag gccggctgcg ttatctgctg cggaacacga
     3661 acgaactgct tggtcgcgac gagatcgatg gggtcaagac cggtcgcacc ctgcgggccg
     3721 gagactgcgt agtgctttcc gcggcccggc cgtcggaagt ggtgcagcag ggcgcgacca
     3781 cgctcgtcac tccacggcgc cttatcgtgg tggtgctcgg tagtcccgat cgctttgcgg
     3841 aaggagcggc gctgctcgcc cgcggctggt cgctctacga tcgttgggcg gcggccggac
     3901 ggcccgtctc acccaagacg acgcttggcg gccctaacga gtaatgaaac cacggatcgg
     3961 cgtcctgacg agcggcgggg actgccccgg gctgaacgcg gtcttgcgtg gcgtggtcct
     4021 ggcggcggaa aagctgcgct gggaggtggt cgggttcaag gatggtttcg agggtctgct
     4081 cccgcccggc cattacatga tcctcgaccg cgcccgcacc gccggcatca ccccgttagg
     4141 cgggacgatt atcgggacga cgaataaagg gcattttgtg gccaaggtcg gcgccggcaa
     4201 tcgcgcgatc gtcgcgcgcg aagtgatcga gcaggcgcgt tccgttctcg gacatttgca
     4261 cgtcaccgcg ctgatcatcg ttggtggcga cggctcgatg acgaccgccc tgcagcttca
     4321 gggcgaggga atcaactgca tcggagttcc aaaaacgatc gacaacgatc tcgaggcgac
     4381 ggcgatgact ttcgggttcg attccgcggt cgcctgcgtg gccgacgccc tcgaccggtt
     4441 gcacaccacc gccaccagcc acaagcgcat catggtgctc gaagtgatgg gacggcacgc
     4501 gggttggatc gcgctgcacg gagggatcgc gggcggcgcc gatgcgatct tgatcccgga
     4561 gattcctttc gattacgaaa agttggcgga tttcatcaat cggcgcgagg cggcgggcag
     4621 tcggagttcg atggtggtgg tcgcggaagg tgccacgccg aaagacggcc agcaggttct
     4681 gcatcagacg gaagcggggg agtatcgctt tggcgggatt ggtgaggtgg tggcgcacga
     4741 acttgccgcc cgcacgggca aggacacgcg ctgctgcgtg cttggtcatc tccagcgcgg
     4801 cggcgcgccg accacgctcg atcgcattct cgggacgcgc ttcggagtca aggcggtgca
     4861 gctgatcaat gagggccact tcggttcgat ggtgagctac caaaactatc aggtgcagca
     4921 cgtgccgatc gccgatgccg tgaaccggtt gcgtctcgtc cagacctcga gccagatggt
     4981 ggagacagcg cgcgacgtgg agatttcctt cggggactaa atgtagccgt atccctaggg
     5041 gaaacgcggg acgatgggta gtttcgcggc aaaattctgc gagcgatgtt ttttcccgcg
     5101 tttcgcaaag cgaagcggct acagccgcgc gacctgctcg accgtcaact cgtcgagtcg
     5161 cctaacgacc aaatccgcgg cggcgagttg ctcgaccgga tgggtcgtcg gcacggccac
     5221 cactttcatg cgggcggcgt gggcggcttc gatacccaca tgggcatctt caaaaacgac
     5281 gcaacgctcc gccgccactc cgagccgggt ggccgctttc tggaaaacct ccggattcgg
     5341 ttttccatgc accacgtctt cggcggaaac gatttcacga aaagcgacct tcagcccgat
     5401 gcgctcgagc accgtgtcga tgttcaggcg ctgggtggac gatgccaccg cgcaagggac
     5461 gtccgcggcg cgcagccgct ccagccacgc cactactccc ggcaacggcg cgatctcgct
     5521 gcgcgcaacg atttcgcgat agagcgcttc tttgcgattc gttaggcgcg caatctccgc
     5581 gggctccttc gcccaaccgt gaatctcttc gatgatccgc gcgctcttca tcccgaagcc
     5641 gcggatgaaa ctgtccgcgg cgattgtttt tcccagctct tgcgcgagac gacgccagct
     5701 ctgctcatgc agcccggccg agtcgatgat gaccccgtcc cagtcgaaga tcgcgccgaa
     5761 gagcggtggc ccgctcgcgc tcacggcagc cgctgcagcg ccggcagacc gtaatttttt
     5821 tgcaaggcgt cggcttctgc cgccacctcg ctggcctcga gctcgagttg cttcgggtcg
     5881 tccgccgtct cgatcacgtg aaatccgttg accggcgttt ttaccgcctc ggccaggtcg
     5941 cctaacgagt tgatgacttt cccgttgacc ttcttgacca cgaggtaact gaagtcgtcg
     6001 taaccgatcg tgctgttggc cggcaggacc tggctcagga tgaccacgcg ccgcttccct
     6061 agggggaaaa gttccgactg aaacttatcg agataaacga aatcctgcgg cgcgtccttc
     6121 tgccagttgg cgccccattc cttgaggtat tgccgcgaga gctcctggaa aatcaggccg
     6181 ccgaggacgt agtagggcgg cgggtcgtcg atgttatagg gcggaacgac gtaatcctcc
     6241 tccgcgcggt gctcgagttt gacgttaatg gtaagcggtt tgcccgcgcg ttggacgtgc
     6301 agcgcgacgc tctcgccgac gtaggctttc ccggtgagca ggttgctgac ctcgagcttg
     6361 ccgtagagcg cgtcgacgta gtttccattt tcatccactg cgtggtctcc gaccgccgtc
     6421 acgatgtcgc ctcgctggag gcctgctttg tccgccggct gcccgggttc gacactcgta
     6481 acgtagatgc cgcccggctt gccggtcgca ccggcatagg cgcgcaactc cgggtcgcgt
     6541 gtcgggaaaa aggaaatgcc taacgacgga aaaccttcgt acttggggcc gctcgcgtcc
     6601 ttcaggaaat gcgcgatgat cggcgcggga atcgcgtcca gcgtttgcga gcgcggatcg
     6661 aagcgcagga ggagcgcggc cagcttgttg tttttcacca gcggcacggt gtagctgttg
     6721 tcgcggtact gcatcggaat gctcaggcga taggtgagaa aatcgctgac atcggccggg
     6781 tagcgggtca cgccgatcgt ggtcacgatt ccctccgtca cgaccagcgt gccggtcgct
     6841 tccagttgcc aggccgcgag gcgatcgccg accacggtgt cgagcgcgag atcgagcggc
     6901 tgcaaaccgt tgagaaattt cttatcgctc ggctcgagca gggcaaggtt cgcttcgtaa
     6961 tcgaccacca ccacattggc cgcggttttc tcgcccgatt ccgcgcgttc cagctcgacg
     7021 taattttggt tctcgaccag gtcggcggtc accagcaccc ggtcatgcgc caggacggcg
     7081 ccgagcgcgc gcttggaaaa cggcgccttc ttttgccacg gtcgaaagta gtcgaacggt
     7141 tgcccggtca cgttaactcg gacgagcgag acctccttgg cctgaaccgc cgcttgcgcc
     7201 gccatcgggg gcaaggtttt cacggggacc ggcgccttct tcgcctcagc cggcgcgaac
     7261 gcggcgagca gcgcaatcag gaaaagagca gtggtgcgca tgatcaactg ttggtttgcg
     7321 cggccaccgc gggtttggcg gggggctgtt catcgagctg ttgttcctgg aaaacgttgt
     7381 agcgcgtctt gatccgttcg cgcgccgcct ccacttgctt cgggtcgagg acgagaggcg
     7441 gaccgtcgcc gaccatcttc acgacaaagc ggtcagccgg ttgctcaaaa gcccgggcca
     7501 aatcctgtag cgtcccgatc ttctggccgt tgatttcatc cacgatgctg gaccgaaacg
     7561 gcagcagata ggtattgatc ggatcgggaa gaatattcgt gaggatgatg acctcggggt
     7621 gctgcaaata aatctgctcg gtcacgaaga aatcgaaaaa gtgtcgcacc cgcaggtcgc
     7681 cgatgcggta ggcctcgagc agatcgagcg acatcggttg aaagaggagt ccgccgtaaa
     7741 ggacgtagcg cgggcgaata tcgtagctgt gcccctgcat gaaataaggc caaaccgagc
     7801 ctaacgtgat caccacgctc atcggctgct ggttgcgcca gagatcgagc ttcaccgtgt
     7861 cgcccttgaa tttccgctcg accacctcgg gcatctgcac ccgttcgccc tcgtattcga
     7921 caaacgcgtc gcttgcgatc ggatacccat cgatcgaaag gagcacgtcg cccgacttca
     7981 gcagtttgcc gcacggaccg gcgtcaataa ccgtgccaac gagcacgccg cggtcgtcat
     8041 ctttcaaccc gagatagtgg cgctgggcgg gattttgcaa cttcaagtag gtgatcccga
     8101 gatcgacgta gcgatcgtag tggccgtcct cgatgtcttt cagaaagcgc cggatcaccg
     8161 gggtcgggat catgtaagcg acgccctggg cgatgtcgcc gctgtagccc tggaaagcga
     8221 cgccgaccac tttgcccgtc tgcatcaccg ggccgccgct gttgccggga ttgatctgcg
     8281 cgctgatctg aaccgcgagg tgcgaatcga tcgccgagtg agtgtagaga ttgaagtcga
     8341 tccgcgaaac gatcccggtc gtgaccgaca tgcgttcgcc gccgatcgga taaccgtaag
     8401 ccgagaccgt cgattcgagc gccgggatgc cgccgaagct gagcggcttc atgcctttcc
     8461 agaaattcgg cgcggccacc tgcaccgtcg cgagatcgca atcgtgcgcc acgaactgca
     8521 ccgtcgcggg atacttattc ggatcgccct cgcgctcgac cgtgaggtag cggctattgc
     8581 tcaccacgtg ggcgttggtc aggatgcgct ggccgtcgat cacgaacccg gcgccgaccc
     8641 cgcgcccgat cgagcccgag ttccacggcg cgcgataatc gggcgacacc tcggtcgagg
     8701 tgatgcggac gaggctgttt tggatctggc cgttgggctg tccgtgcgcc tccacgacga
     8761 ggaagagcgc gagcgcgcag atgagaaagc ggaagcggtg catcaggaaa agagcgggaa
     8821 gatcaaacag aagagcgcgg ctgtaaaacg acaatcgaag cgttagatgg ccggaagcca
     8881 aaaacgttca gagcaggtgc agtgccggga taatttgtag cggggggcgg tgacaccgcg
     8941 aggagacgcc atgacaatgc agatgccgaa cgaatgggat cgcacgcgtg gccgttgcat
     9001 cgagcaaggg acaagaaggg agctgtagcc gcttcgctgc gcgaagcgcg gaagagagac
     9061 cgg
//
LOCUS       DATMNS010000264        16863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3103932, whole genome shotgun sequence.
ACCESSION   DATMNS010000264 DATMNS010000000
VERSION     DATMNS010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 16863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16863
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3103932"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VNW28_09210"
     CDS             complement(<1..1074)
                     /locus_tag="VNW28_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10149.1"
                     /translation="MKKFAVSSLGAAVAAFLAMASPAKAGMLTVTSGPTFTPDAAFTN
                     NTIQLDTTVEGGLVHLIGGATVTPGATPAVTVDIGGDYSADAGDILSVAYSFTVDLNI
                     PTAVAYVISGTATVLGNPVNFSANGTLMPGLHKYEGTFQAPITFPVATSGTFSGKLEL
                     DFGTGAAATLAAAPGALDLSIQQIDFQLNPTAATIEPPSQPLNISTRLNVGTGDNVLI
                     GGFIITGTDPKLVVLRAIGPSLMVSGVPGVLADPFLELHDSTGAIVATNDNWMDNSTA
                     DQMVLTDHNLAPTNNLESALVETLNPGEYTAIVRGVGDTTGVALVEAYDLDDGTTNSV
                     LGNISTRGPVETANNVMIGGFILG"
     gene            1224..1451
                     /locus_tag="VNW28_09215"
     CDS             1224..1451
                     /locus_tag="VNW28_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10150.1"
                     /translation="MKMFLLIVLSVTALFIWQRHGGSDAAPESKPATATAAASATPRP
                     VYEHDWAKHSLDRAQEVANQVRETRKQNEQP"
     gene            1593..1946
                     /locus_tag="VNW28_09220"
     CDS             1593..1946
                     /locus_tag="VNW28_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HXA10151.1"
                     /translation="MNSPLFRIAAALCFLAVALGAFGAHALKGTLAANAMTDVWNKAV
                     LYHFVHALALLVLASLPAASRAPAAFFLSGIVLFSGSLYFLALTNIRWLGAITPLGGL
                     CFLAGWLSLVIWPPR"
     gene            2268..3770
                     /locus_tag="VNW28_09225"
     CDS             2268..3770
                     /locus_tag="VNW28_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HXA10152.1"
                     /translation="MLAFAETEQSAATIAVFIVTFLGTLALGRLLKRRAGVPFGIFFQ
                     LFALTLAFYTAAWVYGLELQWRNHAGAALVLLSTAVVIALVDRYLWDLYFEQGRQTVI
                     PKFLRDLVAGVIFLIALLLVLSVGYHAQSQLTWLLSGSGLAAIILAFATQNLLGGVIA
                     GMSVQINKPYKVGDWLQVGERFAEVMEINWRSSRLRTNDNIYLDIPNNEIVRTTIVNL
                     HYPTNLHAMRVRVGIDYNVPPNRVKDALFHAAMSAKNVLQDPPVRIFLVEFSEYSIIY
                     EIKFYMSDHSKINETNDSVRTNVWYELKRQNITIPFPIRTLEVNRKAVPKPHEQHGRA
                     RTILEGEPLFASLTEAQLDGLIRASRTLRFGRGEKVIDQGAEGSSMFVLLHGTAHVSV
                     AQNGAMIRVGSLRMGDSFGEMSLLTGERRSATVRAEDDCEVLEISKPAMAALLHDAPE
                     CVAQLSELLAARKMETEGLLKDAHESPAAETRKREYRATFLRRVRSVFEL"
     gene            3933..6008
                     /locus_tag="VNW28_09230"
     CDS             3933..6008
                     /locus_tag="VNW28_09230"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HXA10153.1"
                     /translation="MTLLALSFLSSGVSLSMLCAVAGLVFAFFLIFSVIKASPGNARM
                     QEIAGAIQEGAKAYLNRQVMTISAIAVIIFILLFIWKDHATAVGFVVGAACSLSAGFI
                     GMRIAVLANTRTTQAATTSRTAALRIAFNGGAVTGLLVVGLALLSVGIFYTVAENMIG
                     HDLAVKSLIGLALGASLISVFARLGGGIYTKAADVGADLVGKIENTLDEDDPRNPATI
                     ADNVGDNVGDCAGMAADVFETYAVSLIGAILVGALTLASTPAAIIYPFVLGGISVLGA
                     ICGILYVNVSGGKPGNVLMGGVMASALVSAVLFWPATHLLFPEGVVIANVARSANDLY
                     FASLVGLLMTGIVVVITNYYTSMNCGPVRKIAKASETGHATNIIAGLALGQHATALPV
                     GFIGLAILFSFHFAGLYGIAIAVMSMLSMAGIIISLDAFGPITDNAGGIAVMSHLPKE
                     IRGVTDELDAVGNTMKAVTKGYAIASAGLAALVLFGSYVEELRAHVTAAGRAFNFQFS
                     LTEPKVIIGLFIGGLLPFVFTAFSMDAVGKAAGAVVREVRRQLTAKPGILRGEDKPDY
                     GTCVDIVTKAALREMIIPALLPVVFVLAVAMIKPLGPVVLGGLLVGTIVTGLFVAIAM
                     TSSGGAWDNAKKFIEEGNLGGKGSFAHAASVTGDTVGDPYKDTAGPAINPMIKVVNIV
                     AILIIPIFF"
     gene            6032..6391
                     /locus_tag="VNW28_09235"
     CDS             6032..6391
                     /locus_tag="VNW28_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10154.1"
                     /translation="MDPQDFQLVPAPQREAPAKTDAGASPFKVVVDPQMRAELRRALI
                     DLIDNHDEALAAHYADGKLALESYKEYIEVFARSLKETMESREGVSYLLRAVGFDVPP
                     EEVNLRPDLRWKPGGQP"
     gene            6521..7798
                     /locus_tag="VNW28_09240"
     CDS             6521..7798
                     /locus_tag="VNW28_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA10155.1"
                     /translation="MSTVLLIEDDAESRRMTAELFSREEWTVIEAGDGEAGISLALEH
                     RPQLVLCDLLMPRVNGFQVCRSIRQQLQRTKIIIVSGRDYGVDRASAIEAGADEYLVK
                     PIFWENLREVIERVMPASADGPGTTGRVPDFMPPSTRLKFWGVRGSIPVPGSSTVGYG
                     GNTSCVEVRADGEIIILDAGSGMRELGIALEAEFGTRPIKLTLLITHTHWDHIQGLPF
                     FLPAYNGNNSMRLLGYAGAKEGLADILTRQMDPSVFPLSFRALPSTISIEEQKEMSFS
                     IGKVQVQACFVHHPGICVGYRLYTSKGSIAYLPDSEPYPAHAAAERAKLVEFLHRADV
                     LIIDAQYTDDEYQKKVGWGHGSLSSVVCLGLDAEVRKLFLFHHDPGRDDRALDEIVES
                     ARMIVLESGQPMEVDAAREGTEVWLGAHTARTP"
     gene            complement(7842..8369)
                     /locus_tag="VNW28_09245"
     CDS             complement(7842..8369)
                     /locus_tag="VNW28_09245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXA10156.1"
                     /translation="MPKYLVVSASGNPSSNSRKMGRIAFAHLEEARVDCRWLDLAGMD
                     LPLCDADACYAHPAAQKLSAAIAAVDGVIVAAPVYNYDVSASAKNMIELTGSAWEDKV
                     VGFLCAAGGMSSYMSVMAYANSLMLDFRCVIIPRFVFATGDAFDDDTLIDAEIGQRIE
                     EVAAELIRFTQALRG"
     gene            complement(8630..10087)
                     /gene="gatB"
                     /locus_tag="VNW28_09250"
     CDS             complement(8630..10087)
                     /gene="gatB"
                     /locus_tag="VNW28_09250"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXA10157.1"
                     /translation="MKYLTAIGLEVHVQLKTRSKMFCGCAVEFGAPPNSLTCPTCLGL
                     PGALPAMNHEALRLTTLTGLMLGCDFAPVCHFDRKNYFYPDMPKNYQISQYELPMCEN
                     GEVPLPELAYPRDAQKQIATPGKAVRLVRIHLEEDVAKSFHFETSTGIDFNRAGTPLM
                     EIVTQPEIDSPEEAFAFLTALKQILRSGAVSDADMEKGQLRCDCNISVRPAAQAVLGA
                     KIEIKNMNSISGVRRALAYEVERQTAALASGETLRQETRRWDDDAGQTFLMRTKEMAH
                     DYRYFPDPDLLPVRSEGLLREVRDRVPELPAAKRARFIAQYQVSSYDAGGLANDLDLA
                     RYFENAAQGARKPKQVANWILNDLQSALANNGLTITNSPVAPGALDELVNLIDDGQIS
                     NPQGKEVFAEMFASGKSASAIVQEKGIAQLSDPRAIEALCDQVIATYPKPVADFRAGH
                     AASLNYLKGQVIKLSQGKANPGLVGEILERKLKAV"
     gene            complement(10221..11084)
                     /locus_tag="VNW28_09255"
     CDS             complement(10221..11084)
                     /locus_tag="VNW28_09255"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HXA10158.1"
                     /translation="MTAGSSLWQSVFLALALLLVAFEIVRGWRLGVVRQTVRLLAVAC
                     AYAAAFFGGRLLLPVLRPFLRAPDLFISILAGAILALVVYAVINALGAILFKRTGQQS
                     AGVVRLVYGLGGGALGIFFGLFSVWLVVVAIRSVGALANAEVHAQAAARSPTALPQPS
                     PNNAPPMINSLAKLKNSIELGSLGEVVKAVDVVPGQTYQTLGKVGTIAANPQSAGRFL
                     SCPGARELTQNPKIVALRDDPEIMELIRHQRFLDLLQNPKLIDAMNDPALEADVRRFE
                     FQKALDYALKK"
     gene            complement(11081..12160)
                     /gene="aroC"
                     /locus_tag="VNW28_09260"
     CDS             complement(11081..12160)
                     /gene="aroC"
                     /locus_tag="VNW28_09260"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HXA10159.1"
                     /translation="MSSSFGRLFRITTFGESHGGGVGVVIDGCPPRISLGEADVQREL
                     DRRRPGQSALVSARQEADRCEILSGVFEGETLGTPIAILVRNEDARPEAYREMKETFR
                     PSHADFTYQAKYGIRNWQGGGRAAARETIGRVAAGAVAQKVLANLFPALEVVTYVTHV
                     YDIAAAVDSQTVTRAAVEANAVRCPDEVAAQKMTQAIEEARAGGDSLGGVIECVIRGA
                     PPGWGEPVFDKLEADLAKAMLSLPATKGFEIGAGFAAATMKGSEHNDPFEIRKDEVRT
                     ATNRSGGVQGGISNGENIIFRVAFKPTATIAREQKTVTVAKQAATLAGRGRHDPCVLP
                     RAVPMVDAMASLVLCDHALRQRSIS"
     gene            complement(12160..13323)
                     /locus_tag="VNW28_09265"
     CDS             complement(12160..13323)
                     /locus_tag="VNW28_09265"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019343 - cysteine biosynthetic process via
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HXA10160.1"
                     /translation="MKKKHHLATRVIHAGQSPDPSTGAVMTPIYATSTYVQESPGRHK
                     GYDYARSINPTRSAYERCVADLESGTRGWAFASGLAAMSTALETIDSGSHVLVSDDLY
                     GGTYRLFEKVRRRSANLDFTFIDMTDPSKLEAAIKPNTRMIWVETPSNPLLKLIDLES
                     VAKIARQHNAISVSDNTFASPWIQRPIELGFDMVIHSATKYLNGHSDMVGGVAVVGDN
                     PTLADSMQFLQNSIGAIAGPFDSFLALRGLKTLALRMERHCENALELATWLEQEPKVR
                     RTSYPGLTSHPQHDLAKRQMKGRFGGMVTLLLETDLAGTRRFLENTHLFSLAESLGGV
                     ESLVNYPPIMTHGAIPEAQRIALGITESLVRLSIGVEDVEDLRDDLQTALAAI"
     gene            complement(13413..14819)
                     /locus_tag="VNW28_09270"
     CDS             complement(13413..14819)
                     /locus_tag="VNW28_09270"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXA10161.1"
                     /translation="MTSPPLAQAVSPNKPPAILSLIGHTPLVEVTRLETGLCQLFVKL
                     ENENPTGSIKDRVALVMIEAAERAGQLKPGGTVIEATAGNTGLGLALIAGAKGYRIIL
                     VIPDKMSQEKIAHIRALGAEVRLTRSDVTRGHPEYYQDVAAKLAEEIPGAFYVNQFGN
                     PANPLAHEQTTGPEIWEQMNHDVDAVVVGVGSGGTLTGLGRFFKKVQPNLEMILADPT
                     GSILYEWVKTGKLERAGSWAVEGIGEDFIPDIADMSLVTDAFEIDDTESFAVARELLR
                     KEGILGGSSTGTLLAGALRYCRQQTKKKRVVTFICDTGNKYLSKMFNDFWMAEQGFVQ
                     RPTQGDLSDLISHRHEKGEVISVGPSDTLLTTFKRMRSADVSQLPVLNGAGRAVGIID
                     ESDLLVKVTRDPAKFNDLVQSAMTDRLETLSPAANISDLLGVFDRGRVAILMEGDKFL
                     GLITRTDLLSYLRLHMPK"
     gene            15215..15994
                     /locus_tag="VNW28_09275"
     CDS             15215..15994
                     /locus_tag="VNW28_09275"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXA10162.1"
                     /translation="MHRFPSLAGVLPAVLALSISVARAGDDPLQETTEEYFPLVRDGA
                     VTVENTDGSIHLYGWNEPRLRLSALCKAYTASRLHQIRVETTTRPAALTVHTIVPEVS
                     GIFADRSGTMDYTLTVPEMARLQLKLANGDVTLQGLRGGSARIELGNGRITALNCYAR
                     VQAHAVNGVLEIIYEWWENLPAAFDCALQQGKIAARLPGNARFAVDAATANGWIHNEF
                     RFHPPPRVDPGQSLRATTGPDAEVSLGLRTGRGNISLDALP"
     gene            complement(16004..>16863)
                     /locus_tag="VNW28_09280"
     CDS             complement(16004..>16863)
                     /locus_tag="VNW28_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10163.1"
                     /translation="RAICYRHLHNWEAALADLHRAEHLAPEDPLVWQQAVLFDRLGTF
                     LAEVRELDKDLAALPNDEELLGDRALLFLRAGDSALALDDATKAAQLAPTAVRPQLFR
                     ALAERTLGRTAELASSAVKGTLRLETLSPEFLQTIRRLDAEIIAEPGSADLRTNRAWQ
                     LNEIGQPQLALADAQSALQSDPKSAGACAEASYALEKLEREPEAYEQIKRATELDPNF
                     STAWQYRGELEMHRADYPEAIDSLTRALAINQTAAALAKREECYRKLGLLAKAQNDHK
                     TLEQFGAAR"
BASE COUNT         3108 a         5099 c         5187 g         3469 t
ORIGIN      
        1 gccgaggata aagccgccga tcatgacgtt gttggcggtt tcgacagggc cgcgggtgct
       61 gatgttgccg agcacggaat tggtcgtgcc gtcgtcgaga tcgtaggctt caaccaaggc
      121 tactccggta gtgtcgccca cgccgcgcac gatggccgta tactcacccg gattgagggt
      181 ttcgacgaga gcggactcga ggttgttagt cggtgccagg ttatggtcgg tgaggaccat
      241 ctggtcggcc gtgctgttat ccatccagtt gtcgtttgtc gccacgatcg ctccggtgga
      301 atcatgcaac tcgaggaagg ggtcggcgag cacgccgggg acgcccgaga ccatcaggga
      361 cgggccgatc gcgcgtagga cgaccagctt tggatccgtg ccggtaatga tgaagccgcc
      421 gatgaggaca ttgtcgcccg tcccgacgtt gagacgggtg gagatattca agggctgcga
      481 gggaggctcg atcgttgcgg ccgtcggatt gagctggaaa tcgatctgct ggatgctcag
      541 atccaaagcg cccggagcgg cggccaacgt cgccgcggct ccggtaccga agtccagttc
      601 cagcttgccg gagaaggtac cgctcgtagc gacgggaaaa gttatcggag cctggaacgt
      661 tccttcatac ttatgcaggc ccggcatcag ggtgccattg gcgctaaaat tcaccgggtt
      721 gcccagaacg gtcgccgtgc cagaaatgac gtaagcgacc gcagtcggga tgttcagatc
      781 gaccgtgaag ctgtaggcga cggacaaaat atcacccgcg tccgccgaat agtcgccacc
      841 gatatcgacg gtgacggccg gggtcgcgcc cggtgtgacg gtcgctccgc cgatcaggtg
      901 gaccaggcca ccctccaccg tggtgtcgag ctgaatggta ttgttggtaa atgcggcgtc
      961 cggcgtaaag gtcggacccg acgtgacggt gagcataccg gcctttgccg gactggccat
     1021 tgcgaggaat gctgcgacgg cagcgccaag tgaggatact gcgaattttt tcataatttg
     1081 atgagtgatt ggtttgcgtt ctttttcttt tccggggaat gcgtcaaaat atgattcgcg
     1141 tctgcgcgcg tggacggacg ccggggagtt ccggcacgtt attggatcgt ccaagtggcc
     1201 cgctggctgc tccctttcag ggcatgaaga tgtttctcct cattgtgctc agtgtcaccg
     1261 cgctctttat ctggcagcgt cacggcggca gcgatgccgc gcctgagagt aagccggcga
     1321 ccgcgaccgc tgccgcatca gccacgccgc gacccgtgta tgaacatgat tgggccaagc
     1381 actccctcga tcgcgcgcag gaagtggcga atcaagtgcg ggagacgcgc aagcaaaacg
     1441 agcagccgta ggaatttgaa tcgttgatcg tggggacggc tgaacggctc cttgcacgac
     1501 cgtcttgacg ttttgatgat tcccgcttcc aaagctggcg acaggagttg cccggcggtt
     1561 gcggtctccc gaatggcgga gcagcttcgc gcgtgaactc accgctcttc cggatcgccg
     1621 cggcactttg ttttctagcg gtggcgctgg gcgcgttcgg cgcgcacgcg ctcaagggaa
     1681 cgctggcggc caacgccatg accgacgtct ggaacaaggc ggtgctctac catttcgtgc
     1741 acgcgctcgc cctcctcgtc ctcgcctctt tgccggccgc gagtcgcgcg ccggcggcgt
     1801 tctttctcag tgggattgtt ctcttcagcg gcagccttta cttccttgcc ctaacgaata
     1861 taagatggct cggcgcgatc accccgctcg gcgggctttg ttttctcgcg ggctggttga
     1921 gcctcgtcat ttggcccccg cgatagaccc gaaacatatg aagtggttga aaatcttgtc
     1981 gagggccatg tggagggcgg ccgcggtcat tccaatctcg acaattgaat ttcaccgcat
     2041 ttgtttcgtt tattccaaca taatcagcga cgataattac tcggtagaaa aattcgctgt
     2101 atcgtatccc tcccctgcag ccgccgcctg gggccgccgc cgttatgccc cttggcggga
     2161 ctgaacattt gcgggaagaa attcgattcg ttcgttccag cacgttgtcc ggacgcgcac
     2221 cgggctttcc cgctgcttcg cactctgcta ttctacccgc ctaaccaatg ctggccttcg
     2281 ctgaaaccga acaatcggcc gccacgatcg cggtttttat cgtgacgttc ctcggcaccc
     2341 tcgcgctcgg gcggctcctc aagcggcggg ccggcgttcc ctttgggatt ttctttcagc
     2401 tctttgcact cactcttgct ttctatacgg cggcctgggt ctacgggctc gaactgcaat
     2461 ggcgcaacca cgcgggcgcg gcgctcgtcc tcctgagcac ggcggtggtg atcgcgctgg
     2521 tcgatcgtta tctctgggat ctttacttcg agcaaggccg ccagaccgtg attccgaagt
     2581 tcctccgcga cctggttgcg ggagtcattt tccttatcgc cctcctcctg gtcttgagtg
     2641 tcggctatca cgcgcagagc cagttgacgt ggctcctctc gggctcgggc ctggctgcca
     2701 tcatcctcgc ttttgcgacc caaaatctcc tcggcggcgt gatcgccggc atgtcggtgc
     2761 agatcaacaa gccctacaaa gtgggcgatt ggcttcaggt cggggagcgc tttgccgagg
     2821 tcatggagat caactggcgg tcgagccggc tgcggaccaa cgataacatt tacctcgata
     2881 ttccgaataa cgagatcgtt cgcacgacca tcgttaacct tcattatccg acgaatctgc
     2941 acgccatgcg cgtccgggta gggatcgatt acaacgtccc gccaaatcgg gtcaaggacg
     3001 cgctttttca cgcggccatg agcgcgaaga acgtgctcca ggatccaccg gtaaggatct
     3061 tcctggtcga gttcagcgaa tactccatta tctacgaaat caaattttac atgagtgatc
     3121 attccaagat caacgagacc aacgactcgg ttcgcaccaa tgtctggtac gaactcaagc
     3181 ggcagaacat caccatcccg ttcccgattc ggacgctcga agtcaaccgc aaggccgtgc
     3241 cgaagcccca cgaacagcat ggacgagcgc gcaccatcct cgagggagag ccgctcttcg
     3301 cttctctcac cgaagcgcag ctcgatggcc tgatccgcgc ttcccgcacg ttgcgtttcg
     3361 gacgcgggga aaaggtgatc gaccaaggtg cggaggggtc gtcgatgttt gtgcttttgc
     3421 acgggacggc gcacgtctcg gtcgcgcaaa atggagcgat gattcgcgtt ggatcgctgc
     3481 ggatgggcga ttcgtttggc gaaatgtccc tgctcacagg ggagcggcgc tcggcgacag
     3541 ttcgcgcgga ggacgactgc gaagtgctgg agatcagcaa gccggcgatg gcggcgttgc
     3601 tgcatgacgc tcccgaatgc gtggcacagc tcagcgaact cctcgcggcg cgcaagatgg
     3661 agacggaagg cttgctcaaa gacgcccacg aaagcccggc cgcggaaaca agaaaacggg
     3721 aatatcgagc gaccttcctg cggcgcgtgc gctctgtttt cgagctgtag aggctgccct
     3781 ctgggtggcg cgggcgaggc ttgctctgag agcgaggaaa tccgcgggcg ttccggcgcg
     3841 tcgcagagcg aagcgtctac agcgggcatc aaagcgtttg ccaccctaga aaacccttct
     3901 aaagtccagc actctttagt ccggcccgat ctatgactct attagccctg tcctttctca
     3961 gctccggcgt ctcgctttcc atgctttgcg cggtggccgg cctcgtcttc gctttttttc
     4021 tgatcttctc ggtgatcaag gcctcccctg gaaacgcccg catgcaggaa atcgctggcg
     4081 ccatccagga aggcgcgaag gcctatctca accggcaggt catgaccatc agtgcgatcg
     4141 ccgtgatcat tttcatcctc cttttcatct ggaaagacca cgccacggcc gtcggtttcg
     4201 tcgtcggcgc cgcctgctcg ctttcggccg gctttatcgg aatgcggatc gcggtgctcg
     4261 ccaatacccg gaccacgcag gcggcgacga cgtcgcggac ggctgcgcta cgaatcgcgt
     4321 ttaacggtgg cgcggtgacc ggtttgctgg tcgttggtct ggccctgctc tcggtcggaa
     4381 ttttctacac cgtcgccgag aacatgatcg gccacgacct cgcggtgaag agcctcattg
     4441 gcctggcgct aggggcgagt ctgatcagcg tcttcgcccg actgggtggc ggcatttaca
     4501 ccaaggcggc cgatgtcggc gcggatctcg tcggcaagat cgaaaacaca ctcgatgaag
     4561 atgatccgcg taatccggcg acaatcgccg ataatgtggg cgacaatgtc ggcgattgcg
     4621 cgggcatggc ggccgatgtt ttcgagactt acgcggtcag tttaatcggt gccatcctgg
     4681 tcggcgcatt gactctcgcg tcgaccccgg cggcgatcat ttatccgttc gtcctcggag
     4741 ggatctcggt cctgggcgca atttgcggca ttctttatgt caacgtcagc ggaggcaaac
     4801 cgggcaacgt cctgatgggc ggagtgatgg cgagtgctct tgtctcagcc gttcttttct
     4861 ggccggcgac ccaccttctt tttccggaag gcgtcgtgat cgcaaacgtt gcgcgctccg
     4921 cgaacgatct ttattttgcc tcgcttgtcg gcttgctcat gaccggcatc gtggtcgtga
     4981 tcacgaatta ttacacctcg atgaactgcg gtccggttcg caagatcgcg aaggcctctg
     5041 agaccggtca cgcgaccaat atcattgccg ggctcgcgct cggacaacac gcgaccgcgc
     5101 tgccggttgg ttttatcggt ctcgcgatcc tctttagttt ccattttgcc ggcctttacg
     5161 ggatcgcgat cgcggtcatg agcatgcttt cgatggcggg catcattatc tcactcgacg
     5221 ccttcggtcc gatcaccgat aacgcgggcg gcatcgcggt gatgagtcac ctgccgaagg
     5281 aaatacgcgg cgtgaccgat gagctggacg ccgtcggcaa cacgatgaaa gcggtcacga
     5341 aaggttacgc gattgcgtcc gccggtttgg cggcactggt cctcttcggt tcctacgttg
     5401 aggaattgcg cgcccatgtc acagcggcgg gacgggcctt caacttccag ttttcgctta
     5461 cggaaccgaa ggtgatcatt gggctcttta tcgggggttt gctgccgttt gtcttcaccg
     5521 ccttcagcat ggacgcggtc ggcaaagctg ccggcgcggt ggtgcgcgag gtgcgccggc
     5581 aattgaccgc gaagcccggc atcctgcgcg gcgaagacaa acccgattac ggcacctgtg
     5641 tcgatattgt gacgaaagcg gccttgcgcg agatgatcat tccggccctt ttgccggtgg
     5701 tgtttgttct ggcggtggcg atgatcaagc cgcttggtcc ggtggtctta gggggattgc
     5761 tggtcggcac gatcgtgacc ggcctgttcg tcgcgatcgc catgacttcg agcggcggcg
     5821 cctgggataa cgcgaagaaa tttatcgagg aaggcaatct cggcggcaag ggctcctttg
     5881 cgcacgccgc gtcggttacc ggcgataccg tgggtgatcc ctacaaggac acggccggcc
     5941 cggcgattaa cccgatgatc aaggtggtca atattgttgc catcctgatc attccgatct
     6001 tcttttagac ttcgcctaac gactgtccgt catggacccg caggattttc aactcgttcc
     6061 ggcaccgcag cgggaagctc cggccaaaac cgacgcgggc gcgagcccct tcaaggtcgt
     6121 ggtcgatccc caaatgcgtg cagagttgcg gcgggcgctg attgatctga tcgacaatca
     6181 cgacgaagcc ctcgcggccc attatgccga tggcaagctg gcgctcgaat cctacaagga
     6241 gtacatcgaa gtcttcgcgc ggagtttgaa ggaaacgatg gaaagccggg aaggcgtctc
     6301 ctatctcctg cgcgcggttg ggttcgacgt cccgccggaa gaggtcaacc tgcgaccgga
     6361 tttgcgttgg aagccaggcg gccaacctta aggaaaagca gattgccctt cggccaaacg
     6421 gagcccaggg aataccgtgc gccatcctcg acctgtcagg cagtgtgata aaacgcttgt
     6481 ttcccttccg gcactctgag aagaatcggc accggcaccc atgtctaccg tcttgttgat
     6541 cgaggatgac gccgaaagcc gtcggatgac cgcggaactt ttctcgcggg aagaatggac
     6601 ggtgatcgag gcgggcgacg gcgaggctgg aatcagcctt gctttggaac atcgaccaca
     6661 gttggtcctg tgcgatctgc tcatgccgag agtgaacggc ttccaggtct gtcgcagcat
     6721 ccggcagcaa ttacagcgca cgaagatcat catcgtttcc ggccgcgatt atggcgtcga
     6781 tcgcgccagc gcgatcgaag ccggagccga cgagtatctg gtgaagccga tcttttggga
     6841 aaacttgcgc gaagtcatcg agcgcgtgat gccggcgtcc gccgacggcc cggggacgac
     6901 cgggcgggtg ccggatttca tgccgccctc tactcgcctg aaattttggg gtgtgcgcgg
     6961 ctcgattccc gttcccggat catccaccgt gggctacggc gggaacacca gttgcgtcga
     7021 agtgcgggcc gatggcgaga tcatcatcct cgatgcgggc tccggcatgc gcgaattggg
     7081 gattgccttg gaagcagaat tcggaaccag gccaatcaag ctaacgcttc tcattacgca
     7141 cactcactgg gaccacatcc aaggcctgcc atttttcctc ccggcgtata acggtaataa
     7201 ctcgatgcgc ttgctcggat acgcgggagc gaaggagggg cttgcggaca tcctgaccag
     7261 gcagatggat ccgtcggtgt ttcccctcag ctttcgcgcc ctgccgagca ccatctcgat
     7321 cgaggagcag aaagaaatgt ctttctcgat cggaaaagtg caggtccagg cgtgcttcgt
     7381 ccatcatccg ggcatctgcg tcggctaccg tctttacact tccaagggtt cgattgccta
     7441 tctccccgat agcgagccct atccagccca tgccgcggcc gagcgcgcca agctggtcga
     7501 gttcctgcac cgggccgatg tcctgatcat cgatgcgcaa tacaccgacg acgaatacca
     7561 aaagaaagtc ggctggggac acggctcgct cagcagtgtc gtctgcctcg gactcgacgc
     7621 cgaggtgagg aaactcttcc tctttcatca tgacccgggt cgcgacgatc gggcgctgga
     7681 tgagatcgtg gaatcggcgc gcatgattgt cctggaaagc ggccaaccga tggaagtgga
     7741 cgcagcgcgc gaaggcaccg aagtctggct cggtgcgcac accgcgagaa cgccgtaggg
     7801 caagccaagc agccggcgcg ccccggcgcg aaatggccct gttacccgcg caacgcctgg
     7861 gtaaagcgaa tcaactcggc tgcgacctcc tcgattcgct gaccgatttc cgcgtcgatc
     7921 agagtgtcat catcaaacgc gtcgcccgtg gcaaagacaa agcgggggat gatcacgcaa
     7981 cgaaaatcga gcatcagact gttggcgtaa gccattactg acatgtagct gctcatccct
     8041 cccgccgcgc agagaaaacc aacaaccttg tcttcccacg cgctgcccgt cagttcaatc
     8101 atgttcttgg ccgatgcgct cacgtcatag ttatagaccg gcgcggccac gataactccg
     8161 tcgaccgcgg cgatggccgc gctcagtttt tgcgccgccg gatgcgcata acaggcatca
     8221 gcgtcacaca aaggcaggtc catcccggcg aggtccagcc accggcaatc aaccctcgcc
     8281 tcctcgagat gggcgaacgc gatgcggccc atcttacggc tgttgctgga agggtttccg
     8341 ctggcgctga ctaccaggta tttcggcatg agagtgagtt cgagagccag cgctgttttt
     8401 ttggctaaag aatctggccc accaccagta gcaccaagac aaggacggtg ataacgggca
     8461 cttcacctga gactatccgg cgtctttcgg acatttcctt atcgtttgct cgtggtggtc
     8521 gcagcgcaag cgtgaagcgg tggagcggcg gccgtgtcag ccgctacccc tgcggccgcg
     8581 tccctttgcg ggcgatattc ggaaagcttt cgcgagcaag cgcccaccac tacaccgcct
     8641 tcagtttgcg ctccaggatc tcgccgacga ggccgggatt cgccttgccc tgagaaagct
     8701 tgatcacctg gcccttgagg tagttgagcg acgcggcgtg cccggcgcgg aaatccgcga
     8761 ccggttttgg gtaggttgca atcacctgat cgcagagcgc ttcgatggcg cgcgggtcgc
     8821 tcaattgcgc gatccccttt tcctgcacga tcgcgcttgc ggacttgccg ctcgcgaaca
     8881 tttcggcgaa gacttccttc ccttgcgggt tactgatctg gccgtcgtcg atcaggttga
     8941 ccaactcatc gagcgcgccc ggggcgaccg gggaatttgt gatcgttagg ccgttgtttg
     9001 cgagagcact ttgcaggtcg ttcaggatcc agttggcgac ttgctttggc ttgcgggcac
     9061 cctgcgcggc gttttcaaaa taacgcgcca ggtcgaggtc attggccagc ccgcccgcgt
     9121 cgtaggaact cacctggtat tgcgcgatga accgcgcgcg ttttgctgcc ggcaactccg
     9181 gcacgcgatc acggacctct cggagcaagc cttcgctcct caccgggagc aggtcgggat
     9241 cgggaaagta acgatagtcg tgcgccatct ccttcgtgcg catgagaaag gtctggcccg
     9301 cgtcgtcgtc ccaacgccgc gtctcctggc ggagcgtctc cccactcgcc agcgccgcgg
     9361 tttgccgttc cacttcgtag gcgagcgcgc gtcggacgcc gctgatcgag ttcatgtttt
     9421 ttatttcgat cttcgcgccc agcaccgctt gcgccgccgg ccgcacggaa atgttgcagt
     9481 cacaacgcaa ttgacctttc tccatgtccg cgtcgctcac tgcgccggag cgcaggattt
     9541 gttttagcgc ggtgagaaag gcgaacgctt cctccggcga atcgatttcg ggttgcgtca
     9601 cgatctccat caagggtgtg ccggcccggt taaaatcgat cccggtgctc gtttcgaaat
     9661 gaaaactctt ggccacatcc tcttcgagat ggatgcgcac caggcgcacc gccttgcccg
     9721 gcgtcgcgat ctgcttctgg gcgtccctcg gataggcgag ctcgggcagc gggacttcac
     9781 cgttctcgca catgggaagc tcgtactgcg agatctgata attcttcggc atgtccgggt
     9841 agaaataatt cttgcgatcg aaatggcaga ctggcgcaaa gtcgcagccg agcatcaggc
     9901 cagtcagagt cgtcaggcgc agcgcctcgt ggttcatcgc cggcagagct cccggtaacc
     9961 cgaggcatgt cggacacgtc agggaattcg gcggcgcgcc aaactcgacc gcgcagccgc
    10021 aaaacatttt cgagcgcgtc ttgagctgga cgtggacctc gagtccgatg gccgtcaggt
    10081 atttcattcc ttaaaggaaa tctcgcacca cggcgacccg attgagcatc cgcgcgacga
    10141 ttgtcttgta gggcaagcgc tccgctcgcc aaagcaataa gggcaggcga ggcacccgtc
    10201 tccaatccgc tcctccgcgc tcatttcttg agagcgtaat ccagcgcctt ctggaattcg
    10261 aaccttcgca cgtccgcctc gagggccgga tcgttcatcg cgtcgatcag cttcggattc
    10321 tgcagcaaat cgaggaagcg ctgatgccgg atcagttcca tgatctccgg gtcgtcgcgc
    10381 agggcgacga tctttgggtt ttgtgtcagc tcccgggcgc ccggacaaga gaggaagcgc
    10441 cctgcgctct ggggattggc cgcgatcgtt ccgaccttgc cgagcgtctg gtaggtttgc
    10501 cccggcacca cgtcgaccgc cttaaccacc tcgcccaacg aaccgagctc gatcgaattt
    10561 ttcagcttcg ccagcgagtt gatcatgggc ggagcgttgt tcggcgaagg ctgcgggagc
    10621 gcggtcggac tgcgagcggc cgcttgggca tgcacttcgg cattggcgag cgctccaacg
    10681 gaacgaatcg cgaccacgac cagccagacg ctgaagagcc cgaagaaaat gcccaacgcc
    10741 ccgccgccga gcccgtaaac caaacgtacg accccggcgc tctgctggcc ggtgcgctta
    10801 aagagaatcg cgcccagagc gttgatcacc gcgtagacaa caagagcgag gatggccccg
    10861 gccaggatcg aaataaaaag atccggggcg cgcaggaatg gacgcaggac gggcaggagc
    10921 aaccggcctc cgaaaaacgc cgccgcgtaa gcgcaggcca ccgcgagcag acgaacggtt
    10981 tgcctaacga ctccgagtcg ccagccgcgc acgatctcaa acgcgaccaa gaggagggct
    11041 aaggcgagga agacgctctg ccagagtgag gaaccagcgg tcatgaaatg ctgcgttggc
    11101 gcaacgcgtg gtcgcagagc accagcgatg ccatggcatc gaccatcggc accgcccggg
    11161 gcagaacgca ggggtcgtgc cgcccgcgtc cggcgagagt ggccgcttgc ttcgcgacgg
    11221 tcacggtttt ctgttcgcgc gcgatcgtgg ccgtcggttt gaaggcgacg cgaaagatga
    11281 tgttctcccc gttgctgatc ccgccttgca cgccgcccga gcggttcgtc gccgtgcgga
    11341 cctcatcttt ccgaatctca aacggatcgt tgtgctccga tcctttcatc gtcgcggccg
    11401 caaaaccggc gccgatctcg aaacccttcg tcgccggcag gctcagcatc gccttggcca
    11461 gatcggcctc aagcttgtcg aagaccggtt cgccccaacc ggggggcgcg ccccggatga
    11521 cgcactcgat gactccgcct aacgaatcgc cgccggcccg ggcttcctcg atcgcctgcg
    11581 tcattttctg cgccgcaact tcatccgggc agcgcaccgc attcgcctcg acggcggcgc
    11641 gcgtcaccgt ctgggagtcc accgcggccg cgatgtcata tacgtgagtc acgtatgtca
    11701 ccacctcgag cgccggaaaa agattggcca ggaccttttg cgcgaccgcc ccggccgcga
    11761 cgcggccgat cgtttcgcgt gccgcagcgc ggccgccacc ctgccagttg cggattccat
    11821 atttggcctg gtaggtgaaa tcggcgtgcg agggacggaa ggtttctttc atttcgcgat
    11881 acgcttccgg tcgcgcatct tcgttgcgca ccaggatggc aatcggtgtg cctaacgttt
    11941 cgccttcaaa aaccccggaa aggatctcgc aacggtccgc ttcctggcgg gccgagacga
    12001 gcgcgctctg gcccggccgg cgccgatcga gttcccgctg gacgtcggct tcgcctaacg
    12061 aaattcgcgg cggacaacca tcgatcacca cgccgacgcc gccgccgtgc gactcgccga
    12121 aggtggtgat gcggaagagc cgcccgaaac tgctgctcat caaatcgccg ccagcgcggt
    12181 ctgcaaatcg tcacgcagat cctccacatc ttccacgccg atcgatagcc taacgagcga
    12241 ctcggtgatg ccaagcgcga ttcgctgcgc ttcaggaatc gcgccgtggg tcatgatcgg
    12301 cgggtagttg accaggcttt cgacgccgcc caggctttcc gcgagcgaga agagatgcgt
    12361 attctcgaga aaacggcggg tcccggcgag gtcggtctcg agcagcaagg taaccatgcc
    12421 gccgaagcgt cccttcatct gccgtttcgc gaggtcgtgt tgaggatgac tcgttaggcc
    12481 aggataactt gtccggcgga ccttcggttc ctgctccagc caggtggcga gttcgagcgc
    12541 attttcgcag tggcgctcca tgcgcaaggc gagtgtcttc aatccgcgca gggcgagaaa
    12601 actgtcgaac ggcccggcaa tcgcgccgat tgagttctgc aaaaattgca tgctgtccgc
    12661 gagggtcggg ttatcgccca cgaccgcgac cccgccaacc atgtcggagt ggccgttaag
    12721 gtatttcgta gcggaatgaa tcaccatgtc aaagccgagc tcgatcggtc gctgaatcca
    12781 cggactggcg aaagtgttgt cggaaaccga aattgcattg tgctggcgcg cgatcttcgc
    12841 gaccgactcg agatcgatca acttgagcag cggattactc ggcgtctcca cccagatcat
    12901 gcgggtgttt ggcttgatcg ccgcctcgag tttcgacggg tcggtcatat cgatgaaggt
    12961 gaaatcgaga ttcgcggagc gccggcgcac tttttcgaaa agccggtagg tgccgccgta
    13021 aagatcgtcg ctcaccagga catgcgagcc gctgtcgatc gtttcgagcg cggtggacat
    13081 ggcggcgaga ccggacgcga aggcccagcc gcgagtgccg ctttcgaggt cggcgacgca
    13141 tcgttcgtag gcgctgcggg tcgggttaat cgaacgcgcg tagtcgtagc ctttgtgccg
    13201 gcccgggctt tcctgcacgt acgtggaggt ggcgtaaatc ggtgtcatga cggcgccggt
    13261 cgagggatca ggcgattggc cggcatgaat gacgcgcgtg gcaaggtgat gttttttctt
    13321 catcaagacg aatctggaaa ccaggaagcc aggagaaacg gaagcgacaa cgacttcgtg
    13381 gattcctggc tttcagatca attttgttgg ttttatttcg gcatgtgcag ccggaggtag
    13441 gagaggagat cggtgcgcgt gatcaggccg aggaatttat cgccctccat caggatcgcg
    13501 acccgtccgc gatcgaatac gcctaacaag tcggagatat tcgcggccgg ggaaagcgtc
    13561 tccaggcgat cggtcatggc cgattgcacg aggtcgttaa acttggccgg atcgcgggtc
    13621 actttcacga ggagatccga ttcatcgata atcccgacgg cgcgcccggc gccgttgagc
    13681 accggcaact gcgagacgtc ggccgagcgc atgcgtttga atgtggtcag gagggtgtcg
    13741 ctaggcccga cggaaatgac ttctcctttt tcgtggcgat gcgagatcaa gtcgctcaaa
    13801 tcgccctgcg tcgggcgctg gacgaatccc tgttccgcca tccaaaaatc gttaaacatc
    13861 ttcgagagat atttgttgcc cgtgtcgcag atgaaggtga cgacccgttt ctttttcgtc
    13921 tgttgccggc agtagcggag cgcgccggcg aggagcgtgc cggtcgatga gccgcctaaa
    13981 attccctcct tgcgcaggag ctcgcgtgcc accgcgaagc tttccgtgtc atcgatttcg
    14041 aacgcgtccg tcacgagcga catgtccgcg atatcgggga tgaaatcctc gccgatccct
    14101 tcgaccgccc agctgccggc tctctccagt ttgccggtct tgacccactc gtacaaaatc
    14161 gagccggttg ggtcggccaa aatcatctcg agattcggtt gcaccttttt gaagaagcgg
    14221 ccgagaccag tcagtgtccc gccggagccg accccaacca cgaccgcatc gacgtcgtgg
    14281 ttcatttgtt cccaaatttc cgggccggtg gtttgttcgt gggcgagcgg gttggccggg
    14341 ttgccgaact ggttgacgta gaacgcaccg gggatttcct ccgcgagttt cgcggccaca
    14401 tcctgataat attccggatg tccgcgggtc acatccgatc gcgtcaggcg cacttcggcg
    14461 ccgagcgcgc ggatgtgggc gattttttcc tgggacatct tgtccgggat gacgaggatg
    14521 atgcggtaac cctttgcgcc tgcgatgaga gcgagcccga gaccggtgtt gcccgcggtg
    14581 gcctcgatca ccgtgccgcc cggcttcaat tgccctgcgc gctccgccgc ttcgatcatg
    14641 acgagcgcga cccggtcctt gatcgagccg gtcggatttt cattctccag cttgacgaaa
    14701 agttggcaca gcccggtctc cagccgcgtg acctcgacca ggggcgtatg gccgatgagc
    14761 gaaagaatcg caggcggttt gttaggcgaa acggcctggg cgagtggcgg actggtcatg
    14821 aagcaatggt gggtcgggcg gcagcgggcc ggcgaccaca gctatgcaac accttggctg
    14881 cgaatcgtca agcgctcgcc ggccgcggcg ttcggctccg cgagtcccaa gcccgggcac
    14941 catttgagtg gtggcccgtc cggcgctcga tgattggaaa tccgctttca ttgttgtgag
    15001 gattgattaa gacgcgcggc atggtgggtt ttcggtcctc atccggtcac tttcccagat
    15061 aaattattcg ccgacagaaa cttttgaatt ggcaaccgag actcgcaagc cgaccttggt
    15121 gtcgccgaaa agcaggcgag tcttcgattt gcagaagaga cattcgaggt caataggttg
    15181 cctgagctcc ctcgccgggt tcttgttcac ggcgttgcac cgttttcctt cgctcgccgg
    15241 agtcctaccc gctgtcctcg cgctctcgat atcggtggcg cgggcgggcg acgacccact
    15301 gcaggagact acggaggaat attttccgtt ggtccgcgat ggtgccgtga cggtcgaaaa
    15361 caccgacggg tcgatccacc tctacggctg gaatgagccg cgcctgcggc tgagcgcgct
    15421 gtgcaaggcc tacacggcct cccgcctcca tcagatccgc gtcgagacca cgacccggcc
    15481 cgcggcgctc accgtgcaca cgatcgttcc ggaggtatcc ggcattttcg ccgatcgctc
    15541 cggcacgatg gattacacgc tcacggttcc cgaaatggcg cgcctgcaat tgaagctcgc
    15601 aaacggcgac gttacgctgc agggcctgcg cggcgggagc gcccggatcg aactgggcaa
    15661 tggacggatc accgccctca actgctacgc gcgagtgcag gcccacgcgg tcaacggcgt
    15721 gctcgagatc atttacgagt ggtgggaaaa tcttcccgcg gctttcgatt gtgcgcttca
    15781 gcagggcaag atcgccgcgc ggcttcccgg caacgcgcgt ttcgcggtcg atgcggcgac
    15841 cgccaatggt tggatccaca acgaattccg atttcatccg cccccccgcg tcgaccccgg
    15901 ccagtcgctc agggcgacga ccggaccgga cgccgaggta tcgttaggct tgcgcaccgg
    15961 ccgcgggaac atcagcctcg acgcccttcc ttagcgcccg cgctcaacgc gcggcgccga
    16021 attgttccag cgttttgtgg tcgttctgcg ccttcgcgag gaggccgagt ttacggtagc
    16081 actcctcgcg cttcgccagc gcggccgctg tctggttgat cgccagcgcg cgggtcagcg
    16141 aatcgatcgc ctcgggataa tcggcccgat gcatttccag ctcgccgcga tactgccagg
    16201 cggtggaaaa attgggatcg agttccgtcg cgcgtttgat ttgttcatag gcttcaggtt
    16261 cgcgctctag tttttcgagt gcgtaactcg cttcggcgca ggcgccggct gatttcggat
    16321 ctgactggag cgcgctctgc gcatcggcca gcgcgagttg tggctggccg atttcattca
    16381 actgccaggc gcgattggtt cgcaaatccg cgcttcctgg ttccgcgata atttccgcat
    16441 cgagacgtct gatcgtctgc aggaattccg gcgagagcgt ctcgaggcgg agcgtgccct
    16501 tgaccgccga actggccagc tctgcggtgc ggccgagtgt ccgctccgcc agcgcgcgaa
    16561 acaactgggg ccggaccgca gtcggcgcga gttgcgcggc tttggtggcg tcatcgagcg
    16621 ccagcgcgga gtctccggcc cggaggaaaa gcagggcgcg gtcgcccagt aactcctcgt
    16681 cgttaggcag agccgccagg tccttgtcca gctcgcgcac ttccgcgaga aacgttccga
    16741 ggcggtcgaa gagcacggct tgttgccaga cgagcggatc ctccggggcg agatgttccg
    16801 cgcgatgcag atcggccagc gcagcctccc agttatgtag atgccggtaa cagatggcgc
    16861 ggt
//
LOCUS       DATMNS010000265         7956 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2038772, whole genome shotgun sequence.
ACCESSION   DATMNS010000265 DATMNS010000000
VERSION     DATMNS010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7956
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2038772"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VNW28_09285"
     CDS             complement(<1..222)
                     /locus_tag="VNW28_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10164.1"
                     /translation="MKSRFLTLLGLLAGAFATSALAGSEEGSYNPPNPVVEEPYKFSA
                     EFTVEQAYLGGADVQRGRRDVSDYDEYYSN"
     gene            complement(219..1211)
                     /locus_tag="VNW28_09290"
     CDS             complement(219..1211)
                     /locus_tag="VNW28_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10165.1"
                     /translation="MRSITLLRSFALALAFPVVVLAADPVTEMAQFSVFGQIDLAALA
                     RGEVKTAAGQPMSTARYLSVQSCFVIPNPPASVIDAMKRFDPTTHRELKVYLHTDLPA
                     APAAANFSKLNHPPDNSTVKALGAATAKMSPELQISRAEAQKYSAGQPVFAFWIDLLT
                     KRAQDFVAGGAAREAPYDHTSDPVQPGQELAALVRQQGRVDRQFGSFLGQTGLIGGKG
                     SLKPELYWELLAVEDDGVLTLGASCRRPTAGGGAQIAEGLYYASGGYNVSLTLYQLWP
                     VAIAGRASTLVWRGDFTSARSLGELHGIERLASEGAMRKDILKAVTAFQRDSSR"
     gene            1519..2472
                     /locus_tag="VNW28_09295"
     CDS             1519..2472
                     /locus_tag="VNW28_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA10166.1"
                     /translation="MNEPIISLAGLTKSYGNLCAVCDLSLQINRGEIFGFLGANGAGK
                     TTTMRMLCGLTRPSAGRATIEGADVWQKRRELRARYGYMAQKFSLYPDLTVLENLRFF
                     AGAYRVPRAKMEARIGRLLAQMDLQAKRNTAAGSLSGGMRQLLALGCALVHEPPLLFL
                     DEPTSGLDPVHRQQMWDLLYDLSHNGITIFVTTHYMDEAERCTEVGFLHEGRLLAKAS
                     PRALKGTFKAKLLELDATPAMDALVRLREAPGILGVSLRSGSLRIYAPEAETLLAEWQ
                     RQWPFPEFSLLAERWAEPDMEDVFTAYSQGYDAMLNKESVR"
     gene            2469..3656
                     /locus_tag="VNW28_09300"
     CDS             2469..3656
                     /locus_tag="VNW28_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA10167.1"
                     /translation="MRMRLSRQAIGSVAMKEFIHIYRDWRILVLLLILPPVFTLIFGH
                     AFENTKSSDVPALLENRDHSPESDQFVRQLLAETTFKWRMAQKIGGRPDLLREEVNAA
                     LIIPEGWGASLRNGDPMPLPLYLDGSDTNTASELEGRTQESLGVFQKKQLEKTVEGLP
                     DEVIDLAKKLPVAVRQEFVSAMSQWGLQTHVLYNPEERFIDYVVPGVIGIILQLLTVT
                     LTACTIARERESGTLYQLMVTSLRRGEIVIGKILPYLGISILLILVILAVAGWHFHVP
                     FHQPFVLGVICLLFLLCSLGLGLLISAFSRTQTQAIQLSVFFLLPVFILSGAFAPLEQ
                     LPLPIRYLSECFPLTHFCRAFRLVNLYRAGPGFYSGDLVFLFLGAIATFFGAALLLKR
                     IQE"
     gene            complement(3783..4697)
                     /locus_tag="VNW28_09305"
     CDS             complement(3783..4697)
                     /locus_tag="VNW28_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10168.1"
                     /translation="MKNFFPFVACCAVSFASLTRAGTEADDKSAVAATQATAQAVPLD
                     LFSIEQGYVFESDLNHGGSHGKQDEIQSEFEYGHRFHLEGDLYVHAGLAYNRYDFGST
                     RAPVPNHLQSLAGVIGLDLMHGTDVGAFIQVRPGFYTQNNFGISSFDAPITAGRIFTL
                     ETDKLYVFGGAYAAFLKGGYPVIPLVGVIWIPSEKVRLMGVLPEPKLIYSPTKKLDLW
                     IGGEIAGGSYRTDRNDAIRPTKLNGTQVDFSDYRTGVGLAYDLTNNCTLDLGAGYSIE
                     REFAFHRAGENYRTDPSPYLRIELKAAF"
     gene            complement(4886..5269)
                     /locus_tag="VNW28_09310"
     CDS             complement(4886..5269)
                     /locus_tag="VNW28_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXA10169.1"
                     /translation="MSKLTKRDIVVSISNQTGLVQHQVFDVVQRTLDQITDSLARNVA
                     VELRNFGVFQPRLTKPRVGRNPNQPGSSFVIPSRATVKFKAGKIMRQRVEKLSREMKE
                     AAENGNGRTSTKRSSDGPAAHAENN"
     gene            complement(5398..5832)
                     /locus_tag="VNW28_09315"
     CDS             complement(5398..5832)
                     /locus_tag="VNW28_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10170.1"
                     /translation="MKDTTLETQIRLLTLQVESWKKLHDFITYALDKSKPIISADQER
                     QFTEIRGILLQETEHVFNELSLVAELSGKAMNVLQRASSIRGVRELSVEETRRLESDW
                     NGVFTKIGVIQGQLKSRRRELLGRSALLQSLAGLFARRAAAR"
     gene            complement(5829..6569)
                     /locus_tag="VNW28_09320"
     CDS             complement(5829..6569)
                     /locus_tag="VNW28_09320"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HXA10171.1"
                     /translation="METRLLILNSDPVFQDRALAMLDAMRKVETRIVRTLSEAVYILI
                     REHFDLFVVEGAPSVAIEQATNTRQHFPSLEIVCVTDETNDATLQEQATRQKITVVIT
                     DCSNRRLRKQLQVIISAFERTTAAPSEGIDPCHSLIGNLNQFTAAEILQMSCLSQRSG
                     RFTFKSGRGRSEIYLHHGMVRHAIYDSLEGEAAVAEIFRWRQGRFYFEEGIISQVQTV
                     NRPWTHLLIDNLQKLDETLQLVSVQGQR"
     gene            complement(6668..7615)
                     /gene="rnhC"
                     /locus_tag="VNW28_09325"
     CDS             complement(6668..7615)
                     /gene="rnhC"
                     /locus_tag="VNW28_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HXA10172.1"
                     /translation="MNSYTAPLTHEQASKLQALLRELGFDFSPKPYTLFFAQKNKLSV
                     AVYEKGPKVLLQGKGIEDFVSFELEPKILGEARLGYEEVHSPEMFEPHFGIDESGKGD
                     FFGPLVIAGVFTDRAIARGFLENGIQDSKRIGSDAKIRALAKVIRQTPGIAFDVVLIG
                     PERYNDLYEKFGNLNSLLGWGHARVIENLLAKRPDCPRALSDKFADARVIEKALLQRG
                     QRIQLEQRTKAESDFAVAAASILAREGFIDWLERAGKRLGVKLGRGVSGAVKETARQI
                     VEKEDPAALRRFAKIHFRTAHEIAPEHYAAPPPRRAWRK"
BASE COUNT         1479 a         2355 c         2480 g         1642 t
ORIGIN      
        1 gttggaataa tattcgtcgt aatcgctgac gtcccgtcgg ccacgctgca catcagcgcc
       61 gccgagatag gcctgttcga cggtgaattc ggcggaaaat ttatacggtt cttccacgac
      121 cggattcggc gggttgtaag aaccctcctc gcttcccgcg agggcggaag tggcaaacgc
      181 gccggcgagc aaaccgagga gggtgagaaa acgggatttc atcggctact atcgcgttga
      241 aaggcggtga ctgccttcaa aatatccttc cgcatggctc cttccgaagc cagacgctcg
      301 ataccgtgca actcacctaa cgatctcgcg gaggtgaaat cgccgcgcca gacgagggtc
      361 gaagcgcggc cggcgatcgc gaccggccag agctggtaga gggtgagaga tacgttgtag
      421 cccccgctcg cgtagtagag gccctcggcg atctgcgcgc cgccgccagc cgtcggccgg
      481 cggcaggaag cgccgagggt gagcacgccg tcgtcctcca ccgccagcag ttcccagtag
      541 agctcgggtt tgagcgagcc cttgccgcca atcaaacctg tctggccaag gaaactgccg
      601 aactgccggt cgactctgcc ttgttgccta acgagcgcgg cgagttcctg gcccggctgg
      661 accgggtcgc tggtatggtc gtagggcgcc tcgcgcgccg cgccgcccgc gacgaagtcc
      721 tgcgcacgct tcgtcaggag atcgatccag aaagcgaaaa ccggctggcc ggcggaatat
      781 ttttgagctt cggcgcggct gatttgcagt tcgggcgaca tcttcgcggt cgcggcgccc
      841 agcgccttca ccgtggagtt gtcgggtgga tgattgagct tcgaaaaatt ggccgcggca
      901 ggcgccgccg gcagatcggt gtggaggtag accttcaatt cgcgatgcgt cgtcggatcg
      961 aagcgtttca tcgcgtcgat tacgctggcg ggcgggtttg gaatgacgaa acaactctgg
     1021 accgacagat agcgcgccgt gctcattggc tggccggcgg ccgttttgac ctcgcccctg
     1081 gcgagcgcgg cgaggtcgat ttggccgaag acggagaact gcgccatctc ggtaaccggg
     1141 tcggcggcca gaacgacgac gggaaaagcg agggcgagag cgaaggatcg aagcagggtg
     1201 atggatctca tggcgttagg gaaagtttcg aaacggcgag cgcagctacg ggcattagtc
     1261 ttgagcggac gggcaggctg tcaatgagcg gcggcgtgtt gtgttgtggg ccaagttgta
     1321 gccgtgggcg gggatcccgg ataggcgagg tgtcgcggct cgcatccgcc gagggcagcg
     1381 cccccggcta cagaaaggcg cggaataact cgatcggggg cgcacttatc tgaatgccat
     1441 ttcgggcctg gccttgaaaa gagctccgtt ttccctagtg agaaagcgga tggacgcaag
     1501 ggtgcgtccc ttgcccggat gaacgagcca atcatttctc ttgccggcct aacgaagagc
     1561 tacggcaacc tctgcgcggt gtgcgatctt tcgctccaga tcaatcgcgg cgagattttc
     1621 ggcttcctcg gcgccaatgg cgcgggcaag acgacaacga tgcgaatgct ttgcgggctg
     1681 acccgcccgt cggcgggaag agcgacgatc gagggcgccg atgtctggca gaaacggcgt
     1741 gagctgcgcg cgaggtatgg ctacatggcg cagaagttca gtctctatcc ggacctgacg
     1801 gtgctcgaga atctgcgttt ttttgccggc gcctaccggg ttccaagggc caaaatggag
     1861 gcgcggatcg ggcgactgct cgcgcagatg gacctgcagg caaagcgcaa cacggcggcc
     1921 ggcagtctgt cgggcgggat gcggcaactg cttgcgctgg gctgcgcgct ggtccacgaa
     1981 ccgccgctgc tttttctcga tgagcccacc tccgggctgg acccggtgca ccggcaacag
     2041 atgtgggatc tgctctacga tctgagtcac aacggcatca cgattttcgt cacgacgcat
     2101 tacatggatg aagccgagcg ctgcaccgag gtgggttttc tgcatgaggg ccgcctcctg
     2161 gcgaaggcgt cgccccgcgc gctgaagggc actttcaagg cgaagctgct cgaactcgac
     2221 gccacgccgg cgatggatgc gctcgttagg ctgcgcgaag ctccggggat cctcggggtg
     2281 tcgttgcgca gcgggagtct gcgcatctac gcgccggaag cggagaccct cctcgcggaa
     2341 tggcaacggc aatggccttt ccccgagttc agcctgctgg ccgaacgctg ggcggagccg
     2401 gacatggagg atgtctttac cgcttattcg caaggttacg acgcgatgct gaacaaggaa
     2461 tccgtccgat gagaatgcgg ttatccaggc aggcgatcgg cagcgtggcg atgaaggagt
     2521 tcatccacat ctaccgcgac tggcgcatcc tcgtcctttt gctcattttg cccccggtct
     2581 tcacgctcat ttttggacac gccttcgaga atacgaaatc aagcgacgtg cccgccctgc
     2641 tggagaaccg cgaccactcg ccggagagcg atcaattcgt caggcaactg ctcgccgaaa
     2701 cgaccttcaa atggaggatg gcgcagaaga tcggcggccg cccggatctg ctgcgggagg
     2761 aagtgaacgc cgcgctcatt attcccgagg gttggggcgc gagcttgagg aatggcgatc
     2821 cgatgcctct cccgctctac ctcgatggga gcgacacgaa caccgcgagc gaattggaag
     2881 gccgcaccca ggaaagtctc ggtgtcttcc aaaaaaagca gctcgagaag acggtcgaag
     2941 ggttacccga cgaggtgatc gatctcgcga aaaagttgcc ggtcgcggtc cgccaggagt
     3001 tcgtctcggc gatgagccag tggggactcc agacccacgt gctctacaat ccggaggagc
     3061 gcttcatcga ctacgtggtg ccgggcgtga tcggaatcat cctgcaattg ctcaccgtca
     3121 ccctgacggc ctgcaccatc gcccgcgaac gggaatccgg cacgctctat caattgatgg
     3181 tcacctcact ccggcgcggc gagatcgtga tcggaaagat tctgccctat ctcgggattt
     3241 cgattctcct cattcttgtc atcctcgccg tcgcgggctg gcattttcat gtcccttttc
     3301 atcaaccctt cgtcctcggg gtgatttgcc tcctcttttt gctctgctcg ttagggctcg
     3361 gtctcctcat ctccgctttt tcgcggactc agacgcaggc gattcagctt tcggttttct
     3421 ttttgctgcc ggtttttatc ctctcgggag cattcgctcc gctggagcag ttgcccctcc
     3481 cgatccgcta cctctcggag tgctttccgc tcacccactt ttgccgcgcc ttccgcctgg
     3541 tgaatcttta tcgggccgga cccgggttct acagcggcga tctcgttttt ctatttctcg
     3601 gggcgatcgc cactttcttc ggcgccgcgc tgctcctcaa gcgcattcag gaatagcgag
     3661 cggcgtcagc gcggttgcga tctcggcgcg gaagaccctg atggtgtcga gtgtttcctg
     3721 cctccgccga gcgcctggcc taacgaacgc ggcaacgtct tctggaaagc ggaaaggcca
     3781 actcagaatg cagcttttaa ctcgatccgc aggtaggggg aaggatcagt gcgatagttt
     3841 tcgccggcgc gatgaaaggc gaactcccgc tcgatcgaat agcccgcgcc caggtcgagc
     3901 gtgcagttat tcgtcaagtc gtaggccagg ccgacgcccg tccgataatc gctgaaatca
     3961 acctgcgtcc cgttgagctt ggtcggccgg atagcgtcgt tgcgatcggt ccggtaggag
     4021 ccgccggcga tttcaccccc gatccagaga tcgagctttt tcgtggggga ataaatcagc
     4081 ttgggttccg gaagcacacc catcaggcgc actttctcgc tcggaatcca gatcacgccg
     4141 accagcggta tgacggggta gccgcctttg aggaaagcgg cgtaggcgcc gccaaaaacg
     4201 tagagcttgt ccgtctccag ggtgaagatc cggccggccg tgatcggcgc gtcgaaggac
     4261 gagatgccga aattgttctg ggtgtaaaac ccgggccgca cctggatgaa ggcgccgaca
     4321 tcggtcccgt gcatgaggtc gagaccaatc actccggcga gcgattggag atgattgggg
     4381 acgggcgctc gggtgctccc gaaatcgtag cggttgtagg ccaaacccgc gtgcacgtag
     4441 agatcgccct ccagatggaa gcgatggccg tactcgaact cgctctggat ttcgtcctgc
     4501 ttcccatggc tgccgccgtg attcagatcg ctctcaaaaa cataaccctg ttcaatcgag
     4561 aacaggtcga gcgggactgc ctgggcggtg gcttgggtgg cggcaacggc tgacttgtca
     4621 tccgcctccg tccccgcccg ggtgagcgaa gcgaacgaaa cggcgcagca ggcaacgaaa
     4681 ggaaaaaaat tcttcatcgg atcgggttgt cttagctcaa tcgcgcgatg ggcacaaaat
     4741 ggattcgcga tcggcccaaa tctttttgcg aatttcgagc cgggaagtta ttgcgaacct
     4801 tcctggtgcc gccgtcagcg gagcctgccg ggagcgcaac cgcggttcat gcgactgcga
     4861 ttccggggag gacacttcgc atccctcaat tgttctcagc gtgcgcggcc gggccgtcgg
     4921 aactacgctt cgtcgaagtg cggccattcc cgttctccgc cgcttccttc atctcgcggg
     4981 agagcttttc gacgcgctga cgcatgattt tccccgcctt gaatttcacc gtcgcccgcg
     5041 agggaatcac gaaactgctc cccggctggt tggggttgcg accgacgcgc ggcttggtca
     5101 gccgtggctg aaagacgccg aagttgcgca attccaccgc gacgttccgc gccaggctat
     5161 cggtgatttg atccagcgta cgctggacca cgtcgaaaac ctgatgctgc accagacccg
     5221 tttggttgct gatcgagaca acaatgtcgc gctttgtgag tttactcatg gtgtaccaga
     5281 atcgtgtatc gaacatcagg tgggcgggtg gtgtgcgcaa taagtttatc gcgcccgccg
     5341 cggcagacgc cacggccgaa agcatttctg tcgatctggg ttccgcggag ggagcggcta
     5401 gcgcgcggct gctcgccggg cgaagagacc cgccagagac tgcaggagcg cgctgcgccc
     5461 gaggagttct cgccggcgcg atttcagttg tccttgaatc accccgatct tggtgaagac
     5521 gccgttccaa tccgactcga ggcggcgggt ctcctcgacg gagagctcgc gcacgccgcg
     5581 gatcgacgag gctctttgca ggacgttcat cgccttgccg gaaagttccg ccacgaggga
     5641 gagttcgttg aagacgtgct cggtctcctg gagaaggatt ccgcggatct cggtgaattg
     5701 acgttcctga tccgccgaga tgatcggctt cgacttgtcg agcgcgtaag tgatgaaatc
     5761 gtgcaatttc ttccagcttt cgacctgaag tgtgagcaac cggatttggg tttcgagtgt
     5821 ggtgtctttc atcgttgtcc ttgaacgctg acgagctgca gggtctcatc gagtttttgc
     5881 aggttatcga tcagcaagtg cgtccatggt cgattgaccg tctgcacctg gctgatgatg
     5941 ccttcttcaa agtagaagcg gccctggcgc cagcggaaga tttcggccac ggcggcctcg
     6001 ccttcgagcg agtcgtaaat cgcgtgccgg accatcccgt gatgcaggta gatttcgctg
     6061 cggccacggc ccgacttgaa agtgaaacgg ccgcttcgct ggctcaggca gctcatctgg
     6121 aggatttcgg cggccgtgaa ttggttgaga ttgccgatca acgagtggca gggatcgatc
     6181 ccttcggacg gtgccgcggt ggttctttca aaagccgaga tgatcacttg cagctgcttg
     6241 cgcagccggc ggttactgca gtcggtgatg accaccgtga ttttctgccg cgtggcctgc
     6301 tcttgcaggg ttgcgtcatt ggtctcatcc gttacgcaga cgatctcgag cgaagggaag
     6361 tgctggcggg tattggtcgc ctgctcgatc gcaaccgacg gcgcgccttc caccacaaac
     6421 aggtcaaagt gttcgcggat caggatgtag accgcttcgg aaagcgtgcg gacgattcgg
     6481 gtttcgactt tgcgcatggc gtcgagcatc gccagcgccc ggtcttggaa cacgggatcg
     6541 gagttgagga tgagcagtct tgtttccacg ccgttacaat ggagcgaatc acgtaaaaag
     6601 caggaagctt gccgcgaccc gttgggacag gaggcgattt tcacggggtg aacccgaagc
     6661 gcgaacgtta ctttcgccac gcccgtcgcg ggggaggcgc ggcgtagtgt tcgggcgcga
     6721 tttcgtgcgc ggtgcggaaa tggattttcg cgaatcgccg cagcgccgcc ggatcttcct
     6781 tttcgacgat ctgacgcgct gtctccttga ccgcgccgga gacgccgcgc ccgagcttca
     6841 caccaaggcg cttcccggcg cgctccagcc agtcgatgaa cccttcgcgc gccagaattg
     6901 acgccgccgc gaccgcgaaa tccgattccg cctttgtccg ttgctcgagc tgaatccgct
     6961 gcccgcgttg caagagcgct ttctcgatca cgcgcgcatc ggcaaactta tcggataacg
     7021 cccgcggaca atccgggcgt ttcgcgagca ggttttcgat gacccgggcg tgaccccagc
     7081 cgagaaggct gttgagattg ccgaatttct cgtaaagatc gttgtagcgc tccgggccga
     7141 tgagcacgac atcgaacgcg atcccgggcg tctgccggat gacctttgcc agcgcccgga
     7201 ttttcgcgtc cgacccgatc cgcttgctgt cctggattcc gttttcgagg aacccccgcg
     7261 cgatggcgcg atcggtaaac acgccggcga tcaccagcgg cccgaagaag tcgcccttcc
     7321 cgctttcgtc gatgccaaaa tgcggctcga acatttccgg cgaatgcacc tcctcgtaac
     7381 cgaggcgtgc ttcgcccaga atcttcggct ccagttcgaa actgacgaaa tcctcgatcc
     7441 ccttgccttg caggagcacc ttgggcccct tttcgtaaac tgcgacgctc agtttattct
     7501 tctgcgcgaa aaaaagcgtg tagggtttcg gcgaaaaatc gaaaccaagc tcgcgcagga
     7561 gggcctggag cttcgatgct tgttcgtgcg taagaggagc ggtatacgag ttcactttgc
     7621 ggaaaatttc atgctgcgcc gcgcccaaca cctctctcca taacgggaca acgccatctt
     7681 ggaaatgatc aaccgccccg cttttcgatc cgccttgcaa cgctggtatg cggttcacgg
     7741 tcgcgacctg ccgtggcgcc ggtcgcgcga tccttatgcg attttagttt cggaattcat
     7801 gcttcagcaa acccaggtcg cgaccgttct tccttactat cggcgctggc tcgagcgttt
     7861 ccctgattgc ggcgcgctcg cgcgcgcaaa agaatcggag gtcctgcacg cctggcaggg
     7921 acttggctat tatgcccgcg cccgcaatct ccacgc
//
LOCUS       DATMNS010000266         7960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3792376, whole genome shotgun sequence.
ACCESSION   DATMNS010000266 DATMNS010000000
VERSION     DATMNS010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7960
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3792376"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /gene="rsmA"
                     /locus_tag="VNW28_09330"
     CDS             complement(<1..999)
                     /gene="rsmA"
                     /locus_tag="VNW28_09330"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HXA10173.1"
                     /translation="MKLSEIGNILREIRVAPVKSLGQNFLHDQNLARWIVERAELSAT
                     DFVLEVGPGLGAMTEPALATGAEILAIEKDGRLADFLRTHFANRRLQVIHGDALDFDT
                     RQLFQHGPVKLLGNLPYYVSTQILLRFLAQPNPIILALLMLQKEVAARLSALPRSKDY
                     GNLTLVVQSQYRVDYLRTVSSSVFLPRPEVDSALVRLLPRVPGELPPHDHELFVRLVR
                     RGFSQRRKQLGKLLQKELADWPGAAAEIGAAVQARAEELSLEQWIALTNLVQPAGPPA
                     AGTDPKEQFAVVDKNDRVTGAAPRGEVHGNNLRHRAIHIFLFNCAGELLLQKRSRWK"
     gene            complement(1166..2278)
                     /locus_tag="VNW28_09335"
     CDS             complement(1166..2278)
                     /locus_tag="VNW28_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10174.1"
                     /translation="MNNSVLLHIVERLETFLGRLPDSIRKPVLHELTPLKELFLQQRP
                     PRFVLTGSDPMPLLDFVARIFSDGAAETPARVTLTGLYRWQKIMIGQHGSIQLLDARG
                     ADEAALALVREELRGQPADLFFFLPAERDAKVTRQHEMERLEKLLAWNIDFKAGAKLI
                     ELCANDTESRSLPDETTSTPAIRASLIGVHSLSGTVRSTLARELPNEARVEMVRIGGD
                     RQAQTEIAELLVKSTTAICTAVGAQPIPLADLPILTTLQLVMVSGIMYISGRERSMRA
                     ATEFVGALGANVGFGMILREGTRAALKFFPGWGNVVCGMVAGAGTFAIGRAATVYFVE
                     GATLRDARRAFAQNKKKSLARPAKKPKSLREPSSKP"
     gene            2508..3827
                     /gene="ffh"
                     /locus_tag="VNW28_09340"
     CDS             2508..3827
                     /gene="ffh"
                     /locus_tag="VNW28_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXA10175.1"
                     /translation="MFSQLGDKLQDIFKDLRGHGSISETNISDAMRQVRLALLEADVD
                     YQVAKNFVARIKEKALGEEVLRSVTPGQQVVKIFHDELTALLGGDAAPLQLDKPARIL
                     MIGLNGAGKTTSSAKLALYLKKQGRVPLLIACDLQRPAAIEQLATLGGQISVPVYAPS
                     PDEKNVKRAAAASLEWAEGQNGNVQIFDTAGRQEIDTALIEEIKQLKEFLKPQEVLLV
                     ADAATGQQAVSVATHFHEALGITGIVLTKLDGDARGGAALSMREVTQRPIKFAGVGEK
                     LDQFEPFLPDRLAGRILGMGDIVGLVEKAAEAIDEEEATRMEQKLRTASFDFNDFLAQ
                     FKMMRKLGPLESILGMLPGVGNMKDLNIDENQLKRTEAIVLSMTDHERRRPDILNARR
                     RQRIARGSGSTVTEVNALLQRFNQMRKMMKNAGKMKRMMAQMSKMKS"
     gene            3928..4218
                     /gene="rpsP"
                     /locus_tag="VNW28_09345"
     CDS             3928..4218
                     /gene="rpsP"
                     /locus_tag="VNW28_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HXA10176.1"
                     /translation="MAVSIRLRREGAKNRPYYKVVVADSRSPRDGKFIEIIGTYDPKM
                     QGENSTLKVDRAEHWIARGAQPSDTVRSLLKKNKARAAAAPAEPVAAEAAAE"
     gene            4313..4543
                     /locus_tag="VNW28_09350"
     CDS             4313..4543
                     /locus_tag="VNW28_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HXA10177.1"
                     /translation="MRQFLEFIIRQLVEFPEEVMLSEIPNGRMTVFRLQMRQTDVGRI
                     IGRNGQTIRAIRALLVSAAARHGEKATLDIVE"
     gene            complement(4664..5659)
                     /locus_tag="VNW28_09355"
     CDS             complement(4664..5659)
                     /locus_tag="VNW28_09355"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HXA10178.1"
                     /translation="MLRLHLAIWLGGFVLAGTSAAQQKNNPMKFGMVIHGGAGTIERS
                     EMTPENEAAHRAGLEQSLKAGYAVLERGGSSLDAVEAAIRVLEDNPLFNAGKGAVFTH
                     EGTNELDASIMDGKTLNAGAVADLQHIRNPISLARMVMEKSPHVMLVGDGAEAFARKM
                     GMPFVDQKYFYTNERWQSLQKEKAKTSPAPMSDKDRHGTVGAVALDKAGNLAAGTSTG
                     GTTNKQSGRVGDSPIIGAGTYADNRTCAVSCTGDGEYFIRSVVAHDISAMMEYKGMPV
                     QAAAQAALDKAGRLGGTGGLIAIDKQGNFAMPFNTSGMYRGRVGEDGKVNVEIYR"
     gene            5763..6617
                     /locus_tag="VNW28_09360"
     CDS             5763..6617
                     /locus_tag="VNW28_09360"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA10179.1"
                     /translation="MPDNASPTPGTAGTIVHAPGDLLAGVGEKSRSLLLEVGSMFWFI
                     INTIGETLDRMRRGRVPFRAASFFRYTERAGVGSVPLVAMVSFFLGLTMALLTGYQLE
                     RFGTERLVPGLVAISFTRELGPLLTGIMLAARIGASYTAELGTMQVSEEVEAIEAMGI
                     GPLRFLVAPRLLALFALMPCLSVISSVAAIFASALISRAYFSIAFVYFQDLVLQSLLI
                     RDIVTGILKSFMFGLLIGAIACYRGLTVKGGAAGVGAATTSSVVTAITTVIGFDTLFN
                     IVYIVFFP"
     gene            6614..7405
                     /locus_tag="VNW28_09365"
     CDS             6614..7405
                     /locus_tag="VNW28_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA10180.1"
                     /translation="MNDAPQHIVELRDVQLAFAEKKVLDGISLKVEPLDRLVIMGQSG
                     SGKSTILRLILGILQPNAGEIFFKQFEISRLQRRKLQHVRSRIGMVYQYSALLSSRNV
                     RDNIALPLEELTRKTRAEIDQIVDEKLELVGMQESKDQMPSELSGGMRKRVSLARALV
                     MDPELILFDEPSAGLDPVISSVIDELIISLTERSKVTSVIVTHEMGSAFRIATRMAML
                     YQGKVIADAEPEGLKKSDNPVVQQFLSGSTEGPILEGSKDAVATK"
     gene            7386..>7960
                     /locus_tag="VNW28_09370"
     CDS             7386..>7960
                     /locus_tag="VNW28_09370"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXA10181.1"
                     /translation="MQLRRNEITTGLLVLATVGILAFILILLGAPGLFRPLVVYKLYF
                     DNAAGIKLGAPVLLAGRKVGQVKLLNSPVSREEAAGALEAAGNLGTLNEPAGTLPHFE
                     VRIDVEVDRTALVYKNANVRLMTLGLLGDTAIDISGGNDHSGKSEDGQVFAGNRVPDF
                     SEAVAKMLGIIQPVATQATATMKELQNTVTNL"
BASE COUNT         1580 a         2465 c         2388 g         1527 t
ORIGIN      
        1 cttccaacgc gaacgcttct gcaaaagcag ttcgccagcg cagttgaaaa ggaagatgtg
       61 gatggcgcga tggcgcaagt tattcccatg cacctcgccc cggggcgcag ccccggtcac
      121 acgatcattt ttatcgacca cggcaaactg ctccttcggg tccgtgcccg cagccggcgg
      181 acctgctggc tgaacaagat ttgtcagcgc gatccattgc tcgagcgaca actcctccgc
      241 tctcgcctgg accgcggcgc caatctccgc cgccgccccc ggccaatcgg ccagctcttt
      301 ctgcagtaat ttcccaagtt gctttcggcg ctgggaaaat ccccggcgta ccagcctaac
      361 gaacaactcg tgatcgtgcg gcggcaactc gccgggcacc cgtggcagca gacgaacgag
      421 cgcggaatcg acctcggggc gcggaagaaa aacgctcgac gaaacggtgc gcaaataatc
      481 gacgcgatac tgcgattgca cgacgagggt gaggtttccg taatctttag agcgcggcaa
      541 cgccgagagc cgcgcggcca cttccttctg taacatcaac aacgccaata tgataggatt
      601 aggttgcgcg agaaagcgaa gtaatatctg agtcgagacg taatacggca gattgcctaa
      661 caacttcacc ggcccatgtt ggaaaagctg ccgggtgtcg aaatcgagcg catcgccatg
      721 aatcacctgc aggcgccggt tggcgaagtg agtgcggaga aaatccgcca accttccgtc
      781 cttctcaatg gccaggattt ctgcgccggt ggcgagcgcg ggctcggtca tcgcgcccag
      841 gccgggtccg acctcaagca cgaaatccgt ggcggaaagc tccgcccgct ccacgatcca
      901 gcgggcgaga ttctgatcgt gcagaaaatt ttgaccaagc gatttgaccg gcgcaacgcg
      961 gatttcgcgg aggatgtttc cgatttcgga aagtttcatc gctcgagaaa aaagaggggc
     1021 cgatttccga tggggcgaga cggtttgttc acagatcttt gtgaacaatc cgcctcgctg
     1081 gcaggcaatt attcacaagt tattcaaccg cgtatttgtg gtcgaaaata cgcgctgtta
     1141 cggccgattt cattaaatcc agcgtctatg gtttcgaact cggctcccgc agcgattttg
     1201 gtttttttgc gggtcgggca agggactttt tcttattctg agcaaacgcc cggcgcgcgt
     1261 cccgcaaggt tgcgccctcg acgaaataga ccgtggccgc gcggccgatc gcgaaggtgc
     1321 cggcaccggc gaccatcccg caaaccacat tcccccagcc cgggaaaaat ttgagtgccg
     1381 cccgcgtccc ttcgcgcaaa atcatcccga agccgacatt cgcgccgagc gcgccgacaa
     1441 attccgtcgc ggcgcgcatg ctccgctcgc gaccgctgat gtacatgatc cccgagacca
     1501 tgacgagttg cagagtcgtc aggatgggca gatccgcgag ggggatcggc tgcgccccga
     1561 ccgcggtgca gatggcggtg gtggatttga cgagcaactc cgcgatctcg gtctgcgcct
     1621 ggcggtcgcc gccgatccgc accatctcga cccgggcttc gttaggcaat tcgcgcgcca
     1681 gcgtgctgcg gaccgttccg cttaaggaat ggaccccgat caaactggcg cgaatcgcgg
     1741 gcgtgctcgt tgtctcgtcc ggaagagaac gcgattcggt gtcgttggcg cacagttcga
     1801 tcagcttcgc gccggccttg aagtcgatat tccaggcgag caatttttcc aatcgctcca
     1861 tttcgtgctg ccgcgtgacc ttcgcgtcgc gctcggccgg aagaaagaaa aacagatccg
     1921 cgggctgccc ccgcaactct tcacgcacga gcgcgagcgc ggcctcgtca gccccgcggg
     1981 catcgagcag ttggatcgaa ccgtgctgac cgatcatgat tttttgccag cgataaagac
     2041 cggtcagcgt gacgcgtgcc ggcgtctctg ctgcgccatc gctaaagatg cgggcgacga
     2101 aatcgagcag cggcatcggg tccgaaccgg tcaggacaaa acggggcgga cgttgctgga
     2161 gaaaaagctc tttcagcggc gtcagctcat gcagaaccgg ctttcgaatg gaatccggga
     2221 gccggccaag gaacgtctcc aggcgctcga cgatatggag caggacggaa ttgttcatcg
     2281 cgtgcaggtc atcgtgccca acatgaagcg aacgcggtcg atttgcgaat cggtgctgca
     2341 gacgccttcc tctgggaggc gcggggctgc ctgtcgccgc aagatgaagc cctgtttgct
     2401 ctcgcgcttg gcagaccgaa gcgtctccgg tggttgccgc agggtggaaa cgaacgccac
     2461 tccgtgctag agagacggcc cggcgggcgc actccgccta acgaatcatg ttttcacaac
     2521 tcggcgacaa attgcaggac atcttcaagg acctgcgcgg gcatggatcg atcagcgaaa
     2581 cgaacatcag cgatgcgatg cggcaggtgc gtctcgccct gctcgaggcc gatgtcgatt
     2641 accaggtggc gaaaaatttc gtcgcgcgga tcaaggaaaa ggcgctcggc gaagaagtgc
     2701 tccgcagcgt cacgccgggg caacaggtgg tcaagatttt tcacgatgag ctgaccgccc
     2761 tgctcggcgg cgacgccgct ccgctccagc tcgacaaacc cgcgcgcatt ttgatgatcg
     2821 gcctgaacgg cgccggcaag acgacttcgt cggccaagct ggcgctttac ttgaagaagc
     2881 aggggcgcgt gccgcttctc atcgcctgcg atttgcagcg gccggcggcg atcgagcaat
     2941 tggcaaccct aggcggacag atcagcgtac cggtttatgc cccctcccct gacgaaaaaa
     3001 acgtgaaacg tgccgccgcg gcgagcctcg aatgggccga gggacaaaac ggtaacgtgc
     3061 agattttcga caccgcgggc cgccaggaga tcgacacggc gctgatcgag gagatcaagc
     3121 agctcaagga atttctcaaa ccgcaggaag tgttgctcgt ggccgatgcc gcgacgggtc
     3181 agcaggcggt gagcgtcgcg acgcacttcc acgaagcgct cggcatcacc ggcatcgtcc
     3241 tgaccaaact ggacggcgat gcgcgcggcg gcgcggcgct ttccatgcgg gaggtgacac
     3301 aacgcccgat caagttcgcc ggtgtgggcg agaagctgga tcagttcgag ccgttcctac
     3361 ccgaccggct ggcgggacgg atcctcggga tgggcgacat cgtcgggctg gtggaaaaag
     3421 cggccgaagc gatcgacgag gaggaggcca cgcggatgga gcagaagctt cggaccgcgt
     3481 ctttcgattt caatgatttc ctcgcccagt tcaagatgat gcgaaagctc gggccgctcg
     3541 agagcattct cgggatgctg cccggcgtgg ggaacatgaa ggacctcaac atcgacgaga
     3601 accagctcaa gcggaccgag gcgatcgtcc tctcgatgac ggatcacgaa cgccgacgtc
     3661 ccgacattct gaacgcccgc cggcggcagc ggatcgcgcg cggcagcggc agcacggtga
     3721 cagaggtgaa cgctctgctc cagcgtttca accagatgcg caagatgatg aagaacgcgg
     3781 gcaagatgaa gcggatgatg gcgcagatgt cgaaaatgaa gagctgactt gcgatttgcc
     3841 cgatttaggc gattcgttcc ctccctcaga gccctcaatc cgccggaggc ggatcaacta
     3901 acaactttca gctctcaact ttcgccaatg gctgtttcaa tcagattacg cagagaaggc
     3961 gcgaaaaatc gcccttatta caaagtcgtg gtggccgaca gtcgcagccc gcgcgacggc
     4021 aaattcatcg agatcatcgg cacctacgac ccgaaaatgc agggtgaaaa cagcacgctg
     4081 aaagtcgacc gcgccgaaca ttggatcgcg cgcggcgcgc agccgtcgga cacggtgcgc
     4141 agcctgttga agaagaacaa ggcccgcgca gcggccgcac cagccgagcc ggttgccgcc
     4201 gaagcagcgg cggaatagtt gtagccgata atcgcctttt ccatccggga tcaccgagcc
     4261 cggctaccgc gcgccgattg gacaacgcgg gagcgcgtcg ctaagctcga atgtgcgaca
     4321 gtttctcgag tttatcattc ggcaactggt cgaattcccc gaggaagtga tgctctcgga
     4381 aattccgaat ggccgcatga ccgtcttccg gttgcaaatg cggcagaccg atgtcgggcg
     4441 aattatcggg cgcaacgggc aaacgatccg cgccattcgc gccttgctcg tgagcgcggc
     4501 ggcgcgtcac ggggagaagg cgacgctgga tatcgtggag taagttagtc atcccgagcg
     4561 aagccgaggg atcccggtgc gtgacccttc ggttttcttc gctaaatccc tggagcaaac
     4621 ttctagcttt ctccgcggga tccctcgact ccgctcggga tgactaccta tagatctcaa
     4681 cgttgacctt gccgtcctcc ccaactctac cgcgatacat tcccgaagta ttgaacggca
     4741 tcgcgaaatt cccttgcttg tcgatcgcga tcagaccgcc cgtcccgccc agccggccgg
     4801 ctttatcgag cgcggcctgg gccgctgcct gcacgggcat tcctttgtat tccatcatcg
     4861 ccgagatgtc gtgggccacg acggagcgaa taaaatactc gccatcacca gtgcacgaga
     4921 cggcgcatgt ccgattgtcg gcgtaggtcc ccgcaccgat gatcggtgaa tcgccgaccc
     4981 ggccgctttg cttgttcgtc gtcccgccgg tggaagtgcc ggccgcaaga ttccccgcct
     5041 tgtccagcgc gaccgcaccg acggtcccgt gccggtcctt gtcgctcatc ggcgcaggcg
     5101 aagttttcgc cttttccttc tggagcgact gccagcgctc attcgtgtaa aaatatttct
     5161 ggtcgacgaa tggcattccc attttcctgg cgaacgcttc cgcgccgtcg ccgacgagca
     5221 tgacgtgcgg cgatttttcc atcaccatcc gcgcgagact gatcgggttg cgaatgtgct
     5281 ggagatccgc gaccgcgccg gcgttgagcg ttttcccgtc catgatcgag gcgtcgagct
     5341 cgttcgttcc ttcgtgagtg aagacggcgc ctttgccggc gttgaaaagc gggttgtcct
     5401 cgagcacgcg gatcgcggct tcgacagcat cgaggctcga gccgccgcgt tcgagcaccg
     5461 cgtaaccggc cttgagggat tgttcgagcc ccgcgcgatg ggccgcttca ttctccggag
     5521 tcatctcgct gcgctcgatc gtgcccgcac cgccgtggat caccatgccg aatttcatcg
     5581 ggttattttt ctgttgcgct gcgctggtgc ccgcgagcac aaacccgccc agccagatcg
     5641 cgagatgaag tcgaagcatg aggtacgatt caggtgtgcg ccgaggggcc cacgtaaagc
     5701 attggtcgtt tcgaagccta atacttgccg ctcatctgga gtggggcaac ttcaccctgc
     5761 ttatgcccga caacgcttcc ccgacgcccg gcaccgcggg gacgattgtc cacgccccgg
     5821 gcgatcttct cgccggggtc ggagagaaga gccgctccct gctcctggaa gtcgggagca
     5881 tgttttggtt catcatcaat acgatcggcg aaacgctcga ccggatgcgc cgcgggaggg
     5941 tgcctttccg ggcggcgagt ttttttcgct acaccgaacg cgccggggtc ggctcagtgc
     6001 cgctggtcgc gatggtttct ttctttcttg gcctaacgat ggcgctcctg actggttacc
     6061 aactggagcg cttcgggacg gagcgactcg tgcccggact ggtggcgatc tccttcaccc
     6121 gcgagctcgg gcctctgctc accggcatca tgctcgcggc gcggatcggc gcctcctaca
     6181 ccgcggagct cgggacgatg caggtctcgg aggaagtcga ggcgatcgag gcgatgggca
     6241 tcgggccgct gcggtttttg gtcgcgccgc gtctcctcgc gctcttcgcc cttatgcctt
     6301 gcttgagcgt catctccagc gtcgctgcga tctttgcctc ggcgctgatc tcgcgcgcct
     6361 acttcagcat cgcgtttgtt tatttccagg atctcgtcct gcaaagcctg ctcatccgcg
     6421 acattgtcac cggcatcctg aagagcttca tgttcggact gctcatcggc gcgatcgcct
     6481 gctaccgcgg gctgacggtg aaaggcggcg cggctggcgt cggcgcggca accacctcga
     6541 gtgtcgtcac cgcgatcacc accgtgatcg gtttcgacac tctcttcaac atcgtctaca
     6601 tcgtcttttt cccgtgaacg acgcgcccca acacatcgtc gaactgcgcg atgtgcagct
     6661 cgcgtttgcc gaaaagaagg tgctcgacgg catttccttg aaagtcgagc cgctcgatcg
     6721 gctcgtcatc atgggccaga gcgggagcgg caagagcacc atcctgcgcc tgatcctggg
     6781 catcctgcag ccgaacgcgg gcgagatttt cttcaagcag tttgagattt cgcgcctgca
     6841 gcgtcggaaa ctgcaacacg tgcgcagtcg catcgggatg gtttatcaat attccgccct
     6901 cctcagctca cggaacgtgc gcgacaatat cgctctccca ctcgaggagc tgacgcgaaa
     6961 gacgcgcgcc gagatcgacc agatcgtgga tgaaaagctc gaactcgtcg ggatgcagga
     7021 atccaaggac caaatgcctt ccgagctgag tggcgggatg cgcaaacgcg tcagcctcgc
     7081 gcgcgcgctc gtcatggatc ccgagctgat cctgtttgac gaaccctccg ccgggctcga
     7141 cccggtcatc agctcggtga tcgacgaatt aatcatcagc ttgaccgagc gctccaaggt
     7201 gacctcggtt atcgtcaccc atgaaatggg cagcgccttt cgcattgcca cccgcatggc
     7261 catgctctac caaggcaaag tcatcgcgga cgccgagccc gaggggctca agaaatcgga
     7321 caatccggtc gtgcagcaat tcctgagcgg cagcaccgag ggtcccatcc tggaaggaag
     7381 caaagatgca gttgcgacga aatgaaatca cgacaggcct gctcgtgctc gccacggtcg
     7441 ggatccttgc cttcatcctc atcctgctcg gcgcgccggg attgttccgt ccgctggttg
     7501 tttacaagct ttacttcgac aacgccgccg ggatcaagct cggcgctccc gttctcctcg
     7561 ccggccgcaa agttggccag gtcaagttgc tcaattcccc ggtctcgcgg gaagaagccg
     7621 ctggcgccct cgaagccgcc ggcaacctcg gcactctaaa tgaaccggcc ggcaccctcc
     7681 cccatttcga agtgcggatc gacgtcgagg tcgatcgcac cgccctcgtt tacaagaacg
     7741 ccaacgttcg gctcatgacc ctgggattgt taggcgatac ggcgatcgac atctccggcg
     7801 gcaacgatca ctccggcaaa tccgaagacg ggcaggtctt tgccggcaac cgcgtgccag
     7861 atttctcgga agcggtcgcg aaaatgctcg ggatcattca accggtcgcg acccaggcga
     7921 ccgcgacgat gaaggagttg caaaacaccg tcaccaacct
//
LOCUS       DATMNS010000267         2961 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2041173, whole genome shotgun sequence.
ACCESSION   DATMNS010000267 DATMNS010000000
VERSION     DATMNS010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2041173"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..1041)
                     /gene="rfbB"
                     /locus_tag="VNW28_09375"
     CDS             complement(49..1041)
                     /gene="rfbB"
                     /locus_tag="VNW28_09375"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HXA10182.1"
                     /translation="MRILVTGGCGFIGSNFIRYILQHYKPAYVTNVDALTYAGNLANL
                     DGVVEEHGARYEFFQADIANPDQMDTLLHEEQIYAIINFAAESHVDRSINSPQNFIHT
                     NVVGTSTLLDAARRHGVQRFLQVSTDEVYGSLGATGRFSEQSPLAPSSPYSASKAGAD
                     LLALACHHTFGQDVIVTRCSNNYGPYQFPEKLIPLMIIKALSDQPLPVYGDGLNVRDW
                     IHVEDHCAALVAALFNGQPGRIYNLGGEGEMVNLDVVRLILDRLGKPHSLISFVTDRL
                     GHDRRYAIDSSLAKGELDWHPQHNAREGIAATVDWYLNHRDWWQALLERTERFD"
     gene            1007..2905
                     /locus_tag="VNW28_09380"
     CDS             1007..2905
                     /locus_tag="VNW28_09380"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase activity-associated protein Agl23"
                     /protein_id="HXA10183.1"
                     /translation="MNPQPPVTKMRIGALPNSGEQPARGEIDAAAPPPLGERLARIDW
                     TFWAIIALGALLRFLLLSIKPPHFDEGINGWFVDQMRRNGFYRYDPTNYHGPLHFYIL
                     FLAQSLFGRHIWALRLPVVLASIGSIFLTLKFEPLVGRTISRFAALAMAISPAFIFYG
                     RYSIHEVWLLLFSMLFVLGLLGLWRFGTRGYLWCVGIGVAGMILTKETYFIHIGCALI
                     AGVVLWISHRITPLPELKRARQQWDLVDLAMVAGTGIFFIVFFYSGTFLNWPGLKGLY
                     QTFGTWFRTGSNGNGHEKPWPYWLELTVRYEWPVFIGLTLCLACQFFKNFVIRYLAIY
                     GVGLFIAYSIIHYKTPWIIISVVWPFLFVFAAGATARKIPRPAFYVVGFALVGFGLGA
                     VASFLVQAATMPASCTWPVYLREAVNITIAASSTSPVCGEIGERLFGSAVFGALLGGG
                     LGFLIGKSSKISEGVMRAVQRGAVGLALAISLALAIFLNYFRCSTDSEPYVYVQTYND
                     IYRLMNPLMRLVRSNPLNYRMVGHFIRTSTYPFPWLLGDFANVGYYENHNSPGKFDAD
                     FLVVQSDRIAEVEKKLHESYFTEPMTIRPYQDTSKLYFNAKTFGKLFRGRKPDFVGQA
                     APTPATPR"
BASE COUNT          520 a          857 c          928 g          656 t
ORIGIN      
        1 atccgggcga ggcggatcgt cccttgagaa ggcgcgcgcg gttgggcgct agtcgaaacg
       61 ctccgtccgc tcgagcagcg cctgccacca gtcgcgatgg ttcagatacc agtcgacggt
      121 cgcggcgatg ccttcgcgtg cgttgtgctg cgggtgccaa tcgagttcgc ctttcgccag
      181 tgacgaatcg atcgcataac gtcgatcgtg gccaagccgg tcggtgacga acgaaatcag
      241 gctgtgcggt ttgccgagcc gatcgagaat cagcctaacg acatcgaggt tcaccatctc
      301 gccctcgcct ccgaggttgt aaatgcggcc cggctggccg ttaaaaagcg ccgcgacgag
      361 ggcggcgcaa tggtcctcga cgtggatcca gtcgcgcacg ttgagcccgt cgccgtaaac
      421 cggcagcggc tgatcgctca gcgccttgat gatcatgagc gggatcagtt tttcggggaa
      481 ctggtagggg ccgtaattgt tggagcaacg cgtgacgatg acatcctgcc caaaagtgtg
      541 gtggcaggcg agcgcgagga gatcggcgcc ggccttgctc gcggagtagg gggaactggg
      601 cgcgagcggc gattgctcgc tgaaccggcc ggttgcgcct aacgaaccgt aaacttcgtc
      661 ggtcgaaacc tggaggaagc gctgcacccc gtggcggcgg gcggcgtcga gcagggtgct
      721 cgtcccgacg acgttggtat ggatgaaatt ttgcggggaa ttgatgctcc ggtcgacgtg
      781 cgattcggcc gcgaaattga tgatcgcgta aatctgctcc tcgtgcagga gggtgtccat
      841 ctggtcgggg ttggcgatgt cggcctggaa aaattcatag cgcgcgccgt gctcctcgac
      901 gacgccgtcg agattggcga ggttgccggc gtaggtgagg gcgtcgacat ttgtcacgta
      961 cgccggcttg taatgttgca gaatgtaacg aataaagtta ctcccgatga acccacagcc
     1021 gcctgtcacc aagatgcgca tcggagcgct acctaacagc ggagagcagc ccgcgcgagg
     1081 agaaattgac gcagccgcgc cgccgccgct cggagagcgg ctcgcccgga tcgattggac
     1141 gttctgggcg atcatcgcct tgggcgcgct cctccggttc ctcctcctga gcataaagcc
     1201 gccgcacttc gacgagggga tcaacggctg gttcgtcgat cagatgcgga ggaacggctt
     1261 ttaccgctac gatccgacga attatcacgg gccgctccat ttctacattc tcttcctcgc
     1321 gcaatcgctt ttcggccgcc acatctgggc gctgcggctg ccggtggtgc tggcgagcat
     1381 cgggtcgatt ttcctgacgc tcaaattcga gccgctggtc gggcggacga tcagccggtt
     1441 cgcggccttg gcgatggcga tctcgccggc tttcatcttt tacgggcgct attcgatcca
     1501 cgaagtgtgg ctgctgcttt tctcgatgct cttcgtcctc gggctgctcg gactctggcg
     1561 ctttggcacc cgcggctacc tttggtgcgt agggattgga gtggccggga tgatcctgac
     1621 gaaggagact tacttcatcc acatcggctg cgcgctcatc gcgggagtgg tgctctggat
     1681 ttcgcaccgg ataacgccgt tgcccgagct gaagcgcgcg cggcaacagt gggatctcgt
     1741 cgatctcgcg atggtcgccg ggaccggcat ttttttcatc gtcttctttt actcgggcac
     1801 gtttctcaac tggccgggcc tcaaagggct ctatcagaca tttggcacct ggtttcgcac
     1861 cggttcgaat ggcaacggcc atgaaaagcc gtggccctac tggctcgaac tgacggtgcg
     1921 ctatgagtgg ccggtcttca tcggactcac gctttgcctc gcctgccagt tctttaagaa
     1981 ctttgtcatc cgctacctcg cgatctatgg cgtcgggctc ttcattgcct acagcatcat
     2041 ccactacaag acgccgtgga tcatcatcag cgtcgtctgg ccattcctgt ttgttttcgc
     2101 ggcgggggcg accgcgagga agattccgcg cccggctttt tacgtggtcg gttttgcact
     2161 cgtcgggttt ggcttgggag cggtcgccag tttcctggtc caggccgcga cgatgcccgc
     2221 aagctgtacc tggccggtct atttgcgcga agccgtcaac atcacgatcg ccgcgtccag
     2281 cacgagtccg gtctgcgggg aaatcggcga gcggttattc ggctcggctg ttttcggcgc
     2341 cctgctgggc ggagggcttg gtttcctcat cggaaagtct tcgaaaataa gcgaaggagt
     2401 gatgcgagcg gtgcaacgcg gcgcggtcgg tctggcgctg gcgatctcgc tcgcgctcgc
     2461 catttttttg aattacttcc gctgcagcac cgacagcgag ccgtatgtct atgttcagac
     2521 ctacaatgat atctaccggc tgatgaaccc gttgatgcgg ctggtgcgga gtaatccgct
     2581 caactatcgc atggtcggtc atttcatccg gacaagcact tatccgttcc cgtggctact
     2641 gggcgatttc gccaacgtcg gctattatga gaatcataac tcgccgggca aattcgatgc
     2701 ggatttcctt gtcgtgcaga gtgatcgcat tgcggaagtg gagaagaagc tgcatgagag
     2761 ctacttcact gagccgatga ctattcggcc ttaccaggac acctcgaagc tctatttcaa
     2821 cgcgaagacc ttcgggaaac ttttccgggg caggaagccg gacttcgtcg gccaggccgc
     2881 accgacgccg gcgacacctc gatgaagttc tttgcggcgg gattgactct cgtcaacatc
     2941 gcaaccatcg ccgggctgct c
//
LOCUS       DATMNS010000268         5062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3469803, whole genome shotgun sequence.
ACCESSION   DATMNS010000268 DATMNS010000000
VERSION     DATMNS010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5062
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3469803"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VNW28_09385"
     CDS             complement(<1..339)
                     /locus_tag="VNW28_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HXA10184.1"
                     /translation="MIPHDKLFTPGPVEVSEKTLAAFSRPMIGHRGDDFKNLYRAIHP
                     RLQELFGTKQPVFLSTSSAWGVMEAAIRNLVRQRVLCCMCGAFSDKWLEVARRCGKEA
                     EPLQVEWGQAI"
     gene            495..1208
                     /locus_tag="VNW28_09390"
     CDS             495..1208
                     /locus_tag="VNW28_09390"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA10185.1"
                     /translation="MATVSQMTADPALETHLFWVQYKTTIIAVVAVLVLGALGYAGFL
                     LYTTRRAADATALLSAAKSAQDYQQLIDRYPGSEPAASAYLLLAAQQRATRDYTGANT
                     TLHKFIDQYPKHELITTAWMGVAANLESLGKNDEALSTYQRLVTEYPQSFNAPLAMLA
                     EVPFLKAKSRFDEARRVCETLLTQYRDSIVANDAMRELTSLPQPTASAKSPAQVTLQA
                     EPSIPMARPEETAAPAATP"
     gene            complement(1298..1693)
                     /gene="rpsI"
                     /locus_tag="VNW28_09395"
     CDS             complement(1298..1693)
                     /gene="rpsI"
                     /locus_tag="VNW28_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXA10186.1"
                     /translation="MAETQEFIATGRRKTAVARIRMTPGNGKIDINGKNFEDYFPTVP
                     LQNTVLQPLQVVKLTNAYDISVNTTGGGATGQAGAARLAISRALLQVDANLRGTLKAE
                     GLLRRDPRMKERKKSGQPGARKRFQFSKR"
     gene            complement(1706..2137)
                     /gene="rplM"
                     /locus_tag="VNW28_09400"
     CDS             complement(1706..2137)
                     /gene="rplM"
                     /locus_tag="VNW28_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXA10187.1"
                     /translation="MKTFSAKADQVDRKWWIIDARDQVLGRVAVKAAKLLRGKEKAIV
                     TPHVDTGDFVVVINADKVRLTGKKEEQKSFMSFSGFVGGHKTETARSRRARHPELLVE
                     HAVRGMIPHNKLGRAVYRKLKVYSGDEHPHVAQQPQTVTLD"
     gene            2241..2909
                     /locus_tag="VNW28_09405"
     CDS             2241..2909
                     /locus_tag="VNW28_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXA10188.1"
                     /translation="MRKVLILAILPAIFCVCTATAQSNGSPGPATAGAEVIREMNLAR
                     QHPERYALQLEELRGHFRGDVLILPGRTMLRTREGAGAVDDAIRFLQRARPLAPLIIS
                     PGISLAAAEHVADQAAGAFGHGGSDHSDPGERMNRHGTWSALWGENIAYGKATARDAV
                     VALIIDDGLRSRKHRKNIFNPAFNYAGAALGPHACYGTVCSIDFAGGYVELANTSRSL
                     LAHN"
     gene            3034..4602
                     /gene="proS"
                     /locus_tag="VNW28_09410"
     CDS             3034..4602
                     /gene="proS"
                     /locus_tag="VNW28_09410"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXA10189.1"
                     /translation="MTQPDLPKSAISPRRAEDFPEWYQQVIRAGDMAEPSNVHGCMVI
                     KPWGYGIWENMQRALDAMFKATGHKNAYFPLFIPLSYMQKEAEHVEGFAKECAVVTHH
                     RLELNADGKMVPASPLTEPLVIRPTSETIIGASYAKWVQSYRDLPILINQWANVVRWE
                     MRPRLFLRTTEFLWQEGHTVHETEAEARAETRQMLEVYATFARDYLAVPVYTGEKSES
                     ERFPGAVQTLCLEAMVQDRKAIQAGTSHFLGQNFSRASGIRFQTRDGKQEFGWTTSWG
                     VSTRLVGTLIMMHADDDGLVLPPRIAPTHIVILPVTPKPETRDAVLAAAHELATQLRA
                     LTWHGAPLEVHIDQRDLGGGVKNWEWIKKGVPLRVELGPRDLESGNVAVTRRDGLPVA
                     GTADPGKPAPKSFLPSAIFVRQATELLDAIQQNLFARALAFRDAHTRALETKEEFYKF
                     FTPQNPAKPEIHGGFARAHWDGSRAVEEQIKNDLKVTIRCIPFDDPQNPPESGQCVIT
                     GEPSARRVLWAKSY"
     gene            complement(4877..>5062)
                     /locus_tag="VNW28_09415"
     CDS             complement(4877..>5062)
                     /locus_tag="VNW28_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10190.1"
                     /translation="RRLSKANSRCQAGRGEESEEQIAIHFFLIITGSRPAETAEINPF
                     TGGPVKHSGIALRCANK"
BASE COUNT          974 a         1594 c         1528 g          966 t
ORIGIN      
        1 gatcgcctgc ccccactcga cctgcagcgg ctccgcttcc ttgccgcagc gccgcgcgac
       61 ctcgagccac ttgtcggaaa acgcaccgca catgcagcag aggacgcgtt gcctgaccag
      121 attgcgaatc gccgcttcca tcacgcccca ggccgaggag gtggaaagaa aaaccggctg
      181 tttggttccg aatagttcct ggaggcgcgg atggatcgcg cggtagaggt ttttgaaatc
      241 gtcgccgcga tgaccgatca tcggccggga aaaagccgcg agcgtcttct ccgagacctc
      301 gaccggaccg ggcgtgaaaa gtttgtcgtg tggaatcacg gctgaaacgt ccaacgtcca
      361 acccgccttc gcaacgcttc ggcgcggcag gcgtccaacg tccaacgttc aactcggcgg
      421 gcctccgggg ccgcgccggg aaggggcttg cgcggaaaac cttcgcgtgg catagacaca
      481 actcccgatt ttccatggcc actgtatcgc aaatgaccgc cgatcccgcg ctcgagaccc
      541 atcttttctg ggttcaatac aagacgacca tcatcgcagt cgtcgctgtc ctggtgctcg
      601 gcgcactcgg ttatgctggt ttcctgctct acaccacgcg ccgcgccgcc gacgcgaccg
      661 cccttttgag cgccgcaaaa tcagcacagg attatcagca gttgatcgat cgttatcccg
      721 gcagcgaacc ggccgcgagc gcctacttgc tcctcgccgc gcaacaacgg gcgaccagag
      781 actacaccgg cgcgaacacg accctgcaca aattcatcga ccagtatcca aaacacgaac
      841 tgatcacgac cgcgtggatg ggtgtggcgg caaatctcga gtcgttaggc aagaacgacg
      901 aggcactcag cacctatcag cggctcgtga ccgaataccc gcagagcttt aacgcgccgc
      961 tcgcgatgct ggcggaggtg cccttcctga aagcgaagag ccgcttcgac gaagcgcgcc
     1021 gggtttgcga aacactgctg acccagtatc gcgacagtat cgtggccaac gatgcgatgc
     1081 gggaattgac ctctttgccg cagcccacgg cgagcgccaa gtcgcccgcc caggtaacgc
     1141 tccaggcaga accctccatt ccgatggccc gaccggaaga gactgccgcg ccggccgcga
     1201 cgccgtagcc gcttcgctct gcgaagcgcg gcctgacgcg ctcatcgtcg gaccagcgaa
     1261 cgagctttcc cgcgcagagc gaggcgtcgg ccagcgctta ccgcttcgag aactggaacc
     1321 gcttgcgcgc gcccggttga ccggacttct tccgttcctt catccgcgga tcgcgcctca
     1381 gcaacccttc ggctttgagc gtgccgcgaa gattcgcgtc gacctgcaac aaggcgcgcg
     1441 aaatcgcaag gcgcgcggcc ccggcctgac cggtggcgcc gccgccggtg gtgttgaccg
     1501 agatgtcgta cgcgttggtg agtttcacca cctggagtgg ttgcagcacc gtgttttgca
     1561 gcggcacggt cgggaaataa tcctcgaagt tcttgccgtt gatgtcgatt ttgccgttac
     1621 ccggcgtcat gcggatgcgc gcgacggcgg ttttgcggcg accggtggcg ataaattcct
     1681 gcgtctcagc catgaaagta gttcgttagt ccaacgtaac cgtctgcggt tgctgcgcga
     1741 cgtggggatg ttcgtcgccg ctgtagactt ttagtttccg ataaaccgcc cggccaagtt
     1801 tgttgtgtgg aatcatcccg cggacggcgt gttccaccag cagctcggga tgccgcgcgc
     1861 ggcgggagcg cgcggtctcg gttttgtggc caccgacaaa acccgagaag ctcatgaagg
     1921 atttttgttc ctccttcttg cccgtcagtc gcaccttgtc ggcgttgatc acgaccacga
     1981 aatcgccggt atcgacgtgg ggcgtaacga tcgccttttc tttgccacgg agcaacttgg
     2041 cggctttgac cgcgacgcgg ccgagcacct gatcacgggc atcgatgatc caccatttgc
     2101 ggtcgacctg atctgctttt gcggaaaacg ttttcatcgg ggagttctcg ggaagggcgg
     2161 ggaaattagt ggcggcgaca gcaggtgtca aatcccgccc cggtcgaggc agtggcaccg
     2221 cggatgcttt ctcccagagg atgcgaaagg tgctcattct ggccattctg ccagcgattt
     2281 tctgcgtctg caccgcgacg gcgcaatcaa atgggtcgcc cggtcccgcg acggccggcg
     2341 ccgaggtcat ccgcgagatg aacctcgcgc gtcaacatcc ggaacgctac gctcttcaac
     2401 tcgaggaatt gcgcggtcat ttccgtggag acgttctcat tcttccgggc cggacgatgt
     2461 tgcgcacccg cgaaggcgca ggcgcagtgg acgacgcgat ccgattcttg cagcgcgcgc
     2521 gcccgctcgc gccgttgatt atttcgcccg gcatttccct ggctgcggca gagcacgtcg
     2581 ccgatcaggc tgcgggcgcc ttcggtcatg gcggatctga tcacagtgac ccgggcgaaa
     2641 ggatgaatcg ccatggaacc tggagcgcac tctgggggga gaacattgcc tatggcaaag
     2701 caacggcgcg agacgcggtc gtcgcattga tcattgacga cggcctgcgc agccggaaac
     2761 atcgcaagaa cattttcaat cccgccttta attacgccgg cgcggcactg ggaccgcatg
     2821 cttgctacgg cacggtttgc agtatcgatt tcgccggtgg ctacgtcgag ttggccaaca
     2881 cttcgcgttc gctcctggcg cacaactaaa gtcgggtatt gatcccattc ggcctctcgg
     2941 tcggatgaga cccatgggac gaataggacc tgtggctttg ccaaaacccc ggctggtcaa
     3001 ccgtcccgcg tcggtttaga tatcgcgttc gccatgaccc agcccgacct tcccaaatcc
     3061 gccatcagcc cgcgccgcgc ggaggatttt cccgagtggt accagcaggt cattcgcgcc
     3121 ggcgacatgg cggagccgag caatgtgcac ggctgcatgg tgatcaagcc gtggggctac
     3181 ggcatctggg aaaacatgca gcgcgcgctc gacgcgatgt tcaaggcgac cgggcacaag
     3241 aacgcctatt tcccactctt tattccgctc agctacatgc agaaggaagc ggaacacgtc
     3301 gaaggtttcg ccaaggaatg cgcggtcgtc acccatcatc ggctagagtt gaatgccgac
     3361 ggaaaaatgg tgccggcgag cccgttgacc gagccgctgg tcatccggcc gacctccgag
     3421 acgatcatcg gcgcgagcta cgcgaagtgg gtgcaatcct accgcgacct gccgatcctg
     3481 atcaaccagt gggccaatgt cgtccgctgg gaaatgcgtc ctcgtttgtt tttgcggacc
     3541 accgaatttc tctggcagga aggtcacacc gtgcacgaaa cggaagccga ggcgcgagcc
     3601 gagacgaggc agatgctcga ggtttacgca accttcgcgc gcgattacct ggcggttccg
     3661 gtttacaccg gcgagaaatc ggagagcgag cgtttccccg gcgcggtgca aacgctctgc
     3721 ctcgaagcga tggtgcagga tcgcaaggcg atccaggctg gcacctcgca ttttctcggg
     3781 cagaattttt cccgcgcaag cgggattcga ttccaaacac gcgacggaaa acaggagttc
     3841 ggctggacga cgagctgggg tgtgagcacc cgccttgtcg gcacgctcat catgatgcac
     3901 gcggatgacg acggcctcgt cctgccgccg cgcattgcac cgacccatat cgtcattctg
     3961 ccggtcacgc cgaaaccgga aacgcgtgac gcggtgctgg ccgcggcgca cgaactggcg
     4021 acgcaattgc gcgctctcac ctggcacggc gcgccgctcg aggtgcatat cgatcagcgg
     4081 gatcttggcg gcggtgtgaa aaattgggaa tggatcaaga aaggcgtgcc gttgcgcgtc
     4141 gaactcggcc cgcgcgacct ggaaagcgga aacgttgccg tcacgcggcg cgacggcctt
     4201 cctgtggcgg ggaccgccga ccccggcaaa ccggcgccga agtcattcct gccgagcgct
     4261 attttcgttc gccaggcaac tgagctgctc gatgcgatcc aacaaaatct ttttgcgcgc
     4321 gcgctcgcat ttcgtgatgc gcacactcgt gcgctcgaga ccaaggaaga gttctacaag
     4381 ttcttcactc cccagaaccc agccaagccg gagatccacg gcggctttgc ccgcgcccac
     4441 tgggacggca gccgcgcggt cgaggagcag atcaagaacg acctcaaggt cacgattcgc
     4501 tgtatcccgt tcgacgatcc ccaaaacccg ccggagagcg ggcaatgcgt catcaccggc
     4561 gagccgagcg cgcgacgcgt tctctgggca aaatcctatt aacggcgtcc cccggcgcgt
     4621 ttggaggtgt ctcagtttcc ccttgttcgt taggcgccct tcatcgatcg gaagctgaag
     4681 ccagcgatca gcgtcgtctg gaaatccgct tccggggcga cgttggtcag ttgaataagc
     4741 cgcgtctccc accgccaaaa ggcgctcgtg atttcgaatg ttattgaccg gcgatatggc
     4801 cgcgacgcgc aaccttcacg ccgctgcctg gcccgggcga ttcgccaaaa gttacaggga
     4861 cgttactttt cgttcgctac ttattcgcgc accggagggc gatgcccgag tgtttcacag
     4921 gaccaccggt aaagggattg atttccgccg tttcggcggg cctcgatcct gttatgatga
     4981 gaaaaaaatg aatcgcgatt tgctcctccg actcctcgcc gcgtccagcc tgacaccggc
     5041 tgtttgcctt ggacaggcga cg
//
LOCUS       DATMNS010000269         3969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4147995, whole genome shotgun sequence.
ACCESSION   DATMNS010000269 DATMNS010000000
VERSION     DATMNS010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4147995"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1826)
                     /locus_tag="VNW28_09420"
     CDS             complement(120..1826)
                     /locus_tag="VNW28_09420"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HXA10191.1"
                     /translation="MKNILFPLAITLGLVLPLRAGSETPSPADTIFVNGNVYTVNDAR
                     PHAAAIAIRGERILFVGSNEEAKKYSGPSTRTIDLAGKTVVPGLTDSHYHILGVGERL
                     TRLNLEDATSREIFLNEVKQAAQKSEPGRWLTGRGWIETFWKPPVFPTREDVDRVAPA
                     IPVFLVRADGHSAVANGAALKAAGIDRTTADPFGGQILRDKKTGEPTGMLLDNAMELV
                     EKKIPPSSPEDNEKAIFLGALRTVQLGWCKIQNAGSYLPQLELMEKLYGQGKLKLRIY
                     NAVYGPGEAADKILQDGPILDAYDHHFTTRTIKIVFDGSLGSRSAALLEPYSDADTSG
                     FLTQKEADLRPMLRAALRRGIQVETHAIGDRANRIILDLYEEAFQAVPPNERKVKEPR
                     WRVEHAQILSPSDIPRFAKLGVIASMQPSHAISDLFFAPSRLGEKRLAGAYAWQSLLK
                     SGAIITAGSDAPVQQGNPMIEFYAAVARKSQNGFSGPGWHPEQAVSRQQALEMLTRWP
                     AYAAFQENEKGSIAPGKLADLSILSADIMQIPEPDILRTHCLMTVIGGEIAYDAGELA
                     HP"
     gene            complement(1823..2584)
                     /locus_tag="VNW28_09425"
     CDS             complement(1823..2584)
                     /locus_tag="VNW28_09425"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXA10192.1"
                     /translation="MPNILVEEKDSRTTILTLNRPERRNALTIELMTELATAVESAAA
                     DEKRRILILRGAGKAFCAGLDLQETTEMEKAHRSAEMVARTLRTISETRLITIAQIHG
                     AAVAGGAGLMSACDFVLAVAHTRIGYPEAKRGLVAGLVMTFLRRQLRERDLRELLFTG
                     DLIGAERAREMGLVNRVVTPNELENETQKLTTAILQGAPGAISNTKRLIEELWSSSVG
                     EDIDLALRHHMEARESAEAKEGIHAFLEKRPPNWT"
     gene            2728..3594
                     /locus_tag="VNW28_09430"
     CDS             2728..3594
                     /locus_tag="VNW28_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HXA10193.1"
                     /translation="MPDQNVKLIECPRDAWQGLPGHIPTERKVRYLRALIDAGFRHID
                     AVSFVSPKAVPQMADSEAVLAQLAPPDDVEIIGIVVNEKGAERAIATGAVRTLGYPCS
                     ISETFLRRNQNQSPEENRATLTRIHEQAEASGLKMVVYISMAFGNPYQDLWSIERLVE
                     AVRGIAALEVGSISLADTVGMAKEKMIGEVVAAVVAGIDDPGLEIGVHLHSTRPEAAA
                     KVLAAYDAGCRRFDAALGGLGGCPFAQDELVGNIPTEEVIRALSQRGIQLPLTPEKAT
                     SLNERISQQFSS"
BASE COUNT          748 a         1181 c         1267 g          773 t
ORIGIN      
        1 acgccatagg ccagggccac gcgatagacc cgccgctgtt ttagttcgcg aagaaaaccg
       61 cgcatgggct gatgacgtta cacgttccga atgagggctg cgagatggag cgccggggtt
      121 tacgggtgag ctaactcgcc cgcgtcgtag gcgatctcac cgccaatcac cgtcatcaga
      181 caatgggtgc gcagaatatc gggctcgggg atttgcatga tgtcggccga gagaatcgac
      241 aggtcggcca gctttcccgg cgcgatcgag cccttctcat tttcctgaaa tgcggcgtag
      301 gccggccagc gggtcagcat ctcgagcgct tgttgccgcg aaacggcctg ctctggatgc
      361 cagcccgggc cactgaatcc gttttgactt ttccgcgcga ccgcggcgta gaattcgatc
      421 atcgggttgc cctgctgcac cggcgcgtca gagcccgccg tgataatcgc gccgctcttg
      481 agcaaactct gccaggcgta ggcgccggcg agccgcttct cgccgagccg actgggcgcg
      541 aagaaaagat cgctgatcgc atgcgacggc tgcatcgagg cgatcacgcc taacttcgcg
      601 aagcgcggga tatcggacgg gctgaggatc tgcgcgtgtt cgacgcgcca gcgcggttct
      661 ttcactttgc gctcgttagg cgggacggct tggaacgcct cctcgtaaag gtcgaggatg
      721 atgcggttgg cccgatcgcc gatggcatgc gtctcgacct ggatgccgcg gcgcagcgcg
      781 gcgcgcaaca tcgggcgcag gtccgcttcc ttctgggtga gaaaccccga ggtgtcggca
      841 tcggagtatg gctccaggag tgcggcgctg cgcgaaccga gcgagccgtc gaacacgatc
      901 ttgatcgtgc gcgttgtgaa atgatgatcg taggcgtcga gaatcggacc gtcctgcagg
      961 attttgtcgg ccgcttcacc cgggccatac actgcattgt agatgcgcag cttgagcttg
     1021 ccttgcccgt aaagcttctc catcagctcg agttgcggga ggtagctacc ggcattttgg
     1081 attttgcacc agccgagttg gacggtgcgg agcgcgccca ggaaaatcgc tttctcgtta
     1141 tcctccggcg acgacggcgg gatttttttc tcgaccaact ccatcgcgtt atcgagcaac
     1201 atgccggtcg gttcaccggt ctttttgtcg cgcaggattt gtccgccgaa tggatcagcc
     1261 gtcgtgcgat cgatccccgc cgctttcagc gccgcgccat tcgcgaccgc gctgtggccg
     1321 tcggcgcgca cgaggaaaac cggaatcgcg ggcgcgaccc ggtcgacatc ttcccgggtc
     1381 ggaaagaccg gcggtttcca aaacgtctcg atccaaccgc ggccggtgag ccagcggcca
     1441 ggttcacttt tctgagccgc ttgcttcacc tcgttaagaa agatttcgcg gctcgtcgcg
     1501 tcctcgagat tcagacgggt taaacgctcg cccacgccca ggatgtggta gtgcgaatcc
     1561 gttaggccgg gcacgactgt ttttccggcg agatcgatcg tgcgggtgga cggaccggaa
     1621 tatttcttcg cttcttcatt cgaacccaca aaaagaatcc gctcgcccct gatggcgatc
     1681 gcggcggcgt gcggccgggc gtcgttcacc gtgtaaacgt tgccgtttac aaaaatcgtg
     1741 tcggccggcg acggagtttc ggatccggca cgaagaggaa ggacgaggcc gagagtgatg
     1801 gcgagcggaa agagaatgtt cttcacgtcc agttcggtgg gcgtttctcg aggaaggcgt
     1861 ggatgccttc tttggcttcg gccgactcgc gcgcttccat gtgatgacgc agcgccagat
     1921 cgatatcctc accgaccgaa gaagaccaga gttcctcgat gaggcgtttg gtattggaga
     1981 tcgccccggg agcgccctgc aaaatggcgg tcgtcagttt ttgcgtttcg ttctccaatt
     2041 cgttaggggt gacgacccgg ttgaccaggc ccatctcgcg ggcgcgctcg gcgccgatca
     2101 aatcgcccgt gaaaagcaat tcgcgcagat cgcgctcgcg cagttgccgc cggagaaagg
     2161 tcatcaccag gccggcgacc agaccgcgtt tcgcctcggg atagccaatc ctggtgtgcg
     2221 ccacggcgag gacaaagtcg caggccgaca tcagaccggc gccgcccgcg accgcggccc
     2281 cgtggatctg cgcgatcgta atcaagcgcg tctcgctgat cgtgcgcaag gttctcgcca
     2341 ccatctcggc ggagcgatgt gctttttcca tctcggtggt ttcctgtaga tccagtccgg
     2401 cgcagaaggc cttgcccgcg ccgcgcagga tgaggatgcg ccgcttttcg tcggccgcgg
     2461 cgctttcgac ggcggtggcc agttcggtca tcaactcgat cgtgagagcg ttgcgccgct
     2521 ccggacggtt gagggtgaga atcgtggtgc gggaatcttt ttcttcaacg aggatatttg
     2581 gcataacgaa tgggttcgcg agtttaagcg ggctgcgctg agctcgcgag acccaaaacc
     2641 gatcgccgga aaaacgcaat ggcaaaacag ggcttgcagc gagcgcgacc gcaatgacgc
     2701 tctctcgacc agagcgcact tttcgtgatg ccagaccaga acgtgaaact gatcgagtgc
     2761 ccgcgcgatg cgtggcaggg actgccggga cacattccga cggagcgcaa ggtgcgctac
     2821 ctgcgggcgc tgattgacgc tggcttccga cacatcgatg cggtgagttt tgtttcgccg
     2881 aaagcggtgc cgcagatggc ggacagcgaa gcggtcctag cccagctcgc tccgcccgac
     2941 gacgtcgaga tcatcggcat cgtggtgaac gaaaagggcg cggagcgcgc gatcgcgacg
     3001 ggggcggtcc gcaccctggg ctatccttgt tcgatctcgg agacgttcct gcgccggaac
     3061 cagaatcaat cgccagagga aaatcgcgcg accctcacca ggattcacga gcaggccgag
     3121 gccagcggcc tcaagatggt cgtctacatt tcgatggcct tcggaaatcc atatcaggac
     3181 ctttggagca tcgagcgcct cgtcgaagca gtgcgaggca tcgctgcgct cgaagtcggc
     3241 tcgatttctc tggcggatac ggttggcatg gcgaaggaga agatgatcgg cgaagtggtg
     3301 gcggcggttg tagccgggat cgatgatccc gggctcgaga tcggagtcca cctgcacagc
     3361 acgcggccgg aagccgcggc caaagtcctg gccgcctacg acgcaggatg tcggcgtttc
     3421 gacgcggcgc tcggcggcct cggtggttgc ccttttgcgc aggatgagct ggttgggaac
     3481 atcccgacag aggaagtgat tcgcgcgcta agccagcgcg gcatccaact cccgctcaca
     3541 cctgagaaag cgacgtccct caacgagcga atcagccagc aattcagcag ttaagcccta
     3601 gcccggggca gcgttggagc ggtttctcgg atattttgct tccactggtg gatgctcgcc
     3661 tgcaaaaatc cccttcatgc cgccgaccgg ttaacaaatc ccttagaaca tgagatcccg
     3721 accctcggag catagactga cgcgttccaa aggcctcctc cgtgttgtcc ttgtggctgg
     3781 cgtcggatgc gtgtctttcc ttggcgcgag cacctacgcg caggagccaa cgccaggagc
     3841 agaggacacg gcggagcgaa tcgtaacggg atcgaacatt ccaacggccg aagaggtctc
     3901 cgcctcggcg ctcgatacgg ttacgacccg ggatatcgcc gaaacgggag gcacctcaga
     3961 tgtgctaac
//
LOCUS       DATMNS010000270        26674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3117600, whole genome shotgun sequence.
ACCESSION   DATMNS010000270 DATMNS010000000
VERSION     DATMNS010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 26674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26674
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3117600"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VNW28_09435"
     CDS             complement(<1..324)
                     /locus_tag="VNW28_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HXA10194.1"
                     /translation="MLTPGQKNVLQFIQAEQRGKGLTPSTREIQKHFGFASQTSVMQY
                     IAALERKGFLQRHARKARALITSAPHSRVVDVPIYGEIPAGMATLAEQNIDGHVALDL
                     DSLNAS"
     gene            complement(373..903)
                     /locus_tag="VNW28_09440"
     CDS             complement(373..903)
                     /locus_tag="VNW28_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10195.1"
                     /translation="MTDYFALLGEARRPWLNLEKLKERYFSLARAAPADAELNEAFRV
                     LSEPKLRLHHLLTLEGAELTAGRPVPSSVAELFWNTGTLLREIERWLLQNKAARSSLA
                     RALLQPERGKLEQRLGKVEEQLRATYQAELAQWPRGEVDWPNEMAGMVERYDSMAYLT
                     RLLEQTAEKRFQLTAA"
     gene            complement(900..1280)
                     /locus_tag="VNW28_09445"
     CDS             complement(900..1280)
                     /locus_tag="VNW28_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HXA10196.1"
                     /translation="MSAASSPPGEIKFKIGNERLVKVTENASRKLTSLLQKQGRAHGA
                     LRVAVIGGGCSGLQYKMDLVDGPANRDIMVESCNVRVVVDPKSALFVSGSVLDFSDDL
                     QKGGFKVTNPNAVAHCSCGESFSA"
     gene            complement(1289..2284)
                     /gene="dusB"
                     /locus_tag="VNW28_09450"
     CDS             complement(1289..2284)
                     /gene="dusB"
                     /locus_tag="VNW28_09450"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HXA10197.1"
                     /translation="MIPPMLPWFSNGRFPLYLAPMAGVSDKIFRQLCKERGADVLVTE
                     FVSSEGVFRRNARTRGYLDFDAIERPIGVQLFGGNAEHMAAAARAVVEWVQPDFIDLN
                     FGCPVNKVVAKNGGSALLKDCPTLGKVADAVVRAVAPLPVTAKIRTGWDDATVNAVSV
                     AQLLESLGMAALAVHGRTRAQGYSGAADWDVIAQVAAAVSIPVIGNGDLFSATDVARR
                     GAESGIAGAMIGRAAMSSPWIFSQTKHYFATGELLPPPEPAEKWALIQRHCELAVAEC
                     GVEDLAMRSMRGRLMAYSKGLPGSKLLREKFQRVSTLPEVEEIARLHLATCLEAA"
     gene            complement(2374..2898)
                     /locus_tag="VNW28_09455"
     CDS             complement(2374..2898)
                     /locus_tag="VNW28_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10198.1"
                     /translation="MNPLADEWSIQHRAEACAVTGRPFAPGENFYTLLFREGAGFRRQ
                     DLSEEAWRQRNENLQPFSFWRARFEPPPAAPPEPLPKENAEELFRRLVAAADRANANA
                     CYVLAAMLERKRILKQIRTENGDRGRILIYEHGKTGDVFVVPDPQLRLDELEEVQMEV
                     MGLMTNAECRMTKE"
     gene            complement(2895..4043)
                     /locus_tag="VNW28_09460"
     CDS             complement(2895..4043)
                     /locus_tag="VNW28_09460"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXA10199.1"
                     /translation="MRLLDRYIIRNFLQPYVYCILGFLSIWLIFDISDNSSTIFDERA
                     PFALVVKFYWTQIPEVLVILLPVALLLALLFSLGRMSRANEIVSMLTAGVSVPRVVFP
                     LLVIGLLTTGATLALNYSLAAHAELARKNFFDQVNRGGVREVVIYGQVFRNRTDHRTW
                     YVSRFRPNSNDFFGVQIVQQDAEENIVRNYIATEAFYEPALKTWRLEKTRMVTYDVAG
                     NITNDVVVPYLLLPGWTETPYRLSSANVRPEFLGISELQDYLRFNADFPETLLAPFRT
                     QLQYRWALPWTCMVVAVMATPLGIGFSRRGILASVAAAIGLVFTMNFLIHLFLALGEG
                     DRVSPMVAAWTPNLIFFLIGLYLLHLRATNREAPSFNPAALWRSLRVA"
     gene            4208..6190
                     /locus_tag="VNW28_09465"
     CDS             4208..6190
                     /locus_tag="VNW28_09465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HXA10200.1"
                     /translation="MIALKDSLASDFVERVRTIFAEDGLLAKARNFEYRPEQQAMAAA
                     VARTLEEERHLVVEAGTGVGKSLAYLVPAILFALEKKKKAIVSTHTINLQEQLLFKDI
                     PIVKKILPVEFEAALMKGRQNYLCPRRLERALQNANELFNTTEQADLARLADWARTTS
                     DGTLSDLPLEPDPKVWTQVCSEAHLCTQKTCGQNTRCFFQQAKRRLLAADVVVINHTL
                     LFMLLGSPEEQEDRAEGYLFPNDFIVFDEAHTIEQVASRQIGIGISQYGLRATIQRLY
                     NARSKKGLFTVTRDAAGVTLAAALVEEADRFFAAIDERANFKKGRECRVREADFVADQ
                     ITGRLAALQARIGEVVRKTDDEFLKSELQELGRRIAEAREGISIFLQQSAREYVYWIE
                     RTGRAAQNITLNAAPIDVAPVLRRMLFRDDCTSVMTSATLAVGRPDLAYFRRRIGADE
                     AEPLQLGSPFDFQKQMKLFVVRKMPDPRDEAYGAALAEWVARFVEETEGRAFVLFTSY
                     RAMQQLALEMEDFFASRKMNLLVQGKGAPRSQLLEQFKNTPRSVLFGTDSFWMGVDVP
                     GDALSNVIITRLPFAVPDHPLIEAKLELIQARGGDAFTEYSLPEAILKFRQGVGRLIR
                     TKSDRGIVVILDNRVVTKTYGRAFLKALPECPVKIL"
     gene            6261..8195
                     /locus_tag="VNW28_09470"
     CDS             6261..8195
                     /locus_tag="VNW28_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10201.1"
                     /translation="MKRFALAAAAAFLAGLAAGRYILPPPRLTTAPVSFPGNSLGEKT
                     TTPDFASWPAITSSARHSSTATAGPASTETIVAAIKNALARPLNRRGYIELSGLIDGI
                     DPKNVRKVLDALQTLPDQREKSTYLAMLVAHWAEGDPQAALAYAQTAGTASDRKSTVP
                     AALRSWAEKDATAARAWVLQMPAGQERDRALLEVVSSLAENDPSAALDMMQTLPAAGG
                     GRQNFYWPIFSRWASTDPVTAAARAVQLPPGSGHDTALQVVASTWANQDAEAAFAWAN
                     TLPPGQGRDSAVQNVLSNWASKDPQRVATVVAALPAGAMREQATASVARQWAQNDPTA
                     ALAWTQELPTGEGQRRALESVLASWAQSDPAAAADYVTTLPAGKAQEDAVRSIARQLA
                     NADVQLALHWAQTLPEGATRQNALSPIVAQWSETNPAAAAEFALSNSSGDTRRNLLED
                     ISRRWAQSDPQAALGWAQNLSDPAACAAVLPNIISAVAESDPARAAQMTLRLSGEAQT
                     AAATSIISQWAGSDPQAASTWAASFPEGTTRVQVFQNLISRWAQSDPTAAASWLGNLS
                     DGPSRDQAVSVFTNRISFSDPQAAVQWAATIGNDSLRNSQVESTARTWLRNDPRNASA
                     WISNSSLPDETKARLLSPGG"
     gene            8350..9288
                     /locus_tag="VNW28_09475"
     CDS             8350..9288
                     /locus_tag="VNW28_09475"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10202.1"
                     /translation="MKDERAAEISHIFSILHKWGVHTLGELAALTPDQLGARLGPVAV
                     RLWEQANGRATRLLRLVRPTEIFAEQLEFEHEIETAEPLLFVLRRFLEQLTLRLGALH
                     LVAQEITLEMIFADPPKDGFAEVNKSRYEHRFHIPDPTNSLEILFRLLQTHLDTFRAD
                     YPIVAVGLAAQATHPARQQFHFFETPLRDPGRLHETLTRLTGLLGRERVGTPVLEDSH
                     RPDAFHLEAFSWELAPAPTELPRMIGPALRRFRTECLPSCDETVLTRQGPYLASGDWW
                     DEKKWQRAEWDLELADGVVCRCEERGDGWEMEGIYD"
     gene            9384..12662
                     /locus_tag="VNW28_09480"
     CDS             9384..12662
                     /locus_tag="VNW28_09480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HXA10203.1"
                     /translation="MYAELHTASAFSFLRGASFPEQLAARAAELELPAVALLDRNGVY
                     GAQRFSVACREHGVRPIIGADLTMECGAILPVLVENRAGYRNLCALLTQAHLRSPKGS
                     CAIRWEELPEFGEGLIPLLGSARAPRAPAGASPDGRAAKTRCEQRSLRRGAANGTRGA
                     CAPRNLADHAQLLLEAFGPDRLYVELQRHLVRGEERVNRQLLDLAAHHRLPLLATNGV
                     EHATPLGREVLDVFTCIREHTHLDAAGPRLTINGERHLKSVAEMKALFRDRPDAIENT
                     LRLAERLQFSLENLGYDFPAYPVPEGHSMDSFLRTITLFGAQQRYAAISRAVQAKLNE
                     ELALIQKLGFAGYFLIVWDIVNFCREHNIMVQGRGSAANSAVCFCLGITPVDPVSNHL
                     VFERFLNENRKGWPDIDLDLPSGERRESVIQEIYRRYGKHGAAMTANVITYRGRSAAR
                     EIGKALNFAPNILDRFSHLFAHGDFPHTLELEAQIEQSGLPRAHPRMPAFVRLYQAIY
                     GLPRHLGQHSGGMIICQDKLSSFVPLENASMPGRVVAQWDKDDCEDLGIVKVDLLGLG
                     MMSVMQDTFEVCRARGRPLDLAHIPKDDPATFALMQQADTIGVFQIESRAQMATLPRM
                     QPKCFYDVVIEVAIIRPGPIQGDMVHPYLNRRARREPVTYFDERLKPILERTLGVPLF
                     QEQMLKIAMIMADFSGAEAEELRRALSFHRSPERMEKVSVKLRAGMERNGVAPEVILK
                     IIQAISSFALYGFPESHAISFAILAYGSAYLKVHRAAEFYASLLNNQPMGFYAPATLV
                     KDAHRHGIRMRPVCVAESEWRCAVESSESVRLGFCVVHGLRQEHAEEIVRQRREQTFR
                     SLEDFKKRLGLTKEELRSLAELGAFNCLAKHRRAALWEVEETLHDDLLGSARDSRAPS
                     GDSPDGRVADTRREQRAFRRGAEKSTRVACAPQHGISPLLPMTLPERVQADYARMNLT
                     TGPHPMKLLRDQLPNVWRATDLAQARHGVTVQIAGNVICRQRPGTAKGFVFISLEDET
                     GVANAIVTPDLFERSRLVITEEPFLVIEGEVQNTDNVVLVKARNIRPLAHAQLAGSES
                     HDFR"
     gene            complement(12693..12899)
                     /locus_tag="VNW28_09485"
     CDS             complement(12693..12899)
                     /locus_tag="VNW28_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10204.1"
                     /translation="MSSRAAEGRREETYNRSDDANAREKEIKGWRRSKKNALVETLNP
                     HWADLSAELFGRWRGPSPRSAGSG"
     gene            complement(12908..13132)
                     /locus_tag="VNW28_09490"
     CDS             complement(12908..13132)
                     /locus_tag="VNW28_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10205.1"
                     /translation="MPTYVYETTDPSRPTRRFEVKQSMKDEPLRVDPETGEPVKRVIS
                     GGYGVHVPGGSTGPSVGSVGGSCGPGCGCH"
     gene            complement(13148..14626)
                     /gene="gndA"
                     /locus_tag="VNW28_09495"
     CDS             complement(13148..14626)
                     /gene="gndA"
                     /locus_tag="VNW28_09495"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF006765.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HXA10206.1"
                     /translation="MKTNQCDIGLIGLAVMGENLVLNMESKGFQVAVFNRTAAVTEKF
                     AANRAKGKNIQPTPTMEEFVGALQRPRKAMIMVKAGAPVDAVIGQLVPLLEKGDVIID
                     GGNSLFTDTQRRCKDLEGRGIHFVGCGVSGGEEGALKGPSLMPGGPRASWEIIAPIFR
                     KIAAVVDGEPCCRYMGSDGAGHYVKMVHNGIEYGDMQLICEAYAILSGVLGLTTNELA
                     DIFADWNRGDLDSYLIDITAQIFRKKDPDTGKPMVDVILDKAGQKGTGIWTLQSAIRQ
                     SVVISTINAAVEARVISSRKDDRVRASKILPQPKVEKFSGQRDALITAVRDALYASKI
                     VSYAQGMELLGAASKEFNWNLNFGDIATIWRGGCIIRAKFLNRIREAYERDPALNNLL
                     LDSYFTDIIQKTQHNWRIAVATAVQQGVAVPAFSASLGYFDSYRQARLSSNLLQAQRD
                     FFGAHTYERVDKPGVFHTEWMHSDASEKVAEPKEAPRRHAAE"
     gene            14704..15105
                     /locus_tag="VNW28_09500"
     CDS             14704..15105
                     /locus_tag="VNW28_09500"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HXA10207.1"
                     /translation="MEILITTSRLQARPNDWSGRAGATLDFWGVVRAREGKCEISGIE
                     YEAHRAMAEHQMKTIAETARAEFGLEEVILEHRIGFVAAGEASLFLRVASAHRAAAYQ
                     GSAWIVAELKQKVPIWKRPVFVRQEETGTAG"
     gene            15102..15689
                     /gene="pgsA"
                     /locus_tag="VNW28_09505"
     CDS             15102..15689
                     /gene="pgsA"
                     /locus_tag="VNW28_09505"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HXA10208.1"
                     /translation="MNWANRLTLGRLALTIVFVLALSSTWVYGRTVALLLFILAGISD
                     YLDGEIARRYGTVTNFGKLIDPLVDKVMMAAAFICLVPLGAVPAWVATIVVARDFLIT
                     GLRLLASSEGTVLAAEKLGKHKTSWQIATVLFFLLLLSARELRPAVEETAWWQNAWRF
                     GGDALLWITIGLTIYSGLGYLWRHRSLILPTGARA"
     gene            complement(15849..16400)
                     /locus_tag="VNW28_09510"
     CDS             complement(15849..16400)
                     /locus_tag="VNW28_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXA10209.1"
                     /translation="MRTSFLALLVLAALAPAAFADAVLRAGDSVDIRISGVPADEQTQ
                     VNNVYTVDANGMVNLPFINKVRAQSLTPAQLASAIENGYKSARIYSNPTITIVMQSQT
                     RFVNVGGAVRAPARIPFTEDMTLLTAISAAGGFNDFADQKRVRLLRGSEAKVYDVRQF
                     RREPGLDPKLQPGDKIEVPQSFF"
     gene            complement(16400..18541)
                     /locus_tag="VNW28_09515"
     CDS             complement(16400..18541)
                     /locus_tag="VNW28_09515"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HXA10210.1"
                     /translation="MAHTEEVRLHFLDYWRVIRVRWVLILLAFLLVVITAAVVTYFQP
                     REYQSSVFIEVKSTADTPRIFGNDGTIPYHDPQLAPTVYQIIQRTGILYPVIEDLKLQ
                     DKWARDGNRPSREQAYQMLREKLDLDEVRNTDLLQISVYDTNPQEAADIANKIVAVYQ
                     EKRVQEEKEILNRAVETMNEEVAKEEQKVEKAGKEVARIRDEEHIIDLNPEGTEDSLT
                     ADNSNVIKQEGEVNDAETNVATLAAKLKQIESLKGDDLMRMMPTLNIQDPIIQKILPS
                     YQEAVANEAQLLNSGLGENHPKVKALRATKAVYVRQLEEQVASVRDGLQRNLNSAQAT
                     HDELQKHLGTINEQQLASKNLSANYTRAKNAYIKEKSLRDNVRLRAQTETMEMAMPRL
                     AVSVKQVAEPPSVAARPRVVLNLALGGLVGLVLGLGLAFFIEYLDTSVKTMDDVETLL
                     DVPVLAIIPQNIRLLFREGGDTPDAEAYRILRTNIEFNRKSLDANTILLSSGGQGEGK
                     STTLANLALVSAQGGYSTLIVDADLRRPVQQTLFDLDEKFGLTNYLTTDLAIEKVIFP
                     TTVPNLFLLPSGVLPADAVGILNSQRMSDLIAELKTRYDIIFFDSPPILGASDASVLA
                     SEVDQTIIVVQHRRFPRAMLTRVKQAIVGVGGSVLGVVLNNVDLRHDQNYYYYTSYAH
                     YYHQRPGDLKATRSQKTMAPARNGAARPEEY"
     gene            complement(18608..19939)
                     /locus_tag="VNW28_09520"
     CDS             complement(18608..19939)
                     /locus_tag="VNW28_09520"
                     /inference="COORDINATES: protein motif:HMM:NF021589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXA10211.1"
                     /translation="MAPKRPSHPRAFFHALLFHGTPGPFPRAAGLIVSLLALTCAPLP
                     LYAQEESSGKTFGAVSSSTGSGEAGPAGLDLGAGIFAPSPLRLSVSVQEGYDDNVYTT
                     HQNPIGSFFTGGSVALDYKFGSARTNLDLQVFGGATYYYFRPLGQTYDINNGLTLNMS
                     HQATPRLGFAASVDLAYQSEPNFDTGVGTNRRSGNYFYTADKFSTSFQWLPRFSTVTS
                     YSLSVINYENSAIGAFEDRFEHTFGNEFRYLALPTTTLVGEYRYEIIDFDTAPRDSTT
                     HFVLAGVDHSFSPHFIVSQRFGVEFRAFDNFGTETSPYGEATLKYSLGERTSVSWNGH
                     YGLDEPDVPGSASRTTFRTGLNVAYAISPRVSATMAVFYEHDQNKSVTTPPFVAPAFN
                     EDLIDLSLGLRYEINRTFTLLANYDRTEDLSDLSFREYSRNRYYVGLSVGF"
     gene            complement(20091..21863)
                     /locus_tag="VNW28_09525"
     CDS             complement(20091..21863)
                     /locus_tag="VNW28_09525"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXA10212.1"
                     /translation="MLGAIVVAIALVGMQVLIGGTRLAYALPVYALLGLGGLFAVVPF
                     RQAKPSPGRACLWSSVFFFGYVLVRAWFSPVPYLARADIFPVLGGLVVYLAVALTFTS
                     ARWRMAILLFLILVALVQVVIGAIQFRNGNNFMLIPFLQRFDYGRRASGFYGCPNHLA
                     GLLEVLGIFCVSLTCWSRWPLWTKLLIGYAGALCYVGLALTGSRGGYWSAGVSLVVVA
                     VLTLVALRKAGSEIFWRTAVVGGVFALMLSAVLFFGFKKSDYLSGRAQNLLDTGNIRL
                     DLWQGAIAQWKLQPAVGTGSGTYLYYGRRFRTSRVQLDPVEVHNDYLQLLAEYGAVGG
                     AAFLIFLGAHLWRGGIAFQELGPRRMPLTSQPLSNRLALNLGALGAVTALAVHSLLDF
                     NLHIPANVLLLAFVFGILANPGFDRENEGAGAIRRVTSGRLGLALLALLLIAGSARYT
                     RAEDYAERSRAALRDERHLEAMRWARQAIARDGQNPEIFFYLAESRIRRAEDSANAMA
                     RDSFYRAALEPLERARALAPNDETFLLALGRVYDALGRFPEAEWMYGRARDWDSKSMV
                     VQKSYAAHLARWSGTKSGVESEPR"
     gene            complement(21957..22604)
                     /locus_tag="VNW28_09530"
     CDS             complement(21957..22604)
                     /locus_tag="VNW28_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10213.1"
                     /translation="MVALAAGLWVAAPAAALGRDRTPKELIEGGLPAAKTIKGATKSE
                     FLAALCAAVRKHRTVAAAITDVAVGTRREFTGEIVGTVLRCGAKGDCKFVGSIVSAAI
                     SANDNAATTVADAAIATAPDCADAIQSVVSHAASGGGERADSDAAGPDNQNPLSDAKS
                     KDTGEGFDPHEQLILVCDDGTQRTVRQSQLDEYLQSHQGAVVGLCPPTPSPTANK"
     gene            complement(22749..22819)
                     /locus_tag="VNW28_09535"
     tRNA            complement(22749..22819)
                     /locus_tag="VNW28_09535"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22785..22787),aa:Gly,seq:tcc)
     gene            22812..23672
                     /locus_tag="VNW28_09540"
     CDS             22812..23672
                     /locus_tag="VNW28_09540"
                     /inference="COORDINATES: protein motif:HMM:NF025729.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HXA10214.1"
                     /translation="MPARDEAAAPAPACQVISENVGGLCAAIVAAQRRLPLSPNLPIA
                     PTPASNCHMKSLHLFGIASLALLATSRADLTIVQKVDGAGGGVHELTLKVKGDKARVD
                     INPQITTIIDAKTGEVTTLLNDKKTVMRISGEKAKAMAEMAKSFAKEESPTQALPKST
                     GKKETINGYETEEYVTDSPKYHASYWVAKSYPNYQSILQQMSIMQQGAFAGLTKGLPD
                     YHALPGLPLRTQVRIPGQDDVTSTIESLKLDPLPDSDFSIPAGYSEIKMPDFIGGKQP
                     PGKPDDPGNE"
     gene            23669..24262
                     /locus_tag="VNW28_09545"
     CDS             23669..24262
                     /locus_tag="VNW28_09545"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXA10215.1"
                     /translation="MTDDAPRLILASASPRRRQLLTEAGFAFDIVAPLVDENLSDHFT
                     LREVTCWNALRKGLAVARAHPEQVVLAADTLVGLGHQIIGKPADRAAAVRVLRRLSGR
                     THIVVSAVFIAHLRRGKSESFADTSRVVFKRLSDRMIADYLAVIDPLDKAGAYAAQGH
                     GSQIIARIIGSRSNVIGLPLEKTRAALARFDLHRRSA"
     gene            complement(24266..24811)
                     /locus_tag="VNW28_09550"
     CDS             complement(24266..24811)
                     /locus_tag="VNW28_09550"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HXA10216.1"
                     /translation="MSDVAPIPKMLRPQRRVRYLKIIAIFKILQGAVLFSIGISLLFL
                     HSRTLWLNGISEWVDGELMVAHSRAMLYLLSKWQDVVAGGLLQVTGLVALVSAAVLCT
                     EGIGVYLQKRWAEVLMVVATGALIPFEVRHAWRSPGAVALILVAANCFIVWFLYRVLR
                     REKCEELAVPADDERTPVESR"
     gene            complement(24843..25238)
                     /locus_tag="VNW28_09555"
     CDS             complement(24843..25238)
                     /locus_tag="VNW28_09555"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXA10217.1"
                     /translation="MKTRRFDHIDLRVREIAAARPFYARILPELGFTIASSSGEWSIW
                     QAPGDGPVEFFGFTEAPRHQPNDNRIAFWAESQAEVDRLAEIVRQAGGQNLEGPELWR
                     EYSPGYYAFFFEDPSGNKLEICCRQSRAA"
     gene            complement(25244..26236)
                     /locus_tag="VNW28_09560"
     CDS             complement(25244..26236)
                     /locus_tag="VNW28_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HXA10218.1"
                     /translation="MNAAPQHLDRPVGDFVRKDFAQLQESMTARQALDAVREEGIGEK
                     IIYFYVVDEENRLVGVLPTRRLLTAPLDQRIGDLMIKRIIAIPQTATLLEACEFFVLH
                     KFFAFPVVDTERRILGLIDVGVFTEEVFDIAERAQHEDVFETIGFYVAQVRDAGPLRA
                     FRYRFPWLLATIVGGTACALLASAFQLTLAQSLVIAFFLTLVLGLGESVSMQSMTVTI
                     QALRVARPTPRWYLGALRREVAVSLLLGAACGLLVALIVWFWLGEGWPALVIGSSILS
                     SLVMACLLGLSIPSLLHALRLDPKIAAGPLTLALADLLTLLFYLSIARWLLQPA"
     gene            26331..26636
                     /locus_tag="VNW28_09565"
     CDS             26331..26636
                     /locus_tag="VNW28_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10219.1"
                     /translation="MTRRIKRIAPLQAGKMLAVVYGCMGLIFLPFFALAGTLGAFAQH
                     AQPNQAAPAALVGGLLVGMGFFFPIIYAAMGFIFGVVGAALYNLVARWIGGFEVEVE"
BASE COUNT         5388 a         8221 c         8096 g         4969 t
ORIGIN      
        1 cgaggcattg agcgaatcga gatcgagcgc gacatgacca tcgatgtttt gctcggcgag
       61 ggtcgccatg cccgccggga tctcgccgta aatcggaacg tccaccaccc ggctgtgcgg
      121 cgcggaagta atcaaggcgc gcgccttgcg tgcgtggcgc tggagaaaac ctttgcgctc
      181 cagcgcggcg atgtattgca tgaccgaggt ctggctggcg aagccgaagt gtttctggat
      241 ttcacgcgtg ctcggggtga gccctttgcc gcgttgttcc gcctggatga attgcaggac
      301 gttcttctgc ccgggcgtga gcatatgaac atgttggcga aaacctccgc gcgcgcaagc
      361 gccaaatgcc aatcaggcag ccgttaactg aaatctcttt tcggcggtct gctccagcag
      421 ccgggtgaga taggccatcg aatcgtagcg ctccaccatc cctgccattt cgttaggcca
      481 atcgacctca ccgcgcggcc attgcgcgag ctccgcctgg taggtggccc gcagctgctc
      541 ctccaccttg cctaaccgtt gctccaactt cccccgctcc ggctgaagca gcgcgcgcgc
      601 gaggctgctt cgcgccgcct tgttctgcag cagccaccgc tcgatctcgc gcagcaacgt
      661 gccggtgttc cagaaaagct ccgccaccga cgacggaacc gggcgaccgg cggtgagctc
      721 ggcgccttcc agcgtgagca gatgatgcag gcgcaacttc ggctcgctca gcacgcgaaa
      781 cgcttcgttc aattccgcat ctgccggtgc ggcgcgggcc agcgagaaat atctttcctt
      841 cagcttttcg agattgagcc agggccgccg cgcttcaccg aggagcgcga agtaatcggt
      901 catgccgaga agctttcgcc gcaggaacaa tgcgcgaccg cattcggatt cgtcaccttg
      961 aagccgcctt tttgcaggtc gtcgctgaaa tcgaggaccg acccgctgac gaaaagtgcg
     1021 ctcttcgggt cgaccacgac ccggacgttg caagactcga ccatgatatc gcggttcgcc
     1081 gggccatcga ccaaatccat tttgtattgc agtcccgaac agccgccgcc gatcaccgcc
     1141 acccgcaagg caccatgcgc gcgtccctgt ttttgcagga gcgaagtcag cttgcgcgag
     1201 gcgttctcgg tcactttgac gaggcgctcg ttgccgattt tgaatttgat ttcgcccggg
     1261 ggcgaagaag cagcggacac gagggaattt acgccgcttc gagacaggtt gccaaatgga
     1321 ggcgcgcgat ttcctccacc tccggcaaag tggagacgcg ctgaaatttt tcacgcagca
     1381 gtttgctccc cggcaatccc ttggaatagg ccatcaaacg gccccgcatc gaacgcatcg
     1441 cgaggtcttc gacgccgcat tccgcgacgg ccagttcgca atgccgctgg atgagcgccc
     1501 atttttccgc cggctccggc ggcggcaaaa gttcgccggt cgcgaagtaa tgtttcgtct
     1561 gcgaaaaaat ccacggagaa ctcatcgcgg cgcgcccgat catggcgccg gcgatgccgg
     1621 attcggctcc gcgtcgggcc acgtcggtcg cggaaaagag atcgccgttg ccgatcaccg
     1681 ggatggagac ggcggccgcg acctgggcga tcacgtccca atcggccgcg ccggaatagc
     1741 cctgggcccg ggtgcgaccg tggaccgcga gcgccgccat gcctaacgac tccaaaagtt
     1801 gcgctacgct caccgcattg accgtcgcat cgtcccagcc ggtgcgaatc ttggccgtga
     1861 ccggcaaggg agcgaccgcc ctaacgactg cgtcagctac cttgcctaac gttgggcaat
     1921 ccttgagcag ggccgagccg ccgttcttcg cgaccacctt gttcactggg cagccgaagt
     1981 tcaggtcgat gaaatccggc tgcacccatt cgaccacggc ccgcgcggcc gccgccatgt
     2041 gttccgcgtt gccaccgaaa agctggacac cgatcggtcg ttcgatggcg tcgaagtcga
     2101 ggtagccgcg ggttcgggca ttgcgccgga agacgccttc cgaggagaca aactctgtca
     2161 ccagaacatc ggcgccgcgc tccttgcaaa gttgccggaa aattttatcc gagacgccgg
     2221 ccatcggggc caggtagagc gggaaacgcc cgttggaaaa ccagggaagc atgggcggga
     2281 tcaaatgact aatttcgaac gacgaattcc gaaggcctga cgaaacccgg aggccagaag
     2341 gttgcagaag gcggttttgt cattcggcat gggtcattcc ttcgtcattc ggcattcggc
     2401 attcgtcatt aaccccatca cctccatctg cacctcttcc aactcgtcca gccggagttg
     2461 cggatcgggc acgacgaaga catccccggt tttcccgtgc tcgtagatca ggatgcggcc
     2521 gcggtcgccg ttctcagttc ggatttgttt gaggatccgt tttctctcca gcatggccgc
     2581 gagcacgtaa caggcattgg cgttggcgcg atccgcggcc gcgaccaggc gtcgaaaaag
     2641 ctcctccgca ttctccttcg gcaacggctc cggcggtgcg gcgggcggcg gttcgaaacg
     2701 ggctcgccaa aaagaaaaag gctgcaagtt ttcgttgcgc tgccgccagg cttcttcgct
     2761 cagatcctgc cggcgaaaac cagcgccctc gcgaaaaagc agggtgtaaa aattctcacc
     2821 cggcgcaaac gggcggcccg tcaccgcgca cgcttcggcc cggtgctgga tcgaccattc
     2881 gtccgccaga ggattcatgc cacgcgcaaa ctacgccaga gcgccgccgg attaaagctg
     2941 ggcgcttcgc ggttcgtcgc gcgcagatgg agcaggtaga gaccgatcag aaaaaagatc
     3001 aaattcggcg tccaggccgc gaccatcggc gagacccggt cgccttcgcc gagcgcgaga
     3061 aaaagatgaa tcaaaaaatt catggtgaag accagcccga tcgccgccgc cacactggca
     3121 agaatgccgc gccgggagaa accgatccca agcggcgtcg ccatgaccgc cacgaccatg
     3181 cacgtccagg gcagcgccca acggtattgc aactgagtgc gaaagggggc gaggagggtt
     3241 tcgggaaaat cggcgttgaa acgcaagtaa tcctgcagct cggagatgcc gagaaactcc
     3301 gggcgaacgt tggcgctgct caggcgataa ggcgtctcgg tccagccggg gaggagaaga
     3361 taggggacca ccacgtcgtt cgtgatgttg ccggcgacgt cgtaggtcac catcctcgtc
     3421 ttttccagcc gccaggtttt gagtgccggc tcgtagaaag cctcggtcgc aatatagttc
     3481 cggacgatat tctcctccgc atcctgctgc acgatttgca cgccgaagaa atcattcgag
     3541 ttcggccgga aacgcgagac gtaccaggtg cgatggtcgg tccggttgcg aaaaacctgt
     3601 ccgtagataa ccacctcgcg cactccgccg cgattgacct ggtcgaagaa attcttccgg
     3661 gccagctcag cgtgggcggc gagtgagtaa ttcaaagcga gcgtcgcgcc agtggtgagg
     3721 agtccgatga cgagcaaagg gaagacgacc cggggaacac ttacgcccgc cgtcaacatc
     3781 gagacgatct cattcgcgcg cgacattcgg cccagcgaaa aaaggagcgc caggagcaaa
     3841 gcaaccggca acagaatcac cagaacctct gggatctggg tccagtaaaa tttcacgacc
     3901 agcgcgaacg gtgcgcgctc atcgaagatc gtcgaactgt tgtcgctgat gtcgaagatg
     3961 agccagatcg aaaggaagcc gagaatgcaa taaacatacg gctggaggaa gtttcgaatg
     4021 atgtagcggt cgagcagcct catgggggaa atttcgaaat ccgaatgtcg cagtgcgaaa
     4081 ccggcacgaa actgaaaact cgaatcttca aaaaatgcgc gtccgacgcc ggaggttttc
     4141 gattttgaag attggtcatt cggatttcga tattcgatat tcgaatttcc cgcgtaactt
     4201 cgcgggaatg attgcgctca aagattccct cgcgagcgac tttgtcgagc gcgtgcggac
     4261 gatttttgcc gaggacggcc tgttggcgaa agcgcgcaac ttcgaatatc ggcccgagca
     4321 acaggcgatg gccgcggcgg tcgcgcgcac gctggaggag gaacgccatc tcgtcgtcga
     4381 ggccggcacc ggggtcggca agtcactcgc ttacctcgtg ccggcgatcc tcttcgcgct
     4441 cgagaaaaaa aagaaggcga tcgtctcgac ccacacgatc aacctgcagg aacagctcct
     4501 cttcaaggac atcccgatcg taaaaaaaat cctgccagtc gagttcgagg ccgcgctcat
     4561 gaagggccgg cagaattatc tttgcccccg gcggttggag cgcgccctgc aaaacgcgaa
     4621 cgaactcttc aacaccaccg agcaggccga cctagcccgg ctggccgact gggcacgcac
     4681 gacaagcgac gggacattaa gcgacctgcc gctcgagccc gatccgaaag tctggacgca
     4741 agtctgtagc gaggctcacc tctgcacgca gaagacctgc ggacaaaaca cgcgctgttt
     4801 tttccagcag gcgaagaggc gcctgctcgc ggccgacgtg gtggtgatca atcacaccct
     4861 tctcttcatg ctcctcggca gccccgaaga acaggaggat cgcgcggaag gctacctctt
     4921 ccctaatgac ttcatcgttt tcgacgaagc gcacaccatc gaacaggtgg cttcgcgaca
     4981 gatcgggatc gggatttcgc agtacggttt gcgcgccact atccagcggc tttacaacgc
     5041 ccgctccaag aaagggcttt tcaccgtcac gcgtgacgcg gccggggtga cgctggccgc
     5101 tgcgctcgtc gaggaagcgg accggttttt cgccgccatc gatgaacgcg ccaatttcaa
     5161 gaaaggccgc gaatgccggg tgcgggaggc cgatttcgtc gccgaccaga tcaccggacg
     5221 attggctgcg ctgcaggcgc ggatcggcga agtggtgcgc aaaacggacg atgaatttct
     5281 caaatccgag ctgcaggagc ttggacgacg gattgccgaa gcgcgtgaag ggatctcgat
     5341 ttttctgcaa cagagcgcgc gggaatacgt ctactggatc gagcgcacgg gaagggccgc
     5401 gcagaatatc acgctcaacg cggcgccgat cgatgtcgcg ccggtcctgc ggcggatgtt
     5461 gttccgtgac gattgcacga gcgtgatgac aagcgcaacg ctcgcggtcg gccggccgga
     5521 tctcgcctac ttccggcggc ggatcggggc ggatgaagcc gaaccgctgc agctcggcag
     5581 cccgtttgat tttcaaaaac aaatgaaact gttcgtcgtg cgcaaaatgc ccgacccgcg
     5641 cgacgaagcc tacggcgccg cgctcgcgga atgggtggcg cgcttcgtcg aggagacgga
     5701 agggcgcgcg tttgtgctct tcaccagcta ccgcgcgatg cagcaactgg cgctggagat
     5761 ggaggatttt ttcgcgagcc gcaaaatgaa tttgctcgtg cagggcaaag gcgcgccgcg
     5821 cagccagctc ctcgagcaat tcaaaaacac gccgcgcagc gtgctcttcg ggaccgacag
     5881 tttttggatg ggcgtcgatg tcccgggcga tgcgctctcc aatgtcatca tcacacggct
     5941 gccgttcgcc gtccccgatc atccgctgat cgaggcgaag ctcgaactca ttcaggcgcg
     6001 cggcggcgac gccttcacgg aatactcgtt gccggaagcg atcttgaaat tccggcaggg
     6061 cgtcgggcgc ctgatccgga ccaagagtga ccgcggcatc gtggtgattc tcgacaaccg
     6121 ggtcgtgacg aaaacctacg gacgggcctt tctcaaagca ttgccggagt gcccggtgaa
     6181 gattttgtga gacggtgggg cttgctcaac tctgcgcgac gtgcccagat ttcccgacgg
     6241 gaacagttac ttttcttgcc atgaagcgtt ttgctcttgc cgccgcggct gcctttctcg
     6301 ctggtctggc cgccgggcgc tacatcctgc cgccgccccg gctcacgacc gcgcccgttt
     6361 cctttccggg caattcgtta ggcgaaaaaa cgacgacgcc cgatttcgca tcctggcccg
     6421 cgatcacttc ctctgcgcgc cattccagca ccgccacggc cggacctgcc tccacggaaa
     6481 caatcgtcgc cgccatcaaa aatgcgctgg cgcgacccct aaaccgacgc ggctacatcg
     6541 agctgagcgg actgatcgat ggcatcgacc ccaaaaatgt gcgcaaggtg ctcgacgcgc
     6601 tgcaaactct gcctgaccaa cgcgagaaat cgacctacct ggcgatgctc gtcgcgcact
     6661 gggcggaggg cgatccgcag gccgccctcg cttatgcgca gaccgccggc accgcttcgg
     6721 atcgcaaatc gacggtcccg gccgccctcc ggtcctgggc agagaaggat gcgaccgccg
     6781 ccagggcctg ggtcctgcaa atgccagccg gccaggaacg cgatcgcgct ctgctagagg
     6841 tggtctcctc gttagcggaa aacgatccat cagccgcgct cgacatgatg caaacgttgc
     6901 ccgccgccgg cggcggacgg caaaattttt actggccgat tttttctcgt tgggccagca
     6961 ccgacccggt gacagccgcg gcgcgcgccg tgcaactgcc gcccggttcc ggacacgata
     7021 ccgccctcca ggtcgtcgcc tccacctggg cgaatcaaga cgccgaggcc gctttcgcgt
     7081 gggcgaacac tttgccgccc ggccagggac gcgacagtgc tgtgcaaaat gttttatcaa
     7141 actgggcgag taaggatccg cagcgcgtcg ccaccgtcgt cgcggccctg ccggccggtg
     7201 cgatgcgcga acaggcgacc gccagcgtcg cgcgccaatg ggcgcaaaac gatcccacag
     7261 ccgcgctcgc ctggacgcag gaacttccaa ctggcgaagg gcaacgccgc gcgctggaaa
     7321 gcgtgctggc cagttgggcg cagagcgatc cggcggcggc ggcggattac gtgacgacgc
     7381 tgccagccgg taaagcgcag gaggacgcag tgcgatcgat cgcccggcaa ctcgcgaatg
     7441 ccgatgtgca actggctttg cattgggcgc aaacacttcc cgagggcgcg acgcggcaaa
     7501 acgcgctcag tcccatcgtc gcccaatgga gcgaaacgaa tccggccgcc gccgcagagt
     7561 tcgccttgag caatagcagc ggagacacgc gccgaaattt gctcgaagat atttcgcgcc
     7621 gctgggcgca gagcgatccg caggccgctc tgggttgggc gcagaatttg tccgaccccg
     7681 ccgcctgcgc tgccgtcctg ccgaacatca tttccgcggt cgccgaaagc gatccagcga
     7741 gagccgccca gatgacgctg cgactctccg gcgaagcgca aaccgcggcg gcgacttcca
     7801 ttatctcgca atgggcgggg agcgatccgc aggccgccag cacgtgggcc gcctcgtttc
     7861 cagaaggaac aacgcgcgtc caggtttttc aaaatttgat cagtcgctgg gcgcaaagtg
     7921 atccgaccgc ggccgcgagc tggctgggga atttatccga tggcccttcg cgcgaccagg
     7981 cggtgagcgt tttcaccaac cggatcagtt tctccgatcc gcaagccgca gtccagtggg
     8041 cggcaacgat cggcaacgat agcctgcgca acagtcaggt agaatccacc gcccgcacct
     8101 ggctgaggaa cgatccgcgc aacgcctccg cctggatctc caattcgtcg ttgccggacg
     8161 agaccaaggc gcggttgctg agcccgggcg gctagggagc cgggatctcg gtagagcgag
     8221 actccgtcgc gctggggatg gcgcgacgcg cggcgaaaga cgtcttctcc ccggctcgac
     8281 ggagtctcgc cctcccaaag gaattatgtt cccttgaaca tagtccttcc cggctctaat
     8341 ttttcgacca tgaaggacga acgcgccgcg gaaatttcgc acattttctc gatcctccac
     8401 aagtggggcg tccacacgtt aggcgaactc gccgccctca ccccggatca actcggcgcc
     8461 cggcttggcc cggtcgcggt gcggctctgg gaacaggcca acggtcgggc gacgcggctc
     8521 ttgcgactcg tcaggccaac ggaaattttc gccgaacaac tcgagttcga gcacgagatc
     8581 gagacggcgg aaccgctcct ttttgttctc cggcgctttc ttgagcaact caccctgcgc
     8641 ctcggcgcgc tccatctcgt cgcgcaagag atcacgctcg aaatgatttt tgccgatccc
     8701 ccaaaggacg gattcgctga ggtgaacaag tcccgctatg aacaccgctt ccacattccc
     8761 gatccgacca actcgctgga aattttattc cggttgctcc agacccatct cgacactttt
     8821 cgagcggact acccgatcgt ggccgtgggg ctcgccgcgc aggcgaccca cccggcgcgg
     8881 caacagtttc attttttcga gacgcccttg cgcgacccgg gccgactgca cgaaaccctc
     8941 acccggctca cgggattgtt aggccgagag cgggtcggga cgccggtcct ggaggattca
     9001 catcggcccg atgcctttca tctcgaggcg ttttcctggg agttggcgcc cgcacctacc
     9061 gaattgccga ggatgatcgg tccggcattg cgccgcttcc gcaccgaatg tttgccctca
     9121 tgcgatgaga cggtcctgac acgacagggt ccttatctcg cctccggcga ttggtgggac
     9181 gaaaagaaat ggcaacgcgc ggaatgggat ttggagcttg ccgacggcgt ggtttgccgc
     9241 tgcgaggaac gcggggatgg ctgggagatg gagggcattt atgactgaaa aattcggccg
     9301 attacgagca cgatcatgag caggagcatg attttgaaaa cgacctctac ctcgtgctcg
     9361 tgatcgtgat cgatcgacct cgtatgtacg ccgaattaca caccgccagc gctttcagtt
     9421 ttctccgcgg cgcttccttt cccgagcagc tcgccgcgcg cgcggcggag ttggagcttc
     9481 cggcggtcgc cctgctcgat cgcaacggtg tctacggcgc gcaacgtttt tccgtcgcct
     9541 gtcgcgagca cggagtgcgc ccaatcattg gtgccgactt aacgatggag tgcggcgcga
     9601 ttctgccggt attggtcgag aatcgcgccg gctatagaaa tctctgcgca ctgcttaccc
     9661 aggcgcatct gcgcagtccg aagggaagtt gcgcgattcg gtgggaagaa ttgccggagt
     9721 ttggagaagg gttgattccc ctgctgggga gcgcacgcgc cccgcgcgct ccggccggcg
     9781 cctcgccgga cggccgcgct gccaagacgc gctgcgagca aagaagtttg cggcggggcg
     9841 ccgcaaacgg cacccgaggc gcgtgcgctc cccggaatct cgcggaccac gcgcagcttc
     9901 tgctcgaggc gtttggcccc gaccgcctct atgtcgaact gcaacgccat ctcgtgaggg
     9961 gcgaagaacg cgtgaaccgc cagctgctcg atctcgcggc acatcatcgt ctcccgctgc
    10021 tcgcgaccaa cggcgtagag cacgccacac ccctgggccg cgaagtgctc gacgtcttca
    10081 cctgcatccg cgaacacacc catctcgatg ccgcgggtcc tcgcctaacg attaatgggg
    10141 aacgccattt aaagagcgtc gcggaaatga aagcgctctt tcgcgatcgg ccggacgcga
    10201 ttgaaaacac cctgcgcctc gcggagcgcc tccagttttc cctcgagaat ctcggctacg
    10261 atttccccgc ttacccggtg ccggagggcc attcgatgga ttccttcctg cgcacgatca
    10321 cgcttttcgg cgcgcaacag cgctacgccg cgatcagccg cgcggtccag gcgaagctca
    10381 acgaggaact cgcgctcatc cagaagctcg ggttcgccgg atattttttg atcgtctggg
    10441 acatcgtcaa tttttgccgc gagcacaaca tcatggtgca ggggcgcggg agcgcggcga
    10501 acagcgcggt ttgtttttgc cttgggatca cgccggttga tccggtgagc aaccatctcg
    10561 tcttcgagcg tttcctgaat gagaaccgga aaggctggcc cgacatcgac ctcgatctcc
    10621 cgagcggcga gcggcgcgaa agcgtcatcc aggaaattta tcggcgctac ggcaaacacg
    10681 gcgccgcgat gaccgcgaac gtgatcactt atcgcggccg gagcgcagca cgcgaaatcg
    10741 gcaaggcact caacttcgcg ccgaacattc tcgaccgctt ctcgcacctc tttgcgcacg
    10801 gcgatttccc gcacaccctc gaactcgagg cgcagatcga gcaatccggt ttgcccaggg
    10861 cgcatccgcg catgcccgcg ttcgttaggc tataccaggc gatttacggc ttgccccgcc
    10921 acctcgggca gcactccggc gggatgatca tctgccagga caaactgagc tcgttcgtcc
    10981 cgctggagaa cgcgtcgatg ccgggccggg tcgtcgcgca gtgggacaag gatgattgcg
    11041 aagatctcgg gattgtgaaa gtcgacttgt taggcctggg catgatgtcg gtcatgcagg
    11101 atacgtttga ggtttgccgg gcgcgcgggc ggccgctcga cctggcccac atcccgaagg
    11161 atgaccccgc gactttcgcg ctgatgcagc aggccgacac gatcggcgtt ttccaaatcg
    11221 agagccgtgc ccagatggcg accctgccgc ggatgcagcc aaaatgtttt tacgacgtgg
    11281 tcatcgaggt cgcaatcatc cgcccggggc cgatccaggg ggacatggtg catccttatc
    11341 tgaaccggcg cgcgaggcgc gagccggtga cctatttcga cgagcggctg aaaccgatcc
    11401 tcgagcgaac gttaggcgtg cctttgtttc aggagcaaat gctcaagatc gcgatgatca
    11461 tggccgattt ttccggcgcg gaagcggaag agctgcgccg cgcgctcagt ttccatcgct
    11521 cgccggaacg gatggaaaag gtgagcgtca aactgcgggc cgggatggaa agaaatggcg
    11581 tcgccccgga agtgattctc aagatcattc aagcgatcag ctcgtttgcg ctttacgggt
    11641 ttccggagtc gcacgcgatc agtttcgcca tactcgccta cggaagcgcg tatctgaaag
    11701 tgcatcgcgc ggcggaattt tatgccagtc tgctcaacaa tcagccgatg ggattctatg
    11761 ccccagccac gctggtgaag gacgcgcacc gccatgggat ccggatgcgg ccggtttgcg
    11821 tggcggaatc ggagtggcgc tgcgcggtcg agtcgagtga gagcgtacgg ctgggatttt
    11881 gtgtggtgca tgggctgcgg caggaacatg cggaggagat cgtcaggcaa cgaagggagc
    11941 agacatttcg ttcgctcgaa gatttcaaaa agcgacttgg cctaacgaag gaggaactgc
    12001 ggagtttggc ggaattggga gcgttcaatt gcttggcgaa gcatcggcgg gcggcacttt
    12061 gggaagtgga agagacgttg cacgacgatc tgttggggag cgcacgcgac tcgcgtgctc
    12121 catctggcga ctcgccggat ggtcgcgtgg ctgatacccg ccgggagcaa agagcttttc
    12181 ggcgaggcgc cgaaaagagc acgcgagtcg cgtgcgctcc ccagcacggg atctcgccgc
    12241 tcctcccgat gacgctgcca gagcgggtgc aggccgatta cgcgcgcatg aacctgacga
    12301 ccggaccgca tccgatgaag ttgctgcggg atcaattgcc taacgtttgg cgggccaccg
    12361 atctggcaca ggcgcggcac ggcgtcacgg tacaaatcgc cggcaacgtg atttgccggc
    12421 agcgccccgg gacggcaaag ggatttgtct tcatcagcct ggaggatgaa accggggtcg
    12481 cgaatgcgat tgtcacgccc gatttgtttg aacggagccg tttggtgatt acggaggagc
    12541 cgtttctcgt catcgaaggc gaggtgcaaa ataccgacaa cgtcgtgctg gtgaaagcgc
    12601 ggaacattcg cccgctggcc cacgcccaac tcgccgggag cgagtcgcac gattttcgtt
    12661 aggcaacaaa acgcgtgcca agctcaaacc tgtcatcccg agccggcgga gcgcggcgaa
    12721 ggacctcgcc agcgaccaaa aagctcggct gacaaatcgg cccagtgcgg attgagcgtt
    12781 tcaacgagcg cgttcttctt gctgcgacgc cagcctttga tttccttctc gcgcgcgttg
    12841 gcgtcgtcgc tgcggttgta ggtttcctct cgccgacctt cggcggctcg ggatgacaag
    12901 agttttttta atgacagccg cagccggggc cgcaggaacc gccgacagaa ccgaccgacg
    12961 gaccggtcga tccgccgggc acatggaccc cgtagccgcc ggaaatgacg cgcttgaccg
    13021 gttcgcccgt ttccggatca acccgcaggg gctcatcttt catgctctgc ttgacctcga
    13081 aacgccgcgt cggcctggaa ggatcggtcg tttcgtaaac gtaggttggc ataacgaatc
    13141 gggcgaccta ctcggccgcg tgccgccggg gcgcttcctt gggttcggct actttttcgc
    13201 tggcatccga gtgcatccac tcggtgtgaa aaacgcctgg cttgtcgacc cgctcgtagg
    13261 tgtgggcgcc gaagaaatcg cgctgcgcct ggagcagatt ggaagagagg cgcgcctggc
    13321 ggtaactgtc gaagtagcct aacgacgcgc tgaaggccgg gaccgccacc ccctgctgga
    13381 cggcggtcgc gaccgcgatc cgccagttgt gctgcgtctt ctggatgatg tcggtgaagt
    13441 aggaatcgag gagcagattg ttcagggccg gatcgcgctc gtaggcttcg cggatacggt
    13501 tgaggaattt cgcccggatg atgcatccgc cccgccagat ggttgcgatg tcgccgaagt
    13561 tcaggttcca attgaactcc ttgctggctg cgcctaacaa ttccattccc tgggcgtagg
    13621 agacgatctt cgaggcgtaa agggcgtcgc gcacggcggt gatgagcgca tcgcgctgac
    13681 cgctgaattt ctctaccttg ggctgaggca gaatcttcga ggccctgacc cggtcgtctt
    13741 tccgcgaaga aataacgcgc gcttcgaccg cagcattgat cgtggagatg acgaccgact
    13801 ggcggatggc cgattgcagc gtccaaatgc cggtgccttt ctggcccgcc ttgtcgagga
    13861 tgacatcgac catcggtttg cccgtgtcgg gatctttttt gcgaaaaatc tgcgcggtga
    13921 tgtcaatcag gtaactgtcg agatcgccgc ggttccagtc cgcgaaaatg tccgccagtt
    13981 cgttagtcgt aaggccgaga accccgctca ggatggcata ggcttcgcag atcaactgca
    14041 tgtcgccgta ctcgatcccg ttgtggacca tcttgacgta atggccggcg ccgtccgacc
    14101 ccatgtagcg gcagcagggc tcgccatcga ccaccgcggc gatcttgcgg aaaatcggcg
    14161 cgatgatttc ccacgaggcg cgcggaccgc cgggcatgag cgaggggcct ttgagtgcgc
    14221 cttcctcgcc cccggaaacg ccacagccga caaaatgaat gccgcgccct tccagatcct
    14281 tgcagcgccg ttgcgtatcg gtgaaaaggg agttgccgcc gtcgataatg acgtcgcctt
    14341 tctcgagcag cgggacgagc tgaccgatga ccgcgtcgac tggcgcgccg gccttgacca
    14401 tgatcatcgc cttgcggggt cgttgcaacg cgcccacgaa ttcctccatc gtcggggtcg
    14461 gctggatgtt ttttcctttc gcgcgattcg ccgcaaattt ttccgtgacg gcggcggtgc
    14521 gattgaagac agcgacctgg aagcccttcg actccatgtt gaggacgagg ttttcgccca
    14581 tgacggcgag gccgatgagg ccaatatcac attgattcgt tttcattttc gttttctccg
    14641 gaatgcgtca ccatggcgta gcaggagcgt gcggacgcgc tgaagcagat ggcaacttgg
    14701 cacttggaaa tccttatcac aaccagccgg ttgcaagcgc gcccgaatga ctggagcggt
    14761 cgagccggcg cgacgctcga tttctggggc gtggtgcggg cgcgggaggg gaaatgcgag
    14821 atctccggga tcgaatatga agcgcaccgc gcaatggcgg agcaccagat gaaaacgatc
    14881 gcggagaccg cgcgggccga gttcggcttg gaggaagtga ttctggagca tcgcatcggc
    14941 tttgtggccg cgggcgaagc ctcgcttttc cttcgcgtcg cgagcgcgca tcgtgctgcc
    15001 gcctaccagg gaagcgcctg gatcgtggcc gagttgaagc aaaaagtgcc aatctggaag
    15061 cgccccgttt tcgttaggca agaggagacc ggaaccgcgg gatgaactgg gccaatcgac
    15121 tcactcttgg ccgactggcg ctgacaattg tcttcgtcct cgcgctcagc tccacctggg
    15181 tttacggccg caccgtcgca ctccttctct ttattctcgc cggaatcagc gattatctcg
    15241 acggcgaaat cgcccggcgc tacgggaccg tgacgaactt cgggaaactc atcgatccgc
    15301 tggtcgacaa agtgatgatg gctgccgcct tcatctgtct ggtgccgctc ggagcggtgc
    15361 ccgcctgggt agcgacgatc gttgtcgcgc gcgacttcct catcaccggc ctgcgtctcc
    15421 tcgcttcgtc ggaaggaacc gtcctcgcgg ccgagaagct cgggaaacac aaaacctcgt
    15481 ggcaaatcgc gacggtcctc ttttttctcc tccttctctc ggcccgcgag ctccggcccg
    15541 cggttgagga aaccgcctgg tggcaaaacg cctggcgctt cggaggggac gccctgctct
    15601 ggatcacgat cggcctcacg atctactccg ggctgggcta cctctggcgg catcgtagcc
    15661 tcattctgcc cacgggggcc cgtgcctaac gacagcgctg gcgaggtcct ctatcccctg
    15721 cagggacgcg atcaacgcgc ccggccctgg tcgagcggca gttcgtccct tcaaagggac
    15781 gcgcttccgt atccccagga cgagtcccgc ggagaagcgc cgtgttcact ttgcaatcac
    15841 ccaaggcact aaaaaaagct ctgcggcact tcgatcttgt caccgggctg cagcttcgga
    15901 tcgagtcccg gctcccgccg aaactgacgc acgtcataaa ctttcgcttc actgccgcga
    15961 aggagacgca cccgcttctg gtcggcaaaa tcgttgaacc cacccgctgc gctgattgcg
    16021 gtcagcaggg tcatatcctc ggtaaaaggg atgcgggcgg gggcgcggac tgcgccgccc
    16081 acgttgacaa agcgtgtctg cgactgcatc acgatcgtga tcgtggggtt ggaataaatc
    16141 ctggcggact tgtagccatt ctcgatcgca ctggcgagct gcgccggggt caagctctgg
    16201 gccctcacct tgttgatgaa aggcagattg accatgccat tggcatccac cgtgtagaca
    16261 ttgttgacct gggtttgctc gtccgccggg acgccgctga tccggatgtc cacggaatcg
    16321 ccggcgcgca ggacggcatc cgcgaaagcg gcgggggcga gcgcggcgag aacaagcaac
    16381 gccagaaacg aggtgcgcat cagtattcct ccgggcgagc cgcgccgttg cgcgcggggg
    16441 ccatcgtttt ctgactgcga gttgctttca gatcgcccgg ccgctgatga tagtaatggg
    16501 catagctcgt gtagtagtag taattctgat cgtgtctcag gtcgacattg ttcaggacca
    16561 cgccaagcac gctgcctccg actcccacga tggcctgttt cacgcgggtc aacatcgcgc
    16621 gcgggaaacg gcgatgctgc acgacgatga tcgtctgatc gacttcactc gcgaggacgg
    16681 aggcgtcgct tgccccgaga atcggtggtg aatcgaagaa gatgatgtcg tagcgggtct
    16741 ttagttcggc gatgaggtcc gacatgcgct gcgaattaag aattccgacc gcatcggccg
    16801 gcagaacgcc gcttgggagg aggaaaagat tcgggaccgt cgtggggaaa atcactttct
    16861 cgatcgccag atcggtcgtc agatagttcg tcaggccaaa tttctcatcg agatcgaaga
    16921 gggtctgttg caccggccgg cgcagatcgg cgtcgacgat caaggtggaa tacccgcctt
    16981 gcgccgagac cagagcgagg ttggccagag tggtcgattt cccctcaccc tgtccgccac
    17041 tggagagcag gatggtattg gcgtcgagac ttttccggtt gaactcgatg ttggtgcgca
    17101 ggatccggta ggcctcggca tcgggcgtat cgccgccttc gcggaaaagg agacggatgt
    17161 tctgcgggat gatggccagg accggtacgt ccagcagggt ttcgacgtcg tccatcgttt
    17221 tcacactcgt atcgagatac tcgatgaaaa atgcgagccc gaggccaagg accagaccga
    17281 cgaggccgcc gagcgccaga ttgagaacga cccgcggacg ggccgcgacc gacggcggct
    17341 cagccacctg cttcacgctt acggccaggc gcggcatcgc catctccatc gtttcggttt
    17401 gggctcgcaa gcggacgttg tcgcgcaggc tcttctcctt gatgtaagcg tttttcgcac
    17461 gggtgtagtt ggcactgaga ttcttggagg cgagttgctg ttcgttgatt gttccgagat
    17521 gtttttgcaa ctcgtcgtgc gtggcttggg ccgaatttag attgcgctgg agcccatcgc
    17581 gaacactcgc cacctgttct tccaattgcc gcacgtaaac cgccttcgtc gcccgcagcg
    17641 ccttgacctt gggatgattc tctcccagtc cggaattcaa gagctgcgcc tcgttggcga
    17701 ccgcttcctg atagctaggc agaatttttt ggatgatcgg atcctggatg ttgagcgtcg
    17761 gcatcatccg catcagatcg tcgcccttga gcgactcgat ttgtttcagc ttggcggcga
    17821 gggtggcgac gttcgtctcg gcgtcattca cttcgccctc ctgtttgatg acgttggagt
    17881 tgtccgcggt gagcgaatct tcggtccctt ccgggttgag atcgatgatg tgttcttcat
    17941 cgcgaatccg cgccacttcc ttgcccgctt tctccacctt ttgctcttct ttcgccacct
    18001 cctcgttcat cgtctcgacc gcgcgattga gaatttcctt ttcctcctgg acgcgcttct
    18061 cctggtaaac ggcgacgatt ttattcgcga tatcagccgc ctcctgcggg ttggtatcgt
    18121 agacgctgat ctggagaagg tcggtattgc gaacttcatc caggtcgagc ttctcacgca
    18181 gcatctgata ggcctgttcg cgcgacgggc gattgccatc ccgggcccat ttatcctgta
    18241 acttcaaatc ctcgatgacc ggataaagaa tgcccgtccg ctgaatgatt tgataaaccg
    18301 tcggcgcaag ctgcgggtcg tgatagggaa tggtcccatc gtttccgaag atccggggag
    18361 tgtcggcggt cgattttact tcgataaaga ccgacgactg atactcgcgg ggctgaaagt
    18421 aagtgacgac cgctgccgtg atgacaacga ggaggaaagc gaggagaatg agcacccaac
    18481 ggacgcggat gacacgccaa taatcgagga aatggagccg gacttcttcg gtgtgtgcca
    18541 tggatttccc ggttcgaacg ggcggtgaat ctaccagaag attgacctga agcaaaaagc
    18601 gcgcccgcta gaaacccacg cttaggccga catagtatcg gttccgcgaa tattcacgaa
    18661 aactgagatc cgatagatcc tcggtacggt cgtagttggc gagcagggtg aacgtccgat
    18721 tgatctcgta gcgaagcccc agactgagat cgatcaaatc ctcgttgaag gccggggcga
    18781 cgaagggcgg cgttgtaacg ctcttgttct gatcgtgctc gtagaaaact gccatcgtgg
    18841 cgctaactcg gggagagatg gcataggcga catttaagcc ggtgcggaag gtcgtgcgcg
    18901 aggcactgcc gggcacgtcg ggttcgtcga ggccgtaatg accgttccag ctgaccgagg
    18961 tccgctcgcc aagcgaatac ttaagcgtcg cctcgccata gggcgaagtc tctgtgccga
    19021 agttatcgaa tgcgcgaaat tcgacgccga agcgctgcga gacgatgaaa tgcggactaa
    19081 agctgtgatc cacgccagcc agaacaaaat gcgtcgtcga gtcgcgcggt gcggtatcga
    19141 aatcaatgat ttcataacgg tactcgccca cgagcgtcgt cgtgggcaga gcaaggtagc
    19201 gaaactcatt tccgaaggta tgttcgaagc gatcctcaaa agcgccgatc gcgctgtttt
    19261 cgtaattgat cacgcttagc gagtaactcg tgacggtcga gaagcgcggc aaccattgga
    19321 aagaggtgga gaacttgtcc gcggtataga agtagtttcc gctccgtcgg ttggtcccga
    19381 cgccggtgtc gaaattcggc tccgactgat acgccagatc aaccgaggcg gcgaaaccaa
    19441 gacgcggggt cgcctggtga ctcatgttca gcgtcaaccc gttattaata tcgtaggtct
    19501 gccctaaagg ccgaaagtaa taataggtgg ccccgccgaa aacctgcagg tcgagattgg
    19561 tgcgcgcgct gccaaattta taatcgaggg ccacgctgcc accggtgaaa aaggacccga
    19621 ttggattctg gtgcgtcgta taaacgttgt cgtcgtaacc ctcctgcacc gagaccgaga
    19681 ggcggagggg cgagggagca aaaattcccg cgccgagatc gagtcctgcc gggccggcct
    19741 cgccgctgcc tgtgctgctg gaaacagcgc cgaatgtctt gccggaactt tcctcctggg
    19801 cataaagagg aagcggcgcg caggtcagcg ccaggaggga aacgatcaac cctgccgccc
    19861 gtggaaaggg ccctggcgtt ccatggaaaa gaagggcatg gaaaaacgcg cgcggatgcg
    19921 acggtctttt tggggccatt gccgcgaagg taggcgttgg gtgaaatcgc ggcaatctct
    19981 tttcggcgac aatgaaatgg gcaatctcac cctcggcacg gagtccaaaa tcagtcaggg
    20041 cgattcgtcg gccggctctg accagggcgc gcgctcttat cccgcaaatt ctatctcggc
    20101 tcagactcca cgccggactt tgttccactc cagcgggcga gatgggcagc gtaggatttc
    20161 tggacgacca tcgatttgga atcccagtcc cgcgctctgc catacatcca ttccgcctcc
    20221 gggaaacgtc ccagcgcatc atagacccgg ccgagcgcga ggaggaaagt ctcatcgtta
    20281 ggcgcaagcg cccgcgccct ctccagcggc tccagcgccg cgcggtaaaa agaatccctt
    20341 gccatcgcgt tcgcgctatc ctccgcccgc cggatgcggc tttcggccag ataaaaaaaa
    20401 atctccgggt tctgcccatc ccgcgcgatg gcctggcgtg cccagcgcat cgcttccagg
    20461 tggcgttcat cccgcagagc ggcccgcgat cgctcggcgt aatcctccgc tcgggtgtat
    20521 cgggctgagc cggcgattaa gaggagagcg agtagcgcca ggccaagacg accggaggtc
    20581 acccgccgaa tggcacccgc accctcgttt tcacggtcga acccggggtt ggccagaatc
    20641 ccaaagacga aggcgagcaa caaaacattg gccgggatgt ggaggttaaa atcaagcaac
    20701 gaatgcaccg ccaaagcggt cacggcgccg agtgcaccaa gatttagcgc caggcggttg
    20761 ctcagcggct gggaagtaag cggcatccgc cgcggcccta gttcttgaaa ggcgatccca
    20821 ccgcgccaga gatgtgcgcc gagaaagatg agaaaggctg ccccgccgac ggcgccatat
    20881 tcggcgagca attgcaggta atcgttgtgc acttcgaccg gatccagttg tacgcgcgag
    20941 gtccgaaacc gtcttccgta gtagagataa gtcccgctcc cggtgccgac ggcgggctgc
    21001 aatttccact gcgcgatcgc cccctgccag agatccagac gaatgttccc ggtatcgaga
    21061 agattctgcg cccggccgct taggtaatcg cttttcttga atccaaaaaa gagcaccgcg
    21121 ctcagcatga gcgcgaaaac gccgcccacg accgcagtgc gccagaaaat ctcgctcccg
    21181 gccttgcgca atgcgaccag tgttagcacg gcgaccacga cgaggcttac gcccgcgctc
    21241 cagtagccgc cgcgacttcc cgtcagcgcg agtccaacgt aacaaagagc gccggcatag
    21301 ccgatcagca atttggtcca gagcggccag cgactccagc aggtgagaga gacgcaaaag
    21361 atgccgagca cctcaagcaa ccccgccagg tgattcgggc agccgtagaa tccgctcgcc
    21421 cgccgcccgt agtcgaagcg ctgcaaaaac gggatcagca tgaaattgtt cccgttgcga
    21481 aattgaatcg caccgatcac cacctgcacc aatgccacca ggatgagaaa caaaagaatg
    21541 gccattcgcc accgagccga agtgaaagtc agcgcgacag cgagataaac gaccagcccg
    21601 cccagcaccg ggaaaatgtc ggcccgcgca aggtagggga caggcgagaa ccaggcacga
    21661 acgagaacat agccaaagaa aaacaccgaa gaccaaagac aggcgcggcc gggcgaaggc
    21721 ttcgcctgcc ggaaggggac gaccgcgaac aaacctccaa gcccgaggag cgcgtagacc
    21781 ggcagggcat aggccaggcg cgtgccgccg atcaagacct gcatcccgac caaagcaatc
    21841 gccactacga tcgcgccaag catctcgctg gcgcgagaga tgagcgatgc gtttccggcc
    21901 acagagcggc gagattacag caattcgcca cgcaccgcca aggcaggatc acccgcttac
    21961 ttgttcgccg tcggactcgg cgtcgggggg cacaagccaa ccacagctcc ctgatgagac
    22021 tggagatatt cgtcgagctg gctttgtcgg accgtacgct gggtgccgtc atcacacacc
    22081 aggatgagct gctcgtgtgg atcaaatccc tcgcccgtgt ctttgctctt cgcatcggac
    22141 aatggattct gattgtccgg cccggccgcg tcggagtcgg cacgctcgcc gccgccgctc
    22201 gccgcgtggg aaaccacgct ttgaatcgcg tccgcgcaat cgggcgccgt cgcgatcgcg
    22261 gcgtcagcga ccgtcgtcgc cgcattgtca tttgccgaga tcgccgcgga cacgatcgac
    22321 ccgacaaatt tacaatcgcc tttcgcgccg cagcgcagca cggttccgac aatttcgccg
    22381 gtgaactcgc ggcgcgtccc gaccgccacg tccgtgatgg ccgcggccac cgtccgatgt
    22441 ttccgcacag cggcgcagag ggccgcgaga aattccgact tggtcgcccc cttgatcgtt
    22501 ttcgcggctg gcaaaccgcc ttcaatcagc tccttcggcg tccggtcgcg accgagtgcc
    22561 gcggccgggg cggcgaccca aagtccggcg gccagggcga ccaccaacaa gaacaagaag
    22621 gcgctccacc cgatcctcgc cgggactggc ccgacgcttg aaaccgcggg tattttttcc
    22681 cgcgacaggc gaggatgagt tcggcttctc aaagcgacga tgatcactat tttgcgcgct
    22741 cgtcagaaag cgggcaacgg gaatcgaacc cgcatggcca gcttggaagg ctggaacttt
    22801 accattaagc tatgcccgcg cgggatgaag ctgccgcgcc cgcacctgct tgccaagtta
    22861 tttccgagaa tgtggggggc ctttgcgccg cgatcgtcgc cgcacaaagg cggctcccac
    22921 tttccccaaa cttgccaatc gcgccgactc cagctagcaa ctgccatatg aaatcactgc
    22981 atctgttcgg gatcgcttct ctcgcgcttc ttgccaccag ccgtgccgac ctgaccatcg
    23041 tccagaaagt ggatggcgcc gggggcggcg tccacgagct cactctgaaa gtgaaaggcg
    23101 acaaggcacg cgtcgatatc aacccgcaga tcactactat catcgacgcg aagaccggcg
    23161 aagtaacgac ccttttgaac gataagaaaa ccgtgatgcg catctccggt gagaaggcca
    23221 aagccatggc cgagatggcc aagtcatttg ccaaggaaga gtcacccacc caggccttgc
    23281 ccaaatccac cggcaagaaa gaaacgatca acggctacga gactgaagag tatgtcaccg
    23341 attcgccaaa gtaccacgct agctactggg tcgcgaagag ctacccgaat tatcaaagta
    23401 tcctccagca gatgtcgatc atgcaacaag gcgcctttgc cgggctgacc aaaggcctgc
    23461 cggattacca cgcccttccc gggcttccgc tgcgcactca ggtcaggatt cccgggcagg
    23521 acgatgtcac gagcacgatc gaaagcctca aactggatcc cctccccgat tcggactttt
    23581 ccatcccggc tggctattct gaaatcaaga tgcccgattt catcggcggc aagcaaccgc
    23641 ccggcaaacc ggacgatccc ggcaacgaat gaccgacgac gcgccgcgtc tcatcctcgc
    23701 ctcggcctcg ccccgccgcc gccagttgct gactgaggcc ggcttcgctt tcgacatcgt
    23761 cgcgcccttg gtggacgaaa atctctctga ccatttcacg ctccgtgagg tgacctgctg
    23821 gaatgcgctg cgcaagggcc tcgccgtggc acgggctcat cccgagcagg tggtgctcgc
    23881 cgctgacacg ctagtcggcc tcggccacca aatcatcggc aagcccgccg atcgcgccgc
    23941 ggcagtgcgc gttctgcgtc ggctcagtgg ccggacgcac atcgtcgtct cggcagtttt
    24001 catcgcgcat ttgcgtcgcg gaaaatcgga aagcttcgcc gacacctcgc gcgtggtttt
    24061 caaaagattg agcgaccgga tgatcgcgga ttatctcgcc gtgatcgacc cgctcgacaa
    24121 agcgggcgcc tacgccgcgc agggccacgg gagccagatc atcgcgcgaa tcattggttc
    24181 gcggagcaac gtcatcggcc tgccattgga gaagacccgc gccgcactcg cgcgcttcga
    24241 tcttcaccgg cggtcggcct aacgcctaac gactttccac cggcgtgcgc tcatcgtccg
    24301 ccggcaccgc gagttcctcg cacttttcgc ggcgcaggac gcggtaaagg aaccaaacga
    24361 tgaaacaatt agccgccacg agaataagcg cgaccgcgcc cggactgcgc caggcgtgcc
    24421 gcacttcgaa gggaatcaac gcgccagtcg ccaccaccat gagcacctcg gcccagcgct
    24481 tttgcagata aaccccaatc ccctcagtgc aaaggacggc cgccgagacc agcgcgacta
    24541 ggccggtcac ctggagcaag ccgcccgcga ccacatcctg ccacttgctc aggagataaa
    24601 gcatcgcacg gctgtgcgcg accatcagct ctccatcgac ccactccgag atcccattca
    24661 gccagagcgt gcgactgtgt aaaaagagca gggaaatccc gatactgaaa agcaccgcgc
    24721 cctgcagaat cttgaaaatc gcgatgattt tcagataacg aacgcggcgc tgggggcgca
    24781 gcatttttgg gatcggcgcg acgtcgctca ttccgatacg tctcgcagaa gcaaacgcga
    24841 tctcaagcgg cgcggctctg ccggcagcag atctcgagct tgttgccgct cgggtcctcg
    24901 aaaaaaaacg cgtagtagcc gggcgagtat tcgcgccaga gctccgggcc ttcgaggttc
    24961 tggccgcccg cttgcctaac gatctcggcc aggcgatcca cttccgcctg gctctccgcc
    25021 cagaaggcga tccggttatc gttaggctgg tgtcgcggcg cttcggtaaa accgaaaaat
    25081 tccaccggac cgtcgcccgg tgcctgccag atggaccact cgccgctgct cgaagcgatc
    25141 gtaaaaccga gctcgggcag gatccgggcg taaaacggac gggccgccgc gatttctcgc
    25201 acgcgcaggt caatgtggtc aaaccgtcgt gttttcatcg tcgttaggca ggttgcagga
    25261 gccagcgcgc gatcgaaaga taaaagagca aagttaaaag gtccgccagc gcgagcgtga
    25321 gcggcccggc ggcgattttt ggatccagcc gcagggcgtg caggagcgac ggaatgctca
    25381 ggcccaggag gcacgccatc acgagcgagc tgagaatgct gcttccgatc acgagtgcgg
    25441 gccagccctc gcccagccag aaccagacaa tcagtgcgac gagcaaaccg caggccgcgc
    25501 ctaacaagag cgaaaccgcg acttcgcgcc gcagcgcccc gagataccag cgcggggtgg
    25561 gccgggcaac gcgcagcgcc tggatggtca ccgtcatcga ctgcatgctg acgctttcgc
    25621 caagcccgag cacgagggtg aggaaaaacg cgatcaccag gctctgggcg agcgtgagct
    25681 gaaaggcgct cgcgagcagg gcgcaggccg tcccgcccac aatcgtggcc agcagccacg
    25741 gaaagcgata gcgaaacgcc cgtagcggcc ccgcatcgcg cacttgcgcc acgtaaaagc
    25801 cgatcgtctc aaaaacatcc tcgtgctgcg cgcgctccgc gatgtcgaag acttcctcgg
    25861 taaagacgcc gacgtcgatc aagccgagga tgcgtcgttc cgtgtccacc accgggaagg
    25921 caaaaaactt atggagcacg aaaaattcgc acgcttcgag cagggtcgcg gtctgcggaa
    25981 tggcgatgat ccgtttgatc atcaaatcgc cgatcctttg atcgagcggg gcggtcagca
    26041 agcgccgggt cggcagcacg ccgacgagcc ggttctcctc gtccacgaca tagaaataaa
    26101 tgatcttttc gccgatccct tcctcccgca cggcgtccag cgcctggcgc gcggtcatcg
    26161 attcctggag ctgcgcaaaa tccttccgga cgaaatcgcc gaccggccgg tcgagatgtt
    26221 gcggcgcggc gttcatcggc cggatgataa ctgctaagcc gccatcgttc gagacacgaa
    26281 gtagatcgtc cttgcctgac ggtcgggcgc cggaaacact cgggccacct atgactcgcc
    26341 ggataaaacg aatcgcgccg ctccaagccg gaaaaatgct cgccgtggtt tacggctgca
    26401 tgggtttgat tttcctcccc ttcttcgccc tcgccgggac gctcggcgct ttcgcgcaac
    26461 atgcccaacc aaatcaggcg gcgccggcgg cgctcgttgg tggtctcctc gttgggatgg
    26521 gatttttctt cccaatcatc tacgccgcga tgggattcat tttcggcgtc gtcggcgcgg
    26581 cgctctacaa tctcgttgct cgctggatcg gcggcttcga ggtagaggtg gaatagcaga
    26641 agagaccaca ttttctcgac gtctgttccg cgcg
//
LOCUS       DATMNS010000271         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3482195, whole genome shotgun sequence.
ACCESSION   DATMNS010000271 DATMNS010000000
VERSION     DATMNS010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3482195"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..650
                     /locus_tag="VNW28_09570"
     CDS             138..650
                     /locus_tag="VNW28_09570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXA10220.1"
                     /translation="MTPSRFTIRLAGVSDAALIATQRARMFQEMDLVPEPLFAIYRAQ
                     CESRLRAMLESGEYLGWLAWPNESPGQIVAGVGAQLRSVLPHPVEGPGGEIGLAEGRH
                     AVVINVFTEPEWRRRGIAKLLLEHLIAWARNERLDRLVLHASDEGRALYEKMGFVQTN
                     EMRFKDRLDR"
     gene            760..1923
                     /locus_tag="VNW28_09575"
     CDS             760..1923
                     /locus_tag="VNW28_09575"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXA10221.1"
                     /translation="MERIHLNRTSFQTAFVLILVLAVSFAFLAVAWPFLIPLLLGALL
                     AGLCDPLYRWVTRLLGGRRSLAAGLTLLILFVLIVGPLSAFVGVVVRQALAVSNQALP
                     WLQEHFGEVSAFNAHDWLARRFPALAGYVPKQAQIVDSTAQLAKATGTYLVGGATQLT
                     ASTATFLLNLFVMLYAMFYFLRDGGDILRKILYYTPLSHGDEVRVLERLRSVTRATLK
                     GTLVIGAIQGTLAGIGLRFAGLGSAAFWGTIMAVLSIVPGIGAALVWGPAVIYLFIIG
                     QKLTATLLLIWCSAVVGTIDNMLRPRLVGKDAKMPDLLILIGTLGGLFLFGLVGFIIG
                     PLICGLFLTVWDIYGATFKDVLPPVGELGPSQMKIADPDLTARDKSESQSGLK"
BASE COUNT          359 a          651 c          601 g          448 t
ORIGIN      
        1 ggattcactt gtcccgaatg gtcgcatctt tccgccggag attccgtcga atttagccga
       61 aggctcgtcc cgcacctccg caccgcgctc gacgcgggat cggttgcgtc cgctcgttaa
      121 cgaccgaagt ctgctgcatg acgccgtcgc gtttcacgat tcgcctcgcc ggcgtttccg
      181 atgccgcact catcgcaacc cagcgcgccc ggatgttcca ggaaatggac ctcgtgcctg
      241 agccgctctt tgcgatttat cgcgcgcagt gtgaatcccg acttcgcgcg atgctcgaga
      301 gtggcgaata cctcggttgg ctcgcctggc ctaacgaatc gcccggccag atcgtcgccg
      361 gtgttggcgc gcaacttcgg agcgtcctgc cgcatccggt cgaaggaccc ggcggagaga
      421 tcggcctcgc cgagggtcgc catgccgtcg tcatcaacgt cttcaccgaa ccggaatggc
      481 gccgccgcgg gatcgcaaaa cttttgctcg aacacctcat cgcctgggcc cggaacgagc
      541 gcctcgaccg cctcgtcctg catgcctccg acgaaggtcg cgccctctat gaaaaaatgg
      601 gcttcgtcca gacgaacgaa atgcgcttca aggatcggct cgaccgttga gaaaaattcg
      661 gaaatcgtta cgcgcaatca ctcggtcgag ggtgaaaacc acgctcccgg gagcgcaata
      721 ggttgatcct cgggccaccg ccggctcata atgtccgtca tggagcgcat ccacctaaac
      781 cgcaccagct ttcagaccgc tttcgtcctc atcctcgtgc tcgcggtttc gtttgcgttc
      841 ctcgcggtgg cgtggccgtt tctgattcct ctgctcctcg gcgccctgct cgcggggttg
      901 tgcgacccgc tttaccggtg ggtcacacgg ttgctcggcg gacgcagatc gctcgcggcc
      961 gggctgacgc ttctgattct ttttgttcta atcgtcgggc ctctgagcgc ctttgtcgga
     1021 gtggtcgtca ggcaggcgtt ggccgtaagc aaccaggccc tgccctggtt acaggagcat
     1081 ttcggggagg tgagcgcttt caacgcacac gactggctgg cccggcgttt tccagcgctc
     1141 gcgggttacg ttccaaagca ggcccagatc gtcgacagca cggcgcagct ggccaaagcg
     1201 accggcacct atctcgtcgg gggcgcgacc caactcaccg cgagcaccgc cacttttctg
     1261 ctcaacctct tcgtcatgct ctacgccatg ttctatttcc tgcgcgacgg cggcgacatt
     1321 ttgcggaaaa ttttatacta cacaccactc agccatggag atgaggtgcg agtcttggaa
     1381 aggctccgtt ccgtcacccg cgcgacgctc aagggcacgc tcgtcatcgg ggccatccag
     1441 gggacgctgg ccgggatcgg ccttcggttt gcgggcctcg ggagcgcggc cttttgggga
     1501 acgatcatgg ccgtgctctc catcgtgccg ggaatcggtg cggctctcgt ctgggggcct
     1561 gccgtgattt atcttttcat tattggccag aagctcaccg ccactctgct tctgatctgg
     1621 tgctccgccg tcgtcgggac gatcgataac atgttgcgcc caaggctcgt cggaaaggat
     1681 gcaaaaatgc ccgaccttct catccttatc gggacgctcg gcgggctctt cctttttggc
     1741 ctggttggat tcatcatcgg accgctgatc tgcgggctgt tcctgaccgt ttgggatatc
     1801 tacggcgcaa ctttcaagga cgtattgccg ccggtgggcg agctaggtcc cagccagatg
     1861 aaaatcgctg acccagattt gacggccaga gataaaagcg aaagccaaag cggcctgaaa
     1921 tgaaagagtc gttccggcga agtgtctttt ccggaagact gcgccgataa tgcactgata
     1981 tgtcgcggat cgattttatc ggcatcggcg caccaaaatg cgggaccacc tggctttcgg
     2041 cgcagctcga agcccatcc
//
LOCUS       DATMNS010000272         3400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4515796, whole genome shotgun sequence.
ACCESSION   DATMNS010000272 DATMNS010000000
VERSION     DATMNS010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3400
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4515796"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VNW28_09580"
     CDS             complement(<1..327)
                     /locus_tag="VNW28_09580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXA10222.1"
                     /translation="MPPKDHITAAGFRLAESKETGFTLVEVLVALVICVFFGAAAFMT
                     NSQLLAGLKSQRETAAATMLLQRRMEDLRANAWSDIATASFLQASIISNPAPNNPNLT
                     QQQIAAY"
     gene            complement(454..1350)
                     /gene="rfbA"
                     /locus_tag="VNW28_09585"
     CDS             complement(454..1350)
                     /gene="rfbA"
                     /locus_tag="VNW28_09585"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HXA10223.1"
                     /translation="MNKKAILLAGGAGTRLYPLTKIVCKQLLPVYDKPMICYPLATLM
                     LGGLREILVISTPKDVPMLRDFLGDGSQLGIRLEYAEQAEPKGIAQAFLIGASFVGAA
                     GAALILGDNIFYGKLDFFRNALALESGACIFGYQVRDPERYGVVEFGPDGRAISLEEK
                     PSKPKSHYAVPGLYVYDGQVVEFCQSLRPSARGELEITDLNLIYLRQNSLHVRLLGRG
                     MAWLDTGTQTSLLEAANYIATIEHRQGLKVACLEEVALRAGFIDQAQLGRLIDRLPKG
                     EYRDYLRLVAEEETTQAAADSV"
     gene            complement(1343..3277)
                     /locus_tag="VNW28_09590"
     CDS             complement(1343..3277)
                     /locus_tag="VNW28_09590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXA10224.1"
                     /translation="MQLAYLYSRYPVVSQTFCDMEMLELERRGYDLLIGSVHPPLTSL
                     RHEHFTRFRSTVHYAPPAPIMRLWEQKTRAAERWPESLIERHDRQYGASFKAALRARN
                     ASYFAELFTRHGINHFHVHFANRAAHTALFVKEISGIPFSITAHGQDFMSDLGQDNLL
                     REICSAAEFVAVETDFSRDLLRERCPDAAAKIHRVYNGLALAELPASLRGERSPGPTR
                     ILSIGRLVPFKGFEILLEACAELDRRNFDFRCEIVGDGPLREKLEAMIVRLKLARCVE
                     LCGSLSQADVFSKLRSCDIFALASVVDSEGASDVFPTVIMEAMAFARPVVSTRLAGIP
                     ESVVEGVTGLLVAPGDWEEFALALDRLVRDPVRRKRLGDAGRARMENEFDVVKTVEPL
                     HQLFSACISASSVQRPKPAPPGVELKQTAYLIDRWPDESLPFLEMELRALRRNGVPHV
                     PFVFCPPLSARLTAKTKDLAMEFQYLPDAMVIEAEWQSSMPLVRELEAMRANQKHRPP
                     SDLFLEQARYAVMLRKLFLQHNVGHVHATNSRSLLCALCLKKLLGLGISVAIEEKPVL
                     SEAVILDALDQCVGGRSIDRELLAHRGSGFLFDQTLERPSVNDIGPWLTRKARVEWTG
                     ARRFWREWSQQLIGWTAAHE"
BASE COUNT          739 a         1017 c          992 g          652 t
ORIGIN      
        1 ataggcggcg atttgctgct gggtgaggtt tgggttattc ggagccgggt tggagatgat
       61 ggaggcttga agaaatgagg ccgtcgcgat gtcgctccat gcgttggccc gtaaatcctc
      121 catgcgacgc tgaagcagca tcgtcgcggc agcggtctcc ctttgcgatt tcagccccgc
      181 gagcagttgg gagttcgtca tgaaagccgc tgctccgaag aaaacgcaga ttacgagtgc
      241 cacgagcacc tcgaccaagg tgaagccagt ctccttcgac tcggcgagcc taaatccggc
      301 tgctgtgata tgatctttgg gaggcacggg caggataaaa aggagcaatg aacatgccgt
      361 atgttataat ttccataatg cacgacgcca gtctctggcg gtataaggaa agagctgttc
      421 ggtcccccta gaagaagaac ccagactcgc ccgctacaca gaatcggccg ctgcctgcgt
      481 cgtctcttcc tctgcgacca ggcgaaggta gtctcgatat tcgcctttcg gcaatcggtc
      541 gatgagccga cccagctgcg cttgatcgat gaagcccgcc cttagggcaa cctcttcaag
      601 acaggcgacc tttaatccct gccggtgctc aatcgtcgcg atgtaattgg ctgcctcgag
      661 cagactcgtt tgtgtgccag tgtcgagcca cgccatccct cgaccgagaa gtcggacatg
      721 gagtgaattt tggcggaggt aaatcaaatt gagatcggtt atttccagct ctccgcgcgc
      781 ggagggtcga aggctttggc agaattcgac cacctgccca tcgtaaacgt agaggcccgg
      841 gacggcgtag tggctttttg gcttgctcgg tttctcctcc aggctgatcg cgcggccgtc
      901 gggcccaaac tcgaccactc cgtagcgctc tgggtcccgc acctgataac cgaaaatgca
      961 ggcgccgctc tcgagcgcga gcgcgttgcg aaagaaatcg agtttcccgt aaaatatatt
     1021 gtcgccgagg atcaatgcgg ctccagccgc gccgacaaag ctcgcgccga taagaaacgc
     1081 ctgtgcgatc cctttcggtt ccgcttgttc tgcgtactcg agacggatcc ccagctgcga
     1141 gccatcgccg aggaagtcgc gcagcatcgg gacatccttc ggcgtggaga taacgagaat
     1201 ctcgcgcagg ccgccgagca tcagcgtggc cagcgggtaa caaatcattg gtttgtcgta
     1261 gaccggcaag agttgcttgc agacgatctt ggtcagcgga taaagccgcg tgccggcgcc
     1321 gccggcgagg agaatcgctt tcttattcat gcgcggcggt ccacccgatc agttgttgcg
     1381 accactcccg ccagaagcgg cgcgcaccgg tccattcgac cctcgctttc ctcgttaacc
     1441 aagggccgat atcgttcacc gacggccttt cgagcgtctg atcaaacaag aaaccgctcc
     1501 cccgatgcgc cagcaattcc cggtcgatgc tgcgaccgcc gacgcattgg tccagcgcat
     1561 ccagaatgac cgcctcggag aggaccggct tttcctcgat cgccacgctg atccccaagc
     1621 cgagcagctt cttcaagcag agcgcgcata acaatgagcg cgaattggtg gcatgcacgt
     1681 gaccgacgtt gtgctgcagg aataatttcc gcaacatcac agcgtagcgc gcctgctcga
     1741 gaaacaggtc ggagggtggg cgatgctttt ggtttgcgcg catcgcttcc agctcccgca
     1801 ccagaggcat gctggattgc cattccgcct cgatgaccat cgcgtccggc aggtattgga
     1861 actccatggc aagatccttg gtcttcgccg ttagtcgcgc ggacagcggc ggacagaaaa
     1921 cgaaaggcac gtgaggcacc ccgttccggc gcaaggcgcg cagctccatc tcgaggaacg
     1981 gaagactctc atccggccat cggtcgatca gatacgccgt ttgcttaagc tccacgccgg
     2041 gcggggctgg cttcggtcgc tgcacggacg aagcggaaat gcaggccgag aaaagttggt
     2101 gcagcggttc aactgtcttc accacatcaa actcgttttc catccgggcg cgcccggcat
     2161 cgcccaatcg cttccgccgc accggatcgc ggaccaacct atcgagcgca agagcaaatt
     2221 cttcccaatc acccggcgca accagcaggc ccgtgactcc ttcgacgact gattcgggga
     2281 ttcccgccag ccgggtggag acgaccggcc gggcaaaagc cattgcctcc atgatgaccg
     2341 tcgggaaaac gtcactggcg ccttcggaat cgacgacgct cgctaacgca aaaatgtcgc
     2401 aactgcgcag tttcgagaag acatcggcct gggaaagtga gccgcaaagc tcgacacacc
     2461 gggccagttt caaacggacg atcatcgcct cgagcttttc ccggagcgga ccgtcaccga
     2521 cgatctcaca acgaaaatcg aagttccgcc ggtccagctc ggcgcaggct tcgagcagga
     2581 tctcaaagcc cttgaacgga acgaggcgac cgatactgag aatcctggtt ggaccaggcg
     2641 agcgttctcc ccgcagagaa gccggcaact cagcgagtgc aagaccattg tagactcgat
     2701 ggattttggc cgctgcgtcg gggcatcgtt cccggagcag gtcgcggctg aaatccgttt
     2761 cgaccgcgac gaattccgcc gcgctacata tttctcgcag cagattatcc tgtcccaaat
     2821 ccgacatgaa atcctggccg tgcgcggtga tgctgaaagg gatgcccgag atttccttca
     2881 cgaaaagtgc ggtatgcgcg gcgcgattcg caaaatggac gtgaaaatga ttgatcccgt
     2941 gacgggtgaa aagctcggca aagtaggagg catttcgggc tcgcaacgcc gccttgaagc
     3001 tcgctccgta ttgacggtca tgacgctcaa tcaagctctc cggccagcgt tccgcagcgc
     3061 gcgttttctg ttcccagaga cgcatgatcg gcgcgggcgg cgcataatga acagtcgacc
     3121 gaaagcgggt gaaatgttcg tgccgcaggc tggtgagggg tggatgcacc gaaccgatta
     3181 acaggtcgta accgcgccgc tccaattcca gcatttccat gtcgcagaag gtctgcgaaa
     3241 ccactggata acgagagtaa agataagcga gctgcatcgg acaaagaccg aaacgtcgag
     3301 accgggataa cagagagcag caggaacaaa gccccgccgc gcgctcagtc gaccgcccgg
     3361 cgcggcgagg aatcgctcgt ccggccctgc tttccgcttt
//
LOCUS       DATMNS010000273         2838 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4152816, whole genome shotgun sequence.
ACCESSION   DATMNS010000273 DATMNS010000000
VERSION     DATMNS010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2838
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4152816"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VNW28_09595"
     CDS             <1..1410
                     /locus_tag="VNW28_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HXA10225.1"
                     /translation="RSAPQPSGPPTSATSSNAGPTEFRLPELGENISQGDLVRLMIAP
                     GTKVSEGQPVMELETDKAVIEVPSSISGVVKELRVKQGEKIKVGQVIFTIAGTAAPQP
                     ARPTHVPVEHVSGQHGARLAFQAAIRAEGKTEEQALPPDQPQQAAPVFSMPVQLGKVA
                     GTEHRQPVPAAPHVRRLAREIGIDIYEVKGGGPGGRISEDDVKAYAKALVSAAATAVQ
                     APRAGHFTQPKLPDFSKWGKVEHVSMRGVRRKTAEHLAEAWNTIPHVTQHDRADITEL
                     EQLRARFAPKAQEAGGKMTVTAIALKVCAAALKVFPQFNASIDVEKEEILYKQYINIG
                     VAADTDRGLLVPVIRDVDKKNIVELAVELSQFSKKAREKKLTPADMEGGTFTITNLGG
                     IGGVGFTPIVNFPEVAILGLSRSRMEPEWINGKFEPRLILPLSLSYDHRLIDGADAAR
                     FLRWIAEAFEQPFLLSVQG"
     gene            1510..>2838
                     /gene="lpdA"
                     /locus_tag="VNW28_09600"
     CDS             1510..>2838
                     /gene="lpdA"
                     /locus_tag="VNW28_09600"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXA10226.1"
                     /translation="MTETLNIAIVGAGPGGYAAAFLAADLGMTVTLIDPEVNPGGVCL
                     YRGCIPSKALLHIAKLIEESEQAKNWGIEFGAPKIDLARLRGFKENVVKKLTGGLGIL
                     SKQRKVRYVQGRAAFENSTTLSVTKSDGSTESLTFDRIIIATGSRPAIVPTLKLDTPR
                     MMDSTGALNLEDIPGTLLVVGGGYIGLELGSVYAALGTRVTVVEMLPGLLPGADRDLV
                     LPLHKRLEKLFDGILLNTTVASVKDEGPAIRATLKAQDGTTQEKVFDRVLVSVGRKPN
                     SEISGLEKTRAQVGQRGFIQVNKQLQTDDPSIYAIGDVVGEPMLAHKAMHEGRTAVEA
                     IAGHKVAFEPAAIPAVVFTDPEVAWAGLTETQAQNENREIKIAKFPWAASGRAVAIDR
                     TDGLTKLLIDPQTERVLGVGIVGAGAGEMIAEGVLAIEMAALAHDVAATIH"
BASE COUNT          624 a          937 c          803 g          474 t
ORIGIN      
        1 cgatccgcgc ctcagccttc aggaccacca acctcagcaa cctcatcgaa cgccggtccg
       61 accgaattca ggctgcccga actcggagaa aacatctcgc aaggcgatct ggtgcgcctc
      121 atgattgccc cgggcacaaa agtctccgaa ggccagccag tgatggaact tgagaccgac
      181 aaggcggtga tcgaagttcc ttcgtcgatc agcggagtcg tcaaggagct tcgggtcaag
      241 caaggcgaga agattaaagt cggccaggtc atcttcacga ttgccggcac ggccgctcct
      301 caaccggccc ggcccacgca tgtcccggtc gaacacgtct ccggacagca cggcgcgcgc
      361 ctcgcatttc aggcggcaat tcgcgcggaa ggaaagactg aggagcaggc gcttcctccc
      421 gaccaaccac agcaggccgc accggttttc agcatgcccg tacaactcgg caaagttgcg
      481 ggcacagaac atcgacagcc cgttcccgcc gctccgcatg tgcggcgtct cgcgcgcgaa
      541 atcggcattg acatctacga ggtcaaaggc ggcggccccg gcggacgcat cagcgaagat
      601 gacgtcaaag cctacgccaa ggccctcgta tcggccgcgg caaccgcagt gcaggcgccg
      661 cgcgcgggtc atttcaccca gccgaaactt cccgacttct ccaagtgggg caaggtagag
      721 cacgtctcca tgcgcggcgt ccggcgcaag accgcggagc atcttgccga agcctggaac
      781 acgattcctc atgtcacgca gcacgatcgt gccgacatca ccgaacttga gcaactgcgc
      841 gcccggtttg caccgaaagc gcaggaggcc ggcggcaaga tgaccgtcac cgcgatcgcg
      901 ctgaaagttt gcgccgcggc gctgaaagtt ttcccccagt tcaatgcctc gatcgacgtt
      961 gaaaaagaag aaattctcta caagcagtac atcaacatcg gagtcgcagc cgacaccgat
     1021 cgcggcctcc tcgttcctgt gatccgcgac gtggacaaaa agaacatagt cgaactggcg
     1081 gtcgagttgt cgcagttttc gaagaaagcc cgcgagaaga agctcacccc cgccgacatg
     1141 gaaggcggca cgttcaccat cacgaatctg ggcggcatcg gcggggtcgg atttacgccg
     1201 atcgtgaatt ttccagaggt cgcgatcctc ggcctgtctc gctcgcggat ggaaccggag
     1261 tggattaacg gcaagtttga gccgcgcctg attctgccgc tttcgctttc ctacgaccac
     1321 cgcctgatcg acggcgccga cgcggcccgc ttcctgcgct ggatcgcgga agcgttcgag
     1381 cagccattcc tgttgtcggt gcagggatga aaagagaatc atgcaccaca ggggacacag
     1441 aggaacacag cgagatcgaa aagcttgggt ttcccctgtg tacctctgtg acctctgtgg
     1501 ttaaacccca tgactgaaac tctgaacatc gcaatagtcg gcgccggccc cggaggctac
     1561 gccgccgcat ttctcgccgc cgacctcggg atgaccgtca cgctcatcga cccggaagtg
     1621 aatcccggtg gcgtgtgcct ttaccgtgga tgcattccgt cgaaggcgct cctgcatatc
     1681 gcgaaattga tcgaagaatc ggagcaggca aagaactggg gaatcgaatt cggcgcaccg
     1741 aaaatcgacc tcgcccgcct gcgcggcttc aaagaaaatg tcgtcaagaa actcaccggg
     1801 ggcctcggca tactgtcgaa gcaacgcaag gtgcgctacg tgcaaggccg cgcagcattt
     1861 gagaactcga cgacgctgag cgtcacgaaa tccgatggct ccaccgaatc tctcactttc
     1921 gatcgcatca ttatcgcgac gggctcgcgc cccgcgatcg ttccgacact gaaactcgac
     1981 acgccgcgca tgatggattc caccggcgcg ctcaacctcg aagacattcc cggcacgctg
     2041 ctcgtcgtcg gcggcggata catcggcctc gaactcggct ccgtctacgc ggccctcggc
     2101 acgcgcgtca cggtcgtcga aatgcttccc ggccttcttc ccggcgccga tcgcgatctc
     2161 gtccttccgc tgcacaagcg cctcgaaaaa ttattcgacg gaatcctgct caacaccacc
     2221 gtcgcctccg tcaaagacga aggccccgcc atacgcgcaa cattgaaagc gcaagatggc
     2281 accacgcagg aaaaagtttt cgaccgcgtg ctcgtctccg tcggccgcaa gccgaactcc
     2341 gaaatttccg gcctcgaaaa aacgcgcgcg caagtcggcc agcgcgggtt cattcaagtc
     2401 aacaagcaac tccagactga cgacccgtcc atctacgcca tcggcgacgt agtgggcgaa
     2461 cccatgctcg cacacaaagc gatgcacgaa ggccgcaccg ccgtcgaagc cattgccgga
     2521 cacaaagtcg ccttcgaacc ggccgccatc cctgcagttg tcttcaccga tcccgaagtc
     2581 gcctgggcag ggctgaccga gacgcaggcg caaaacgaaa atcgcgagat caaaatcgca
     2641 aagtttccct gggccgcgtc gggccgcgcc gtggctattg accgcaccga cggcctcacc
     2701 aagctgttga tcgatccgca gaccgaacgc gtgctcggag tcggcattgt cggcgccgga
     2761 gcgggcgaga tgatcgccga aggcgtgctc gccatcgaga tggccgcgct cgcgcacgac
     2821 gtcgccgcga ccattcac
//
LOCUS       DATMNS010000274         8672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2065546, whole genome shotgun sequence.
ACCESSION   DATMNS010000274 DATMNS010000000
VERSION     DATMNS010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8672
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2065546"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VNW28_09605"
     CDS             complement(<1..257)
                     /locus_tag="VNW28_09605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10227.1"
                     /translation="MTDALYHDTNITFLRADSGYYQILAHSSPAKQKQNIRRFWRQSV
                     AGHYTPLAFSAEFFFTKLAGTRAAVWRCRQIGAVALVGAAL"
     gene            451..1143
                     /locus_tag="VNW28_09610"
     CDS             451..1143
                     /locus_tag="VNW28_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10228.1"
                     /translation="MRQRFAILFLILAAATAQAAEREYIVLVGGPSLHIWEQYKGPVA
                     HDHWWANFVHSARVRTEQLRAQLGPDAMITWLVYKPGYVDRGVQEKQDLIANINSVRD
                     KFHLHLVYFDRGGDVINYLNEGQPRNQIKVSGFEFFGHSNKACFLFDYSNVVDSASKA
                     WLHETELKKIDRRDFTRDAYVKSWGCHTGESMSKLWREATGTRMWGAIGRTDYSTGEL
                     PALSSVGGRWVN"
     gene            1333..1899
                     /locus_tag="VNW28_09615"
     CDS             1333..1899
                     /locus_tag="VNW28_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXA10229.1"
                     /translation="MAAPAASIYGRSVAALDFEKLVEDFYMPLYRFAFSLSRQESDAA
                     DLTQQTFFLWASKGHQLRDASKVKTWLFTSLYREFLGRKRQQDRFVESENNTEAIAAQ
                     TVPASVVEQLDGDIVQRALLALEEIYRAPLTLFYLQQHSYKEIAEILKVPIGTVMSRI
                     SRGKERLRQALADSAGLTKENRGGNTHG"
     gene            1892..2635
                     /locus_tag="VNW28_09620"
     CDS             1892..2635
                     /locus_tag="VNW28_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10230.1"
                     /translation="MDEQEARLILQAYRLGEADKDDPHFDEALQEAARNPALARWFAE
                     EQAFDRALAAHLDSMPAPFGLKTRILAQAAPPAMGGSSRWIVSLAGVVALLFLLTQVV
                     SLWRGSAPAPALPEYAAEMVSFVQLPPPLDMESQDLGAIKHWLAQKDALPPDVPPQLA
                     ALQPVGCRVLSFRGHEVTLICFKRGGNQLAHLFVVDRAAFPEVKPGTAPVFASEGEWT
                     TASWMEKDRVYTIAVQGDANAVQRYLPHA"
     gene            complement(2709..3152)
                     /locus_tag="VNW28_09625"
     CDS             complement(2709..3152)
                     /locus_tag="VNW28_09625"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXA10231.1"
                     /translation="MRLELNPNEGNIGFQIAPMIDVVFVIMLFFMVMAGAVKIENELN
                     TQLPGTAETSSSTDFVDEQIIAISDTGEILLNDEPFDSAASKDLPQLKGTLLRLKQNA
                     DAARTRAVVTIISDRQAKYSRTIDVLDALALAKIDNVTFTVNEEE"
     gene            complement(3361..3822)
                     /locus_tag="VNW28_09630"
     CDS             complement(3361..3822)
                     /locus_tag="VNW28_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXA10232.1"
                     /translation="MAGGGGGSEAGEPEFQVAPMIDVLLTILVFFMTITSAQVLKVDK
                     SIQLPIAKDAQKKDNSRAEAILNVRWKLPAKKAQFVFDDRTFQAATEMVEPLKNARIT
                     GEKKVTAGANPTFRAVIRGDRDVPAIYVSQAMNACAEAGITDISFSAVNKE"
     gene            complement(3836..4702)
                     /locus_tag="VNW28_09635"
     CDS             complement(3836..4702)
                     /locus_tag="VNW28_09635"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXA10233.1"
                     /translation="MKNMIRTILRLTTRQRTARAFAAALAGAFLLLAAPGFAQDASPA
                     GAAGVHSKTLWEQIKEGGWVMFPIALCSIATLYLIGDGIIRTSRKKTAPPEQEEALKA
                     LFRQGDYIGAHDYCKTNPSPLSNVLRSGIGLLGEGKQVAEDGMIGVLAKENSQMQTYI
                     SYLSVIGVTTPMIGLLGTVTGMIKAFAKLGSSGIGDPSGLSAAIGEVLVATASGLIIA
                     IPAFAAFYFLRNRATDSLHHIQDVVNRLFRKMPYESLAGVHLGDDELFAATPNWLVPD
                     APPERNPTNQLA"
     gene            complement(4699..5523)
                     /locus_tag="VNW28_09640"
     CDS             complement(4699..5523)
                     /locus_tag="VNW28_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA10234.1"
                     /translation="MKTLRFTVPFILLLAVVRPIHAQNILLKTGQTIETKGVRRAGDM
                     VMAKIQVGASSGEVGYQAATIAKIDFPEPPQLKTTAAFLSEGQPEKALADIEPVVKYY
                     TPLRDLPGNWWAQAALLKIGALGGMQLDKPAEALGDELRQRAVDPETARAAQLQLVAG
                     LLRKEDYAKALRLCDAVIKDSAKPEVLAEAWVRKGDALLAQRQWDGAVLAYLHVAVFY
                     EDEKLWMPPALLGRARAFRGLDDVERAKRSLDDLTAGYPKSAQAEIARTELQRLPK"
     gene            complement(5520..8366)
                     /locus_tag="VNW28_09645"
     CDS             complement(5520..8366)
                     /locus_tag="VNW28_09645"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA10235.1"
                     /translation="MRPLVRKKRATWLAILFFGLATNGSAQDASNLATAHSQAMAEFQ
                     AGNFARAATELEALVARVEVTPQAEPIFYTIGSAYFNAGDYPRAIAAFKNYQTKFPQG
                     AHAAAVAFAIAQSNLLSKNFKDAAAQMAVLENDPKLREQALLFEAEAFKATGRMDEAI
                     RALEKLAGTEIRTSDAMRGATMLAQLYAQKGDGAKALRVLEALHQKIALADNLIELNA
                     LTVDLGDKFYNTQQFKEALACYRYAGPREQIIQQQNDRVAGMQRRIEENLAAVRAHPA
                     EIVRLAPANNQLKDAIANALKLLAEFENLPSITPAIYLRLGRCFYELDRKWEAVVVDQ
                     EILDRFKEGPEREPALFGLIVALADVNQPQRAEERCEQYLREFKNGPNVGTVGYLLGA
                     VALQAGDPKAAETYFGRILETQPQNSFREQIRYLLGNAKFTAGNYGEAIAAYKKYLAE
                     FPHGANAEDVNYRITLCALFAGKYQDAMNQLGDYIARHPSGNFLPDAKYRLAVCNYAA
                     SLYDEVIADCRAWEKQFPGNPQLGEVLALLGDAYAASDRQVAAIPVYIRSYQTATTDE
                     VMNYSLFAASKLLQKQGDWIKVSELFGGFVKDKPDHPTVVQALYWIGKAKAREGKIDE
                     ARQLAADTIKKYINDPRRDAVEQLITQLAQFCVKKKTVVAGGADPGSGTGTSVQPEPV
                     SPAATNPLADLDRLLSGPNEESGTAKARILFGKAELARLQRQPDEEEKNIARIASDFR
                     PDDLSPLLLGAAGDCLLGKGKLDRAAGFYERLMDDFPKSQMVDYAYNGLGEIAYQKKD
                     YPKALRYFTDGTEKIAAAQKLKDISLGRAKTLLVLGKLDEAQKGFEQVASVREWRGEA
                     TVCSVYSLGQIAAKRGKWAEANAFYQRVYIGYQKFLPWVAKAYIASAESFEKLGKTQE
                     ATNTYRELLRNEKLTNFTEAIEARKRLEALGEGS"
     gene            8464..>8672
                     /locus_tag="VNW28_09650"
     CDS             8464..>8672
                     /locus_tag="VNW28_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10236.1"
                     /translation="MSEAKADVIREKSIAGLGGALLLILILVVYTNHFHNGFHYDDGH
                     TIVNNVSIRDLRNIPHFFRDATTFST"
BASE COUNT         1556 a         2551 c         2709 g         1856 t
ORIGIN      
        1 aaaagggccg cgccgacaag tgcgaccgcg ccgatctgcc ggcaacgcca gaccgccgcg
       61 cgcgttcccg cgagtttggt gaagaaaaat tcggccgaaa atgccagcgg cgtataatgc
      121 ccagcgacgc tttggcgcca gaatcggcgg atgttttgtt tctgttttgc cggcgacgaa
      181 tgggcgagga tctgataata tccgctgtcg gcacggagga aggtgatgtt ggtatcgtga
      241 tagagagcgt ccgtcacgat gaggcatgcg aagtaaacca gggcgaattt tcgcagggca
      301 gaagggatcg atcgcgatgc tgcagtctgg agcgattccg cgaatgggct caagcgccta
      361 tcttgagtcc acgccctacg atggtcaagg cgagcgccat tacttgcatt gcttcggccc
      421 gtgcataaat agtgctcccc tattcttcac atgagacagc ggttcgccat tcttttcctt
      481 attcttgccg cggccactgc ccaggcggca gagcgcgaat acattgtcct cgtgggcggc
      541 ccgtcgctcc acatctggga gcaatacaaa ggcccggtcg cgcacgacca ctggtgggcg
      601 aatttcgttc attcggcgcg ggtgcgcacc gagcagttac gggcccagct cggccccgat
      661 gcgatgatca cctggctggt ctacaaaccg ggctacgtcg atcgcggcgt gcaggaaaaa
      721 caggatctga tcgcgaatat caacagcgtg cgcgacaagt ttcatctcca tctcgtctac
      781 tttgatcgcg gcggggacgt gatcaattat ctgaacgaag ggcagccgcg gaaccagatc
      841 aaggtctcgg gcttcgaatt ttttggccac tcgaacaagg cctgtttcct cttcgactac
      901 agcaacgtcg tggatagcgc gtcgaaagcc tggttgcacg aaacggagct aaaaaaaatc
      961 gaccggcgcg attttacgcg cgacgcctac gtcaaaagct ggggctgcca caccggcgag
     1021 agcatgagca agctctggcg tgaagcgacc ggcacgcgga tgtggggcgc gatcggcaga
     1081 accgattact cgaccgggga attgcccgcg ctctcgtcgg tcggtggcag gtgggtgaac
     1141 tgagcgtagc gacgttttgc tctttctgag cgggaggctg cgaagcgcgg cggtgcaccc
     1201 tgcacggcat tgatgtttcc ccgcgcggct tctacaggta cggccctcgg ggtcggccga
     1261 actcgcggaa taatccgcct cgagtctcct cgtaagtctc gaagacggac cctgctcgcg
     1321 cgctcgattt ccttggcggc tccagccgcg tcgatttacg gtcgcagcgt ggcggcgctc
     1381 gacttcgaaa agctcgtgga agatttttac atgccgctct atcgcttcgc gttctcgctc
     1441 agccgccagg aatccgacgc ggccgacctc acccagcaga ctttttttct ctgggcctcg
     1501 aagggccatc agttgcgcga cgcgtcgaag gtaaaaacct ggctcttcac ctcgctttac
     1561 cgggaatttc tcgggcgcaa acggcagcag gaccgtttcg tggaatctga gaacaatacg
     1621 gaggcgatcg ccgcccaaac cgtcccggca tctgtcgtgg agcaactcga tggcgacatt
     1681 gtgcagcgcg cgctcctcgc gctcgaggag atctaccgcg cgccacttac gcttttctac
     1741 ctgcagcaac attcttacaa agagatcgcc gaaatcctca aggtgccgat cgggaccgtg
     1801 atgtcgcgca tttcgcgcgg caaagagcgc ctgcgccagg cgcttgccga cagcgccggc
     1861 ctaacgaagg aaaaccgggg aggaaatacg catggatgag caggaggcgc gattgattct
     1921 ccaggcctac cggctgggcg aggcggataa ggacgatccg catttcgacg aagcgctgca
     1981 ggaagcggcc cggaacccgg cgctcgcccg ttggtttgcg gaggagcagg cctttgatcg
     2041 cgcgctggcg gcgcatctcg actcgatgcc tgcgcctttt ggtttgaaga cccgcattct
     2101 ggcccaggcc gcgccacccg cgatgggcgg gagttcgcgc tggatcgtta gtctggccgg
     2161 cgtcgtcgcg ctcctttttc ttctcaccca ggtcgttagt ctgtggcgcg gctccgcgcc
     2221 cgcgcccgcg ctgcccgagt atgcggctga gatggtcagc ttcgtgcagt tgccgccgcc
     2281 gctcgatatg gagagtcagg atctgggcgc gatcaagcat tggctcgcgc aaaaggatgc
     2341 gctgccgccg gatgtcccgc cgcagctcgc cgcgcttcag ccagtgggct gccgggttct
     2401 ctcgttccgc ggccacgaag tgacactgat ttgtttcaag cgcggcggga accagctcgc
     2461 gcaccttttc gtggtcgatc gggcggcctt ccccgaggta aagccgggga cggcgccggt
     2521 ttttgcgagc gagggcgagt ggacaacggc gagctggatg gaaaaggatc gggtctacac
     2581 gatcgcggta caaggcgacg cgaatgcggt gcagcgttac ctgccgcatg cctaacgagc
     2641 ggcgaaatga gacttgctcc gattcccggc tgacctcaag gcgatgccgg ccccgctcgc
     2701 cgaagacgct actcttcttc gttcaccgtg aaggtgacgt tgtcgatctt ggcgagggcg
     2761 agcgcgtcga ggacgtcgat cgtgcggctg tacttcgcct gcctatcgct gatgatcgtc
     2821 accaccgccc gtgtcctggc ggcatcggcg ttttgtttca agcgcagcag ggtgcccttg
     2881 agttgtggca gatccttgct cgccgccgaa tcgaacggct cgtcgttgag caaaatctcg
     2941 ccggtatcgg agatggcgat gatctgttca tcgacaaaat cggtcgagga cgaggtttcc
     3001 gcggtcccgg gcagctgggt gttcagctcg ttctcgattt tcaccgcgcc ggccatgacc
     3061 atgaaaaaaa gcatgataac gaagacgacg tcgatcatcg gcgcgatctg gaaaccgatg
     3121 ttgccttcgt tgggattgag ctcgaggcgc acgattttaa atgacgaatg acgaaggtcg
     3181 aaggacgaag gaataacgaa tgacgaatga cctggctgtg gagcgagacg ctgtcgagct
     3241 gggtgaggaa gtggttcgcg cagcgaagcg ccatccccga ctcgaccgag tctcgcccca
     3301 ccatttcgtc attcggactt tggacattga ttcgtcattc gtcattcgga attcgtcatt
     3361 tcattcctta ttcaccgcgg agaacgaaat gtcggtgatg ccagcctccg cgcaggcgtt
     3421 catcgcctgg gaaacgtaaa tcgcgggcac gtcgcgatcg ccgcggatga ccgcgcggaa
     3481 cgtcgggttc gcgccggccg tcactttttt ctcgccggta atgcgggcat tcttgagcgg
     3541 ctcgaccatc tcggtcgctg cttgaaaagt gcgatcgtcg aacacgaatt gcgccttctt
     3601 cgcggggagc ttccaacgca cgttaaggat cgcttcagcg cgactgttgt cttttttctg
     3661 cgcgtccttc gcgatcggca actggatcga cttgtcgact ttcaagacct gcgcggaggt
     3721 gatcgtcatg aagaaaacga gaattgtcag gagcacgtcg atcatcggcg cgacctggaa
     3781 ttccggttca cctgcttcgc tgcctccgcc tccgcctgcc atcgtcttgt cttcgttagg
     3841 ctaactggtt ggtcgggttc cgctccggcg gcgcgtcggg gacgagccag ttcggcgtcg
     3901 cggcgaaaag ctcgtcatcc cccaaatgca caccggcgag cgactcgtag ggcattttac
     3961 ggaagaggcg gttgacgacg tcctggatgt ggtggagcga gtcggtcgcg cggttgcgga
     4021 ggaaataaaa tgcagcgaag gcggggatgg cgataatgag gccgctcgcg gtcgctacca
     4081 gcacctcgcc gatggccgcg gaaagaccgg aaggatcgcc aatcccgctc gagccgagct
     4141 tggcgaaggc cttgatcatg ccggtgacgg tgcctaacaa tccgatcatc ggggtggtga
     4201 cgccgataac cgagagatag ctgatgtagg tctgcatctg ggaattttcc ttggccagca
     4261 ccccgatcat gccgtcttcc gcgacttgtt tgccttcccc gaggaggccg atcccggagc
     4321 gcagcacgtt gctcaggggt gacggattgg ttttgcagta atcatgcgca ccgatgtagt
     4381 cgccctgccg aaaaagtgcc ttgagcgctt cctcctgctc gggcggcgcg gttttcttgc
     4441 ggctggtacg aatgattccg tcgccgatca gataaagggt cgcgatcgag caaagggcga
     4501 tcgggaacat gacccagccg ccttccttga tctgttccca gagcgttttc gagtgcacgc
     4561 cggcggctcc tgcgggcgag gcgtcctgcg caaaacccgg cgcggcgagg agcagaaaag
     4621 cgccggccag cgcagcggcg aaagcccggg cggtgcgttg cctggtcgtt aggcgaagga
     4681 tcgttcgaat catgtttttc attttggcaa cctctgcaac tcagtccggg cgatctccgc
     4741 ctgtgccgat tttgggtatc cggcggtcag atcgtcaagc gatcgcttcg cccgttccac
     4801 gtcgtctaaa ccgcgaaagg cgcgcgctct gccgaggagc gcgggcggca tccagagttt
     4861 ttcgtcttcg taaaacactg ccacgtggag ataagcgagg acggccccgt cccactgccg
     4921 ttgggcgagg agcgcatcgc ctttccggac ccaggcctcg gcgagtacct ccggtttggc
     4981 gctgtccttg atcaccgcgt cgcaaagtcg cagcgccttg gcgtaatctt ccttccgcag
     5041 cagcccggcg acgagttgca attgcgccgc gcgggcggtc tccggatcga ccgcgcgttg
     5101 gcgcagctcg tccccgaggg cttcggctgg tttatcgagc tgcatcccgc caagcgcccc
     5161 gattttgagc aaggccgcct gggcccacca attccccggc agatcgcgca acggggtgta
     5221 gtactttacg accggctcaa tatcggcgag ggccttttct ggttggccct cggaaagaaa
     5281 ggcagccgtc gtcttcaatt gcggcggctc cgggaaatcg atcttcgcga tcgtcgccgc
     5341 ctgataaccg acctcgccgc tgctcgcgcc gacctggatt ttggccatta ccatgtcgcc
     5401 ggcgcgccgg acgcctttgg tttcgattgt ctggccggtt ttgaggagga tgttttgggc
     5461 gtggatcggc ctaacgactg cgaggaggag gatgaaagga accgtgaaac ggagagtctt
     5521 catgatcctt ctcccagcgc ttcgagtcgt tttcgggcct cgatcgcttc ggtaaaattg
     5581 gtcagcttct cgttgcgcaa cagctcgcga tacgtgttgg tcgcttcctg ggttttgccg
     5641 agcttttcga agctttcggc gctcgcgatg taggctttcg caacccaggg caggaatttc
     5701 tggtaaccga tgtagacccg ctgatagaag gcatttgctt ccgcccattt gccgcgtttg
     5761 gcggcgattt ggcctaacga atagacgctg cagacggtcg cttcgccacg ccattcacgg
     5821 accgatgcca cctgctcaaa gcctttctgc gcttcatcca gtttcccgag cacgaggagc
     5881 gttttcgccc ggccgagcga aatgtctttc agcttttgcg cggccgcgat cttctccgtg
     5941 ccgtcggtga aataacgcag cgccttcgga taatccttct tttggtaggc gatttctccg
     6001 agcccgttgt aggcgtagtc gaccatctgg ctcttcggga aatcgtccat cagccgctca
     6061 taaaatcccg cggcccgatc caatttccct ttgcccagga gacaatcgcc cgctgcgcca
     6121 aggagcagcg ggctcaagtc gtccggccta aaatcgctcg cgatccgcgc gatgttcttt
     6181 tcttcctcgt ccggttgccg ctggaggcgt gcgagctcgg ctttgccaaa gaggatgcgt
     6241 gccttggccg tcccggattc ttcattgggt ccggaaagga ggcggtcgag atccgctagg
     6301 ggattcgttg cagccgggga gaccggctcc ggctggaccg atgttcccgt accggagccg
     6361 gggtcagcgc ccccggccac aacggtcttt ttcttcacgc agaactgcgc gagctgggtg
     6421 atcagctgct cgaccgcatc acgtcgcgga tcgttgatgt acttcttgat cgtatccgcg
     6481 gcgagctgtc tggcctcgtc gatcttgcct tcgcgcgctt tcgctttgcc gatccaataa
     6541 agggcctgaa ctacggtcgg gtgatcgggc ttgtctttga caaagccgcc gaaaagctcc
     6601 gagaccttaa tccagtcgcc ctgtttttgc agaagcttgc tcgcggcaaa gagcgagtaa
     6661 ttcatcacct cgtcggtggt cgcggtctgg taggagcgaa tgtaaaccgg gatggccgcg
     6721 acctggcgat cgctcgcggc gtaggcgtcg ccgagcaggg cgaggacctc gccgagctgc
     6781 gggttgccgg ggaattgttt ttcccaagcc cggcaatcgg cgatcacttc gtcgtagagt
     6841 gaggctgcgt agttgcagac ggcgagacgg tatttcgcgt cggggaggaa attgccgctc
     6901 gggtgtctgg cgatgtaatc cccgagctga ttcatcgcgt cctgatattt cccggcgaaa
     6961 agggcgcaga gcgtgatgcg atagttcacg tcctcggcgt tcgcgccgtg cgggaattca
     7021 gccaagtatt ttttgtaggc cgcgatcgcc tcgccgtaat tgccggccgt aaacttggcg
     7081 ttgcctaaca agtagcgaat ctgttcgcga aagctgttct gtggctgggt ctcaaggatg
     7141 cggccaaaat aagtctccgc cgccttcgga tcgcccgcct gcagggcgac cgctcctagc
     7201 aagtagccga ccgtcccgac gttcggcccg ttcttgaact cgcgcagata ctgctcgcat
     7261 ctttcctccg cgcgctgcgg ctggttcacg tccgccagcg cgacgatcag cccgaagagg
     7321 gccggttcgc gctccggccc ttccttgaaa cggtcgagga tttcctggtc gacgacgacc
     7381 gcttcccatt tgcgatcgag ctcgtaaaag cagcggccga gccgcaaata aatcgccggc
     7441 gtgatcgagg gcaggttctc gaattccgcg agtagcttga gcgcgttggc gatggcgtcc
     7501 ttgagctgat tattggccgg ggcgagccgg acgatttccg ccggatgggc gcggacggcg
     7561 gcgaggtttt cctcgatccg gcgctgcatg ccggcgaccc gatcgttttg ctgttggatg
     7621 atttgttcgc gcgggccggc gtagcggtag caggcgagcg cctccttgaa ttgctgggtg
     7681 ttataaaact tgtcgcccag atcaaccgtc agtgcgttca gctcgatgag gttgtcggcc
     7741 agggcgatct tttggtggag cgcctcgagc acgcgcagcg ccttggcgcc atcgcccttc
     7801 tgggcgtaga gctgggccag catcgtcgcg ccacgcatcg cgtcgctcgt ccggatttcc
     7861 gtgccggcga gtttttccag cgctcgaatc gcctcgtcca tcctgccggt ggctttaaag
     7921 gcttcggctt cgaagagcag cgcttgctcg cgcagcttgg gatcgttctc cagcacggcc
     7981 atttgcgcgg cggcatcctt gaagtttttg ctcagcaggt tcgactgcgc gatggcgaac
     8041 gcgacggcgg cggcgtgcgc gccctgcggg aacttcgtct ggtagttttt gaaggcggcg
     8101 atcgccctcg gataatcgcc ggcgttgaag taagcggagc cgatcgtgta gaaaattggt
     8161 tcggcctgcg gggtcacttc gacgcgggca acaagggctt cgagttcggt cgcggccctg
     8221 gcgaaattgc cggcctgaaa ttccgccatc gcctggctgt gggccgtggc gagattagat
     8281 gcgtcctgcg cggagccgtt ggtggccaat ccgaagaaca ggatcgccag ccaggtcgcg
     8341 cgttttttcc taacgagggg tcgcatgggg ggttgcggaa ctatagcaaa cccgccgcgc
     8401 gcaagtcgag cgatttgtcc agcgtcattg catcgacgga gtctttcgcc gtaaaatcga
     8461 ttagtgtcgg aggccaaagc ggacgtgatt cgcgaaaaat caatcgccgg tctggggggt
     8521 gcgctgctgc taatcctaat tctcgtggtc tacacgaacc attttcacaa cggctttcac
     8581 tacgacgatg ggcacacgat cgtaaacaat gtctccatcc gggacctgcg gaacattccg
     8641 catttcttcc gtgacgcgac caccttcagc ac
//
LOCUS       DATMNS010000275         6237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1350576, whole genome shotgun sequence.
ACCESSION   DATMNS010000275 DATMNS010000000
VERSION     DATMNS010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350576"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="VNW28_09655"
     CDS             <1..1518
                     /locus_tag="VNW28_09655"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HXA10237.1"
                     /translation="EEMRGRVAGGDLEREALFAQQIAPYRELVALLVRQKRPEEALVI
                     AEKSSARVLLDATDGGRTEWPSILTADERSRLQELNLKSTEANQVLTRLQAAAKLDEA
                     AIAAAATGLHEARGAQEDFATMIQSSHPELRRMTPPAALKTLTELEPTLRGGKTTLLR
                     FLVGERESFLFVVTLPDDSRTPVLKVISLGQGRAGLTRLTNDYRNSLAHRSLVWEKSA
                     RQLYDLLLRPAAAQLAGANSIIIVPDGPLWELPFQSLERAADDPLLADYSIRYAPSLT
                     LLAHAQKMAPVAPEHSLLAFINPALGSSPDARKPVLPDDEWEPLLGMEKQAGELAKIY
                     PAPAGEILVGSEAREKTFKEKAGGAAILHFATHGVLDDRAPLYSYLLLSQINLAPEED
                     GRLDVRELLQMKLHARLAVLCGCETARGEVTAGEGVVGLSWGFLVAGCPATIVSQWKV
                     ESASSTQLMVELHRQLHDRVENAEALRRASLKLREDPRYRHPFYWAAFVLVGANL"
     gene            complement(1540..2661)
                     /locus_tag="VNW28_09660"
     CDS             complement(1540..2661)
                     /locus_tag="VNW28_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10238.1"
                     /translation="MDENGHLTNAERAAYWQRSLAPSALLEVSDHLQECARCRGELRR
                     NRPTTPIAESVGYEDLVEWMDGDIDPLRRRELAERIGNSPAAAAELINLLQFRDEINE
                     LAEHDYAKAPQPRLMRSLSRAWPLAAGLVLGCAFLWLISAGHRAGADVALIDHGQRIV
                     VSAKGEISGLGALPPDLQRSVHNAISLGKVDRPALPGELRGPRETLAGAPSPQGSFRV
                     VAPVGSVVEGERPVFRWSKEPGATGYRVNLVPPGGAVVSSPLLPASATTWTPHEPLSP
                     NETYEWEVEALKDGETLAKSPAPPEPEARFRVLDAASRSELQQVREKWGVSHLVMGLA
                     YARVGLAAEARHEFQELARENPQTPLPKNLLAGVMDPEQ"
     gene            complement(2675..3760)
                     /locus_tag="VNW28_09665"
     CDS             complement(2675..3760)
                     /locus_tag="VNW28_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10239.1"
                     /translation="MFEPAQNQSASPGNGSDPLLAPYLAASSSAGTDTQLAQLITLHA
                     DPLIDVILRTRLARSGGLADGASNEEDIASAAREHLIGQLTALRQEAREPIRDFRAYV
                     ASTTYSAWAESLRARHPPRALLLNRLRYLLEGRSTQKGFSLWKDADGVKWCGLAPWTG
                     RLGGPSPKLQWLLVDADAALGEALGGLDPAQLVLPDLLARIFRWLGGPIELRDLTTAV
                     AGVPGVAGTPAHATGDACAAFDPRQSPAEDLIWKEYLGWLWSEIGALSDRQRCAFLLH
                     SEILREFELLGIASIRAVARALNLAAAELAELWNRLPLDDLTIAGMLNCTRQQVINLR
                     RVARDNLGEAWRVWKSGAGNKHAGSPS"
     gene            3926..>6237
                     /locus_tag="VNW28_09670"
     CDS             3926..>6237
                     /locus_tag="VNW28_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10240.1"
                     /translation="MKLPSLLLKLATITTLTLVAGHRLYGSDPEIFITGDGSQGGGIV
                     GAYTLAGGTLNATLVSGLGFPVAAVVSGGDLFVVDNENGTVGEYDATTGATINASLIS
                     GLADPKGIAISGSMLFVTVNNDMAFTAQVAEYTTGGVLVNGALITGLRLAVGIAVSGS
                     DLYVADFASGTVGQYTTSGTPINPSLITPSGRAYNLAVSGTSLFVISPEEATVYNYTT
                     SGDFVTSWQITDSIEPGSIAVSGSDVFVGDFSNKTVREYTTTGAIVNNALISGVSDLV
                     GMTISAVGAPTPTPTPTPKPTPTPTPTPVVGLSPTTFTVDGSANPTANVVDTVLRFAA
                     VQTGTPAGLSVRVQTSMTPTVGSSWTDLNNGSAGGMIYDISSKKFVSSSANYPLFNGV
                     YFRAIASAHGYPDSISNSVGPFSLASSTPRLSQPEISVTGNGPFADLYFRSYVATATN
                     GIALRVQSTTTPSNEVSWIDLNDGQAGHMKQSNEPNRFYLLANKLPAVTGVYFRVVAT
                     LTGHVDGLSAPFGPLNLVLDTPPTVTITPPKGISGSGTATSPLVLAAGTLHFSAQATL
                     PSGRKIKQISLLYDGSTINYSNISSVTVDYTTNVIGDHLVEATAVDDLGGSARDGTVP
                     LYIRVVPNTTTSAEKEETVVGGPRATQAATTGGKVFTVAKSGGNWNDPTTWKDNLGKT
                     GIPGEQDLAIVGSSSVKCPFDVVTGSVTLSGGQIIGPGTLDIFGVLSISSGAFVNSTL
                     FIGSGGVCNLTNSVPIQMAANVVNLGTWNV"
BASE COUNT         1185 a         1968 c         1892 g         1192 t
ORIGIN      
        1 gaggaaatgc ggggccgggt agcgggcggc gacctcgagc gggaagctct tttcgcgcaa
       61 cagatcgcgc cttatcgcga gctggttgcg ttactcgtta ggcagaaacg gccggaggaa
      121 gcactcgtca tcgccgagaa atcgagcgcc cgtgtgctcc tcgacgcgac cgacggcggc
      181 cgcacggaat ggccttcgat cctgaccgcc gacgaacgct cccggctcca ggagttgaat
      241 ttgaaatcga cggaggcgaa tcaggtcctc acgcgtctac aggccgcggc aaaactcgat
      301 gaagcggcga tcgcagcggc ggcaacgggt ttgcacgagg cccggggcgc acaggaggat
      361 ttcgcgacga tgatccaatc gtcgcatccg gagctgcggc gcatgacgcc gcccgcggcg
      421 ctaaagacgt tgaccgagct ggaaccgacc ttgcgcggcg gaaagaccac gctgctgcgg
      481 tttttggtcg gtgaaaggga gtcgttcctc tttgtcgtga ctttgcctga cgactccaga
      541 acaccggtgc tgaaggtaat ctcactcggc cagggccggg cgggattgac tcgcctaacg
      601 aacgattatc ggaacagcct ggcccaccgc agccttgttt gggaaaagtc ggcgcgccag
      661 ttgtacgatc tgctcctccg cccggcggcg gcgcaactgg cgggtgccaa ttcaattatc
      721 atcgtgcccg acggtccgct ctgggagctg cctttccaat cactcgagcg ggcggcggat
      781 gatccgttgc tcgccgatta ctcgattcgt tatgcccctt cgctcaccct tctcgcgcac
      841 gcgcagaaga tggcgcccgt cgcgcccgaa cattcgcttc tcgccttcat caatccggcg
      901 ctcggttctt cccccgatgc gcggaagccc gtcttgcccg acgacgaatg ggaaccgctc
      961 ctcggaatgg aaaagcaggc gggcgagctg gcgaaaattt atccggcgcc cgccggggag
     1021 atacttgtcg gaagcgaggc gcgagaaaaa acctttaaag agaaagccgg gggcgcggcg
     1081 attctccatt ttgccaccca tggcgtgctg gacgaccggg cgccgctcta ctcctacctc
     1141 ctcctttcac agataaatct cgcgccggaa gaagatggac ggctcgatgt gcgcgagttg
     1201 ctccagatga aattgcacgc ccgcctcgcg gtgctctgcg gctgtgagac agcgcgcggc
     1261 gaggtcacgg ccggcgaggg tgtagtcggg ctgtcctggg ggtttctggt cgcgggttgt
     1321 ccggcgacga tcgtgagcca gtggaaagtg gagagcgcga gcagtacgca gttgatggtc
     1381 gagcttcacc gccaactgca cgaccgtgtg gagaacgcgg aagcgctgcg gcgggcgagc
     1441 ctgaaactgc gcgaagatcc gcgctaccgg catccgtttt actgggcggc gttcgtcttg
     1501 gttggggcaa atctgtaact gcccgcagga tggctgagcc tactgctctg ggtccattac
     1561 gccggcgagc agattttttg gcaacggggt ctgcggattt tcgcgggcga gttcctggaa
     1621 ctcgtgccgc gcctctgctg ccaggccgac ccgcgcgtaa gccagaccca tgaccagatg
     1681 cgacacgccc catttctcgc gcacctgctg cagctcgctc cgcgaggccg catcgaggac
     1741 gcggaagcgc gcctccggct cgggaggggc cgggcttttg gcgagcgttt cgccatcttt
     1801 cagcgcctcg acctcccact cgtaggtctc gttaggcgac aggggttcgt gaggcgtcca
     1861 ggtggtcgcg gacgccggga gcagcgggct gctcaccacg gcgccgcccg gtggcacaag
     1921 attgactcga taaccggtcg cgcccggctc cttgctccag cgaaagacag gccgttcccc
     1981 ctcgacaacg ctcccgaccg gcgcgacgac ccgaaaagaa ccttgcggcg acggcgcgcc
     2041 cgccagggtc tcgcgcgggc cgcgcaattc gcccggcaaa gccggacgat cgactttgcc
     2101 taacgaaatc gcattgtgca cggaacgctg caaatccggc ggcagcgcgc cgagcccgga
     2161 gatctcaccc ttcgcgctaa cgacaattcg ttggccgtgg tcgatcagcg ctacgtctgc
     2221 gccggcgcgg tgtcccgccg agatcaacca gaggaacgcg caaccgagga cgaggccggc
     2281 cgcgaggggc cacgcgcgac tgagcgaacg catcaagcgc ggctggggcg ctttggcgta
     2341 atcgtgctcc gcgagttcgt ttatctcgtc gcgaaactgc aggaggttaa tcaactcggc
     2401 ggccgcggcc ggcgaattgc cgatccgttc cgccagctcg cgccggcgca gcggatcgat
     2461 gtcgccgtcc atccattcta ccagatcttc gtagccgacg ctctcggcga tgggtgtggt
     2521 ggggcgattc cggcgcagtt ccccccgaca gcgcgcgcat tcctgcaaat gatcgctcac
     2581 ctcgagaagc gcggacggcg cgagactccg ctgccaataa gccgcgcgtt ctgcattcgt
     2641 taggtgcccg ttctcgtcca ttgctttctc ctctctagga tggggagccg gcgtgtttat
     2701 tacctgcgcc agatttccag acgcgccacg cctctcccaa attgtcgcgc gccacccggc
     2761 gcaagttgat gacctgctgg cgagtgcagt tgagcatgcc ggcgatcgtt aggtcgtcga
     2821 gcggcaggcg attccagagc tctgccagtt cggccgccgc caggttgagc gcgcgcgcga
     2881 ccgcgcggat cgaggcaatg ccgagcagct cgaactcgcg gaggatttcg gagtggagga
     2941 gaaaggcgca ccgctgccga tccgaaagag ctccaatctc gctccagagc cacccgagat
     3001 attccttcca gatcaaatct tccgccggag actggcgcgg atcaaacgcc gcgcaggcat
     3061 cgccggtcgc gtgggccggt gtgcccgcga ctccgggcac acccgcgacc gccgttgtca
     3121 ggtcgcgcaa ctcgatcggg ccgccgagcc agcggaaaat gcgcgcgagc aaatccggca
     3181 ggacgagctg agcgggatcg agaccaccca acgcctcgcc gagggccgcg tcggcatcga
     3241 ccagcaacca ctgaagttta ggcgacggtc cgcccaaccg tcccgtccat ggagcgagcc
     3301 cgcaccattt cacgccatcc gcatctttcc agagagaaaa tcctttttgt gtgctccggc
     3361 cttcgagcaa ataacgaagc cggttgagga gcaacgcacg tggtggatgc cgggcgcgca
     3421 gcgattccgc ccacgctgaa tacgtggtgc tcgcgacgta agcgcgaaag tcgcggatcg
     3481 gttcgcgcgc ttcttgacgg agcgcggtga gttgcccaat cagatgctca cgggcggcgc
     3541 tggcgatgtc ttcctcattt gaagcaccgt cggctaaccc accgctgcga gcgagtcggg
     3601 tgcgcaggat gacgtcgatc agtggatccg cgtgcagggt gatcagctgg gccagttgcg
     3661 tgtccgttcc cgctgaagaa gacgccgcaa gataaggggc gagaagcgga tcggatccgt
     3721 tgcccggcga agcggactgg ttttgcgcgg gttcaaacat cttggaatca gcagaagccc
     3781 agcacctttc gagcgcgcga ccaacggttc tgtccgagag gcgcgagatt tttcacgtgg
     3841 ccggtaatat cgccgcccct tccgcatctc caagcagtga aatccaaact gtgtctctgc
     3901 tgaaaaggcc accaaacatc atacaatgaa acttccttct ctccttctta aactggcgac
     3961 gattacgacg ctcactctgg tcgccggaca tcgtctttac ggcagcgacc cggaaatttt
     4021 tatcaccggc gacggttcgc agggtggcgg gattgtcggt gcctacacgc tggcgggcgg
     4081 gacgctaaac gcgactctcg tctcgggctt aggattccct gtcgccgcgg tggtgtccgg
     4141 tggcgatttg ttcgtagtgg ataatgagaa tgggacggtg ggcgaatacg acgccacgac
     4201 gggcgcgacg ataaacgctt cccttatttc tgggctggcg gacccgaagg gcatcgccat
     4261 ttcgggctct atgttgttcg tcacggtcaa caacgatatg gcctttaccg cgcaagtcgc
     4321 cgaatacacc accggaggcg tgctggtgaa tggagcgctc atcacgggat tacgattagc
     4381 ggtggggatc gccgtttccg ggtccgattt atacgtcgcg gatttcgcct ccggaactgt
     4441 gggccaatat accacctccg gaaccccgat taatccctcg ctaatcactc ccagtggtcg
     4501 tgcgtacaac ctcgccgtct cgggcaccag cctgttcgtg atcagtccag aggaggcaac
     4561 tgtttacaat tacaccacct cgggagactt cgtgacctct tggcagataa ccgacagcat
     4621 cgaaccgggc agcatcgccg tctcgggctc ggatgtcttt gtcggggact tttctaataa
     4681 gaccgtccgc gaatacacga ctaccggggc catcgtgaac aacgccttga tttccggagt
     4741 cagtgacttg gtcgggatga cgatcagcgc ggtcggggcc ccgacaccga caccaacccc
     4801 gactcccaag ccaaccccga cgcccacccc gacaccggtg gtaggacttt ccccaactac
     4861 ctttaccgtc gatggaagcg ccaatcctac cgcgaacgtg gtcgacacgg tcctccgttt
     4921 cgccgccgtc cagacgggca ccccggccgg cctctccgtc cgggtgcaga ccagcatgac
     4981 gccgaccgtt ggatccagtt ggaccgacct gaataacggc agtgccggcg gtatgatcta
     5041 tgatatctcg agcaagaagt tcgtcagcag ctcggctaac tatcctctgt tcaatggagt
     5101 ttactttcgc gccatcgcct cggcccatgg ttatcccgat agcatctcca actctgtcgg
     5161 acctttcagt ctcgccagca gcactccacg cctgagccag ccggagattt cggtcaccgg
     5221 caacggccct tttgccgacc tctacttccg ctcctatgtc gcgaccgcga ccaatggcat
     5281 tgccctgagg gtgcaatcta ctaccactcc gtcgaatgag gtgagctgga tcgatttgaa
     5341 tgatggccag gccggccaca tgaaacaaag caacgagcca aaccgctttt acttgctggc
     5401 gaacaagctc ccggccgtga ccggcgttta cttccgcgtc gtggccaccc tcacgggcca
     5461 cgtggatggc ctttccgctc cgttcggtcc gctcaatctg gtcctcgata cgccgcccac
     5521 tgtcacgatc acgcctccca agggcatatc gggcagcggc actgcgacga gtccgctcgt
     5581 cctggcggcc ggaacgttac actttagtgc gcaagcgacc ctgccaagcg gacgcaagat
     5641 caagcaaatt agcctcctgt atgatggttc cacgattaat tattccaaca tttccagcgt
     5701 gaccgtggac tacacgacca atgtgattgg cgaccatctc gtcgaggcta ccgccgtgga
     5761 tgacttggga gggagcgctc gcgatggcac agttcctctg tatatccggg tcgtcccaaa
     5821 caccaccacc agcgccgaga aagaagaaac ggtggtcggt gggccgcgcg cgacccaggc
     5881 tgccaccacc ggtggcaagg tctttaccgt cgccaaatct ggtggtaact ggaatgatcc
     5941 caccacttgg aaagataacc ttgggaagac tggtattccc ggtgaacagg atctcgcgat
     6001 cgtcgggtcc tcctccgtca agtgcccatt cgatgtcgtg accggctcag taactcttag
     6061 tggcggccag attattgggc ccggaacgct tgacatcttc ggagtcctct ccatctcctc
     6121 aggcgcattc gtaaattcga ctctctttat tggctcgggg ggagtttgca atctaaccaa
     6181 tagcgtcccc atccaaatgg ctgcgaacgt ggttaacctc gggacttgga atgtgca
//
LOCUS       DATMNS010000276         2006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3147664, whole genome shotgun sequence.
ACCESSION   DATMNS010000276 DATMNS010000000
VERSION     DATMNS010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3147664"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VNW28_09675"
     CDS             <1..1158
                     /locus_tag="VNW28_09675"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA10241.1"
                     /translation="RFHEYPLSVQHLNRAVDLGQTNGAGSDSVLRSASELLEIDYTLG
                     NLRDTLPRYEAVLAAGEGRKAPNDVALLELEERVARARYRLGDWSQAAQGLGSLLKAQ
                     GTSAPPSEFVGEIELDLGQVLGDLARSADAEVHLRRAIEILTREIGAMHAKVAEARAA
                     LGRSLADTGQFDDAAEQFDKAQELAAGSFPLETGIAVRTRYFRALLFLQMDAPEKAEP
                     LLAQIVASQDAHSAADLKARTGSAPEVDRTGPVRRALGEAYAREGKIDAAIIALQRAV
                     AISERADGAQHPDTQSTRLSLAECLVAEGRDAEARAILTTPTMNLAALPAVHPIVAQL
                     RRVNGLLAQHEGNVEQARKSFGDSLAILQSLYGSQHWRVIRARQEFERAVP"
     gene            complement(1208..>2006)
                     /locus_tag="VNW28_09680"
     CDS             complement(1208..>2006)
                     /locus_tag="VNW28_09680"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HXA10242.1"
                     /translation="RDELLDSSWKPWLPEGGFHFLTKDAPYVGGDWTIEQIMRTIPDT
                     PSPPLFKKTEGAESVSLPFHEMETDSFYQTLHARRTQRKFSEAEVPLDSISRLLQTTW
                     GVQAYLKSKYFGSLPLKTSPSAGARNPIEVYLMALRVKGLDAGLYHYDARDHALEKIS
                     GEATPQLARAYCADQPFVAYSSALFIMTGVFARTMWKYPHPRAYRVVLLDAGHLGQTF
                     CLTATRMGLAPFSTAALKDSLIEKDLGIDGISESVIYVTGVGLPAGR"
BASE COUNT          415 a          613 c          592 g          386 t
ORIGIN      
        1 cgatttcacg aatacccact gtccgttcaa cacctcaatc gcgcagtgga cctagggcaa
       61 acaaacggcg caggatccga ctccgtacta cgaagcgcct ccgaattgct cgagatcgac
      121 tacacgctcg gcaacctgcg agacaccttg cctcggtacg aagccgtcct tgcagcaggc
      181 gaaggacgta aggcacccaa cgatgtggct ctgctggaac ttgaagaacg ggtggcgcgc
      241 gcacggtacc gcttgggcga ctggtcgcaa gccgcccaag gtctagggag tctcctgaag
      301 gcccaaggca catccgcgcc tccatccgag tttgttggcg agatcgaact cgacctcggg
      361 caggtgctcg gcgatttggc caggtccgcc gatgcagagg ttcacttacg ccgggcgatc
      421 gaaatcctta cgcgcgaaat tggtgcgatg catgcaaaag tagcggaagc acgggcggcc
      481 ctcggtcgat cgctcgccga caccggtcag ttcgatgatg cagcggagca attcgacaag
      541 gcgcaagaac tggcggctgg ctcctttccc ctagagacgg ggattgccgt gcgcacgcga
      601 tatttccgcg cgcttctatt tctccaaatg gacgccccgg aaaaggccga acccctcctc
      661 gcgcaaatcg tcgcgtccca agatgcgcat tctgcagcgg atctgaaggc gcgaaccggt
      721 tcggcgccgg aggtggatcg cacgggtccg gtgcggcggg cgctcggcga agcttatgca
      781 cgcgaaggca aaatcgacgc cgcaattatc gcgctgcagc gcgccgtcgc gattagcgaa
      841 cgcgcagacg gcgcacaaca tccagacact cagtcgacac gtctttctct cgccgagtgc
      901 ctcgtggcgg aaggacgcga cgcggaagct cgtgccatcc tcacgactcc gaccatgaat
      961 ctggcagcgc ttcccgccgt gcaccccatc gtcgcacaat tacgtcgggt gaatggcctc
     1021 ctggctcaac acgaagggaa tgttgagcag gcgcgaaagt cgttcggtga ctcgctcgca
     1081 atccttcaat cgctgtatgg atctcagcac tggcgcgtca tccgtgcgcg ccaggagttt
     1141 gagcgagcgg tgccataagg agtgacgtct cgccccaacg gcgcgccggc cactgcctga
     1201 cacgtgttca cctgcctgcc ggcaggccga cgccggtgac atagatcacc gattccgata
     1261 tgccgtctat tccaagatcc ttctcgatca gagaatcttt caaagccgcg gtactgaaag
     1321 gcgcaagacc catccgggtt gccgtcagac agaatgtctg gccgagatgg ccggcatcca
     1381 gcagcaccac tctataggcc cgaggatgag ggtacttcca catggtccgc gcaaagacgc
     1441 ccgtcatgat gaagagtgcc gacgaatagg cgacaaacgg ctggtctgcg caataagccc
     1501 gcgcgagttg cggtgtcgcc tcgccggaga ttttttcaag tgcgtggtcc ctcgcgtcat
     1561 agtgataaag cccagcatcg agtcctttca ccctgagtgc catcaaatac acttcaatgg
     1621 ggtttcgtgc gccggcagac ggacttgtct tgagcggcag ggacccgaaa tattttgact
     1681 tgaggtacgc ttggacgccc caagtcgttt gcagcagcct cgatatgcta tcgagcggta
     1741 cttccgcttc cgagaatttt cgctgcgtcc tccgggcgtg cagcgtctgg taaaagctgt
     1801 cggtctccat ttcatgaaac ggcagtgaaa ccgattcggc gccttcggtc ttcttgaaca
     1861 gaggcggcga tggcgtgtcg gggatcgtcc gcattatttg ctcgatggtc cagtcgcctc
     1921 cgacgtaggg tgcgtctttc gtcagaaagt gaaatccgcc ctcgggcagc cacggcttcc
     1981 acgaggaatc gagcaactca tcgcga
//
LOCUS       DATMNS010000277         8372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3148657, whole genome shotgun sequence.
ACCESSION   DATMNS010000277 DATMNS010000000
VERSION     DATMNS010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 8372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8372
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3148657"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VNW28_09685"
     CDS             complement(<1..746)
                     /locus_tag="VNW28_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HXA10243.1"
                     /translation="MNNFTPRAQQVLALARKEADRFNHNYVGTEHLLLGLIKLGQGVA
                     VNVLQKMGLDLETVRMEVEKQVGSGPETKMVGNIPYTPRVKKVLALAGKEARALNHSY
                     VGTEHILLGLLREGEGVAARVLKSLEVDIERTRNEILKELDPNFTPPENDPELGAPGE
                     TSKKDVKTPALRAFGRDLTDLAKKSELDPVIGRHNEIERVIQVLCRRTKNNPVLLGEA
                     GVGKTAIVEGLAQEIASGNVPELLREKKVIT"
     gene            complement(739..1863)
                     /locus_tag="VNW28_09690"
     CDS             complement(739..1863)
                     /locus_tag="VNW28_09690"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0046314 - phosphocreatine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HXA10244.1"
                     /translation="MKFSQIMSSAGEWVRGEGPHHQIVVSSRVRLARNLRHRAFPGWA
                     KKAERSQILEQIKPRVEELPEMQDSFSELLQDLSALEKQVLVERHLISREHAAKSIGS
                     AVVMNRKQTLSIMINEEDHLRMQSIRSGLQLKSAFKLVDKIDSTLEAKLDIAYDQRLG
                     YLTACPTNVGTGMRASAMLHLPGLVLSDLINQVIQAVNKIGLAVRGLYGEGTEAMGNL
                     FQISNQTTLGEKEDEIIARLTKVIETIIEKEHDARQMLIQKKSDTLWDQIGRAYGVLT
                     YAHAMTSKEALNLLSVMKLGVDLGAFPEDRRLPVDELFIETQPAHLQKTSQQKLNAEE
                     RDFLRAQIIRDRLKLFPKPDMGKVAREPADKPPSESNHNE"
     gene            complement(1868..2359)
                     /locus_tag="VNW28_09695"
     CDS             complement(1868..2359)
                     /locus_tag="VNW28_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HXA10245.1"
                     /translation="MLCDVCKCNDATVFLTQILEGKMQKVNLCDACSKEKGVQDPTGF
                     ALADLLLGIGAAEEIEKGAPTQKCPVCGFTQADFKKTGRLGCSACYETFSEGLGTLLK
                     AMHKGTEHVGKLPERAARQMKLTDKMRALNDHLEKAVAEENYETAATLRDQIKKLEHE
                     MAS"
     gene            complement(2450..3424)
                     /gene="ilvE"
                     /locus_tag="VNW28_09700"
     CDS             complement(2450..3424)
                     /gene="ilvE"
                     /locus_tag="VNW28_09700"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HXA10246.1"
                     /translation="MLTKDAAATAVATPPIKSVSAQKAAVQSSATKSPQIYIDGEFFD
                     QADAKVSVFDHGLLYGDGVFEGIRFYNGRVFRMEEHLERLWESAGSICIDIPMSRSEM
                     DEALLETIRQNGLRDGYVRLIVTRGVGNLGLNPIHCKRPSVIIIASTIALYPEEIYRS
                     GLTVVTVPTRRMGPATLNPAIKSLNYLNNVLARIEANLANADEALMLNDAGNVAECTA
                     DNVFLVKRGQIMTPPITAGALRGITRSVVFDIAAEFGIKISEPEVTRHDLYIADEAFL
                     TGTAAEVVPMIKVDGRTIGNGKPGAITLRTIARFRELTRETGTPIFRE"
     gene            complement(3473..4141)
                     /locus_tag="VNW28_09705"
     CDS             complement(3473..4141)
                     /locus_tag="VNW28_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA10247.1"
                     /translation="MPPPQLVARDLRRSFQIGRRRIDVLRGVSLEVQHGESIFLCGAS
                     GAGKTTLLYTLAGLERPESGEVEFEGQKLYRGGESALARLRNQKMGFVFQGYFLLPEL
                     TALENVMLPGIIGRRPAKEACAKSLAAVGLADRENHLPPELSGGEQQRVAIARALTND
                     PDIIFADEPTGNLDSKTGDAIMDLLLGLAHERRKTLVVVTHDNNLAERGDRTLRIVDG
                     LLAR"
     gene            complement(4322..4843)
                     /locus_tag="VNW28_09710"
     CDS             complement(4322..4843)
                     /locus_tag="VNW28_09710"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HXA10248.1"
                     /translation="MKRLTLGFVILALALTQSGQADPVRGRHPRAPQRAAAVHHFDRN
                     SFAVANRNVIRVRHDRAWWVARFPHTRFVLFGGGYFYWWGGYWYPAFGYDPYYSTYAY
                     DGPIYGYNNYAPGQVIENVQMALRDQGYYRGAIDGLIGPQTRAALARYQQSHGLVVTY
                     AVDEPTLVTLGLA"
     gene            complement(4890..5396)
                     /locus_tag="VNW28_09715"
     CDS             complement(4890..5396)
                     /locus_tag="VNW28_09715"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HXA10249.1"
                     /translation="MTDSGDKMFNVVLVEPEIPPNTGNIGRLCLATRSTLHLVRPLGF
                     STDDRTLKRAGLDYWAEVDVRFWNSLVELQAAQPPGARYFYLTTKSSRPYHAIKFQRA
                     DFLVFGRETKGLPEALLRANADYLLTIPMHGTRSLNLATAVAIVLFEAIRQLGDSEGI
                     NAASEGFE"
     gene            complement(5461..6450)
                     /gene="tal"
                     /locus_tag="VNW28_09720"
     CDS             complement(5461..6450)
                     /gene="tal"
                     /locus_tag="VNW28_09720"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF009001.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HXA10250.1"
                     /translation="MSTAVAEEEKTKLSQLEQLKKFTVVVADTGDFESISEFQPRDAT
                     TNPSLIYAATQKEQYFHLLDKVLADRKSSGLSGAAQIEDVIDHLLVEFGSEILKIVPG
                     RVSTETDARLSFDVEGSINKGRQLIKLYEKRGIPRERILIKIASTWEGIAAGEVLEKE
                     GINCNLTLMFSLPQAVRCAESGIELISPFVGRIYDWYKAANKREYTGAEDPGVQSVKE
                     IYTYYKKFGYKTEVMGASFRNVGQILELAGCDLLTISPDLLDKLSHSYEPIERKIGPN
                     RSKDAAIERLEMDEKKFRYLFNDNAMATEKTAEGIRKFAADIVKLEKFVAGKL"
     gene            6528..6857
                     /locus_tag="VNW28_09725"
     CDS             6528..6857
                     /locus_tag="VNW28_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10251.1"
                     /translation="MQKSDAPFEEVKREARLLGEPMNEQDQPPPKLRFAETPEEQKKW
                     MAQWRSAEVALLEVKREELRALSDEEAQAACDLLLANADEFYVPPQMRTSSGLVEQQR
                     IFMRARR"
     gene            6857..7429
                     /locus_tag="VNW28_09730"
     CDS             6857..7429
                     /locus_tag="VNW28_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10252.1"
                     /translation="MLNPLFAAAVRVQEFCNARAWRTCYIGGICVQRWAEQRQTKDGD
                     LTLLTGFDQDEEYVDELLQHFQPRREDARAFALRNRVLLIYDVSGIPFDVALAGLPFE
                     ERTVERATPWKLKSGAHLITCSAEDLVVHKAFAGRNLDWSDIERILQRQGQRLNFRLI
                     FEELRPLLELKEEPENEEKLRQLMKREGLL"
     gene            7651..>8372
                     /locus_tag="VNW28_09735"
     CDS             7651..>8372
                     /locus_tag="VNW28_09735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HXA10253.1"
                     /translation="MNSSTPLLSFCIPTRNRARFLGEFLESVAAQITPEAEVVISDDG
                     STDDTPAVIECFRTRIPRLVAQRVDPPLRYDRNLLHVVSLARGKYCWLFGDDDRLEPG
                     GLAAVLEALKVEPDLAGLTTDRVSYDSALANKLPVRALKQQQSALFLKAEEAFLGLLD
                     RLGFLSCQIVNRAIWNEIVSLENLEPYFTGYVQLYVIARLLVQAPNWQFLAAPCVAFR
                     ADNDSFRALGQLGRLKMDVCGY"
BASE COUNT         1587 a         2531 c         2483 g         1771 t
ORIGIN      
        1 gtgatgactt tcttctcccg cagtagttcc ggcacgttgc cgctggcgat ttcctgcgcg
       61 agaccctcga cgatcgccgt cttgccgacc ccggcttcgc ctaacaagac cgggttgttt
      121 ttcgtccggc gacagagcac ctgaatgacg cgctcgattt cgttatgccg gccgatgacc
      181 ggatcgagct cgctcttctt cgcgagatcg gtcaggtcgc ggccaaaggc gcgaagagcc
      241 ggggtcttga cgtccttctt ggaggtttcg cccggcgcac ccagctccgg atcgttttcc
      301 ggcggggtga aattgggatc gagttccttc aggatttcgt tgcgcgtccg ctcgatatcg
      361 acctcgaggc tcttcaagac ccgggcggcg acgccctcgc cttcgcgcag caggccgagc
      421 aggatgtgct cggtgccgac gtaggaatga ttgagcgcgc gcgcttcctt accggcgagc
      481 gcgagcactt ttttcacccg cggagtgtag ggaatgttgc cgaccatttt cgtctccggg
      541 ccggagccga cctgtttttc gacttccatc cgcacggttt cgaggtcgag ccccattttc
      601 tggaggacat tgaccgcgac gccctggccg agcttgatca ggccgagcag gagatgctcg
      661 gtcccgacgt aattgtggtt gaagcggtcc gcttctttgc gggcgagcgc gagaacttgc
      721 tgcgcccgag gagtaaaatt attcattatg gttagactcg gagggcggtt tgtcggcggg
      781 ctcccgcgcc actttaccca tatcaggttt aggaaagagt ttcaaacgat cgcggatgat
      841 ttgggcgcgc agaaagtcgc gttcttcggc gttgagcttc tgttgcgagg tcttttgcag
      901 gtgcgccggc tgagtttcaa tgaagagctc atcgaccggc aaccgccggt cttccgggaa
      961 cgccccgaga tccacgccga gtttcatgac cgagagcaaa ttaagcgcct ccttcgaagt
     1021 catcgcatgg gcatacgtga gcacaccgta tgccctgccg atctgatccc agagcgtgtc
     1081 ggactttttc tggatcaaca tctggcgcgc gtcgtgttct ttttcgatga tcgtttcgat
     1141 caccttggtc aggcgcgcga tgatttcgtc ctctttttcg ccgagcgtcg tctggttcga
     1201 gatctggaaa aggttgccca tcgcctcggt gccttcgccg tagaggccgc ggacggcgag
     1261 accgatcttg ttcacggcct ggatgacttg attgatcagg tcgctcagga cgaggcccgg
     1321 taaatgaagc atcgccgagg cgcgcatgcc ggtgccgacg ttggtcggac aggcggtcag
     1381 gtaaccgagc cgctgatcgt aggcgatgtc gagcttggct tccagcgtgc tgtcgatttt
     1441 atcgacgagc ttgaaggcgc ttttcaattg cagcccggaa cggatcgatt gcatccgcaa
     1501 atgatcctct tcgttaatca tgatgctgag cgtttgcttg cgattcatga cgaccgcact
     1561 gccgatgctt ttcgcggcat gctcgcggct gatgagatga cgctcgacga gcacttgttt
     1621 ctcgagcgcg gaaagatcct ggagcaactc ggagaacgaa tcctgcatct ccggcaactc
     1681 ttcgacgcgg ggtttgatct gctcgagaat ttggcttcgc tcggcttttt tcgcccagcc
     1741 ggggaaggcc cggtggcgca aattccgcgc cagtcgcacc cggctgctga ccacgatctg
     1801 gtggtgcggg ccctcgccgc ggacccactc gccggcgctc gacatgattt gggagaattt
     1861 cattcgatta actcgccatc tcgtgctcga gcttcttgat ctggtcgcgc aaggtcgccg
     1921 cggtttcgta gttttcttcc gcgacagctt tctccagatg gtcgttgagg gcgcgcatct
     1981 tgtcggtcaa tttcatctgg cgcgcggcgc gttccggcaa cttgccgaca tgctcggtcc
     2041 ccttgtgcat cgccttgagg agcgtcccca gaccttccga aaacgtctcg taacaggcgc
     2101 tgcaaccgag ccgcccggtc ttcttgaagt ccgcctgggt gaaaccgcaa accgggcatt
     2161 tctgggtcgg cgcccctttc tcgatctcct ccgcggcgcc gatccctaac aatagatcgg
     2221 cgagggcaaa cccggtcgga tcctgcaccc ctttctcctt cgagcaggca tcgcagaggt
     2281 tcaccttctg catcttgccc tcgagaatct gggtcagaaa aaccgtcgca tcattacatt
     2341 tacaaacgtc gcacagcatg gctgatcaat tagaccacaa actggtggga aaattcaacc
     2401 gccagaccgg cggcggcact ctttcgtctc ttgcccgacg cgcgtccgtt cactcccgaa
     2461 aaatcggcgt cccggtttcg cgtgtcaatt cccggaagcg cgcgatcgtg cgcagagtga
     2521 tcgcgcccgg cttcccgttc ccaatggtgc ggccgtcgac tttgatcatc gggacgacct
     2581 cggcggccgt cccggtgagg aaagcttcgt cggcgatgta gagatcgtgg cgcgtcacct
     2641 cgggctcgga aattttgatc ccgaattccg cggcgatatc gaagaccacc gagcgcgtga
     2701 tgccacgcaa cgcaccggcg gtgatcgggg gcgtcatgat ctggccacgc ttcacgagga
     2761 agacgttgtc cgcggtgcac tcggcgacat tgccggcatc attcagcatg agtgcttcgt
     2821 cggcgttagc gaggttcgct tcgatccggg cgaggacgtt gttcaggtaa ttcagcgact
     2881 tgatcgcggg attcaaggtc gccggtccca tccgtcgagt tggcaccgtg accacggtca
     2941 ggccgctgcg ataaatctcc tcgggataga gcgcgatcgt cgacgcgata atgatcacgc
     3001 tcggccgctt acagtggatc gggttgaggc cgaggttgcc gacaccgcgg gtcacgatca
     3061 accggacata accgtcgcgc agcccatttt gccggatggt ttcgagcagt gcctcatcca
     3121 tctccgaacg cgacatcggg atgtcgatgc agatagaacc ggctgattcc cagaggcgct
     3181 ccaggtgttc ctccatgcga aagacgcggc cgttgtaaaa gcgaatgcct tcaaaaacgc
     3241 catcgccgta gagcaggccg tgatcgaaaa ccgaaacctt ggcgtcggcc tggtcgaaaa
     3301 attcgccgtc gatatagatt tgcggagatt tcgtggccga agactgcacc gcggctttct
     3361 gcgcggaaac ggatttgatc gggggcgtcg cgacggcggt ggcggcagcg tctttggtta
     3421 gcatgaaaaa acctccggtc attcggaagc caattcaagc ggagaatcgg tcctaacgcg
     3481 caagcagacc gtcgacgatc cgcagggtgc gatcgccgcg ctccgcgaga ttgttgtcgt
     3541 gcgtgacgac gacgagcgtt ttccggcgct cgtgcgccag accgaggagc aggtccataa
     3601 tcgcgtcgcc ggtcttggaa tcgaggttcc cggtcggttc atccgcgaaa atgatgtcgg
     3661 gatcgttcgt tagggcgcgg gcgatcgcga cccgctgctg ttcgccgccg gaaagctccg
     3721 gcgggagatg gttctcccgg tcggccagac cgaccgcggc caggcttttc gcgcaggctt
     3781 ccttcgcggg acggcgaccg ataattcccg ggagcatgac attctccagc gcggtcaatt
     3841 ccgggagaag gaagtagccc tggaaaacaa aacccatctt ctggttgcgg agcctggcca
     3901 gcgccgattc gcccccgcga taaagcttct ggccctcaaa ctccacttcg cccgattccg
     3961 gccgctccag cccggccagg gtgtaaagca gagtcgtctt ccccgcgccc gaggcgccac
     4021 agaggaaaat cgattcgccg tgctggacct cgagagagac tcctcgcaag acatcgatcc
     4081 ggcgtcggcc gatctggaag gaacggcgca gatcgcgcgc gacaagttgt ggaggcggca
     4141 tcgggcagca tcattggcca actcaggggt ggccgcaagg gagaggcgca cctcgagcag
     4201 ccaacgccgc gcactgacgg ctcccacatt ttggccgcaa tggtcgctca agcttcgcgg
     4261 caaagcagaa gccgcgtgga ggatctcctc cacgcggcgc tgtgcatcca atccaacgtc
     4321 gttaggccag gccaagcgtg accagcgtgg gctcatccac tgcgtaagtc acaaccaggc
     4381 cgtggctctg ctggtaacga gccagcgcgg cgcgcgtttg cgggccaatc aggccgtcga
     4441 tcgcaccgcg gtagtagccc tgatcgcgca aggccatctg gacgttctcg atgacctgcc
     4501 cgggagcgta attgttatac ccgtagatcg gaccgtcata ggcataggtc gaataatacg
     4561 ggtcgtagcc aaacgccgga taccagtatc cgccccacca atagaaatag ccgccgccga
     4621 agaggacgaa gcgggtgtgc ggaaaccgcg caacccacca ggcacggtcg tgccggaccc
     4681 ggatcacatt tcggttggcg accgcgaagc tattgcgatc gaaatgatgg accgccgccg
     4741 cccgttgcgg cgctcgagga tgccgtccgc ggacgggatc ggcctggccg gactgtgtca
     4801 gcgccagagc cagaatgaca aatccaagtg ttaatcgttt catagtaaag agtccttgtt
     4861 ttctggctta gaccgtctgg acgcgccaat tattcaaacc cctcggacgc ggcgttaatt
     4921 ccctccgaat caccgagttg gcgaatggct tcaaagagaa cgatggccac ggcggtggcc
     4981 agattgagac tgcgcgtccc gtgcatcggg atcgtcaaca gatagtctgc atttgcccgc
     5041 aggagcgctt ccggaaggcc tttggtctcg cgcccgaaca ccagaaaatc ggcgcgctga
     5101 aacttgatcg cgtgataagg acgggacgat ttcgtggtca ggtagaaata gcgcgcgccc
     5161 ggcggctgcg cggcttgcag ttcaaccaag gaattccaaa atcggacgtc gacctccgcc
     5221 cagtagtcga ggccggcccg tttcaaggtg cgatcgtcgg tcgagaaacc gagcggccta
     5281 acgagatgca gagtcgagcg tgtcgccagg cagagccgtc cgatgttgcc ggtattcggc
     5341 ggaatctccg gctcgaccag gacgacgtta aacatcttgt cgccggaatc ggtcatcccg
     5401 gctttccgaa ttttcgcgct cggccgccca gctaatgccg gacgggatga ccgctactgg
     5461 ctacaacttg ccggcgacga atttctccag cttcacgata tcggccgcga acttgcggat
     5521 gccttcggcg gtcttttccg tcgccatcgc gttatcgttg aagagatagc ggaacttctt
     5581 ctcgtccatc tccagccgtt cgatggccgc atccttcgat cgattcggac caatcttgcg
     5641 ctcgattggc tcgtaggaat ggctcaattt gtcgagcaga tcgggactga tggtgagcag
     5701 atcgcagccg gccagttcca gaatctggcc cacgttgcgg aagctcgcgc ccatcacttc
     5761 ggtcttgtag ccgaacttct tgtaataggt gtaaatctcc ttcaccgatt gcacacccgg
     5821 atcctccgcg cccgtgtact cgcgtttgtt cgccgctttg taccagtcgt aaatccggcc
     5881 gacgaagggc gagatgagtt cgatgccgga ttccgcacaa cgcacggcct gaggcaatga
     5941 aaacatcagt gtcagattgc aattgatgcc ttccttttcc agcacttcgc cggcggcgat
     6001 cccttcccag gtcgaggcga tcttgatcag gatgcgctcc ctggggatgc cgcgtttttc
     6061 atagagtttg atcaactggc ggcctttgtt gatcgagcct tccacgtcaa aggagagccg
     6121 cgcgtccgtc tcggtcgaaa cgcgccccgg cacgatcttg agaatctcgc tcccgaactc
     6181 caccaggaga tgatcgatca cgtcttcgat ctgcgccgcc ccgctcaggc cggaactttt
     6241 ccgatccgcc aggaccttgt cgagcagatg aaaatactgc tccttctgcg tcgccgcgta
     6301 aatgagactc gggttcgtgg tcgcgtcccg cggctggaat tcgctgatcg attcgaaatc
     6361 tcccgtgtca gcgacgacga cggtgaattt cttcaattgc tcgagctggc tgagcttggt
     6421 tttttcttct tcggcgacgg cagtgctcat gatcggttct ccagtttttg agcgacgcgg
     6481 aaatcgcgcc ttccaaaaaa tactacgccc gccgagttcg tccgacaatg caaaagtccg
     6541 acgcgccgtt tgaggaagtg aaacgggaag ctagattgtt aggcgagccc atgaatgagc
     6601 aagaccaacc gccgccaaag ctgcgcttcg ccgaaacgcc ggaggaacag aaaaaatgga
     6661 tggcgcaatg gcgctccgct gaagtcgcct tgctcgaggt gaagcgcgag gagctgcgtg
     6721 cgctgagcga tgaagaggcc caggctgctt gcgacctcct gctggcaaac gcggacgaat
     6781 tctatgtccc gccgcagatg agaacctcga gtggtttggt cgagcagcag cggattttca
     6841 tgcgggcccg ccgctgatgt tgaatccact tttcgccgcc gccgtccgcg tgcaggaatt
     6901 ctgcaacgct cgagcctggc ggacctgcta catcggcggc atctgcgtcc agcgttgggc
     6961 agagcagcgc caaaccaaag atggcgacct cactttgctg accggcttcg atcaggatga
     7021 agaatatgtg gacgagttgc tgcaacattt tcaaccgcgc agagaagacg ctcgcgcctt
     7081 tgcgctacgg aaccgcgttc tcctcatcta cgatgtgtcc ggaattcctt ttgatgtcgc
     7141 tctcgctggt ttgccgtttg aagaacgaac ggtcgaaaga gcgactccct ggaagttgaa
     7201 atccggcgct catctcatca cctgcagcgc ggaagatctc gtcgttcaca aggctttcgc
     7261 cggccgaaat ctcgattggt ccgatattga gcgcatcttg cagcgacagg gacaaaggtt
     7321 gaattttcgt ctcatcttcg aagaactgcg gcctttgctg gagttaaagg aagagccgga
     7381 aaatgaggaa aagctgcggc agttgatgaa gcgcgagggc ttattgtaga ggcggcccgc
     7441 tctgcagcaa aggacggcag gcggagcgcc cgccctacaa ttcacgtcga ttgtgtcgcc
     7501 gtcgctttcc cgctacactc ggcgcgcgat catgaccacc gcgccgcccg aattggtctt
     7561 cgccacgtgt cgctgtggca gctttcgccg cttcgctcag cggaggcagc ggcagcctaa
     7621 tggccaggct cgccgcttaa gctcgcgccc gtgaactctt ccacaccgct tctctctttc
     7681 tgcatcccga cgcgcaaccg cgccaggttt ctcggcgaat tcctcgagag cgtcgccgcg
     7741 cagatcacgc ccgaggcgga ggtcgtgatt tccgatgacg ggtcgaccga tgacacaccc
     7801 gcggtgattg aatgtttccg cacgcgcatt ccgcggctcg tcgcgcagcg ggtcgatccc
     7861 ccgctgcgct acgaccgcaa cttgctccac gtggtctcgc tcgcgcgcgg caaatattgc
     7921 tggctcttcg gcgacgacga ccgcctcgag cccggcggcc ttgccgcggt cctggaggca
     7981 ctgaaggtcg aaccagacct ggctggccta acgaccgacc gcgtttctta cgacagcgcg
     8041 ctggcgaaca agctcccggt gcgcgccttg aagcagcagc agagcgcgct tttcctgaag
     8101 gccgaagaag ctttcctggg tttgctcgat cgcctcggct ttctttcctg ccaaatcgtc
     8161 aaccgcgcca tctggaacga gatcgttagc ctggaaaacc tcgagcccta tttcaccggc
     8221 tacgttcagc tatacgtcat cgcgcgcctg ctcgtgcagg cgccaaactg gcagttcctc
     8281 gccgcgccct gcgtcgcctt tcgcgccgac aacgattcct tccgcgccct cgggcaactg
     8341 ggtcgcttaa aaatggatgt ctgcggctac ga
//
LOCUS       DATMNS010000278         4077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1364544, whole genome shotgun sequence.
ACCESSION   DATMNS010000278 DATMNS010000000
VERSION     DATMNS010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1364544"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            272..766
                     /locus_tag="VNW28_09740"
     CDS             272..766
                     /locus_tag="VNW28_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HXA10254.1"
                     /translation="MRIEHLFTSPGHNYYGHHGQPPDDFPLVETTEIECVAGRGIRGD
                     RFFDYREDYKGQVTFFSREVFALMSATFGLTNKSPGVLRRNLIVSGIDLRELIGTDFE
                     LQGVQFRGTAHCAPCYWMDTAFAPGAEKFLAGRGGLRARILTDGRISLGAARLKILRS
                     AAPP"
     gene            769..1314
                     /locus_tag="VNW28_09745"
     CDS             769..1314
                     /locus_tag="VNW28_09745"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HXA10255.1"
                     /translation="MRCSAVLLAGGKSSRMGRDKAFLEFEGEPLWQRQIQTLINLSPE
                     QLMISGPSREEWNECEIVADEIAGAGPLAGVGAALRKCTAPLLVVLAVDLPRMSTDFL
                     RSLCTDSRGAVPRGPAGFEPLAAVYPAACAALATQALSTGDFSMQDFVRRAIDRGLLM
                     ERLIDEVESLFFANLNTPADL"
     gene            1311..2126
                     /gene="fdhD"
                     /locus_tag="VNW28_09750"
     CDS             1311..2126
                     /gene="fdhD"
                     /locus_tag="VNW28_09750"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HXA10256.1"
                     /translation="MKNRNTARAIEVVRYRDGHPLPAAPDEAAVEEPLEIRIEGQSVA
                     VVMRTPGHDRELAAGFALTEGIVHAAADIFEINSCLAKGESGGNELDIALTDPAKFNL
                     AKLSRHVFSSSSCGICGRATIESAMQQFPPIKAAPSLDAQVLLQLPAKLAAAQMTFQR
                     TGGLHACALFEASGELILLREDVGRHNALDKLIGHQLLARQLPLGNSILVLSGRVSFE
                     MTQKALAGGIAVIAAISAPTSLAIDFAHANKQTLIGFLRGDTMNVYAGAERLS"
     gene            complement(2241..3125)
                     /locus_tag="VNW28_09755"
     CDS             complement(2241..3125)
                     /locus_tag="VNW28_09755"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXA10257.1"
                     /translation="MNIAFVGVGRMGANMARRLKEKGFHVTAVYDADRPAATTLAQEL
                     GSFASQSLGKVTAAAEAIVTVVSDDAAMREIFASPDDNLLVNAQGRLFINCATISPEV
                     HLEVEGLAQKIGAQTLEACMASSITQAREGSLYLMCGGEKSAFDRAAPILQALGSKIR
                     YIGKSGQAAKVKALVNMVMNINTAGLAEGLGLGDALGLDLTMLREVFSETGAGSRVLQ
                     TDGEDMQNRDHACFFSAAHAAKDSGIALHLADLAGLQLPLARATKEQYNRMIFEGLGE
                     LDKSGIAELTFKGRGPSA"
     gene            complement(3129..3947)
                     /locus_tag="VNW28_09760"
     CDS             complement(3129..3947)
                     /locus_tag="VNW28_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXA10258.1"
                     /translation="MAIPLEDNFTDIMGKAQRGLGISDSQLAEKSGASVVAIRQLREG
                     RFDRAVSDQIAPVLQLHPGALADLAQNKWQPNEIEVPGLFQCNTPYHDVTVNAYLVWD
                     PASREAVVFDSGADCSPLLQRSAGAGLTIQLLLLTHAHPDHVADLRRLTRETGAPVFL
                     SSREQAPGAEPIEEGKILHLGRLRIEARLTWGHSPGGMTYVISGLARPVAIVGDSLFA
                     GSMGGGNVSYEEAIGNNLKKILTLPDETVLCPGHGPMTTVGEEKKHNPFFAGRI"
BASE COUNT          775 a         1250 c         1238 g          814 t
ORIGIN      
        1 gctcacgatc gcgcacccca gcgtcccggc ggccatccag ggatagcgtt tgaggtaggc
       61 gaagacgcgc cagacggtgt tcatggcaga acaccgtcaa cggtgggttg ggcgcgtcaa
      121 gtcggagcgg cgcgtggaaa agggagaaaa cgttcaacgc tcaatgtttc aacgaagaat
      181 cagagcgcga cgctttctcc tatttggacg ttgaacgttg ggcgttgaac gttgaacgtt
      241 ttcttctccc tcttctttcg gatcgttacc aatgcgcatc gaacacctct tcacctctcc
      301 cggccacaac tactacggcc accacggcca gccgccggat gattttcccc tcgtcgagac
      361 gaccgagatc gagtgcgtcg ccggccgcgg cattcgcggc gaccgctttt ttgattaccg
      421 ggaagattac aaagggcagg tcaccttttt ctcgcgcgaa gtcttcgctt tgatgtccgc
      481 aacttttggc ctaacgaata aatcgcccgg cgtactccgc cgcaatctca tcgtgtccgg
      541 gatcgatctg agggagttga tcggcacgga tttcgagctg caaggcgtcc agttccgcgg
      601 caccgcgcat tgcgcgccgt gttactggat ggacaccgct ttcgcgccgg gcgcggaaaa
      661 gttcctggcc ggccgcggcg gtttgcgggc gcgtattttg accgatggtc ggatcagcct
      721 gggcgcggcg cggttgaaaa ttctgcgatc ggccgcgccg ccctaacgat gcgctgcagt
      781 gccgtcctgc tcgctggtgg aaaatcgtcg cgcatgggtc gcgacaaagc ctttctcgag
      841 tttgagggcg aaccgctttg gcaacgccaa atccaaacgc tgataaactt gtcgcccgag
      901 cagttaatga tttctggccc atcccgcgag gaatggaacg aatgcgagat cgtggccgac
      961 gaaatcgccg gcgcaggacc gctcgcggga gtgggcgcgg ctttgcgaaa gtgcacggcg
     1021 ccgctcctcg ttgtgctggc ggtcgatctc ccgcgaatga gcacggattt tctccgttcg
     1081 ctctgcacag actcgcgtgg cgctgttcct cgcgggcccg ctggattcga accgctggcg
     1141 gccgtttacc cggcggcctg cgccgcgttg gccacgcaag cgctcagcac cggcgatttt
     1201 tccatgcaag attttgtccg ccgcgcgata gaccgcgggc tgctcatgga acgattaatc
     1261 gacgaagtcg agagcctgtt tttcgccaat ctcaacacgc cggccgacct gtgaaaaacc
     1321 gcaacaccgc gagagcaatc gaagtcgtca ggtatcggga cggccacccg ctccccgcgg
     1381 cgccggacga agccgcggtc gaagagccgc tcgagattcg aatcgagggc cagagcgtcg
     1441 cggtcgtaat gcgaaccccg ggacatgatc gcgaactcgc cgccggcttt gccctgactg
     1501 aaggaatcgt gcacgccgcg gcggacattt tcgagatcaa ttcctgcctg gcgaaaggcg
     1561 aatccggcgg aaatgaattg gacatcgccc tcaccgatcc ggcgaaattc aatctcgcca
     1621 aattgagccg ccatgttttc agttcctcga gctgcgggat ctgcggcagg gcgacgatcg
     1681 aatcggccat gcagcaattt ccaccgatca aggccgcgcc ctcgctcgac gctcaagtgc
     1741 tgcttcagct cccggcaaag ttagccgccg cgcaaatgac atttcagcgc accggcggcc
     1801 tgcatgcctg cgcgctcttc gaagcgagcg gggaactgat tcttttgcgc gaggatgtcg
     1861 ggcggcacaa cgcgctcgac aagttgatcg gccatcagtt gctcgccagg cagctcccgt
     1921 taggcaactc gattcttgtc ttgagcggac gcgtctcctt cgagatgacg cagaaagcgc
     1981 tcgccggggg aatcgccgtg atcgccgcga tttccgcccc gaccagcctc gcgatcgatt
     2041 ttgctcacgc caacaagcag acgctgatcg gttttctgcg cggcgacacg atgaatgtct
     2101 acgccggcgc tgagcgactg tcgtaacaca gccgtcacgg ctgtcgggtc gaccgggcgt
     2161 cctcgcccgg caggctccgc gcgcaagaaa aaacgaccgg tcaagatgct cgcccgacat
     2221 gacaggccgg gaggcctgtg ttacgcggag gggccgcggc ctttaaacgt cagctcggca
     2281 atgcccgatt tgtcgagttc gcccagaccc tcaaagatca tccggttgta ttgctctttg
     2341 gtcgcgcggg cgagaggaag ttgcaggcct gccaggtccg ccagatgcaa agcgatccca
     2401 ctatccttcg ccgcatgcgc ggcggagaaa aaacaggcgt gatcgcgatt ctgcatatct
     2461 tcgccgtcgg tctgaagaac gcgcgagccg gcgccggttt cgctgaaaac ttcgcgcaac
     2521 atcgtaagat cgagcccaag cgcgtcgccc aatccaagac cctcggccag gccggcggtg
     2581 ttgatgttca tcaccatgtt gaccagcgcc ttcactttcg ccgcttgtcc cgacttgccg
     2641 atgtagcgaa ttttggaccc aagggcttgc aaaatcggcg cggcgcgatc gaaggcggat
     2701 ttttcgccgc cgcacatcag gtaaagcgag ccttcgcgcg cctgtgtgat gctcgaggcc
     2761 atgcaggctt cgagcgtctg cgcgccaatt ttttgcgcga ggccctctac ctcaagatgc
     2821 acttcgggag agatggtagc gcagttgatg aagagcctgc cctgcgcgtt gacgagcagg
     2881 ttgtcgtcag gtgaggcgaa gatttcccgc atcgcggcgt cgtccgaaac gacggtgacg
     2941 atagcctcgg cggcggccgt tactttgcct aacgactggc tggcaaaact gcccagctcc
     3001 tgcgcgaggg tcgtggcagc gggacgatct gcatcgtaaa ccgcggtgac atgaaatccc
     3061 ttttccttta agcggcgcgc catgtttgcg cccatccggc cgactcccac aaaagcgatg
     3121 ttcataaatc agatgcgacc agcaaaaaac ggattgtgct ttttttcctc tcccaccgtc
     3181 gtcatgggtc cgtggccagg gcagagaacg gtctcgtcgg gcaacgtgag aatttttttg
     3241 agattgttcc cgatcgcctc ttcgtaggaa acattgccgc cgcccatcga gcccgcaaag
     3301 agcgaatcgc ccacaatcgc gaccggccgc gccaagccgc tgatgacata ggtcatgcct
     3361 ccgggcgagt ggccccaggt caaccgcgct tcgatccgca ggcggccgag gtggaggatt
     3421 tttccttcct cgatcggttc ggcgccgggt gcctgctcgc gtgaagagag gaaaacgggc
     3481 gctccagtct cgcgcgtcag gcggcgcaaa tcggccacgt gatccggatg ggcatgggtg
     3541 agcaagagga gctgaatcgt taggccggcg cccgcgctgc gttggagcaa tggagaacaa
     3601 tccgcgccgc tatcgaaaac gaccgcctcc ctgctcgcgg gatcccagac gagataggcg
     3661 ttcaccgtca cgtcatgata gggggtgttg cactggaaca ggcccggaac ttcgatttcg
     3721 ttaggctgcc acttgttctg cgcgaggtcg gcaagagctc ccgggtggag ctggaggacg
     3781 ggcgcgattt ggtcgctcac cgcgcgatcg aatctcccct cccgtagctg gcgaatcgcc
     3841 acgacgctgg cccccgactt ttcagcgagc tgactgtcgg agattcccag accacgttgc
     3901 gctttgccca tgatgtcggt gaaattgtct tccagcggga tcgccatgta ccactaaaag
     3961 gcaaggagcg ttcgtgtcga gagggaagcg atgcgctctc tcgtcagctg cgctattcgc
     4021 gcggccatcc tttccgggga gtggaccctg ccggtttcct tttcgattga cgtcatc
//
LOCUS       DATMNS010000279         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3517546, whole genome shotgun sequence.
ACCESSION   DATMNS010000279 DATMNS010000000
VERSION     DATMNS010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3517546"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1052)
                     /locus_tag="VNW28_09765"
     CDS             complement(90..1052)
                     /locus_tag="VNW28_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXA10259.1"
                     /translation="MHLFLKVSRAVLMVSITLAPFQLPGVAQGKEHPVKYPTLYRTIQ
                     VDGLPIFYREAGPKDAPALLLLHGLPSSSRMFEPLFSRLSDRYHLVAPDYPGFGHSDW
                     PDPKTFAYTFDHIAETMNHFTEALGLSRYTLYMQDYGGPVGFRMALAHPDRIEALIVQ
                     DAVSHNEGLGANWKTRRAFWADRAANESALRGNLLSLATTRTRHVGSDPHVERYDPDL
                     WTDEFAFLNQPGQAEIQSDLFYDYRTNVDAYPTWQAWMRQKQPRLLVIWGKYDLSFEL
                     SEPEAYRRDVPNAEVRVFDAGHFALDTKAEGIAALVDGFMKARK"
     gene            complement(1066..1809)
                     /locus_tag="VNW28_09770"
     CDS             complement(1066..1809)
                     /locus_tag="VNW28_09770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017397564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXA10260.1"
                     /translation="MSNITDKVVVITGASSGIGEGTAKLLAQQGARVVLGARRSNRID
                     AVVKEISAAGGKAIGFATDVTKRAEVEALIKRAVDSFGRVDVLLNNAGIMPIAPIEAL
                     KVDEWDRQIDVNIKGLLYGVAAALPHMQKQKSGHIINITSVFGIKVFAPGGTVYCATK
                     AAGRALTEGLRMELHSQNIRCTMISPGAVATELAESSSDEATRKNLREFMKMAIPADS
                     IARAIAYAIEQPANVEIDEIVVRPIAQDF"
     gene            complement(1827..>2208)
                     /locus_tag="VNW28_09775"
     CDS             complement(1827..>2208)
                     /locus_tag="VNW28_09775"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HXA10261.1"
                     /translation="FLACEESGASPLHRQVLRGKRAGHTALTKGFTGRLARGIHNRFL
                     EELNQAGTAILPYPLQRRLVRNLAIPAEAAGRSDLLPLWAGQSANLSTCTDVSAFLTS
                     LVKKVSEIAGPHIQWIAALREANS"
BASE COUNT          439 a          651 c          631 g          487 t
ORIGIN      
        1 aaggttcgcc gacaccagca tccgaactgc ctgtagtgcg agtcttttgc ccatgaactt
       61 ttccccttga cctcctggaa ccggagtgtt catttccgcg ccttcatgaa cccatctaca
      121 agtgccgcaa ttccctccgc cttggtatca agagcgaagt gtcctgcgtc gaaaacgcga
      181 acctcagcgt tcggtacgtc gcggcgatag gcctcgggct ccgagagttc aaacgagagg
      241 tcatacttgc cccaaatcac cagcagccgc ggctgtttct gtcgcatcca cgcctgccac
      301 gtcgggtagg catcgacgtt tgtgcggtag tcgtagaaga ggtcgctttg aatttcggcc
      361 tggcccggtt ggttcaaaaa agcgaattca tccgtccaga gatccgggtc atagcgttcc
      421 acgtgggggt cgctcccgac gtggcgcgtc cgcgttgtgg ccagcgacaa cagattgcct
      481 cgaagtgcac tttcgttggc ggcgcgatcg gcccaaaagg cccgccgcgt cttccaattc
      541 gcccccagac cttcgttgtg cgaaacggcg tcctgaacga ttagagcctc gatccgatcc
      601 ggatgcgcca atgccatgcg aaagcctacg ggaccgccgt aatcctgcat gtagagcgtg
      661 tagcgcgata gcccgagcgc ttccgtgaag tgattcatcg tttcggcgat gtgatcgaac
      721 gtgtacgcaa atgtcttcgg gtccggccag tcgctgtgcc cgaagcccgg atagtcgggc
      781 gctaccaggt gatagcggtc ggaaagccga gagaacaggg gctcgaacat tcgggatgag
      841 gaaggaagcc cgtgcaacag caaaagcgcc ggtgcgtctt tcggcccggc ctccctgtaa
      901 aagatgggaa gtccatctac ctgtatcgtg cgataaagag tcggatattt cactggatgt
      961 tcctttccct gggcgacgcc cggcagttga aaaggtgcga gcgtaataga aaccatcagc
     1021 accgcacgag aaaccttcag aaagaggtgc accgtgtgcc tcgcgttaga agtcctgcgc
     1081 gatgggccgg acgacaatct cgtcgatctc tacgttggcg ggctgttcga tggcgtaagc
     1141 gatggcgcga gcgatcgagt cggcaggaat cgccattttc ataaactcgc gcagattctt
     1201 tctggttgcc tcgtccgaac tgctctccgc gagttccgtg gcgacggcgc ctggagagat
     1261 catcgtgcac cggatatttt gggagtgtaa ttccatccgg agcccttcag tcaaggcgcg
     1321 gcccgcggcc ttggtcgcgc aatataccgt tccacccgga gcgaacactt tgatcccaaa
     1381 aacggaagtg atgttgatga tgtgaccgct cttttgcttc tgcatgtgag gcagggcggc
     1441 ggcaactcca tagagaagcc ctttgatatt gacgtcaatc tgccggtccc actcgtccac
     1501 cttaagggcc tcgatcggcg cgatgggcat aatacccgcg ttattaagga gcacatcgac
     1561 gcgaccgaag ctgtcgacgg cccgtttgat cagcgcttcc acttctgcgc gcttcgtgac
     1621 atcggtggca aatccgatgg ccttgccgcc ggcggccgag atctccttaa ccactgcgtc
     1681 gatcctgttc gaacgccgcg ctcccagtac aactcttgct ccctgttgcg cgagaagctt
     1741 cgcggtgcct tccccaatgc cgctgctcgc gccggtgatg actacaactt tgtcggttat
     1801 atttgacata ttcgttttct ccttatttaa gaatttgctt cacggagggc cgcgatccac
     1861 tgaatgtgcg gccccgcgat ttcagaaacc tttttgacca atgaggtcaa gaaagccgat
     1921 acgtcggtgc aggtagaaag attggcgctt tgcccagccc ataggggcag aagatccgat
     1981 ctgcccgccg cctctgccgg gatcgcgagg tttcggacga gccttcgctg caacggataa
     2041 ggtagtatcg ccgtccccgc ctggttcaac tcctccagga aacggttgtg aattccccgc
     2101 gcgagtctcc cggtgaagcc ctttgtcagc gcggtgtgcc cggctctttt ccctcgcaag
     2161 acttggcgat gaagggggct ggcgccagac tcctcgcacg ccaggaat
//
LOCUS       DATMNS010000280         3671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2090190, whole genome shotgun sequence.
ACCESSION   DATMNS010000280 DATMNS010000000
VERSION     DATMNS010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090190"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1775)
                     /locus_tag="VNW28_09780"
     CDS             complement(<1..1775)
                     /locus_tag="VNW28_09780"
                     /inference="COORDINATES: protein motif:HMM:NF014598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HXA10262.1"
                     /translation="MIRLLKFLLWFGIALLGAGAVATAAFARGEPVNALWIVTAGLCA
                     MAISYRFYSKWLAAKVLVLNDERATPALVVDDGKDFVPTNRWMAFGHHFAAIAGPGPL
                     VGPVLAAQFGYLPGMLWILIGATLGGGVHDMIVLFASVRRQGKTLGQMVKEEIGSAVG
                     TLALISVLAIMVILLAVLALVVVQALAKSPWGVFTIAMTIPVAVIMGISLRGGRVGVG
                     WVTVFGLSGLLFSVWGGQFLAQYPALESWFRFDQKSLAWALMFYGFAASALPVWLLLT
                     PRDYLSTFLKLGTVAALAVAVIVIHPTLQMPALTRFIDGTGLVFAGPVFPFVCITIAC
                     GAVSGFHSLIASGTTPKMIESETRIRDIGYGAMVTEMMVALMALVAASVLPPGQYFAI
                     NAKGAPGEVVATVSAAGFPVTEPEMKQLATELGESTMFNRAGGAPTFAVGMAHMFSRI
                     TASPTALALWYHFAIMFEALFILTTLDAGTRVGRFLLQDLLGQFWHPLGDTGSWSANI
                     VTSALLVAAWGWFLYQGVLDPLGGINSLWPLFGLANQLLSVIALCLGTTILIKMGKAR
                     YLWTTLGPLAFMIVVTFSAGYLKIWS"
     gene            complement(1876..>3671)
                     /locus_tag="VNW28_09785"
     CDS             complement(1876..>3671)
                     /locus_tag="VNW28_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXA10263.1"
                     /translation="IGQRHGLPVIDVLHPNGTINCPDVPELNGLDRFAARKKAAEMLA
                     QSGLLAKEEPHESTVGFSERAGVPIEPRLSEQWFLKYPKVKEARAAVGSAIHFRPERW
                     EAVYAHWLQNIQDWCISRQIWWGHRIPVWYGKADPEKIHVALEPPPDLEKWTQDPDVL
                     DTWFSSWLWPFETMDAPTRAKFYPTNDLVTAPDIIFFWVARMIMAGLEYTGDIPFHDV
                     FFTGLIRDGQGRKMSKSLGNSPDPLDLIAKYGADGVRFGLMRIAPSGQDIRFDEKQIE
                     EGRNFANKLWNATRFRQMHGGEIEGEINPALLTSDDKWILLRLDAAIRAVTRALEQYN
                     FSEAAQALYRFFWSEYCDWYLEASKAALGTPDAEPPSARKANTLAVIDFALGHTLRLF
                     HPFLPFITEELWHGLGFNADLPDDQGAKTIQFARWPAPLDDEFKKHYGLTPNDEQSAN
                     AKYETVVAGRGLRRDFHIGSNKRVAFVLKPSGPLPEEEAAVLRILLNAERLDLATDYK
                     PTKGTPTALTPLGELYLPLEGLIDPDAERERLSKEVAKTEAELVTVRKKLANENFVAN
                     APTAVVAKHRQREKDFAERLEQLRRMLDSLG"
BASE COUNT          758 a         1152 c         1105 g          656 t
ORIGIN      
        1 gaccagatct tgagataacc ggcggaaaac gtgaccacga tcatgaaggc gagcggcccg
       61 agcgtggtcc agaggtagcg cgctttgccc atcttgatca ggatcgttgt cccgaggcaa
      121 agcgcgatga ccgagagaag ctgattggcc aggccgaaga gcggccagag actgttgatc
      181 ccgcctaacg gatcgagcac accctggtag aggaaccagc cccaagctgc gacaagcagc
      241 gcgctcgtga caatattcgc gctccacgac ccggtgtcgc cgagcgggtg ccagaattgg
      301 cccagcaaat cctgcaggag aaaacgcccc acccgcgttc cggcgtcgag tgtggtgaga
      361 ataaagagcg cctcgaacat gatcgcgaag tgataccaga gcgcgagcgc ggtcgggctg
      421 gctgtgatcc gtgaaaacat gtgcgccatt ccaaccgcaa aagtgggcgc cccgcccgcg
      481 cggttgaaca tcgtgctctc gcccagttcg gtcgcgagct gcttcatctc cggttcggtc
      541 acggggaatc ccgctgccga aacggtcgcg accacttcgc cgggcgcacc cttggcgttg
      601 atcgcgaagt attgcccggg gggaaggacg gaggccgcga ccagcgccat cagggcgacc
      661 atcatctcgg tcaccattgc gccgtagccg atatcgcgaa tgcgcgtttc actctcgatc
      721 attttcggcg tcgtcccaga cgcgatcagc gagtgaaatc cggagaccgc gccgcaggcg
      781 atggtgatgc agacgaaggg aaataccgga ccggcaaaga ccagccccgt gccgtcgatg
      841 aaacgcgtga gggccggcat ctggagggtg ggatggatga caatcaccgc cacggcgagc
      901 gcggcgaccg tgcccaactt gagaaaagta ctgagataat cacgcggggt gaggagcagc
      961 cagaccggca gcgcggaggc ggcgaagccg tagaacatga gcgcccaggc gagcgatttc
     1021 tggtcaaaac ggaaccagct ttcgagcgcc ggatattgcg cgagaaactg gccgccccag
     1081 acggaaaaaa gcaagccgga caacccaaaa actgtcaccc agccgacgcc gacccggccg
     1141 ccgcgcaagc taatccccat gatgaccgca accgggatgg tcatcgcaat cgtgaaaacg
     1201 ccccacgggc tcttggccag cgcctgcacc acgacgagag caaggaccgc gagcagaatc
     1261 accatgattg cgaggacgct gataagggca agcgtgccga ccgcgctccc gatttcctcc
     1321 ttcaccatct ggcctaacgt cttgccctgt cggcgcacgg aagcgaagag cacgatcatg
     1381 tcgtgcacgc cgccgcccag cgtggccccg atcaaaatcc aaagcatccc tgggagatac
     1441 ccgaattgcg ccgccagcac cggccccaca agcggaccgg gcccggcgat cgcggcgaag
     1501 tgatgaccaa aagccatcca gcgattggtc ggaacgaaat ccttgccgtc gtccaccacg
     1561 agcgcgggcg tggcgcgctc gtcattcagg accagcactt tggccgcgag ccacttgctg
     1621 tagaagcggt agctgatggc catggcacac aaacctgccg tcacaatcca gagcgcgttg
     1681 accggttcgc cccgggcgaa agcggcggtc gcgacggccc cggcgccgag gagtgcgatc
     1741 ccgaaccaga gaaggaattt cagaagtcga atcatgtcag gagagcgatt atagcgcgac
     1801 cggcgttgcg ccggcaatta cggagcgacg tgcttccgca cgtccatcga cgagcagaag
     1861 ctcgtccctc cgatcctacc ctaacgaatc gagcatccgc cggagctgtt cgagccgctc
     1921 cgcgaaatct ttttcccgct gtcgatgttt ggccacgacc gcggtcgggg cgttggcgac
     1981 aaaattttcg ttcgccagtt tcttccggac ggtcacgagt tccgcttccg ttttcgcgac
     2041 ctccttgctg aggcgttcgc gttccgcgtc gggatcgatc aagccctcga gcggcagata
     2101 aagctctccc aggggcgtca acgcggtggg agtgcccttg gtcggtttgt aatcggtggc
     2161 gagatcgagt cgctccgcat tgagcaggat gcgcaggacc gccgcctctt cctccggcaa
     2221 cggacctgac ggcttgagca caaacgcgac ccgtttgttc gacccaatgt ggaaatcccg
     2281 gcgcaagccg cggcccgcaa cgaccgtttc gtatttcgca ttcgcgcttt gctcgtcgtt
     2341 aggcgtgagg ccgtaatgtt tcttgaactc gtcgtccagt ggagccggcc agcgcgcgaa
     2401 ctgaatcgtt ttcgccccct ggtcgtccgg caggtcggcg ttaaagccga gcccatgcca
     2461 gagttcttcg gtgataaaag gcagaaacgg atgaaacaaa cggagggtgt gaccgagggc
     2521 aaaatcgatc accgccaagg tgttggcctt ccgcgcgctc ggcggttccg cgtccggtgt
     2581 cccgagcgcg gccttgctcg cttccaggta ccaatcgcaa tattcgctcc agaaaaagcg
     2641 atagagcgcc tgcgcggctt cgctgaagtt gtattgctcg agcgcccgcg taactgcgcg
     2701 aattgccgca tcgaggcgta acagaatcca cttgtcgtca ctcgtcagga gcgcgggatt
     2761 gatttcgccc tcaatctcgc cgccatgcat ctggcggaaa cgggtcgcgt tccagagctt
     2821 gttcgcgaag ttgcgacctt cctcaatctg tttctcgtcg aaacgaatgt cctggccgct
     2881 gggcgcgatc cgcatcaggc cgaagcgcac accatccgcg ccgtatttcg cgatcaggtc
     2941 gagcgggtcg ggcgagttgc ctaacgactt gctcatcttt cgtccctgcc cgtcgcggat
     3001 cagcccggtg aagaagacat cgtggaaagg aatatccccg gtgtattcga gccctgccat
     3061 gatcatccgc gcgacccaga aaaaaatgat gtccggcgcg gtcaccagat cgttcgtcgg
     3121 ataaaacttc gcgcgcgttg gtgcgtccat tgtctcgaaa ggccagagcc agctcgagaa
     3181 ccaggtgtcg agcacgtccg gatcctgagt ccacttttcc agatccggcg gcggctcgag
     3241 ggcgacgtgg attttctcgg gatcggcttt gccgtaccag accggaatgc gatgacccca
     3301 ccaaatctgg cggctgatgc accaatcctg aatgttctgc aaccaatgcg cgtagaccgc
     3361 ttcccagcgc tccgggcgga aatgaatcgc gctccccacg gccgcgcggg cttccttcac
     3421 tttcggatat ttcaaaaacc attgttcgct cagacgcggc tcgatcggca cgccggcacg
     3481 ttcgctaaag ccgactgtgc tctcatgcgg ttcttctttc gcaagaagac cgctttgcgc
     3541 gagcatctca gccgccttct tccgcgccgc gaaacggtcc agcccgttga gctcaggcac
     3601 gtcgggacaa ttgatcgttc cattcgggtg caggacgtcg atcaccggca ggccatgccg
     3661 ctggccgatc t
//
LOCUS       DATMNS010000281         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4168129, whole genome shotgun sequence.
ACCESSION   DATMNS010000281 DATMNS010000000
VERSION     DATMNS010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4168129"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VNW28_09790"
     CDS             complement(<1..620)
                     /locus_tag="VNW28_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10264.1"
                     /translation="MKISFSILFRRPQLAAVAVSLGLLGLSVHAAPLGAPATASGVLA
                     TPPGKTAPVAALQDDIFDIRPPIHLPGEIFWTGWAAGAVAAAALGYGAWRLLRGKLHA
                     KLPYEVALEKLLAAHQFLSPEEARAFSDLVSEVVRSFIEQTFPVRAAHRTTEEFLSDL
                     VVHTDSPLAGHRGTLGEFLGHCDLAKFARWQLSVPQMEAMLQSARD"
     gene            complement(617..1528)
                     /locus_tag="VNW28_09795"
     CDS             complement(617..1528)
                     /locus_tag="VNW28_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXA10265.1"
                     /translation="MTSEQTRDILRKVRQIEIRTKRLVTDTLAGQYHSVFKGRGMNFD
                     AVREYSPGDEVRTIDWNVTARAGRPFVKKFVEERELTLTLMVDVSASGEFGSGAQSKR
                     ELAAEIASVLAFSAVHNSDKVGLILFTDQIELYVPPRKGRRHVLRLVREVLGFEPQHR
                     GTNLVRALDFVNDVIHRRAIVFLLSDFQTGRQTPESVADLRRALRQTNLRHDLVAMPI
                     EDAREQVLPDVGILALEDAETGELIELNTSLPAVRRRFAEIAQNRAKQLQRTLNAEAV
                     DALRLSTDQPYVASLISFFKNRSRKHR"
     gene            complement(1560..>2054)
                     /locus_tag="VNW28_09800"
     CDS             complement(1560..>2054)
                     /locus_tag="VNW28_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXA10266.1"
                     /translation="TYPLPEAQVDRFMLKVLVKYPNRSEERAILDTMAVTERSFEVQP
                     VVSAEDILQARKVVSSIYVDEKVKDYVVDIVLATRDPQPYGIDLNGYVQCGASPRATI
                     SLTLGARAIAFLQGRGYVTPQDVKDVAYDVLRHRIIVSYEAEAENIASENIIAGILEG
                     LPVP"
BASE COUNT          392 a          597 c          668 g          397 t
ORIGIN      
        1 aagtcgcgag cgctttgcag catggcttcc atttgcggga cgctcagctg ccacctcgcg
       61 aacttcgcga gatcgcagtg gccgagaaat tcgccgagcg ttccccgatg ccctgcgagg
      121 ggactgtcgg tgtgaacgac cagatcgctc agaaactcct cggtcgttcg atgtgcggcg
      181 cgcaccggaa acgtttgctc gatgaaggag cgaacgacct ctgagactag gtcggagaag
      241 gcgcgcgcct cctccgggct cagaaactgg tgggcggcca ggagtttttc gagcgcgact
      301 tcgtaaggca gcttcgcgtg caactttccg cgcaagaggc gccaggcgcc gtaaccaagc
      361 gccgcagcgg cgaccgcgcc ggcagcccaa ccggtccaga aaatttcacc gggaaggtgg
      421 atcggcggcc ggatgtcgaa gatgtcgtcc tgcaacgcgg cgaccggagc agtcttccct
      481 ggtggcgtgg ccaatacgcc ggaagcggtc gccggcgcgc ccagaggcgc cgcgtggacg
      541 ctcagcccga ggaggccgag cgaaacggcg actgctgcca gctgcgggcg gcggaaaaga
      601 atcgaaaacg aaattttcat cgatgtttgc gggatcggtt tttgaaaaag gagatcagcg
      661 atgccacgta gggctgatca gtggagaggc ggagcgcgtc gacggcttcg gcatttaagg
      721 tgcgttggag ttgcttcgca cggttttgtg cgatctccgc gaaacgccgc cgcaccgcgg
      781 gcaagctcgt gttgagctcg ataagttccc cggtctcggc gtcctcgaga gcgagaatgc
      841 cgacgtcggg tagcacctgc tcacgggcgt cttcgatcgg catggccacc aggtcgtgac
      901 gaagattagt ctggcgaagg gcgcggcgaa gatcagccac tgactcgggg gtttgccggc
      961 cggtctggaa gtcggacagc aggaagacaa tcgcgcgcct gtggataacg tcgttcacga
     1021 aatcgagcgc ccggacgaga ttggtaccgc ggtgttgcgg ttcaaagcca agtacctcgc
     1081 gcaccaatcg gagcacgtgt cgccggcctt tgcgcggcgg cacgtagagc tcgatctggt
     1141 cggtaaagag aatcagtccg accttgtcgc tattatgcac cgcggagaag gccaggacgc
     1201 tggcgatctc ggccgcgagt tcgcgcttgc tctgcgcgcc agacccgaac tcgcccgagg
     1261 cgctcacgtc caccatgagc gttaatgtga gctcgcgttc ctcgacaaat tttttgacga
     1321 atgggcgtcc ggctcgagcc gtcacgttcc agtcgatggt tcgcacttca tcgccaggcg
     1381 agtattcgcg cacggcgtcg aagttcatcc cgcggccttt gaaaacgctg tggtattggc
     1441 ccgccagcgt gtcggtcaca agccgcttgg tgcggatctc gatttgccgc actttgcgca
     1501 gaatatctct tgtttgttcg gaagtcatgg cgttgggcct cgatgcgcgc gcagcgcgat
     1561 taggggacag gcaatccttc caggatgccg gcgatgatat tttccgaggc gatgttctcc
     1621 gcctcggcct cgtagctgac gatgatccgg tgacgcaaaa catcgtaagc gacgtctttc
     1681 acatcctgcg gggtgacata gccgcggccc tggaggaacg cgatcgcacg ggccccgagg
     1741 gtcagactga tcgttgcgcg cggggacgcg ccgcattgca catacccatt gaggtcgatt
     1801 ccgtagggct ggggatcgcg cgtcgcgagg acaatgtcca ccacgtagtc cttaaccttt
     1861 tcatcgacgt agatgctgct gacaacctta cgcgcctgaa gaatgtcttc cgcggagaca
     1921 accggttgca cttcgaacga acgctctgtg acggccatgg tatcgaggat cgcgcgctct
     1981 tcgctacggt tcgggtattt caccagaacc ttcagcatga agcggtcgac ctgcgcttcg
     2041 ggcagcgggt aagt
//
LOCUS       DATMNS010000282         3226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3817188, whole genome shotgun sequence.
ACCESSION   DATMNS010000282 DATMNS010000000
VERSION     DATMNS010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3817188"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..788
                     /locus_tag="VNW28_09805"
     CDS             <1..788
                     /locus_tag="VNW28_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HXA10267.1"
                     /translation="SCVMAEQILAGKTGVVFGVANKRSIAWAIAKAWAAAGARLIFNY
                     QGERLKDNVEELVAEFGENTPLYPCDVSQDEEIASFFAKVSEQTERLDLLLHSVAFAP
                     KEALEGDFLSTTRDAFRTAHDISAYSLVALTRAATPFMTEGGSILAMTYYGAEKVVPH
                     YNVMGVAKASLEASTRYLAYDLGPKRIRVNCISAGPVNTLAARGISGFSAMLKHYQER
                     APLKRSCDPAELGQTGLFLASDGAAAITGQVLYVDGGYQIMGM"
     gene            complement(794..1132)
                     /locus_tag="VNW28_09810"
     CDS             complement(794..1132)
                     /locus_tag="VNW28_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HXA10268.1"
                     /translation="MTLFEKIIAREIPAQIVFEDEEVMAFHDVNPQAPMHVLIVPKRA
                     IPRLAQTSESDQALLGKLILTAAKVARELGVSESGYRLVINSGPDAGESVPHLHVHLL
                     GKRTLDWPPG"
     gene            complement(1129..1689)
                     /locus_tag="VNW28_09815"
     CDS             complement(1129..1689)
                     /locus_tag="VNW28_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10269.1"
                     /translation="MVAFKEEFPYLRSDSGQLFEFNRAWLHAAIKQAADRAGYPRWWL
                     TEHVTESITFYMRLRTDESVVAFSQLNQTVRYVLKVIGYNEIVPHFSPAPPPISVSLY
                     EIAEEAGAGYELAFFDLLEKRVAAIIETGATGLHFFGLQPTIKHLRGVKTWTRSCDAL
                     REEVTCFIREKICNGAEVAELNCSIR"
     gene            complement(1676..2185)
                     /gene="nrdR"
                     /locus_tag="VNW28_09820"
     CDS             complement(1676..2185)
                     /gene="nrdR"
                     /locus_tag="VNW28_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXA10270.1"
                     /translation="MRCPKCGNRDDKVIDSRQSRDSSSIRRRRVCLACAFRFTTYEEI
                     ERTDLRVIKRDRTHEAFDRRKLANSVAKACEKRSISLMTLEKTVNDIVHELETSGREI
                     SWAAIGDKVLEKLREVDEVAYLRYASVHRRFEDVERFVDEIHALGRRIKPDALQPELF
                     NEPANNGRV"
     gene            complement(2297..>3226)
                     /locus_tag="VNW28_09825"
     CDS             complement(2297..>3226)
                     /locus_tag="VNW28_09825"
                     /inference="COORDINATES: protein motif:HMM:NF024738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HXA10271.1"
                     /translation="VTDIISPTDGQPMVETLRGYKAQDGGLMIYKVMSGRKIEEEEVR
                     QLVADGEIGPVDGFVSAKTGNRFTSKIRIVEDEKNPGRKKAELDFGNRIDVNALVPFW
                     TDPKNGAELCEAATNYVLRERNGEDWKETFKVGRLMCQKEITREQAVQLVTRGKTDLI
                     GKFTSKKGRPFDAYLVRQGARISWEFPPRKPREGGEKNGKGAPRKVKPPLDLAKAVKL
                     GESKIHHGDLYQTEDAFVVAKSQTDGSPRVVFELKRNLCGKDISAEDAERLVELGKTG
                     LIEGFVSKRGSNFSAYLTLSKDKKKAEFEFPPR"
BASE COUNT          633 a          971 c          913 g          709 t
ORIGIN      
        1 agtcctgcgt tatggcagag caaattctcg cgggcaaaac gggcgtcgtc ttcggcgtcg
       61 ccaataaacg cagcatcgcg tgggcgatcg ccaaggcctg ggccgcggcc ggcgcgcggc
      121 tcattttcaa ttaccagggc gagcgcttga aagacaacgt cgaagaactc gtcgccgagt
      181 tcggagaaaa tacgccgctt tatccttgcg atgtttccca ggatgaggag atcgcttctt
      241 tttttgcgaa agttagcgag cagaccgagc ggcttgatct gcttctccat tcggtcgctt
      301 tcgcgcccaa ggaagcgctc gaaggcgatt tcctcagcac gactcgcgac gcgtttagaa
      361 ccgcacacga catcagcgcc tactcgttag tcgcgctcac ccgcgcggcg acgccgttca
      421 tgaccgaggg cggaagcatt ctcgcgatga cctattacgg cgcggagaag gtggtgccgc
      481 actacaacgt gatgggcgtc gcgaaggcga gcctcgaagc cagcacgcgc tacctcgcct
      541 atgatctcgg tccgaaaagg attcgcgtga attgcatctc cgccggcccg gtcaacaccc
      601 tcgccgcacg cggaatcagc ggtttttccg cgatgctgaa gcattatcag gaacgcgcgc
      661 cgctgaagcg gagttgcgat cccgctgagc ttggccagac cggcttgttc ctcgcgagcg
      721 acggcgccgc cgcgatcacc ggccaggtgc tctacgtgga cggcggttat cagatcatgg
      781 gaatgtgatt tcgttagccg ggcggccagt cgagcgtccg tttgcccaac aagtggacgt
      841 gcaggtgcgg cacgctctcg ccggcgtctg ggccgctgtt gatcacgagg cgataaccgc
      901 tctcgctgac cccgagctcg cgcgccactt tcgccgcggt caagatcagc ttcccaagca
      961 gtgcttgatc cgactcgctc gtctgagcga gccgcgggat ggcgcgcttg ggcacgatca
     1021 ggacgtgcat cggcgcctgc ggattgacgt cgtgaaaggc catcacctcc tcatcctcga
     1081 aaacgatctg cgcgggaatt tcacgcgcaa tgatcttctc gaagagggtc atcgaatcga
     1141 gcaattcaat tccgccacct ccgcgccgtt gcaaatcttt tcgcggatga agcaggtcac
     1201 ttcctcacgc aacgcgtcgc aggagcgggt ccaggttttg actccgcgca gatgtttgat
     1261 cgtgggttgc aaaccaaaaa aatgcaaccc agtcgccccg gtctcgatga tcgcggcgac
     1321 tcttttttcg agtagatcga aaaaggccag ctcgtaaccc gcgcccgcct cttccgcgat
     1381 ctcgtaaagc gaaacggaaa tcggcggcgg cgcgggactg aaatgcggca cgatttcgtt
     1441 gtagccgatg actttcagga cgtagcgcac cgtctggttg agctggctga acgcgacgac
     1501 cgattcatcg gtccgcagcc gcatatagaa ggtaatgctt tccgtgacat gttcggtcag
     1561 ccaccagcgc ggataaccgg cgcgatcggc cgcctgcttg atcgcggcat gcaaccaagc
     1621 gcgattgaac tcgaaaagct gcccggaatc ggagcggaga tagggaaatt cttctttaaa
     1681 cgcgaccatt gtttgcgggt tcgttaaaca actcgggttg gagcgcgtcc ggcttgatcc
     1741 gccggccgag ggcatggatt tcatcgacga aacgctcgac atcctcgaag cgccgatgaa
     1801 ccgaggcgta acggaggtag gccacctcat cgacttcgcg cagcttttcc agcaccttgt
     1861 cgccaattgc ggcccatgaa atttcgcgcc cgctcgtctc cagttcatgc acgatatcgt
     1921 tgactgtttt ctcgagcgtc atcaggctga tggaacgttt ttcgcaggcc ttcgccacgc
     1981 tgttcgccag cttgcggcga tcgaaggctt catgcgtgcg gtcccgttta atcacgcgca
     2041 gatccgtccg ttcaatttct tcgtaagtgg tgaaacggaa agcacaggca aggcaaactc
     2101 gccgccggcg gatgctggaa ctatctcgcg attgtcgcga atcgatgacc ttgtcatcgc
     2161 gatttccgca ctttggacag cgcatggtgt ggtcgggaga acaaactcca ctacacgtgg
     2221 tagcacggag gatggcaaga gatttcgacg cggtcgtcga aaaaaatttg ccgcgacaat
     2281 ttttcgcggc tcgactctag cgcggcggaa actcgaactc ggctttcttc ttgtccttcg
     2341 aaagcgtgag gtaggcgctg aagttcgaac cgcgttttga aacaaagcct tcgatcaaac
     2401 ccgtcttgcc caactccacc aaccgctctg catcctcggc ggaaatatct ttgccgcaga
     2461 gattgcgttt caattcgaag acgacccgcg gcgaaccgtc ggtctgagat ttcgcgacga
     2521 cgaaggcgtc ttccgtttgg tagagatcgc cgtggtggat cttgctttcg cccagcttca
     2581 cagccttcgc caagtcgagc ggcggtttta ccttgcgtgg tgcgcccttg ccgtttttct
     2641 cgccgccctc acgcggtttg cgcggaggaa attcccagct gatccgggct ccttgcctaa
     2701 cgagataggc gtcgaagggc cggcccttct tcgaagtgaa ctttccgatc agatcggttt
     2761 tcccgcgggt aacgagctga acagcctgct cgcgcgtgat ttccttctgg cacatcaggc
     2821 ggccgacctt gaaagtttcc ttccagtcct ctccattccg ctcgcgcaag acgtagttgg
     2881 tcgccgcttc gcaaagttcc gcgccgtttt tcggatcggt ccagaacgga acgagcgcgt
     2941 tgacatcgat cctgttaccg aaatccagct ccgccttttt gcggccggga ttcttctcat
     3001 cctccacgat cctgattttc gaggtaaaac gattgcccgt ttttgcggaa acaaatccat
     3061 cgaccggacc gatctcgccg tcggccacga gctggcgaac ttcttcttcc tcgattttcc
     3121 gcccgctcat caccttgtaa atcatcagcc cgccgtcctg cgctttgtaa ccgcgcaggg
     3181 tttcgaccat cggctggcca tcggtcggcg aaatgatatc ggtgac
//
LOCUS       DATMNS010000283         5141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3159147, whole genome shotgun sequence.
ACCESSION   DATMNS010000283 DATMNS010000000
VERSION     DATMNS010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3159147"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VNW28_09830"
     CDS             complement(<1..253)
                     /locus_tag="VNW28_09830"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HXA10272.1"
                     /translation="MPPISTAERTLRFLGMPLARLIYRVTAVGRERLPSGGFLLLPNH
                     ITWVDAIVLLLACPRPIRFIIDQEYYENRFLHPILRTVGC"
     gene            303..1565
                     /locus_tag="VNW28_09835"
     CDS             303..1565
                     /locus_tag="VNW28_09835"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXA10273.1"
                     /translation="MKSESQIKPGAYVALALLLGINLFNYVDRYILAAVEPEIRATFF
                     AADDRNAMAVNGLLGTAFFVTYMLSAPALGWLSDRFSRWLIIGSAVLLWSLASGASGL
                     AATFSLLVFTRILVGIGEGGYGPAGPTILADLFPRAIRGRILSIFCAAIPVGGALGYV
                     LGSVINTHLGWRWAFYLVTPPGLLLGLLCFTQRDPRARETERKARDRATWDDFLTLLR
                     THSYVINCFAQTAMTFAFGGLAFWMSAYLRYRSEPASATAIFGAITALAGLLSTLTGG
                     VVADRLQRHYAGSYFLVSGIGMLIAAPLFVAMLFTPFPAAWGFLFGAVFFGFLNTGPS
                     NTALANVAFPNVRATAFALNILVIHALGDALAFPAIGYISGHSTMNIGFLVISGMMLI
                     AGVIWLAGIKSLGPDTLRVEGVPAPSPL"
     gene            complement(1645..3450)
                     /locus_tag="VNW28_09840"
     CDS             complement(1645..3450)
                     /locus_tag="VNW28_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXA10274.1"
                     /translation="MKKKVAPFLLLLLALATYAPALRDGFVWDDQALILADPLIRSWR
                     LIWEGFQHFLFTDAAASDFYRPLQRLSFTVDYAAFFLSPAGYHLVSILWHSAAAIALF
                     YFAEEFLSLGKMEPGRRFTVAFLAALVWMLHPVQSAAVIYVAGRADPLAAAFGFLALF
                     LALRMLRADGNRKWALGIGAGLAFLGSTLSKEMGLVFLFVWLIIALTQRRRAVSLGAI
                     GLIAGVLVAYLSLRLPAEHISPPPPRSIPFLVRPIVVARAAAEYAGLLIFPWDLHMER
                     DVETHPTGFDAASMNAASWRELQTLLGLILIAAAIYAFWRARRQPAIFLLLLLAVACY
                     LPVSGVIALNATVAEHWLYLPSAFLFLAAAVALDSSGWIAGQSLLSRGLTVGLSIWLL
                     FLPLRTFARTFDWKDQRTFLTRTIADGGDSARMLINLGGLELSEGHLEAARQALEKAL
                     VMEPDSPLARLNLGAVAIKERDFPKARTLLKALTDPPEIRARAEESLAVLENRESGQV
                     DLQRLRLAARIGPANWEIEKRYIKALADFSFPDRALTELKTCLATAPYRAETWQMTSE
                     LLHKNGRPNEAAAALAEAEADDVHLHDRISDNTPR"
     gene            3500..4327
                     /locus_tag="VNW28_09845"
     CDS             3500..4327
                     /locus_tag="VNW28_09845"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXA10275.1"
                     /translation="MFFFPRFVLGLALLSMASSAHAAGVPGDCEQLIVGLAPGWNSMR
                     GELERFERSASGPWRRVGGPTPALFGKNGLAWGRGLAGQDESGLRKRERDGRAPAGVF
                     RIGTIYTYDPALPAGADFPFHQVTKADAWIDDPTRPDYNRFVTIDDPEHPPAWFAREK
                     MRHSDFAYRWLVEVRHNSDPPLPNEGSAIFFHIRRGSARPTSGCTTMAEPDLVQLITW
                     LRAERHPCYALLPRTVYDAKAEAWKLPAPGLFGGAENFKIPASDICITEKLTLRLTR"
     gene            4338..4661
                     /locus_tag="VNW28_09850"
     CDS             4338..4661
                     /locus_tag="VNW28_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10276.1"
                     /translation="MRKRSNPDSEQNIKRIDTKPRAKKQTHGFQVHFVRGASTGTRMF
                     SDAVYGGKEPARKAARKYRREVMGDLPPRTTLASAKRRSTSKAKAKAKKSSAKKPVKK
                     GKKKR"
     gene            complement(4658..>5141)
                     /locus_tag="VNW28_09855"
     CDS             complement(4658..>5141)
                     /locus_tag="VNW28_09855"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXA10277.1"
                     /translation="VTGAAPRGEVHGNNLRHRAIHIFLFNCAGELLLQKRSRWKDRHP
                     GLWDSSAAGHVNAGEEYDATATRELFEELGVTTKLERVAKLAASERTGQEFIWLYRGE
                     HEGPFVPARAEIDSVQFFPRDAVARWIDRRPGDFAPGFIECWRADRSRNERASQTNPA
                     "
BASE COUNT          978 a         1603 c         1604 g          956 t
ORIGIN      
        1 tgcaaccaac cgtgcgcagg atcggatgga ggaagcgatt ttcgtaatat tcctggtcga
       61 tgatgaaacg gatcggacgg gggcaagcga gcaggaggac gatcgcatcc acccaggtga
      121 tgtggttggg caaaagcaag aatccaccgg acggaaggcg ctccctgccg accgccgtga
      181 cgcgataaat cagacgcgcg agcggcattc caagaaaacg cagggtccgc tctgcggtgg
      241 aaatgggtgg cattgcttta tggtttacaa tctgcgcgtc gcctactgca atcggcaatt
      301 cgatgaagag tgaatcgcaa atcaagcccg gagcctatgt cgccctcgcg ttgctcctcg
      361 ggatcaatct ctttaattac gtcgaccgtt acatcctcgc cgcggtcgag ccggaaatcc
      421 gcgcgacttt ctttgcggcc gacgatcgca atgcgatggc ggtcaacgga ttgttaggca
      481 cggctttttt tgtcacctac atgctctccg ctccggcgct cggctggctt tcggaccgtt
      541 tttcccgctg gctgattatt ggctcggccg ttcttctctg gagtctggcg agcggcgcct
      601 cgggcctcgc ggcgacgttt agccttctcg tcttcacccg gattttggtt gggatcggcg
      661 agggcggtta cgggccggcc gggccgacca ttctggcaga tctctttccg cgcgcgatcc
      721 gcggtcgcat tctctcgatt ttctgcgcgg ctatcccagt gggtggcgct ctcggctacg
      781 tgctcggaag tgtgatcaac acccatctcg gttggcgttg ggccttttac ctggtcaccc
      841 cgcccgggtt gcttctcggt ttgctctgct tcacgcagcg cgacccgcgc gcgcgagaga
      901 ccgagcgcaa agcgcgggac cgcgccacct gggatgactt tctcaccctc ttgcgcacgc
      961 attcctacgt catcaattgt ttcgcgcaga ccgccatgac ttttgccttt ggcggcctcg
     1021 ctttttggat gtctgcttat ctgcgatatc gcagcgagcc cgcgtcagcg accgccatct
     1081 tcggcgcgat caccgcgctc gccgggctgc tctcgacgtt aaccggcggc gtagtcgccg
     1141 atcgtctgca acgacattat gccggatcct atttcctcgt ctccggaatc ggcatgctga
     1201 tcgccgcgcc gctgtttgtg gcgatgcttt tcactccgtt ccccgcggcc tgggggtttc
     1261 tcttcggcgc ggtcttcttt ggttttctca acaccggtcc ctccaatacg gctctcgcca
     1321 acgtggcttt cccgaacgtt cgcgcgacag ctttcgcgct gaatatcctc gtcatccacg
     1381 ccttgggcga tgcgctcgcc tttcccgcga ttggctacat ctcgggtcac tcgacgatga
     1441 atatcggttt cctcgtcatc tcgggaatga tgctcattgc cggggtcatc tggctggccg
     1501 ggataaaatc cctcggcccc gacacgctgc gcgtggaagg ggtgccggct ccgtcgccgc
     1561 tctagattcg ttccttcgcg ggaggctgga cgcgcggaag cgcgtcgctc tgccgcgcgg
     1621 tctcgctgct tctgatccac cgcctcagcg aggcgtgtta tccgaaatcc gatcgtgcag
     1681 gtgcacgtca tctgcttcgg cttcggccaa cgcggcggcg gcctcgttag gccgtccgtt
     1741 cttgtgcagg agttcgctcg tcatctgcca ggtctccgcg cgatagggcg cggtggcgag
     1801 gcaggttttc aattccgtta gcgcgcggtc gggaaaacta aagtcggcca acgccttgat
     1861 gtaacgcttc tcgatctccc aattcgcggg cccaatccgc gccgccaggc gcaaccgttg
     1921 caaatccacc tggccgctct cccggttttc gagcacggcc agactttcct cggcacgtgc
     1981 ccggatctcc ggcgggtcgg tcagcgcttt gaggagcgtt cgcgccttgg gaaaatcgcg
     2041 ttctttaatg gcgacggcgc caaggttgag ccgggcgagc ggactgtcgg gttccattac
     2101 cagcgctttc tccagtgcct ggcgcgcggc ctcgagatga ccttcgctca gttccaatcc
     2161 gccgagattg atcagcatcc gggcggagtc gccgccgtcc gcgatcgtcc gggtcagaaa
     2221 cgtgcgctga tctttccaat cgaaggtccg ggcaaaagtg cggagcggaa gaaaaagcaa
     2281 ccagatcgac aggccgacgg tcagcccgcg ggagagcaga ctctggcccg cgatccagcc
     2341 tgacgaatcg agcgcgaccg cggcggcaag gaacaaaaat gcgctgggca ggtagagcca
     2401 gtgctcagcg accgtggcgt tgagggcgat cactccgctg accggcaaat aacaggcgac
     2461 cgcgaggagc aggagaagga aaattgccgg ctgccggcgc gcgcgccaga aagcgtaaat
     2521 tgcggcggcg atgaggatga gaccgagcag cgtttgcagc tcgcgccaag aagctgcgtt
     2581 catgctcgcg gcgtcgaatc cggtcggatg tgtctcgacg tcgcgttcca tgtggagatc
     2641 ccatggaaag atcaggaggc cagcgtactc cgccgcggcc cgggccacaa cgatcggccg
     2701 cacgaggaat gggatcgagc gcggcggggg cggcgaaatg tgctcggcgg gcaggcgcaa
     2761 actcagatag gcaaccagga cgccggcaat gaggccgatt gcgcctaacg agaccgcgcg
     2821 gcggcgctgg gtgagggcga tgatcagcca gacgaacaga aagacgagcc ccatctcctt
     2881 gctcagcgtg ctcccgagaa aagcgagacc ggcgccgata ccaagggccc atttgcgatt
     2941 tccgtccgcg cgcagcatgc ggagggcgag gaagagcgcg aggaagccaa aggccgccgc
     3001 gagcggatcg gcccgacccg ccacgtagat gactgcggcg ctctgcaccg gatgcagcat
     3061 ccagacgagc gcggcaagga aagcgacggt gaagcggcgt ccgggctcca tcttgcccag
     3121 cgatagaaat tcctccgcaa aatagaagag cgcgatcgct gccgccgaat gccacaaaat
     3181 gctgacgagg tgatagccgg ccggcgacag aaagaaggca gcataatcaa ccgtgaagct
     3241 gagccgttgc agcggccgat agaaatccga ggcagccgcg tcggtgaaaa ggaagtgctg
     3301 aaatccctcc cagatcagtc gccagctgcg gatcaatgga tcagccagga tgagcgcctg
     3361 gtcgtcccaa acgaatccgt cgcgcaacgc cggcgcatac gtagccagtg ccagcaggag
     3421 gagcaggaac ggcgcgactt tcttcttcat ctcgaattca actggacaga tcgaatcgcc
     3481 tgcgcgtgta agtttggaaa tgtttttctt tcctcgcttt gtcctcgggc tcgcattgct
     3541 ctccatggcc agcagcgcgc atgccgcggg cgtcccgggc gattgcgagc aactgatcgt
     3601 cgggctcgcg ccgggttgga actcgatgcg gggcgagcta gagcgcttcg agcgttccgc
     3661 gagcggacca tggcgacgcg tcggcgggcc gacccccgcc ctcttcggca agaacggcct
     3721 cgcctggggc cgcggcctgg ctggccagga cgaaagcggc ttgcgcaaaa gagagcgcga
     3781 cggacgcgcg ccggccggag tttttcgcat cggcacgatt tacacctacg acccggcgct
     3841 cccggcgggc gcggattttc cctttcacca ggtgaccaaa gccgatgcct ggatcgacga
     3901 tccgacgcgg ccggattaca accgtttcgt cacgattgac gatccggagc atccaccggc
     3961 ctggttcgcc agggaaaaaa tgcggcatag cgacttcgcc taccggtggc tggtcgaggt
     4021 gcggcacaac tcggatccac cgctgcctaa cgaggggagc gcgatctttt ttcacatccg
     4081 acggggaagc gcgcgcccga cctccggctg caccacgatg gcggagccgg atctggtgca
     4141 gctgatcacc tggttgcgag cggagcgcca cccctgttac gcgctcctgc cccgaaccgt
     4201 ctacgatgcc aaagccgagg cctggaaatt gcctgcgccc ggactcttcg ggggggcgga
     4261 aaattttaaa attcctgcaa gcgacatttg cataaccgaa aagttgacct tacgcttaac
     4321 tcgttaggcc aaaaaatatg aggaaacgat ccaatcccga ctccgaacaa aacatcaagc
     4381 gcatcgacac caaaccacgc gccaagaaac agacgcacgg cttccaggtc catttcgtgc
     4441 gcggtgcatc gactggcacg agaatgttca gcgacgccgt ctacgggggc aaggaaccgg
     4501 cccgcaaagc ggcccgcaaa tatcgccgcg aagtgatggg cgacctgcct ccacggacca
     4561 cgctcgcgtc ggccaaaagg cgcagcacaa gcaaggcgaa ggctaaggcg aagaaatcca
     4621 gcgcgaagaa gcccgtcaaa aagggcaaga agaagcgtta ggctggattg gtttgcgagg
     4681 cgcgttcgtt ccgggagcgg tcggcgcgcc agcattcgat aaagccgggc gcaaaatcgc
     4741 ccggccgccg gtcgatccat cgcgcgaccg catcccgcgg gaagaactgc acgctgtcga
     4801 tctccgcgcg tgctgggacg aaaggtccct cgtgctcgcc tcggtagagc caaatgaatt
     4861 cctggccggt ccgctcggac gccgccagtt tcgcgacgcg ctccagcttg gtcgtcacgc
     4921 ccagctcttc gaagagctcg cgcgtggcgg tcgcgtcata ctcttcgccg gcgttgacgt
     4981 gcccggcggc gcttgagtcc cagaggccgg gatgccgatc cttccaacgc gaacgcttct
     5041 gcaaaagcag ttcgccagcg cagttgaaaa ggaagatgtg gatggcgcga tggcgcaagt
     5101 tattcccatg cacctcgccc cggggcgcag ccccggtcac a
//
LOCUS       DATMNS010000284         4494 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2093481, whole genome shotgun sequence.
ACCESSION   DATMNS010000284 DATMNS010000000
VERSION     DATMNS010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2093481"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VNW28_09860"
     CDS             complement(<1..633)
                     /locus_tag="VNW28_09860"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HXA10278.1"
                     /translation="MKAPGHRPFSLSRVAAITGNTFTELVRLKIFYFLLLFALLLIGN
                     SVFMASLSFQQEFQVLKDVSLGAMSIFTSLLAIVATAQLLPRDVEDRTIYTILAKPVR
                     RYEYLLGKLAGVLLLLALSLLAMSAVFLAVLFIREHILINATLRETAGLRPAQVEEAV
                     RGLRASAFNANLLPGIAIIYLKASLLASLTLFVSTFATSNIFTIVVMVCIY"
     gene            complement(630..1487)
                     /locus_tag="VNW28_09865"
     CDS             complement(630..1487)
                     /locus_tag="VNW28_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA10279.1"
                     /translation="MIEPAVAVQNLTKIFPSPFRRKKLIAVRGLNLEVAPGQVYGLLG
                     PNGSGKSTTLKIILGLISPTTGGSKIFGRDSREVRSREAVGFLPENPYFYKYLTGEET
                     LRFYGKLCGIRGQKLRTRTDELLALVGLQEARARRLGGYSKGMLQRIGLAQALIQEPK
                     LLVLDEPTAGVDPAGSSEIRDLILDLKQRGITVLLSSHLLAQVQEVCDRVGILAHGVL
                     VREGRVEDLLAIENQTELILENATPEILAQITAVLGKSSARLVAQRQSQTTLERLFLE
                     ATSAPPHQE"
     gene            complement(1480..1755)
                     /locus_tag="VNW28_09870"
     CDS             complement(1480..1755)
                     /locus_tag="VNW28_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HXA10280.1"
                     /translation="MISLQVFFEGNVQGVGFRWSIKHLAKGFDLVGWVRNLPDGRVEL
                     QISGEETEVRAFLEAIGQSELRGHIRKQTEFLPPEPPRAHGFEIRHD"
     gene            complement(1869..2327)
                     /locus_tag="VNW28_09875"
     CDS             complement(1869..2327)
                     /locus_tag="VNW28_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10281.1"
                     /translation="MKLRCLIPLGAVALLAVGCVSSIKLSALSGTTNTWYSVAANQTP
                     FYHYGPQQGSGPDSQLPKDSIMKVIRPSFGYVKVQLQDGESGYVASEDIRPAPAALVT
                     EKLAPPPTPTPTFAGQGEEFQLNSDDPRLIAPPEVLPEPTPLPDPPPDLR"
     gene            complement(2324..2761)
                     /locus_tag="VNW28_09880"
     CDS             complement(2324..2761)
                     /locus_tag="VNW28_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10282.1"
                     /translation="MRACCRFSLTILCALALCGPALAETDQEVAARQVALELAGAFAN
                     DGFKLRDGHWVGTLAPHEGATVAVNLYAGNEYWFSAGATEQAKKLTIDVFDETGAPVT
                     TEKFNSGTKVAAGFSVTNSGEYFVRVRLEEGEPAGICFVYSYK"
     gene            complement(2763..3587)
                     /locus_tag="VNW28_09885"
     CDS             complement(2763..3587)
                     /locus_tag="VNW28_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10283.1"
                     /translation="MFVRNVSGAILWLLMSSAVFAVPSPPPVPSPAGLSQDQMAPAIK
                     TDSVTIPTPGELFAALAKPAKPDWAARYRGPIAMNYKTRAQIALNLGGLIADGFIAVE
                     AQDTQQVKNIGGDIIKMAKALGVSENILARGNSINQFAENDEWSTLQEELESTQNEVK
                     ASMQSHRDQDLIILVSLGGWIRGTQVVSGVVAKNYDAKLGQVLRQPELLRFIRSKIGQ
                     ISPELQNDPLVKSVNDQLSGIEQIVATPFGHGLSLAEVTKLSETVNKLMLEIQKKD"
     gene            3719..4420
                     /locus_tag="VNW28_09890"
     CDS             3719..4420
                     /locus_tag="VNW28_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HXA10284.1"
                     /translation="MRTRKLREKVVKVSGSHWVAYATASAATMFAGSQSADAAIHYSG
                     LLDVTMPGSGFHRVRLPLDQPGDSIAFEHRSSIFGFPDQWAGFRPVGRAGASFRGQGG
                     LSYSYVNKLQRGEFVSHGTSFTNAEQNSGFGELAGVISGYFDSRGIGFVGFAFDGGAG
                     KQYGWARVFMSGFSRGNGLKVLDYAYADPGEPIQVGQTSSDAQGTPALGSLGLLALGA
                     AGLVAWRKRRSDGAS"
BASE COUNT          877 a         1319 c         1417 g          881 t
ORIGIN      
        1 gtaaatgcaa accatcacca cgatcgtaaa gatattggac gtggcaaagg tggaaacaaa
       61 aagggtgagc gaggcgagca aggaagcttt caagtaaatg atggcgatgc ctgggagcag
      121 gttggcgttg aaggccgacg cccgcagacc gcggaccgcc tcctcgacct gggccggacg
      181 caggccggcg gtttcgcgca gtgtcgcgtt gatcaggatg tgttcccgga taaaaaggac
      241 ggcgagaaag accgcgctca tcgcgagcag gctgagcgcg agcaggagca ggacaccggc
      301 gagtttgccg agaagatatt cgtaacgccg gacgggtttg gcgagaatgg tgtagatggt
      361 gcgatcctcg acatcgcgcg gcaggagctg ggcggtggcc acgatcgcga ggagcgaggt
      421 gaaaatgctc attgcgccta acgagacatc cttcagcacc tggaattcct gctgaaaact
      481 gagcgaggcc atgaaaactg aattgccgat caggagaagc gcgaagagaa ggaggaaata
      541 gaaaatcttc agcctaacga gctcggtaaa agtgttgccg gtgatcgcgg cgacccgcga
      601 aagcgagaac ggacggtgcc cgggagcctt cattcctgat ggggcggcgc ggaggttgcc
      661 tcgagaaaga gccgctcaag cgtcgtctgc gactgacgct gcgcgaccag ccgggcgctt
      721 gatttcccga gcacggcggt gatttgcgcc aggatctcgg gcgtggcgtt ctccaggatc
      781 agctcggtct ggttttcaat cgcgagcaga tcttccaccc gcccttcgcg gacgaggact
      841 ccgtgggcga gaatgccgac gcgatcacaa acttcctgca cctgtgcgag gagatgcgac
      901 gaaagtaaca ccgtgatgcc ccgttgtttc aaatcgagaa tcagatcgcg aatttcactc
      961 gacccggcgg gatcgactcc ggccgtcggc tcatcgagca cgagcagttt gggctcctgg
     1021 atcagagcct gtgcgagtcc aatgcgctgg agcatgcctt tcgaatatcc gcccagccgc
     1081 cgggcgcgcg cctcctgcaa gccgacgagc gcgagcaact catcggtccg cgtccgcaac
     1141 ttttgaccgc gaatcccgca cagtttcccg taaaagcgga gcgtctcctc gccggtgaga
     1201 tatttgtaga aataaggatt ctccggcagg aaaccgaccg cttcgcggct ccgcacttca
     1261 cggctgtcgc gtccgaatat tttgctcccg cccgtggtcg gcgagatgag gccgagaatg
     1321 attttcagcg tcgtgctttt gccggatccg ttaggcccga gcaggccgta gacctgcccc
     1381 ggcgcgacct cgaggttcag tccgcgcacc gcgatcagtt ttttccggcg gaaagggctc
     1441 ggaaaaatct tcgtcagatt ctggacggcg acggcaggtt caatcatgac gaatctcgaa
     1501 accatgcgcg cgcgggggct cgggcggaag gaattcggtc tgtttgcgaa tgtgcccgcg
     1561 cagctcgctt tgcccgatcg cttcgaggaa ggcgcgcact tcggtttcct cgccgctgat
     1621 ttgcaattcg acgcgaccat ccggcaggtt gcgcacccaa ccgaccagat cgaagccctt
     1681 ggccaggtgc ttgattgacc agcgaaagcc gacgccctgg acgttgcctt caaagaaaac
     1741 ctgcagcgaa atcataaggg aatcgtggcg caaggtagga gcttgcccgg agccggggga
     1801 gacgcaagcc gggtgttttt gcgagcaagg gcaggctcgt ccgtccgacg aacaacaaag
     1861 gcgcggcctt aacggagatc cggcggagga tctggcaggg gagtgggctc gggtagcacc
     1921 tcgggcggcg cgatcaggcg cgggtcatcc gagttgagtt gaaactcttc gccttgcccg
     1981 gcgaaggtcg gtgtcggggt gggtggcggc gcgagctttt ccgtcaccag cgcggccggg
     2041 gcgggccgga tatcttcgct tgcgacgtag ccgctttcgc catcctgcaa ttgcactttg
     2101 acgtacccga acgacggccg gatcactttc atgatcgaat ccttcggcag ttgcgaatcg
     2161 ggcccactgc cctgctgcgg gccatagtgg taaaacggag tctggttggc cgcgactgag
     2221 taccaggtgt tggtcgtccc ggagagcgcg ctcagcttga tcgacgaaac acagcccacc
     2281 gccaggagag cgacggctcc cagagggatg aggcagcgga gtttcatttg taactgtaaa
     2341 cgaaacaaat tcccgccggc tcgccctctt cgaggcgcac cctgacaaaa tactcgccac
     2401 tattggtcac ggagaacccc gcagcgactt tcgtgccgga attgaatttt tccgtcgtaa
     2461 ccggcgcgcc ggtctcgtcg aaaacgtcga tcgtcagttt ttttgcctgc tccgtcgcgc
     2521 cggcggaaaa ccagtactca ttcccggcgt agagattgac cgcgaccgtt gccccctcgt
     2581 gaggcgcgag ggtgccgacc caatgtccgt cgcgcagttt gaagccgtca ttggcgaacg
     2641 cgcccgccag ctcgagtgcc acctgccgcg ccgcgacttc ctggtcggtc tccgccaggg
     2701 ccggcccgca gagcgcgagc gcgcaaagaa tcgttaggga aaagcggcag caggcgcgca
     2761 tgtcagtcct tcttttgaat ctccagcatc agcttgttaa cggtctcact cagcttcgtc
     2821 acttccgcga gggagagtcc gtggccgaag ggcgtggcga caatctgctc gatcccgctc
     2881 aactggtcgt tgacgctttt gaccagcggg tcgttctgca actccggcga gatttgtccg
     2941 atcttggaac ggatgaaacg cagcagctcc ggctggcgca ggacctgccc gagcttcgca
     3001 tcataattct tcgccaccac cccgctgacc acctgggtgc cgcgaatcca gccgccgagg
     3061 ctaacgagga tgatcaggtc ctgatcacgg tgcgattgca tggaagcttt gacttcgttc
     3121 tgagtcgatt ccagttcctc ctggagcgtg ctccactcgt cgttctcggc gaactgattg
     3181 atgctgttgc cacgcgccag gatattttcg ctcaccccca gggccttcgc catcttgatg
     3241 atgtcgccgc cgatgttttt tacctgctgc gtatcctgcg cctcgaccgc gatgaagccg
     3301 tcggcaatca agccgccgag gttgagtgcg atctgcgcgc gggtcttgta attcatcgcg
     3361 atcggtccgc gatagcgcgc ggcccagtcc ggcttcgcag gtttggcgag ggcggcgaag
     3421 agttcgcccg gggtcgggat ggtgaccgag tcggtcttga tcgccggcgc catctggtct
     3481 tgtgaaagcc cggcgggcga gggcacaggc gggggcgacg gaaccgcgaa gacggcgctg
     3541 ctcatcagca accaaagaat cgcaccgctc acattccgca cgaacatgac ggcagattag
     3601 ccacgccgga gggcattgcc aacgcgaaat attttcgagg gccggagtgg aactccaggc
     3661 gagaacgctc cgtccgtatt tcttgacctc aaggagggat cctgagcatt ctccgcgaat
     3721 gaggacgcgc aaactccgcg agaaggtcgt taaggtttcg ggttctcact gggtcgccta
     3781 cgccactgcg agcgcagcca ccatgttcgc tggcagtcaa tccgcggacg cagcgatcca
     3841 ttattccggc ctgctggatg tgacaatgcc ggggtctgga ttccaccgcg tgagattgcc
     3901 actggatcag cccggcgatt ccatcgcctt tgaacaccgc tcatccatct ttgggttccc
     3961 cgaccagtgg gccggcttca gacccgttgg ccgcgcgggc gcttcttttc gcggacaggg
     4021 cggactctct tatagctacg tgaataaatt gcagcggggc gaatttgtat cgcatggtac
     4081 ctcctttacc aacgccgagc agaactctgg cttcggagaa ctcgccggtg tcatctcagg
     4141 atacttcgat agtcgcggta tcggatttgt cggattcgcc tttgacggcg gcgcggggaa
     4201 gcaatacggc tgggcgcgcg tcttcatgag tggtttttca cgaggcaacg gcctcaaggt
     4261 cttagactac gcctacgccg accccggcga accgatccag gttggccaaa catcaagcga
     4321 tgcgcagggg acgcccgcgt tgggttcgct tggccttctt gcgctggggg ccgctggcct
     4381 cgtcgcctgg cgcaagcggc gatcggatgg cgcgtcgtag acgtaaggtt tgtccctatg
     4441 caggtgcgac gaagcgcgca acagacgcgc gcgtggcgga attttcttga gctt
//
LOCUS       DATMNS010000285         9120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2094382, whole genome shotgun sequence.
ACCESSION   DATMNS010000285 DATMNS010000000
VERSION     DATMNS010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9120
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094382"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..798)
                     /locus_tag="VNW28_09895"
     CDS             complement(<1..798)
                     /locus_tag="VNW28_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXA10285.1"
                     /translation="MKALCFEKFGGPEVLEYREVPDPIPAPGAALVRLSAVGLNFADI
                     YRRRGNYHLAGAPPYIPGYEGAGVVEMVPAENVAPNDLPMIKVGQRVAFADSPHANAE
                     LAVVSLEKVIPLPEDISFATAAALLLQGLTAHYLTRDSHLVAPNEQTVVHAAAGGVGL
                     LLVQIIKLLGGKVIALTSSEAKRAAALDAGADAVVLYGDDWPDSVLKLTREGKGVDVI
                     YDSVGATLDASLRVTRKGGQVVFYGMAGGDPAFVDPRRLMDESKTLTG"
     gene            964..2763
                     /gene="pepF"
                     /locus_tag="VNW28_09900"
     CDS             964..2763
                     /gene="pepF"
                     /locus_tag="VNW28_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HXA10286.1"
                     /translation="MDETKIPTRDDIPETDKWDLSHLFTSADKWSEDFAWIQATYPRL
                     TTWKGRVGESAATLAELLEFDKTLDLKIERVYHYASLQLAEDSSNPDYLARMGQLQNL
                     LTKIGETASFVAPEIQAIADEKFAQFLAEPALQEWQISLHKIRRMKPHVLSEKEERLL
                     ALGSAALSGYDDAFSQLTDVDMKFGVLRDDKGEERPLSQSSFSSFLVKRDHDLRKRAF
                     HQFYAEFQDHQFTLAAALSYSVKADVFRARARNYSSALEASLFKDDIPSAVYDGLIAA
                     VRQNLTPLFRYYDLRRRVLGLRELHHYDTYVPLVSEIETDVSFDEAITDVLASLAPLG
                     KEYVETLGTGLRGRWCDRYESKGKRSGAFSSGSYGAPPYILMNYKRDVFSDIFTLAHE
                     AGHSMHTWFAQRTQLYQDADYPIFLAEVASTFNEELLTHHLLARTTDPKMRAYIINRQ
                     IDDIRGTLFRQTMFAEFEKIIHALEESGAALTLDVFKSEYRKLLEAYFGPGVVLDPEL
                     ELEGLRIPHFYNAFYVYKYATGLSAATALAERVTTGVPGAVEAYLGFLRSGGSKFPLE
                     TLQAAGVDMRTSAPVESTLRLFDRRVTELEGLL"
     gene            2948..3364
                     /locus_tag="VNW28_09905"
     CDS             2948..3364
                     /locus_tag="VNW28_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10287.1"
                     /translation="MDRESMAELNAIPKAMQDHPEVLQLRLHHLMMRKSWQRALALSR
                     KLCRLVPRSGAGFLHAGFCLNQLGRTAEARAMLLKGPPALRREPIYYYNLGCYEALLG
                     HPQDARRHLETSFRMDASFREIAKEDPDLKAVQPLL"
     gene            3461..4564
                     /locus_tag="VNW28_09910"
     CDS             3461..4564
                     /locus_tag="VNW28_09910"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HXA10288.1"
                     /translation="MVTDPGYNCLVEREVELRIEDIAFGGKGVGRAEGKAIFVPFTID
                     GERVAARVVREKKSFAEADLVSVLEPSPQRATPQCPYFGRCGGCSYQHMSYEHQLHWK
                     ARQVRQALQRIGKFREPPVRPIVPSPQDYEYRNRITVHAEDGTVGFFRRDGHRLIDIE
                     RCPIAAPAVNDELTALRARRPGDGHYTLRAESGPRVFSQTNDAVATHLADAIAALIPP
                     NESLLIDAYCGAGFFAKRLLAKFSRVVGIDWDIHAIAAAESNATAAETYLAGEIAAHL
                     GEQLAGANLKSTSVIVDPPATGLSADVREILRRSPAATLLYVSCNPPTLARDLADLGP
                     AFTLQSVTPFDMFPQTAEIEVLAHLTGCESAAR"
     gene            4654..5973
                     /locus_tag="VNW28_09915"
     CDS             4654..5973
                     /locus_tag="VNW28_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXA10289.1"
                     /translation="MHILIVDDEKNIRRALTMALESMEHAVTCAANSATALAELRTAS
                     FDVVLLDLKLSQENGLDVLEEILRISPQTAVVMVTAYASIETAVEAMRRGAFDYLPKP
                     CTPDQVRQVLGRIERTRKLERRVVELESRLGADGPESDLTSQSPAMQKVLEIAFKVAE
                     SEAIVLLLGESGTGKSVLARAMHQRSPRRGGAFVTVSCPSLSRELLQSDLFGHVKGAF
                     TGAYADTLGKVAAADGGTLFLDEIGELPLEIQAKLLRLLQEREYERVGESNPRRANVR
                     VISATNRDLAQAVAEGKFREDLYYRLNVISLRLPPLRDRMEDLEKIAVSHLTFLAGHA
                     GKNVRGFSREALAAMRQYAWPGNLRELHNVIERAVILCSGEVIEPDDLSDTIQTSSEL
                     RLGGKLTVHEVETEHIRRVIASTRTLDEAATILGIDPATLYRKRKKL"
     gene            6304..8112
                     /locus_tag="VNW28_09920"
     CDS             6304..8112
                     /locus_tag="VNW28_09920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXA10290.1"
                     /translation="MKRRIFFGLAPLFILLIAMGLYAVTLFTNLGNRVDVILRENFRS
                     VLAGQQMKETAERMDSALSFSLVGEEERGRKLYAQNLPGFRENLRIELDNITLPGEAE
                     LAAKVKQLHEKYAARAEAFWATPDIEARRKMYFGEMLPVFTEIKDTAQEIIRINQDNM
                     VQADREARRLAAKSTRYMIFAIAAGIGGAIFFALRLQRSILRPIRQLTTVSKELGEGK
                     LDQVVPVASQDELGQLADTFNKMATKLRAYRQVMGDQILQARQMTEITFSAFPDPIIA
                     LTSDGRIDFTNPAATKFLYHCGHADSLPASVQAEVDRVFKGAPDFLPTSFERVIVFRV
                     EGHEVFMLPRVIGMRDESGNIFGAAVILQDVTRLRLLDEVKTNLVSTVSHELKTPLTS
                     VRMGLHLLLEERIGPLNNKQTELLLAAREDSERLLRMINDLLDLAKLESGKAVLPSEV
                     IEPGELVQSAGEDLRGLVESRGSRLVTKTEPDLPRVFVDAQQISHVFSNFVSNAIKHT
                     RAGEEIVLGAKKHNDTVRFSVIDHGSGIAAKYRARIFDRFFRVPGAEVTGAGLGLAIA
                     REIVLAQGGTIGVNSTPGQGSEFYFDLPPARNGASS"
     gene            8109..8801
                     /locus_tag="VNW28_09925"
     CDS             8109..8801
                     /locus_tag="VNW28_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA10291.1"
                     /translation="MKAKILIVDDEPNVRLSYRVALETEGYVVKEAHCGARALDELAA
                     GGFDLAILDMRMPEMDGLDLLAEMRNRGFTTPTVIITAFGDVPHAVRAMKLGAIDFLK
                     KPITPQALRNIVTDVVSRHQTKATPASPPRDDFHDHIVTAQRLLNFQDFAQAKKHLVR
                     ALELNDKSPEAFNLTGVLFEMQEDYERAKKYYGQAIKLNKHYEPAQQNMRRVFELFHF
                     GTSKEPIALGDD"
     gene            8919..>9120
                     /locus_tag="VNW28_09930"
     CDS             8919..>9120
                     /locus_tag="VNW28_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10292.1"
                     /translation="MLRANASSLAITLALLASTVALSSARAGEQTEEAKATASPTPES
                     RFKIYGWVDGGITFNPAGPRDNQ"
BASE COUNT         1872 a         2755 c         2754 g         1739 t
ORIGIN      
        1 accggtcaga gttttcgact cgtccatcag ccggcgcgga tcgacgaaag ccggatcgcc
       61 gcccgccatc ccatagaaga caacctgccc gcctttgcgc gtcaccctca gactcgcgtc
      121 gagcgtcgcg ccgacggagt catagatgac atccacgcct ttgccctctc tggttaactt
      181 caagaccgag tccggccagt cgtcgccata gaggacgacc gcatctgcgc cggcgtccag
      241 cgcggcggcg cgcttcgcct cgctcgaggt gagggcgatg acctttccgc cgagcaactt
      301 gatgatctgc acgagaagca gaccgacacc cccggccgcg gcatggacga cggtctgctc
      361 gttaggcgcg accaggtggc tgtcccgggt caggtagtgc gcggtgagac cttgtagcaa
      421 aagcgccgct gcggttgcaa aagaaatgtc ttccggcaac ggaatcacct tttccagaga
      481 aaccaccgcc agctccgcgt tggcgtgcgg gctgtcggcg aaggcgacgc gctgacccac
      541 tttgatcatc gggagatcgt taggtgcgac attctccgca ggaaccatct cgaccacgcc
      601 cgcgccttca tagccgggaa tgtagggcgg cgcgccggcc agatggtaat tgccgcgccg
      661 gcggtagatg tcggcgaagt tgagtccgac cgcgctcagc ctcacgagcg cggcgcccgg
      721 cgcgggaatg ggatcgggca cctcgcggta ctccaggacc tcaggtccgc cgaacttttc
      781 gaaacagagt gctttcatgc tttcgatttt ggggcctgca tccgtgatac gtatagcacg
      841 acgaaagaat ttgtggggga gcgcacgcgg cggacgagcg gaagctcgtc cctccggaga
      901 gactcgtcct ctctcggctc ttctcgggat gacagaatgg ctatttgctg cgagaaaaac
      961 aggatggacg aaacgaagat cccgacgcgc gacgacattc ccgagactga caagtgggat
     1021 ctgtcccacc ttttcacgag cgccgataaa tggagcgagg actttgcctg gatccaggcg
     1081 acttatccgc gcctcacgac gtggaaaggg cgggtcggcg aatcggccgc gacactggcg
     1141 gagttgctcg agttcgacaa gacactcgat ctgaaaatcg aacgtgttta tcactacgct
     1201 tcgctccaac tggccgagga cagctcgaat cccgattatc tcgcgcgcat ggggcagttg
     1261 cagaatctgc tcacgaaaat cggcgagacc gcctctttcg tcgcgccgga aatccaggcg
     1321 atcgcggatg aaaagttcgc gcagtttctc gccgagccgg cgctgcagga atggcaaatc
     1381 tccctgcaca agatccggcg gatgaaaccg cacgtccttt ccgaaaaaga agaacggctg
     1441 ctcgcgctcg gcagcgcggc gctcagcggc tacgacgatg cgttttccca gctgacggat
     1501 gtcgacatga aattcggcgt gctgcgcgat gacaaaggcg aggaacgccc gctctcacaa
     1561 agttccttca gctcgtttct cgtgaaacgc gatcacgacc tgcgaaagcg cgcctttcac
     1621 cagttctacg cggagtttca ggatcatcag ttcaccctcg cggcggcgct ttcctattcc
     1681 gtgaaagcgg atgttttccg ggcgcgggcg cggaattatt cgtcggcgct ggaggcttcg
     1741 ctcttcaaag acgatatccc gagcgcggtt tacgacgggt tgatcgcggc cgtcaggcaa
     1801 aatctgacgc cgcttttccg ctactacgat ctgcgccggc gcgtccttgg attgcgcgaa
     1861 ctgcaccact acgacaccta tgtgccgcta gtcagcgaga tcgaaacgga cgtttctttt
     1921 gacgaagcga tcaccgatgt gctcgcttcg ctggcgccgt taggcaagga atacgtcgag
     1981 acgttaggca cggggctgcg cgggcgctgg tgcgaccgct acgaatcgaa aggcaagcgg
     2041 agcggagcgt tttcgagcgg cagctacggc gcgccgcctt acatcctgat gaattacaag
     2101 cgggacgtgt tctcggatat tttcacgctg gcgcatgagg cgggccattc gatgcatacc
     2161 tggttcgcgc aacggacaca gctctatcag gacgccgatt atccaatctt tctcgcggag
     2221 gtcgcttcga cctttaacga agaactgctg acccatcatc tcctcgcgcg cacgaccgac
     2281 ccgaagatgc gcgcctacat catcaatcgc cagattgacg acattcgcgg cacgcttttc
     2341 cggcagacga tgtttgccga gtttgagaaa atcatccacg cgctcgagga aagcggcgcg
     2401 gcgctgaccc tcgatgtttt caagagcgag tatcgcaagt tgctcgaggc ctacttcggc
     2461 ccgggcgtgg tgctcgatcc cgagctcgag ctcgaaggcc tgcgcatccc gcatttttac
     2521 aacgcgtttt acgtttacaa atatgccacc gggttgtcgg ccgcgaccgc actggcggaa
     2581 cgcgtgacga ccggagtgcc gggagcagtc gaggcctatc tcggttttct gcggtcgggc
     2641 ggctcgaagt ttccgctcga gactttgcag gcggcgggtg tcgacatgcg cacctccgca
     2701 ccggtcgaga gcactctacg gctcttcgat cgtcgggtga ccgagttgga aggcctcctg
     2761 tagacgccgc tctgcgggcg gcgcggggaa aagctgtctt ttccagcggg ccgaccctcg
     2821 cgcttcgcgc agcgaagttg ctacaagccg cctcaaaatc cagccgaggc ccgcgatttg
     2881 ctatttccag aagccgatga ggctcgaatg gcagttgcgc agtgcctgcg gctacgcaga
     2941 acttggtatg gaccgcgaat ccatggccga gctgaacgcc atccccaagg ccatgcagga
     3001 tcatcccgaa gtgctccaac tgcggctcca ccatttgatg atgcgcaaat cgtggcagcg
     3061 cgcgctcgcc ctgagccgga agctctgccg tctcgtgccc cgaagcggcg ccggattcct
     3121 ccacgccgga ttttgtttga accagctcgg tcgaacggcc gaagcgcgcg ccatgttgct
     3181 gaaggggccg cccgccctgc gtcgggaacc gatatattac tataatctcg gctgctacga
     3241 ggcgcttctc ggccatccgc aggatgcgcg gcggcacctg gaaaccagtt ttcggatgga
     3301 cgcgagcttc cgcgagatcg cgaaagagga tcccgacctg aaggcggttc agccgttgtt
     3361 gtagccggga tcggtgatcc ccgactctcc tcccctttca atgcttgaac gaccgccaac
     3421 ggagcagcgc gttcgcccgg cgcggccatt ggctctcgcc gtggtcaccg accccggcta
     3481 caactgtctg gtggagcgag aagtggaatt gcggatcgaa gacatcgcct tcggcggaaa
     3541 gggcgtcggg cgcgcggaag gcaaagccat ctttgtccct ttcacgatcg acggggagcg
     3601 ggtcgcggcg cgcgtcgtgc gggagaaaaa atcgttcgcg gaagcggatc tggtttcggt
     3661 cctggagccc tcgccgcagc gcgccactcc acagtgccct tattttgggc gatgcggtgg
     3721 ctgcagttat cagcacatga gttacgaaca tcagttgcac tggaaagcgc gccaggtccg
     3781 gcaggcgctg cagcggattg ggaaatttcg cgagccgccg gtgcgcccga tcgtcccctc
     3841 gccgcaggat tatgaatatc gcaaccggat cacggtgcac gccgaggacg ggaccgtcgg
     3901 atttttccgg cgcgacggcc atcggttaat cgacatcgaa cgctgcccga tcgccgcgcc
     3961 ggcggtgaat gacgaactca ccgcgctccg cgcccggcgc ccgggcgacg gccattacac
     4021 tttgcgtgcg gagagcggcc cgcgcgtttt ctcgcagaca aacgacgcgg tcgcgaccca
     4081 tctcgcggac gcgatcgcgg ccttgattcc gcctaacgaa tcgctcctga tcgacgccta
     4141 ctgcggcgcc ggtttcttcg ccaaacgcct gctcgcgaaa ttctcgcgcg tcgtcgggat
     4201 tgactgggac atccacgcga tcgccgccgc ggagtcgaac gcgaccgcgg cggaaaccta
     4261 ccttgccggg gagatcgccg cgcatctcgg agagcaactc gcgggcgcca atcttaaaag
     4321 cacgagcgtg atcgtcgatc cgcccgcgac cggcctgagt gccgatgtgc gcgagatcct
     4381 ccgtcgctct ccggctgcga ctcttcttta cgtctcctgc aatccgccga ccctcgcccg
     4441 cgacctggcc gatctgggtc cggctttcac cctccaaagc gtgacgccgt tcgacatgtt
     4501 cccgcagacc gccgagatcg aagttctcgc ccatctcacg ggctgcgaga gcgcagctcg
     4561 ttaacgaatt cttacgtcgg cgggcgttcc tttgattggc gcggatgcga agtcgagttc
     4621 ccaagcggga gcgacaaggg taaggtccga cggatgcaca tcttgatcgt tgacgacgaa
     4681 aagaacattc gccgcgcctt gaccatggcg ctggaatcga tggagcatgc ggtgacctgt
     4741 gctgcgaata gcgccacggc cctggcggaa ttgcgcaccg cgtcgttcga tgtcgttctc
     4801 ctggatctaa agttgagcca ggaaaacgga ctcgatgtgc tggaagaaat cctgcgtatc
     4861 tcgccgcaga ccgcagtcgt gatggtgacg gcttatgcgt caatcgagac agcggtggag
     4921 gcgatgcgcc gcggagcgtt tgattacctg ccgaaaccct gcacgccaga tcaggtgcgg
     4981 caggtgctcg ggaggatcga gagaaccagg aagctggaac gccgggtcgt cgagctggag
     5041 tcgcgtctgg gagcggacgg accggaaagc gatctcacgt cgcaatcgcc cgccatgcag
     5101 aaggtgctcg agatcgcatt caaagtggca gagagcgaag cgatcgtact ccttctcggc
     5161 gagagcggca cgggcaagag cgtgcttgcg cgagcgatgc atcaacgaag tccgcgccgc
     5221 ggcggagcct ttgtgacggt gagttgtcca agcctttcac gagaactgtt gcaaagcgat
     5281 ttgttcggtc acgtgaaggg cgcgttcacc ggcgcttacg cggacacact gggcaaggtc
     5341 gcggcggccg atggcgggac gttgtttctt gacgagatcg gtgagttgcc actggagatt
     5401 caggcaaaac tgctgcggct cttgcaggag cgagaatatg agcgcgtcgg cgaatcgaat
     5461 ccgcgccgcg ccaacgtgcg cgtgatctcg gcgacgaacc gcgatctcgc gcaagcggtc
     5521 gcagagggga agtttcgcga agatctctac taccggctga atgtcatctc gctccgtctg
     5581 ccgccgctgc gcgatcggat ggaggacctc gagaagattg cggtcagcca tctgaccttt
     5641 ctcgcaggac atgcgggcaa gaatgtgcgc ggcttttccc gtgaggcact ggccgcgatg
     5701 cggcaatacg cgtggccggg caatctccgc gagcttcaca acgtgatcga gcgggcggtg
     5761 atcttgtgca gcggcgaggt gattgagccg gacgatctgt ccgacacaat tcaaaccagc
     5821 tcggagttgc ggctcggcgg aaaactcacc gtgcacgaag tcgagacaga gcacattcgg
     5881 cgggtgatag cgagcacgcg caccctggat gaagcggcaa cgattcttgg cattgatccg
     5941 gccacgctct accgcaagcg caagaagctc taatgcgaga gtggccggcg ttttcgcgtc
     6001 gcgcaaaatg caatccgcaa ggcgtaccaa tcgtgcatga tgcacgactc gctttgaatt
     6061 aatgaaataa cgtgagatcg caaatggctc tcttgcacgg tttaacgtgc tcatagaaaa
     6121 gcctggcacg ccgttcgcta acgaggcggg catgaagatg attcgccagt cgccgcaacg
     6181 ggtctccggc gcgcaagcga gtcggacgcg cgccagttat ggaacaccaa gtggccgaga
     6241 agcacggtcg ccatctaatt tggccgctcg tcacgaaaag gtgcgtcgag cacgctcggt
     6301 ggcatgaaac gacgcatctt tttcggtctc gcgccgctct tcattctgct catcgcgatg
     6361 ggtctctatg ccgtcacgct tttcacaaat ctcggcaacc gcgtcgatgt gatcctgcgt
     6421 gaaaacttcc gcagcgttct ggccggccag caaatgaaag agactgctga acggatggat
     6481 tcggccctct ccttctcgct cgtgggcgag gaagagcgag ggcgcaaatt gtacgcgcaa
     6541 aacctgcccg gatttcggga aaacctgcgc atcgaactcg acaacatcac tctgccgggt
     6601 gaagccgaac tcgccgcgaa ggtgaaacag ttgcacgaga aatacgccgc tcgggcggag
     6661 gccttctggg caacgcccga catcgaggcg cgacggaaga tgtatttcgg cgagatgctg
     6721 ccggtcttca cggaaatcaa ggacacggcg caggaaatca tccgcatcaa tcaggacaac
     6781 atggtgcaag cggaccgtga ggcccgccgg ctcgcggcga aatcgacccg ctacatgatc
     6841 ttcgcaatcg ccgccggcat tggcggcgcg atcttttttg ccctgcggtt gcagcgctcg
     6901 attttgcgtc cgatccgtca gctcacgacc gtctcaaagg aacttggcga ggggaaactt
     6961 gaccaggtcg taccggtcgc gtcccaggac gaactcggcc aacttgccga caccttcaat
     7021 aagatggcga ccaagctgcg cgcttatcgc caggtgatgg gcgaccagat tctacaggcg
     7081 cgacagatga ccgagatcac tttttcggcg ttccccgatc caatcatcgc gctcacttcc
     7141 gatgggcgga tcgattttac gaatccggca gcgaccaaat tcctctacca ttgcgggcac
     7201 gcggattcgc tccccgcgag cgtgcaggcg gaggtcgatc gcgtctttaa aggcgcgccc
     7261 gattttctgc caacgagctt tgagagagtg atcgtttttc gagtggaagg gcacgaggtc
     7321 ttcatgctcc cgcgcgtgat cggaatgcgg gacgaatcgg gcaacatctt cggcgccgcc
     7381 gtcattctcc aggacgtgac ccgtctgcgc ctgctcgatg aggtaaagac gaatctcgtc
     7441 tcgactgtca gtcacgagtt gaagacgccg ctgaccagtg tgcgcatggg cctgcacctt
     7501 cttctcgaag aacgcatcgg ccccctgaat aacaaacaaa cggagttgtt gctcgccgcc
     7561 cgggaggatt ctgagcggtt gctgcggatg atcaatgacc tgctcgatct cgcgaaactg
     7621 gagtcaggca aagccgtgct gccgagcgaa gtaatcgagc caggggagtt agtccaaagt
     7681 gcgggagaag acttgcgcgg gctcgtcgag tcacgcggct cgcggctcgt gacgaaaacc
     7741 gagccggatt tgccgagagt cttcgtcgat gcgcaacaga tttcgcacgt tttttcgaat
     7801 tttgtctcca atgcgatcaa gcacacgaga gccggcgaag agatcgtgct cggcgcaaaa
     7861 aagcataacg acactgtccg cttttccgtg atcgatcacg gctccgggat tgccgcgaaa
     7921 tatcgggcgc gcatcttcga ccgcttcttc cgtgtgccgg gtgcggaagt gacaggcgcg
     7981 ggtctcggcc tggcaattgc cagggaaata gtcctggcgc agggcgggac gatcggcgtc
     8041 aacagcaccc ccgggcaagg gagcgaattc tatttcgatc tgccacccgc ccgcaacggc
     8101 gcaagttcat gaaagcaaaa atactaatcg tagatgacga gccaaatgtc cggctcagct
     8161 atcgcgtagc cttggaaacc gaaggttacg tcgtcaagga agcgcattgc ggcgcgcggg
     8221 cactcgatga gctggccgcc ggcgggttcg atctcgcgat cctcgacatg cgcatgccgg
     8281 agatggatgg actcgatctt ctcgcggaaa tgcgcaaccg cggtttcacc acaccaactg
     8341 tcatcatcac ggcctttggc gatgtgccac acgccgtccg cgcgatgaag ctcggtgcga
     8401 tcgattttct caagaaaccg atcacgccgc aggcgctgcg caacatcgtt acggatgtgg
     8461 tttcacgtca ccaaaccaag gccactccgg caagcccgcc gcgcgatgac ttccacgatc
     8521 acatcgtgac cgcgcagcgg ctcctcaact ttcaagattt cgcgcaagcc aagaagcacc
     8581 tcgtccgcgc gctcgaacta aacgacaaat cgcccgaagc cttcaacctc acgggggtgc
     8641 tcttcgagat gcaggaggat tacgagcgcg cgaagaaata ctacggccag gcgatcaagc
     8701 tgaacaaaca ttacgagccg gcgcagcaga acatgcggcg cgttttcgag ttgttccatt
     8761 tcggcaccag caaggaacca atcgccttgg gggatgacta accaacatga cgagacgaat
     8821 ccttgtcatc gatgagacgc cgaaatatcc ggcctggtta ttgataacag ccaattctta
     8881 gatacctcct gccaaaacat tctttaaaac accatcccat gcttagagcc aacgcttcat
     8941 cactcgcaat caccctcgcc ttactcgcaa gcacagtcgc gctctcctcg gcgcgcgccg
     9001 gcgaacaaac cgaggaggca aaggcgactg cttcacctac gcctgagtcg cgtttcaaga
     9061 tctatggctg ggttgacggt ggcatcacct tcaatcccgc cggcccgcga gataatcaga
//
LOCUS       DATMNS010000286         6528 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3526716, whole genome shotgun sequence.
ACCESSION   DATMNS010000286 DATMNS010000000
VERSION     DATMNS010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6528
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3526716"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..834)
                     /locus_tag="VNW28_09935"
     CDS             complement(217..834)
                     /locus_tag="VNW28_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HXA10293.1"
                     /translation="MITQRQAQLSKDADAKRAEGNYVEAINLYEAALDGRPDSADVHY
                     KLALIYDDKLNDPLNALHHFKRFLALEPGGKRAEEVKGFMKRDELTLLTNLTGDSMVP
                     RSEVVRLRNENLNLRQQIEGRWAEKKAASATEKTASGTKKQPVEKEKKGGPARHYTVQ
                     RGDTLASIARKFYKSSARWHRILEANSDTLSKPGDLKPGQTLLIP"
     gene            complement(983..2170)
                     /gene="moeB"
                     /locus_tag="VNW28_09940"
     CDS             complement(983..2170)
                     /gene="moeB"
                     /locus_tag="VNW28_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HXA10294.1"
                     /translation="MGAGENITTSSPNEKAGASLGEEERRRYSRHIMLPEIGVAGQER
                     LKAARVLCLGTGGLGSPAALYLAAAGVGTIGLVDDDRVALSNLHRQLLHGTKDVGRPK
                     TESARDRLVETNPHVAVQLHACRFTSGNAEGILRDYDLIVDGTDNFATRYLSNDVAVF
                     ARKPNVYGSIFRFDGQATVFAPHLGGPCYRCLFPEPPPPGAVPSCAEAGVLGVLPGMI
                     GTLQATEALKLILGLGETLVGRLVHFDALKMKFREFNLRRDPDCPVCGRQPTITAPID
                     YETFCHGGPDLTAAVPQIQVRALEQRMASGEPYILLDVREPFEFELARIAGANLIPLG
                     QLPARFGELDREKEIFVFCHSGVRSERAAEFLRAAGFPKIANVAGGIDAWSEEIDPDV
                     PRY"
     gene            complement(2149..2436)
                     /locus_tag="VNW28_09945"
     CDS             complement(2149..2436)
                     /locus_tag="VNW28_09945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10295.1"
                     /translation="MQLLNTRTARFTDVVEACGTPQVYTLWLAPNKDRRLQAQIRQSR
                     IMTIQRSAGGAEFGCVGFVARQNALYLSFSKSLKRFADQRIVGMKWELVKT"
     gene            complement(2672..2766)
                     /gene="ffs"
                     /locus_tag="VNW28_09950"
     ncRNA           complement(2672..2766)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VNW28_09950"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(2915..3964)
                     /locus_tag="VNW28_09955"
     CDS             complement(2915..3964)
                     /locus_tag="VNW28_09955"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HXA10296.1"
                     /translation="MIASDAQLAEILPRFRSFARVAVDTEADSLHCYFEKLCLIQLTF
                     DGQDYLVDPLASIDLQPLCTALAEKEIVLQGMDFDLRLLRRSFNFDVREVFDTVIAAR
                     MLGLTEFSLAALVQQYFGVTLAKGSQKANWARRPLPRAMEEYAKNDTHFLLPLAARME
                     EQMEALGRTGWFRQSCQRALEQTRVTRERDPDEAWRIAGSGTLPGQTGAVLRAIWQWR
                     DSEAKDADRPAFHILQNSAMIEAAKQFVAGETPEFRHLSERRRRGFLAAARAALELPE
                     SAWPQRPPRVNRARTRDFDRKVEELKRRRDQHAKELALEPSFIAPRSALEALAADPSR
                     AETVLVAWQRELLGL"
     gene            complement(3961..5304)
                     /gene="rho"
                     /locus_tag="VNW28_09960"
     CDS             complement(3961..5304)
                     /gene="rho"
                     /locus_tag="VNW28_09960"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXA10297.1"
                     /translation="MDEEKFTAPENAGPATLLPEPEEKDRPPFEPLELSALRDLAPAA
                     LQDLFVRFDLQPHPSRTRHQLILDLVRHAVSRGQEVRTSGFLEQPNDGPAMLRSPELN
                     FLPLPEDAGVAHHLIRQFNLRPGQLLAGAIRLPREREKGMMLDRILSIEGQPAEAWQA
                     PTPFDHLTPLFPQGRIMLENDSADSACVRAVDLLTPLGRGQRGLIVAPPRVGKTILLK
                     QIAKAIRMNHPDIVLILLLVDERPEEVTDLQREVDCQIYSSTFDEPPTRHVQVAELVS
                     ERAKRLVEQKKDVVLMLDSITRLARGYNALIRGKGRTMSGGVDAKALLKPKRFFGAAR
                     NVEEGGSLTILATALIETGSRMDEVIFEEFKGTGNMELHLDRSLVEKRLYPAIHILQS
                     GTRREDLLYHPEEWERVKMLRKTMAALPPLEAMELLINNLHATKTNAELLLAGLR"
     gene            complement(5304..5912)
                     /gene="coaE"
                     /locus_tag="VNW28_09965"
     CDS             complement(5304..5912)
                     /gene="coaE"
                     /locus_tag="VNW28_09965"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXA10298.1"
                     /translation="MPESCSVIGITGGISTGKSTFCNRLSQLLRAKPFDADRAARQLV
                     EQDPEVRRLLRERFGPEIFSAGGDLNRAALRAIVFADQGKKRALEQILHPRIRRQWAT
                     EAEESRQSGEIFLADIPLLYETKGEVLCDRVVVVACASEIQQKRLISRTGLTPSDAHA
                     MICSQMPLPQKISRADHVVWNNGPVPNLEAQAGILARFLAAK"
     gene            complement(6173..>6528)
                     /locus_tag="VNW28_09970"
     CDS             complement(6173..>6528)
                     /locus_tag="VNW28_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10299.1"
                     /translation="ILISIGPTETMHFQTWADKAGNAPPLTDPTNGLVFPDLNSPPFG
                     GQNFQTNLIMPEPCNFISPNLPVCSIIRPTETDGIAMGVVNFLTAMGLFIGQSSEFFA
                     TLNALAAAADAAERS"
BASE COUNT         1261 a         2035 c         2004 g         1228 t
ORIGIN      
        1 gctttcataa gaggcaatgc ccgccaagat aaaacgaaac cataacttcg tcatcccggg
       61 gagggttgcc gccgcgcaga ccagagcgaa aatccgacca gagcgaaaat ccgaaatccg
      121 aatctcgaaa tccgaaacaa attcgaaatc taaattcagg aaaaactggg aaatctcgaa
      181 accgggatgc ccccaggttc tgtctgtttc ctaccgctac gggatgagca gcgtctgccc
      241 cggtttcaaa tcgcccggct tggaaagcgt gtcggaattg gcctcgagga tcctgtgcca
      301 gcgcgccgaa ctcttataga acttgcgtgc aatcgaagcc agggtgtcgc cccgctgcac
      361 ggtgtagtgg cgggccggtc cgcccttctt ttctttctca actggctgct tctttgtccc
      421 gctcgcggtt ttctccgtcg cgctcgccgc cttcttctcc gcccagcgtc cctcgatctg
      481 ctggcgcagg ttcagattct cgttgcgcaa acgcaccacc tccgagcgcg gcaccatcga
      541 gtcgccggtc agattcgtga gcagagtgag ttcgtcgcgt ttcatgaaac ccttcacctc
      601 ctcggcccgc ttcccgcccg gctcgagggc gaggaaacgc ttgaaatgat gcaacgcatt
      661 gagcggatca ttcagcttgt cgtcgtaaat gagggcgagc ttgtaatgca cgtcggccga
      721 atccggccgg ccatcgagag ccgcttcgta gagattgatc gcctcgacgt aattcccttc
      781 ggcccttttc gcgtctgcat ccttgcttag ctgcgcctgg cgctgtgtga tcatccgatc
      841 gcagccggca agcgcgccta acgagaacag aaagccaatc agaactcgta atcgtgctcc
      901 tgctcgggct cgtgatcgaa ttgggaaagc catcaattct tgagcacgat tacgatcacg
      961 agcaggagca cgaccggaaa ccctaatacc gcggcacatc gggatcgatc tcttccgacc
     1021 acgcatcgat cccgccggcc acgttcgcga ttttcggaaa tcccgctgca cgcaagaatt
     1081 cggccgcccg ttcgctccgc acgccgctgt gacaaaagac gaagatctcc ttctcccggt
     1141 cgagttcacc aaagcgcgcc ggcaactggc ctaacggaat caggttcgcc ccggcgatgc
     1201 gcgccagctc gaactcaaat ggttcgcgca catcgagcaa gatgtagggc tcgcccgagg
     1261 ccatcctctg ctccaaggcg cgcacctgaa tttgcggcac cgcggccgtc agatccggtc
     1321 ctccatggca gaacgtttcg taatcgatcg gcgcggtgat cgtcggttgc ctgccgcaga
     1381 ccgggcaatc gggatcgcgc cgcagattaa attcccggaa cttcattttc agcgcatcga
     1441 agtggacgag tcgcccgacg agcgtctcgc cgaggccaag aatcaacttg agcgcttcgg
     1501 tcgcctgcaa cgtgccgatc atgccgggga gcacgcccag cacgccggct tccgcgcagc
     1561 tcgggaccgc gcccggcggc ggcggttcgg gaaagagaca gcggtaacac ggcccgccga
     1621 gatgcggcgc gaagaccgtg gcctgtccat cgaagcggaa gatggaaccg tatacattcg
     1681 gcttgcgcgc gaagaccgcg acgtcgttgg agagataacg ggtggcgaaa ttgtccgtgc
     1741 catccacgat cagatcgtag tcgcgcagaa tcccctccgc gttcccgctc gtgaaccggc
     1801 aggcgtggag ctggaccgcg acatgcggat tggtctcgac caaacggtcg cgcgctgatt
     1861 ccgttttcgg gcggccgacg tctttggtcc cgtgaagaag ttgccggtgg agattggaaa
     1921 gcgcgacccg gtcgtcgtcc accagcccga tggtgccgac gccagccgcg gcgaggtaga
     1981 gcgccgccgg cgagccgagc ccgcccgtgc cgagacacaa aacgcgcgcc gctttcaggc
     2041 gctcctgccc agccacaccg atctccggca gcatgatgtg ccgcgaatag cgccggcgtt
     2101 cctcttcccc gagactcgcg cccgccttct cgttaggtga agaggtcgtt atgttttcac
     2161 cagctcccat ttcatcccaa cgatccgctg atccgcgaag cgcttcaagg atttcgaaaa
     2221 actgaggtac aacgcgttct gccgtgcgac gaagcccacg cagccaaatt ccgcgccgcc
     2281 cgcggagcgc tggatcgtca tgatgcgcga ttgccggatc tgcgcttgca agcgtcgatc
     2341 cttattcggc gcaagccaca gggtgtaaac ctgcggcgtg ccacaggcct cgaccacgtc
     2401 agtgaagcgc gcggtcctag tgttgaggag ttgcaaggtg gcgaacggat cgcgaaaaaa
     2461 cgaggcgttg gcaaggcgat gctggtttcc cccagacaga caggcaccac cctggctggg
     2521 tagcgcaccc gtctcgggtg ctggttttgg cgttccgccg aaacaggctt tccttggagc
     2581 gacgcaccaa tggaagttcg cgacggcggg acgccttcgc cagcacccga cacgggtgcg
     2641 ctacccagac atcagaatat ggtgaaaaaa agtccgccag aggaccacgg caaagaggat
     2701 cagactaccg ttgctgcatt cctgccctgg cggggttcgt tagtcctcag tccatagccc
     2761 ctgacggaga ggcaaggtcc ggcgaagccg aacgtcgcgc aatcagtttc tcggtcgcgt
     2821 tgacgcgcga gggtcgctcc ggttcgatgc ctctgccgcc ttcgccgtcg acccggacgg
     2881 gagaaggttg ccgccgcgcg tttcgcctaa cgaattagag cccgagcaac tcgcgctgcc
     2941 acgcgacgag cacggtctcg gcgcgactcg ggtcggccgc cagcgcttcg agcgcgctgc
     3001 gcggcgcgat gaacgaaggt tcgagtgcca gttccttcgc gtgctggtcc cgccggcgtt
     3061 tcagctcttc cactttcctg tcgaaatcgc gggtccgagc ccgattcacc cgcggcggcc
     3121 gttgcggcca ggccgattcc ggcaactcga gagcggcccg cgccgcggca agaaaaccgc
     3181 gccttcggcg ctcggataag tggcgaaatt ccggcgtctc accggccaca aattgtttcg
     3241 ccgcctcgat catcgcgctg ttttgcaaaa tgtggaatgc cggccgatcc gcgtcttttg
     3301 cctcgctgtc gcgccattgc cagatggcgc gcaacacggc gcccgtttgg ccgggcaacg
     3361 ttccgctgcc cgcgatccgc catgcttcat ccggatcgcg ctcccgcgtg accctcgtct
     3421 gctcgagcgc gcgctgacac gattgccgga accatcccgt ccggcccagc gcctccatct
     3481 gctcctccat ccgggccgcg agcgggagaa gaaaatgcgt gtcgttcttc gcgtattcct
     3541 ccatcgcgcg cgggagcgga cggcgcgccc agttcgcttt ctgcgaaccc ttggccaggg
     3601 tgacaccgaa gtattgctgg accagtgccg cgaggctgaa ttccgtcaac cccagcatgc
     3661 gcgcggcgat caccgtgtcg aaaacttcgc ggacatcaaa attgaaggag cgtcggagca
     3721 ggcgcagatc gaaatccatc ccctgcaaca cgatctcttt ttcggcgagc gcggtgcaca
     3781 atggctgcag atcgatcgac gcgagcggat cgaccaggta atcctggccg tcgaacgtga
     3841 gctgaatgag gcacagcttt tcaaaatagc agtgcaagct gtcagcctcg gtatcgaccg
     3901 cgacgcgcgc gaacgagcgg aaccgcggaa ggatctccgc gagttgtgcg tcggacgcga
     3961 tcaccgcagg cccgccagca aaagctccgc gttggttttg gtcgcgtgca ggttgttgat
     4021 caataattcc atcgcctcga gcggcggcag ggccgccatc gtcttgcgca acatcttcac
     4081 ccgctcccat tcctcgggat ggtaaagcag gtcttcgcgc cgggtgccgg attgcaggat
     4141 gtggatcgcc gggtaaagcc gcttctcgac cagcgaacga tcgagatgca actccatgtt
     4201 gcccgtcccc ttgaactctt caaagatcac ttcgtccatc cggctgccgg tctcgatcaa
     4261 agccgtcgcg aggatcgtta ggctgccgcc ttcctcgacg ttgcgcgccg cgccgaagaa
     4321 acgcttcggc ttgagcagcg cctttgcgtc gaccccgccc gacatcgtgc ggcctttgcc
     4381 gcgaatcagg gcattatatc cgcgcgccag gcgcgtgatg ctgtcgagca tgaggacgac
     4441 gtcttttttc tgctcgacca accgcttcgc ccgctcgctc accagctccg ccacttgcac
     4501 atggcgggtc ggcggttcgt caaaggtcga gctgtaaatc tggcaatcga cttcgcgctg
     4561 caggtcggtc acttcttccg ggcgttcatc gaccagcaga aggatgagca caatgtcggg
     4621 atgattcatc cggatcgctt tcgcgatctg cttgagcaaa atcgtttttc cgacgcgggg
     4681 cggcgcgacg atcaagccgc gctgtccgcg accgagtggc gtcagcagat cgaccgcccg
     4741 cacgcaggct gaatccgccg aatcgttttc cagcatgatg cgaccctgcg ggaagagcgg
     4801 cgtcagatgg tcgaaggggg tgggcgcctg ccacgcctcg gccggctggc cctcgatcga
     4861 gaggatgcga tccaacatca tccctttctc gcgctcgcgc ggcagccgga tcgcgccggc
     4921 gagcaattgt cccgggcgga gattgaactg gcgaatgagg tgatgcgcca cgccggcgtc
     4981 ctccggcaac gggagaaaat tcaactccgg cgaacgcagc atggcggggc cgtcgttagg
     5041 ctgttcgaga aagccgctcg tccggacctc ctggccccgc gacacggcgt gccggacgag
     5101 atcgaggatc aactgatgcc gcgtccggct cggatgcggt tgcaggtcga agcggacaaa
     5161 caaatcctgg agcgcggctg gcgccaggtc tcggagcgcg ctgagctcaa gcggctcgaa
     5221 cggtggcctg tctttttcct caggctcggg gagaagcgtg gccgggccgg cgttttccgg
     5281 cgctgtaaat ttttcttcgt ccatcacttg gcggccagga aacgcgccag gatcccggcc
     5341 tgcgcttcga gattgggcac ggggccatta ttccagacaa cgtgatcggc ccgggagatt
     5401 ttttgtggga gaggcatttg agagcaaatc atggcgtgag cgtcggaagg cgtgagaccg
     5461 gtgcgggaga tcagccgttt ttgctgaatt tccgacgcgc aggcgacaac caccacacgg
     5521 tcgcagagga cttcaccttt ggtttcatag aggagtggga tatcggccag gaaaatctcg
     5581 ccggactgac ggctttcttc cgcttcagtg gcccactgac ggcggatgcg cggatgcagg
     5641 atttgctcga gcgcgcgctt ctttccctgg tcggcgaaga ctatggcccg aagtgcggcg
     5701 cgattcaaat cgccgcccgc agaaaaaatc tcggggccga aacgctcgcg caagagccgg
     5761 cgcacttccg gatcctgctc gacgagttgc cgggccgcgc gatcggcgtc gaagggcttc
     5821 gcgcgcagca actggctgag acgattgcaa aaagtggact tgccggtgga gatgccaccg
     5881 gtgatcccga tgacgctgca ggattcaggc attacgaaag agagggtcgc ggaatggcaa
     5941 agcagggtcg atccgctttg caagagaact taaccgcggg caatggcaag ctcgcaattg
     6001 cggaccgtta ccgccccggg ataataccag gcaaagaggg cgctcccaag aactgggccg
     6061 cagtgattgc gcgcccccgg ctggggcgcc cggttgtcct aggagggttg gcgtcagagg
     6121 ggtgggacct ctgacgaaat ggagttcgaa gacgaacgtt cggatgcgcc gtttagctgc
     6181 gctcggccgc gtccgccgct gcggcgagag cgttgagcgt ggcgaaaaac tcagacgatt
     6241 ggccgatgaa aagccccatc gcggtcagga aattgaccac gcccatcgca atcccatctg
     6301 tttcggtcgg acgaataatc gagcacaccg ggagatttgg gctgatgaaa ttgcacggct
     6361 caggcatgat caggttcgtc tggaagttct gcccgccaaa gggcggacta ttaaggtcag
     6421 gaaagaccaa gccattggtg gggtcggtca aaggaggcgc gttcccggcc ttatccgccc
     6481 aggtttgaaa atgcatggtc tcagtgggac cgatgctgat gaggattc
//
LOCUS       DATMNS010000287        22992 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3819954, whole genome shotgun sequence.
ACCESSION   DATMNS010000287 DATMNS010000000
VERSION     DATMNS010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 22992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22992
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3819954"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            511..1947
                     /locus_tag="VNW28_09975"
     CDS             511..1947
                     /locus_tag="VNW28_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXA10300.1"
                     /translation="MPDGLNLYDVKEETVDTHSKALKINLDPRWYGTFAEIGAGQEVV
                     RWFFRVGGAAGTIAKSISAYDMKVSDAIYGHAGRYVSRGRLQAMLDREFDLDIERLAH
                     ERGDNTSFFAFADTVVARSFRGGNECHGWMGIKFQSRVRDEPSQIVMHVRMLDAEASL
                     QQEALGIVGVNLCYGAFFLNHVPEELVESLLDKLSTGRVEIDMLEFRGIEFRNVDNRI
                     MALKLVQLGLSGAAMFGADREVLQPAEVLHNKAVLVERGSFRPTTHVNLDMLECALAK
                     FKEDPAVADKEILPLMELTMRNLLAGGTEVDRRDFLARAELLAACGMTVLISDYFEYY
                     RLAAYLALRTRERIGIVMGVPSLYELFDEKYYAQLPGGTLESFGRLFKNDLKIYVYPL
                     RRAEGDELQTINTFDLKLDLKPLYDYLVRRGSFVQLDNFNPKYLPIFSRDVLKRIGGG
                     DESWDEMVPPQVATLIRERGFFGYTKKE"
     gene            2023..3081
                     /locus_tag="VNW28_09980"
     CDS             2023..3081
                     /locus_tag="VNW28_09980"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXA10301.1"
                     /translation="MKPALFILLALFALAVRVDAGWFDRHTPTPPPTPIPVRKALPPL
                     TPTPSPTPKPTPRRLVALPVYDEETSVRLQIFLDNNEFGPGKIDGRMGEFFGKALVAY
                     KKAHGLEPTGAVDAQLIAQVPEPYTNYTIRPEDENFVGEVASKPAEQAKLKAMKYGSL
                     LEFVAERYHAAEDFLRKINPEVDLEKLKPGDTVKVPNVQPFEIEKLSEKFIPANPAFA
                     TRQIFIDTRENFLEVRDGDKLLAEFPITPGSKTLPAPKGVWKILGIATLPWFRHDEGV
                     LNYGVRTGDFYNIPPGPNNPVGILWMGLNKPGIGIHGTNNPETIGRAGSHGCIRTANW
                     DAARLKDMVSPGNVVTIF"
     gene            3235..3786
                     /locus_tag="VNW28_09985"
     CDS             3235..3786
                     /locus_tag="VNW28_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXA10302.1"
                     /translation="MDSSEHDIYRAAIHGDREAFEMIIRMQSRALFAIAYGVLQNREE
                     AEDVVQDTLVKAWKTRWRVRDPKKFPAWLGTIARHRARDVARRRRSISFPADREEPID
                     PGNDATAAISRENEVGEQVRAALSTLPELHRAALTLRYFEAMDYASIERTLGLSNGAL
                     RGILGRALAAMRQRLRPALASLE"
     gene            3792..5813
                     /locus_tag="VNW28_09990"
     CDS             3792..5813
                     /locus_tag="VNW28_09990"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HXA10303.1"
                     /translation="MPNDTIHLEIERWLAADVHDQLSSDERAALEQHLAACPACRALQ
                     QEEKNMNQLLENTLAKESADPAFEQRMVGRFRNRTGARDGGLIPFFGGLMRMRAAQIT
                     AVAALLLTLVQVGKIVTGERAALPGRDAPMAFAPSGPREPIASTTAGAIATDSSVSRT
                     SAQAIEFGSTDSVQGKARSEVKKSLDDKFKVAPMTLAGAQMQQESDRPDKSGPSAESP
                     ALPSAAPSDEGRKLIRNAQLDLEVTTYEGTIRRLTTFATEEHGFVATQSSAKLPNGKL
                     QGTIVIKVAPDNLDRFLEKARGLGELKNQTLGTDDVTKAYFDTDARLRNAKRMEERLL
                     DMLQKKTGKVSDLLEVEKELGRVREQIEQMQGTLKYYDALVQDATVTITLAEKDLNEP
                     AAFLLRETANLSLFSADVEKTFGEVKSALQNAKTQIVQSNLERDSSGQATARMTLLIA
                     PEESDAVIARIKGMGRVQNFNVQTERVAQNGSGMSNTAKVERDKVQLNLVIARTGEEQ
                     SVQQTSLRIITSAVMDKVAQLKTAVSKAGGELRSSTFSRNSDGQEIANITLRVPMKNY
                     SSLVASFDHLGEVKDLTVQRNDQPGTTANPETAPADVSLQIYSPGKIVADDTGLTATI
                     RRTLGQGAAALMWSARMIGVALAFLAPWAFAVGLLGWLVVRFAKRRGSR"
     gene            complement(5925..6563)
                     /locus_tag="VNW28_09995"
     CDS             complement(5925..6563)
                     /locus_tag="VNW28_09995"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXA10304.1"
                     /translation="MSFDRVAPHYRWLETVAFGQQLQQARLAFVRRLEAPRRALVVGE
                     GNGRFLAALLREDRAVRVDCVEASGRMIALARTELDVESRVNFIHAEIQTVKLVAQRY
                     DLIVTHFFLDCFSKETLAAVIAKLANSAAPQAQWLVADFCQPRQGWRSLCPRLLIAMM
                     YLFFRILAGIEARRLVDYRPFLRAEGFILAQETLSPNKMIRSELWRHTLSTG"
     gene            complement(6560..7447)
                     /locus_tag="VNW28_10000"
     CDS             complement(6560..7447)
                     /locus_tag="VNW28_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10305.1"
                     /translation="MPNAPELVAAPSSPLAAQAGYSPIIWLNLLCLDAPIVALSWQWL
                     FARSFGAHLTFPLRALLFLTAWLIYLADRFADTIRLPSAVPMSLRHRFCKAHSVGWWI
                     AIVVIFVVDTALAVRTLDLQVLLLGGTLATLCALYLFVNHSLGGRWGPFPMKEKAVGV
                     LFAAGTTFAVVAVLPSLTISFAVALALFALLCTLNCLLIAAWERELDAAQGKSSLLTG
                     WPAAEGVFKPLGLVLAGATLVIAVFWRFAAPLWLCLAASALLLVWLNGATHLERDRRT
                     ALADLVLLTPLAVLLLAFR"
     gene            7641..9380
                     /locus_tag="VNW28_10005"
     CDS             7641..9380
                     /locus_tag="VNW28_10005"
                     /EC_number="1.14.16.-"
                     /inference="COORDINATES: protein motif:HMM:NF010657.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0009072 - aromatic amino acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid hydroxylase"
                     /protein_id="HXA10306.1"
                     /translation="MKTESGIPMNNAAVAALPRHLLQFAVDQRHDEYTSVDHAVWRFI
                     MRQNVFFLKEYAHKVYFQGLLETGISFERIPRISEMNDILGRIGWGAVAVDGFIPPAA
                     FMEFQAYKVLVIACDMRQIHHIEYTPAPDIVHEAAGHAPIIVDREYSNYLQRFGEVGA
                     KAMSSKKDFELYQAIRHLSILKELPNPDPKELAEATSKVEHGQKNLGQPSEMALLSRL
                     HWWTVEYGLIGALEAPKIFGAGLLSSIGESVSCLEPHVKKIQYSIAAAETAFDITTKQ
                     PQLFVCRDFQHLSDVLEEFASRMAFRVGGLEGINKAIDSANPSTCEYSSGLQVSGVFT
                     EVLTDADGKPIYLRTTGPSALAFQGKELPGHGRDYHRDGFGSPIGGWKETEIREGQRA
                     RLEFESGVTVEGKIEKVLGRADRIQLITFSNCTAKIGDRLLFDPAWGTYDMAVGEKIV
                     SVFNGAADKDAYNQVALVPKERTIKCPSDARRKKLESLYQQVRDIRTHKTGSERLGEI
                     WETQQAEHPNDWLLSMEIFEILDELDEQKPLKEKIAQFLSARKAVNKEIATLIEWGFR
                     LVTYHQQDTRAAV"
     gene            9410..9742
                     /locus_tag="VNW28_10010"
     CDS             9410..9742
                     /locus_tag="VNW28_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HXA10307.1"
                     /translation="MPQREHYLFVCTNRRADEDPRGSCTARGAEDVRQALKDQISRQG
                     LANLKARVCASSCLDQCSSGVTILVEPDHFFYGRVTVADVPEIVDGIVQGRRVERLLL
                     TEQDLAKG"
     gene            9807..9962
                     /locus_tag="VNW28_10015"
     CDS             9807..9962
                     /locus_tag="VNW28_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10308.1"
                     /translation="MKQILLLLCAVAVFFESGCATRPSINEQSLQDQSDDQAKKRSDD
                     FARGLAQ"
     gene            10024..10266
                     /locus_tag="VNW28_10020"
     CDS             10024..10266
                     /locus_tag="VNW28_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10309.1"
                     /translation="MTNNLMIRRLIQLGALCLIGDGVMGLLKPRWHSLLWHCGPQIAR
                     AATEELAEHPNTARAVYLAEAAIGVALASWQTPEGE"
     gene            complement(10390..10785)
                     /locus_tag="VNW28_10025"
     CDS             complement(10390..10785)
                     /locus_tag="VNW28_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10310.1"
                     /translation="MNTSLLLSLGFAGLFLGFAQAQTQPASTPDNSTGLEGTISLSPA
                     RGGPTRQGISDSKPATNMAFEVKQGGRVIGAFQTNDLGEFRVSLKPGHYTISRKDWKV
                     SIGSYGPFEVDVGEGKMTNVHWDCDTGLR"
     gene            12065..12418
                     /locus_tag="VNW28_10030"
     CDS             12065..12418
                     /locus_tag="VNW28_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10311.1"
                     /translation="MANPTGKAFSRRAPDIITEELVAVFASKASFEFKPLFDVVFANL
                     RARNAASGGEEMLRLRSYEKLQSLVDRGAVKKTIAGTVKKYKGVSAPLTVLNTELQQT
                     RLAWSQRAVGSAAAA"
     gene            complement(12763..13047)
                     /locus_tag="VNW28_10035"
     CDS             complement(12763..13047)
                     /locus_tag="VNW28_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10312.1"
                     /translation="MKLTTTCGMIAAGVLAFASATDAGSTKSYQVTGPVLEMNDTMIA
                     VQKGKERWEVARDASTKINGDVKVGSKVTVMYTMTATEVDAKGEKAAKKP"
     gene            13163..13492
                     /locus_tag="VNW28_10040"
     CDS             13163..13492
                     /locus_tag="VNW28_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10313.1"
                     /translation="MIPVDPERSENAPSDARNLAAQPIRRLRTYNFFASIAPEQKSPA
                     NGSTANHKIAAPSIRKGSTKNAFGPMRRETSGNVDLPGGRNINLILNGSHGRALFPAG
                     IDPVNVT"
     gene            14214..15347
                     /gene="solA"
                     /locus_tag="VNW28_10045"
     CDS             14214..15347
                     /gene="solA"
                     /locus_tag="VNW28_10045"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF008425.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HXA10314.1"
                     /translation="MRFDVAVVGLGGMGSALLAQCAQRGASVIGLEQFARGHELGSSS
                     GKSRMIRKAYFEDPAYVPLLRRAYDLWRELERATGAELLRRTGLLMVGEADCEIIAGA
                     GRAAREHGLPLESLTAREIRERYPMLTVLDGEVGVYESDGGVLDPERAVEAHLVAAAT
                     AGAQMRFGVAVQNWEATREGFAIHLADGTSVASRALILSLGPWFKDALERIGISIRVQ
                     RNVQAWFEPATEAYAAPHFPPFLLDRKGLPAPLYGFPDFGDGVKAAFHSYGDLTDAAN
                     VVREVDLARDIAPIVHAMEQWMPGAALKLRAAKPCLYTLTPDCHFVVDRHPEHSRLIL
                     CGGFSGHGFKFAPVIGEIGADLALTESSRHKIDFLSLRRFARK"
     gene            15475..16425
                     /locus_tag="VNW28_10050"
     CDS             15475..16425
                     /locus_tag="VNW28_10050"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004364 - glutathione transferase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HXA10315.1"
                     /translation="MAIKAQFAAEQSATGEFERQDDAFRDWVSADGSTPYPAVAGRYH
                     LYVSLACPWASRTVIVRLLEGLEEAIGLTVVDPVRDERGWAFRDGPGYSRDPVNDFSF
                     LREAYAATDPAWDGRVTVPVLWDTETHRIVNNSEDDICRMFNGAFRALGRRSVELFPN
                     DIAQEQAELSAFIYDRVNNGVYKAGFAIRQRAYEHACRALFAALDQLEIRLTTSRYLF
                     GDRIVESDWRLFCTLVRFDIVYYIHFKCSLRRILDYPNLQSYLMDLFQQPGIAPTVNF
                     EHIKHHYYMTHDAINPTRIVPIGPLLDLMRPHGREKLSAP"
     gene            16545..17405
                     /locus_tag="VNW28_10055"
     CDS             16545..17405
                     /locus_tag="VNW28_10055"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HXA10316.1"
                     /translation="MADIHISRSGTTLGVFPEEEVREGLKTARFAPTDLGWREGMSTW
                     LPLAQFPEFATAGAPPAATGALPSASVPGAPGARTGLPWDRRRELGLVNAFIETLKLV
                     LLNPTVAFSAMQTEGGLAEPLIYAVIGGSAGLIVYFLFSIFFGSFGIMSHRNPLAGIM
                     GFGIGGIFFVIFVPVLLAIGLFIGGAILHLCLTLVGGARRSFETTFRVLCFAAGSTYP
                     LMIIPLCGGLISGIWCIVLQCIGLARAHDTTTAKAALAVFLPLVVCCGGGLILGLTFG
                     TLGALFGHHH"
     gene            17418..17837
                     /locus_tag="VNW28_10060"
     CDS             17418..17837
                     /locus_tag="VNW28_10060"
                     /inference="COORDINATES: protein motif:HMM:NF022281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2752 domain-containing protein"
                     /protein_id="HXA10317.1"
                     /translation="MKAYRRPLAPREIDHELLWLVVSGATFAGLAFWLAARLPTPQCA
                     FRSLTGLPCVTCGATRAAIQFFHGHFAASFLFNPLAFLTLCSLLAFDLYALTVLIARA
                     PRVRLGNFSRAEKRLARSVAIILLAGNWIYLLTAHPF"
     gene            complement(17945..19045)
                     /locus_tag="VNW28_10065"
     CDS             complement(17945..19045)
                     /locus_tag="VNW28_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HXA10318.1"
                     /translation="MESASTGQLILTGVPGTELDGKTAALFRKVQPGGFILFGRNIES
                     AGQLRKLIDDLRDLSALEPIITIDQEGGRVSRLRLIGQEPPNAQQLREKGDLELVRRH
                     GDITGRLLRLFGFNLDLCPVLDIAFDDEADNSLRGRCYGRDVAETVWLAGAFNDALRR
                     QGIASCGKHFPGYSAATVDAHHDLPVIERTRAELDANELAVFRALIDRVDSMMICHGW
                     YPTLEAEKTAASLSRKIVTELLRDELGFVGLVMTDDLDMGAILKEYGFEETIRRAIGA
                     GNDLAMICHRVPAIAEAHGYLEKLPRADLDRALANIARFKENLAPPHEFSESTFASLN
                     AEIWDLRVAVLGEARAKVPSPEDGKRSPVETY"
     gene            19066..19308
                     /locus_tag="VNW28_10070"
     CDS             19066..19308
                     /locus_tag="VNW28_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10319.1"
                     /translation="MHDTRNKKWFVRLAALLSRAASAQGSVELGIDVLASHDYSLLAG
                     KRVGLSTNRTGVNSAGIASWRPNEQEFRTRAQDLLY"
     gene            19317..19901
                     /gene="nadD"
                     /locus_tag="VNW28_10075"
     CDS             19317..19901
                     /gene="nadD"
                     /locus_tag="VNW28_10075"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HXA10320.1"
                     /translation="MKKIGIYGGSFDPIHHGHLILAREALENLDLAEVIFVPAAQSPH
                     KLARPAAGAAVRWEMLSVAIAHEGGFSASRLEIDRPPPSYSIVTVEQLRAKKTNAEFF
                     FLVGEDNLPQLPTWHRFNELRQLVQFVVLDRSGVVLDDNYPVVRRKIDISATTIRNRV
                     ASGQSIRYLVPEAVEQIIRRENLYQGANRSHLKS"
     gene            19883..20236
                     /gene="rsfS"
                     /locus_tag="VNW28_10080"
     CDS             19883..20236
                     /gene="rsfS"
                     /locus_tag="VNW28_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HXA10321.1"
                     /translation="MTPEKLAKTCAELAADKKAEGVVVLDLREISTFTDFFVICSGTS
                     EPHLKAIAGEIETRLRQDHKIKPAAVDGFPASQWIVLDYLQVIVHVFHQEKRDFYSLE
                     DLWSDAPRLTIAGVA"
     gene            complement(20247..21071)
                     /locus_tag="VNW28_10085"
     CDS             complement(20247..21071)
                     /locus_tag="VNW28_10085"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF008602.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXA10322.1"
                     /translation="MKKLPLIAVVVFALAPLAPAADPQLKDQKDKVSYSIGLDIGTTL
                     KRQLIDVNQDLLSNGIKDGLSGKKPLLTDEEVKATMATFQKDMMAKQAATKKAAGEKN
                     AAEGTKFLEENKKKEGVKTTASGLQYKVIKEGSGPSPKAVDAVKVNYRGTTIDGTEFD
                     SSYKRGEPATFPVNRVITGWTEALQLMKVGSKYQLFIPPNLAYGERGAGSDIGPNSTL
                     LFDVELLGITPAGTTPTPKPAAYDSSRPTGQPGAAIPPKLPKPAASPAATASPQKP"
     gene            21238..21984
                     /locus_tag="VNW28_10090"
     CDS             21238..21984
                     /locus_tag="VNW28_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10323.1"
                     /translation="MLDVSAAVSLSHEDAVRIGHRIWQNECGGTVAGLTSWNAGENFA
                     SLGIGHFIWYPAGKRGPFEESFPEFVRYAAQRGAKLPKLLLANGGDVCPWPSRAEFLA
                     AESGGKMETLRQFLVDTIDLQADFLVNRMQAALPKMLAAASPAERGKVAQQFNRLASS
                     APGCYALIDYVNFKGEGVLLSERYQGRGWGLLQVLEEMSGSGDATAAVNEFADAATRV
                     LRVRVENAPRERNEARWLPGWLNRIDSYRY"
     gene            22226..22876
                     /locus_tag="VNW28_10095"
     CDS             22226..22876
                     /locus_tag="VNW28_10095"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HXA10324.1"
                     /translation="MKTPDPLIFVIARLSRLSVPLLLALLALANVNAQLSRPFARPQP
                     HATPPMKRASELINRQEPINVSPRLLARLTPDNSHVRVSISKQRAYLMMGDEIVIDSP
                     ISSGKRARPTPQGSFHVLEKDLNHKSTIYGDFVDSSGRVVRRGVSSRIDSAPSGTHFV
                     GADMKWFMRLTDEGVGMHVGFLPGYAASHGCVRMPSSAAEMFYNHVKVGTPVVVGD"
BASE COUNT         4647 a         7002 c         6971 g         4372 t
ORIGIN      
        1 agagtttcgt gtaacgaaag agcggttcca gactacgcga ggtaaatggc tccgagggct
       61 agagcctgtc atgcattttg caacagagag ttaagagtgc gtgtcccgca tttcgcgagc
      121 caggccaagg gggagctgta gccgcttcgc tgtgcgaagc gtggggtatg tgtgtggcaa
      181 aggggattga cccatcgcac ccgcgtcgcc tacggggcga cagctagagg gttgcgcgtg
      241 gaacgcgtga ggagggcatt cccgcgcagt tgtagctcct tcgctttgcg aagcgtgggg
      301 tatgtgcgcg gcaaggggac tcattcatcg cacccaagtc gcccacgggg cgacggctat
      361 aggacgtgga ttttgctggt cgcgcaaacc cggaagtcca tgacggcctc tagagcgagc
      421 gacggggact tcggctggca gcaactgaac gcttctcaca tttgcacgcg tctcccgtcc
      481 aaattccgag aacgaaagtc tcgcgttttg ttgccggacg gcctaaatct ctacgacgtg
      541 aaagaggaaa cggttgatac gcattcgaag gcgctgaaga tcaatctcga tccgcgctgg
      601 tacgggacgt tcgcggagat cggggccggg caggaggtgg tgcgctggtt tttccgcgtt
      661 ggcggcgcgg cggggacgat cgcaaagagc atctcggcct acgacatgaa ggtgagcgac
      721 gccatctatg ggcacgcggg gcgctatgtc tcgcgcgggc ggctccaggc gatgctcgat
      781 cgagagttcg atctggatat agagcgcctg gcgcatgagc ggggcgataa cacttcgttc
      841 ttcgcctttg ccgatacggt agtggcgcgc agctttcgcg gcgggaacga atgccacggc
      901 tggatgggga ttaaattcca gagccgggtg cgcgatgagc cgagccagat cgtgatgcac
      961 gtgcgaatgc ttgacgccga ggcctcgctg caacaggaag cgctcggcat cgtgggcgtg
     1021 aatctctgtt acggcgcgtt tttcctaaac catgtgccgg aagaactggt cgagtcgctg
     1081 ctcgacaaac tctcgacggg ccgggtcgag atcgacatgc tcgaatttcg cgggatcgaa
     1141 tttcgcaatg tggacaaccg catcatggcg ctgaagctgg tgcagctcgg gctgagcggc
     1201 gcggccatgt ttggggccga tcgcgaggtg ctgcaaccgg cggaggtctt gcataacaag
     1261 gcggtcctgg tcgaacgcgg aagtttccgc ccgacgacgc acgtcaatct cgacatgctg
     1321 gagtgtgcgc tggcaaaatt taaagaggat cccgcggtcg ccgacaagga gattctgccc
     1381 ttgatggaac tgaccatgcg caatctgctc gccggcggca ccgaggtgga ccggcgcgat
     1441 ttcctcgcgc gcgcggagct gctcgcggcc tgcggcatga ccgtgctcat ttccgattac
     1501 ttcgaatatt accggctcgc cgcctacctc gccttgcgga cgagggagcg catcgggatc
     1561 gtcatgggcg taccgagcct ctacgagtta ttcgatgaaa agtattacgc gcaactgccc
     1621 ggcggcacgc tggagagctt cgggcggctg tttaagaacg acttaaagat ctacgtttat
     1681 ccactgcggc gcgctgaagg ggatgagttg caaaccatca acacgttcga cctgaaactc
     1741 gatctcaagc cgctctacga ctatctcgtc cgtcgaggca gtttcgtgca gctcgataat
     1801 ttcaacccca agtatctgcc gatcttctcg cgcgacgtgc tcaagcgcat cggcggcggc
     1861 gatgagagct gggatgaaat ggtgccgccg caagtcgcga cgctcatccg cgagcgcggc
     1921 tttttcggct acacgaaaaa ggaatgaccg cgcgccgggc cgggcgcggt cgaggtttgc
     1981 ggcggttgca tgcgcgcggc gaacggtcga taacttccgc tcatgaaacc ggcgctgttc
     2041 attttgctcg cgctttttgc cctcgccgtg cgcgtggacg caggctggtt tgaccgccac
     2101 acgcccactc cgccgcccac gccgatcccg gttagaaaag cgcttccccc gctgaccccg
     2161 acgccctctc cgacgccgaa gccgacgccg cgccggctgg tcgcactgcc tgtttacgac
     2221 gaggaaacga gcgtgcggct ccagattttc ttagacaaca acgagtttgg tcccgggaag
     2281 atcgacggac ggatgggcga atttttcggg aaagcactcg tggcctacaa aaaagctcac
     2341 gggttggaac cgacgggcgc ggtcgacgcg caactgatcg cacaggtgcc cgagccctac
     2401 acgaattaca ccatccggcc ggaggatgag aattttgtcg gcgaagtcgc ctcgaaacct
     2461 gccgagcagg cgaagctcaa ggcgatgaag tatgggtcgc tgctcgagtt tgtcgccgag
     2521 cgctatcacg cggcggagga tttcctgcgc aagatcaacc cggaggtgga tctcgagaaa
     2581 ctcaagccgg gcgacaccgt gaaggtgcct aacgttcagc cgtttgagat cgaaaaactg
     2641 tcggagaaat tcatcccggc caatcccgcc ttcgccaccc gacagatctt catcgacacg
     2701 cgcgaaaact tcctcgaggt gcgcgacggc gacaagctcc tggcggaatt cccgattaca
     2761 cccggctcca agacgctgcc cgcgccgaaa ggcgtctgga aaattctcgg catcgccacc
     2821 ctgccctggt tccggcacga cgaaggcgtg ctcaattacg gggtgcggac cggcgacttc
     2881 tacaacatcc cgcccgggcc taacaatccg gtcggaatcc tgtggatggg attgaacaag
     2941 ccggggatcg gcattcatgg cacgaacaac cccgaaacga tcggccgtgc cgggagccac
     3001 gggtgcatcc ggacggcgaa ttgggacgcg gcacgcctga aggacatggt cagccccggc
     3061 aacgtcgtca caattttcta gcgtcatccc aagcaggtcg agggatcccg gaacgcgacg
     3121 ggaagcacct ccttgccatc gagcttttca gcgccggttt cgggcttttg gcggcgcgcg
     3181 aaaaatattc cgcgcggatt cgaacgtacg cccgtctagg ctttagatcg accgatggat
     3241 tccagcgagc acgacatata tcgagcggcc attcacggtg atcgcgaggc ttttgaaatg
     3301 atcatccgca tgcagagtcg agcgctcttc gccattgcct acggcgtcct gcaaaaccgc
     3361 gaggaagccg aagacgtggt ccaggacacg ctggttaaag cgtggaaaac ccgctggcgg
     3421 gtgcgcgacc cgaagaaatt tccggcctgg ctgggcacga ttgcgcggca ccgtgcccgc
     3481 gacgtcgccc gccggcggcg cagcatctcc ttcccggctg acagggagga gccgatcgat
     3541 cccggcaacg acgcaacggc ggcgatttcg cgagaaaatg aggtgggcga acaggtgcgc
     3601 gccgcgctct cgaccctgcc cgagctgcac cgcgccgcgc tcaccctgcg ctatttcgaa
     3661 gcgatggatt acgcgtcgat cgaacgcacg ctcggcctga gcaacggcgc gctgcgcggc
     3721 attctcggcc gcgcgctggc ggcgatgcgc cagcgcttgc gcccggccct cgccagtctg
     3781 gagtagcccc tatgcctaac gacaccattc accttgagat cgaacgctgg ctggcagccg
     3841 acgtgcacga ccagctctcc agcgacgaac gcgcggcgct ggaacaacat ctggccgcgt
     3901 gcccggcatg ccgcgctttg caacaagaag aaaaaaatat gaaccaatta ctcgaaaaca
     3961 cgcttgccaa ggaaagcgct gacccggcgt tcgaacagcg aatggtcggc cgtttccgca
     4021 accggaccgg cgcgcgtgat ggcggactga ttccgttctt cggcggactg atgcgcatgc
     4081 gagcggcgca gatcaccgct gtcgcggcgc tccttctcac gcttgtgcag gtggggaaaa
     4141 tcgtaacggg agagcgcgct gcgctgcccg ggcgcgacgc gccaatggct tttgcgccat
     4201 cggggccgcg ggaaccaatc gcgagcacca ccgcgggagc aatcgcgacc gactccagtg
     4261 tttcgaggac gtccgcgcag gcgattgaat ttggatcgac cgattccgtg cagggcaaag
     4321 cgcgtagtga ggtgaagaaa agcctcgacg acaaattcaa agtggcgccg atgactttgg
     4381 cgggcgcgca gatgcaacag gagagcgacc ggccggacaa atcgggcccc tccgcggaga
     4441 gcccggctct tccctcagcc gccccgtctg acgaggggcg aaaactaatc cgcaacgcgc
     4501 agctcgatct cgaggtcact acctatgaag ggacgatccg acgcctgacg accttcgcga
     4561 cggaggagca cggcttcgtc gccacccaga gctcggccaa attgcctaac ggaaagttgc
     4621 agggcacgat cgtgatcaag gtcgcgccgg ataatctcga tcgctttctg gagaaggcgc
     4681 gcgggctcgg cgaactgaaa aatcagaccc tgggcacgga cgatgtgacg aaagcctatt
     4741 tcgacaccga cgcgcgcctg cgcaacgcca aacggatgga ggaacgcctc ctcgacatgc
     4801 tgcagaagaa gaccggcaaa gtgtccgatt tgctcgaggt ggaaaaggag ctggggcggg
     4861 tgcgcgagca aatcgagcag atgcagggca cgctgaagta ttacgacgcg ctcgttcagg
     4921 acgcgacggt caccatcacg ctcgcggaaa aagatttgaa cgagcccgcc gccttcctcc
     4981 tccgcgagac ggccaacctc tcgctctttt ccgccgacgt ggagaagacc ttcggcgagg
     5041 tcaaaagcgc gctgcaaaac gccaagacgc agatcgtgca atcgaacctc gagcgcgaca
     5101 gctccggcca ggcgaccgcg cgcatgacgc tcctgatcgc gccagaagag agcgacgccg
     5161 tgatcgcccg gatcaaagga atgggccgcg tgcaaaactt caacgtgcaa accgagcgcg
     5221 tcgcgcaaaa cggatcgggc atgtcgaaca cggcaaaagt cgagcgcgac aaagtgcagc
     5281 tcaatctcgt catcgcgcgc acgggcgagg aacagtcggt gcaacaaacc agcctgcgca
     5341 tcatcacgtc ggcggtgatg gacaaggtag cgcaactcaa aaccgcggtg agcaaagcgg
     5401 gcggcgaact gcgcagttcg actttttcgc ggaattccga cggtcaggaa atcgcgaata
     5461 tcacattgcg cgtcccgatg aaaaactatt cgagcctggt cgcgtcgttc gatcatctcg
     5521 gcgaggtcaa agacctgacc gtccagcgca acgatcagcc gggaacgacc gccaaccctg
     5581 agaccgcgcc ggctgatgtc agcctgcaga tttacagtcc ggggaagatc gttgccgacg
     5641 acaccggcct gaccgccacg atccgccgga ctctcggtca gggggcggcg gccctaatgt
     5701 ggagcgcgcg gatgattggc gtggcgctcg cttttctggc accctgggca ttcgccgtcg
     5761 gattgcttgg ctggttggtc gtgcgcttcg cgaaacggcg aggcagtcgt taggcgtctc
     5821 gcgtgctggt ttggtgtctc accaaaatga tctcttcttg cgtccgccag aggaaaaaag
     5881 ttcgcgacgg tggaacaccg tcgcccgcac gcgagtcgcg tgcgctaccc ggtcgagagg
     5941 gtgtggcgcc aaagttcgga acgaatcatc ttgttaggcg aaagggtttc ttgcgcgaga
     6001 atgaagcctt ccgcccggag aaacgggcgg taatcgacga gacggcgcgc ttcgattccg
     6061 gccagaatgc gaaaaaacaa atacatcatg gcgatgagca atcgggggca cagcgagcgc
     6121 cacccttgcc ggggctgaca aaaatcggcg actaaccatt gggcctgcgg ggcagcggaa
     6181 ttggccagct tggcgatgac cgcggccaac gtctctttcg aaaagcaatc gagaaaaaaa
     6241 tgcgtcacga tcagatcgta acgctgtgcg acaagcttga ccgtctggat ctccgcgtgg
     6301 atgaaattta cccgggactc aacatccagt tccgtgcgcg cgagggcgat catccgcccg
     6361 ctcgcttcga cgcaatcgac gcggacggcg cggtcttcgc gcaggagggc ggcgagaaat
     6421 cgcccgttcc cctcgcccac cacgagcgcg cggcgcggcg cttctagtcg cctaacgaac
     6481 gcgagacgcg cctgttggag ctgttgcccg aaggcgacgg tctcgagcca gcggtaatgc
     6541 ggcgcgacgc gatcgaaact caccggaatg cgagcagcaa aacagccagt ggcgtgagca
     6601 ggacgaggtc cgccagggcg gtgcggcggt cgcgttccaa atgcgtcgcg ccgtttagcc
     6661 agacgagaag gagcgcgctg gccgcgaggc aaagccagag gggcgcggca aaacgccaaa
     6721 aaacggcgat gaccagagtc gcgcccgcaa gcactaatcc aagaggcttg aagacgcctt
     6781 cggccgccgg ccagcccgtg aggagcgagg attttccctg cgccgcatcg agctcgcgct
     6841 cccaggcggc gatcaagaga cagtttagag tgcagaggag cgcgaaaagc gcgagcgcca
     6901 cggcgaaaga gatcgtcagg ctgggcagga ccgccacgac ggcgaacgtc gtgccggcgg
     6961 caaagagcac cccgaccgcc ttttccttca tcggaaaagg tccccatctt ccgcctaacg
     7021 agtgattgac gaagaggtag agcgcgcaga gcgtcgccag agttccgccg agcaggagca
     7081 cttgcaggtc gagcgtgcgg acggcgagcg ccgtgtcgac gacgaaaatg accacgattg
     7141 caatccacca cccgacgctg tgtgccttgc aaaaacgatg ccggagcgac atgggcacgg
     7201 ctgacggcag acggatggta tcggcaaagc ggtcggccag gtaaatgagc caggcggtaa
     7261 ggaagagaag cgcgcggagc gggaaggtga gatgcgcgcc gaagctgcga gcaaagagcc
     7321 attgccagct gagcgcgacg atgggggcgt cgaggcagag gagattcaac cagatgatgg
     7381 gcgaataacc ggcctgagcc gcgagcggtg acgaaggcgc ggccaccagt tccggcgcgt
     7441 tgggcatcgg cgaacctatc cagcgttgcc gagtttcgca atcggccgat gcctcacgga
     7501 ctcgcccacc ccgggagcga cgcctcgccg tcttcccctg gatcgaatcg tgcttgcccg
     7561 tcttctagtt cagccataaa atctagaaca gggcgcgatc gttagggagc gcacaatttg
     7621 ccgggaccgg agctgttgcg atgaaaactg aaagcggaat tccgatgaac aatgcggcgg
     7681 tagccgcact gcccaggcat ctgctccagt tcgccgtcga ccagcgccac gacgagtata
     7741 cgtcggtcga tcacgccgtc tggcgcttca tcatgcggca gaacgttttc ttcctgaagg
     7801 aatacgcgca caaagtctat ttccagggct tgctcgaaac cggcatttcc ttcgagcgca
     7861 tcccgcgcat cagcgagatg aacgacatcc tgggcaggat cggctggggc gcggtggcgg
     7921 tggacggctt tattccgccg gcggcgttca tggaattcca agcttacaag gtgctcgtga
     7981 tcgcgtgcga catgcgccaa attcatcaca tcgagtacac gcccgccccc gacatcgtgc
     8041 acgaagccgc cggccacgca ccgatcatcg tcgatcggga atattcgaat tatttgcagc
     8101 gcttcggcga ggtgggcgcc aaggcgatgt cgtcgaagaa ggatttcgag ctctatcagg
     8161 cgatccggca tctctcgatc ctgaaggaac tgccgaatcc cgacccgaag gagctggcgg
     8221 aagcaaccag caaagtggag cacgggcaaa aaaacctggg ccaaccttcg gagatggcgc
     8281 ttctttctcg cttgcactgg tggacggtcg aatacggcct gatcggcgcg ctggaggccc
     8341 cgaaaatttt cggcgccggt ctgctctcct cgatcggcga gtccgtgagc tgtctcgagc
     8401 cgcacgtgaa gaaaattcag tattcgatcg cagcggcgga gacggccttc gacattacga
     8461 caaagcagcc acaacttttt gtctgccgtg attttcagca cctgagcgat gtgctcgagg
     8521 agttcgcgag caggatggcc ttccgcgtgg gcgggctcga agggatcaac aaggcgatcg
     8581 attccgccaa tccttcgacc tgcgaatact catccggctt gcaagtctcc ggcgttttca
     8641 ccgaagtgct cacggatgcg gatgggaagc cgatttatct ccggacaacc ggaccaagcg
     8701 cgcttgcatt tcagggcaag gaattgccgg ggcacggacg cgattatcat cgggacggct
     8761 tcggttcgcc gattggaggc tggaaagaaa ccgaaattcg cgaagggcag cgagccagat
     8821 tggagtttga aagtggtgtc acggttgaag gaaaaatcga aaaggtcttg ggccgcgctg
     8881 acaggattca gctgataaca ttctcaaatt gcaccgcgaa aatcggcgat cgccttctgt
     8941 tcgatccggc gtggggcact tacgacatgg ccgtcggcga aaaaatcgtc tcggtcttca
     9001 atggcgccgc ggacaaggac gcctacaatc aggtcgcgct cgtcccgaaa gaacgcacaa
     9061 tcaagtgtcc gtcggacgcg cgacgcaaga aactcgaaag cctttaccag caggtgcgcg
     9121 acatccgcac ccataagacc ggctccgaac gcctcggcga gatttgggaa acgcagcagg
     9181 ccgagcaccc taacgactgg ctgctctcga tggagatttt cgaaatcctc gatgagctcg
     9241 acgagcaaaa gccgttaaag gaaaaaatcg cgcagttctt aagcgcgagg aaggcggtca
     9301 acaaggagat cgcgacttta atagagtggg gcttccggct cgtgacctat catcagcagg
     9361 acacgcgagc cgcggtctag gcaagcgtcc caccgcccga atctgccata tgccgcagcg
     9421 cgagcactat ctctttgttt gcaccaaccg ccgggccgac gaggatcccc gcggatcgtg
     9481 caccgcccgc ggcgcagaag acgtccggca agctttgaag gatcagatct ccaggcaagg
     9541 cctcgccaac ctaaaggccc gtgtctgtgc cagcagctgc ctcgaccaat gttcgagcgg
     9601 cgtgacgatc ctcgtcgagc ccgatcattt tttctacggc cgcgtgaccg tggcggacgt
     9661 gccggaaatc gtcgatggaa tcgtgcaggg acggcgagtt gaacgcttgc ttctcacgga
     9721 gcaagatttg gcgaaaggtt aaggaaatcg cctctgaatt cggagtagat tgggatcccg
     9781 atgcgaggca cgttaccgat tattttatga aacaaatcct gttactcctc tgcgcggtcg
     9841 ctgttttctt cgagagcggg tgcgcgacac ggccgagcat caacgaacaa agtctccagg
     9901 atcaaagcga tgaccaggcg aaaaaaagga gcgatgactt cgcccgtggt ctcgcgcagt
     9961 gatgggcggg ggccaaggcc acgaattgtt tgaaaatttc cggagcacac tcgatagaat
    10021 ttgatgacca acaacttgat gatccggcgc ctgattcaac tgggtgcgct ctgcttgatc
    10081 ggcgacggtg taatggggct tttgaagccg cggtggcatt cactgctctg gcattgtggt
    10141 ccgcagatcg cccgcgccgc gactgaagaa ctagccgaac atccaaacac cgcccgggcc
    10201 gtctacctgg cggaagcagc catcggcgtg gcgctcgctt catggcagac accggaaggg
    10261 gaataaaccg aaaaccgcaa gcgaggcggg taactctaag aagacgcagg tcagcgagtc
    10321 atcgcaggcg cagtatggta acacgatgac tctgcctaag tccttcgccc acgctcaaga
    10381 cccgttgcat caacgcaagc cagtgtcgca gtcccagtgg acgttcgtca tcttaccctc
    10441 accaacgtca acctcgaacg gtccgtaaga accgatggaa accttccagt ccttgcgcga
    10501 aatggtgtag tgacctggct tcagcgaaac ccgaaactcg cctagatcat ttgtttggaa
    10561 cgcgccgatc acgcggcctc cctgctttac ctcgaaggcc atattggtgg cgggctttga
    10621 gtcggagatg ccttgcctgg tcggcccacc tcgcgcaggg ctgagggaga tggttccctc
    10681 cagtccggtt gagttatcag gagtggaggc cggctgcgtc tgtgcctgag cgaagccaag
    10741 aaacagacca gcgaagccaa gagatagaag caggctcgtg ttcatgaaag aagtttcggc
    10801 tgaaatgaag tgaagatcgc tcgacgcctg ggcgcggcag cacattatct tcgcacaacg
    10861 aggctcgaaa cgcgagcact gaggcgagca gcgcgggcgc atgagccgct cgcagcgcta
    10921 accgcgcaac aacgcggcta ttcccggatg ccccgcttca gctcccaaac tcgcaaggcc
    10981 gccatgatca atctccgcgt tcgcgtcatc gtcccgatgc ccggccggcg gggctcatcc
    11041 tctgccgccg cggtctccgg cggtggagcc tgtttgcgcc ctcgcttcga gaccgacccg
    11101 cgcgccgggc gggttgtttc tcgactcggg agaacgcctt caccggaagg gggtggattc
    11161 atgattgatc gtcaaaactg catctggcga tcctcaggcc agcgggtctt ttaatattta
    11221 tccgcacgcc ggattcctcg tcgcgcaagg catcttgctg gaggcttggt ttaagcgcgc
    11281 ctggcggcta ggaaatgggc tcggtaaact ccgccaactg gccgggacca gcgagcgcca
    11341 gcgcggcata aaattctgac cgcgttcttc cgctcccacc acgcttgaga tcggggaaga
    11401 ttctccccga ccgttcactc gcggaccctc tgcttcaact cccagacgcg cagcgccgcc
    11461 agcaccaggt tccgcgtccg ccttaacgtg cctaccgccg cgcgcctttc ctcgtcgtcc
    11521 gcggcgtggg ctcctctcaa cgggcgacgc ttcgactgac gcctcaactg gttcttcgct
    11581 ggtcggcgac caatcgttcc acttgtcttg agattcccaa atccgttgct attcggagtc
    11641 attgcggatg gttcggttag caacttgcaa gccagcattg cgcgacaacc atcagggaaa
    11701 ttttgcttcg cctcgcgcgt cgggctaaat gtctgcgcac gaggcgcctg aaatggagaa
    11761 aagaatcttc cgtgcgatgt tcgacttcac attccgccgc cacgcgctgg atcaatttca
    11821 gtgccaagtg ccaccaattg agaccaccag cggattatcc tttcgccgtc tctcggaacg
    11881 gccaacgcaa agcacctccc ttttcattgc cgtcgctggc tttcatcaat ccgaatcgtg
    11941 cggctccctg cacgggaaaa tcggctaagt tcatcgctcc acccgcctcg cgctccccgg
    12001 cactttgcgt gagcgctccg taaactgctc gtcgctatta tccgcgaccg tgttatagtc
    12061 gccgatggca aacccaacag gtaaagcatt ctcacgtcgc gctccggata ttattacgga
    12121 ggaactggtg gcggtgtttg ccagcaaggc atcgttcgag ttcaaaccgt tgttcgacgt
    12181 cgtcttcgcc aatttgcgcg cccgcaacgc ggcgagcggt ggggaggaaa tgctccggct
    12241 gcgctcttac gagaaactcc agagcctggt ggatcgcggc gcggtgaaga agacgattgc
    12301 gggcaccgtc aagaaataca agggagtttc cgctcccttg accgtcttga acactgagtt
    12361 gcaacagacg cgcctggctt ggagccagcg tgcggttggc tcggctgccg ccgcctgatt
    12421 gatccatggc aagacccaac tattctggcg agaagcggcg aaaagaactc gagaagcagc
    12481 ggaaaaaagc ggagaaaaag cggcgcaaac tcgagcacgc ctccgggcct ccgaaggatc
    12541 ctcaggtcga agaaagcgtt ccgccgtcgg taacagccgt ctccgacggc acggcaggac
    12601 cgctcgggta agacatttcc tcggaagtcg gcttttccaa caggaactcg cctgcggccg
    12661 aatggcccca gggccacatc accccgagca gcgaagaagg aatcccgcgg ctaatactct
    12721 ccaactcctc aatcgcgccg cgccgcggat aagaaaaacg ccttacggct tcttggcggc
    12781 tttctctccc ttggcgtcca cctcggttgc cgtcatcgta tacatgacgg ttaccttcga
    12841 acccactttc acgtcgccgt ttatcttggt actggcatcg cgcgcaactt cccagcgctc
    12901 tttgcctttt tgcacggcaa tcatcgtgtc attcatctcg agcaccggcc cggtcacctg
    12961 atacgatttc gtcgagccgg catcggtcgc gctcgcgaag gccagcactc ccgccgcaat
    13021 cattccgcat gtagtggtta acttcataga acgcgattga atcaggcggc gaaccactct
    13081 ggcaagtctc ttcttcgtgt ccggaaagtc gtcctgcttg atgccgagca atgcgacccg
    13141 ccacgtagtt cgttggtcgc atatgattcc cgtcgatccg gagcgttcgg agaacgctcc
    13201 atcggacgcc cgaaatctcg cagcgcagcc aattcgccgg ctgcggacct acaatttctt
    13261 cgcaagcatc gcgccggagc aaaaaagccc cgcaaatggc agcacggcaa accacaaaat
    13321 cgcggctccc agcatcagaa aaggttcgac gaaaaatgcc ttcggcccga tgcgtcgaga
    13381 gacgagcggg aatgttgacc ttccgggcgg acgcaatatc aatctcatac taaatggatc
    13441 gcacgggcgg gcgctttttc cagcagggat cgatcctgtt aacgtaacgt aatcaaacgg
    13501 taatcgttgc ctcgctcgat gataacccct tgaattttcc gaatgattac cctctgactc
    13561 gccgcgttgt aagtcatcgc gaaatagcac cgggttcacg gaacgggcgc atggaatagc
    13621 tggcggcagc ccactcgcct ggaaactctt cgcaatcgtg ctgtagatac ccagtatcgc
    13681 agcaagaccc cagatcgaag gggaacaagg agcacggtac ggtcgatcat ctaatgtctc
    13741 cgttcatctg cacgccgatc actcgttgcc gcaccgtttg gggagcggaa tccagagatc
    13801 ggaagcgttc cgcaagggcg gggcgagtgt tgaaacgggt atcaaatctg ctgctattag
    13861 agttgcagac acgagacgaa gaagaccgca gtgtgcttga acctttcttc gggttggtgt
    13921 cttttggttt gtcgtatggc cgctgaaaaa gaggaaccga agccgaggga aaaaaaggag
    13981 ccgaaacccg gaattcgcga tttgaagccg aagaaggatg taaaaggggg cacgcaaaaa
    14041 agccccggca gcgacaggcg gcctccaggc ggcaccggcg aaatcgactt catgaattgg
    14101 ggctaacgcc aggcgccgcc gccccttcga agacgcgaca taaagtcgcc cggcttgaat
    14161 cccgatggcg gtgcagactg tggcacgggt tgcagaaaag acgattgtcg atcatgcgct
    14221 ttgacgtcgc cgtcgtcggt ctcggaggga tgggcagcgc gctccttgcc cagtgtgctc
    14281 aacgtggcgc gtcggtgatc ggcctggagc agttcgcgcg cggacatgag ctggggtcgt
    14341 cgagcggcaa gagccggatg atccgcaaag catatttcga ggatcccgct tacgtgccct
    14401 tgctgcggcg cgcttatgat ttgtggcgcg agctggagcg cgctaccggc gcagaactct
    14461 tgcgtcgcac cggactcttg atggtgggcg aggcggattg cgagatcatc gccggtgccg
    14521 gccgggcggc acgcgagcat ggtctgcctc tcgagtcact caccgcgcgc gagatcaggg
    14581 agcgctatcc aatgctcacg gtactggacg gcgaagtcgg agtctacgaa tccgacggcg
    14641 gcgtcctcga cccggaacgg gccgttgagg ctcatctggt cgcggcggcg acggccgggg
    14701 cgcagatgcg ttttggggtc gctgtgcaga attgggaagc aacccgtgaa ggcttcgcca
    14761 ttcacctggc tgacggcacg agcgtcgcca gccgggccct catcttgtcg ttagggccat
    14821 ggttcaagga tgcgctcgaa agaatcggca tctcgatccg cgtgcagcgg aatgttcagg
    14881 cctggtttga gcccgccacc gaagcctacg cagcgcctca ttttcctcct tttctcctcg
    14941 accgaaaagg cctgcccgcg ccgctctatg gcttccctga tttcggcgat ggcgtgaagg
    15001 cggcgttcca ctcctacggc gacctgaccg acgccgcgaa cgttgttcgc gaagtggacc
    15061 tggcacgcga catcgcaccg atcgtccacg cgatggaaca atggatgccc ggcgccgcgc
    15121 taaagctgcg agcggccaag ccatgcctct acacactcac gccggattgc cattttgtgg
    15181 tcgaccgcca tcccgaacat tcgcggctga tcttgtgcgg cggattttcg gggcacggct
    15241 tcaagttcgc gccggtcatt ggggagatag gcgccgacct cgcgctgacg gaaagctcgc
    15301 ggcacaaaat cgattttctt tcgttgcgac gattcgcgcg gaaataaggc gtatcgttag
    15361 caaaaacgag gactacgcct ggagggcatc gcgcgggcgg cgcctgaagt ggacgccgcg
    15421 cgacaaaacc gcgcgcaaat tcgccgccgg ccgcacttcg cgctagaaag aaccatggca
    15481 atcaaagcgc agttcgcagc cgaacagtcg gccacggggg aattcgagcg gcaggacgat
    15541 gcatttcgcg actgggtttc cgccgacggc tcaacgcctt atcccgcggt ggccgggcgt
    15601 tatcaccttt acgtctcgct cgcctgccca tgggccagcc gcaccgtcat cgttaggctg
    15661 ctcgaaggac tggaggaagc catcggcctg acggtggtgg atccggtgcg cgacgagcga
    15721 ggctgggctt ttcgcgatgg gccgggctac agccgcgatc cggtgaacga tttctcgttt
    15781 cttcgggaag cctacgctgc gaccgacccg gcctgggatg gccgcgtaac cgtgcccgtt
    15841 ctctgggaca cggaaacgca ccgcatcgtg aacaactccg aggatgacat ctgccggatg
    15901 ttcaacggcg ccttccgcgc gctcgggcgg cgctccgtcg agcttttccc taacgacatc
    15961 gcgcaggaac aggccgagct ttccgccttc atttacgacc gggtgaacaa cggcgtttac
    16021 aaggccggct tcgccatccg gcagcgcgcc tacgaacatg cctgtcgtgc gctcttcgcc
    16081 gcccttgacc agctcgagat ccgcctaacg acaagccgtt atctattcgg ggatcggatc
    16141 gtcgaatccg attggcgcct cttctgcacc ctcgtccgct tcgatatcgt ttattacatt
    16201 cacttcaagt gcagcctccg gcgcattctc gattatccca acctgcagag ctatctgatg
    16261 gacctctttc aacagccggg catcgctccg accgtcaact tcgaacacat caagcatcat
    16321 tattacatga cgcacgatgc cattaacccg acccgcatcg tgccgatcgg tccgctgctc
    16381 gatctcatgc gtccgcacgg acgcgagaaa ttatcggcgc catgagcggt cgcagcggag
    16441 ccacccccgg aatttttcgc acggaagagc gcgcttctga cacgttaggc gaaatacctt
    16501 ctgcgtgatt gacctgcggc ccgcacttcg ctaaaacccg ggccatggcc gatatccaca
    16561 ttagtcgcag cggaaccact ctcggggttt ttcccgaaga agaagtcagg gaaggtctga
    16621 agacagcgcg ctttgcgccg accgatcttg gctggcgcga agggatgagc acgtggctgc
    16681 cgctcgcgca attcccggag ttcgccaccg cgggtgcccc gccagccgcc accggcgccc
    16741 tcccctcggc cagcgtaccc ggcgcgcccg gcgcacggac tggcctgccg tgggatcgcc
    16801 ggcgagagct tggcttggtc aacgccttta tcgagacgct caaactcgtc ctgttgaatc
    16861 ccaccgtggc cttctcggcc atgcaaaccg aaggcggact cgccgagcct ctcatctatg
    16921 cggtgatcgg cggcagcgcg ggattgatcg tttatttcct cttcagcatt ttcttcggtt
    16981 ccttcggcat aatgtctcat cggaatccgc tggccggcat catgggattc ggcatcggcg
    17041 ggattttctt tgtcatcttc gtgccggtcc tgcttgccat cggtctcttt atcggcggcg
    17101 ccattctcca tctctgcctc accttggtgg gcggagcgag acgctctttc gagacgactt
    17161 tccgtgtcct gtgcttcgcc gcggggtcga cctatccgct catgatcatc ccgctttgcg
    17221 gcggcctcat ctccggcatc tggtgcatcg tgttgcaatg catcgggctg gcccgcgccc
    17281 acgatacgac caccgccaaa gcagcgctgg cggtcttctt gcccttggtc gtctgctgcg
    17341 gcggcggcct gattcttggg ctcacgtttg gcacgctcgg cgccctcttc ggtcaccacc
    17401 attaatccaa cgagccgatg aaggcttacc gccgcccgct cgccccgcgg gagatcgatc
    17461 acgagctgct ctggctcgtc gtctccggtg caacatttgc cggtctcgcg ttctggctcg
    17521 ccgcgcggtt gcccacaccg cagtgcgcct ttcgctctct taccggattg ccctgcgtca
    17581 cttgcggcgc cacccgcgcg gccatccagt ttttccacgg ccatttcgcc gcctccttcc
    17641 tcttcaatcc gctcgccttc ctcacccttt gcagcttgct tgcctttgat ctctacgcgc
    17701 tgaccgtcct catcgctcgg gcgccacggg tccgcctcgg aaatttctcg cgcgcggaaa
    17761 aacgcctggc ccgctccgtg gcgatcatcc tcctcgccgg caattggatt tatttgctca
    17821 ccgcccaccc gttttaggac cagcggcgcg tggttgcgcc ggtgctgaaa caggaatccc
    17881 ttcgggtgaa aatccgctat tttcccgctc cggggtgaga cgcgagggac aagcgaatcc
    17941 tgcgctaata cgtttccacg ggggaacgtt tgccgtcttc agggctcggc actttggcgc
    18001 gcgcttcgcc cagaacggcg acacgcagat cccaaatctc cgcgttcaag gaggcgaagg
    18061 tcgactcgga aaattcatgc ggtggagcga ggttctcctt gaagcgcgcg atgttcgcga
    18121 gcgcgcggtc gaggtcggct cgggggagtt tttcaagata gccgtgcgct tcggcgatgg
    18181 cgggcacgcg atggcagatc atggccagat cgttcccagc gccaatcgcg cgccggatcg
    18241 tctcctcgaa gccgtactct ttgaggatcg cacccatgtc gaggtcgtcg gtcatgacga
    18301 gccccacgaa gccgagctcg tcgcgcaaca actcggtgac gatttttcgc gaaagcgagg
    18361 cggctgtctt ttccgcctcg agggtcgggt accacccatg gcagatcatc atgctatcga
    18421 cgcgatcgat cagcgcgcgg aagacggcca gctcgtttgc gtcgagttct gcccgcgtcc
    18481 gctcgatcac cgggagatcg tgatgcgcgt cgacggtggc cgcggagtag ccggggaaat
    18541 gttttccgca gctcgcgatt ccttgcctgc gcagggcgtc attaaaggca cccgccaacc
    18601 aaacggtctc agcgacgtcg cgaccgtagc aacgcccgcg gagcgaatta tccgcctcgt
    18661 catcaaacgc tatatcgagc accggacaga gatcgagatt gaagccaaac aagcggagca
    18721 ggcggccggt gatgtcgccg tgtcgcctaa cgagttcgag atcgcccttc tcgcgcagct
    18781 gttgcgcgtt cggcggctcc tgaccgatca ggcgcaggcg cgaaacgcgg ccgccttcct
    18841 ggtcgatcgt gatgatcggc tcgagcgcgc tcaggtcgcg caggtcgtcg atcaacttcc
    18901 gcagctgacc cgcgctctcg atgtttcgcc cgaagagaat aaaaccgccc ggctgcacct
    18961 tgcggaaaag ggcggccgtc ttgccatcga gttcagtccc gggcacgccg gtaaggatca
    19021 attgacccgt tgaagcgctc tccataacgt cggaccttag aatcgatgca cgacacgcga
    19081 aacaaaaaat ggttcgttag gctggccgcg ctcttgtctc gcgccgcgag cgcgcagggc
    19141 tcggtcgagc tcggaatcga cgtgttggcg agccacgatt attcgctcct cgccggcaaa
    19201 cgggtcggat taagtacgaa ccggacgggc gtcaactctg ccggcatcgc gagctggcgg
    19261 cctaacgagc aggagtttcg gacccgcgcg caagatctgc tctactgaag gcgcgcttga
    19321 aaaaaatcgg catttacggc ggcagtttcg atccgattca ccacggccat ctcatcctcg
    19381 cccgggaagc gctcgagaac ctcgacctcg cggaagtgat cttcgttccc gccgcgcaat
    19441 cgccgcacaa actcgcccga ccggcggccg gcgccgcggt gcgctgggaa atgctctccg
    19501 tcgcgatcgc gcacgagggc ggcttctcgg cttcccgatt ggaaattgat cgtcccccac
    19561 cctcctactc catcgtcacg gtggaacagt tgcgcgccaa gaaaacgaac gcggaatttt
    19621 tcttcctcgt cggggaggat aatctgccgc aattgccgac ctggcatcgc ttcaacgagt
    19681 tgcgccagtt ggtgcagttc gtcgtcctcg accggtcagg cgtggtcctc gacgacaact
    19741 atcctgtggt ccggcgcaag atcgatattt cggcgacgac aatcagaaat cgagttgcca
    19801 gcggccagtc gattcgttat ctcgtgccgg aagcggtgga gcaaatcatc cgccgggaga
    19861 atctttacca aggagccaat cgatcacacc tgaaaagcta gcgaaaacct gcgcagaact
    19921 cgccgcggat aagaaagcgg aaggcgtcgt ggtcctggat ctgcgcgaga tttcgacttt
    19981 cacggatttc ttcgtcatct gttcgggcac ctcggagccg catctaaagg cgattgccgg
    20041 cgagatcgag acgcggctac ggcaggatca taagattaaa ccggcagcgg tcgacggttt
    20101 cccggcgagc cagtggatcg tgctcgatta cctgcaggtg atcgtgcacg tcttccacca
    20161 ggaaaagcgc gatttctaca gcctggaaga cctctggagc gacgccccgc gcctaacgat
    20221 cgcgggcgtc gcttaaatga aataacttac ggcttctgcg gcgaggccgt agccgccggg
    20281 gaagcggcgg gcttcggcag tttgggcgga atcgcagcac ccggttggcc ggtcggccgg
    20341 ctcgagtcgt aggccgcggg cttgggtgtt ggggtagtcc ctgccggggt gattccaagc
    20401 aattccacgt cgaaaaggag ggtcgaattc ggcccgatgt cagagccggc gccgcgttcg
    20461 ccataggcca ggttaggagg aataaagagc tgatacttcg agccgacttt catcaactgg
    20521 agcgcctcag tccagcccgt gatgacgcga ttgaccggaa aagtcgccgg ctcgccgcgt
    20581 ttgtaggagc tgtcgaactc cgtgccgtcg atcgtcgtcc cgcgataatt gactttcacc
    20641 gcatcgaccg ccttcgggct cgggccgctg ccttccttga tgaccttgta ttgcaatcca
    20701 ctggcggtcg tcttcacacc ttccttcttc ttgttttcct caagaaactt ggtcccttcc
    20761 gccgcgttct tttccccggc tgccttcttc gtggcggcct gctttgccat catgtctttc
    20821 tggaaagtag ccatcgtcgc ctttacttcc tcgtccgtca ggagcggttt cttgccgctt
    20881 aatccatctt taatcccgtt gctcaggagg tcttggttga cgtcgatcaa ctggcgtttc
    20941 agagtggtgc caatgtcgag cccaatactg tagctgacct tgtctttctg atcttttagc
    21001 tgcgggtcgg ccgctggggc gagcggcgca agggcgaaaa cgacaacggc aatgagaggc
    21061 aattttttca tagatatttt ctgtaaacct ggatacgacc ggctcagttt tcggcccgcc
    21121 gcgctgaatt catccacggg gattgcgttc gcgtcaagcg cacgaacgct ggcgcgccgg
    21181 ccgggtccgt cgtcggccaa aacctgcgct ctggccgccg ggctctccag ccttcttttg
    21241 ctcgacgttt ccgcagcggt ttctctctcg catgaggacg ccgtgcggat cggccaccgg
    21301 atctggcaga atgaatgcgg agggacagtg gccggcctca cttcctggaa tgcgggcgaa
    21361 aactttgctt cgctcggaat cgggcacttc atttggtatc cggccgggaa gcgcggccca
    21421 ttcgaagaaa gtttccccga gttcgtccgc tacgctgcgc aacgcggcgc gaagttgccc
    21481 aagctgctgc tcgcgaacgg aggagacgtt tgcccatggc catcgcgcgc ggaattcctc
    21541 gccgcggaat cgggcgggaa gatggagacg ctgcgccaat ttctcgttga tacgattgat
    21601 ctgcaggccg acttcctggt caaccgcatg caggcagcct taccgaaaat gctcgccgcc
    21661 gcatcgccgg ctgaacgcgg gaaagtcgcg caacaattca accgcctggc gagcagcgcg
    21721 ccgggatgct acgcgctgat cgattacgtg aacttcaaag gcgagggcgt cttgctgagc
    21781 gagcgctacc aaggccgtgg ctggggactc ttgcaggtgc tcgaggaaat gtcgggctcc
    21841 ggcgatgcga cggcggccgt gaacgaattt gcggacgcgg cgacgcgcgt tttacgggtg
    21901 cgggtcgaaa atgcgccccg cgaacgaaac gaggcgcgct ggctgcccgg ctggttgaac
    21961 cggatcgaca gctaccggta ttaataccgt ggttaggcga agcccggttt gcgcgttagc
    22021 gcagagcgac aaagacgacc agcgcagccc cgaagggaga tcaccgcccg cgcccgcgat
    22081 ccacctctgc acgcgaccgc catggacccg ccaaggcgct ggaatcgcgc cggagcggca
    22141 aaccaaacct tgccgcgcga cgccgcattt caatttccgc gttgccacgg acctgctaat
    22201 tatccatttt gcattcgtta cagggttgaa aactcccgac ccactgattt ttgtgatcgc
    22261 ccgactttca agactttccg ttccgctgct cctcgccctg ctcgcgctgg ccaatgtaaa
    22321 cgcgcagctc tcgcgtccgt tcgcgcgtcc gcagccgcac gccacgccgc cgatgaagcg
    22381 ggcgagcgaa ctcatcaacc ggcaggagcc aatcaacgtt tcaccccgac tgcttgcgcg
    22441 cctgacacca gacaactccc acgtccgggt atcgatctcc aagcaacgcg cctacctgat
    22501 gatgggcgac gaaatcgtga tcgactcccc catttcatct ggcaaacgcg ctcggccgac
    22561 gccgcagggc agttttcacg tcctggaaaa ggacctgaat cacaaatcca ccatctacgg
    22621 cgatttcgtc gactcctccg gccgggtcgt ccgtcgcggg gtgagtagcc ggatcgactc
    22681 cgcgccgagc ggcacgcatt tcgtcggggc cgacatgaaa tggtttatgc gcctgacgga
    22741 cgaaggcgtc gggatgcacg ttggcttcct gccaggctat gcggcgtcgc acggctgcgt
    22801 ccgcatgcca tcatcggcgg ccgagatgtt ttacaaccac gtcaaggtcg gcacgccggt
    22861 cgtggtcggc gattaggctt ttgccaagcg ctcgcgcagg agattgagcg cggtctgagt
    22921 ggcgagttgc ttgaaggtct cgcgatcggt tgggaagaag agacgcctaa cgactgtttc
    22981 gccagcgccc gc
//
LOCUS       DATMNS010000288         2416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3821892, whole genome shotgun sequence.
ACCESSION   DATMNS010000288 DATMNS010000000
VERSION     DATMNS010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3821892"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VNW28_10100"
     CDS             complement(<1..207)
                     /locus_tag="VNW28_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10325.1"
                     /translation="MPQPAHKWSFESAAHLLNRAGFGGTTEEIEATHAAGLQAAVYNL
                     LHPPNEPGNEGTHPWTVPEDVQARM"
     gene            332..934
                     /locus_tag="VNW28_10105"
     CDS             332..934
                     /locus_tag="VNW28_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10326.1"
                     /translation="MRILARSALGIMGSIALLGVWVAGARPLSLLLDRLHTVRIHSQP
                     ITQLGVGEVSGGMLRINDLAMSTAMPNYSPFPLTMKVDPARQFVVQSGADAIALGRVE
                     DSSGEGRGTVVKPELDDQARFQIERSMLSWPTPFEINFMSGHSPSWKRHLYYRLLWDK
                     ADGGRLEMVWRYEQYYYDHWASGFMTREGTTGLIRVDIRP"
     gene            1071..1619
                     /locus_tag="VNW28_10110"
     CDS             1071..1619
                     /locus_tag="VNW28_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10327.1"
                     /translation="MSIILAAAAALCFWQAARHPLLLLVAPFFCFLRLWCNMLDGMVA
                     LAAGRASWRGEVLNDLPDRVSDVVIFVGAAHSGWMNPLLGYWAALLSLGTAYVGLLGQ
                     AVGGQREFSGLMSKPWRMVVVGLGAWLSYALFRWNDGDTHLGSLRALDWACLVVIAGC
                     LQTSVQRLGRILRALKAKSKDD"
     gene            1619..>2416
                     /locus_tag="VNW28_10115"
     CDS             1619..>2416
                     /locus_tag="VNW28_10115"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXA10328.1"
                     /translation="MSPSAALHNSVFRAYTGITLGVLVVAGIVLLFLQIVFRMELGSA
                     WKTYRSWLWLAPLAALVIFAGRVPFIIGVTVVSLLGCKEFAQVSGLARDRWMTGAAYA
                     AILFSAGGSGPLFALGSILLIPILRNRKASVVAAGGDPGRPETWPPGSPARITDPGSS
                     ELQRMSLGVVAFVYLGWMFGQLAFLANAGNAYGYLCYVIFATEVSDVAAFTFGKMFGR
                     HPLRSRISPRKTWEGALGALGVSIALPWLLRFSFPFFGTWQLILAGLI"
BASE COUNT          364 a          713 c          796 g          543 t
ORIGIN      
        1 catccgcgcc tgcacgtcct cggggacggt ccaagggtgg gttccctcgt tccccggttc
       61 gttaggggga tggagcaaat tgtaaacggc cgcctgcagg ccggccgcat gggtcgcttc
      121 gatctcctcc gtcgtcccgc cgaagccggc gcgattcaag aggtgcgccg cgctttcgaa
      181 actccacttg tgcgctggtt gtggcatcag catggtttcg acagatgacc ggcgggaaga
      241 gggtccggtt acaaaggtga gacactccgc cgtcgcggag attcgagatc ggcatggacg
      301 actcgcgccg tggcagatat gacttcaccg gatgcgaatc ttggccagga gtgcgcttgg
      361 gatcatgggc tcgattgcgt tgctcggcgt ctgggtggcg ggcgcgcgac cgctctccct
      421 cttgctcgat cgactgcata cggtgcgaat ccactcccag ccgatcaccc agctcggggt
      481 tggggaggtg agtggtggaa tgctgcggat caacgatctc gcgatgagca ccgccatgcc
      541 aaattacagc ccgttcccat tgacgatgaa ggtcgatccg gcgcggcaat tcgtcgtgca
      601 aagtggtgcg gacgcgatcg ccctcgggcg ggtcgaagat tcgtcaggcg aaggccgcgg
      661 gacggtggtc aaaccggagc tcgacgacca ggctcgattc cagatcgagc gcagcatgct
      721 gagctggccg acgccgttcg agatcaattt catgtcgggc cattcgcctt cctggaaacg
      781 gcatctctat taccggctcc tctgggacaa ggcggatggc ggccggctcg agatggtctg
      841 gcgctacgaa caatattatt acgaccattg ggcgagcggc ttcatgacgc gcgagggcac
      901 gaccgggctg atccgcgtgg acattcgccc gtgaattcgg caagcgtagg cgcatgccag
      961 gctatcaacc gacgtcacgc cgaccgatcg cggacgggtt tcgactgaca gcgcgcgggg
     1021 cgactcgctt ttgcgttcgc tttggtatcc atcccgacac gatttcgtat ttgtcgatca
     1081 ttttggcggc tgcggcggcg ctgtgtttct ggcaggcggc gcgccatcca ctgctcctgc
     1141 tggtcgcgcc gtttttttgt tttctgcgcc tgtggtgcaa catgctcgac gggatggtcg
     1201 cgctcgccgc gggccgggcg agctggcgcg gagaagtgct gaatgatctg cccgatcggg
     1261 tttccgacgt ggtgattttt gtcggtgcgg cgcacagcgg ctggatgaat ccgctcctcg
     1321 gttattgggc ggcgctgctg tcgttaggca cggcctatgt cggcctgctc ggccaggcgg
     1381 tcggcggaca gcgggaattt agcggcctca tgtcgaagcc gtggcggatg gtggtggtcg
     1441 gtctcggcgc gtggctgagc tacgcgcttt ttcggtggaa tgacggcgac acccatctcg
     1501 gcagcttgcg ggcgctcgat tgggcttgcc tcgtcgtgat cgccggctgt ctgcaaacga
     1561 gcgtgcaacg tctcggccgc atcctgcgcg cgctcaaagc gaagagtaaa gacgactgat
     1621 gtctccgtcc gcggcgctgc acaattcggt tttccgcgct tacacaggta tcacgctcgg
     1681 cgtgctcgta gtcgcgggaa tagtgctctt gtttttgcaa atcgttttcc ggatggaact
     1741 cggaagtgct tggaaaacct accgctcctg gctttggctg gcgccgctcg ccgccctcgt
     1801 gatttttgcc gggcgcgtgc cgttcatcat tggcgtaacg gtcgtttcac ttctgggttg
     1861 caaggaattc gcgcaagtct ccggtcttgc gcgcgaccgc tggatgactg gcgcagccta
     1921 tgccgccatt ctcttttccg ctggtggctc cgggccactt ttcgcgctgg ggtccattct
     1981 tctcattccc atcttgcgca atcgcaaggc gtccgttgtc gccgcgggcg gggaccccgg
     2041 ccggcctgaa acatggccac cgggcagccc agccaggatc accgaccccg gctccagcga
     2101 gctgcagcgg atgtcgttag gcgtggtcgc gttcgtctac ctcggctgga tgtttggcca
     2161 gctggcgttt ctcgccaacg ccgggaatgc ctatggttat ctttgctatg tgatcttcgc
     2221 gaccgaggtg agcgacgtcg cggccttcac tttcggaaaa atgttcggcc gtcatccgtt
     2281 gcgcagcagg atcagtccgc gtaagacgtg ggagggcgcg ctcggcgctc tcggggtgtc
     2341 gatcgcgttg ccgtggctgc tgcgcttttc ctttcccttc ttcggaacgt ggcaactgat
     2401 tctcgcgggg ttgatc
//
LOCUS       DATMNS010000289        11760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2104484, whole genome shotgun sequence.
ACCESSION   DATMNS010000289 DATMNS010000000
VERSION     DATMNS010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2104484"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="VNW28_10120"
     CDS             complement(<1..410)
                     /locus_tag="VNW28_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10329.1"
                     /translation="MMPSSPVNPATFRDRRNFALARWSELAAPLLKGAANVSCRGRPT
                     PSSSWRRGVLIGADHIGDVLYNTASLPALAEAFPECEWHFLATPPASEVLANNPFIKS
                     CVESLDALSRVDVAICYNSGGYWRDLFGAIRQRIP"
     gene            465..1997
                     /locus_tag="VNW28_10125"
     CDS             465..1997
                     /locus_tag="VNW28_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10330.1"
                     /translation="MPFGDRQIFGPRVGLGLMTGLGLVVCVAIGLTMGSNSLPIVIVI
                     ATIVLALIWVTRAKQRWWLLVPAAGTLGGYFWFGFKLYPHEVALAGSLIPLMITTAIR
                     RSDRIENRPPFPAVLYLLSLYLLAHWLGSNIYNKLDGGGGYGNVTRAYFNAYWVIIFL
                     IAFWRFGSTEYIPAALFLSYLAAAFRVIVGLLTYFTSAFAYIPVINYVLPGSTHSRGA
                     DLRASGLTLATLAACYFLVQKGFARKSFHFLIFLGSCVALLFGSGRTVLVLVCLIPIF
                     AALLYRRVVPLIFSLLAVTTVLVVLNAQPALLDHVSFPVQRSASILLFDQAQADKYGL
                     TESSDEWHANLRVLGFKKWTESWHNFLFGTGIRPYDNAISETIAGVTTNEDYLESSAK
                     VGAYESGWWTVIAVTGLVGLILYLIVLIYLLRRLLPILLREKVRDHAHAFAFMAVFGI
                     VIWLGLGWTNGGFPSTEIMFGFLALLAIEDGRSGQQPVATIEGHRGIALSPLRRVVPA
                     VR"
     gene            1994..2962
                     /locus_tag="VNW28_10130"
     CDS             1994..2962
                     /locus_tag="VNW28_10130"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXA10331.1"
                     /translation="MKPQETGRTVFFEPWSLGDVFIAAAALRELTIPAALACHSAWHP
                     LVRRALSATTELELIAIDLPYTTRKRANPFDASGAAAGNATSDIAEVLSIRGDFRDLS
                     AARKLFPGARIRMNGWLRFFGRKSAIVNFPFALGLLAVENRYRSWAKLVGIASTKIEA
                     TYRRLQLQAPGNRRVLIHVGAQWRSKQYPDVSGLRNALRGQGWEVTILASPRDLLPDD
                     LSENEISRVADEALIEELRAAAHVITNDSGPMHVAAFLGCRTTALVRTSPIDEWAPPA
                     TQIIRSPETPRGYRPNRQYMSDKVLPGWPSVAEITAAIRSAAATQT"
     gene            3173..4342
                     /locus_tag="VNW28_10135"
     CDS             3173..4342
                     /locus_tag="VNW28_10135"
                     /inference="COORDINATES: protein motif:HMM:NF015247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HXA10332.1"
                     /translation="MSRPHYVIVGAGLAGAVLARELVNGCDCRITICEGRDHIGGNCH
                     TERDPATRIMVHKYGPHIFNTDREGVWNYVNRFARFRPYVNRVKAKIDRGIFSFPINL
                     HTINQFFGKTLDPTEARLFIASLADRSIGEPANFEEQALKMLGGDLYEAFFRGYTRKQ
                     WGCEPRELPASILKRLPVRFNYNDSYYDKTYQGIPEEGYTELVRRLLDHEKITVLLNA
                     PFESTPLPKLDHLFYTGPIDAFYQFKLGRLGYRTVTFERIDAQVDYQGNAVINYPGMD
                     VPFTRIHEHKHFAPWEKHETTVAFREFSKETEPDDVPYYPKRLGDDLTLLQDYYTLAQ
                     AEKSTSFLGRLGTYRYLNMDEVIAESLDFAASSLACLSRGERPPVFPLRKGSPEA"
     gene            4339..5103
                     /locus_tag="VNW28_10140"
     CDS             4339..5103
                     /locus_tag="VNW28_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXA10333.1"
                     /translation="MSRFCFSLREIGESIDAELVVVMPIYNEAANIAPVISAWSATLR
                     QSGVSFQIVALDDGSRDETHRILLELETQNPELICVVSKPNAGHGSTCRIGYDIAINS
                     AAKWVLQIDSDGQCDPKYFVEFWAKREHADCVFGMRTSRDDGVPRILTSAICRLGSSL
                     ICGRDLKDANVPYRLMRRETVRRALEHIPPNFNIHNVALTYVLKKMPDVRWEYVAIHF
                     PDRTAGENSINLLKVMTWGAEMLLELLRIRVGSDKA"
     gene            5120..5626
                     /locus_tag="VNW28_10145"
     CDS             5120..5626
                     /locus_tag="VNW28_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXA10334.1"
                     /translation="MRPVQAWARNARVRFFLPHLHPGARILEIGAGDGWFKKAITASL
                     RVDYLTIDTHAPADVRGDVREWRRHGLKASSFDAIVAFEVVEHTPCFKECHELLKSDG
                     RLLVTTPMPQFDWLLNVFESLRLTQKRTSAHANLVSVKTVPGFVLERARYPFGLGQWA
                     ILRKVEPA"
     gene            5623..7101
                     /locus_tag="VNW28_10150"
     CDS             5623..7101
                     /locus_tag="VNW28_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10335.1"
                     /translation="MKLALPQRESERLWFFALAALAVSALAINQQSLWIDEGNTAAKA
                     LQPSFQAWWTSFLHERGSDLQMPGYMFFVWAWEKLAGASEVALRASNIPFFVGGVVAL
                     AWGQAKNRRAQAALVLLALTNAFLWYYLSEARPYLVLFGFAAVTAACLFRLRQDPEES
                     LQSGVWFRLFCAGTIGLCATSLIAVPWALGAIGAVFYWGGWRAVGRCALRCKAAAALS
                     MTALSGLGLYYLWTLQLGARASDVGRTGWSNIAYIFYELLGLAGLGPGRLALRTEGAG
                     EVLAFLPMMIAGTLAVLILLLTGFIALKGRINQRSVLFFAIAVALPFVLVLAAGASSH
                     MRLVGRHLTPLLPFLLAWMAIGLGRLLSARALRRKSLAVGLLAVLLLSALEIRLAPRH
                     RRDDYRSAAAIARAALDSGQRVCWLADVSTGLYYHVPFGSPRLATSLNLPGPNPPPDV
                     VILSKPDIYDASGAVRSYLAAEDFKVTRSLPAFQIWTRPPVR"
     gene            complement(7043..7774)
                     /locus_tag="VNW28_10155"
     CDS             complement(7043..7774)
                     /locus_tag="VNW28_10155"
                     /inference="COORDINATES: protein motif:HMM:NF023410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HXA10336.1"
                     /translation="MTPKRLIVLLLFLAAGFSAIFFLPTTAKTQPVGIRLELPQFVGK
                     WYGVDQKIGASELATLASDTEFARKLYTDAAGNAIFASIVLSGSDLDNSIHRPERCLP
                     AQGWTIADSRRFQIPLAGGQKLGVTRLHDVRQVPLSSGEVVTVYNLNYYWFVGYHHLT
                     ASHLEREMMDIQDRVFKGYNQRWAYITVAADVTQGLVRFGHSEAETDAMIQDFIRQVF
                     PEISKSPNEPAVASRSEKPAGSGSL"
     gene            complement(7764..8729)
                     /locus_tag="VNW28_10160"
     CDS             complement(7764..8729)
                     /locus_tag="VNW28_10160"
                     /inference="COORDINATES: protein motif:HMM:NF021254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase/archaeosortase family protein"
                     /protein_id="HXA10337.1"
                     /translation="MEQILQLRGILGSFLTWIRKRPGPAFLLFAVTATVVYFFGFLPL
                     YANQSQPIWEWAWLRFLPQYNQEHSKLIPFAVLFLVWYHRDKIRVARKEGSNLGLIFV
                     AFGVLCYLIAGRAFQPRVALFGFPFLVYGMVLYLWGRELARILLFPIALLFFMIPLGA
                     IEQTTFRLQFIIIGFVKTLSSLCGIPIYTVGTSIFPVHGNWGFEIAEGCSGIRSLIAM
                     IMLTAIYVHLCENKIWKKITVLLASVLFAIVGNAGRIFTIIVLGQLGFARFAGGIYHD
                     WSSQLIFFPIALLCMLGFSRLLNTDYRKWFGKKPVKEKEGVVYDS"
     gene            8919..9392
                     /locus_tag="VNW28_10165"
     CDS             8919..9392
                     /locus_tag="VNW28_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10338.1"
                     /translation="MKLRISGFIAGALLAAVTAQAQIGKDTRAADLIYGSPVAIGTDN
                     QGFVQRAYRKGGRDIIATVVDGVIKRIVFRKFAGAAFTYDELRTIARREGILVSNNNP
                     LPKEGQAWSIKYGADPIRAVYDPAANTFTITQDSYYKARDEKMRSHGPGKKEPGQ"
     gene            complement(9564..9989)
                     /gene="rplK"
                     /locus_tag="VNW28_10170"
     CDS             complement(9564..9989)
                     /gene="rplK"
                     /locus_tag="VNW28_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HXA10339.1"
                     /translation="MAKEIVAHIKLQIPAGQANPAPPVGTALGPRGVNIMAFCKEFNA
                     ATQKQAGDILPVVITVFKDKSFTFITKSPPAAILLKKAANIAAGSKEPNKTKVGKVTR
                     KQIMEIVKTKAKDLNSRNDEAGFKIIAGTARQMGLEVVD"
     gene            complement(10032..10601)
                     /gene="nusG"
                     /locus_tag="VNW28_10175"
     CDS             complement(10032..10601)
                     /gene="nusG"
                     /locus_tag="VNW28_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXA10340.1"
                     /translation="MAHVLAPKDQWYVVHVLSGQENKVRDNIEKRVKKEEMTDVVFEV
                     LVPTERVSEIKRGKKSETTRKFFPGYLIVNMHLLDEENQLIGRTWYFIRETPGVIGFA
                     GTKDRPIPMRQSEVDNMLAQMREREEKVKPKVSFEVGETVKVADGPFQNQSGIVEEID
                     PERGKLRVSVTIFGRATPVELEYWQVERG"
     gene            complement(10604..10819)
                     /gene="secE"
                     /locus_tag="VNW28_10180"
     CDS             complement(10604..10819)
                     /gene="secE"
                     /locus_tag="VNW28_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXA10341.1"
                     /translation="MLAKTKSFFSEVRLELQKASWPWDPKEKGMKRYKELTDSTLVVI
                     IAMLLLGGYVALFDFILVNVIHFLTRL"
     gene            complement(10900..10972)
                     /locus_tag="VNW28_10185"
     tRNA            complement(10900..10972)
                     /locus_tag="VNW28_10185"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10937..10939),aa:Trp,seq:cca)
     gene            complement(10990..>11760)
                     /locus_tag="VNW28_10190"
     CDS             complement(10990..>11760)
                     /locus_tag="VNW28_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003640798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HXA10342.1"
                     /translation="DMVDDPELLDLVEMEVRDLLTQYEYPGDKIPIVKGSALKALNGE
                     AEAEAQILALVAAVDDYIPVPERPVDQPFLMPVEDVFNIEGRGTVATGRVERGILKKM
                     EEVEIVGLTDTAKTTATDIEMFRKLLDEARAGDNVGVLLRGVKKEDVMRGQVIAKPGS
                     IKPHKKFKAEVYVLSKEEGGRHTPFFTNYRPQFYFRTTDVTGSVKLAEGVEMVMPGDN
                     VSVEVELGTPIAMEKTIRFAIREGGKTVGAGRVSEILD"
BASE COUNT         2289 a         3508 c         3422 g         2541 t
ORIGIN      
        1 ggaatccttt gacggatcgc tccgaagagg tcgcgccaat atccgccgct gttgtaacaa
       61 atcgccacgt caacccggct caatgcgtcc agcgattcga cgcaactctt gatgaagggg
      121 ttgttcgcca gaacttcgct cgccggaggt gtggccagaa aatgccactc gcactccggg
      181 aatgcctcgg ccagagctgg gagcgaggct gtgttgtaga ggacgtcgcc aatgtggtcc
      241 gcgccgatga gcactccgcg tcgccaggag gacgacggcg ttgggcgtcc gcggcatgaa
      301 acgttcgcgg cacctttcaa gagaggagcg gcaagctcgc tccaccgcgc cagggcgaag
      361 ttgcgcctgt cgcggaaagt cgcggggttg accggtgaag aaggcatcat cgctatgatc
      421 gcgaataaat tgccggacgc actccaacat tctactttgc gcgcatgccg ttcggggacc
      481 gccagatatt tggaccacgc gtcgggctag ggctcatgac cggactcggg ctggtggtct
      541 gtgtcgcgat cggcctgacg atgggatcca actcgctccc gattgtgatc gtgatcgcga
      601 ccatcgtcct ggccctcatc tgggtgacac gggcgaaaca acgctggtgg ttgctggttc
      661 cggccgcggg cacacttggc ggttattttt ggtttggatt caagctttat ccgcacgaag
      721 tggcgctggc gggcagcctg atccctctta tgatcacgac ggcgattcgc cgcagcgaca
      781 ggattgaaaa ccgcccgccg ttcccggcgg tcctctatct gctcagcttg tacctcctgg
      841 cgcactggct ggggagcaac atctacaaca aactcgacgg cggtgggggc tacggcaatg
      901 tcacccgcgc ctacttcaac gcttattggg tcatcatctt tctcatcgcc ttctggcggt
      961 ttgggtctac cgagtacatc ccggcggctc tttttctaag ctatctcgcg gcggccttcc
     1021 gggtcattgt tgggctgctg acctacttca caagtgcctt cgcctacatc ccggtcatca
     1081 actacgtcct tcccggctcc acccactccc gcggggccga tttgcgtgcc tcgggtttga
     1141 ctctcgccac cctcgccgcc tgttattttt tggtccaaaa gggctttgcc cggaaatcgt
     1201 ttcatttcct gattttcctg ggaagttgcg ttgccctgct tttcggctcc gggcgaaccg
     1261 ttctcgtcct cgtctgcctg atcccaattt ttgccgcatt gctctaccgc cgggtggtgc
     1321 ctcttatctt ttcgttgctt gctgtgacga cggttcttgt cgtcttaaac gcacaacccg
     1381 ctttgctcga tcacgtctcg tttcccgtgc aacggtccgc gtcgattctc ctcttcgacc
     1441 aggcgcaggc ggacaagtac gggttgacgg aatcgagtga cgagtggcac gctaacctgc
     1501 gggtgctcgg ctttaagaaa tggacggagt catggcacaa cttcctcttc ggaaccggca
     1561 ttcgcccgta cgataacgcg atcagtgaga ccatcgccgg cgtgacgacg aatgaagatt
     1621 acctcgaatc ctcggccaag gtcggcgctt atgaatccgg ctggtggacc gtgatcgcgg
     1681 tgactggcct cgtcggcctc attctctact tgatcgttct aatttatttg ttgcgccggc
     1741 ttctaccgat cctcctgcgc gaaaaagtgc gggatcatgc acatgctttc gctttcatgg
     1801 ccgtcttcgg gattgtcatc tggctcggcc tcggctggac caacggcggc ttccccagca
     1861 ccgaaatcat gtttggcttt ctcgcgctcc tcgcgattga ggatgggaga tcagggcagc
     1921 agccagtcgc cacgatcgaa ggtcatcgcg ggatcgctct ctcgccgctg cggcgggttg
     1981 tgcctgcggt gcgatgaaac cgcaagagac gggccggacc gttttcttcg agccgtggag
     2041 cctgggagac gtctttatcg cggcggccgc gctgcgggaa ttgacgattc ccgccgcgct
     2101 cgcatgccat tcggcgtggc atcctctggt gcgccgcgcc ttgtcggcaa cgacggaact
     2161 cgagctgatt gcgatcgact tgccctacac aacacgaaag cgcgccaatc cttttgatgc
     2221 cagcggggcc gcggccggaa atgcgaccag cgatatcgcc gaggtcctca gtattcgggg
     2281 cgacttccgc gacctgagcg ccgcccgcaa acttttccca ggggctcgca ttcgcatgaa
     2341 tgggtggctg cgttttttcg gacgcaagag tgcgatcgtg aatttcccgt tcgcgcttgg
     2401 tctgctggcg gttgaaaatc gctaccggtc atgggcgaaa ctcgtcggca tcgcctctac
     2461 caaaatcgaa gcgacctatc gtcgtctgca attgcaggcg ccaggcaacc ggcgggtgct
     2521 gattcacgtt ggggcgcaat ggcgctcgaa gcaatatccg gacgtatctg gcctgcgaaa
     2581 cgcattgcgc gggcaaggtt gggaggtaac catcctcgct tctcctcgtg atcttctccc
     2641 ggatgacctg tccgagaacg aaatatccag agtggccgac gaagcattga tcgaagagct
     2701 gcgcgccgcc gcgcacgtca tcaccaacga ttccgggccg atgcacgtcg ccgccttcct
     2761 cggttgccgc acgaccgccc tggtccgcac ctccccaatc gatgagtggg cgccaccggc
     2821 gacgcagatc atccgatcgc cggaaacgcc gcggggatat cgcccaaatc ggcaatacat
     2881 gtcggataaa gtcctgcccg gctggccttc ggtggcggaa atcaccgcgg ccatccgctc
     2941 cgccgccgcg acgcaaacgt aacgatttcg cctaacgaat cagatcctcc aacccttcgc
     3001 cgcggctggc ttggaacctc caacgtgtga tccactgcaa caagagcggg caaaacggga
     3061 gcaggccctc gagcgtgccg gcgggatttc gcggccggtt ctcagccggt gccaacgcgc
     3121 gtgcaatcgt tcgacgcttt ggacttttgt gctccattag gtcgcgattg gaatgtctcg
     3181 tcctcactat gtgattgttg gcgctggcct ggctggcgcg gtcttggcgc gagagctggt
     3241 caacggttgc gattgccgca tcacgatctg cgaaggacgc gaccacatcg gcggcaattg
     3301 ccatacggaa cgcgacccgg ccacccggat catggtgcac aaatacgggc cccatatttt
     3361 caacaccgat cgcgagggcg tctggaatta cgtcaaccgt ttcgcgcgtt ttcggcctta
     3421 cgtgaatcgg gtaaaggcga aaatcgaccg gggaattttt tcctttccga tcaatctcca
     3481 cacgatcaat cagtttttcg gcaagacttt agatcccacg gaagcccgtc tcttcatcgc
     3541 ttcactggct gataggagca tcggcgaacc ggctaacttc gaggagcagg ctctcaagat
     3601 gctgggcggc gatctttacg aggcgttctt ccgcggatac acccgcaaac agtggggctg
     3661 cgaaccgcgc gagttgcccg cctcgatcct gaagcgcctg ccggtgagat ttaattacaa
     3721 cgacagctac tatgacaaga cctaccaggg cattccggaa gagggctaca ccgaattggt
     3781 ccgccggcta ctcgatcacg aaaagattac cgtgcttctc aacgccccat tcgagtcgac
     3841 gccgctgccg aaacttgatc acctgtttta tacgggaccg atcgatgcct tctatcaatt
     3901 caagctcggt cgtctcggct accgcacggt gacgttcgaa cgcatcgatg cgcaggtcga
     3961 ttaccagggc aacgcggtca tcaactatcc gggaatggat gttccgttta cgcgcattca
     4021 cgaacacaag catttcgcgc cctgggaaaa acacgagacg accgtcgcat ttcgcgagtt
     4081 tagcaaagag accgagcccg atgatgttcc ctattacccc aaaagactcg gcgacgatct
     4141 gacgctgctg caagattatt acactctcgc gcaagccgaa aaatcaacct cgtttttggg
     4201 tcggctgggc acttatcgtt acctgaacat ggacgaggtt atcgccgagt cgctcgactt
     4261 tgcggcctcg tcgctggcct gcttgtcccg gggcgaacgg ccgccggtct tcccgctccg
     4321 gaaaggcagt ccggaagcat gagccgattc tgttttagcc tgcgcgagat tggcgaaagc
     4381 atcgacgcgg agctggtcgt cgtcatgccg atctacaacg aagccgccaa catcgccccg
     4441 gtcatctccg cctggtcggc aactctgcgg caatcggggg tatcatttca aatcgtggcg
     4501 ctggacgatg gttcgcgcga cgagacacac cggatcttgt tagaactcga aacgcaaaat
     4561 ccggagctca tttgcgtggt tagcaaaccg aacgctggac atgggagcac gtgtcgaatc
     4621 ggttacgata tcgcaatcaa ctcggcggcg aaatgggtgt tgcagatcga ttccgacggc
     4681 caatgtgatc caaaatactt cgtcgaattt tgggcgaagc gggaacacgc cgattgcgtg
     4741 ttcggaatgc ggaccagccg ggatgacggc gttcctcgca tcctgacgtc agcgatctgc
     4801 cgcctcggtt cttccctcat ctgcggccga gacctcaagg atgccaatgt cccgtatcgg
     4861 ctcatgaggc gggagactgt gcggagggcg ctggagcata tcccacccaa tttcaacatc
     4921 cataatgtcg cgctgaccta tgtcctcaag aaaatgccgg atgtgagatg ggaatatgtc
     4981 gccatccatt tcccggaccg gaccgccggc gaaaacagca tcaacctttt gaaggtgatg
     5041 acctggggag cggagatgct gcttgagttg ctgcgcatcc gagtgggttc cgacaaggct
     5101 tgagcgccgc agattcgtga tgcgacctgt ccaagcctgg gcgcgcaacg cacgggtccg
     5161 cttttttctc ccacatctcc atcccggcgc tcggattctc gagattggag cgggcgacgg
     5221 ttggttcaaa aaggcgatca ccgcttcgtt acgggttgat tacctcacga tcgacacgca
     5281 tgcgccggcg gacgtccgcg gcgatgtgcg ggaatggcgt cgccacggcc ttaaagcctc
     5341 ctccttcgat gcgatcgtgg cattcgaggt cgtcgaacac acaccttgtt ttaaggagtg
     5401 ccatgagctt ttgaaatccg atggccgcct cttggtcacc acgccgatgc cgcaattcga
     5461 ttggctgctc aacgtgtttg agagcctccg cttgacgcaa aagcggacca gcgcacatgc
     5521 caatctcgtc tcggtgaaaa cggtcccggg attcgttttg gaaagggcgc gatatccttt
     5581 tggtttgggt caatgggcga ttttgcggaa ggtcgagcca gcatgaaact ggcgctgccc
     5641 caacgtgagt cggaacggct ttggttcttc gccctcgccg cgctcgcggt cagcgcgctc
     5701 gcgatcaatc aacaaagtct gtggatcgat gaggggaata ccgccgcgaa agccctccag
     5761 ccttcgttcc aagcctggtg gacgagcttc ctccacgaga ggggatcgga ccttcaaatg
     5821 ccgggttaca tgtttttcgt ctgggcttgg gaaaaactcg ctggcgcttc cgaggtcgcg
     5881 ctccgcgctt cgaacattcc tttttttgtc ggcggtgtgg tcgcactcgc ctggggtcag
     5941 gcgaagaatc gccgcgcgca ggccgcgctc gtgctcctcg ccctaacgaa tgcgtttctt
     6001 tggtactacc tgagcgaagc gcgcccctac ctggttcttt tcggatttgc ggcggtgacg
     6061 gctgcctgcc tcttccgcct ccggcaggat ccggaggaat cgttgcaatc cggggtctgg
     6121 tttcgtctct tttgcgcagg tacgatcggc ctttgcgcca cgagcctgat tgccgttccc
     6181 tgggcgctgg gcgctatcgg cgccgttttt tactggggtg gatggcgcgc ggtcggccga
     6241 tgcgcgcttc gttgcaaggc agcggccgcc ctctcgatga cggcgctctc cggcctcggt
     6301 ctctattacc tctggacgtt gcaactcggc gcgcgcgcct ccgatgtggg ccgcaccggc
     6361 tggtccaaca ttgcctacat tttttacgaa ctgctcggat tggccggcct cggccccgga
     6421 agactcgctt tgcggacgga aggagcgggc gaagtcctgg cgtttctgcc gatgatgatc
     6481 gccgggacgc tggccgtcct cattttgctc ctgaccggtt tcattgccct aaagggccgc
     6541 atcaaccagc ggtccgtttt gtttttcgcg attgcggttg cccttccctt cgtcctggtg
     6601 ctcgcggcgg gcgcgtcctc gcacatgcgg ctggtcggac gccatctcac gccgctcctg
     6661 ccgttcctcc ttgcttggat ggcgatcggt ctcggccgtc tgctctcggc gcgggcactc
     6721 aggaggaaat cgctcgcggt cggactgctc gccgtcctcc tgctctccgc gttggaaatc
     6781 cgcctcgccc cccggcatcg ccgggacgat tatcgctcgg ccgccgccat cgcgcgcgcg
     6841 gcgcttgatt cagggcagcg ggtctgctgg ctggccgatg tgagcaccgg actttattat
     6901 cacgtgccgt ttggttcccc gcgacttgcc acgtcgctca atttgccggg gccaaatccg
     6961 ccgcccgacg tggtgattct ctccaaaccc gacatctacg atgcgtccgg ggcggtccgc
     7021 agctatctcg ccgcggagga tttcaaagtg acccgctccc tgccggcttt tcagatctgg
     7081 acgcgaccgc cggttcgtta ggcgatttcg aaatttccgg aaaaacctgg cgaatgaaat
     7141 cctgaatcat cgcgtcggtt tccgcttcgg aatggccaaa gcgcaccaat ccctgggtaa
     7201 catcggccgc caccgtgatg taggcccagc gctggttgta gcctttgaaa acgcgatcct
     7261 ggatgtccat catctcgcgc tcgagatgcg aggcggtcag gtgatgatag ccgacgaacc
     7321 aatagtaatt caggttgtaa accgtcacca cctctccact gctcaacggc acctgccgga
     7381 cgtcatggag acgggttacg ccgagctttt gcccgccggc cagcgggatt tgaaatcgcc
     7441 tggaatcagc gatcgtccag ccttgcgccg gaaggcagcg ttccgggcgg tgaatgctgt
     7501 tatcgaggtc ggagccggaa agaacaatcg aggcgaagat cgcgttcccg gccgcatcgg
     7561 tgtaaagctt gcgcgcaaac tcggtgtcgc tcgccagcgt cgcgagctcg gacgcgccga
     7621 tcttctggtc gacgccatac catttcccga cgaattgcgg cagctccaac ctgatcccca
     7681 ccggctgtgt tttcgcggtg gtcggcaaaa agaagatggc gctgaagccc gcggcgagaa
     7741 aaagcaaaag aacgatcagg cgcttaggag tcataaacga ccccttcctt ttccttgacc
     7801 ggctttttcc caaaccattt gcgataatcg gtgttcagga ggcggctgaa cccgagcatg
     7861 cagagcaggg caatcgggaa aaagatcaac tgactcgacc aatcatgata gatgccgccg
     7921 gcaaagcggg cgaaaccgag ctggccgagc acgatgatgg taaagatgcg tccggcattt
     7981 ccaacgatcg cgaagaggac ggacgcgagc aggacggtga ttttcttcca gatcttgttt
     8041 tcgcaaagat gaacgtaaat tgccgtgagc atgatcatcg cgatgagcga ccgaatgccg
     8101 ctgcaaccct cggcgatctc aaagccccag ttgccgtgca ccgggaagat ggaggtgccg
     8161 acggtgtaga tcgggatccc gcaaaggctg ctcaacgtct tgacgaaacc gataatgatg
     8221 aactgcaggc gaaaagtcgt ctgttcgatc gcgccgagcg ggatcatgaa aaagagaagc
     8281 gcgatcggga aaagaaggat gcgcgccagt tcccggcccc aaaggtaaag caccatcccg
     8341 taaaccagaa aaggaaaccc gaagagagcg acccgcggct gaaaagcacg cccggcaatg
     8401 aggtagcaga ggacgccgaa ggcgacgaag atcagcccga gattgctccc ctctttgcgc
     8461 gcgacgcgaa ttttgtcgcg gtgataccaa accagaaaga ggaccgcgaa agggatgagc
     8521 ttgctgtgct cctgattgta ttgcggaagg aaacgcaacc aggcccattc ccagatgggc
     8581 tgcgattggt tggcgtagag gggaaggaag ccgaagaaat aaacgaccgt cgccgtgacc
     8641 gcgaaaagca ggaaagccgg cccgggacgt ttcctgatcc aggtcagaaa cgagcccagg
     8701 attccacgga gttgtaagat ctgctccatg cggcggtccc gagtttaggg cgggcgtgag
     8761 cgcggcgcaa tcgagagacg tcgcctgacg aatcttcggc gacgaccgcc gcggggcaat
     8821 ttccgcgacc aggcagttgc gtgcatccgg tcggcgcgcg acgctgcaat ccgcccaaaa
     8881 ggcttcaaat cgaccaggaa acggtttaag aaagagccat gaaattacga atatccggct
     8941 tcatcgcggg ggcgctgctc gcagccgtga ctgcgcaggc gcagatcggg aaagacaccc
     9001 gtgccgccga cctcatttat ggaagtcccg tcgccattgg cacggacaac caggggttcg
     9061 tgcaacgcgc ttatcgcaaa gggggccggg acatcattgc cacggtggtg gacggcgtga
     9121 tcaaacgcat cgtttttcga aagttcgccg gggccgcatt cacctatgat gaactaagaa
     9181 ccatcgctcg gagggaggga atactcgtct cgaacaacaa cccgctgccc aaggagggac
     9241 aagcctggtc catcaaatac ggtgcagacc cgatccgggc ggtttacgac cccgcagcca
     9301 acaccttcac gatcacccaa gactcgtatt acaaggcgag agacgagaaa atgcgttccc
     9361 atgggcccgg gaaaaaggag ccgggacagt gatcaaaccc ccgcccggga tcgttgggcg
     9421 gcgataacgg aaagcggttc gtccagtccc ttccggggaa aaaaccgccg gcatctgcct
     9481 aacgaaaatc tagcccgccg aaaccagcag gctggcagcc tgcagctccc caattcgttt
     9541 aatccgcgta atccgcggtt gaattagtcg actacttcga ggcccatctg ccgggcggtg
     9601 ccggcgatga tcttgaagcc ggcttcatcg ttgcgcgaat tcagatcctt ggccttcgtt
     9661 ttcacgatct ccatgatctg tttgcgcgtc actttgccga ccttcgtctt gttcggctct
     9721 ttcgaaccgg ccgcgatatt ggccgccttc ttcaggagga tcgcggcggg cgggctcttc
     9781 gtgatgaagg taaacgactt gtccttaaag accgtgatca cgaccgggag aatatcgccc
     9841 gcctgctttt gcgtcgcggc gttaaattcc ttgcagaagg ccatgatgtt gacgccgcgc
     9901 ggtccgagcg cggtgccgac cggcggcgcc gggttggcct ggccggccgg gatctgcaat
     9961 ttaatgtgag caacaatttc tttagccata gggaaagttg agagtcttga gttgagagtt
    10021 gagagaggtg tttagccgcg ttcgacttgc caatattcga gttcgaccgg cgtcgcgcga
    10081 ccgaaaatcg tgaccgagac gcgtaacttt ccgcgctcgg gatcaatctc ttcgacaatc
    10141 ccactttggt tctggaacgg gccgtccgcc accttgacgg tttcgccgac ttcgaaacta
    10201 accttcggtt tgaccttctc ttcccgctcg cgcatttgcg ccagcatgtt gtcgacttcc
    10261 gattggcgca tcgggatcgg gcgatccttc gtccccgcaa aacctatcac gcccggggtt
    10321 tcgcgaatga aataccaggt ccggccgatg agctgatttt cctcatcgag caggtgcatg
    10381 ttgacgatca ggtagccggg gaaaaattta cgcgtcgtct cgctcttctt cccgcgcttg
    10441 atttccgaga cgcgctccgt cgggacgagc acctcgaaaa cgacgtcggt catttcctct
    10501 tttttgaccc gtttctcaat gttgtcgcgc accttgttct cctgcccgga gagcacgtgg
    10561 acgacgtacc attgatcttt gggagcgagg acgtgagcca tgattagagg cgggtcagga
    10621 aatggatcac gttgacgagg atgaaatcga agagcgcgac gtaaccgccg aggagcaaca
    10681 tcgcgatgat gaccaccagg gtggagtcgg tcagctcctt gtagcgcttc atccccttct
    10741 ctttcgggtc ccagggccag gaagcttttt gcagttccag gcggacttcg gagaagaaac
    10801 ttttggtttt agctaacatg cgggccgtga atcgttaaat cgtttcgttg ctctgggtcg
    10861 aatctcgaat ctcaattctc gtttcacgcg tccaaagacc aggtcaggag ggactcgaac
    10921 ccccaaccga cggttttgga gaccgctact ctaccaattg agctactgac ctaatactcg
    10981 tgttcaacac tagtccagaa tttcgctcac gcgacccgcg ccgacggttt tgccgccttc
    11041 gcgaatggcg aatcggatcg tcttttccat ggcgatcggc gtcccgagct ccacttcgac
    11101 cgaaacgttg tcccccggca tgaccatctc gacgccttcg gccagcttca cgctgcccgt
    11161 cacgtcggtc gtgcggaagt aaaattgcgg ccggtaattg gtgaagaacg gcgtgtgccg
    11221 gccaccttcc tctttgctca gcacataaac ctccgccttg aatttcttgt gcggcttgat
    11281 cgagcccggc ttggcgatga cctgcccgcg catgacgtct tcttttttca cgccacggag
    11341 gagcacaccg acattgtcgc cggcgcgcgc ttcgtcgagc agtttgcgga acatttcgat
    11401 atcggtcgcg gtcgttttcg ccgtatccgt gaggccgacg atttcgactt cttccatctt
    11461 cttgagaatg ccgcgctcga cgcgaccggt cgcgacggtg ccgcggcctt caatgttgaa
    11521 gacgtcttcc accggcatga ggaaaggttg atcgaccgga cgctctggca ccgggatgta
    11581 atcatcgacc gccgcgacga gcgcgagaat ctgcgcttcc gcctcggcct cgccgttcag
    11641 cgccttgagc gcgctgcctt tgacgatcgg aattttatcg cccggatatt cgtattgggt
    11701 gagcagatcg cgcacttcca tctcaacgag atcgagaagc tcgggatcat cgaccatgtc
//
LOCUS       DATMNS010000290        28682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4174395, whole genome shotgun sequence.
ACCESSION   DATMNS010000290 DATMNS010000000
VERSION     DATMNS010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 28682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28682
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4174395"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VNW28_10195"
     CDS             <1..331
                     /locus_tag="VNW28_10195"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXA10343.1"
                     /translation="LLVLLNPFAPHITSELWETLGKRFPGAPDEITAQPWPDWQPEFL
                     LEDEVEIALQVNGKVRDRVTVPIAAASAELEAIALANERVREFTAGKTIRKVVAIPKK
                     LVNVVAN"
     gene            344..802
                     /gene="mscL"
                     /locus_tag="VNW28_10200"
     CDS             344..802
                     /gene="mscL"
                     /locus_tag="VNW28_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXA10344.1"
                     /translation="MLKEFKEFAIKGNAIDLAVGVIIGAAFGAIVTSLVKDILMPPIG
                     LLTGGLDFSNKFLVLKAGANGAADFATPAQAAAAGAVTWNYGNFISLVINFIIVAFCI
                     FFVVKAMNKMKKPAPGAPAVSKDCPACAMTIPIKATRCPHCTSDLAGART"
     gene            888..1406
                     /locus_tag="VNW28_10205"
     CDS             888..1406
                     /locus_tag="VNW28_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10345.1"
                     /translation="MAKTTTLLRIALVSGFALLGASCACQSHKTASARSAPKRVCQVR
                     TTAYTRTEAGGGRNAIGSRLSGKNVMSAAADWSRWPLGTKFRIVDTDEVFQIDDYGSA
                     LIGTGTIDLYKTNRLAMNKWGVRSVDIDVIEWGSPEKSLEVLAPRAKNRRIRSMIFAL
                     SRKPNEQITRPF"
     gene            1453..2424
                     /gene="galE"
                     /locus_tag="VNW28_10210"
     CDS             1453..2424
                     /gene="galE"
                     /locus_tag="VNW28_10210"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HXA10346.1"
                     /translation="MKIFVVGGAGYIGSICVELLLDEGHEVIVFDNLTEGHRHAVDER
                     AAFIGGDLARGEEINRALQESRPEAVMHFAANALVGESMQNPSKYFRNNVAAGINLLD
                     AMLEAGVRRIVFSSTCATFGPPERLPIDETLPQRPINPYGESKLIFEKILRWYDEIHG
                     MKFVALRYFNAAGATEKFGEDHRTETHLIPNVLKVALGQKPQVEIYGTDYETPDGTCI
                     RDYIHILDLSRAHILALGVEKSAFYNLGTGGGASVREVVDVCRAVTGREIPVIEQPRR
                     PGDPPRLVAASAKIKRELGWQPQFENLPAIIESAWRWHQKFPAGYGD"
     gene            complement(2498..2911)
                     /locus_tag="VNW28_10215"
     CDS             complement(2498..2911)
                     /locus_tag="VNW28_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10347.1"
                     /translation="MAAGVAGAFALGTAFGQDSSTTTTTTAGGTTGISTTTTDTSALD
                     GTGTITTFSPDGNMMTVRTSTSTQPALFYYTPQTVVVDPTGATVQLSALRPDMPVSYT
                     YTKEGDRMVVTKVTLQKPITYYEKKETTTTTTTSP"
     gene            3083..4327
                     /gene="hisS"
                     /locus_tag="VNW28_10220"
     CDS             3083..4327
                     /gene="hisS"
                     /locus_tag="VNW28_10220"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXA10348.1"
                     /translation="MQPLPGFRDFLPDDCAVHNAIFARWRAVCRSYGFVEWDGPLLEP
                     TELYTKKSGPEIVAQLFNFTDKGGREVALRPELTPTLARVVAAHEREFRKPLKWFSIP
                     QCFRHEKQQRGRLREHFQLNCDIIGEASLAADTELIALSIDLLRAFGFTERDFVVRVS
                     DREFWTDLLRAQDVPEDRWSELLHVIDKSERESREKTAERLGPLAAPVLAVLDGGGRS
                     ERLDQLVRDLQTRGLAGFVKVDLAIVRGLAYYTGIVFELFDRSGKFRALAGGGRYDNL
                     IHQLSDGAVSLPAVGFALGDVVLGEMIRDVPAARARMADSIAGESALDIYLIIAKEER
                     RSDALRQLQQLREAGWRIDYPLTPAKVGKQFQTAEALGAKTTLLYGDEWPAVKMKTLA
                     TREEVLVPNEEVLARIKQALLS"
     gene            4430..6283
                     /gene="aspS"
                     /locus_tag="VNW28_10225"
     CDS             4430..6283
                     /gene="aspS"
                     /locus_tag="VNW28_10225"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HXA10349.1"
                     /translation="MTCEIQSFAQNDRLLAAMYRTHNCNALRAADIGEQVSLSGWVHS
                     RRDHGGVIFIDLRDREGLTQVVFRPEENAEVAQAAHHLHDEDVIQVDGRVAPRLPGTE
                     NPKLATGEIEVLPSRLTILNRADPLPFALDAEVSNEDLRLTYRYFDLRRPQLTRNLRL
                     RHRVAKATRDYLDAQGFLEIETPILSKSTPEGARDFLVPSRILPGKFYALPQAPQQYK
                     QLLMVGGVEKYFQIAKCFRDEDLRADRQPEFTQIDIEASFVRPDDIYALTEGMLAFIF
                     KAARGIDIPTPFPRMTYREAVGTFGSDKPDRRFGLQLVDLTEEFRASGFKLFRTIIEG
                     GGVVQAINAKGFAGITIGQVDELTAVAKNFGAKGLAFIKIENGEWKSPIVKFFSEAEK
                     SALRTKLALEEGDLIFFASDKWDIACEVLGRLRLRVAEIQGLTRSSEQLDFLWVTDFP
                     LLQFDAAENKWNAVHHPFTRPKAEDVPLLDEKRYGEMRAEAYDVVLNGVEVGGGSIRI
                     HEPELQAKMFAVLGVSEADQQIMFGHLLRAFRLGAPPHGGIALGLDRLVMLICGEQSI
                     REVMAFPKNNRGQDLMSQSPADVDLRQLRELRIKLAEAPRGPSETPPPRGR"
     gene            complement(6225..7826)
                     /locus_tag="VNW28_10230"
     CDS             complement(6225..7826)
                     /locus_tag="VNW28_10230"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HXA10350.1"
                     /translation="MDVRLLIDSGVLLLYFFIIIFIGLYMGRKENNLEDFALGGRRIP
                     WWAVLASIIAAETSAGTFFGTPGEGFALRNYTYLQLALGTILARILVSYIFIKPYYDY
                     KVFSIYEYLTARFGTRTKNAASAVFMITRLLASGARLYVGAIALALAYEMISGVRPSP
                     TQTLWIYVLSTVAIVILTAIYTTLGGIKAVIWTDVIQASIMFGSALVAVGLLYFSIPG
                     GWSEIIVRTGGFHFHDFITTGLDPAKHGWQKIVAMFGIEYTIFAGLFGATFITMGTHG
                     TDQDMVQRMLTAPDIRRSRRSLILSGLADIPIAFTFLTIGLLLWVYYQAHPDPTLPKT
                     PNETFCHFILYQMPVGLRGLLVAGIFATTMGSLSTALNALATSFTRDWYQPYINRSAT
                     EAQSLRAVRWATVGFAFLMIVVASATSYCVIVLPSVRIIPIALGIFGYTYGSLLGIFF
                     CGMLTRTRGRDALNPMAMIAGFLFVALISNLPNDIARIFGAKLYTPPAWLPIMEFPWW
                     ICFGTIVTFLVAVAFRSGRAALPPA"
     gene            8018..8884
                     /locus_tag="VNW28_10235"
     CDS             8018..8884
                     /locus_tag="VNW28_10235"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HXA10351.1"
                     /translation="MNIGELDIIAAPDFDLALTLDSGQTFHWEKTDAGFAGAIGARAV
                     YVEQQGERLFVNQGHAGLCFKYFALDHPLAEICASFPRDPAMQSAIAFCRGLRIIRQP
                     HWECLATFITSSMKQVAHIRQISQTLRRRFGEPCSLGELTVHSYPTPHRIAELREGDL
                     RDCALGYRAKNLLATARLISSGEEDIDQMATLPDDELRARLCALPGVGAKVANCVMLF
                     AYERLRAFPIDVWIERALRQHYFPGRRRITARQLRDFAAKHFGEHGGYAQQYLFHHAR
                     KTSPRRLSPPAS"
     gene            8972..10003
                     /locus_tag="VNW28_10240"
     CDS             8972..10003
                     /locus_tag="VNW28_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10352.1"
                     /translation="MEPNRDRRKIWWAVVASLVLHLLIGLSLAAFASRPQDLPLEEEP
                     PPQLTIMQMPEPTPPPLVPKNPPMTTVDPAHKSAVAPKEKTFEANENSIAAAEKAATE
                     TNSPLPGQDGKDRPFMNLEDHENSFASAGATPQPKPTPTAPPKKEPTPKATPAPKTPA
                     PAVETPAPNQLALLTQTPTPAASPEATEQNTEIPTPTATPTPTPEPSATPPPRASSYQ
                     DLQQKTIIRGGISNRGRSALNAVGTPLGRYKKLIEDAIGSRWYYYVNAKGDLISIGTT
                     AVTFEIMPDGKINNLRITENTTTEASASMALRSIQETKFPPIPDELLPTLPDGRFTME
                     ESFTIFANQ"
     gene            10000..10731
                     /locus_tag="VNW28_10245"
     CDS             10000..10731
                     /locus_tag="VNW28_10245"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXA10353.1"
                     /translation="MTTLFLAETTNPVQSVVDFLLRGGLFMWPLLICSIVSLTVIILR
                     TMALRRKYVLPLVIESEIERLSPGGSPERLSRIVREDDSSLARIARVALQHLRAPRSE
                     NIEAVETRARHEMVILERGLIVLEIITGIAPLLGLIGAVSGLVHVFSHLGLGTGTADT
                     KAIALGIAEALNATVFGLSIAVPTLIAFSYFSKKVEVMSVEMETLVVELISKCYYGRI
                     PEGMSMRAASSVPAAVMAHAPTPLT"
     gene            10754..11164
                     /locus_tag="VNW28_10250"
     CDS             10754..11164
                     /locus_tag="VNW28_10250"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXA10354.1"
                     /translation="MKFAVRKRRAPSIIIVSLVDILTILLIFFVVSTTFKKDQPEVEI
                     NLPDSKTSTSKPAEMEHAIVSVDAADALKLDGKAIAVDELQEAITNLPPARKSTLVLK
                     ADEKASFGIIIKVMDALKLAGVKNLPAFTQTPQK"
     gene            11161..11718
                     /gene="def"
                     /locus_tag="VNW28_10255"
     CDS             11161..11718
                     /gene="def"
                     /locus_tag="VNW28_10255"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HXA10355.1"
                     /translation="MKLPIFRYGEPVLRAKGKHVAAVDAHVRELAENMLETMRAANGI
                     GLAAQQVGEALQLTVIDVANAEDRPSTMKLNGAEVDPKSSMPLVLLNPELTLGEETDL
                     GVEGCLSFPEITADIWRAFSVALRAQTLEGAPVEIEASGLLARALQHEVDHLNGILFI
                     DRMSAATKVALRSRLKRLQKENAGR"
     gene            complement(11820..12338)
                     /locus_tag="VNW28_10260"
     CDS             complement(11820..12338)
                     /locus_tag="VNW28_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10356.1"
                     /translation="MRFLPAALVALLLVPAVSAVADEIPPPFGFRWSDPAARVEKVLK
                     GAKAKIGSRDSHDAQETWTVEGLIHPGLKRTLFTFKGGLLTQVELQYEYPGWTLEHYN
                     SRMGEVRRYFDAKYGTGRLISRARDNDTDVIQTLVGYQWVVGKSALELFYFSAQKEPN
                     VYRNITVTYKAL"
     gene            complement(12345..13109)
                     /locus_tag="VNW28_10265"
     CDS             complement(12345..13109)
                     /locus_tag="VNW28_10265"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HXA10357.1"
                     /translation="MLSIPFQVFSLFTSCSHPVSVLPACGFRLEIGAPAHKKKSELYF
                     ACRLRKTLTQNGLWRGPVFLSCGIFRRIGNLLGKVWRRACSDFMGRFSRLASRCENVR
                     QAIDLPEIRGPKQRMRQSGRNNCGRLDAATVLAICFAVFFAACSTTPAPSPTVTRVVL
                     VWLRHPGDPAARAQLIRVAQSLQMIPGVLRVETGRPVPGLGPDADRSFDLGVLITFRD
                     RAALQRYEKDPHHLEAIRRYLRPLVRRYEVFNLGER"
     gene            13351..14706
                     /gene="dnaA"
                     /locus_tag="VNW28_10270"
     CDS             13351..14706
                     /gene="dnaA"
                     /locus_tag="VNW28_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HXA10358.1"
                     /translation="MDEKCTQLWTKLSAALKPEVSADTFKRWFSAVKLTSATAEEVTL
                     LVPNNIYQFWIESNHMPALQAAIASVVGGPRTVKFVCAVDTSAGSDAADPEPAVSSRA
                     QAAAGRANKTPGLNPRNTFESFVVGPNNEIAHAASLAVAQSPAKTYNPLFIYGGVGLG
                     KTHLMQAIGQYIGARGKSTRVMYLSSELFINEFIDAIQHNNLVKFRKRYRQADLLLID
                     DIHFLGGKERSQEEFFHTFNTLFDGHKQIVLSSDRPASEIANLEHRLVSRFEWGLTAE
                     LQPPDVETRMAILRKKARTLKIRLRDEIFDFLATRIKTNVRRLEGALMRVASFASLSG
                     KELTHEVVEHLLKDILQEEARRAVTIDQIQRRVAEHFDVRLADMTSKRRPANIAFPRQ
                     IAMYLARELTKASLNEIGEAFGGRDHGTVLHACKLVKKRMKEQDKIRQTISFIDSALQ
                     R"
     gene            14778..15875
                     /gene="dnaN"
                     /locus_tag="VNW28_10275"
     CDS             14778..15875
                     /gene="dnaN"
                     /locus_tag="VNW28_10275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HXA10359.1"
                     /translation="MKFSVTKEKLLECLQQVQNVVSTRTTLPILSNVLLQANGKEVRL
                     TTTDLDVGVRGSFEAEVEKEGATTLPARRLFTIIRELPSSEIQFDIDGKNAASIRSGQ
                     SFFKILGLPEEEFPPLPKFDDSKVVTIRQKDLRDGLRKTSYAISTDETRYVLNGVLFS
                     FKDNKLTLVATDGRRLAMLDIDLEFPRSHETDIIVPTKAVTELQRLLTDEGDVRVSVG
                     TGQIAFDLNNTLLVSKLIEGNYPNYKQVIPGEMKERVTLERETFLNSLRRVSLLASDK
                     SNSIKLNFTKNNIDITANTPEVGEAKESLPVAYKGREFSIAFNPEFLMAPLRNLAEDE
                     IFLDLIDEMSPGVIKIQSPFLYVLMPMRISS"
     gene            complement(16063..16953)
                     /locus_tag="VNW28_10280"
     CDS             complement(16063..16953)
                     /locus_tag="VNW28_10280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXA10360.1"
                     /translation="MGRILVIRGGAIGDFILTLPALKLLRHGFPSAYLEVLGYKHIAV
                     LATMSGYANATRSIESASLASFFSRDGDLGPDLVDYFGSFQQVISYLFDPDRIFADNL
                     KRAGVRHLLVGSPKVTDQEHAARQLARPLESLGLYLDDPAAIILPPEGRRRDPSLIAI
                     HPGSGSESKTWPLERWVEIARSLLLPNDKTRLLLIGGEADHGRTPQLRAALPDERVEI
                     AQELTLADLAARLQECGLFLGHDSGISHLAAGVGTPCLLLFGPTDPAIWAPANAQVRV
                     LRAPGLTMDGISVEQVLAHL"
     gene            complement(16961..17506)
                     /locus_tag="VNW28_10285"
     CDS             complement(16961..17506)
                     /locus_tag="VNW28_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10361.1"
                     /translation="MIYHFSLYAVGLIAGALLALLSLAAIFLPARNYLPQFPRSRSAG
                     AALLTVDLIWSFWLLATMEMGEFSNFRRPLLIILPVGFVLVLKFVDEFLAVRALGILC
                     LLAAEPLLDAAFLRPEMSRLLVTVLAYLMIVAGLFWVTMPYLLRDQIHWSTKTAERWR
                     LVSGLGFALGAVIVACAVFVY"
     gene            complement(17503..18924)
                     /gene="miaB"
                     /locus_tag="VNW28_10290"
     CDS             complement(17503..18924)
                     /gene="miaB"
                     /locus_tag="VNW28_10290"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXA10362.1"
                     /translation="MPKFFIKTYGCQMNERDSEQVARSLLDRGYEAAASEAEADVVLL
                     NTCSVRDMAEQKALGKMGMLGRMAKDRPGAVFGLLGCMAQARGAELLKLIPHLDLVVG
                     TQKFHRVADYVDELMERKRAVRAMDDPRFSIVDTAEETGSQETIRDHTLRAHQASAFV
                     SIMQGCNMHCTFCIVPRTRGAERSRTIAEIVREVRRLIARGVKEVTLLGQIVNLYGRH
                     EFPRIDGKSPFVQLLEAVHEVDGLERLRFTSPHPIGFRADLIAALRDLPKLCEHVHLP
                     LQSGSDRILKAMHRTYTADKYLRLVDQIRAMRSDVALTTDVIVGFPGESEQDYEKTRA
                     LVERVQFDNAFVFRYSQRSETPAAEMGGQIDERVKEARNQDLLTIVDAAAKRAGERLV
                     GRRVQILCEGPSKTNRARLMGRARTNKIVVFAGSEADIGQLVDLQITHSTGFSLSGTK
                     MDRAGSCHLPPMEELLPRAAVAS"
     gene            19113..19982
                     /gene="mtnP"
                     /locus_tag="VNW28_10295"
     CDS             19113..19982
                     /gene="mtnP"
                     /locus_tag="VNW28_10295"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HXA10363.1"
                     /translation="MKSENAAIGIIGGSGLYQIEGFSNPREHQLETPFGTPSDTIIGG
                     EIGGRRVYFLPRHGRGHRLLPHELNHRANIYALRALNVRWIICVTAVGSLQEKYAPRD
                     VLLPSQFYDRTSQRADHTFFGDGVAAHITFAEPICTPLRNLLAEVAGSLGVRVHNGGT
                     YVNMDGPAFSTRAESDLNRRHGFDVIGMTNLPEAKLAREAEIALASMAMITDYDCWKI
                     DEEPVSAQTVVGHLLANAETAKRILAVVIPRVPTAPDWPEHRALDSALVTDQKLWPPA
                     TREKLRAILGRFG"
     gene            20132..20380
                     /locus_tag="VNW28_10300"
     CDS             20132..20380
                     /locus_tag="VNW28_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10364.1"
                     /translation="MKFKIALSLIAVAMLPACSTTKTASNTGDAKSSRYANNSVAEAV
                     PPAEGPTADIPSEGPHDVIANPAYMPSPLLRTSAAGGP"
     gene            complement(20423..21610)
                     /gene="bioF"
                     /locus_tag="VNW28_10305"
     CDS             complement(20423..21610)
                     /gene="bioF"
                     /locus_tag="VNW28_10305"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HXA10365.1"
                     /translation="MSDLFREQLEALRAQSLHRKLREIGTAQGPEVQIVGRHLVNFSS
                     NDYLGLAADPILRRAASAAIEEFGVGAGASRLISGTQSPHVALETALAKWKRAPAALC
                     FGSGYAAAVGTLPALASKRDVVILDKLCHASLIDGARLSGATIRIFPHNHLGKLESHL
                     EWARREHPQARVVIVTESVFSMDGDRAPLRALVELKNRFHACLLLDEAHAIGVVGPHG
                     RGLAAELGLEAQIDVQLGTLSKSLGVSGGYICGSRSLVDWLINRARSFIFSTAPPPAL
                     AAAATAAVHFLASEEGEARRHTLWRRIVQLRKMLPEVREEFGQPGAAIIPWMVGDEQR
                     ALDLAQALQKEGFLVPAIRYPTVAKGAARLRLTVSASHSEAQITSLGEAIGRLAEQTK
                     TAL"
     gene            complement(21631..22539)
                     /locus_tag="VNW28_10310"
     CDS             complement(21631..22539)
                     /locus_tag="VNW28_10310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HXA10366.1"
                     /translation="MIRRATGCLTFPLTCALALLCVAATQPNSLPVKRAQAVTDDEDS
                     SGGYQTHELLKGLNGPPRTRAAAVLVQDARSGEILYEKNADAPRAAASTQKLLTALIV
                     AEDGFLDQNVTVQLIDTQAEPVRLNIKPGESYSRIELLRALLVKSPNDVARCLARDNA
                     GSIELFAEKMNAKARALGATHSNFVNPNGLPIPDQYSTARDLSLIARAAYANPTIRSI
                     VCLPQLAFRYANGRVRELKNTNKVLKQVPYCNGMKTGYTEAAGHCLIASGKIPGRDII
                     VVVLGDSSAGIWRDASALLYWGLQRM"
     gene            complement(22580..22966)
                     /locus_tag="VNW28_10315"
     CDS             complement(22580..22966)
                     /locus_tag="VNW28_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXA10367.1"
                     /translation="MIKKLLHTRYRVKDLEKTVSFYRDVLGLEEVRRHTSGRGSQLVF
                     LKAPESEEEIELCKFDESGPVVVGPDLTHLAFEVDNIEEFAKQTAAKGYPLSDGPHPN
                     GRGGAIAFIDAPEGYEVELIQRGAGR"
     gene            23030..24529
                     /gene="xylB"
                     /locus_tag="VNW28_10320"
     CDS             23030..24529
                     /gene="xylB"
                     /locus_tag="VNW28_10320"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HXA10368.1"
                     /translation="MLSLGIDSGTKSTKSLVLDLTSGEVLALAQRSYGTIAGLPPGHV
                     EQDPKIWIEAAEATVAECLEKVGPRRAEIRSVGVSGQQHGLVPLDETNEPLRPAKLWC
                     DVSTESQCEQFNKEFGGGAGLIERLGNPMLPGYTAPKLLWLKENEPANFRRLTTVLLP
                     HDYLNLWLTGERQMEYGDASGTGLMDVRTRTWCKPILKFIDPKLESTLPPLRSSKRPA
                     GLLRSVLREKWGLADDALVSAGSGDNMMGAIGTGNIRAGVVTISLGTSGTICAFAEEP
                     VVDLKGEVAAFCDGTDHWLPLTCTMNVAVATEQMRALFGWDIPTMEQRVAAVPAGAGG
                     VLFLPYLQGERTPNLPRSSAVFHGLTTANMTPDFMARAVVEGVTLGLAYGLRRFQDLG
                     IGPTEIRLTGGGSKSAVWRQIAADILGFPTVALRVTEGAALGAAIQAAWTYSEVKGQP
                     LSLEELVDDLVKVDKKTRAEPRQEAQMFYHELLRRRIDLTKKLKTSGYL"
     gene            24706..25275
                     /locus_tag="VNW28_10325"
     CDS             24706..25275
                     /locus_tag="VNW28_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HXA10369.1"
                     /translation="MKLVIAATGASGTLYLQRLLEQIDTRANEVHLVLSGHAKQVAAT
                     ELERLVVPDGVRQHGENDLHVPFVSGSARFDAMVIVPCSMATLGRVACGSGDSALLRA
                     ADVFLKERRKLIVVPRETPWSLIHARNVVTLLEAGAIVLPAIPSFYSRPQSVNDLVDT
                     VVWRILDQIGLPNPRAYRWKDESAPSSEH"
     gene            25272..25916
                     /locus_tag="VNW28_10330"
     CDS             25272..25916
                     /locus_tag="VNW28_10330"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXA10370.1"
                     /translation="MKIEGWRARWLIAFGYRLLQIWARTLRFTIEDHAGVVGAPPNER
                     YIGALWHNRLLLLPFVLKRFLPERRGAALISASRDGALLADLVERFDFEVVRGSTSRQ
                     GASAIRQLAEIIARGKDVVITPDGPRGPAYELGQGIVYLAQQSGAAVVPINLEYSGSW
                     RVKSWDRFILPRPFSRVRVIFGAPHRVAQTSTEADFERERTRLQEAMIQLVEMR"
     gene            25913..26809
                     /locus_tag="VNW28_10335"
     CDS             25913..26809
                     /locus_tag="VNW28_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HXA10371.1"
                     /translation="MKLIDGRTIAEKVYAELRGEIAALKARGVTPGLAVVLVGDDPAS
                     RAYVRSKDKMCRELGLHSVKLELPAETTQSDLLARVGDLNRDPAIHGILVQSPPPRQI
                     DEAAIIRALDPAKDVDGFHPMNIAKLALGDATGFVPCTPLGCLRLLTESGVTISGAHV
                     VVLGRSMIVGKPLALLLMQKGTDATVTVVHSRTRNLAQITRTADVVVAAIGRAGFVRA
                     EHVREGAVVIDVGINRVEDAAAPRGYRLVGDVAFDKVAPKTAAITPVPGGVGPMTIAM
                     LMANTVKAAQLLSSADPAFASR"
     gene            complement(26757..27800)
                     /locus_tag="VNW28_10340"
     CDS             complement(26757..27800)
                     /locus_tag="VNW28_10340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXA10372.1"
                     /translation="MWDVAIVGSGPAGASCAAFCAQAGLRTLVLERAHFPREKVCGDC
                     LNPACWPVLDRLGVSVEVRRAPHGQLSMVEFIGLDGRSVTAPLPAGKDAEIAIKRSIL
                     DQILLRRARSLGAEVREGETLLSVTRGEHWQITTEQSVERAPVLVAADGRNSTVARLS
                     NLMPRKGRDRVALQTHVPLPLDFGDRVVLQLLAEGYSGQAPVGDGLLNLCLVSRPRDL
                     PAIRHWAERQFSIPVDHAWRTIAPLARAALPASQPGLFLVGDAARVVEPFTGEGIYYA
                     LRSGELAAAAIGAKDHRRYAAAHRRLYAGRLWINLLSRSAVLHPRLTTGLLRYLPNEK
                     RMLGRLTTKVVRL"
     gene            27784..>28682
                     /locus_tag="VNW28_10345"
     CDS             27784..>28682
                     /locus_tag="VNW28_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXA10373.1"
                     /translation="MATSHIFEYSSTTVPLSKEKPDLNKKVHEVPHKPGVYLMRDRFG
                     RVIYVGKARDLRKRVGSYFLPSKMAVADLKTRALLEAVWDFEIHTVASEPESILLEGK
                     LIKEYRPRYNISFRDDKRFLVVKVDPTEEWPRFRLARFKKDDGARYFGPYAHAGALRQ
                     TLNFMRKKFGVLTFGRGSPTERELKSSTYQVPTRLSEINAETYRDRVAQAAEFLEGHS
                     REMMNAVEEEMQKAAAKQDFEKAAELRNMLDDLRRTTRPMRRFTRHSLPSTIDPQADV
                     RALADALRLPNLPAVMECFDISN"
BASE COUNT         5793 a         8960 c         8685 g         5244 t
ORIGIN      
        1 actgctcgtg ctccttaacc ctttcgcccc gcacattacc tcggagcttt gggaaacgtt
       61 aggcaagcga ttcccaggcg cgcccgacga gatcacggcg cagccctggc ccgactggca
      121 gcccgagttc ctgctcgagg acgaggtgga aatcgccctc caggtcaatg gcaaggtgcg
      181 tgatcgcgtg acggtcccga tcgccgccgc cagcgccgaa ctcgaagcca tcgccctggc
      241 caatgagcgc gtccgggagt tcaccgccgg aaagacgatt cgcaaggtcg tggcgatccc
      301 gaaaaagctg gtcaacgtcg tggccaacta ataaatagga gccatgctta aggaattcaa
      361 agagttcgca atcaaaggca acgccatcga cctcgctgtc ggtgtgatta ttggcgcggc
      421 gttcggcgcg atcgttacct cgttggttaa ggatatcctg atgccgccaa tcgggctgtt
      481 gaccggcggc ctggacttct ccaataagtt cctggtgctg aaagccgggg ccaatggggc
      541 agccgacttc gccaccccgg cgcaagccgc cgcggccgga gcggtgacgt ggaattacgg
      601 aaatttcatc agcctggtga tcaattttat catcgtggcg ttctgcatct tcttcgtcgt
      661 caaagcgatg aacaaaatga aaaagccggc accgggtgcg ccggccgttt ccaaggactg
      721 ccccgcttgc gcgatgacga tcccgatcaa ggcaacccgc tgcccgcatt gcacgagcga
      781 cctggccgga gcacgcacct aggctgcgac tcgaaagcga ccggcgtgct tgcggttgag
      841 tgaaaattac tcttgccaac tccggcggca gccgctaagc aggcggcttg gccaaaacga
      901 cgacgctctt gagaattgcc ctggtgagcg gctttgcgct cctgggtgca agttgcgcct
      961 gccagtcgca taaaaccgcc agcgcccgct ccgcgcccaa gcgtgtctgc caggtgcgca
     1021 ccaccgctta cacccgcacg gaggctggcg gcggtcgcaa tgccatcggt tcccgcctct
     1081 ccggcaaaaa cgtgatgagc gccgcggccg actggtcgcg ctggccgctc ggaaccaagt
     1141 tccggatcgt ggatacagac gaagtcttcc agatcgacga ctacggcagc gcgctcatcg
     1201 gcaccgggac gatcgatctt tacaagacca accgcctcgc catgaacaag tggggcgtcc
     1261 gctccgtgga catcgatgtg atcgagtggg gttcgcccga gaagagtctc gaagtgctcg
     1321 cgccgcgcgc gaaaaatcga cgcatccgct cgatgatctt tgccctttcg agaaagccga
     1381 acgagcagat cacccggcct ttctaagcgg actttctttt gacagcggcg aggcccgtcg
     1441 caccttccgc ggatgaaaat ctttgtcgtt ggcggcgcgg gctacatcgg gagcatctgc
     1501 gttgagcttc tgctcgatga aggccacgag gtcatcgtct tcgacaatct gaccgagggc
     1561 catcggcacg cggtcgacga acgagcggct ttcatcggtg gcgacctcgc acgaggggag
     1621 gaaatcaacc gagcgttgca ggagagcagg ccggaagccg tgatgcactt cgccgccaat
     1681 gcgctcgttg gcgagtcgat gcaaaatccc tcaaaatatt tccggaataa cgtcgccgct
     1741 gggatcaacc tcctcgacgc gatgcttgaa gccggcgtgc gccggatcgt tttctcctcg
     1801 acctgcgcca ccttcggtcc gcccgagcgc ctcccgatcg acgagaccct gccgcagcgg
     1861 ccgatcaatc cctacggcga atcaaaactg atcttcgaga aaattctgcg ctggtacgat
     1921 gaaatccacg gaatgaaatt cgttgccttg cgttacttca acgcggccgg cgcgacagag
     1981 aaattcggcg aagaccaccg gacggaaacg cacctcattc ccaacgtttt gaaagtcgcg
     2041 ctcgggcaaa aaccgcaggt cgagatttac gggaccgatt atgaaacacc ggacgggacc
     2101 tgcatccgcg attatatcca catcctcgat ctctcacgcg cgcacatcct cgcgctggga
     2161 gtggagaaga gcgcgttcta caatctcggg actggcggcg gcgcgagcgt gcgcgaggtg
     2221 gtcgatgtct gccgcgcggt caccggcagg gaaattccgg tgatcgaaca gccgcgccgg
     2281 cccggcgacc cgccacgcct ggttgcggcc tcggcaaaga tcaaacgcga gctcgggtgg
     2341 cagccgcagt ttgagaatct gcccgccatc atcgaaagcg cgtggcgctg gcaccaaaag
     2401 ttcccggccg gctacggcga ttgattcggc agaaaagaaa cgcccgtgga cccacggtcc
     2461 acgggcggtt cagaatcagt cacgaacaat cgactcgtta ggggctggtc gtcgtcgtgg
     2521 tcgtggtttc cttcttttcg taataggtga ttggcttttg cagcgtcact ttggttacga
     2581 ccatgcgatc gccttccttc gtgtaggtgt agctcaccgg catgtccggc cggagcgcgg
     2641 agagttgcac agtggcgccc gttgggtcga cgacgaccgt ctgcggcgtg taataaaaca
     2701 gcgccggctg cgtgctggtg gacgtgcgga cggtcatcat attgccgtcg ggcgagaagg
     2761 tggtgatcgt gcccgtgccg tcgagcgcgc tcgtgtcggt ggtggtcgtg ctaatccccg
     2821 tcgtcccccc tgccgtggtg gtcgtcgtgg tgcttgaatc ctgaccgaaa gcggtgccga
     2881 gcgcgaacgc gccagcaact ccggcagcca acaattttgt gaatctgttc tgtttcatgt
     2941 ttgtcctttg gtttggttga acggatgatc tccgttgtgt ttttttccga ctcgtcccag
     3001 ccggcgttta ttcaccccca tttcgtaacg cgaccgatgg gcggttgcat ggctggataa
     3061 cgccccgatt tatcattcac cgatgcaacc acttcccggt tttcgcgatt tcttgccgga
     3121 cgattgtgcc gtccacaacg ccatcttcgc gcgctggcgc gctgtctgcc gcagctacgg
     3181 ctttgtcgaa tgggacggcc cactcctcga gccaaccgag ctttacacaa agaagagcgg
     3241 acccgaaatc gtggcgcagc tttttaattt caccgacaag ggtggccgcg aagtcgccct
     3301 gcggccggag cttactccga ccctcgcgcg agtcgtcgca gcacacgagc gcgagttccg
     3361 caaaccgctc aagtggttct ccatcccgca atgttttcgc catgaaaagc agcagcgcgg
     3421 ccggttgcgc gaacatttcc agctcaactg cgacatcatc ggcgaggcgt cgctcgccgc
     3481 cgacaccgaa ttgattgcat tgagcatcga ccttctccgc gccttcggtt tcaccgaaag
     3541 ggacttcgtc gtcagggtga gcgaccggga attttggacg gatctgctgc gtgcgcaaga
     3601 cgtccccgag gatcgctgga gcgaattgct ccacgtgatc gacaagagcg agcgcgagtc
     3661 gcgggaaaaa acggccgaac ggctcggccc gctggccgcg ccggtgctgg cagtgctcga
     3721 cggcggtggc cgcagcgaac ggctcgatca actcgtgaga gatttgcaga cgcgcgggct
     3781 ggccggcttc gtgaaagtcg atctcgcgat cgtgcgcggc ctggcctatt acaccgggat
     3841 cgttttcgaa ctcttcgatc ggtccggaaa attccgcgcg ctcgccggag gcggccgtta
     3901 cgacaatctg atccatcaac tgagcgacgg cgcggtttcg ctgccagctg ttggcttcgc
     3961 gctcggcgat gtcgtcctcg gggaaatgat ccgggacgtg ccggcggcgc gcgcgcggat
     4021 ggccgactcc atcgcgggcg agagcgcgct cgatatttac cttatcatcg ccaaggaaga
     4081 gcgacgctcc gacgcgctcc ggcagctcca gcaactgcgc gaggccggct ggcgaatcga
     4141 ttacccgctg accccagcga aggtcggcaa acaattccag actgccgaag cgctcggtgc
     4201 gaagaccacg cttctttacg gagacgaatg gcccgcggtg aaaatgaaaa cgctcgcgac
     4261 gcgcgaggaa gttctggttc caaacgagga ggtgctcgcg cgcatcaaac aggctctgtt
     4321 atcctgagcg gagcgcagcg aagtcgaagg atcccgtggc acaacctgca gccttcctgg
     4381 tcttcgacgc ttatcgtgca ttccccggga tccctcggct tcgctcggga tgacttgcga
     4441 gattcaatcc ttcgcacaga atgacaggct tctcgccgcc atgtatcgca cccataactg
     4501 caacgccctc cgtgccgccg acatcggcga gcaagtctcc ctctccggct gggtgcactc
     4561 ccggcgcgat cacggcggcg tcattttcat cgacctgcgc gaccgcgaag gcttgaccca
     4621 ggtggtcttt cggccggaag aaaatgccga ggtcgcacag gcagcgcatc atctgcacga
     4681 cgaagacgtg attcaggtcg atggccgcgt cgcgccccgc ctgcccggga cggagaatcc
     4741 aaaactggcc accggggaga tcgaagtgct gccttcgcgc ctaacgattc tcaaccgcgc
     4801 cgatcccctc ccttttgcgc tcgacgccga ggtcagcaat gaagatctgc gcctcactta
     4861 tcgatatttc gacctgcgcc gtccgcagtt gacgcgcaac ctgcgcctgc gtcatcgcgt
     4921 cgcgaaagcg acccgcgatt atctcgacgc gcagggcttc ctcgaaatcg agacgccgat
     4981 tctctcgaaa agcacgccgg aaggcgcacg cgatttcctc gttccaagcc gaattttgcc
     5041 gggaaaattc tacgcgcttc cccaggcacc gcagcaatac aagcaactcc tcatggtcgg
     5101 cggggtggag aaatatttcc aaatcgcgaa gtgctttcgc gacgaggacc tgcgggccga
     5161 tcgccagccg gaattcaccc agatcgatat cgaagcctcg ttcgttaggc cggacgacat
     5221 ctacgcactg accgagggga tgctcgcttt cattttcaag gccgcgcgcg ggatcgacat
     5281 cccgacgcct tttccgcgga tgacctatcg cgaagcggtc ggcaccttcg ggagcgacaa
     5341 acccgaccgg cggttcggcc tgcagttggt cgatcttacc gaggagtttc gcgccagcgg
     5401 tttcaaactt ttccgcacga tcatcgaggg cggcggagtc gtgcaagcga tcaacgccaa
     5461 gggctttgcc ggcatcacca tcggccaggt cgacgagctg acggcggtcg cgaagaattt
     5521 cggcgccaag ggtctcgctt tcatcaagat cgaaaacggc gaatggaaat cgccgatcgt
     5581 gaaattcttt tccgaagcgg aaaagagcgc gctccggacc aaactagcgc tcgaggaagg
     5641 cgatctcatt ttcttcgcct ccgacaagtg ggacatcgcc tgcgaggtcc ttggccggct
     5701 tcgcctgcgg gtcgcggaga ttcagggcct gacgagatcc tccgagcaac tcgattttct
     5761 ttgggtgacc gattttccgt tgctgcaatt cgacgccgcg gagaacaaat ggaacgcggt
     5821 ccatcacccg ttcacgcgac cgaaggcgga agacgtgccg ttgctcgacg agaagcgcta
     5881 cggtgagatg cgcgccgaag cctatgacgt cgtgctcaac ggcgtggaag ttggcggggg
     5941 cagcatccga attcacgagc cggaattgca ggcgaaaatg ttcgccgttc tcggggtgag
     6001 cgaggcggac cagcaaatca tgttcggcca tcttctgcgc gctttccgct tgggcgcgcc
     6061 gccccacggc gggatcgcgc tcggcctcga ccgtctcgtt atgctcatct gcggcgaaca
     6121 atcgatccgc gaggtgatgg cttttccgaa aaacaatcgc ggccaggacc tgatgtcgca
     6181 gtcgccggcc gacgttgacc tgcgccagtt gcgcgagctg cgaatcaagc tggcggaagc
     6241 gccgcgcggc ccgagcgaaa cgccaccgcc acgaggaagg taacgatcgt gccaaagcag
     6301 atccaccagg ggaattccat gatcgggagc caggccggag gcgtgtaaag cttcgcgccg
     6361 aagatgcgcg cgatgtcgtt agggaggttg ctgatcagcg cgacgaaaag aaaaccggcg
     6421 atcatcgcca tcgggtttag ggcgtcgcgg ccgcgggtgc gggtgagcat gccgcagaag
     6481 aaaatcccga gaagcgaacc gtaggtgtag ccgaaaatgc caagcgcgat cgggatgatg
     6541 cgcacgcttg gcagcacgat cacgcaatag gaggtggcgg aggcgaccac gatcatgaga
     6601 aaagcaaaac caaccgtcgc ccagcggacg gcgcgcaggc tttgcgcctc ggtcgcggag
     6661 cgattgatat acggctgata ccagtcgcgc gtgaagctgg tggcgagcgc attcaaggcg
     6721 gtgctgagcg agcccatcgt ggtcgcgaaa atgccggcca caagcaaccc gcggagtccg
     6781 accggcatct ggtagagaat gaaatgacag aaggtctcgt taggcgtctt cggcagggtc
     6841 ggatcgggat gcgcctggta ataaacccag aggagcaaac cgatcgtgag aaaagtaaag
     6901 gcgatgggga tgtcggccag cccggagaga ataagcgagc gccggctccg ccgaatgtcc
     6961 ggcgcggtca gcatccgttg caccatatcc tggtcggtcc cgtgcgtgcc catggtgatg
     7021 aaggtcgcgc cgaaaaggcc ggcaaaaatc gtgtactcga tcccgaacat cgcgacgatc
     7081 ttttgccagc cgtgtttcgc cgggtcgagc ccggtggtga tgaaatcgtg gaaatgaaaa
     7141 ccgccggtgc gcacgataat ttcgctccaa ccgcccggaa tagaaaagta gagcagcccg
     7201 accgcgacga gcgcgctgcc aaacatgatg gaagcctgga tgacgtcggt ccagatgaca
     7261 gctttgattc cgcctaacgt tgtgtaaatg gcggtgagaa tgacgatcgc aaccgtggaa
     7321 aggacgtaga tccagagcgt ctgcgtcggg ctcggccgca ctccgctgat catttcgtag
     7381 gcgagcgcga gggcaatggc gccgacgtag aggcgggccc cggaggcgag cagacgcgtg
     7441 atcatgaata cggcggaggc ggcgtttttc gtccgcgtcc cgaaacgagc ggtcaggtat
     7501 tcgtaaatcg agaagacttt gtagtcgtag tagggcttga tgaaaatgta gctgacaagg
     7561 atgcgggcga ggattgtgcc aagcgcgagc tggaggtagg tgtagttgcg gagagcaaaa
     7621 ccttcgccgg gcgtgccgaa aaaggtgccg gcgctggttt cggcggcgat gatggaggcg
     7681 agcaccgccc accacgggat acggcggccg ccgagggcga aatcctcgag gttgttctcc
     7741 ttccggccca tatacaggcc gatgaagatg atgatgaaaa aatagaggag cagaactccg
     7801 gaatcgatga ggaggcggac gtccaccgcc tgttagctgc cagaattcag cgggagcgca
     7861 aaggattttc caaagcttga atgagcgagg ccggatggtc attctgaggg ccacgcgcac
     7921 cgtgtctgat ccccttcgcc gaaacctcac gaacacgaat cactgatgtg cctatttcct
     7981 ttccgctcgc caggacagca gggcgttctc gcctgagatg aacatcggcg aattagacat
     8041 catcgcggcg cccgatttcg atctcgcgct cacgctggat tcgggtcaga cgttccattg
     8101 ggagaagacg gatgccggat ttgccggggc gatcggggcg cgcgcggttt acgtggaaca
     8161 gcaaggcgag cggcttttcg taaaccaggg gcatgccggg ctgtgcttca aatacttcgc
     8221 gctcgaccat ccgctggccg agatttgcgc atctttcccc cgcgatcccg cgatgcagtc
     8281 cgccatcgcc ttttgtcggg ggctccgcat catccggcag ccgcactggg aatgcctcgc
     8341 caccttcatc acctcctcga tgaaacaggt cgcccacatc cggcaaattt cgcaaaccct
     8401 gcggcgtcgc ttcggcgagc cgtgttcgtt aggggagctg accgtccact cctacccgac
     8461 accgcaccgg atcgcggagt tgcgggaggg cgatttgcgg gactgcgcac tcggttatcg
     8521 ggcgaagaat ttactcgcga cggcgcgact gatcagctcc ggcgaggagg acatcgatca
     8581 aatggcaacg cttccggacg acgaattgcg ggcgcggctt tgcgcgctgc cgggcgtggg
     8641 tgcgaaggtg gcgaattgcg tcatgctctt tgcttacgag cggttgcgcg cttttccgat
     8701 cgacgtctgg atcgagcgcg cgctgcggca acactatttc ccgggccggc gcaggataac
     8761 ggcgcggcag ttgcgcgatt ttgcggcaaa gcatttcggc gagcatggcg gttacgccca
     8821 gcagtatctt ttccatcacg cgcgcaagac gagcccgcgc cggttgtcac cgcccgcgag
     8881 ctgagataat tttccgcctg aagaaactcg ccggccagcc attgcagcgt tctcgttagc
     8941 cggcgctatg tctcggaacg gcgcaggtat catggaacca aatcgagatc ggcgaaaaat
     9001 ctggtgggcc gttgttgcct ctctcgttct ccatcttctc atcggcctct cgctcgcggc
     9061 gttcgccagc cgcccgcagg atctcccgct ggaggaggag ccgccgccgc agttgaccat
     9121 catgcaaatg cccgagccga cgccaccgcc gctcgttccg aaaaatccac ccatgaccac
     9181 ggtcgacccc gcgcacaaat ccgcggtcgc acccaaggaa aagacgttcg aggcaaacga
     9241 gaactcgatc gcggccgcgg aaaaagccgc gacggaaacc aatagcccgc tgccgggcca
     9301 ggacggaaaa gaccggcctt tcatgaacct ggaggatcat gaaaactcct ttgccagtgc
     9361 cggcgcgacg ccgcaaccga agccgactcc gaccgccccg ccgaaaaaag agccgacgcc
     9421 gaaagcgacg cctgctccca aaacgccggc ccccgcggtc gagacgccgg cgccgaatca
     9481 gctcgcgctc ctcacccaaa ccccgacgcc ggctgcctct cccgaggcga cggaacagaa
     9541 caccgaaatc cccacgccaa ccgccacgcc gactcccacg cccgagccca gcgcgacgcc
     9601 gcccccacgg gcctcgagtt atcaagacct ccagcaaaaa acgatcatcc gtggcgggat
     9661 cagcaatcgc gggcgttcgg ccctcaacgc cgtcggcacg ccgctcggac gttacaagaa
     9721 gctgatcgag gatgcgatcg ggtcacgctg gtattattac gttaacgcga aaggtgatct
     9781 gatcagtatt ggcacgaccg cggtgacttt cgaaatcatg ccggatggta agattaataa
     9841 tttgcgcatc acggagaaca cgacaaccga ggccagcgcc agcatggcgc tgcgctcgat
     9901 tcaagagacg aaatttcctc cgatccccga cgaactgctc ccgactcttc cggatggccg
     9961 ctttacaatg gaggaatcct tcacgatttt cgccaaccaa tgaccacgct ttttctcgcc
    10021 gaaaccacca accctgtcca atccgtggtc gactttctcc tccggggcgg ccttttcatg
    10081 tggcccctgc tcatctgctc gatcgtgtcc ttgacggtga tcatcctgcg gaccatggcc
    10141 ttgcgccgga aatacgtcct gcccctggtg atcgaaagcg agattgagcg cctctcgccg
    10201 ggcggaagcc ccgagcgtct ctcccggatc gtgcgggagg atgattcctc gcttgcccgc
    10261 atcgcgcggg tcgcgctgca acatctgcgc gcgccgcgct cggaaaatat cgaagccgtc
    10321 gagacgcgcg cccgccatga gatggtcatc ctggagcgcg ggctgatcgt gctcgaaatc
    10381 atcaccggca tcgcgccgtt gttaggcttg atcggtgccg tctcgggcct ggtgcacgtt
    10441 ttctctcatc tcggcctggg cacgggcacg gccgacacca aagcgatcgc tctcggcatc
    10501 gccgaggcgc tcaacgcgac ggttttcgga ttatcgatcg ccgtgccgac cctcatcgcg
    10561 ttcagctatt tttcgaaaaa ggtcgaagtg atgtcggtcg aaatggaaac gctcgtcgtg
    10621 gaattgatca gcaagtgcta ctacgggcgc attcccgagg gaatgtcgat gcgcgccgct
    10681 tcctccgtgc cggcggccgt gatggcgcac gcgccgaccc ccttgacctg acaaaccggc
    10741 cggatcgctg cccatgaagt tcgccgtccg aaaacgccgc gcaccgtcga ttattatcgt
    10801 ctcgctggtc gatatcctca ccatcctgct catttttttc gtcgtctcga ccaccttcaa
    10861 aaaagatcag ccagaagtgg agatcaatct gcccgattcg aaaacctcga cctcgaaacc
    10921 ggcggagatg gaacacgcga tcgtgagtgt cgacgcggca gacgccctca agctcgatgg
    10981 caaagcgatt gccgtcgatg agttgcagga ggcgatcacc aatctgcctc ccgcgcgcaa
    11041 gagcactctc gtcttgaagg cggacgagaa agcgtcgttt ggcatcatca tcaaggtgat
    11101 ggatgcgctg aagctcgccg gggtgaaaaa tctgccggcc ttcacccaga caccccagaa
    11161 atgaagttgc cgatttttcg ttatggcgag cccgtcttgc gcgccaaagg gaaacacgtc
    11221 gcggccgtcg atgcgcatgt ccgcgagctg gccgagaaca tgctcgagac gatgcgcgcc
    11281 gcgaacggga tcgggctcgc cgcgcaacaa gtcggcgaag cgctccaact gacggtcatc
    11341 gatgtcgcga acgcggagga ccggccgagc acgatgaaat tgaacggcgc cgaggtggac
    11401 ccgaagtcat cgatgccgct ggtcctgctc aatccggagc tgaccctggg cgaggagacc
    11461 gacctcggcg tggaaggatg cctgagcttt cccgaaatca cggcggatat ttggcgcgct
    11521 ttttccgtgg ctttgcgcgc gcagactctg gaaggagcgc ccgtggaaat cgaagcgagc
    11581 gggttgctcg cgcgcgcgct gcaacacgaa gttgatcacc ttaatggtat ccttttcatc
    11641 gaccggatga gcgccgcgac gaaggtggcg ttgcgcagcc ggttgaaacg gttgcagaag
    11701 gaaaacgcgg gtcgttaggc aggcagacgc ctccctccgg gagacgcgat gatgaattgc
    11761 ttgcgcgagc catccaccgg ccgacggagc cgcgcctcgc gttgcgcaga gcgaagcgtc
    11821 tagagcgcct tataggtcac cgtgatgtta cggtagacgt tcggttcttt ctgcgcggag
    11881 aagtaaaaca actcgagggc tgatttcccg accacccact gataaccaac aagcgtctga
    11941 atgacgtcgg tgtcgttgtc tcgcgcgcgt gaaatcaaac gcccggtgcc atacttcgcg
    12001 tcaaagtaac ggcggacttc gcccatccgg ctgttgtaat gttccagggt ccagcccggg
    12061 tactcgtact gcaactcaac ctgcgtcagc agcccgccct tgaaggtaaa aagcgtccgc
    12121 ttcaagcccg gatgaatcaa cccttccacc gtccaggttt cctgcgcatc gtgggaatcg
    12181 cggctgccga ttttcgcctt cgcgcctttc aggacctttt cgacgcgagc ggccggatcg
    12241 ctccagcgga agccgaaggg cggtggaatt tcatccgcga ccgcggaaac cgccggaacg
    12301 agaaggagcg cgacgagcgc tgccggcaag aagcgcatcg gtgcctagcg ttcacccaga
    12361 ttaaagacct cgtagcgccg gacaagcggt cgcagatagc ggcggatcgc ttcgagatgg
    12421 tgcgggtctt tttcgtaccg ttgcagggcg gcacgatcgc ggaaggtgat cagcacgcca
    12481 agatcaaagc tgcgatcggc gtccggacca agccccggca cggggcggcc ggtctccacc
    12541 cggagcacac ccggaatcat ctgcagcgac tgggccaccc ggataagttg cgcgcgcgcg
    12601 gcggggtccc cgggatgtcg caaccacacc agcaccacgc gggtcacggt cggcgaaggc
    12661 gccggtgtcg tgctgcaggc cgcgaagaat accgcgaaac agatcgcaag cacggtggcc
    12721 gcatccagcc tgccgcagtt atttcttcca gactgtctca tgcgttgctt tggtccacga
    12781 atttcgggca aatcgatcgc ttggcgaacg ttttcgcacc tgctcgcgag gcggctaaac
    12841 ctccccatga agtccgagca agctcgccgc cagactttgc ctaacaagtt gccgattcgc
    12901 cgaaaaatcc cgcacgagag aaaaacgggc ccacgccaga gaccattttg cgtcaaggtt
    12961 tttctgagcc tgcatgcaaa ataaagttcc gattttttct tgtgggccgg tgcgccgatt
    13021 tctaatctga agccgcaggc cgggaggaca gataccgggt gtgaacaaga tgtgaataag
    13081 ctgaacacct gaaatggaat cgaaagcatg aagaaaaatc agccgaattt ttgggccacc
    13141 actccgccag gttacctcgc cgggatgctg ccttccctgg atgacgtaaa agccagtttt
    13201 ccctcgtata acagaatttt gtgtcgaatt tcccctcgga cgaacgcagg aatggcctcg
    13261 tcgcacggcg tgccgcggag cgctttcatc ctgtcatcct ttctctccca gcttactgag
    13321 ggcgttattt aacgctgtta acaagtagcc atggacgaga agtgtaccca attgtggacg
    13381 aagctgtccg ccgccctcaa acccgaggtc agcgccgata cttttaagcg ttggttttcc
    13441 gccgtgaaat tgaccagcgc gaccgcggag gaggtgacgc ttctcgtccc gaacaacatt
    13501 tatcaattct ggatcgagag taatcacatg cccgctttgc aagccgcgat tgcctccgtg
    13561 gtcggcggcc caaggaccgt gaaatttgtc tgcgccgtcg atacctccgc cggctcggac
    13621 gcggccgacc cggaaccggc tgtttcgagc cgggcacaag cggcggcggg gcgagcgaac
    13681 aagacgccgg gactgaatcc gcgcaacaca ttcgaatcat tcgtggtcgg gcctaacaat
    13741 gagattgcgc acgcggcgag cctcgcggtc gcgcaatcgc ccgccaaaac ctacaatccg
    13801 cttttcatct atggcggcgt cggcctcggc aaaacgcacc tgatgcaggc gatcggtcaa
    13861 tacatcggcg cgcggggcaa gagcacgaga gtgatgtatc tctcgagcga gttgttcatc
    13921 aacgaattca tcgacgcgat ccagcacaac aacctggtca agttccgcaa acgctaccgc
    13981 caggccgatc ttctcctcat cgacgacatc catttcctcg ggggcaagga acgctcccag
    14041 gaggaatttt tccacacctt caacacgctt ttcgacggac ataaacagat tgttctctcc
    14101 agcgaccggc ccgcctcgga aatcgccaac ctcgaacatc gtctcgtctc ccgcttcgaa
    14161 tggggcctga ccgcggaact gcaaccgccc gatgtcgaaa cgcggatggc gattctgcgc
    14221 aagaaagcgc ggacgctgaa aatcaggctg cgcgatgaga ttttcgattt cctcgcaacc
    14281 cgcattaaga cgaatgtccg ccggctcgag ggcgcactca tgcgggtggc gtcctttgct
    14341 tcgctcagcg gcaaggagct gacccacgaa gtggtcgaac atctgctcaa ggatattttg
    14401 caggaggaag cgcgccgcgc ggttacgatc gaccagatcc agcgtcgcgt cgccgagcac
    14461 ttcgacgtgc gcctggcgga tatgacgagc aagcgccggc cggcgaacat cgccttcccg
    14521 cgacaaatcg cgatgtatct cgcgcgcgaa ctaacgaaag cttcgctcaa cgaaattggc
    14581 gaagcctttg gcgggcgcga tcatggcacc gtcctgcacg cctgcaagct ggtaaagaag
    14641 cggatgaagg aacaggacaa gatccggcag acgatctcat tcatcgatag cgcactgcaa
    14701 cgttgacgcg cgctgcgaga ttgcggttgc cggataaaat cgaaagcctt acatcttaac
    14761 gactcatctc cgccttcatg aaatttagcg ttacgaaaga gaagctgctc gagtgtctgc
    14821 aacaggttca aaatgtcgtc agcacgcgca cgacgctgcc gattctttcc aacgttctcc
    14881 tgcaagccaa tggcaaggag gtccgcctca cgacgaccga tctcgacgtc ggagtacgcg
    14941 gcagcttcga ggccgaggtg gaaaaagagg gcgccaccac cctgccggca cgccgtcttt
    15001 tcaccatcat ccgcgagctg ccctcgagcg aaatccagtt cgatatcgat ggaaagaacg
    15061 ccgcttcgat ccgcagcggg cagagtttct ttaagatcct cgggttaccg gaggaggagt
    15121 ttccgccgct cccgaagttc gacgattcca aagtcgtgac cattcgccag aaggatctgc
    15181 gcgacggact gcgcaagacc tcctacgcca tctcgaccga tgagacacgc tacgtcctca
    15241 acggcgtcct cttcagcttc aaggataaca agctcactct ggtcgcaacc gatggccgcc
    15301 ggctcgcgat gctcgatatc gatcttgaat ttccccgcag ccacgaaacc gacattatcg
    15361 tgccgaccaa ggcggtcacg gaattgcaac ggctcctcac ggacgagggc gacgtgcgag
    15421 tcagcgtcgg caccggccag atcgcgtttg atctcaacaa cacgctcctg gtttcgaaat
    15481 tgatcgaggg aaattatccc aactacaaac aagtcattcc cggcgagatg aaagagcgtg
    15541 tcacgctgga acgggaaact tttctcaact cgttgcgtcg cgtctcgctc ctcgccagcg
    15601 acaagtcgaa ctcgatcaag ctcaatttca ccaagaacaa catcgacatc actgcgaaca
    15661 cgccggaagt cggcgaagcc aaggaatcat tgccggtcgc ctacaaaggc cgcgagtttt
    15721 cgatcgcctt caatcccgaa ttcctcatgg cgcccctgcg gaatctcgcg gaagacgaaa
    15781 tctttctcga cctgatcgac gagatgagcc cgggcgtgat caagatccaa agcccgtttc
    15841 tttacgtcct gatgccgatg cgcatcagct cgtaggttgc ctttcgccgg ggacggatat
    15901 ctccccccgt ccccaggctt ttcattcgcg cctgctcccc ggacatgcgg aggcgcggct
    15961 ttccgcaggg gacatgcttc cgcatgtccg gtttgcggtg cgcgcagcgc ggagatgaat
    16021 gacgcgccgc acgctcaaca gatacgggag cggcgtcccc ggttagaggt gcgccaggac
    16081 ctgttccacg ctgattccgt ccatcgttag gccgggcgcc cgcaggacgc gcacctgtgc
    16141 attggccggc gcccagatcg ccggatcggt cggaccaaac aaaagaaggc acggcgtccc
    16201 gaccccggcc gcgaggtgcg agatgccgct gtcgtgcccg agaaacaagc cgcattcctg
    16261 aagacgcgcg gccagatccg ccagcgtaag ttcctgcgcg atctccacgc gctcgtcagg
    16321 caaagcggcg cggagctgcg gcgtacggcc atggtcggct tcgccgccga tgagaagcaa
    16381 ccgggtttta tcgttaggca agagaagcga ccgggcgatt tccacccagc gttccaacgg
    16441 ccaggtcttg ctttcactcc cgctgcccgg gtggatggcg atcaatgacg gatcccgtcg
    16501 ccgcccctcg ggtggcaaga tgatcgccgc tgggtcgtcc agataaagcc ccaggctctc
    16561 gagcgggcgc gcgagttgcc gggccgcgtg ctcctggtcg gtcaccttcg gcgaaccgac
    16621 gaggagatgc cgcaccccgg cgcgtttcag gttgtccgca aagatccggt cgggatcgaa
    16681 gagatagctg atcacctgct ggaagctgcc gaaataatcg accaggtcgg gaccgaggtc
    16741 gccgtcgcgg ctgaaaaagc tggcgagcga cgcggactca atcgagcggg tcgcattcgc
    16801 atagccgctc atcgtggcga gcaccgcgat atgcttgtaa ccgagaacct caaggtatgc
    16861 ggacgggaaa ccatgccgca gcaacttcag cgcgggcaaa gtgaggatga agtcgccgat
    16921 cgcgccgccg cgaatgacca gaatccgacc caaggattcg tcaataaacg aacaccgcgc
    16981 aggcgacaat caccgcgccg agggcgaagc cgagccccga aaccaaccgc catcgttccg
    17041 ccgttttcgt gctccagtgg atctggtcgc ggagcaagta gggcatcgtc acccaaaaca
    17101 atcccgccac gatcatcagg taagcgagca cggtgaccag cagtcggctc atctccggac
    17161 gcaaaaacgc cgcgtcgagc agcggctcgg ccgcgagcag acaaagaatc ccaagggcgc
    17221 gcacggcgag aaattcatcc acaaatttca gcacgagaac aaaaccgact ggcaaaatga
    17281 tgaggagcgg ccgccggaaa ttggagaact cgcccatctc catcgtcgcg aggagccaaa
    17341 agctccagat cagatcgacc gtgagcaacg cggcgccggc ggagcgcgag cgtggaaact
    17401 ggggcaggta attgcgggcc ggcaaaaaaa tcgcggccag gctgagcagg gcaagcaagg
    17461 cgccggcaat caagccgaca gcatacaaag agaagtggta gatcatgagg cgaccgcggc
    17521 gcgcggcaaa agttcttcca tcggcggcaa atgacaactg ccggcacgat ccattttcgt
    17581 ccccgacagg ctgaagcccg tcgagtgagt gatttgcaaa tcgacgagct gcccgatatc
    17641 ggcctcgctc cccgcgaaga ccacaatctt gtttgtccgc gcgcgaccca tgagccgcgc
    17701 ccgattcgtc ttgctcggcc cttcgcagag gatctgcaca cggcgcccga ccagccgctc
    17761 gcccgcacgc ttcgccgcgg catccacgat cgtgagcaaa tcctgattgc gcgcttcctt
    17821 gactcgctca tcaatctgcc cgcccatttc ggccgcgggc gtttcgcttc gctgtgagta
    17881 gcgaaagaca aacgcgttgt cgaattgcac ccgctccacc agggcgcgcg tcttttcgta
    17941 atcctgctcg ctttctcccg gaaacccgac aatcacgtcc gtcgtcagcg ccacgtcgct
    18001 ccgcatcgcg cgaatctggt cgaccagccg gagatatttg tccgcggtgt aggtccgatg
    18061 catcgccttg aggattcgat cggaaccgga ttgaagcggc aaatgcacgt gctcgcagag
    18121 tttcggcagg tcgcgcagcg ccgcgatcag atccgcgcga aaaccaatcg gatgcggcga
    18181 ggtgaaacgc aggcgctcga gtccgtcgac ttcgtggacc gcttcgagga gctgaacaaa
    18241 agggcttttg ccgtcgattc ttggaaactc gtgacggccg tagagattca cgatctggcc
    18301 gaggagcgtt acctccttca cgccccgcgc gatcagccgg cgaacttccc taacgatctc
    18361 cgcgatcgtc cggctgcgtt ccgcgccccg ggtccgcggc acaatgcaga aggtgcagtg
    18421 catgttacag ccctgcatga tggagacgaa ggcgctcgcc tggtgtgccc gcagagtgtg
    18481 gtctcggatg gtttcctgcg aacctgtttc ctcggccgtg tcgacgatgg aaaatcgtgg
    18541 gtcatccatt gcccggacgg cgcgctttcg ttccatcagt tcgtcaacat aatccgcgac
    18601 ccgatgaaac ttctgggtgc cgacaacgag gtcgagatgc gggattaact tgagcaattc
    18661 ggcgccacgc gcctgtgcca tgcacccgag caaaccgaag accgcgccgg gccggtcttt
    18721 tgccatccgc ccgagcatgc ccattttgcc gagcgccttc tgctccgcca tgtcgcgcac
    18781 gctgcaagta ttcaggagca cgacgtcggc ctcggcctcg ctcgccgccg cctcgtagcc
    18841 acggtcgagc aacgagcgcg cgacctgttc cgaatcgcgc tcgttcatct ggcagccgta
    18901 ggtcttgata aagaatttcg gcatcggagc gcggcaacga tacaccaatg tgccctcctc
    18961 gccatgccgc aggccgcctt aaatcgttag gtcgggttcg cagagccagc cgctaatacc
    19021 ggcgtgtttt tgtcctcatt atttttccaa cgcgttgcac tcgattatcc ccttcccaag
    19081 cgctcctcct tttgaataaa tcgccgcgcc aaatgaaatc agaaaatgca gccatcggga
    19141 ttatcggcgg gagcggcctt taccaaatcg aaggtttcag caatccgcgg gagcatcagc
    19201 tcgagacacc ttttggcacg ccgtccgata cgatcatcgg gggtgagatc ggcgggcggc
    19261 gggtctactt cctgccgcgg catgggcgcg gccatcgcct gctcccgcac gaactgaatc
    19321 atcgcgccaa tatctatgcc ctccgcgccc tcaacgtgcg ctggatcatc tgcgttaccg
    19381 cggtgggcag tttgcaggag aaatacgcgc cgcgcgacgt cctcctgcct tcgcaatttt
    19441 acgaccgaac aagccagcgc gcggaccaca cttttttcgg cgacggggtt gccgcgcata
    19501 tcacttttgc ggaaccgatc tgcacgccat tgcgtaacct gctcgcggag gttgccggtt
    19561 cgctaggcgt acgcgttcat aatggcggga cctatgtcaa catggatggc ccggcttttt
    19621 ccacccgcgc ggaatcggat ctgaatcgcc gccacggctt cgacgtcatc ggcatgacca
    19681 atctgccgga agccaagctg gcccgcgagg ccgagatcgc cctcgcttcg atggcgatga
    19741 tcacggacta cgactgttgg aaaatcgacg aagagccggt ctcggcccag acggtggtcg
    19801 ggcatttgct ggccaatgcc gagaccgcca aaaggattct tgccgtggtc atcccacgcg
    19861 tcccgacggc gccggattgg ccggagcatc gcgcgctcga ctccgcgttg gtcactgacc
    19921 aaaagctatg gcccccggcc acccgggaaa aattgcgcgc catcctcggg cggttcggat
    19981 aagcgcggga gggtagtcct cgaaggagaa gtgctgcgtg tattccgtgg aaaaaccttg
    20041 cgcaaagagt acttgcagcg gcgaatcaaa atatagtaat tatagggaag tcgaggcgag
    20101 cagtgatttc gcctcgtggc aaaggtaaaa aatgaaattc aaaatcgccc tcagcctgat
    20161 cgccgtggcc atgttgccgg cttgctcgac aaccaaaacc gcatcgaaca ccggcgacgc
    20221 aaagagctcg cgctacgcca ataattcggt cgcggaagcg gtcccgcccg ccgaagggcc
    20281 aaccgccgat attccctccg aaggtccgca tgacgtgatt gccaacccgg cctacatgcc
    20341 atcaccgctg cttcgcactt cggccgcggg cggcccctaa cgatggcggg atgttttgtt
    20401 gtcgccgggg tcgacgcccc gactacaacg cggttttcgt ctgctccgcc agacgcccga
    20461 tcgcttcgcc taacgaagtg atttgcgcct ccgaatggga ggcgctcacc gtcagccgaa
    20521 ggcgcgccgc gcctttcgcg accgtcggat aacgaatcgc cggcacgagg aacccctcct
    20581 tttgcagcgc ctgcgccaga tcgagtgctc gttgctcgtc gcccaccatc cagggaatga
    20641 tcgcggcgcc cggctggcca aactcttccc tcacctcggg gagcattttg cgaagctgca
    20701 caatccgcct ccaaagtgtg tggcgacgcg cctcgccttc ttcggaagcc agaaaatgaa
    20761 cggccgcggt tgcggcggcg gccagggccg gcggcggagc tgtggaaaaa ataaaggaac
    20821 gggcgcgatt gatcagccag tccacgaggc tgcgcgaccc gcagatgtaa ccgccgctca
    20881 cgcctaacga cttgctgagg gtgccgagct ggacgtcgat ctgcgcctcc aatcccagct
    20941 cggccgccag tccccgaccg tgcggtccga cgacgccgat ggcgtgcgct tcatcgagca
    21001 ggaggcaggc gtgaaagcgg ttcttcaact caactaacgc gcgcagtggg gcccggtctc
    21061 catccatgct gaagactgat tccgttacga tcacgacacg ggcctgggga tgctcccgcc
    21121 gcgcccattc gaggtggctc tcgagtttgc cgaggtggtt gtgcggaaaa atccggattg
    21181 tggcgccgct caggcgggcg ccatcgatca aggaagcgtg acagagctta tcgaggatca
    21241 cgacatcccg cttcgacgcc agggcaggca acgtcccgac cgctgcggca tatccgctcc
    21301 cgaagcaaag cgcagccggc gcgcgtttcc acttcgcgag cgcggtttcc aaggcgacat
    21361 gaggcgattg cgttccacta atcaggcgcg atgctccggc tcccacgccg aactcttcga
    21421 tcgccgcgct cgccgcccgg cgcagaatcg gatcagccgc cagcccgaga taatcgttcg
    21481 atgaaaagtt gacgagatgg cgcccgacga tctgcacttc gggtccctgc gcggtgccga
    21541 tctcgcgcaa cttgcgatgc agtgattgcg cgcgcagcgc ctccagttgc tcgcgaaaaa
    21601 gatcgctcat gcgcgcggcc gccgcgtgag ctacatccgc tggagacccc agtaaagcaa
    21661 cgcggacgcg tcccgccaga tgccggccga actgtcgcca aggacgacaa caatgatgtc
    21721 gcgtcccgga atcttaccgc tggcaatgag gcagtgaccg gccgcttcgg tgtagccggt
    21781 tttcatccca ttgcagtagg ggacctgctt gaggactttg ttcgtgttct tgagttcgcg
    21841 cacgcggcca ttggcataac ggaaggcgag ctgcggcaga caaacaatcg accgaatggt
    21901 tggattcgca taggcggcgc gcgcaatgag ggaaagatcg cgcgccgtcg aatactggtc
    21961 gggaatcggc aggccgttgg gattcacgaa attcgagtgc gtcgcgccga gggcgcgtgc
    22021 cttggcattc attttttcag caaacaattc gatgctgccc gcgttatccc gcgccagaca
    22081 acgcgccacg tcgttcgggc ttttcacgag cagcgcccgc agcaactcga tgcgggaata
    22141 gctctcaccg ggcttgatat tcaaccgcac cggctccgcc tgggtgtcga tcagttgcac
    22201 cgtcacgttc tgatcgagaa aaccgtcctc cgcgacgatc agggcggtca gcaatttctg
    22261 ggtgctcgcc gcggcccgcg gcgcgtccgc gtttttctcg tagagaatct cgccggaacg
    22321 cgcgtcctgc accaggactg cggcggcccg cgttctgggc gggccgttga ggcccttcaa
    22381 aagctcgtgc gtctgatagc caccggacga atcctcgtcg tccgtgacgg cttgcgcccg
    22441 tttcaccgga agactgttcg gctgggtcgc ggcgacgcag agcagcgcca gcgcgcaggt
    22501 caacggaaat gtcaggcagc cagtagctcg acggatcatt gacaggcaat tagagagcaa
    22561 aagacggcgc cgcgcaagac taacgaccgg ccccgcgctg aatcaactcg acctcgtatc
    22621 cctccggcgc gtcgataaaa gcgatcgcgc cgccgcgccc attgggatgc gggccgtcgg
    22681 agagcgggta acccttcgcg gccgtttgtt tcgcgaattc ctcgatgttg tcgacctcga
    22741 aggccaggtg ggtcaggtca ggcccgacca ccaccggacc gctttcgtcg aacttgcaga
    22801 gctcgatttc ctcctcgctc tccggtgcct tgaggaagac gagttgcgac ccgcggccgg
    22861 aggtatgccg gcgaacctct tccagtccga gcacatcccg gtaaaacgaa acggtcttct
    22921 ccaggtcctt cacccgataa cgtgtgtgca gaagcttctt gatcatcgct atcttattgc
    22981 gccggatgcc gattaatttc aaattcggcg cacttcgcag atcctctcca tgctttctct
    23041 cggaatcgat agcggcacca aaagcacgaa atcgctcgtc ctcgatctca catcggggga
    23101 agtcctcgcg ctggcgcagc gcagctacgg cacgatcgca ggcctgccgc ccggtcacgt
    23161 ggaacaagat ccgaaaattt ggatcgaggc cgcggaagcg acggtcgcgg aatgcctcga
    23221 aaaggtcggg ccgcgccgcg cggaaatcag gtcggtcggg gtgagcggac agcagcatgg
    23281 gctggtgccg ctcgacgaaa cgaacgagcc gttgcgcccg gccaagctct ggtgtgacgt
    23341 ttcgaccgag tcgcagtgcg agcaattcaa caaggaattt ggcggcggcg caggcctgat
    23401 cgaacgcctc ggtaatccga tgctcccggg ttacactgcg ccgaagcttc tttggctgaa
    23461 agaaaacgag ccggctaatt tccgccgcct aacgaccgtc ctgctgccgc acgattacct
    23521 caacctctgg ctgaccggcg aacgccagat ggaatacggc gacgcctccg gcaccggcct
    23581 gatggatgtg cgcacccgca cctggtgcaa accgatcctg aaattcatcg atccaaagtt
    23641 ggaatcgacg ctgccgccgc tgcgctcctc gaaacgaccg gccggtttgt tgcgcagtgt
    23701 cctgcgcgaa aaatggggcc tggccgacga cgctctggtc agcgccggga gcggcgacaa
    23761 catgatgggc gcgatcggca cgggcaatat tcgcgcgggc gtggttacca tcagcctggg
    23821 cacatcggga acgatctgcg cgtttgcgga agagccagtc gtcgacctga agggcgaggt
    23881 cgccgccttc tgcgacggca ccgatcactg gctgccgctg acctgcacga tgaacgtggc
    23941 ggtcgcgacc gagcaaatgc gcgcgctctt tggctgggat attcccacca tggagcaacg
    24001 cgtcgccgcg gtgcccgcgg gcgcgggcgg cgttcttttc ctcccctacc tgcagggcga
    24061 gcgcactccg aacctgcccc gaagctccgc cgttttccac ggcctaacga cggccaacat
    24121 gacgcccgat ttcatggcgc gcgcggtggt ggaaggtgtg acgctgggcc tcgcctacgg
    24181 cctccgccgc ttccaggatc tcggcatcgg gccgacggag attcggctca ccggcggcgg
    24241 aagcaagagc gcggtctggc gccagatcgc cgccgatatc ctcggctttc ccaccgtcgc
    24301 gctgcgggtg acggaaggcg cggctttggg cgcggcgatt caggcggcgt ggacctattc
    24361 cgaggtcaaa ggccaaccac tgagcctcga agaactggtc gacgatctcg tgaaggttga
    24421 caaaaaaaca agggccgagc cgcgccaaga agctcagatg ttttatcacg agctcctgcg
    24481 gcggcggatc gatctgacga agaaactgaa gaccagcgga tacctttagc ggttcgcttc
    24541 tcgtgtagaa acgagaccgc attggcacgg tccggacagg cggaagcatg tccctccgcg
    24601 ggcacgcgct tccgcgcctc cggtctccct ccgagggatg gcggtcccgg tttggccgat
    24661 acagtgcact agagtttgcc tgcaacttgt gaaacgttag gcaaaatgaa acttgtcatc
    24721 gccgcgacgg gcgccagcgg cacgctctat ctccagcgtt tactcgagca gatcgacacg
    24781 cgcgcgaacg aagtgcacct ggtgctgagc ggtcacgcga aacaggtcgc cgccaccgaa
    24841 ctggaacgcc tggtcgtacc ggacggggtg cggcagcatg gcgagaatga tttgcatgtg
    24901 cccttcgtca gcggctccgc ccgcttcgat gccatggtca ttgtgccctg ctcgatggcg
    24961 acgctgggcc gcgtcgcctg cgggtcgggc gacagcgcgc tcctccgggc ggcggacgtt
    25021 tttctgaagg aacggcgcaa gttgatcgtg gttcctcggg agacgccgtg gagtttgatc
    25081 cacgcgcgca acgtcgtcac cctgctcgag gcgggcgcga tcgtgctccc ggcgatcccc
    25141 tccttttaca gccggccgca atccgtgaac gacttggtcg acaccgtcgt ctggcggatt
    25201 ctcgatcaga ttggcctgcc gaatccgcgc gcctatcgct ggaaggacga gagcgcgccc
    25261 agctccgagc attgaaaatc gaaggctggc gcgcgcggtg gctgatcgct ttcggttatc
    25321 ggctgctcca aatctgggcg cgcaccctgc gtttcacgat cgaggaccac gccggcgtcg
    25381 tcggtgcccc acctaacgag cgctacatag gcgccctctg gcataaccgg ctgctccttc
    25441 tgcctttcgt cctcaagcgc ttcctcccgg agcggcgcgg cgccgcgctg atcagcgcca
    25501 gccgcgacgg cgcgctcctc gccgacctgg tggagcgctt cgatttcgag gtcgttaggg
    25561 gatcgacttc gcgccagggc gcgagcgcca tccgccaact cgccgaaatc atcgcgcgcg
    25621 ggaaggacgt cgttatcaca ccggacggcc cgcgcggtcc ggcctacgaa ctcggccagg
    25681 gcatcgttta tctcgcccag caatcgggtg ccgcggtcgt gccgatcaac ctggaatact
    25741 cgggctcttg gcgggtgaaa agttgggacc gcttcattct gcccaggccc ttttccaggg
    25801 tgcgcgtcat tttcggcgcg ccgcatcgcg tcgcgcagac ctcgaccgaa gcagacttcg
    25861 agcgcgaacg cacgcggttg caggaagcga tgatccagct agtggagatg cgatgaagtt
    25921 gatcgatggc cggacaattg ccgaaaaagt ttacgccgag ctgcgcggcg aaatcgcggc
    25981 gctgaaagca cgcggggtca cacctggact cgcggtcgtg ctcgtaggcg atgatccggc
    26041 ctcgcgcgct tacgtccgct cgaaggacaa gatgtgtcgc gaactgggcc tgcattccgt
    26101 caagctcgaa ttgccggcgg aaacgacgca gtcggatctc ctcgcgcgcg tcggcgattt
    26161 gaatcgcgat cccgcgattc acggcattct tgtccaatcg ccaccaccca ggcagatcga
    26221 tgaggctgcg atcatccgcg cgctcgaccc ggcaaaagac gtcgatggtt ttcacccgat
    26281 gaacatcgcg aaactcgccc tcggcgatgc gaccggtttt gttccctgca cgccgctcgg
    26341 ctgcctgcgc ctgctgacgg aaagcggcgt caccattagc ggcgcgcatg tcgtcgtgct
    26401 tggacgaagc atgatcgtgg gcaaaccgct cgctcttctc ctcatgcaaa aaggaacgga
    26461 tgcgacagtc acggtggtcc actcgcgcac ccgcaatctg gcccagatca cgcgcacggc
    26521 ggatgtcgtg gtcgcggcga tcggccgggc cggattcgtc agggcagaac acgtgcgcga
    26581 aggcgcggtg gtgatcgacg tggggatcaa ccgggtggag gacgcggccg cgccgcgtgg
    26641 ttaccggctg gtgggcgacg tcgccttcga taaagtggcg ccgaaaaccg ccgcgatcac
    26701 ccccgtgcca ggcggagtgg gacccatgac gatcgcgatg ttgatggcca acacggtcaa
    26761 agccgcacaa cttttgtcgt cagccgaccc agcattcgct tctcgttagg cagataacgg
    26821 agcagaccgg tcgtcaggcg cggatgcagg accgcgctgc gggagaggag gttgatccaa
    26881 aggcgcccgg cgtaaagccg gcggtgcgcg gccgcgtagc gccggtggtc cttcgcgccg
    26941 atcgcggccg cggccagttc gccggaccga agcgcatagt agattccctc gcccgtgaac
    27001 ggctcgacca ctcgcgccgc gtcgccgaca agaaacaagc cgggctgact cgcgggcagc
    27061 gccgcccgag ccagcggtgc gatcgtccgc caggcgtgat caacggggat cgagaactgc
    27121 cgctcggccc agtgcctgat cgccggcaaa tcgcgcggcc ggctcacgag gcaaaggttg
    27181 agcagaccat cgccaaccgg cgcctggccc gaatagccct cggccagcag ttgcaggaca
    27241 acacggtcgc cgaagtccag cggcagcggg acatgtgtct gcagcgccac gcgatcgcgc
    27301 cctttgcgcg gcatcaggtt gctcaaacgg gcgaccgtcg agttccgccc gtcggccgcg
    27361 accaggacag gcgcgcgctc cacggattgt tccgttgtga tttgccaatg ctcgccgcgc
    27421 gtgaccgaaa gcagcgtctc cccttcccgc acttccgcgc ctaacgatcg cgcgcggcgc
    27481 agcagaattt ggtcgaggat actgcgcttg atggcaatct ccgcatcctt tcccgcaggc
    27541 agcggcgccg tcaccgaccg gccatctagg ccgatgaatt ctaccatcga caactgtcca
    27601 tgcggcgcgc ggcgcacttc cacggacacg ccgagccgat cgagcacggg ccagcaagcg
    27661 gggttcaggc aatcgccgca gactttctcg cgcggaaaat gtgcgcgctc gaggaccagc
    27721 gtccgcagcc cagcctgcgc gcaaaacgcc gcgcagctcg cgcccgccgg gccactgccc
    27781 acgatggcga catcccacat tttcgagtac tcttccacga ccgtgccgct gtcgaaggaa
    27841 aagccagacc tgaacaagaa agtgcacgag gtgccgcaca agcccggcgt ttacctgatg
    27901 cgcgaccggt tcggccgcgt catttacgtg gggaaagcgc gcgacctgcg caagcgcgtc
    27961 ggctcctatt tcctgccctc gaaaatggcg gtggccgatc tgaagacgcg cgcgctcctg
    28021 gaagcggtct gggatttcga aatccacacg gtggcgagcg aaccggagtc gattctgctc
    28081 gagggcaagc tgatcaagga atatcggccg cgttacaaca tttcgtttcg cgacgacaag
    28141 cgcttcctgg tggtaaaggt cgatcccacg gaggaatggc cgcgttttcg tttggcgcgc
    28201 ttcaagaaag acgacggggc gcgctacttt gggccgtatg cgcacgccgg cgcgctccgg
    28261 cagacactca atttcatgcg caaaaaattc ggcgtactca cgttcgggcg cggctcgccc
    28321 acggaacgcg aattgaaatc gtcgacctat caggtgccca cccgcttgag cgagatcaac
    28381 gcggaaacct accgggatcg cgtcgcgcag gccgcggaat ttctcgaggg tcattcgcgg
    28441 gaaatgatga acgcggtcga ggaggagatg cagaaagcgg ccgccaagca ggatttcgag
    28501 aaagcggccg agctgcgcaa catgctcgat gatttgcggc gcacaacgag gccgatgcga
    28561 cgcttcacgc ggcacagcct gccaagcacg atcgatccgc aggccgacgt gcgggcgctg
    28621 gccgacgcgt tgcggctgcc gaatttgccg gccgtgatgg agtgcttcga catctcgaat
    28681 at
//
LOCUS       DATMNS010000291        20274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3823695, whole genome shotgun sequence.
ACCESSION   DATMNS010000291 DATMNS010000000
VERSION     DATMNS010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 20274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20274
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3823695"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VNW28_10350"
     CDS             complement(<1..962)
                     /locus_tag="VNW28_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10374.1"
                     /translation="MTRQRQAAQRGRGPGAPIWRLTIAAWCACAPLATEAAIAAQPSP
                     EPVAELSAQRAAAERGKIYQAGVVYSQWIDGLAQESGSPFLQQRVLDRVTWMRLLACA
                     GTLLVVGLATAWFLRFVRRRAGRIESDEQQSWLALGAAAICKPLALLAWVIGGFLSLM
                     PIAEGIASRPTRLFLANGLTAILYAGWVIAVFWLIFQIIRGAEKRMRQWAQGTGKVLN
                     NVIVPVAGQTLRLAVPLLAILLFLPLLNLPDRWTWVTQKGFGILLIVSLASLIIRGVR
                     AVQVALMREHRLDVEDNLAARRIYTQVSVIRKIVIAAVVILATGS"
     gene            complement(959..1348)
                     /gene="acpS"
                     /locus_tag="VNW28_10355"
     CDS             complement(959..1348)
                     /gene="acpS"
                     /locus_tag="VNW28_10355"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HXA10375.1"
                     /translation="MSIVGLGIDLVDCARIENSIERFGERFLKRVFTEGEIAYSQSMK
                     FPARHFAARFAAKEALSKAFGTGIGKAMGWRDLDVQKKESGEPFVVLSGGAKQMAETL
                     GVARVWISLSHTEASGMATIILEGRDG"
     gene            complement(1345..2097)
                     /locus_tag="VNW28_10360"
     CDS             complement(1345..2097)
                     /locus_tag="VNW28_10360"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HXA10376.1"
                     /translation="MGILRLGVNIDHVATLRQARYAAMPDSKNAEPDPVAAASLCERA
                     GAGGITAHLRADRRHMQDRDLERLRANIMTKLNLEMGNTTEIIDFALLLQPDEVCLVP
                     EQRQEITTEGGLDLVRHGWELAPTIKRLHAAGIRVSLFIDPAPEQVDAAVKLETDMVE
                     LHTGALSNAFTEKIEQQELERLRAAAMRASKFGLQVNAGHGINYRNIALIHKIPHLVE
                     LNIGHSIVARALFVGIETAVSEMLGLMQNYRG"
     gene            complement(2104..2727)
                     /locus_tag="VNW28_10365"
     CDS             complement(2104..2727)
                     /locus_tag="VNW28_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10377.1"
                     /translation="MARSENQPPGRASGLPLLLCAIWFVVALFAGASGRMAELRPPAP
                     QVVLLGLTLALILAYNLSRSFRNWTSQWNLRALAALHLTRFVGLYFLVLNDRGELPRA
                     FALPAGLGDIAVATLALILINRRPTAERRRLYFLWNSLGLIDILFVVATAARLSLRDP
                     ASMAALSRLPLSLLPTFLVPCIIASHLLIFVRLRQPSGLPSSSNHPY"
     gene            complement(2740..3657)
                     /locus_tag="VNW28_10370"
     CDS             complement(2740..3657)
                     /locus_tag="VNW28_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXA10378.1"
                     /translation="MSVLVVGSIALDTVKTPVEEHADQLGGSASYAAVGASFFAPVQL
                     VGVVGEDFPASEFAFWKSRAIDASGVQRAPGKTFRWSGEYAWDLNTRETKSVALNVFE
                     NYKPELPESFRATEFVLLANIAPSLQAHVLDQMRRPRFVVADTMDLWIETTRPDLDAL
                     LPRVDLLILNDSEARQMTGETSLIRAGRALRRMGPKYVAVKKGEHGALLFGPDDFFSC
                     GAYPLEDIHDPTGAGDTFAGGLAGYLAGECAGEVNFALLRKALIYGSVLASFNVEAFS
                     LDRLRRLTSEEIADRYQMFKLMSQFEVLE"
     gene            complement(3661..4329)
                     /gene="thiE"
                     /locus_tag="VNW28_10375"
     CDS             complement(3661..4329)
                     /gene="thiE"
                     /locus_tag="VNW28_10375"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXA10379.1"
                     /translation="MDSLARCQLYAILDLGYVAPGDAAAVTAELIRGGADVIQLRGKS
                     QTIAELTRLADELHGLTSHAGVPLIINDHPEIARDLPVEGLHLGQDDLAIPAARAIVG
                     RDCGVGKSTHSLAEAIAAVAEGADYIGFGPVFATPTKPDYRAIGLGQIRRVHEQVQIP
                     IFCIGGIKIENLSDVLAAGATRVVIVSGLLQGKDVAAATRAAKQLLEQNQKSVRLRRI
                     SNQQ"
     gene            complement(4340..5725)
                     /gene="accC"
                     /locus_tag="VNW28_10380"
     CDS             complement(4340..5725)
                     /gene="accC"
                     /locus_tag="VNW28_10380"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HXA10380.1"
                     /translation="MFEKILIANRGEIALRVIRACKELGIRTLAVYSEADVDSLHVQL
                     ADESICIGPAASSDSYLRIDRIMSAAEVGDVDAIHPGYGFLAENAHFAEVCESCNIKF
                     IGPTPAAMHAMGDKNSARAAARKAGVSVTPGSDGIVENEAEAVRVAKKIGYPVMIKAV
                     FGGGGRGMRAAHNEVSLIQGFHGARLEAEKAFGNAEVYIEKLILNPHHIEFQIMADHH
                     GNTVHLGERDCSIQRRNQKVIEECPSPLLTPALRKRMGHAAIKLAQSVGYTNAGTMEF
                     LVDQKGNYYFIEMNTRIQVEHTITEEVYGCDLVKEQIHIAAGDKLSPHVARPTARSHA
                     IQCRINAEDPARNFQPSAGKITFYYAPGGRGVRIDSHVYTGYSVPPFYDSMIAKLIAV
                     GATRGSAIARMRRALGEYLITGIKTTIPFHNAIMRNADFRNGNYDTGFVDRIMNSETF
                     ELRPSTGGFRD"
     gene            complement(5792..6259)
                     /gene="accB"
                     /locus_tag="VNW28_10385"
     CDS             complement(5792..6259)
                     /gene="accB"
                     /locus_tag="VNW28_10385"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HXA10381.1"
                     /translation="MELKDIKAIIDLMRKNDLSVFEMEKDGFRLKLQKGAGEQTVYAA
                     PVLPQMITAPAQPGAVAHAGEAAPAAPSAPTAAATKEITSPMVGTFYRAASPESPPFI
                     EVGREVNEDTVVCIIEAMKVMNEIKAETSGVITEVVAENGKPVQFGQALFKVR"
     gene            complement(6429..7112)
                     /locus_tag="VNW28_10390"
     CDS             complement(6429..7112)
                     /locus_tag="VNW28_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXA10382.1"
                     /translation="MISLQDVNRYYEAGERSVHALDHVSLEIARHEFVAVVGPSGCGK
                     STLMHLIAGLDRPTAGEISVDGLSLTTADDAALTQFRRRQLGLVFQFFNLLPTMNVRE
                     NVSLPLLLQGVPIAGATARADELLQLVGLTNRATHFVHQLSGGEQQRTAIARALVHRP
                     SLLIADEPTGNLDTASATRVLALLRQIADEQLATLILVTHSAEVAAAASRLIEMRDGR
                     IVSDRMAAL"
     gene            7111..7947
                     /locus_tag="VNW28_10395"
     CDS             7111..7947
                     /locus_tag="VNW28_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HXA10383.1"
                     /translation="MPASAHLPTAKAHARTPPKFRIIAESPDYLVVDKPPFLLIHPNK
                     PDGTPTLWAALRELLAFELVNGGQVSIVNRLDRETSGLVLIAKHAVTARRFGLAMQAQ
                     LVAKEYLAIVCGWPAWETTMVDAPLARQGAHGPSAIWLKQAIHEKGAPAVTEFRVEKR
                     FVRPGNPKDRWAVVRAFPRTGRTHQIRVHLAHLGHPIVGDKIYGPDEGHYLRFIETGW
                     TDELASQLFLPRHALHSARISLEGEFDWQSPLPEDLQNWMRSSPHCHPERSAPEPKNS
                     VD"
     gene            complement(8016..8678)
                     /locus_tag="VNW28_10400"
     CDS             complement(8016..8678)
                     /locus_tag="VNW28_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10384.1"
                     /translation="MKIICTLFLLVVCTFRSGLALADENAGNAAQLAAAVWKASGGEN
                     WPNVRAIDFTFAVEKGGQTVVSAEHHWDVAAQTDHVKWKDKDVTVNLLSPAADENAKA
                     AYGRWVNDSYWLLAPVKLKDHGVHLASAGNKQIDGAEREVLHLSFGTIGLTPNDQYNL
                     YIDPATKLVTSWDYMPKPGESMHSTWADYEESGGLKLATDHKMDGGVRIRILHLKVTA
                     AK"
     gene            8747..9373
                     /locus_tag="VNW28_10405"
     CDS             8747..9373
                     /locus_tag="VNW28_10405"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HXA10385.1"
                     /translation="MKPSLALALCAALLFATKAQAGDAPAPEAKTAALESDDFEVPNL
                     EFAAESAYLLGIFGNPNSYEIGSEFITARFRWGVNRNEDSVFRGYNQVYVLGLAEPIF
                     RGPENHYFGISTGFRHVFIRPGWRLEPYISGGVGLGFIDSTDAFRQGQGQDFTFNILS
                     AAGLTYRLDDHWKLSLGILYQHLSNGGQTDPNPSLNLLGPQVGATFSF"
     gene            9388..10002
                     /locus_tag="VNW28_10410"
     CDS             9388..10002
                     /locus_tag="VNW28_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10386.1"
                     /translation="MTPPNENVLVVRRSLLDQLGGFHGLNFEPQPYLDAILARGNNYF
                     LARATAENDPTHKQIIPYALLVCGDRVLHYVRGKKAGEQRLVAKGSIGIGGHMNDEDE
                     SLFAWDEAAYRAGVEREVNEEVLIETPFEDRIVALLNDDTTEVGRVHVGVVHVFRLRE
                     TKVQKREAMITNLAFLTKAELLERRETLETWSQICVDQLDRLLA"
     gene            complement(10132..10962)
                     /gene="ispE"
                     /locus_tag="VNW28_10415"
     CDS             complement(10132..10962)
                     /gene="ispE"
                     /locus_tag="VNW28_10415"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXA10387.1"
                     /translation="MQLFAPAKINLSFQIRGRRTDGFHEIETLMTPIALCDELTIEPD
                     DLGAGLAFSSDDPSLPAGEDNLVVRAARSFFAEIHAEPAVRIALRKKIPHGAGLGGGS
                     SDAASTLLGLNELHGRPLPSARLTSLAAGIGSDVPFFLAQGAARCRGRGEIVEPVSPP
                     PELPLLLLKPEFGVPTSWAYAQWRDSREVPGVDYTPQRVGELSLQNDLERPVFEKYVF
                     LARMKTWLRDQPEVGAALLSGSGSTIFAVLRAAEAAAALADRARIELDPGLWSCATSL
                     "
     gene            10991..11698
                     /gene="pyrF"
                     /locus_tag="VNW28_10420"
     CDS             10991..11698
                     /gene="pyrF"
                     /locus_tag="VNW28_10420"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF012441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HXA10388.1"
                     /translation="MNWQATEMNPRARLIIALDFPTSEAALALVRRLSPHAGLFKIGL
                     QLYTAAGPEIVRAVRDLGGRIFLDLKLHDIPHTVGRAVESADSLGVEMLTIHLSGGRA
                     MIEAAAGATKPEMLLLGVTVLTSLDPATLREIGIDAPLEEHVLRLARLGVESGIGGVV
                     ASPHEVVPLRRAHGSKIKIVTPGIRPRDAAADDQRRAMTPADAFSAGADFLVIGRPIT
                     AAQNPRAALEQIVADAV"
     gene            11688..12350
                     /locus_tag="VNW28_10425"
     CDS             11688..12350
                     /locus_tag="VNW28_10425"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HXA10389.1"
                     /translation="MLFERLILHDDRARASAAVNMAIDEALLEHSTLPVLRFYGWQRP
                     SLSFGYFGKFADIAGELEKHDVVRRWTGGGSVLHGEDLTYSLVTPAEDPASTHGPSAI
                     YAALHGAIRDALRMEGRETELAAHAAPKISDACFANPVRDDVLFRGRKIAGAAQRRTR
                     GGFLHQGSIQVAELSETFRERLAAALAPEIRRAEIPPQTLDRATTLAAEKYGTDDWLR
                     RW"
     gene            12614..13639
                     /locus_tag="VNW28_10430"
     CDS             12614..13639
                     /locus_tag="VNW28_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXA10390.1"
                     /translation="MKITANSAVTANGVAGPLSRVFAPVQPQLDEVDRRISDQTAAFD
                     PAIEGYVAYAIGGGGKRLRPLVALLSGGATGALASGHIDLAIIVELIHLATLVHDDIM
                     DEAERRRSQPTVNARWGNSLSVLLGDVLFAHALNLSTDFDDTHISRTIARTATEVCSG
                     EMIQTQRRFDLQLSRGEYFRIIGMKTGSLFACAAELGAVISGAPDGVVRRMKNYGAKI
                     GTAYQIYDDCLDIAGTESETGKTLGTDLRKGKLTLPVLTLLESASVFDRERCCALVLD
                     GKLAEVTLLLKNSPADGALGASIDTAAELIRDAQAELASLAANRYVDSLFALGEALHE
                     LLEQLRG"
     gene            13709..14380
                     /gene="radC"
                     /locus_tag="VNW28_10435"
     CDS             13709..14380
                     /gene="radC"
                     /locus_tag="VNW28_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HXA10391.1"
                     /translation="MPKEDRPREKLAARGAGALNDAELIAILLGTGLQGANAVEVARQ
                     LIKEYGSLGTLSRCSVQELARIKGIGFAKATQLAAAFGLGHRLARENLARKKIDTPER
                     VYELLGPEMRALHKESLRIILLDTRYSLIRVEDVSLGSVNESIAHPRDVFRPALIYSA
                     YAVVVVHNHPSGDPAPSQADHGLTRRLREAAELLQIRLLDHIIIGAPAEGRLPYFSFK
                     ETGVL"
     gene            14377..15180
                     /gene="lpxA"
                     /locus_tag="VNW28_10440"
     CDS             14377..15180
                     /gene="lpxA"
                     /locus_tag="VNW28_10440"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR01852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HXA10392.1"
                     /translation="MKIHPTALVDPGARLGDDVEVGPFSCIGGEVEIGARTIIQSHAV
                     IEGMVRLGADNQVGHGALIGGPPQDLGFKPGTRSAVEIGDGNIIREHVTIHRGTAAGS
                     ATRVGHHNFLMAGAHLGHNCTLGDKVIIANNCLLGGYVTVENGAFLGGGCVFHQFMRV
                     GQLAITQGTSGFGKDIPPFVAAAGINQVVGLNVVGLRRSEFDPEERAEIRKAFRLLYE
                     SGLNVSQALTQARERKWREPAQAFFDFVAGAKRRGICGLRRTPGPSGED"
     gene            complement(15184..15516)
                     /locus_tag="VNW28_10445"
     CDS             complement(15184..15516)
                     /locus_tag="VNW28_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10393.1"
                     /translation="MDQESHNQPTALARPPTYHGFWPVLLMGLSLVLVFSWEIRVGIA
                     TRQGAQQLQEQQARLVDEAKRVQTGLEKIVRGLVDLSKTDDAAKKIVTQFGIKVNNPS
                     IPTATPAP"
     gene            complement(15519..15797)
                     /locus_tag="VNW28_10450"
     CDS             complement(15519..15797)
                     /locus_tag="VNW28_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10394.1"
                     /translation="MKRWQFVLLLTLGVVCLCLSLVTIVFAQENRKLQEALQMQQAAI
                     NKGSLSQQIGTNLLREMAATAQTDEKMRDLLQQNGFSLPAKSTPTPTP"
     gene            complement(15867..16538)
                     /locus_tag="VNW28_10455"
     CDS             complement(15867..16538)
                     /locus_tag="VNW28_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HXA10395.1"
                     /translation="MDARAADPAVEFFPALRDLADVRHAFIGRIPGIDVATDRAEALA
                     RLDAAHRALRANLGFVGGAFATAEQVHGNTIGILDEPLRTDQCLHGADGLVTNQEGVS
                     LGIYVADCCAIFLVDPVRRCIGLVHSGKKGTALAIVSRAIRQMGERFGSVAHDLVVQL
                     SPCIRPPHYEIDFAAEIIRQARAAGVEEVRDSGRCTACEPRRYYSYRAEKARTGRMLA
                     LLALK"
     gene            complement(16547..18166)
                     /locus_tag="VNW28_10460"
     CDS             complement(16547..18166)
                     /locus_tag="VNW28_10460"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXA10396.1"
                     /translation="MSRDPAGAVILSAFQRIECFCRTRIFSSPQRRQAASVLGLLILA
                     STLFAGQSAETSREKATSPGARVFTLRKAIETAFQRNPAILTARQEIRRTKGVQIQVL
                     SQALPHLDATAAFNYTDPSLNNSSGGSLITPGGTPSPTAAPTATPVSSALKAATISNP
                     STSFSTTADSYNLRLTASQLLYNGSVIPAIKGAGSASDASLYALRNTIDQVIATVREQ
                     FYTVILNKELIGVQEESVSLLESQLKDQQNRFEAGTVPRFNVLQAEVALANQQPALIT
                     ARNNYRIAQIQLGRTIGLDFDPRRGDKAPLECVGELAYEPRDIPLPVAIELGKERRPF
                     LKQQRANILVQVQQLRGAFAGIQPSLSVNGGYAFESSEFSDSLSAVATGWFFGITGSW
                     AIFDGLQTVGAVKSARATLSEAKITYDDSVRQVELEIQQAYSNLQQDRELYASQSKNV
                     DQAREALRLATARLGVGAGVQLDVLNAQLALTQAQSTRLSALAAYNSDLAEFDRATAT
                     DTIYHDDFDDPMMHKRGETRMTTTTGTSSPK"
     gene            complement(18163..18660)
                     /locus_tag="VNW28_10465"
     CDS             complement(18163..18660)
                     /locus_tag="VNW28_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HXA10397.1"
                     /translation="MSHSLGVPKMGAATAYLTTATDELRGDAERLAHIIMEMQRCFIL
                     NLSKELAPGHVSFPQFFLLTYLDLKEVLTMSAIAQKMGHTTAAASGLVARLENLGYVM
                     RSHASDDRRKVMVCITPRGSALVRRIREEMICNLMKMMAHLSPGEQKSWLQIYSKIYD
                     YCQNK"
     gene            complement(18866..>20274)
                     /locus_tag="VNW28_10470"
     CDS             complement(18866..>20274)
                     /locus_tag="VNW28_10470"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HXA10398.1"
                     /translation="NVIEWVHRSTRGWSYGTSIIDPRTGEIIKGHVSLGSLRVRQDFL
                     IAQGLVEAYANGATPDPRLLELSLARLRQLAAHEVGHTLGLQHNFAASTQNRASVMDY
                     PPPLVSIDSKGNIDLSQAYATGIGAWDKRTILYGYQEFPSEADEAAGLASILQENIAR
                     GFRYLTDADARPPGSANPFAHLWDSGDSATDELNRLIKVRALALNRLSEKNIPPGAPL
                     ATLENVLVPVYLMHRFQAEAATKLIGGVNYTYATRGDGQTTNEPLPPEQQRAALTAVL
                     QTLRPEFLEMPAKLVALLPPRPPGYPRDRESFDSHTGLVFDPLAAAESWINAELDLLL
                     NPQRLSRIVAQDAAAVHGLSLNELFDAILLAGERTAQQTGPQKEIARAVEKQFLNHLL
                     PLALERDTEPQVNAYAMRRIADLEAKWKARSANDPAEMAQNAYLLAQIDQFHRDPKAL
                     EIPMPPRIPDGSPIGADE"
BASE COUNT         3840 a         6394 c         6283 g         3757 t
ORIGIN      
        1 gagcccgtag cgaggatgac gaccgccgcg atgacgatct tgcgaatgac cgagacctgg
       61 gtgtaaatcc ggcgcgcggc gaggttgtcc tccacatcca gccgatgctc ccgcattagc
      121 gccacctgga cggcgcgcac cccgcgaatg atgagggagg ccagactgac gatgagtaga
      181 atgccgaagc ccttttgcgt cacccaggtc cagcgatccg gcaggtttaa aagcggcagg
      241 aacaaaagga tggccaggag cgggacggcc aggcggagcg tctggccggc caccggcacg
      301 ataacgttgt tgagcacctt gcccgtgccc tgcgcccact gccgcatccg tttttccgcc
      361 ccgcggatga tttggaaaat caaccagaaa accgcgatta cccagccggc gtagaggatc
      421 gcggtcaacc cattggcgag gaagagtcgc gtcgggcgcg aggcaatccc ctccgcgatc
      481 ggcatgagcg aaagaaaacc gccgatcacc caggcgagca gcgcgagcgg cttgcagatc
      541 gccgccgccc cgagagcgag ccaggactgt tgttcgtccg actcgatacg gcccgcgcgc
      601 cggcgcacga accggagaaa ccaggcggtc gccaggccga caaccagcag cgtcccggcg
      661 caggccagga gccgcatcca ggtcacgcga tcaaggacgc gctgctgcaa aaacgggctg
      721 ccgctttcct gcgcgaggcc gtcgatccac tggctgtaga cgacgccagc ctgataaatt
      781 ttgccgcgct ccgccgcggc gcgctgggcc gagagttccg cgaccggttc cggagaaggc
      841 tgcgcggcga tcgccgcctc cgtggcgagt ggcgcacacg cgcaccacgc tgcaatggtt
      901 aggcgccaga tcggggcacc agggcccctc ccacgttggg ccgcttgtcg ttgccgggtc
      961 atccgtcgcg accttcgagg atgatcgtgg ccatcccgct cgcttcggta tggctcagac
     1021 tgatccagac cctggccacg ccgagcgtct cggccatttg ttttgcgccg ccgctcagga
     1081 cgacgaaggg ttcgccactc tctttttttt ggacgtcgag atcgcgccag cccatcgctt
     1141 tgccaatccc ggtgccgaac gctttcgaga gcgcctcctt ggccgcgaat cgcgccgcga
     1201 aatgacgcgc gggaaatttc atcgattgcg aataagcgat ctcgccttcg gtaaagacgc
     1261 gcttgagaaa gcgctcgccg aagcgctcga tcgaattctc aatccgggcg cagtccacca
     1321 gatcgatccc aaggccgaca atgctcatcc ccggtagttt tgcattaacc cgagcatttc
     1381 gctcaccgcg gtctcgatcc cgacgaagag ggcgcgggcc acgatcgagt ggccgatgtt
     1441 cagttccacg aggtgcggga ttttgtgaat gagcgcaatg ttgcggtagt tgatgccgtg
     1501 accggcgttg acttgcagcc cgaatttcga cgcgcgcatc gcggccgcgc gcagtcgctc
     1561 cagttcctgt tgctcgattt tttccgtgaa agcgttggag agcgcgccgg tgtgcaactc
     1621 caccatgtcc gtctcgagct tgaccgccgc atcgacctgc tcgggcgcgg ggtcgatgaa
     1681 cagactgacc cgaatcccgg cggcgtgcag gcgcttgatc gtcggtgcca gttcccaccc
     1741 gtgcctaacg agatcgagac cgccctcggt cgtgatctcc tgacgttgct cgggcacgag
     1801 gcacacttca tcgggctgca gaaggagcgc gaaatcgatg atctccgtgg tgttgcccat
     1861 ctcgagattg agcttcgtca tgatattggc gcggagccgc tcgagatcgc gatcctgcat
     1921 atggcgacga tcggcacgga gatgcgccgt gatcccgccg gcgccggccc gctcgcacag
     1981 actcgccgcc gccacgggat cgggctcggc attcttcgag tcgggcatcg ccgcgtagcg
     2041 ggcctgccgg agagtcgcca cgtgatcgat attaacgccc aggcgcagga ttcccatctg
     2101 gattcaatag gggtggttgg acgaagaagg caaacccgaa ggttgcctta gcctaacgaa
     2161 aataaggagg tgcgaagcga tgatgcatgg aaccagaaac gtgggcagaa gactcaaggg
     2221 caggcgcgag agcgctgcca tcgaagccgg atcgcgcaag gacaggcgcg ccgcggtcgc
     2281 gaccacaaac aagatgtcaa tcaagccgag cgaattccac aggaagtaga gtcgtctccg
     2341 ttccgccgtc ggccgccggt tgatcaagat gagcgcgaga gtagcgacgg cgatgtcacc
     2401 aagaccggcc ggcagagcaa acgcgcgcgg caattcgcca cgatcattga gcaccaaaaa
     2461 gtagaggccg acgaaacgcg tgagatgcag cgcagcgagc gcgcgaaggt tccactgcga
     2521 agtccaatta cgaaacgagc gcgagagatt gtaggcgaga atgagcgcga gcgtcagacc
     2581 gaggagcacg acctgcggcg cgggcgggcg caactccgcc atccggccgc tcgcgcccgc
     2641 aaacagcgca accacaaacc agatcgcgca gaggaggagc ggcaggcccg atgctcttcc
     2701 cggcggttgg ttttcggatc gcgccatgct gcggcgaacc tattcgagca cctcaaactg
     2761 gctcatgagc ttgaacatct ggtagcggtc tgcaatttcc tcactcgtta ggcgacgcaa
     2821 gcgatcgagg ctgaaggcct cgacgttgaa gctcgccagc acactgccgt aaatgagcgc
     2881 cttgcgcagt aaggcaaaat tcacttcgcc tgcgcactcg cccgcgaggt agcccgccag
     2941 gccgccggcg aaggtgtccc cagcgccagt cggatcgtga atgtcttcca atggataagc
     3001 gccgcagctg aaaaaatcgt cgggaccgaa gagcagcgcg ccatgctcgc ccttcttcac
     3061 cgcgacgtat ttcggcccca tgcgccgaag cgcgcgtccg gcccggatca ggctcgtttc
     3121 accggtcatc tgccgtgctt cgctgtcgtt cagaatgagc agatcgaccc ggggcagcag
     3181 ggcgtcgaga tccgggcggg tggtttcaat ccaaagatcc atcgtgtcgg caacgacgaa
     3241 ccggggccgc cgcatctggt cgagcacatg tgcctggagc gacggcgcga tgttggcgag
     3301 gagcacgaat tccgtggccc gaaaactttc cggcagctcc ggtttgtaat tttcgaagac
     3361 attcagcgcc acggactttg tttcccgcgt gttcaaatcc cacgcgtact cgcccgacca
     3421 gcggaaggtt ttgccgggcg cgcgctgcac gccgctcgcg tcgatcgcgc gcgacttcca
     3481 gaaggcaaac tccgatgccg ggaaatcttc gcccaccaca ccgaccagct ggaccggcgc
     3541 aaagaaactc gcgccgaccg ccgcgtagga agcggaaccg ccgagctgat cggcatgttc
     3601 ctccaccggc gttttgacgg tatcgagcgc gatggagcca acgacgagga cggacatgga
     3661 ttattgctga ttgctaatcc gcctgaggcg gactgatttc tgattttgct cgagcaattg
     3721 cttcgccgcg cgggtggccg cggcgacatc cttgccttgc aggaggccag atacgatgac
     3781 gacgcgcgtt gcgccagcgg ccagaacgtc cgacaaattc tcgatcttga tcccgccgat
     3841 gcagaaaatc gggatctgca cctgctcatg cacgcgccgg atttggccga ggccaatcgc
     3901 ccgatagtcc ggcttcgtcg gggtggcgaa aaccgggccg aaaccaatgt agtccgcgcc
     3961 ctcggcgacg gccgcaatcg cctcggccag actgtgggtc gatttcccga ccccgcaatc
     4021 gcgccccacg attgcacgcg ccgcggggat cgcgagatcg tcctgcccaa gatgcaggcc
     4081 ttccaccgga aggtcgcgcg cgatttccgg atgatcgttg atgatcaacg ggacgccggc
     4141 atggctcgtt aggccatgca gttcgtccgc gaggcgagtc agctcggcga ttgtctggct
     4201 ttttccgcgc agctgaatca cgtccgctcc accgcgaatc aactccgcag tgacggcggc
     4261 cgcatcgccg ggcgcgacat agccgagatc gaggatcgcg taaagctggc agcgcgcgag
     4321 tgagtccacc tcggcacgct caatcgcgga aaccaccggt tgatggccgc agctcgaaag
     4381 tctcggagtt cattatccgg tcgacaaacc ccgtgtcgta attgccattg cggaaatcgg
     4441 cgttgcgcat gatcgcgttg tgaaacggga tcgttgtctt gatgccggtg atgagatact
     4501 cgcccaacgc cctccgcatc cgggcgatgg ccgacccgcg ggtcgcgccc accgcgatca
     4561 gtttcgcgat catcgaatca taaaaaggag gaacgctgta gcccgtgtaa acgtgggagt
     4621 cgatccgtac cccgcgaccg ccaggcgcgt agtaaaacgt gattttgccg gccgacggtt
     4681 gaaaattgcg cgctggatcc tcggcattga tccggcactg gatggcgtgc gagcgcgccg
     4741 tcggccgggc aacgtgcggc gaaagtttgt cgcccgccgc gatgtgaatc tgttccttga
     4801 ccaaatcgca gccgtagact tcctcggtga tggtgtgctc cacctggatg cgggtgttca
     4861 tctcgatgaa gtaataattt cccttctgat cgaccaggaa ttccatcgtc ccagcgttcg
     4921 tgtaaccgac cgattgcgcg agcttaatcg cggcgtgccc catccgcttg cgcaaggccg
     4981 gggtgagcaa gggcgacgga cattcctcga tcaccttctg gttgcgccgc tggatcgagc
     5041 aatcgcgctc accaagatgc accgtgttgc catggtggtc ggccatgatc tgaaactcga
     5101 tgtggtgcgg gtttaagatc agcttctcga tataaacttc ggcgttgccg aaagctttct
     5161 ccgcttccaa ccgggcgcca tgaaatccct gaatcaggct cacttcatta tgcgccgccc
     5221 gcattccccg accaccgccg ccgaagaccg ccttgatcat caccggataa ccgatcttct
     5281 tcgcgaccct gaccgcttcc gcttcgttct cgacgatgcc gtcgctgccc ggcgtgaccg
     5341 agacgccggc cttccgcgcc gccgcccgcg cggaattctt gtcacccatc gcgtgcatcg
     5401 cggcgggcgt cgggccgata aacttgatgt tgcagctctc gcagacctcc gcgaaatgcg
     5461 cgttctccgc cagaaatccg tagcccggat gtatcgcatc cacgtcgccc acctccgccg
     5521 cgctcatgat gcgatcgatc cttaggtaac tgtcggagct cgccgccggg ccaatgcaga
     5581 tcgattcatc cgccaactgc acgtggagcg aatccacgtc ggcttcggaa tagacggcca
     5641 gcgtgcggat gccgagttcc ttgcaggcgc ggatgacgcg gagcgcgatc tcgccgcggt
     5701 ttgcgatcag aattttttcg aacatgaaaa agtgaggcga gcccgtcgct cagtcaaaac
     5761 gcaagctgga agccggcgcc aaattcttcg cctaacgaac tttgaaaagt gcctggccga
     5821 actgcaccgg tttgccgttt tccgccacca cctcggtgat caccccgctc gtctcagcct
     5881 tgatctcgtt cattactttc atcgcctcga tgatacagac gacggtgtct tcgttcactt
     5941 cccggccgac ctcgatgaac ggtggagact ccggcgaagc cgcgcgatag aacgtcccga
     6001 ccatcggcga agtgatctct ttggtcgcgg cggcggtggg cgcgctcggc gccgcgggcg
     6061 ccgcttcccc ggcgtgcgcc actgctcccg gttgggccgg ggccgtgatc atctgcggaa
     6121 gaactggcgc ggcgtaaacc gtttgttcgc ccgcgccttt ctgcagcttg aggcggaagc
     6181 cgtccttctc catttcgaaa acggagaggt cgttctttct catcaggtcg atgatggctt
     6241 tgatgtcttt aagttccaaa ggcgcgtcct ttccggcgtt ggtgagacgc taggtaaagc
     6301 ggttttgacg gcggggtcaa gcaaccgttc gcgccccgat ctggagacgt ttcgctctgc
     6361 gaaacgcggg gaaagcgcgg ccgacagccc aagggtaggt tttcccgtgc cccgcccaaa
     6421 gggcggcgtc acaatgcggc catccggtcg ctcacgattc ggccgtcacg catttcgatc
     6481 agccgggaag cagccgccgc gacttccgcg ctgtgggtaa cgagaatcag tgtcgccagt
     6541 tgctcgtccg cgatctggcg caagagcgcc aggacccgcg tcgccgaggc ggtgtcgaga
     6601 ttgccggtcg gttcgtcggc gatgaggagc gacggccggt gaaccagggc ccgcgcaatc
     6661 gccgtccgct gctgctcgcc gccgctcagt tgatggacga agtgcgtcgc ccgattcgtc
     6721 aggccgacaa gctgcaggag ttcgtccgcg cgcgcggtcg ctcccgcgat cggcacgccc
     6781 tgcaggagca gcggcaggct gacattttcg cgcacgttca tcgtcggcag gagattgaaa
     6841 aactggaaaa ccagcccgag ctggcgccgg cgaaattggg tcagagccgc atcgtccgcc
     6901 gtcgttaggc tgaggccgtc gaccgaaatc tcgcccgcgg ttgggcgatc gagcccggca
     6961 atcaggtgca tcagggtgct cttcccgcac ccgctcgggc cgacgaccgc gacgaactca
     7021 tgccgcgcga tctcgaggga cacgtggtcg agcgcgtgga ccgagcgttc gccggcctcg
     7081 taatagcggt taacgtcttg cagagagatc atgccggcgt ccgctcatct acccacggcc
     7141 aaggctcacg caaggacgcc gccgaaattc cgtatcatcg ccgagagccc agattacctc
     7201 gtggtcgata agccaccctt tctcctcatt catccgaaca agcccgacgg cacgcccacg
     7261 ctctgggccg cattgcgcga actgcttgcc ttcgagcttg tgaatggcgg gcaggtttcc
     7321 atcgtcaacc gcctcgaccg cgagacgagc ggccttgtct tgatcgcgaa gcatgcggtc
     7381 accgcgcgcc gtttcggtct cgccatgcag gcgcaactcg tcgcgaagga atacctcgcc
     7441 atcgtgtgcg gctggccggc ttgggaaacc acgatggtcg acgccccgct cgcgcggcag
     7501 ggtgcccatg gcccttctgc catttggtta aaacaggcga tccacgaaaa gggcgcgccg
     7561 gcggtgacgg aatttcgggt cgaaaagcga ttcgttaggc cgggcaaccc gaaggatcgc
     7621 tgggccgtcg tccgtgcgtt tccgcggacc ggtcggacgc atcagatccg ggtccatctc
     7681 gcccatctcg gtcacccaat tgtcggcgac aagatctatg ggcctgacga ggggcattac
     7741 ctgcgcttta tcgagaccgg ttggacggat gaactggcga gccagctctt cttgccgcgg
     7801 cacgcgctgc attcagcgcg gatttctctg gaaggcgaat tcgattggca aagtccgctg
     7861 ccggaggacc tgcaaaactg gatgcgttcg tccccgcact gtcatccgga gcgaagcgca
     7921 ccggagccga agaattccgt ggactgacct ttaaggtcgc gcgacgggat cctcgactgc
     7981 gcgcgggatg acggaccgtc cagagtggca cgcgcctact tcgccgccgt caccttgaga
     8041 tgcagaatcc ggatgcgcac gccgccatcc attttgtgat ccgtcgccag cttgagcccg
     8101 cccgattcct cgtaatcggc ccaggtgctg tgcatcgatt cgccgggctt gggcatgtaa
     8161 tcccatgagg tcacgagttt cgtggccgga tcaatgtaga ggttatattg gtcgttaggc
     8221 gtgagcccga tcgtcccaaa actgaggtgc agaacctcgc gctcggcgcc gtcgatctgc
     8281 ttgttaccag cgctcgcgag gtggacgccg tggtctttca gtttgaccgg cgcgagcagc
     8341 caataggaat cattcaccca gcgcccgtaa gccgccttcg cattctcgtc ggcggccggg
     8401 ctgagcagat tcacggtcac atccttatcc ttccatttca cgtgatcggt ctgcgcggcc
     8461 acgtcccaat gatgctcggc cgagaccacc gtctgaccgc ccttctccac ggcaaaggtg
     8521 aaatcgatcg cccgcacgtt aggccaattc tcgccgccgc tcgccttcca gaccgccgcg
     8581 gcgagctgag cggcgttccc ggcattttca tccgccaaag caaggcccga acggaaggtg
     8641 cagaccacga gtagaaagag agtgcaaata attttcatga ctgcaacatg acgatgcgcc
     8701 gcccggattg caattgcccg aagccgaaat atggttcggc ccgttgatga aaccttcgct
     8761 cgcgctcgcg ctctgcgccg ccctcctttt cgcgacgaag gcccaggctg gcgatgcccc
     8821 ggcaccggag gcgaagaccg cggctctgga gtcggacgat ttcgaggtgc cgaatctgga
     8881 gtttgccgct gagtcggcct atctgctcgg cattttcggc aatcccaaca gctacgagat
     8941 cgggtccgaa tttatcaccg cgcgttttcg ctggggggtc aatcgcaatg aggacagcgt
     9001 atttcgcggc tacaaccagg tctatgttct cggcctggcg gaaccgattt tccgcgggcc
     9061 ggaaaatcat tacttcggta tctcgaccgg ttttcgtcac gtcttcatcc ggccgggctg
     9121 gcgactcgag ccctacattt ccgggggtgt cggtctcggc ttcatcgaca gcaccgatgc
     9181 tttccgtcag ggccagggac aggatttcac gttcaatatc ctgagcgccg ccggcttgac
     9241 ctaccggctg gacgaccatt ggaaactcag cctgggaatt ctctaccagc acctctccaa
     9301 tggcgggcaa accgacccga atccgagcct caacctcctc ggaccgcagg tgggcgccac
     9361 tttttccttc tagggacatc cgccgacgtg actccgccta acgagaacgt actggtcgtt
     9421 aggcgaagcc tcctcgatca actgggcggc ttccacgggt tgaacttcga gccgcaacct
     9481 tacctcgacg ccatcctggc gcgcgggaac aactatttcc tcgcgcgcgc cacggccgag
     9541 aacgatccga cccacaaaca gatcatcccc tatgccctgc tcgtctgcgg cgatcgcgtc
     9601 ctgcattacg tccgcggaaa aaaagccggc gaacagcgtt tggtcgcgaa aggctcgatc
     9661 ggaatcggcg gtcacatgaa cgacgaggac gaatctctct tcgcctggga cgaggcggcc
     9721 taccgcgccg gagtcgaacg cgaggtgaac gaagaggtgc tgatcgaaac cccgttcgag
     9781 gaccggatcg tcgcgctctt gaacgacgac acgacggaag tcggacgcgt ccacgtggga
     9841 gtcgtgcacg ttttccgctt gcgggaaacg aaagtgcaaa agcgcgaggc gatgatcacg
     9901 aacctcgcct tcctcacgaa agccgaattg ttggagcgcc gcgaaacgct cgaaacgtgg
     9961 tcgcaaatct gcgtcgatca gctcgatcgg ctcctggcct aacgattttt ttcgggagca
    10021 catcgttgcc gggttgcaga cacttcgcgc tgcgcagcgc cgcggaaaat gtcgctcgcc
    10081 tcccacagct gcgggcgaga actccgcccc gcgctcccga cagggaggcg tctacaacga
    10141 tgtagcgcag ctccacaggc ctggatcaag ctctatacgc gcccgatccg cgagcgcagc
    10201 ggctgcctcc gccgcgcgca agaccgcgaa gatggtcgag cccgaaccgg aaagcagcgc
    10261 cgccccgacc tccggttggt cccgcagcca agtcttcatc cgcgcgagaa acacgtattt
    10321 ctcaaagacc ggccgctcga gatcattctg caaactcaac tccccgaccc tctgcggcgt
    10381 gtagtccact cccggcacct cgcgcgaatc gcgccattgc gcgtaggccc acgaggtcgg
    10441 caccccaaac tccggtttca agaggagcaa aggcagttcc ggcggcgggc tgaccggctc
    10501 gacgatctcc cctcgcccgc ggcagcgcgc cgcgccctgc gccagaaaaa aagggacgtc
    10561 cgatccaatc ccggccgcga gactcgttag gcgggcggac ggcaacggac ggccgtgaag
    10621 ttcattcaaa ccgagcagcg tgctcgccgc gtcgctgctc cccccgccca gcccggcacc
    10681 gtgcggaatc ttcttgcgca gcgcgatccg caccgcgggc tccgcgtgta tttccgcaaa
    10741 aaaactgcgc gcggcgcgga ccaccagatt gtcctcaccc gccggcaggc tcggatcatc
    10801 cgaggaaaag gccaggcccg cacccaggtc gtcaggctcg atcgtcagct catcgcagag
    10861 cgcgatcggc gtcatcaaag tctcgatctc gtgaaagccg tccgtgcgcc ggccccgaat
    10921 ttgaaatgat aaattgatct tcgcgggcgc gaaaagctgc ataggtttcc gcaaccctgt
    10981 gtggaacggc atgaactggc aagccactga aatgaatccc cgcgcgcgcc ttatcatcgc
    11041 gctcgatttt ccgacgagcg aagcggcgct ggcgcttgtg cgccggcttt ctccccatgc
    11101 cgggctcttt aagatcggac tccagctcta caccgccgcc ggtccggaaa tcgtgcgcgc
    11161 ggtgcgcgac ctcggcgggc ggattttcct cgatcttaaa ttgcacgata tcccacacac
    11221 ggtcggccgc gcggtggaga gcgcggactc gttaggggtg gaaatgctga cgattcacct
    11281 gagcggcgga cgcgccatga tcgaagcggc ggcaggcgcg accaaaccgg aaatgttgct
    11341 cctcggagtg acggtcctca ccagcctcga tcccgcgacg ctgcgcgaga tcggcatcga
    11401 tgcgcccctc gaggaacacg tgctgcgact cgcgcgcctc ggtgtcgaaa gcggcatcgg
    11461 tggcgtcgtg gcgagtccgc acgaggtggt tccgctgcgc cgggcgcacg gttcgaaaat
    11521 caaaatcgtc acgcccggaa ttcggccgcg tgacgccgcg gcggatgatc aaaggcgcgc
    11581 catgaccccg gccgacgctt ttagcgcggg ggcggatttt ctcgtcattg ggcgtccgat
    11641 cacggccgcg cagaatccgc gcgcggcgct cgagcagatc gtggccgatg ctgtttgagc
    11701 ggctgattct gcacgacgac cgcgcgcgag cgagcgcggc cgtgaacatg gcgatcgatg
    11761 aagcgttgct cgagcattcg accctgcccg tcctccgctt ctacggctgg cagcggccgt
    11821 cgctctcgtt tggctacttc gggaagtttg ccgacatcgc cggcgaattg gaaaaacacg
    11881 atgtcgttag gcgctggacc ggcggtggca gcgtcctgca cggcgaggac ctgacctact
    11941 cgctcgtaac cccggcggaa gatcccgcct cgacccacgg accctcggcc atttacgccg
    12001 cgctccacgg cgcgattcgc gatgcgctcc ggatggaagg gagagagaca gaactcgccg
    12061 cccacgcggc gccaaaaatc tccgatgcct gtttcgccaa ccccgtgcgc gacgatgttc
    12121 tcttccgggg tcgcaaaatc gccggggccg cgcaacgccg gacacgcggc ggttttctcc
    12181 accagggcag catccaggtg gccgaactga gcgaaacatt ccgcgagcgc ctggcggcgg
    12241 cgctggctcc tgagatacgg cgggcagaaa tcccgccgca aacgctcgac cgtgccacca
    12301 cgctggccgc tgaaaaatac ggaaccgacg actggctgcg ccgctggtaa cttctccggg
    12361 gagcgcgcgc tactcgcgcg ttcggttccg cgacccgccg aacggctctt ctgcggcggg
    12421 cacggatgcc tcgccgtcgt tgcgccaggc gccgcgtcga gacgttttcg gcgagtcgcc
    12481 gaaaacagca cgcgggtcgc gggcgctccc cggagttttc ctgcgacgct gcggttgaat
    12541 ttccctccgt ttcccgagac gaatgctggt gaagcagctt tgacattaaa aactgacctt
    12601 ctatcctgcg cttatgaaaa tcaccgcgaa ttcagccgtc accgccaatg gcgtggcggg
    12661 cccgctgagt cgcgtctttg ccccggttca gccacagctc gatgaggtgg accgtcgcat
    12721 ctcggaccag accgcggcgt ttgacccggc gatcgaaggc tacgtcgcct acgccatcgg
    12781 tggcggcggg aagcgcctgc gcccactcgt tgcgctgctc agtggaggcg ccaccggggc
    12841 gctggcttcg ggtcacatcg atctggcgat catcgtcgaa ttgattcacc tcgcgacctt
    12901 ggtgcacgac gacatcatgg acgaggccga acgccgccgc tcgcagccga cggtcaatgc
    12961 gcgctgggga aattcgctca gcgtgctgct cggagacgtg cttttcgccc atgcgctcaa
    13021 tttgtcgacc gacttcgacg acacccacat cagtcgcaca atcgcgcgta ccgccacaga
    13081 agtctgctcc ggagaaatga tccagaccca gcgccgtttc gacctgcagc tgtcgcgcgg
    13141 cgaatatttt cggatcatcg ggatgaaaac cggatcgctc ttcgcctgcg cggcggaact
    13201 gggagccgtc atcagtggcg cacccgatgg agtcgttagg cgaatgaaga attacggcgc
    13261 caagattgga accgcctacc aaatttacga cgactgcctc gacatcgcgg gcaccgaaag
    13321 cgagaccggc aagaccctcg ggaccgatct gcgcaaggga aagctcaccc tgccggtcct
    13381 cacgctcctg gaatccgcct ccgtcttcga tcgcgaacgc tgttgcgcgc tcgtcctcga
    13441 cgggaaactc gccgaggtca cgctgttgct caaaaattcg ccggctgacg gcgcgctcgg
    13501 cgcctccatc gacacggccg cagaattgat ccgggacgcc caggccgaac tcgcgtcgct
    13561 cgccgcaaac cgttatgttg attcgctctt cgccctgggc gaagcgctgc acgaattgct
    13621 cgagcaactg cgtggctgaa agcccggcca ggcggacgcc gcaacttccc cgacgagcga
    13681 gatgacggcg cagctcaaaa ttcgtgagat gccgaaggag gatcggccgc gcgaaaaatt
    13741 ggccgcgcgc ggggccggcg cgttgaacga cgcggaactg atcgcgatcc tcctgggcac
    13801 aggcttgcaa ggcgcgaacg cggtcgaagt ggcccggcag ctgatcaagg aatacggatc
    13861 gttgggcaca ctcagtcgct gttcggtcca ggagctcgcc agaatcaagg gaatcggatt
    13921 tgcgaaggcg acgcaactgg cggccgcctt tggcctcggc catcgcctcg cgcgggagaa
    13981 cctggcccgg aaaaaaatcg acacgccgga gcgggtttac gaattgctcg gacccgagat
    14041 gcgcgccctg cacaaggaat cgctccgcat catcctgctc gacacgcgtt actccctcat
    14101 ccgggtggag gatgtttcgt taggcagcgt caatgaaagc atcgcgcacc cgcgtgatgt
    14161 cttccggccg gcgctcatct actcggctta cgccgtcgtc gtagtgcaca atcatccctc
    14221 gggcgatccc gcgcccagcc aggcggatca tggcctaacg agacgtttgc gggaggcagc
    14281 cgagctgctc cagatcaggc ttctcgatca tatcatcatc ggggcgccgg cggaggggcg
    14341 gctcccctat ttcagtttca aggaaaccgg cgtgctgtga agatccatcc caccgccctg
    14401 gtcgatcccg gcgcacgcct cggcgatgac gtcgaggtcg ggcctttttc gtgcatcggc
    14461 ggtgaagtgg aaatcggagc gcgcactatc atccagtcgc acgccgtgat cgaaggcatg
    14521 gttcgcctgg gggcggataa ccaggttgga cacggtgcgc tcatcggtgg ccctccgcag
    14581 gatctcggtt tcaagcccgg gactcgcagc gcggtcgaaa tcggcgacgg caatatcatt
    14641 cgcgagcatg tgaccattca tcggggcacg gccgcgggtt ccgcaacccg cgtcggccac
    14701 cacaatttcc tcatggccgg cgcgcatctc gggcataatt gcaccctcgg cgacaaggtg
    14761 attatcgcga acaactgtct cctcggcggt tacgtgacgg tggaaaacgg cgcgtttctt
    14821 gggggcggct gcgtattcca tcaattcatg cgcgttgggc agctcgcgat cacccagggt
    14881 acttccggat ttggcaaaga cattccgccg tttgtcgccg cggctggaat caaccaggtg
    14941 gtcgggctga atgtcgtcgg cttacgccgc tctgagttcg acccggaaga gcgcgcggag
    15001 ataaggaagg catttcgtct gctttatgaa agcggcctga acgtgagcca ggcgctcacc
    15061 caagcgcgcg aacgaaagtg gcgcgaaccg gcacaggcgt tcttcgattt cgtcgccgga
    15121 gcgaaacgcc gcgggatctg cggattgcgg cgaactcctg gaccgagcgg cgaagattaa
    15181 cgcctaaggc gcgggagtgg ccgtcggaat cgacggatta ttcactttaa tgccgaactg
    15241 ggtgacaatt ttcttcgccg cgtcgtctgt cttggaaagg tcgaccagcc cgcgcacgat
    15301 tttctcgaga ccggtctgaa cgcgcttcgc ctcgtcgacc aggcgcgcct gctgctcctg
    15361 caattgctgg gcgccttgcc gggtggcgat gccaacccgg atctcccagg agaaaacgag
    15421 gacgagactc agtcccatca ggagcaccgg ccagaagccg tgataagtcg gagggcgcgc
    15481 gagcgcggtc ggctggttgt gagattcctg gtccatcgtt agggggtggg cgtcggggtg
    15541 ctcttggccg gaagagaaaa gccgttttgc tgcagcaggt cgcgcatttt ttcgtccgtt
    15601 tgcgcggtcg cggccatctc ccgaagaagg ttggtgccga tttgctgact gagcgatccc
    15661 ttgttgatcg ccgcctgctg catttgcagc gcttcctgca gtttccggtt ttcctgggcg
    15721 aagacaatgg tcaccaggct gaggcaaagg cagaccacgc cgagcgtaag caaaaggaca
    15781 aactgccagc gtttcattcg cctaacgaat accagaggag acgccggagc gtcaacccga
    15841 aaagcaacgg gcggcgcccg ccggcgttac ttcagggcga ggagcgcgag catccggccg
    15901 gtgcgcgctt tctccgcgcg ataggaataa tagcgtcgcg gctcgcaagc ggtgcaacgg
    15961 ccggagtctc gcacttcttc cactccggcc gcacgcgctt gcctgatgat ttcagccgcg
    16021 aaatcgattt cgtaatgggg cggacgaatg cacgggctca gctggacgac gagatcgtgc
    16081 gccacggagc caaatcgttc gcccatttgc cggatcgcgc gtgagacaat tgccagggca
    16141 gtccctttct ttcctgaatg gacgaggccg atgcaacgcc ggaccggatc aaccagaaaa
    16201 atcgcgcaac aatccgcgac ataaatccct aacgacacgc cttcttgatt ggtgaccagg
    16261 ccgtccgcac catggaggca ttggtcggtg cgcagtggct catcgaggat gccgatcgtg
    16321 ttgccgtgca cttgttcggc ggtcgcgaaa gcccctccca cgaaaccgag attcgcccgc
    16381 agggctcggt gcgcggcatc caaccgagcc agcgcctccg cgcgatcggt ggcgacgtcg
    16441 atccccggga tacgcccaat gaaggcgtgc ctgacgtcgg ccagatcgcg caacgccggg
    16501 aagaactcca ccgccggatc cgcggcccgg gcgtccatca ccgacgctac ttcggcgacg
    16561 acgtacccgt tgtggtcgtc atccgtgttt cgccgcgttt gtgcatcatc ggatcgtcga
    16621 aatcgtcgtg ataaatcgtg tccgtggccg tcgcgcgatc gaattcggcg aggtcgctat
    16681 tgtaagcggc gagtgccgag agccgggtgg actgcgcctg ggtgagggcg agttgcgcgt
    16741 tcaaaacatc caattgcacg ccggcgccca ccccgagccg ggcggtcgcc agacggagcg
    16801 cttcgcgggc ctggtcgacg ttcttggatt ggctggcgta gagttcgcgg tcctgctgga
    16861 gattggagta ggcctgctga atctccagtt cgacctgccg aacagagtcg tcgtaggtga
    16921 tcttcgcctc gctcagcgtc gcccgcgccg atttcaccgc gccgacggtt tgcaagccat
    16981 cgaagattgc ccagctgccg gttatgccaa agaaccagcc ggtggcgacg gcgcttaggc
    17041 tgtcggaaaa ttctgagctc tcgaaagcat agccgccgtt cacgctcaga ctcggctgga
    17101 taccggcgaa ggcgccccgc aactgctgca cctggacgag aatgttagcg cgttgctgct
    17161 tgagaaaagg ccggcgttcc ttgcccagct cgattgcaac cggcagcgga atatcgcgcg
    17221 gctcataagc cagctcgccg acacattcga gcggcgcttt atcgccccgc ctcggatcaa
    17281 aatcgagtcc aatggttcgc ccgagctgga tctgggcgat gcggtaatta ttccgcgctg
    17341 tgattaatgc cggttgttgg ttggcgagcg cgacttccgc ctgcaggacg ttgaaccgcg
    17401 gaacggtccc cgcttcaaaa cgattctgct gatccttcaa ctggctctcc aaaagactga
    17461 ctgattcttc ctgcacgccg atcagctcct tgttcaagat gacggtgtag aactgctccc
    17521 gcacggtcgc gatcacctgg tcgatggtat tgcgcaaggc atagaggctg gcgtcgctgg
    17581 ccgacccggc gcccttgatg gcgggaataa ccgagccgtt gtaaagcaac tgcgaagcgg
    17641 tcaaacgaag gttgtaggag tcggcggtgg tgctgaagga tgtcgacgga ttacttattg
    17701 tggctgcctt aagcgcggac gacaccgggg tggccgtcgg agccgcggtg ggagacggcg
    17761 tgccacccgg cgtgatgaga gatcctccgc tactgttgtt taaactcgga tcggtgtagt
    17821 tgaatgcggc cgtcgcgtcg aggtgcggca aggcttgcga gagcacctga atctgcaccc
    17881 ctttggtgcg ccgaatctcc tggcgcgccg ttaagatggc gggattgcgc tggaaagccg
    17941 tttcgatcgc ctttctcaag gtgaagacgc gagccccggg ggaggtcgcc ttttcgcgcg
    18001 aagtctctgc cgattgtccc gcgaaaaggg tcgaggcgag gatgagcagg ccaagaacgg
    18061 acgcggcctg gcgacgttgc ggagatgaaa aaatgcgcgt gcggcagaag cactcgatgc
    18121 gctgaaaggc ggacaagatc actgcccccg ccgggtcacg actcatttat tttggcagta
    18181 atcgtatatc ttgctgtaaa tttgcaacca agatttttgc tcacccgggg aaaggtgcgc
    18241 catcatcttc ataaggttgc aaatcatttc ctcccggatg cgccggacga gcgccgaccc
    18301 gcgcggggtg atgcaaacca tcactttgcg ccggtcatcc gaggcgtggg aacgcataac
    18361 ataaccaaga ttctccagcc gcgcgacgag tccgctggcg gccgcggtgg tgtgccccat
    18421 tttctgcgcg atggccgaca tcgtcagcac ctccttgagg tcgagataag tgaggaggaa
    18481 gaactgcggg aaagaaacat gtcccggggc gagttccttc gagaggttga ggatgaagca
    18541 gcgctgcatc tccatgatga tgtgcgcgag acgctcggca tcgccgcgca attcatcagt
    18601 cgcggtggtc aaataagcgg tggctgctcc catctttggc acgcctaaac tatgagacat
    18661 gactggagtg tcaaccggtg ccagccttcc tcgcgctcgt gctcctcatc gtgctcgtaa
    18721 tcgaacggga tcgaggggtc gcccgcaggc cctcaccgtg cgcggccggg tggcggcgcg
    18781 cgatggaaaa tttgtcggcg agatcggccg tgggaaattc ctccggcgcg aaccgaatca
    18841 tttgagtcgt taggcgcagg cgcccctatt cgtcggcgcc gattggcgaa ccgtcaggaa
    18901 tacggggcgg cattggaatt tcgagcgctt tgggatcgcg gtggaattga tcgatctgcg
    18961 caagcagata agcattctgc gccatctccg ccggatcgtt cgcggagcgt gccttccatt
    19021 tcgcctccaa gtccgcaatc cgccgcatgg cataggcgtt aacctgcggc tcggtatcgc
    19081 gctcgagcgc gagtggcagg agatgattga ggaactgctt ttcgacggcg cgcgcgattt
    19141 ccttttgcgg acccgtctgc tgggcggtgc gttcgccggc tagcagaatg gcgtcgaaaa
    19201 gttcgttcaa cgatagcccg tgcacggccg ccgcgtcctg cgccacgatc cgtgaaagcc
    19261 gctgcgggtt gagcagcaga tcgagttcgg cgttgatcca actctccgcc gccgccaacg
    19321 gatcgaagac caaaccagtg tgcgaatcga acgattcgcg atcgcgcgga taacccggtg
    19381 gccgcggcgg caggagcgcg accagcttcg ccggcatctc gagaaactcg ggccgcaaag
    19441 tttgcagcac agccgtcagc gcggcgcgtt gttgctccgg cgggagcggt tcgttagtcg
    19501 tctgcccgtc gccgcgggtg gcgtaggtgt aattgacgcc gccgatcaac ttcgtggcgg
    19561 cttcagcctg gaaacgatgc atcaggtaga ccggcacgag cacgttctcg agcgtcgcca
    19621 gcggtgcgcc gggcggaata tttttttcgc tcaggcgatt caaggccagc gcgcgcacct
    19681 tgatcagccg attcaattcg tccgtcgccg agtcgccact atcccagaga tgtgcgaagg
    19741 gattggcgct cccgggcggg cgcgcatccg catccgtcag gtaacggaaa cctcgcgcga
    19801 tattctcctg cagaatggag gcgagcccgg cggcctcgtc cgcctcgctc ggaaactcct
    19861 gatagccgta gagaatggtg cgcttgtccc acgcgccaat tccggtcgcg taagcctggc
    19921 tcaagtcgat gttccccttc gagtcgatcg acacgagcgg cggcggataa tccatgaccg
    19981 acgcgcgatt ttgcgtgctg gccgcgaagt tgtgctgcaa gccaagcgta tggccgactt
    20041 cgtgcgcggc caattgccgc aaccgcgcga gcgacaactc gagcaggcgc gggtcggggg
    20101 tcgcgccgtt cgcataggct tccaccaggc cttgcgcgat cagaaaatcc tgtcgcacgc
    20161 gcagcgagcc taacgacacg tgtcccttga tgatctcgcc ggtgcgcgga tcgataatcg
    20221 acgtgccgta ggaccagccg cgggtcgagc ggtgcaccca ttcgatcacg ttat
//
LOCUS       DATMNS010000292         3268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3535912, whole genome shotgun sequence.
ACCESSION   DATMNS010000292 DATMNS010000000
VERSION     DATMNS010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3535912"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VNW28_10475"
     CDS             <1..302
                     /locus_tag="VNW28_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10399.1"
                     /translation="FQYAAYCALWQPSMRAEDVPFLEHWADLWYREMSATFLASYLRT
                     AAGATFLPQKGSDLQVLLETYLLDKAVYEVGYELNHRPDWVVIPIRGIKHILQLA"
     gene            408..695
                     /locus_tag="VNW28_10480"
     CDS             408..695
                     /locus_tag="VNW28_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10400.1"
                     /translation="MDEAIADKIEAQPELLEIAQTNIARWLAQNHSAPHRLKQWREII
                     LEAQGSAEGLRRLLAILRDPGEEATHLRSFAPFPGVLSIDERRELLCSHKH"
     gene            806..1225
                     /locus_tag="VNW28_10485"
     CDS             806..1225
                     /locus_tag="VNW28_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10401.1"
                     /translation="METSYDADLLVTPYDAELGKLLHEAIGENSIFHARTGYYADVML
                     PGVEETFPRGWAERLVPLPGYEQARCLDPHDLAAVKMQAGRGKDLALCAELLAAGMLK
                     VELIRERLGAVRMTERMITLTEQRLKRAIEMAKRDGE"
     gene            1296..2033
                     /locus_tag="VNW28_10490"
     CDS             1296..2033
                     /locus_tag="VNW28_10490"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HXA10402.1"
                     /translation="MKPFCFCLVFLLGAIMLSSAAETRFENGPQQTALVELYTSEGCS
                     SCPPAEAWLSRLKGNPDLWKKFVPLAFHVDYWDRLGWRDRFSSSQWTERQRGYASLWQ
                     SDSVYTPAVVVNGSEVRDWTAANVSRPNEKQGGVLSAQTTDGKTFAIEFRPDENGTVW
                     EAHAALLGSGISSKIGAGENSGRNLQHDFVVLALREAQMKSDGGIMRARLTLNATGET
                     GARKAVAVWVTLPARLVPVQATGGWLP"
     gene            2667..>3268
                     /locus_tag="VNW28_10495"
     rRNA            2667..>3268
                     /locus_tag="VNW28_10495"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          685 a          874 c         1039 g          670 t
ORIGIN      
        1 cctttcaata cgccgcttac tgcgcgctct ggcaaccatc gatgcgcgcc gaagatgtgc
       61 ccttcctcga gcattgggcc gatctctggt atcgagagat gagcgcgact tttctcgcga
      121 gctatctgcg gaccgcggcg ggtgcgacct ttctgccgca aaaggggagc gacctgcagg
      181 tactgcttga aacctacctg ctcgacaagg cggtttacga agtgggctac gagttgaatc
      241 accggcccga ttgggtggtg atcccgatcc gcgggatcaa acacatcctg caacttgcct
      301 aacggagaga cgcttcccgc gcgttcaggc gatgccgagc attgcttcgg tcaggcgcgc
      361 cggctagatt ggcgccggtg gaggaaactg gtgccagggc gaagtcgatg gatgaggcca
      421 tcgcggacaa aatcgaggcg cagcctgagc tgctcgagat tgcgcagaca aacatcgcgc
      481 gatggctggc acagaatcat tcggcgccgc atcgcctcaa gcagtggcgg gaaattattc
      541 tggaagctca ggggagcgca gaaggtttgc gccgattact ggcgatcctg cgcgacccag
      601 gggaggaggc gacgcattta cgctcgtttg caccttttcc cggagtctta tcgatcgacg
      661 agagaaggga gctgctctgc agccacaagc actaggtcat atcgcggatg cggcgcgcgc
      721 acttgtccgg ggtgaatcga tcgtcattct cggctcctcc gcattgctgg cttcatttcc
      781 gcagctgggc gatgccgggc aacccttgga aacttcatac gatgccgatc ttctggtcac
      841 cccttacgac gcggagctgg gcaagttgct gcacgaggcc atcggcgaaa acagtatttt
      901 ccatgcccgt acgggttact atgccgacgt catgttgccc ggtgtggagg aaactttccc
      961 gcgcggctgg gccgaacggc tcgttccctt gcctggatac gagcaggcac gctgtctcga
     1021 tccgcacgat ctcgcggcgg tgaaaatgca ggcgggcaga gggaaagacc tcgcactttg
     1081 cgcggagttg ctcgccgcgg gaatgctgaa ggtggaactg atccgggagc ggctgggcgc
     1141 ggtgcggatg acggagcgca tgatcacttt gaccgagcag cgtctgaaaa gagcgatcga
     1201 aatggcgaaa cgtgatgggg aataatctgg cttgcgccga caaatcccga aatttcgcgc
     1261 ggaataacca gccggggtac cggctctgat tttcaatgaa accattttgc ttctgtctcg
     1321 tttttttgtt aggcgcaatc atgctttcat ctgctgccga gacgcgcttt gagaacggcc
     1381 cgcagcaaac cgcgctggtc gagctttaca cctccgaggg ctgcagcagt tgcccgccgg
     1441 cggaggcgtg gctgagccga ttgaaaggca atccggattt gtggaagaag ttcgtcccgc
     1501 tcgcctttca cgttgattac tgggatcgcc tcggctggcg cgatcgtttt tcctccagtc
     1561 aatggacgga gcggcaacgc ggctatgcgt cgctttggca gagcgattcg gtttatacgc
     1621 cggctgtggt cgtgaatggg agcgaagttc gcgactggac ggcggccaac gtttcccggc
     1681 ctaacgaaaa gcaaggcggc gtcctgagcg cgcaaacgac agatgggaaa acctttgcga
     1741 tcgagtttcg gccggacgaa aacggcacag tctgggaagc gcacgccgcg ctcctcggct
     1801 ccggcatttc ctcgaagatc ggcgcgggcg aaaacagtgg ccgcaacttg cagcatgatt
     1861 tcgtcgtgct cgcgctgcgc gaggcgcaaa tgaaatccga cggcggcatc atgcgtgccc
     1921 gcctgaccct gaatgccacg ggtgaaaccg gggcgcgcaa agcggtcgcc gtttgggtca
     1981 ccttgcccgc ccgcctcgtc ccggtgcagg cgacgggcgg ctggctgccc tgagcccggc
     2041 gaccgccttt gttgctcgac accggccgcg gtcgagcgaa ccgcggttgc gggaacggat
     2101 tgcctcgccg gagaggtgaa gatttttggt gattgcgggc gaaaatattt ttgacgagcg
     2161 ccaattgtcg cttagtctac cggttccgcc agcaagcggg cccgcgaccg cgggttgttc
     2221 ttcccggctc tggagcgggc cgacgttcga tctgaaaagc gaacagccgg tctttgccgc
     2281 gacgccgggg tgagcagtcg atctttgaca gtgcaattct ggatttcgtc atcacgacga
     2341 ttcggtttat ttgaaatcca gaaggtagaa gtcagcgaaa attgaattgt gcgttatacg
     2401 tcgagtttct ttgattaaaa atttcgccca gcaccttcag ttgattccaa aattcaggag
     2461 acgaccatcg cttcatcgcg cggtggccag tttcaggaag tgaggagtgg attgaaagtg
     2521 ctgggtgatc tcaattaagt aagatcggac gaacctgccc cggctcacga gccgagagca
     2581 ggcaagaatg gtcagaaata gtcggtcctt tagcgaggac cggcgcaaac tttgtccggt
     2641 gtcagcgatg acgccggctc caaattttta cggagagttt gattctggct cagaacgaac
     2701 gctggcggcg tggataagac atgcaagtcg aacgggactt ttccgagtag caatacctgg
     2761 gaaagtctag tggcgaacgg gtgcgtaaca cgtggggaat ctgccgagaa gtgggggata
     2821 gctcgccgaa aggcgaatta ataccgcata cgatcaggga agacatcttt ccgacatcaa
     2881 agccggggca acctggcgct tcctgaggac cccgcggcct atcagctagt tggtgaggta
     2941 acggcccacc aaggcgatga cgggtagctg gtctgagagg acgaccagtc acactggaac
     3001 tgagacacgg tccagacacc tacgggtggc agcagtcgag aatttttctc aatgggggca
     3061 accctgaagg agcgacgccg cgtggaggat gaaggctttc gggttgtaaa ctcctgtcat
     3121 ttgggaacaa gttgcccacg ttaactgcgt gggcattgat agtaccagaa gaggaaggga
     3181 cggctaactc tgtgccagca gccgcggtaa tacagaggtc ccaagcgttg ttcggattca
     3241 ttgggcgtaa agggtgcgta ggtggcgc
//
LOCUS       DATMNS010000293         7692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3536961, whole genome shotgun sequence.
ACCESSION   DATMNS010000293 DATMNS010000000
VERSION     DATMNS010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7692
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3536961"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(452..1225)
                     /locus_tag="VNW28_10500"
     CDS             complement(452..1225)
                     /locus_tag="VNW28_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HXA10403.1"
                     /translation="MPQGIRLLSTDFDGTLVSHASEPVFDRDCMALIRELQRAGAIWA
                     INTGRSVSLLEEGLEDFAFPLQPDFILTSERDVFRPTQDGWEPFGDWNARCAQAHAEL
                     YQSASSILAEVIDFVSRQTKARVIYEADEPAGLIASSEEEMDRLTKFIDQAKAGESQF
                     HYQRNTIYLRFCHAAYHKGAALEELSRLTEIPRAAIFAAGDHHNDISMLDGRYAAHPA
                     CPANAIAEVKEAVRRAGGWVAEKEHGAGVHEALQHFLTF"
     gene            1310..1594
                     /locus_tag="VNW28_10505"
     CDS             1310..1594
                     /locus_tag="VNW28_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10404.1"
                     /translation="MKSAYELAMERLEKSAPTVRLTDEQKAQIAEIDSTYKARIAEKE
                     LFLRDKIREAGLGGNSQEFEQLEAQLAAEVRRLREDCEEKKAKVRASFQS"
     gene            1831..2793
                     /locus_tag="VNW28_10510"
     CDS             1831..2793
                     /locus_tag="VNW28_10510"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXA10405.1"
                     /translation="MFALQPELKVFSGSANRELAQRICKFIGVPLGQATISSFPDGET
                     YIKIDENIRGRDVFIVQPTCPPTNEHLMELLIMVDAARRASADRITAVIPFFGYARQD
                     RKDQPRVPITAKLVANLLCAAGVSRVLTMDLHAQQVQGFFDIPVDHLYSLPVLIPYLR
                     GKLKRKTAVVSPDVGGLKMASAYSQALGASLAIVAKQRKSATETEALYLIGEVEGCDV
                     LLVDDLTETAGTLTSAATLLKKNGALDIYAGVAHAVLADVALERLQKSQIKELITTNS
                     VPVRTVEGFTTTVLCVSELLGESIKRIHNDESVSSLFKIDNGAQ"
     gene            2806..3519
                     /locus_tag="VNW28_10515"
     CDS             2806..3519
                     /locus_tag="VNW28_10515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HXA10406.1"
                     /translation="MAKQVKLSATRRTGTGRSAVRKIKAQGAVPAIIYGGKNKAEALQ
                     VSKRDISLMLSHASGENILVELEIDGEKSGRLALVQEVQHAPLGGDILHIDFHAVSMD
                     EMIEADVPLEPTGIPEGVKTFGGLLEQNIRSLEIECLPRDLPDVITVDVSHLNIGDSI
                     HVRALPLPSGVTTRVPADLTAFSVLAPTVEEEPVVAAEEAPTAPEVITAKKEPAEGGA
                     APAAPSKGAAPAKEKEAKK"
     gene            3546..4127
                     /gene="pth"
                     /locus_tag="VNW28_10520"
     CDS             3546..4127
                     /gene="pth"
                     /locus_tag="VNW28_10520"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HXA10407.1"
                     /translation="MNEAVAPIRLIAGLGNPGREYQRTRHNVGFMVLDRLAADAKLPW
                     DYSEKWGAAWAKSNALLVKPATYMNRSGQPLSAMANFYKIAAAEILVVLDDFALPLGR
                     LRLRSQGSSGGHNGLESIFEHFGTEEIPRLRIGIGGAPAEGAVDYVLGRFFDEEKPVL
                     DEVIVRATEAVKCAVDKGLFAAMNVFNKPPEEL"
     gene            4124..4456
                     /locus_tag="VNW28_10525"
     CDS             4124..4456
                     /locus_tag="VNW28_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HXA10408.1"
                     /translation="MKNRYEALLVLNTQGKDDAVKDIVDRLESEFQKEGAQIEQVQKM
                     DKRPFSYVAGDLDSGFYVNFVFSADSQLITKLRSKFKLDPEVYRQHYQRLSDKKALPA
                     KKRVAVEK"
     gene            4475..4954
                     /gene="ssb"
                     /locus_tag="VNW28_10530"
     CDS             4475..4954
                     /gene="ssb"
                     /locus_tag="VNW28_10530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXA10409.1"
                     /translation="MASYNKVFLLGNLTRDPEVRYTPKGSAVADLGIAVNRQYTLDTG
                     EKREEVTFVDVTFWGRTAEVAGEYLKKGRSVFIEGRLQLDTWDDKQSGQKRSKLKVIG
                     EMMQMLGGRPGGGSGGGGGEEGGGAEEAPRARSGGSARSAAPPKGAPAEPDDDEIPF"
     gene            5012..6112
                     /gene="dprA"
                     /locus_tag="VNW28_10535"
     CDS             5012..6112
                     /gene="dprA"
                     /locus_tag="VNW28_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXA10410.1"
                     /translation="MNSTEACIALNMVPQMGPVRLRKLLEVFATPERILAAKRNELRA
                     IDGIGHEVADQIAHWESAVDLSAELIRILDFGAEVITAQSPTYPRQLREIHAPPIVLY
                     VWGELTERDQHAIGVIGSRRTSHYGAECAKKLSYQLAYAGLTIISGLARGIDTAAHQG
                     ALAAKGRTIAVIGSGLMKLYPPENAALAEKIRSGNGAILSEFSMAVEPDRQTFPMRNR
                     IISGWSHGILVVEAGINSGALITAAQALEQGRSVYAVPGHINAPTAHGSNRLIQQGAK
                     LVMDASDILDDLQILLPEKQKLPEVSARALPELSADERRVYDAINPTETPIDRIATTC
                     ELPSATVSSVLLRLELKRLVKQLPGKYFVKLS"
     gene            6177..>7692
                     /locus_tag="VNW28_10540"
     CDS             6177..>7692
                     /locus_tag="VNW28_10540"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HXA10411.1"
                     /translation="MAKTLIIAEKPSVAQDLARALGKIPKKGDHFENDEYVISSAVGH
                     VVELEMPEDIDKKKYGFWRLETLPIIPERFGLKPIADSKDRYNQLKKLLHRKDIEMVI
                     NACDAGREGELIFQNLYQLSKSKLPVKRAWMQTMTAEGIREAFRTLRAGEQMAGLADA
                     ARSRSESDWLIGINGTRAITKRMFGSRGGNVASVGRVQTPTLAIVFNRELEIRDFKPQ
                     DFWRVTAKFEIAKGTYEGVYQRPNFKKDEKNEHDRIDRVWEKAIAEAALAACQGQPLA
                     KVSEEKKSSSQASPRLYDLTTLQREANNRFGLSARRTLQIAQALYERHKVITYPRTDS
                     RALPEDYIATCKQTLGKLPGDLGKHGQTVLVNGWVRPNKRIFNNAQISDHFAIIPTGT
                     ASTHLDEMESKVYDMIARRFVAAFFPAAEFDVTTRISTVTGKHDFKTEGKVLTAPGWL
                     AVYGKTTVDDSGDAKALPALTSADNSQAKTISAELHAETTKPPPRYTEATLLSAM"
BASE COUNT         1660 a         2298 c         2337 g         1397 t
ORIGIN      
        1 cacgcctttc acgacatgga cacgaccgtt agctggcccc cgttgggcga ggtcctcgcg
       61 gaggacgcgc gcctcaggat gtagcgagag cgacctcaca ctcaccgtcg gtccaaaggg
      121 atttcagatt gcccggctac gaatgcggcg acggcaacga ttttactctg acggtcgtgc
      181 cggagttgta gccgcttcgc tatgcgaagc gcgggagaga ggtcggaacc ctggcttgcc
      241 accacgccca atagcccacg ccgcccacag ggcggcggct acaacgctga aggcggcgat
      301 gggaatgatc tcacgctaat ggtggtgtcg tagcgatttg agattgtagg gcaagcgctt
      361 cgcttgcctt gcttgaccca gggcagccgg cgcgcccgcc ctacaaatcc ctccgctttg
      421 ccgtgcgcgg acgacagcgg ctgccgctgc atcagaaagt caggaaatgc tgcagcgctt
      481 cgtgcactcc cgcgccgtgt tccttctccg ccacccaccc gccagctcgg cggacggcct
      541 ccttgacctc ggcgatggcg ttggccggac acgccgggtg cgcggcataa cggccgtcga
      601 gcatcgagat gtcgttgtga tgatcgcccg cggcgaaaat ggcggcgcgc gggatctcgg
      661 tcaggcggga aagctcttcc agggcggcgc ctttgtgata agcggcgtgg caaaagcgca
      721 gatagatagt gttccgctgg taatgaaact gcgactcccc ggccttggcc tggtcgatga
      781 atttcgtgag gcgatccatc tcttcctcgc tcgaggcgat caggccggcg ggctcatccg
      841 cctcgtagat gacacgtgcc ttggtttggc ggctgacgaa atcgatcacc tccgctagga
      901 tggaggaggc ggattggtaa agctcagcgt gagcttgtgc gcagcgcgca ttccaatcgc
      961 caaaaggttc ccacccatcc tgtgtcggac gaaaaacatc acgttcactc gtgaggataa
     1021 aatccggctg gagcgggaag gcaaaatcct ccaggccctc ctcgagcagg ctgacggagc
     1081 ggccggtgtt gatggcccag atcgcgccag cccgttgcag ctcgcggatc agcgccatgc
     1141 agtcgcggtc gaagaccggt tcgctcgcgt gcgaaacgag cgtgccatcg aagtcggtgc
     1201 tgaggagtcg gatcccttgc ggcatttgga aggggaactt aggaacggat ttcgcctgcg
     1261 caatgcatcg attacgatta cgatcaggaa caggaaaggg agcggaaaga tgaaatcagc
     1321 gtatgaactt gcgatggagc gcctcgagaa aagcgcgccg acggtcaggc tgacggacga
     1381 gcaaaaggcg caaatcgccg aaatcgactc gacttacaaa gcgcggatcg cggagaagga
     1441 gctctttttg cgggacaaaa tccgcgaggc cggtttaggc gggaactccc aggagtttga
     1501 acaactggag gcccaacttg cggcggaggt ccggcgcctg cgcgaagatt gcgaggaaaa
     1561 aaaggccaaa gtccgtgcct cctttcagag ctgaagcggc tggacggtgc gaaagtttga
     1621 ttgacgccgt cgggcagttt tgatattcct tgaatcatca gccggagcgt gtcgcctaag
     1681 agctccgatg aagagtttgt ctggtgtttg gcggaggcga cggaggtgtt ttccgtcgcc
     1741 tccgtttttt tgtggcagga atcttccggg cgcgttcgga tttctttgcc aactctgaag
     1801 aatcgcgcct aagctaccgg ccctcgacag atgtttgctc tccaacctga gcttaaagtt
     1861 ttctccggtt cagccaatcg cgaactggcg caacgcattt gcaaattcat cggggtcccg
     1921 cttggccagg cgacgatcag ctcgttcccc gacggcgaga cctacatcaa gatcgacgaa
     1981 aacatccgcg gccgtgacgt cttcatcgtc cagccgacct gcccgccgac caacgaacac
     2041 ttgatggagt tgctcatcat ggtagacgcg gcgcggcggg cgagcgccga tcgcatcacg
     2101 gcggtcattc cgttttttgg ttacgcgcgg caggaccgca aggatcagcc gcgtgtcccg
     2161 atcacggcga aactggtggc caatttactc tgtgccgcag gtgtgagtcg ggtattgacg
     2221 atggatttgc acgcgcagca ggtgcagggc tttttcgata ttccggtgga ccatttgtat
     2281 tcgctgccag tcttgattcc ttacctgcgc ggaaagctga agcgcaagac cgccgtcgtg
     2341 tcgcccgatg tgggcggctt gaaaatggcg agtgcctatt cgcaggcgct cggcgccagc
     2401 ctcgcgatcg tcgccaagca gcgcaagagc gcgaccgaaa ccgaagcgct ctatttgatc
     2461 ggtgaagtgg agggctgtga tgtgctcctg gtcgatgatc tgaccgagac cgcggggacg
     2521 ctgacctccg ccgcaacttt gctgaaaaag aacggcgcgc tcgacatcta cgccggggtc
     2581 gcccacgcgg tcctggcgga tgtcgcgctc gagcggttgc aaaaatccca aattaaggaa
     2641 ttaataacga ccaacagcgt gcccgttcgc accgtggaag gctttacgac cacggtgctt
     2701 tgcgtctctg agctgctcgg ggaaagcatc aagcggattc ataatgacga gtctgtttca
     2761 tcgctcttca agatcgataa cggcgcgcaa tagcaaactg ataccatggc gaaacaagta
     2821 aaactttcag cgacccgacg caccggcact gggcgatccg cagtccgcaa aatcaaggcg
     2881 caaggcgccg tgccggcgat catatacggc ggcaagaaca aggccgaggc gctccaggtc
     2941 tcgaagcgcg acatctcgct gatgctcagt cacgcttccg gcgaaaacat tcttgtcgaa
     3001 ctcgagatcg acggtgaaaa atccggccgc ctcgcgctcg tgcaggaagt gcagcatgcc
     3061 cctctcggcg gcgacatttt gcatatcgat tttcacgccg tctcgatgga cgaaatgatc
     3121 gaggccgacg ttcctctcga gccgaccgga atccccgagg gcgtcaagac cttcggcggt
     3181 ttgctcgagc aaaacattcg ctcgctcgag atcgaatgtc tgccgcgcga tctgcccgac
     3241 gtgatcacgg tcgacgtttc gcatctcaat atcggcgact cgattcacgt gcgcgcgctt
     3301 ccgctcccgt ccggtgtgac cacgcgtgtg cccgcggatc tgaccgcgtt ctcggtcctc
     3361 gcgccaacgg tggaggaaga gccagtggtg gcggcggagg aagctccgac ggcgcccgaa
     3421 gtgatcacgg cgaagaaaga accggcggaa ggtggtgccg cgccggccgc cccttcgaag
     3481 ggtgcagctc ccgcgaagga gaaggaagcg aagaagtaac ttccgcaccc ggcgccggaa
     3541 cggacgtgaa cgaagcagtc gcgccgattc gcttgattgc gggtctggga aatcccgggc
     3601 gcgagtatca gcgaacgcgg cacaacgtcg gcttcatggt gctcgaccgg ctggccgccg
     3661 atgcaaagct tccgtgggat tactcggaaa aatggggcgc agcctgggcg aaaagcaacg
     3721 cgctgttggt gaaaccggcc acttatatga atcgcagcgg tcagccgctc tccgccatgg
     3781 caaatttcta caagatcgcg gcggcggaaa tcctcgtcgt gctcgacgac ttcgcgttgc
     3841 cgcttggccg gttgcgcctg cgttcgcagg gcagctcggg cggacacaac ggactcgagt
     3901 cgattttcga acattttggg actgaggaaa tcccgcgctt gcggatcggg atcggcggcg
     3961 cgcctgccga gggcgcggtc gattacgtgc tcggtcgctt ttttgacgaa gaaaaacctg
     4021 tcctcgacga agtgatcgtg cgtgcgacgg aagcggtgaa gtgcgcggtc gacaaaggat
     4081 tgtttgctgc gatgaatgtt ttcaacaaac cacctgaaga attatgaaga atcgttatga
     4141 agcgttgctc gtgctcaaca cgcagggcaa ggatgatgcc gtgaaggata tcgtggatcg
     4201 cctcgaatcc gaatttcaaa aagagggcgc gcagatcgaa caggtccaaa agatggacaa
     4261 gcggccgttc tcctacgtcg cgggcgatct cgactccggc ttttatgtca attttgtctt
     4321 cagcgcggac tcgcagttga tcacgaaatt gcgttccaag ttcaagctcg acccggaagt
     4381 ttaccggcag cactaccagc gcctgagcga caaaaaagcg ctgccggcga aaaagagagt
     4441 cgcggtcgag aaatagatcc gctagaaaag tcgcatggcc agttacaata aagttttcct
     4501 cctcggcaat ctgactcgcg atccggaagt tcgttatacg cccaaaggca gcgcggtcgc
     4561 ggatcttggc attgcggtca accggcaata cacgctcgac acgggcgaga aacgcgaaga
     4621 agtcaccttc gtcgacgtga ccttctgggg tcgcaccgcc gaagtggccg gcgaatattt
     4681 gaagaaggga cggtcggttt tcattgaagg ccggctgcaa ctcgatacct gggatgacaa
     4741 gcaatcgggc cagaagcgca gcaaactcaa agtgatcggc gagatgatgc agatgctggg
     4801 cggccgtccc ggcggcggca gcggtggtgg cgggggcgag gaaggcggcg gcgccgagga
     4861 agcgccgcgc gcgcggtccg gaggcagtgc gcgcagtgca gcgccgccca agggcgcgcc
     4921 cgccgagccg gacgacgacg agattccatt ctagcttttt tccgcgcgag ctcgcgccta
     4981 acgacaaatc tccgaccttc ggttgccatc catgaacagc accgaagcct gcatcgcgct
     5041 caacatggtt ccgcagatgg ggccggtgcg cctgcgcaag ttgctcgagg tcttcgcaac
     5101 gccggaacgc attctcgccg cgaaaagaaa cgaactccgt gcgatcgacg gcatcgggca
     5161 tgaggtcgcg gaccaaatcg cgcattggga aagcgcggtc gatctttccg ccgaactcat
     5221 acgcattcta gatttcggcg ccgaggtgat caccgcgcag tcgccgacgt atccgcgcca
     5281 gctccgcgag attcacgcgc cgccgattgt gctctatgtc tggggcgaac tgacggaacg
     5341 cgatcagcac gcgattggcg tgatcgggtc gcgccgcacg agccattacg gcgcggaatg
     5401 cgcgaagaaa ctctcctatc aactcgccta tgcgggcctc accatcatca gcgggctcgc
     5461 acgcgggatc gacaccgcgg cgcatcaggg tgcgctggcc gcgaaaggcc ggacgatcgc
     5521 cgtgatcggt tccgggctga tgaaactcta tccgccggaa aatgcggcgc tcgcggagaa
     5581 aattcgcagc ggaaatggcg cgatcctctc cgagttctcg atggcggtcg agccggaccg
     5641 ccagaccttc ccgatgcgca accgcatcat cagcggctgg agtcacggga ttctggtcgt
     5701 cgaagccggg atcaacagcg gggccctcat caccgccgca caggcgctcg agcagggccg
     5761 gtccgtttac gccgtgcccg gccacatcaa cgccccgacc gcacacggct cgaatcggct
     5821 catccagcaa ggcgcaaaac tcgtgatgga cgcgagcgac atcctcgacg atctgcaaat
     5881 tctgctcccg gaaaaacaaa aactgccgga agtttcggcg cgggctctgc cggagctgag
     5941 cgcagacgaa cggcgcgttt acgatgcgat caatccgacc gaaacgccga tcgatcggat
     6001 cgccactacc tgcgagcttc ccagtgccac cgtctcctcc gtactcctcc ggctggaact
     6061 gaaacgcctc gtcaaacagc tgccgggaaa atatttcgtc aagctctcgt agcgcgctcg
     6121 cttcgtgctg ctctcacttg caaacctcgc caacgaggtc tacttgccat cccttcatgg
     6181 ccaaaaccct catcatcgcc gaaaagccca gcgtcgcgca ggacctcgcc cgcgctcttg
     6241 gcaagatccc caagaaaggc gaccacttcg agaacgacga atacgtcatc tcgtcggctg
     6301 tgggccacgt cgtcgagctg gaaatgcccg aggacatcga caaaaagaaa tacggttttt
     6361 ggcgcctcga gacactaccg attatcccgg aaagattcgg acttaagccg attgccgatt
     6421 cgaaagaccg ctacaaccag ctcaagaaac ttctccatcg caaagacatc gagatggtga
     6481 tcaacgcctg cgacgccgga cgcgaaggcg agttgatctt ccaaaatctt taccagcttt
     6541 ccaaatcaaa gctcccggtg aagcgcgcct ggatgcagac gatgaccgcg gaaggaatcc
     6601 gggaagcgtt tcgcaccttg cgcgcaggtg agcagatggc cggcctcgcc gacgccgccc
     6661 gctcccgcag cgaaagcgac tggctgatcg gcatcaacgg cacgcgcgct atcacaaaac
     6721 ggatgtttgg ctcgcgcggc ggcaacgttg cttcggtcgg acgcgtgcaa acaccgacgc
     6781 tcgcgatcgt cttcaaccgc gagctggaga ttcgcgattt caagccacag gacttttggc
     6841 gcgtgaccgc gaaattcgaa atcgcgaaag gcacctacga aggcgtttat cagcgaccga
     6901 atttcaagaa ggacgagaag aacgagcacg accggatcga tcgcgtctgg gaaaaagcga
     6961 tcgcggaagc cgcgctcgcg gcctgccagg gccagccgct ggcaaaagtc agcgaggaaa
     7021 agaagtcctc gagccaggct tcgccgcggc tctatgacct gacgactttg caacgcgaag
     7081 cgaacaatcg ctttggcctc tcggctcgcc gcaccctgca gatcgcgcag gcgctctacg
     7141 agcggcacaa ggtcatcact tatccgcgca ccgattcgcg cgcgctgccg gaggattaca
     7201 tcgccacctg caagcaaacg ttaggcaaac ttcccggcga cctcggaaaa catggccaga
     7261 ccgtgctcgt gaacggctgg gtgcgtccga acaagcgcat cttcaacaac gcacagatct
     7321 cggatcattt cgcgatcatc ccgaccggca ccgcgtcgac tcatctcgat gaaatggaaa
     7381 gcaaggttta cgacatgatc gcgcgccgtt tcgtcgcggc gttttttccg gcggccgaat
     7441 ttgatgtcac gacccgcatc agtacggtga cgggcaagca cgatttcaag accgaaggca
     7501 aagtcctgac cgcgcccggg tggctcgcgg tctacgggaa gacgacggtc gatgattcgg
     7561 gagacgccaa ggcgctgccc gccctcacga gcgcagataa ctcgcaggcc aaaaccattt
     7621 ccgccgaact gcacgccgaa acgaccaagc cgccgccgcg ttacaccgaa gccacgctgc
     7681 tctccgcgat gg
//
LOCUS       DATMNS010000294         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3537270, whole genome shotgun sequence.
ACCESSION   DATMNS010000294 DATMNS010000000
VERSION     DATMNS010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3537270"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..1104)
                     /locus_tag="VNW28_10545"
     CDS             complement(397..1104)
                     /locus_tag="VNW28_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10412.1"
                     /translation="MDASLGETAAHLELKRLALLWAQENGYPIAALEVSLPQCRYRAD
                     VAAYRPQKNGALGQTAVFECKQSAPDLRRDDCRSPQTLARLESAYRRRQVLEKHLRIH
                     YPTLRTGESLFPEWDAHDFAMIEHRGYAQVTRELAALQNQLRDGRKFEKLSRYASANL
                     FFLVAPNELLREPEAPLGWGLLVEKAGALTLQRKPVWHDSPETARLRVLQKIAAAGTR
                     QINRRLEVDPARPAFGR"
     gene            complement(1089..2075)
                     /gene="hemB"
                     /locus_tag="VNW28_10550"
     CDS             complement(1089..2075)
                     /gene="hemB"
                     /locus_tag="VNW28_10550"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HXA10413.1"
                     /translation="MRTLVHRPRRLRRTAALRNLVRETNLSPHDFILPLFVSEKISAR
                     RAIGSMPGVAQFAPDEIAGEAARANAAGLQAVLLFGIPTEKDETASGAHAEDGVVQQA
                     IRAIKAECPELLVITDVCLCEYMSHGHCGVTRIDGDHFHVLNDESVELLVKTALSHAA
                     AGADLVAPSDMMDGRVGAIRGALDAAGYDQTGIISYAAKFASALYGPFREAAESPPQF
                     GDRRTYQMDPANAAEALREVALDVAEGADIVLVKPALPYLDILWRVRERFGLTTAVYH
                     VSGEYAMVKAAAEKGWLDERQAVLEIMTGLKRAGADLIITYWAKELAEWTRR"
BASE COUNT          407 a          667 c          746 g          422 t
ORIGIN      
        1 ccgcctccgc ggcgctgctg atattcaaat gtgatgcgcg ggagcgaacc gccgcgccgg
       61 gtggggtcgt cataacggga caaccacttt accgattcaa tctggtcatt gggtttcatt
      121 ttgttttcct tgcgaaatgg gaaattccga aaagagcgaa cgcccgtgaa gggcattcga
      181 gtttccattc aggcccaccc agtttggtgg cccaaaaaga acgcgccatg aggcgaagcc
      241 gatccccggc cgcgtaccgc cgcgcatgaa tcgatagcat ttcgcgaaga atctaaaacg
      301 gatttttcgc gggtccagag aacccggcgt aacagactgg taaaatgcag gttacggcaa
      361 tcagagaact gcccatccct ccggagcggg cccgtttcag cgcccgaacg cgggacgcgc
      421 cgggtcaacc tccagccggc gattgatctg ccgggtgcca gcggcggcga ttttttgcag
      481 gacgcgcaaa cgcgccgttt cggggctgtc gtgccagacg ggtttgcgct gcagggtgag
      541 cgcgcccgct ttttccacga gcaggcccca gcccaacggt gcctccggct cgcgcaggag
      601 ttcgttaggc gcgacgagga agaagagatt agcggaggcg tagcggctga gtttctcgaa
      661 tttccgtccg tcgcggagct ggttttgcag agcggctagc tcgcgagtga cctgggcgta
      721 gccacggtgt tcgatcatgg cgaaatcgtg cgcatcccat tcgggaaaaa gagattcgcc
      781 tgtgcgcaga gtagggtaat ggatgcgcaa atgcttctcg agcacctgcc ggcggcggta
      841 agctgactca aggcgggcca gggtttgcgg gctgcggcag tcgtcgcgcc ggaggtcggg
      901 cgcggattgt ttgcattcga agacggcggt ctgaccgagc gcgccatttt tttgcggacg
      961 gtaggcggcg acgtcagcgc ggtaacggca ttgcgggagg gagacttcga gcgccgcgat
     1021 cgggtaaccg ttctcctgcg cccagaggag cgcgaggcgt tttaattcga gatgagcggc
     1081 cgtttcgcct aacgacgcgt ccattcagcc aactccttcg cccaataggt gatgatgaga
     1141 tcggccccgg cgcgcttcag gccggtcatg atttcgagca cggcctggcg ttcatcgagc
     1201 caaccctttt ccgccgctgc tttgaccatt gcgtattcac cgctcacgtg ataaaccgcg
     1261 gtcgttaggc caaaacgttc gcgcactcgc cagaggatgt cgagataggg cagcgccggt
     1321 ttcacgagga cgatgtccgc cccttcggcg acgtcgaggg cgacttcgcg gagcgcttcc
     1381 gcggcgttcg ccggatccat ttggtaggta cgacggtccc cgaattgcgg cgggctttcc
     1441 gccgcctcgc gaaacggacc gtaaagtgcc gacgcgaatt tcgcggcgta acttatgatc
     1501 ccggtttgat cgtaaccggc cgcgtcgagc gccccgcgga tcgcgccgac gcggccgtcc
     1561 atcatgtcgc tcggcgcgac gagatcggcg ccggccgcgg cgtgggaaag agctgtcttt
     1621 accaggagct cgacgctctc gtcgttcagg acgtggaaat ggtcgccatc gatgcgcgtg
     1681 acgccgcaat gcccgtggct catgtattcg cagagacaga cgtcggtgat gacgagcaat
     1741 tcgggacatt cggccttgat cgcgcggatc gcctgctgga cgacgccatc ttccgcatgg
     1801 gcgccggaag cggtttcgtc cttttcggtc gggatgccga agagcaggac cgcctgcagg
     1861 ccggcggcgt tcgcgcgcgc cgcttcgccg gcgatttcgt ccggcgcgaa ttgcgcgacg
     1921 cccggcatgc tcccgatcgc acggcgggcg ctgattttct cgctcacgaa aagcgggagg
     1981 atgaaatcat gcgggctgag gttggtctcg cggacgagat tgcggagggc cgcggtgcgc
     2041 cgcaggcgcc gcgggcgatg aaccaatgtc ctcacggccc gaggctacgc gcaccgattg
     2101 gacgtcgcaa gcatcggcgc gcgggggagc gatcagctca tgaattcggc caacggctcc
     2161 atcggcttga tgtggctgca gaaaatcttg aacgcggcca ggatcgggac cgcaaggacg
     2221 atgccgacga tgccccacat cc
//
LOCUS       DATMNS010000295         4834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3175890, whole genome shotgun sequence.
ACCESSION   DATMNS010000295 DATMNS010000000
VERSION     DATMNS010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3175890"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VNW28_10555"
     CDS             <1..429
                     /locus_tag="VNW28_10555"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HXA10414.1"
                     /translation="AATIERRKIDPQALREGFVRLSGRFDFVAVEGVGGWMVPITPDY
                     FSNDLAADLRLPVIVVAANRLGCLNHVLLTVRSIEGAGLKCAGVVLNNLGESSEVATA
                     TNAEILQRCLPLPILPCFNRDSAEIAPSLCRMMPEIRRLL"
     gene            589..1041
                     /locus_tag="VNW28_10560"
     CDS             589..1041
                     /locus_tag="VNW28_10560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXA10415.1"
                     /translation="MSQSLDECKNRLASIHSYRRQFVMVTKATMTSSKTLDLSFRGPL
                     GFKGETVVAAIESDAPDQFAFESWGGNIDLMGLVEFEEVRPDCTEVTLAVHYEIKNKL
                     YAWLDRKFGFVDAFLTTELRSLRAHFEGIAAPMRERAAAYGMLEPVSA"
     gene            complement(1097..2269)
                     /gene="tyrS"
                     /locus_tag="VNW28_10565"
     CDS             complement(1097..2269)
                     /gene="tyrS"
                     /locus_tag="VNW28_10565"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXA10416.1"
                     /translation="MDPAESFALLKRGAAQIISEAELRQKLSLGRPLRVKLGVDPTSA
                     DLHLGHTLLLRKLRQFQDLGHEAILIIGDFTAMIGDPSGRSVTRPQLTHEEVLANAES
                     YRTQAFKVLDPERTRTVFNGDWFKAMSFAEVIRLNSRVTLQQMLQREDFRQRIDQQQP
                     IRAHEIQYPIMQGWDSVQVQADVELGGTDQLFNILVGRDLQKEEGQPQQVVLVLPILE
                     GLDGVQKMSKSLRNYVGVTDSPNDMFGKLMSISDELMARYYSLLLGRAQPVELHPMEA
                     KKLLAREIVATYHSPTEGQHAQAEWERRFSARQLQQADLPAFAPPAAERNIVSLVVAA
                     YAGAFGLTKSRGEVRRLVEQGSVQWRGEKICDLQAEIALRSGDVLRLDKTRAVRVK"
     gene            complement(2333..2848)
                     /locus_tag="VNW28_10570"
     CDS             complement(2333..2848)
                     /locus_tag="VNW28_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10417.1"
                     /translation="MRAFFLFLTLTTGFASAQVPKQEPKFADRLLRPDMSLANPAQGR
                     KFTAGGGTPADKKFVTKSFYSGDERATRSFWGSKAFSSKDFATGKFYRAEATANARTK
                     AQLAYANARFPTRESALVRSAAAEGKVVRMRAYADCRPFLGEGTRQEILSREDKGLTI
                     DEVRELLNKSR"
     gene            complement(2939..3589)
                     /locus_tag="VNW28_10575"
     CDS             complement(2939..3589)
                     /locus_tag="VNW28_10575"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HXA10418.1"
                     /translation="MIRKLPSVLLASCLALSAASAATEQEVVNQSANILHDFHQMPEH
                     QIPRRILREAHGLAIIRVLKAGFVVSGNGGEGVVVARDANGWSGPSFIGLGGAGWGLQ
                     IGAQLTDFIFVLNNDAAVRAFSRDGNLKLGADVSAAAGPVGRDLQAGVTPTAAVYTYS
                     RSAGLFAGISLEGAIIGTQKTANERYYGRPVGARAILSGSVAPPPGAQRLVNLLDR"
     gene            complement(3586..3747)
                     /locus_tag="VNW28_10580"
     CDS             complement(3586..3747)
                     /locus_tag="VNW28_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10419.1"
                     /translation="MDLSYSLENEASAAKEFGAARKILFDPVVIAPFSKNWPTNNTSA
                     LGRGDEIAL"
     gene            3748..4236
                     /gene="tadA"
                     /locus_tag="VNW28_10585"
     CDS             3748..4236
                     /gene="tadA"
                     /locus_tag="VNW28_10585"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HXA10420.1"
                     /translation="METIIDLQSDAYFMSEALRMAARAYEAEEVPIGAVVARQGKIIA
                     RACNQVELLKDATAHAEMLALTQAEAALGDWRLTDCDLFVTKEPCPMCAGALVHVRMR
                     RVVFGCADPRGGAAGGLLNLLQNPSLNHQCDVTAGVLRDECAELLRSFFRAKRSREAE
                     AQ"
     gene            complement(4244..>4834)
                     /locus_tag="VNW28_10590"
     CDS             complement(4244..>4834)
                     /locus_tag="VNW28_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10421.1"
                     /translation="YDSAGVLVESNDNWTVIPGIPNPLQPANSSESLLTAILPAGSYT
                     AILQGAGNDTGIALVEAYDLTPANSTVTNISTRGDIVTPNDVLIGGFMVGGTDPTSVI
                     VRALGPSLGALGVPTPLPDPYLELHDSNGTLLDANDNWRDTQEQQIIATTIPPTNNLE
                     SAIVETLSPGSYTAVVRDANFKTGIALVEVYDLEPQ"
BASE COUNT          917 a         1406 c         1538 g          973 t
ORIGIN      
        1 gccgcgacaa tcgagcggag gaaaatcgat cctcaggcgt tgcgcgaagg attcgttagg
       61 ctgagcgggc gttttgactt tgtcgcggtc gagggggtgg ggggctggat ggttccgatc
      121 acgccggatt atttcagcaa tgatctcgcc gccgacttgc ggttgccggt gatcgtggtc
      181 gcagccaacc ggctcggctg tctgaaccac gtcctgctca cggtccgaag catcgaggga
      241 gcaggcttga aatgcgccgg cgtggtcttg aacaacctcg gggaatcgtc cgaggttgcg
      301 accgcgacga acgcagaaat cctgcaacgc tgcctgccgt tgccgattct cccatgtttc
      361 aaccgtgaca gtgccgagat cgctccgtcg ttgtgccgga tgatgccgga aatcaggcgg
      421 ttactgtaat cggacgggcg ttggtttggt gacagttgtg taacagaaat gaacacaaac
      481 gagggggttc agagccgtct tccagaggga gagcgagtct cggcacatgc attgctaaat
      541 gtattgcaca accacatcaa acctatgttg ctacaaaaaa ccctgcacat gagccagtcg
      601 ctggatgagt gcaaaaaccg cctcgccagc atccattcgt atcgccgcca gttcgtgatg
      661 gtgaccaaag cgacgatgac ctcatccaag acgctcgatc tcagtttccg tggtccgctt
      721 ggattcaagg gagagacggt ggtggcggcg atcgagagcg acgcgccgga ccagttcgcc
      781 tttgaatcgt ggggcggcaa catcgacctg atgggcctgg tcgagtttga ggaagtgaga
      841 ccggattgca ccgaggtcac gctcgcggtg cattatgaaa tcaaaaacaa gctctacgcg
      901 tggctcgacc ggaagtttgg gttcgtcgat gcgtttctga ccacggagct gcggagcctg
      961 cgggcgcatt tcgaagggat cgctgccccg atgagggagc gggcggcggc ctacggaatg
     1021 ctcgagccgg tctcggccta acgaagagaa gtttctccat gaagggtgcg gaaccgcgag
     1081 gccggaccgc gctcttttac tttacccgaa cggcgcgggt cttatcgagt cgcaggacgt
     1141 ccccggatcg aagcgcgatc tcggcctgca gatcgcaaat tttctcgccg cgccattgca
     1201 cgcttccctg ctcgaccagg cgccgcactt cgccgcgcga tttcgttagg ccaaaagccc
     1261 cggcgtaggc tgccacgacg agcgagacga tattgcgctc ggccgcgggc ggcgcaaagg
     1321 ccggcaaatc cgcctgctgg agttgccggg cgctgaaacg gcgttcccat tccgcctggg
     1381 catgttgccc ttcggtgggc gagtggtagg tggcgacgat ttcgcgcgcg agaagctttt
     1441 ttgcctccat cggatgcagc tccacgggct gtgcgcgccc gaggagcagg gaatagtaac
     1501 gcgccatcaa ttcatccgag atgctcatca gtttgccaaa catgtcgtta ggggaatccg
     1561 tcactccgac atagttcctg aggctcttgc tcatcttttg caccccgtcc agcccctcga
     1621 ggatggggag gacgagaacc acctgttgcg gctggccctc ctccttttgc aggtcgcggc
     1681 cgacgagaat attgaagagc tggtcggttc ccccgagttc cacgtcggcc tgcacctgga
     1741 ccgaatccca cccctgcatg atcggatact gaatctcgtg cgctctgatc ggttgctgtt
     1801 ggtcgatccg ctgcctgaaa tcctcccgct gcaacatctg ctgcaaggtc acgcggctgt
     1861 tcagccggat cacctcggca aacgacatcg ctttgaacca gtcaccgttg aaaaccgtcc
     1921 gcgtccgctc gggatcgagc accttgaatg cctgggtgcg gtagctttcc gcgttggcca
     1981 gcacctcctc gtgggtcagt tgtggccggg tgacggagcg gccgcttgga tcaccgatca
     2041 tggccgtaaa atcgccgatg atcaggatcg cctcgtggcc gagatcctgg aattgtctca
     2101 gttttcgcag caggagggtg tgaccgagat gcaggtcggc gctggtggga tcaaccccga
     2161 gcttgacgcg gagcggccgg cctaacgaga gcttttggcg cagctcggct tcgctgatga
     2221 tctgcgccgc gccgcgtttc agaagtgcaa acgattccgc cgggtccatc acgcaagttg
     2281 tgccagcttg gggcggcttc aatcaacgcg caacgatcaa tccggcgaac cgctacctgc
     2341 tcttgttcag cagctcgcgg acttcgtcta tcgtcaggcc cttatcctcc cggctgagaa
     2401 tttcttgtcg cgtcccttcg cccaggaacg gccggcagtc cgcgtaggca cgcatcctta
     2461 cgaccttgcc ttccgcggcc gccgagcgca cgagggcgct ttccctcgtc gggaagcggg
     2521 cgttggcata cgcgagttgg gccttggtcc tggcgttcgc ggtcgcctcc gcgcggtaaa
     2581 attttccagt cgcaaaatct ttggaggaaa aagctttcga gccccagaag ctcctggtgg
     2641 ctctctcgtc gccggaataa aatgatttag tgacgaattt cttgtcggcc ggcgttccgc
     2701 cgccggcggt aaatttcctg ccctgcgccg ggttggcgag cgacatatca ggtcgtaaga
     2761 gacggtcagc aaatttgggt tcctgcttcg ggacctgcgc ggaggcgaag ccggtcgtga
     2821 gagtgaggaa caggaagaag gcgcgcacgc cgcgaccctg gggcaggcgg gcgagcgctg
     2881 taaagggaaa ttcgccgctg gtagtgggtc gatctactac ctcgccgctg gttgcgccct
     2941 aacgatcgag cagattgacc aggcgttggg ctcccggtgg tggtgccacg cttccactca
     3001 ggattgcgcg ggcgcccacc ggcctcccgt agtagcgctc attcgctgtc ttctgcgtcc
     3061 cgatgatcgc gccttcgagg gagatcccag cgaacaatcc cgcgcttcgg ctgtaggtgt
     3121 aaaccgcggc ggttggcgta acgccggcct gcaaatcgcg cccgaccgga cccgccgccg
     3181 cgctcacgtc cgcgcccagt ttcaggttcc cgtcgcgcga gaaggcgcgg accgctgcat
     3241 cattgttgag cacgaagatg aaatcagtca gttgcgcccc gatttgcaga ccccagcctg
     3301 ctccgcccag accgatgaaa gagggacccg accagccgtt ggcatcgcgc gccacgacca
     3361 cgccttcgcc tccattgccg gacacgacga agcccgcctt gagcacgcga ataatggcga
     3421 gaccgtgcgc ctcgcgcagg atgcggcgcg gaatctggtg ctccggcatc tgatggaaat
     3481 cgtggaggat attcgccgat tgattgacca cttcctgttc ggtggcggcg ctggcggcgc
     3541 tgagagctaa acaactggcg agaaggacgg atgggagttt tctgatcaca aagctatttc
     3601 gtcgccccgt ccaagggcgg acgtattgtt tgtcggccag ttttttgaaa aaggagcaat
     3661 cacgaccgga tcgaacaaaa tttttctcgc cgcgccgaat tctttagcgg cagaggcctc
     3721 attttcaagc gaataagaca ggtccatatg gaaacgatca tcgatctgca gagcgatgcc
     3781 tatttcatga gcgaggcgct ccggatggcg gcgcgagctt acgaagcgga ggaagtgccg
     3841 atcggtgcgg tggtggcgcg gcagggaaaa atcatcgcgc gcgcctgtaa ccaggtcgaa
     3901 ctgttgaaag acgcgacggc ccatgcggag atgctggcgc tgacccaggc ggaggccgcg
     3961 ctcggcgatt ggcggctaac ggattgcgat ctctttgtga ccaaggagcc gtgcccgatg
     4021 tgcgccggcg cgctcgtcca cgtgcggatg cgccgggtgg tcttcggctg cgccgacccg
     4081 cgcggcggcg cggcaggggg actattgaat ctgctccaaa acccgtcgct caatcaccaa
     4141 tgcgacgtca ctgctggcgt tctgcgcgac gaatgtgcgg aactgctgcg aagtttcttt
     4201 cgggcgaagc gatcacgcga agccgaggca cagtgagacg atcctactgc ggctccagat
     4261 cgtagacttc gacgagcgcg atgccggtct tgaaattggc gtcgcggacc accgccgtgt
     4321 aactgccggg tgaaagggtt tcgacgatgg ccgactccag gttgttcgtg ggcggaatcg
     4381 tggtggcgat gatttgttgt tcctgggtgt ctctccaatt gtcattggcg tcgaggaggg
     4441 tgccgttgct atcgtgcagc tccaaatacg gatcgggcaa gggagtcggc acgccgagcg
     4501 cgccgagtga gggaccgagc gcacgcacga tcaccgacgt cggatctgtc ccgccaacca
     4561 taaacccgcc gatgagcacg tcgttgggtg tcacgatgtc accccgggtc gagatattgg
     4621 tcacggtgga gttagccggg gtcaggtcat aggcctcaac caaagcgatg cccgtatcgt
     4681 tacccgcccc ttgaaggatg gcggtgtaac tgcccgccgg aaggatggca gtcagcagcg
     4741 attcggagga gttagcgggt tgcaaaggat ttgggatgcc agggatcacc gtccaattgt
     4801 cgttggattc caccagaact ccggcggagt cgta
//
LOCUS       DATMNS010000296         2798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4177027, whole genome shotgun sequence.
ACCESSION   DATMNS010000296 DATMNS010000000
VERSION     DATMNS010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4177027"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VNW28_10595"
     CDS             complement(<1..205)
                     /locus_tag="VNW28_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10422.1"
                     /translation="MPDVRGKKRVDGLEHISLGDWIARWVAEVSNLTIYIGFWIEKGV
                     SQIKPSVGSEAIGPGNALGQIREL"
     gene            290..2374
                     /locus_tag="VNW28_10600"
     CDS             290..2374
                     /locus_tag="VNW28_10600"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HXA10423.1"
                     /translation="MTENPLLKESSLDFHYPPFDRIKDADFAPAYEKGMAEQLKEIEP
                     IAASTEAPTFENTIVALEKTGDLLGRVDRIFSNLSAANTNPALQKTETEMAPKLSAHQ
                     DAIFLNGPLFKRIATLYNERAKLGLDEESTWLIERYYKDFVRAGAKLSDADKAKLRKM
                     NAELAALQTKFSQNVLKEKNAESIVVAKREELDGFSPTELAAASAAAREEKQVGKFVI
                     PLQNTTQQPALTDLKNRALRERIMKVSLARNSHGGQWDNRAVVLRMAKLRAERAALLG
                     YASHAAYQLEDQTAHDVGTVNKLLAQLAPPAVANARKEAADMQKLIDAEKGGFQLASW
                     DWAYYSEKVRQARYAFDEAEIKPYFELNHVLLDGVFYAATQLYGITFKERRDLPVYQP
                     DVRVFDVFDKDGKPLAIFLADYYARPSKRGGAWMNEYVNQSQLLGRQAVVANHLNIPK
                     PEAGQPTLLTYDEVRTAFHEFGHALHGMFSNVKYPRFSGTSVPRDFVEYPSQVNEMWA
                     TWPEVLQHYAKHYQTGAPMPKELLDKVLAAEKFNQGFKTTEYLAATLLDQAWHQLKPS
                     EVPNDALAFEASALKKAGVDFAPVPPRYRSTYFSHAFAGGYSAGYYSYIWAEELDADS
                     VEWIKEHGGLKRENGDHFRDTLLSRGGSKDAMKLFHDFTGRDPWIEPLLERRGLAPVP
                     QARSNEAPPPAK"
     gene            2426..>2798
                     /locus_tag="VNW28_10605"
     CDS             2426..>2798
                     /locus_tag="VNW28_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10424.1"
                     /translation="MKKDEKNRKNATDGKYASHPSHLSHPSPSSSSHKPERLLPPRGD
                     YQTLLSFQKAEIVYDFTFRFAHKFLSRGDRTIDQMVQSARSGKKNLLEGSKAALTSKE
                     TEIKLTNVARASLEELLDDYKD"
BASE COUNT          630 a          898 c          761 g          509 t
ORIGIN      
        1 cgagctcgcg aatttggcct aacgcattcc ctggaccgat tgcctccgaa cctaccgatg
       61 gcttgatctg tgaaacacct ttctcgatcc aaaacccgat gtaaatagtc aggttcgata
      121 cctcagcgac ccacctcgca atccaatcgc ctaacgaaat atgttcaaga ccatccactc
      181 tttttttccc acgcacgtcc ggcatctcgc cctcttttgc gcgccgctcc ttctcctttc
      241 cgcgtccgcc gcggatgagt cttcgttacc ttccacaagt gccacatcca tgaccgaaaa
      301 cccgttgctc aaagaaagtt cgctcgattt tcattaccca ccgttcgacc ggatcaagga
      361 tgcggatttc gcgccggcct acgaaaaagg aatggcggaa cagttgaagg agattgaacc
      421 gatcgccgcc agcaccgagg ccccgacatt tgaaaacacc atcgtcgccc tcgagaaaac
      481 cggcgacctg ctcggtcggg tcgatcggat tttttccaat ctctcggccg ccaacacgaa
      541 tcccgcgttg cagaaaaccg agaccgagat ggcgccgaag ctctccgccc accaggacgc
      601 gatcttcttg aacggcccgc tcttcaaacg catcgcgaca ctctataacg agcgcgccaa
      661 acttggtctc gacgaagaat cgacctggtt gatcgagcgt tactacaagg atttcgtgcg
      721 cgccggcgcg aaactctccg acgccgacaa ggcgaagctc aggaagatga acgccgagct
      781 tgcggcgttg cagacgaagt tttcgcaaaa cgtcctgaaa gaaaaaaacg cggagtcgat
      841 cgtcgtcgcg aagcgcgaag agctggacgg cttctcgccg acggagctgg cggccgcgag
      901 cgccgcggcc agggaagaga agcaggtggg caagtttgtc attccgctcc agaacacgac
      961 gcagcagccg gcgttgaccg atctgaaaaa tcgcgcgctg cgcgaacgca tcatgaaggt
     1021 ctcgctcgcg cgcaacagtc acggcggcca atgggataat cgcgccgtcg tcctgcggat
     1081 ggcgaaattg cgcgcggaac gcgctgcgct cctcggctac gcgagccatg ccgcctacca
     1141 actggaagat cagacggcgc acgacgtggg gacggtcaac aagttgctcg cccagctcgc
     1201 gccgcccgcg gtcgccaacg cgcgcaagga ggcggccgac atgcagaagt tgatcgacgc
     1261 ggagaagggc ggcttccaac tcgcctcctg ggattgggcc tattactccg agaaagtccg
     1321 gcaggcgcga tacgccttcg atgaagcgga aatcaaaccc tatttcgagc tcaaccacgt
     1381 cctgctcgac ggcgttttct acgccgcgac ccagctctac ggcatcacct tcaaggaacg
     1441 gcgcgatctc ccggtctatc agccagacgt tcgggtcttc gacgtcttcg acaaggacgg
     1501 caagccgctc gcaatcttcc tggccgacta ttacgcgcga ccctcgaagc gcggcggcgc
     1561 ctggatgaat gagtacgtca accagagtca attgttaggc aggcaagcgg tggtcgcgaa
     1621 ccatctcaac attcccaaac ccgaagccgg tcagccgact ctcctgactt acgacgaagt
     1681 gcggacggct ttccacgagt tcggtcacgc gctccacggc atgttttcca atgtaaaata
     1741 tccgcgcttc agcggcacga gcgtgccgcg cgatttcgtc gagtatccct cgcaggtgaa
     1801 cgaaatgtgg gcgacctggc cggaggtgct gcagcattac gcgaagcatt accagacggg
     1861 cgcgccgatg ccgaaggagc tgctcgacaa ggtgctggcg gcggagaaat tcaaccaggg
     1921 tttcaagaca accgaatacc tcgccgccac cctcctcgac caggcctggc accagctaaa
     1981 accatccgaa gtgcctaacg acgcgctcgc cttcgaagcg agcgcactga aaaaagccgg
     2041 ggtcgatttt gcaccggtcc cgccgcgcta tcgctcgacc tatttttcac acgccttcgc
     2101 cggtggttac agcgccggtt attacagtta catctgggcc gaggagctcg atgcggacag
     2161 cgtcgagtgg atcaaggaac acggcggttt gaagcgcgaa aacggcgacc acttccgcga
     2221 cacgctcctc tcgcgcggcg ggagcaagga cgcgatgaaa ctcttccacg acttcaccgg
     2281 ccgcgacccg tggatcgagc cactgctcga acgccgcggt ttggcgccgg tcccgcaagc
     2341 tcgctcgaac gaagcgccgc cgccggcaaa atagcgacga gtcagccaag tcatggagcg
     2401 ttgatcttgc gggccgtgcg ttgcgatgaa gaaggatgag aagaatagga agaatgcgac
     2461 tgatgggaaa tatgcctctc atccttccca tctttcccat ccctcccctt ccagttcttc
     2521 ccataagccc gaacgccttc tgccgccgcg cggcgattac cagaccctcc tctctttcca
     2581 aaaggccgag attgtctacg acttcacttt tcgcttcgca cacaaattcc tctctcgggg
     2641 tgaccgcacg atcgaccaga tggtccagtc cgcgcgttcg gggaagaaga acctcctcga
     2701 aggcagcaaa gcggcgctca cttccaagga aaccgagatc aagctcacaa acgtcgcccg
     2761 cgccagcctc gaggaattgc tcgacgacta caaagact
//
LOCUS       DATMNS010000297         5875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3827074, whole genome shotgun sequence.
ACCESSION   DATMNS010000297 DATMNS010000000
VERSION     DATMNS010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3827074"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2504)
                     /locus_tag="VNW28_10610"
     CDS             complement(<1..2504)
                     /locus_tag="VNW28_10610"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXA10425.1"
                     /translation="MIQLKPGLQPSTLAKHRKSLSRRRKTVSKKKRRLHFARVFSDPA
                     VSPFAQSEWERRTAEITDDSGKVIFKQENIEVPKSWSPLATKIAVAKYFYGDIANGTD
                     PHKAGRETSVRQLIHRVTRTITDCGLADGYFAGAQAAEIFYDELTWLCLNQHGAFNSP
                     VWFNVGLYHQYGVGKGAGAGNYFYNRETKQAERAATQYEYPQGSACFIQSVDDTMEDI
                     MQLAMSEAMLFKYGSGTGTDLSSLRSTREKLSGGGKPSGPLSFLKVYDQVANVVKSGG
                     KTRRAAKMNTLKDWHPDIEEFINAKQKEEKKAWALIEQGYDGSYNGDAYGSVMYQNEN
                     LSVRVSDDFMNAAVEDREWWTRRVRDGSPCERKEARGLLRKIAEGTHICGDPGMQFDT
                     TIHKWHTCKGTGRQNSTNPCSEYLFLDNTACNLASLNLMKFKDEAGAFDVERFKAAVR
                     VFITAQEIIVDNASYPIKEIAENSHIFRTLGLGYANLGSLIMSYGYGYDSVEGRALCG
                     AITAIMTGEAYAESARMAAAMGPFPGYHDARASGVPKPVAKDNVASTLEVIEQHRCAV
                     RDIPDNEEFAYLKEEAARVWDRAAELGQEFGYRNAQVTVLAPTGTISFLMDCDTTGIE
                     PDIALVKYKLLAGGGMLKIVNQTVRPALEKLGYNSEEIERIIAHVDAFDTIEDVTDSD
                     GSQITSGLKPEHLPIFDCAFKPFKGQRSLGYMAHLRMMAAAQPFLSGAISKTVNMPES
                     ATVDQIMNTYIEGWRLGLKAIAIYRDGSKRSAPLNTRKTRDMGGSADAVGLTDREQLE
                     RRVVELDEEIAMLRVQLNQPTRHRMSDTRMSLTHKF"
     gene            3079..3167
                     /locus_tag="VNW28_10615"
     tRNA            3079..3167
                     /locus_tag="VNW28_10615"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3115..3117,aa:Ser,seq:cga)
     gene            complement(3223..4053)
                     /locus_tag="VNW28_10620"
     CDS             complement(3223..4053)
                     /locus_tag="VNW28_10620"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HXA10426.1"
                     /translation="MNIDTLRSVPIFRTLDDASTFEIWKLLAFRPIEAGTALFQRGDP
                     GDSMFLIESGCVRISVKDADGHDAVLADLGPGEFFGEMAILDNQARSADATVTKPAKL
                     AVLASEDLRSLVRRKPDIALGILTAMTQRLRRTDNLLRHRVSRNANEEDAAHLTLADR
                     AADLIAEFGGSWKFIGASIAFSLVWIFFNSWFLFHPFDEYPYVLLNLVLGMVAGLQAP
                     IIMMSQNRQGQKDRLRADLDYRVNLKNELLLSEVVRRLEALETSISFRPNEERALRED
                     "
     gene            complement(4182..4604)
                     /locus_tag="VNW28_10625"
     CDS             complement(4182..4604)
                     /locus_tag="VNW28_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HXA10427.1"
                     /translation="MNPQIAIETATQLAVFLGNRPGALSRVCTELAAAEINIHALATS
                     DTIDHAVVRMVVSDPTKALMILGERGVLALENDVLMIESNNEPGTLATIADRLAKAGI
                     NIEYAYLASSPKSPHGLMILRPSNVEKAQRALHDLSAQ"
     gene            4977..>5875
                     /locus_tag="VNW28_10630"
     CDS             4977..>5875
                     /locus_tag="VNW28_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10428.1"
                     /translation="MFPLNRRKKRASLIKVSIALFAVSALCVIASRAYANVGDLYVTD
                     LASGSVIVYAPDGTSTTFTSGLVSPQGIAFDQNFNLYVVDAGDGGTGNGTIFKYNIVT
                     KAQSTLITGLSNPIGLALHGADLIVSENGMDRVTRVPTDGISPPSVFQLLPTPMGLSS
                     HFFNNNTFSYIANGPEVLRVGSDGTNMDIDPNDGSRATEVSTITIQAIPTPTPGEVVF
                     VSTDAGQITTLVNGVKQAPVATGIMDPRGMAFRPGPINTTDAGDLFVANTAGGQILDV
                     KKNATPIPFATPGAPNYMVFQTGT"
BASE COUNT         1178 a         1665 c         1759 g         1273 t
ORIGIN      
        1 tcgaatttgt gggtcaacga catgcgggtg tcggacatgc gatgacgcgt gggttgattg
       61 agctggacgc gcaacatggc gatctcttcg tccaattcga cgaccctccg ttcgagttgc
      121 tcgcgatccg ttagcccgac ggcgtcggcc gaaccgccca tatccctggt cttgcgcgtg
      181 ttcaagggcg cggagcgctt ggagccgtcg cggtaaatgg cgatcgcctt gaggccgagc
      241 cgccacccct cgatgtaggt gttcatgatc tggtcgacgg ttgccgactc gggcatgttc
      301 acagtcttgg aaatcgcgcc gctgaggaag ggttgcgccg ccgccatcat gcgcagatgc
      361 gccatgtagc ctaacgagcg ctggcctttg aacggcttga aggcgcaatc aaagatcggc
      421 aggtgctccg gtttcaaccc ggaagtgatc tgggatccgt cgctgtcggt cacatcctcg
      481 atcgtgtcga aagcatcgac atgggcgatg atgcgctcga tctcctcgga attgtagccg
      541 agcttctcga gcgccggacg aaccgtctgg ttaacgatct ttaacatgcc gccaccggcg
      601 agcagcttgt atttcaccag cgcgatatcg ggctcgatac cggtcgtgtc gcaatccatc
      661 aggaagctga tcgtgccggt cggggcgaga acggtgacct gcgcgttgcg atagccgaac
      721 tcctggccca gctccgcggc gcggtcccag acccgggccg cctcctcttt caaataagcg
      781 aactcttcat tgtccggaat gtcgcgcacc gcgcagcgat gttgttcgat cacctcgagc
      841 gtcgatgcga cgttgtcttt tgcgaccggt ttcggcacac cgctggcccg cgcgtcgtga
      901 taaccgggga acggccccat cgcggcggcc atgcgtgcgc tttcggcgta ggcttcgccg
      961 gtcatgatgg cagtgatcgc gccacagagc gcgcggcctt ccacgctgtc gtaaccgtag
     1021 ccgtagctca tgatgagcga accgaggttg gcgtagccga gaccgagggt gcggaagatg
     1081 tggctgttct ccgcgatctc cttgatcggg taactggcgt tatcgacaat aatttcctgg
     1141 gcggtgatga agacgcggac cgccgccttg aagcgctcga catcgaaggc gccggcttcg
     1201 tccttgaatt tcatcaggtt aagcgaagcg agattgcagg cggtgttgtc gaggaagaga
     1261 tattccgaac aagggttggt ggaattctgg cggccggtgc ctttgcaggt gtgccacttg
     1321 tgaatggtgg tgtcgaactg catcccggga tcgccgcaga tgtgggtgcc ctcggcgatc
     1381 ttgcgcagca gcccgcgcgc ttctttgcgc tcgcagggcg aaccgtcgcg cacccggcga
     1441 gtccaccatt cgcgatcctc gaccgcggcg ttcatgaagt cgtcgcttac ccgcacactg
     1501 aggttttcgt tctggtacat gacggagccg taggcgtcgc cgttatagct gccgtcgtag
     1561 ccctgctcga tcagcgccca cgctttcttc tcttcctttt gtttcgcgtt gatgaattcc
     1621 tcgatgtcgg gatgccaatc cttgagcgtg ttcattttcg cggcgcggcg tgttttgccg
     1681 ccgcttttca ccacgttcgc aacttgatcg tagactttga ggaacgagag cgggccgctc
     1741 ggtttgccgc cgccgcttaa cttttcgcgc gtgctgcgca ggctggaaag gtcggtccca
     1801 gtgccgctcc cgtatttgaa gagcatcgcc tcgctcattg cgagttgcat gatgtcctcc
     1861 atcgtgtcgt cgaccgactg gatgaagcag gcgctgccct gcgggtattc gtattgcgtc
     1921 gcggcgcgct cggcctgttt cgtctcgcgg ttgtagaaat aattcccggc gccggcgcct
     1981 ttcccaacgc cgtactgatg ataaagcccg acgttgaacc agaccgggct gttgaaggcg
     2041 ccgtgttggt tgaggcaaag ccacgtcagc tcgtcataaa agatttccgc cgcctgcgcg
     2101 ccggcgaaat aaccatcggc gaggccgcaa tcggtgatcg tccgggtgac gcgatgaatg
     2161 agctgcctga cggatgtctc gcgaccggcc ttgtgcgggt cggtcccgtt ggcgatgtcg
     2221 ccgtaaaaat atttcgcgac cgcaatcttg gtcgcgagcg ggctccagct ttttggcacc
     2281 tcgatgtttt cctgcttgaa aatcaccttg ccgctgtcgt cggtgatttc cgccgtccgg
     2341 cgttcccatt ccgactgcgc aaagggcgac accgccgggt cgctgaaaac ccgggcaaaa
     2401 tgcaagcggc gctttttttt actcacggtt ttgcggcgac gggaaaggga tttgcgatgc
     2461 ttggcgaggg tcgatggttg caggccgggt ttgagttgga tcatggcggg ttaaggcgat
     2521 cgggttgaga ataaaatgcg aaataccgtg aatagccccc cgaataactt gtgaattgca
     2581 gccaacaaca agataatggg gagccgttcg acgtatccac aagatgaagt gcaaatcggg
     2641 gcggaggcaa gcgtaaattt tcactttttt ttacccctca ttttgaacga cttgcaaacc
     2701 aaaatcgcct ccatccggct ctccaaacgg aacagaattc ggacgaaaat tcatctgttt
     2761 tcatgcgcgc ccgggtgagc cacaaaaact gaaccatgag accgaaaagg aagcgccgga
     2821 acgctggctc actcgctgcg ggggaggaga aatcgccgcg cgattcccgc caacgttagg
     2881 caaactcttt tccgcgcgcg aaaaacttga tcggtttgcg ggcttgcctc ccgtttcgtg
     2941 attccgatga ttcaatgaca accgcgcaag tccgttttcg ttaggcaatt tggcccccgc
     3001 ggttcttccg gtttgcaaat cgcgggttga atttcatagt agcgggcgac cgcaggcggc
     3061 gagttggccg caggaagagg aagggtggct gagtccggtt taaggcactc gactcgaaat
     3121 cgagcgtggg gttaaactca ccgtgggttc gaatcccacc ccttccgccg ctttttgcgc
     3181 gcggatgatg agtttcctcg gaggatccgt cgcttgatgg aatcaatctt cgcgcaacgc
     3241 ccgctcctcg ttaggccgaa aggaaatgct ggtctcgagg gcctcgaggc gcctcacgac
     3301 ctcggagagc aggagttcgt tcttgagatt tacccggtaa tcgagatcgg cgcggagccg
     3361 gtctttctgt ccctggcgat tttgcgacat catgatgatc ggcgcctgca gtcccgcgac
     3421 catcccgagc acgagattga gcaggacgta ggggtattca tcgaagggat gaaagaggaa
     3481 ccagctgtta aaaaagatcc agacgagaga gaaggcaatc gaggccccga tgaatttcca
     3541 gctcccgccg aactccgcga ttaaatccgc ggcccggtcg gccaacgtga gatgggccgc
     3601 atcttcctcg tttgcatttc gcgaaacgcg atgacgcagc aggttgtcgg tgcgccggag
     3661 gcgctgggtc atggccgtca ggatgccgag cgcgatgtcg ggtttccttc ggactaacga
     3721 acgcaaatct tcactcgcga ggacggcgag tttggctggc ttggtcaccg tggcgtcggc
     3781 cgagcgcgcc tgattatcca ggatcgccat ctcgccaaag aactcgcccg gaccgaggtc
     3841 ggccagcacg gcgtcgtgac cgtcggcatc cttgacgcta atccggacgc atccactctc
     3901 gatcagaaac atcgagtctc ccgggtcgcc gcgctggaaa agcgcggtcc cggcctcgat
     3961 cgggcgaaag gcgaggagtt tccagatctc gaaggtcgag gcgtcgtcga gcgtgcgaaa
     4021 aatcggcacc gagcgaaggg tatcgatatt catggcagtg gagtccgggc gctcagaagt
     4081 tgcaccacct gcccgggcgc gtcactgcca aaattgattg tcatcgcggc gagggtcttc
     4141 cgatcgaggc cggttcgtcc ggcaattcgc agcggcgcgg atcactgcgc cgagagatcg
     4201 tgcagtgcgc gctgcgcctt ttcgacgttc gatggtcgca ggatcatgag gccgtgcggc
     4261 gatttcgggc tcgacgccag gtaggcgtat tcgatgttga tccccgcctt ggccagtcgg
     4321 tcggcgatcg tggccagcgt gcctggctca ttgttgctct cgatcatcag gacgtcgttc
     4381 tccagtgcga gcacgccgcg ttcgccgaga atcatgagcg ccttggtcgg gtcgctcacc
     4441 accatgcgaa cgacggcgtg gtcgatcgtg tcggaagtgg cgagggcgtg aatgttaatc
     4501 tccgcggcgg cgagctcggt gcagacacgg gagagcgcgc ccgggcgatt cccgaggaaa
     4561 acagctagtt gggtggcggt ctcgatcgcg atttgcggat tcatgatgga tagaatgcgc
     4621 tcgtgctcgt cggctgacaa ccaaacaaat gcggcacctt agatcgtgag cgacgcaccc
     4681 cgatcccagc cggctgatca gggccgaatt ttcagcggtg catcgatcga tgcgagaggc
     4741 tgcaaaaaag cccgacatta aaatcaaaga cttcacaatc ggataacgca aataatgaat
     4801 cgatgtccgc ttcgttactg gaacccaaca agcctgatca aaagtggccg caaaataatt
     4861 atggacaacc gctcttaaaa taggtcatta tcgcgattgc gaagaaaata gaggcttcac
     4921 gtaattcaat tcataccatc aaacccagcg tccaatattc agaaaagaga gatcctatgt
     4981 ttccactaaa tcgacggaag aaacgcgcat cgttaatcaa ggtgagcatc gcattgtttg
     5041 cggtttcggc attgtgcgtg attgcctcca gggcatacgc caacgtaggc gacctctacg
     5101 tcaccgactt ggcctccggc tcggtcattg tttacgctcc cgatggaacg tcgacgactt
     5161 tcacgagcgg cttggtaagt ccgcagggaa tcgcctttga ccagaatttc aacctttacg
     5221 ttgtcgacgc cggtgatggc ggcacgggga acgggacgat tttcaagtac aatattgtca
     5281 ccaaagctca gagcacgttg atcaccggat tatctaatcc aatcggcctg gctcttcatg
     5341 gagcggatct tatcgtttcc gagaacggta tggaccgggt tactcgcgta ccgacggacg
     5401 gcatcagccc cccgagcgtg tttcaactcc ttccgacgcc gatggggctt tcttcgcatt
     5461 tcttcaacaa caataccttc agctatatcg ccaacggtcc cgaggtcctc agagtggggt
     5521 cggacgggac gaacatggat attgacccga atgacgggag ccgggcgacc gaggtatcga
     5581 ccattacgat ccaggctatc cctaccccta ccccgggcga agttgtcttt gtcagcacgg
     5641 atgctggcca gatcacgacg ctcgtcaatg gtgtaaaaca ggcgcccgtc gcgacaggta
     5701 ttatggatcc aaggggcatg gcattccgac caggaccgat taatacgaca gacgctggtg
     5761 accttttcgt tgccaacact gcgggcggcc agatcctcga cgtgaagaaa aacgcgacgc
     5821 cgatcccctt cgcaacgccc ggtgcgccta actacatggt ctttcaaacg ggcac
//
LOCUS       DATMNS010000298         3004 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4178943, whole genome shotgun sequence.
ACCESSION   DATMNS010000298 DATMNS010000000
VERSION     DATMNS010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3004)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3004)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3004
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4178943"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VNW28_10635"
     CDS             <1..220
                     /locus_tag="VNW28_10635"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXA10429.1"
                     /translation="DPITGTFSSITGLPAGYSATVNYAFSGTDSLGRTGDGNDLALQL
                     VPEPQSFALLAVGAALSLCVFRLRRRSA"
     gene            360..1055
                     /locus_tag="VNW28_10640"
     CDS             360..1055
                     /locus_tag="VNW28_10640"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HXA10430.1"
                     /translation="MKTLGIVGGLGPESTIEYYRFILEGYRARVPDGSTPHLLIDSLD
                     VNRAIALLDANDLAGLTNYLSAAIDRLVRAGAEVALIAANTPHLVFDEVQQRAPIPLL
                     SIVQAACDHARGLGLRRLGLLGTGFTMRARFYPDVFARAGLELVTPRADEQAFIHEKY
                     IHELLKNQFLPETRTALLAIIERMRNDQGIKALVLAGTELPLLLRGAEPEGVTFLDTT
                     TIHVNAAVDAIAN"
     gene            1611..1994
                     /locus_tag="VNW28_10645"
     CDS             1611..1994
                     /locus_tag="VNW28_10645"
                     /inference="COORDINATES: protein motif:HMM:NF021221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HXA10431.1"
                     /translation="METIFPTPPPLPTTALTNVRTWNALCHASALFGVFLYFPGHLLG
                     PLIVWLIKRGDSPEIDAHGKESLNFQISMLLYTLISGVFCLILIGFVFIAVLWVLNAV
                     FVIIASIQASDGKFYRYPMTIRFIQ"
BASE COUNT          539 a         1025 c          819 g          621 t
ORIGIN      
        1 ggatcccatc accgggacct ttagtagcat caccggcttg cccgccggct actctgcgac
       61 ggtcaattac gccttcagcg gcaccgattc gttaggccgg accggtgatg gcaacgacct
      121 cgcgctccag ctcgttcccg agccgcagtc gttcgctctg ctggcggtgg gcgcggcgct
      181 ctcgctctgc gttttccgac tgcgccggcg cagcgcctga gcatcatcgg caaccataac
      241 aattggaccg cgcctgccgg tgcgaatccg ttcggcccgt cggaaaggtg taacggcgta
      301 gcctggtcgc acgcggggcc tggccgccgg cgaatttttg aggcaatgtc tgccggcaaa
      361 tgaagactct cggcatcgtt ggcgggctcg ggccggaatc gaccatcgaa tactaccgct
      421 tcatcctcga gggttaccgc gcgcgcgtgc ccgacgggag cacgccccac ctcctcatcg
      481 acagcctcga cgtgaatcgc gcgatcgcgc tgctcgacgc caacgatctc gccggcctaa
      541 cgaactacct ttccgccgcg attgatcgtc tggtgcgcgc gggcgccgag gtcgcgctca
      601 tcgcggccaa cacgccccac ctcgtctttg acgaggttca gcagcgagcg ccgatcccgc
      661 tgctcagcat tgtgcaggcg gcctgcgacc acgcgcgcgg actcgggctc cggcggctcg
      721 gtttgctcgg caccggcttc accatgcgag cgcggtttta tcccgacgtt ttcgcgcgcg
      781 ccgggctcga gctggtcacg ccacgcgcag acgagcaggc gttcatccac gaaaaataca
      841 tccacgaact gctcaagaac cagttcctgc ccgagacccg caccgccctc ctcgcaatca
      901 tcgagcggat gcgaaacgac cagggaatca aagcgctagt cctcgccgga accgagctcc
      961 cactcctcct ccgaggcgcc gaaccggagg gtgtcacgtt tcttgatacg acgacgattc
     1021 acgtaaacgc cgccgttgac gcgatcgcga actaagccac ggattgaccc cgattaagca
     1081 ccgacgctcc gattaccgct gtctcaaatc cggatccacc tgaaagcctg accgccccgt
     1141 gcttgcgggg cttcgttacg ggtggctaaa ctcccagccc gcgcgaaatt gaaagcatga
     1201 atactcaatc gcgaaccctg cgcgcgttgg ccatgggcac agcttctctc ggcgcattgg
     1261 cggtgggcgc cgttgcgctt ggcgcgctgg cgattggcgc gttagccatc ggcaaattgg
     1321 ccatcggccg agcgcgaatc cggcgacttg aaatcgatga actcgtcgtt cgagaccttc
     1381 gcgtgaccga aaaaatcgac gccccgacag agccggctcc gagccgctaa taattcgccg
     1441 acggcaatcg gctaaaaacc ccatgaccgc cgcgtgcccc agtcctgccc gtttcctgcg
     1501 tttctgattc aagtttcgag ctctcggttt cctggcttcc aaattcaaat catctgcggc
     1561 aaattcttga atccaaaccg gcgttcggcc gcatccatcc agtagacacc atggaaacga
     1621 tcttcccaac cccaccgcca ctcccgacaa ccgccctcac caacgtgcgc acctggaatg
     1681 cgctttgtca cgccagcgcc ctgttcggcg ttttccttta tttccccggc cacctgctcg
     1741 ggccgctcat cgtctggctg atcaagcgcg gcgactcgcc cgagatcgat gcgcacggca
     1801 aggaatcgct caactttcag atctcgatgc tcctttacac cctcatctcc ggggtctttt
     1861 gcctcatcct gatcggcttc gtcttcatcg cggttctttg ggtgctgaac gccgtcttcg
     1921 tcatcatcgc ctcgatccag gcgagcgacg ggaagttcta tcgctacccg atgacgattc
     1981 ggtttatcca gtaatcccga gcaacgaacg ttcgagccta accaaattgt agctcaacat
     2041 agctaccggc cttcatggcg acgaccgcgg atccgatttt ctcacttccg ctaccctcgc
     2101 ggcaacccat ctgcgtcgcg cgccgcgctc gctccttcca gctttcagtt ttcagcgttt
     2161 gattcgctcg cgcccgctcg ctcacccttc gggcgtcgtc tatgtcgttc gcccccctcg
     2221 ctccattcag cttttctcac ggcctagaat ccgagggaga actgcgcacc gtttacctgt
     2281 cccataacgg gttcaaccgc gatatcgccg agtttcgaat tgatgtggaa gagcgtgagc
     2341 ggattatggt cggtcgcgtt ttccctctcg ctgttccggt tcgatccgtg ctccgcggag
     2401 gccgaagatc tggctgcgaa aggccttacc tccgaagcgc caaacatctc ggatcggagc
     2461 gatacctcgt gagccttcgg agcaacgagc atctccttcg cctcgtggac ccgcgtcgcg
     2521 gacttccgat cgcccgcgtg cgccggttgg caggcgagtc cgagcgcgac caacaaccca
     2581 caggctccaa cgagatttct ccccatcgct cctcccattt ttgattcgga ttttccgcgc
     2641 gaactgcgcg cgcggaagtg accaaccgtg gattggcgtc cgccgcacat ccgagaatcg
     2701 gatgcgatgc aacccggaat cggccagcgc ccgggctgcc caaccgaatt cgtccaaaaa
     2761 ctcctcgccc agaccagcct gctgttcgtt ggaccggggg acttcgagcg cgtcccgatc
     2821 agctgagagg aagttttctg acgacgaaac tcctgcaatc gctcgcgtgg gcttgcctga
     2881 tttgacgggc agtgggctta gagggatatc acttcgctgc tgcttccttg ctcccgggtt
     2941 cggcgttcaa cagtttctgg aagcgtggat ccttgcgcag tgggtcccag aaaggatcaa
     3001 gtct
//
LOCUS       DATMNS010000299         5771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4179642, whole genome shotgun sequence.
ACCESSION   DATMNS010000299 DATMNS010000000
VERSION     DATMNS010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5771
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4179642"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(466..954)
                     /locus_tag="VNW28_10650"
     CDS             complement(466..954)
                     /locus_tag="VNW28_10650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10432.1"
                     /translation="MFRNFSPFSGWFGLCALGFSVFAQSADNAVRVTVAINPDGSKTV
                     YQTDGVNRQAVATTTDANGKARGKIIYQLDAQGRYESGQVFGANSALRFKTLYQYDPS
                     GRLAQEMQLAKDDSVRHKIIYSYDGAGHQAGYAVYNGDGKLLGRTTAKIEDARSTPHR
                     KP"
     gene            1081..1539
                     /locus_tag="VNW28_10655"
     CDS             1081..1539
                     /locus_tag="VNW28_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10433.1"
                     /translation="MEPASSSLIFYAGEFRHQLDEKHRLTIPARWRRNGESEEFLIVP
                     EPSGQFLLVMPPNEFARISSAAETVPGVSPRDLRIYLRHLHSRAQHGSSDKQGRLLLP
                     DDLCKQLDLKGEVALVGGRGRFEIWNLPRWKRAHAEEHATYQHVANLVGL"
     gene            1545..2546
                     /gene="rsmH"
                     /locus_tag="VNW28_10660"
     CDS             1545..2546
                     /gene="rsmH"
                     /locus_tag="VNW28_10660"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HXA10434.1"
                     /translation="MNALLFERPIRFATGPLLINEEAQDMEDFTYHRPVLASEAIELL
                     APRPGSLILDGTCGGGGHTEAILRTGADVVALDQDPDALEFAGARLTNFGRRVTLRRA
                     NFREAGRVLDELGIAQIGGAILDLGVSSRQLENADRGFSMMRNGPLDMRMDPRRDLTA
                     AEVVNSYTEEELTRIFREFGEEPAARRIASQIVKLRKEAPFRETLALAKAVEKIVGRH
                     GRRHPATQVFQALRMEVNDELGALAEGLAGLTNRLESGGRLAVITFHSLEDRIVKNFF
                     RDRSREFLDRPEWPAPRPNPEFVLQVITPKPVEPDAVEQRANPRSRSAKLRVAERIR"
     gene            2543..2911
                     /locus_tag="VNW28_10665"
     CDS             2543..2911
                     /locus_tag="VNW28_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10435.1"
                     /translation="MNRRRRKNFNPIDAPSLARWLVIATFMAATGLSYVYLSVQLHHQ
                     GVQRRQLEQDLATVRMQNEDARVQIAALTSRTALQRRLKEGYLKMIPITEQSIVRLAS
                     PVRLPGEDDVQPVVNQRGGQ"
     gene            2908..4674
                     /locus_tag="VNW28_10670"
     CDS             2908..4674
                     /locus_tag="VNW28_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXA10436.1"
                     /translation="MKWNGRARCALVCVFFALLFSVFSWRLIYLQMVMHGYYSELAAE
                     KNVGRNIIPAERGAIYDTHSEVLAQNVPVATVVADGTLITRADALVPLLAAKLKLPPN
                     EIAEKIRSRRPYIVLKRELPEAQARALEQSLKAEKLCGVRCERSTVRVYPNGPMLCHV
                     IGFSDFQQHGIQGVEASMDQYLRGEDGYRYIEHDAQGREIVLYRGLEHEPRNGYQVHL
                     TIDLNLQTIVENELDAAMREYTPEKATIILMRPETGEILALANRPNFDLNLRATARPE
                     QMKNRAIIDMMEPGSTFKIVTAAAALNERKVRLDSMIFCENGVWNFGGRPLHDHGHKG
                     YGDLSVQDILVHSSNIGAAKMAVMLGEQKFYEYIRRFGFGDRTGIELPGEIGGMVHSP
                     KSWSKISITRIPMGQEVGVTPLQMLMAMATVANGGKLLQPRIVKSITDENGKAISVLQ
                     PTVVRQVVSPETAAQIALALRGVVSQRGTAAEAAVPGFTISGKTGTAQKAAPGGGYEK
                     DKYVVSFSGFLPSEHPAFVGLVVLDDAHTKDPALNYGGTIAGPIFSRVAEKAARYLDL
                     EPHEEVGTQVAAGRVALTNADR"
     gene            4703..>5771
                     /gene="murE"
                     /locus_tag="VNW28_10675"
     CDS             4703..>5771
                     /gene="murE"
                     /locus_tag="VNW28_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HXA10437.1"
                     /translation="MQLKILAESIPVQEVVGPLDREVDGIFYDSRRVQKNGLYVAVRG
                     AHFDGHQFTEQAIERGASAIVVERPESHSRATSLVVADSRVALADLAFTFFKKPALRL
                     KMAGVTGTNGKTTTTFLLKHICETAGLRSGLIGTVRYEIGERVLPATRTTPESLDLQE
                     LLAQIAGAGCQAAILEVSSHALAQERTRGLEWDVAIFTNLTQDHLDFHQTMENYFEAK
                     ARLFTGLQSQPNKKQATAVINLDDRYGEQLVSRVRDETPVVTYGMSARADFRASNYRA
                     EFGGTSFQLDARGKIYLVRVPLIGRFNVSNTTAALAAASSLGINLRAAVLSLAKAPQV
                     PGRLEAVLAKRQFQVFVDYAHT"
BASE COUNT         1146 a         1774 c         1779 g         1072 t
ORIGIN      
        1 gaattcgccg acgtgaatcg tggcgcgatg gaagtaaaaa aggcgctccg cgaagacagc
       61 gatcgaaatg atgctgcaga cgagaatagg ccacatgagc agaccaccct tctggatgta
      121 atcgatcatg cgttgacctg ataactccct ttgccactcc tggcaagtgg agaggtcggc
      181 gacctaacga gtaaactaaa ggaaggctgc gcgccgcgaa ggagcgatga acctcgcggc
      241 gcctggtcga tacttcgcgc tgcgaagcgt gacaggcgcg ttgtagccga cggcggggac
      301 accggttggt cgagcgcagg gcaacgttta atcgcggccc cgaagtcaac gccccggcta
      361 caacctgtat cccaacgaac gcttttcgcg acgccaaaca gaaatctttg cggagcgaag
      421 cgtcgaccag ggcggcgcgg cgtttctttc ggtttcattc gcttcctatg gtttgcgatg
      481 tggagtgctg cgcgcgtctt ctatcttcgc agtcgtccgc ccgagcaact tgccgtcgcc
      541 attatagacg gcgtaacccg cttgatgacc cgcgccatcg tagctgtaga tgatcttgtg
      601 gcggacggaa tcatccttcg cgagctgcat ctcctgcgcc agccggccag aagggtcgta
      661 ctgatagagc gtcttgaaac gcagcgcgct attcgccccg aacacctggc cgctttcgta
      721 gcgaccctgg gcgtccagct gataaataat cttaccgcgc gctttcccgt tggcgtcggt
      781 cgtggttgcg accgcctgcc ggttgacgcc atcggtttgg taaacggttt tcgagccatc
      841 aggatttatc gccacggtta cgcgaaccgc gttgtcggcc gattgggcga agaccgagaa
      901 accaagcgcg cagaggccaa accagccgga gaaaggagaa aagttccgga acatgcgtcg
      961 agcgcgaaat ttacggggtc aaagaaaagc gtgcaaacgg gcatttattt attgaccgga
     1021 gaaaatcgga gtggtttaaa gtggggggaa gtggattaat ttggaacaat cctcctgcgc
     1081 atggaaccgg cctcttcatc tttaattttc tacgcgggag aatttcgcca tcaactcgac
     1141 gagaaacatc gcctcaccat cccggcgcgc tggcggcgca acggcgagag cgaagagttc
     1201 ctcatcgtgc cggaaccgag cgggcaattt ctgctcgtca tgccgcctaa cgaatttgcg
     1261 cgcatcagct cggcggccga aaccgtcccc ggagtctcgc cccgcgatct gcgcatttat
     1321 ctgcgccacc tgcattcccg cgcccaacac ggctcgtccg ataaacaggg ccgtctgctt
     1381 ttgcccgacg acctgtgcaa acaacttgat ctcaagggcg aggtcgcgct cgtcggcggc
     1441 cgcggtcgtt ttgaaatctg gaacctgccc cgctggaagc gggcccatgc cgaggaacat
     1501 gccacctacc agcacgtggc caatctggtc ggactctaga ggaaatgaac gcgttgttat
     1561 tcgaacggcc catccgcttc gcgactggtc cgctgctgat caacgaggag gcgcaggata
     1621 tggaggactt cacctatcat cgaccggttc tcgcgagcga ggcgattgag ttgctcgcgc
     1681 cgcgtcccgg ctccctcatt ctcgacggca cctgcggcgg gggcggccat accgaagcga
     1741 tcctgcgcac cggcgccgac gtcgtcgctc tcgaccagga cccggatgcg cttgaatttg
     1801 ccggcgctcg cctgacgaac ttcggccggc gggtgacgct gcgacgggcc aatttccgcg
     1861 aggcgggccg cgtcctcgac gaacttggga ttgcccaaat cggcggcgcg attctcgacc
     1921 tcggcgtttc ctcgcggcag ctggaaaacg ccgatcgcgg tttcagcatg atgcggaacg
     1981 ggccgctcga catgcggatg gatccacggc gggatttgac ggcggccgaa gtcgtgaaca
     2041 gctacacgga agaagagctg acccgcatct tccgcgaatt cggcgaagag ccggcggcgc
     2101 gccggatcgc gagccagatc gtgaagctgc gtaaggaagc gcctttccgg gaaacgctcg
     2161 ccctcgccaa ggcggtggag aaaatcgttg gccgccacgg gcggcgccat ccggcgacgc
     2221 aggttttcca ggccttgcgg atggaagtga acgacgagct tggcgcgctc gccgaggggc
     2281 tggctggcct aacgaatcgc ctcgagtcgg gcggccgcct cgccgttatt acctttcatt
     2341 cactcgagga tcgaatcgta aaaaacttct ttcgcgatcg cagccgcgag tttctcgatc
     2401 gtccggagtg gcccgcgccg cggcccaatc cggagttcgt gttgcaggtc atcacgccga
     2461 agccggtcga gccggacgct gtcgaacaac gggccaaccc gcgttcccgc agcgccaagt
     2521 tgcgcgtcgc cgagaggatt cgatgaaccg tcgccggcgc aaaaatttca acccgatcga
     2581 cgccccctcg ctcgcgcgct ggctcgtgat cgccaccttc atggccgcga ccggcctgag
     2641 ttacgtctac ctttcggtcc agctccatca tcagggtgtg cagcggcggc agctcgagca
     2701 ggacctggct acggtccgga tgcaaaacga ggacgcccgg gtgcagatcg ccgcgctcac
     2761 ctcgcgcacc gcgctccagc gccgcctgaa ggaggggtat ctgaaaatga tcccgatcac
     2821 cgagcaaagc atcgtcaggc tggcttctcc cgtacggctg ccgggcgagg atgatgtgca
     2881 gccggtggtc aaccagcgag gcggccaatg aagtggaatg gccgagctcg atgtgcgctc
     2941 gtctgcgttt tctttgcgct cctcttcagc gtcttttcct ggcggctgat ctatctccag
     3001 atggtgatgc acggctacta cagcgagctg gcggcggaga agaacgtcgg tcgcaatatc
     3061 atcccagcag agcgcggcgc gatctacgac acccacagcg aagtgctcgc gcagaacgtc
     3121 ccggtcgcca cggtggtggc cgatggcacg ctcattacca gggccgacgc gctcgtccca
     3181 ctgctcgccg cgaaactgaa gttgccgccc aatgaaattg cggaaaagat caggagtcga
     3241 agaccctaca tcgttctgaa gcgggaactc ccggaagcgc aagccagagc gctggagcaa
     3301 tccttaaaag ccgaaaaact ttgcggcgtc cgctgcgagc gcagcacggt gcgggtttat
     3361 cccaatggac cgatgctctg ccacgtcatc gggttttccg attttcagca gcacggcatc
     3421 cagggcgtcg aggcgtcgat ggatcaatat ctgcgggggg aggacggtta tcgctacatc
     3481 gagcacgacg cgcagggacg cgaaatcgtc ctctaccgcg ggttggaaca tgagccgcgc
     3541 aacggttacc aggtccacct cacgatcgat ctgaatttgc agacgatcgt cgagaacgaa
     3601 ctcgacgccg cgatgcgcga gtacaccccg gagaaggcga ccatcatcct gatgcggccg
     3661 gaaacgggtg agattctcgc gctggcgaat cggcctaact ttgatctcaa tcttcgcgcg
     3721 accgccaggc cggagcagat gaagaaccgc gccatcatcg acatgatgga acccggctcg
     3781 actttcaaga ttgtgacggc ggccgccgcg ctcaacgaga ggaaagtccg gcttgattcg
     3841 atgatcttct gtgaaaacgg cgtctggaat tttggcggcc gtccgctgca cgatcacggg
     3901 cacaagggtt acggcgacct gagcgtgcag gatatcctgg tgcattcgag caacatcggg
     3961 gccgccaaaa tggcggtgat gctgggcgaa cagaagttct acgaatacat tcgccgcttc
     4021 ggttttggcg atcgcaccgg gattgagttg ccgggcgaaa tcggcgggat ggtgcattcg
     4081 ccgaagagct ggagcaagat ttcgatcacg cgcattccga tgggacagga ggtcggagtg
     4141 actccgctcc agatgttgat ggcgatggcg acggtggcga acggcggcaa gctcctccag
     4201 ccgcggatcg tgaaatcgat caccgacgag aacggaaaag cgatcagcgt tttgcaaccg
     4261 accgtcgtga ggcaggtcgt ttcgcccgaa accgccgccc agatcgcgct cgccttgcgc
     4321 ggcgtcgtca gccaacgcgg aacagcggcg gaagcggctg tgccgggttt cactatttcc
     4381 ggcaagaccg gcaccgcgca gaaagccgcg cccgggggcg gttacgaaaa ggacaagtat
     4441 gtcgtctcct tcagtggctt cctgccctcg gagcatccgg cctttgttgg cctcgttgtg
     4501 ctcgacgacg cccacaccaa ggacccggcc ttgaactacg gcgggaccat tgccggaccg
     4561 attttttccc gcgtcgcgga gaaggcggcg cggtacctcg accttgagcc gcatgaggaa
     4621 gttggaacgc aggtcgcggc tggacgagtc gccttaacta atgccgatcg ctaagtcccc
     4681 gcgttgcgcg tgtccacacc tcatgcagct caaaattctc gccgagtcca ttcccgtgca
     4741 ggaggtcgtc gggccgctcg atcgcgaagt agacggcatt ttctacgatt cgcgccgggt
     4801 gcagaagaac ggtctctacg tcgcggtgcg cggcgctcat tttgatggcc atcaattcac
     4861 cgaacaggcg atcgagagag gcgcgtcggc gatcgtggtg gaacgcccgg agtcgcattc
     4921 ccgcgccacc tcccttgtcg tcgcggatag tcgcgtggcc ctggcggatc tcgccttcac
     4981 ttttttcaag aaaccggcgc tccggttaaa gatggccggc gtcaccggga cgaatggcaa
     5041 aacgacgacc accttcctcc tcaagcacat ctgcgagacg gccgggttgc gcagcggctt
     5101 gatcggcacg gtgcgctacg aaatcggcga gcgcgtcctg cccgccacgc gaaccacgcc
     5161 cgagtcgctc gatcttcagg aattgctcgc gcaaatagcg ggcgcgggct gtcaggccgc
     5221 gatcctggaa gtctcctccc acgcgctggc gcaggaacgg acccgcggcc tcgagtggga
     5281 cgtcgcgatc ttcaccaacc tgacgcagga ccatctcgat tttcatcaga cgatggaaaa
     5341 ttatttcgag gcgaaggcgc gccttttcac cggcctgcag agtcagccta acaagaagca
     5401 ggcgaccgcg gtgatcaacc tcgacgatcg ctacggcgag caactcgtca gccgggtgcg
     5461 ggatgagacc ccggtcgtca cctacgggat gagcgcgcgg gccgattttc gggcttcgaa
     5521 ttaccgggcg gagtttggcg gcacttcctt tcagctcgac gcgcgcggca aaatttatct
     5581 cgtccgcgtg cccctgatcg gccgcttcaa tgtttcgaac acgacggccg cgctcgccgc
     5641 cgccagctcg ttaggcatca atctgcgcgc tgccgtcctc agcctcgcca aagcgccgca
     5701 ggtgcccggc cgcctcgagg ccgttctcgc aaaacgccag tttcaggtct tcgtcgatta
     5761 cgcgcacacc g
//
LOCUS       DATMNS010000300         2943 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3548071, whole genome shotgun sequence.
ACCESSION   DATMNS010000300 DATMNS010000000
VERSION     DATMNS010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548071"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VNW28_10680"
     CDS             complement(<1..710)
                     /locus_tag="VNW28_10680"
                     /inference="COORDINATES: protein motif:HMM:NF013703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HXA10438.1"
                     /translation="MRSFIPSLDDLRRTVRLAAPVAFVQVGMMAMGMVDIIMVGHVSG
                     VALAAVALGNLYFFGTVIFGMGVLMALDPVIAQSVGAGDNLGVSRGLQRGLILAVALT
                     IPATPIMLAAGPVLHWLAQPPDVVPLAARYDWISIPGILPFFAFLVLRQTLQAQHRMR
                     SIVLTIIISNLLNAALDWVLIFGHLGFPASGVVGSAWATMLSRWFMAFAMLGAGWSGL
                     GGYLFPIQPRALELAPFRR"
     gene            complement(852..1580)
                     /locus_tag="VNW28_10685"
     CDS             complement(852..1580)
                     /locus_tag="VNW28_10685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10439.1"
                     /translation="MLLLFVAIVRRCSAQGGPPMITDDPGTPGNRKWEINLAVTLEHR
                     PGETSLDLPEVDLNYGIGENIQLTLQTAPVLLDREGHGPIAGLGGTEAAVKWRFLDEA
                     TSGIDASMFPRIIFNVVQSSVRRGLAEDGTRFQIPIELAKKFGVFHADVEGGPLVSTV
                     GRGEWLYGVVGGIDISKTTTLMAELNGTSRTNFTRDSLVVNFGLRHEFSETRIFITSL
                     GHEVRSSGDQPLAWIGYFGMQLIY"
     gene            complement(1941..2312)
                     /locus_tag="VNW28_10690"
     CDS             complement(1941..2312)
                     /locus_tag="VNW28_10690"
                     /inference="COORDINATES: protein motif:HMM:NF042735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXA10440.1"
                     /translation="MNTEEVAKKVVELCRKQAWNEAIELLYADDVVSVEARTMDGSSP
                     ETRGIQGVREKVEWWKTNMEVHGVKVGEPFVSHDRFVVPFDLDVTDKNSKNRMQMSEV
                     GVYTVKDGKITHEQFFPAVGP"
     gene            complement(2587..>2943)
                     /locus_tag="VNW28_10695"
     CDS             complement(2587..>2943)
                     /locus_tag="VNW28_10695"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXA10441.1"
                     /translation="DRKGADVVYDSVGATLDASLTVTHKGGQVVFYGMTGGDPAPVDP
                     RRLMDESKTLTGGDLWNVLTSRGERIARAAELFGWVRAGKLKVRIAEEFPLRDGAKAH
                     AYLESRQSIGKVLLIP"
BASE COUNT          611 a          923 c          849 g          560 t
ORIGIN      
        1 cggcggaagg gtgcgagttc aagcgctcgc ggctggatgg gaaaaagata gccaccgaga
       61 ccggaccaac ctgcgcccaa catggcgaaa gccatgaacc agcggctcag catcgtagcc
      121 caggccgagc ccacgacccc gctggccgga aatccgaggt gaccgaagat caacacccaa
      181 tcgagggcgg cattgagcag attcgagatg atgatcgtga gtacaatcga cctcatccga
      241 tgctgcgcct ggagcgtctg gcgaagaacg aggaaggcga agaacggaag gatcccggga
      301 atacttatcc agtcgtagcg cgccgcgagc gggaccacat cgggaggctg cgccagccag
      361 tgcaaaacgg gacccgccgc cagcatgatc ggagtcgccg ggatggtgag cgcgaccgcc
      421 aggatcaagc cgcgctgcag accgcggctc accccgaggt tgtctcctgc accgaccgac
      481 tgcgcgatca cgggatcgag cgccatcaac acgcccatcc cgaagatcac ggtgccaaag
      541 aaatagagat tacccaacgc gaccgcggcc agcgccacgc cggaaacgtg tcccaccatg
      601 atgatgtcga ccatgcccat cgccatcatc ccgacctgca cgaaagcgac cggtgcggcg
      661 agccggacgg tgcgacgcag atcatcaaga ctgggaatga aggagcgcat gtcggtcgac
      721 ggtggggcaa gcccgtttcc agaaaggcgg actatcggcg gagaaggttg gggaaacaac
      781 cggccggcgc ttacagatcg atctgcggcg actggtcaac acgttcggca cactgcaccg
      841 gggcgtctcc ttcagtagat caactgcatt ccaaaatagc cgatccatgc gagcggttgg
      901 tcgccgctcg agcgaacttc atggccgagc gaggtaatga agatgcgcgt ctcgctgaat
      961 tcgtgacgca gcccgaaatt caccaccagc gaatcgcgcg tgaaattcgt gcgcgacgtg
     1021 ccgttcagct ccgccatgag cgtcgtagtc ttcgaaatgt cgatgccgcc gacgacgccg
     1081 tagagccatt cgccgcgacc gaccgtgctg accaggggac cgccttccac gtcggcatga
     1141 aaaaccccaa attttttcgc gagctcgatt ggaatctgaa aacgtgtgcc gtcttccgcg
     1201 agcccgcgtc gcaccgacga ttgcacgacg ttgaaaataa tgcgcggaaa catcgaggcg
     1261 tcgatcccag acgttgcctc atcgagaaag cgccatttca ccgcggcctc tgtgccgccg
     1321 agcccggcga ttgggccgtg accctcacga tcgagcagca cgggcgcggt ttgcagcgtg
     1381 agctggatat tctcgccgat gccgtagttc aaatcgactt cgggcagatc gagcgaggtc
     1441 tcgcccggcc ggtgctccag ggtgacggcg agattgattt cccacttccg gttccccggc
     1501 gtgccgggat cgtcggtaat catcggcggc ccgccctgcg cggagcagcg ccgaacgatc
     1561 gccacgaaga gcagcagcac tatcgttaga ccacgtgacc ggcgcacccc tacggatttg
     1621 ctgtctccgc caggctgtaa acaagaaagt gtttgccgcc gcgcgcggcc gccgcgcgat
     1681 ttttccattt cgagtcgcat tcattggccg gatttggtcg gattcagtcg gatttgactt
     1741 gtgggtcgga aggttaggtg ccgttgatcg ggaggtcttt cactcccgca ttcacgacga
     1801 caccgagcgt tcgccacgaa gaacttccga atctccgcag cgcgctcgga attcaaggga
     1861 cttgcaaccg ccctgcaaaa ggggacgcat cgcgaagggc aaaggttcgc aacccgtcca
     1921 agtgaagatc tgcgttcgac ctagggcccg accgctggaa agaactgttc gtgggtgatc
     1981 ttcccgtctt tgacggtata aacgccaacc tccgacatct gcatccgatt tttcgagttt
     2041 ttgtcggtca cgtcgagatc gaacgggacc acaaatcgat catgcgacac gaatggttcg
     2101 cccaccttca cgccatgcac ctccatatta gtcttccacc attccacctt ctcgcgcacg
     2161 ccttggatcc cacgcgtttc gggcgaacta ccgtccatcg tgcgcgcttc cacgctcaca
     2221 acgtcgtccg cgtagagtag ctcgatcgct tcattccagg cctgtttgcg gcacaactcc
     2281 accacttttt tcgcgacttc ttctgtattc atgccttgcc ttctgctcgg attcacgtcg
     2341 cgtcaatcaa cgattctgtg acaatgcgca ggagcttggt tcgctccgtc gaatgatgcc
     2401 tgcctcacca atttcccttc gctcgctttg tctgtggaga ttggtgctcg aaaactggca
     2461 aatgcctacg gcacgaccgt tagcgtgagg tcgtccccgt cgccgcctgc gtagctctct
     2521 tggaaggtgt tccgttggcg gtgaaaatca cttgggcaga gagcgttcag caagggtctg
     2581 ggatcatcat ggaatgagca gcactttccc gatgctttgg cggctctcga gataggcgtg
     2641 tgccttcgct ccatcgcgca ggggaaattc ttccgcgatt cgcaccttca atttcccggc
     2701 acgcacccac ccaaacaact ccgcggcgcg agcgatccgc tcgccgcggc tggtcagcac
     2761 gttccagaga tcaccgccgg tcagagtctt cgactcgtcc atcagccggc gcggatcgac
     2821 cggcgcagga tcgccgcctg tcatgccgta gaaaacgacc tgcccgcctt tgtgtgtaac
     2881 cgtgagactc gcatcgagcg tcgcgccgac ggagtcatag acaacatcgg ctcccttgcg
     2941 atc
//
LOCUS       DATMNS010000301         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3548138, whole genome shotgun sequence.
ACCESSION   DATMNS010000301 DATMNS010000000
VERSION     DATMNS010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3548138"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="VNW28_10700"
     CDS             complement(<1..1480)
                     /locus_tag="VNW28_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXA10442.1"
                     /translation="MSPSAEPRPAGIRRFRTKLTLAMMLVVSALTSLGLFVAQRRAAE
                     YAQTDLQRDFEIELASLHRLQDLRNAAVADRSIALASDARIHAALEDNALDLLYPSAR
                     AELRDLMEPNESGQVDAADRLHARFYRFLDAQGGVITPPTDDGGDLLPNEEAQLAQDR
                     TPQTMQTGYLARRDKTGHSTIDEMIAVPIRSTETNEVIAALVLGFKPVEAIVHGAASG
                     MKSGILVDGKLRLPAFDRATEEQISGEVKRDVAQADGQQHSLQTKIGGVSHLLFFKQL
                     NLNSSYPPAYEVCVYPLTQLLARQRQLRSQFLWAGGVLLLGGFLVSLFFSRRLSEPVE
                     ELAIVSEENFAQRQRAEAELQTTAEELQRSTRFSADASHQLKSPITVLRAGLDSLLAR
                     DGFPREVYEEISSLIHQTYRLTGVVEDLLLLSRLDAGKLRLNLHAVNLAHLVAEWIED
                     LQAMPDGSDLRVETDILANLEIAGETKFTALVVQNLLDNARKY"
     gene            complement(1477..2121)
                     /locus_tag="VNW28_10705"
     CDS             complement(1477..2121)
                     /locus_tag="VNW28_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXA10443.1"
                     /translation="MNGDLLLRLLAALGLVPAVCLGQATIEGHVELPKSHFVPVVSER
                     YEIVSRAGVLATEPPRAVVYLEGDFPKPTALPTKQVAQKNYAFLPPLLAIEVGTKVEF
                     PNLDDTYHNVFSYSPAKRFDLGRYPPGENPPPFQIFDKAGLVTLRCDIHEHMRGLILV
                     LASPYFAITDPDGHYRLTSLPKGHFTVKAWVDSKTTRERPVDLKNGTVQHVDLP"
BASE COUNT          427 a          633 c          685 g          421 t
ORIGIN      
        1 tatatttccg ggcgttatcg agcagatttt gcacgaccaa cgcggtgaat ttcgtctccc
       61 cggcgatctc taaattcgcc aggatatcgg tttcgacgcg aaggtcagat ccatccggca
      121 tggcctggag atcctcgatc cattcggcca cgaggtgagc caggttcacg gcgtgaagat
      181 tgagccgcag ctttccggca tccaaccgcg agagaaggag caggtcctcg accacaccgg
      241 tgagccggta ggtctggtga atgagcgatg agatttcctc gtagacttcg cgtggaaaac
      301 catcgcgcgc gagcaaggaa tcgagcccgg cgcgcaggac cgtgatcggg ctttttaact
      361 ggtgggaagc gtcggcggaa aagcgggtcg agcgttgtaa ttcttcggcc gtggtttgca
      421 gctcggcctc ggcccgttgc cgctgggcga agttttcttc cgagacgatc gccagctctt
      481 ccaccggctc cgacaggcgc cgcgagaaaa acagactgac caggaaaccg ccgagcagca
      541 ggacgccgcc ggcccagaga aattgcgagc gcaattgccg ctggcgggcg agcaattggg
      601 taagtggata aacgcagacc tcataggctg gaggataact cgagttgaga ttgagctgtt
      661 taaaaaaaag aaggtgcgac acgcccccaa tctttgtttg caggctgtgt tgctggccgt
      721 cggcttgggc gacatctcgt ttcacttcgc cggagatttg ttcctcggtc gcgcggtcga
      781 aggccgggag ccgcaatttt ccgtcgacca gaataccgct cttcatcccg ctcgccgccc
      841 cgtgaacgat cgcttcgacc ggcttgaacc cgagcacgag cgccgcgata acttcattcg
      901 tctcggtgga acgaatcggg accgcgatca tttcgtcgat cgttgagtgg cctgttttgt
      961 cgcgccgggc gaggtagccg gtttgcatcg tctgcggcgt ccggtcctgg gcaagctgcg
     1021 cctcttcgtt aggcaagagg tcgccgccat cgtccgtcgg cggggtgatg actccgccct
     1081 gcgcatcgag gaagcgataa aatctcgcgt gcagcctatc cgccgcgtcc acctggcccg
     1141 actcgttggg ttccatcaga tcacgcaact ccgcccgcgc gctggggtag agaaggtcga
     1201 gcgcgttgtc ctcgagcgcc gcatgaatgc gcgcatcgct ggcgagcgcg atggaacggt
     1261 cggccaccgc ggcgttgcgc aaatcctgga ggcgatggag cgaggcgagc tcgatttcaa
     1321 agtcccgctg taaatcggtt tgcgcatatt ccgccgcccg gcgttgcgcg acgaaaaggc
     1381 ctaacgacgt cagagctgag accaccagca tcatggcgag cgtaagcttg gttcggaaac
     1441 ggcgaatgcc agcggggcga ggctcggcgg acgggctcat gggagatcga cgtgctggac
     1501 cgtgccattc ttcaaatcga ccggacgttc ccgcgtcgtc ttgctgtcca cccaggcttt
     1561 gaccgtgaaa tgccccttcg gcaggctcgt taggcgatag tgcccgtccg ggtcagtgat
     1621 cgcgaagtag ggtgaggcga gcaccagaat caggccccgc atgtgttcgt ggatgtcgca
     1681 gcgcagcgtg accaggcccg ccttgtcgaa gatctgaaag gggggcggat tctcccccgg
     1741 cggataacga ccgaggtcga atcgtttcgc cggcgaatag gaaaaaacgt tatgataagt
     1801 gtcgtcgaga ttcgggaact cgactttcgt cccgacctcg atggcaagga gcggaggcag
     1861 gaacgcgtag tttttttgcg ccacctgttt ggtcggcaag gccgtcggct ttgggaaatc
     1921 gccttccagg taaaccaccg cccgtggcgg ctcggtcgcg agcacgcctg ccctgctcac
     1981 gatctcgtag cgctcactga ccaccggcac gaaatgcgat ttcggcagct ccacgtggcc
     2041 ctcgatcgtc gcctgtccaa ggcaaacagc cggcaccagg ccgagcgcgg cgaggagtcg
     2101 gaggagcaaa tcaccattca tttcttctca tcataacagg atcgaggccc gccgaaacgg
     2161 aggaaa
//
LOCUS       DATMNS010000302         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3186667, whole genome shotgun sequence.
ACCESSION   DATMNS010000302 DATMNS010000000
VERSION     DATMNS010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3186667"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VNW28_10710"
     CDS             complement(<1..415)
                     /locus_tag="VNW28_10710"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXA10444.1"
                     /translation="MDKLVIRGGEPLLGTIRTSGAKNAALPCMAAALLTDEPVILENI
                     PQVRDIETTRKLLAAMGAEVELGFGRAQHRTTICAKTLNAPEASYELVKTMRASTLVL
                     GPLVTRCGRARVSLPGGCAIGARPIDLHIKGLEKLG"
     gene            complement(518..1438)
                     /gene="murQ"
                     /locus_tag="VNW28_10715"
     CDS             complement(518..1438)
                     /gene="murQ"
                     /locus_tag="VNW28_10715"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF009222.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HXA10445.1"
                     /translation="MKHPDLRRLTTEKSNPRSANLDRKSSLEIARIINAEDTKVAAAV
                     KRALPQIAQAIDLIADSISRGGRLIYVGTGTSGRIAALDAAEIPPTFDTNPRTVQFII
                     AGGTKALAKATESSEDSRALGEKEMAKKKPGKNDVVVGVTASGRTPFTIAALRYARAR
                     GSKTVAVTCNRNSELAKAAHIEIVAEVGPEVVTGSSRMKAGSAEKMILNMLSTGAMTR
                     LGYVYGNLMVHLRLKNSKLVERGIGIVEAATGLNRSQAVKLLDSARNSVATAIVMAKA
                     NVKRPIAEAALKKSKGQVRKAIEIAKRKPA"
     gene            complement(1435..2607)
                     /locus_tag="VNW28_10720"
     CDS             complement(1435..2607)
                     /locus_tag="VNW28_10720"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXA10446.1"
                     /translation="MRNTSEQEEKFRSAFAILEKTVAARAFPGCALAVTLRGELVAHY
                     AVGRFTYDPASPVVTTASIFDLASVTKAVATTTMAMILYERGLLDLEAPVAAILPEFV
                     GRSNHPSRREVTLRMLLAHSSGLPAYEKLFLKNTTREEVLAAALTTHLTAEPGTRAEY
                     SDIGFIILGVALERLADEPLDTFCQREIFGPLGMSHTTYNPPKSWRDKTVPTADETSA
                     EKCSAGAPTREPSTAPATSSAGKFRNRVIQGEVQDENASILGGVAGHAGLFAPVEDLA
                     IFAHAMLTGGRPILRPETIDLFTRRESQPEGTSRALGWDTPSSPSQSGKYFGSRSYGH
                     LGYTGTSLWIDPDRQLSITLLTNRTWPDCQSQAIKQVRPAFHDAVIEALKSQGLIP"
     gene            2788..>3270
                     /locus_tag="VNW28_10725"
     CDS             2788..>3270
                     /locus_tag="VNW28_10725"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXA10447.1"
                     /translation="MHRPVKIFFLSVVACCALAGAQVSRDAQLSVLDPIVNNAIAQGQ
                     VPGAVLIVGHEGRVVYRKAYGNRAIDPRREAMTLTTVFDCASLTKVISTTTAMMQLWE
                     RGKFRMADPVAKYLPEFAQNGKQDITIRQLMIHYSGLAPDLDLTKPFAGKEAGYRMAF
                     A"
BASE COUNT          540 a          808 c         1180 g          742 t
ORIGIN      
        1 cgccgagctt ttcgagtccc ttgatatgga gatcaatcgg gcgggcgccg atagcgcacc
       61 cgccgggaag ggagactcgg gctcgtccgc aacgggttac cagcggtccg aggaccagcg
      121 tggaggcgcg catcgtcttc accaattcgt aggacgcttc cggcgcgttg agcgttttcg
      181 cgcagatggt cgtgcggtgc tgggcgcgtc cgaagccgag ttcgacttcg gcgcccatgg
      241 cggccaggag tttgcgcgtg gtctcgatgt cgcgaacttg cgggatgttc tccaggatga
      301 cgggttcgtc ggtgagcagg gcggctgcca tgcaaggaag cgcggcgttc ttcgcgccgc
      361 tggtgcggat ggtgcccagt aacggttcgc cgcctcggat gactagcttg tccattggtt
      421 tcttcattct agcgggacgg gttccgcatt gcgattgttt tgctccattt acaccgcaag
      481 tcgaaggctt gaccacgggg gacacggagg tcaagggtta tgctggttta cgtttcgcga
      541 tttctatggc tttgcggacc tggcctttgg attttttcag ggcggcttcc gcaatggggc
      601 gtttcacgtt tgctttcgcc atgacgatcg cggtcgctac tgagttccgg gcagagtcta
      661 gtagtttgac tgcctggctg cgattcaagc cggtggcggc ttctacaatg ccgattccgc
      721 gctcgaccag tttcgaattc ttcagacgga gatggaccat caagttgcca tagacgtatc
      781 cgagacgcgt catcgcgccc gtcgataaca tgttcaggat catcttttcg gcggagccag
      841 ctttcatgcg gctcgatccg gtgactactt cagggccgac ttcggcgacg atctcgatgt
      901 gcgcggcttt tgctagctct gaatttcgat tgcaggtgac agccacggtt ttcgatcctc
      961 gggcgcgcgc gtaccgaagg gcggcgatgg tgaagggcgt gcgtccgctg gcggtaacgc
     1021 caactactac atcgttcttg cccggcttct tttttgccat ttctttttcg ccgagtgcgc
     1081 gcgagtcttc gctggactcg gtggctttgg ccagcgcttt ggttccgccg gcgatgatga
     1141 actggacggt gcgcgggttg gtgtcgaagg tgggcgggat ttcagcggcg tcgagagcgg
     1201 cgatacggcc gctggtgccg gtgccgacgt agatgaggcg tccgccgcgg gagatggagt
     1261 cggcaatcag gtcgatggct tgcgcaattt gcgggagggc gcgctttacg gcggctgcta
     1321 ctttggtgtc ttcggcgttg atgatgcggg cgatttcgag cgaggacttg cggtcgaggt
     1381 tggcggaacg gggattggat ttttcggttg tgaggcggcg gaggtcgggg tgcttcatgg
     1441 gatgagtccc tgggatttca gggcttctat cacggcatcg tgaaatgccg ggcgcacttg
     1501 tttgatcgcc tgactttggc aatcgggcca tgtgcggttg gtcagcagtg tgatggagag
     1561 ctggcggtca gggtcgatcc agagggaggt tccggtgtac ccgagatggc cgtaggagcg
     1621 ggagccgaaa tattttccgg attgagatgg agatgaaggt gtgtcccatc cgagtgcgcg
     1681 ggatgtgcct tcgggctgcg attcccggcg cgtaaaaagg tcgatggttt cggggcggag
     1741 gatggggcgt ccgccggtca acatggcgtg agcgaagata gcgaggtctt cgacgggggc
     1801 gaagagacca gcgtgtccgg cgactccgcc gaggatgctg gcgttttcat cctgaacttc
     1861 gccctggatg actcggttgc gaaacttgcc ggcagacgac gttgctggag cagtcgacgg
     1921 ttcgcgggtg ggggcgcccg cgctacattt ttcggcggag gtttcgtcgg cggtggggac
     1981 ggtcttgtcg cgccacgatt ttggcggatt gtatgtggtg tgcgacatgc cgagagggcc
     2041 gaagatttcg cgttggcaga aggtgtcgag gggttcgtcg gcgaggcgtt ctaaggcgac
     2101 tccgaggatg ataaagccga tgtcgctgta ttcggcgcgc gtaccgggtt cagcggtgag
     2161 gtgggttgtg agagccgcgg ccagcacttc ttcccgcgtt gtgtttttca ggaagagttt
     2221 ctcgtaggcg ggcaggccgg aggaatgagc cagcaacata cgcagagtta cttcgcggcg
     2281 gctcgggtgg ttactgcgtc cgacgaattc cgggaggatg gcggcgacgg gcgcttcgag
     2341 gtcgagcagt ccgcgctcgt agaggatcat cgccatggtg gtggtggcta cggcctttgt
     2401 gaccgatgcc aggtcgaaga tgctggcggt ggtgacgaca ggcgaagcgg gatcgtaggt
     2461 gaagcggcca acggcataat gggcgacgag ttctccgcgc agggtcacgg ctaatgcgca
     2521 gccggggaag gcgcgggccg cgacggtttt ttcgaggatc gcaaatgcgc tacggaattt
     2581 ctcctcttgc tcgctggtgt tgcgcaccag gaaggcgaag ccggcgggcg gattgtgggt
     2641 cgcagtcaag gcgttggggc tgatcgggtt atagcaaacg cggcggagcg aggcaaacgg
     2701 cggtagcacg gggagttact aacggggacg cggctggcta ccgattgctt gaggcgtctg
     2761 atctgggtcg tgtagagtca cagcagagtg catcgtcctg taaaaatctt tttcctgagt
     2821 gtcgtggcgt gctgtgcgct ggccggcgcg caggtttccc gcgacgccca gttgagcgtt
     2881 ctcgatccca ttgttaacaa cgcgattgcg caggggcagg tcccgggcgc ggtgctgatc
     2941 gtcggacatg aagggcgggt cgtttatcgc aaggcatatg ggaatcgcgc gatcgatcct
     3001 cgccgcgaag cgatgacgct gactacagtg tttgactgcg cttcgctcac aaaggtgatc
     3061 tcaactacca cggcgatgat gcagttgtgg gagcgcggga agttccggat ggcggatccg
     3121 gtggcgaagt atctgcccga gtttgcgcag aatgggaagc aggacatcac gatccggcag
     3181 ttgatgattc attattctgg actggcgccg gatttggatc tgacgaagcc ctttgcggga
     3241 aaggaggcgg gttaccggat ggcatttgcc
//
LOCUS       DATMNS010000303         7691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3555235, whole genome shotgun sequence.
ACCESSION   DATMNS010000303 DATMNS010000000
VERSION     DATMNS010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 7691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7691
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555235"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1650)
                     /gene="fusA"
                     /locus_tag="VNW28_10730"
     CDS             complement(<1..1650)
                     /gene="fusA"
                     /locus_tag="VNW28_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXA10448.1"
                     /translation="MTNPNSPNRKFPLERTRNIGIAAHIDAGKTTLTERILFYTGMIH
                     KIGEVHEGTTVTDWMEQERERGITITSAATTCAWTQKPEPGVYKMFEDVKMRVNIIDT
                     PGHVDFTAEVERSLRVLDGAIAVFDAVAGVQPQSETVWRQANKYLVPRIAFINKMDRV
                     GADFEMSIESMRKKLGANAWPILIPLGKEDYLKGQLDVVNRKAVFYLDDDSMGSIYEV
                     RDLPEEHKAKVEQAYADLVEAISNIDDEVAEAVLEEKPVTPEMLKAGIRRQTIANKFV
                     PVVGGSAFKNKGVQYLVDAVIDYLPSPLDIPPAVGMDPDSHEKMEAPTDDHGKFCSLA
                     FKLWSDPFVGKLVFFRVYSGSLTKGDTVYNPRTNKRERISRLIQIQADKREDIDTCYS
                     GDIAAIVGIKNITTGDTLCDEDFPILLEPPSFPDPVISMAIEPKTKQDQEKMGVALQR
                     LSEEDPTFKVYTHEDTGQTIIAGMGELHLEIIRDRMLREFKVDANAGKPQIAYRETIT
                     AAADGEGKLIKQSGGRGQYGHVIIKVQPNERGKGITIENKVV"
     gene            complement(1851..2324)
                     /gene="rpsG"
                     /locus_tag="VNW28_10735"
     CDS             complement(1851..2324)
                     /gene="rpsG"
                     /locus_tag="VNW28_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007526570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXA10449.1"
                     /translation="MSRRRRTYKKEEKVDSRYGSVAVARLISTVMKRGKKSLAERIVY
                     TAIDKSREGSDAVDPLEVVNKALENVRPRLEVKSRRVGGATYQVPMEVTASRQVSLAM
                     RWIVTYADNRRGMPMADALAHELRDAAAGQGNAIKKRDDTHKMAQANRAFAHFRW"
     gene            complement(2362..2775)
                     /gene="rpsL"
                     /locus_tag="VNW28_10740"
     CDS             complement(2362..2775)
                     /gene="rpsL"
                     /locus_tag="VNW28_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXA10450.1"
                     /translation="MPTINQLIRKGRKKVSVKSKSPALKNCPQRRGVCTQVMTRTPKK
                     PNSALRKVAKVRLTNGQEIIAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYHIVRGTL
                     DSLGVDGRRRSRSKYGAKRPKAAGEAGAEKGAKKK"
     gene            complement(2906..4804)
                     /locus_tag="VNW28_10745"
     CDS             complement(2906..4804)
                     /locus_tag="VNW28_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXA10451.1"
                     /translation="MSELVLNTIASARELVVRFGNQVVLDRASVTILEGERVGLVGRN
                     GSGKSTFLQIAAGVMPPDAGELNRRRDLVVGYMPQLFELDETETVHANILAGAQQVLD
                     LIHEYENAPPASARSGLLLDQIGHFDGWNLEHRIKSLVTNLHAPGPERAVSTLSGGEK
                     RRVALCRALLARPDFLILDEPTNHLDTGSIEWLEDFLARYSGTCLFVTHDRYFLDRIA
                     TRVVELAGGKFYSYAGNYTDYLLARAERQAVEEMQEDKRQKFLKRELAWVRKAPRARR
                     TKSVDRVERYFEVAAQEAPEAELDVDLIIPPAPKLANRVIELREVGMVLGGRSLIENL
                     SLNLAPGERLGIVGRNGLGKTTLLKSMLGESEPTRGVVEIGARTEINYVDQNRLLLDD
                     AKTVWEEVGEGSEFVRLGEENITLRGYLRRFLFTEERINTKIDQLSGGERSRVLLAKI
                     LKRGGNVLILDEPTNDLDLGTLRLLEEALIAFGGDVIVVSHDRYFLNRVCTSILAFEG
                     DGCIRYHVGNYDYYLEKRATVGAENADPGPSERRKPAGISDPGAKRKPRKLKWTEARE
                     LEGMEAAILAAEKEVVRLETLFAAPDFYATHAADWQKLEADLDAARHRVTELYARWEE
                     LGAIAAPK"
     gene            4877..6022
                     /gene="tgt"
                     /locus_tag="VNW28_10750"
     CDS             4877..6022
                     /gene="tgt"
                     /locus_tag="VNW28_10750"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXA10452.1"
                     /translation="MFELFTTDPGSKARRGRFTTAHGVIETPAFMPVGTQGSVKGVSP
                     RELHELDAQIILGNTYHLFVRPGLEVIRHFGGLHRFMNWDGPILTDSGGYQIFSLAKL
                     RKITEEGVQFQNHLDGTPTFISPESAMEIQAALGSDIAMVLDECPPYPCEHEYAVRSL
                     EMTARWAQRCKDANVQRPTSNVQRSTEEGTRRLLFGIVQGGTFEDLRRASAAATVQIG
                     FDGYAIGGVSVGEPEPEMMQAVEWSEPLLPNDKPRYAMGLGTPPQLLELIARGMDMFD
                     CVLPTRLARNGTAFTAGGTLNLKNAEFTLQKGPIEEGCVCPACSGFSRSYLRHLIKAE
                     EILGLRLLTLHNLHFYLNLMKRVRSEIEAGRFAAFRRDFVAGYRTNN"
     gene            complement(6070..6951)
                     /locus_tag="VNW28_10755"
     CDS             complement(6070..6951)
                     /locus_tag="VNW28_10755"
                     /inference="COORDINATES: protein motif:HMM:NF025273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-like domain-containing protein"
                     /protein_id="HXA10453.1"
                     /translation="MRLFPLLLVAGLIVTATAGPSPLSNKEIALMLRSGYSSDAVLAE
                     ITSRRVLEAFDPAMQKSLVDFGANPQLISALASNAYLVSAAEADRAKQREAELSAQRA
                     AEIERSQKLSTIYQAGQAEARARTAAAPAAGMPMLDALRQKLVRCHDGTVSRAIGTEL
                     DNKKFVALYYSAHWCAPCRKFTPQLVDYYNRVVVEHPEFELIFVSADRSRFGWETYIQ
                     EAKMPWLAIDYDQLADLPGLKQLGGTGIPSLLILDASSHIVASSYDGDKYLGPQNALA
                     ALDKIFATNAGPIAQAR"
     gene            complement(6909..7448)
                     /locus_tag="VNW28_10760"
                     /pseudo
     CDS             complement(6909..7448)
                     /locus_tag="VNW28_10760"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7481..>7691
                     /locus_tag="VNW28_10765"
     CDS             7481..>7691
                     /locus_tag="VNW28_10765"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HXA10454.1"
                     /translation="MIELIVPNEQAGLRLDRYLALALPQFSRSRLQNLIRTGDVRLQG
                     KAARTRETVHSGDVVRLTVPPLEEID"
BASE COUNT         1423 a         2340 c         2284 g         1644 t
ORIGIN      
        1 cacgaccttg ttctcaatcg tgatgccttt gccgcgctcg ttaggctgaa ccttaatgat
       61 gacgtggccg tattgaccgc gcccgcccga ctgtttgatc aacttgcctt cgccatcggc
      121 cgctgccgtg atggtttcgc ggtaagcgat ctgcggcttg cccgcattcg cgtccacttt
      181 gaactcgcgc agcatgcgat cgcggatgat ctcgagatgc agctcgccca tcccggcgat
      241 aatcgtctgc ccggtatcct catgggtgta aaccttgaag gttggatctt cctcggagag
      301 ccgctgcagg gccacgccca tcttctcctg gtcctgcttg gtcttcggct cgatcgccat
      361 cgagataacc ggatcaggga aagaaggcgg ttcgagcagg atcggaaaat cctcgtcgca
      421 cagcgtgtcg cctgtcgtga tgttctttat cccgacgatt gcggcgatgt cgcccgagta
      481 gcaggtatcg atgtcttcac gcttgtcggc ttgaatctga atgagacgcg agatgcgttc
      541 gcgtttattg gttcgcggat tgtaaaccgt gtcgcccttc gtgagggaac cagagtagac
      601 gcggaagaaa accaacttcc cgacgaaggg atcgctccac aatttgaaag cgagcgagca
      661 gaatttgccg tggtcatccg tcggcgcttc catcttctcg tgcgagtcgg gatccatgcc
      721 gaccgccggc gggatgtcga gcggactggg cagataatca atcacggcat cgacgagata
      781 ttgcacgccc ttgttcttga aggcggagcc gcctacgacc gggacgaatt tattcgcgat
      841 cgtctgccgg cgaatgcccg ccttcagcat ttccggcgtg accggctttt cctccagaac
      901 ggcctccgcc acttcgtcgt cgatgttgga aatcgcctcg accagatcgg cataagcttg
      961 ctcgaccttg gccttgtgct cttccggcag gtcgcgcact tcgtagatgg aacccatcga
     1021 gtcgtcatcg agatagaaga ccgccttgcg gttcacgacg tcgagttggc ctttgagata
     1081 atcttctttg cctaacggaa ttaggatcgg ccaggcattt gcgccgagct tcttgcgcat
     1141 gctctcgatc gacatctcga agtcggcgcc gacgcggtcc atcttgttga tgaaagcgat
     1201 gcgcgggaca agatatttgt tcgcctgccg ccagaccgtc tcggattgcg gttgcacgcc
     1261 ggcgaccgcg tcgaagacgg caatcgcgcc gtcgagcacg cggagcgaac gctccacctc
     1321 ggcggtaaaa tccacgtgac cgggcgtgtc gatgatgttg acgcgcattt tgacgtcctc
     1381 aaacatcttg taaacgccgg gctccggctt ttgggtccag gcgcaggtcg tggccgccga
     1441 ggtgatcgtg atgccgcgct cgcgttcctg ctccatccag tcggtcacgg tcgtgccttc
     1501 atgcacttca ccgatcttgt ggatcatgcc ggtgtaaaaa agaatgcgct ccgtcagcgt
     1561 cgtcttgccg gcatcgatgt gcgcagcgat cccgatgttc cgcgtccttt cgagcgggaa
     1621 cttgcggttc ggcgaattcg gattcgtcat tgcaggtttt tctaaagtcg cggacgccat
     1681 ggctagggaa aattcgaaat ccgaaatccg aagttcgaaa caaattcaaa acccgaaaag
     1741 cgaatttccg aaacggactg gagcgcacac cgtttcgata tttggtgatt agtcattacc
     1801 agattgtttc gggtttcgat attcgggttt cgtcattccg cgccgcggcc ttaccagcgg
     1861 aaatgggcaa aggcgcggtt ggcctgcgcc attttgtgcg tgtcgtcgcg ttttttgatc
     1921 gcgttgccct gaccagcggc cgcgtcgcgc agctcgtgcg ccagggcatc ggccatcggc
     1981 atgccgcgcc gattgtcggc gtaggtcacg atccagcgca tcgcgagcga gacctgccgg
     2041 gaagccgtga cttccatcgg gacttgatag gtggcaccgc cgacgcgacg cgatttcact
     2101 tcgagacgcg ggcgcacgtt ctcgagcgct ttgttcacta cttcgagcgg atcaaccgcg
     2161 tcggaacctt cgcgcgactt gtcgatcgcg gtataaacga tccgctcggc gagcgatttc
     2221 ttgccgcgtt tcatgacggt ggaaatcagc cgggccaccg cgacgctgcc gtaacgggag
     2281 tcgacctttt cttctttttt gtaggttcta cgacgacgag acattggaag ggtttagagt
     2341 tcttggctga gagctgagag attatttctt tttcgcgccc ttctcggccc cggcctcacc
     2401 ggcggctttt gggcgtttcg cgccgtattt ggaacggctc cggcggcggc catccacccc
     2461 taacgagtcg agcgtcccgc ggacaatatg gtaacgcacg cccggcaaat ccttgacccg
     2521 tccgccgcgc acgaggacga tcgagtgttc ctgcaaattg tggccttccc ccggaatata
     2581 ggcgatgatt tcctgcccgt tcgttaggcg cactttcgcc actttgcgca gggcggaatt
     2641 cggcttcttc ggcgtccgcg tcatcacttg cgtacagaca ccgcgccgct gcggacaatt
     2701 cttcagggcc ggtgattttg atttcaccga aacctttttt cgtcccttgc gaataagctg
     2761 attaatcgtt ggcatttttg tagattgcag ccgcgaaacc gtgaagtttc aggcgacaaa
     2821 aaaaccgcct caagccgtgt ccaccgtcct cacgcacctg atacggagag cgcgcaatat
     2881 aggagcaaat tattgggtcg caagattatt ttggcgcggc gatggcgccg agctcctccc
     2941 agcgcgcgta aagttcggtg actcggtgcc gcgcggcgtc caggtcggcc tccaactttt
     3001 gccagtcggc ggcgtgggtc gcgtaaaaat ccggggcggc aaagagcgtt tccagcctaa
     3061 cgacctcttt ttcggcggcg agaatggcgg cttccatccc ttccagttcg cgtgcctctg
     3121 tccacttcaa tttccgcggt ttccgtttgg cgccgggatc gctgatcccg gccggcttgc
     3181 gtcgctctga ggggccggga tcggcgttct ccgctccaac agttgctcgt ttttcgaggt
     3241 agtagtcgta gttgcccacg tgataccgga tgcagccgtc gccctcgaag gcgagaatcg
     3301 aggtgcagac gcgattcaga aaatagcggt cgtgactgac gacaatcacg tccccgccaa
     3361 aggcgatgag cgcttcctcg agcaaccgca gggtgccgag atcgagatcg ttcgtcggct
     3421 cgtcgaggat gaggacgttg ccgccgcgct tgagaatttt cgcgagcaaa acgcggctcc
     3481 gttcgccacc gctcaattgg tcaatcttgg tgttgatccg ttcttcggtg aaaagaaaac
     3541 gtcgcagata gccgcggagg gtgatgtttt cttcgcccag cctaacgaac tcgctccctt
     3601 cgcccacttc ttcccagacc gtcttcgcat cgtcgagcag gaggcggttt tgatcgacat
     3661 aattgatttc ggtgcgcgcg ccgatctcga ccacaccacg cgtcggctca ctttctccga
     3721 gcatgctttt gaggagcgtg gttttcccga gaccgttgcg cccgacgatt ccgagccgct
     3781 cgcctggcgc gagattgagc gagagattct cgatcagcga tcgcccgccg agcaccattc
     3841 cgacttcgcg cagttcgatc acgcgattcg ccagcttcgg cgcgggcgga atgatgagat
     3901 caacgtcgag ttccgcttcc ggcgcttcct gcgccgccac ctcgaaatag cgctcgaccc
     3961 gatcgaccga tttcgtccgc cgcgcgcgcg gcgctttgcg aacccaggcc agctcacgct
     4021 tcagaaattt ctgacgcttg tcctcctgca tctcctcgac cgcctgccgc tcggcgcgcg
     4081 ccaacaggta atcggtgtaa ttgccggcgt agctgtagaa tttcccaccc gccagctcga
     4141 ccacgcgcgt cgcgatgcga tcgagaaaat agcggtcgtg ggtcacaaac agacaggtgc
     4201 cgctgtaacg cgcgagaaaa tcctctagcc actcgatcga gccggtatcg agatggttcg
     4261 tcggttcgtc gagaatcagg aaatccggtc gggcgaggag cgcccgacag agcgcgacgc
     4321 gccgtttctc gccgcccgac aatgtcgaga ctgcgcgctc cgggccgggc gcgtgcaggt
     4381 tggtgaccag actcttgatc cgatgctcga ggttccaccc gtcgaaatgt ccgatctggt
     4441 cgagcagcag gccgctccgc gcgctcgcgg gtggcgcgtt ctcgtattcg tgaatgagat
     4501 ccagaacctg ctgcgcaccg gccagaatat tggcgtggac cgtctcggtc tcatcaagtt
     4561 caaacagctg cggcatgtaa ccgacgacga gatcacgccg ccgattcagc tcgcccgcat
     4621 cgggcggcat gacacccgcc gcgatttgca aaaatgtcga tttgccggag ccgttgcggc
     4681 cgaccagacc gacgcgttcc ccttccagaa tcgtgaccga cgcgcgatcg agcaccacct
     4741 ggttgccgaa gcgcacgacg agttccctcg cgctcgcgat cgtattcagg acaagctcgg
     4801 acatccgatg acatagcata cggaaggcag gtcttcccgc ttgccagcgc cgcccgctta
     4861 ccgaacgtag gcgcgaatgt tcgaactctt caccaccgat cccggctcaa aggcgcgccg
     4921 cggccgcttc acgacggcgc atggcgtgat cgagacgccg gcttttatgc cggtcggcac
     4981 ccagggtagc gtgaagggcg tcagtccccg cgaactgcat gaactcgacg cccagattat
     5041 tctcggtaat acctatcacc ttttcgttag gccaggactc gaggtcatcc ggcattttgg
     5101 cggcctgcat cgcttcatga attgggacgg gccgatcctg accgacagcg gcggctatca
     5161 gattttctcg ctcgcgaagc tgcgcaagat cacggaggag ggcgtccaat tccaaaatca
     5221 tctcgacggc acaccgactt ttatcagtcc ggaaagcgcg atggaaatcc aggccgcgct
     5281 gggaagcgat atcgcgatgg tgctggatga atgcccgcct tatccgtgcg agcatgagta
     5341 tgcagtgcgc agtttggaga tgaccgctcg ctgggcgcag cggtgcaagg acgcgaacgt
     5401 ccaacgtcca acgtccaacg ttcaacgttc aactgaagag ggaacgcgcc ggctcctttt
     5461 cgggattgtg cagggcggta ccttcgaaga tctgcgccgg gcgagtgcgg cggcgacggt
     5521 gcagatcggc ttcgacggct atgcgattgg cggggtgagt gtgggcgagc cggagccgga
     5581 gatgatgcag gccgtggaat ggagcgagcc tctcttgcct aacgacaagc cgcgttacgc
     5641 gatgggcctc ggcaccccgc cgcagttgct cgagctaatc gcccgcggca tggatatgtt
     5701 cgattgcgtg ctgccgacgc gtctggcccg caatgggacc gccttcaccg ccggaggcac
     5761 gctcaacctg aagaatgccg agttcaccct gcagaagggc ccgatcgaag agggctgtgt
     5821 ctgcccggcc tgctccgggt tttctcggag ttacctgaga cacttgatca aggccgaaga
     5881 aatcctcggc ctgcgcctgc tgactctgca taacctgcac ttctatttga atctgatgaa
     5941 gcgtgtccgt tctgagattg aggccggccg cttcgccgcc ttccggcgcg attttgtcgc
     6001 cggatatcgg accaataact gatcgcgtct ggctgccgca cttgagtccc cggcaaggat
     6061 tggcggtttc tatcgtgctt gtgcaatcgg acctgcgttc gtcgcgaaaa ttttgtcgag
     6121 tgcggcgagc gcattttgcg gtcccaggta tttgtcgccg tcgtaactgc tcgcgacgat
     6181 gtgactgctc gcgtcgagga tgaggagcga aggaataccg gtccctccga gttgcttgag
     6241 tcccggcaga tcagcgagct ggtcgtaatc gatcgccagc cagggcatct tcgcttcctg
     6301 gatgtaggtt tcccagccaa agcgggaacg atccgcactc acgaaaatga gctcaaactc
     6361 cggatgctcc accacgacgc gattgtagta gtcgaccaac tggggtgtga acttgcggca
     6421 gggggcacac cagtgggcag agtagtaaag cgcgacgaat ttcttgttgt cgagctcggt
     6481 gccaatggcg cggctgaccg tgccgtcgtg gcagcggacg agcttttgcc tgagcgcgtc
     6541 cagcatgggc atccccgccg caggcgcggc ggcggtccgg gcgcgggctt ccgcctgccc
     6601 cgcctggtaa atagtgctga gcttttgact ccgctcgatc tccgccgcgc gctgcgcgct
     6661 cagctcagcc tcccgttgtt ttgcccggtc ggcttccgct gcggagacca gatacgcgtt
     6721 gctcgcaagc gcgctgatca gttgtggatt tgccccgaaa tcaaccagtg atttttgcat
     6781 cgcgggatca aacgcctcga gcacccggcg gctggtgatc tccgcgagga ccgcgtcgct
     6841 cgaataacct gagcggagca tgagcgcgat ctctttgttg gacaaaggag atgggcccgc
     6901 cgtcgccgtc acgatgagcc cggcgaccag gagtagagga aataggcgca tccctcctct
     6961 gaagcaaaag atacgccagc tacttcttca cgccgggaaa ggagcgctat ttcgctccca
     7021 cttcgtgcgt gccgggcggc gagcaaaatc aacgatcttc gcggtgcgcc gatccgcaaa
     7081 aagaatattc ccgggtttga aagcgcgatg aataagcccc gcctggaacg ccgccggcaa
     7141 gccctctccc gcagcttgcg ggccgacctc gagctctcca ttcccgaaat gcgcttttcg
     7201 tcttccatcc gatcgtcgag attgccgcta tcgaccagct ccatcatgcg atagaattgc
     7261 ccgtggtccc cgccgggcga gaaaacctcg atcacgtgcg ggtgcttgat cgacgcggtg
     7321 atgcgcgctt cctcctgcag ccacgcggtc aagatggcgt cgccggcaaa ctcctggcgc
     7381 aggagcttgc gcgcgacgaa gcgattcagc cgcgtgtcgc gcgcccagta aaccgaggcc
     7441 atcccgccgg tcccgagcgg ctctcaagcc ggagaatgcg atgatcgaat tgatcgtgcc
     7501 taacgagcag gccggcttgc gtctcgaccg ctacctggcc ctggctctcc cgcagttctc
     7561 ccgctcgcgc ctgcagaatc tgatccgcac gggcgacgtc cgcttgcagg gaaaagcagc
     7621 gcgcacccgc gagacggtgc actccggcga tgtcgttagg ctaacggttc caccactcga
     7681 ggaaatcgat g
//
LOCUS       DATMNS010000304         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3193563, whole genome shotgun sequence.
ACCESSION   DATMNS010000304 DATMNS010000000
VERSION     DATMNS010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3193563"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..848
                     /locus_tag="VNW28_10770"
     CDS             <1..848
                     /locus_tag="VNW28_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXA10455.1"
                     /translation="ESADFTAIYDIDAANAAQIAGQYKVRAATSLEEFSELIDAASVA
                     TPTNSHFAIARPLLEKGKHLLVEKPIAEDTAQASELAELAAQRHLVLQVGHVERFNPV
                     LSALEARLTHPRFIEAHRLSPFPNRSTDIGVVLDLMIHDLEIVLHLVRAPVQSIDAVG
                     VPVLSRREDIANARIRFENGCVANITSSRISPERMRKIRVFQEDAYLSLDYQNQTGEI
                     YRKIGGGIERAEVEIEKEEPLKRQILSFIECARTGVQPKVSGFEATAALELAVEITKR
                     IESAQ"
     gene            1503..2642
                     /gene="lpxB"
                     /locus_tag="VNW28_10775"
     CDS             1503..2642
                     /gene="lpxB"
                     /locus_tag="VNW28_10775"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXA10456.1"
                     /translation="MKIYFIAGEASGDNHGAALMRSLRLLRPDLQLSGRGGPQMKQIA
                     GSEFKDWIDQAGVVGLWEVVKHYGYFRQEFRKTLHEIAATAPNAVVLIDFPGFNLRLA
                     RALRGTRTLAGLGGKIIYYISPQVWAWNRRRIPQMARWLDLMLCIFPFEAGLYEKSGL
                     RTIFVGHPMIENLGAKRTDALRAPYLVGLFPGSRTREVRKLLPILVEVMRKLHVQRPE
                     LHFEIAAASEAIATAIRRDLQPVVNSLGQVAVVVGQTAQTMQRSAVGVVASGTATLEA
                     AFFRLPFVLVYKVAALTYLAGRMLIRVKHLGMPNVLADREIVAEFIQGKAQPPAIAAA
                     VLTLLDDRARRERMLADFDAVITKLGKGGANEAAARAILEAVNEK"
     gene            2639..3670
                     /locus_tag="VNW28_10780"
     CDS             2639..3670
                     /locus_tag="VNW28_10780"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HXA10457.1"
                     /translation="MNTIEERISRLHAGRLIDLHFDLPLILFLSRPRRNIIAPDLLPQ
                     FEAGDIGLLGVALYVADQYLGEPALRVVLDQVALLKAELETTPRLVLCRTFAEIEQAR
                     REGRIALLLTMEGAEPLGDDLNLLHIFHELGLRAISLTHARANAAAAGGIFAATGSPA
                     TGLTSFGREVVQECERLGIMLDLAHINPTGFEEICALTSKPLIVSHTNARHFYDIERN
                     ISDDQIKMIGARGGVIGINAILVSAKQEEATLDGYVDHIEHVANLIGIDGVAIGFDFC
                     ELLLGLLPAAERDELEAKLTKPHYLPDLSHHGQARNLTRKLIERGFSDEQIEKILRDN
                     WLRVLREFL"
BASE COUNT          781 a         1193 c         1191 g          762 t
ORIGIN      
        1 cggagtcagc cgatttcacc gcgatctatg acatcgacgc ggccaatgcc gcgcagattg
       61 cggggcaata caaggtccgg gcggcgactt cgctcgagga attttccgag ctgatcgacg
      121 ccgcctcggt cgctacgccg accaattccc atttcgcaat cgcgcgtcct ttgctggaaa
      181 aaggaaaaca tctgctggtg gagaagccaa tcgcggagga caccgcgcag gcgagcgaac
      241 tcgccgagct cgccgcgcag cgtcatctcg tgctccaggt cggccacgtt gaacgtttca
      301 atccggtgct cagtgcgctc gaggcgcgcc tgacccatcc gcgcttcatc gaagcgcatc
      361 ggctttcgcc gttcccaaat cgcagtaccg acatcggggt cgtactcgac ctcatgattc
      421 acgatctcga aattgttttg catctcgtta gggcgcctgt ccagtcgatc gatgccgtcg
      481 gcgtcccggt cttgagccgg cgcgaagaca tcgccaacgc gcgcatccgt ttcgagaacg
      541 gttgcgtggc caacatcacc tcgagccgca tcagtcccga acggatgcgc aagattcgtg
      601 tcttccagga ggacgcgtat ctctcactcg attatcaaaa ccagaccggc gaaatttatc
      661 gcaagatcgg cggcgggatc gagcgcgccg aggtcgagat cgaaaaggaa gaaccgctca
      721 agcgccagat cctttccttt atcgaatgcg cgcggaccgg ggtgcagccg aaggtttccg
      781 gtttcgaggc gaccgccgcc ctcgagttgg cggtcgagat cacgaaacgg attgagtcgg
      841 cccaatgagc gcgccgatgg aatcctcgga gggacgtgct tccgcacgtc cttccttggg
      901 tcccaaggcc gcgaaaccag agacgtgcgg aagcacgtcc ctccgagatg gcgcgtccat
      961 cgatcggaaa catccggttc atcttgctcc aatcacagcc ccagatcggt cgatcataat
     1021 cttcgttacc gcctgcaccg cggagcgacg gcagattctc gcttcaccga tagcgcatgc
     1081 ggcgatcgtt agggcatggg aacaagcgtc tgcctggctc gtcggtcgct atgttattct
     1141 acccgatcac gtccattttt tctgcgtatc gaacgcgagg ggcgcgctgt cgttggaacg
     1201 ctggattcga tattggaagt cgctggtgac gaaatcactg gaccaacgca gcgagacggt
     1261 ctggcaacgc catcactggg atcgccaact tcgtgccggc gaaagctatg ataacaagtg
     1321 ggaatacgtg cggagcaacc ccgtccgcca cggattaatg tcagacgctg acgcgtggcc
     1381 ctatcaaggc gaactgaacg aattacgatg gtgatgccga agcgacgtgc ttctgcacgt
     1441 cacgaactat tgaggacgca gcgaaaatgc gaggacgtgc ggaagcacgt ccctccgagg
     1501 cgatgaagat ttacttcatc gccggcgaag cgagcggcga caatcatggc gcagcgctca
     1561 tgcgctccct gcgcctgttg cggcctgacc ttcaattgtc aggccggggc ggtccgcaga
     1621 tgaaacagat cgccggctcg gaattcaaag attggatcga ccaagccggc gtcgtcggcc
     1681 tctgggaagt ggtcaagcat tacggttact ttcgacagga atttcggaaa acgctgcatg
     1741 agatcgcggc caccgcaccg aatgcggtcg tgctgatcga cttcccggga ttcaacctgc
     1801 ggctcgcgcg cgcgttgcgc gggacccgaa cgcttgcggg cttgggcggc aagattattt
     1861 actacatcag tccgcaggtt tgggcgtgga atcgccggcg cattccgcag atggcacgct
     1921 ggctcgacct gatgctttgc atttttcctt tcgaagccgg gctctacgaa aagtcgggcc
     1981 tgcgcacgat cttcgttggt catccgatga tcgagaatct gggggcgaaa cggaccgacg
     2041 cgctgcgcgc gccgtatctg gtcggtcttt ttcccgggag ccggacgcgc gaggtgcgca
     2101 agctgttgcc gatcctggtc gaggtcatgc gcaagctgca cgtgcagcga cccgaattgc
     2161 atttcgagat cgcggcggct tcggaggcaa tcgcgaccgc gatccggcgc gacttgcagc
     2221 cggttgtcaa ttcgttaggg caagtcgcgg ttgtcgtcgg ccagacggcg caaacgatgc
     2281 aacggagtgc ggtcggcgtc gtcgcctcgg gcacggcgac gctggaagcg gctttttttc
     2341 gactgccttt cgtgctcgtc tacaaggtgg cggcgctgac ctatcttgcc ggccggatgc
     2401 tgattcgggt aaaacatctc ggcatgccta acgttctcgc ggatcgcgaa attgtcgcgg
     2461 agttcatcca gggaaaagca caaccgcccg cgatcgccgc ggccgtgctg acgctgctcg
     2521 atgaccgcgc ccgccgcgaa cgcatgctcg ccgacttcga tgcggtcatc accaaacttg
     2581 gtaagggcgg cgcgaacgaa gcggcggccc gcgccatcct cgaagcggtc aacgaaaaat
     2641 gaacacgatc gaagaaagaa tttcccgcct ccacgccggc cgcctgatcg atctgcattt
     2701 cgatctgccg ctcattcttt ttctcagccg cccgcgccgg aacatcatcg cgccggattt
     2761 gctcccgcag tttgaggcgg gcgacatcgg cctgctcggt gtcgcgctct atgtcgcgga
     2821 ccaatatttg ggtgaaccag cgctgcgcgt ggtgctcgac caggtggcgc tgctcaaggc
     2881 ggagctggag acgacgccgc gcctcgtgct ttgccggacg tttgccgaaa ttgagcaggc
     2941 ccggcgggaa ggacggatcg cgttgctcct cacgatggaa ggcgccgagc cgctcggcga
     3001 cgatctaaat ctgctgcaca tttttcatga attggggctg cgcgccattt cactcaccca
     3061 tgcgagagcc aacgcggccg ccgccggcgg aatttttgcc gccaccggct caccggcaac
     3121 tggcctaacg agcttcggcc gcgaggtcgt acaggagtgc gagcgccttg ggattatgct
     3181 cgacctggcg cacatcaatc ccaccggctt cgaggaaatt tgcgcgctga cgagcaagcc
     3241 gttgatcgtt tctcacacca acgcgcgcca tttttacgac atcgaacgca acatcagtga
     3301 cgaccaaatc aagatgatcg gcgcgcgcgg tggcgtcatc ggaattaacg cgatcctggt
     3361 cagcgcgaag caggaggagg caacgctcga tgggtatgtc gaccacatcg agcatgtcgc
     3421 gaatctcatc gggatcgacg gcgtggcgat cggcttcgac ttttgcgagc tgctcctcgg
     3481 actgttgccg gccgcggagc gcgatgaact ggaagccaag ctaaccaagc cgcattatct
     3541 cccggacctg agccatcatg gccaggcccg caatctgacg cggaaattga tcgagcgcgg
     3601 tttcagcgat gaacagatcg agaaaattct ccgcgacaac tggctgcggg tgttgcggga
     3661 attcctctag acccaaggcc agcgttgctc ggacggaggg aagcgcttcc gcacgccccc
     3721 gcctcgaaat cgccatcccg gcggaagctt tctcggacat tccggacgtg cggaagcacg
     3781 tccctccgaa ggcgtttggc gaggtcggag agccgcatgt atagtcgcgc cattccagtg
     3841 ggcgcagaac cgatccagat ccagaatttc atcgacggcc gctttgtacc gcctctcggc
     3901 ggaaagtacc tcgacaacat cgagccc
//
LOCUS       DATMNS010000305         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3834525, whole genome shotgun sequence.
ACCESSION   DATMNS010000305 DATMNS010000000
VERSION     DATMNS010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3834525"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..622)
                     /locus_tag="VNW28_10785"
     CDS             complement(<1..622)
                     /locus_tag="VNW28_10785"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HXA10458.1"
                     /translation="MIAPDLRSGVEELGAMIAEASIIVPFTGAGISTECGIPDFRSPG
                     GLWTRNRPIPFDEFVANPDARDEAWRRRFAMESTFAAAKPGRGHRALASLYRAGKIPA
                     IVTQNIDNLHQRSGFAAEHVVELHGNTTYARCIGCGQAYDLGWVKQRFDEIGGAPDCT
                     LCDEPVKTATISFGQAMPEDAMRRATELAQHCDLFLAIGSSLVVWPA"
     gene            complement(1168..1377)
                     /locus_tag="VNW28_10790"
     CDS             complement(1168..1377)
                     /locus_tag="VNW28_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10459.1"
                     /translation="MRCLGEGKDTMPTYRAYLIDGDDRVASYKPIEADTDADALDAAR
                     QFLDGCDVEVWLLDRKIGRLEREKK"
     gene            1892..2341
                     /locus_tag="VNW28_10795"
     CDS             1892..2341
                     /locus_tag="VNW28_10795"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXA10460.1"
                     /translation="MRYLHTMLRVRNLDNALKFYRDALGLKEVRRVDNDKAKYTLVFL
                     CAPEDEGLLKAAKGRGAPLVELTYNWEEEKYGEDRYFGHLAYEVDDIYATCDRLKKMG
                     VTINRPPRDGQMAFVRSPDLHSIEILQKGEARPPAEPWASMPNTGHW"
BASE COUNT          536 a          745 c          731 g          454 t
ORIGIN      
        1 cggcgggcca gactaccagc gacgacccga tcgccaggaa caggtcgcaa tgctgggcga
       61 gctccgtggc gcggcgcatc gcgtcttccg gcattgcctg tccgaacgag atggtggcgg
      121 tcttgaccgg ctcgtcgcag agggtgcagt ccggcgcgcc gccgatttcg tcgaagcgtt
      181 gcttgaccca gccgagatcg taagcctgtc cgcagccgat gcagcgggcg taagtggtat
      241 tgccgtggag ctcgacgacg tgctcggctg cgaaacccga cctctgatgc aaattgtcga
      301 tgttctgggt gacgatggcc ggaatcttgc cggccctgta gagcgaggcc agcgcgcgat
      361 ggccgcgccc cggctttgcc gcggcgaatg tcgattccat cgcaaagcgc cgccgccatg
      421 cctcgtcccg ggcgtcagga ttggcgacaa actcgtcgaa tggaatcgga cggttgcggg
      481 tccataatcc gccgggcgag cggaaatcgg gaatgccgca ctcggtcgaa atgcccgcgc
      541 ccgtgaatgg aacaatgatg gaggcttcgg cgatcatggc gccaagctcc tccacgccgc
      601 tgcgaagatc cggtgcaatc atggaaatat ccgaacgtct ggccgcgccg gttatagcac
      661 ggccgcccgg aatgcacctg cgatgcgcga acgcggttgg caaccacaat cccgcaacgc
      721 cgtgccccaa ttcgcgcccc aatcgcggcc accaatgcgc gtgagacacg actcaaaaag
      781 ggactggtat gaccgaacag cagcaaagga ccgccgggga gcgggaggaa atcgcagcac
      841 gggttgcaaa cttcagggca acgcaggaaa aattcgcacg cgagcgcgag gaattttttg
      901 ccactacgct cgacaatgcg cgccactcct ctgaacgccc tccgactgtt tgggggcccg
      961 ttgattcgac aaagggctgt cgagaaaaaa cagccgttgc cggaaccaaa ccaccgaaac
     1021 caagttttcc tctcaagtgc agcaacctcg caagaggtga aagcatgaaa gaccccgtca
     1081 gcgttctcgg acgctggcgg ggttttcatt tttggctcgc gggctcctca cattgcggct
     1141 caactcacaa acggctgcaa cgattattta ctttttctcg cgctcgagcc gcccgatctt
     1201 ccgatcgagc agccagacct cgacatcaca gccatctaaa aactggcgag cggcatcgag
     1261 cgcgtcggcg tctgtgtcgg cctcgatggg tttgtacgaa gcaacccgat cgtctccatc
     1321 tattaggtaa gcccgatacg tcggcatggt gtccttccct tccccgagac aacgcatggg
     1381 ggaatggaac tgaaggaagg gggctatgca ggaaccgaat ggaaccggcg cgtgcggccg
     1441 gaaacggcgc ggaaaccatt gatcggcaaa acgattgtag aagtacgagc tttcttcgca
     1501 ttacctgatg agtgttcgca atgaatttat ctaccttgaa ggaacaagca gagcgttgcc
     1561 gtcgtctcgc aaaacacgcc gattccttta cccaaagaag gctgatggat cttgcctcgg
     1621 aatacgaggc ccaaatagca aagctcgagc ccgacctttc cgctgcatcc cgcgttgtgc
     1681 ggactggcca gcaatgaccg gtcggctcga cggggcgccg ttcgaccttc tctcaaagct
     1741 tcacatatcg agactagccg gcgaggttgc acaactgtgc gtgacgatcc gccgctagct
     1801 cgcgcaagct actgcacctg cccttcgttt gctgctattg agatttcgaa cccaaggcca
     1861 actcttcaat atctccaggg agacaggaaa catgcgttat ctccacacca tgctgcgtgt
     1921 ccgcaacctc gacaatgcgc tgaaattcta ccgggacgcg ctgggattga aggaagtccg
     1981 ccgcgtcgac aacgacaagg cgaaatacac gctggtgttc ctgtgcgccc ccgaagacga
     2041 gggcctgctg aaagccgcca agggccgcgg cgcgccgctg gtcgaactca cctacaattg
     2101 ggaggaggag aaatacggcg aggaccgcta tttcggccac ctcgcctacg aggtcgacga
     2161 catctacgcg acctgcgacc ggctgaagaa aatgggcgtc accatcaacc gcccgccgcg
     2221 cgatggccag atggcctttg tgcgctcgcc ggatctgcat tcgatcgaga ttttgcagaa
     2281 gggcgaggcg aggccgccgg ccgaaccctg ggcctcgatg ccgaacaccg gacactggta
     2341 gggcgctttc cgaagttccg aagccggaaa gccgcttgcc ggatcatcct tgtttccgtt
     2401 gctttggcgg gaacccaacc tcgcctgttg cgttcttgaa tcagtgaaga atgcattgga
     2461 ggagat
//
LOCUS       DATMNS010000306         9254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3196942, whole genome shotgun sequence.
ACCESSION   DATMNS010000306 DATMNS010000000
VERSION     DATMNS010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9254
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3196942"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..358
                     /locus_tag="VNW28_10800"
     CDS             125..358
                     /locus_tag="VNW28_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HXA10461.1"
                     /translation="MKPVQPDEKLSAIVGSKPLPRSELTKKLWDYIKKNGLQDKKKRT
                     QINADDAMRPVFNGKSQVSMFEMTKLVSGHLKG"
     gene            524..970
                     /locus_tag="VNW28_10805"
     CDS             524..970
                     /locus_tag="VNW28_10805"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXA10462.1"
                     /translation="MRTVRVAFLAGALCAFGLGTARAEVNWLSDFAKAQQEAKASHKL
                     LLLDFTGSDWCIWCLKLHREVFSQAEFEEYARKNLVLMTVDFPHAKPLSREVRKQNYE
                     LAQRYQVDGFPTIVVLNGDGRPVGLLGYMPGGPEAFINELKKLPKS"
     gene            complement(992..2884)
                     /locus_tag="VNW28_10810"
     CDS             complement(992..2884)
                     /locus_tag="VNW28_10810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA10463.1"
                     /translation="MRILLIEDHPDSRRNLRRLIEARGHEVVACASAEEAELEIAKES
                     FPFLILDWMLPGKSGVELCRDLRARRNGDERYILLVTARSDTDDLEQALAAGANDYLT
                     KPLDARRLGVRLSVAERHIRELEERNQARVALQESAQRMSDILEKTSDGFFALDPNWN
                     FTHLNAEAEVMLDAGRESLIGQNIWAKFPNLTGTIFELNYRKVMSEQKAIEFEALDSK
                     GKIWFEVHSYPSGGGISVFFRDITERRRADDERLTTSKLESLGTLAGGIAHDLNNILT
                     VISGNIGLAQLDAPAQSGNLLSFLAKAGQAAQHAARLSNQLLTFSKGGAPLKKVASVS
                     ELLKHAAEFSLHGSNLRAAIEIEDHLGKAEIDAGQVEQVINALMINAREAMPNGGSVN
                     VCAENVQIDHKSGLPLAPGRYVKIAIADHGPGVPTDLAAKIFDPYFTTKASSSGLGLS
                     ISYSIIKKHGGYLHLESNSPAGATFTLYLPAASGKVVNDPLQPNDRSFQRRPERILVM
                     DDESAIRELTSQLLATMGYEVTAVPDGSEAVRIYERALRKGEYFRAVILDATVRGGLG
                     GVETIERLRSIDPEVNAIICSGYSDEAALSQFLSYGFRWALPKPFTRRELADALEKAL
                     DSVPQN"
     gene            3114..3983
                     /locus_tag="VNW28_10815"
     CDS             3114..3983
                     /locus_tag="VNW28_10815"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXA10464.1"
                     /translation="MKLPPIIPSPFLAMALSLFLGLAESPAAPNSPVPESERAVGPAA
                     TAAATPALSYSAVHVNGPFIAMTFDDGPSAKLTPELLDILAQHHIHATFFVIGKNVVD
                     HPEILQRAAREGHEIGNHSWSHPAFAKMRDESVRAELQKTDDAIRAAIGARPVLMRPP
                     YGSITARQKHWINAEFGYRIILWDVDPLDWKRPGAAVVTSRIVRETRSGSIILSHDIH
                     PGTIQAMPAAFDQLQAKGFKFVTVSELIAMGKPMAPKQTGEEGAPPSPSPGGSPAATP
                     VPIATPGAGLTTP"
     gene            3993..5720
                     /locus_tag="VNW28_10820"
     CDS             3993..5720
                     /locus_tag="VNW28_10820"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HXA10465.1"
                     /translation="MVADLYLQFRSRTEAALSGLLKLAADMQRDPAMLDTLQNMHKDI
                     REPLLFVVVGEVKAGKSSLLNALFGQEFCKVDVLPATDRIYIFKHGSREQSVDVSGQV
                     TERYQPIAFLEDFNIVDTPGTNTMVAEHQTITENFIPRADLVLFVFSVVNPWGASAWE
                     LLRFVNQKWLKNIVFVLQQADLRDPREVEVIEQHLRETASQKLGFAPSIFAVSARKAF
                     LARTSGVDKERLWEESRFAPLEKHINLMVDASKHGLPKLITACQTATVILSDSAMRVR
                     AILETIVRDETQLGRLDGILETRKEQTLRQVGGFLRGIEQACRRCETEGQELLEHNLS
                     FWRTWRLVFGKAQWQEKFQVDLENKMRELIQPQVENALQLLETDLRSLWPQLQDTMEN
                     QFKGGARVQLSQTIPDFARQRRELLQSIELTLVERIAGRGMEAQLERMFRETANWLRV
                     PAGVAAAGGIVTLIAAMSSAAVADVTGIVAASAFVAGTLVAFGRRRKILSEYHRQMES
                     KREELIAAIEQQLQHAIDLFYGEIGSAFQPLRAFCAAERKRYDPLLNRVREIEKSLAE
                     LIADLGRSR"
     gene            complement(5840..6685)
                     /locus_tag="VNW28_10825"
     CDS             complement(5840..6685)
                     /locus_tag="VNW28_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10466.1"
                     /translation="MLAKQTLMLMTLAACGPATATMAWAQSPYESSADFAKYAMKLRA
                     QALLKVEPQVFVPTTSRFSTSRYPWKTDIVTTIFWIGEMPTANNPVPNHKSSWDADWA
                     RNYGGYDNPDPSRRHDYIPISFVPRQNPFYCALPYNDVTHGQFKPEAPLVVPWFRQAF
                     VQQGHSVCKDRWIAIRRGNRVCYAQWEDCGPFRTDHYQYVFRNDRPRMNLNHGAGLDV
                     SPAVRDYLGLGPTSLTDWQFVEVRDVPPGPWRNYGDNNNFVIARRQTEQRLAEQYSTK
                     TKAKK"
     gene            complement(6770..8368)
                     /gene="nadB"
                     /locus_tag="VNW28_10830"
     CDS             complement(6770..8368)
                     /gene="nadB"
                     /locus_tag="VNW28_10830"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HXA10467.1"
                     /translation="MREFDFVVIGSGIAGLTFAIKAARHGRVAIITKRKGVDSNTAWA
                     QGGVACVTSGEDSFELHIRDTLEAGAGLCDPAAVETVVKEGPERVRELMELGLHFDER
                     DVAGHRELDLAREGGHSKRRVLHVHDLTGFEIETTLLRAAQQSPNIELLENHMAVDLI
                     TAAKLGFATEDHCLGVYVLDEISGKVETIRTDRLLLATGGCGKVYLYTTNPAIATGDG
                     VAMAWRAGAVIANMEFIQFHPTCLFHPQATSFLISEAVRGEGGILRNGKGEDFVARVH
                     PRGSLAPRDVVARAIDAEMKRSGAQCVYLDITHQTPEFLQERFPHIHETCLRHGIDMA
                     KEPIPVVPAAHYQCGGIKTNMDGATSLPGLSAIGEVACTGLHGANRLASNSLLEGLVI
                     AHRACEALVRSRPPAHPAGSVSLPEWQSGDVQDVDELVVIYHNWDEIRRLMWDYVGIV
                     RTDKRLQRASARLRNLRREIQEFYWNFRISVDLLELRNLATVAALIVDSALSRKESRG
                     LHYTMNYPVMRDDVFRRDTLLSRG"
     gene            complement(8365..>9254)
                     /gene="panC"
                     /locus_tag="VNW28_10835"
     CDS             complement(8365..>9254)
                     /gene="panC"
                     /locus_tag="VNW28_10835"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HXA10468.1"
                     /translation="SSLSFCPAGDLVGTSPAKMKIFSTVAKLRAHPRHQNRRRILVPT
                     MGALHAGHISLIRLARQRAGTAGEVAVSIFVNPLQFEPGADFSRYPRPESADAETCRA
                     EGVDVLFRPAPNEMYADDRSTHVEETILSEKLCGASRPGHFRGVCTVVTKLFHLLEPH
                     AAVFGEKDFQQLAIVRRLVRDLNFPVEIIAGPTIREPDGLAFSSRNQYLNAEERAQAP
                     ILRAALLEAAHSRENSSASILTTVRTKIESAPLARIDYAEIVSADDLQPRLTIKPRSL
                     LAVAVFFGRTRLIDNILLG"
BASE COUNT         1833 a         2800 c         2772 g         1849 t
ORIGIN      
        1 taatattttt tttcgccgga gtaaaattag tattgctctc gtgcagccca acgattaatc
       61 agcctctatg cccaaatcca cagccaaaaa aacgccctcg aaacgcaaac ccaacgcagc
      121 ttttatgaag cccgttcagc cggatgaaaa actctccgcg atcgttggct ccaagccgct
      181 tccacgctcg gaattgacca agaagctctg ggattacatc aagaagaacg gcctgcagga
      241 caaaaagaaa aggacgcaaa tcaacgccga cgacgcgatg cgtccggtct tcaacggcaa
      301 gagccaggtg agcatgtttg agatgaccaa gctcgtctcc ggtcacctca aaggctaatc
      361 gcctaacgaa tccgttcggc ttaacggctg tatcggaggg acaatatttg gtcgcccgcg
      421 agcagttgtt ctgctcgcgg gtgctcccct gtcccccgga gaaactcgcc tgaacgcagc
      481 gcttcgcctt gcggagagtc ttctccacgg cgactggtta ccggtgagaa ccgttcgagt
      541 cgctttcctc gccggggcgc tttgcgcctt cgggctcggc acggcccggg cggaagtgaa
      601 ctggttgtcc gatttcgcca aggctcagca ggaggcgaaa gccagccaca agctgctcct
      661 gctcgacttc accggttcgg actggtgcat ctggtgtttg aagctgcacc gggaggtttt
      721 ctcgcaggcg gaatttgagg aatatgcgcg gaaaaatctc gtcctgatga cggtggattt
      781 cccgcacgcc aagccgctga gccgtgaggt gcggaagcag aattatgaac tcgcgcagcg
      841 ttatcaggtg gacgggttcc cgacgatcgt ggtcttgaac ggcgatggtc ggccggtcgg
      901 cttgctcggt tacatgccgg gaggaccgga ggctttcatc aacgaactga agaagctgcc
      961 gaagagttag ttggcctaac gaattgcccg atcagttctg cggcacgctg tccagcgctt
     1021 tttcgagggc atccgccagc tcgcgccggg taaatggttt cggcaacgcc cagcggaatc
     1081 cgtaggaaag aaattgcgag agagccgctt cgtcggaata accgctgcag atgatcgcat
     1141 tgacctccgg gtcaatgctc cgcaggcgct caatcgtttc cacgccgccc aggccgcccc
     1201 ggaccgtggc gtcgaggatg accgcccgaa aatactcacc tttgcgcagg gcgcgctcat
     1261 aaatccggac cgcttccgag ccgtccggca ccgcggttac ttcgtaaccc atcgtcgcga
     1321 gcagttgcga ggtcagttcc cggatggccg attcgtcatc catgaccagg attcgctcgg
     1381 gacggcgttg gaatgaacgg tcgttaggct ggagcggatc gtttaccacc ttgccgctcg
     1441 ccgccgggag gtaaagcgta aaagtagcgc cggccggcga gttgctttcg aggtggaggt
     1501 agccgccatg ttttttgatg atcgaatagc tgatcgacaa accgagaccg ctcgaggaag
     1561 ccttggtcgt gaaataagga tcgaaaattt ttgcggccag atcggtcggc accccgggac
     1621 cgtggtccgc gatggcgatt ttgacgtagc gaccgggcgc caggggcaat ccacttttat
     1681 ggtcgatctg cacattctca gcgcagacgt tgaccgagcc gccattgggc atggcttcac
     1741 gcgcattgat catcaaggcg ttgatgactt gctccacctg accagcgtcg atttccgcct
     1801 tgccgagatg atcctcaatc tcgatcgcgg cgcgcagatt ggatccgtgt aaggaaaatt
     1861 ccgccgcgtg cttcaataat tccgagaccg aggcgacttt tttcagaggt gcgccgcctt
     1921 tggaaaaggt gagcaactgg ttggaaaggc gagccgcatg ttgggccgct tgccccgcct
     1981 tggcgaggaa ggagaggagg ttgccactct gggccggggc gtcgagttgg gcgagcccga
     2041 tgttgcccga gatgacggtg agaatgttgt tcaagtcgtg cgcgattccg ccggcgagcg
     2101 tgcctaacga ctcgagcttg ctcgttgtca gccgctcgtc gtccgcccgc ctgcgttcgg
     2161 tgatatcgcg gaaaaagacg gagatcccgc cgccgctcgg ataggaatgc acctcgaacc
     2221 agatcttccc tttactgtcg agcgcttcaa attcgatcgc cttctgctcg ctcattacct
     2281 tgcgataatt cagctcaaaa atcgtgccgg tgaggttcgg aaacttggcc cagatgttct
     2341 gcccgatgag cgactcgcgt ccggcatcga gcatgacctc cgcttccgcg ttcaggtggg
     2401 taaagttcca attcggatcg agcgcaaaga acccgtcgct cgttttctcc agaatgtcac
     2461 tcatgcgctg ggctgattcc tggagtgcga cccgggcttg attgcgttcc tcaagctcgc
     2521 ggatgtggcg ctccgcgacg gagagccgca cgcccaaacg gcgggcatcg agtggtttcg
     2581 tgagatagtc attcgcgccc gcggcgagcg cctgctcgag atcgtcggtg tcggaacgcg
     2641 cggtcacgag caggatgtac ctctcgtcgc cattccgccg cgcccgcaag tcgcggcaaa
     2701 gctcgacgcc gcttttgccg ggcaacatcc aatccaaaat caaaaacggg aaactttctt
     2761 tcgcgatttc cagttccgct tcctcggcgc tggcacaggc gactacttcg tgcccgcggg
     2821 cttcgatcag ccggcgcaga ttgcgccggc tgtcgggatg gtcctcaatt agaagaattc
     2881 tcatggccag tttaaaattt ttcttgctcc ccagacctct cgtgcggccc tgccgtgttg
     2941 gatcgggaga gcggctcgcc tgcaacctcg ttatgagcag gatgactgcc aaggccgcgg
     3001 caagtattgt taaagatacc cctcctcggc ggcggttaca tccgttacgc ccaggaggcc
     3061 cgttcccttg cgttgatttg ctcgtctggt agatacagcg caaatcttta tttgtgaaat
     3121 tgccgccgat catcccgagc ccgtttctgg ctatggccct ctcactgttc ctcggcctcg
     3181 ccgaatcgcc cgctgcgcca aattcgcccg tgcctgagag cgagagagcg gtcggcccgg
     3241 ctgcgacggc ggcggcaaca ccggcattaa gttacagcgc cgtgcatgta aatgggccct
     3301 tcattgcgat gacgtttgac gacggcccga gcgcaaagct cacgcctgaa ttgctcgaca
     3361 tccttgctca acatcacatt cacgcgactt tcttcgtgat cggcaagaat gtggttgacc
     3421 accccgaaat cctgcagcgc gccgcgcgcg aggggcatga gattggcaat cacagctggt
     3481 cacaccccgc ttttgccaag atgcgggatg aaagcgtgcg ggctgagttg caaaaaacgg
     3541 acgatgcaat ccgggccgcg atcggcgccc ggcccgtcct gatgcgaccg ccctacggct
     3601 cgattaccgc gcggcagaag cactggatca atgcggagtt cggttatcgc atcattcttt
     3661 gggacgttga tccgctcgac tggaaacgac cgggtgcggc ggtggtcacg agtcgcatcg
     3721 ttagggaaac acgttccggg tcgatcattc tttcccacga tattcatccg ggcacgatcc
     3781 aggcgatgcc ggccgcgttc gatcaactcc aggcgaaagg gttcaaattc gttactgtgt
     3841 ccgagttaat tgcgatgggg aaaccgatgg cgccgaaaca aaccggtgaa gagggcgcgc
     3901 cgcccagtcc ttccccaggc ggcagtccgg cggcgacccc ggttccgatt gccacccccg
     3961 gcgctggcct aacgactccg tagagcgccg gcatggttgc cgacctttat ctccagtttc
     4021 gcagtcgcac ggaggcggcc ctctccggat tgctcaagct ggcggccgac atgcagcgcg
     4081 atccggccat gctcgatacc ctgcagaaca tgcacaaaga catccgcgag cctctgcttt
     4141 tcgtcgtcgt cggggaagtc aaagcgggca aatcatccct gctcaacgcg ctctttggcc
     4201 aggaattctg caaggtcgac gtgctacccg cgaccgaccg catctacatc ttcaagcacg
     4261 gatcgcgcga gcaaagcgtc gatgtctccg gacaggtgac ggagcgctat cagccgatcg
     4321 cgtttctcga ggacttcaac atcgtcgata cgccgggcac gaacacgatg gtggccgagc
     4381 accagacgat cacggaaaat ttcatccccc gtgccgacct cgtcctcttc gttttttcgg
     4441 tggtcaatcc atggggggcc tccgcctggg agttgcttcg gttcgtcaac caaaaatggc
     4501 tcaagaacat cgtcttcgtc ctgcagcagg ccgatctgcg cgacccgcgc gaggtcgagg
     4561 tgatcgagca gcatctgcgg gaaaccgcca gccaaaagct cggcttcgcg ccttcaatct
     4621 tcgcggtttc cgcgcggaaa gcgttcctcg cccgcacttc cggggtcgat aaggaacggc
     4681 tttgggagga gagccgcttc gctcccctgg aaaagcacat caacctgatg gtcgatgcat
     4741 ccaaacacgg cctgcccaag ttgatcaccg cctgccagac tgcgaccgtc atcctcagcg
     4801 acagcgccat gagggtgcgt gccattctcg agacgatcgt gcgcgacgaa acccagctcg
     4861 gccgcctgga cggaattctc gagacgcgga aagagcaaac tttgcgacaa gtgggcggct
     4921 tccttcgcgg gatcgagcag gcgtgccgtc gctgtgaaac cgaagggcag gaattgctcg
     4981 agcacaatct ctccttctgg cgcacctggc ggctcgtttt cggcaaagcg cagtggcagg
     5041 aaaaatttca ggtcgacctc gaaaacaaga tgcgggagct gattcaaccg caggtcgaga
     5101 acgcgctgca actcctcgag accgatctgc gcagcctctg gccacagttg caggacacga
     5161 tggagaatca gttcaaaggc ggcgcgcgcg tgcagctcag ccagacgatc ccggattttg
     5221 cgcggcagcg gcgcgagctc ttgcaatcga tcgagcttac gctcgtggaa cggatcgccg
     5281 ggcgcggcat ggaagcgcag ctcgagcgca tgttccgcga aacggccaac tggttgcgcg
     5341 tgcccgccgg ggtcgccgcc gccggcggca tcgttaccct gattgcggcg atgagcagcg
     5401 ccgcggtcgc cgatgtaacg ggaattgtgg ccgcgtctgc cttcgtcgcc gggactctcg
     5461 tcgccttcgg acggcgccgc aaaattctct ctgaatatca ccggcaaatg gaatcgaaac
     5521 gcgaggagtt gatcgctgcg atcgagcaac aattgcagca cgcgatcgat ctcttctacg
     5581 gggagatcgg ttcggccttt cagccgctcc gcgctttctg cgcagctgaa cgaaaacgct
     5641 acgacccgct cctgaatcgc gtgcgggaaa tcgagaagag cctcgcggaa ttaatcgctg
     5701 acctggggcg aagcagataa aacgtggtgc gaccgaacct ccgttgtcgc cggggtcgct
     5761 gaccccggtc tttggttcga gagcgcgtgg cgccggctgc ggtcaccgat cccggctacg
     5821 agaagaacga gcgtcggccc tacttcttgg cttttgtttt ggtcgagtat tgctcggcca
     5881 ggcgctgctc ggtctggcgg cgggcgatga cgaagttgtt gttgtcgccg tagttccgcc
     5941 acggaccggg cggcacgtcg cgcacctcca caaattgcca atcggttaac gacgttggcc
     6001 cgaggccgag ataatcgcgc accgccggcg acacatccag cccggcgcca tggttcagat
     6061 tcatcctggg ccggtcgttc cggaaaacat actggtaatg gtcggtccgg aagggcccgc
     6121 aatcctccca ttgtgcgtag cagacccggt tgccgcggcg gatcgcaatc caccgatctt
     6181 tgcagaccga atggccctgt tgcacgaagg cctgccggaa ccacggcacg acgagcggtg
     6241 cttccggttt gaactgcccg tgagtgacat cgttataggg cagcgcgcag taaaacgggt
     6301 tctgccgcgg tacgaaactg atcggaatgt aatcgtgccg gcgcgacggg tcagggttgt
     6361 cgtagccgcc ataattgcga gcccaatccg cgtcccatga gcttttgtga ttcggcaccg
     6421 ggttattcgc ggtcggcatt tcgccgatcc agaaaatggt tgtaacgatg tcggtcttcc
     6481 acgggtagcg ggaggtcgag aagcgcgagg tcgtcggcac gaaaacctgc ggctcaactt
     6541 tgaggagcgc ttgggcgcgc aatttcatcg cgtatttagc gaaatcagcg ctgctttcgt
     6601 aaggcgactg cgcccacgcc attgtcgcgg tcgccgggcc acaggcggcc agcgtcatca
     6661 gcatgagtgt ttgtttcgcc agcatcgcca cgtttcttct agtcagattg gccgcacagg
     6721 caaggcccgt ttcataatcc cctcttagca gaatccattc caaaaacgct caaccgcggc
     6781 tcagcaaggt gtcccgccgg aaaacgtcgt ccctcattac gggataattc atcgtataat
     6841 gcaagcctcg actttccttt cggctgagcg cggaatcgac gatcagggcg gcgacggtgg
     6901 cgagattgcg caattcgagg aggtcgaccg agatacggaa gttccagtaa aactcctgga
     6961 tctcgcgccg tagattgcgc agacgggcgc ttgcccgctg cagccgtttg tccgtgcgca
     7021 cgatgccgac gtagtcccac atcagacgcc ggatctcgtc ccagttgtgg tagatcacga
     7081 ccagctcgtc cacatcctgg acgtcgcccg attgccattc cggcagtgaa accgatccgg
     7141 ccgggtgggc cggcgggcgg ctgcggacca gcgcctcaca agcgcgatgt gcgataacca
     7201 gcccttcgag taacgaattg ctcgccaggc ggttggcgcc gtgtaatccg gtgcacgcca
     7261 cttcgccgat cgccgacaat ccaggcaaac tggtcgcgcc gtccatattc gttttgatcc
     7321 cgccacattg gtaatgcgcg gcaggcacga ccgggatcgg ctccttcgcc atgtcgatgc
     7381 cgtgacgcaa gcaggtctca tgaatatgcg ggaagcgctc ctgcaaaaac tcgggtgtct
     7441 gatgggtaat atcaagatag acgcactgcg cgcctgagcg tttcatctcg gcgtcgatcg
     7501 cccgggcgac cacgtcgcgc ggcgccagcg acccccgtgg atgcacccga gccacgaaat
     7561 cctcgccttt gccgttgcgc aagattccgc cttctccgcg caccgcctcg gaaatcaaaa
     7621 acgacgttgc ctgagggtgg aaaaggcagg tcggatgaaa ctggatgaat tccatgttgg
     7681 cgatgaccgc accagcccgc caggccatcg ccactccgtc cccggtcgcg attgctgggt
     7741 tggttgtgta gagatacact tttccgcagc ctcccgtcgc cagcagcagg cgatcggttc
     7801 ggatggtctc caccttgccg gaaatttcgt cgagcacata gacgccgaga cagtgatctt
     7861 cggtcgcaaa gccgagtttg gcggcggtga tcaagtcgac cgccatgtgg ttttcgagca
     7921 gctcgatgtt aggcgattgc tgcgccgcgc ggagcagggt cgtctcaatc tcgaagccgg
     7981 tgagatcgtg gacgtggagg acgcgtcgtt ttgaatgccc gccctcccgg gcgaggtcga
     8041 gttcgcgatg acccgcgacg tctcgctcat cgaaatgcag cccgagttcc atcagctcgc
     8101 gaacccgctc cgggccttcc ttgaccactg tctcgaccgc cgccggatcg cacaaacccg
     8161 cgcccgcctc gagcgtgtcg cgaatgtgca actcgaagga atcctccccg ctcgtgacac
     8221 aggcgacccc tccttgcgcc caggcggtgt tcgagtccac gcctttgcgt ttcgtgatga
     8281 tcgccacgcg gccgtgcctg gccgccttga tcgcaaacgt gaggccggca attccgctgc
     8341 cgatgacgac aaaatcgaat tccctcatcc gagcagaatg ttgtcgatca agcgcgtcct
     8401 gccaaaaaac accgcgacgg cgaggagcga gcgcggtttg atcgtgagcc gcggctgcag
     8461 atcatcggcg cttacgatct ccgcataatc gatccgggcg aggggtgccg attcgatttt
     8521 cgtccgcaca gtcgtgagaa tgctggcaga agaattctcc cgggaatgcg ccgcctccag
     8581 caaagccgcc cggagaatcg gcgcctgcgc gcgttcctcc gcattgagat actgattgcg
     8641 cgagctgaag gcgaggccgt ccggttcccg aatcgttggg ccagcgatga tctcgaccgg
     8701 aaaattgaga tcgcgcacga ggcggcgaac gatggcgagc tgctgaaaat ccttttctcc
     8761 aaaaaccgcg gcgtgcggct cgaggagatg gaacaatttt gtcaccacgg tacagacgcc
     8821 gcgaaagtgg ccgggccggg aggcgccgca gagcttctcc gacaggatcg tttcctcgac
     8881 atgggttgaa cgatcatcgg catacatctc gttaggcgca gggcgaaaga gaacgtccac
     8941 gccctcggcc cgacaggtct ctgcgtccgc cgattccggc cgcgggtaac gcgaaaaatc
     9001 cgcgcccggt tcgaattgca gcgggttcac gaagatactg accgccactt cgccagcggt
     9061 gccggcacgt tgccgtgcca gtcgaatgag cgagatgtgt ccggcgtgca aagcgcccat
     9121 cgtcggaacc aaaatccggc gtctgttttg gtggcggggg tgcgcgcgca gttttgccac
     9181 ggtggaaaag attttcatct tcgccgggga cgttccaacc aaatcgcctg ccgggcaaaa
     9241 cgaaagcgac gatg
//
LOCUS       DATMNS010000307         5768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2134547, whole genome shotgun sequence.
ACCESSION   DATMNS010000307 DATMNS010000000
VERSION     DATMNS010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2134547"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VNW28_10840"
     CDS             complement(<1..263)
                     /locus_tag="VNW28_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HXA10469.1"
                     /translation="MLNYIWLALILLAVTLGGATGHLKEVTDGAFDGAKTAVTIALGL
                     IGIMALWLGVMRLAERAGLIQKLARGLCPVMRRLFPGVPNDHPA"
     gene            complement(369..2027)
                     /gene="recN"
                     /locus_tag="VNW28_10845"
     CDS             complement(369..2027)
                     /gene="recN"
                     /locus_tag="VNW28_10845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HXA10470.1"
                     /translation="MPLLSLLRIKNLALVEDLEWELTPGFTAITGETGAGKSIIIGAL
                     QLLLGERADKALIRTGAEACTVEAVFVGEDFGALNTRLEESGIEPCAGELIVKRSFSL
                     GGSNRQFVNGSPTTLALLKTIGDELVDLHGPHDHQSLLSPERQLALLDAFAHAELARQ
                     EYQQCFRRRQALVAEEQSLRTAESAREQELDLLRHQVTEITSAKLQPNEEEGMVARYK
                     LASNSRRLIELATAVSRQLSESDNAVLSRLAETQRLLRELEKIDHGLTESSQAHAAAI
                     IELTEIARTLDRYAERLDLDPGQLAQLEQRVTLLESLKRKYGGSIPEVIAFGERAAER
                     MRKIEGRDAELERLAEEIREAWAALARAAEALRKARAKAAPRLAENVRRNLADLGFRQ
                     SGFEARLTPYDEPRLNGTDALELIFSPNPGEPLKPLRLIASSGEISRLMLAIKSALAM
                     QDAIPLLVFDEIDANVGGEIAHAVGAKMRALAEKHQVLCITHLPQVAGAAQTHFVVTK
                     AVTHGRTFSELRAVKGKARQEEIARMLGGKSESALKHAASLLKG"
     gene            2158..2889
                     /locus_tag="VNW28_10850"
     CDS             2158..2889
                     /locus_tag="VNW28_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXA10471.1"
                     /translation="MIRCDCAVCRSSDPRDNRTRASIYLQTPECAFVVDTGTDFRAQC
                     LRENIRRVDAVVMTHAHTDHVMGFDDLRRFAAPRGGRMPVYASAETMADLERVYEFAF
                     KTAHPSPGYLKPEPHIISGPFSLGETTITPLPVPHGSSETYGYLFSRHGRRLLAYLSD
                     CSAVPDEIARQIAGVQLLVIDALRHKPHPTHLSIAQALEVAQRVQPARTLFTHICHEL
                     PQAAEAELPPPTGIAYDGLKIDFPE"
     gene            2886..4469
                     /locus_tag="VNW28_10855"
     CDS             2886..4469
                     /locus_tag="VNW28_10855"
                     /inference="COORDINATES: protein motif:HMM:TIGR03790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03790 family protein"
                     /protein_id="HXA10472.1"
                     /translation="MRSLLCAIIVCSLGMRVAAAESGANLASATIVIYNPAAPESAGL
                     ARFYAQARQIPSDHLIGLECSTNEEISRQQYDETIAEPLRKIFAQRKWWTLRTNADQR
                     IAVTANSIRFVALVRGMPLKIRSTVGYPGDQKENNPIGEQNQASVDSELATLGFFSRR
                     ISGAAINPYFQNFRPVTELEGSPMMLVCRLDAPTAEIVRRMITDAVETEKSGLWGRAY
                     VDGADHSAGGLAEGDQWLTAIVKDLRRVGIPVVYDTEPTLFPSGFPMSDCALYYGWYA
                     GDVTGPFNDPGFVFPRGAVAVHIHSFSASTLRSVSAGWVGPLLSKGAAVSLGNVYEPY
                     LQLTAHLDIFNDRLLHGFTFAESAYMSIRVLSWMGVAVGDPLYRPYASWLQLDAKRDN
                     RKKSDWRMYHDFAVKNSQREPADYRATARAAASRADNGPLIEDLGLMEKEDGHFAAAV
                     SYLQQARSLYTKPDDILRTVLEQADALIKSGDRKAALALVRSVAQLVPDASATALLRK
                     IEQQLNPPSLAPTPSPSPL"
     gene            complement(4558..4923)
                     /locus_tag="VNW28_10860"
     CDS             complement(4558..4923)
                     /locus_tag="VNW28_10860"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXA10473.1"
                     /translation="MDATGYGDFRARREATIWQRMNHVLRVLLFIAVWLLIVSFFLPP
                     YKKLNQNRAEIDQLQAQLTDQQTLLARQTKQVTLLKSDPTYLETIARDSLELMKEGET
                     IFRLESRRTEPKRPNPPQP"
     gene            complement(4923..>5768)
                     /gene="eno"
                     /locus_tag="VNW28_10865"
     CDS             complement(4923..>5768)
                     /gene="eno"
                     /locus_tag="VNW28_10865"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXA10474.1"
                     /translation="PMMNILNGGAHSDAPIDFQEFMIMPKGAPTFPEALRYGAEVFHA
                     LKKVLHDRGLSTAIGDEGGFAPNLKSVEDALESIAAAVEQAGYSLGEQIFIALDPASS
                     EFFDQEQNAYVFKKSDGSKRTAAELVDYYASLCARFPIISIEDGCAENDWDGWKKLTD
                     RLGAEVQLVGDDLFVTNVQFLRKGIDLGVANSILVKVNQIGTLTETFATIDLAKQNDY
                     TAVISHRSGETEDTTIADIAVATNAGQIKTGSLCRTDRVAKYNQLLRIAEELGDKAVF
                     GGKMR"
BASE COUNT         1065 a         1851 c         1731 g         1121 t
ORIGIN      
        1 gccgggtgat cgttaggcac gcccggaaac aaccggcgca tcactgggca cagaccgcgc
       61 gccagttttt gaatgagccc ggcgcgttcc gccagtcgca tcacgcccaa ccagagcgcc
      121 ataattccga tcaaaccgag cgcgatcgtc accgccgtct tcgccccatc gaacgcgccg
      181 tcggtcacct ccttgagatg acccgtcgcg ccgcccagcg tgaccgcgag gagaatgagg
      241 gcgagccaga tgtagttgag cacgcctaat ctctaacaga ggagaaggcc ggggaaaacg
      301 ttcaacgtcc aaagaaggaa gaatggcccg caggcttgct cttcattgaa gcgttggacg
      361 ttgaacgttt atcccttcaa caaagaagcc gcatgtttga gcgccgattc gctcttgccg
      421 cccagcatcc gcgcgatttc ctcctgacgc gcctttcctt tcaccgcgcg caattccgag
      481 aaagtccggc cgtgggtcac cgctttcgtc accacgaaat gggtttgcgc cgcgcccgcc
      541 acctgcggca gatgcgtgat acaaagcacc tggtgtttct ccgccagcgc tcgcattttc
      601 gccccgaccg cgtgcgcgat ctcgccgccg acgttggcat cgatctcatc aaagacgagc
      661 aacggaatcg catcctgcat cgcgagcgcg gacttgattg ccagcatcaa acgcgagatc
      721 tcgccgctcg aagcaatcaa gcgcagcggc ttgagcggct cgcccggatt cggcgagaaa
      781 atcagctcga gcgcatcggt gccgttgagc cgcggctcgt cgtaaggcgt gagccgggct
      841 tcgaatcccg actgccggaa tccgaggtcc gccagattgc gccgaacgtt ttcggccagc
      901 ctcggcgcgg ccttcgcccg ggccttgcgc agagcctcgg ccgcccgcgc gagcgccgcc
      961 cacgcctccc tgatctcttc cgccaatcgc tccagctccg cgtcgcgccc ctcgatcttg
     1021 cgcatccgct ccgccgcccg ctcaccgaaa gcaatcactt ccggaatcga gccgccatat
     1081 tttcgcttca gcgattcgag cagggtgacc cgctgttcga gttgcgcgag ctgccccggg
     1141 tcgaggtcga ggcgttcggc ataacgatcg agggtgcgcg cgatttcggt caactcgatg
     1201 atcgccgcgg catgagcctg ggaactttcg gtcaggccgt gatcgatttt ttccagttcg
     1261 cgcaaaaggc gctgggtttc cgcgagccga ctcagcacgg cgttatctga ctccgagagt
     1321 tgtcgtgaaa ccgcggtcgc caactcgatc aggcgacggc tattgctcgc cagcttgtag
     1381 cgcgcaacca ttccctcctc ttcgttaggc tgaagttttg ccgaggtaat ctcggtcacc
     1441 tgatggcgga gcaggtcaag ctcctgttcg cgcgccgatt ccgccgtgcg gagcgattgt
     1501 tcctcggcca cgagcgcctg ccggcggcga aaacactgct ggtattcctg ccgggcgagc
     1561 tcggcatgcg cgaaagcgtc gagcaaggcc agctgacgct ccggcgagag aagcgactgg
     1621 tgatcgtgcg ggccgtgtaa atccaccagc tcgtcaccaa ttgttttcag gagggcgagg
     1681 gtcgttggcg agccgttgac aaactgccgg ttgcttccgc ctagcgagaa gctgcgtttc
     1741 acgatcaatt cgcccgcgca cggttcgatc ccggattcct cgagccgggt gttgagggcg
     1801 ccgaaatctt cgccgacgaa aaccgcctcc accgtgcacg cttcggcgcc ggtgcggatg
     1861 agcgctttgt ccgcccgttc gccgaggagc aactgcagcg cgccgatgat gatcgatttg
     1921 ccggcgccgg tttcgccggt gatcgcagtg aagcccggcg tcaattccca ctccagatcc
     1981 tcgaccagcg caaggttctt gatccggagc aaactcagca aaggcattcg gagagttgag
     2041 agttgagggt tgagagttga gagatagcct ctgaacgcct ccgactctca actcgcaact
     2101 ctcaactctc aactgactct cacctttctc gggaccggca cttcgcaagg cgtcccgatg
     2161 attcgttgcg actgcgccgt ctgccgctcg agcgatccgc gcgacaaccg cacccgcgcc
     2221 tcgatttatt tgcaaacacc ggaatgcgcc ttcgtggtcg acaccgggac ggatttccgc
     2281 gcccaatgtc tgcgcgaaaa catccggcgg gtcgatgccg tcgtcatgac gcacgcccac
     2341 accgaccacg tgatgggctt cgatgacctg cggcgtttcg ccgcgccgcg cggcggccgc
     2401 atgccggtct acgcttcggc cgaaacgatg gccgacctcg agcgcgttta cgagtttgct
     2461 ttcaagacgg cgcacccctc gcccggctac ctgaaaccgg agccgcatat tattagcggt
     2521 ccgttttcgt taggcgaaac gaccatcacg ccgctcccgg taccacacgg cagctccgaa
     2581 acctacggct acctcttctc ccgccacggg cgcaggttgc tcgcctacct gagcgattgt
     2641 agcgcggtgc cggacgagat cgcgcgccag atcgcaggcg tacaattgct cgtgatcgat
     2701 gctctgcgcc acaaacccca tccgacccat ctcagcatcg cccaggctct ggaggttgcg
     2761 cagcgcgtgc aaccagcccg cacccttttc actcacatct gccacgagtt gccgcaggcc
     2821 gcggaagcgg aactgccgcc cccgaccggc atcgcctacg atggactgaa gatcgacttc
     2881 ccggaatgag gagtctgctc tgcgcaatca ttgtctgctc gttaggcatg cgcgtcgcgg
     2941 ccgccgagag cggagcgaat ctcgcgtcgg ccacgattgt gatctacaac ccggcggcgc
     3001 cggagtcggc cggcctggcc cgcttttacg cgcaggcgcg acaaattccc agcgatcatc
     3061 taatcggcct cgaatgttcg accaacgagg aaatcagccg ccagcaatac gacgagacga
     3121 tcgccgaacc actccggaag attttcgcgc aacgaaaatg gtggacgctg cgcaccaacg
     3181 ccgaccagcg catcgccgtg accgcgaaca gcatcaggtt tgtcgcgctg gtgcgcggca
     3241 tgccgcttaa gattcgctcg accgtcggtt atcccggcga ccagaaagaa aacaacccga
     3301 tcggcgaaca gaaccaggcc tcggtcgatt cggaactcgc gacccttggc ttcttttcgc
     3361 gccggatctc gggcgcggcc atcaatccct actttcagaa ttttcgcccg gtcacggaac
     3421 tcgagggatc acccatgatg ctggtctgcc ggctcgatgc gccgacggcc gagatcgtta
     3481 ggcgaatgat caccgatgca gtcgagaccg agaagtccgg gctttgggga cgcgcctacg
     3541 tcgatggcgc ggaccacagc gccggggggt tggccgaagg cgatcaatgg ctgaccgcga
     3601 tcgtcaagga tctgcgccgc gtcggtatcc cggtggtcta cgacactgag cccacgcttt
     3661 tcccgtcggg cttcccgatg agcgactgtg cgctttatta cggctggtac gcgggcgacg
     3721 tcacgggacc gttcaacgat ccgggtttcg tcttcccccg cggcgcggtc gcggtgcaca
     3781 tccattcttt tagtgcgagc acgctgcgct cggtgagcgc cggctgggtc gggccgcttc
     3841 tgagcaaggg cgccgccgtg tcgttaggca acgtctacga gccgtatctg caattgaccg
     3901 cgcacctcga catttttaac gaccgcctcc tgcacggttt tacctttgcc gaaagcgcct
     3961 acatgtcgat ccgggtgctt tcgtggatgg gcgtggcggt gggggacccg ctctaccggc
     4021 cttatgcctc ctggttgcaa ctggacgcga aacgcgacaa ccggaagaag agcgactggc
     4081 ggatgtatca cgacttcgcg gtcaagaata gccagcgcga gccggcggat taccgggcca
     4141 cggcgcgcgc cgctgcttcc cgcgcggaca acgggccgtt gattgaagat ctcggtttga
     4201 tggaaaaaga ggacggtcat ttcgccgccg cggtgagcta cctgcaacag gcgcgctccc
     4261 tctacacgaa gcccgatgac attctgcgca ccgtcctgga gcaagccgat gcgttgatca
     4321 agtcgggcga taggaaggcg gcgctggccc tggtgcgcag cgtcgcgcaa ctggtgccgg
     4381 atgcatcggc gacggccctg ctgcgcaaga tcgagcagca actcaatccg ccgtcgctcg
     4441 ctccgacgcc atccccttcg cccctgtagc cgtcgccctg tgggcgaccc ggggaaaaac
     4501 atcgcgacgg ctaatcacgg tcgctctcgc gcttggcaga gcgaagcgtc tacaatccta
     4561 cggttgtggc gggttcggac gcttgggttc ggtccggcgc gattcgaggc gaaaaatcgt
     4621 ttcgccttcc ttcatcaact cgagactgtc gcgcgcgatg gtttcgagat aagtcggatc
     4681 cgatttcagc agtgtcacct gcttcgtctg gcgcgcgagg agcgtttgct ggtcggtcag
     4741 ttgcgcctgg agctggtcga tctccgcgcg attttgattc agctttttgt aaggcggcag
     4801 aaaaaagctg acgatgagca gccatacggc aataaaaaga agcacgcgca ggacgtggtt
     4861 catccgttgc cagatggtgg cctcgcgccg tgcgcgaaaa tcgccgtaac cggtggcgtc
     4921 cactagcgca tcttacctcc gaagaccgcc ttgtcaccca attcctccgc gatgcgcagc
     4981 agttggttgt acttcgccac ccggtcggtt cggcaaagcg agccggtctt gatctgcccg
     5041 gcgttggtcg cgaccgcgat gtcggcgatg gtggtgtcct ccgtctcgcc ggaacgatga
     5101 ctgatgaccg ccgtgtaatc gttttgcttc gcgagatcaa tcgtcgcgaa ggtctcggtc
     5161 agcgtgccga tctgattcac tttcacgaga atggaattgg ccactccgag gtcgatgcct
     5221 ttgcgcagga actgcacgtt ggtcacgaac aaatcgtcgc cgaccagttg cacctcggcg
     5281 ccgaggcggt cggtcagttt tttccagccg tcccaatcgt tctccgcgca gccgtcctcg
     5341 atcgagatga tgggaaagcg cgcgcaaagc gaggcgtagt aatcgaccag ttcggcggcc
     5401 gtccgcttgg aaccgtcgga tttcttgaag acgtaagcgt tctgttcctg gtcgaaaaac
     5461 tccgaggagg cgggatccag cgcgatgaaa atttgttcgc ctaacgagta gccggcttgc
     5521 tcgaccgcgg ccgcgatcga ctcgagcgcg tcctcgaccg acttgaggtt aggcgcgaaa
     5581 ccgccttcgt cgccaattgc cgtcgagagg ccgcggtcgt gcaggacctt cttgagcgca
     5641 tgaaaaactt ccgcgccgta acggagcgcc tcgggaaaag tgggcgcgcc tttcggcatg
     5701 atcatgaatt cctggaaatc aatcggcgca tcggagtgcg cgccgccgtt gaggatgttc
     5761 atcatcgg
//
LOCUS       DATMNS010000308         6884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_2146852, whole genome shotgun sequence.
ACCESSION   DATMNS010000308 DATMNS010000000
VERSION     DATMNS010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6884
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2146852"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(302..580)
                     /locus_tag="VNW28_10870"
     CDS             complement(302..580)
                     /locus_tag="VNW28_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10475.1"
                     /translation="MIHLIAAAAVYAHLMTGGWLTARYSLDDPNIVNLILAIFEPLAI
                     LSVIAYWIWQTPFLYRLLNIFCLAQLLIGIGFAIFILVFMLSWHPRMI"
     gene            complement(619..1542)
                     /gene="aroE"
                     /locus_tag="VNW28_10875"
     CDS             complement(619..1542)
                     /gene="aroE"
                     /locus_tag="VNW28_10875"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXA10476.1"
                     /translation="MNTIYTLADLRDWKKKTRDLSPPIRLGVVGNPVVHSFSPAMQNA
                     ALEESGLDLQYAPFEIAPDELESALRLLRELDFVGLNLTVPHKIAALALVDEADERAK
                     KIGAINTIIFRGGKSLGKNTDGPGFERAIREEFSVDLRDLRVLLLGAGGGAGRAIAFQ
                     CALENCERLVLVNRTFDKAQALAHALAGYFSGPRVLGPEARLEAIRWDDPSLRRQLGR
                     TDLVVNATTIGLQQSDPSPLPHGLIAPHLMVYDLLYKKTPTRLLAAAAEAGARGANGL
                     TMLLHQGALAFELWFDRPAPLVAMRRALDGK"
     gene            1654..3363
                     /locus_tag="VNW28_10880"
     CDS             1654..3363
                     /locus_tag="VNW28_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HXA10477.1"
                     /translation="MSDPANENSAPSDFIRDIVAEDVRSGKYTNIHTRFPPEPNGYLH
                     IGHTKAICLNFGIAHEFGGICNVRMDDTNPEKEETEYVDSIMADIHWLIDGWAEKNLG
                     LKQKSENRSAPFFASDYFEQIYAFAVQLVRKGKAYVCDLSAEQTDEYRRLGKESPDRD
                     RIVAENLDLLARMKAGEFPDGARTLRAKIDMQAPNMWLRDPVLYRIRHAAHHHTGDAW
                     SIYPLYDFAHCLSDYIEGITHSVCTLEFEVHRPLYDWLLEALDLPRPLPHQYEFARLN
                     LTYTVMSKRKLKQLVDDGLVKGWDDPRLPTISGMRRRGVTASVLRDFAYRIGITKYPS
                     MTEVDALEYAMRDEFNRTALRRLAVLHPLKVVITNYPEGKTEELEAVNNPEDPGAGTR
                     KVPFSRELYIESADFMETPPPKYFRLKPGGEVRLKYAYIIKCDEVIKDSSGQVTELRC
                     TADLESKSGGATSNRKIKGTIHWVSAAHAIAAEVRLYDRLFTVPEPDAEGDFKSFINP
                     HSLEVVTAKCEPSLGEAKAERRYQFERLGYFALDPDSRPGRLVFNRTITLRDTWAKEA
                     KKK"
     gene            complement(3555..4880)
                     /locus_tag="VNW28_10885"
     CDS             complement(3555..4880)
                     /locus_tag="VNW28_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HXA10478.1"
                     /translation="MSSAVRVRFAPSPTGFLHIGGARTALFNWLFAHHTGGTLVLRIE
                     DTDQARNTDEAAQAIYDGLRWLGLDWDEGPHAGGNFGPYFQSQRTEIYERHLRKLEAG
                     GHLFEENGAIRFRSPREHVVVDDVVCGKIDFDLTNPETHPDMTIRRPDGSWIFHFVNV
                     VDDLEMEISHVIRGEDHLSNTPKHIELYRALGAEPPRFAHIPLILNQDGSKMSKRDAG
                     SSLNTYIEEGFVPEAVRNYLCLLGWSPKDNREKIALEEVVRLFELEKIHRKNASFDLA
                     KCTWLNGEYARELSDDRFHALARVALVRAGIDTSKFPEEYVRAALETCKGKFRIFSEL
                     PAYCGFYFTDEFSYQPEGAAKHFIPENKPRLQALRDAFAALENFDATSVEAALKATAA
                     QLGVKVGVLVHPTRLAVTGSNAGPSLYHLLEILGKAKVLARLDRALAQF"
     gene            complement(4964..6076)
                     /locus_tag="VNW28_10890"
     CDS             complement(4964..6076)
                     /locus_tag="VNW28_10890"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HXA10479.1"
                     /translation="MAICLVAPSFALAEDRFQSFLEQTLAAAREKDHLPAVAALVQID
                     GKLAGEAALGVRALGHPEAVTTNDRWHIGSDTKAFTATLIARLVEQRVMRFDDTLADS
                     FPAFAAAMDPAYRNVTVTQLLSHTAGLPPLTDDKDLPAFIAVIKSANGVKAQREAIAR
                     KYLTMPPASKAGDFAYSNLGFIIAGAIAEARTGKTWEDLIRDRIFAPLGIKNAGFGPP
                     GTPGKLDQPWGHKDEAGKLVALDPASAEADNPPALGPAGTINIALKDWLLFAQDQLDG
                     EHGRGKLLKPETYRKLHTPVTGNYALGWGAKLGPDGVPLLLTHTGSNGFWLADIRIMP
                     RHDMIFLVVTNAGNDAANHAIKDIGTPLKDRLKPFE"
     gene            complement(6180..>6884)
                     /gene="tilS"
                     /locus_tag="VNW28_10895"
     CDS             complement(6180..>6884)
                     /gene="tilS"
                     /locus_tag="VNW28_10895"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXA10480.1"
                     /translation="AAADIRQIARAKKQSIETAARNERLAFFARVARRRRCPTIFLGH
                     NADDQVETFLLNLFRGAGGRGLGAMRARSSFGALGIARPLLGVWRAEIDRYVKEHRLK
                     FREDATNEKLDARRNRMRHQIIPYLERQLGRNIRATIWRTATVLAEEEDFLETLTPLG
                     LTKSKELDVAQLRKLAPALQRRVLRQWLSQRGVADLGFAVVEEVRGLLAGGAPARINL
                     SRGAHARRRAGKIFLE"
BASE COUNT         1325 a         2059 c         2252 g         1248 t
ORIGIN      
        1 gagctaattt tagaatcggt tcgtggggag cggggagagg ggcaccccga gaatgaaggg
       61 agcgcccgac gcaccgcggc gagcggggcc cgggcaatcg aaacccagtt cgaaggagag
      121 agcgccctgg tggaacaaca ggcgccgagc aaggggggac tgacccatcg cacccgcgtc
      181 gcccacaggg cgacggctac aggggcgggt ggaacgcgcg aggaaggcat tcaaccgcgc
      241 agttgtagcc gcttcgctgt gcgaagcgtg ggtatgtgtg tggagaccag aacgcgcgtc
      301 gctatatcat tctcggatgc caggagagca tgaaaacgag gatgaagatc gcgaacccga
      361 tgccgatgag cagctgcgcc aggcagaaga tgttgaggag ccgatagaga aaaggggttt
      421 gccaaatcca gtaagcgatg acgctcagaa tggcgagagg ttcgaagatg gccaggatga
      481 gattaacgat attgggatcg tcgaggctgt aacgagcggt caaccagcca ccggtcataa
      541 ggtgggcgta gacggcggcg gccgcgatca aatgaatcac ggaagctgac gcagctttca
      601 gcggtctcga catcgagttt acttgccgtc gagcgctcga cgcatcgcga cgagcggcgc
      661 ggggcgatcg aaccaaagct cgaaggcgag cgcgccctgg tgcagcaaca tcgtcaagcc
      721 attggccccg cgcgccccgg cctcggccgc ggccgcgagg agtcgcgtcg gcgttttctt
      781 gtagaggaga tcgtaaacca tcaggtgcgg cgcgatcagg ccgtgcggca gaggcgatgg
      841 atcggattgt tgcaggccga tcgtggtcgc gttgacgacc aaatcggtgc ggccgagttg
      901 ccggcggagc gatggatcgt cccagcggat cgcttcgagg cgcgcttcgg ggccgagcac
      961 gcgcggcccg ctgaagtagc cggccagcgc gtgggccagc gcctgcgctt tgtcgaacgt
     1021 gcggttcacg agcactagac gctcgcaatt ttccagcgcg cattggaaag caatggcgcg
     1081 gcccgctccc ccacccgcgc cgagcaggag cacccgcagg tcgcgcaggt cgaccgaaaa
     1141 ttcctcccgg atggcacgct cgaacccggg cccgtcggtg tttttgccta acgacttccc
     1201 gccgcggaag atgatggtgt tgatggcgcc gattttcttg gcgcgctcgt ccgcctcgtc
     1261 gacgagcgcg agcgccgcga tcttgtgcgg cacggtgagg ttaaggccga cgaagtcgag
     1321 ctcgcgcagg agacggagtg cggactccag ttcgtccggc gcgatttcga agggcgcata
     1381 ttgcaggtcg aggccactct cctccaacgc ggcgttttgc attgccggcg aaaacgaatg
     1441 cactaccgga ttacccacaa cgccgaggcg gatgggcggc gagaggtcgc gcgttttttt
     1501 cttccagtcg cgcagatcgg ccagggtgta gatggtgttc atgcgcgggc ggaacgggag
     1561 tttacagacg atgccatggg gtccctcgac cccgaatctt tgcggggctc gggatgacaa
     1621 gcgggcgcgc tgtagttatc gtcgcgccac cgcatgtctg atcccgcgaa cgagaattcc
     1681 gccccttccg atttcatccg cgatatcgtc gcagaagatg tgcggagcgg aaagtacacg
     1741 aatatccaca cccgcttccc accggagcct aacggctatc tgcacatcgg ccataccaag
     1801 gcgatctgcc tcaacttcgg catcgcgcac gagttcggcg ggatctgcaa cgtgcggatg
     1861 gacgacacca acccggagaa ggaggaaacg gaatacgtcg attccatcat ggccgacatc
     1921 cattggctga tcgacggctg ggcggagaag aatctcggac tgaagcagaa gtcagagaat
     1981 cgaagcgctc cctttttcgc gtccgactac ttcgaacaga tctacgcgtt tgccgtgcag
     2041 ctcgtgcgca agggcaaggc ctacgtttgc gacctgtcgg cggaacagac cgacgaatac
     2101 cggcggctcg gcaaggagag tccggaccgc gatcgcatcg tggcggagaa tctcgatctc
     2161 ctcgcccgga tgaaagcggg cgaattcccc gatggcgcgc gcactttgcg ggcgaagatc
     2221 gacatgcagg cgcctaacat gtggctgcgc gatccggtgc tctatcgcat ccggcacgcg
     2281 gcgcatcatc acaccggcga cgcgtggtcc atctatccac tctacgattt cgcgcattgc
     2341 ctgagcgatt acatcgaggg gatcacccac tcggtttgca cgctcgaatt cgaggtccac
     2401 cggccacttt acgattggct gctcgaggcg ctcgatctgc cgcggccgtt gccgcaccaa
     2461 tatgaattcg cgcgcctcaa cctcacctac acggtgatga gcaagcgcaa actcaaacag
     2521 ttggtcgacg acgggctggt caagggctgg gacgatcccc gcctgccgac gatctccggg
     2581 atgcgccgcc gcggggtgac ggcgagcgtg ctgcgcgatt tcgcctaccg aatcggcatt
     2641 acaaagtatc cgtcgatgac ggaggtggac gcgctcgagt atgcgatgcg cgacgagttc
     2701 aaccggacgg cgttgcgccg gctcgcggtg ctgcatccgc tcaaggtcgt gatcaccaat
     2761 tatcccgagg gaaaaaccga ggagctggaa gcggtgaaca acccggaaga tcccggcgcc
     2821 ggcacgcgca aggtgccgtt cagccgcgag ctttacatcg aaagcgccga cttcatggag
     2881 acgccgccgc ccaaatattt ccggctcaag ccgggcggcg aagtgcgttt gaagtatgcc
     2941 tacatcatca agtgcgacga ggtgatcaag gattcgtcag gccaggtcac ggagctgcgc
     3001 tgcacggccg atctggagag taaatcgggc ggggcgactt cgaaccgcaa gatcaagggc
     3061 acaatccact gggtgagcgc ggcgcacgcg atcgctgcgg aggtgaggct ttacgaccgg
     3121 ctctttaccg tgccggaacc ggacgctgag ggtgacttca aatcgttcat caatccgcat
     3181 tcactcgaag tggtgacggc aaagtgcgag ccgtcgttag gcgaggcaaa ggcggagcgg
     3241 cgttatcaat tcgaaaggct cggctatttc gcgctcgatc cggattcacg gcccggccgt
     3301 ctcgttttca atcgcacgat cacgctgcgc gacacctggg cgaaggaagc gaagaagaag
     3361 taggagctgc ggatcgcgga aacgtctggg gagcgcacgc gccctcgcgt gccccggtcg
     3421 gcgcctcgcc gaacggagga aggttcgagc tgtcgttgca atttcgcgag cacgctctgg
     3481 gttcgcggcg aggcgccgcg aacagcacgc gagggcgcgt gcgctccccg gatccgcgac
     3541 tccgcgcggg atgattaaaa ttgcgcgagc gcccgatcga ggcgggcgag gactttcgct
     3601 ttcccgagaa tttcgagcaa atgatagagg ctcggtccgg cgttgctgcc ggtgacggcc
     3661 agccgcgtcg ggtggacgag gacacccacc ttcaccccga gctgcgcggc ggtcgccttg
     3721 agcgctgcct ccaccgaagt cgcgtcgaag ttctcgagcg cggcgaacgc gtcgcgcagc
     3781 gcctgtagtc gcggcttgtt ttccgggatg aaatgtttcg ccgcgccttc gggctggtag
     3841 ctgaattcgt ccgtgaaata gaagccgcag taggcgggca gttcgctgaa gatcctgaac
     3901 ttccctttgc aggtctcgag cgcggcccgc acgtactcct ccgggaattt tgaggtatcg
     3961 atgccggccc tcacgagcgc gacccgcgcc agggcgtgaa aacggtcgtc cgataattcg
     4021 cgcgcgtact ccccgttgag ccaggtgcat ttggcgagat cgaacgacgc gttcttgcga
     4081 tgaatcttct ccagctcaaa cagcctgacg acttcttcga gcgcgatctt ttcccggttg
     4141 tccttcggcg accagccgag gagacagaga taattgcgga ccgcttcggg cacgaaaccc
     4201 tcctcgatat aggtgttgag gctcgacccg gcgtcgcgct tgctcatttt cgacccgtcc
     4261 tgattcagga tgagcgggat gtgcgcgaag cgcggcggct ccgcgccgag cgcgcggtaa
     4321 agctcgatgt gtttcggcgt gttggagaga tgatcctcgc cgcgaatgac gtggctgatt
     4381 tccatctcga ggtcgtcgac cacgttaacg aaatgaaaaa tccacgagcc atcgggccgg
     4441 cggatggtca tgtcggggtg cgtctcggga ttcgtcaggt cgaaatcgat ctttccgcag
     4501 acgacgtcgt cgaccacgac atgttcgcgc ggcgagcgga aacggatcgc gccgttttcc
     4561 tcgaaaaggt ggccgccggc ttccagtttc cgaagatgcc gctcgtagat ctcggtgcgc
     4621 tgactttgga aatagggccc aaagtttccg ccggcgtgcg gaccttcatc ccaatcgagc
     4681 ccgagccagc gcagcccgtc gtagatcgcc tgcgcggctt cgtcggtgtt gcgggcctga
     4741 tccgtgtcct cgatccggag gacgagggtg ccgccggtgt ggtgcgcgaa aagccagttg
     4801 aagagcgcgg tgcgcgcgcc gccgatgtgc agaaagccgg tgggtgatgg cgcaaaacga
     4861 acgcggacag cggaactcat ttcttaggat ttcaatcagg aaagcaggaa agcaggaaga
     4921 aagcgccgat tcattaagtg agctctaaat aatcggcgcc gccttactcg aacggcttga
     4981 ggcggtcttt caaaggtgtg ccgatgtctt tgatggcgtg gttggcggcg tcgttgccgg
     5041 cgttcgtgac gacgaggaag atcatgtcgt gccggggcat gatgcggatg tcggcgagcc
     5101 agaatccgtt gctcccggta tgcgtcaaaa gcagaggcac accatcgggg ccgagcttcg
     5161 cgccccagcc gagcgcgtag tttcccgtca ccggcgtgtg caatttgcga taggtttccg
     5221 gtttcagaag tttgccgcgg ccgtgctcgc cgtcgagctg atcctgggcg aaaagcagcc
     5281 aatccttgag cgcgatattg atcgtgccgg ccgggccaag cgctggcgga ttgtcggcct
     5341 cggcgctcgc gggatcgagc gcgacgagtt tgcccgcctc gtctttatga ccccagggtt
     5401 gatcgagttt gcccggcgtg cccggcgggc cgaaacccgc gttcttgatg ccgagcggcg
     5461 cgaagatccg gtcgcggata aggtcttccc acgtcttgcc ggtgcgcgcc tcggcgatcg
     5521 cgccggcaat gatgaagccg agattggaat atgcgaagtc gccggctttg gaggctggcg
     5581 gcatcgtcaa atatttgcgc gcgatcgcct cgcgctgcgc tttcaccccg ttcgccgact
     5641 tgataacggc gatgaacgca ggcaggtcct tgtcatcggt cagcgggggc agaccggccg
     5701 tgtgactcaa aagctgggtc acggtcacat ttcgataggc ggggtccatt gccgccgcga
     5761 aagcagggaa cgagtccgcg agggtatcgt cgaagcgcat gacgcgttgt tcgaccaacc
     5821 gcgcgatgag cgtggcggtg aacgccttgg tgtcggagcc gatgtgccag cgatcgttgg
     5881 tcgtcactgc ctcggggtgg ccgagcgcgc gcacgccgag cgccgcctcg cccgcaagct
     5941 tcccgtcgat ctgaaccagc gcggcgaccg cgggcagatg atccttctcg cgggccgccg
     6001 ccagcgtttg ctcaagaaac gactgaaagc gatcctcggc caacgcgaag gacggcgcga
     6061 cgagacagat cgccattaga accgtgcgaa ggacaagacc gactttcatc atctcccttc
     6121 gtcactcgca cgcggcgatt tgccaagagc caaatttccc gatctcgtgg cccgcgcttt
     6181 cactcgagaa aaattttgcc ggcccgacgc cgggcatgcg cgccgcgaga gagattgatc
     6241 ctggccggcg cgccgcccgc gagcaatccc ctcacctcct cgacaacggc aaagcccaga
     6301 tccgcgacgc cgcgctgcga aagccactgt cggaggacgc ggcgctgcaa ggccggcgca
     6361 agtttgcgaa gttgcgcgac atccagctct ttgcttttcg ttaggccgag cggcgtcagc
     6421 gtttccagaa agtcctcctc ctccgccagg acggtggcgg tgcgccagat cgtcgcccga
     6481 atattccgcc ccagctgcct ttccagataa ggaatgattt ggtgccgcat gcgattgcgc
     6541 cgcgcgtcca gcttctcgtt cgtcgcgtcc tcgcgaaact tcaacctgtg ctccttcaca
     6601 tagcgatcga tttcggctcg ccacacgccg agcagcgggc gcgcgatccc gagtgcacca
     6661 aagctcgacc tggcccgcat tgcgccgagt ccccgcccgc cggcgccgcg aaaaaggttg
     6721 agcaagaagg tttcgacctg gtcatcggcg ttatgcccga ggaagatcgt cgggcaacgg
     6781 cgccggcgcg caacgcgcgc gaagaaagcg agccgttcat tgcgcgccgc ggtttcaatc
     6841 gattgttttt ttgcgcgcgc aatttggcgg atgtctgcgg ccgc
//
LOCUS       DATMNS010000309         3219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3843401, whole genome shotgun sequence.
ACCESSION   DATMNS010000309 DATMNS010000000
VERSION     DATMNS010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3843401"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(270..686)
                     /locus_tag="VNW28_10900"
     CDS             complement(270..686)
                     /locus_tag="VNW28_10900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXA10481.1"
                     /translation="MSNGLLNILLVEDDEVDVMNVKRAFAQNNIRNPLFVARDGVVAL
                     EKLRSGEIPNERRVVLLDLNMPRMNGIEFLREVRNDPALRSTTVVVLTTSKDEQDRID
                     AYNLNVAGYLVKPVTFTKFCEVMVALDKYWSLVEFL"
     gene            complement(686..2038)
                     /locus_tag="VNW28_10905"
     CDS             complement(686..2038)
                     /locus_tag="VNW28_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXA10482.1"
                     /translation="MISDIRGGATSERGNLTGSANMNRGSIEERGAQEELRRSEERFE
                     LLVRSVKDYAILMLDPEGRVVSWNEGAERIKGYPANEILGKSFTTFYPREAVDSGFPQ
                     LELDVAKREGRFEDEGWRVRKDGSRFWANVIITALRGPDDQLVGFAKVTRDLTARREA
                     EAQARRLAAEQAAHAEAAARSEELAQLNRRLREQAAELQVALADTKDARDAAERAAAA
                     TSEAYRELDQFAYVASHDLKAPLRGIANLAQWLQDDMGEQLTGDSKEHMRLLQGRVRR
                     MEALIDGILAYSRAGRRLTAPEPVGTGALVRDVIELLAPPAEVRIQMPDQFPTIEAER
                     VPLQQVFMNLIGNAIKFTRAVRPDAVIRIAWRDVGDGFEFTISDNGSGIAPEYHDRIW
                     GIFQTLAARDKVEGTGIGLSVVQKIVESRGGKVSLESAPGEGATFRFVWAKTLRPGFS
                     "
     gene            2807..3118
                     /locus_tag="VNW28_10910"
     CDS             2807..3118
                     /locus_tag="VNW28_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10483.1"
                     /translation="MKKAKKRAKSKTSSKRRVKSKTSSKRVGAKSKTRKQIKVKPPTK
                     SKKSILPKTPKLKQVAKEAAVAAGLAAVGTALSAMKPQRKGAEEAAGSGAEKKVDTSK
                     N"
BASE COUNT          632 a          994 c          940 g          653 t
ORIGIN      
        1 tagaacaacg aaagctcggg cacatcgggg agatagtagt cgaggagaac gcagtcgtag
       61 gcggagctgc cgattagctt aagggcctca gccgcggacc cagcttcgtc gaccgcgact
      121 gacatgccgg agttatgcag gcaacgccga acggcaagcc gatcggtctc atcatcatca
      181 acgatgagaa ttcgaagtcg gtaggtgcca ttctgtgacg ctaatggaat gctttcggtt
      241 gtcgagccaa tgttctccat cacgcatcct cacaggaact caacaagaga ccagtacttg
      301 tccagcgcta ccatgacctc gcaaaacttc gtgaaagtga ccggcttcac gaggtatccc
      361 gcgacgttca gattgtaggc atcgatcctg tcctgttcgt ccttagaggt ggtcagcacg
      421 accacggtcg tcgagcgtag ggcaggatcg ttccgcacct cgcgaaggaa ctcgatcccg
      481 ttcatccggg gcatgttgag gtcgagcagg acgacccgcc gctcgttcgg tatctcgccg
      541 ctgcgcagtt tctccagcgc cacgacccca tcgcgcgcga cgaatagagg attcctgatg
      601 ttgttttggg cgaacgcacg cttcacgttc atcacatcga cctcgtcgtc ttcgacgagt
      661 aaaatattta ggagtccgtt gctcattagg agaaccccgg ccttaatgtc tttgcccaaa
      721 cgaaccggaa cgtcgcgccc tcgcctggcg cagactcgag tgaaaccttg ccgccgcgac
      781 tttcgacgat cttctgcacg actgaaagtc cgatgccagt accctccacc ttgtcgcgcg
      841 ccgcgagcgt ctggaagata ccccagatcc gatcatgata ttcgggggct attcccgacc
      901 cgttgtcgct gatcgtgaac tcaaaaccgt ctccaacgtc gcgccacgcg atgcggatca
      961 ccgcatcggg gcgcacggct cgggtaaact tgatcgcatt gccgatgagg ttcatgaaga
     1021 cctgctgcag cggcacgcgt tccgcctcga tggtcgggaa ctggtcgggc atctgtatcc
     1081 tcacctccgc gggtggcgcg aggagttcga taacgtcccg tacaagcgcg ccggtcccca
     1141 ccggctccgg cgctgtgagt ctgcggcctg cgcgtgagta ggcaagaatg ccgtcgatca
     1201 atgcttccat ccggcgcacg cggccctgga ggaggcgcat gtgctctttg gaatcacccg
     1261 tgagctgctc ccccatgtcg tcctggagcc attgcgccag gttcgcgatc ccacgcagcg
     1321 gcgccttcag gtcgtgcgaa gcaacgtaag cgaactgatc gagctcacga tatgcttcgg
     1381 aggtggccgc ggcggcgcgc tcggcggcgt cgcgagcgtc cttggtgtcg gccaatgcca
     1441 cctgcagctc ggcggcttgc tcccggagcc gacgattgag ctgcgccagc tcctcactgc
     1501 gggcggccgc ttcggcatga gcggcctgct ccgccgcgag acggcgagcc tgcgcctcgg
     1561 cctcgcgccg cgccgtcagg tcgcgcgtga ctttggcaaa gccgaccagt tgatcgtcgg
     1621 ggccgcgcag tgcggtgatg attacgttgg cccagaaacg ggagccatcc ttgcgcaccc
     1681 gccagccttc gtcttcgaaa cgaccttcgc gcttcgcgac gtccagttcc agttgtggaa
     1741 agcccgaatc aacagcctct cttgggtaaa aggtcgtgaa agatttgccg aggatttcat
     1801 tggcggggta gcctttgatc cgctcggctc cctcgttcca actcacaacg cggccctcgg
     1861 gatcgagcat gaggatggcg taatccttca cgctccgaac cagcagctcg aaacgctctt
     1921 cgctccggcg cagttcttcc tgtgcgccac gctcctcgat cgaaccgcgg ttcatgttag
     1981 cggagccggt caagttgcct ctctcggatg tagctcctcc ccggatgtcg ctgatcacga
     2041 gtttcgtgtt gcatcaggga gtggacacgt gcggcccctc gcagctcccg gtccggtggc
     2101 gcgcgtccgg gcgtgcccat ccctgccgga aatgggccac ccggcgtgtg tttgcggctc
     2161 cgacagaggc gttcacgcca tgccagttgc gcgggctgct ttctttgcga cgcgcttaag
     2221 tcccgcccct gtcctccgaa tagtacgttt cgctgccttt acgactttct ttgttttgga
     2281 ttttctgcgt ttagccattc tatttcctcc tgttcgctac ctcgtcggcc ctagcccgcg
     2341 gctttggatt aggatttgga agctgtcgcg atggcgcggg cctcgtccgc ttgttgtcgc
     2401 cagtgcttcg ggtcgttgaa acgaccggca gtcatcgtca cacccacact gcactcgctg
     2461 caacgcaacg tgtatgtgct gtgacttgtt ccgactgcta ccggtgcgac tatccgtacg
     2521 tcggcaccac gtcgcgctcg cgccggcttg ccgcaccgtt ctgcggcgcc ggcggcgcag
     2581 tgacccgatc gcggtcgatt gcaaatcgat tatactccgg atcgcgcctg ctcggcgacg
     2641 ggggaccaaa gcgagcccgc ctaaggcaaa accgatcgga tgacttgcct tattttagga
     2701 atcacattcc gatagcggca gagagtcagg aaatccatcc cgactgcttg ccagcaacag
     2761 gattgaggcc caatctgaaa ctaagttgaa tcgaataggg aaaatcatga aaaaggcaaa
     2821 gaagcgcgcc aaatccaaga cttcatcgaa acgacgggtt aagtcgaaga cgagttcaaa
     2881 gcgtgtcggc gccaaatcga agactcgcaa acagataaag gtcaagccgc cgacgaaatc
     2941 gaaaaaatcc atcctcccta agacgccgaa gctcaagcaa gtagctaagg aagcggccgt
     3001 cgccgcaggg ttggctgcag tcggcactgc tctgtccgca atgaaacccc aacgaaaggg
     3061 ggccgaggag gctgctggaa gtggggccga aaagaaggtc gacacatcga agaattgaac
     3121 gagagagcgc caacgcctct agggaacttt gggtgtcggt aattgttcaa cggccggacc
     3181 cgtggtcctg cacgagttcc gcgaaccccg cccgggcag
//
LOCUS       DATMNS010000310         6112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_1428303, whole genome shotgun sequence.
ACCESSION   DATMNS010000310 DATMNS010000000
VERSION     DATMNS010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428303"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VNW28_10915"
     CDS             <1..476
                     /locus_tag="VNW28_10915"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006977745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXA10484.1"
                     /translation="GADEDRQGEPFVGRAGQLLTKIIAAMGFSRDEVYIANVLKCRPD
                     MPAGEPGNRKPTPDEMETCLPYLLEQIEIIGPRVLVALGATAVEGLLKIREPMGKLRG
                     RWQTYRDTPLMITFHPSYLLRNQSNTEKRKVWEDMLLVLERLEHPITEKQRNYFR"
     gene            complement(553..1374)
                     /locus_tag="VNW28_10920"
     CDS             complement(553..1374)
                     /locus_tag="VNW28_10920"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXA10485.1"
                     /translation="MSETPYRSIAVAATFSPRFHQVLAEANRVRTRFGSALSMIYVGE
                     CDATTTRKFAEAIEQLNLPADSVVHYEEGDPASGILAAARKLQVQLLVAGALEKEAIH
                     RQFLGDVARRLVREATCSVMLFTRPELEPKPLRHIVFIAEYAEHAQAALRRTLRLAEG
                     EHCERLYVIRSYTSFDKARAARRSKTSKGQARTLEEEEIALQEFVLAAGPTEVPIEAR
                     CIRGNTGFATSNFVQAVEADLLVVPLETKSEPTPKLPSRISWVTDVIPCNLWIVR"
     gene            1472..1951
                     /gene="coaD"
                     /locus_tag="VNW28_10925"
     CDS             1472..1951
                     /gene="coaD"
                     /locus_tag="VNW28_10925"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXA10486.1"
                     /translation="MRRAIYPGSFDPVTNGHLDVIERARKLFDEVIVAVAHNDQKQPL
                     FSLAERLDFLGQVTASLAGVKVAPLDGLLVEFAVRQNAHAVVRGLRAVSDFEFEFQMA
                     LMNRKLEATVETIFLMPKEEYTYLSSRIVKEIARLGGDVGAFVPPCVLESFARKLRR"
     gene            2016..2468
                     /locus_tag="VNW28_10930"
     CDS             2016..2468
                     /locus_tag="VNW28_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10487.1"
                     /translation="MKIHLRQIPAEGLHLEGEEDCPIPQLDPEEARCAGPLSYALDAG
                     VSEGALWVNGKLRQPVELRCVRCLESFPFDIEVRDFTIHTELAGPEAVDLTPFMREDI
                     LLNLPAYPHCDREGGRVCPVPEASKQADAKAVLEERPPDWSELDRLEL"
     gene            2595..2777
                     /gene="rpmF"
                     /locus_tag="VNW28_10935"
     CDS             2595..2777
                     /gene="rpmF"
                     /locus_tag="VNW28_10935"
                     /inference="COORDINATES: protein motif:HMM:NF013908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXA10488.1"
                     /translation="MGVPKRKTSKMRLRTRKASHRWQPPQLNKCPQCGSTVKSHTACA
                     SCGYYKGRQVLTVDAG"
     gene            2918..3949
                     /gene="plsX"
                     /locus_tag="VNW28_10940"
     CDS             2918..3949
                     /gene="plsX"
                     /locus_tag="VNW28_10940"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXA10489.1"
                     /translation="MKIALDAMGGDFGPPNLVGGAVLALREYPQIDQLFLVGDTPQIE
                     AELQKRECNDRRIEIVHSTQVVAMTDGAVQSVRRKKDSSVSRAVDLVKKGEAAAIVSA
                     GHTGAAVAATTIKLRTLPGIDRPGIAAVFPSETNIFVLIDAGANSDARPEHLLQYGIM
                     GSVYSRHVLGYKNPSIGLMSIGGEDVKGTDLTKEVFKMLKCSALNFRGNVEGHDLFAH
                     PVEVVVCDGFVGNVILKTCESVGDAIFKWLKHELTKNKLRMAGAYLAQSAFRAIRKKV
                     NYEEYGGSPLLGVNGICIIAHGASTPLAIKNALRVATEAIEQQVNPHIVEEIRRYNET
                     NAPLEPAVR"
     gene            3912..4937
                     /locus_tag="VNW28_10945"
     CDS             3912..4937
                     /locus_tag="VNW28_10945"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HXA10490.1"
                     /translation="MKRTPRSNPRSAKPLRTVSIVGTGSYVPEKILTNADLSKIVATD
                     DKWITTRTGIKERRVAADDEYTSDMGAKAALAAMEQAEIKGEEIDLILVATATPDMVF
                     PATACFIQTKIGATHAACLDISAACAGFLFAVEIAQQFIACHTYDTVLVVGAEKLTTI
                     TDWTERNTCVLFGDGAGAAILRHRDGGSHGLISTHLASDGNFADILWMPGGGTRHPIT
                     RENADDHLQTIKMSGKEVYKQAVIAMVDAAKKALAKAGLTIDDIACVIPHQANVRIIE
                     AIADRLKISVDKFFVNLERYGNTSAAAVAIALDEANRSGRIKAGDYVLLVVFGGGLTW
                     AGSVIEW"
     gene            4998..5864
                     /locus_tag="VNW28_10950"
     CDS             4998..5864
                     /locus_tag="VNW28_10950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXA10491.1"
                     /translation="MLIDTHAHLDYPDFADDFRAVLARANEAGVTRIITIGTSLASSR
                     RAVELAEKHPNVFAVIGVHPTNAEQAPDDIIAPLRELAKSPRVAALGETGLDYHRLPS
                     AVVAGERKSQVISGALQAETDDEIESEIHDGALKSKQASLFQQQLDLAVELRLNVVIH
                     QRDAWADTLEIMREYGRKVSGVFHCFGGTKEQAEEVIALGHLVSFTGIVTFKNGASVR
                     DVAAALSLGEFMVETDCPYLAPVPFRGKRCEPAHTRLVAESIATARGVSLEEVARATT
                     QTAEEFFRLSGA"
     gene            5861..>6112
                     /locus_tag="VNW28_10955"
     CDS             5861..>6112
                     /locus_tag="VNW28_10955"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HXA10492.1"
                     /translation="MRRRLPFCLHLVGATALLSLGASCASVDRRHQVIISIPEQRMAL
                     LEDGAPLATYPVSTSKFGLGDLPGSRGTPLGTLEVAQKIG"
BASE COUNT         1228 a         1876 c         1848 g         1160 t
ORIGIN      
        1 ccggcgcgga tgaagatcgg cagggcgaac cgttcgtcgg gcgcgccggt caattgctca
       61 ccaaaatcat cgctgcgatg ggtttcagcc gcgacgaggt ttacatcgct aacgttctca
      121 agtgccggcc cgacatgccc gccggtgagc ctggcaatcg caagccgact ccggatgaaa
      181 tggaaacctg ccttccctat ctgctcgagc agatcgaaat catcggaccc agggtcctgg
      241 tcgcactcgg ggcaacagcg gtcgaagggc tcctgaaaat tcgcgaacca atggggaagt
      301 tgcgcggccg ctggcagacc tatcgcgaca cgcctttgat gatcacgttt catccctcgt
      361 atttgctgcg gaaccagagc aacacggaga agcgcaaggt ctgggaagac atgctcctcg
      421 tcctcgagcg cctcgagcac ccgatcacgg agaaacagcg caactacttt cgttaggcgg
      481 ggcgccggtt ggaaggcgat ttgccatcga ctctgctccg aggagcgcga cgcggggtga
      541 gaattccgac gcttagcgca cgatccagag gttgcacgga atcacatccg tgacccagga
      601 aatccggctc ggcagtttgg gcgtcggctc ggacttcgtc tccaacggaa cgacgagcag
      661 atcggcctcg accgcctgga caaaattgga ggtggcaaac ccggtattgc cgcgaatgca
      721 gcgcgcctcg atcggaactt cggtcggccc ggcggcaagg acgaattcct gtagcgcaat
      781 ttcctcttct tccaaagtgc gagcttgacc tttggaggtc ttgctccgcc gcgcggcgcg
      841 tgccttgtcg aagctagtgt agctgcggat cacgtagagg cgctcgcaat gttcgccttc
      901 cgcgagccgc aaagtgcggc gcaacgcggc ttgggcgtgc tcggcatact ccgcgatgaa
      961 aacaatgtga cggagcggct tcggttcgag ttccggacgg gtgaaaagca tgaccgaaca
     1021 ggtggcttcg cgcaccagcc ggcgcgcgac gtcgcccaga aattgccgat ggatcgcttc
     1081 cttctccagt gcgccggcga ccagaagttg gacctgcaat ttgcgcgccg cggcaaggat
     1141 gccgctcgcg gggtcgcctt cttcataatg cacaacggaa tcggcgggca ggttcagctg
     1201 ctcgatcgcc tccgcgaatt tccgcgtcgt cgtggcatcg cattctccga cgtagatcat
     1261 gctcagggcg gagccaaaac gggtgcgcac tcggttggcc tcggccagga cctggtggaa
     1321 acggggcgaa aacgtcgccg ccaccgcaat ggaccgatag ggagtttcgc tcatggttct
     1381 cccgctactt tggagcgtgg ccagccaacg gcaaccaggg actcgaccgt gctgcgttga
     1441 aattcgccgt tcctctgcga atttccggcc catgcgacgc gcgatttatc ccggcagctt
     1501 cgacccggta accaacggtc atcttgatgt gatcgagcgc gcgcgaaaac tttttgatga
     1561 ggtgatcgtc gccgtggcgc acaacgacca gaagcaaccg ctcttcagcc tcgcagagcg
     1621 gctcgatttc ctgggccagg ttacggcgtc actcgccgga gtgaaggtgg cgccgttgga
     1681 cggattgctg gtcgaatttg cggtccgtca gaacgcgcac gcggtggtgc gcggattgcg
     1741 cgcggtctcg gatttcgagt tcgaattcca gatggcgctg atgaatcgta aactggaagc
     1801 gacggtggaa accatctttc tgatgccgaa agaggagtac acttatctca gctcgcggat
     1861 cgtgaaggag attgcgcggc tcggcgggga tgtgggggcg tttgtcccgc cctgtgtgtt
     1921 ggaaagtttt gcacggaagt tgcggcgatg aaattccgaa aggatgcgag tgaactccga
     1981 aaggtttcgc gagtgaattc cgagacatta cacgagtgaa aattcatttg cgccaaatac
     2041 cagccgaggg cctgcatctg gaaggcgagg aagattgtcc gattccgcag cttgacccgg
     2101 aggaggcgcg ttgcgcggga cctctgagct acgcactcga tgcgggcgtt tccgagggcg
     2161 cgctctgggt aaatgggaag ctgaggcagc cggtcgaact gcgttgcgtg cgctgcctgg
     2221 aatcgttccc tttcgacatc gaggtgcggg atttcaccat tcacaccgag ctcgccggac
     2281 ccgaagccgt cgacctgacg cccttcatgc gggaagacat cctgctcaat ctgccggcct
     2341 acccgcattg cgatcgcgaa ggcggtcggg tctgcccggt gccggaggcg agcaagcagg
     2401 cagacgcgaa ggcggtgctc gaggaacgcc cgccggactg gagcgagctg gatcggttgg
     2461 aattgtagac gcttcgctct gcgacgcgcg gccggaacgt cttttctact tgagccaccc
     2521 ggctctccac gtttcgcagt acgaaccgtc tacatttgtg ttttccggat ttcctgcttt
     2581 cctgagtcgc tcgtatggga gttccaaaac gcaaaacttc gaagatgcgg ctgcgaacgc
     2641 gcaaagcatc gcatcgctgg cagccgccgc agctcaacaa gtgcccgcaa tgcggcagca
     2701 cggtgaaatc gcacaccgcc tgcgcttcct gcggatatta taaggggcgg caggtgctga
     2761 cggtggacgc tggctagggt gggtcagggg cttctgtgcc ccgatcttcg ctcggcgctc
     2821 ttcaatccac tctcgattct cgcggcacca aggccgctcc caccttgaga tttttcgttt
     2881 tgcattcgcc aactgaaaat cgcagattgg cccctcgatg aaaatcgctc tggacgccat
     2941 gggtggcgat ttcgggccac cgaacctggt cggcggcgca gtcctggcac tgcgcgagta
     3001 tccgcaaatt gaccaactct tcctcgtcgg cgacaccccg cagatcgagg ccgaattgca
     3061 gaagcgggag tgcaacgatc gccggatcga gatcgttcat tcgacccagg tcgtcgcgat
     3121 gaccgacggc gcggtccaat ccgttaggcg gaaaaaagat tcatccgtca gtcgggcggt
     3181 cgaccttgtg aagaagggcg aagcagccgc gatcgtcagc gcggggcaca ccggcgccgc
     3241 ggtcgccgcg accacgatca agctgcgcac tctccccgga atcgatcggc cgggcatcgc
     3301 cgccgttttt ccctccgaaa cgaatatctt tgtcctgatc gatgctggtg cgaacagcga
     3361 cgcgcgaccg gaacacttgt tgcagtacgg aatcatgggc tccgtctatt cgcgccacgt
     3421 tctgggttac aaaaatccct cgatcggcct gatgtcgatc ggcggcgagg acgtcaaagg
     3481 caccgacctg acgaaagagg tcttcaaaat gttgaagtgc agcgcgctca atttccgggg
     3541 caatgtcgag gggcacgatc tctttgccca tcccgtcgag gtcgtggtct gcgacggctt
     3601 tgttggcaac gttattctca aaacctgcga atcagtcggc gacgcaattt tcaagtggct
     3661 caagcatgag ttgacgaaaa acaaattgcg catggccggc gcctacctcg cgcaaagcgc
     3721 ctttcgcgcg attcggaaga aagtgaacta cgaagaatat ggtggttccc cactcctcgg
     3781 ggtgaacggg atctgcatta tcgcgcacgg cgccagcacc ccgctggcga tcaagaatgc
     3841 gttgcgcgtc gcgaccgagg cgattgaaca gcaggtcaac ccacatatcg tcgaggaaat
     3901 ccgccgctat aatgaaacga acgccccgct cgaacccgcg gtccgctaag ccacttcgca
     3961 ccgtttcgat tgtcggcacc ggcagctacg tgccggaaaa aatcctgacc aacgccgatc
     4021 tctccaagat cgtggcgacc gacgacaagt ggatcactac ccgcaccggg atcaaggaac
     4081 gccgcgtcgc ggcggacgac gaatacacca gcgacatggg tgccaaagca gcccttgccg
     4141 cgatggagca agctgagatc aagggcgagg agattgatct cattctggtc gcgaccgcca
     4201 cgccggacat ggtctttccc gctaccgcct gtttcattca aacgaagatc ggtgcaaccc
     4261 atgcggcctg tctcgacatc tcggcggcct gtgccggttt ccttttcgcg gttgagattg
     4321 cgcagcaatt catcgcctgc cacacctacg acaccgtgct cgttgtcggc gcagaaaaac
     4381 tgacgaccat caccgattgg acggaacgca acacctgcgt gctcttcggc gatggtgccg
     4441 gcgcggccat cctgcgccac cgcgacggag gcagtcacgg cctcatcagt acccacctcg
     4501 cgagcgacgg aaactttgcc gatatcctct ggatgcccgg cggtggcaca cgccatccca
     4561 tcacccgcga gaacgccgac gatcacctcc aaacaattaa aatgtcgggc aaggaggttt
     4621 acaaacaggc ggtcatcgcg atggtcgacg ccgcaaaaaa ggccctcgcg aaagccggcc
     4681 tcacgatcga cgatatcgcc tgtgtcatcc ctcatcaggc caacgtccgg atcatcgagg
     4741 cgattgccga ccggctgaaa atttcggtcg acaaattttt tgtgaacctc gagcgctacg
     4801 gaaacacctc cgccgcggcg gtcgccatcg cgctcgacga agcgaaccgg agcggccgaa
     4861 tcaaagccgg cgattatgtg ctgctggtcg tcttcggagg cggcctgacc tgggcgggct
     4921 cggtcatcga atggtagggc cggtttccgc tcgctcgaaa attactggca agcgggaagc
     4981 ttgcgctacc ttctccgatg ctaatcgaca ctcacgccca tctcgactac ccggattttg
     5041 ccgacgattt tagagcggtc ctcgcgcggg cgaacgaagc cggggtcacg cgcatcatca
     5101 ccatcgggac ctcgctcgcg agcagccggc gcgcagtcga gctcgccgaa aagcatccga
     5161 acgtttttgc cgtcatcggg gttcatccaa ccaacgccga gcaagctccg gacgacatca
     5221 tcgccccgct gcgcgagctg gcgaaaagcc cgcgggtcgc ggccctcggc gagaccggac
     5281 tcgactacca tcgcctgccc agcgcggtcg tggcgggcga gcggaaatcg caggtgattt
     5341 cgggcgcctt gcaggcggaa accgacgacg agatcgagag cgagatccac gatggcgcgc
     5401 tcaagagcaa gcaggcgagt ttatttcagc agcaactcga tctcgcagtc gaactgcggc
     5461 ttaacgtggt catccatcag cgcgacgcgt gggccgacac gctcgaaatc atgcgcgaat
     5521 acggccgcaa agtgagcggc gtgtttcact gctttggcgg cacgaaggaa caagccgaag
     5581 aggtgattgc gctcggtcac ctcgtttctt tcaccggcat cgtgaccttc aagaatggtg
     5641 cctccgtccg cgacgtcgcc gcggcccttt cgttaggcga attcatggtc gaaaccgatt
     5701 gtccctatct cgcgcccgtc ccctttcgcg ggaaacgctg cgaaccggcc cacacccggc
     5761 tggtcgccga gagcatcgcc accgcccgtg gcgtcagcct cgaagaagtg gcgcgcgcca
     5821 cgacccagac cgcggaagag tttttccgtc tctccggcgc atgagacggc gcctgccttt
     5881 ctgccttcac ctcgtcggcg cgaccgcgct cctctcgtta ggcgcttcct gcgcttcggt
     5941 cgatcggcgc catcaggtga tcatcagcat tccggaacaa cgcatggcgc tgctcgaaga
     6001 cggcgctccc ctcgccacct atccggtttc gacgtcgaag tttggactcg gcgaccttcc
     6061 cgggagccgc ggaacgccgc tcggcacgct cgaggtggcg caaaaaattg gc
//
LOCUS       DATMNS010000311         2554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3215912, whole genome shotgun sequence.
ACCESSION   DATMNS010000311 DATMNS010000000
VERSION     DATMNS010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3215912"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /gene="sucA"
                     /locus_tag="VNW28_10960"
     CDS             <1..1392
                     /gene="sucA"
                     /locus_tag="VNW28_10960"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXA10493.1"
                     /translation="EPRFTQPLMYSAIEDQPPISDIFFGHLVKFGDITPDEVRAFRAT
                     FEERLNDALQKSKVATKTIVPALRKSMSCPALLDPFESAVPLEKLREIGAAITREPEK
                     IELNPKIKRWLQSRHDMIEGKAPLDWSTAEALALGSLLESGYPVRLSGQDSRRGTFTQ
                     RHSVLYDMKTRERYTPLNNIKPGQAKFCVYNSPLSEYAVLGFDFGYTLDFPDMLVLWE
                     AQFGDFANGAQIVIDQYVACSETKWGETSGITLLLPHGYHGQGPEHSSARLERYLQAC
                     AEDNIQVANPSTPASYFHILRRQALRKIKKPLVLMTPKGMLRDPRCTSPIENFSKGRF
                     EEILPDTIAPKEAKRIVFCSGKIYFDLVDHLKAENIRDAAIVRVEQLYPLHEKKLRDA
                     VASYPKLEKIVWCQEESANMGAWNFIEPRLRKLFGRDILYAGRDASASTATGATAIHE
                     LEQRELIKQAFTL"
     gene            1410..>2554
                     /gene="odhB"
                     /locus_tag="VNW28_10965"
     CDS             1410..>2554
                     /gene="odhB"
                     /locus_tag="VNW28_10965"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF004309.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HXA10494.1"
                     /translation="MPIEVKVPSVGESITSGILGVWNKPDGSYVKAGEPIFEVETDKV
                     TSEVVAESSGQLKHLAKAGDTVKIGQIVASIDEKAAAPAEAAPAKVEPKKETANGTTE
                     KTSRLEASAPVAASAARGKNADDLSPAVRYQAEEKGIDPASIKGTGKEGRILKGDVLA
                     AGTSADSSKAAAVPAPQPLGERTSRRKMTPLRQKIAARLLSAQQETAHLTTFNEVDLT
                     NIMALRTKFQDRFVKKHEVKLGFMSFFVKAVLHALQVVPAVNARIDGEEVVQNHFYDI
                     GVAISTEKGLLVPVLRDADKLSFAGVEKAIAAYAKKARDGKITLSDLEGGVFTITNGG
                     VFGSLLSTPILNPPQCGILGMHTIQDRPVAIAGRVEIRPMMYLALTY"
BASE COUNT          536 a          771 c          780 g          467 t
ORIGIN      
        1 gagccgcgct tcacccagcc gctgatgtat tcggcgatcg aggaccagcc gcccatcagc
       61 gatattttct tcggccatct ggtgaagttc ggcgacatca cgccggacga agtgcgcgct
      121 ttccgcgcca ccttcgagga acggctcaac gacgcgctgc aaaaatcgaa agtggcgacc
      181 aagacgatcg tcccggccct gcgcaaatcg atgtcttgtc cggcattgct cgatccattt
      241 gaaagcgcgg tgccgctgga gaaattgcgc gaaatcggcg cggcgatcac ccgggaaccg
      301 gaaaagatcg agctcaatcc gaagatcaag cgctggctgc agagccgcca cgacatgatc
      361 gagggcaagg cgcccctcga ctggagcacc gccgaggcgc tcgccctggg cagcctgctc
      421 gaaagcggtt atccggtgcg tctttcgggc caggacagcc gccgcggcac gtttacccag
      481 cggcattcgg tgctctacga catgaagacg cgcgagcgct acacgccgct caacaatatc
      541 aagccgggcc aggccaagtt ctgcgtctac aacagccccc tctccgaata tgccgtgctc
      601 gggttcgatt tcggttacac gctcgatttc cccgacatgc ttgtgctctg ggaagcgcag
      661 ttcggcgatt tcgccaacgg cgcgcagatc gtgatcgacc agtatgtcgc ctgttccgag
      721 acgaagtggg gcgagacatc gggcatcacg ctgcttttgc cccacggtta tcacggccag
      781 gggccggaac attcgagcgc tcggctggag cgttatttgc aggcatgcgc cgaggacaac
      841 atccaggtgg ccaacccctc gacgcccgcg agctacttcc atatcctgcg ccgccaggct
      901 ttgcgcaaga tcaagaagcc gctcgtcttg atgacgccga agggcatgtt gcgcgatccg
      961 cgctgcacat cgcccatcga gaactttagc aaaggccgct tcgaggaaat tttgcccgat
     1021 acgatcgcgc cgaaagaggc caagcggatc gttttttgca gcggcaagat ttactttgat
     1081 ctcgtcgatc atctcaaggc ggagaacatc cgggatgccg cgattgtccg cgtcgagcag
     1141 ctttatccgc tgcatgaaaa gaagcttcgg gatgccgtcg cctcctatcc caagctggaa
     1201 aagatcgtct ggtgccagga ggagtcggcc aacatgggcg cgtggaattt catcgagccg
     1261 cggttgcgca aactgtttgg ccgggacatc ctctatgccg gtcgcgatgc ctcggcgagc
     1321 acggccacgg gcgcgactgc cattcacgaa ctcgaacagc gcgaactgat caagcaggcg
     1381 tttactcttt gatttaaatt catcctttta tgcccatcga agtcaaagtc ccctctgtcg
     1441 gcgaatcgat cacgtccggt attctcggcg tctggaacaa accggacggc agttacgtga
     1501 aggcgggcga acccattttt gaagtcgaga ccgacaaggt gacgtccgag gtcgtggccg
     1561 agagttcggg ccaactgaaa catctggcga aagcgggcga cacggtgaag atcggccaga
     1621 tcgtggccag catcgatgaa aaggccgccg cacctgccga agccgctccc gcgaaagtcg
     1681 agccgaagaa ggaaacggcc aacggcacaa cggagaaaac ctcccgcctg gaagcctcgg
     1741 cgccggtcgc ggcttccgcg gcccggggga agaatgcgga cgatctttcg cccgccgttc
     1801 gttatcaggc ggaggaaaag ggcatcgacc cggcttcgat caaggggacg ggcaaggagg
     1861 gacggattct caagggcgac gtgctcgcgg ctgggacttc cgcggacagc agcaaggcgg
     1921 ctgctgttcc cgcaccgcaa cccctaggcg aacggacctc gcgccggaag atgacgccgt
     1981 tgcgccagaa aattgcggcg cgtcttttga gcgcccagca ggaaacggcc cacctgacca
     2041 ctttcaacga ggtcgacctg accaacatca tggcgctgcg gacgaagttc caagaccgct
     2101 ttgtgaagaa gcacgaggtg aaactcggct tcatgtcgtt tttcgtgaag gcggtgctgc
     2161 acgctctgca ggtcgttccg gcggtcaacg cgcggatcga cggcgaggaa gtggtgcaga
     2221 atcatttcta cgacatcggc gtggcgatct cgaccgagaa gggcttgctg gtgccggtcc
     2281 tgcgcgacgc cgacaagctc tcctttgccg gagtggagaa agccatcgcg gcctacgcga
     2341 aaaaggcgcg cgacgggaag attacgctga gcgatctcga aggcggcgtc tttacgatca
     2401 ccaacggcgg cgtcttcggt tcgctgcttt cgacgccgat tctcaatccg ccccaatgcg
     2461 gcattctggg catgcatacg atccaggatc ggccggtggc cattgcgggt cgcgtcgaaa
     2521 tccggccgat gatgtatctc gcgctgactt acga
//
LOCUS       DATMNS010000312         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4199059, whole genome shotgun sequence.
ACCESSION   DATMNS010000312 DATMNS010000000
VERSION     DATMNS010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4199059"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VNW28_10970"
     CDS             <1..186
                     /locus_tag="VNW28_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10495.1"
                     /translation="PPQEIYKVAEEGQKELSKRAHLHVIYYLNGKEKPDEVAMLRGLA
                     SRNGGKFQKVEAKGRKK"
     gene            340..825
                     /locus_tag="VNW28_10975"
     CDS             340..825
                     /locus_tag="VNW28_10975"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXA10496.1"
                     /translation="MHKVNLNEIKEEERKSPKEKFHKFVKDISLALGREKNSLDLAKR
                     HPFDLALVRIPKGKSYCPYHGHGAETELYLVVSGRGKVRDHHGTTEVGAGDSFLFAPG
                     EAHQLSATENEDFVYYVIADNPRGDTCYYPDSGKWVAFQEGGEEIIAKGAEADYYDGE
                     E"
     gene            1057..3174
                     /locus_tag="VNW28_10980"
     CDS             1057..3174
                     /locus_tag="VNW28_10980"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HXA10497.1"
                     /translation="MASRRRLAFLLALLVLCPIAARSQKFEGRDLVTAQLLADTATVV
                     PGQPFTAGLFLKMAPGWHTYWQFPGDAGIPTEIKWNLPPGWKAGPMQWPVPLKLTEPG
                     DIQIYGYHDEVLLMMQLTPPPKISGDSVKLDGTADWLVCARICIPGSASVQLQLPVGG
                     KSTPANQELFSKFRDRLPRPLPPSASAALHWSRQPNEFRLTIADRSLAQASGVDFFPL
                     PVAPTITGHPRREQSADGSVVFVIPLEQAGKDVRSLDGLIVAGDHAFSLGDKPATGGS
                     VADNPVPGAAPDGLLKLLLFGFIGGFILNLMPCVLPVISLKIFGFIQHAGDSRKRIFG
                     NGLAFTAGIFAWFIGLALLMIALKSAGREITWAFQFTNPYFVVAMSAVVLVFALNLFG
                     VFEITLPAGAATGLLGWSAREGYAGSFFQGVFATVLATPCTAPYLGTALGFAFAQSAL
                     IILLMFVAIAAGMSTPYLLLSAQPAWLRFVPRPGPWMVRVKQFMGFLLLATLLFLLWV
                     LGIARGVDATIWVSAFLLALSIACWMQGSFSTPTASLSQRLLVFVLMLVLVLGSGFYF
                     IGQKFAATRTVASSLTNSDWVPFSPERLQSELAQGHSVFLDFTAAWCITCKFNEAAVL
                     ESAAVRSAFERYGIVKMKADWTNADPVITKTLKQFGRVGVPLYVLYPATAPNQPVVLP
                     ELLTQALVLDHLQSAATKVAATK"
     gene            3181..>3387
                     /locus_tag="VNW28_10985"
     CDS             3181..>3387
                     /locus_tag="VNW28_10985"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HXA10498.1"
                     /translation="MKTKLLLLALTTVSASALCAAEAPKVGAPAPAFSLPASNGKTIS
                     LGDFRGKYVVLEWFNPGCPFVQKHY"
BASE COUNT          627 a         1096 c          974 g          690 t
ORIGIN      
        1 ccgccgcagg agatttacaa agtggcggag gagggccaga aggaactatc gaaaagggcg
       61 cacctccatg tcatctacta tttgaacggc aaggaaaaac cggatgaggt tgcgatgttg
      121 cgcgggctgg caagtcgaaa cggcgggaaa ttccagaaag tggaagcgaa ggggcggaag
      181 aagtagcacc agcccggtag acgcttcgct ctgcgaagtg cgggtgcagg cgcggcccgt
      241 tttgaggctt cttcaaattt catccctacc cccgcgcctc ccacagggag gcgactacaa
      301 tttgctcgcc tttggccgcc gctcgcagaa ttttctctca tgcacaaggt gaacctcaac
      361 gagatcaaag aagaggaacg gaaatcgccg aaggaaaagt tccacaaatt tgtgaaagac
      421 atctcgctcg cgctcggccg cgagaaaaat tcgctcgatc tggcgaagcg gcacccgttc
      481 gaccttgcgc tcgtccggat tccgaaaggc aaatcctact gtccctatca cgggcacggc
      541 gcggagacgg aactttatct ggtcgtctcc gggcgaggca aggtgcgcga ccatcatggc
      601 acaacagaag tgggcgcggg cgactctttt ctcttcgcgc caggcgaagc ccatcagctt
      661 tccgcaaccg agaacgaaga tttcgtttac tacgtgatcg cggataatcc gcgcggcgac
      721 acctgttact atcccgacag cgggaagtgg gtggccttcc aggaaggcgg cgaagagatc
      781 atcgcgaagg gcgccgaagc cgactactac gacggggaag aatagcgatt ggacattctt
      841 cgctggtctt ttgtccgggg agcgcacgcg cctcgcgtgt tgtctccggc gcctcgccgg
      901 agacgtcttc tacgggtggc gtttggaatt ggaggccgcg cgttttggtg ccgccgactt
      961 ttcggcgagg cgccgaaaag agcacgcggg gcgcgtgcgc tccccggata agactccggg
     1021 cgttgccgcc cttcggcagc gatggtaaac aatcggatgg cttcccggcg caggctcgca
     1081 tttcttctgg cgctgctcgt gctctgccca atagcggcgc gcagccagaa gttcgaaggc
     1141 cgcgatctcg ttaccgccca gctcctcgcc gatacggcaa cggtagttcc cggccagccg
     1201 ttcaccgccg gtctgtttct gaaaatggcg cccggctggc atacctactg gcaatttccc
     1261 ggcgacgccg gcatcccgac cgagatcaaa tggaacctgc cacccggttg gaaagcaggc
     1321 ccgatgcaat ggcccgtccc gctcaagctg accgaaccgg gcgacattca aatctacggc
     1381 tatcacgacg aagtcctgct catgatgcag ctcacgccac cgccgaaaat ctcgggcgac
     1441 tcggtcaagc ttgacggcac cgccgactgg ctcgtctgcg caagaatttg cattccggga
     1501 agcgccagcg tgcaactcca gttgccggtc ggcggaaaat ccactcccgc caaccaggag
     1561 ctgttctcga aatttcgcga ccgcctcccc cggccactgc cgccgagcgc gagcgcggcc
     1621 ttgcattgga gccgtcagcc taacgaattt cggctgacga tcgcggaccg aagtctcgcg
     1681 caagcgtccg gggttgattt ctttcccctg cccgtggctc cgaccatcac cgggcatccg
     1741 cgtcgtgaac agagcgccga cggttccgtc gtcttcgtca tcccgctgga gcaggcgggt
     1801 aaagatgtgc gctcgctcga cggactgatc gtcgcggggg atcacgcctt ctcgttaggc
     1861 gacaaaccgg ccacgggagg cagcgtagcg gataatcccg tgccgggcgc cgcacctgac
     1921 ggcctgctca agctcctcct cttcggtttc atcggtggct tcatcctcaa cctgatgccg
     1981 tgtgtccttc cggtgatctc actcaagatc ttcgggttca tccaacatgc gggcgacagc
     2041 cggaagcgca ttttcggcaa tggcctcgcc tttaccgccg gcatcttcgc ctggttcatc
     2101 ggactggcgc ttctgatgat cgcgctcaag tcggccggcc gcgaaatcac ctgggctttt
     2161 caattcacca atccctattt cgtcgtcgcg atgagcgcgg tcgtgcttgt tttcgcgctc
     2221 aatcttttcg gcgtcttcga gatcaccctg ccggcggggg ccgccaccgg gctgcttggc
     2281 tggagcgcgc gcgaagggta tgccggctcg tttttccagg gcgttttcgc gaccgtcctc
     2341 gccacgccct gcactgcgcc ctatctcggc accgcgctcg gtttcgcctt tgcgcaatcg
     2401 gcgttgatca tcctgctcat gtttgtcgcc atcgcggcag gcatgagcac tccctacctg
     2461 ctgcttagcg cgcagccggc ctggctccgc tttgtcccgc gccccggtcc ctggatggtg
     2521 cgggtgaagc aattcatggg ctttctcctc ctcgccaccc tgctcttcct gctctgggtg
     2581 ctcggcatcg cgcgcggggt ggacgcgacc atctgggtga gcgcgttcct ccttgcgctc
     2641 agcatcgcct gctggatgca aggatcattc tccacgccca ccgcctcgct ttcccagcgt
     2701 ctcctcgtgt tcgtgctcat gctcgtgctc gtgctcggca gcggctttta tttcattggt
     2761 caaaaattcg ccgccaccag gaccgtcgcc tccagcctaa cgaacagcga ctgggtcccg
     2821 ttcagtcccg agcgcctgca aagcgagctc gcgcagggcc acagcgtctt tctcgatttc
     2881 accgcggcct ggtgcattac ctgcaaattc aacgaagctg cggtcctcga aagcgcggcg
     2941 gtgagaagcg ccttcgagcg ctacggcatc gtgaaaatga aagctgattg gacgaacgct
     3001 gacccggtga ttacaaaaac tttgaaacaa tttgggcgcg tcggtgtccc attatatgtg
     3061 ctctacccgg cgacggcccc gaaccaaccg gtcgtcttgc cggaactgct cacccaggct
     3121 ctggtcctgg accatctcca atccgccgcg accaaagtcg cggccaccaa ataaacaatc
     3181 atgaaaacaa aactgctcct gctcgctctc accacggtct ccgccagcgc gctctgcgcg
     3241 gccgaagcgc ccaaggttgg tgcccccgcg cccgccttct cgctgcctgc ttccaacggc
     3301 aaaaccattt cgttaggcga tttcagaggc aaatacgtcg tccttgaatg gttcaacccg
     3361 ggctgtccct tcgtgcagaa gcattac
//
LOCUS       DATMNS010000313         5399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3868568, whole genome shotgun sequence.
ACCESSION   DATMNS010000313 DATMNS010000000
VERSION     DATMNS010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3868568"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VNW28_10990"
     CDS             complement(<1..429)
                     /locus_tag="VNW28_10990"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HXA10499.1"
                     /translation="MIALALFLATCFLAYANGANDNFKGVASLFGCKAASYRTAIAWA
                     TITTFAGSITAVFLAQALLKKFSGKGLVPDAIVGSAPFLLAVAIGAALTVILATRFGF
                     PISTTHGLMGALIGGGLVAVGGGVNFAALGKGFVLPLLLSP"
     gene            complement(426..1304)
                     /locus_tag="VNW28_10995"
     CDS             complement(426..1304)
                     /locus_tag="VNW28_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HXA10500.1"
                     /translation="MSETIRATRPEPPPHVSAEIKVAHAPLPKWVIGEFFGTFLLVFF
                     GCGSVCAAVATGAQVGIFQVAIVWGLGIATAIYLTGSLSSAHLNPAVTIASAAWSRFP
                     FRLVVPYIIVQMLGAFAAAAVLFSIFNGAIAGYEAKHGITRGAEGSEATAMVFGEYFP
                     NPGGKALTEEARATMTPGHAFTAELVGTAILVLVIFCSTDERNAARPQILTAATIGLT
                     VTLVISLIGPLTMAGLNPARDLAPRIFSSLAGWKSVPFTVNGSGWLTVYIIAPIVGGL
                     LGGGIYRVFFRAAYRV"
     gene            complement(1320..2474)
                     /locus_tag="VNW28_11000"
     CDS             complement(1320..2474)
                     /locus_tag="VNW28_11000"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXA10501.1"
                     /translation="MSTINAETAVRERYAAGAKAAEAKLCCPVEYNPEHLKVIPQEVI
                     ERDYGCGDPSRYLRPGETVLDLGSGTGKICFIAAQVVGPKGKVIGIDMTDEMLNVART
                     NTPIVAERIGYNNVEFRKGRIQDLALDLEKLDAELQARPITDAASFLRADELAQDLRV
                     MHPLVASDSVDVVVSNCVLNLVEPKSKRSLFEEIFRVLKKGGRAIICDIVSDEDVPEH
                     LQNDPELWSGCISGALTEEGFLQAFGESGFYGIQILKRDEQPWQTVEGIEFRSVTIEA
                     FKGKQGSCFERNQAVIYRGPFREVLDDDNHRMERGVRYAVCDKTYNLYHKAPYREFFE
                     FVEPLVDIPLAEAQPFDCSHTARRHPKETKGQDYKVTTEASSCCDGGSCG"
     gene            3350..4699
                     /locus_tag="VNW28_11005"
     CDS             3350..4699
                     /locus_tag="VNW28_11005"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXA10502.1"
                     /translation="MKARSIFPAVVLLAAGCATPDASLDRVQSTVAARAGKRVHWMRG
                     GPEDAEIEQGVQAILRRPLTAERAVQVALLNNRELQARFEEIGIAQADVIQAGLVSNP
                     SFAASFRFPDRSPSGTNIEYSIAQNFLDLLVMPLRKRVAAAQVAEIETRTADEVLKLA
                     SEVKIAFYTAQARQQLLDRLRVIAATNETAPEFTKRLHDAGNTSDLELANQEGSYEQS
                     RIDVAQTELQVRRDRERLNRLLGLWGGNTNWAMGDHLPEVPDRESSLQNLEARAVAQR
                     LDLQAARMRLDVIGQSLALRAKTRYLPAEIKVGVDTEEAPDSQRVTGPTLDLELPIFN
                     QGQGEIAKLTAQYRQAERELEAMAINIRSEVREARDQLVAARDLTSYIGKKLLPTQKE
                     ALNLTLQQYNYMLKGAYDLLLAKQNEVAAERSYIEAWRDYWIARTELERAVGGKLHQ"
     gene            complement(4867..5250)
                     /locus_tag="VNW28_11010"
     CDS             complement(4867..5250)
                     /locus_tag="VNW28_11010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXA10503.1"
                     /translation="MGAMEKKTKPLNDRPYDLEERTFLFAQEARNLVKTLPRTIENRE
                     DIRQLVRSSGSVGANYIEANESVSKKDFLLRIKTCRKEAKESAYWLRLLDCERSAALQ
                     AECGRLTTEARELMNIFGAILRKSE"
BASE COUNT         1116 a         1664 c         1590 g         1029 t
ORIGIN      
        1 cggactgagg agcaacggca gaacgaaacc tttgccgagc gcggcgaaat tgaccccgcc
       61 gccgaccgcc accagtccgc cgccgatgag cgctcccatg aggccatgag tggtggaaat
      121 cggaaatcca aaacgggtcg caaggatgac ggtaagggcg gcaccgatcg cgacggcgag
      181 caagaatggt gcggagccga cgatcgcgtc gggcacgagt cctttgccgg aaaacttttt
      241 caacaacgcc tgcgcgagaa agaccgcggt gatcgagccg gcgaaagtcg tgatcgtggc
      301 ccaggcgatg gcggtgcgat aactggcagc tttgcaacca aagagcgaag ccacgccttt
      361 gaaattatcg ttcgcgccgt tggcgtaggc gagaaagcac gtcgcgagga agagagcgag
      421 cgcaatcata cgcgataggc cgcgcgaaag aagacgcggt agattccgcc gcctaacaat
      481 cccccgacaa tcggcgcgat aatgtaaacc gtcagccacc cggagccgtt gacggtaaac
      541 ggcacactct tccagccggc gagagaggag aaaatccgcg gcgcgagatc gcgcgccggg
      601 ttgagcccgg ccatcgtgag cgggccgatc agagagatga ccaaggtgac cgttaaacca
      661 atcgtggccg cggtcaggat ctgtgggcgc gcggcattac gctcatcggt tgaacagaaa
      721 atgaccagca caagaattgc cgtcccgacc agttccgcag tgaaagcgtg acccggcgtc
      781 atcgtggcgc gcgcttcttc ggtgagcgct ttgccgccgg ggttgggaaa atattcgccg
      841 aagaccatcg cggtcgcttc gctcccttcc gcaccccgcg tgatgccgtg cttcgcctcg
      901 tagccggcga tcgcgccgtt gaagatcgag aagaggaccg cggcggccgc gaacgccccg
      961 agcatctgca cgatgatgta gggaacaacc agcctgaacg ggaaacgcga ccaggcggcg
     1021 gaggcgattg tgaccgcggg attcaagtgc gcgctgctca gcgaaccggt caggtaaatc
     1081 gcggtcgcaa tcccgaggcc ccagacgatc gcgacctgaa aaatcccaac ctgcgcgccg
     1141 gttgcgaccg cggcgcagac actcccgcag ccaaaaaaca cgaggagaaa cgtgccgaag
     1201 aattcgccga tcacccattt cggcagcgga gcgtgcgcga ccttgatttc cgcgctgaca
     1261 tgcggcggtg gctccgggcg ggtggcgcgg atggtttcgc tcatggagca acgcgatccc
     1321 tagccgcagc ttccgccatc gcagcaactg ctcgcttccg tcgtgacctt gtaatcctgg
     1381 cctttggttt ccttcggatg ccgccgggcg gtatgcgaac aatcaaacgg ctgcgcttcg
     1441 gctaatggaa tgtcgaccag cggctcgacg aactcgaaga actcccgata gggtgccttg
     1501 tgatagaggt tgtaggtctt gtcgcaaacc gcgtagcgca ctccgcgctc catccggtgg
     1561 ttgtcatcgt cgagcacttc gcggaacggc ccgcgataga tcacggcctg gttgcgctcg
     1621 aagcaggaac cttgcttccc cttgaacgcc tcgatcgtga cggagcgaaa ttcgattccc
     1681 tccaccgtct gccacggctg ctcgtcgcgt ttcaggatct ggattccata aaaaccggat
     1741 tcgccgaagg cctgcagaaa accttcctcc gtcaatgcgc ccgaaatgca gccgctccac
     1801 aattcgggat cgttctgcaa atgctccggc acgtcttcgt ccgagacaat gtcgcagatg
     1861 atggcgcggc cgcctttttt caggacccgg aaaatttcct cgaacaagct cctcttcgac
     1921 ttcggctcga ccaggttgag gacgcaattg gaaaccacga cgtcgaccga atcgctcgcg
     1981 acaagcggat gcatgacccg caaatcctgg gcaagctcat ccgcgcgcag gaaagacgcg
     2041 gcgtccgtga tcggtcgcgc ctggagttcg gcatccagct tttcaagatc gagcgcgagg
     2101 tcctggatgc ggcctttgcg gaactcgacg ttgttgtatc caatccgctc cgccacgatc
     2161 ggcgtgttgg tgcgcgcgac attcagcatc tcgtccgtca tgtcgatgcc gatcactttt
     2221 cccttcggcc cgacgacctg cgcggcgatg aaacagattt tcccggtgcc actgccgagg
     2281 tcgagcacgg tctcgcccgg gcggagatac ctgctcggat cgccgcagcc gtaatcgcgc
     2341 tcgatcacct cctgcggaat gaccttaagg tgttccggat tgtattcgac cgggcaacaa
     2401 agctttgctt cggcggcttt cgctccggcg gcgtaacgtt ctctgacggc ggtttcggcg
     2461 ttgatcgtgc tcatcgtgaa aggggtaggt tgaagttgga ataagagaac tgtaaggccg
     2521 taaaattcca agcaagggaa tctctcagcc tacgtgttac gcacgggata cgggaaagga
     2581 tgcccagcgg caagctgaaa cgcacaaacg gactttcagg gcggcaagcg gttgacgcga
     2641 atggcttttc acgccagaga gatatcgtgt tgcgcgcgcg ccgagtgacg ccccttcgtt
     2701 gggcaacggt gacgctggct gcgatttcgt ggctcgcgat ctcgaatcat tgcgctctgg
     2761 gacgggccgc gagcgagagc catgaaacag aagcgctcgc ggaacacgat tgctgcgcgg
     2821 gcgagctgcc ggccaaaccg aagcccgccg gggacccaaa cgcgccctgt tgcaaaactc
     2881 tacgcgcgac gacggcgctg gcgaagtcgt tccgggccgg tgaggtaagg ctggccggag
     2941 ccccggttga atttcccccg gcgctcctgg ataagccgcg cgcttttcat ctcgcgcgtc
     3001 gctccctcga taccgggccg cccgggacaa gctcgttcgc tgaatcggtc ctgcagcgaa
     3061 gtcttctcgc gcacgctccg ccgttcgtcg cctgacgatc tccgatcact cgcctggcga
     3121 gccgagccga gatcaccgcc ggattgcgcc cgtcttacgt cgcgggcccg cctttgcttt
     3181 ccgcgttccg aaaccgtttc accttcatgt ttatttattc tgaatttcaa aatcactccg
     3241 cgctgccgtt ccagtcgagg ccgctcgcga attcgcgctc tcttccttcc cccgcgatcc
     3301 ggggaatgac tttggccagg aaaattcccg cgccggaagg agccaggcag tgaaggcgcg
     3361 atccattttc cccgctgtgg tgcttctcgc ggccggctgt gccacaccgg atgcttccct
     3421 cgatcgcgtg cagtcgacgg tggcggcgcg agccggcaag cgcgtgcatt ggatgcgcgg
     3481 cggccccgaa gacgcggaaa tcgagcaggg cgtgcaagct attctccgtc gcccattgac
     3541 cgccgagcgc gcggtccagg tcgctctcct gaataatcgc gagctgcagg cgcgcttcga
     3601 agaaatcggc atcgcgcagg ccgacgtgat tcaagccggc ctggtgagca acccgagttt
     3661 cgccgccagc tttcgatttc ccgatcgctc accctcgggg acgaacatcg agtattcgat
     3721 cgcgcaaaat tttcttgatc tgctggtcat gccgttgcgc aagcgcgtcg ccgctgccca
     3781 ggtcgcagag atcgaaacgc ggaccgcgga tgaagtgctg aagcttgcgt cggaggtcaa
     3841 gatcgcgttc tacaccgccc aggcgcggca gcagttgctt gatcgcctgc gggtcatcgc
     3901 agcgacgaac gagaccgcgc cggagttcac caaacgtctg cacgacgctg ggaacacaag
     3961 cgacctggag ctggccaacc aggagggcag ctacgagcaa tcccggatcg atgtggcgca
     4021 gaccgagtta caagttcggc gcgaccgcga gcggctcaat cgcctcctcg ggctctgggg
     4081 aggaaacacc aattgggcga tgggagatca cctgccggag gtgcccgacc gcgaaagctc
     4141 cctccagaat ctggaagcgc gcgctgtcgc gcaaagactc gacctgcaag ccgcgcgcat
     4201 gcgtctcgac gttatcggtc aatctctcgc cctgcgcgcc aaaacgcgct acctgcccgc
     4261 ggaaatcaag gtcggcgtcg ataccgagga agcgccggat agccagcggg tgaccggacc
     4321 aacacttgac ctggagttac cgatctttaa ccaaggacag ggcgagatag caaaactgac
     4381 cgcgcaatat cgccaggccg agcgcgagct ggaggcgatg gcgatcaaca tccgctccga
     4441 agtacgtgag gcgcgcgatc aactggtcgc agcccgcgat ctcacttcct acatcggaaa
     4501 gaaactcctc ccgacccaga aagaggcact caacctcact ttgcagcaat acaactacat
     4561 gttgaaaggc gcttacgacc tgttgctcgc caaacaaaac gaagtcgccg ccgagcgcag
     4621 ttacattgaa gcgtggcgcg actactggat cgcgaggacc gagctggaaa gggccgttgg
     4681 agggaaactg catcaatgaa atccgaaatc cgaatatcga aatccgaaac aatcagcaaa
     4741 atggttgaaa ttaaggaaag gcaaaaatcg gcgcggtttc ctcgtttgct cgattcccaa
     4801 gtttcaactt ccgtttcctt cggatttcga tccgcctcag gcggaccgaa tttcgggctt
     4861 cttcccttat tcgctcttac gcagaatcgc gccgaagatg ttcatcaatt cccgggcttc
     4921 agtggtcagg cggccgcact ccgcttggag cgccgcactc ctttcgcagt cgagcaaccg
     4981 cagccagtag gcggattcct tcgcctcctt ccggcaggtc ttgatccgca gcaggaaatc
     5041 ctttttgctc accgactcat tcgcctcaat gtaattggcg cccaccgagc cggaggagcg
     5101 aacaagctgc cttatatctt cgcgattctc tattgtccgc ggaagcgttt ttacgagatt
     5161 tcgcgcctcc tgagcgaaca gaaacgtccg ctcctccaaa tcgtaaggtc ggtcattcaa
     5221 cggttttgtt tttttctcca ttgcgcccat tcaaattcca tttgtttcgg atttcgacat
     5281 tcgaatttcg gattttcctt cttatgatta cccgacgtaa atttttctca ggcaccgcgg
     5341 cgcttcttgg cgcgtcggcc ctcgcgcaac gcatcgccgg cggaacgcag ttactcgcc
//
LOCUS       DATMNS010000314        10447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3875896, whole genome shotgun sequence.
ACCESSION   DATMNS010000314 DATMNS010000000
VERSION     DATMNS010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10447
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3875896"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2034)
                     /locus_tag="VNW28_11015"
     CDS             complement(<1..2034)
                     /locus_tag="VNW28_11015"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXA10504.1"
                     /translation="MQGLEINLLVPDLWQQEAVRALQAGRDVVVHAPTGAGKTYIFEL
                     LYPSLKGQAVFTVPTRALANDKLAEWRARGWDVGITTGDLALNLEAKVVVATLETQRT
                     RFLRRDGPRLLVVDEYQLIADPVRGVHYELSLALAPPGTQLLLLSGSVNNPQDVVEWL
                     RRIGRDAVLVRHDQRPVPQHEVDLRSLPEVGFLQVREFWPRMIAKALHGGLAPILVFA
                     PRRNATEEMAQAIASAVPVRDPLPLSPQQEALAGKRLTKLLRSRVAYHHSGLSYAVRA
                     GLIEPLAKAGQLNVVVATMGLAAGINFSMRSVLITDTRYFSGNFERQVQPDELLQMFG
                     RAGRRGLDDTGFVLFTPDQPRLSFARPRQLKRATQVDWPSLISVMALAGGIDPGGLGS
                     RETAGVAAAGYKAAVEVSRSLFSAQRVPLGVEHSLATGPKPCGLWVDAERARFVRRPI
                     VEIRNSREAWEPRGEKVDTSLGEALLRENDRWIPALRLPKTLDRFPLGNLCRLPEKKI
                     YGRELPLATIGPEQADLQLVKPLRKTWQVKSLPEGELRRRVHESLPEWSGGGVVDEFV
                     RRGNLLSVRIDFSRVPLKAWRDSSGQALLDPPERAALPPVCRGCSELEHDQTVEIVAS
                     PAFAWRRLGLVEPDGKPTRRGVLFGFFQSGEGLAIAAALEDETYPIDELIFDLA"
     gene            complement(2278..2925)
                     /locus_tag="VNW28_11020"
     CDS             complement(2278..2925)
                     /locus_tag="VNW28_11020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10505.1"
                     /translation="MRILPVLLAVVLALSLGACGTSRNLPAYERPLARTDFQQVRTTA
                     YTHTEADHEAYGNHNAMGGILHAAAPPFVPRAIPVASADNRSRRDGYQSIAYVSPSQS
                     FLGDRLSDQIYGSAAADWSRWPAGTIFRVVSTGQLYRVEDYGWALAGRNTIDLYMATP
                     RDMNNWGARPELIQIVQWGDPQESLRRLAPHTKYRHIRRMVLELEGKQRAAANLE"
     gene            3098..4282
                     /gene="kbl"
                     /locus_tag="VNW28_11025"
     CDS             3098..4282
                     /gene="kbl"
                     /locus_tag="VNW28_11025"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HXA10506.1"
                     /translation="MLAKFADHVSQTLEEIKAQGLFKTERIITGPQNAEIPIADGRRV
                     INLCANNYLGLADHPALIAAAKAALDSHGFGMASVRFICGTQDIHKELEAALTKFLGT
                     EDTILYPSCFDANGGLFETLLGAEDAVISDELNHASIIDGVRLCKAQRYRYKNCDMAD
                     LETQLQAAKEARFRLVATDGVFSMDGTIANLKGVCDLAEKYDAMVMVDDAHAAGFIGK
                     TGRGTPEHCGVQGRVDVITGTLGKALGGASGGYTSGRKEIVALLRQRSRPYLFSNTVA
                     PPIVAASLQVLTMLTASTDLRDKLAENTKYFRAALTERGLTIKPGTHPIVPIMLGDAT
                     LSQKFATRMLEKGVYVVGFFYPVVPQGTARVRTQVSAAHSRVELEQAVKAFAETNTEL
                     AG"
     gene            complement(4361..5551)
                     /locus_tag="VNW28_11030"
     CDS             complement(4361..5551)
                     /locus_tag="VNW28_11030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10507.1"
                     /translation="MDPTLRARFNAEFSPARYEAFLNCVQHTERWPADFRISETPVFL
                     TREFTDEVVGAANEIVVRTRTTEFARHAASAIPPGLEVPNETAHPNFFVVDFGICEEG
                     GRLTPRLIELQAFPSLYGFQFLLLGCIRQNFPVIPRNWTSAFSGLKDESYLSLLREVI
                     IGPAHPENVILLEIEPEKQKTRIDFACSESLIGVRPVCLTKIRKRGRQLFYERDGREV
                     RIERIYNRVIFDELLRRPDVTPGFHFQDELDVVWAGHPNWYFRVSKHSLPFLKTAHTS
                     PAYFADQFPVDESLEDYVLKPLYSFAGLGVDMEPTREKLAALEAPQNWILQKKVRYAE
                     FLQTIDGRRSKAEIRMMFLWPDGGEPILANNLVRMSQGKMMGVDFNKNKTWVGASIAL
                     HQVG"
     gene            5713..7110
                     /locus_tag="VNW28_11035"
     CDS             5713..7110
                     /locus_tag="VNW28_11035"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HXA10508.1"
                     /translation="MTAAAQPEDAAPLLGEDHRPDFLGHPRGLWYLAFTEAWERFSFY
                     GMQALLVLYMVKYLLLPGRIEHVVAFDAFRRLPLYSGLQGQALASAIFGTYAASVYLT
                     PIFGGFLADRILGRRRTVLLGALTMAAGHFLMAFEAAFLFALLCLVLGSGMFKGNIAS
                     QVGSLYKPEDLRRADAFQIFYLGINAGVIASPLIVGTLGEVYGWHYGFAAAGVGMLLA
                     IGIYLSGQKYLQGEEIDARAAVAAKTPRAKLTGRDWRAVIALLLLIPVLAVAIVPNNQ
                     IFNAYLVWGDQQFDLVWFGKKLPTTWLVTLDAIVSVSFLALVALFYRWYGKRWREPDE
                     TTKLIIGSGFSIGGTLCLFTAAATQQAGHKIGLFWPVAFHFVNSIAFAHLLPISLALF
                     AKYAPKAINATVIGLYYLAFFCANSLVGWVGGFYQTMPTTTFWLLHTGFAAGSGLCFV
                     IFKFVASHHLTVEPG"
     gene            7234..9786
                     /locus_tag="VNW28_11040"
     CDS             7234..9786
                     /locus_tag="VNW28_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXA10509.1"
                     /translation="MLHDFRFAFRQLVKARAFTAAAVIVLALGIGANTAIFSLMNTLL
                     FQRPHYTKADEIVQVFSQDKKNPKTFRGFSYPTYLDIRDQNSVFSGVMAHNLAMVGVG
                     DKGNTRRAFTDVVSANYFSVMGVMPIQGRAFRAEEETPDTNSAVAIVSYNYWKKEGLN
                     PAMLGSTITIDSRPFTVIGILPQGFTGTTQIFAPEIWLPLGVYDQVANDFETENKDKL
                     DDRGGRQLMIVGRLKPGVTAAAAAPALKTLAASLEQAYPVEQKDQTFTLETLSRFAIS
                     TSPTSGNNIKRLAPLLFGMSGVVLLVACLNLANMLLARGAARRKEIAIRLALGASRGR
                     IVRQLLVEGFVLALAGGGFGLLLGLWSSDLLVASLGKKMPIDIVWQGGLSAPVLAATF
                     AFCLLGTLAFALGPALKLSKAAVIGDLKEQAGEDTVVRRGKFWPRNPLVVVQIAFSLA
                     LLTAAALFIRGAGKAAAVETGLQTSSNYLLEVDASLATYDQARSLNLYRTLEERLAAL
                     PGVERASISSTVPFGMVSLDKSVQRGGANPAPDAKPATAAEGLAFTANWNSVGADYFK
                     TVGLPLLRGRAFNISEAMHQTSPKVAIIDEVLAKKLWPDGDALGQRIQFAEKGPTTAK
                     GGGDGTRAQIEVVGIVPYARNDLFEKNPGGMLYLPFAGGFQSNVFFHIKFAPNAGRDV
                     GTTTDLIRRTVREVDAAVPVLTLKTFAQHLEGNLELWIVRAGAALFSTFGALALVLAT
                     VGVYGVKAYSVARRTREIGIRMALGAQRETVQWMILREGTVMLASGVLLGLLLAAGTG
                     KLISGMLYNVGALDPLAFTIAPIVLAAAALIATWLPARRATRISPMAALRTE"
     gene            complement(9983..10309)
                     /locus_tag="VNW28_11045"
     CDS             complement(9983..10309)
                     /locus_tag="VNW28_11045"
                     /inference="COORDINATES: protein motif:HMM:NF025479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HXA10510.1"
                     /translation="MVIYSVTITIDAGIESEWLDWMKRIHVPDVVRTGCFSECRVYKV
                     IDSAVADPSYVMQYHCRSLEEYHRYRDNFAPALQKEHSEKFAGRFRCARQLLKEVADA
                     TLAQEG"
BASE COUNT         1949 a         3301 c         3180 g         2017 t
ORIGIN      
        1 cgcgaggtcg aaaatcagct cgtcgatcgg ataagtttcg tcctccagcg cggcggcgat
       61 agcgagcccc tcgccgctct ggaaaaagcc aaagagaacg cccctccgcg ttggtttgcc
      121 atccggctcg accaagccaa gtcgccgcca ggcaaaagcg ggcgaagcga caatctcgac
      181 cgtttggtcg tgctccagct cggagcaacc gcggcaaacg ggcgggagcg cggcgcgctc
      241 aggcggatcg aggagagcct ggcctgacga atctcgccag gccttgagcg gcaccctgga
      301 aaagtcgatt cggacggaaa gaagattccc tcgcctaacg aattcgtcca ccaccccgcc
      361 gccactccat tctgggagcg attcgtggac ccgtctccgc aattcgcctt ccgggagcga
      421 tttcacctgc cacgtcttgc gcagcggctt gaccagttgc aaatcggcct gctccgggcc
      481 gatggtcgcg agcggcagtt cgcgcccgta aatcttcttc tccggcagcc ggcagagatt
      541 gccgagcgga aaccggtcga gcgttttcgg caggcgcaag gccggaatcc agcggtcgtt
      601 ttcccggagg agtgcttcgc ctaacgaagt gtccactttc tccccgcgcg gctcccatgc
      661 ttcgcggctg ttccggattt cgacgatcgg acgcctgacg aagcgggcgc gttccgcgtc
      721 cacccagagg ccgcagggct tcggtccggt ggccagcgaa tgctcgaccc cgagcggcac
      781 ccgttgcgcg ctgaaaagcg agcggctcac ttcgaccgca gccttgtagc cggcggcggc
      841 gacgccggct gtctcacgcg atccaagacc accggggtca atgcccccgg ctagagccat
      901 cacgctgatc aggctcggcc agtcgacctg cgtcgcgcgc ttcaactgcc gcggccgggc
      961 gaagctgagc cgcggctgat cgggcgtgaa aaggacgaag ccggtgtcgt ccagtccgcg
     1021 gcgaccggcc cggccaaaca tttgcaggag ctcatccggc tggacctggc gctcgaaatt
     1081 tcccgagaaa tagcgcgtgt ccgtgatgag aaccgagcgc atggaaaaat tgattccggc
     1141 ggcgagaccc atagtggcaa cgacgacgtt gagctggccc gccttggcga gcggctcgat
     1201 cagcccggcg cgcactgcgt agctcaggcc gctgtgatga taggccacgc ggctgcgcaa
     1261 taatttcgtc aggcgctttc ccgcgagcgc ttcctgctgc ggcgagagcg gcaacggatc
     1321 gcgcaccggc acggcactgg caatggcctg cgccatctcc tcggtcgcat tgcgtcgcgg
     1381 ggcgaaaacg agaatgggcg cgagcccccc gtgcagcgcc ttcgcgatca tccgcggcca
     1441 gaattcgcgc acctggagaa agccgacctc cggcaggctg cgcaggtcga cctcgtgttg
     1501 cgggaccgga cgctggtcgt gcctaacgag gacagcgtca cgcccgatcc gacgcagcca
     1561 ttcgaccacg tcctgtggat tgttgacgct cccgctaagc aaaaggagct gcgtccccgg
     1621 cggcgcgagc gcgagcgaga gttcgtaatg cacgccgcgt accgggtcgg cgattagttg
     1681 atattcatcc accacgagga ggcgcggacc atcgcggcgc aggaagcgcg ttcgctgggt
     1741 ttcgagcgtg gcgacgacga ccttcgcctc gaggttcagc gcgagatcgc cggtcgtaat
     1801 gccgacatcc cagcctcgcg cccgccattc cgccagcttg tcgttggcca gcgcgcgagt
     1861 gggcacggtg aagacggcct gtcctttgag cgacgggtag agcagctcga aaatgtaggt
     1921 cttgcccgcg ccggtaggcg cgtgcacgac gacgtccctg cccgcctgca aggctcgcac
     1981 cgcctcctgc tgccagagat cgggaaccag caagttgatc tccagccctt gcatccttct
     2041 acaaagatgg acggctgaga ggcacgggca aattgccggg caatttttgt ggggggaatc
     2101 tgtctcgggg tagcgcatgc gtcccgcgtg ctggcgacgg tgtttcaccg tcgcgaactt
     2161 tcccaaacgc agcctacccc agaacgatca cgcgctcaga aaagttcgcc gcgctggaac
     2221 agcgcaacca gcacgcggga cgcctgcgct acccggaggt gagaacggcg gcgctagtca
     2281 ctccaaattg gccgccgccc gctgcttgcc ttcgagctcc agcaccatcc ggcggatgtg
     2341 ccggtacttc gtgtgcggcg cgaggcggcg caggctttcc tgcggatcgc cccattgcac
     2401 gatctgaatc agttccggcc gcgcgcccca gttgttcatg tcgcgcggcg tcgccatata
     2461 aaggtcaatc gtgttccggc ccgcgagcgc ccagccgtaa tcttccaccc ggtaaagttg
     2521 accggttgaa accacgcgaa aaatcgtgcc cgccggccag cgcgaccaat cggctgcggc
     2581 gcttccatag atctggtcgg acaaccgatc gccgagaaaa gattgcgacg gcgagacgta
     2641 agcgatcgat tgatagccat cgcgccggga gcgattgtcc gccgacgcga ccgggatggc
     2701 ccgcggcacg aaaggcggcg cggcggcgtg caggattcca cccatggcgt tatggtttcc
     2761 gtacgcttcg tggtcagctt cggtgtgcgt gtaagccgtc gtccgcactt gctggaaatc
     2821 ggtgcgggcg agcggccgct cataagccgg cagattgcgc gaggtgccgc atgcgcctaa
     2881 cgacagcgcc aacacgaccg cgaggaggac gggaaggatt ctcattccaa cggagccgga
     2941 aagttattaa cagttattca caagctcgac aagggcgttt tcggtctggc cgggacgggg
     3001 ctcacgtggc gccatctaag cagggtcgac agttcccggc gcttcgcaca gcgcagcgtc
     3061 tacattgcat cgccccggct ttgctcgaaa ttgcgcgatg ctcgcgaaat ttgccgacca
     3121 cgtctcgcaa acgctcgaag aaatcaaagc gcagggactt ttcaaaaccg agcgcatcat
     3181 taccggcccg cagaatgcgg agatcccgat cgcggacggc cggcgggtga tcaatctttg
     3241 cgcgaacaac tatctcggcc tggccgatca tcccgcgctc atcgccgcag cgaaagccgc
     3301 gctcgattct cacgggtttg ggatggcgag cgtgcgcttc atctgcggca cccaggacat
     3361 tcacaaggaa ttggaagctg ctctcacgaa attcctcgga acggaggaca cgattcttta
     3421 tccttcctgt ttcgacgcca acggcggctt gttcgagaca ttgttaggcg cggaagacgc
     3481 ggtcatctcc gacgaactga atcacgccag catcatcgac ggcgtgcgcc tctgcaaagc
     3541 gcagcgctat cgctacaaga attgcgacat ggccgatctc gagacgcagt tgcaggccgc
     3601 gaaagaagcg cgcttccgcc tcgtcgcgac cgacggcgtt ttctcgatgg acggcacgat
     3661 cgcgaacctg aaaggcgttt gcgacctggc ggaaaaatac gacgcgatgg tgatggtgga
     3721 tgacgcgcat gccgccggct tcatcggcaa aaccgggcgc ggcacgccgg aacattgcgg
     3781 agtgcagggg cgggtcgacg tgattacggg gacgctgggc aaagcgctcg gcggcgcgag
     3841 cggcggctac acgagcggac gcaaagagat tgtcgcgctc ctccggcagc gttcgcggcc
     3901 ttatcttttc tccaacaccg ttgcgccgcc gatcgtcgcc gcgtccttgc aggtgctcac
     3961 catgctgacc gcctccacgg acctgcgcga caaactcgcc gagaacacga aatatttccg
     4021 cgccgccctg acggagcgcg gcctaacgat aaaacccggc acacatccga ttgtcccgat
     4081 catgctgggc gacgccacgc tttcgcaaaa gttcgcgacg cggatgctgg agaaaggcgt
     4141 ttatgtggtc ggcttcttct atccggttgt gccgcaaggc accgcgcgcg tccgaaccca
     4201 ggtgagcgcg gcacattcgc gcgtcgagtt ggagcaggcc gtgaaagcct tcgcagaaac
     4261 gaacacggaa ttggccgggt aacgggcacc gcgccgacct ggcgaccgtg ttccaccgtg
     4321 gttgccagca cgcaagacgt gtgcgccacc cggcggagtg ctatcccacc tggtgcagcg
     4381 cgatgctggc gccgacccag gtcttgttct tattgaagtc gactcccatc atcttgccct
     4441 ggctcatccg gacgaggttg ttcgcgagga tcggctcgcc cccgtcgggc cagagaaaca
     4501 tcatccgtat ctccgccttc gagcgtcggc catcgatggt ttgaagaaat tccgcgtacc
     4561 gcaccttctt ttgcaggatc cagttctgcg gcgcttcgag cgcggccagc ttttcgcgcg
     4621 tcggttccat gtcgaccccg agccccgcga aggagtagag cggcttgagc acgtaatctt
     4681 cgagggattc gtcaactgga aattgatcgg caaaatatgc cggcgaagtg tgcgcggtct
     4741 tgaggaaggg gagcgaatgt ttgctcaccc gaaaatacca gttcgggtgc ccggcccaga
     4801 cgacatcgag ttcgtcttga aagtggaaac ccggcgtcac atccggccgg cgcagtagct
     4861 cgtcgaaaat cacccggttg tagatccgct caatccgcac ttcgcggccg tcgcgctcgt
     4921 aaaacaactg gcggccgcgt tttcgaatct tcgtcaggca gaccggccga acaccgatca
     4981 ggctttcgct acaggcgaaa tcgatccggg ttttttgctt ctccggctcg atctcgagca
     5041 ggatcacgtt ctccggatgg gccggaccaa tgatgacctc gcggagcaga gagagatagc
     5101 tttcgtcctt caatccgctg aaggccgagg tccagttccg cgggatgact gggaagttct
     5161 ggcggatgca accgaggaga aggaattgaa aaccgtagag actggggaac gcctgcaact
     5221 cgatcaggcg cggggtgagc cgtccgcctt cctcgcaaat cccaaagtcc acgacgaaaa
     5281 agttggggtg ggcagtttcg ttaggcactt ccagtcccgg cgggatcgcg ctggcggcgt
     5341 gccgcgcaaa ttccgtggtg cgcgtccgca ccacgatttc attcgccgca ccgaccactt
     5401 cgtccgtgaa ctcccgtgtc agaaaaaccg gcgtctcgga aatccgaaaa tccgccggcc
     5461 agcgttccgt gtgctggaca caattgagaa acgcttcgta cctggccggt gaaaattcgg
     5521 cattgaaccg ggcgcgcaga gtcggatcca tcggccgtga gcatggcaca acgagccgct
     5581 tacgacaaag cggtcgccga cggctgccac agttccttgc gctcgaaaaa ccgattgaaa
     5641 aacgtgcaac ccgcatcgcc ggggtggctg gcctttacat gtctggtccg ttcccgctaa
     5701 tctccggccc gcatgaccgc tgccgctcaa cccgaagatg ccgcgccgct tctgggcgaa
     5761 gaccaccggc ctgatttcct cggccacccg cgcgggctct ggtacctggc cttcaccgag
     5821 gcgtgggaac gcttttcctt ctacggcatg caggcgctgc tcgtccttta catggtcaag
     5881 tatctcctgc tgccggggcg catcgaacat gtcgtcgcgt tcgacgcctt ccgccggctc
     5941 cctctttata gcggactcca gggccaggcg ctcgcctccg ctatcttcgg tacctacgcc
     6001 gccagtgttt atctcacgcc gatcttcggc ggctttcttg ccgatcgcat tctcggccgg
     6061 cgccgcaccg tgctgcttgg cgccctaacg atggcggccg gccattttct catggccttc
     6121 gaggccgcgt ttctcttcgc gcttctctgc ctggtcctcg ggagcggcat gtttaagggt
     6181 aatatcgcca gccaggtcgg ctcactttat aaaccggaag acctccgccg cgccgacgcc
     6241 ttccagattt tctacctcgg catcaacgcc ggcgtcatcg cctcgccgct catcgtcggc
     6301 acgctcggcg aagtttacgg ctggcactac ggtttcgctg ccgccggcgt cggcatgctc
     6361 ctcgccatcg gcatttacct ttccggccag aagtatttgc agggcgaaga aatcgacgcc
     6421 cgtgctgccg tcgccgcgaa gactccgcgc gcgaaactca ccgggcgcga ctggcgcgcc
     6481 gtcatcgcgc tccttctcct cattccagtg ctcgcggtcg cgatcgtgcc gaacaaccag
     6541 attttcaacg cctacctcgt ctggggcgat caacagttcg atctcgtctg gttcggtaaa
     6601 aaactgccga ccacctggct tgttaccctc gatgcgatcg tcagcgtcag cttcctcgcg
     6661 ctcgtcgcgc ttttctatcg ttggtatggg aagcgttggc gcgaacccga cgagaccacc
     6721 aagctcatca tcggctccgg cttctcgatc ggcggaacac tctgcctctt cacggccgcc
     6781 gccacccaac aggccggtca caagatcgga cttttctggc cggtcgcctt ccacttcgtc
     6841 aacagcatcg cgttcgcgca tctcctcccg atcagcctcg cgctcttcgc caagtacgcg
     6901 ccgaaagcga tcaacgccac cgtcatcggc ctctattacc tggccttctt ctgtgccaat
     6961 agcctcgtcg gctgggtcgg cggcttctac cagacgatgc cgaccaccac cttctggctc
     7021 ctgcacaccg gtttcgccgc cgggtcaggg ctgtgtttcg tgattttcaa attcgtcgcc
     7081 agccaccatc tcacggtgga gccggggtga ccttgagagg gggtcgatca ggctaagcgg
     7141 gcgacgtcga attcgatgca tcggagcgtc tgccgactca caatatttca cacttgttga
     7201 atccaaatgt ctcaagcgag gtatcatacc ggtatgcttc acgacttcag gtttgcattt
     7261 cgacagctgg tgaaggcacg ggcttttacg gcggcggcgg tcatcgtgct cgcgctcggg
     7321 attggcgcga acaccgccat cttcagcctc atgaacacgc tgctcttcca gcggccgcat
     7381 tacacgaagg cggacgagat cgtgcaggtc ttttcgcagg ataagaaaaa cccgaagacg
     7441 tttcgcggct tttcctatcc gacctacctc gatatccgcg accagaacag cgtcttctcc
     7501 ggagtgatgg cgcacaacct ggcgatggtc ggggtgggcg ataaaggaaa tacccggcgc
     7561 gcgtttaccg acgtcgtgag cgccaattat ttctcggtca tgggagtaat gccgatacag
     7621 ggtcgcgcgt tccgggcgga agaggaaaca ccggacacca actcagcggt cgcgatcgtg
     7681 agttacaact attggaagaa ggaaggactc aatccggcaa tgctcggctc gacgatcacg
     7741 atcgacagtc ggcccttcac cgtgatcggg attcttccgc aaggctttac tggcacgacc
     7801 cagatcttcg cgccggaaat ctggctgccg ctcggggttt acgaccaggt cgcgaacgat
     7861 ttcgagacgg agaacaagga caagctcgat gatcgcggag gccgtcaact catgatcgtc
     7921 ggccggctga aaccgggcgt gacagccgcc gcggctgctc cggccttgaa gacgttggcg
     7981 gcgagtctcg agcaggccta tccagtcgag cagaaagatc agacgttcac gctggaaacg
     8041 ctttcccgtt ttgccatcag caccagcccg accagcggca acaatatcaa acgactggcg
     8101 ccgctcctct tcggaatgtc gggtgtcgtg ctgctggtcg cctgcctgaa cctggccaac
     8161 atgctgctcg cgcggggcgc ggcgcggcgc aaagaaatcg cgatccggct ggcgctgggc
     8221 gccagtcgcg gtcggatcgt gcggcaatta ctcgtcgaag gtttcgtcct cgcactggcc
     8281 ggcggaggtt tcggattgct cctgggtctt tggtcgtccg atctcctcgt ggcgtcgtta
     8341 ggcaaaaaaa tgccgatcga tatcgtctgg cagggcggcc tgagcgcgcc ggtgctcgcg
     8401 gcgacgttcg ccttttgcct cctcgggacg ctggccttcg cgctcggacc ggcgctgaag
     8461 ctgtcgaaag ccgcagtcat tggcgatctg aaggaacagg caggcgaaga tacagtcgtt
     8521 aggcgcggga agttttggcc gcgcaatccg ctggtcgtgg tgcagatcgc gttttcgctc
     8581 gcgctgctca ccgcggccgc gctcttcatt cgcggcgcgg gcaaggcggc ggccgtcgag
     8641 accggtttgc agacgagctc gaattacctc ctggaagtcg acgcgagtct cgcgacctac
     8701 gatcaggcac gctcgctcaa tctctatcgc acgctcgagg aacggctcgc cgccctgccc
     8761 ggcgtcgaac gcgcgagcat ctcctccact gttccgttcg ggatggtctc gctcgacaag
     8821 tcggtgcaac gcggcggagc caatccggcg ccggacgcga agccggccac cgccgcggaa
     8881 ggtctcgcgt tcacggcgaa ctggaacagt gtgggtgcgg attatttcaa aaccgtgggc
     8941 ttgcccctgc tgcgcggtcg cgccttcaac atctcggaag cgatgcatca aacgtcaccg
     9001 aaagtcgcga tcattgacga agtgctcgcg aaaaaactct ggcccgatgg cgacgcgctg
     9061 ggccagcgaa ttcaatttgc ggagaagggt ccgactacgg cgaagggtgg cggtgacgga
     9121 acgagagcgc agatcgaggt cgtcgggatc gtgccttacg cgcggaacga tttgttcgag
     9181 aaaaatcctg gcggcatgct ttatctcccc tttgccggtg gtttccagag caacgttttc
     9241 tttcacatca agttcgcgcc caacgccggc cgcgatgtcg gcacgaccac ggatctcatt
     9301 cgtcgcaccg tgcgcgaagt ggatgccgcc gttccggtct tgaccttgaa gacgttcgcg
     9361 caacatctcg agggcaatct cgagctctgg atcgtgcgcg cgggcgcggc gttgttcagt
     9421 acctttggcg ctctggcgtt ggtgctcgcg accgtgggtg tgtacggcgt gaaagcctac
     9481 tcggtcgcgc gtcgcacccg ggaaattggg attcgcatgg cgctgggcgc gcagcgcgag
     9541 acggtgcaat ggatgatcct gcgtgaaggc accgtgatgc tggcgagtgg agtgttgctc
     9601 ggcctgctgt tggcggcggg gactggcaaa ctcattagcg gaatgctcta caacgtcggc
     9661 gcgctcgatc cgctcgcgtt tacgattgca ccgatcgtgc tcgcggcagc agcgttaatc
     9721 gcgacttggc tgccggcccg gcgcgccacg cggatcagcc cgatggccgc gctccgaacg
     9781 gaataggctc cggctgttgg gcgcgtgctt agacgaagag tttcccgcgc tccgggcgct
     9841 tggcagatgt tccggagagc gccccgaaag cgttctgggc tgatgcaggc gtcctcgccg
     9901 tcacgaacat tagactctga ttgatacccg tcttgtccct ttcgacgcta tcctcacctc
     9961 cagggcgcga atagacctcg gttcatcctt cctgcgcgag cgtcgcgtca gcgacctcct
    10021 tcaagagctg acgagcacaa cgaaagcgcc cggcaaactt ctcggaatgc tccttctgga
    10081 gggccggggc aaaattgtcc cggtaacggt gatactcctc gagcgaccgg caatgatact
    10141 gcatcacgta actcggatcg gcgacggcgg agtcgatcac cttgtagacg cgacactcgg
    10201 aaaagcatcc agtccggacg acatcgggaa catgaatgcg cttcatccaa tcgagccact
    10261 ccgactcaat gccggcgtca atggttatcg taacggaata aataaccata ttgtcgcgcc
    10321 tcaatctacc tcacggaatt ggtcccggcg aattgtcttg actcgtattg gtgcagctct
    10381 catgcttgtc ggtcgccggt agccacttgc tactgggatc ttcttatatg ttacagatac
    10441 attcgtt
//
LOCUS       DATMNS010000315         3468 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4580215, whole genome shotgun sequence.
ACCESSION   DATMNS010000315 DATMNS010000000
VERSION     DATMNS010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3468
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4580215"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VNW28_11050"
     CDS             <1..466
                     /locus_tag="VNW28_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HXA10511.1"
                     /translation="AVIAGGARRQDSVHSGLQRVGATAEYVAVHDAARPLVRPEMVEQ
                     IFQAARTHDGAACAAPVSDTLKRAGADRVVTGGVERENLFAVQTPQIFRRDFLTKAYA
                     AVFAAGLEVTDEISAVERAGGKVVLVPNEEPNFKITWPPDLPLAEFVLRTRT"
     gene            complement(484..1464)
                     /locus_tag="VNW28_11055"
     CDS             complement(484..1464)
                     /locus_tag="VNW28_11055"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HXA10512.1"
                     /translation="MSARSAIQSILIIKPSSLGDVVHTLPAAALIRDAYPEADITWVI
                     NPEWAPLLRGNRSIDHVHIFPRGELRGFGMSKHLLPWLRQTARLQPDAALDFQGLLRS
                     ALIGRKSHPREFYGMSDAREGARWFYDHVTPVDRSAHAVERYLALAEDFGARRLHPLR
                     FPLPTGDPIPRFDEDEPFVLLHPFARGRRKSLSDLAVEEFCHALAPRRVVLAGLTKRE
                     MPVPENCVNLLNHTTVLQLIWLIRRARFTVSVDSGPMHIASAITDRLVSIHTWSDPRK
                     VGPYNKDAWVWKNGDLLPVRDLPPPAKRAKGRAFKPADVTPLLPLVSRYL"
     gene            complement(1530..2252)
                     /locus_tag="VNW28_11060"
     CDS             complement(1530..2252)
                     /locus_tag="VNW28_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006978524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXA10513.1"
                     /translation="MRFAILSDIHANLEALQAVLADARAQACTHFVCLGDIVGYNASP
                     GECVKLIQELECPVVKGNHDEQASIVASTEGFNELAEEAIGWTRENLSADAKAWLADL
                     RLTRQVHDFTIVHATLDTPGQWGYIFNDLDAIASFTYQHTALCFFGHTHWPTAFIQDD
                     SVRRIPVGQIVLSAGKKYFINAGSVGQPRDRDWRAAYCIFDTDRQVVEQRRVKYDLET
                     AQKKIRAAGLPDRLADRLAVGR"
     gene            complement(2262..3020)
                     /locus_tag="VNW28_11065"
     CDS             complement(2262..3020)
                     /locus_tag="VNW28_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10514.1"
                     /translation="MTRGALLLLLLLAFGALRLPIESALAQQHRSAYFHEAELGLGLR
                     EQIGQLGFLAALSGFRSLVADVVFIQAHVAWERTQWGRVLLLFREATTLQPRSILFWD
                     MAAWHMAWNASVAALHDPAQPDDLLRRKAQREYVALGKDFLERGIKNNPDRPKLYESL
                     ARLYKEKLNNHAAAARCYAKAAALPGAPEYDKRFAAYELSSAPGEERAAYQALRRLYD
                     RGAHERLPTLLTRLKFLEQELNIPLDQCIPEAAQ"
     gene            complement(3017..>3468)
                     /locus_tag="VNW28_11070"
     CDS             complement(3017..>3468)
                     /locus_tag="VNW28_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10515.1"
                     /translation="ETAGLRPAQVEEAVRGLRASAFSANLLPGIAIIYLKASLLASLT
                     LFVSTFATSNIFTIVVMVCIYLIGHLQSTAREYWLQQHGGGWLAKTFLALVTLAFPDL
                     QLFNLIDDIVAGTAIPAALFAQTAVLGCVYTSIYFFFAWVAFHGKEL"
BASE COUNT          691 a          999 c         1169 g          609 t
ORIGIN      
        1 ggccgtcatc gccggcggtg cgcggcgcca ggactcggtt cactccggcc tgcagcgggt
       61 tggcgccacg gcggagtacg tcgccgtcca cgatgcggcc cggccgctcg ttaggccgga
      121 aatggtcgag caaatttttc aagcggcgcg gacccacgac ggggccgcgt gtgccgcacc
      181 cgtgagcgac acgctgaagc gcgccggtgc ggaccgggta gtgaccggcg gggtggagcg
      241 ggagaatctt ttcgccgtcc agacgccaca aatcttccgc cgcgattttt tgacgaaagc
      301 ctatgccgcg gttttcgcag ccggcctcga ggtaaccgac gagatttcgg ccgtcgaacg
      361 ggcgggcggc aaagtggtgc tcgtgcctaa cgaggaaccg aatttcaaaa tcacctggcc
      421 gcccgatctt ccgctcgcgg aattcgtcct gcgaacgcgt acctgaagcg gctcgtgcag
      481 cgtctacaag tatcggctaa ccagcgggag aagcggtgtg acatccgccg gtttgaaagc
      541 gcggcctttg gcccgtttcg ccggtggtgg cagatcgcgc accggtaaga gatcgccgtt
      601 cttccagacc caggcatcct tgttataggg accgaccttg cgcgggtcgc tccaggtatg
      661 aatcgagacg aggcggtcgg tgatggctga cgcgatgtgc atcgggccgc tgtccacgct
      721 gacggtaaaa cgcgcccggc gaatcagcca gatgagctgc agcacggtgg tgtgattgag
      781 caggttgacg cagttttccg gcaccggcat ttcccgtttc gtcaggccgg cgaggaccac
      841 gcggcgcggc gcgagcgcat gacaaaattc ctcgaccgcc agatcgctga gcgatttgcg
      901 acggccgcgg gcaaagggat ggagaaggac aaacggttca tcctcgtcga agcgcgggat
      961 cgggtcgccg gtagggaggg gaaagcggag cggatggagg cggcgggctc cgaaatcttc
     1021 ggccagcgcg aggtaacgtt cgaccgcgtg cgcgctccga tcgaccggcg tgacgtgatc
     1081 gtaaaaccag cgcgctcctt cccgggcgtc gctcatcccg taaaactcgc gtggatggct
     1141 ttttcgtcca atgagcgcgc tccgcagcag accctggaaa tccagcgcgg catccggttg
     1201 caggcgggcc gtctgccgga gccatgggag caggtgtttc gacatgccga aaccgcgcag
     1261 ctcgccccgc ggaaaaatgt ggacgtgatc gatggagcgg ttcccgcgca ggagcggcgc
     1321 ccattccgga ttgatcaccc aggtaatgtc ggcctcgggg taagcatcgc gaataagggc
     1381 ggccgccggc aacgtgtgca ccacgtcgcc gagcgaactc ggcttgatga tgaggatgga
     1441 ctgaattgct gatcttgcag acatctctcg cggagcgggt ggattgattc ctaatcagcg
     1501 atccgagatc aacaatcagc aatttctgcc taacgaccca ccgccaggcg gtcggcgagg
     1561 cgatcgggca agccggcggc ccgaatcttt ttctgggcgg tctccagatc gtatttgacc
     1621 cgccgctgct cgaccacctg gcggtcagtg tcgaaaatgc aataggcggc ccgccagtcg
     1681 cggtcgcgcg gctggccgac gcttccggca ttgataaaat atttcttccc ggcggaaagg
     1741 acgatctgcc ccaccgggat gcgacgcacg ctgtcgtctt ggatgaaggc agtcggccag
     1801 tgggtgtggc caaaaaagca gagcgcagtg tgctgatagg taaagctggc gatcgcatcg
     1861 agatcattga agatgtagcc ccactgcccc ggagtatcga gggtcgcatg cacgatcgtg
     1921 aagtcgtgca cctgtctcgt taggcggaga tcggccagcc aggctttggc gtccgcggaa
     1981 aggttttccc gcgtccagcc gattgcttcc tcggccagtt cgttgaagcc ttcggtcgag
     2041 gcaacgatcg aagcctgctc atcgtgattc cctttgacca ccggacattc gagttcctgg
     2101 atgagcttga cgcactcgcc cgggctcgcg ttgtaaccga cgatatcgcc gaggcagacg
     2161 aagtgcgtgc aggcctgcgc gcgcgcgtcc gccaggaccg cctgaagcgc ttcgaggttg
     2221 gcgtggatgt cactcaagat ggcaaaacgc atcggaacaa tctactgcgc tgcttcgggg
     2281 atgcactgat ccaacgggat attgagctcc tgttcgagaa attttaagcg cgtgagcaaa
     2341 gtcggcaggc gttcgtgcgc accgcggtcg tagagacggc gcagcgcctg ataagccgcg
     2401 cgctcctctc ccggcgcgga ggaaagttcg taggcggcga agcgcttgtc atactccgga
     2461 gcgccgggga gcgcggccgc tttcgcgtaa cacctggcgg ccgcggcgtg attgttcagc
     2521 ttctctttgt aaaggcgggc gagcgattcg tagagcttcg gacggtccgg gttgttcttg
     2581 atcccgcgct cgagaaaatc cttgccgagc gcaacatact cccgctgcgc tttgcggcgc
     2641 aggaggtcgt caggctgggc cgggtcgtgg agcgcggcga cgctcgcatt ccaagccatg
     2701 tgccaggccg ccatgtccca gaagaggatg gaacgcggct ggagcgtggt cgcttcgcga
     2761 aagagcagga gcacccggcc ccactgggtt cgctcccagg caacgtgcgc ctggatgaag
     2821 acaacgtccg cgacgagtga acgaaaacca ctcaaagccg cgagaaaacc gagctgcccg
     2881 atttgttcgc gcaaaccgag cccgagttcg gcttcgtgaa aataggcgct ccggtgttgt
     2941 tgagcgagag cggactcgat cggcaaacgg agcgcaccga aggccaggag aaggagaagg
     3001 aggagggcgc cgcgggtcat agttccttcc cgtgaaaagc cacccaggcg aagaaaaaat
     3061 agatcgacgt gtagacgcag ccgaggaccg ccgtctgggc aaagagcgcc gctgggatgg
     3121 cggtcccggc gacgatgtca tcgattagat tgaagagttg cagatcgggg aaggcgaggg
     3181 tgacgagggc gagaaaggtt ttcgcgagcc agccgccgcc gtgttgctgg agccagtatt
     3241 cgcgtgccgt cgactggaga tggccgatga ggtaaatgca aaccatcacc acgatcgtaa
     3301 agatattgga cgtcgcaaag gtggaaacaa aaagggtgag cgaggcgagc aaagaagctt
     3361 tcaagtaaat gatggcgatg cccgggagca agttggcgct gaaggccgaa gcccgcagac
     3421 cgcggactgc ctcctcgacc tgggccggac gcaggccggc ggtttcgc
//
LOCUS       DATMNS010000316         2935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4587402, whole genome shotgun sequence.
ACCESSION   DATMNS010000316 DATMNS010000000
VERSION     DATMNS010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4587402"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VNW28_11075"
     CDS             complement(<1..309)
                     /locus_tag="VNW28_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXA10516.1"
                     /translation="MGTKLYVGNLSFNTTETDLQDLFAQAGAVSEVTLMQDKFTGKSR
                     GFAFVTMTNEADAQKAITEFNGKTVEGRPLTVNEARPREARPGGGGGGRGGYGGGGGG
                     Y"
     gene            491..1720
                     /locus_tag="VNW28_11080"
     CDS             491..1720
                     /locus_tag="VNW28_11080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXA10517.1"
                     /translation="MTEQRSNEDEAKRLRVAVVAAGVISPLGAGLEATLTALREARDC
                     VSPVERFPVEECRCTTAGQVEDAWLADVLPNDRRARRLHRASRMMIGALAELLEQAPG
                     FRPQLTVVGTTSGGMSFGEQYYRALQQPGARAAHLPGWIANYPPQKPAVDALEACSLA
                     SPCQVIANACASGTNAIGHAFECIRAGLYDRVLTGGYDALSELVFVGFDSLQAATPDK
                     CRPFDGARNGLVLGEGAALLALENLEHAQARGAKILAEISGYGISTDNHHLTQPHPSG
                     IGPRQAMGRALQSAGRAPASIGYINAHGTATPFNDAAEGKAIAELFDRVPVSSTKGMM
                     GHSLGAAGAIEAVFTILALRSGFLPANINFRVSDLGLDLDIVANKVRAADCAVALSNS
                     FGFGGTNASIVLEALAA"
     gene            1717..2844
                     /locus_tag="VNW28_11085"
     CDS             1717..2844
                     /locus_tag="VNW28_11085"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HXA10518.1"
                     /translation="MSLRLAGLGWVTPLGSTIGEVWTKLLAGVEAKAATLGESLGEKS
                     CRVFPVPAPAIAEAARHPRLRRSSAISRFAAVAGLSALRDAALELNEASAARTALIFG
                     ISNGGVIYTKRFYHEIVKSGAAAASPLLFPETVFNAPASHLAAILGITGASYTVVGDG
                     AVGILALKMAEDLMADPGLDRCLVVGTEEADWLLCDAYRKWRLLRAEPPIEPFARRPK
                     GMILSEGAGAVLLARSGTLEIERIHPGGNFARQREAVGQLDAICRELKGDAPTDLVVA
                     SANGTFIDAAERAAIANHFAAAALYTPKPALGESVAAGAIWQLICAAQALRTQRLPTI
                     LHAPAKLGLTITPPGTRNDWQRAVVTTCGLNQQVAGLALRR"
BASE COUNT          512 a          928 c          974 g          521 t
ORIGIN      
        1 atagccgcca ccaccaccac cgtagccgcc gcgaccgccg ccgccaccac ccgggcgtgc
       61 ttcacgcgga cgcgcttcgt tcacggtcaa gggccggcct tcgaccgtct tgccattgaa
      121 ctccgtgatc gctttttgcg catcggcctc attggtcatg gtgacaaacg cgaagccgcg
      181 cgatttaccg gtaaatttgt cctgcatcag ggtgacttcc gagaccgcgc ccgcctgggc
      241 gaagaggtcc tggaggtcgg tttcggtcgt attgaaggaa agatttccta cgtatagttt
      301 ggtgcccatc tgagtttatt ctttaattga aacgctgctc ggccactgtt cccgcttatc
      361 ggatttcaaa tcgccactga ataaactcaa ctgacaattc accaacgtcc ggacccgcaa
      421 tttctggcgg ctatgtaagg tgaatcggca ggcaaatcaa gcagaataaa cgagggcgat
      481 ttttcgcaaa atgacggagc agagaagcaa cgaggatgaa gccaaacggc tccgggtcgc
      541 ggtcgtcgcg gccggggtaa tcagccccct cggggcggga ctcgaagcga cgctgaccgc
      601 cctgcgggaa gcgcgggatt gcgtctcgcc ggtcgagcgc ttcccagtcg aggaatgccg
      661 ctgtaccacg gccggacagg tggaggacgc ctggctggcg gacgttttgc ctaacgaccg
      721 gcgggcgcgc cgcctgcatc gcgcctcgcg catgatgatt ggcgcactgg cggaattgct
      781 tgagcaggcg ccggggttcc gcccgcaatt gaccgtcgtc gggacgacca gcgggggaat
      841 gagtttcggc gaacaatatt accgcgcgct ccagcagccc ggggcgcgcg cggcgcacct
      901 gccggggtgg atcgccaatt acccgccgca aaagccggcg gtcgatgcgc tcgaggcctg
      961 cagcctcgcc tcgccctgcc aggtcatcgc gaacgcctgc gcctccggca cgaacgcgat
     1021 cggccacgcc ttcgagtgca tccgcgcggg gctctacgac cgcgtcctaa cgggcggcta
     1081 cgacgcgctc tcggaattgg tatttgtcgg gttcgattcg ctgcaagcgg ccacgccgga
     1141 caagtgccgg ccctttgacg gcgcgcgcaa cggcctcgtc ctcggggaag gcgcggcgct
     1201 cctcgcgctc gagaatcttg aacacgcgca ggcgcgcggg gcgaagatcc tcgcggaaat
     1261 cagcggctac ggcatctcga cagacaatca tcatctcacc cagccacatc cgtcgggcat
     1321 cggcccgcgc caggcaatgg ggcgtgcgct gcaaagcgcg gggcgggcgc cggcgtcgat
     1381 cggttacatc aacgcgcacg gcaccgccac cccgttcaac gatgcggcag agggaaaagc
     1441 aatcgccgag ttgttcgacc gcgtgccggt tagctcgacc aaggggatga tggggcactc
     1501 cttaggcgca gccggcgcga tcgaggcggt gtttaccatc ctggcgctgc gcagcggttt
     1561 tctcccggcc aatatcaact tccgcgtctc ggatctggga ctcgatctcg acatcgtggc
     1621 gaataaagtg cgcgcggccg actgcgccgt ggcgctttcg aattcgtttg gcttcggcgg
     1681 cacgaacgct tccatcgtac tcgaagcgct ggcggcatga gcctgcgcct cgccggcctc
     1741 ggctgggtta cgcccctcgg aagcacgatc ggcgaagtct ggacgaagct gctcgccggc
     1801 gtggaggcga aagcggccac gttaggcgag tcgttaggcg aaaagagctg ccgcgttttt
     1861 cccgtcccgg cgccggcgat cgccgaggcc gcccggcatc cacggcttcg gcgctcgagc
     1921 gcgatctcgc gcttcgcggc ggtcgcgggg ttgagcgcgt tgcgcgacgc cgcgctcgag
     1981 ctgaatgaag cgagcgcggc gcggaccgcc ctgatttttg ggatttcgaa tggcggggta
     2041 atctacacca agcgctttta tcacgagatc gtgaagtcgg gtgcggccgc agccagcccg
     2101 ctcctgtttc cggagacggt tttcaacgcg ccggccagcc atctggcggc cattctcggc
     2161 atcaccggcg ccagttacac ggtggtcggc gacggcgcgg tcgggattct ggcgctgaag
     2221 atggcggagg atttgatggc cgacccgggg ctggatcgct gcctggtggt ggggacggag
     2281 gaggccgact ggttgctctg cgatgcctat cgcaaatggc gcctcctccg cgcggaaccg
     2341 ccgatcgagc cgttcgcacg gcgacccaaa ggcatgatcc tgagcgaagg cgccggcgcg
     2401 gtgttgctcg cgcgcagtgg gacgttggaa atcgaacgca ttcatccggg cggaaatttt
     2461 gcgcgacagc gcgaagccgt cgggcaactc gacgcgattt gccgggagtt aaaaggcgac
     2521 gcgccgacgg acctcgtggt cgcgagcgcg aacggcacct tcatcgacgc cgccgagcgc
     2581 gcggcgatcg cgaaccattt tgcggccgcg gcgctttaca cgccgaaacc ggcgctcggc
     2641 gagagtgtgg ccgcgggcgc catctggcag ttgatctgcg ccgcgcaggc gttgcgcacg
     2701 cagcgattgc cgacgattct gcatgcgccg gcgaagcttg gcctgacgat cacgccgccg
     2761 gggaccagga atgactggca gcgggccgtc gtaacaactt gcggactgaa tcaacaagtc
     2821 gccggactcg cgctgcgtcg ctgatcctcg tcgtgcttgc gatcgatcgt ttcgattacg
     2881 attcacgagc tggagccgga atggcaaaag ggttttcact ttgcaaccgc ttcgt
//
LOCUS       DATMNS010000317         3319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3913461, whole genome shotgun sequence.
ACCESSION   DATMNS010000317 DATMNS010000000
VERSION     DATMNS010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3319
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3913461"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="VNW28_11090"
     CDS             complement(<1..520)
                     /locus_tag="VNW28_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXA10519.1"
                     /translation="MPNFLENTFTQLQQASDHVVLREIRGTEFVSVSGAELLDQVARA
                     RAYLRGAGLQAGARCALLGPNSIRWAAIDLALMAEGLVVVPLYSRQAPAELVAMMKDC
                     TPRLLITSDAALAESVSQVWLEAPPRATFDEILQSAPLQKGIAGPPNPRQGEDLVTII
                     YTSGTSGEPKGVC"
     gene            complement(526..1965)
                     /locus_tag="VNW28_11095"
     CDS             complement(526..1965)
                     /locus_tag="VNW28_11095"
                     /inference="COORDINATES: protein motif:HMM:NF016145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide pyruvyl transferase family
                     protein"
                     /protein_id="HXA10520.1"
                     /translation="MTDFLLVAWVSAMIEFRRFSWMFGIGKRWTPGEKLKLLFAGYNG
                     TRNTGSDVRVQETLRQIRHVLGAHNVDLSVMTQSFDRTQGYFEGSRQVHLPDVFPPFL
                     FREVHANHGVVACEGSMFKSKFANALTTMMIGSLGLASAENKLSVAYAGDAGHMDDLI
                     EWMCARYTRESLVITRSLESQQLLSGLGVPNELGADTAWTFEPRPPDFARNTLRKAGW
                     DEKTPILVLCPIHPFVWPVRASIAKYIARVTTGAYRDSQYRTVYFFESGAEVDRKFNH
                     YVAGYAQAAKAFLQRHKVFPILVAMERLDAVACRAIEKEIPGTPIFTSDDYDMFELVS
                     ILRACTYMVSSRYHGIVTCMPAGVVSAGVTMDERIRNLMRERGHQHLLLNVDDPDLGP
                     KLLDVMEKLVVEADSIRDSIGRTVVSNLKAMSRMAVFFEDEIRKTYPEFPLRKGVLSW
                     EDNLPPFSDNLRKLVEQYDSSPAVLAAGR"
     gene            complement(1962..3119)
                     /locus_tag="VNW28_11100"
     CDS             complement(1962..3119)
                     /locus_tag="VNW28_11100"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXA10521.1"
                     /translation="MAIFLTGSTGYLGSYLVAGLFTGHADRLNLLVRAKTEQEARERL
                     WQSLQLHFTFPEFLEHLNSRVRIFRGDLTSERFGLSDDEYHALVDSTDSILHCAASLN
                     RKSEKQCLNVNLRGSLEVIQLARRAQNRHGLRRYSHVSTVAVAGKRQNEVVSEDGSID
                     WNRSDYDPYARTKKFSEHMVHQLLPDVTRTIFRPAIVLGDSRRPETSQFDMVQAFDTL
                     ARFPVLPLRPNDKIDIVPANYVGAAIVKIHQMEKPNCAIYHLSSGTGAQTYKELTDAL
                     AEIGGWSKPSYQPWLGGPFSKTVNWLAFKGGPVGYGASLLKVFWPYLDWNTVFDNSRV
                     VQEMGEAPAKFSTYAYPLLQFSRANKFKYPAKSWPAGAGKSVVVSASGAGV"
     gene            complement(3137..>3319)
                     /locus_tag="VNW28_11105"
     CDS             complement(3137..>3319)
                     /locus_tag="VNW28_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10522.1"
                     /translation="KMFDFSPETIDWWDYWINIHIPALRRWCYPLMEGKPLEAREPRA
                     LNWSAPPEEAASAAIR"
BASE COUNT          663 a          852 c         1166 g          638 t
ORIGIN      
        1 gacaaacgcc cttgggttcg ccggaagttc ctgaagtgta gatgatggtg acgagatctt
       61 cgccttggcg gggattgggc gggccggcaa ttcccttttg caacggcgcg gactgaagga
      121 tttcgtcgaa ggtggcgcga ggcggggctt cgagccagac ttgtgaaaca ctttcggcga
      181 gggcagcatc gctcgtgatc agcaggcgcg gggtgcaatc cttcatcatg gcgacgagtt
      241 cggcgggtgc ctggcgggaa tagaggggta cgacgacgag gccctcggcc atgagagcga
      301 gatcgatcgc ggcccagcga atggagtttg gcccgagcaa ggcgcagcgg gcgccggcct
      361 gcagaccggc gccacgaaga taggcgcggg cgcgggcgac ctgatcgaga agttccgcgc
      421 cggagacgct cacgaactcc gtgccgcgga tttcgcgcaa aacgacgtgg tcggaggcct
      481 gctgcaattg cgtaaacgtg ttttcgagaa aattgggcat cggcttcagc gccctgccgc
      541 cagaacggcg ggagaggaat cgtattgctc cacgagcttt cgcagattgt cgctgaacgg
      601 cggcaagttg tcttcccagc tcaggacgcc tttgcgcaag ggaaattcgg gataggtctt
      661 gcgaatttcg tcctcgaaga aaacggccat gcgcgacatg gccttgaggt tggaaacgac
      721 ggtgcggccg atggagtcgc ggatggagtc agcttcgacg actagctttt ccatgacgtc
      781 gaggagcttg gggccgagat ctggatcgtc gacgttgagc agcaggtgct ggtggccgcg
      841 ctcgcgcata aggttgcgta tgcgctcgtc catggtgacg ccggcagaga cgaccccagc
      901 gggcatgcag gtgacgatgc cgtgataacg cgaggagacc atgtaggtgc acgcgcggag
      961 aatcgagacg agttcgaaca tgtcgtaatc gtcggacgtg aaaatcggcg tgccgggaat
     1021 ttccttttcg atggcgcggc aggcgactgc gtcgagacgt tccatggcca cgaggatcgg
     1081 gaatactttg tggcgttgca ggaaagcttt ggcagcttgc gcgtaaccgg cgacgtagtg
     1141 gttgaacttg cgatcgactt cggcgccgga ttcgaagaaa tagacggtgc ggtactggct
     1201 gtctctgtaa gcgccggtgg tgacgcgagc gatgtacttg gcgatggaag cgcgcacggg
     1261 ccagacgaag ggatgaatcg ggcaaagaac gaggatggga gttttttcgt cccacccggc
     1321 tttgcgcaac gtattgcggg cgaagtcggg cgggcgaggt tcgaaggtcc aggcagtgtc
     1381 ggcgccgagt tcgttgggga cgccgaggcc ggagaggagt tgctgcgatt cgaggctgcg
     1441 cgtgatgacc agcgattcgc gggtgtagcg cgcgcacatc cactcgatga gatcgtccat
     1501 gtggccggca tcgccggcgt aggcgacgga aagcttgttc tccgcggaag cgaggccgag
     1561 cgagccgatc atcatcgtgg ttagcgcgtt ggcgaatttg ctcttgaaca tcgagccttc
     1621 gcaggcgacg acaccgtgat tggcgtggac ttcgcggaaa aggaacggag gaaagacgtc
     1681 ggggaggtgc acctggcgcg agccctcgaa atagccctgg gtgcggtcga aactctgggt
     1741 catcacgctg aggtcgacgt tatgcgcgcc gaggacgtga cggatctggc gcagggtttc
     1801 ctggacgcgg acgtcgctgc cggtgttgcg tgtgccgttg tagccggcaa agagcagctt
     1861 gagcttttcg ccgggcgtcc agcgcttgcc gatgccaaac atccaactga agcgccggaa
     1921 ctcgatcatg gcgctgaccc aggcgacgag gaggaaatcg gtcatacgcc ggctccgctg
     1981 gcgcttacga cgacgctctt gcccgcgccc gccggccagg attttgcggg atatttgaat
     2041 ttgttggcgc ggctgaactg caagagcggg tacgcatagg tggaaaactt tgcgggcgct
     2101 tcgcccattt cctgtacgac gcgggaatta tcgaacacgg tgttccaatc gagatacggc
     2161 cagaaaactt tcagcaagga agctccatag ccaaccggcc cgcccttgaa ggccagccag
     2221 ttcacggtct tggagaaagg cccgccgagc cacggctggt agctgggctt ggaccatccg
     2281 ccaatttcgg ccagcgcatc ggtgagttct ttatacgtct gcgcgccggt accagaagat
     2341 agatgataaa tggcgcaatt cggtttttcc atttggtgaa ttttaacgat agccgcgccg
     2401 acgtagtttg cggggacgat atcaatttta tcgttggggc gtagcgggag cacgggaaag
     2461 cgcgcgaggg tatcgaaggc ctgcaccatg tcgaattgcg aggtttcggg ccggcgggag
     2521 tcgccaagca caatggcggg gcggaaaatc gtgcgggtca cgtcggggag aagctggtgg
     2581 accatgtgct cggagaattt cttggtgcgg gcataggggt cgtagtcgga gcggttccaa
     2641 tcgatggaac cgtcttcgct gacgacttca ttctggcgct tgccggcgac ggcgacggtg
     2701 gaaacgtggg aatagcggcg caggccgtgg cgattctggg cgcggcgggc gagctggatg
     2761 acttcgagcg agccgcgaag attcacgttc aggcactgct tttcggattt gcgattcaaa
     2821 gaggcggcgc agtgaaggat ggagtcggtg gaatcgacca gcgcgtgata ctcgtcgtca
     2881 ctgagaccga agcgctcgct ggtgagatcg ccgcgaaaga tgcgcacacg ggagttcagg
     2941 tgttccagga attcgggaaa ggtgaagtgg agttgcaacg attgccagag gcgttcgcgg
     3001 gcttcctgtt cggttttcgc gcggacgagg agattcaggc gatcggcatg gccggtgaag
     3061 agacccgcca cgaggtagct gcctagatat ccagtggaac ctgtcaggaa aatagccata
     3121 gacggatgac ggcgcttcag cggatcgccg ccgacgcagc ttcctccggt ggcgccgacc
     3181 agttgagcgc gcgcggctcg cgagcctcga gcggcttccc ttccatcaag gggtagcacc
     3241 agcggcggag agccggtatg tggatgttaa tccagtagtc ccaccagtca atggtttccg
     3301 gcgaaaagtc gaacatctt
//
LOCUS       DATMNS010000318         9648 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3914042, whole genome shotgun sequence.
ACCESSION   DATMNS010000318 DATMNS010000000
VERSION     DATMNS010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 9648)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9648)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9648
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3914042"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="VNW28_11110"
     CDS             complement(<1..880)
                     /locus_tag="VNW28_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXA10523.1"
                     /translation="MPQFSYRARNAQGGLVEGVLNVADRAIAIQQIEQQDCVPIRIEA
                     IATGPQVLDGKATVAAAPPAQNLKIPHGQLLIFTEQLGHLLQAGMTLDESLGVLEKRL
                     KHPRVQQMTHALHQALVDGRSFSQALRDFPRIFPALYVNMVGAGEASGALPEILTRVV
                     EHLMQTKNLRDRVQQALIYPAFLALAGSALIIIFITFMVPQLTNFMAQTGGSLPLPTR
                     VLVGANHVITGYWWVFVLVVIGGAVGFRAFVRSDEGRLTWDRFRLAIPGYGRVRQHQY
                     YAQFSRTLGTLMENGIP"
     gene            954..1829
                     /locus_tag="VNW28_11115"
     CDS             954..1829
                     /locus_tag="VNW28_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXA10524.1"
                     /translation="MRTAFEKIELHSGTTVAVSEYGLPSGAPVFFCHGWPSSRTMAEL
                     TDAAARELNLRIISPDRPGIRDSSLQPDRKLLDWPPLLAELADQMGIATFRILGISGG
                     APYALVAAWALPERVSAIAVVSGAPPLADLTDRSGLLALYRWLLFFYPRHRGLLRFFF
                     HAARPFLSLKLPIRLRPLLLQFLQPCDANVMRDVAAFEACFESQRQAWRASADGLMVD
                     AEIYAQPWGFPLGEIRVPVRLWHGRKDRSFHWQLAQSLGARLPYCSAHLVDQEGHYSL
                     PIRHMREILEDLRSA"
     gene            1880..2953
                     /locus_tag="VNW28_11120"
     CDS             1880..2953
                     /locus_tag="VNW28_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXA10525.1"
                     /translation="MEDFTGSTTRDPPVRRNSNLGPEFEARVHRLVADWGAEKSPELI
                     EELIATALKMARDKIGTGDLKLMNRSLKELRYAAKIFAPYRDVRKVVVFGSARTAATD
                     PEAQLAEEFGRQMVANKYMVITGGGDGIMGAAQRGAGRENSFGLNIRLPFEQRANQVI
                     EGDQKLINFNYFFTRKLNFVKETHAFVLFPGGFGTMDEGFEALTLMQTGKALIIPIVL
                     IDRPGGSYWETWMRFLTDHLLKQGLVSAEDFNFIKIAHNVPAAVEEILLFYRNYVSSR
                     WVGSQLVIRICQPLSRKAVAALNKQFAEVLRTGEITQGGPLPQERNQPEIAHLTRLVL
                     TPHRDGFGRYRQLIDAINLSETA"
     gene            complement(3088..3360)
                     /locus_tag="VNW28_11125"
     CDS             complement(3088..3360)
                     /locus_tag="VNW28_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXA10526.1"
                     /translation="MPEPDLRTQIKEMLVKNLMLPTPADQIADDLPLFGPGGLGLDSI
                     DALELVVSMEKTFGVGVPNSEVAGRALQTVNSIHDYIIEKTERPAA"
     gene            complement(3673..3822)
                     /locus_tag="VNW28_11130"
                     /pseudo
     CDS             complement(3673..3822)
                     /locus_tag="VNW28_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492443.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
     gene            complement(4116..5540)
                     /gene="glnA"
                     /locus_tag="VNW28_11135"
     CDS             complement(4116..5540)
                     /gene="glnA"
                     /locus_tag="VNW28_11135"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HXA10527.1"
                     /translation="MPNNKKTPADVSKMIKDHEVKLVDFKFTDLPGSWQHFTTTLTEY
                     NEDIFTEGLGFDGSSIRGWKAINASDMLVIPDSASAWVDIFNAESTLSLICTIVEPIT
                     REPYSRDPRGVAEKAEAYLQSTGIADTAFFGPEAEFFIFDDVRFSNAGNSSSYSVDSN
                     EGHWNSGREEFPNLGYKIRAKEGYFPVAPSDTLQDIRNEMCLELENAGIPIERQHHEV
                     ATAGQAEIDIRFAPLKTMGDYMQYYKYIVRNVARKHNKTVTFMPKPLFGDNGSGMHTH
                     ISLWKDGKPLFAGNGYAGLSDMALFFIGGILKHAPALTAITNPTTNSYKRLVPGFEAP
                     VNLAYSARNRSAAIRIPTYSANPKSKRIEFRTPDAAANPYVAFSALLLAGLDGIQNRI
                     DPGDPLDKNLYELPPEELAGVPSVPDSLGGAIAALAADHAFLLRGDVFNADFITNWIE
                     MKQKEYDALRLRPHPFEFQMYYDV"
     gene            complement(5707..6045)
                     /locus_tag="VNW28_11140"
     CDS             complement(5707..6045)
                     /locus_tag="VNW28_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXA10528.1"
                     /translation="MKKLEAIIKPFKLEEVKEALAELGIEGMTVTEVKGFGRQKGHTE
                     IYRGSEYTVDFLPKVKVEVVLADDMVDKAVSVVVSAAKTGKIGDGKVFVLPVEHAVRI
                     RTEEIGEKAV"
     gene            6167..>9648
                     /gene="dnaE"
                     /locus_tag="VNW28_11145"
     CDS             6167..>9648
                     /gene="dnaE"
                     /locus_tag="VNW28_11145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXA10529.1"
                     /translation="MPADSFVHLHLHTEYSLLDGAVRMKELMKKAAEMKMPAVAITDH
                     GNLFGAIEFYQAATAAGLKPIIGVEAYLAPGSIRDRPNSQRDAASHFTLLARDEAGYR
                     NLVRLMSTAHLEGMHYKPRIDRELLAAHSEGLIGMSACLKGEINLAIQADQLDKARKS
                     AATFRDILGAENFFLELHDHGLEAQRKCNRVLPQIAREFGLGLVAANDVHFLERSHHE
                     AHDVMICIGTGKMVHDEKRMRYVPELYFKSADEMRALFPDHPEAISNTVAIAERCNLK
                     LEFGSSKFPEYEPPAGRTRREYLRELCEAGLRQRYGERATHDPELRARLTKELEVIEK
                     TGFVSYFLIVWDFIHFARQRGIPVGPGRGSAAGSLIAYVLEITDIDPLQFGLQFERFL
                     NPERVSPPDIDVDFCESRRGEVLEYVRQKYGERRVSQIITFGKLKAKSVVRDVGRVMG
                     LSYGDCDRIAKMIPNELNITLAAAAEKTPELKRAIETEPATRQLWEYAGLLEGLSRNA
                     GVHAAGVVIGDRDLSEYVPLCRDSKGNDIVSQYAMGPLNDLGLLKMDFLGLKTLTVIE
                     DTLTLIRQRDPDFSLKQIPLDDAASFALYNRGETIGLFQMESGGMTSVSKQMDVQRLD
                     DIMALIALYRPGPMDTIGDYIERKRGLKKIRYAHPLLEEVCADTYGVMIYQEQVTAAA
                     NRLAGFSLGQSDLLRRAMGKKDKGKMAKERASFVEGCARTNKIPEKKANAIFDLLEKF
                     AGYGFNKSHSAAYGVISYQTAYLKAHYPVEFMAGLLSNEINNTDKISVFVGECKRMGI
                     PILPPDMNRSGLKFTPEGRASARPGSSSDAASETGRAEARPSEAIRYGLAAIKNVGEG
                     AMAAAIAERERGGDFTSLEDFCSRLDSRLANRKIIESLVKAGAFDFLGRDRAELFACI
                     EETLAASASSHRDRAAGQVSLFGDLPHPTISRGNREYAQWTGREKLSFEKELLGFYVT
                     GHPLDAYAPLLAGGKYQTIASLGELADRASFTIAGAITQVDRKFTKKEGKPFAVVFLE
                     DLTGTLEVVLWNETYVNVSHALEPGTVIAIRGTADKRDDNLRATAQKVKVLTPEAVAS
                     VPNGVAPGSETNGAAHAREDAPILLRFAAGIGADELRAVREILASSPGSQPVTLEMTS
                     FDGETVRIEAGEACR"
BASE COUNT         1862 a         2938 c         2921 g         1927 t
ORIGIN      
        1 acgggattcc gttctccatc aaggtgccca gggtgcggga aaactgcgcg taatattgat
       61 gctggcggac acggccgtaa cccggaatgg ccaggcgaaa tcgatcccag gtcagccgtc
      121 cctcgtcgct ccggacgaaa gcacggaacc caacggcgcc gccgatcacg acgagcacaa
      181 agacccacca ataaccggtg attacatggt tggcgccaac caagacccgc gtcggcaagg
      241 gcaaactgcc cccggtttgc gccatgaaat tcgtcaactg cggcaccatg aaggtgatga
      301 aaatgatgat caacgccgaa ccggcgaggg ccagaaacgc cgggtaaatc agcgcctgct
      361 gaacgcggtc gcgcagattc ttggtctgca tcaaatgttc gacgacgcgc gttagaatct
      421 cgggcaacgc gccgctcgcc tcgcccgcgc ccaccatgtt cacgtagagc gccgggaaga
      481 tgcgcgggaa atcccgcagc gcctgcgaga agctgcgacc atccaccagc gcctggtgca
      541 gcgcatgcgt catctgctgg acgcgcggat gtttcaggcg tttctccagc acgccgaggc
      601 tctcgtcgag cgtcatgccg gcttgcagga ggtggccgag ctgctcggtg aaaatcagca
      661 gctggccatg gggaattttg agattttgcg cgggcggcgc ggccgccacc gtggctttgc
      721 catcgaggac ctgcggccca gtggcgatgg cttcgatccg aatcggaacg caatcctgtt
      781 gctcgatctg ctggatcgcg atcgcgcgat ccgcgacatt gagcacgcct tcaacgaggc
      841 caccctgggc gttgcgggcg cggtaggaaa attgcggcat tgatcgaaag tgggcgctga
      901 aattaacccg ctgcgtcgcc gccgtccaat cgttgcgcta cgttcgcgga ccgatgcgaa
      961 cggcattcga aaaaattgag ctgcacagcg gcacgacggt ggccgtgtcc gaatatggcc
     1021 tgccatccgg cgcgccggtt ttcttttgtc acggctggcc cagctcgcgc acgatggcgg
     1081 agctgacgga tgcggcggcg cgggaactca atttgcggat catttctccc gaccggcccg
     1141 ggataagaga ttcttcgctg cagcctgatc gcaaattgct cgactggccg ccgctcctcg
     1201 ccgagctggc cgaccagatg gggatcgcca cgtttcgcat tctcggaatt tccgggggcg
     1261 ctccctacgc cctggtcgcg gcttgggccc tcccggagcg cgtcagtgca atcgcggtcg
     1321 tgagcggcgc gcccccgttg gccgacctca ccgaccgcag cgggctgctc gcgctttatc
     1381 gctggctcct ttttttctat ccgcgtcatc gcgggctgct ccgctttttc tttcacgccg
     1441 cgcgcccgtt cctttccctg aaacttccga ttcgtttgcg cccgttgctc ctccagtttc
     1501 tgcaaccgtg cgatgccaac gtgatgcgcg acgtggccgc cttcgaggcc tgctttgaaa
     1561 gtcagcgcca ggcctggcgc gcctcggccg atggtctgat ggtggacgcg gaaatctacg
     1621 cgcaaccgtg gggatttccg ctcggggaga tccgggtgcc ggtgcgtctc tggcatggcc
     1681 ggaaggatcg cagttttcac tggcagctcg cccagtcgtt aggcgcgcgc ctcccgtatt
     1741 gcagcgcgca cctggtcgac caggagggcc attattcgct gccgattcgc cacatgcgcg
     1801 agatcctcga agatctgcgc tccgcctaac gtcggcagtt ttccctgttc gtcggcgcga
     1861 aaatcgctca attgacaggg tggaagattt tacgggaagc acgacgcggg atccgccggt
     1921 ccgccgcaat tcgaatctcg ggccggagtt tgaggcccgc gtccatcgcc tggtggccga
     1981 ctggggcgcg gagaaatctc cggagttaat cgaggaattg atcgccaccg ctctcaagat
     2041 ggcgcgcgac aaaataggca ccggcgacct gaagctgatg aaccgctcgc tcaaggaact
     2101 gcgctacgcc gccaagatct ttgccccgta tcgcgacgtg cggaaagtgg tcgtcttcgg
     2161 ttcggcccgg accgcggcga ccgaccctga ggcgcagctc gccgaggagt ttgggcgcca
     2221 gatggtggcg aacaaataca tggttatcac cggcggcggc gatggcatca tgggcgcggc
     2281 gcagcgtggc gccgggcggg aaaatagttt cggtctaaat attcgtctgc ccttcgagca
     2341 gcgcgccaat caggtcatcg aaggcgatca gaagctgatc aattttaatt acttcttcac
     2401 ccgcaaattg aacttcgtga aggaaacgca cgccttcgtg cttttccccg gcggcttcgg
     2461 gacgatggac gaaggcttcg aggcgctcac tctcatgcag acgggcaaag cgctcatcat
     2521 tcccatcgtg ctgattgatc ggccgggcgg cagctattgg gagacctgga tgcgcttcct
     2581 caccgatcac ctgctcaagc agggtttggt ttccgcggag gatttcaatt tcatcaagat
     2641 cgcgcacaac gtcccggcgg ccgtcgagga gattctgctc ttctatagaa actacgtctc
     2701 ctcccgttgg gtcggatctc agctggttat tcggatctgc cagccgttat ccaggaaagc
     2761 ggtcgccgcg ctcaacaaac aattcgcgga ggtgctgcgc acaggtgaaa tcacgcaggg
     2821 cgggccgttg ccacaagagc gaaaccaacc cgagatcgcg catttgaccc gtttggtcct
     2881 gacgccgcac cgggacgggt tcggtcgtta ccggcaattg atcgatgcga tcaacctgtc
     2941 ggaaacggcc tgatgttgac gcattgctct gcgaagcgcg gcacagagcg ccgctcgcat
     3001 cttaaaccgc cgcgccaaat atttcctttc ggttgccccc gctggcggca gcgcccagct
     3061 cgcgccgcgc ggcgcgaagc gtccacatca ggccgccggg cgctcggtct tctcgatgat
     3121 gtaatcgtga atcgagttga ccgtctggag tgcccgcccg gcgacttccg aattcggcac
     3181 cccaacgccg aaggttttct ccatgctgac gacaagctcg agcgcatcga tggaatcgag
     3241 gccgagaccg ccgggcccga agagcggcag gtcgtcggca atctggtctg ctggcgtcgg
     3301 cagcatcagg ttcttgacca gcatctcttt gatttgagtg cgaagatcgg gttcgggcat
     3361 ggccactttt tggggacgct atgcttagac gaaaagcccg ccgaagtcat cgcctaaaaa
     3421 tgcgcgctgc gggaattgtg cgccgtccga tgaagcgcgg ctgcgtctcg cgccgcccac
     3481 agggaggcgg ctacgtgtag atgcttcgcg gtgcgaagcg cgcgcccaaa ctcgccgcgt
     3541 tcaggcgtcc tgcgaagtcc gctcttccgg cgccacttcg cgcgccgtcg gcgcattgtc
     3601 gatccgcggc acggtcgcat ccgcctgacg aacgtccggc ttcgctggcg tggtggtccc
     3661 ggcgttgtgc agttcgtcgc tgaactcgtc cttcgccttc tggaattcct tgaccgcctg
     3721 gcccatccct ttcgcgagtt cgggcagctt cttcgcgccg aaaagcacga ggacgatgag
     3781 caggatgatg atcaggtccg ggccgccaag attagaaagc aaggcgagga agtgcatgat
     3841 gaagcttaac atccgcccat cgccgcgggt tgcaaggcga tttacccggg gcgctatacg
     3901 ccttgaaaat cagggatgca ctgcggacaa atcaagtttc ctgcctcgga aatccgaata
     3961 tcgaaacccg aaatccgaga caattcattc aaattccgaa aactgggaaa ttatcaaaac
     4021 ccggacattt tctttttctg aaattgagca tcgtttgaat ttgcttcgga tttcggtatt
     4081 cggatttcga atttcgggtc tccgccggag caccgctaga cgtcgtaata catctggaac
     4141 tcgaacgggt gcggacggag ccggagggcg tcgtattcct tctgcttcat ctcgatccaa
     4201 ttggtgatga agtcggcgtt aaacacgtcg ccgcggagga gaaaggcgtg gtcggccgcg
     4261 agcgccgcga tcgctccccc taacgaatcc gggacgctcg gcacaccggc caattcctcc
     4321 ggcggaagct cgtagaggtt cttgtcgagg ggatcgcccg gatcgattcg gttttggatc
     4381 ccgtcgaggc ccgcgaggag cagggccgag aaggcgacat acggattagc cgccgcatcc
     4441 ggggtgcgga attcgatgcg tttcgatttc ggattcgccg agtaggtcgg gatgcggatc
     4501 gcggccgagc gattccgcgc ggagtaggcg agattaaccg gcgcctcgaa gccgggcacg
     4561 agccgtttgt agctgttcgt cgtcgggttg gtaatcgcgg tcagggccgg cgcgtgtttc
     4621 aaaatgccgc cgataaaaaa gagcgccata tcgctcaatc cggcgtagcc gttgccggcg
     4681 aagagcggtt tcccgtcttt ccagagcgag atgtgggtgt gcatgcctga gccgttgtcg
     4741 ccgaagagcg gcttcggcat gaaggtaact gtcttgttgt gcttccgcgc cacgttgcgc
     4801 acgatgtact tatagtactg catgtaatcg cccatcgtct tgagcggcgc gaaacggata
     4861 tcgatctcgg cctgacccgc ggtcgcgacc tcgtgatgct ggcgttcgat cgggatgccg
     4921 gcattctcga gctcgagaca catctcgttg cggatgtcct gcagcgtgtc cgacggcgcg
     4981 accgggaaat aaccttcctt cgcccggatt ttgtaaccca ggtttgggaa ttcttcccgt
     5041 ccgctgttcc agtgcccctc gttgctgtcg accgagtagg aggaactgtt cccggcgttg
     5101 ctgaaacgca cgtcgtcgaa gataaaaaac tccgcttccg gcccgaagaa cgcggtgtcg
     5161 gcgatgcccg tgctttgcaa atacgcctct gctttttccg ccaccccgcg cggatcgcgg
     5221 ctgtagggct cgcgcgtgat cggttcgacg atcgtgcaaa tgaggctgag cgtcgactcg
     5281 gcgttgaaga tatcgaccca cgcgctcgcc gaatccggaa tgacgagcat gtcggaggca
     5341 ttgatcgcct tccaaccgcg aatgctcgag ccgtcgaacc cgaggccctc ggtgaaaata
     5401 tcctcgttgt attcggtcag ggtggtcgtg aaatgctgcc aggacccggg cagatcggta
     5461 aacttgaagt ccacgagttt gacctcatgg tccttgatca tcttgctgac gtcggcggga
     5521 gttttcttat tgttaggcat ggtggttggt gaatgacgaa tgaggaatga ggactgagga
     5581 atgaccaatg acgaagcccg aacagcctgc ggcgaggtta ttcgggcttc aacaccagtg
     5641 cgaactcgat cattcgacct tcgtcattga ttcggatttc gtcattcgtc cttcgccatt
     5701 tcccgctcac accgccttct caccgatctc ttcggtccgg atgcggaccg cgtgttccac
     5761 cggcaacacg aaaactttgc catcgccgat cttgccggtc ttcgccgcgc tcaccaccac
     5821 gctcaccgcc ttgtccacca tgtcgtcggc gagcacgact tccactttta ctttcggcaa
     5881 aaaatcgacg gtgtattcgc tcccgcgata aatttccgtg tgccctttct ggcgaccgaa
     5941 gcccttcact tccgtcaccg tcatgccctc gattccgagc tcggcgagcg cttccttcac
     6001 ttcttcgagt ttgaatggct tgatgatcgc ttcgagtttt ttcatgggcg ttatagtggt
     6061 gtcgggctct tttcaatcgg actggtggta gaaaatcaga gattaagggt gcagcgcaag
     6121 cgccgatttc ttgccgctgc cggcaccgag tttatagtgc ggtcgcatgc cggccgactc
     6181 ctttgttcat ctccacctgc acaccgagta ttcgctcctc gacggcgcgg tgcggatgaa
     6241 ggagctgatg aaaaaggcgg ccgaaatgaa aatgccagcg gtcgcgatca ccgaccacgg
     6301 caacctgttt ggcgcaattg aattttatca ggcggcgacg gccgccggcc tcaagccgat
     6361 catcggggtc gaagcctatc tggcgccggg atcgatccgg gaccgaccga attcccaacg
     6421 cgatgccgcg tctcatttta ccctcctcgc ccgggacgag gcgggctatc gcaatctcgt
     6481 taggctgatg tcgaccgcgc atctcgaggg gatgcattac aaaccgcgga tcgaccggga
     6541 actgctcgcc gcgcattccg agggtctgat cgggatgagc gcctgcctca agggcgagat
     6601 caacctggcc atccaggccg atcagttgga caaggcgcgg aagagcgccg cgacttttcg
     6661 cgacatcctg ggcgcggaaa atttcttcct cgagctgcac gatcacggac tcgaagcgca
     6721 gcgcaaatgc aatcgcgtcc tgccgcagat cgcgcgtgaa ttcgggctcg gtttggtggc
     6781 ggcgaacgac gtgcatttcc tcgagcgcag ccatcacgaa gcgcacgatg tcatgatttg
     6841 catcggcacc ggcaagatgg tgcacgacga gaagcggatg cgttatgtgc ccgaacttta
     6901 tttcaaaagc gcggacgaaa tgcgcgctct tttccccgat catccggagg cgatttccaa
     6961 cacggtcgcg atcgccgagc gctgcaacct gaagctggaa tttggcagct cgaagttccc
     7021 cgagtacgag ccgccggcgg gccggacacg ccgggaatat ctgcgcgaac tttgcgaggc
     7081 cggcctgcgc cagcgctacg gagagcgggc gacgcacgat cccgaattgc gcgcccgcct
     7141 aacgaaagag ctggaggtga tcgagaagac cggcttcgtc agctacttcc tcatcgtttg
     7201 ggacttcatc cactttgcca ggcagcgcgg cattccggtc ggcccgggcc gcggttcggc
     7261 ggcgggttcg ctcatcgcct atgtgctcga aattaccgac atcgatccgc tccagtttgg
     7321 cttgcagttc gaacgcttcc ttaatccgga gcgcgtttcg ccgccggata tcgacgtcga
     7381 cttctgtgaa tcgcgccgcg gcgaagtgct cgaatacgtg cggcaaaagt atggcgagcg
     7441 ccgggtttcc cagatcatca ccttcggcaa actgaaagcg aaaagtgtgg tgcgcgatgt
     7501 cgggcgcgtg atgggcctga gctacggcga ttgcgaccgg atcgcgaaga tgatccctaa
     7561 cgagttgaac atcactctcg ccgcggccgc cgagaaaacg ccggagctga agagagccat
     7621 cgagaccgag ccggcgaccc gccagctctg ggaatacgcc ggcttgctcg aaggcctttc
     7681 gcgcaacgcc ggggtgcacg ccgccggcgt cgtgatcggc gaccgcgacc tctcggagta
     7741 cgttccgctc tgccgcgact cgaagggcaa cgatatcgtc agccaatacg cgatgggccc
     7801 gctgaacgat ctcggcctgc tgaaaatgga tttcctcggc ctcaagacgc tcaccgtgat
     7861 cgaggacacg ctcactttga tccgccagcg cgacccggat ttttcgctca agcagatccc
     7921 gctcgatgac gcggccagct ttgccctcta caaccgcggc gaaacgatcg gccttttcca
     7981 gatggaatcg ggcgggatga cgagcgtctc gaagcagatg gacgtgcaaa ggctcgacga
     8041 catcatggcc ttgatcgcgc tctaccgccc cggcccgatg gatacgatcg gcgactacat
     8101 cgagcggaaa cgcggcctca agaaaattcg ttatgcccac ccgctcctcg aggaagtctg
     8161 cgccgacacc tacggggtga tgatctacca ggaacaggtg accgcggcgg ccaaccgcct
     8221 cgccggtttt tcgttaggcc aatccgacct cctccggcgc gcgatgggga aaaaggacaa
     8281 gggcaagatg gcgaaggagc gtgcgagttt cgtcgagggg tgcgcgcgca cgaacaagat
     8341 tcccgagaaa aaggcgaacg cgattttcga tctgctggaa aaattcgctg gctacgggtt
     8401 caacaaaagc cacagcgccg cctacggcgt gatcagctat cagacggctt atttgaaggc
     8461 gcactacccg gtcgaattca tggccggttt gctgagcaac gagatcaata ataccgacaa
     8521 gatttcggtc ttcgtcggcg agtgcaaacg gatgggaatc ccgatcctcc cgcccgacat
     8581 gaaccggagc ggactcaagt tcaccccgga gggacgtgct tccgcacgtc cgggttcttc
     8641 ttcggatgca gcttcagaga ctggacgagc cgaagcgcgt ccctccgagg ccattcgtta
     8701 cggactcgcc gcaatcaaga acgttggtga aggcgcaatg gccgccgcca tcgcggaacg
     8761 cgagcgggga ggggacttca cctcgctcga ggatttttgc agccggctcg attcgcgcct
     8821 cgccaaccgc aagatcattg agagcctggt caaagccggg gccttcgatt ttctcgggcg
     8881 cgatcgggcc gaacttttcg cctgtatcga agaaacgctc gccgcctcgg cctcttctca
     8941 tcgggatcgc gccgccggcc aggtttcgct tttcggagat ctccctcatc cgaccattag
     9001 ccgcggaaat cgcgagtatg cgcaatggac ggggcgcgag aaattgtcct tcgagaaaga
     9061 actgctcggc ttctacgtca ccggccatcc cctcgatgct tacgccccgc tcctggccgg
     9121 cggaaaatat cagaccatcg cgtcgttagg cgagctggcc gatcgcgcga gcttcaccat
     9181 cgccggcgcc atcacccagg tggaccggaa gttcaccaag aaagagggga agcctttcgc
     9241 tgtggtcttc ctcgaggacc tgaccggaac tctcgaggtc gtcctttgga acgaaaccta
     9301 cgtcaatgtt tcccacgcgc tcgagcccgg gacggtgatt gcgatccgcg gcacggccga
     9361 caagcgcgac gacaacctcc gcgcgaccgc gcaaaaggta aaagtgctaa cgccggaggc
     9421 cgtagcctcc gtgcccaatg gcgtggcgcc cggctccgag acgaacggag ccgcgcacgc
     9481 gcgcgaagac gcacccatcc tgttgcgctt cgccgcggga atcggcgcgg atgaattgcg
     9541 agcggtgcgc gaaatcctgg cctcttcccc cggctcacag cccgtcaccc tggaaatgac
     9601 gagcttcgac ggcgaaacgg tgcggatcga agccggcgaa gcctgccg
//
LOCUS       DATMNS010000319         2209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4257097, whole genome shotgun sequence.
ACCESSION   DATMNS010000319 DATMNS010000000
VERSION     DATMNS010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4257097"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..498
                     /locus_tag="VNW28_11150"
     CDS             22..498
                     /locus_tag="VNW28_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10530.1"
                     /translation="MSRSLQRFSLLVSLCFLASCQTMPEGIEQARLAMSAKIQTEPPG
                     NYFIGRRYYKPAFHFWGYIRRPGQPWSTAQLVMLNENQKLAPDREQVNFGFDNNFEYK
                     LYGRFSGDKIYEPASNGIYPEFVLTGYELISTNPAPIFKSQYRGSGDNQYQIEKPE"
     gene            584..1513
                     /gene="xerA"
                     /locus_tag="VNW28_11155"
     CDS             584..1513
                     /gene="xerA"
                     /locus_tag="VNW28_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HXA10531.1"
                     /translation="MLHRAKRLHLTTERPAKDRLVEEFLGFLEVERNASPRTLRIYRD
                     ALRDFQAQTKAPGWRQCRAHHFRDYLFALLKRKQARSYVRLQFSALRSFYRFLVARNR
                     LTKDPVREIQLPKAAQQLPLVLTQTQIGELLAAPLQVRKPKSAPVWMPSRDAAILEVF
                     YSSGLRLAELASLNVNDLDLYTESVRVLGKGRKERVCPVGAPALQAVSRYRAAANVHS
                     GALFLNKSRRRISPRSIWLILKRYLRHTSIPIALSPHQLRHSFATHLLDNGADLRSVQ
                     ALLGHVSLSTTQIYTHVTTERLKKAYAEAHPRA"
     gene            complement(1615..>2209)
                     /locus_tag="VNW28_11160"
     CDS             complement(1615..>2209)
                     /locus_tag="VNW28_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXA10532.1"
                     /translation="KAHPDVMHLLLQILEEGKITDSLGQKIDFRNTIIIMTSNVGAEL
                     IKRQTTMGFGAQRDEANYDVMRDKILEETKRVFKPEFLNRLDEIIVFHTLSKPDLMRI
                     VDLEVNKVKRRLRLKEIEITLDDAAHEFVIEKGYDPTYGARPMRRAVERYLEDPLAEE
                     LLRGGVKAGDQVQVSVENAKLVFKVAEPHDNAAASAS"
BASE COUNT          419 a          719 c          627 g          444 t
ORIGIN      
        1 ccggcgccgc cgtaaaaaat aatgtctcgc tcactgcaac gtttcagcct tctcgttagt
       61 ctttgctttc ttgccagttg ccagacgatg ccggaaggca ttgagcaggc gcggctcgcg
      121 atgtccgcga agatccagac ggagccgccg ggtaactact tcatcggccg gcgctattat
      181 aaaccggcct ttcatttttg gggctacatc cgccggcccg gccagccctg gagcacggca
      241 cagctcgtga tgttaaacga aaatcagaag ctcgcgcccg atcgtgagca ggtgaatttc
      301 ggcttcgaca acaacttcga gtacaagctt tatggtcgct tcagcggcga caaaatttat
      361 gagccggcga gcaatggcat ctatccggaa tttgtgctca cgggctacga gctgatctcg
      421 accaacccgg cgcccatttt caaaagccaa taccgcggct cgggtgacaa ccagtaccaa
      481 atcgaaaagc cggagtaggc gcggcgcgag ggacgcgtct ggcagacgtc tccctctggg
      541 aggcgcggga acggaggctg cttccccggc gcagtctcgg gccgtgctgc acagagcgaa
      601 gcgtctccac ctaacgaccg agcgtccggc gaaagacagg ctggtcgagg aatttctcgg
      661 cttcctcgag gtggagcgca acgcttcgcc ccgcaccttg cgcatctatc gggacgcact
      721 gcgcgacttt caggcccaga cgaaggcccc gggctggcgc caatgccggg cgcatcactt
      781 ccgcgattat ctcttcgcgc tgctcaagcg gaaacaggcg cgctcctacg tccggctgca
      841 attttccgcc ctgcgcagct tctatcgctt cctcgtcgcg cgaaatcgcc taacgaaaga
      901 tccggtgcgg gaaattcagc tgccgaaagc ggcccagcaa cttccgctcg tcctgacgca
      961 gacccagatc ggcgaacttc tcgccgcccc actccaggtc agaaaaccga aaagcgcgcc
     1021 cgtctggatg ccgtcgcggg atgccgcgat cctcgaggtt ttttacagca gcggtttgcg
     1081 cctggctgag ctcgcctcgc tcaacgtcaa cgatctcgac ctctacacgg agtcggtccg
     1141 tgtcctcggc aaggggcgga aggaacgtgt ctgcccggtg ggagcaccgg cgctgcaggc
     1201 ggtctcccgt tatcgggccg ccgccaatgt gcacagtggt gcgctctttc ttaataaatc
     1261 acgccggcgc atttcgccgc gctcgatctg gttgattctg aaacgctatc tccgccacac
     1321 ctcgatcccg atcgcgctca gcccccacca actccggcac agcttcgcga cccacctgct
     1381 cgataacggc gccgacctgc gcagcgtgca ggcattgtta ggccacgtca gtctttcgac
     1441 cactcagatc tacacccacg tcacgaccga gcgcttgaaa aaggcctacg cggaggcgca
     1501 cccccgagcc tgacgacgag cgcccgcccg gtagacgttt cgcggtgcga aacgcgaggg
     1561 tgacgcccca attgcagcgg gatctgcgcc tcccacaggg aggcgtctac agaatcagct
     1621 cgcgctcgcg gctgcgttgt cgtgcggttc ggcaaccttg aaaacaagct tcgcgttttc
     1681 gaccgacacc tgcacctggt cacccgcttt cacgccgccc cggagcagct cttccgcgag
     1741 cggatcctcg agatagcgtt ccactgcgcg ccgcatcggg cgcgcgccgt aggtcgggtc
     1801 gtagcctttc tcgatcacga attcgtgggc cgcgtcatcg agcgtaatct cgatttcctt
     1861 caaccgcagg cggcgcttca ccttgttcac ctcgaggtcg acgatccgca tcaggtccgg
     1921 tttgctgagg gtgtggaaaa caatgatctc atcgagccgg ttcaggaact ccggcttaaa
     1981 gacgcgcttc gtctcctcga gaatcttgtc ccgcatcaca tcgtagttcg cctcgtcgcg
     2041 ctgcgcgccg aagcccatcg ttgtctggcg cttgatcagc tcggcgccga cattcgaggt
     2101 catgatgatg atcgtgtttc ggaaatcgat cttttggcca aggctgtcag tgattttgcc
     2161 ctcttccaga atctggagca ggagatgcat gacatccgga tgagccttt
//
LOCUS       DATMNS010000320         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4260307, whole genome shotgun sequence.
ACCESSION   DATMNS010000320 DATMNS010000000
VERSION     DATMNS010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4260307"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..2581
                     /gene="ppk1"
                     /locus_tag="VNW28_11165"
     CDS             356..2581
                     /gene="ppk1"
                     /locus_tag="VNW28_11165"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HXA10533.1"
                     /translation="MPTSASSSKPEPPANVVAMPAPNEPAPKARFSDPKNFINRELSW
                     LEFNRRVLEEAQDPTQPLLERVKFLGIVSSNLDEFFEIRVAGIKQQIASETSDVGPDG
                     MSPTETFVAIQRVVHEMVAQQYALWNKEIEPLLEQNNIRLHHVGELSAKRAAWARRYF
                     QEEVFPMLTPLAVDASHPFPQLLNKSHNLLVRARARKGGEPMHAIVQVPRAVSRLIHI
                     PRGDKADESWNYIYLASLIKQHVADLFPGLVLDGVHAFRVTRNSDLYVDEEEAENLLR
                     SIEQELRRSSRGNAVRLEVEADCPNDFSNLLLKFFQLTEADLYKLDGPLSMTHLAPLV
                     ANDAFAKLKDRVFQPTRDPALPAHADIFEAMRKQDILLHHPYESYDPVVELIESAATD
                     PLVLALKITLYRTSGDSPIVEALIHAANEGKQVTALVELRARFDEAANIHWARRLEES
                     GAHVVYGVVGLKTHCKALLIVRRDPDRLRQYVHLGTGNYHPRTARIYTDFSLFTTAPE
                     LTEEVAIVFNTLTGLAGYPGLKKLLVAPFDLQPRLIGLIERERDLAAAGQPGRIVAKL
                     NSLVDHEIIEKLYEASCAGVKIDLIIRGICCLRPKIPGLSENIRAISIVGRFLEHSRI
                     YYFGNAGKPDLYLASADWMPRNFFRRVEIAFPIESPPLRDEIINEVLPRFLTDYGKAR
                     ELQADGSYVRLQPEEGAPRSQAQMQFREHSRRQAKKLAEKQREGKMRLSPIQHPPPER
                     A"
     gene            2578..3273
                     /locus_tag="VNW28_11170"
     CDS             2578..3273
                     /locus_tag="VNW28_11170"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXA10534.1"
                     /translation="MKKKILVVDIGGSNVKLMISAEEKRRKFPSGPKLSPNEALRQIR
                     EVAADWDFEAVSIGFPAPVRDGKILSDPKHLGKGWAGFDFAKALGKPVRLINDAALQA
                     LGSYRGGGRMLFLGLGTGLGSALTWEGHVLSLELGELPFGDDGVIEDWLGDSGLEALG
                     RKYWQRETLYAISQLKLALIADRVVLGGGNAKFLEKLPDGFELGHNRNAYPGGCRLWE
                     TDPETKRPRWRVM"
     gene            3448..>3822
                     /gene="thyA"
                     /locus_tag="VNW28_11175"
     CDS             3448..>3822
                     /gene="thyA"
                     /locus_tag="VNW28_11175"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HXA10535.1"
                     /translation="MRQYQDLLRLVLEKGRPKEDRTGTGTLSVFGAQARFDLRETFPL
                     LTTKKLHLKSIIHELLWFLRGDTNVSYLQERGVTIWNEWADEAGDLGRVYGAQWRDWR
                     GPDNQHIDQIERVIAQIKTNPDS"
BASE COUNT          786 a         1223 c         1132 g          681 t
ORIGIN      
        1 tcgaagggat ggatgccgat ctggttgtgc tcacgccgga actgaaagtg gtccgcacct
       61 tctgcggcgg tcctccgcag accagttagc gcgcaccaaa aaccactccc tgcgtctatt
      121 ccgtggagaa aatccgcgca aaaatttgcc gctccgccga attcaaatcg gacaccgccc
      181 agtaatccat ccgccgcgcg gtccactgca cgagattgca tccgcgcagg gaaacgttcg
      241 attgagcaga agcggcgctc tcgccgggcg aaacgtcttc actggcgagt tgcgcaaagc
      301 ggaggcgaag ggttagttac cgctccccgc ggacggaccg cgggcccttc tcgttatgcc
      361 gaccagcgcc agttcgtcca agcccgaacc accggccaac gtcgtggcca tgcccgcgcc
      421 taacgaaccg gcaccgaagg cgcggttctc cgatccgaaa aatttcatca accgcgaact
      481 gagctggctc gagttcaatc gccgcgtcct cgaggaagcg caggacccga cccagcctct
      541 cctcgagcgg gttaagtttc tcgggatcgt gagctcgaat ctcgatgaat ttttcgagat
      601 ccgcgtcgcc ggcatcaagc agcagatcgc gagcgagacg agcgatgtcg gccccgatgg
      661 gatgagcccg acggaaactt ttgtcgccat tcagcgggtc gtccatgaga tggtggcgca
      721 gcagtacgcg ctctggaaca aggagatcga gccgctcctc gagcaaaaca acattcgcct
      781 ccaccacgtc ggcgaattga gcgcgaaacg cgcggcctgg gcgcggcgtt attttcagga
      841 ggaagttttt cccatgctca caccgctggc ggtggacgcg agccatccgt ttccgcaact
      901 cctgaacaag agccacaacc tgctcgtgag ggcgcgggcc aggaaaggcg gcgaaccgat
      961 gcacgccatc gtgcaggtgc cgcgcgcggt ttcccgtttg atccacatcc cgcggggcga
     1021 caaggccgac gagagctgga actacattta tctcgcctcc ctcatcaaac agcacgtggc
     1081 cgatcttttt cccggcctcg tcctcgacgg ggtgcacgct tttcgcgtga cccggaacag
     1141 cgatctctac gtcgacgagg aagaagcgga aaacctcctc cgcagcatcg agcaggaact
     1201 gcggcgctcg agccgcggca acgcggtccg gctggaggtg gaagccgatt gccctaacga
     1261 cttctccaat ctgctcctca agttcttcca gttgaccgag gccgatctct acaagctcga
     1321 tggtccgctt tcgatgaccc acctcgcgcc gctggtcgcg aacgacgctt ttgcgaaact
     1381 gaaggaccgc gtcttccaac cgacccgcga tccggcgctg cccgcgcacg ccgatatttt
     1441 cgaggcgatg cgcaagcagg atatcctgct ccatcatccc tacgaaagtt acgacccggt
     1501 ggtcgagctg atcgaaagcg cggcgaccga tccgctcgtc ctcgcgctca agatcacgct
     1561 ctaccggacg agcggcgatt cgccgattgt cgaggcgctg atccacgccg ccaatgaggg
     1621 caaacaggtg acggcgctgg tcgagctgcg ggcgcgcttc gatgaagcgg cgaacattca
     1681 ctgggcccgg cgcctggagg aatccggggc gcacgtcgtc tacggcgtgg tcggcctgaa
     1741 aacccactgc aaggcgctgc tcatcgtgag gcgcgacccc gatcgcctgc gccagtacgt
     1801 ccatctcggg accggcaatt atcatccgcg cacggcgcgc atttacaccg acttcagcct
     1861 cttcacgacc gcgcccgagc tgaccgagga agtggcgatc gtctttaata ccctcaccgg
     1921 tctggccggt tatcctggcc tgaaaaaact tctcgtcgcg cccttcgacc tgcaaccgcg
     1981 tctcatcggc ctgatcgagc gcgagcgcga cctcgccgcc gcgggccagc ccgggcggat
     2041 cgtggccaag ctcaactcgc tcgtcgacca cgagatcatc gaaaaactct acgaagcttc
     2101 ctgcgccggg gtgaaaatcg atctcatcat tcgcggcatt tgctgtctcc gcccgaaaat
     2161 cccgggactg agcgaaaaca tccgggcgat cagcatcgtc ggtcgttttc tcgagcacag
     2221 ccggatttac tatttcggca acgccggcaa acccgatctt tatctggcga gcgccgactg
     2281 gatgccgcgc aactttttcc ggcgggtcga aatcgcgttc ccgatcgagt cgcctccgct
     2341 gcgcgatgag atcatcaacg aagtgttgcc gcgtttcctg accgactacg gcaaggcgcg
     2401 cgagctgcaa gccgacggca gttatgtccg cctccagccg gaagaaggcg cgccgcgttc
     2461 gcaggcccag atgcaatttc gcgagcactc gcggcgccag gcaaaaaagc tggcggagaa
     2521 gcagcgcgaa ggcaaaatgc ggctctcgcc catccagcat ccaccgccag agcgcgcatg
     2581 aagaagaaga tcctcgtcgt cgacatcggc ggatcgaacg tcaagctgat gatctcggcg
     2641 gaggaaaaac ggcggaaatt cccgtccggg ccgaaacttt cgcctaacga agccttgagg
     2701 cagatcaggg aggtcgcggc ggactgggat ttcgaggccg tctcgatcgg ttttcccgcg
     2761 ccggtgcgcg acgggaaaat tctgagcgac ccgaaacatc tcggcaaagg ctgggcggga
     2821 ttcgattttg cgaaggcgct gggcaagccg gtgcgactga tcaacgacgc cgccctccag
     2881 gcgctcggga gctatcgcgg cggcgggcgg atgctttttc tcgggctcgg caccggtctc
     2941 ggctcggccc tcacctggga aggtcacgtc ctctcgctcg agctgggcga gctgcccttc
     3001 ggcgacgacg gggtcatcga agactggctc ggcgacagcg ggctggaagc cctgggccgg
     3061 aaatactggc agcgcgaaac cctctacgcg atcagccaac tgaagctcgc gctcatcgcc
     3121 gatcgcgtcg tgctcggggg cgggaacgcg aagttcctcg aaaaactgcc cgacggtttc
     3181 gagctcggcc acaatcgcaa tgcctacccc ggcggttgcc ggctctggga aaccgatccg
     3241 gaaaccaagc gtccgcgctg gcgcgtcatg taggtgggaa aatcaaaatc cgaacctcga
     3301 aatccgaaac aaatttggaa ttaacagaaa tctcaagatg caaacccagc ctggtcaccg
     3361 ggtgagtttt ggtttcggtt tcctgggaat ttctacattc ggatttcctt cgaatttcga
     3421 atttcggatt tcggatttcg cgtagccatg cgccaatacc aggatcttct ccgactcgtc
     3481 ctcgaaaaag ggcgcccgaa ggaagaccgc accggcacgg gaaccctttc cgttttcggg
     3541 gcgcaggcgc gcttcgacct gcgggagacg ttcccgctcc tgacgacaaa aaagctccac
     3601 ctcaaatcga tcatccacga gctgctttgg tttttgcgcg gcgacacgaa cgtcagctac
     3661 ctccaggaac gcggagtcac gatctggaac gagtgggcgg acgaggcggg cgatctcggc
     3721 cgcgtctacg gcgcgcaatg gcgcgactgg cgcgggccag acaatcagca catcgaccag
     3781 atcgagcgcg tcatcgcgca aatcaaaacg aatcccgaca gt
//
LOCUS       DATMNS010000321         5225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_3921826, whole genome shotgun sequence.
ACCESSION   DATMNS010000321 DATMNS010000000
VERSION     DATMNS010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5225
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3921826"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VNW28_11180"
     CDS             <1..299
                     /locus_tag="VNW28_11180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10536.1"
                     /translation="LLRDYATDYEKIDHRQITEEIIGQFYRGQFGLASFEHSQRFDFA
                     RARGRLLSSSYAPDATHARHAAMIAELRRIFETHQTKNEVVFDYETKVYYGRLG"
     gene            475..1389
                     /locus_tag="VNW28_11185"
     CDS             475..1389
                     /locus_tag="VNW28_11185"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HXA10537.1"
                     /translation="MSATLERSETKPVTQRSVFIFRFASTIVLWSVALLIVFSGYELA
                     FYGLISFVGLIALWEFYGMLDHKGLPNFKITAMICGAVMLCGSFYYFAKYGPARSYDF
                     ELAALLFFLLTVFTRQMFASLRDDEPLQTMAYTLFGLLYVLWLYNFITKIVYVTPRAA
                     NGAVTGQFYVFYCIAITKFSDMGAYLTGSVIGRHPLVPHISPKKTWEGFFGALAFSLL
                     ASVGLFKLMPGHLSVLTLTHSIVLGLLLGFAAVIGDLAESIIKRSTGVKDSGNMLPGI
                     GGALDLIDSLLFTAPLLFFYLRLVIRVP"
     gene            1628..2044
                     /locus_tag="VNW28_11190"
     CDS             1628..2044
                     /locus_tag="VNW28_11190"
                     /inference="COORDINATES: protein motif:HMM:TIGR04256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXA10538.1"
                     /translation="MNKMDGMGEAAAYDLCGQIIGLATKVHSKLGQGFLESVYRNSLA
                     LELRKAGFNVELERPINVYYEGELVGAFIADILVNETTIVELKAVQVLAKVHEVQLVN
                     YLTSTGLDDGVLLNFGGPRLEYKKKFRVSKAVPVSL"
     gene            2221..3432
                     /gene="dxr"
                     /locus_tag="VNW28_11195"
     CDS             2221..3432
                     /gene="dxr"
                     /locus_tag="VNW28_11195"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HXA10539.1"
                     /translation="MKRKRVVVLGATGSIGESALKVARDIPERMEIVGLAAKNNARKL
                     AAQANLVRPEAVCLVDEDKIAELRGALEYEPRIFSGAAGLEEIACLTNADMVLVAVVG
                     TGGLRPALAAIEAGKDLAVASKEILVMAGEAVMGAAARKGARVLPVDSEHNAIFQCLE
                     SGRGLGAPMGGGATRNRDTKVPPTLVRRIILTASGGPFRETPNGQFAAITPEQALKHP
                     TWNMGPKVTIDSATLFNKGLEMIEAHWLFGVEMARVEVIIHPQSIVHSMVEFADGSVL
                     AQLSHSDMCFPIQYAVTWPDRVPNSLPPLDFGKLQKLEFAAPRLEAFPALNLARRAGE
                     TGGTLPAVMNAANEVAVSAFLDRRIRFPRIWQLVEEVMNRHASVANPSLDAILEADRW
                     ARDEAAGRATD"
     gene            3454..4866
                     /gene="rseP"
                     /locus_tag="VNW28_11200"
     CDS             3454..4866
                     /gene="rseP"
                     /locus_tag="VNW28_11200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HXA10540.1"
                     /translation="MLLHALRIIFILLEVIVLFNLLIVVHELGHFLAARWRGLVIEKF
                     GVWFGKPLWKKTINGVQYSLGSLPFGGFVALPQLAPMDIIEGETETDRAKLPPISAFD
                     KIIVAIAGPLFSMLLALAFAGVVWAVGHPVSEADMTTVIGYVEKNSPGEKAGLQAGDK
                     ILEVDGQAVTRFAGMNDSVMWNVIRSEGESIPFVVERDGRRLSLSIVPYKAPSSGWRR
                     KSVRQVLIYPATTPVIDQVQPKTPAAAAGLQHGDIILGYDGTRIYSPISLAAYVETHA
                     GQPIALAVERGGQELKINVTPELLPNEKTPRIGIAWDTSGRMSIAHPNPIEQVYNSVT
                     STLETIGAVASPKSDVKLQHLSGPLGIVRIYYLLFESDRGWQLALWFSVILNVNLAIL
                     NMLPIPVLDGGHILLALVEAVRRKPVNIRVLEIVQTGCAVVIIGYIAYISFFDVGDWL
                     GEKRGSDDAPATAVQASPTP"
     gene            4920..5165
                     /locus_tag="VNW28_11205"
     CDS             4920..5165
                     /locus_tag="VNW28_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10541.1"
                     /translation="MDVKEIRELYETAPFQSFDLFPPNGSKVQIDHPEFIAFSRDYRT
                     LHFYPLEGGARHIDVKLITEVRVPSSNGSKPPRKKKR"
BASE COUNT         1029 a         1480 c         1591 g         1125 t
ORIGIN      
        1 atctcctgcg cgattacgcg acggattatg aaaagatcga ccaccgacag atcaccgagg
       61 aaatcatcgg ccagttctac cgtggtcagt ttgggctggc ttccttcgag cacagtcaac
      121 gctttgactt tgccagggcg agagggcggt tgctctcttc ctcctacgcc ccggacgcca
      181 ctcacgcacg gcacgccgcc atgatcgcag agctcagaag aatcttcgag actcaccaga
      241 cgaagaacga agtcgtattc gactacgaga cgaaagtcta ctacggccga ttgggctgaa
      301 gaaagatttc ccccagggtc ggttccggga tttcgctgtg accgcgtgag cgttcgctgc
      361 tgtccggcca ggggagtggc ggtcgcgagg cgttgctgcc gcgcagaaaa gcgcgcggcg
      421 cagcctgact ccgaggtgga caaatttctc tctggcgggg atgatgcagg ctgcatgtcc
      481 gccacgcttg aacgcagcga aaccaaaccg gtgacgcaac gttccgtttt tattttccgc
      541 tttgccagca cgatcgtcct ctggtcggtc gcgctcctga ttgttttctc aggatacgaa
      601 ttggcgttct acggattgat cagcttcgtc ggcctgatcg cgctctggga attttacggg
      661 atgctcgatc acaaggggct gccgaatttc aaaatcacgg cgatgatctg cggcgccgtg
      721 atgctttgcg gcagttttta ttacttcgcc aaatatgggc ccgcgcgctc ctacgacttc
      781 gaactggcgg ccctgctctt ttttttgctg accgttttca cccggcaaat gttcgcgagc
      841 ctgcgcgatg acgagccgct ccagacgatg gcctacacgc tcttcgggct cctttacgtg
      901 ctttggcttt ataacttcat caccaagatc gtctacgtga cgccgcgcgc ggcgaacggg
      961 gcggtgaccg ggcagtttta tgttttttac tgcatcgcga tcacgaaatt cagcgacatg
     1021 ggcgcgtatt taaccgggag cgtgatcggc cggcacccgt tggttccgca cattagcccg
     1081 aagaaaacct gggaaggatt tttcggcgcg ctcgcctttt cgttgctcgc cagtgtcggt
     1141 cttttcaagc tgatgccggg gcacttgtcg gtgctgactc tgacgcattc gattgtgctc
     1201 gggttactcc tcgggttcgc cgcggtcatc ggcgatctgg ccgaatcgat catcaagcgc
     1261 agcaccggcg tgaaggattc cgggaacatg ctgccgggaa tcggcggcgc gctcgatctc
     1321 atcgacagcc ttttgtttac tgcgccgctt ttgttcttct atctgcggct ggtgattcgc
     1381 gtgccgtgat tttggacaga atgaaccgaa tggacggaat gagccaggtg ggattcgggg
     1441 attgtccttg accctctttt attctgcaaa ttcggtacat tctgtcagag aaactgacgg
     1501 agggtttgga cagaatgaac aaaatggacg gaatgaggga ggcggggttc ggtgattgtc
     1561 tttaaccctc ttctattctg taaattcggt acattctgtc agagaaactg acggagattt
     1621 ggacagaatg aacaaaatgg acggaatggg cgaagcagcg gcttacgatc tttgcggtca
     1681 gatcatcggg ctcgcgacga aggtccattc caaactcggg cagggatttc tcgagtcggt
     1741 ttaccggaac tcactggccc tcgagctgcg caaggcgggc tttaatgtcg agctggagag
     1801 accaatcaac gtttactacg agggcgaatt ggtgggcgcg ttcatcgcgg acattctcgt
     1861 gaatgaaacg acgatcgttg aattgaaggc cgttcaggtg ctggcaaaag ttcacgaggt
     1921 tcaactcgta aattatttga cgtcgacggg tctcgacgac ggagtgctgc tcaatttcgg
     1981 cgggccgcgc ctcgagtata agaagaaatt tcgggtgtcg aaagcggtgc cggtttcgct
     2041 ctgacatccg gcccgggatt cccgacagca tgcacagaat caagagagcc cgatgatttt
     2101 ggacagaatg aacagaatgg acggaataaa ggaggcggga ttcgggcatc gcctttaact
     2161 cttctatttg aatttcctgt ccttttccat tcggttgatt ctgttcattc tgtccgaaag
     2221 atgaaacgga aacgcgtagt cgtgctcggc gcgacgggct cgatcggcga gagcgcgctg
     2281 aaagtggcgc gcgatatccc ggagcgaatg gagatcgtcg ggctcgcggc caaaaacaat
     2341 gcgcggaagc tggcggcgca ggcaaatctt gtgcgcccgg aagcggtttg cctggtcgat
     2401 gaagacaaga tcgcggaatt gcgtggcgcg ctcgaatatg agccgcggat tttttccggc
     2461 gcagccgggc tggaggaaat cgcctgcctg acgaacgcgg acatggtgct ggtggcagtc
     2521 gtcgggacgg gcggcttgcg gccggcgctg gcggcgatcg aagcgggcaa ggacctggcg
     2581 gtggcgagca aggaaatcct cgtgatggcg ggcgaggcgg tgatgggcgc ggcggcgcgg
     2641 aagggagcgc gcgtcttgcc ggtggatagc gagcacaacg cgatctttca gtgcctggaa
     2701 agtgggaggg gccttggtgc cccgatgggc ggcggggcca ccaggaatcg ggacacaaag
     2761 gtccctccca cattggtcag gcgcatcatt ctgaccgcgt ccggcgggcc ttttcgggag
     2821 acccctaacg ggcagtttgc cgccattacc ccagagcagg cgttaaagca tcccacctgg
     2881 aacatggggc caaaggtcac gatcgattcg gccacgcttt tcaataaggg actagaaatg
     2941 atcgaggcgc actggctctt cggcgtggag atggcgcggg tggaagtaat cattcatccg
     3001 cagagcatcg tgcactcgat ggtggaattc gccgacggct ccgtcctcgc acaactctcc
     3061 cattccgata tgtgctttcc gattcagtac gccgtcacct ggccggatcg cgtgccgaat
     3121 tctctcccgc cgctggattt cgggaaactc cagaaactgg agttcgccgc gccccgtttg
     3181 gaggcttttc cggcgctcaa tctcgcccgc cgcgcgggcg aaacaggcgg cactctgcct
     3241 gctgtaatga acgcggccaa tgaagtcgct gtctctgctt ttctcgatcg tcggattcgg
     3301 tttccacgca tctggcaact ggtcgaggaa gtcatgaatc gccacgcctc cgttgccaac
     3361 ccgagcctcg atgccatatt ggaggccgac cgctgggcgc gcgacgaagc cgccggccgc
     3421 gctaccgatt aacgtccgcc aattccgagc gccatgttat tgcacgccct acgtatcatc
     3481 ttcatccttc tcgaggtcat cgtcctcttt aacctcctca tcgtggtcca cgaactgggt
     3541 cacttcctgg ccgcgcgctg gcgcggactg gtcatcgaaa agttcggcgt ctggttcggg
     3601 aaaccgctct ggaagaaaac gatcaacggc gtccagtatt cgttaggctc gctccccttt
     3661 ggcggttttg tcgcgctccc gcagctcgcg ccgatggata tcatcgaggg cgaaaccgag
     3721 accgaccggg ccaagctgcc gccgatttcg gcgttcgaca aaatcatcgt ggcgatcgcc
     3781 ggcccgctct tcagcatgct cctcgcgctc gcctttgccg gagtcgtctg ggcggtcggt
     3841 catccggtga gcgaagccga catgacgact gtgatcggat acgtggaaaa aaattcgccg
     3901 ggcgaaaaag ccggcctgca ggcgggcgac aagattctcg aggtcgacgg ccaggcggta
     3961 acgcgtttcg ctggaatgaa cgacagcgtg atgtggaatg tgatccggag tgaaggcgag
     4021 tcgattccgt ttgtcgtcga gcgggacggc cgccgcctct cgctctcgat cgtcccctac
     4081 aaggcgccga gcagcggctg gcggcgcaag agtgtccggc aggttttgat ttatcccgcg
     4141 accacgccgg tgatcgatca agtccaaccc aagacgccgg cagcggccgc cgggctgcag
     4201 cacggcgaca tcattctcgg ctacgatggc acgcggattt acagcccgat ctcgctcgcc
     4261 gcttacgtgg agacacacgc gggccagccg atcgcgctgg cggtcgagcg cggcggccag
     4321 gagctcaaga tcaacgtcac accggagctt ttgcctaacg aaaaaacgcc gcgcatcggc
     4381 atcgcctggg atacctcggg ccggatgagc atcgcgcatc cgaacccgat cgagcaggtt
     4441 tacaacagcg tgaccagcac cctggagacg atcggcgcgg tggcctcgcc gaagtccgac
     4501 gtgaaactgc agcatctcag cgggccgctc ggcatcgtgc gcatttatta cctgcttttt
     4561 gagagtgatc gcggctggca actggcgctt tggttcagcg tcatcctgaa cgtgaacctc
     4621 gcgattctga atatgctccc gattccagtg ctcgacggcg ggcacatttt gctcgcgttg
     4681 gtggaggccg tgcgacgcaa accggtcaac attcgcgtgc tcgaaatcgt ccagaccggc
     4741 tgcgccgtcg tcatcatcgg ctacatcgcc tacattagtt tcttcgatgt cggggattgg
     4801 ctgggcgaaa agcgcggctc ggatgacgca ccagccacgg cggtgcaggc cagcccgacc
     4861 ccgtagcgac gggccgcaga cattgcgcgg actttctgct gcgatatgtt ggtggcgcaa
     4921 tggacgtgaa ggaaattcgc gagctttacg aaacggcccc ctttcaatct tttgacctct
     4981 ttccgccgaa tggcagcaag gtccagattg atcatccgga attcatcgca ttctcccgcg
     5041 attatcggac gttgcacttt tacccccttg aaggcggtgc gcggcacatc gacgtcaagc
     5101 tgatcacaga agtgagagtg ccatcatcga atgggtcgaa gccgccgcga aagaagaaaa
     5161 ggtgacgctg gacttccccg gctcccccat ctcctttcag cgtcgccctt cgcgcgaagt
     5221 gatgg
//
LOCUS       DATMNS010000322         2300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4599305, whole genome shotgun sequence.
ACCESSION   DATMNS010000322 DATMNS010000000
VERSION     DATMNS010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 2300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2300
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4599305"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VNW28_11210"
     CDS             complement(<1..271)
                     /locus_tag="VNW28_11210"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10542.1"
                     /translation="MLLDEEKVRSLLRMEELIPAMAGALRDLSAGKVEQPVRLILPVA
                     EHNGFFGVMPAFGGGALGAKLVTFYPNNEGIPTHHAMILLFRPETG"
     gene            complement(276..1268)
                     /locus_tag="VNW28_11215"
     CDS             complement(276..1268)
                     /locus_tag="VNW28_11215"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXA10543.1"
                     /translation="METNKPMTPPPVRPITLTEIREAQARIAPTILRTPLVRLELGTE
                     FPDIRLKLENLQPINAYKLRGAANAVAMLPESERQRGVWTISAGNAGQGVAYAARQAG
                     VPCSVVVIETAPAAKVERMRALGAKLIPVPYEVAWQALDDRSYPGAEGTFIHPFDDDN
                     FIAGHGTMGLEILEDAPDTAAVIAGIGGGGLIVGLGSAFKALRPEIRIWGAEPETAAP
                     VALSFAAGSPQKFEDWKSSFVDGAGGQSVFPRMWDRMKPVVDGSFVVSLEATRRAMRL
                     MAEKVRVISEGAGALPLAAALTGKAGPGPIVCIVSGGNLDLKKFCELIDLQPND"
     gene            1303..1902
                     /locus_tag="VNW28_11220"
     CDS             1303..1902
                     /locus_tag="VNW28_11220"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXA10544.1"
                     /translation="MPRPLFLFAPGAGASASHPWMQRWRNRLQEIGEVEAFDYPYMRE
                     GRKRPDRLPQLIAAHREALAEARGKTGAGAPTILIGKSMGGRIGCHVALEEKVNGLVC
                     LGYPLCAMGDRSKLRDEVLRALETPILFVQGTRDPLCPLDLLESVRAAMKAPGILHLV
                     EGGDHSLGVGKRQLQAVGEAQEEVDRRILETIVRFVDAR"
     gene            1892..>2300
                     /locus_tag="VNW28_11225"
     CDS             1892..>2300
                     /locus_tag="VNW28_11225"
                     /inference="COORDINATES: protein motif:HMM:NF021988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HXA10545.1"
                     /translation="MRVEAYAPGRVELLGNHTDYNQGLVLAAAINRGLKVSGERRSDG
                     LITLRSSSLGEFEIARDDLRPQTEPRWANYVLGVVRELLDAGISVDGFSAAIEGDLPS
                     GCGLSSSAALEVATALFLLKLRTATLPPLEIARL"
BASE COUNT          413 a          717 c          736 g          434 t
ORIGIN      
        1 cgccggtttc cggtcggaag agcaggatca tggcgtgatg ggtggggatg ccctcattgt
       61 tagggtaaaa ggtcaccagc ttggccccga gcgcgcctcc gccgaaagcg ggcatcaccc
      121 cgaaaaaacc attgtgctcg gccactggca ggatgagccg caccggttgc tcgactttcc
      181 ccgcggaaag atcacgcaac gcgcccgcca tcgccgggat caattcttcc atccgcagca
      241 gcgagcggac tttttcttca tcgagcaaca tttcgttagt cgttaggctg gagatcgatc
      301 aattcgcaga actttttcaa atccaggttt cctccggaaa cgatgcagac aatcgggccc
      361 ggcccggctt ttccggtcaa ggcggcggcc agcggcagcg cgccggcgcc ctccgaaatg
      421 acgcgcactt tctcggccat caggcgcatc gctcttctcg ttgcttccag actaacgaca
      481 aacgaaccgt caaccactgg tttcattcgg tcccacatcc ggggaaaaac actctggccg
      541 cccgccccgt cgacaaacga actcttccag tcctcgaatt tctgcggaga tcccgcggcg
      601 aaggagagcg caacgggagc ggccgtctcc ggctcggcgc cccagatcct gatctccggc
      661 cgcagcgcct tgaacgcgct tccgagtccc acgataagtc cgccgccgcc gatgcctgcg
      721 atgacggccg cggtgtcggg cgcgtcctcc aggatctcaa ggcccatcgt gccgtggccg
      781 gcgatgaagt tgtcgtcgtc gaacgggtga atgaacgtcc cttccgcccc gggataagaa
      841 cggtcgtcga gggcctgcca cgcgacctcg tacgggaccg gtatcaattt cgcgccgagc
      901 gcgcgcatcc gctcgacctt cgccgccggc gccgtctcga tcacgacgac actgcaaggc
      961 acgccggctt gccgcgcggc gtaggccacg ccttggccgg cgttgcccgc gctgatcgtc
     1021 cagacaccgc gctgtcgttc cgactccggt aacatcgcga cggcattggc tgcgccgcgg
     1081 agtttgtagg cgttgatcgg ctggagattc tcgagtttca agcggatgtc ggggaattcg
     1141 gttcccagtt ccaggcggac gagcggtgtg cgcaagatgg tgggcgcaat gcgcgcttgc
     1201 gcttcccgga tttcggtcag ggtgatggga cggacaggcg gcggcgtcat gggcttgttg
     1261 gtttccattc cggatgcaga atgggaatca aactacgaag aaatgccaag gccgcttttc
     1321 cttttcgcac ccggtgccgg ggcgtcggcg tcgcatccct ggatgcaacg ctggaggaac
     1381 cgcctgcaag aaatcggcga ggtcgaggcg ttcgattatc cctacatgcg cgaaggtcgg
     1441 aagcggcccg atcgtttgcc acaactcatc gcggcgcatc gcgaagcgct ggccgaagcg
     1501 cgggggaaga ccggtgccgg cgcgccgacg atcctgatcg gaaaaagcat gggcgggcgg
     1561 atcggttgcc acgtcgcttt ggaggagaag gtgaacggcc tcgtttgtct cggctatccg
     1621 ctctgcgcga tgggcgatcg gagcaagctg cgcgacgaag tgttacgcgc gttggagacg
     1681 ccgattctgt ttgtgcaggg aacgcgcgac ccgctttgtc cgctggatct tctcgaaagc
     1741 gtgcgcgccg ccatgaaagc ccccggcata ttgcatctcg tggaaggcgg cgatcattcg
     1801 ttaggcgtag gcaaacgcca actgcaggca gttggggaag cgcaggagga ggtcgatcgc
     1861 cggatcctcg aaacgatcgt tcggttcgtc gatgcgcgtt gaagcctacg cgccgggccg
     1921 ggtggagctg ctgggcaatc acaccgacta caaccagggc ctggtcctgg ccgcggcgat
     1981 caatcgcggg ctgaaagtga gcggcgaacg acgcagcgac ggactcatca cactccgatc
     2041 ttcatcgtta ggcgaattcg aaatcgcgcg cgacgacctc cggccgcaaa cggaaccgcg
     2101 ctgggcgaat tacgtgctcg gggtcgtcag ggagttgctt gacgctggta tttcggttga
     2161 cggattttcg gccgcgatcg agggcgacct gccgtccggt tgtggtcttt ccagttccgc
     2221 cgcgctcgaa gtcgcgacgg ctctctttct gcttaagctc cgcacggcga ccctgccacc
     2281 gctcgagatc gccaggctct
//
LOCUS       DATMNS010000323         6132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4275141, whole genome shotgun sequence.
ACCESSION   DATMNS010000323 DATMNS010000000
VERSION     DATMNS010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 6132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6132
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4275141"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(102..2477)
                     /locus_tag="VNW28_11230"
     CDS             complement(102..2477)
                     /locus_tag="VNW28_11230"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HXA10546.1"
                     /translation="MIFRRLAFLLVALCWVSSAGAQLTAGDVTKIISQAASRAQQISP
                     NSVIAVVDREGFVLGVWNVRGEPTPSPQEVATCVSKAGSAAFLSSDQNAFTSRTAGFI
                     IQQHFPPGVFNTPTGPLVGVGLSSLFISDINKFKAPGSVTIFSATPGTAIVKVPFTSL
                     DGTPGGVPLYKGGQLVGGVGVTGDGTPPAIVGFRAENPFVFIAGYDKDEDVALAGQIG
                     YKPSSDILATNVFINGISLEYTESSTQLPSTLTLPGNVAAAYPIQDSPPPFPYPVVTL
                     GGVQGQIRQPIINDPIAGTINGQARLTAAEVTGILAAAADRVRTTRAGIRLPIGVQME
                     AFISVVNFPNQNNVTPTVLGTFRTGEATVFSWDVAVQKARTALAFSNNGNTVAMSSRT
                     VGFLAQSHYPPGIDANHAGPYFGMQEAVSGLLGTLSNVTLNPDFVVNPNLPNGITIFP
                     GGFPLYRNGQMIGAIGVSGDGVDQDDLVAASGTVNFLTPLAIGADEVVFEGARLPYAK
                     FPRDPSGTLGSNDPVILAAPTAVTVTQGLANISTRMNVGPGENQAIAGFIISGTASKD
                     LLVRALGPSLAQFGVSGVLANPALEIHNQSGTVIASNDDWQSTQKDAITASGFAPSND
                     AEAAVRLTLPPGAYTAVVSGGTSGGGIALAEVYDLSPTSASKLANISTRGFVGQEGSV
                     MIGGFILGGETGCPKVVVRALGPSLTSLGVSGALADPTLELHDSNGALVTSNDNWADT
                     QALDLQAAALAPANPLESATLGWLTPGAYTAIVRGNGLQTGVGLIEVYCLQ"
     gene            2658..4730
                     /locus_tag="VNW28_11235"
     CDS             2658..4730
                     /locus_tag="VNW28_11235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10547.1"
                     /translation="MNVGRFLLCLLVFSAPARAQEEASELPPEDTSDSLRLRDLGTQR
                     FTLAQIQRLAPEAAREAAAPSPSPSPPTQFNPDPGAPNEPESIEPPSLFPPVIPPLPQ
                     YGEEALPRSLELPRHDMREVVPNRHNLEYENYPDAAQNQGLLPDSQAVPNRWFVGFGR
                     WKRYADPSTETPYQSGGLKLWHPYLPSTLKGDAPIIGEDIFLDLTLSDFFQFEARRLP
                     TPSGVSSARPNSSEFFGRSEQYFLSNDVSIGIDLFQGETAFKPVQWALRFLGVYNNNY
                     TDVQETNVLDPDPRLANTTRYKDFYALQEAFAEIHIRDLSNNYDFISSRFGIQPFVSD
                     FRGFIFNDSNLGVRIFGNYDNNRWQYNLAAFDMLEKDTYSDLNTFDSRDQQVLIANVF
                     RQDFFAKGYTAELSFLADLDGASRHYDKNGFITRPAPIGTVQDHSLEAYYLGWTGDGH
                     IGWLNITHAFYEVLGEDNLNGIAGRRVSINAQMAALELSIDRDWLRQKITLFYASGDS
                     KPRDGTATGFDTIIDRPFLFGGPFSFYVHQGFNLAGTAVNFKQGDSLVPDFRTSKTEG
                     QANFVNPGVFLVGYGLDADVTPKLKAFVNLNYIQTMTTAVTEQVLFTNRASNDIGFDF
                     SLGVQWRPLLTDNVIISAGMGFLVPGQGYKDIYETNTRPVPGFGNPPAGSVDDFLYSG
                     IFTVTLTY"
     gene            4730..5185
                     /locus_tag="VNW28_11240"
     CDS             4730..5185
                     /locus_tag="VNW28_11240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HXA10548.1"
                     /translation="MKTQQKTFNVQRRAEAARVREDPSADFGQTGDTFAKYDLEDRLL
                     EFTARLLKLVDALPGSRAGNHIAGQLLRCGTSPYANHGEVQAAESRKDFIHKLGVCFK
                     ELKEIKRWLRLIARVELIPVKRVRLLLLETEELLRIFSASIRTAENNAR"
     gene            5224..>6132
                     /locus_tag="VNW28_11245"
     CDS             5224..>6132
                     /locus_tag="VNW28_11245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10549.1"
                     /translation="MNVERFLLPLLLFLPSFGSAQDSTSGYGPPTVVSPNESMTAPAA
                     LPPRNWIDQTQAEADAKSIGCNECHQGVEPMHAAPHVVLGCTDCHGGNPARHLKIEQA
                     HVLPRYPEFFRTSANPPRSDVWLNHESPEFIRFMNPGDLRVAEQTCGLCHGEIVHKVA
                     HSMMNHGAMLWGAALYNNGGVPYKNYRFGQAYGADGVPLRLLNYTPVTPNDTKVHGIL
                     PFIEPLPRFNLSNPGNVLRIFEKGGEKQLQLGVPTSDEPPGRPARRLSERGLGTLNRT
                     DPVFLGLQKTRLHDPLLGFLGTNDHPG"
BASE COUNT         1211 a         1931 c         1791 g         1199 t
ORIGIN      
        1 tgacttcgga tcagaaggtt gggggttcga atccctccag gcacggcttc tcaaatagtt
       61 ggctcggtca aaaggacgaa ttgtcgcttc aggatgccga attattgcag gcaatagact
      121 tcaatcagcc caacacccgt ctgcaagcca ttgccgcgca cgatggcagt gtaggcccct
      181 ggcgtgagcc agccgagggt ggcggactcc agcgggttgg ccggggccag ggctgctgct
      241 tgcaggtcga gggcctgcgt gtcggcccaa ttgtcgttcg aggtcaccag cgcgccattc
      301 gagtcgtgca actccagcgt cggatcggct agcgcgccgc tgacaccgag ggatgtcagc
      361 gaaggaccaa gcgctcgcac caccactttc gggcagccgg tttcgccgcc gagaataaat
      421 ccgccgatca tgacggaacc ctcctggccg acaaatcccc gcgtgctgat gttggcgagc
      481 ttggaagcag aggtcgggct caggtcgtag acctcggcca gagcgatccc gccgccactc
      541 gttccacctg aaacaacggc ggtgtaggcg cccggcggga gagtcagcct aacggcggct
      601 tcggcatcgt tcgacggcgc gaagccgctc gcagtgatcg cgtctttttg cgtgctttgc
      661 cagtcgtcgt tcgacgcgat gacggtgccg ctttggttgt gaatttccag ggcgggattc
      721 gcgagcacgc ccgaaacgcc gaattgggca agcgacggac cgagcgcccg caccagcaga
      781 tccttcgatg ccgtgcccga gatgataaag ccggcgatcg cctggttctc gcccggaccg
      841 acattcatcc gggtggaaat gttcgccagc ccctgggtga ccgtaaccgc cgtgggcgcg
      901 gcgaggatga ccgggtcgtt gctgcctaac gttcccgagg gatcgcgcgg aaatttcgca
      961 taaggcaggc gcgccccctc gaagacgacc tcatccgccc cgatcgcgag cggggtgagg
     1021 aaattgaccg tgccagaagc ggccacgaga tcgtcctggt cgacgccgtc gccgctcacg
     1081 ccgatcgcgc cgatcatctg tccgttgcga tagagcggga agccgccggg gaaaatcgtg
     1141 atgccgttag gcagattggg attgacgacg aagtccggat tgagagtgac gttcgagagc
     1201 gtgcctaaca aacccgagac cgcttcctgc atgccgaaat acgggccggc gtgattcgcg
     1261 tcgatccccg gcgggtagtg gctttgcgcg aggaaaccaa ccgtgcgcga cgacatcgcc
     1321 accgtgttgc cgttattcga aaaggcgagc gcggtccgcg ctttctggac cgccacatcc
     1381 caactgaaaa cagtcgcttc gccggttcga aaagttccga ggacagtcgg agtgacgttg
     1441 ttttggttcg gaaaattcac cacgctgatg aacgcctcca tctgaacacc gatcggcaat
     1501 cggattccgg cgcgcgtcgt gcggacgcga tccgcggcgg ccgcgagaat gccggtcact
     1561 tccgccgccg tgaggcgagc ctggccattg atcgtacccg cgatcgggtc gttaataatc
     1621 ggctggcgaa tctgtccctg cacgccgcca agggtgacca ccggataagg aaagggcggc
     1681 ggcgagtcct ggattggata cgcggcggcg acatttcccg gcagggtcag cgtgctcggc
     1741 agttgcgtgc tactctcggt gtactcgaga ctgatgccgt tgatgaaaac gtttgtcgcc
     1801 agaatgtccg acgacggctt gtaaccgatc tgcccggcca gggcgacgtc ttcgtccttg
     1861 tcgtagccgg caataaaaac gaatggattc tccgctcgga aacccacgat cgcgggcggc
     1921 gtgccgtcgc ccgtgacacc gacgccgccg acgagctgtc cgcccttgta aagcggaacg
     1981 ccgcccggcg ttccatccag cgacgtgaag ggcaccttta caatggcggt gcccggcgtc
     2041 gcactgaaga tcgtgacact gcccggtgcc ttaaatttgt tgatgtcgga gataaagaga
     2101 ctcgacagac cgaccccgac aagcggcccg gtcggggtgt tgaaaacgcc gggcgggaaa
     2161 tgttgttgga tgataaatcc ggccgtgcgc gaagtgaacg cgttctgatc gctgctcagg
     2221 aaggcggccg agcccgcctt ggaaacgcag gttgcgactt cctgcgggga cggggtcggt
     2281 tctccccgga cattccaaac gccgagcacg aagccctcgc gatcaaccac cgcgatcacg
     2341 ctgttcgggg aaatttgttg tgcccgcgaa gcggcctggc tgatgatttt tgtcacatcg
     2401 cccgccgtta gttgcgcgcc cgctgacgag acccagcaaa gggctacgag aaggaaggcg
     2461 agcctgcgga aaatcatccg actggctttg tagcaaccga tggcacgaac tgccaagaaa
     2521 aaacccccgg tgcgacaggc ttgcaaatcg atcgccaatt ccgtcattct gcgcgggcga
     2581 tgcgcaaacc tgccgcggcg acgaggagtc acgcaatgat ttgcctgcct cttccttgga
     2641 acgttgaacg ttggacgttg aacgttgggc gttttcttct ctgcctgctg gttttctctg
     2701 cgcccgcgcg agcgcaggaa gaagcgtccg aacttccacc ggaagacacg agcgattccc
     2761 tccgcctgcg cgatctcggt acccagagat tcacgctcgc ccagatccag cggctcgcgc
     2821 cggaggcggc cagggaagct gccgcgccct ctccttcgcc ttcgccaccg acgcagttca
     2881 atcccgaccc gggcgcccct aacgaaccgg aatcgatcga gccgccgagt ttgtttcccc
     2941 cggtcatccc gcccctcccg cagtacggcg aagaagcgct cccgcgctcg ctcgagctgc
     3001 cgcgtcacga catgcgcgaa gtcgtgccga accggcacaa cctcgagtac gagaattatc
     3061 ccgacgccgc gcaaaaccag ggtctcctgc ccgattccca ggcggtcccg aaccgctggt
     3121 tcgtcggctt cggacgctgg aaacgctatg ccgatccttc gacggagacg ccctaccaat
     3181 caggcggtct gaaactctgg catccctatt tgccgagcac gctaaagggc gacgcaccga
     3241 tcatcggcga ggatattttt cttgatctca cactgagcga tttcttccaa tttgaagcgc
     3301 gccgcctgcc gactcccagc ggcgtgagca gcgcgcggcc gaacagctcg gaattcttcg
     3361 gccgcagcga gcagtatttt ctctcgaacg atgtctcgat cgggatcgat ctctttcagg
     3421 gcgagaccgc cttcaaaccg gtccaatggg cgctccgttt tctcggcgtt tacaacaaca
     3481 attacaccga cgtgcaggag accaacgtgc tcgaccccga tccgcgcctc gcgaacacga
     3541 cgcgctacaa ggatttctac gcgctccagg aagccttcgc cgagattcac atccgcgatc
     3601 tttcgaataa ctacgacttc atttcctcgc gctttggcat tcaaccgttc gtgagcgact
     3661 tccgcggctt cattttcaat gactccaacc tcggcgtgcg catcttcggc aactacgaca
     3721 acaatcgctg gcaatacaat ctcgccgcct tcgacatgct cgaaaaggat acctactcgg
     3781 atttgaacac tttcgattcg cgcgatcagc aggtcttgat tgcgaatgtt tttcgccagg
     3841 atttcttcgc caagggctac accgcggagt tgagtttcct cgccgatctt gacggcgcga
     3901 gccggcacta cgacaagaac ggtttcatca cccggccggc gccgatcggc acagtgcagg
     3961 atcattccct ggaggcgtat tacctcgggt ggacgggcga cggccacatc ggctggctca
     4021 acatcacgca cgctttttat gaggtcctcg gggaggacaa tctcaacgga attgcgggcc
     4081 ggcgggtctc gatcaatgcg cagatggcgg cgcttgaact ctcgatcgac cgggactggc
     4141 tccgccaaaa gatcacgctc ttttacgcga gcggcgacag caagccgagg gatgggaccg
     4201 ccaccggttt cgacacgatt attgaccggc cttttctttt tggcggacca ttcagttttt
     4261 acgtgcacca gggcttcaat ctcgcgggca cggcggtgaa ttttaagcag ggcgacagcc
     4321 tggtgcccga tttccgcacc agcaagaccg agggtcaggc caacttcgtc aaccccggcg
     4381 tgttcctcgt cggttacggc ctcgatgccg atgtcacgcc gaaactgaag gctttcgtca
     4441 atctgaacta catccagacg atgaccaccg cggttaccga acaggtgctc ttcaccaatc
     4501 gtgcgagcaa cgatattggg ttcgatttca gcctcggagt tcaatggcgc ccgctgctca
     4561 ccgacaacgt gatcattagc gcggggatgg gttttctcgt cccggggcag ggctacaagg
     4621 atatctacga gactaatacg cggccggtgc cggggtttgg gaatccgccg gcggggagtg
     4681 tggatgattt tctgtatagc gggattttta ccgtcacgct gacctattga tgaagacgca
     4741 gcagaaaacg ttcaacgttc aacgaagagc agaggctgcg cgcgtccgtg aagatccaag
     4801 tgcagatttc ggtcaaactg gcgacacctt tgcgaagtac gatttggaag atcgcttgct
     4861 cgaattcacg gcgcgacttc tcaagcttgt cgacgcactg ccaggttcgc gcgccggaaa
     4921 ccatatcgcg ggtcaattgc tgcgttgtgg aacttcgccg tatgcaaacc acggcgaagt
     4981 ccaagcggcg gaatcccgca aggactttat ccacaagctt ggcgtttgct ttaaggaact
     5041 aaaggaaatc aaacgctggc tccgcctcat tgcgcgagtc gagctcattc ccgtcaagcg
     5101 agtgcgtctg ctccttctgg agacggaaga actactgagg atcttctccg cgagcattcg
     5161 cacggcggag aacaacgcgc gatgactggt tcttctcttc ccttgaacgt tgaacgttgg
     5221 acgttgaacg ttgaacgttt tcttcttcct ctcctacttt tccttccttc cttcggcagc
     5281 gcgcaggatt ccacctccgg ctacggtcct cccaccgtcg tctcccctaa cgaatcaatg
     5341 actgctcccg ccgcgctccc tccgcgcaac tggatcgacc aaacccaggc ggaggccgac
     5401 gccaagagca tcggctgcaa cgaatgtcat caaggcgtcg agccgatgca cgccgccccg
     5461 cacgtcgtgc tcggctgcac cgattgccac ggcggcaacc ccgcccgcca cctgaaaatt
     5521 gagcaggcgc acgtcctgcc gcgttatcct gagttctttc gcacctcggc caatcccccg
     5581 cgctccgatg tctggctcaa ccacgagtcg ccggaattca ttcgtttcat gaaccccggc
     5641 gacctgcgcg tcgcggagca gacctgcggc ctttgtcacg gtgagatcgt gcacaaggtc
     5701 gcgcacagca tgatgaatca cggcgccatg ctctgggggg ccgcgcttta taataacggc
     5761 ggtgtccctt acaagaatta ccgcttcggc caggcctatg gcgcggatgg cgtgccgttg
     5821 cgcctcctca attacactcc ggtcacgccg aacgacacca aggtgcacgg cattctcccg
     5881 ttcatcgagc cgctcccgcg cttcaacctg agcaacccgg gcaacgttct gcgcatcttc
     5941 gaaaaaggcg gcgagaaaca actgcagctc ggcgtcccca cgagcgacga gccgcccggc
     6001 cggccggcgc gccggctatc cgagcgcggt ctcggcacgc tcaatcgcac cgatccggtc
     6061 ttcctcggcc tgcaaaaaac gcggctgcac gacccgcttc tcggcttcct cgggacgaac
     6121 gatcacccgg gc
//
LOCUS       DATMNS010000324        14805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4281083, whole genome shotgun sequence.
ACCESSION   DATMNS010000324 DATMNS010000000
VERSION     DATMNS010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 14805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14805
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4281083"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /gene="lptB"
                     /locus_tag="VNW28_11250"
     CDS             <1..662
                     /gene="lptB"
                     /locus_tag="VNW28_11250"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXA10550.1"
                     /translation="IKLEVGQGEIVGLLGKNGAGKTTSFYMIVGLVRPNSGRVIFSGT
                     DVTNYPMYRRARLGMGYLPQEESIFRKMTVEQNILAILETMPLSKKERRHRCDELLHQ
                     FGIERIAKNTALTLSGGEKRRLTIARSLVTRPKLLMLDEPFSGVDPIAVYDVQQLVVN
                     LRKSGLAILITDHNVRETLSIVDRAYLIDEGRVVSEGTKDFLINDPISRQLYLGERFR
                     M"
     gene            677..1252
                     /gene="raiA"
                     /locus_tag="VNW28_11255"
     CDS             677..1252
                     /gene="raiA"
                     /locus_tag="VNW28_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HXA10551.1"
                     /translation="MQTVNAHPNVIVTGRHLAITDAINDYATRKIEGLHLDYPKIIEA
                     HVILGVEKYRHWAEVVLICSNHITIEACEETSDLYAAIDAVMDKIARQMRKYKTRLLR
                     RHRPRKEEIRHLEEQILNPEPIEEDEHHEHAVVRTERYPVKPMFVDEAVLQLEMSARQ
                     FMVFMNARNSRVNVLYRRKSGDFGLIEPTLG"
     gene            1597..3258
                     /locus_tag="VNW28_11260"
     CDS             1597..3258
                     /locus_tag="VNW28_11260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HXA10552.1"
                     /translation="MKKPTLIQHLRSNPGAAIAASITLLFSLGVASASAQSQTLVTRH
                     TRDAVSKGQVAFVQHLDAGQTLHLVIGLPLRNQENLNSFLQNLYNPYSPNYRQYLSVE
                     QFTKMFGPTQEDYEAVIQFAQRNGFVVSGTASNRLIVEVDASVASIEKAFNVTMNVYQ
                     HPTEKRTFYAPDREPTVNLSAKLWNINGLDNFAIPRPASLKQNLTDQPASNTGSGPGG
                     YFLGSDLRKAYYGSTGTLTGTGQVIGLLEYVGYNPADVTKYFTTYGPPLTTTVTPVST
                     DGTAAICTTCDDTEQALDIEYSISMAPGLSQCIVYVGSTDQSMLNRMATDNTAKAISC
                     SWSWKPADPTVDDPIFMQMAAQGQTFASASGDSSSWGAGEYNWPQESANILCVGANHL
                     VTNGPGGSWLSETGWSDSGGGISIDRIKIPSWQLNKKVVNATNGASKKLRNGPDVAAE
                     GDFQNYICYDGTCAGGWGGTSFACPEIIGYLGLANQQSVSHGHATLGFVNPAIYTIGE
                     GAAYTSNFHDITSGSNVGFTCVPGYDLVTGWGSPNGATLINTLAP"
     gene            complement(3448..4320)
                     /locus_tag="VNW28_11265"
     CDS             complement(3448..4320)
                     /locus_tag="VNW28_11265"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXA10553.1"
                     /translation="MRPRVLVAGCGYLGVATADLLHAGGWEVEGWTQSPESARRLAAK
                     PYPVRAVDIAELAAVKAAASACEAVIHCASSGGGGPESYRRIYFVGAENLLAALAPRI
                     FVYTSSTSVYAQTGGEWVDEESAAEPLHETGRILRATEELVRQKGGLVARLAGIYGPG
                     RSALLRKFLSGEARLEAGEERYLNQAHRDDIAAALFLLVHLPNEERTTTGIVNVADNE
                     PMTQRACYAWLAARLHRPLPALVDPPAGRKRGASNKRVSNQRLRALGWQPRFPTFPTG
                     MENSVLPASSTLGA"
     gene            complement(4317..5042)
                     /locus_tag="VNW28_11270"
     CDS             complement(4317..5042)
                     /locus_tag="VNW28_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HXA10554.1"
                     /translation="METEYKVRLEIFEGPLDLLLYLIKRDELDIYDISLERITQQYLE
                     YLQAFKELNIDVAGEFVVMAANLIYLKSRNLLPADQQPPEEDVDEDDPRWELIRQLIE
                     YKKFKEAAAHLQSRELAQERIFARIGEGVVPEIAPLPLGEVGIFQLINAFQNVIKRLQ
                     AQDDLREMFGEHFTVSDKIERILQSLAGGKSMRFSDLFAQMASRVEIVVTFLALLELI
                     RLKQVRALQPNEFDEIEIAAVAT"
     gene            complement(5045..5833)
                     /gene="mazG"
                     /locus_tag="VNW28_11275"
     CDS             complement(5045..5833)
                     /gene="mazG"
                     /locus_tag="VNW28_11275"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HXA10555.1"
                     /translation="MDASTNFRRLCEIVAQLRAPGGCPWDREQTHQSLVPGLLEEAYE
                     VAAAIRTGDDANFREELGDLLLQAVMHAQIASEEGRFTIEDVAREIGDKLVRRHPHVF
                     AGSEARDTGAVLRQWDAIKRAEKSAGEAPYFAGLTRALPALMFAQKAQTKAARVNFDW
                     QEIADVVAKVDEELGETKEAIASQNADAMADELGDLLFAVVNLARKSQLDAETVLAAA
                     TEKFISRFHALERELQAQGKQLGAVDLAALDEIWNRVKTEPDLA"
     gene            complement(5873..6586)
                     /locus_tag="VNW28_11280"
     CDS             complement(5873..6586)
                     /locus_tag="VNW28_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HXA10556.1"
                     /translation="MANETHGPIDEATLAAHGSFYDQGYVNAMSHFYRGELGRIMVWR
                     TRLDTTTNWAVTSSTAIITIAFSNAAVPHLIFFFNLAIVWIMLWIEARRYRFYDAFRA
                     RVRMLEAHFLVPMVMENRNLLQGEWKKLVCEDLILPSFKIGKLEAVGRRLKRNYVFIF
                     VLIMIAWVMKIFIHAKEPIDGIPAFYRALRVGHIPSWLVAFIFVGTLVGVIGITIYVG
                     RKTSGEVSEFGSHRSLWRI"
     gene            6648..7106
                     /locus_tag="VNW28_11285"
     CDS             6648..7106
                     /locus_tag="VNW28_11285"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HXA10557.1"
                     /translation="MISPLLDALLDPKHVALELRGASAGEAVWEIVELLRESGALPKP
                     KEFFEAVMEREQKSSTVANGGVAFPHARTQLVEQILLGIGRSRKGVLFGEGKEPVHLI
                     FVIAVPQQMVNDYLICVGALARLVRQEGTRQALLAASTPAEFFDRMRNEP"
     gene            complement(7062..7829)
                     /locus_tag="VNW28_11290"
     CDS             complement(7062..7829)
                     /locus_tag="VNW28_11290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04283 family arsenosugar biosynthesis
                     glycosyltransferase"
                     /protein_id="HXA10558.1"
                     /translation="MSDRATAALRLSVIIPAWNDHANLARLLPVLVRLNAFHEVIVAD
                     ASPQPAAAESIRTAGGIYLPATQPNRGAQMNLAAETATGDVLIFHHADSILTLAHVAA
                     IGSALRDPAVIGGAFYRKFDGRHPQFLWFERVARFFTRHGGSFFGDQTLYVRRETFRA
                     LGGFAPIPLMEDMEFSRRLRRAGKVAVLDPPVESSTRRHLRRGPWRTSIENGLFIVLY
                     KCGLSPVRLHRWYYGERGPVPNDSRLVAHPVEEFGRR"
     gene            complement(7826..8713)
                     /locus_tag="VNW28_11295"
     CDS             complement(7826..8713)
                     /locus_tag="VNW28_11295"
                     /inference="COORDINATES: protein motif:HMM:NF042638.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6576 domain-containing protein"
                     /protein_id="HXA10559.1"
                     /translation="MLALSEPAPFTIGMFGVVKSDDYQPVGWMGRYPIRITTLIAALF
                     VLGMFGTVIAESAGWDLTPFAFRSSAFLHGALWQPLTCILIQQASFFFIFNILFLYWS
                     GVEVEKFLGRRRYLHLIGLLLLVPPLVMMLWSAAGVRWTYAGSYEISVGLFIAFATLY
                     PGVELFGWVTLKWLAFAGLVLASMQYLPAHQWGFLTVLWGMCLTSFLYIRLVQKPEAS
                     ALQSAFSEGVSRLFRRKQKFRVLPHETGRRTIEPEDDVVESIDPLLDKISKSGIGSLT
                     ASERRALDRARNRLLKKSP"
     gene            8913..10685
                     /locus_tag="VNW28_11300"
     CDS             8913..10685
                     /locus_tag="VNW28_11300"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HXA10560.1"
                     /translation="MGENKPANQAVLDWVHEVELLTQPENVFWCDGSEAENDYLLGEA
                     CRQGVLLKLNQEKNPGCYLHRSNPNDVARVEQFTLICAPTKEEAGPTNNWAEPATMYR
                     KLHGMLKGALRGRTMYVVPYIMGPPESPLAKIGFEITDSIYVVLSMRIMTRMGALAGA
                     RLGAKPDAEFNRGVHSLLDVNPERRFICHFPQDNTIISVGSGYGGNVLLSKKCLALRI
                     ASYLAKKQGWLAEHMLILGVESPDGRKHYVAAAFPSACGKTNFAMLIPPPHFAGWKIT
                     TIGDDIAWMQIGRDGRLYAVNPENGYFGVVPGTSYKSNANAMRSIERDTLFTNVALTS
                     DGDVWWEGKDGDPPAEAIDWRGRPWTPASKEKAAHPNSRFAVPMRNNPVLDPEVDDPA
                     GVPISALIFGGRRSDTMPLVFQAADWNHGVYLGATMASETTAAATGAVGQVRRDPMAM
                     LPFCGYNIGDYFRHWLKTGPRLTNPPLIFHVNWFRKSAAGKFLWPGFGDNMRVLRWII
                     DRCAGRGGAGESPIGSVPRPEDLDLAGLEDVSPEMMRALLAVKPDEWARELAGQAEFF
                     ASLQPYVPKELLDEHKKLAGRLGR"
     gene            complement(10758..12158)
                     /locus_tag="VNW28_11305"
     CDS             complement(10758..12158)
                     /locus_tag="VNW28_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HXA10561.1"
                     /translation="MVTQRTQGLYRVFLLCQIALVAALFWFGVWVMVTFYSAGAELTW
                     RRYSIYCAMLCIGMLGESLSREGSKKYFLQSELLHQHRLALRQTFASVGVLVLYLIAT
                     KDSFISRLFFFNFLPWLYIALLFSHHFLPAFLARGMFRREEKTLLVGSSAKAAQLRGW
                     LRRKAELGLRTVGLISEEPLEYTADRIPVLGGLECFRSAIQEHEVTQVILLEFPQFDE
                     RSRNLIATCDQLGVRLLIVSDLEEKLRHPVTHFDDDGFRFIGLREEPLENPFNRGLKR
                     TIDYALALPIMLFIFPPLAVLVWLAQRLQSPGPLFHKQVRAGIQNRRFEIWKFRTMHP
                     ENPDLARQATSEDERVYPLGKWFRKLSIDEIPQFWNVLHGDMSVVGPRPHLLEHNEQF
                     SQLMANYQVRTFVKPGITGLAQVRGFRGEARNSKDIENRVACDIEYLENWNMTLECGI
                     IARTIAQLFVPPPSAY"
     gene            12298..14673
                     /locus_tag="VNW28_11310"
     CDS             12298..14673
                     /locus_tag="VNW28_11310"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXA10562.1"
                     /translation="MQPAESPRSPRSSTSNAAAWTALGLPPLVVLAAFLGGATQRWSE
                     AVVVGAFALLLLARPPRFSLGPFLNTAALLFLALAAVAFLPARWFFMPAWRVALTNDF
                     GVQLASTVSPQPWLTLDGLLLLIAGLAWIYYVATLDAHLRDVRQAARAFSGGIIALAG
                     LCIYLHLTDRALPFWHNERSFGPFPNRNQTGDLFGISTLLVLACLQDDFRRGHRRWII
                     WLAGVGVLIAALILDFSRAGILILVLGSVAWLARLALHRWSGARIAVATSLLLVLFAG
                     LLIFGGETIERFPLRLGSEGAVTSDFRWLIFRDAATMIQSSPWCGVGLGNFESVFALF
                     RDVSRGETRSLHPESDWLWVAAEMGWPALVLIFAAAAVFVKRVFPLREGTNQRLRYAA
                     LVGALLFALHGFVDVSGHRFATFLAGTLLLGLAQFRPPPTAASRWPPILFRFVGLLLV
                     VVSVAWLFSWRRALPVPGFTGVENAKEDAIVANRGHRFDEAIAFADCGLEWAPLDWQL
                     YFIRATARIGARQPPARALEDFRRARFLEPSAYQLPYEEGKAWLGAQPILTLTAWREA
                     LQRQGAESARIYSRMLGDAADFDPAVLEGLRQYALDYPALTINYLESVHGARFEFALH
                     EVLARDPGLKQFSSAQKTRLFALWSGEESRAGLLQAVETHPEWLEFAWPGIARYHAGR
                     QEFEAAWQLVRQHAPEPSLPQAGNNESMPQLERKLYASANDYAAGYALFRAQMAAGKI
                     DDALATARHCTGQAEAPPYFLFLQAEAWAATKNWERAWQSWEEYESAVLPK"
BASE COUNT         2968 a         4524 c         4489 g         2824 t
ORIGIN      
        1 ggatcaaact ggaagtcggc cagggcgaga tcgtcggctt gctcgggaaa aacggcgccg
       61 ggaagacgac cagcttctac atgatcgtcg gtctcgtcag gccaaacagc ggccgggtta
      121 tcttctccgg caccgacgtg accaattacc cgatgtaccg gcgcgcccgg ctcgggatgg
      181 gctatctgcc gcaggaggaa tcgatctttc gcaagatgac ggtagagcaa aacatcctcg
      241 ccattctcga gacgatgccc ctgagcaaaa aagagcgccg ccatcgctgc gatgaattgc
      301 tccatcaatt cgggatcgag cggatcgcga aaaacaccgc gctcaccctg agcggcggtg
      361 agaagcgccg cctcacgatc gcgcgctccc tcgtcacccg gccaaaatta ctcatgctcg
      421 acgagccctt cagcggcgtc gatccgatcg cggtctacga cgtgcagcaa ctcgtcgtca
      481 acttgcgcaa atccggcctc gccattctca ttaccgacca caacgtccgc gagacgctct
      541 ccatcgtgga ccgcgcctac ctcatcgacg aaggccgcgt ggtgagcgaa gggacgaaag
      601 attttctgat taacgatccg atcagccgcc agctttatct cggcgagcgc tttcggatgt
      661 gaaccaaagg agccgcatgc aaaccgtcaa cgcccatccg aacgtcatcg tcaccggtcg
      721 ccatctcgcg atcaccgacg cgatcaacga ttacgccacg cgcaagatcg aagggctgca
      781 cctcgattat ccgaaaatca tcgaggccca cgtcattctc ggcgtggaaa aataccggca
      841 ctgggccgag gtcgtactta tttgctcgaa ccacatcacg atcgaggcct gcgaggaaac
      901 gagcgacctc tacgcggcga tcgacgcggt aatggacaaa atcgcgcggc agatgcgcaa
      961 gtacaaaacg cgccttttgc gccggcatcg cccccgcaag gaagagatcc ggcatctcga
     1021 agaacagatt ctgaaccccg agccgatcga ggaggacgaa catcacgagc acgccgtcgt
     1081 ccggacagag cgttatccgg tgaaaccgat gtttgtcgat gaggcggtgc tccagttgga
     1141 gatgtcggcg cgacagttca tggtcttcat gaacgcgcgg aactcgcggg tcaacgtttt
     1201 gtatcgccgc aagagcggcg acttcgggct gatcgagcca acgttaggct aagagtggag
     1261 gcgggcatgt cgcccgccaa cgcggttaat cccggccccc gacacggacg ccgccaaatc
     1321 ctgggcgcaa cgccgggtgc gtcgcaggcc ggcaggaccg ccgctccgtc ccatcgcccc
     1381 gccagagggg ccctgcgacc tgaaggaaca aagatgacat tttaatgaca aaaatgtcag
     1441 aattggccct cgcggtccta gggcacggag tcgtttgcca ttcgggccag gcgctcaaca
     1501 tgacattttt gtaatatttt tctggacatc ttccgctctc acctgtgtaa tcgcgtctcc
     1561 ggaaataccg ggaaactaaa accaaaaata ctaaaaatga aaaaacctac gctaattcaa
     1621 catctcagga gcaaccccgg agcagcaatc gctgcttcga tcacgctcct tttttccctt
     1681 ggagtcgcga gcgcttccgc gcagtcgcag actttagtga cgcgccacac gcgcgacgct
     1741 gtttccaagg ggcaggttgc cttcgtgcaa cacctcgatg ccggacagac cctgcatctc
     1801 gtgattggcc tgccgttgcg aaatcaggag aatctaaatt cgtttctcca aaatctctat
     1861 aatccctaca gccctaacta tcgccagtat ctaagtgtcg agcaattcac caaaatgttt
     1921 ggaccgacac aagaagacta cgaggccgtg attcagtttg cgcaaaggaa tggcttcgtg
     1981 gtctcgggaa ccgcttctaa ccggttgatt gttgaagtag atgcttccgt ggcaagcatt
     2041 gagaaggcgt tcaacgtcac gatgaacgtt taccaacatc caaccgaaaa gcggaccttc
     2101 tatgcaccgg atcgcgagcc aacggttaac ctgtcggcca agctatggaa tatcaatggg
     2161 ctggataatt ttgcgatccc gcgcccggcc agtctgaaac agaacctaac tgaccaaccc
     2221 gcaagcaaca ctgggtcggg ccccggaggc tatttccttg ggagtgacct gcgcaaggct
     2281 tactacggat ccaccggtac gcttactggc accggtcaag tcatcggatt gctggaatat
     2341 gtgggttata accctgccga tgtcaccaaa tacttcacca cctatggccc gcctctgacc
     2401 acaaccgtga caccggtttc aaccgacgga acggcggcga tttgtacgac ctgtgacgat
     2461 accgaacaag cgcttgatat cgagtattcc atttcgatgg cacctggctt gagccagtgc
     2521 attgtctatg tcggaagcac cgaccaatcg atgctcaacc ggatggcgac cgacaacacc
     2581 gccaaagcaa tcagctgctc gtggtcatgg aagccggccg atccgacggt tgacgatccg
     2641 atcttcatgc agatggccgc gcaggggcag acatttgcca gcgcttcagg agacagctcg
     2701 agctggggcg cgggcgaata caactggccg caggagagcg cgaacatcct ctgcgtcgga
     2761 gcaaatcatc tcgtgaccaa cggtccgggt ggcagctggc tgtctgaaac tggctggagc
     2821 gatagcggcg gaggtatttc catcgaccgc attaagattc cgagctggca gctaaataag
     2881 aaagtcgtga acgccacgaa cggcgcttcg aagaagcttc gtaacggacc ggacgtagcg
     2941 gcggaaggcg acttccaaaa ctacatttgc tatgacggca cctgcgccgg cggctggggc
     3001 ggcacaagct ttgcctgtcc cgaaattatt ggttaccttg gtcttgctaa ccaacagtcg
     3061 gtttcgcatg gtcatgccac attaggcttc gtcaatccgg cgatttatac catcggcgaa
     3121 ggcgcagctt atacgagcaa cttccatgac attaccagcg gcagcaatgt tggcttcacc
     3181 tgtgtgcccg gctacgacct cgtgaccggt tggggtagtc cgaatggagc gaccttgatc
     3241 aataccctgg cgccctaaag aggcgacagc ttcagattga tcagtttaac gacccaggag
     3301 tgcatctgga caacagatgc ctcctgggtc ctctttttcg gggaacggcg gaactcgcgg
     3361 gtcaacgtcc tctaccggcg caagggcggc gacttcggac tgatcgagcc aacgttaggc
     3421 aaagaaccgc ggctggccgc gctgcggtca ggcaccgagg gtcgaagacg cgggcagaac
     3481 ggagttctcc atgccggtcg ggaaggtggg aaaccggggc tgccagccga gcgcgcgcag
     3541 gcgctggttc gacacgcgtt tgttgctcgc gccgcgtttt cgcccggcgg gtgggtcgac
     3601 gagggcgggg agcggacgat gcaacctggc cgcgagccat gcgtaacacg cccgttgcgt
     3661 catcggctcg ttgtccgcca cgttcacgat ccctgtggtt gttcgctctt cgttaggcag
     3721 atggacgaga agaaagagcg ccgcggcgat atcgtcgcgg tgcgcctgat tgagataacg
     3781 ctcttcgccg gcctcgaggc gcgcctcgcc ggagaggaat ttgcggagca gcgcggagcg
     3841 gcccggtcca taaattcccg cgagccgcgc gaccagcccg cccttttgcc taacgagttc
     3901 ttcggtcgcg cgcaggatcc ttccggtttc gtgcaacggt tccgccgcgc tctcttcatc
     3961 gacccattcg ccgccagtct gggcgtaaac cgaggtgctg ctggtgtaaa cgaaaatgcg
     4021 cggcgcgagt gcggcgagta gattttcggc gccgacgaaa taaatccggc gataactttc
     4081 cggcccacca ccgccggagc tggcgcaatg aatcacggcc tcgcacgcgc tcgccgccgc
     4141 cttgaccgcg gcgagctctg cgatgtcgac ggcgcggaca ggatacggct tggccgccag
     4201 ccgccgcgcg gactcgggcg actgcgtcca gccttccact tcccatcccc cggcgtgcaa
     4261 gagatcggcc gtcgccacgc cgagatagcc gcagccggca actaaaacac gcggcctcac
     4321 gtcgcgaccg ccgcgatctc gatttcgtca aattcgttag gctgaagggc ccgcacctgt
     4381 tttagacgga tcagctcgag caaggcgaga aacgtaacca cgatttcaac ccgcgacgcc
     4441 atctgcgcga acaaatccga aaagcgcatc gacttgccgc cggcgaggct ttgcagaatc
     4501 cgctcgatct tgtcggagac ggtaaaatgc tcgccgaaca tttcgcgcaa atcgtcctgc
     4561 gcctgaagcc gcttgataac gttttgaaag gcgttgatca gctggaaaat tcccacttcg
     4621 ccgagaggca aaggcgcgat ttccggcacg acgccttcgc cgatgcgcgc aaaaatccgc
     4681 tcctgcgcca gttcgcgcga ttgcaagtgc gccgcggctt ccttgaattt tttgtattcg
     4741 atcaactggc ggatcaattc ccagcgcggg tcatcctcat cgacatcctc ctccggcggc
     4801 tgttggtcgg ccggcagcaa gttccggctc ttgaggtaaa tcaggttggc ggccatgacc
     4861 acgaactcgc cggcgacatc gatgttcagc tccttgaagg cctgaagata ttccaggtat
     4921 tgctgggtaa ttcgctcgag cgaaatgtcg tagatatcga gctcgtcgcg cttgatcagg
     4981 taaagcagga gatcgagcgg gccttcgaaa atctccagcc ggactttgta ttcggtctcc
     5041 atcgttaggc aagatcgggc tccgtcttca cccggttcca aatttcgtcg agggccgcga
     5101 gatcgacggc gccgagctgc ttgccttgcg cttgcagctc gcgctccaac gcgtgaaaac
     5161 gtgaaatgaa tttctccgtc gccgcggcca gcacggtctc ggcatcgagc tgacttttgc
     5221 gtgcgagatt tacgaccgcg aaaaggagat cgcccaactc atccgccatc gcgtcggcgt
     5281 tttgcgaagc gattgcttcc ttcgtttcgc ccagttcttc gtcgaccttc gcgacgacgt
     5341 ccgcgatctc ctgccaatcg aagttcactc gcgccgcttt ggtctgcgct ttctgggcaa
     5401 acatgagggc cggcagagct ctcgttaggc cggcaaaata cggcgcttcc cccgcgcttt
     5461 tttccgcgcg cttgatcgcg tcccattgcc ggaggacggc gccggtgtcg cgcgcttcgc
     5521 tcccggcgaa gacatgcggg tgccggcgga cgagcttgtc gccaatctcg cgggccacat
     5581 cctcgatcgt aaagcgtccc tcttcgctcg cgatctgcgc gtgcatcacc gcctgcaaaa
     5641 gcagatcgcc gagttcttca cgaaagttcg cgtcgtcgcc ggtgcggatg gcggccgcca
     5701 cttcgtaagc ttcctccagc aaacccggga cgagtgactg gtgggtctgc tcacgatccc
     5761 acgggcaacc gcccggcgcg cgcaattgcg cgacgatctc gcagaggcga cgaaaattgg
     5821 ttgaggcgtc cacgcgtttc cttccaccat caaccgatcg ccggcaaccg cttcaaatcc
     5881 gccagagcga acgatggctc ccgaactcgg agacttcgcc ggaggttttg cgcccgacat
     5941 aaatcgtaat gccgatcacg ccgaccagcg tgccgacaaa aatgaaagcc accagccagg
     6001 acgggatgtg accgacgcgc agcgcgcggt agaacgccgg aatgccatcg atcggttctt
     6061 ttgcgtggat aaaaattttc atcacccacg cgatcatgat gagaacgaaa atgaaaacgt
     6121 aattgcgttt caggcgtcgg ccgaccgctt cgagttttcc gattttgaac gagggcagga
     6181 tcaaatcttc gcagaccagt tttttccatt cgccttgcag caggttgcga ttctccatca
     6241 ccatcgggac gaggaaatgc gcctcgagca tgcgcacccg cgcgcggaag gcatcgtaaa
     6301 agcgataacg gcgcgcctcg atccagagca tgatccagac gatcgcgaga ttgaaaaaga
     6361 agatgaggtg cgggacggcg gcattggaaa aggcgatcgt gataatcgcg gtgctgctcg
     6421 ttaccgccca gttggtcgtc gtatcaagcc gcgtccgcca gaccatgatg cgccccagct
     6481 cgccgcggta aaaatgcgac atcgcattga cgtagccctg gtcataaaag ctgccgtggg
     6541 cggcgagggt cgcttcgtcg atcggtccgt gagtttcgtt cgccacgatt cggttgcatg
     6601 attcgacttc tgcgcggggc gcgtcaatct atcaatttac cgatccgatg atctcaccct
     6661 tgctcgacgc cttgctcgat ccgaaacacg tcgctctcga actgcgcggc gcgagcgcgg
     6721 gagaggcggt ctgggaaatc gtggagctgt tgcgcgagag cggcgcactg cccaagccga
     6781 aggagttttt cgaagcggtc atggagcgcg aacaaaaaag ctccacggtg gcgaacggcg
     6841 gggtcgcttt cccacatgcg cgcacccagc tggtcgagca aattctcctc gggatcggcc
     6901 gcagccggaa gggggtcttg tttggcgaag ggaaagaacc agtgcacctc attttcgtca
     6961 ttgccgtgcc gcagcagatg gtgaacgact atttgatctg cgtcggcgcg ctggcccggc
     7021 tggttcgcca ggaggggacg cggcaggcgc ttctggcggc ctcaacgccg gccgaattct
     7081 tcgaccggat gcgcaacgag ccttgagtcg ttaggcacgg gcccgcgctc gccataatac
     7141 caacgatgca ggcggacggg cgaaagcccg cacttgtaga ggacgatgaa aagcccgttc
     7201 tcgattgagg tgcgccacgg gccccggcgc aaatgtcgcc gggtggagga ttccaccggc
     7261 ggatcgagca cggccacttt gcccgcgcgg cgcagccgtc gagaaaactc catgtcctcc
     7321 atcagcggga tgggcgcgaa gccgcccagc gcacgaaagg tttcgcgccg cacgtagagg
     7381 gtttgatcgc cgaaaaaact gccgccgtgg cgggtaaaaa agcgggccac cctctcgaac
     7441 cagagaaact ggggatggcg accgtcgaac ttgcggtaga aggcaccgcc gataacggcc
     7501 ggatcccgca gggccgaccc aatggccgcg acgtgggcga gggtcaggat cgaatcggcg
     7561 tgatgaaaaa tcagcacatc gccagtggcg gtttccgcgg cgagattcat ctgcgcgccg
     7621 cggtttggtt gcgtcgccgg cagataaata cccccggctg tccggatgga ctctgcggcc
     7681 gcgggttggg gggaggcgtc cgccacaatc acttcgtgga acgcatttag cctaacgaga
     7741 acaggcagga gtcgggcgag attggcgtga tcgttccagg ccgggatgat cacgctgagc
     7801 cgcaacgcag cggtcgcccg atcgctcacg gcgacttctt caagagccgg ttgcgcgccc
     7861 ggtccagcgc gcgccgctcg ctcgccgtca ggctgccgat gccggatttc gagattttat
     7921 cgagcagtgg gtcgatcgat tcgacgacgt catcctccgg ctcgatggtt cggcggccgg
     7981 tctcgtgcgg caggacacgg aacttctgtt ttcgccgaaa gaggcgcgat actccttcag
     8041 agaatgcgct ctgcaaggca gatgcttcag gcttctggac gagccggatg tagaggaagg
     8101 aagtgaggca cattccccaa agcaccgtga ggaaacccca ttggtgagcg ggaaggtatt
     8161 gcatcgaggc aagaacgaga cccgcgaagg cgagccattt cagagtcacc caaccaaaca
     8221 gctcgacccc ggggtaaagc gtcgcgaagg cgatgaacag accgaccgag atctcgtacg
     8281 agcccgcgta ggtccagcgg accccggctg cgctccagag catcatgacc aatggaggca
     8341 cgagcagcaa aagcccgatg aggtgcagat agcgccggcg accaagaaat ttttcgactt
     8401 caacccccga ccagtagagg aagagaatgt tgaagataaa aaagaacgaa gcctgttgaa
     8461 tcagaataca ggtcagtggt tgccagagcg cgccgtgcag aaaggcagag gagcgaaagg
     8521 cgaatggcgt cagatcccag ccggcggatt ccgcgatgac cgtgccgaac attcccagca
     8581 cgaaaagggc ggcgatcagc gtggtgatcc ggatcgggta acgacccatc caacccaccg
     8641 gttggtagtc atccgatttc accacaccga acatgccgat agtaaacggc gcaggctcgg
     8701 agagcgcaag cacgcttgcg tatggcaacg gccgtgtcgg ctgcacggat gctacccccc
     8761 tggcaatgtc tgtgggtccc cacggctgcc gctccagcac cgtgggattt ttttcttggc
     8821 ctaccggctg aagcgttcaa actaacactg cactcgcggt cgcgggccag actatggaaa
     8881 atatcgctcc tgccatttcg ccgcccagtg gcatgggtga aaacaaaccg gccaaccagg
     8941 cggtgctcga ttgggtgcac gaggtcgaac ttctcacgca gccggagaac gttttctggt
     9001 gcgatggatc ggaggcggag aacgattacc tgctggggga agcctgccgc cagggggtcc
     9061 tgctgaagct taaccaggag aagaaccccg gttgctatct gcatcgttcc aaccccaacg
     9121 atgttgcccg agtcgaacaa ttcactctca tctgcgcgcc gacgaaggaa gaagccggtc
     9181 cgacgaacaa ctgggcggaa cccgcgacca tgtaccgcaa actgcacggc atgctgaagg
     9241 gcgcgttgcg tggccggacg atgtacgtcg tgccctacat catgggaccg ccggagtcgc
     9301 ccttggccaa gatcggtttt gaaatcaccg actcgatcta cgtcgtcctc agcatgcgga
     9361 tcatgacgcg catgggagcg ctggccggtg cgcgcctcgg ggcgaagccg gatgcggaat
     9421 tcaatcgcgg cgtgcattcg ctcctcgacg tcaatccgga gcggcgcttc atctgccatt
     9481 ttccacagga taacacgatc atctccgtcg gttccggtta cggcgggaac gttctcctga
     9541 gcaaaaaatg cctcgccttg cggattgcct cctacctcgc gaaaaaacag ggctggctcg
     9601 ccgagcacat gctgatcctg ggggtcgaat cgccggacgg gagaaaacat tacgtcgcgg
     9661 cggccttccc aagcgcgtgc gggaagacga atttcgcaat gctcattcca ccgcctcatt
     9721 ttgccggctg gaaaatcacc actatcggcg acgacatcgc gtggatgcag atcgggcggg
     9781 acggccggct ctacgcggtc aatccggaga acggctattt cggtgtcgtg cccggcacga
     9841 gctacaaatc gaatgcgaac gcgatgcgtt ccatcgagcg ggacacgctc tttacgaacg
     9901 tcgccctgac gagtgatggc gatgtctggt gggaaggaaa ggatggtgac ccgccggcgg
     9961 aggcgatcga ctggcgcggc cggccctgga cgccggcgtc gaaggaaaag gcggcccatc
    10021 ccaatagccg tttcgccgtt ccgatgcgca ataatccggt gctcgatcct gaagttgacg
    10081 acccggccgg ggtcccgatc agcgcgctca ttttcggcgg gcgccggagc gacacgatgc
    10141 cgctggtttt tcaggccgcc gattggaacc acggggttta tctcggagcg acgatggcct
    10201 ccgagacgac ggcggctgcc accggcgcgg tcggccaggt gcggcgggat ccgatggcga
    10261 tgctgccttt ttgcggctac aacatcggcg attatttccg gcactggctc aagacggggc
    10321 cgcgcctcac gaatccgccg ctgattttcc acgtcaactg gttccgcaaa agtgcggccg
    10381 gaaaatttct ctggcccggc tttggcgaca acatgcgagt tttgcgatgg atcatcgatc
    10441 gctgcgccgg ccgcggcgga gcgggggaaa gcccgatcgg cagcgtgccg cgcccggagg
    10501 atctcgatct cgccgggctg gaagacgtct cgccggagat gatgcgcgcg ctcctggcgg
    10561 taaagccgga cgaatgggcg agggagctgg cggggcaggc cgagttcttt gcctcgctcc
    10621 agccttacgt gccgaaagag ctgctcgacg agcacaagaa actggccggg cgacttggtc
    10681 gttaggcaga ttcggctcct ccgactccgc tgcgctcggc tcgggctgac cggacggaac
    10741 cgtcgggcaa cctgccctca ataggccgag ggcggcggca cgaagagctg ggcgatggtg
    10801 cgcgcgatga tcccgcactc cagcgtcatg ttccagtttt cgagatactc gatgtcacag
    10861 gcgacgcgat tctcgatgtc tttgctgttt cgcgcttcgc cccgaaaacc gcgcacctgc
    10921 gcgagaccgg tgattcccgg cttgacgaac gtgcgcacct gatagttcgc catcagttgc
    10981 gaaaactgtt cgttatgctc gagcaagtgc ggccgcgggc cgacgacgct catgtcgcca
    11041 tggagcacat tccaaaactg cgggatctcg tcgatgctca gtttgcgaaa ccatttgcct
    11101 aacggataga cacgctcgtc ttcggaagtc gcctgccgcg cgaggtccgg attctccggg
    11161 tgcatggtgc ggaatttcca aatctcgaag cgccggtttt gaatcccggc gcgaacttgc
    11221 ttgtggaaga gcggcccagg cgattgcagc cgctgcgcga gccagacgag caccgcgaga
    11281 ggcggaaaaa tgaacagcat gatggggagc gcgagcgcgt aatcgatcgt ccgtttgagt
    11341 ccgcgattaa acggattctc gagcggctcc tcccgcaggc cgatgaagcg aaaaccatcg
    11401 tcatcgaaat gggttaccgg atggcgcagt ttttcctcca ggtcgcttac gatcaaaagc
    11461 cggacgccga gttggtcgca ggtcgcgatg aggttgcggc tccgttcgtc gaactgcggg
    11521 aactccagca ggatcacctg cgttacttca tgttcctgga tcgcgcttcg gaaacactcc
    11581 aggcctccga gcacgggaat cctgtccgcg gtgtattcca gcggttcctc cgagatgagg
    11641 cccaccgtcc gcaaacccag ctcggccttg cgccgcagcc agccgcgcaa ttgcgcggct
    11701 ttggcactcg agccgacgag caacgtcttc tcttcccgac gaaacattcc gcgggcgaga
    11761 aacgcgggta gaaagtgatg cgaaaagagg agcgcgatgt agagccaggg aaggaagtta
    11821 aagaagaaaa gtcgcgaaat gaaagagtcc ttggtcgcga tgagataaag gacgagcacc
    11881 ccgacactgg cgaaggtctg gcgcagggcg aggcggtgct ggtggaggag ctcgctctgg
    11941 agaaaatatt ttttcgagcc ctcccggctg agggattccc ccagcatccc gatgcagagc
    12001 atcgcgcaat agatactgta gcggcgccag gtgagctccg cgccggccga gtaaaacgtc
    12061 accatcaccc agacgccgaa ccagaagagc gccgccacga gcgcgatctg gcaaagcaga
    12121 aaaacgcgat acaatccttg cgttcgctgg gtgaccatgg ttatcgactg gaggcaaaga
    12181 cggcggcggg cgcggttggg gaatcgatca gtcgaccccc gtgaaagata ctgattcgct
    12241 gcatttgaga gcgcaagctc gttacgccaa ttccacttca tcgcaactag acgatcgatg
    12301 caacccgccg agtccccgcg atcccccaga tcgagcacga gcaacgccgc cgcctggacg
    12361 gcgctcggct tgccgccgct cgttgtgctg gccgcgtttc tgggcggcgc cacgcaacgc
    12421 tggagcgagg cggtcgtcgt cggcgctttc gcgctcctgc tcctcgcccg gccgccgcgc
    12481 ttttcgctcg ggccgttcct aaacacggcg gctctgcttt tcctcgcgct ggcggcggtc
    12541 gcctttcttc cggcgcgctg gtttttcatg cccgcctggc gcgtcgccct aacgaatgac
    12601 tttggggtgc aactcgcttc caccgtcagc ccgcagccct ggttaacgct cgatggcctg
    12661 ctcctcttga tcgccgggct ggcctggatt tactatgtcg cgacgctcga cgctcatctc
    12721 cgcgatgtgc gacaagcggc ccgcgctttc tccggaggaa tcatcgcgct ggccgggctt
    12781 tgcatttatc tgcatctcac ggatcgcgcc ctgcccttct ggcataacga acggagcttc
    12841 gggccattcc ccaatcgcaa ccagaccggc gacctctttg gcatttccac cctcctcgtg
    12901 ctcgcctgcc tgcaggatga tttccggcgc ggtcaccgac gctggattat ctggctcgct
    12961 ggagttggcg tcctgatcgc cgcgctcatt ctcgattttt cgcgcgccgg catactcatt
    13021 ctcgtgctcg gctcggtggc ctggctggcg cggctcgcgc tccacaggtg gtcgggcgcc
    13081 cgaatcgcag tcgcgacctc gcttcttctg gtgctcttcg ccgggctgct cattttcggt
    13141 ggcgaaacga tcgagcgttt tcccctgcgc ctcgggagcg aaggcgcggt cacctccgat
    13201 tttcgctggc tgatatttcg cgacgccgcg acgatgatcc agtcgtcacc ctggtgcggt
    13261 gtgggactcg gaaatttcga gagtgtgttt gcgctctttc gcgatgtctc ccgcggtgaa
    13321 acccgctcgc tccatccgga aagcgactgg ctctgggtcg cggcggaaat gggttggccg
    13381 gcgctcgtcc tcattttcgc ggccgccgcc gtcttcgtga agcgcgtctt tcccttgcgg
    13441 gaagggacca accagcggct gcgctacgcc gcgctcgtcg gtgcgctcct tttcgcgctg
    13501 cacggattcg tcgatgtctc cggtcaccgt ttcgcgactt tcctcgcggg gacactgctc
    13561 ctcggtctcg ctcagtttcg cccgccgccg acggcagcga gtcgttggcc gccgatcctc
    13621 tttcgtttcg tcggtctgct gctcgtggtc gtaagtgtcg cctggctttt ttcctggcgg
    13681 cgcgccttgc ccgtgcctgg attcaccggg gtggagaacg cgaaggagga cgccatcgtg
    13741 gccaatcgcg gccaccgttt cgatgaggcg atcgccttcg cggattgcgg tctggaatgg
    13801 gcgccgctcg attggcagct ctacttcatc cgggccactg cgcgcatcgg cgcgcgccag
    13861 ccgccggcgc gggcgctcga ggatttccgg cgggcccgtt tcctcgagcc gagcgcctac
    13921 cagttgccct acgaggaagg gaaagcgtgg ctcggcgcgc agccgattct cacgctgacg
    13981 gcctggcgcg aggcgttgca acggcagggc gcggaatcag cccggatcta ttcccggatg
    14041 ctgggtgatg ccgcggactt cgatcccgcg gtgctcgaag ggctgcgcca gtatgccctc
    14101 gattatccgg ccctaacgat caattacctg gagtccgtcc acggagcgcg tttcgaattc
    14161 gcgctgcacg aagtgctggc tcgcgacccc ggcctcaagc aattcagctc cgcccaaaag
    14221 acgcggcttt tcgcgctttg gtcgggcgag gaatcgcgcg ctgggctttt gcaggcagtc
    14281 gagacccatc ccgagtggct ggagtttgcc tggccaggga tcgcccgata tcatgccggc
    14341 cgccaggagt tcgaagcagc atggcaactc gtcaggcagc atgccccgga gccctcgctc
    14401 cctcaggcgg gtaataacga gtccatgccg cagctcgagc ggaaactgta tgccagcgcg
    14461 aacgattacg ccgccggtta cgccctcttc cgggcgcaga tggccgcggg caagatcgac
    14521 gacgcgctcg ccacggcgcg gcactgcacc gggcaggcgg aggccccgcc ctatttcctt
    14581 ttcctccagg ccgaagcctg ggcggccacg aaaaattggg agcgcgcctg gcaatcctgg
    14641 gaggaatacg agtcagcggt gttgcccaaa tgactgccgc gataccgaat cggcataacg
    14701 aaaagtcgat cggaatgcag cggcaatcgt cgggactgtg cgcgctgatg tgatggaatt
    14761 atttgcctgc tttctcgacg ggtcgacgtg cccgccatga ccgac
//
LOCUS       DATMNS010000325        13286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4637358, whole genome shotgun sequence.
ACCESSION   DATMNS010000325 DATMNS010000000
VERSION     DATMNS010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 13286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13286
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4637358"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..2050)
                     /gene="tkt"
                     /locus_tag="VNW28_11315"
     CDS             complement(59..2050)
                     /gene="tkt"
                     /locus_tag="VNW28_11315"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXA10563.1"
                     /translation="MSLNLAVLSRAADQARGLCIDAVHASQSGHLGLPLGCAEMGAVL
                     YGYALKYNPDQPRWINRDYLVLSAGHGSMFLYAWLHLSGFDLPMAEIKRFRQLHSKTP
                     GHPEFMETPGVECTTGPLGQGVANAAGIAMACKMAAARFNTDEHRIFDQHVVCLAGDG
                     CLQEGVSAEASAFAGHFGLDNLILIYDSNAVTLDAMAKETQSEDTGMRFRAYGFDVQE
                     IDGQDMQQFLDAFNVAKERDNGKPQMIIAHTLIGKGVPEVAGTSKAHGEGGAKFAEAA
                     RKGLGLPEEHYFVSDDVRAYFTQHKQDLQEAYDRWEKTYRDWRKKNADKAQLLDDAIA
                     RKVPADLFEKIPNFPSDAKLATRKAGSEVLQPLAQALPFLISGSADLHGSTLNYIKDG
                     GDFTRATPAGRNIHFGIREHGMCAIMNGVSYHGLFRASGATFLVFTDYCRASLRLAAL
                     SHLPNIYIFTHDSIAVGEDGPTHEPVETVSSVRLMPQIDVIRPADPEETAGAFVAAAQ
                     RTDGPTLLALTRQVVPLLNDIDAKLRREGVLRGGYIAQKETAKLDLILLSCGSELQHA
                     LAAAKELGAGTRVVSMPCFERFARQPESYRAEVLPNECRKRVAIEAGVPEIWSQYVGL
                     DGKIVALHRFGMSAPGNEVMKELGIDAQHVIAAARSLGN"
     gene            complement(2217..3455)
                     /locus_tag="VNW28_11320"
     CDS             complement(2217..3455)
                     /locus_tag="VNW28_11320"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HXA10564.1"
                     /translation="MRNRLTMFLLLGGLALSGCKKDSLAGKVKPVTPSPAPAVAVVAT
                     PTPPAEPRKPLIDQNAQVVVFGYHRFEEKVRRPDTEITPAAFTAQMQELKDKKIAVIS
                     MQDFLAWKRGEKAIPSHCAVITIDDGWKTGYDVAWPILKKFNYPFTLFIYTEGIKGGK
                     YGGGAAMSWEQLAEMRDAGIDIEAHSATHQDLRKPYDKSTKRKLNPQEYNEWLESEVG
                     GSKATLEQKLGIKVNCFAVPFGYYNDRVKEETKKANFEAVFTVYGQKLTYGSPNDSLG
                     RYMIEGNKPKVFEDAINFGGSSGGGSAPVEELPITSISPQPADGSTATSRPLIKANLG
                     AIGGIDPGSVRMRVSGLGVVPANYDAANKILSYQVTQPLHGESCFVIVEAKIGGKKAE
                     AHWSFSLKEGAVIASPTPKK"
     gene            3581..4282
                     /locus_tag="VNW28_11325"
     CDS             3581..4282
                     /locus_tag="VNW28_11325"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXA10565.1"
                     /translation="MPRSFVSLAALLLAATFLLGGCATVATNTSHTFDTVVVDAGHGG
                     KDSGAYRRNAPPEKIVALDVALRVDRKLRDAHLKTVLTRSNDTFIPLDSRVALGNAQE
                     NSIFVSIHFNDSRRRGVEGVETYYHSPYAYDLAAAIERNLSTLPGTVNRGVRTAGFRV
                     LRNAHYPSVLVECGYLSNRSEARDAADADYREMLADRIAQAIVDYRYGGGTYRRPAIA
                     QTSAGGGASAVPSFR"
     gene            complement(4432..5664)
                     /locus_tag="VNW28_11330"
     CDS             complement(4432..5664)
                     /locus_tag="VNW28_11330"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXA10566.1"
                     /translation="MEAIQIYRSLNATQRRTFLACFLGWTLDALDFFLLTFVVRNVSA
                     EFSVSIVKVTFAITLTLMMRPLGALIFGLLGDRFGRRIPLMVDIIFYSLMELLTAFSP
                     NYTVFLIFRALYGIGMGGEWGLGASLAMESLPARARGLASGILQQGYSFGFLLAAVVY
                     WLVFPHFGWRALFVVGALPAVLVIYIRAHVPESAVWQRQHATATEKSLGGWRMLIENW
                     RLLIYAVLLMTAFNYMSHGTQDLYPTFLQKQRGLSVNQTAPIAIIYNLGAICGGTVLG
                     FYSQRWGRRRTIIIAAVFGILLIPAWIFSSTLPLLIAGGFFMQFMVQGAWGVVPVHLN
                     ELSPPAWRGTFPGVVYQLGNFFSANAALLQAKLAEHFVRPNGQPDYALTMACVTLAVF
                     VVLILLAALGREARGIEF"
     gene            5901..10460
                     /locus_tag="VNW28_11335"
     CDS             5901..10460
                     /locus_tag="VNW28_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HXA10567.1"
                     /translation="MHTASTHPLAALLRQRIVILDGAMGTMVQRHKLSEEQYRGARFA
                     AHPAKHLKGDLELLQLTQPEVIETIHLQYLEAGADIIETNTFSATTIGQHDFLFAGEP
                     ESGRKDAAFFERVVADSELAALVSEMNLAAARVARAVADRVAHQTGRPRFVAGSLGPL
                     PVTASLSPDVNDPSFRAVTFDQIRRAYGEQARALLEGGVDLLLLETIFDTLNAKAALF
                     AIAEVFESFGKKLPVLISGTITDLSGRTLTGQTVGAFLTSILHAEPLVVGLNCALGPA
                     EMRPHVEEMARLAPCFTSAYPNAGLPDPLSPTGFPETPESLAPQLREWAKNGWLNIVG
                     GCCGTTPEHIRALAEAVRDCPPRVLPLWSARAPSRGGGISGASRETSSHEVSGLNVDS
                     SSYAVEEVRGEAPRTAREARALPGPEAPLHLAGLEALDVTPDFGFVVVGERTNITGSP
                     KFSKLILAGDFEGALVVARHQVEGGANILDVNLDEGMIDSEAAMTRFLHLIGSEPDIS
                     RLPIMIDSSKWSVLEAGLKCVQGKGVVNSISLKAGEEEFLRQARLVRRYGAAVIVMAF
                     DERGQADTFARKTEICTRAYRLLTEAAGFPPNDIIFDPNILTIATGLEEHNEYAIAFV
                     EATRWIKQNLPGARVSGGVSNISFSFRGNNTVREAMHSAFLYHAIRAGLDMAIVNAGQ
                     LAVYEEIDPPLRELVEDVLFNRRPDATERLVDFAEKVKSQGKVAPDEEIWRSAPVEER
                     LAHALVKGIVEHIEADTEEARQKSARPLDVIEGPLMAGMSVVGDLFGSGKMFLPQVVK
                     SARVMKKAVAYLMPYMEAEKQAGAKPQGRIVMATVKGDVHDIGKNIVGVVLQCNNYEV
                     IDLGVMVPAEKIFETARARQANIIGLSGLITPSLDEMVHVAQEMERQQFRLPLLIGGA
                     TTSRAHTAVKIAQHYHESVVHVLDASRAVGVVSALLNPDAKLKFDATTRADYERLRTE
                     HANRTREKKMLTLAQARTNRLTIDWSSYEPPAPGFLGVRVVGTPGSARAPRAVSGASP
                     ETSREGDIHYSKRRLPHFERPWSKYAVVFSTHERRPLHPSERDLVLQSILTAHERNQI
                     ELYAACVMPDHVHLLFEPQIKQSAADGSAVFWPLTDILRGIKSSTAHRINKAAGRTGP
                     VWERESFDRLIRSESDLQEKFDYIMKNPWTSEIVRRDEDYPWLCTPKTSRRGAEKSTR
                     GACPPQTTRNSQATSVVEISLETLVEYIDWSPFFHTWELRGRYPAIFDDPVVGPQARE
                     LFADAQKLLDRIVAEKLLTARGVYGFWPANSLGDDVEIYRDELRSEVLARFYFLRQQM
                     QKPAGQSNYCLADFVAPKGSGRRDYLGGFAVTAGLGADDLAKHFQGELDDYNAILTKA
                     LADRLAEAFAEYLHREARLAWGFGAEEGLTTDDLIRERYRGIRPAPGYPASPDHVEKR
                     TLFDLLGAEDSAGITLTESFAMHPGASVSGLYFSHPAARYFAVGKIERDQATDYAARK
                     GESLGEIEKWLSPNLNYEPKREAAR"
     gene            complement(10667..11563)
                     /locus_tag="VNW28_11340"
     CDS             complement(10667..11563)
                     /locus_tag="VNW28_11340"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HXA10568.1"
                     /translation="MFKNEKINIAIFTILVLLQLLGVAAVWTRFHPFYLPIMAVLYLW
                     IGLGTTLYLHRYLTHRGFEMPGWLKFIFATGSAIGLSGDPVTWVGDHRFHHLKSDTEE
                     DIHSPRHGFPYAHMMWLIRKPASFRERSLRYAADVRRIWYCKLWENSALYVLPHLAVA
                     SLLFATVGLAGMLWCLYVPILIIYNCTWAVNSICHMKQFGYRSFETSDESKNNFWVGL
                     GALGEGYHNNHHAKPRCAAHGLKWWEFDFTRYTIWMLEKLHLAWNVVWPEPKAAEKEV
                     EEISADEAGTMLITSADPNSVL"
     gene            11842..11915
                     /locus_tag="VNW28_11345"
     tRNA            11842..11915
                     /locus_tag="VNW28_11345"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11876..11878,aa:Pro,seq:tgg)
     gene            12119..12436
                     /locus_tag="VNW28_11350"
     CDS             12119..12436
                     /locus_tag="VNW28_11350"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXA10569.1"
                     /translation="MFSVYVLKSGATGRRYVGSCANLSDRLRRHNAGESLATKHGMPG
                     TLIHAEQFPTRVAAAARERYFKTGQGRDELDHLQRAERSPRRQVAASCIVTPTPEFPG
                     DER"
     gene            12625..13035
                     /locus_tag="VNW28_11355"
     CDS             12625..13035
                     /locus_tag="VNW28_11355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXA10570.1"
                     /translation="MPAAADLQMLNALFKQTSLGKAADDYRLHVQVVRLESQIRNDAE
                     LQAMRASAEQTSTDLEKRKRLKAYYHQYYSRLRTMADTPDLKVYLDGQEAAHAGLLAQ
                     PRVRPNTNPVAASATATPLPTPVQARAGAVQRHP"
     gene            13122..13196
                     /locus_tag="VNW28_11360"
     tRNA            13122..13196
                     /locus_tag="VNW28_11360"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13155..13157,aa:Glu,seq:ctc)
BASE COUNT         2624 a         4170 c         3997 g         2495 t
ORIGIN      
        1 ggccgggtcc ttagtcctcg aatagatctg gagcacaggc gagaagcctg tgctccatct
       61 aattacctaa cgatcgtgcg gccgcgatca cgtgctgggc atcgatcccg agttccttca
      121 tcacttcatt accgggtgca ctcatcccga accgatgcag cgcgacgatc ttcccatcca
      181 gcccgacgta ctgcgaccag atctccggca cccccgcctc gatcgcgacc cgtttgcggc
      241 attcgttagg caagacttcc gcgcgatagc tttccggttg ccgcgcgaag cgctcgaagc
      301 acggcatcga gacgacgcgc gtccccgcgc ccagctcttt cgcggccgcg agcgcgtgct
      361 gcagctcgct cccgcacgag agcaggatca ggtcgagttt cgcggtttcc ttctgcgcga
      421 tgtagccgcc gcgaagcacg ccttcgcgtc gcagcttcgc gtcgatatcg ttcaagagcg
      481 gtacgacctg ccgcgttaac gcaagcaggg ttgggccgtc ggtccgttgc gccgcggcga
      541 cgaaggcgcc ggccgtttct tccggatcgg cgggccgaat gacgtcgatc tgcggcatca
      601 gccgcacgct gcttaccgtc tcgactggct cgtgcgtcgg accgtcctcc ccgaccgcga
      661 tcgagtcgtg cgtgaagatg taaatgttcg gcaaatgcga gagcgcggcg aggcggagcg
      721 aggcgcggca atagtccgta aaaaccaaaa acgtcgcgcc cgaagcgcgg aagaggccgt
      781 ggtaactgac gccgttcatg atcgcgcaca tcccgtgctc gcggatgccg aaatggatgt
      841 tgcgcccggc cggggtggcc cgcgtgaaat cgccgccgtc cttgatgtag ttgagggtgg
      901 agccgtgcag atcggcgctc ccgctgatca gaaagggcag cgcctgcgcg agcggctgga
      961 gcacttcgct cccggctttg cgggtggcga gcttggcgtc gctaggaaaa ttcggaatct
     1021 tctcgaataa atcggccggg actttccgag caatcgcgtc gtcgagcagc tgcgccttgt
     1081 ccgcattctt cttccgccaa tcgcggtacg ttttctccca gcgatcgtag gcctcttgca
     1141 ggtcttgctt gtgctgcgtg aagtatgcgc gcacgtcgtc ggaaacgaag taatgctcct
     1201 ccggcaggcc gagacctttg cgcgcggcct cggcaaattt ggcgccgcct tcgccatgcg
     1261 ctttcgacgt tcccgccact tcgggaaccc ccttcccgat caacgtgtgc gcgatgatca
     1321 tctggggttt cccgttgtcg cgctctttcg ccacgttgaa cgcgtcgaga aattgctgca
     1381 tgtcttggcc gtcgatttcc tgcacgtcga agccataggc gcggaagcgc atcccggtat
     1441 cctcgctctg cgtttccttg gccatcgcgt cgagcgtgac agcgttcgag tcgtagatca
     1501 ggatcagatt atcgagtccg aagtgccccg cgaaagcgct cgcttccgcc gagacgccct
     1561 cctgcaaaca accatcgccc gcgaggcaga cgacgtgctg atcgaagatg cggtgctcgt
     1621 cggtattaaa acgcgccgcg gccatcttgc acgccatcgc gatgccagcc gcattggcca
     1681 cgccctggcc taacgggccg gtcgtgcatt cgacgcccgg cgtttccatg aattccggat
     1741 gcccgggcgt cttgctgtgc agttgccgga agcgcttgat ttccgccatc ggcaaatcga
     1801 agccgctgag gtggagccag gcgtaaagaa acatgctgcc gtgcccggcc gagagaacga
     1861 ggtaatcgcg attgatccag cgcggctggt cggggttgta tttcagagcg tagccgtaga
     1921 ggacggcacc catttcggcg cagcccaagg gcaaaccgag atggccggat tgcgaggcgt
     1981 ggacggcatc gatgcaaaga ccacgggcct ggtcggccgc gcgggagagg actgcgagat
     2041 tcaagctcat ttcgccagtc tatatgcgca ggcaggcgtg atgaaagcag agagtttcgg
     2101 cgtttccagc ctaacgaatt cgctcccggt cggagggacg tgcttccgca cgtccgggtt
     2161 ccgccgcggc gttcgcgaaa atcgtggacg tgcggaagca cgtccctccg acaagcctac
     2221 ttcttcggcg tcggcgaagc tatcaccgct ccttctttca agctgaatga ccagtgcgcc
     2281 tcggctttct tcccgccaat cttcgcttcg acgatgacga aacaggattc gccgtgcagc
     2341 ggctgcgtca cctgatacga aaggatcttg ttcgccgcgt cgtagttcgc cggcacgaca
     2401 ccgagtccgc tgacgcgcat ccgcaccgag cccggatcga tccccccgat cgcgccgagg
     2461 ttggccttga tcaatggcct cgaagtcgcc gtcgacccgt cggcgggctg cgggctgatg
     2521 ctggtgatcg gaagctcctc gaccggcgca ctgccgccac ccgacgatcc gccgaagttg
     2581 atcgcgtcct caaacacttt cggtttgttc ccctcgatca tatagcggcc taacgagtcg
     2641 ttcggcgaac cgtaggtcag cttctggcca tagacggtga agaccgcctc gaaattcgcc
     2701 tttttcgtct cttccttgac ccggtcgttg tagtagccaa acggcaccgc gaaacaattc
     2761 actttgatgc cgagcttctg ctcgagcgtc gccttcgagc cgccgacttc gctctcgagc
     2821 cactcgttgt actcctgcgg attcagcttg cgcttggtcg atttgtcgta gggcttgcgc
     2881 aagtcctgat gcgtggcgct gtgtgcttcg atgtcgattc cggcatcacg catctcggcc
     2941 agttgctccc agctcatcgc cgcgccgcca ccatatttcc cgcctttgat tccttcggtg
     3001 tagatgaaga gcgtgaatgg ataattgaac tttttcagga tcggccaggc aacgtcgtaa
     3061 ccggtcttcc agccgtcatc gatcgtgatg acggcacaat gcgacgggat cgctttttcg
     3121 ccgcgtttcc aggcgagaaa atcctgcatg ctaatgaccg cgatcttctt gtccttcagc
     3181 tcctgcatct gcgccgtgaa cgctgcgggc gtaatctcgg tatcgggccg ccgcaccttc
     3241 tcctcgaaac gatgatagcc gaacacgacc acctgcgcgt tctggtcgat cagcggtttg
     3301 cgcggctcgg cggggggcgt cggtgtagcg acgacggcga cggccggcgc cggactcggc
     3361 gtcacgggtt tcacttttcc ggccaggcta tccttcttgc agccgctcaa agccagcccg
     3421 cccagcagca aaaacatcgt caaacggttg cgcataaatc gacgctagcc atcgaatgcg
     3481 accgtggaaa atacaagcga ttattgctcg gtctccggaa gtttcccggc cacgcgcacc
     3541 tttcaatttg cccggcacgc gatccgctct acttgtgctg atgccgcgct cctttgtctc
     3601 cctcgccgcc ctcttgctcg ccgccacttt cctgcttggc ggctgcgcga ctgtcgccac
     3661 caacaccagc cacaccttcg acacggtcgt ggtcgacgcc gggcacggcg gcaaggatag
     3721 cggagcctac cggcgcaatg ccccgccgga aaaaatcgtc gcgctcgatg tcgctctgcg
     3781 ggttgatcgc aaactgcgcg acgcgcattt gaaaaccgtg ctcacacgct cgaacgacac
     3841 tttcatcccg ctcgacagcc gggtcgcgct cggcaacgcg caggagaact cgatcttcgt
     3901 cagcatccat tttaacgact cgcgccggcg cggcgtggaa ggcgttgaaa cctactatca
     3961 ttcaccctac gcctacgatc tcgcggcggc catcgagcgc aatttgtcca ctctgcccgg
     4021 caccgtgaat cgcggcgtgc gcacggcggg attccgcgtc ctgcgcaacg cccactaccc
     4081 ttccgtcctg gtggaatgcg gatatctcag taaccgctcc gaagcgcgcg atgccgcgga
     4141 cgccgattac cgtgaaatgc tggcggacag aatcgcgcag gcgatcgtcg attatcgcta
     4201 cggcggtggc acctatcgcc gtcccgcgat cgcgcagacg agcgcgggcg gcggcgcatc
     4261 ggctgtcccg agcttccgct agagcgtgtc atgaactttt gcgtggccag caaaagccgc
     4321 ggcctgtagc cgtcgacctg tgggcgacgc gggtacgacg gcgtgccagc cgcacacata
     4381 cccacgcttc gcgcagcgaa acggctacag tcgcaaaatg catgaccggc tctagaactc
     4441 gatcccgcgc gcctcgcgcc cgagcgcggc gaggaggatg aggacgacga agaccgccag
     4501 cgtcacgcaa gccatggtga gggcgtaatc cggctggccg tttggcctaa cgaaatgttc
     4561 ggccagcttt gcctggagca acgcggcgtt ggccgagaag aaatttccca gctgatagac
     4621 gacgccgggg aaagtcccgc gccaggccgg cggcgaaagc tcgttcaggt gcaccggcac
     4681 cacgccccac gcgccctgca ccatgaattg catgaaaaaa ccgcccgcga tcaggagcgg
     4741 gagcgtcgac gaaaaaatcc atgccgggat gagcagaatg ccgaagaccg cggcgatgat
     4801 gatcgtccgc cggcgacccc agcgttgcga gtagaagcca agaaccgtgc cgccgcagat
     4861 cgcgcccagg ttgtagatga tcgcgatcgg cgcggtttgg ttaaccgaga gcccgcgctg
     4921 cttttgcagg aaggtcgggt agagatcctg cgttccgtgc gacatgtagt tgaacgcggt
     4981 catcaggagc acggcataaa tcaggagccg ccagttttcg atcaacatcc gccatccgcc
     5041 taacgatttc tccgtcgcgg tggcgtgctg ccgttgccag accgccgatt ccggaacgtg
     5101 ggcacgaatg taaattacca gcaccgccgg gagcgcgccc accacgaaga gcgcgcgcca
     5161 gccaaagtgc ggaaagacga gccaataaac caccgccgcg aggagaaagc cgaaggaata
     5221 accctgctgc aaaatgcccg aggccagccc gcgggcccgc gcgggcagac tctccatcgc
     5281 gagcgacgcg cccaatcccc attcgcctcc catcccaatc ccatagagcg cgcggaagat
     5341 caggaagacg gtgtagttgg gcgagaacgc ggtcagcagc tccatcagcg aatagaaaat
     5401 gatgtcgacc atcagcggga tgcgccggcc gaagcggtcg cctaacaacc cgaaaattag
     5461 cgcgccgagc ggccgcatca tgagcgtgag ggtgatggca aaggttactt tgacgatcga
     5521 gaccgagaac tcggcggaaa cgttccgcac gacaaaggtg aggagaaaga aatcgagcgc
     5581 gtcgagggtc cagccgagaa agcaggcgag aaaggttctg cgttgggttg cgttcagcga
     5641 gcgatagatt tggatcgctt ccacggtcgg catttaactt ttaccgcggc ccgaagggaa
     5701 gctctcatcc tcctcgtgcc cgtgctcgtg cttatgctta tgctcgtatc gaatcggcaa
     5761 ggcatcgcgc tggaaaaaac gattgcgagc aggagcgcga gcatgattac gagaaagtga
     5821 aacagacgca ttgacgcatc atgatacgtc aatttgttag ttgctgagcg ggcccgactg
     5881 tcgttaattg ctccttcgct gtgcacaccg cctcgaccca tcccctcgcc gcgctcctcc
     5941 gccagcgcat cgtcatcctt gacggcgcca tgggcacgat ggtgcagcgc cacaaattat
     6001 ccgaagagca gtaccgcggc gctcgctttg ccgctcatcc tgcgaagcac ctgaaaggcg
     6061 atctcgaact gctccagctc acccagccgg aagtgatcga gacgattcac ctccaatacc
     6121 tcgaggccgg tgccgacatc attgaaacca acaccttcag cgccaccacc atcggccagc
     6181 acgatttcct ttttgcgggc gaaccggaga gcgggcgcaa ggacgccgcc ttttttgagc
     6241 gcgtcgtcgc ggattcggag ctcgccgcgc tcgtttccga aatgaatctc gccgcggcca
     6301 gggtggcgcg cgccgtcgcc gatcgcgtcg cgcaccagac cggccggccc cgcttcgtcg
     6361 ccggttcgtt aggcccgctc cccgtcaccg cctccctttc gccggatgtg aacgatccga
     6421 gttttcgcgc ggttacgttc gatcaaattc gccgggccta cggcgagcag gcgcgcgcgc
     6481 tgcttgaggg cggggtcgac ctccttctcc tcgaaacgat tttcgacacc ctcaacgcca
     6541 aggccgcgct ctttgccatc gccgaggtct tcgagtcgtt cggcaagaaa ttgcccgttc
     6601 tgatttccgg cacgattact gatctctccg gccggactct cacgggccaa accgtcggcg
     6661 cgtttctcac ctccatcctg cacgccgagc cgctggtggt gggattgaac tgcgcgctgg
     6721 gcccggcgga gatgaggccg cacgtcgagg aaatggcccg cctcgcgccc tgttttacga
     6781 gcgcttatcc caacgccggc ctgcccgatc cgctgtcgcc gaccggattt cctgaaaccc
     6841 cggaatcgct cgcgccgcaa ctgcgtgagt gggcgaaaaa cggctggctc aacatcgtcg
     6901 gcggctgctg cggcacgacc ccggaacaca tccgcgcgct ggcagaggcg gtgcgcgatt
     6961 gcccaccgcg cgttctgcct ctgtggagcg cgcgcgcccc gtcccgcggt ggcgggattt
     7021 ccggcgcctc gcgggaaaca tcttcgcacg aagtttcagg gttgaacgtt gattcatctt
     7081 catacgcagt agaagaagtt cgcggcgagg cgccgcgaac agcacgcgag gcgcgtgcgc
     7141 tccccggacc agaggcaccg ctccaccttg ccggactcga agccctcgat gtcacacccg
     7201 acttcggctt tgtcgtcgtt ggcgagcgga ccaacatcac cggctcgccc aaattttcga
     7261 agctgatcct ggcgggcgac ttcgaaggcg cgctcgtcgt cgcgcgccac caggtcgaag
     7321 gcggcgcgaa catcctcgac gtcaacttgg acgaaggcat gattgattcc gaggcggcca
     7381 tgacgcgctt cctccacctg attgggagcg agcccgacat ctcgcgcctg ccgatcatga
     7441 tcgacagctc gaagtggagc gtgctcgagg ccggcctgaa gtgcgtccaa ggcaaagggg
     7501 tcgtcaattc catctcgctc aaggcgggcg aagaggaatt tctgcgccag gcgcggctcg
     7561 tcaggcgtta cggtgcggcc gttatcgtga tggccttcga tgaacgcggc caggccgaca
     7621 cgtttgcgcg caagaccgag atctgcaccc gcgcctaccg gctcctgacc gaagcggccg
     7681 gctttccgcc taacgacatc attttcgatc ccaacatcct gacgatcgcg accgggctcg
     7741 aagagcataa cgaatacgcg attgcctttg tcgaagcgac gcgctggatt aaacagaatc
     7801 tcccgggagc gcgggtcagc ggcggggtga gcaacatttc cttttccttc cgcggcaaca
     7861 acacggtgcg cgaagcgatg cactccgcct ttttgtatca cgccatccgg gccgggctcg
     7921 acatggcgat cgtcaacgcg ggtcagctcg ccgtttacga ggagatcgac ccgcctttgc
     7981 gcgagctggt cgaggacgtg cttttcaatc gccgtccgga tgcgaccgag cgcttggtcg
     8041 atttcgccga gaaagtaaaa tcgcaaggga aagtcgcgcc ggatgaagaa atctggcgga
     8101 gcgcgccggt cgaggagcgg ctcgcgcacg cgctcgtcaa agggatcgtc gagcatatcg
     8161 aggccgacac cgaagaggcg cggcaaaaat cggcgcgccc gctcgatgtc atcgaagggc
     8221 cgctcatggc ggggatgagc gtggtcggcg atctgttcgg ctccggcaaa atgtttctgc
     8281 cgcaggtggt gaagagcgcg cgggtgatga agaaggccgt cgcctacctg atgccctata
     8341 tggaggcgga gaaacaggcc ggtgcaaaac cgcaggggcg gatcgtgatg gcgacggtga
     8401 agggcgacgt gcacgatatt ggcaaaaaca tcgtcggcgt cgtgctgcaa tgtaacaatt
     8461 acgaggtcat cgacctcggc gtgatggtgc cggcggaaaa aatcttcgag accgcgcgcg
     8521 cccggcaggc aaacatcatc ggattgagcg ggctcatcac gccttcgctc gatgagatgg
     8581 tgcacgtcgc gcaggaaatg gagcgacagc aatttcgcct gccgttgctg atcggcggcg
     8641 cgacgacgag ccgcgcgcac actgcggtca aaattgcgca gcattatcac gagagcgtgg
     8701 tccacgtgct cgacgcctcg cgcgcggtcg gagtagtgag cgcgctcctc aacccggacg
     8761 cgaagctgaa gttcgatgcg accacgcggg ccgattacga acgactccgg accgagcacg
     8821 ccaaccggac gcgcgagaaa aaaatgctca ccctggcgca ggcgcgaacc aatcgcctaa
     8881 cgatcgactg gagcagttat gagccgcccg cgcccgggtt tcttggagtg cgggtcgtcg
     8941 gaactccggg gagcgcacgc gccccgcgtg ctgtttccgg cgcctcgccg gaaacatcga
     9001 gggagggcga catccattac tcgaaacgcc ggctccctca tttcgagcgt ccgtggagca
     9061 agtacgcggt cgtcttctcc actcacgaac gtcgcccgct gcacccttcg gaacgcgacc
     9121 tcgtcctgca atcaatcctg acggctcacg aacgtaacca aatcgagctc tacgccgcgt
     9181 gcgtgatgcc ggaccacgtt cacctgttgt tcgagccgca gatcaaacaa agcgcggccg
     9241 acggcagcgc cgtcttttgg cctttaaccg acatcctccg agggatcaaa tcgagcaccg
     9301 cccatcggat caacaaggct gccggtcgca ccggcccagt ttgggaacga gaatccttcg
     9361 atcggttgat tcgatcggaa tcggatctgc aggagaagtt tgactacatc atgaagaatc
     9421 cctggacttc agaaatcgtt aggagagacg aggactatcc ctggctttgc acaccaaaga
     9481 cttctcggcg aggcgccgag aagagcacgc gaggcgcgtg ccctccccag acgacgcgca
     9541 actcgcaagc tacttcggtc gtcgaaatct cgctcgagac tttggtcgag tacatcgatt
     9601 ggtcaccgtt tttccatact tgggaattgc gcgggcgcta tcccgcgatc ttcgatgatc
     9661 ccgtggtcgg cccacaggcg cgcgagcttt tcgccgacgc gcaaaaactg ctcgaccgaa
     9721 tcgtcgcgga aaagctgctc accgcccgcg gggtctacgg tttctggccc gccaactcgt
     9781 taggcgacga cgtcgagatt tatcgtgacg agttacgctc cgaggtgctc gcgcgctttt
     9841 attttctccg gcagcaaatg cagaaaccgg cgggacaaag caactactgc ctggccgatt
     9901 ttgtcgcgcc caagggctcc ggccggcgcg attatcttgg cgggttcgct gttaccgccg
     9961 gcctcggggc ggacgacctg gcgaaacatt ttcagggcga gctggacgac tacaacgcca
    10021 tcctgacgaa ggccctggct gatcgcctgg ccgaagcgtt cgctgaatat ctacaccgcg
    10081 aggcgcgcct cgcctggggt ttcggcgcgg aggaaggcct cacaaccgat gatctgattc
    10141 gcgagcgcta ccgcggcatc cggcctgcgc ccggttatcc ggcgtcgccc gatcacgtcg
    10201 agaagcgcac gctcttcgat ttgctcgggg cggaagacag cgcgggcatc accctgacgg
    10261 aatcattcgc catgcatccc ggcgcaagcg tcagcgggct ctatttttcg catcccgcag
    10321 cgcgctattt tgcggtcggg aaaatcgagc gcgaccaggc gaccgattac gcagcacgga
    10381 aaggcgagtc gttaggcgag atcgaaaaat ggctctcgcc gaatttgaac tacgagccga
    10441 agcgagaggc ggcgcgttga gcgcagacgt cgggtctaag atcgcggtga ggagaatcga
    10501 gcggctcgat tccgggaggc gctgaaattg ccgggcaaaa aaggccacga tcgcccgcca
    10561 ctaaacctgc tgattccgat ttggcccagt ggtttttcca gccggtgacc agacgcgaac
    10621 gggacgcagc ctgtcgtcca ttcgtcaggc aagcggagcg cttgccctac agcaccgagt
    10681 tcggatcggc gctcgtgatc agcatggtgc cggcttcatc ggcggagatc tcttcgactt
    10741 ccttttccgc cgctttgggc tcgggccaga cgacgttcca ggcgagatgg agcttttcga
    10801 gcatccagat ggtgtagcgg gtaaaatcga actcccacca cttcaacccg tgcgccgcgc
    10861 aacgcggctt ggcgtgatgg ttattgtgat aaccttcgcc cagcgcaccc aggccgaccc
    10921 agaagttgtt cttgctctcg tccgacgttt cgaaactgcg ataaccaaac tgcttcatgt
    10981 ggcagatcga attcaccgcc caggtgcagt tgtagatgat caggatcggc acataaagac
    11041 accagagcat tccggccaga ccgacggtgg cgaaaaggag gctggcgacg gcgagatgcg
    11101 ggagcacata aagcgcggaa ttttcccaca gtttgcagta ccagattctg cgcacgtcgg
    11161 ccgcataacg cagcgaacgc tcgcgaaagg aggccggttt gcgaatgagc cacatcatgt
    11221 gggcataagg aaatccgtgc cggggactat ggatgtcttc ttcggtgtcg gatttaagat
    11281 gatgaaaacg atgatcgccg acccaggtga ccgggtcacc cgagagaccg atagccgagc
    11341 cggtggcgaa aataaatttc aaccaacctg gcatttcgaa tccgcgatga gtcaggtaac
    11401 gatgcaaata aagcgtggtc ccgagcccga tccaaagata gaggacggcc atgatcggca
    11461 gatagaaagg atgaaagcgg gtccagacag ccgcgacacc gagcagctgc agcaggacga
    11521 gaatcgtgaa aatggcaata ttgatttttt cgtttttgaa cataaacagg gcttaagcag
    11581 attgccggcc gtcggagaga ggaagcccgg agtgtcgctg ggaagtgcct gacgcgcaac
    11641 cgttatttgc ccgaccccgg cgggccgcga caggtcgctc gcaagaaaag ttcacgtgcg
    11701 ccccgcctct ttgcgtgctt ttagggcagc attttcccgg ggtgtagctt agcttggtag
    11761 agcggtcgcc gcggcgacag gtcgccccaa aagttcacgt gcgccccgcc tctttgcgtg
    11821 ctattagggc agcatattcc ccggggtgta gcttagcttg gtagagcgcc tggtttggga
    11881 ccaggaggtc gcaggttcaa atcctgtcac cccgattttt catctgacat gttttcggaa
    11941 tgccctggac tctcattcac gcagaacaat tcccgacacg cgcggcggcc gccgcgcgag
    12001 aacgctactt taaaactggc cagggacgtg atgaacttga tcaccttcag cgggccgagc
    12061 ggtcgccgcg gcgacaggtc gcagcttcaa atcctgtcac cccgattttt catctgacat
    12121 gttttcggtt tacgtcttaa aaagcggagc gacggggaga cggtacgttg gttcgtgtgc
    12181 caacctttcg gaccgtctgc gaaggcacaa cgcaggcgag tctctcgcaa ccaaacacgg
    12241 aatgcccggg actctcattc acgcagaaca attcccgaca cgcgtggcgg ccgccgcgcg
    12301 agaacgctac tttaaaactg gccagggacg tgatgaactt gatcaccttc agcgggccga
    12361 gcggtcgccg cggcgacagg tcgcagcttc atgtattgtc accccgacac cagagtttcc
    12421 cggcgacgaa agatgaattt tttccgccag ctgatccttg gaatgacgtt cgttttcgcc
    12481 gcgcttctat gcgcgcaatc gccacctgct tcggacaact ccgcaccggc gaccccagcg
    12541 ccatcgccca cggccccgat cgaaccaccc tctctcattc cgccgaacat tctgcccggc
    12601 ccgggaatcg gagcgcttcc ccagatgccc gccgccgccg atttgcagat gctcaacgcg
    12661 cttttcaaac agacatcgct cggcaaggcc gccgatgact atcggctgca cgtccaggtg
    12721 gtgcgactcg aaagccagat tcgaaacgac gcggagctcc aggcgatgag agcttcggcc
    12781 gagcaaacgt cgaccgatct cgaaaagcga aaacggctga aggcgtatta tcaccaatac
    12841 tacagccggc ttcggacgat ggcggacacg ccggacttaa aggtgtatct cgacgggcag
    12901 gaagccgcac acgccgggtt gctcgcgcag ccgcgcgtgc gcccgaacac aaatcccgtc
    12961 gcggccagcg ccaccgcgac gccgctgccg acgccggtcc aggcgcgcgc cggcgccgtg
    13021 cagcggcacc cctgagcagc aggcacgcga cgaacctggc cggaaccaaa tcggacggtt
    13081 gccgccgcga gggagaccgg ctaggatggc ggagcgaaaa cggcgcattc gtctagtggt
    13141 taggacacag ccctctcaag gctggtgcac gggttcgatc cccgtatgcg ctgccattgc
    13201 actcgattta gataaaatgg gtgcaggcgg aatcatcatt cgctggttcc tattgatctt
    13261 cagctcctta cgaatatcgt cggcct
//
LOCUS       DATMNS010000326         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Chthoniobacterales bacterium isolate SMAG_U12856
            k141_4649829, whole genome shotgun sequence.
ACCESSION   DATMNS010000326 DATMNS010000000
VERSION     DATMNS010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943463
            Sequence Read Archive: SRR7097863
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Chthoniobacterales bacterium (soil metagenome)
  ORGANISM  Chthoniobacterales bacterium
            Bacteria; Verrucomicrobiota; Spartobacteria; Chthoniobacterales.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 18:13:15
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,278
            CDSs (total)                      :: 2,253
            Genes (coding)                    :: 2,248
            CDSs (with protein)               :: 2,248
            Genes (RNA)                       :: 25
            rRNAs                             :: 1, 1 (16S, 23S)
            partial rRNAs                     :: 1, 1 (16S, 23S)
            tRNAs                             :: 21
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 5
            CDSs (without protein)            :: 5
            Pseudo Genes (ambiguous residues) :: 0 of 5
            Pseudo Genes (frameshifted)       :: 0 of 5
            Pseudo Genes (incomplete)         :: 5 of 5
            Pseudo Genes (internal stop)      :: 0 of 5
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Chthoniobacterales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4649829"
                     /isolate="SMAG_U12856"
                     /isolation_source="soil"
                     /db_xref="taxon:2201154"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.481016 N 72.178343 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..1544)
                     /gene="atpA"
                     /locus_tag="VNW28_11365"
     CDS             complement(12..1544)
                     /gene="atpA"
                     /locus_tag="VNW28_11365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXA10571.1"
                     /translation="MSSILEEIETQIAGLKTSTTKSNVGIVREAGDGVARVEGLSEVM
                     LNEMIEFPSGDFGLALNLEETEVGVILLGDPTKVSEGDEVKTTGRLLQVPVGKALLGR
                     VVNTLGQPVDNKGPIHTDTFYPVEKIVPGIIKRRSVSQPVQTGIMAIDAMVPIGRGQR
                     ELIIGDRATGKSTIAVDTIISQARLNKAAEDGQLKDHRPLYCIYVAIGQKNSNVARTL
                     AVLEKNGAMPYTTIISAPASESATNQYLAPFAGAAMGEWFMDNGMDALIIYDDLSKHA
                     VAYRQVSLVLKRPSGREAYPGDVFYLHSRLLERSARVTEESGGGSLTALPIIETQAGD
                     VSAYIPTNVISITDGQIYLETDLFFQGVRPAISVGLSVSRVGSAAQIKAIKQVAGKIK
                     LDLAQFRELAAFAQFGSDLDAATKARLDRGQRIVELFKQIQYNPIPVEEQVAVLWAMQ
                     NGYLDPVPVERVKEFQAKLQDYLQTRKESVLAAIRTKRAIDKDLEADLATALSDFKAT
                     WQ"
     gene            complement(1559..1951)
                     /locus_tag="VNW28_11370"
     CDS             complement(1559..1951)
                     /locus_tag="VNW28_11370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HXA10572.1"
                     /translation="MKISKDVRQLSRKLVRASFVDGVLDSEKIKSLVQSIIAQKPRSY
                     LQLLENYQRLLRLEVEKQTATIESATELDPDAGRQIADGLRRKYGKGLTTEFVVNPAL
                     LGGVRIRVGSDVWDSSVRNRLDRLQQKL"
     gene            complement(1970..2503)
                     /gene="atpF"
                     /locus_tag="VNW28_11375"
     CDS             complement(1970..2503)
                     /gene="atpF"
                     /locus_tag="VNW28_11375"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HXA10573.1"
                     /translation="MNIPIFLAANLVTDTAETFGFDVKIFLSQVISFVLVALLLKQFA
                     YKPILAVLEERRQRIAEGLLNAEKIKQQLAEAEQRYQEILAKGNAEAQRMIDEARASA
                     GVIAERKQQEAIVAAEQILAKAREASALEHERTMSQLKRELGRLVIDTTAKVTGKVLT
                     REDQRRLQEEASRQVAS"
     gene            complement(2545..2760)
                     /locus_tag="VNW28_11380"
     CDS             complement(2545..2760)
                     /locus_tag="VNW28_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HXA10574.1"
                     /translation="MILPLLAEMSGSIHVGLAALGAAIGVGLIGMGAASAVGRNPGAA
                     TPILVQAILAIAFAEAIVFYALFLVPK"
     gene            complement(2827..3765)
                     /gene="atpB"
                     /locus_tag="VNW28_11385"
     CDS             complement(2827..3765)
                     /gene="atpB"
                     /locus_tag="VNW28_11385"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HXA10575.1"
                     /translation="MINRSFRQLPLLLSVLAMLIFSGLASVAQAQETIAPETGAVAEK
                     HEAIPLKAAPLFTIGEFTVTNSMLVTWVVALGIIVFAQTATRKIKQVPTGAQNFWEWL
                     VEGLYNFLESVIGSHLVKKTFWFFATIFIFILFLNWFGLLPGVGTVGWGQHDPVTNVF
                     HVDRPLLRGANADLNLTFAMAATFFALWIFWALQENGIGGFIMHLFGPKGDSKGILKV
                     LMVVVFFMVGWLEIVSILFRPISLSFRLYGNVYAGENMLESMAHIVPALSWLIPIPFY
                     FMELLVGIVQALVFMLLTAVFTLLIAQHEPAQGAHH"
     gene            complement(3955..>4566)
                     /locus_tag="VNW28_11390"
     CDS             complement(3955..>4566)
                     /locus_tag="VNW28_11390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXA10576.1"
                     /translation="PKKGLTTCLRAFAVFRRENPGAELLIAGKGPLQPVLEALAAQLD
                     ISDQVHFTGFLSQPDLLALYHSCHVFLHPSEMPADENQEGIPNSILEAMATGMPVLAT
                     RHGGIPEAVEEGRCGALVEERDYLALAAEMKKITRSPHSFGEIGVLASESVAAHFEQR
                     LHIAQLESHYREALEIAHEAVARERSEHPVTAPQFQPERVAAK"
BASE COUNT          892 a         1366 c         1362 g          946 t
ORIGIN      
        1 ctgtcaggct gctattgcca ggtcgccttg aaatcgctca gcgccgtcgc caggtcggct
       61 tccaagtcct tgtcgatcgc ccttttcgtc cggatggcgg cgagcacgct ttctttgcgc
      121 gtctggagat aatcctgcaa cttcgcctga aattccttta cccgttcgac aggcaccgga
      181 tccaaatagc cattctgcat cgcccaaagc accgcgacct gctcttcgac cggaatcgga
      241 ttgtattgaa tttgcttgaa cagttcgacg atccgctggc cgcgatcgag ccgcgctttg
      301 gtcgcggcgt ccagatccga accgaactgc gcgaaggccg ccagttcgcg gaactgggcg
      361 aggtcgagct taatcttgcc cgccacctgt ttgatcgcct tgatctgggc agccgaaccg
      421 acccggctga ccgaaagacc gaccgagatg gccgggcgga cgccctggaa aaagagatcc
      481 gtctcgagat aaatctggcc atcggtgatc gagatgacgt tggtcggaat gtaggcggac
      541 acgtcgcccg cctgcgtttc gatgatgggc aacgccgtta acgagccgcc gccggattcc
      601 tccgtgacgc gggcactgcg ctcgagaagc cgcgaatgca gataaaaaac gtcgcccgga
      661 taggcttcgc gaccggaggg acgtttcaaa acgagcgaca cttgacgata agcgaccgcg
      721 tgtttcgaaa gatcgtcgta aatgataagc gcatccatac cgttatccat gaaccattca
      781 cccatggcgg caccggcaaa cggcgcgaga tactggttcg tcgcgctttc ggaggcgggc
      841 gcggaaatga tcgtcgtgta gggcatggcg ccgtttttct cgagcaccgc gagggtccgc
      901 gcgacgttcg agtttttctg gccaatcgcg acgtagatgc aatagagcgg ccgatgatct
      961 ttcaactggc cgtcttccgc cgctttgttc aagcgcgcct ggctgatgat ggtgtcaacg
     1021 gcgatggtcg atttgcccgt ggcgcgatcg ccgatgatca gctcgcgctg gccgcgaccg
     1081 atcggcacca tcgcgtcgat cgccatgatc ccggtctgga ccggctggct gacgctccgg
     1141 cgtttgatga tgccgggcac aatcttttcg acggggtaaa acgtgtcggt gtggatcggc
     1201 cctttattat caaccggctg gcccaaggta ttgaccacgc ggccaagcag cgccttgccg
     1261 accggaacct ggagcagtct tcccgtcgtt ttgacttcat cgccttccga gactttcgtc
     1321 gggtcgccga ggagaatgac gccgacctcg gtctcctcga ggttgagcgc gagaccgaag
     1381 tcgccacttg ggaactcgat catttcattc aacatcacct cgctcaggcc ttcgacgcgc
     1441 gcgacgccat cgcccgcctc gcggacgatg ccgacgttgc tcttggtggt cgaggttttc
     1501 agacccgcga tttgtgtttc gatttcttcc agaatgctgc tcatggtaat tcgttaggtt
     1561 aaagtttctg ctgcagacga tcgagtcggt tgcgcacgct gctgtcccag acgtcgctcc
     1621 cgacgcggat gcgcacaccc cccagcaggg cggggttgac gacaaactcg gtcgtcaggc
     1681 ccttgccgta tttgcgtctc aatccgtccg cgatctgccg gcccgcatcc gggtcgagct
     1741 cggtggcgga ttcgatcgtg gccgtctgtt tctcgacttc caggcgcaac aagcgctgat
     1801 agttttcgag aagttgaaga tagctgcgcg gtttttgcgc gatgatcgac tgaacgagcg
     1861 atttgatttt ttccgaatca aggacgccat cgacgaagct cgcgcgcacg agtttgcgcg
     1921 agagttgacg gacgtctttg ctgattttca cgctcttcgg tcgatctcgt taggatgcca
     1981 cctgccggct cgcttcttcc tggagccggc gttgatcctc gcgcgtcagg actttgccgg
     2041 tcactttggc ggtggtatcg atgaccaacc gcccgagttc gcgtttcagt tggctcatcg
     2101 tgcgctcatg ttcgagcgcg ctcgcttccc gcgccttggc cagaatttgc tccgccgcga
     2161 cgatcgcctc ctgctgcttg cgttccgcga tcacccccgc gctggcccgc gcttcgtcga
     2221 tcatcctctg cgcttcggcg ttgcctttcg ccaggatttc ctggtaacgc tgttccgctt
     2281 cggccagctg ctgtttgatt ttctcggcat tgagcaggcc ttcggcgatg cgttgccggc
     2341 gctcttcgag gacggcgagg atcggcttgt aggcgaactg cttcaggagc agggccacga
     2401 ggacgaagct aatgacctgc gagaggaaga tcttgacgtc gaagccaaaa gtctccgccg
     2461 tatcagtgac cagattggcg gcgagaaaga taggaatgtt catccgaagt taggcggcgc
     2521 ggagcgggat gctcttgcgc ttgatcattt tggaacgagg aacagagcgt agaacacgat
     2581 cgcttccgcg aaagcgatcg cgaggatcgc ctgcaccagg atcggggtgg ccgcgcccgg
     2641 attgcggccg accgcggacg ccgcgcccat gccgatcaat cccacgccga tcgccgcgcc
     2701 aagggccgcg aggccgacgt gaatgctgcc gctcatctct gccagtagtg gtagtatcat
     2761 agttttcttt tggttggttt gcttcagcca cctctccgcg gggtgcgcgg cggtggccga
     2821 aattagttaa tggtgtgcgc cttgcgccgg ttcgtgctgc gcgatgagca gggtaaagac
     2881 ggcggtcaac aacatgaaga ccagcgcctg cactatgccc acaaggagct ccataaagta
     2941 aaacggaatc gggatcagcc acgagagcgc cggcacgatg tgcgccatcg attcgagcat
     3001 gttctcaccg gcgtagacgt tcccgtaaag ccggaaactg agcgagatcg gccgaaacaa
     3061 aatcgaaacg atctccagcc agccgaccat aaaaaagacg acaaccatga gcaccttgag
     3121 aatgcccttg gaatcaccct tcggtccaaa aaggtgcata atgaatccgc cgatgccgtt
     3181 ctcctgtagc gcccagaaga tccagagcgc gaaaaaagtg gccgccatcg cgaaggtgag
     3241 attgagatcc gcattcgccc cgcggagcaa aggacgatcg acatggaaga cgttcgtcac
     3301 cggatcgtgc tgcccccagc cgactgtccc caccccgggg aggaggccaa accagttgag
     3361 gaaaaggatg aaaatgaaaa tggtggcgaa gaaccaaaat gtttttttca ccaagtgcga
     3421 gccgatcacg ctctcgagaa agttgtaaag cccctcgacc aaccactccc agaagttctg
     3481 cgcgccggtc gggacctgtt tgattttacg cgtcgcagtc tgggcgaaga cgatgatgcc
     3541 gagcgccacc acccaggtga cgagcatcga gttggtcacg gtgaattctc cgatcgtgaa
     3601 aagtggcgcc gctttcaggg gaatcgcctc gtgtttctcc gcgaccgcgc ccgtctctgg
     3661 cgcgatcgtc tcctgcgcct gcgcgacgct cgcgaggccg gagaaaatca gcatggcaag
     3721 gacgctcagg aggagcggca attgacgaaa agagcggttg atcatgaatg gctttgtcga
     3781 gaagcggcgc ctccgacatc cggcaggcgt cgcacttttc ctggagagaa agggcgcgcg
     3841 ccccaaggcc gcttacggcg cgcatacaga agagcatgtt gcccgcgctg acaagcaact
     3901 ttcgggagtt tttttcggcg cgaaagcgag gccgaaagat caggccccag cccgttattt
     3961 cgccgctacg cgctctggct gaaactgcgg cgccgtcacc gggtgctcgg atctctcacg
     4021 agcgacagct tcgtgcgcaa tttccagcgc ctcgcggtaa tggctctcca gttgcgcgat
     4081 gtgcaagcgc tgctcgaaat gggcggcgac cgattcgctc gccaacacgc cgatctcacc
     4141 gaaggaatgc ggcgagcgcg tgatcttctt catctcggcc gccagcgcca gatagtcgcg
     4201 ctcctcgacc aacgctccgc agcggccctc ttccaccgct tcgggaatcc cgccgtgccg
     4261 cgtcgcgagg accggcatgc cggtcgccat cgcctcgagg atggagttgg gaattccctc
     4321 ctggttttcg tccgctggca tttcgctcgg gtgcaaaaat acgtggcaac tgtggtagag
     4381 cgcaagcaga tccggctgcg aaagaaaacc ggtaaagtgc acctgatcgc tgatgtccaa
     4441 ttgcgcggcg agcgcttcga ggacgggttg caaaggcccc tttccggcga tcagcagctc
     4501 tgcccccgga ttctctcgtc ggaaaacggc aaaggcgcgc aaacaagtcg ttaggccttt
     4561 tttcgg
//
